LAKE  EUTROPHICATION:

RESULTS FROM THE NATIONAL EUTROPHICATION SURVEY
                   .  By

      Jack H. Gakstatter,  Marvin  0. All urn
             and James M.  Omernlk
  Presented at the 26th Annual AIBS Meeting;
  Oregon State University, CorvalUs,  Oregon
              August 17-22, 1975
  Corvallis Environmental Research Laboratory
     U.S. Environmental Protection Agency
      Office of Research and Development
            CorvalUs, Oregon 97330

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INTRODUCTION
    In early 1972,  the U.S.  Environmental  Protection Agency  (EPA) initiated
the National Eutrophication  Survey (NES)  program to:   (1)  identify those
                                                                      i
lakes and reservoirs in the  contiguous United States that  receive nutrients
from the discharges of municipal  sewage treatment facilities, and  (2)  deter-
mine the significance of these point-source nutrient inputs  to  the nutrient
levels and the primary productivity of each system.  After the  program began,
additional federal legislation was passed (Public Law  92-500),  and NES
objectives were broadened to include an assessment of  the  relationships of
non-point sources; e.g., land use, to lake nutrient levels and  also  to assist
in establishing water-quality criteria for nutrients.
                        Selection Criteria
     Freshwater lakes  and impoundments in the Survey were selected through
consultation with  EPA Regional Offices and state pollution control agen-
cies,  as well as related state agencies managing fisheries, water resources.
or public health.   EPA established selection criteria to limit the type
and number  of candidate water bodies, consistent with existing Agency
water goals and  strategies.  For 27  states of the eastern United States
where lakes were selected prior  to passage of P.L. 92-500,  strongest
emphasis was placed on lakes faced with  actual  or potential accelerated
eutrophication  problems;  i.e., an artificially  increased  rate of algal
and/or aquatic  plant production. As a  result,  the selected lakes:
         1.   were impacted by one or  more municipal sewage treatment
     plants, either directly or by discharge  to  an  Inlet tributary
     within  approximately 25 miles of the lake;

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        2.   were 100 acres or larger In  size;  and
        3.   had mean hydraulic retention times of at  least 30 days.  .
                                                                  »t
However, these criteria were waived for  a number of lakes of particular
interest to the states.
    In the western states, these criteria were modified  to reflect
revised water-research mandates, as well as to address more prevalent
non-point source problems in agricultural or undeveloped areas.  Thus
each state was requested to submit a list of candidate lakes for the
Survey that:
        1.  were representative of the full range of  water
    quality (from oligotrophic* to eutrophic*);
        2.  were in the recreational, water supply, and/or
    fish and wildlife propagation use-categories; and
        3.  were representative of the full scope of  nutrient
    pollution problems or sources (from municipal waste  and/or
    nutrient-rich industrial discharges, as well as from non-
    point sources).
    The size and retention time constraints applied  in the eastern  states
were retained as was the waiver provision.
    In all cases, listings of potential  candidate  lakes  or reservoirs,
prepared with the cooperation of the EPA Regional Offices, were made
available to the states to initiate the selection  process.
    In total, the Survey includes 812 lakes and reservoirs across the
contiguous 48 United States.  Figure 1 shows the distribution of the

* Oligotrophic—low nutrient concentrations and primary  productivity.
  Eutrophic—high nutrient concentrations and primary productivity.

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1975-152
1973-250
  GRAND TOTAL-  812
   Figure 1.  Number of lakes and  reservoirs sampled 1n each state and yea'r of
             sampling by the National Eutrophication Survey.

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lakes and reservoirs by state and the year during which each water body
was sampled.
GENERAL SURVEY METHODS
    Several kinds of Information are required as a  basis  for management
decisions regarding the need for point or non-point source control of
phosphorus and perhaps other nutrients as well.  The Survey purpose  1s
to collect the type of data which will provide a basis for such decisions
or at least to provide a data base which can be supplemented with more
detail, if required.  First, an annual nutrient budget is estimated  for
each water body, differentiating between inputs originating from point
and non-point sources; second, the existing trophic condition  of the
water body  is evaluated by sampling; and third, an algal  assay 1s per-
formed  to determine whether  phosphorus, nitrogen,  or some other element
is limiting primary productivity of  the water body.  The methods used to
gather  this information are  described below.
     The operations  aspects of the Survey are shared by branches of two
.EPA  laboratories  (46  people) and a small headquarters staff (3 people).
The  Environmental Monitoring and Support Laboratory at Las Vegas, Nevada
 (Las Vegas-EMSL)  is responsible  for  sampling each  lake, doing the associ-
 ated analyses,  evaluating a  portion  of  the data, and  reporting results.
 The  Corvallis Environmental  Research Laboratory (CERL) at CorvalUs,
 Oregon is.responsible for coordinating  the sampling of streams and sewage
 treatment plants,  analyzing  the  samples,  and  performing  the algal assay
 on lake samples.   CERL also  has  major responsibility  for evaluating the

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lake, stream, and point-source data  and  Incorporating these data Into a
report on each lake.  The headquarters staff  (Washington, D.C.) makes, the
Initial contact with each state water pollution  control agency to expiain
the function of the Survey and to cooperatively  determine which lakes and
reservoirs will be Included.  They also  contact  each State National Guard
to explain the function of the Survey and to  request their assistance In
meeting Survey objectives by collecting  monthly  samples from  selected
tributaries to surveyed lakes.  In addition,  the headquarters staff
provides general coordination and guidance to the operational aspects of
the program.
    Because the Survey has to cover a large geographical  area 1n a rela-
tively short period of time, pontoon-equipped UH-1H Bell  helicopters with
automated and manually-operated instruments are used to measure  the water
quality of each lake.  Two helicopters - carrying a Hmnologlst  and a
technician - are operated simultaneously, and a third helicopter is used
for  ferrying parts, equipment, and people.  The sampling  teams from the
Las  Vegas-EMSL are  sopported by a mobile analytical laboratory, chemistry
technicians, electronic  specialists, and other staff involved with heli-
copter maintenance or program coordination.  The total staff 1n  the field
usually ranges from 12 to 14 people.
     Operating  procedures involve establishing a work center at an airport
and  then  sampling all lakes within a 100-mile radius.  When all  of the
water  bodies within the  area are sampled, the support staff moves to  a
new  central  location, and sampling begins on a different set of lakes.  In

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this manner, 150 to 250 lakes have been sampled three times  each year,  and

the sampling will be completed on all  of the 812 lakes In a  four-year period.

    Table 1 depicts the routine water-quality parameters which were sefexted

to characterize each lake and assess Its trophic condition.   Parameter

selection was based on the relevance of each parameter as a  measure of

potential and existing primary production.  Both the number  and the type

of parameters measured were also limited to a certain extent by the opera-

tional aspects of the Survey.

          Table  1.  Water-quality characteristics measured
                         Physical-Chemical
            Alkalinity
            Conductivity*
            pH*
            Dissolved oxygen
            Phosphorus:
               Ortho
               Total
Nitrogen:
  Ammonia
  Kjeldahl
  Nitrate
Seech1 depth
Temperature*
                             Biological
             Algal assay
                            Chlorophyll  a.
Algal count and
 Identification
             * Determined on-s1te with electronic probes.

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    Concurrent with the lake  sampling,  the  significant tributaries and
outlet(s) of each lake are sampled monthly, totaling about 4,200 sampling
sites nationwide.  Volunteer National Guardsmen of each state, trained
on-site by EPA or state agency staff, collect and preserve the samples'^.  ,.
at sites pre-selected by EPA personnel.  The samples are  shipped to CERL
for analysis of the various forms of nitrogen and phosphorus  (see Table 1).
    Through an interagency agreement,  the U.S. Geological Survey estimates
flows for each sampled stream.  These data are used in conjunction with
concentration values to determine nutrient loadings.
    A voluntary  sampling program was established through the respective
state water pollution  control agencies to  have plant operators collect
effluent samples from  those municipal  sewage treatment plants which
impact  Survey lakes—about 1,000  treatment plants.  The effluent samples
are collected monthly, preserved,  and  shipped  to the Corvallis laboratory
for nitrogen  and phosphorus  analyses.
     Specific  procedures used In collecting,  preserving,  shipping, and
analyzing the various  kinds  of samples collected  by the  Survey are
 described In National  Eutrophication Survey Working Papers No. 1 (1974)
 and 175 (1975).
     Presently, the field portion of the  Survey is almost completed with
 the last samples scheduled for collection in November, 1975.  Data
 analysis is scheduled for completion in  December, 1976.

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                                 8
RESULTS AND DISCUSSION
                        Limiting Nutrients
    For each of the surveyed lakes,  an  algal  assay  1s performed on a
sample of lake water and, to supplement the assay findings, Inorganic
nitrogen to dissolved orthophosphorus ratios  are  determined from the
lake sampling results.  For the 623  surveyed  lakes  1n states east of
the Rocky Mountains, the assay demonstrated that  with respect to algal
growth requirements, 67% were phosphorus-limited, 30% were nitrogen-
limited and 3% were either limited by an element  other  than phosphorus
or nitrogen or the results were not  conclusive (Table 2).

       Table 2.  Summary of algal assay results for surveyed
     water bodies in the 37 states east of the Rocky Mountains.
Limiting Nutrient
Phosphorus ,
Nitrogen
Other
Total
Number of Lakes
417
186
20
623
% of all Lakes
67
30
3
100%
    A higher.percentage of phosphorus limited lakes would probably have
been found had the Survey not been mostly concerned with lakes which were
impacted by municipal wastes.  The algal assay results should, therefore,
be evaluated with some caution because they reflect existing conditions
which often include man's impact on the nutrient regime.

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    Municipal waste treatment plant effluents,  for example,  have an average
total nitrogen to total phosphorus ratio of about 2.5 to  1 whereas natural
waters usually have a ratio 1n excess of 15 to  1.  The relative abundance
of phosphorus provided 1n municipal effluents could change a lake from
phosphorus-limited to nitrogen-limited.  Such a lake could theoretically
be changed back to phosphorus-limited by reducing phosphorus Inputs.
    Figure 2 Is an Indication of the significance of municipal wastes to
the total annual phosphorus load to some of the eastern lakes and reser-
voirs.  Of the 234 water bodies Included 1n the frequency histogram,  135
receive more than 20% of their annual total phosphorus load  from municipal
sources.  If 80% of the phosphorus were removed from these discharges by
treatment, only 9 of the lakes would still receive more than 20% of their
total phosphorus load from municipal wastes as shown 1n Figure 3.
    The reduction or removal of phosphorus originating from municipal
sources does not guarantee that the trophic status of the receiving lake
will  be significantly  improved.  That determination can only be made on
a  case-by-case basis In which many factors, such as background phosphorus
levels, the  limiting nutrient, lake morphometry, etc., are considered.
 It is apparent, however, that in many  cases, eutrophlc conditions are
either the direct  result of  phosphorus  from municipal wastes or at least
are worsened by phosphorus Inputs  from these sources which could be
 readily controlled.

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   200

    175
2  ISO
J   125
u.
°   IOO
or
m   75
2
^
z   50

    25
         0-10   11-20  21-30  31-40   41-50  51-60  61-70  71-80  81-90  91-100

    PERCENT OF TOTAL  PHOSPHORUS  LOAD  FROM   MUNICIPAL  POINT  SOURCES
           Figure 2.  A frequency histogram representing the percent of total annual
                    phosphorus load attributable to municipal wastes for,a-'number
                    of eastern U.S. lakes and reservoirs.

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   200


    175
s  150
u.
0
    125
    100
    50


    25
         0-10   11-20  21-30  31-40  41-50  5I-6O  61-70  71-80  81-90  91-100

    PERCENT  OF TOTAL  PHOSPHORUS  LOAD  FROM  MUNICIPAL  POINT SOURCES
                            FOLLOWING  80%  REMOVAL
          Figure 3.  A frequency histogram representing the percent of total annual
                   phosphorus load attributable to municipal  wastes after Q0%
                   effluent phosphorus  reduction for a number of eastern.if.S lakes
                   *nd reservoirs.

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                                 12
                 Trophic Condition  of Survey Lakes
    About 80% of the lakes and  reservoirs  Included 1n the first two
years of the Survey 1n the eastern  United  States were eutrophlc.  Th'ts
was not unexpected since a large number of these water bodies were
impacted by municipal wastes.
    The classical terms, oUgotrophlc,  mesotrophlc, and eutrophlc were
used to describe the trophic condition  of  each water body.  Based partly
on observations during the first year of the Survey and partly on litera-
ture values, some general guidelines were  developed for each of four key
parameters to assist us in assigning a  trophic classification to each
lake.  These values are listed  in Table 3.
          Table 3.  Key parameter values associated with
                 three lake trophic conditions.
Parameter
Total Phosphorus (yg/1)
Chlorophyll a (yg/l)
Seech i depth (meters)
Hypolimnetic Dissolved
Oxygen (% saturation)
OUgotrophic
<10
< 4
> 3.7

>80
Me so trophic
10-20
4-10
2.0-3.7

10-80
Eutrophlc
>20-25
>10
<2.0

<10
     If each of the four parameters from a given lake were within  the
range of a specific trophic condition (e.g., oUgotrophic) then it was
fairly certain that the Indicated trophic condition appropriately

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                                13
described the lake.   Unfortunately,  In many cases, all the parameter
values did not neatly fall  wUMn one trophic  classification; therefore,
a relative Index or ranking system was  also used.  This  Index Included.
the four parameters shown 1n Table 3 (except  that  minimum dissolved
oxygen concentrations were used) plus Inorganic nitrogen and dissolved
orthophosphorus concentrations.  The Index was based on percentile
rankings for each of the six parameters which were then added together
to  produce a single  index number.  Using this system, a large number of
lakes could  be ranked  1n general order from most oligotrophlc to most
eutrophic.   There were enough  well-studied lakes Included In the Survey
 to allow us  to determine approximately where  the transition from oligo-
 trophic to mesotrophic and from mesotrophic to  eutrophic occurred in
 the ordered list of lakes.  This system  was not without exception but
 did prove useful.   The Index is discussed 1n  detail  in  National Eutrophi-
 cation Survey Working Paper No. 24 (1974).
        Phosphorus Loading - Trophic Condition Relationships
      Another of the Survey objectives was to estimate annual phosphorus
 and  nitrogen loadings  for each of the study lakes and to examine rela-
 tionships between these nutrient inputs and the resulting trophic condi-
  tions.  Such relationships are needed by lake managers to predict trophic
  responses which would result  from either Increasing or decreasing phos-
  phorus  loads.  They would also give regulatory agencies a  firmer basis

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                                 14
for allocating total  phosphorus loads  from point or non-point sources
so that the desired trophic condition  of a lake or reservoir could be
maintained or achieved.
    The Survey has not developed any original  nutrient  loading-lake
response relationships.   However, the  data have been  applied to models
recently developed by other Investigators.
    Prior to 1968 there were no models of general applicability which
related total phosphorus load to trophic condition  In the receiving  lake.
Now, however, there are at least three which seem very  promising.  These
models are presented and compared using data collected  by the  Survey
from twenty-three lakes and reservoirs.  These twenty-three water bodies
represent a cross-section of trophic conditions,  mean depths,  and mean
hydraulic retention times.  All are located in northeastern and north-
central states except for two  reservoirs 1n Georgia and two in South
Carolina.   In this group of lakes, six are oligotrophlc, nine are
mesotrophic, and eight are eutrophic.
    The three relationships (or models) which will  be compared were
developed  by Vollenweider and  Dillon  (1974), Dillon  (1975), and Larsen
and Herder (1975),  respectively.
    Vollenweider  (1968), using existing data from a  number of European
and North  American lakes, was  the  first to  relate total phosphorus
loading to lake trophic condition.  He plotted annual  total phosphorus

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                                 15
loadings (g/m2/yr) against lake mean  depths  and  empirically determined
the transition between oligotrophic,  mesotrophic,  and  eutrophic loadings.
                                                                   *'t
    Although this approach worked reasonably well  for  lakes with detention
times of several months or longer, it did not account  for the fact  that
two lakes with identical mean depths  could have  quite  different hydraulic
retention times and therefore different trophic  responses to  the same
loading rate.  Subsequently, Vollenweider modified his initial relation-
ship and based his revised model on considerations of  a mass  balance
equation for phosphorus.  The application of Vollenweider's  revised
model to the Survey lakes is Illustrated in Figure 4.
    The observed  loadings and trophic conditions of the 23 Survey
lakes did not fit the Vollenweider relationship very well.  Phosphorus
loadings for five of the eutrophic lakes plotted clearly within the
eutrophic zone  of the Vollenweider relationship while loadings of two
eutrophic lakes  plotted within  the mesotrophic zone and one within the
oligotrophic  zone.  Loadings for  five of the mesotrophic lakes fell
within  the  oligotrophic zone while the remainder were within the meso-
trophic portion of  the Vollenweider  relationship.
     Vollenweider's  work was extremely important not only because he
was the first to investigate the  loading-response relationship but also
because his original  ideas  interested others  1n this  type of approach.
     Stimulated  by Vollenweider's  earlier work, Dillon  (1975)  used the
mass balance modeling approach to derive the  relationship illustrated

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       MEAN  DEPTH (m)  / MEAN HYDRAULIC RETENTION TIME (yrs.)
Figure  4.  The Vollenweider relationship  applied to a number of eastern U.S
          lakes and reservoirs sampled b.y  the Survey.

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                                17
in Figure 5.   Dillon's approach  relates  lake mean depth to a factor
which Includes total annual  phosphorus  loading, the phosphorus retention
coefficient, and hydraulic flushing time.   The 23 Survey lakes fit tne^.
Dillon relationship quite well as illustrated  In Figure 5.  Two
oligotrophic lakes plotted in the mesotrophic  zone  and two mesotrophic
lakes plotted in the oligotrophic zone; however, observed conditions
for  the other lakes were as predicted by the Dillon relationship.
     Larsen and Herder (1975), working independently of Dillon,  also
solved  a  mass balance equation  for phosphorus to develop a relationship
between the  average incoming  phosphorus concentration and the phosphorus
 retention coefficient.  The average  incoming  phosphorus concentration
 is defined as the total annual  phosphorus  load divided by the total
 hydraulic inflow which is also equivalent  to:
 where, L = annual total phosphorus areal load (g/m2/yr)
        C * hydraulic flushing time (exchange/year)
        z = mean depth (meters)
     The Larsen and Merder relationship therefore Incorporates the same
 variables as the Dillon relationship although the graphical solution of
 the mass balance model for phosphorus is different.  Figure 6 depicts
 the 23 Survey lakes plotted against the Larsen-Merder relationship.
 The fit  is  very  good and  the  relative location of each point on the
 graph is very similar  to  Figure  5, the  Dillon relationship.

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         EUTROPHIC
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                                    O  Oligotrophic  Lakes
                                    A  Mesotrophic  Lakes

                                    D  Eutrophic Lakes
                                                '    i  i  i  i  i i
0.0
                                 10.0

                     MEAN  DEPTH (METERS)
100.0
                                                                           00
Figure 5.   The  Dillon relationship applied to a number of eastern U.S.
            lakes and reservoirs  sampled by the Survey.

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                                        A  Mesotrophic Lakes
                                        D  Eutrophic  Lakes
               O.I    0.2    0.3   0.4   0.5    0.6   0.7   0.8   0.9
                PHOSPHORUS RETENTION  COEFFICIENT  (
                                                                        1.0
        Figure  6.   The Larsen-Mercier relationship applied to a numbef" of eastern
                   U.S. lakes and reservoirs sampled  by the Survey.

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                                  20
     Since both the latter two models  predict In-lake concentrations of
 total  phosphorus,  the vertical distance  from an observed point, reprer
 senting a lake, to one of the transitional  lines  1s at least a semi- *'-
 quantitative measure of the degree  of ollgotrophlc or eutrophy.
     The vertical distance from a given point to a transitional line 1n
 the Vollenwelder relationship has less meaning 1n terms of the degree
 of ollgotrophy or  eutrophy because  the model  does not directly relate
 total  phosphorus loading  to 1n-lake phosphorus concentrations.
     In summary,  the models developed  by Dillon and Larsen-Mercler,
 which  relate total  phosphorus loads to lake  phosphorus concentrations,
 should prove to be  useful  lake management tools.  The Vollenwelder model,
 at this  time,  Is probably less precise because 1t considers only total
 phosphorus loading  without regard to  In-lake processes which reduce the
 effective phosphorus concentration; however, the model  can be used to
 determine approximate acceptable total phosphorus loads.

         The Relationships of Land Use to Nutrient Levels
    Another of the Survey objectives Is to examine,  on  a National  scale,
 the relationships of land use and other drainage area characteristics to
 stream nutrient levels and subsequently lake trophic status.
    Of the 4,200 sub-drainage areas  sampled by the Survey  across the
United States, about 1,000 were selected for a detailed study of land
use and other drainage area characteristics (see Figure 7).  Criteria

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                                                                             DI1T1IBUTION Of
                                                                             N. E. S UNO USE
                                                                           STUDY MAINAGt AM AS
Figure 7.   The distribution  of stream drainage areas selected  by the
            National Eutrophication Survey  for land use studies.

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                                 22
for selecting the 1,000 stream sampling  sites  and associated drainage
areas were:
    1.  Absence of Identifiable point sources.
    2.  Availability of usable aerial photography  (scale  1:40,000 to
1:80,000) or existing land-use data.
    3.  Availability of accurate topographic maps  for drainage  area
delineation.
    4.  Sufficient land relief for clear delineation of drainage area
limits.
    5.  The need to encompass a variety of geographic and climatic
areas.
    Note that few, if any, of the selected drainage areas were  1n  Florida,
the Atlantic and Gulf coastal plains, or northern Minnesota.  These  areas
were  excluded from consideration because of the difficulty of accurately
defining drainage area boundaries due to low topographic  relief,  and,  in
many  cases, because of the strong influence of ground water.
    At  the present time only the data from the eastern United States
 (east of the Mississippi River) have been compiled, but the analysis
 of these data  is not complete.  Therefore, only general results are
 presented.
     Figure 8  summarizes the data collected from 473 eastern U.S.
 drainage areas for  total phosphorus  and total nitrogen concentrations
 originating from different land use  categories.  The  categories are
 defined as follows:

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NU	
or SUM
 53   FOREST ^

 170   MOSTLY FOREST

 52   MIXED

 11   MOSTLY URIAH

 M   MOSTLY ACR 1C.

 91   AGRICULTURE
                              MEAN   TOTAL  PHOSPHORUS  CONCENTRATIONS
                                                   vs
                                              LAND  USE
                                         DATA ON, 473 SUBCMAINAGE AREAS IN
                                            EASTERN UNITED STATES
                        | 0.014
                                    j 0.031

                                       ] 0.040
                                                         0.066

                                                         0.066
                                            0.03
                                                 MILLIGRAMS PER LITER
                                                                         0.10
                                                                                          0.13S
                                                                                                      O.IS
NUMMD
OF SUBS
 53  FOREST ^

 170  MOSTLY FMEST

 52  MIXED

 11  MOSTLY URMN

 96  MOSTLY MRIC.

 91  AGRICULTURE
                              MEAN TOTAL  NITROGEN  CONCENTRATIONS
                                                   vs
                                              LAND  USE
                                         0*TA ON 473 SUBDRAINAGE *«E»S IN
                                            EASTERN UNITED STATES
                                     O.MO
                                              1.2*1
                                                         l.tlJ
                                      1.0
                                                           1.0
                                                 MIILIGRAMS  PER LITER
                                                                                                                               ro
                                                                                                            4.170
                                                                                3.0
                                                                                                      4.0
                  Figure 8.   The relationship between  total  phosphorus  and  total  nitrogen
                               concentrations  in  streans  and  land  use  in  the  eastern U.S.

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                                24
    1.   Forest;  other  types negligible
        a.  >75% forested  (including forested wetland)
        b.  <7%  agriculture
        c.  <2%  urban
    2.   Mostly forest; other  types present
        a.  >50% forest
        b.  not  included  in the  forest category
    3.   Mostly agriculture; other types present
        a.  >50% agriculture
        b.  not  included  in the  agriculture category
    4.   Agriculture; other types negligible
        a.  >75% agriculture
        b.  <7%  urban
    5.   Urban
        >39%  urban
    6.   Mixed; not included  in any of other categories
    Streams draining  predominately agricultural  areas have  total phos-
phorus  concentrations  averaging  about 10  times higher than  those
draining forested areas (Figure  8).  The  difference between total
nitrogen concentrations was  not  as marked.  Streams in  agricultural
areas averaged nearly 5 times higher total nitrogen concentrations
than those draining forested areas.   It  is  Interesting  to note  that,
based on the  mean concentration  values,  phosphorus would be expected
to be limiting in surface waters draining either forested or agricul-
tural areas.   The total nitrogen to  total phosphorus ratio  changes

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                                25
from 60 to 1  for forested areas  to  31  to  1  for agricultural areas.
Generally phosphorus Is the limiting nutrient when  the N:P ratio
exceeds 14:1.
    The nutrient loads per unit area of drainage for total phosphorus
and total nitrogen are shown in Figure 9.  The differences  In exports
for the different land use categories are not as pronounced as the
nutrient  concentrations were.  Total phosphorus export from agricul-
tural  lands was  only  3.7  times greater than from forested lands and
total  nitrogen  export only 2.2 times  greater.  The differences in
magnitude between  stream loads and stream  concentrations are due to
 the differences in stream flows  resulting  from  the two types of land
 use.   The data  suggest that stream flow  per unit of  drainage area
 is somewhat higher for forested than for agricultural areas.   This
 seems logical since forested areas frequently are  those  which  are
 unsuitable for agricultural purposes because of steeper  slopes and
 relatively thin soils.
     The  pattern for orthophosphorus concentrations was very similar
 to that  for  total phosphorus as shown in  Figure 10.  Except with
 predominately  urban  drainage areas,  of which their were only eleven,
 mean  orthophosphorus concentrations  represented 40 to 43% of the
 total phosphorus  concentrations regardless  of  overall land use.
 Orthophosphorus concentrations  in streams draining  agricultural areas
 were nearly 10 times the concentrations in streams  draining forested
  areas.

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S3  FOREST


170  MOSTLY[FOREST


52  MIXED


11  MOSTLY URIAH


M  MOSTLY MRK.


91  MRICULTURE
                            TOTAL  PHOSPHORUS  EXPORT
                                        vs
                                   LAND  USE
or tat
S3
170
S2
11
9C
91
I DATA ON if} SUICMAINAGE AREAS IN
CASTEm uxrrto rr«T»«


MOSUY FOREST
MIXED

MOSTLY URIAH
MOSTLY MRK.
•"••* rwm jnrat*
MRICULTURE
*^
| ••' Z

\ 17.4

| 1..4

I 30.1

|22.7

; | 30.*
i i
0 10 20 30 40
KILOGRAMS PER SQUARE KILOMETER PER YEAR
                              TOTAL  NITROGEN  EXPORT


                                   LAND  USE
                              OAT* ON 473 SUBONAINACE AREAS IN
                                  EASTERN UNITED STATES
                                     440.1
                                                                                                 ro
                                                                  7M.6
                                                     630.5
                                                                                  9*2.3
                                        SOO

                          KILOGRAMS  PER  SQUARE KILOMETER PER  YEAR
                                                                                 1000
Figure 9.   The relationship between  total phosphorus and  total  nitrogen,export
             in streams and land  use in  the eastern U.S.                •'•

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or sues
 S3  FOKST

 IT*  MOSTLY FOKST

 S2  MIXED

 11  MOSTLY UMAM

 M  MOSTLY M*K.

 M  ACMCULTUM
             MEAN  ORTHOPHOSPHORUS CONCENTRATIONS
                                   vs
                              LAND USE
                        DATA ON 471 SUBDKAINAGE AREAS 'N
                           EASTfftN UNITED STATES
       J 0.006
                   | 0.014


                       | 0.017
                                               0.033
                                                                                   O.OM
             0.01
                           0.01
                                                       0.04
                                                  PER LITER
                                                                     o.os
                                                                                   0.0*
OF SUIS

 S3   FOKST _

170   MOSTLY FOKST


 52   MIXED


 11   MOSTLY UMUIM

 M   MOSTLY MMIC.


 91   UtKULTURE
                 MEAN INOMANIC NITROGEN CONCENTRATIONS

                               LAND USE
                        DATA ON 473 SUBORAINAGE AHCAS IN
                           EASTERN  UNITED  STATES
       0.131
                                                                                                                rv>
        J 0.347
                  J 0.»7*
                                    I 154
                             J I.O49
                                                                                         J ».l»0
             o.so
                           I.OO
                                                          1.50            3-00
                                                         MILLIGRAMS PER LITER
                                                                     1.50
                                                                                   3.00
Figure  10.  The  relationship between  orthophosphorus  and  inorganic nitrogen,
              concentrations  in streams and land use in the eastern  U.S.

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                                  28
     Inorganic  nitrogen  exhibited quite a different pattern from
 total  nitrogen in  that  substantially higher  (13.7X) concentrations
 were observed  in streams draining agricultural lands than In forested '"
 lands  (Figure  10).   In  streams drainage forested areas, Inorganic nitro-
 gen  constituted about 27% of the total nitrogen, however, this Increased
 to 762 in  streams  draining predominately agricultural areas.  Although
 thp  sample size (11  drainage areas) was relatively small, Inorganic
 nitrogen made  up about  98% of the total nitrogen In streams draining
 mostly urban drainage areas.  Inorganic nitrogen export was also signifi-
 cantly higher  (5.6X) from agricultural areas than from forested areas as
 shown  in Figure 11.  The difference probably reflects the use of inorganic
 nitrogen fertilizers and the high water solubility of inorganic nitrogen
 compounds.
    What conclusions can be drawn from these general  results?  First,
 these  data suggest that streams draining agricultural  watershed have
 higher nutrient levels and therefore would be expected to be more produc-
 tive than those draining forested watersheds.  The Increase in nutrient
 levels  is generally proportional  to the Increasing percent of the land
 in agriculture.
    Second, the data indicate that the inorganic  portion (orthophosphorus)
of the total phosphorus  component stays roughly at the 4035 level  regardless
of land use type, whereas,  the Inorganic  portion  of the total  nitrogen
component increases markedly  from 27%  for forested areas to  75% for

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NMMfR
OF sues

 S3  FOREST

170  MOSTLY FOREST


 52  MIXED


 II  MOSTLY URMN


 M  MOSTLY MRIC.


 tl  AGRICULTURE
OF SU*S

 S3  FOREST
      p'-v •'pr • —v •»,»-«

170  M9STLV FOREST


 52  MIXED


 11  MOSTLY URMH

 M  MSTLY MRK.


 tl  A6RKULTURE
                    ORTHOPHOSPHORUS  EXPORT
                                  vs
                             LAND  USE
                       DATA OH «73 SUBCWAINAGE AREAS IN
                           EASTIRN UNITED STATES
                                             TO
                                                   7.,
                                                          I..,
                               5                               10
                                KILOGRAMS PER SQUARE KILOMETER PER  YEAR
                       INORGANIC  NITR06EN EXPORT
                                  vs
                              LAND  USE
                        DATA ON 47] SUBORAINAGE AREAS IN
                           EASTERN  UNITED  STATES
                                                        ]  S36 9
                                                                                                lS.O
                                                                                     »•>
                   200
                                                           400                   «00
                                                  KILOGRAMS PER SQUARE KILOMETER PER YEAR
                                                                            3 "•.«
                                                                                   800
Figure  n.  The  relationship  between  orthophosphorus  and  inorganic nitrogen
              export in streams and land use in the eastern  U.S.

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                                 30
agricultural areas.  Inorganic nitrogen In streams draining mostly urban
areas represented a substantially larger fraction of the total  nitrogen
(98%), however the number of test areas was relatively small (11).   "•,„
    Lastly, what uses can be made of the data derived from this segment
of the survey?  Other than elucidating the land use-nutrient level -
eutrophication relationships, probably the two most important uses will
be:  (1) to provide a basis for a quick and relatively accurate method
of determining nitrogen and phosphorus concentrations and loadings based
on land use and other non-point source types of geographical character-
istics, and (2) to provide a large nationwide collection of watershed
data for testing other methods of estimating nitrogen and phosphorus
levels in streams from non-point sources.
SUMMARY
    The National Eutrophication Survey, which was Initiated In  1972
by the U.S. Environmental Protection Agency, is In the first stage of
collecting data from over 800 lakes and reservoirs in the contiguous
United States.  In the eastern U.S., a large percentage of the  surveyed
water bodies are impacted by municipal sewage treatment plant effluent
and are in various stages of enrichment.  Phosphorus loads to a signifi-
cant number of these Impacted lakes and reservoirs could be substantially
reduced by controlling phosphorus inputs from municipal  sources.
    Primary production in 67% of the water bodies surveyed east of the
Rocky Mountains was phosphorus-limited and 30% were nitrogen-limited

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                                 31
according to algal  assay results.   It Is  believed that the apparent
nitrogen-limited condition was frequently the result of excessive phos-
phorus inputs from municipal  sources.
    Land use in the watershed was  shown to be a significant  factor in
determining levels of phosphorus and nitrogen 1n streams  in  selected
areas studied in the eastern United States.  Average  total phosphorus
concentrations were about 10 times greater 1n streams draining agricul-
tural areas than in streams draining forested areas;  total nitrogen
concentrations were about five times greater.  The percentage of total
nitrogen 1n the inorganic form was substantially higher in streams
draining agricultural lands than in those streams draining forested
lands.
     Phosphorus loading data for 23 selected  survey lakes were applied
to  three general models  relating annual  total phosphorus loading rates
to  lake  trophic conditions.   The "fit" of observed conditions to pre-
dictions made  by each model was compared and discussed.

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                                 32
REFERENCES CITED

Dillon, P. J.   1975.  The phosphorus  budget of Cameron Lake. Ontario:
    the importance of flushing rate to the degree  of eutrophy of lakes.
    Limnol. Oceanogr.  20_: 28-39.

Larsen, D. P.  and H. T. Mercler.   1975.  Lake phosphorus loading graphs:
    an alternative.  National Eutrophlcation  Survey Working Paper No.
    174.

U.S. Environmental Protection Agency.  1974.  Survey methods for lakes
    sampled 1n 1972.  National Eutrophication Survey Working Paper No. 1.
    40 pp.

U.S. Environmental Protection Agency.  1975.  Survey methods, 1973-1976.
    National Eutrophlcation Survey Working Paper No. 175.  91 pp.

U.S. Environmental Protection Agency.  1974.  An approach  to a  relative
    trophic Index system for classifying lakes  and reservoirs.  National
    EutropMcation Survey Working Paper No. 24.  44 pp.

Vollenweider, R. A.  1968.  The scientific basis of lake and stream
    eutrophlcation with particular reference to phosphorus and  nitrogen
    as factors in eutrophication.  OECD, DAS/CSI/68-27.  159 pp.

Vollenweider, R. A. and P. J. Dillon.  1974.  The  application of the
    phosphorus loading concept to eutrophication research. National
    Research Council of Canada No. 13690.  42 pp.

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