v>EPA
             United States
             Environmental Protection
             Agency
             Office of
             Toxic Substances
             Washington DC 20460
EPA-560/13-80-23
August, 1980
             Toxic Substances
Asbestos -
Containing
Materials
in School
Buildings:

Bulk
Sample
Analysis
Quality
Assurance
Program

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                                          EPA 560/13-80-23
                                              August 1980
    ASBESTOS-CONTAINING MATERIALS
    IN SCHOOL BUILDINGS: BULK SAMPLE
ANALYSIS QUALITY ASSURANCE PROGRAM
                        By

              E. P. Brantly, Jr., and D. E. Lentzen
                 Research Triangle Institute
                  Research Triangle Park
                  North Carolina 27709
                Contract No. 68-01-5848

               Task Manager: Cindy Stroup
               Project Officer: Joseph Carra
               Design and Development Branch
                Survey and Analysis Division
            Office of Pesticides and Toxic Substances
               Environmental Protection Agency
                 Washington, DC  20460

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                                  Disclaimer
     This document has been reviewed and approved for publication by the
Office of Testing and Evaluation, Office of Pesticides and Toxic Substances,
U.S. Environmental Protection Agency.   Approval does not signify that the
contents necessarily reflect the views and policies of the Environmental
Protection Agency, nor does the mention of trade names or commercial
products constitute endorsement or recommendation for use.

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                                   CONTENTS

                                                                          Page

1.    Introduction	1

2.    Procedures	2

3.    Results	3

4.    Plans	4

Appendixes:

Appendix A:  Instructions and Reporting Form  	  5
Appendix B:  Reference Reports  	  9
Appendix C:  Reports to Laboratories  	 14
Appendix D:  Commercial Laboratories  	 16
Appendix E:  Laboratory Data	20
                                      11

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              ASBESTOS-CONTAINING MATERIALS IN SCHOOL BUILDINGS:
                BULK SAMPLE ANALYSIS QUALITY ASSURANCE PROGRAM
1.    INTRODUCTION
     Growing public concern with the effects of exposure to asbestos fibers
has resulted in a greatly increased demand for laboratory analyses to deter-
mine the content of bulk insulation samples.  In the course of the Environ-
mental Protection Agency asbestos-in-schools program, many differences have
been noted in laboratory analytical services contracted for by public school
systems.  Discrepancies among laboratories may be attributed to variations in
analytical methods, lack of appropriate reference standards, and inadequate
reporting of analytical results.
     Polarized light microscopy (PLM) is the EPA method of choice* for detecting
asbestos in bulk insulation samples.  To facilitate the public's contact with
PLM service laboratories, a list of commercial laboratories claiming capability
in PLM analysis was made available to EPA regional asbestos coordinators,
state asbestos contacts, and to the public upon request via a toll-free number
established to provide technical information on sampling and analysis of
insulation materials.
     A quality assurance (QA) program was initiated to qualify, to a limited
extent, the services provided by the listed commercial laboratories.  All
laboratories listed were invited to participate in the QA program.  Laboratories
accepting were provided with four characterized samples and their analytical
reports compared with reference analyses.  This was not an accreditation
program and did not seek to certify or endorse participating laboratories.  A
performance rating based on a fairly lenient criterion was determined for each
laboratory and was included on the updated laboratory list.  Laboratories had
been notified at the start of the project that such a rating would be made.
Participation in the program was required for laboratories to be included on
the final published listing.
     Noncommercial laboratories identified by EPA regional officials, includ-
ing state health departments and universities, were offered the same samples
     *Asbestos-containing materials in school buildings:   A guidance document,
EPA/OTS #C00090, March 1979.

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and reporting forms.  Participation by noncommercial laboratories was also
voluntary and their results will not be available to the public.
2.   PROCEDURES
2.1  Reporting Form and Instructions
     Instructions and a reporting form developed for laboratory use in communi-
cating analytical results are included as Appendix A.  The form satisfies
various information needs, including identity of asbestos and nonasbestos
fibers, analytical method(s) used, quantisation procedures used, and quality
control measures employed.
2.2  Selection and Distribution of Samples
     Four bulk samples were sent to each laboratory.  Two contained asbestos
fibers, anthophyllite and chrysotile, and two were nonasbestos fiber material,
mineral wool and fiberglass, commonly found in insulations.   The samples were
doublebagged, coded, and packaged with a reporting form and instructions for
analysis.  Sample packages were mailed on December 28, 1979, to all laboratories
then on the listing.
2.3  Reference Reports
     Analytical reports were obtained from two reference laboratories, Battelle
Columbus Laboratories of Columbus, Ohio, and the Bureau of Mines, U.S. Depart-
ment of the Interior, Avondale, Maryland.   These are included as Appendix B.
Reference report data were the basis for comparison in determining a laboratory's
performance rating and were included on the reports to laboratories.
2.4  Quantitation
     Estimates were provided by the laboratories of the relative amounts of
sample constituents.  These were averaged for each sample lot, disregarding
errors in fiber identification.   Means and standard deviations were included
on reports to the laboratories.   Because of the lack of an accepted quantita-
tion procedure, values reported were not used in rating laboratory performance.
     The distributions of quantitative estimates were recorded on histograms
in 5 percent intervals.   The histograms were included on individual reports to
allow laboratories to place themselves within the distribution.

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2.5  Reports to Laboratories
     Reports were issued to individual laboratories on March 25,  1980 (commer-
cial), and April 3, 1980 (noncommercial).   Reports included the results of
reference analyses, data reported by the individual laboratory, and summary
data on quantitative estimates.   An example of the reports to laboratories is
included as Appendix C.
2.6  Performance Rating
     Laboratory performance was assessed on the basis of correct identification
of "positive" and "negative" samples.   Reductions of the all-correct (4/4)
score were made only for the reporting of false positives (nonasbestos sample
reported as containing asbestos) or false negatives (no asbestos  reported in
an asbestos-containing sample),  and not for the misidentification of individ-
ual asbestiform mineral species.  The criterion, though lenient,  recognizes
the basic concern of the public—whether or not asbestos fibers are present in
a submitted sample.  Environmental Consulting and Testing Services and Prince-
ton Testing Laboratories were assigned scores of 3/4.  All other commercial
laboratories scored 4/4.
3.    RESULTS
     Seventy-one percent of the laboratories contacted reported results,
including 52 of 72 commercial labs and 23 of 34 noncommercial labs.  Results
included were received on or before January 25, 1980.  Participating commercial
laboratories are listed in Appendix D.  A listing of reported results (fibrous
materials only) is included as Appendix E.
     For the 300 (75 x 4) samples analyzed, no false negatives and only two
false positives were reported.  Environmental Consulting and Testing Services
of Cherry Hill, NJ, and Princeton Testing Laboratory of Princeton, NJ, incorrec-
tly identified mineral wool as crocidolite and amosite, respectively.  All
other laboratories identified the sample as either mineral wool,  fiberglass,
or glass wool.  Although the distinction between these materials  is apparent
to an experienced analyst, it was not deemed important to the present evaluation
program.
     Anthophyllite-asbestos was frequently misidentified as either amosite (15
labs) or tremolite (10 labs).  This was most likely due to unfamiliarity with
anthophyllite-asbestos because no standard reference samples exist and it is
not commonly found in insulation materials.  Closer attention to  extinction
                                      3

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angles in analysis and use of the samples as a reference may alleviate this
problem in future samples received for routine analysis.
     Fiberglass was identified as fiberglass, mineral wool, or glass wool by
all laboratories.  Chrysotile was properly identified by all laboratories.
However, laboratories are experienced in identifying chrysotile, as it is the
most common asbestos fiber found in insulation materials.
4.   PLANS
     The second round of the continuing QA program will start in August 1980,
and will also involve the distribution of samples and reporting of results of
PLM analysis.  The list of commercial laboratories will be updated with perform-
ance ratings and participating laboratories following each round.   Subsequent
rounds are anticipated quarterly.  Future rounds will include samples of
asbestiform and nonasbestiform minerals as well as samples of insulation
materials removed from schools and private buildings across the country.

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          APPENDIX A
INSTRUCTIONS AND REPORTING FORM

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                    ASBESTOS BULK SAMPLE ANALYSIS PROGRAM

Research Triangle Institute                       800-334-8571, ext.  6746

INSTRUCTIONS:

 1.   Verify that this kit contains four bulk material samples containing
     varying amounts of asbestos fibers, nonasbestos fibers, and other
     materials.  Unless the kit is incomplete or contains broken samples,
     fill out and return the enclosed post card.

 2.   If this kit is incomplete or contains broken samples:

          PHONE:    800-334-8571, ext. 6746
          ASK FOR:   Gene Brantly
                    Asbestos Bulk Sample Analysis Program
                    Research Triangle Institute
                    Research Triangle Park, NC  27709

 3.   Perform a complete analysis of each sample by polarized light
     microscopy, with or without dispersion staining.
 4.   Record the requested information on a copy of the Results Reporting
     Form.  Final results of the analyses of all samples should be reported
     on one form.

 5.   Fill in all blocks for each analysis performed, using zeros where
     necessary.  In categories offering numbered choices, write the
     correct number in the appropriate block, followed by a percent
     value or comment.  All reported values should be expressed as
     percentages of the total material in each sample.  Reporting
     forms with incomplete information will not be evaluated.

 6.   Include the laboratory identification number and sample identifi-
     cation numbers on all Results Reporting Forms.  The space labelled
     "Sample Code Assigned by Laboratory" should be used for internal
     quality control programs involving split samples.

     Example        Sample Labels:  2750-01-372
                                    2750-01-149
                                    2750-01-967
                                    2750-01-730

               Laboratory ID# 2750	

               Sample IDJ	01-372   01-149   01-967   01-730

 7.   Complete the reverse side of the Results Reporting Form.

 8.   Retain all samples for reference following receipt of the program
     results and statistical summary.
 9.   Return the Results Reporting Form within 15 working days after
     receipt of the sample kit.

                  Late Returns W111 Not Be Evaluated

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Results Reporting
     Form
ASBESTOS BULK SAMPLE ANALYSIS PROGRAM
                                                   RETURN REPORTING FORM
                                                   WITHIN FIFTEEN WORKING DAYS
                                                   AFTER RECEIVING SAMPLES.
                                                   LATE RETURNS WILL NOT BE EVALUATED.
Laboratory 1.0. #
Sample I.D. #
Sample Coda Assigned by Laboratory
Analytical Method 1. PLM
(enter number) 2. PLM + dispersion staining
3. X-ray diffraction
Gross Sample Appearance 1. Homogeneous, fibrous
(enter number; note 2. Homogeneous, nonfibrous
color) 3. Heterogeneous, fibrous
4. Heterogeneous, nonfibrous
5. Heterogeneous, mixed
Sample Treatment 1. Homogenized
(enter number) 2. Untreated
3. Other, specify
Amount of Material Examined (nig)
Asbestos Present (enter 1. Amoshe
number and percent) 2. Chrysotile
3. Crocidolhe
4. Other, specify
Percent Total Asbestos Present in Sample
Other Fibrous 1. Fiberglass
Materials Present 2. Mineral Wool
(enter number and 3. Cellulose
percent) 4. Other .specify
Nonfbrous Materials Present (description and percent)

































(Continued: Please provide requested information on reverse side of this form.)













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Description of Method of Quantitation
Description of Quality Control Program {e.g., ^slides/sample, ^splits/sat)
Comments
Analyst:
Confirmation by:
Report Reviewed by:
Address Correction Please:
                                                           8

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    APPENDIX B
REFERENCE REPORTS

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                                                   HBatteile
                                                        Columbus Laboratorir*
                                                        505 King Avenue
                                                        Columbus. Ohio 43201
                                                        Telephone (614, 4^-h424
                                                        Telex 24-5454
January 21, 1980
Mr. D. Lentzen
Research Triangle Institute
P.O. Box 12194
Research Triangle Park, North Carolina  27711

Dear Mr. Lentzen:

The X-ray diffraction analyses of your four samples of insulation material
have been completed, with the results given in Table 1.  Only Sample 2 and
Sample 4 produced diffraction patterns.

Sample 1 produced a single broad band between 3.6 X and 2.5 A with maximum
intensity near 2.9 A.  Sample 3 produced a broader band from 4.9 A to 2.5 A
with a broad maximum centered at 3.2 A.
o
  e diffraction patterns were taken using a Phillips vertical goniometer
fitted with a sample spinner and diffracted beam monochromater.   A Phillips
standard focus Cu target X-ray tube was used to produce the incident beam.
The samples were mounted for analysis using double sided adhesive tape.
The sample spinner was used to reduce the effects of sample orientation.

The unused portion of your samples is enclosed.  If you have any questions
regarding these analyses, please call me at (614)  424-5301.

Sincerely,
P. M. Schumacher
Chemist
Physico-chemical Systems

PMS/cln

Enclosure
                                    10
                             50 Years Or Service
                                 1929-1979

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                                     TABLE 1
                X-RAY DIFFRACTION OF ANALYSIS OF INSULATION MATERIAL


Sample                  Compound                    PDF//       Pattern Strength

  #1       No diffraction peaks - see text
  #2       Mg3Si401Q(OH)2 (Talc)                   13-558           Strong
           (Mg, Fe)7Sig022(OH)2 (Anthophyllite)     9-455            Weak
  #3       No diffraction peaks - see text
  #4       Mg3Si205(OH)4 (Clinochrysotile)         27-1276          Strong
                                        11

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            United States Department of the Interior
                          BUREAU OF MINES
                           4900 LASALLE ROAD
                        AVONDALE, MARYLAND 20782

                                          December 28, 1979


Dr. Donald Lentzen
Research Triangle Institute
P.O. Box 12194
Research Triangle Park,  N.C.  27709

Dear Don:

The five reference asbestos samples  submitted on December 10 have been
examined by optical  microscopy and X-ray  diffractometry for mineralogy
and purity.  The results of this  examination  are given below.

   Sample 1:  Mineral  wool  composes  over  98%  of the sample.
              Greater  than  90% of the mineral wool fibers range
              from 5 to  10  ym in  width.   The  minimum width is
              approximately 1  ym.  Less than  5% of the particles
              are glass  globules  or  blebs.

   Sample 2:  This is  a  relatively impure sample with as much
              nonasbestos material as asbestos.  The major
              constituents  in  order of abundance are chlorite,
              talc,  and  anthophyl1ite.  There are minor amounts
              of tremolite  and serpentine also present.  The
              anthophyl1ite is fibrous while  the tremolite ranges
              from blocky to  slightly elongate.  The chlorite
              and talc appear  as  elongated plates, pseudomorphic
              after  the  amphiboles.

   Sample 3:   This sample is composed of  greater than 98% fiber-
              glass.   Over  90% of  the fiberglass is 7 to 20 ym
              in width with a  minimum width of approximately 2 ym.
              Less than  5%  of  the  particles are glass blebs  or
              globules.

   Sample k:   Chrysotile composes greater than 95% of the sample
              with 3%  opaques, less than  1% platy minerals
              (possibly antlgorlte-lizardlte)  and less than  2%
              others.
                                12

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   Sample 5:  This sample Is composed of over 95$ tremolite asbestos
   (R-11)     with 2% to k% carbonate.  The tremolite is predominately
              fibrous with only a small  percentage being blocky.

Best wishes for the New Year.

                                      Sincerely yours,
                                      Will!air J.  Cnmpbcll
                                      Acting Research  Director
                                     13

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      APPENDIX C
REPORTS TO LABORATORIES
          14

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                        ASBESTOS BULK  SAMPLE ANALYSIS PROGRAM
                                     RESULTS  OF  ROUND  1
       Laboratory:

       Laboratory I.D. #:

Sample I.D.*:
Asbestos Present (%)
   Laboratory report
   Reference report

Other Fibrous Material (%)
   Laboratory report
   Reference report
         0
         0
100 mineral wool
 98 mineral wool
75 anthophyllite
53 anthophyllite


       0
       0
     0
     0


95 fiberglass
98 fiberglass
95 chrysotile
95 chrysotile


     0
     0
Summary of Laboratories Reporting;
Mean % (Standard deviation)
  Asbestos present
  Other fibrous material

Distribution of Asbestos Quantitation
       0(0)
     96.1 (5.4)
   53.0(19.3)
    1.4(7.1)
    0(0)
 97.7 (4.0)
 84.5 (17.4)
  1.2(3.1)
 Sample I.D. #:  801
                                    Ji  li 12.S 17.5 22.6  27.S
                                                                                                 " *
                                                         PIHCENTAQS ASBESTOS REPORTED
 Sample I.D. #:  854
                             a  n
                             £  12
                             £  11
                             2  10

                             I
  2.5  7.8
                                                22J 27.8 32J  H.S «Z5 47.8 S2J 87J  «ZJ »7.S

                                                          KMCENTAOC ASIESTOS REPORTED
                                                                                   77.6  K.5  «7.» 92.5 97J
                                                   15

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       APPENDIX D
COMMERCIAL LABORATORIES
          16

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 American Can Company
 Safety & Industrial Hygiene Laboratory
 U.S. Highway 22
 Union, New Jersey  07083

 American Microscopy Laboratory
 D.  3410 12th Avenue E.
 Tuscaloosa, Alabama  35405

 Analytical Center, Inc.
 P.  0.  Box 15635
 Houston, Texas  77020

 Boeing Technology Services
 9R-25
 P.  0.  Box 3707
 Seattle, Washington  98124

 Brewer Analytical Laboratories
 311 Pacific Street
 Honolulu,  Hawaii  96810

 C.E.D.,  Inc.
 Environmental Microscopy International
 135 West Cutting Blvd.
 Richmond,  California  94804

 Casalina Associates, Inc.
 47-345 Mahakea Road
 Kaneohe,  Hawaii  96744
 Certified  Testing  Laboratories,
 2905 East  Century  Boulevard
 South  Gate,  California   90280
Inc.
 Clayton Environmental  Consultants,  Inc.
 25711  Southfield Road
 Southfield, Michigan   48075

 Colorado School of Mines
 Research Institute
 P. 0.  Box  112
 Golden, Colorado  80401
    Goldblatt
407 N. Butrick St.
Waukegan, Illinois  60085

Continental Technical Services
Environmental Health Division
9742 Skillman
Dallas, Texas  75243
 Department  of  Chemistry
 New Jersey  Institute  of Technology
 323 High Street
 Newark,  New Jersey  07102

 Department  of  Geological Sciences
 SUNY,  New Paltz
 New Paltz,  New York   12562

 Department  of  Geology
 Illinois  State University
 Normal,  Illinois  61761

 Eastern  Analytical Laboratories
 One "A"  Street
 Burlington,  Massachusetts  01803

 EMS Laboratories
 12563  Crenshaw Boulevard
 Hawthorne,  California  90250

 EMV Associates, Inc.
 Microanalysis  Laboratory
 15825  Shady Grove Road
 Rockville,  Maryland   20850

 Environment/One Corporation
 2773 Balltown  Road
 Schenectady, New York  12301

 Environmental  Consulting & Testing Services
 P.  0.  Box 3521
 Cherry Hill, New Jersey  08034

 Environmental  Health Services, Inc.
 5206 Lindbergh Blvd.
 W.  Carollton,  Ohio  45449

 Erie Testing Laboratories
 2401 W. 26th Street
Erie,  Pennsylvania  16506

Erlin, Hime Associates
 811 Skokie Boulevard
Northbrook,  Illinois  60062

GCA Corporation
Techology Division
Burlington Road
Bedford,  Massachusetts  01730
                                          17

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  Geoscience Consultants,  Inc.
  P.  0.  Box 341366
  Coral  Gables,  Florida  33134

  Hager  Laboratories
  12000  E.  47th  Avenue
  Denver, Colorado 80239

  Health Science Associates
  Suite  B/C
  10941  Bloomfield Street
  Los Alamitos,  California  90720

  Herron Testing Laboratories
  5405 Schaaf Road
  Cleveland, Ohio  44131

  IIT Research Institute
  10 West 35th Street
 Chicago,  Illinois  60616

 Industrial Analytical Laboratory
 1523 Kalakaua Avenue
 Suite 101
 Honolulu,  Hawaii  96826

 Industrial Hygienics,  Inc.
 755  New York Avenue
 Huntington, New York  11743

 Industrial Testing Laboratories,  Inc.
 2350 Seventh Blvd.
 St.  Louis,  Missouri   63104

 Inter-City Testing & Consulting Corporation
 P. 0. Drawer "0"
 609  Middle  Neck Road
 Great Neck,  New York  11023

 Interscience  Research
 2614 Wyoming  Avenue
 Norfolk, Virginia 23513

 Jesse H. Bidanset &  Associates, Inc.
 P- 0. Drawer  "0"
 609 Middle Neck Road
 Great Neck, New York   11023

Law Engineering Testing Company
3301 Winton Road
Raleigh, North  Carolina  27619
 LTE  Corporation
 Environmental Analysis Lab Division
 2030 Wright Avenue
 Richmond, California  94804

 Maryland Mineral Analysis Laboratory
 Department of Geology
 University of Maryland
 College Park, Maryland  20740

 MJH Associates
 Mineralogical Consultants
 13345 Foliage Avenue
 Apple Valley, Minnesota  55124

 Northrop Services,  Inc.
 P. 0. Box 12313
 Research Triangle Park,  North Carolina   27709

 PEDCo Environmental,  Inc.
 11499 Chester Road
 Cincinnati,  Ohio   45246

 Princeton Testing Laboratory
 P.  0.  Box 3108
 Princeton, New Jersey 08540

 R.  J. Kuryvial &  Associates
 Mineralogy/Microscopy Consultants
 12185 W.  29th Place
 Lakewood,  Colorado  80215

 Southwestern  Laboratories
 P.  0. Box  10687
 Dallas, Texas  75207

 St. Paul Fire & Marine
 Environmental Services Analytical Laboratory
 494 Metro Square Building
 7th and Robert Streets
 St. Paul, Minnesota  55101

 Sunbelt Associates, Inc.
 6961 Mayo Road
 New Orleans,  Louisiana  70126

 Thomas A. Kubic & Associates
 8 Pine Hill Court
Northport, New York  11768
                                         18

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 Tri-State Laboratories,  Inc.
 54  Westchester  Drive
 Austintown,  Ohio   44515

 Truesdail Laboratories,  Inc.
 4101 N. Figueroa  Street
 Los Angeles,  California   90065

 United States Testing Company, Inc.
 1415 Park Avenue
 Hoboken,  New Jersey  07030

 Utah Biomedical Test Laboratory
 520 Wakara Way
 Salt Lake  City, Utah  84108

 Walter McCrone Associates, Inc.
 2820 S. Michigan Avenue
 Chicago,  Illinois  60616

Wausau Insurance Companies
Environmental Health Laboratory
2000 Westwood Dr.
Wausau, Wisconsin  54401
                                         19

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    APPENDIX E
LABORATORY DATA
      20

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                     CODING KEY FOR ABSAP PR INT-OUT
 CO      = commercial
 NC      = non-commercial
 LAB     = laboratory  ID #
 SAM     - sample lot  (1,2,3,4)
 A1P + A2P = ALLP
 01P + 02P = OSUMP
 Method (METH)
 0       = no information
 1       = PLM
 2       * PLM  with dispersion staining
 3       = PLM,  confirmed by XRD
 Treatment  (TRT)
 1        =  homogenized
 2        =  untreated
 3        =  acid wash
 4        =•  ashed
 5        =  mortar  and pestle
 6        =  particle picking
 7        =  rolled  and quartered
Asbestos present  (Al, A2)
1       = amosite
2       = chrysotile
3       = crocidolite
4       = anthophyllite
5       = actinolite
6       = tremolite
7       = "amphibole"
8       a "serpentine"
Other fibrous materials (01. 02)
1       = fiberglass
2       = mineral wool
3       = vegetable fiber (cotton, cellulose, paper)
4       = synthetic fiber (nylon, rayon, etc.)
5       = glass wool
6       » fibrous talc
7       = wollastonite
                                    21

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ro
ro
LABORATORY VALUES
DBS L*B NCTH TRT At
t
2
3
4
5
6
7
" 8
Q
10
It
12
13
14
IS
16
17 2
Z o
2 0
1 0
2 0
1 0
Z 1
Z i
6 0
6 0
6 0
5 2
2 o
Z 0
t o
1 «
1 4
t 6
18 220
19 226
2o 220
21 220
22 I
23 |
2 0
2 6
24 220
25 1
Z 2
26 220
27 I
2 0
28 220
29 I
3o 2
3| 2
32 2
31 1
34 2
35 3
36 ]
37 1
38 0
39 |
40 1
11 2
"2 1
43 2
44 2
45 3
46 2
47 2
48 2
49 t
SO 2
C • *t
51 2
52 I
2 6
t 6
2 6
1 0
3 0
2 6
2 6
2 0
Z 0
0 0
2 0
I 0
2 0
2 0
7 o
1 o
t 0
2 o
1 0
1 0
3 0
2 o
2 6
1 0
AlP
0
0
0
0
0
75.0
80.0
0
0
0
T
0
0
0
0
0
0
0
0
0
0
0
0
0
T
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
^«Wt^»"
A2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
AZP ALLP
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
75.0
80.0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
01
2
2
t
2
2
0
0
Z
2
2
1
2
2
1
1
2
2
2
2
2
2
2
2
2
2
S
2
2
2
2
1
2
1
I
2
2
2
2
2
2
2
2
2
2
Z
Z
Z
2
2
2
I
2
01P
100.0
85.0
«s.o
100,0
97,0
0.0
0.0
85,0
80,0
90.0
87,5
95.0
50,0
95,0
95.0
100.0
100,0
95.0
100,0
100,0
30,0
95,0
95,0
95,0
98.0
96,5
100,0
95,0
99,0
98,0
75,0
99,0
95,0
100,0
98,0
100,0
99,0
100,0
100,0
100,0
90,0
94,0
95,0
95,0
98,0
70,0
100,0
98,0
100,0
100,0
97,5
'a. s
02
0
3
2
0
0
o
o
3
3
3
0
0
1
0
3
0
0
0
3
0
1
0
0






0
2
0
1
o.
3
0
0
0
0
0
0
3
0
0
3
0
0
3
0
3
0
0
02P
0,0
5,0
45,0
0,0
o.o
0.0
0.0
7,0

OSUMP
100,0
90
90
.0
.0
100,0-
97
0
0
92
8S
.0-
.0
.0 >
.0-
.0 •
t'l 9215
0,0
0.0
40,0
6.0
o.o
0.0
0.0
o.o
0,0
o.o
70.0
o.o
06
00
l.o
o.o
00
i.o
o.o
o.o
24.0
o.o

o.'o
2,0
o.o
o.o
0.0
00
0.0
o.o
1,0
0,0
0.0
*••
0,0
6.0
0,0
6.'o
0.0
O.o
87,5,
95.0.
90,0
95,0
95,0
100.0
100,0
95,0
100,0
100,0
100.0
95.0
95,0
95,0
99,0
96,5
100,0
96,
99,
It.
99,
99,
0
0
0
0
0
100,0
too,
100.
100.
99,
100.
too,
too.
0
0
0
0
0
0
0
90,0

n
9s|o
95.0
98,0
70,0
100,0
98,0
100,0
100,0
97, S

)

-------
ro
CO
003
53
So
55
56
57
58
54
60
61
62
63
6a
65
66
67
68
69
70
71
72
73
74
75
76
77
70
79
80
81
82
«3
8a
8S
86
87
88
89
90
91
92
93
9a
95
96
97
98
99
100
I0|
102
103
104
LAB HETH
2






2
2
2






2
2
2
2
3
3
1
2
1
2
1
2
I
2
2
J
1
3






2
3
Z
2
3
2
2
2
1
2
2
2
TRT
2
2













I
1
2
t
2
i
1
2
2
2
2
2
2
2
1
2
1
3
t
2
2
2
0
t
1
2
2
7
1
1
2
1
1
3
2
2
1
Al
6
7
1
4
1
1
3
a
4
4
1
4
1
4
4
1
1
4
4
4
4
4
4
4
4
4
4
6
4
6
f
4
1
4
4
8
1
1
J
4
4
4
4
3
4
6
4
4
0
6
4
4
A1P
60.0
SO.O
40.0
75.0
22.5
80.0
90.0
50.0
50.0
40.0
45.0
93,0
60.0
SO.O
60.0
40.0
65.0
60.0
SO.O
62.5
95.0
62.5
40.0
60.0
50.0
87.5
15.0
70.0
50.0
50.0
40.0
SO.O
90.0
50.0
23.0
SO.O
SO.O
20.0
30.0
74.0
70.0
25.0
45.0
40.0
20.0
30.0
SO.O
60.0
40.0
49.0
40.0
65.0
_Nlc
A2
0
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
t
2
0
LABORATORY VALUES

           SAM«2 -

           A?P

           O.'O
           O.'O
          15.0
           O.'O
           0,0
           0.0
           O.'O
           0,0

           o>
           o.o
           0,0
           0,0
           0,0
           00
           ojo
           ojo
           0.0
           0.0


           0^0
           0,0

           2*3
           0>
           0,0
           0,0
           •if
           0,0
           0,0
           o.o
           o.o
           0,0
           o;o
           0,0
           0,0

           0>
           0,0
           o.o
           o.o
           0,0

           o'J
           olo
           o.o
           0,0
           o.o
           1.0
           2:0
           o.o
ALLP

60,0
so.o
55.0
79.0
«.s
00.0
90,0
so.o
50.0
40.0
45.0
93.0
60.0
so.o
60.0
40.0
65.0
60.0
so.o
62.5
'5.0
62.5
40.0
60.0
52.5
87.5
15,0
70.0
SO.O
SO.O
40,0
so.o
90.0
so.o
23.0
SO.O
so.o
20.0
10.0
70.0
70.0
25.0
45.0
40.0
20.0
30.0
SO.O
60.0
40,0
50.0
42.0
6S.O
01

0
0
I
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
9
0
0
t
OIP

  0
  0
 10
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  1
  0
  0
  0
  0
  0
  0
  0
  0
  0
 10
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
 50
  0
  0
  0
  0
  0
  0
  0
  0
  3
  0
02

0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
3
0
0
02P

 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
03UMP

  0
  0
 10
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  0
  I
  0
  0
  0
  0
  0
  0
  0
  0
  0
 10
  0
  0
  0
  0
  0
  0
  0
  0
                                                                                                                     5

-------
ro
-P.
LABORATORY VALUES
OBa
105
106
107
108
109
Ito
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
13o

132
133
134
135
136
137
138
139
140

142
113
144
145
1
-------
                                                                LABORATORY VALUES
ro
en
I)BS
157
156
159
160
161
162
163
160
1*5
166
167
166
169
I/O
171
172
173
170
175
176
177
176
179
160
161
182
163
iea
165
|66
187
|66
169
ivo
191
192
193
.|90
195
196
197
198
J99
200
201
202
203
290
205
206
20T
208
LAB METH
2






2
2
2






2
2
2
2
2
1
1
2
1
2
1
2
1
2
2
2
1
3
3
1
1
0
1
1
2
I
2
2
3
2
2
2
1
2
i
2
TRT
2
2
1
1
t
2
2
6
6
6
5
t
2
1
t
1
1
2
1
2
2
1
2
2
2
2
2
2
2
1
2
1
3
1
2
2
2
0
1
1
2
2
7
I
t
2
1
1
3
2
Z
1
At
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
i
2
2
2
2
2
0
2
2
2
*|P
95.0
90.0
65.0
95.0
96.0
90.0
95.0
75.0
90.0
70.0
95.0
93.0
60.0
95.0
96.0
60.0
loo.o
95.0
96.0
99.5
98.0
80.0
98.0
95.0
97.5
82.5
10.0
95.0
92.5
85.0
95.0
99.0
97.0
30.0
80.0
90.0
95.0
01.0
65.0
65.0
92.5
90.0
90.0
75.0
100.0
65.0
95.0
97.0
60.0
85.0
50.0
90.0
<--.N«.3
*2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
A2P

 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 ALLP

 95.0
 90.0
 65.0
 95.0
 9S.O
 90.0
 95.0
 75.0
 90.0
 70.0
 95.0
 93.0
 60.0
 95.0
 96.0
 60.0
100.0
 95.0
 96.0
 99.5
 9S.O
 80. 0
 98.0
 95.0
 97.5
 82.5
 10.0
 95.0
 92.5
 65.0
 95.0
 99.0
 97.0
 JO.O
 60.0
 90.0
 95.0
 41.0
 65,0
 65.0
 93.5
 90.0
 90.0
 75.0
100.0
 65.0
 95,0
 97.0
 60.0
 85.0
 50,0
 9a.O
01

0
0
t
0
0
0
0
i
1
1
0
0
0
0
0
0
0
0
0
0
I
0
0
0
0
0
0
0
0
3
t
0
0
1
0
0
0
0
0
0
0
0
0
2
0
0
0
0
0
0
2
0
 01P

 o.o
 0.0
is.o
 o.o
 0.0
 o.o
 o.o
 5.0
 2.5
 2.5
 o.o
 o.o
 o.o
 o.o
 o.o
 o.o
 o.o
 0.0
 o.o
 o.o
 2.0
 o.o
 o.o
 o.o
 o.o
 0.0
 o.o
 o.o
 0.0
 7.5
 ».o
 o.o
 o.o
10.0
 o.o
 0.0
 o.o
 o.o
 o.o
 0.0
 0.0
 0.0
 0.0
 5.0
 o.o
 o.o
 0.0
 o.o
 0.0
 0,0
10.0
 o.o
02

0
3
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
4
0
0
0
0
0
0
3
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Off

 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 1
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
OSUHP

  0,0
  0,0
 15,0
  0,0
  0,0
  0,0
  0,0
  5,0
  2,5
  2.5
  0,0
  0,0
  0,0
  0,0
  0,0
  0.0
  0,0
  0.0
  0,0
  0,0
  2.0
  0,0
  0,0
  0,0
  0.0
  0,0
  0,0
  0,0
  0,0
  7,5
  2,0
  0.0
  0.0
 10.0
  0,0
  0,0
  0,0
  0,0
  0,0
  0,0
  0.0
  0,0
  0,0
  5,0
  0.0
  0.0
  0.0
  0.0
  0,0
  0,0
 10,0
  0,0

-------
083

 t
 Z
 J
 4
C_NC

 CO
 CO
 CO
 CO
SAM

 1
 2
 J
 4
 0.00i)o
84.5«8i
        DESCRIPTIVE SUTISTICS

M_AUP    M-U3UMP      S_*LLP     3_OSl)MP

O.OOUO    96.1100      o.OOOQ
           l.«^ll    I
          97,6827
            5.1670
            r,1465
 o.OOOO     1.9668
17.4410     1,0647
N_*LIP

  SO
  S2
  SZ
  52
                                                                                                              N..OSUMP

                                                                                                                SO
                                                                                                                52
                                                                                                                52
                                                                                                                S2
ro

-------
TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1.
4.
7.
9.
REPORT NO.
EPA 560/13-80-23
TITLE AND SUBTITLE
Asbestos-Containing Mate
Bulk Sample Analysis Qua
AUTHOR(S)
E.P. Brantly, Jr. and D.
PERFORMING ORG "VNIZATION NAME Af>
Research Triangle Instit
P.O. Box 12194
Research Triangle Park,
12. SPONSORING AGENCY NAME AND ADC
Survey and Analysis Divi
Office of Pesticides anc
.U.S. Environmental Prote
Washington, DC 20460
2. 3. RECIPIENT'S ACCESSION>NO.
5. REPORT DATE
rials in School Buildinas: August 1980 preparation
lity Assurance Program 6. PERFORMING ORGANIZATION CODE
date

8. PERFORMING ORGANIZATION REPORT NO.
E. Lentzen
JD ADDRESS 10. PROGRAM ELEMENT NO.
,ute
11. CONTRACT/GRANT NO.
NC 27709 Contract No. 68-01-5848

-
JRESS 13. TYPE OF REPORT AND PERIOD COVERED
sion Final
1 Toxic Substances 14- SPONSORING AGENCY CODE
jction Agency

15. SUPPLEMENTARY NOTES
16. ABSTRACT
EPA has initiated a quality assurance (QA) program for laboratories
claiming capability in the polarized light microscope (PLM) analysis of
bulk samples for asbestos. Commercial and non-commercial laboratories
participating in the program received samples of four fibrous materials:
chrysotile, anthophyllite, fiberglass, and mineral wool. Laboratories
had difficulty identifying anthophyllite and two false positives were
reported for the mineral wool sample. All laboratories properly identi-
fied chrysotile. A performance rating based on proper identification
of positive(asbestos) and negative(non-asbestos) samples was scored for
and reported to the commercial laboratories. Reference reports were
sent to all participating laboratories. Continuation of the program
with future sample sets is anticipated.
17
a.

18

DESCRIPTORS
Asbestos
Serpentine
Amphi boles
, DISTRIBUTION STATEMENT
Release to public
KEY WORDS AND DOCUMENT ANALYSIS

b.lDENTIFIERS/OPEN ENDED TERMS C. COSATI Field/Group
Bulk Sample Analysis
19. SECURITY CLASS (This Report) 21. NO. OF PAGES
Unclassified 27
20. SECURITY CLASS (This page) 22. PRICE
Unclassified



EPA Form 2220-1 (9-73)
                                                           27

-------