Asbestos in the Water Supplies of
         the Ten Regional Cities
waiter a me crone associates, inc.

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                       Dr. R.J. Carton
                Environmental Protection Agency
                   Office of Toxic Substances
                   Washington,  D.C.  20460

                      Contract 68-01-2690
                 Asbestos in the Water Supplies of

                     the Ten Regional Cities
Date:       22 APril 1976

MA Number:  MA 4200

Copy  /? of  M
        waiter a me crone  associates, inc.
         2820 SOUTH MICHIGAN AVENUE • CHICAGO, ILLINOIS 60616

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-Asbestos in the Water Supplies of
      the Ten Regional Cities


           Summary
           Discussion
           Conclusions

      Appendix 1   Boston
      Appendix 2   New York
      Appendix 3   Philadelphia
      Appendix 4   Atlanta
      Appendix 5   Chicago
      Appendix 6   Dallas
      Appendix 7   Kansas City
      Appendix 8   Denver
      Appendix 9   San Francisco
      Appendix 10   Seattle
      Appendix 11   List of Sample Sites
      Appendix 12   Analytical Methodology
                        waiter c. me crone associates, inc.

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City of Boston
              The Boston water supply originates from two main sources - the
Quabbin watershed and the Wachusett watershed - located in Central Massachu-
setts and is fed to the city by a series of aqueducts.  The city was visited on two
occasions, 25 July 1975  and 17 October 1975 when samples were collected at
Norumbego treatment station situated on the Hultman aqueduct.  In March 1976
additional samples were received from  the city of Boston water department.
These samples were two raw samples taken at the outlets of Quabbin and
Wachusett Reservoirs, a raw sample taken at the Norumbego treatment station
and a chlorinated sample taken at Newton pumping station.
              The results of the analyses for asbestiform minerals are tabu-
lated.  The data for Norumbego on 7/25/75 at first seem anomalous. The
detection of amphibole in the chlorinated water  only is not at present understood.
The amphibole type present could be amosite or crocidolite. (Na is not readily
detectable by the EDXRA (energy dispersive x-ray analysis) system and some
crocidolite standards have failed to give a detectable peak.) The possibility of
amphibole contamination, such as from a gasket or insulation cannot be entirely
ruled out. In connection with a different contract in which considerable amounts
of chrysotile  were detected in a sample the source of this asbestos was traced
to a deteriorating gasket in a pump in the treatment plant of that city.
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Sample location and type

 Norumbego station
              Raw
                          City of Boston
               Date
              7 25 75
              Chlorinated    7 25 75

 Norumbego station
              Raw          10 17 75
              Chlorinated   10 17 75
 Quabbin

 Wachusett

 Norumbego

 Newton
Raw

Raw

Raw
March 76

March 76

March 76
Chlorinated   March 76
               F.p.l.1 (xlO6)
 BDL   (0.28)
 6.7 (C)R
 1.4 (A)2
 4.4 (C)R


 7.5 (C)
10 (C)
 8.1 (C)R
 BDL R (0.126)

 BDL   (0.126)

 BDL   (0.126)

 BDL   (0.126)

 BDL   (0.126)
                    [i g/litre
                                                 0.069
                                                25.2
                                                35.7

                                                 1.43
                                                33.8
                                                22.6
 1) F. p. 1. = fibers per litre
 2) C = Chrysotile;  A = Amphibole; R = Replicate analysis
 3) BDL = Below detection limit; number in parentheses = detection limit
 4) n. d. = none detected
 5) Less than 5 fibers detected
                                         waiter c. me crone associates, inc.

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Sample location and type

 Queen  Lane     Raw
         City of Philadelphia


              Date

             5 1475
 Queen  Lane     Finished      5 14 75
 Belmont
Raw
 Torresdale
5 1475
 Belmont         Finished      5 14 75

 Xorresdale      Raw          5 14 75
Finished     5 14 75
70 (C)2
BDL^R2 (1/0)

BDL3  (0.13)
11 (C) R2

24 (C)2
84 (C) R
6.7 (A) R2

0.75 (C}2

BDL3  (2.5)
200 (C)2 R

17 (Q2
4
                                  Hg/litre
                                    3.89
 0.227

31.9
12.9
 0.322

 0.007
                                    1.233

                                    0.581
                                    0.301
1) F.p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole;  R = Relicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
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Sample location and type

Marston Conduit 20
              Raw
Marston Conduit 30
              Finished
Moffat


Moffat



Marston

Marston


Moffat

Moffat

Moffat
Raw
Finished
Raw

Finished
 blend

Raw
City of Denver


    Date



   2 2675



   2 2675


   2 2675


   2 2675



   9 15 75


   9 15 75

   9 15 75
Finished No. 2   91575
Finished No. 3   91575
                                    1     6
                              F.p.l.  (xlO )
BDL   (0.25)
0.22(A) R(5)


BDL   (0.25)
0.056(A) R(5)

BDL   (0.25)
BDL R (0.1)

BDL   (0.25)
BDL R (0.05)


1.5(5)  (C)
BDL   (0.52)

BDL   (0.50)

BDL   (0.50)

BDL   (0.50)
                    H.g/litre
                                                                 .491
                                                                 .333
                                                   .648
1) F.p.l. = fibers per litre
2) C = Chrysotile;  A = Amphibole;  R = Replicate analysis
3) BDL = Below detection limit; number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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                  Standard Chrysotile Dispersion
                                                  /»
   No.     No. of observations     Fibers/litre (xlO )     Relative Std.dev. (%)
 la              39                     33.5                  9.88
 lb               9                     34.3                 ig.08
 lc               5                     31.7                 38
 Id              15                     25.7                  8.96
 le              15                     27.4                  6.85
 If               5                      2.38                11.09
 lg               5                      0.36                48.6

 Notes
 Runs la - lc freshly prepared dispersion, results of 3 different observers.
 la = 39 observations, 3 grid squares on each of 13 different grids
 lb = 9  observations, 9 grid squares from 5 different grids
 lc = 5  observations, 5 grid squares from 1 grid
 Id and le = 2nd and 3rd preparation from standard after manual agitation to
redisperse,  3 grid squares from 5 grids, observer of la
If = suspension of la - le, diluted 10:1,  1 grid square from 5 grids,  observer
of 1 a
lg = as If, but diluted 100:1
                                         waiter c. me crone associates, inc.

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           Asbestos in the Water Supplies of the Ten Regional Cities

Summary                 As part of a program to determine the impact of point
            and non-point sources on waterborne levels of asbestos, samples of
            raw and finished wafer from the ten cities housing regional head-
            quarters of  the Environmental Protection Agency have been examined.
            These cities are Boston, New York, Philadelphia, Atlanta, Chicago,
            Dallas,  Kansas  City, Denver, San Francisco and Seattle.

                         The results of this examination show that while New
            York, Chicago,  Dallas, Kansas City and Denver are essentially free
            of asbestos, asbestiform fibers have been detected at Boston,
            Philadelphia, Atlanta and Seattle and the potential exists for asbestos
            contamination in the water supply of San Francisco.
                                         waiter c. me crone associates, inc.

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 Introduction
              The Office of Toxic Substances of the Environmental Protection
 Agency has sponsored a nationwide survey to determine the impact of point and
 non-point sources on waterborne levels of asbestos. This survey covers both
.natural sites, in which asbestos.bearing rocks are prevalent, and man-made
 sources.  Additionally,  it provides for sampling water supplies in a number of
 cities and towns.  A listing of these sites is given in Appendix 11 accompanying
 this report.
              This report records the results of the analysis of water from
 the ten major regional cities, headquarters of the EPA regional  offices.
 Sampling and Analysis Methods (See Appendix 12)
              Aliquots of the water were vacuum filtered through 47 mm dia
JMillipore 0.45 (im pore size filters. This filter was then prepared for examina-
 tion on the transmission electron microscope by the direct transfer method.
 Nylon support grids were used to minimize background signals during X-ray
 analysis in EMMA-4, the combined electron microscope microanalyzer.
 Wherever practicable samples for analysis were filtered directly on site.
 Exceptions to this were the cities of Chicago, Denver and Seattle.
 Results
              Appendices 1 -10, numbered to correspond to the EPA region
number, each describe the sampling locations and conditions, tabulate the
results obtained and briefly discuss  the results.
General Discussion
              Although asbestos fibers have been detected in the water sources
and supplies of some major cities, in almost all cases the fiber sizes have been
extremely small.  Indeed in most cases the mean fiber length noted is of the
order of 1.5 to 2 micrometers with some instances of submicrometer mean
lengths observed.  The most marked exceptions to this generality occur in
samples from Boston with mean lengths of 5.4 nm and 9.6 pm but here, other
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 anomalies in the data have led to the postulation of a proximal source for the
 asbestos,  such as a deteriorating gasket or damaged transite pipe.
              The role of climatic changes appears to have been demonstrated
 in Atlanta and Philadelphia where samples associated with high river flow rates
 have shown an increased asbestos  count. Climate might also be expected to
 influence possible asbestos levels  in the San Francisco area since the reservoirs
 of that city which showed an asbestos content (principally Calaveras and Lower
 Crystal Springs) receive water from the Hetch-Hetchy aqueduct during periods
 of low precipitation in their neighboring watersheds and thus are not contributing
 to the water supply at that time.
              In all instances where asbestos has been detected the predominant
 asbestos type has been chrysotile and,  again with the exception of one analysis
 from one Boston sample asbestiform amphibole has not been observed other
 than associated with chrysotile.
              It is necessary to inject a note of caution on the significance of
 the mass per litre data.  This data is derived mathematically from the dimen-
 sions of the individual fibers, assuming a density of 2.3 g/cc for  chrysotile
 and 3.3 g/cc for amphibole and a square cross section. Apart from the geo-
 metric limitations referred to in Appendix 12 it should be borne in mind that
 the occurrence  of a single large, or, more particularlyswide fiber will
 severely skew the data to an unrealistically high figure.
 Conclusions
              Although asbestos fibers have been detected in the water supplies
and sources of five  of the ten regional cities — Boston, Philadelphia, Atlanta,
San Francisco and Seattle — the fiber size observed is generally very small,
averaging approximately 1-3 pm long.  The remaining five cities, New York,
Chicago, Dallas, Kansas City and Denver showed no asbestos levels above
background.
              Some evidence exists for seasonal variations in the observed levels.
It has not been established whether these levels are attributable to natural  or
point sources.
                                         waiter c. me crone associates, inc.

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Appendix 1 Boston
               waiter c. me crone associates, inc.

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City of Boston
              The Boston water supply originates from two main sources - the
Quabbin watershed and the Wachusett watershed - located in Central Massachu-
setts and is fed to the city by a series of aqueducts.  The city was visited on two
occasions, 25 July 1975 and 17 October 1975 when samples were collected at
Weston reservoir, one of the small overflow reservoirs on the aqueduct system.
In March 1976 additional samples were received from the city of Boston water
department.  These samples were two raw samples taken at the outlets of
Quabbin and Wachusett Reservoirs, a raw sample taken at Norumbega reservoir
(similar to Weston reservoir but situated on the Hultman aqueduct) and a chlo-
rinated (presumably finished) sample taken at Newton pumping station.
              The results of the analyses for asbestiform minerals are tabulated.
The data for Weston on 7/25/75 at first seem anomalous.  The detection of
amphibole in  the finished water only is not at present understood.  The amphibole
type present could be amosite or crocidolite.  (Na is not readily detectable by
the EDXRA (energy dispersive x-ray analysis) system and some crocidolite
standards have failed to give a detectable peak.) The possibility of amphibole
contamination, such as from a gasket or insulation cannot be  entirely ruled out.
In connection with a different  contract  in which considerable amounts of chryso-
tile were detected in a sample the source of this asbestos was traced to a
deteriorating gasket in a pump in the treatment plant of that city.
              It should be noted that the March 1976 samples do not duplicate
the 1975 samples in that Norumbego reservoir is not located on the same
aqueduct as Weston and the advisability of additional sampling on both aqueducts
should be considered.
                                         waiter c. me crone associates, inc.

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Sample location and type

 Weston Reservoir
              Raw

              Finished

 Weston Reservoir
              Raw
              Finished
 Quabbin

 Wachusett

 Norumbego

 Newton
Finished

Finished

Finished

Finished
           City of Boston


               Date


               725 75

               7 25 75
              10 17 75
              10 17 75
March 76

March 76

March 76

March 76
               F.p.l.1 (xlO6)
 BDL   (0.28)
 6.7 (C)R
 1.4 (A)2
 4.4 (C)R


 7.5 (C)
10 (C)
 8.1 (C)R
 BDL R (0.126)

 BDL   (0.126)

 BDL   (0.126)

 BDL   (0.126)

 BDL   (0.126)
                    \i g/litre
                                    0.069
                                   25.2
                                   35.7

                                    1.43
                                   33.8
                                   22.6
 1) F. p. 1. = fibers per litre
 2) C = Chrysotile; A = Amphibole; R = Replicate analysis
 3) BDL = Below detection limit; number in parentheses = detection limit
 4) n. d. = none detected
 5) Less than 5 fibers detected
                                         waiter c. me crone associates, inc.

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Appendix 2  New York
                waiter c. me crone associates, inc.

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City of New York



              The city of New York water supply is derived from two main



systems - the Catskill-Delaware system which is fed to the Hillview Reservoir



in Yonkers and the lower level Croton system.  The water is treated with



CuSO. for algae control, chlorinated to maintain a level of 0.8-1.0 ppm free
     4


chlorine and fluorinated.  pH is adjusted to 6.9-7.0 by the addition of NaOH.



              The water was grab sampled at 3 reservoirs on 2 separate



occasions,  11 August and 22 October 1975.  Raw water was sampled at the



Hillview, Jerome Park and Central Park reservoirs and finished water at the



Hillview and Central Park reservoirs. At Hillview, both the Catskill source



and Delaware source raw waters were sampled.



              No asbestiform minerals were detected in any New York City



water samples, either in the raw  water or in the finished product.
                                        waiter c. me crone associates, inc.

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                         City of New York

Sample location and type        Date

Jerome Park Reservoir
              Raw           8 11 75

Central Park Reservoir
              Raw           8 11 75
              Finished       8 11 75

Hillview Reservoir
              Raw           8 11 75
              Finished       8 11 75

Jerome Park Reservoir
              Raw          10 22 75
              Finished      10 22 75

Central Park Reservoir
              Raw          10 22 75
              Finished      10 22 75

Hillview Res(Catskill
 source)      Raw          10 22 75

Hillview Res  (Delaware
 source)      Raw          10 22 75
Hillview Res   Finished      10 22 75
F.p.l.1 (xlO6)
 BDL   (0.18)
 BDL   (0.21)
 BDL   (0.13)
 BDL   (0.36)
 BDL   (0.18)
 BDL   (0.25)
 BDL   (0.25)
 BDL   (0.25)
 BDL   (0.25)
 BDL   (0.25)
 BDL   (0.25)
 BDL   (0.25)
g/litre
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole
3) BDL = Below detection limit; number in parentheses = detection limit,
                              f.p.l.
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 3  Philadelphia
                  waiter c. me crone associates, inc.

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City of Philadelphia
              The city of Philadelphia draws its water supply from two main
sources, the Schuylkill River and the Delaware River via 3 plants - Queen Lane
and Belmont on the Schuylkill  and Torresdale on the Delaware.  The city was
visited on 3 occasions  - 14 May 1975, 27 October 1975 and 1 and 2 April 1976.
On flie first two occasions grab samples were taken at  all 3 plants.  On the
third visit both grab and integrated samples were taken.  Grab samples were
also collected at points on the Schuylkill River and Wissahickon Creek - a
principal tributary of the Schuylkill having its confluence with the Schuylkill just
above the Queen Lane intake.  Samples which had been collected on the Delaware
during boat surveys by the Philadelphia water department were also received
on the third visit.   Only the data for the water supply samples are presented in
this  report.  Data for the river samples will be presented in a later report.
              The results of the analyses are presented in the table following
this  report.  It will be noted that some wide variations occur between replicate
samples.  One explanation for this lies in the appearance of the asbestos observed
which both analysts described as occurring in clumps.  Such clumping generally
makes quantitation more difficult and less objective e. g. it is difficult on
occasion to decide whether a clumped fiber is truly one fiber or an aggregate
of several; additionally, other adhering particulates may obscure fibers or
portions of fibers.
              Despite these difficulties there is no doubt that asbestos fibers
are present in the raw water intakes of Queen Lane and Belmont and that a
portion of these can pass through the Belmont filtration and be released into the
water supply. The situation at Torresdale is less clear.  We believe the high
counts observed at Torresdale may well represent isolated events which are
not reflected in the later, integrated samples.
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             Another confusing factor in the Philadelphia situation is the
weather effect.  On both the 1975 visits the rivers were running above their
long term monthly averages as shown in the following table.
                     Daily Average Flow (cfs)
 Date             Delaware River at Trenton    Schuylkill River at Philadelphia
 51075                  21,400                          3,860
 51175                  18,800                          3,570
 5 12 75                  17,000                          3,360
 51375                  17,900                          4,310
 5 1475	24.700	4.720	
 51575                  26,800                          3,830
 51675                  25,900                          4,510
Long term May avg.       14,600                         23,390
102475                  26,400                          4,860
102575                  22,600                          4,370
102675                  20,100                          4,030
10 27 75	18.000	3.670	
102875                  16,100                          3,280
102975                  15,100                          3,000
103075                  13,700                          2,770
Long term Oct avg          4,200                          1,425

The April 1976 visit was marked by severe thunderstorms and very heavy rain
on the night before sampling.  The decision was therefore made to integrate the
samples over two 12 hour periods  instead of one 24 hour period in order to
determine whether any weather effects could be detected, and indeed we do see
an increase at all three intakes during the second 12  hour session. This is
believed to indicate transport of material from upstream shore line asbestos
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deposits which are more severely eroded during adverse weather conditions.
The exact location of these deposits is not at present identified.
                                       waiter c. me crone associates, inc.

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Sample location and type

 Queen  Lane     Raw
         City of Philadelphia


              Date

             5 1475
 Queen  Lane     Finished      5 14 75
 Belmont
Raw
5 1475
 Belmont         Finished      5 14 75

 Torresdale      Raw          5 14 75


 Torresdale      Finished      5 14 75
   (C)2
   L3R2 (1.0)

BDL3  (0.13)
                70 (C)2
                BDL
                                                 (C)R
24 (C)
                             6.7 (A) R*

                             0.75 (CJ2

                             BDL3  (2.5)
                             200 (C)2


                             17  2
                             4(C)2
Hg/litre

  3.89



  0.227

 31.9
 12.9
  0.322

  0.007


  1.233

  0.581
  0.301
1) F.p..l. = fibers per litre
2) C = Chrysotile; A = Amphibole;  R = Relicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                          waiter c. me crone associates, inc.

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                         City of Philadelphia

                                                  1    6
Sample location and type         Date         F.p.l.  frlO )       tig/litre

 Queen Lane      Raw          10 27 75         BDL3   (0.13)
                                              100 (C)R2           210.54

 Queen Lane      Finished      10 27 75         BDL3

 Belmont         Raw          10 27 75         230 (C)2               2.6

 Belmont         Finished      10 27 75         130 (C)2
                                              26(C)R2              0.588

 Torresdale      Raw          10 27 75         160 (C)2               3.63

 Torresdale      Finished      10 27 75         16 (C)2                0.259
                                              60 (C) R2              0.016
1) F.p.l. = fibers per litre
2) C = Chrysotile; A = Amphibole; R = Replicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                          waiter a me crone associates, inc.

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                    Philadelphia Integrated samples
Sample location and type
 Queen Lane
 Queen Lane
 Belmont
 Belmont
 Torresdale
 Torresdale
 Queen Lane
 Queen Lane
 Belmont
 Belmont
 Torresdale
 Torresdale
Raw
Finished
Raw
Finished
Raw
Finished
Raw
Finished
Raw
Finished
Raw
Finished
 Date

4 176
4176
4 176
4176
4 1 76
4176
4276
4276
4276
42 76
4276
42 76
24
BDL   (0.13)
7.7 (C)2
1.1 (C)2
BDL3  (0.25)
BDL3  (0.13)
120 (C)2
BDL3  (0.13)
50 (C)2
4.3 (C)2
0.745 (C)2
1.0 (C)2
Hg/litre

  0.501

  0.016
  0.075
 22.423

  1.391
  0.213
  0.012
  0.02
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole; R = Replicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                         waiter c. me crone associates, inc.

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Appendix 4 Atlanta
               waiter c. me crone associates, inc.

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 City of Atlanta
              Atlanta draws its water from the Chattahoochee River which rises
 in the hills of White County and flows south to join the Apalachicola and from
 thence into the Gulf of Mexico.
              The city of Atlanta water supply was sampled on two occasions,
 3 April 1975 and 28 November 1975.  On each visit a sample of the raw water
 and a sample of the finished water was obtained from the Atlanta water works
 quality control control center located in Northwest Atlanta.  Two additional
 samples taken by the Atlanta water department in March 1976 were also obtained.
              The results of the analysis for asbestiform minerals are pre-
 sented in the accompanying table.  The presence of chrysotile asbestos during
 the April sample, its absence in November and its recurrence in March of 1976
 may reflect seasonal variations.  It was noted during the April 1975 visit that
 sampling had followed a violent thunderstorm with tornado watch the previous
 evening and that the Chattahoochee River was rising due to torrential rains:
 indeed the flow rate, recorded at 11,300 c.f. s. was almost 2-1/2 times the
 1974 average of 4800 c.f.s.
              At the time of sampling in March 1976 the river was again
 reported as running at a high level.
              At such periods of high flow rate natural erosion processes are
 accelerated and settled detritus may be reentrained.  Additionally  shore line
 deposits, either natural or industrial may become submerged and eroded which
 normally are unaffected by the river flow.  We  believe, therefore, that the
asbestos content of Atlanta raw water may be related to climatological conditions.
                                          waiter c. me crone associates, inc.

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Sample location and type
                            City of Atlanta
                               Date
 Water Quality Control Center
                    Raw        4 3 75

 Water Quality Control Center
                    Finished    4 3 75
                    Finished    4 3 75

 Water Quality Control Center
                    Raw        11 28 75
                    Finished    11 28 75

 Water Quality Control Center
                    Raw        March 76
                    Finished    March 76
                                             8.4 (C)*
                                             12 (C)
                                             11 (C) R
                                             BDL    (0.5)
                                             BDL    (0.12)
                                            36 (C)
                                             BDL
(0.12)
           Eg/litre
             0.119
             0.574
             0.193
             0.256
1) F.p.l. = fibers per litre
2) C = Chrysotile; A = Amphibole; R = Replicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                          waiter c. me crone associates, inc.

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Appendix 5  Chicago
               waiter c. me crone associates, inc.

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City of Chicago




              The city of Chicago draws its water supply from Lake Michigan




via a series of offshore cribs located approximately 1 mile out in the lake.  The




water supply was sampled by Chicago water department personnel on March 27,




1975,  and two samples, identified as "Raw Crib" and "Outlets" were brought




the same day to the Chicago laboratory of Walter C. McCrone Associates, Inc.,




where they were immediately filtered.




              No asbestiform minerals were detected in either the raw or the




finished water.
                                        waiter c. me crone associates, inc.

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                          City of Chicago

                                                 1     6
Sample location and type        Date         F.p.l.  (xlO )       tig/litre
Raw Crib                   3 27 75          DDL  (0.25)


Outlets                      32775          BDL  (0.25)
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole
3) BDL = Below detection limit; number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 6  Dallas
              waiter c. me crone associates, inc.

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City of Dallas



             Samples of the city of Dallas water supply were obtained from




EPA Region 6 personnel (F. Warren Norris, Jr., Water Division), and




were filtered at McCrone Associates on receipt during March 1975. The




samples were identified by EPA codes as 10841-Raw water; and 10842-




Finished water.



             No asbestiform minerals were detected in either sample.
                                       waiter c. me crone associates, inc.

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                          City of Dallas
                                                 l    fi
Sample location and type         Date        F.p.l.   (xlO )       tig/litre
10841    Raw                March 1975      BDL   (0.25)

10842    Finished            March 1975      BDL   (0.25)
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole
3) BDL = Below detection limit; number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 7 Kansas City
                 waiter c. me crone associates, inc.

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Kansas City




              Metropolitan Kansas City has three water treatment plants




serving Kansas City, Kansas; Johnson County, Kansas;  and Kansas City,




Missouri.  All three water treatment plants were visited on September 17,




1975.




              The Johnson County plant supplies water to purchasers in the




communities neighboring Kansas City,  Kansas on the south.  Water from the




Kansas River seeps into 21 wells located along the shore.  It is pumped from




these wells into the water treatment plant for processing.  Samples were obtained




from the Kansas River at the wells  and also from the treated water tap at the




plant.



              Both Kansas City, Kansas and Kansas City, Missouri draw water




from the Missouri River near treatment plants just a few miles from each other.




Raw and finished water were obtained from each of these plants.




              No asbestiform minerals were detected in either the raw or the




finished water from any of the three Kansas City water treatment plants.
                                        waiter c. me crone associates, inc.

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                           Kansas City


                                                 1    6
Sample location and type       Date          F.p.l.  (xlO )        tig/litre
Johnson County Raw          9 17 75          BDL   (2.1 )
               Finished      9 17 75          BDL   (0.48)

Kansas City,  Missouri
               Raw          9 17 75          BDL   (5.7 )
               Finished      9 17 75          BDL   (0.41)

Kansas City,  Kansas
               Raw          9 17 75          BDL   (2.1 )
               Finished      9 17 75          BDL   (0.55)
1) F. p. 1. = fibers per litre
2) C = Chrysotile;  A = Amphibole
3) BDL = Below detection limit; number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 8  Denver
              waiter c. me crone associates, inc.

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City of Denver




             The city of Denver has two water treatment plants deriving




their water from reservoirs in the mountains. The plants are located at




Marston, in S. W. Denver and Moffat in N.W. Denver.  Samples were obtained




at these plants on February 26, 1975, and September 1975.




              Reference to the analysis results in the accompanying table shows




that low levels of fibrous amphibole were detected by one analyst in both the raw




and the finished water of Marston plant sampled on February 2.  To put this




into perspective,  however, it should be noted that these data correspond to




only 2  fibers observed in the raw water sample and 1 fiber in the finished




sample in 10 electron microscope grid squares - a level which could be back-




ground contamination.  Similarly the chrysotile reported for Marston Raw on




September 15th corresponds to the observation of 3 fibers in 40 grid squares.




             We believe, therefore, that the Denver water supply system is




free of asbestos.
                                       waiter c. me crone associates, inc.

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Sample location and type

Marston Conduit 20
              Raw

Marston Conduit 30
              Finished
Moffat

Moffat



Marston

Marston


Moffat

Moffat

Moffat
Raw
Finished
Raw

Finished
 blend

Raw
City of Denver


    Date



   22675



   2 26 75


   2 26 75


   2 26 75



   9 15 75


   9 15 75

   9 15 75
Finished No. 2   91575
Finished No. 3   91575
                              F.p.l.  (xlO6)
BDL   (0.25)
0.22(A) R(5)


BDL   (0.25)
0.056(A)  R(5)

BDL   (0.25)
BDL R (0.1)

BDL   (0.25)
BDL R (0. 05)


1.5(5)
BDL   (0.52)

BDL   (0.50)

BDL   (0.50)

BDL   (0.50)
                    H g/litre
                                                   .491
                                                                 .333
.648
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole;  R = Replicate analysis
3) BDL = Below detection limit; number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 9  San Francisco
                  waiter c. me crone associates, inc.

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City of San Francisco
             The San Francisco Water System is extremely complex,
deriving its main feed from the Yosemite Mountains some 100-150 miles east
of the city. From the Yosemites the water is fed via tunnels and pipes (The
Hetch-Hetchy aqueduct) to a number of holding reservoirs which are also fed
by local run off.  The map accompanying this report shows, in part, the
complexity of the system.  A "short narrative" (some 22 pages  and 16 pages
of Appendices) describing the San Francisco water suppling system is  available
from the San Francisco Water Department.
             The water system was sampled on two occasions, 5 March 1975
and September 10 and 12,  1975 when the following samples were taken.
    •March 5      Alameda East Portal ("Terminus" of the Hetch-Hetchy
                 aqueduct.)
                       This water has been chlorinated and is regarded
                       as "finished" water. No treatment  other than
                       chlorination is applied.
                 Calaveras Reservoir
                       Surface water sampled approximately 50 yards
                       from boathouse
                 San Antonio Reservoir
                       Surface water sampled at boathouse
                 Lower Crystal Springs Reservoir
                       Surface water sampled at shore line - rock
                       samples also collected - bedrock extremely
                       friable
                 San Andreas Reservoir
                       Faucet on side of chlorination plant -"raw water"
                 San Andreas  filtration plant, outlet no.  1, "finished water"
                                        waiter c. me crone associates, inc.

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                  Pilarcitos Reservoir
                       Surface water

    September 10  Alameda East Portal ("Terminus" of the Hetch-Hetchy
                  aqueduct.)
                       This water has been chlorinated and is regarded
                       as "finished" water.  No treatment other than
                       chlorination is applied.

                  Calaveras Reservoir

                  San Antonio Reservoir

                  Sunol filtration
                       San Antonio treated

                  Crystal Springs
                       "Raw water"

                  Crystal Springs
                       "Finished" -  chlorination only

                  San Andreas Reservoir

                  San Andreas filtration plant, outlet no. 1
                       "Finished" water

                  San Francisco tap water - Airport Holiday Inn

    September 12  Mocassin Reservoir - about 100 miles east of San
                  Francisco


              During analysis of the first batch of samples it became apparent

that sample preparation  problems were being experienced.  The data reported

for these samples, therefore, is generally that obtained by Murchio.
              Sampling  during the second visit was carried out by a different

sampling team and also under a different guide, thus there are some differences

in location at the reservoirs  sampled.  The most noticeable difference in

results between the two samplings is in the Lower  Crystal Springs Reservoir
                     c
samples where 620 x 10  chrysotile fibers were found on the first visit and
                                        waiter c. me crone associates, inc.

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none on the second visit.  We believe this may be explained by the different

locations at which the samples were taken.  It was noted in the trip report for

the first visit that the bedrock adjacent to the sampling location is extremely

friable and samples of this rock were taken.  These samples did indeed turn

out to have a large serpentine content (determined by light microscopy) and the

rock could be powdered between the fingers.  It is therefore not surprising that

the chrysotile content of the first  sample is high.  It might also not be unexpected

that a sampling point some miles  distant from the first should show a com-

pletely different picture.  This highlights the problem of trying to characterize

a system as complex as that of San Francisco which could well merit a major

study of its own.  An additional explanation of differences between the March

and September samples may be found in the San Francisco Water Department

booklet which, in talking of the Crystal Springs, San Andreas, Pilarcitos

section says .... "Historically this has  been a problem water due to high tur-

bidity during the winter months  ..." thus seasonal variation may also be a
factor.

             We believe  the San  Francisco situation may best be summarized

as follows:

             1)   Water from the Hetch-Hetchy aqueduct entering the
                  San Francisco  water system (at Alameda East Portal)
                  is free of asbestos.

             2)   The potential for contamination of the San Francisco
                  water system  by asbestos - both serpentine and
                  amphibole -  exists due to natural sources in certain
                  of the holding reservoirs, principally Crystal Springs
                  and Calaveras  and to a lesser extent San Antonio and
                  San Andreas.

             3)   These natural sources may be isolated rock outcrops
                 whose total impact on a large reservoir (e. g. Crystal
                 Springs holds 22,580 x 10  gallons) may not be signi-
                 ficant.
                      /
             4)  No evidence has been found of asbestos in any of the
                 finished waters examined.
                                         waiter c. me crone associates, inc.

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           GOLDEN
            	UC.fOWJI UXOLlt T«M«» »
            \ CM 100.000 •*•
            \ CM«IM
                                                                STORAGE REEERVCWS

                                                                BISTRIBUIIN5 RESERVOfl
                                                              •  SPARLMG  METERS
a TANK ELEVATIONS	CITY BASE
BAY DIVISION STRUCTURES	U.S.G.S.
      8.74 =__.   	U.S.G.S.
,'^toijj^	«.•>•«• •»••«' ,

i      ^SSWSWWIfllR'M."*"*1™1
                                                                                  SUPERSEDES-X-838-1
                                                                                  ORIGINAL- Jonuory-1949
                                                                                  REVISED-JULY 1973
                  SAN  FRANCISCO  WATER  DEPARTMENT
                                                 X-838-C

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                          City of San Francisco
                                                   1    fi
Sample location and type          Date         F.p.l.  (xlO )
 Alameda East Portal
               Finished

 Calaveras Reservoir
               Raw

 San Antonio Reservoir
               Raw
 Crystal Springs Reservoir
               Raw
                               3 5 75
                               3 5 75
                               3 5 75
                               3 5 75
San Andreas Reservoir
               Raw

San Andreas Outlet No. 1
               Finished

Pilarcitos Reservoir
               Raw
                              3 5 75


                              3 5 75


                              3 5 75
6DL   (0.22)
45 (A)  , 240(C)
0.56(A)5, 0.56(C)5
4.3(A),  180(C)

1.7(A)?  71(C)R



4.1(A)


6DL   (0.05)


BDL   (2.5)
                                                                 ng/litre
                   39.019 A,
                    1. 405 C

                    0.33A,
                    0.13C
                   0.14 A,
                   141 C
                   0.341 A,
                   1.385 C
                   6.74 A
1) F.p.l. = fibers per litre
2) C = Chrysotile; A=Amphibole; R = Replicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                          waiter c. me crone associates, inc.

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                          City of San Francisco
                                                    1    6
 Sample location and type         Date         F.p.l. (xlO )
Alameda East Portal
               Finished        9 10 75

Calaveras Reservoir
               Raw            9 10 75
San Antonio Reservoir
               Raw            9 10 75

Sunol Filtration ( San
 Antonio finished)                9 10 75

Crystal Springs Reservoir
               Raw            9 10 75

Crystal Springs Reservoir
               Finished         9 10 75

San Andreas Reservoir
               Raw            9 10 75

San Adnreas Outlet No. 1
               Finished         9 10 75

San Francisco tap water
               Finished         9 10 75
Mocassin Reservoir
               Raw             9 12 75
 BDL (0.43)


 2.6 (C)5
15 (C) R


 BDL (0.55)


 BDL (0.31)


 BDL (0.48)


 BDL (0.52)


 BDL (0.52)


 BDL (0.55)


 BDL (0.32)
 1. 6 (C)1
                   UgAitre
0.214
0.143
0.04
1) F.p..l. = fibers per litre
2) C = Chrysotile;  A = Amphibole; R = Replicate analysis
3) BDL = Below detection limit; number inparentheses = detection limit
4) n.d. = none detected
5) Less than 5 fibers detected
                                          waiter c. me crone associates, inc.

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Appendix 10 Seattle
                waiter c. me crone associates, inc.

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City of Seattle

           Only 1 sample from the city of Seattle has been examined.  This
sample came from the Tolt Reservoir and is therefore raw water.  The only
further treatment to which this water is subjected prior to passing into the
distribution system is chlorination.  The sample  should therefore be
representative also of the finished product.
           The  "pedigree" of the sample is as  follows:
           EPA Region 10 Drinking Water Programs Branch is actively
conducting a survey program in their region which includes the Seattle
water supply.  Jack Murchio,  a consultant on this project is also associated
with the Seattle project and permission was received from EPA  region 10 for
the transfer of this sample from their project.  The sample is part of a
limited "round robin" series and had been analyzed by two other laboratories
as well as by Murchio.
           The data presented herein is that of both McCrone Associates
and of Murchio,  the latter identified by the final letter 'M1. The samples
designated DM are duplicates run at McCrone Associates on the actual filter
analyzed by Murchio. It will be noted that Murchio reports both amphibole
                                              fi            fi
and chrysotile fibers present at levels of 1.9 x 10  and 1.5 x 10 , respec-
tively, whereas McCrone finds both below the limit of detection.  Attention
is drawn, however, to the McCrone descriptions which mention poorly
diffracting fibers resembling chrysotile but with elemental compositions
rich in Al, Si, Ca, Fe and some Mg.  A positive identification of these fibers
has not been made and the possibility that they are a fine amphibole in the
Tremolite-Actinolite  series cannot be ruled out.  We have examined
Murchio's data and consider that his identification of the chrysotile is valid.
We therefore believe that both chrysotile and amphibole asbestos are pre-
sent in Tolt reservoir water.
                                      waiter c. me crone associates, inc.

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          For further information on the EPA Region 10 program

contact:   Roy Jones, U.S. E.P.A.
          Drinking Water Programs Branch
          1200 6th Avenue
            M/S 429
          Seattle, Washington 98101  Phone:  206 442 1223
                                   waiter c. me crone associates, inc.

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                          City of Seattle


Sample location and type        Date
                     1    fi
               F.p.l.  (xlO )       tig/litre
Tolt reservoir
'9 875
BDL   (0.25)

1.9 (A)M

1.5 (C)M

BDL   (0.25) DM
                                                                1.21

                                                                0.008
1) F. p. 1. = fibers per litre
2) C = Chrysotile; A = Amphibole;  M = Murcbio date; DM = Duplicate run at
                                      McCrone Associates on Murcbio sample
                                      (see text)
3) BDL = Below detection limit;  number in parentheses = detection limit
4) n. d. = none detected
5) Less than 5 fibers detected
                                        waiter c. me crone associates, inc.

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Appendix 11  List of Sample Sites
                     waiter c. me crone associates, inc.

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Appendix 11 -   Sampling areas and expected source types
        Source type
        Natural
        Mining
        Milling
        AC Pipe
        AC Sheet
        Asbestos paper
                        Sampling area
                    Montana - Red/Beaver head Rivers
                    California - Trinity River and various
                    reservoirs in Northern California
                    Vermont -  Connecticut River
                    Tennessee-Georgia - Hiwasee, Little
                    Tennessee  and Upper Savannah Rivers
                    G.A.F. - Vermont

                    Union Carbide - King City, California

                    J.M.*-Dennison, Texas
                    CAPCO - Ragland, Alabama
                    CAPCO - Van Bur en, Arkansas
                    Certain Teed - St. Louis, Missouri
                    Flintkote - Ravenna, Ohio

                    National Gypsum - New Orleans, Louisiana
                    G.A.F. -St. Louis, Missouri
                    J.M.- Nashua,  New Hampshire
                    Nicolet - Ambler, Pennsylvania
                    J. M. - Tilton, N. H.
                    J. M. - Pittsburg, California
                    Armstrong - Fulton, New York
                    G.A.F. -Erie, Pennsylvania
* J. M. = Johns Manville
                                   waiter c. me crone associates, inc.

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Asbestos paper,
  con't.              G.A.F. -Whitehall, Pennsylvania
                    Hollingsworth and Vose - East Walpole,
                     Massachusetts
Millboard           J. M.  - Billerica, Massachusetts

Roofing             G.A.F.  - Erie, Pennsylvania

Tile                Armstrong - Kankakee, Illinois

Misc. (frictionmaterials,
tGXtilGS GtC )
          '         Raybestos Manhattan - Stratford,  Connecticut
                    Raybestos Manhattan - North Charleston, S. C.
                    Raybestos Manhattan - Marshville, N. C.

Multi product        J.M.  - Manville, New Jersey

Accessory minerals  Morenci Mine - Arizona
                    Homestake Mine - South Dakota
                    W.R.  Grace - Montana
                    International Talc Company - New York
Water supplies      10 regional cities
                    Boston
                    New York
                    Philadelphia
                    Atlanta
                    Chicago
                    Dallas
                    Kansas City
                            waiter c. me crone associates, inc.

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Water supplies
  con't.              Denver
                    San Francisco
                    Seattle

The following 7 are associated with potential natural sources
                    lima, Montana
                    Dillon, Montana
                    Weaverville, California
                    Redding, California
                    Chattanooga, Tennessee
                    Anderson,  South Carolina
                    Greenville, South Carolina

The following 13 are finished water at plants where city water
is used in processing
                    St. Louis,  Missouri
                    New Orleans,  Louisiana
                    Erie, Pennsylvania
                    Pittsburg,  California
                    Lead,  South Dakota
                    Billerica, Massachusetts
                    Manville,  New Jersey
                    Nashua, New Hampshire
                    Stratford, Connecticut
                    North Charleston,  South Carolina
                    Marshville, North Carolina
                    Kankakee,  Illinois
                    Van Bur en, Arkansas
                            waiter c. me crone associates, inc.

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Appendix 12  Analytical Methodology
                      waiter c. me crone associates, inc.

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Appendix 12         Method for Determination of Asbestos

           Samples are filtered onto 0.45/xm pore size membrane filters.
Sections of the filter approximately 2-3 mm square are placed face-down on
previously carbon-coated electron microscope grids and the membrane filter is
dissolved,  using acetone,  in a Soxhlet extractor.  Previous work has shown us
that there is very little risk of contamination in transferring the filter on the
electron microscope grid to the Soxhlet extractor.  Furthermore, by dissolving
the filter in situ on the grid, the risk of losing portions of the sample is minimal.
Techniques involving transfer of a liquid suspension directly to the electron micro-
scope grid are more subject to error since there is frequently a size separation
as the meniscus of the drying drop recedes.
           The sample grids are examined on the electron microscope (JEM 200
or EMMA 4) using a magnification such that the intermediate lens aperture is in
focus in the specimen plane.  It is thus possible, by inserting the aperture and
switching to the diffraction position, to obtain a selected area electron diffraction
(SAED) pattern of the fiber with no other adjustments to the microscope.  In this
way it is possible to spot check the diffraction pattern of individual fibers very
rapidly.
           The length and width of each asbestos  fiber is recorded.  Interpolation
from intervals scribed on the screen allows an accuracy of measurement on the
screen of approximately +0.05 cm.  This corresponds to an accuracy in size measure
ment of about  0.02-0.03 /zm.  Measurements of the individual fibers are computer
processed to give listings of the length  and width of the fibers, together with a
computed mass of each fiber computed  on the basis of density, D,  and dimensions,
L and W (D x L x W2).  A value of 3.40 is taken as the mean density of grunerite
amphibole fibers.  Densities of 2.3 and 3.0 are used for chrysotile and tremolite,
respectively.  Because many of the amphiboles are lath-shaped rather than square
                       s
in cross  section, this figure may well be slightly high,  since the laths will,  in
general,  tend to lie flat rather than on edge. There is, however, a finite possi-
                                          walter c. me crone associates, inc.

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bility that some laths will be on edge and, due to the very small size of many
of the fibers of interest, the approximation to a square fiber will not give more
than a slightly high bias to the mass readings.  The program automatically
assigns the longest dimension  to the fiber length and excludes all fibers with an
aspect ratio below three.
           Also presented in the computer printout are the calculated number
of fibers per unit volume, the  calculated mass of fiber per unit volume,  the size
distribution of the fibers based on length and width, and the distribution of fibers
by aspect ratio together with the relevant statistical information on these para-
meters.
           A sample computer printout is attached.

           As mentioned in the  first paragraph, losses in a correctly operated
Soxhlet extractor are minimal, but careful control of both heating rate and cooling
rate of  the  cold finger  are required.  When  these conditions are obtained, and
maintained, estimated losses  (based on filtration and examination of the solvent
after several weeks use) are less than 1 fiber per grid processed.  Additional
evidence that losses are minimal is provided by examining filter segments which
do not fully cover the grid.  These show a clean junction between membrane and
non-membrane area with no evidence of "bleed-off1 of particulates.
           The reproducibility of the method is clearly of prime importance.
A report is currently being prepared describing tests performed to assess the
reproducibility,accuracy and statistical validity of the method.  The accompanying
table presents part of this data and shows that, for a standard chrysotile dis-
persion, the filtration technique,  and the electron microscope preparation method
yield uniform dispersions over the entire filter  area and grid area within a
standard deviation of less than 10%.  This standard deviation, however,  also
includes the variation in the operator's objectivity in deciding whether fiber
bundles are  one fiber or a composite of fibers, thus the S.D. of the dispersion
                                          waiter c. me crone associates, inc.

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is probably better than 10%.  The standard deviation deteriorates to approximately
20% when 3 operators  (using 2 different Soxhlet extractors in different laboratories)
are involved.
            The data also show exceptionally good agreement between expected
and observed values when successive dilutions of 10:1 and 100:1 are analyzed,
although with low loadings the standard deviation again increases, as might be
expected - at extreme dilutions clearly one enters the realm of probability statistics
and the chance of finding a fiber in any given grid square predominates.
            Errors in estimating the fiber content of real samples generally err
on the low side because of practical difficulties such as fiber  overlap - either with
other fibers or with non-fibrous particulates in the sample.  Furthermore,  the
philosophy has been adopted of only counting fibers which can be positively identi-
fied as asbestos during examination of the sample  -  it is impractical to photograph
each fiber together with its diffraction pattern for  later analysis and measurement.
The possibility exists, therefore, that fibers in the grey area between positive
acceptance and positive rejection as asbestos  will  be excluded although they might
be asbestiform.
                                           waiter c. me crone associates, inc.

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                   Standard Chrysotile Dispersion

                                                   /»
Run No.    No. of observations      Fibers/litre (xlO )    Relative Std. dev.
 *a              39                     33.5                  9.88
 lb               9                     34.3                 19.08
 !c               5                     31.7                 38
 ld              15                     25.7                  8.96
 !e              15                     27.4                  6.85
 1*               5                      2.38                H.09
 1 g               5                      0.36                48.6

 Notes
 Runs la - Ic freshly prepared dispersion,  results of 3 different observers.
 la = 39 observations, 3 grid squares on each of 13 different grids
 lb = 9 observations, 9 grid squares from 5 different grids
 Ic = 5 observations, 5 grid squares from 1 grid
 Id and le = 2nd and 3rd preparation from standard after manual agitation to
 redisperse, 3 grid squares from 5 grids, observer of la
 If = suspension of la - le,  diluted 10:1, 1 grid square from 5 grids, observer
 of la
 Ig = as If, but diluted 100:1
                                         waiter c. me crone associates, inc.

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              SAMPLE
                                      
  FIBER CONCENTRATION BY NUMBER,PER LITER
  FIOER CONCENTPATION BY MASS, PER LITER  :
  VOLUME FILTERED :     2.7 ML.
  GPID SQU/iRES COUNTED .  -  16
  TOTAL SUCPEHDED COLIDS:       44.000 MG  PER  LITER
  PM =   7.4
                                      0.14E+B9
                                    1272.500 GRAMS*10+-6
                        DESCRIPTIVE STATISTICS

                           NO. OBS. =     34
  VARIABLE
1 LENGTH
2 WIDTH
3 ASPECT RATIO
4 MASS
               MEAN
           0.32?71E+01
           0.45912E+GO
           0.10909E+02
           0.30250E+01
             VARIANCE
           0.54009E+01
           0.22Q26E+QO
           Q.5612SE-i-G2
           0.42L18E+03
                STANDARD
                DEVIATION

               0.23240E+01
               0.46932E+00
               0.74917E+01
               0.20717E+02
                  STANDARD
                   ERROR

                 O.39856E+Q0
                 0.8Q4S7E-01
                 0.12S4SE+01
                 0.3552?E+01
    1
    2
    3
    4
  SKEUNESS

  14010E+01
  15926E+01
  15959E+01
0.2827-JE+01
0.
0.
0
0.
KURTOSIS

79?31E*CO
16288E+01
2085SE+01
     MAX

0.90000E+01
Q.180GQE+01
0.36000E+02
0.96228E+02
    niN

0 60000E+00
0.5000CE-01
0.33333E+01
0.12700E-01
0.
0.
o!
 RANGE

84000E+01
17560E+01
32667E+02
                                     0.9G215E+02

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          SHHPLC
                  CAMPHIBQLE)
            LENGTH
WIDTH
ASPECT
RATIO
MASS
  1
  2
  7
  4
  5
  6
  7
  8
  9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
1 . 8000
2.2000
3. 4000
3. 0000
9. 0000
1. 4000
2. 0000
2. 0000
2. 4000
4. 0000
4. 0000
2. OCOO
2. 8000
3. 5000
6.0000
0.6000
2. 4000
3. 0000
2. 5000
1 . 8000
2.4300
7. 0000
7. OOBO
2.2600
9. 0000
2. 2000
1.8000
1. 8000
0. 6000
3.50CO
9. eoeo
2.0000
2. 0000
1 . 8000
0. 1009
0. 2080
0. 4000
0. 3000
0.7000
0. 2000
0. 3000
0. 3000
0. 4000
1. 2000
0. 5000
0. 3000
0. 30CO
0. 3000
1. 8000
0. 1000
0. 2000
0. 3000
0 3000
C. 0500
0. 1000
1 . O&OO
1. 3600
0.0800
1. 8000
0. 1000
0. 2COO
0 3000
0. OGOO
0. 6000
1. 0000
0. 6000
0. 1000
0. 1000
18.0900
11. 0000
8. 5000
10. 0000
12.8571
7. 0000
6.6667
6. 6667
6.0000
3.3333
8.0000
6. 6667
9 3333
11. 6667
3.3333
6.0000
12. 0000
10. OCOO
8.3333
36.0000
24. 0006
7. 0000
5. 3846
27. 5000
5. 0000
22. 0000
9.0000
6 06013
7.5600
5.8333
9. OOOO
3. 3333
20. 0000
18. 0000
                                   1.
                                  64.
                    0.0594
                    0.2904
                    1.7952
                    0.8910
                    14.5530
                    0.1848
                    0.5940
                    0. 59-10
                    1.2672
                    19.60SO
                    3.3000
                    0.5940
                    0.8316
                       0395
                       1520
                    0.0193
                    0.3168
                    0.8910
                    0.7425
                    0.0148
                    0.07?2
                   23.1000
                   39.0390
                    0.0465
                   96.2280
                    0.0726
                    0.2376
                    0  5346
                    0.0127
                    4.1580
                   29.7000
                    2.3760
                    0.0660
                    0.0594

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                        SAMPLE
   LENGTH
              DISTRIBUTION BY LENGTH

               NUMBER      PERCENT
0.0
8.5
1.0
1. 5
2. 0
2. 5
3. 0
3.5
4. 0
4. 5
5. 8
5. 5
6. 0
6.5
7. 0
7. 5
8. 0
8. 5
3.0
0. 5
1. 0
1. 5
2. 0
2. 5
3. 0
3. 5
4. 0
4. 5
5. 0
5. 5
6. 0
6. 5
7. 0
7. 5
8. 0
8. 5
3. 0
3. 5
 0
 2
 1
16
 7
 3
 3
 2
 0
 0
 0
 1
 0
 2
 0
 0
 0
 3
 0
 0.00
 5. 88
 2. 94
23. 41
20.53
 8. 82
 8. 82
 5.88
 0. 00
 0. 00
 0. 00
 2. 94
 0. 60
 5. 88
 0. 00
 0. 00
 0. 30
 8. 82
 0. 00
                                           CUMUL&TIVC
                                             PERCLMT

                                               0. 00
                                               5.8S
                                               8.82
                                              38. 24
                                              58. 82
                                              67.65
                                              76.47
                                              82.35
                                              82.35
                                              82.35
                                              82.35
                                              85.29
                                              85. 29
                                              91. IS
                                              91. 18
                                              91. 18
                                              91. 18
                                             100.00
                                             100.00
    WIDTH
              DISTRIBUTION BY  WIDTH

               NUKBER      PERCENT
0. 0
0. 1
0. 2
0. 3
0. 4
0. 5
0. 6
0. 7
0.8
&. 3
1'. 0
1. 1
  2
  3
  4
  5'
1. 6
1. 7
1.8
0. 1
0. 2
0. 3
0. 4
0. 5
0. 6
0. 7
0. 8
0. 9
1. 0
1. 1
1. 2
1. 3
1. 4
1. 5
1. 6
1. 7
1. 8
1. 9
                 3
                 4
                 3
                 2
                 1
                 3
                 0
                 1
                 0
                 0
                 2
                 0
                 1
                 1
                 0
                 0
                 0
                 0
                 2
             26. 47
             11. 76
             26. 47
              5. 88
              2. 94
              8. 32
              0. 00
              2. 34
              0. 00
              0. CO
              5. 88
              0. 00
              2  94
              2. 94
              0. 00
              6.00
              @. 00
              0. 00
              5. 88
               CUMULATIVE
                 PERCENT

                  26.47
                  38.24
                  64.71
                  70.59
                  73.53
                  82-. 35
                  82.35
                  85. 29
                  85. 29
                  85.29
                  31. 18
                  31. 18
                  34'. 12
                  37.06
                  97. 06
                  37. 06
                  37. 06
                  37.05
                 102.94

-------
                   DISTRIBUTION DY AT.rfTCT RCiTIO

ASPECT RATIO          NUMBER         PERCENT     CUMULATIVE
                                                   PERCENT

   3     10             23             67.65         67.65
  10     20              7             20. 59         88 24
  20     30              3              8.8£         97.06
  30     40              1              2. 94        160 OCi
  40     50              0              0.00        100.00
  50     60              0              0.00        100.00
  60     70              0              0.00        100.00
  70     80              0              0.00        100.00
  80     90              0              0.00        100.00
  90    100              0              0.00        100.00
 100    110              0              0.00        100.03
 110    120              O              0.00        lOO.OO
 120    130              O              0.00        100.00
 130    140              0              0.00        100 00
 140    150              0              O.OO        100.00
 150    160              0              0.00        100.00
 160    170              0              0.00        100.00
 176    IRQ              0              0.00        100.00
 18&    196              0              0.00        100.00
 190    200              O              0.00        lOO.OO

 * OVER 230              0              0.OO        100.00

-------