Hft.
                                                                       EPA-600/4-81-005
                                                                       February  1981
                                    DEVELOPMENT OF A HELICOPTER
                             WATER QUALITY MONITORING/SAMPLING SYSTEM

                                                by

                                         H. Michael Lowry
                               Advanced Monitoring Systems Division
                            Environmental Monitoring Systems Laboratory
                                     Las Vegas, Nevada  89114
        EJBD
        ARCHIVE
        EPA
        600-
        4-
        81-                 ENVIRONMENTAL MONITORING SYSTEMS LABORATORY
        005                    OFFICE OF RESEARCH AND DEVELOPMENT
                              U.S. ENVIRONMENTAL PROTECTION AGENCY
                                    LAS VEGAS, NEVADA  89114

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                                   DISCLAIMER


     This report has been reviewed by the Environmental  Monitoring Systems
Laboratory, U.S. Environmental Protection Agency, and approved for publica-
tion.  Mention of trade names or commercial products does not constitute
endorsement or recommendation for use.

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                                                             Repository Material
                                                            Permanent Collection
                                       SUMMARY


         USEPA-EMSL-LV has developed a helicopter borne  water quality monitoring/
    pump-sampling  system for sampling lakes and rivers.   Development of this
    sampling  system originated at the beginning of the National Eutrophication
    Survey (NES) in May of 1972 for use in Bell UH-1H, "Huey" helicopters
    (outfitted  with floats), but the design concepts  apply  to any aircraft.
    Development continued intermittently, while the system  was  in use, for the
    next 5 years.  This document reports the problems encountered in our original
    sampling  effort and the design concepts applied to correct  those problems.

         The  basic system, originally designed for ship-board use (InterOcean
    model  513 with pump sampler), was mechanically tailored to  fit our aircraft.
    Components  were replaced or modified as deficiencies became apparent.

         Water  samples of any volume can be collected to a  maximum depth of 172
    meters at a rate of 7 liters/minute and in-situ measurements can be made of
    conductivity, temperature, sampling/measuring depth, dissolved oxygen, pH, and
    turbidity.

         From our experiences with this sampling system  we  have concluded that:

         -- helicopters equipped with floats are versatile, cost-effective
           sampling platforms;

         -- this sampling/measuring system is reliable when properly maintained;

         -- the pump-sampling concept is the desirable way  to collect multiple
           samples from multiple depths in the water column to a depth of about
           350 meters;

         -- electrical interferences and vibration problems are hazards when
5?         using water quality monitoring systems in an aircraft;
to
         -- dissolved oxygen and pH sensors are extremely vulnerable to mechanical
           damage and electrical interference, and are  of  questionable utility
           for use with an airborne system; and

         ~ to  ensure continued reliable operation, water quality monitoring
           systems require frequent calibration and  maintenance.
                                                 u   *        USEPA
                                                 Headquarters and Chemical Libraries
                                                      EPA West Bldg Room 3340
                                        711                 Mailcode 3404T
                                                      1301 Constitution Ave NW
                                                       Washington DC 20004
                                                           202-566-0556

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iv

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                                   CONTENTS
Summary	     Ill
Figures	     vii
Tables	    viii
Abbreviations and Symbols 	      ix

Introduction	       1

Conclusions		       2

Recommendations 	       3
  General  recommendations 	       3
  Specific recommendations	       3

Water Quality Sampling Platforms	       5
  Sampling platform requirements and options	       5
  Helicopter sampling platform	       6

Helicopter-Borne Water Sampling System	       9
  In-situ sensors and pump	       9
    Assembly	       9
    Junction box	      12
    Pump	      12
    Sensor and analog electronics 	      15
  Hose/cable, boom, sheave and winch  	      20
    Hose/Cable	      20
    Boom	      21
    Sheave	      21
    Winch	      21
  Onboard electronics 	      25
    Mounting rack	      25
    Console	      25
    Graphical  displays	      25
    Winch controller	      27
    Pump controller	      27
    Power supplies	      27
    Echo sounder	      27
  Sampling manifold and sample storage	      28
  Other underwater components 	      28
    Solar illuminance meter and secchi disk	      28
    Sediment sampler	      29
    Echo sounder transducer 	      29

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                              CONTENTS (Continued)

                                                                      Page
Sampling Operations 	     30
Calibration and Maintenance 	     32
Sampling Capabilities 	     36
Discussion	     38
Appendices
    A.  The Dissolved Oxygen Circuit	     41
    B.  The Differential-Input pH-Measuring Circuit  	     44
    C.  Water Column for Calibration of Echo Sounder	     47
    D.  Calibration Buffer Board	     49
Literature Cited	     51
                                     VI

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                                FIGURES

Number                                                                   Page

   1  In-situ sensor/pump assembly being deployed from the "Huey"
        helicopter while on station 	    7
   2  Floor plan of the helicopter with the monitoring/sampling
        system located	    8
   3  Block diagram of the helicopter water quality monitoring/
        sampling system 	   10
   4  Plan view of the in-situ sensor/pump assembly showing
        relocation of the turbidity sensor	   11
   5  In-situ sensor/pump assembly with components identified .....   13
   6  Hypro model B-612 positive displacement pump	   14
   7  Inductive conductivity sensor (InterOcean)	   16
   8  Dissolved oxygen sensor (Yellow Springs Instruments)	   18
   9  Process Lazaran reference and glass pH-measuring electrodes
        (Beckman)	   19
  10  Hinged boom mounted in the helicopter	   22
  11  Light-weight sheave for the sensor hose/cable 	   23
  12  New 100-meter winch mounted in the helicopter 	   24
  13  Onboard electronics subsystems mounted in the helicopter	   26
  14  Dye study of helicopter rotorwash-turbulence effects on
        Lake Mead	   40
                                     vii

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                                    TABLES


Number                                                                  page


   1   The Parameters and Types of Sensors Used on the  InterOcean
         In-Situ Sensor System 	     9
   2   Daily Calibration Procedure for Helicopter Borne Water
         Quality Monitoring Package 	   33

   3   Daily Operational Checks of the In-Situ Sensor/Sampling
         Package	   35

   4   Specifications of the In-Situ Water Quality Monitoring
         Package	   37
                                    vm

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                           ABBREVIATIONS AND SYMBOLS
A        —   ampere
a.c.     --   alternating current
cm       —   centimeter
d.c.     --   direct current
DO       --   dissolved oxygen
ft       --   feet
FTU      --   formazine turbidity unit
g        --   gram
gpm      --   gallons per minute
gal      --   gallon
hp       —   horsepov/er
in       --   inch
10       --   InterOcean
kg       --   kilogram
kw       —   kilowatt
1         —   liter
1pm      --   liters per minute
Ib       --   pound
m        —  meter
mm       —  millimeter
psi      ~  pounds/square inch
rpm      --  revolutions  per minute
V         --  volt
VA       --  volt ampere

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                                  INTRODUCTION


     In the spring of 1972, the U.S.  Environmental  Protection  Agency's
Environmental Monitoring Systems Laboratory at Las  Vegas (EMSL-LV)  initiated
the National Eutrophication Survey (NES),  a 4-year  field investigation  of the
effects of nutrients on the aging process  of the nation's lakes  and
reservoirs.  The program was designed to sample each  of 815  lakes,  distributed
nationwide, once each during spring,  summer, and fall  seasons  (USEPA  1974,
1975).  It was necessary to collect water  samples for laboratory analyses that
would best characterize the individual  water bodies in terms of  nutrient
levels and their manifestations, e.g.,  chlorophyll  ^concentrations and
phytoplankton composition.

     To sample this large geographical  area in 4 years and to  visit each  lake
three times in the same year, aircraft  were considered the only  practical
survey platform.  After some field experience with  both helicopters and
fixed-wing aircraft, helicopters were chosen as the most versatile  and
cost-effective survey platform to accomplish our mission. No  commercial  water
quality monitoring/sampling system designed specifically for use from an
aircraft was available, nor was there time for system development before
beginning the field effort.  To execute tha rigorous  schedule  of the  field
study, a ship-board system was procured and subsequently modified for airborne
application during the survey.

     This report describes the helicopter  water quality sampling system
developed originally for use by the NES and subsequently used  in support  of
other water quality projects.  It also  describes the  salient problems
associated with the use of a helicopter as a sampling platform and  the
modifications made in the system to fulfill  our needs.  This system should be
useful  to other groups involved in sampling large numbers of water  quality
sites distributed over large or inaccessible areas.

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                                  CONCLUSIONS


1.  Our experiences indicate that helicopters equipped with floats are
    versatile sampling platforms and are cost-effective when large or
    inaccessible geographic areas must be sampled quickly with few personnel.

2.  The sampling/measuring system described here is reliable when properly
    maintained.

3.  The pump-sampling system is desirable when multiple water samples of
    variable volume are to be collected from many different depths in the
    water column (to a maximum of about 350 meters), especially from sampling
    platforms with limited space.

4.  Electrical interference and vibration problems are hazards when using
    water quality monitoring systems in aircraft.

5.  Outfitting an aircraft for water sampling requires detailed planning and
    engineering.

6.  Dissolved Oxygen and  pH sensors are extremely fragile, easily fouled, and
    vulnerable to electrical interference and "ground-loops."  These sensors
    and circuitry require constant attention by qualified technical personnel
    to ensure reliable dissolved oxygen and pH data.

7.  To ensure continued,  reliable operation in the field, water quality
    monitoring systems require frequent maintenance and calibration.

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                                RECOMMENDATIONS
GENERAL RECOMMENDATIONS

1.  Helicopters are recommended for use as  water sampling  platforms  when
    large numbers of lakes or lakes located in inaccessible  areas  must be
    sampled quickly with few personnel.

2.  This helicopter-borne water monitoring  system is  recommended when  there
    is sufficient space in the aircraft and sufficient  electrical  power
    available.

3.  When electronic water quality monitoring systems  are used,  a well
    experienced electronics technician with a high mechanical  aptitude and a
    general  chemistry background should be  part of the  field team.


SPECIFIC RECOMMENDATIONS

    These recommendations may not be appropriate for  all systems;  they are
based on our experiences with our system.   They were  implemented and tested on
our system or will  be implemented on the next generation system.

1.  Because of their maneuverability on the water and ability  to take-off and
    land in a limited area, helicopters are recommended over fixed-wing
    aircraft as water sampling platforms.

2.  Static inverters are recommended where  117 V a.c. power  is  required
    (the power factor should be corrected to one).

3.  Aircraft power should be used vnthout conversion  wherever  possible.

4.  Equipment racks should be designed to conform to  the internal  contours of
    the craft.

5.  Sample storage racks should be designed, where possible, to utilize space
    that is otherwise unusable.

6.  All equipment and storage racks should  be secured to the aircraft.

7.  All permanently mounted hardware should be shock-mounted.   Shock mounts
    should conform to military specification Mil. Std.  - 810.   All deploy-
    able systems should have cushioned restraining cradles for  on-board
    storage.  All fragile electrodes should be individually  shock  mounted.

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8.  Hardware for in-situ measurements and sampling should be as  light-weight
    and compact as possible.

9.  Strong, light-weight, noncorrosive materials (i.e.,  plastics)  should  be
    used wherever possible, especially on deployable hardware.

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                       WATER QUALITY SAMPLING PLATFORMS


SAMPLING PLATFORM REQUIREMENTS AMD OPTIONS

     The NES sampling program required visits to several  hundred  lakes
scattered across the 48 conterminous United States.   Even with optimal
planning of the sampling schedule, distances as  great as  160  km (100 miles)
separated consecutive lakes.  Each lake was to be sampled a minimum  of  three
times and, generally, at more than one sampling  site.  In-situ measurements
and water samples were to be collected from various  depths in the water column
for physical, chemical and biological  analyses.   The magnitude of the program
required that the sampling platform be a)  large  enough to safely  ferry  the
sampling crew and the sampling system; b)  stable and easy to  work from  on the
water; c) capable of providing sufficient  electrical power to operate the
sampling system; d)  capable of maintaining position  at the site during
sampling operations in wind and water currents;  e) capable of sampling  an
average of eight sites per day in a 100-mile radius; f) reliable; and g) cost-
effective.

     The options for sampling platforms considered included small boats,
fixed-wing aircraft, and helicopters.   To  meet the time constraints,  use of
small boats would require a large number of independent field crews  and
monitoring/sampling systems.  This would be an ineffective use of manpower and
too expensive.  Aircraft represented a much more attractive option.

     The selection of the type of aircraft was based on several considera-
tions.  Although fixed-wing craft are  more economical  to  operate  and  maintain
than helicopters, and have a greater operational  radius in terms  of  time and
distance, they require more open water for take-off  and landing,  and  their
maneuverability on the water is minimal.  Also,  the  doors on  fixed-wing craft
are too small for convenient deployment and recovery of sampling  packages and
the standard floats used are not configured to accommodate personnel  during
sampling operations.  Helicopters, although less economical to operate  and
more limited in operational radius, can take-off and land with minimal  open
area and are capable of lateral  as well  as fore  and  aft maneuvers, and
rotation about a central vertical axis while on  the  water.  Floats are
available that have a flat, deck-like  top  surface for working personnel.  Some
aircraft have large sliding doors convenient for the ingress  and  egress of
sampling equipment.   The more reliable helicopters are powered by jet
turbines.  However,  the number of small  airports that can supply  jet  fuel,
particularly among those in rural or remote areas, are limited.   If  a
turbine-powered craft is selected, fuel  may have to  be trucked into  the
sampling area, or some intermediate location to  minimize  the  time lost  in
refueling.

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         Bell UH-1H  "Huey"  helicopters and one de Havilland U-la "Otter"
fixed-wing aircraft  were  borrowed from the U.S. Department of Army.  All three
craft were outfitted with floats and used in the field during the first month
of the NES. Because  it  required too much open water for take-offs and could
not maneuver on the  water to maintain station, the "Otter" was used only for
cargo and personnel  transport.  The "Huey" was quite maneuverable and
versatile and, after the  first month of sampling, was chosen to complete the
survey.  A third "Huey" was obtained from the Army.


HELICOPTER SAMPLING  PLATFORM

     The three Bell  UH-1H "Huey" helicopters (Figure 1) used as sampling
platforms are thirteen-passenger craft with a total payload of 1,400 kg (3,100
Ibs) at sea level, and  are  powered by a single jet turbine delivering 320-kw
(mechanical) (1,100-shaft horsepower).  The total available electrical  power
to operate sampling  equipment is approximately 200 A at 28 V d.c. or 5,600
watts.  Each aircraft was fitted with floats (a standard Bell  retrofit kit) to
allow it to land on  the lake surface (as opposed to hovering)  thereby
conserving fuel, reducing the risk of injury, and providing greater sampling
convenience.

     The helicopter's main  fuel tank capacity is 635 kg (1,400 Ibs), yielding
about 2 hours of flying time at 100 knots (the airspeed is limited to 100
knots by the floats).   After the first year of the NES, an auxiliary fuel tank
with a capacity of 136  kg (300 Ibs) was installed in each aircraft.   This
increased the flying time by 1/2 hour.  While performing sampling on the water
surface, the turbine was  idled to reduce fuel consumption (the rate at flight
idle is about 1/2 the flying consumption rate) and yet retain sufficient power
to maintain position on the station.  The total  time between refueling stops
was in excess of 2-1/2  hours since each half hour of sampling consumed the
equivalent of only 15 flying-minutes of fuel.

     All of the seats except those of the pilot and co-pilot were removed to
accommodate the auxiliary fuel tank, sampling equipment and sample  storage.
The auxiliary tank served as a bench seat for the sampling technician and any
additional passengers.  The arrangement of the sampling equipment and storage
is shown in the floor plan of the cabin (Figure 2).

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                                                     ^.At^ •'^•ffn-fH-vt          ™^M%i,, " J3»-'         .T



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   /Figure 1   In-Xitux^ensor^ump Assembly Being Deployed  from the "Huey" ^felicopter ^hile^On Ration.
     ^

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Figure 2.  Floor plan of the helicopter with  the  monitoring/sampling  system  located,

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                    HELICOPTER-BORNE WATER SAMPLING SYSTEM


     Each of the three Hueys was outfitted with a sampling system.  The
sampling system consists of six in-situ sensors (Table 1), their associated
analog electronics, and a submerged pump, mounted together on a frame; a hose/
cable, boom assembly and winch; on-board electronics; a solar illuminance
meter, a Secchi disk and a sediment sampler (Figure 3).


     TABLE 1.  THE PARAMETERS AMD TYPES OF SENSORS USED ON THE INTEROCEAN
                             IN-SITU SENSOR SYSTEM


             Parameter                      Type of Sensor


          Conductivity                Inductive sensor

          Temperature                 Glass-bead thermistor

          Depth                       Wheatstone bridge
                                       strain gauge
                                        pressure transducer

          Dissolved Oxygen            Polarographic membrane
                                       w/external  temperature
                                        compensation

          pH                          Mon-ruggedized combination
                                       pH electrode w/external  temperature
                                        compensation

          Turbidity                   Optical  transmissometer
IN-SITU SENSORS AND PUMP

Assembly

     The in-situ sensor/pump sampling system was of a commercial  ship-board
design for use in a marine environment and manufactured by InterOcean of San
Diego, California (Model 500, modified to include a pump sampling system).  It

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POWER lllllilltiE»9191
WATER
SIGNAL
          Figure  3.   Block diagram of the helicopter water  quality
                         monitoring/sampling  system.
                                       10

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was heavier and bulkier than necessary  for  the  acquisition of data from most
inland water bodies.   Both  mass  and  bulk  are critical in airborne applica-
tions.  The in-situ sensor  assembly  consists of five sensors mounted on the
end of a cylindrical  stainless steel  pressure case  (10 cm diameter, 41.3 cm
length, 8.5 kg weight) and  a sixth optical  sensor mounted on the side of the
pressure case.  The case housed  the  analog  electronics and calibration
controls for the sensors.   The pressure case and pump were mounted on a
stainless steel  frame 30 cm (12  in)  in  diameter and 96 cm (38 in) in length.
The frame was mechanically  attached  to  the  hose/cable via a Kellem"" grip.  The
signal and power cables, and the hose "fanned-out" from a potted splice on the
end of the hose/cable.

     Handling of the sensor package  during  sampling operations was initially
the source of some sensor damage.  In particular, the dissolved oxygen sensor
and the pH sensor were occasionally  broken  and  the alignment of the
transmissometer optics was  disturbed.  Reduction of the size and weight of the
probe-assembly and relocation of the turbidity  sensor in a more protected
location, closer to the center of the assembly,  reduced the vulnerability of
the sensor to rough handling (Figure 4).
                                  Sensor
                                  Pressure
                                    Case
      Original  \
     Location
     Turbidity   J



  Final
Location
Turbidity
                                   Pump
                                     &
                                   Motor
  Figure 4.  Plan view of the  in-situ  sensor/pump assembly showing relocation
                            of the  turbidity  sensor.
                                     11

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 Junction Box

      Originally, there was no provision for strain relief  where  the pigtails
 to  individual  components on the sensor/pump assembly  exited the  potted splice.
 The constant motion of the wires at the splice caused some conductors to fail.
 Repair was  not readily effected since the potting  compound had to be cut away,
 the pigtails respliced to very short wires at the  pot,  and the splice resealed
 to  maintain watertight integrity.  InterOcean provided  the solution to this
 problem.  The multiconductor cable/hose was brought into a watertight oil-
 filled junction box.   The box was fitted with rubber-bushed plastic stuffing-
 glands for  cable entry and a rubber diaphragm in one  side  for pressure
 equalization.   All  splices are now inside the junction  box and are easily
 repaired or modified.  The box is mounted firmly to the frame so the cables
 can no longer  move  and be broken (Figure 5).

 Pump

      The pump  originally supplied by InterOcean was a 250-watt (mechanical)
 (1/3 horsepower) 11-stage centrifugal  well  pump of cast naval bronze.  It
 weighed 16  kg  (35 Ibs) and was 80 cm (32 in)  in length  and 9.5 cm (3 3/4 in)
 in  diameter.  The motor was manufactured by Franklin  Electric Company.  The
 pump delivered about  7.5 1pm (2 gpm) through  a 100-meter length of hose/
 cable.   The delivery  rate is inversely proportional to  the total length of
 hose;  it  is  not affected by sampling depth.  There was  a protective screen
 over the intake that  would pass nothing larger than approximately 1.6 mm
 (1/16  in) in diameter.  The screen  blocked  the passage  of  algal  masses and
 jeopardized quantitative estimates  of chlorophyll  a_ and phytoplankton
 components.  When the screen was removed,  accumulated algae eventually clogged
 the pump  impellers  and stopped the  pump.   The length  of the pump was the
 limiting  factor in  reducing the size of the sensor/pump assembly.

      Since  a smaller, lighter,  positive-displacement  pump  would  allow
 reduction in the size and weight of the assembly and  avoid the clogging
 problems, such a pump, the Hypro Flexroller model  B-612, which weighs  only 2
 pounds  without the  motor,  was procured.   It required  very  low start-up torque,
 and  was  designed for  a maximum  operating  speed of  1,800 rpm.   The pumping seal
 was  maintained by four rubber rollers  (Figure 6).   The only sealed submersible
 motor  available that  met  size,  mechanical,  and electrical   power  requirements
 was  the motor  from  the original  pump,  which ran at 3,400 rpm.   The rubber
 rollers  in  the pump were  replaced with  Teflon rollers, and the pump and  motor
 combination  was tested with the hose/cable.

     The  new pump,  operating at 3,400  rpm,  delivered  more  than 34 1pm  (9 gpm)
 at  zero meters  of head.   However, when  34  1pm of water was forced through 100
 m (328  ft)  of  9.52-mm (3/8-in)  tubing,  the  friction head loss in the tubing
 was  greater  than 300  m (1,000 ft).   In  other  terms, the pressure differential
 from one end of the hose  to the other  was  about 30 kg/cm*  (430 psi).  This  was
more pressure  than the hose  could withstand and required more  power than a
 250-watt  (1/3-hp) motor could deliver.  The solution was to relieve the
 pressure and reduce the  flow accordingly.   A  "bleed-hole"  was  drilled  in the
                                     12

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                Sheave
                                                                           Junction
                                  Pressure Case r

    \ .1- - "• ••»•••'
    \ ' ~ - «; -i.«i
                                                                         . Flexible Coupling
Figure 5  In^itu Censor/Pump Assembly with Components..Identified.

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                                                                                                            BKTH
-A--.
  Figure 6.  Hypro model  B-612  positive displacement pump shown on the'left  1s  Identical  intern-ally to the
             model B-634  shown  in cut-away on the right,

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output of the pump and was enlarged  until  the  pressure  at  the  pump was  reduced
to 7 kg/cm2 (100 psi).  This  diverted  3/4  of the  pumped water  back to the  lake
and yielded a flow rate of about 7.5 liters  (2 gal)  per minute which was
acceptable for sampling.  A better solution  might have  been  a  slower motor
(1,800 rpm) or a gear reducer between  the  motor and  the pump.   Neither  was
readily available in a compact watertight  assembly and  the method used
represented a simple, cost-effective solution  to  the problem.

     After modification the smaller, lighter pump assembly (Figure 5) weighed
8.6 kg (19 Ibs) and v/as 46 cm (18 in)  long vs. the 16 kg  (35 Ibs) and 31 cm
(32 in) of the original assembly (both weights include  motor).  Thus a  new,
smaller frame assembly, 30 cm (12 in)  in diameter by 81 cm (32 in) in length,
was fabricated from aluminum  (Figure 5), which provided a  significant
reduction in weight and bulk.  The frame could have  been made  smaller but
these dimensions provided some sensor  protection  and facilitated handling
during deployment and retrieval.

Sensor and Analog Electronics

Conduct!vity--
     The conductivity sensor used is an inductive type, designed and
fabricated by InterOcean that consists of  two  torroidal coils  molded together
in a donut shape (Figure 7).   The pair of  coils acts as a  transformer.   The
electromagnetic coupling between the coils is  a function  of  the conductivity
of the water column that passes through the "donut."  One  coil is driven with
a "saw tooth signal" generated by an oscillator;  the amplitude of the signal
in the second (sensor) coil is processed  to yield a  d.c.  voltage analog of
conductivity.  This type of sensor is  not  susceptible to  electrode
contamination.  Only one problem was experienced  with this conductivity-
measuring device; the driver oscillator frequently would  not initiate
oscillation at temperatures below 0°C.  The value of the  resistor that
provided the forward bias to the oscillator transistors was  changed  to  move
the operating region to a more reliable range  on the "characteristic  curve"  of
the transistors.

Temperature--
     The response time of the InterOcean glass bead-thermistor temperature
sensor was too long to allow rapid profiling of the  water column.  The
thermistor and its electronics were replaced by a platinum resistance
thermometer (originally manufactured by Rosemount and adapted  by InterOcean)
and appropriate analog electronics  (InterOcean retrofit).  The time  constant
for the thermometer is 60 milliseconds, more than a  factor of  50 faster than
the thermistor, and thereby allowed much more rapid  temperature profiling.
The platinum resistance thermometer sensor cost $485.00 each vs. $4.00  for the
thermistor at the time of purchase  in 1972.

Depth--
     The depth sensor  is a bonded strain-gauge pressure transducer;  the
measuring  element is  a  resistive Wheatstone bridge.   Some units were
temperature sensitive, and changed  calibration with  a temperature change.
Some sensors exhibited as much as 1/2 foot per degree Celsius  drift.   Those
sensors with extreme drift were replaced by the manufacturer.   As a  backup

                                     15

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iaure 7. :' &
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                                                                   s

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means of obtaining measurements of the depth of sampling,  the  hose/cable was
marked at 5-foot intervals with color-coded tape.   Visual  interpolation was
employed when the depth value was located between  the markings.

Dissolved Oxygen (DO)--
     The response times of the dissolved oxygen sensor (Beckman  39552)  and
associated temperature-compensation thermistor were too long (in excess of 5
seconds) to allow rapid profiling.  The change in  the sensor and circuitry for
temperature allowed and improved dissolved oxygen  sensor response time.  By
utilizing the analog output voltage of the temperature circuitry rather than
the thermistors to provide temperature compensation information  for the oxygen
circuitry, the response time of the dissolved oxygen information was reduced
to about 4 seconds (Appendix A).  The oxygen sensor provided by  InterOcean had
two advantages over other sensor designs:  1) the  low volume and low thermal
mass yielded quicker responses to temperature and  oxygen changes than other
oxygen sensors, and 2) the low oxygen-consumption  rate eliminated the need for
a stirring mechanism.  However, the sensor was very fragile and  developed
internal electrolyte leaks that yielded inflated oxygen readings.  Vie were
forced to change to a more rugged sensor, manufactured by Yellow Springs
Instrument Company, which had a slower thermal response.  The  oxygen sensor
provided by Yellow Springs (YSI) is model YSI 5419, a pressure compensated
sensor that YSI installed in a stainless steel fitting provided  by EPA (Figure
8).  The sensor has a body of PVC and acrylic, and silver and gold electrodes;
it is a clark-type polarographic electrode.  InterOcean has since gone to yet
another supplier for DO sensors.

     There are still inherent problems with the new DO sensor.  The sensor
membrane is easily torn and subject to fouling by  algae and sediment.  The DO
circuitry is vulnerable to electrical interference and "ground-!oops;" the
polarizing potential applied to the sensor can, at the same time, interfere
with the pH signal.  Both sensor and circuitry require frequent  attention by
the technician.  For these reasons, we found this  sensor to have limited
utility in the helicopter application.

pH-
     The combination electrode for pH was the most fragile of all the sensors.
InterOcean redesigned the sensor mounting to  improve its resistance to shock,
but the electrode still failed mechanically in our application.   Failures were
attributable to both extreme shock (such as dropping the sensor package) and
to aircraft vibration.

     The circuitry provided by InterOcean for the pH analog was  quite
vulnerable to electrical  interferences and ground loops from other sensors and
the aircraft.  We redesigned this circuit utilizing a differential amplifier
(Appendix B), and separate Beckman Lazaran reference and glass measuring
process-type electrodes (Figure 9) that resulted in a much more  rugged sensor
assembly and more stable, interference-free electronics.  InterOcean now uses
a similar differential amplifier circuit to reduce interference.

     The modified circuit of the extremely high-input-impedence amplifier
required by pH sensors (>1012 ohms)  is less vulnerable to "ground-loops" and
electrical interference.  Very clean water (specific conductance <200

                                     17

-------
                                Stainless Steel Fitting (Screws into Pressure Case)
                                                               Pressure Equilizer
                \
                                                                            'O' Ring
                                                                            Membrane
                                                                            Retainer
                                                                           Membrane
          Protective Cap
Figure,8  Dissolved
How Springs Instruments).

-------
                Stainless Steel Fitting (Screws into Pressure Case)
                                                                          Beckman
Stainless Steel Electrode Housing
Containing Buffer Preamp
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-------
ymhos/cm) does not ionize  sufficiently for the p'H sensor to be effective.
When the sensor package  is not in the water, a protective cover filled with
water should be placed over the glass measuring electrode to keep the
electrode v/et. If dry, the sensor may have to be soaked for 2 to 3 hours
before consistent pH values can be obtained on calibration.

Turbidity--
     The turbidity sensor used, is a simple transmissometer consisting of a
light bulb, a photo cell, and associated optical system.  The output signal
is an analog of the percentage of light transmitted along a fixed 10-cni path
length through the water.  This is displayed on the digital display.  The
analog meter is labeled  in formazine turbidity units (FTU), however, the FTU
values do not correlate  with Jackson Turbidity Units (JTU) in all cases.  The
only problems with this  sensor were those of mechanical misalignment of the
optics caused by rough handling and its relative lack of precision.


HOSE/CABLE, BOOM, SHEAVE AND WINCH

     The in-situ package mechanical delivery/recovery system that consisted
of a hose/cable, boom, sheave and winch from InterOcean provided the means to
recover water samples from discrete depths, supply electrical power for the
sensors and pump, and transmit the analog signals (Figure 3).

Hose/Cable

     The hose/cable, a concentric structure, was constructed with a 10 mm
(3/8 in) i.d. nylon sample delivery hose at the center.  Twenty conductors of
20 gauge stranded, Teflon  insulated wire were wound around the hose at about
one turn per 60 cm (2 ft), and the voids between the conductors were filled
with a gelatinous substance to minimize water movement in this layer if the
cable inner jacket were  damaged.  These power and signal transfer conductors
were covered with a single, concentric vinyl jacket, and a galvanized steel
wire strain member was braided over the jacket to support the mechanical load
of the in-situ sensor/sampler system.  The voids in the braid were not filled,
and the braid was covered with a second vinyl outer jacket about 0.305 mm
(0.012 in) in thickness.

     In use, this thin vinyl outer jacket was easily damaged and the braid
exposed.  Water from the outside then moved along the strain-member layer.
The natural working of the braid strands against each other wore away the
galvanized coating, which  allowed the steel braid to rust and ultimately led
to strain-member failure.  Fortunately, the conductors and the hose were
capable of supporting the  relatively light load of the sensor package.  When
the cable-design oversight was detected, we specified and procured new cable
with a stainless steel braid instead of the original galvanized steel and a
much thicker (2.4-mm) outer jacket.  We also specified a smaller bend radius
(20.3 cm (8 in) vs. 35.5 cm  (14 in)) than that of the original cable, in order
to reduce  the diameter of  the sheave on the boom to 40.6 cm  (16 in).  The
original hose/cables were  each 100 meters (328 ft) long.  The new cables were
100 and 200 meters long  to fill the new winches.

                                     20

-------
Boom

     The original boom was mounted on a pivoting mast that attached to
existing fittings in the deck and the overhead in the craft.  After the first
year, a new hinged-boom system was designed which attached to three existing
attachment points in the "overhead" and used a fourth point for bracing in the
extended mode (Figure 10).  This configuration allowed relocation of the
winch a few centimeters forward and outboard of its original position, which
in turn, made available the necessary room to add an auxiliary fuel tank.

Sheave

     The sheave provided on the original procurement was a massive aluminum
casting more appropriate for marine applications.  For our relatively short
cable and cramped quarters onboard the aircraft, a smaller, lighter sheave was
needed.  This need was satisfied by casting polyurethane in the modified rim
of a heavily constructed 40.6-cm (16-in) bicycle wheel and then machining a
groove in the urethane that fit the cable radius (Figure 11).

Wi nch

     The winch provided the mechanical recovery and hose/cable spooling
functions, and provided for continuous signal and power transfer through slip
rings.  A rotary union permitted continuous water sample transfer when the
winch was in motion.

     The original winch provided by InterOcean was powered by & variable
speed, direct current control motor through a planetary gear speed reducer
and a chain drive to the drum.  The winch controllar converted alternating
current from the inverter to variable-direct current for speed control of the
drum.  The hose/cable was secured to the drum with a Kellem grip, and the hose
member was attached to a rotary union in one end of the winch drum.  A
20-conductor slip ring assembly extended outside the winch.  The conductors
of the cable were hard-spliced to the slip rings inside the winch drum.
Changing slip rings in this configuration was a major task.  In general, the
winch was too large to work around conveniently and was inadequately braced.
It also had no provision for manual operation in the event of an electrical
power failure, a serious safety consideration.

     Between the first and second field years a new winch was designed, and
three copies were fabricated (Figure 12) that overcame the operational
shortcomings of the original winch.  Two of the new winches had 100-meter
capacity drums and one had a 200-meter drum.  The slip rings v/ere  recessed
into the winch drum and a connector was added between the rings and cable;
this connector was accessed through a service port in the side of the drum.
This winch was powered by a control motor similar to the original winch, but a
worm gear speed-reducer was used.  A manual crank (Figure 12) was  included to
retrieve the cable in the event of a power failure and a manual brake was
added to the drum to prevent free-wheel ing in the event of a broken drive
chain.  A slipclutch was incorporated in the drive assembly to prevent damage
to the air frame at the boom attachment points  if the sensor/sampler were


                                     21

-------
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     Removeable Emergency Hand Crank



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Figure 12 New 100-meter

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 accidentally  snagged  on  some  submerged object.   The  chief advantages  gained
 from the  redesign  of  the  boom and winch assembly were  in operational  safety
 and size  reduction.   The  latter allowed relocation in  the aircraft; the  gain
 in working space made it  possible to replace the rear  seat with an auxiliary
 fuel tank mounted  athwart  ship.  The tank doubled as a seat and extended  the
 operational radius of the  helicopter.
ONBOARD ELECTRONICS

     The onboard electronics  included the console  for the in-situ sensor
subsystem, the pump and winch controls, the graphical displays, the echo
sounder display and the power supplies (Figure 13).

Mounting Rack

     An open-frame 19-inch relay rack was fabricated from aliniimim angle-
stock.  The base was 20.5 inches (52 cm) square and was mounted on shock
mounts specially designed for helicopter use.  The overall height of the rack
and mounts was 52 inches (132 cm).  The back of the rack was shaped to fit the
contours of ttie helicopter cabin, and the rack was bolted to the deck of the
helicopter.  The onboard electronics systems were installed with the static
inverter, the heaviest component (82 kg, 130 Ibs), at the bottom; then the
pump and winch controllers and the in-situ sensor console; and finally the
four graphical recorders at the top (Figure 13).  The echo sounder display
was mounted on the side of the "relay rack."

Console

     The console and digital-display module for the InterOcean model 514A was
provided in a box for portability.  This was remounted in the relay rack to
consolidate the onboard electronic systems.  The console operates on 12-vclts
d.c. power and provides the power to the analog circuitry for the sensors.  It
also contains the analog to digital  conversion circuitry, analog panel  meters,
and a switchable digital  display for greater resolution of the measurements.
This was the single most trouble-free component in the entire  system.  The
only change made in this component was a new front panel provided by
InterOcean to fit a 19-inch relay rack.

Graphical  Displays

     The original  graphical  recorder was a x,y,y', y" flatbed recorder with  a
plotting area 27.9  cm (11 in)  by 43 crn (17 in).  Neither the electronics  nor
the mechanical pencarriage assembly could withstand rotary-winged aircraft
vibration.  Four separate strip chart recorders,  Hewlett-Packard model  680A,
were substituted.  An event marker feature was used to key regular depth
intervals  to correlate the values noted to specific depths.   Electric-writing
recorders  were selected to avoid the inevitable untidiness of filling ink
reservoirs in an aircraft.
                                     25

-------
                                                                m^Styff'fi'iv-^^'i-^i':.' '"..• •'"•
                                                                                                                    Strip Chart
                                                                                                                    Recorders(4)

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                                                                                                                               eadout
                                                                                                                             Console
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Winch Controller

     The winch controller, Browning model M, was modified mechanically to
mount behind a relay-rack panel.  The controller is powered by 117-volt
alternating current and provides a variable d.c. voltage to the winch notor
for speed and direction control.

Pump Controller

     The pump controller includes the main power switch for the pump, the
"power factor" correction capacitor, a step-up transformer (117 V a.c. to 230
V a.c.) to pov/er the motor from a 117-V a.c. source, the starting-winding
capacitor, and starting-winding disconnect relay.  The transformer and
correction capacitor are mounted on a panel  on the rear side of the rack.  The
remaining components comprise the standard "1/3 horse power" Berkley pump
controller and are mounted on the same panel as the winch controller.

Power Supplies

     The power available on the aircraft is 23 V d.c.  with maximum of 200
amperes available.  Most of the sampling and measuring equipment requires 117
V a.c. power.  During the first year of the NES, a 1,000-VA rotary inverter,
Leland Model MGE 37-400, was used to convert the 28 V d.c. to 117 V a.c.  60
Hertz.  Rotary inverters function better than static inverters when used  with
inductive loads, e.g., the pump motor; however, they are inefficient when
little current is being drawn from them.  One thousand volt-amperes was just
sufficient to operate the winch and pump simultaneously.  When the pump
clogged, the motor would draw more current than the inverter could deliver.
This repeated overloading of the inverter caused numerous inverter failures.
Larger capacity rotary inverters ware not available.  After the first year of
NES, the rotary inverter was replaced with a 2,000-VA static inverter, Um'tron
model  PS 67-324-1.  The inductive load of the pump motor was corrected to a
power factor of about one by adding an appropriately sized capacitor in
parallel with the load.  The winch motor required no correction since the
power supplied to the inductive load by the  winch controller was d.c.  The
static inverter is much more efficient than  the rotary inverter,  especially
during low load conditions.  However, it weighs 54 kg  (120 Ibs) more than the
rotary inverter.

     The static inverter provided 117 V a.c. power to  operate the pump, the
winch, and four strip chart recorders.  The  echo sounder and the console  for
the in-situ sensors required 12-volt d.c. power.  A Narco 28'  to 12-V d.c.
power converter was used to power these components.  The converter was
mounted on the back panel  of the rack next to the pump step-up transformer.

Echo Sounder

     A number of different pleasure-boat echo sounders of the revolving-
disk/flashing-light type have been installed on the helicopter since 1972.
Some of the manufacturers  and models are as  follows:
                                     27

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        Columbian Hydrosonics    Model  C8-324      Ray Jefferson     Model  520
        Heath Company            Model  MI-1031

 However,  none was found to be rugged enough to endure constant use  and
 helicopter vibration.  The bearings supporting the revolving  disk and motor
 armature  typically failed after a few months of field use  and replacement
 parts  were not readily available.

     The  indicator unit was mounted on the front of the  winch/pump  controller
 panel.  The transducer is addressed in "Other Underwater Components."


 SAMPLING  MANIFOLD AMD SAMPLE STORAGE

     Sample water delivered from the rotary-union on  the winch was  transferred
 to  a cylindrical  clear-acrylic  manifold  for sample delivery.  The manifold was
 51  mm  (2  in)  diameter, 305 mm (12 in)  long and was oriented horizontally.
 Three  sample  ports on the bottom were  fitted with plastic  valves for sample
 withdrawal.  The supp-ly hose from the  winch was introduced at one end and an
 overflow  hose was attached to the other  end.  A small  stainless steel sink 102
 mm  (4  in)  by  205 mm (12 in) was mounted  below the manifold.  The sink drain
 and maniFold-overflow hoses drained outside of the helicopter.  Both the
 manifold  and  the sink were mounted on  the  side of a specially fabricated
 aluminum  sample-storage cabinet.

     Samples  were collected in  125-ml  (4-oz) polyethylene bottles (flalge
 £2003-0004) which were carried  in plastic  coated wire  bottle racks, 48 bottles
 per rack  (Cole Farmer #6049-10).   The  sample-storage  cabinet was constructed
 such( that  three racks of bottles  fit on  each of four  shelves.  A large space
 at  the bottom of  the  cabinet was  reserved  for tools,  spare system parts etc.


 OTHER UNDERWATER  COMPONENTS

 Solar  Illuminance Meter and Secchi  Disk

     At the outset of NES,  secchi  disk measurements represented the sole basis
 for estimating photic zone  depth.   Beginning in the second year,  a  solar
 illuminance meter was used  in addition to  the secchi disk.  A Montadero-
 Whitney model  LMT-8a  was  chosen.   It functioned well during the remaining
years of NES  and  on other later applications.   This instrument was deployed
 independently  of  the  sensor/pump  system.   A  possible  improvement  would be to
 integrate  it  with the sensor/pump  assembly.   This  would  have required that
only one system be put  in  the water and  would  have expedited the  on-site
operation.  Both  the  secchi  disk  and the solar illuminance meter readings,
when taken  from the helicopter, were shallower  than when taken from a boat at
the same site.  This  is,  in part,  attributed  to  reflections and refractions
at the v/ater  surface  from the rotor-wash turbulence.   In the case of the
secchi  disk,  the  demonstrated shading effect  of  the rotor blades  also
contributes to  the shallower readings.  Generally, the 1-percent  light level,
as measured from  the  helicopter float with  the  light meter, was about 2.2
times the secchi  disk  depth  measured from  th-a  same position.

                                     28

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Sediment Sampler

     Bottom sediments were collected for nutrient and heavy-metals analyses.
The original sampler was a Phleger corer.  The intent was to describe the
stratification of the nutrient accumulation in the sediments.  The corer
resembles a bomb, with a core tube and cutter on its anterior.  This assembly
is dropped into the water and rapidly decends to penetrate the bottom.  When
it is extracted it recovers a 35-mm (1.375-in) diameter cylinder of sediment,
essentially undisturbed.

     The corer was too heavy to recover by hand and interferred with the
primary mission of collecting water samples.  Recovery after complete
penetration into the sediments sometimes resulted in failure to the boom
system.  The concept of collecting intact cores was waived for expediency and
a small, light-weight clam-shell dredge, which could be deployed and recovered
by hand with a 3-mm (1/8-in) nylon line, was used for the remainder of the
study.

Echo Sounder Transducer

     The echo-sounder transducer was mounted on a fixed bracket on the left
helicopter float.  The transducer was positioned such that when the aircraft
was on the ground the transducer was about 20 cm (8 in) above the ground and
when the aircraft was on the water the transducer was submerged about 5 cm (2
in).

     When different manufacturers'  echo sounders were used, the transducer
was also changed to ensure compatibility.
                                     29

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                               SAMPLING OPERATIONS


      As a safety precaution, sampling should never be  conducted with fewer
 than three persons.  Thejjilot, riding in the right-hand  front seat, is
 constrained to remain seated and to maintain control of the aircraft at all
 times,  in the air, on the water, and on land, as  long  as  the main rotor is in
 motion.  If someone sampling has an accident or falls  overboard, the pilot is
 not at  liberty to leave his place to lend assistance.  The limnologist rides
 in  the  left front seat and identifies the sampling sites.  The sampling
 technician rides in the rear of the aircraft.  The limnoTogist records
 observations  concerning the site location,  general  water  appearance, and any
 other observations pertinent to the mission on a  field data sheet.  After
 the sampling  site is  reached and the aircraft has  landed  on the water, the
 technician debarks to the right float and deploys  a  station reference buoy for
 the pilot.  While on  the water with the helicopter at  flight idle for
 sampling,  the pilot maintains position on the site against wind and/or
 currents.

     _The  limnologist, meanwhile, debarks  to the left float and deploys the
 in-situ sensor/sampler package.   The limnologist then  occupies the techni-
 cian's  seat  at  the system console  to operate the  winch and pump during the
  cast"  (Figure 3).   The technician  takes  secchi disk observations and
 bucket-dipped  surface-water samples.   The limnologist lowers the sensor/pump
 package slowly through the water column until  it contacts the bottom {or the
 cable end  is  approached).   During this  process, the four analog strip-chart
 recorders  are  activated to generate analog  profiles of conductivity,
 temperature,  dissolved oxygen and turbidity.   The  sensor/pump is  then raised
 free  of the bottom  before  the pump  is activated, to avoid damage to the pump
 from  sediments entering the intake.   Water  samples are collected  and preserved
 as  necessary  by  the technician.   Samples  are  collected from a manifold inside
 the aircraft.  Sufficient  time must  elapse  after starting the pump for  the
 hose/cable to  be  purged of water from the previous sampling (purge times  were
 routinely  measured  for each system).  Sampling depths for the collection  of
 other water samples are chosen by the limnologist  after inspection of the
 analog  profiles of  the four parameters.   The  digital display on the  console is
 read and recorded on  the field data  sheets  for all of the parameters at each
 sampling depth.   Upon  completion of sampling  near the bottom,  the  sensor  is
 raised  to  the  next  level,  digital values  are  recorded,  the hose is  purged  and
water samples are pumped.   This  process is repeated at  each depth  selected for
collection  of water samples at a given  site.

     Integrated samples  for some analyses are collected by continuing to  pump
while raising or  lowering  the sensor  package.  Water collection is timed   to
provide a  uniform mixture  of  water over the integration depth  range.


                                      30

-------
     Samples are collected in polyethylene bottles for all  required  types of
analyses, both chemical  and biological,  are preserved  as  necessary,  and
stored in racks in the sample storage cabinet or in an ice-chest  as  sample
requirements dictate.

     When sampling in rivers with noticeable current,  as  was  sometimes the
case in both NES and the Atc'nafalaya Study, the sensor/pump package  is not
lowered through the water column, but rather is used for  surface  samples  and
measurements only.

     At the conclusion of sampling,  the  limnologist retrieves the sensor/
sampler from the water,  secures it in the aircraft and returns  to the left
front seat before take-off.  The technician recovers the  buoy and returns to
his seat for take-off.  Because of the high noise  levels, headsets connected
to the helicopter's intercom system are  used for communications while sampling
as well  as during flight.
                                    31

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                           CALIBRATION AND MAINTENANCE


      The sensor analog electronics proved to be  quite  stable; however, the
 wet/dry cycling of the sensors and the physical  abuse  from deployment/
 recovery and sensor contamination necessitate daily calibration.  During
 routine operations the system is typically calibrated  to  standards and buffers
 in  the  evening {Table 2)  and checked against these standards the following
 morning before flight.  Differences between the  system and standards for the
 morning check are recorded and added/subtracted  from the  observations made
 that  day.   The operational checks (Table 3)  are  performed both evening and
 morning concurrent with calibration and  calibration check.

      Before beginning the day's  operation the sampling technician reviews a
 check-list  for the presence of sampling  supplies:  bottles, reagents, ice and
 other preservatives;  removable sampling  gear (sampling bucket, secchi disk
 sediment sampler and  tool kit);  and spare parts  (pump, pump rollers and pump
 motor).

      Maintenance that was accomplished outside of this daily schedule was in
 response to catastrophic  failures.   In order to  ensure reliable calibrations
 and timely  maintenance,  it is  necessary  to  keep  a calibration/maintenance
 person  available to the system daily while  the system  is  in use.   This
 calibration/maintenance person should be  a  qualified electronics  technician
 with  an  understanding of  water chemistry  and  water quality sensors.

     A  portable  artificial  water  column  for  calibration of the echo-sounder
was devised  and  is illustrated in Appendix C.

     A buffer-board was designed to  be added to the console  to simplify
calibration.   This modification was  never fully tested or implemented;  it is
 illustrated  in Appendix D.
                                     32

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   TABLE 2.  DAILY CALIBRATION PROCEDURE FOR HELICOPTER BORNE WATER QUALITY
                              MONITORING PACKAGE
Parameter or Circuit
                Procedure
Bipolar regulated power
  supply
Ground current
  regulator
Temperature Analog
  zero
  gain
Conductivity Analog
  zero
  gain
Depth Analog
  zero
  gain
Turbidity
Using a 4 1/2-digit digital volt meter, the positive
and negative outputs of regulator are calibrated to
8.000 V d.c. ± 0.001 V d.c.
Using a 4 1/2-digit digital volt meter, the voltage
between the two white test points is checked.  The
voltage should be 0.000 V d.c. for proper function.
Using an ice water bath on the temperature sensor
with constant stirring and monitored by laboratory
thermometer, the zero pot is adjusted to yield
an electrical analog (0.100 V d.c. = 1.0°C) of the
thermometer indication.

An ambient temperature water bath monitored by a
laboratory thermometer is applied to the temperature
sensor.  The gain pot is adjusted to yield an
electrical  analog (0.100 V d.c. = 1.0°C) of the
thermometer indication.
With the conductivity sensor clean and dry, the zero
pot is adjusted to yield an electrical analog
of zero for conductivity 1.000 V d.c.  = 1,000 ymhos.

With the buffer box provided by the manufacturer
set to 2,400 vimho position and the wire looped
through the sensor, the gain pot is adjusted to yield
2,400 ymho analog or 2.400 V d.c.
With the sensor connected, but not  in  water,  the zero
pot is set to yield 0.000 V d.c.  output.

With shunt points shorted on depth  board  the  gain
pot is adjusted to yield the shunt  value  for  that
serial number transducer from calibration sheet.

The windows are cleaned, the lamp current set to
60.00 and the full scale air calibration  is set  to
96.00%; then, with the optical system  occljded with
a card, the zero is checked.           ,    ...    .*
                                       (continued)
            33

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                             TABLE 2.  (Continued)
Parameter or Circuit
                 Procedure
Dissolved oxygen
  zero
  gain
The sensor is bathed in a  stream  of dry  nitrogen or
dry helium and the zero pot is  set to yield  a  zero
analog on the sensor console.

In ambient air, the gain pot  is set to the 02
saturation value at ambient temperature  on the
sensor console.
  zero
  gain
Echo sounder
With the sensor in a pH 7  buffer,  the  zero  pot  is
adjusted to yield 7.00.

Then with the sensor pH in a  10  buffer,  the  gain pot
is adjusted to yield a pH  10.00  on the console
display.  With the sensor  in  pH  4  buffer, the gain
adjustment is checked for  pH  4 on  the  display.
                        With the sensor in a 10'x4" water column (Appendix C)
                        the speed of the motor is adjusted to yield blips at
                        exactly 10 foot intervals after the motor is
                        lubricated.  The oscillator and electronics alignment
                        are checked by seeing at least 10 blips  at 10-foot
                        intervals or to 100 ft in a 10 ft pipe.
                                     34

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TABLE 3.  DAILY OPERATIONAL CHECKS  OF  THE  IN-SITU  SENSOR/SAMPLING  PACKAGE
       Equipment
                Procedure
Strip chart recorders (4 ea)
Static Inverter (output)
Winch
Pump
With the input shorted,  the zero control  is
set to position the pens to zero on
the paper, for each recorder.

The inverter is turned-on,  the winch brake is
set, and the winch started  slowly while the
output voltage, frequency and  current are
monitored via the meters on the inverter.
The inverter should deliver 10 amps to the
winch without a voltage  or  frequency change.

After the inverter check, the  winch drive is
then checked in both the deploy and recovery
directions.  The winch drum slip clutch
tension is checked.

The sampling pump is checked for worn rollers
and impeller, worn motor and pump bearings
and hard starting of the punp  motor (This is
only accomplished at the evening check,
except for the ease of starting).
                                     35

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                              SAMPLING CAPABILITIES


      The airborne sampling system is totally integrated-,  the  equipment  has
 been custom-tailored to fit the interior contours of a UH-1H  model  "Huey"
 helicopter; thus the helicopter platform has become part  of the system.

      The helicopter has an operational  period of about 2  hours at 100 knots
 (airspeed limited by floats),  without auxiliary tanks. An accessory auxiliary
 tank can extend that period to about 3  hours.

      It  should  be noted that 3 hours times 100 knots  air  speed does not
 necessarily equal 300 nautical  miles of ground track,  because of head winds
 or  tail  winds.   Fuel  consumption is  cut in half when  the  aircraft is at flight
 idle during sampling.  Travel  time from the operations base to the first
 sampling site and the distance  (or time)  between sampling sites strongly
 influence the number of sites  that can  be covered between re fuel ings.  Factors
 of  lesser influence include the type of sampling,  the  number of samples par-
 site and the depth of water at  each  site.  Thus it is  difficult to make any
 meaningful  statement  about the  number of  sites that can be sampled in any
 given time  period.  However, during  the NES,  the sampling extremes ranged from
 one  site per refueling  (far away from the base)  to nine sites per refueling
 when the sites  were close to one another, and to the  operations base and the
 sites  were  shallow (less  than  10 meters).

      The crew required  for normal  sampling operations  consists of a pilot and
 two  sampling personnel.  The aircraft can accommodate  two additional sampling
 personnel or observers.  The three sampling  systems are identical  except for
 the  sizes of the winches  and the maximum  sampling-depth capabilities.  Two
 systems  can  sample to a maximum depth of  80 meters  (263 ft) and the third
 system can  sample to  a  maximum  depth of 172 meters  (555 ft).

      The system is capable of  in-situ measurements of:  temperature, conduc-
 tivity,  dissolved oxygen,  pH and  turbidity (Table  4).

      The water-sample cabinet can  hold  576 125-ml  (4-oz) samples  in  storage
 racks.   Additional  samples may  be  stored  in other  locations within the  air
 craft, as necessary.  Water samples  of  any volume can be collected  at a  rate
 of 7  1pm  from any depth up to the  maximum.  The  sampling pump  can  be operated
while the winch  is  retrieving the  sampling assembly, in order  to  collect a
depth-integrated  sample of the  water column.   The  total number of  samples
that can be  collected is  limited by  the storage  space in the  aircraft.   The
water samples collected may be  used  for a  variety of water quality  analyses
 from water chemistry  to phytoplankton analyses.   If the pump  and hose/cable
                                     36

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   TABLE 4.  SPECIFICATIONS OF THE IN-SITU WATER  QUALITY  MONITORING  PACKAGE
Parameter
     Range
 Precision*
Conductivity

Temperature

Depth

DO

pH

Turbidity
0 to 65,000 ymhos

-5 to 45°C

0 to 100 m

0 to 20 mg/1

2 to 12 pH

0 to 100% transmission
±10 ymhos

± 0.02°C

± 0.3 m

±0.2 mg/1

± 0.05 pH

± 2% transmission
* From the manufacturer's specifications.


were replaced with a Teflon pump housing and Teflon  hose in  the  hose/cable,
the system would be satisfactory to sample water for pesticides.

     The system has most comnonly been used to sample in lakes and other non-
flowing waters.  However, it has also been used to collect surface data  and
water samples in rapidly flowing streams,  e.g., some Atchafalaya  study sites.
In this case, the in-situ package was not  lowered through the  water column,
because difficulties in maintaining orientation of the helicopter in flowing
waters enhance the hazard of snagging the  sensor package or  fouling the  pump
system.

     Bottom sediment samples can be collected with a small,  hand-operated,
clamshell-type dredge.  More sophisticated sediment  samplers have been used
from this sampling platform, e.g., cores using a Pflegar Corer,  but there is
not sufficient room in the cabin to do this type of  sampling without removal
of the water quality sampling hardware.  As we used  it, the  Pflegar Corer
also required a cut-out in the floor of the craft and removal  of the athwart-
ship auxiliary fuel tank.
                                     37

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                                    DISCUSSION


      Originally,  three identical,  off-the-shelf,  in-situ water-quality
monitoring/sampling systems v/ere procured.   Throughout  the 4 field years of
NES,  for  an  additional  2 field years  on Atchafalaya  Basin Water Management
Study,  and  during other subsequent studies,  the  systems v/ere constantly being
modified  to  better fulfill  our requirements.

      Helicopters  are  unique and versatile sampling platforms, however, they
do  have certain  limitations.  Space and weight capacity for samples and
equipment,  and electrical  power for sampling  hardware are usually limited
aboard  a  helicopter.   Mechanical  vibration  is usually quite severe.  Light-
weight, compact  sampling and measuring  hardware  helps to reduce the hardware
load  and  maximize available working space for sampling  personnel.  Sample-
storage containers  that are designed  to optimize  otherwise unusable space are
a considerable advantage.   Safety  considerations  of  personnel, sampling
hardware  and the  craft  itself are  best  fulfilled  by  securing all equipment and
sample-storage containers  during  flight.  Small,  light-weight, overboard
sensors and  samplers  increase the  speed of sampling  operations.  During the
first year the system was  in use,  when  frequent  clogging of the original pump
impaired  our sampling efforts,  v/e  considered  changing to a "bottle type" water
sampler.  At that tine  we  concluded that there was neither the space to carry
five or six bottla  samplers in  the aircraft nor tine to make the nultiple
casts at  a  single station  required to sample  from several different depths
with a  single bottle  sampler.  When sampling  from a  helicopte^ to maximum
depths  of about  170 meters, the pump-sampling system is the desirable
technique.   It is believed  that the maximum depth that would be practical  to
sample  with a pump-sampling system in a Huey  is probably about 350 meters.
Sampling  from greater depths with  a pumping system would require a larger
winch than would  be practical in this aircraft.

     Electrical  power is limited on helicopters and  is usually 12 or 24 volts
d.c.  It  is more  efficient  to operate the sampling hardware directly from  the
aircraft  power, whenever possible,  to minimize the amount of power transformed
to other  voltages.  The  exception  to  this is  when a  large amount of pcwer is
required  remote from  the craft, e.g., a submerged electric pump.  The power
dissipated in the wires  becomes significant when the wires are long and small,
or when the currents  are high (greater  than 1A).  In this case the power
should be converted to  a.c.  and transformed to some higher voltage.  The
remote  equipment  should  be  selected for high  voltage, low current require-
ments.  Certain types of equipment  require 117 V a.c. power (e.g., drive
motors  in some strip  chart  recorders).  With  such equipment,  an inverter must
be used to convert  aircraft power  to  117 V a.c. 60 hertz.  Some inverters  have
poor or non-existent  filtering  in  the input circuit which results in the


                                      38

-------
reflection of electrical noise back into the aircraft power bus.  This
power!ine noise and other electrical noises indigenous to aircraft can mask
the data signals in the measuring equipment and possibly lead to generation of
erroneous data.  Dissolved oxygen and pH sensors and circuitry are particu-
larly susceptible to electrical noise interferences and ground loops.  If
multiple pieces of equipment are to be used in the water simultaneously, they
may also exhibit some interaction with one another.

     Vibration levels in aircraft, particularly rotor-winged craft, are high
and potentially damaging to electronic hardware and water quality sensors,
especially the very fragile pH electrodes.  Shock-mounting of all stationary
components and cushioned restraining cradles for on-board storage of all
deployable systems will  reduce vibration damage.

     The sensor/sampling systems on the whole functioned quite well; however,
they do require constant attention in terms of calibration and maintenance.
The dissolved oxygen and pH sensors are those rcost vulnerable to damage and
drift from abuse.

     One problem, quite  unique to helicopter water-sampling platforms was
discovered.  The downwash from the rotor blades of the helicopter physically
disturbs the water's surface layers.  A brief dye experiment conducted at
Lake Mead (Figure 14) indicated the disturbance extended to at least 1 meter
depth directly below the craft.  The upper 20 to 50 centimeters move outward
radially from the landing site and that water is replaced by upwelling water
from below.  While the full  extent of this effect is not known, it is apparent
that all structure in the upper meter of the water column is destroyed.

     The helicopter has  been demonstrated as a cost-effective sampling plat-
form when many sites must be sampled and the travel  time between sites is
excessive in relation to the time required for actual  sampling.
                                     39

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                                                              •, . i  •..••ivii...'ii.'.-,-"  •:.••;••• ';.     .i- , •






                                                              ::::
Figure-14   Dye^ludy  of jleVlcopter^otorwash-^urbulence /ffects on Lake Mead.

-------
                                  APPENDIX A

                         THE DISSOLVED OXYGEN CIRCUIT


     The dissolved oxygen sensor produced by Yellow Springs Instrument Company
functions as a variable-current source when polarized with a 0.7 V d.c.
potential; the current is directly proportional  to both the partial  pressure
of the oxygen and the temperature of the membrane and the sensor assembly.
The sensor current is converted to an analog voltage by the operational
amplifier (op-amp) (AD 523 L) and the feed-back  resistor network (560 K and
250 K trimpot).  The sensor current and the output of the "current to voltage"
transducer are zero in a zero mg/1 oxygen environment.  The op-amp output
voltage, Vo, is calculated by Vo = -I x Rf.

     The analog multiplier (AD 532 S) has differential inputs for both "X"
and "Y" and its output voltage is Vo = (X1-X2)(Y1-Y2)/10.  The output of the
op-amp, a positive voltage, is connected to the  negative "X" input of the
multiplier.  The positive "X" input is grounded.  The temperature analog
output (T), from the temperature board is connected to the positive  "Y" input.
The negative "Y" input is connected to a constant +3.25 v d.c.  This voltage
is developed by the adjustable voltage divider connected to pin 10 (-"Y"
input).  The voUage-te-Tiperature relationship is 0.1 V d.c. = 1.0°C.  The
algebraic representation of the complete circuit is DO (0.1 V = 1.0  mg/1) = -I
x 0.67 (T - 3.25J/10.  This is a linear approximation of the second  degree
polynomial DO = 0.7945 I/(0.0104 T2 + 0.2045 T + 3.7879) that approximates the
sensor performance between 0° and 25°C.  The linear approximation was adjusted
to minimize differences (±2%) from the polynomial  in the temperature range of
6°C to 19°C.

     The MC 1568 C is a voltage regulator circuit providing bipolar  (+ & -)
15 V d.c. required for the multiplier.  Other modules in the sensor  package
operate on the original  bipolar (+ & -) regulated 8 V d.c.  The voltage
divider from the negative supply, the 33/K and 1.6/K resistors to ground,
provides the polarizing voltage for the sensor.

     Before calibrating the oxygen circuit, the  temperature calibration should
be assured.  To calibrate the circuit and sensor cc.Tibination, the following
items capabilities are required:

     A 3^-digit digital  voltmeter, a source of dry nitrogen or helium
     gas, water in the 10°C to 15°C range that is  nearly saturated with
     oxygen, and the capacity for a Winkler titration for oxygen
     determination.   The sensor is first bathed  in a stream of the gas
     to exclude oxygen from the membrane.  While this zero oxygen
     environment is  maintained at the membrane,  the 10 K trimpot

                                     41

-------
connected  to  pins  1, 4  and 5 of  the op-amp (AD 523 L) is adjusted
to yield 0.000 V d.c. at tp-1.   Next the 20 K trvnpot connected
to pins 2, 5  and 9 of the multiplier (AD 523 S) is adjusted to
yield 0.000 V d.c. at tp-2.  At  this time the sensor is removed
from the zero oxygen environment.  The 10 K trimpot connected to
pin 10 of  the multiplier is adjusted to yield +3.250 V d.c. at
tp-3.  A sample of the  water to  be used for the instrument calibra-
tion is then  evaluated  for oxygen concentration using a Winkler
titration; the temperature and oxygen sensors are placed in the
remaining  water.  The water is gently stirred while the temperature
and oxygen sensors are  allowed 2 minutes to reach equilibrium with
the water.  Once equilibrium is  achieved, the 250-K trimpot in the
"feedback" circuit of the op-amp (pins 2 and 6 of AD 523 L) is
adjusted to yield an analog of the dissolved oxygen concentration,
found from the Uinkler  titration, at tp-2 (+0.1 V d.c. per 1  mg/1  of
oxygen).   This process  may be repeated to verify stability.
                                42

-------
          15Vil c
 TP
1
From Output of Temp Analog
                     5 G
                                         33
                                                           AD532 S
                                                  1 Y1 •

                                                  10 YZ -
—oTP
 100 K
                                                       •IGViJ c
Power Input


1fU/,l , „


Giul — » .

I
1
MC
15G8
C
6
t/vwv
& G


i


U
f



£

T°
-i-E
' o
o -1
0 ~
33 K 5
1 G K<
f
r:^. >.n>

~L
_2 y,,,[



                                                                                      TP
                                                  IbVd c
                                                           *- OUTPUT
                                                                         •15Vclc -ISVdc
                                                  15Vt| c
                                                  0 7Vti c   10 Oxygen Sensor Shield
                        Figure A-l.   The  dissolved  oxygen  circuit.

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                                   APPENDIX  B

                   THE DIFFERENTIAL-INPUT pH-MEASURING  CIRCUIT


     The  glass  pH-measuring electrode  and the  Lazaran  reference electrode
 function  as  a  variable voltage source  with  an  extremely  high internal
 impedance (10+6  to 10+s  ohms).  The  output  potential of  the pair is zero at pH
 7.68; the voltage  analog from the electrode pair  is  59.2 millivolts per pH
 unit.  The temperature factor is 0.77  millivolts  per degree to be sub-
 tracted above  25°C and added below 25°C.

     The  high  impedance  of the sensor, pair  requires  the  use of an amplifier
 with a high-input-impedance.  To minimize system  susceptibility to electrical
 noise and "ground  loops,"  a high-input-impedance  differential-buffer-amplifier
 (HA2005)  was placed  in the sensor mounting  block.  The input leads from the
 electrodes to  the  amplifier were about 1 cm long.  The buffer amplifier has
 an input  impedance of IQiz  ohms  and  unity voltage gain.  The 100 K trimpot
 connected to terminals 1  and 5 is the  balance  control and is adjusted for zero
 output in a  pH  7.00  buffer.  The differential  output of the buffer drives the
 differential inpuc of the  instrumentation amplifier  (AD 520 K).  The gain of
 the device is controlled  by the  ratio  of the 100 K resistor (pins 9 and 11)
 and the 47 K resistor and  10 K trimpot (pins 5 and 7).  This gain function
 raises the analog  from 59.2 millivolts per  pH  unit to 100 millivolts per pH
 unit.  The offset  terminal  (pin  12)  is ulilized to shift the sensor zero
 output voltage corresponding to  a pH 7, to  -0.7 volts or the 100 millivolt per
 pH unit analog.  The  negative output is  inverted by the following stage.

     The  output  inverter  stage (AD 741  K op-amp) accomplishes the temperature
 correction of the  sensor.   The temperature  correction is a linear additive
 correction.  The 0.77  mv/l°C at  the  sensor  is  the equivilant of 0.013 pH units
 per degree.  The zero  point on the temperature correction curve is 25°':, and
0.013 pH  units  is  added  per degree Celsius  below 25°C.  The 1 K and two 76.8 K
 resistors at the inverting  input  of the op-amp comprise a three-input adder.

     The  1,000-ohm "feedback"  resistor from the output to the inverting input
provides  unity gain when the temperature is 25°C.  The 100 K trimpot is set to
-2.5 V d.c., corresponding  to  -25°C, which  is added through 76.8 K resistors
to the positive temperature analog.  As the temperature analog  falls below
25°C (+2.5 V d.c.), the analog temperature  difference from 25°C is  added to
the pH analog at the  rate  of 1,000/76.8 K per degree Celsius (0.013 pH/l°C).

     This circuit  is  powered by  the original regulated 8-volt supply.
                                     44

-------
     Before calibrating pH, the temperature calibration should  be  assured.
To calibrate the circuit, the following items  and  capabilities  are required:
two Celsius thermometers with 0.2 degree or finer  graduations which include
25°C in their range, a 3 1/2-digit digital  voltmeter,  pH 7  and  10  buffer
solutions, a 100-ml  beaker, a 250-ml  beaker,  a 500-ml  beaker, and  a source  of
25°C water for temperature control.

     The pH 7 buffer is put in the 250-ml  beaker which, in  turn is put  in a
water bath in the 500-ml beaker at 25°C.  When the buffer is 25°C, the  pH
electrode assembly is immersed in the buffer  and the 100 K  balance trimpot  is
adjusted to yield 0.000 V d.c. between TP-1 and TP-2.   All  following voltage
measurements are made referenced to TP-7 (ground).  The 10  K offset trimpot  is
adjusted to yield +0.700 V d.c. at TP-3 and the 500-ohm zero trimpot is
adjusted to yield 0.700 V d.c. at TP-4.  The  100 K temp-cal-comp trimpot is
adjusted to yield -2.5 V d.c. at TP-5.  With  25°C  water on  the  temperature
sensor, the 10 K zero trimpot is adjusted  to yield +0.700 V d.c. at TP-6.  The
pH electrodes are thoroughly rinsed and immersed in pH 10.18 buffer at  25°C,
and the 10 K gain trimpot is adjusted to yield +1.018  V d.c. at TP-6.

     The process should be repeated to verify  linearity.  If the expected pH
to be measured is below pH 7 the pH 10 buffer  should be replaced with pH 4
buffer and the gain  trimpot adjusted  to yield  +0.400 V d.c. at  TP-6.
                                     45

-------
-is.
en
        Input
        From
        Sensor
        Prenmp
                                                                                                      100
                                                                                                             •BVtl c
                                                                                                             8V.I c
                              SENSOR PREAMP
                                  HA-2005
                            Figure  B-l.   The differential-input and  pH-measuring circuit.

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                                  APPENDIX C

                 WATER COLUMN FOR CALIBRATION OF ECHO SOUNDER


     The calibration of the echo sounder posed a special  problem.   A water
column was necessary for depth calibration and for electronic  alignment  of
the driver and receiver circuitry.  An artificial  water column was  fabricated
from a length of 4" A3S sewer pipe,  a pipe cap and a  plastic trash  can.   A
piece of 1/2-inch thick plastic was  mounted at a 45°  angle  in  the trash  can
as a reflector.  The 4-inch ABS pipe was cut to a  length  such  that  the path
length from the transducer to the 45° reflector and down  the pipe to the pipe
cap was exactly 10 feet.  The trash  can/pipe assembly is  placed under the
transducer with the pipe lying on the ground.  The pipe and trash can are
filled with water to cover the transducer.  Electronic alignment of the
circuitry is accomplished by adjusting for the greatest number of reflections,
or with an oscilloscope.  Calibration is accomplished by  adjusting  the speed
of the revolving disk.
                                     47

-------
              10 Ft.
00
                           Trash Can
                          Echo Sounder Transducer
                                                                       4" ABS Sewer Pipe
                                                  7
                                                                                            Pipe Cap
              45° Reflector
            Figure C-l.  Ten-foot water  column for calibration of the echo sounder on  the helicopter
                                             but out of the water.

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                                  APPENDIX D

                           CALIBRATION BUFFER BOARD


      Calibration of the original sensor package required disassembly of the
pressure case to access the calibration controls.  A five-channel analog
buffer was designed and installed on one system to evaluate the performance of
the circuit.  Evaluation was never completed.  The circuit of a single buffer
amplifier is shown.  The concept is to be able to offset the zero point ±0.5
volts and to vary the gain from 0.6 to 1.5.  The five-channel  buffer board
was mounted on the read-out console with the screwdriver-settable zero and
gain controls accessible from the front of the console.  Power for the buffers
was taken from the read-out console.  This circuit should reduce the need to
open the pressure case for calibration to a rate of only one calibration in
four.
                                    49

-------
un
O
            input from insitu package
       10k
            100k
•WVA/V	-WWWWAA
         •^  10k
                                                                                   15Vd.c.
                                               Output to

                                             Console Input
                                                                                   -15V d.c.
                    Figure D-l.   Schematic of  one channel  of calibration buffer amplifier.

-------
                               LITERATURE CITED


U.S. Environnental  Protection Agency.   1974.   national  Eutrop'nication  Survey
     Methods for Lakes Sampled in 1972.   National  Eutrophication  Survey
     Working Paper No. 1.   Environmental  Monitoring  and Support Laboratory,
     Las Vegas,  Nevada,  and Environmental  Research Laboratory,  Corvallis,
     Oregon.  40 pp.

U.S. Environmental  Protection Agency.   1975.   National  Eutrophication  Survey
     Methods 1973-1976.   National  Eutrophication Survey Hor'r-.ing Paper  No.
     1975.  Environmental  Monitoring and  Support Laboratory, Las  Vegas,
     Nevada, and Environmental  Research Laboratory,  Corvallis, Oregon.  91 pp.
                                    51

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                                    TECHNICAL REPORT DATA
                             (P'easereadI>'.s:r.'.c::o".soi: •! s reverse oetcrs -omp!e.'"-gi
    = ORT"O
   EPA-600/4-31-005
                                                            13 RECIPIENT'S ACCESSION 'J
   ITLE -WO S'_'3T:TL =

    DEVELOPMENT OF A HELICOPTER V.'ATEP QUALITY
    .'IONITORiNG/SAMPLIMS SYSTEM
5 REPORT DATE
  February  1931
           ORiiANIZATiG.V CO^r
                                                            8 PEHrORMI.\S ORGAi\!ZATIOiii R=?C
    H. Michael  Lov/ry
9 PERFORMING GRGANiZATION NAME AND ADOR2SS
 Environmental  Monitoring  Systems  Laboratory
 Office of Research and Development
 U.S. Environmental Protection Agency
 Las Vegas,  NV   89114
10 PROGRAM ELEMENT NO
11 CONTRACT, OR-,NT .\O
                                                                                           s
 12 SPONSORING AGENCY NAME A.\O ADOnESS
 U.S. Environmental  Protection Agency--Las  Vegas, NV
 Office of  Research  and Development
 Environmental  Monitoring Systems  Laboratory
 Las Vegas,  NV   89114
I 12 TV?E CF
             T AND PERIOD COVEflcO '•
14 S
 15 SUP°LE.V1ENTAHY \'OTHS
            AGfc'ICv COOE
     EPA/ 600/0 7
16 ABSTRACT
         This  report describes the helicopter water quality sampling system  developed
    for use by  the  Nacional Eotrophication  Survey and subsequently used in support'
    of other water  quality projects.   It  also describes the salient oroblens
    associated  vrlththe use of a helicopter  as a sampling plat^crm and t^e iiudifications  '
    medejn the system to fulfill cur  needs.   This system is useful to other  groups      '
    involved in sampling large numbers of water quality sites distributed over  large
    or inaccessible areas.

17 KEY V/ORD3 AND DOCUMENT ANALYSIS
a DESCRIPTORS
Ai rcraf t
Helicopter
Water pollution
3 6lSTPI3UTiCiV S rATEMENT
RELEASE TO PUBLIC
b IDENIIrlEHS/OPE.Vi SNCED TE.R.V.S
National Eutrophi cation
Survey
Airborne platforms
19 SECURITY Cu/SS , H.is Rfac.-:)
UNCLASSIFIED
20 SECUS!TV CLASS 'T- 'j pceel
UNCLASSIFIED "
o COSATI I icId'C'CJ^
48C
68D
j
j
?.' NO Or PAGES
^ 61

EPA Form 222C-! (Re- :-77!
                              ED! TIOM i s O33O1.ET e

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