APPLICABILITY OF  EXISTING METHODS
                      FOR THE DETERMINATION
              OF THE BIOCHEMICAL  OXYGEN DEMAND  (BOD)
                   OF INCINERATOR QUENCH WATER
             A Division of Research and Development
                 Open-File Report  (RS-03-68-18)
U.S.  DEPARTMENT OF  HEALTH,  EDUCATION, AND WELFARE
                    Public Health Service

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       APPLICABILITY OF EXISTING METHODS

             FOR THE DETERMINATION

     OF THE BIOCHEMICAL OXYGEN DEMAND (BOD)

          OF INCINERATOR QUENCH WATER
    A Division of Research and Development
        Open-File Report (RS-03-68-18)
                   written by
       Donald L.  Wilson, Research Chemist
U.S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE
    Public   Health   Service
          Environmental Health Service
        Bureau of Solid Waste Management
                      1970

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Summary of BOD-Quench Water Study
Donald L. Wilson
An investigation was undertaken to determine if commonly used procedures
for BOD determination in water pollution control were applicable to
incinerator quench waters.
Personnel in water pollution control are employing DO meters, calibrated
using the Alsterberg (Azide) Modification of the Winkler Method. The DO
meter commonly used is produced by the Weston and Stack Company.
Although the Weston and Stack (DO) Analyzer was found applicable in
determining BOD of incinerator quench water, quick qualitative tests
were established which would determine if the Azide Modified Winkler
Method is also applicable. These qualitative test are for those who do
not have a DO meter and do not wish to buy one unless their particular
samples require one. It is thought that only in rare instances could
the Winkler (Azide) Method be employed to determine BOD of incinerator
quench water.
Since the DO Analyzer may be used in the field where comparison of the
Analyzer to the Modified Winkler Method may be difficult, a procedure for
an easy field check on the analyzer’s performance was developed. In this
field-operation-check method, each analyst must determine the ppm
value for his particular instrument while the instrument is functioning
correctly. This field-operation-check method does not and was not meant
lii

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to replace the actual calibration procedure involving the upper point,
with sample at saturated oxygen concentration and the lower point, with
sample at zero oxygen concentration.
Quench water samples were found to have BOD values (5-day basis) ranging
about from 100 to 300 ppm. The samples normally required a dilution
factor of L O or 2.5 percent dilution; however, whenever possible the
analyst should employ more sample in order to reduce the possible error
in the BOD result. No modifications of existing methods were found
necessary, however, the seeding technique or the immediate DO analysis
are not recommended. Seeding requires some knowledge of the organisms
present in the samples. Therefore, finding the appropriate seeding
material for quench water would be very difficult. Immediate DO demand
is sometimes employed to separate biological from chemical demand. But,
the immediate DO demand test is difficult to perform at an incinerator
site and the chemical effects upon the immediate test cannot be minimized
by the aeration step which is performed on all samples in the 5-day test.
Much of this investigation dealt with concepts already verified, there-
fore, statistically sufficient data to prove each statement was not
required in this study.
iv

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TABLE OF CONTENTS
Page
Abstract 1
Introduction 2
Approach 3
Results
Conclusions 13
References 11+
Appendix
I. Tables and Figures 15
II. Mechanism of Alsterberg (Azide) 28
Modification of the Winkler Method
III. Mechanism of Weston and Stack (DO) Analyzer 29
IV. Qualitative Tests for Impurities 30
V. Cost of BOD Methods 35
V

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REPORT ON THE APPLICABILITY OF EXISTING METHODS FOR THE DETERNINATION
OF THE BIOCHEMICAL OXYGEN DEMAND (BOD) OF INCINERATOR QUENCH WATER
Donald L. Wilson*
Abstract
For many years the Alsterberg (Azide) Modification of the Winkler
(Dissolved Oxygen) Method has been used for the determination of the
Biochemical Oxygen Demand (BOD) of water samples from lakes, rivers,
streams, sewage, and industrial waste waters. When this procedure was
employed, to characterize incinerator quench water ’ ’ many interferences
were encountered. Many of the interfering substances found in quench
water are also in those water samples mentioned above but in lower
concentrations. This report describes the analytical problems created
by interferences and demonstrates how they may be overcome using a
dissolved oxygen analyzer. Since such an instrument may not be available
in every laboratory, this summary also discusses some qualitative tests
for interferences and under what circumstances the Alsterberg (Azide)
Modification of the Winkler Method may be employed.
Using 5-day BOD basis, incinerator quench water samples normally had BOD
values ranging about from 100 to 300 ppm. A dilution factor of ‘ O or 2.5
percent dilution (25 ml of sample diluted to one liter) was usually
Research Chemist, Bureau of Solid Waste Management, Public Health Service,
U.S. Department of Health, Education, and Welfare.
The BOD test is essentially a bio-assay of oxygen loss during standardized
incubation.
* In this report quench water refers to that water which has been employed
by the incinerator staff to quench the residue just after it emerges from
the furnace and before it is transported to its disposal site.

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—2—
employed with these samples. With the above restrictions, the read-
ability of the apparatus will not afford BOD values below 23.5 ppm or
5 4.l PPm) DO Analyzer or Modified Winkler Method, respectively.
Introduction
During the quenching of incinerator residues, the water becomes contam-
inated with large amounts of living organisms. To determine the biological
metabolism in this polluted water, the analyst performs a biochemical
oxygen demand test which measures the amount of dissolved oxygen required
for oxidation of the organic matter by microbial action in the presence
of oxygen.
The oxygen demand of incinerator quench water (or similarly contaminated
water) is exerted by three classes of materials: (a) carbonaceous
organic material usuable as a source of food by aerobic organisms;
(b) oxidizable nitrogen derived from nitrite, ammonia, and organic
nitrogen compounds which serve as food for specific bacteria (e.g., Nitro-
somonas and Nitrobacter Species); and (c) certain chemical reducing
compounds (e.g., ferrous iron, sulfite, and sulfide) which will react
with molecularly dissolved oxygen. Since the oxidation of nitrogeneous
materials may proceed at a variable rate, even delayed for several days
unless suitable micro-biota are available, the nitrification process is
usually inhibited. In this study, nitrification was inhibited by the
recommended pH adjustment technique, that is, samples are acidified to
pH 2 to 3 and subsequently neutralized (pH 6.5 to 8.3). The inhibition

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—3—
of the nitrification process restricted the BOD test to the carbonaceous
demand and presumedly allowed for a better correlation between BOD values
of quench water samples.
Complete stabilization of a given sample, at 20 C, may require a period
of incubation too long for practical purposes. For this reason, the
5-day period has been accepted as the standard time for BOD analysis.
Conversion of data from this incubation period to another can only be
made if oxidation curves (BOD at various incubation periods) have been
prepared for the type of samples being investigated.
Approach
Since the DO analysis is an established procedure in water pollution
control, some of the scientists familiar with this analysis were con-
sulted. Their recommendations and a literature review suggested that
there were only two practical methods, the Alsterberg (Azide) Modifica-
tion of the Winkler Method” 2 and a dissolved oxygen analyzer method.
The type of dissolved oxygen analyzer employed in this method develop-
ment was the recommended Weston and Stack, Model 300.
The Alsterberg (Azide) Modification of the Winkler Method and the Weston
and Stack (DO) Analyzer were employed in determining the BOD of quench
waters collected at a local incinerator. By the application of these
methods, the analytical difficulties and sample dilutions were revealed.
Product (or manufacturer) identification in this report does not imply
endorsement by the United States Public Health Service.

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—
Studies were performed to determine if those substances which interfered
with the Modified Winkler Method also affected the response of the DO
Analyzer. Qualitative tests were developed to detect the presence of
these interfering substances. Some of these tests are not element or
compound specific but do detect any substance that will behave as the
known interfering substances. These tests will enable the analyst to
decide if the Modified Winkler Method is applicable to a particular
sample.
Since the DO Analyzer may be used in the field, the analyst needs some
field method for determining if the analyzer is functioning correctly.
Studies were performed to establish a simple field test which (1) would
not require as much apparatus as the Winkler (Azide) Method, (2) would
not involve gas cylinders or heavy equipment, and (3) would relate more
than just one point (as the concentration value for dissolved oxygen
with complete saturation at a particular temperature). The field test
is not to supersede the regular calibration procedure but to reaffirm
that the instrument’s linear scale is functioning properly. The approach
in this investigation was to find either a medium that had an oxygen
solubility value markedly different from that of water or a substance
that would react with a definite amount of oxygen in water.
Results
The Alsterberg (Azide) Modification of the Winkler Method was applied to
quench water samples collected at the Red Bank Incinerator, Cincinnati,
Ohio. All samples were collected at one time, in sterile (no matter

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—5.-
present that contributed to BOD value) plastic containers, and trans-
ported immediately to the laboratory where they were promptly analyzed
without any pH adjustments to inhibit the nitrification process nor
addition of potassium fluoride solution to complex any ferric iron that
might be present. The time that elapsed between collection and initia-
tion of analyses was three hours (If hours is the suggested maximum
time without refrigerating sample).
The BOD values of samples, collected from pools in the residue disposal
area, range from 107 ppm to 292 ppm (Table 1). Analyses of grab samples
of quench water draining from a truck, employed to haul residue to
the disposal area, always yielded erratic results (Table 1).
A dilution procedure using 50 ml of sample diluted to one liter although
producing less possible error in BOD results, in our study was unsat-
isfactory because sometimes less than one ppm of dissolved oxygen was
present in the sample after the incubation period (samples 1 and 2 of
Table 1). A dilution factor of 14Q or 2.5 percent proved satisfactory
for the general BOD values (100 ppm to 300 ppm) of quench water. Since
duplicate determination did not always agree (one example being Sample
Lt, Table 1) and the instability of samples usually prevented repeating
the (5-day) BOD analysis at a later date (Table 2), triplicate final DO
determinations will afford the analyst more assurance in obtaining
reasonable duplicate results (usually only duplicate results are reported).
Also the BOD test should be initiated within L hours after sample
collection, however, it is reported that if samples are stored, immediate-
ly after collection, at 50 then the initiation of the BOD test may be

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-6-
delayed up to 2L hours. In order to obtain triplicate BOD determina-
tions (two initial DO and three final DO) with the Modified Winkler
Method, two liters of solution (50 ml of sample) is required instead
of the normal one liter.
Qualitative tests (Appendix) were employed to analyze these quench
water samples for impurities (oxidizing and reducing substances) known
to affect the Winkler Method. These tests, when applied to the before
mentioned samples, revealed that the samples contained non specific
substances that would produce interferences similar to those caused by
nitrites, ferric compounds, and sulfites.
Although a pH meter allows more exacting control, ordinary pH paper was
believed sufficient to determine the pH of all the samples collected at
the Red Bank Incinerator site. The results of this analysis revealed
that the quench water samples are basic (pH of 10 to 11) and definitely
need a pH adjustment to inhibit the Nitrification process before the
BOD analysis is initiated. Besides all samples should be neutralized,
if not already, to about pH 7 (pH of 6.5 to 8.3) because of the reported
affects upon the incubation period and the Winkler Method of Analysis.
Additional quench water samples were collected from the Red Bank Incin-
erator for the purpose of (1) studying the effects of sample preparation
or alteration upon the BOD results; and (2) comparing the Modified
Winkler Method to the DO Analyzer method. All these samples were acquired
at one time, in plastic containers (free of substances which have a BOD
value). A BOD analysis of a 50 ml to 2 liter-dilution of each sample was

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—7-
initiated, with and without Nitrification inhibition, four hours after
collection. Considering the DO Analyzer to disclose the correct BOD
values, the values for quench water (Table 3) were within the 107 ppm
to 292 ppm range previously found. The BOD results showed poor precision,
between duplicate analyses and duplicate samples, with both the Modified
Winkler Method and the DO Analyzer when quench water samples were collec-
ted from water draining off a truck loaded with residue (Tables 1 and
3).
The qualitative impurities tests performed on these samples revealed the
presence of substances which would affect the Modified Winkler Method.
This effect is similar to one produced by samples containing nitrites,
ferrous atd ferric irons, and sulfates. One sample, which was collected
down hill from piles of salt (stored there for icy streets), contained
an excessive amount of chlorides. (Although these tests were employed
only qualitatively, excess concentration of interferences was easily
detected). A later study revealed that, although chlorides do not
normally interfer in the BOD test, when an unusually high concentration
of chlorides is present in a sample, gas is released during the Modified
Winkler Method and the BOD results are affected.
The results of a pH test on these samples again revealed the quench water
samples to be alkaline (pH of 10 or 11). Since our tests have indicated
(Table L i) and authorities on the BOD test have reported, that pH values
other than 6.5 to 8.3 and the Nitrification process can affect the BOD
analysis thereby producing unreliable BOD values, all quench water should

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—8-
be acidified to inhibit nitrification and then neutralized to a pH of
6.5 to 8.3 before the BOD analyses are performed.
After analyzing a number of quench water samples, the pooled standard
deviation of the observations by each method (Modified Winkler and
Weston and Stack Analyzer) was evaluated. All observations were employed
in these calculations except those (1) obtained on samples collected
from dump truck drainage and (2) in which less than one ml of titrant
was employed in the Winkler titration. In the BOD study of quench water
samples from the Red Bank Incinerator, DO data obtained by using the
Weston and Stack Analyzer was more reproducible than data obtainable
with the Modified Winkler Method (Table 5 and 6).
In order to ascertain that Weston and Stack (or Yellow Springs) Analyzer
can better determine DO of quench water than the Modified Winkler Method,
impurities, commonly found in contaminated waters and reported to effect
the Modified Winkler Method were investigated as to their degree of influ-
ence upon each method. One of these impurities is nitrite which when
added to water samples (standard BOD dilution medium saturated with
oxygen) produced no change in the Analyzers’ readings; but, with the
Modified Winkler Method produced (above an initial concentration of ‘+-5
ppm nitrite) an 82 percent increase in DO concentration per ppm nitrite
in the 300 ml BOD bottle (Figure 1). Although the Alsterberg Modifica-
tion attempts to compensate for the presence of commonly found concentra-
tions of nitrites, it clearly cannot be employed in the presence of
unusually large nitrite concentrations (greater than 14-5 ppm nitrite in

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-9—
BOD bottle). (Such high nitrite concentrations could possible exist
in water discharged from industrial complex; however, quench water from
incinerators is not expected to achieve such high nitrite concentrations).
Nitrite Effect: 2HN0 2 1- 2H1 —p 12 + 2H 2 0 + N 2 0 2 —i 2N 2 0 2 + 2H 2 0 ÷ 02 4HN 2
Evaluation of the effect of sulfite solution upon the DO determination
revealed almost no change (only about 0.4 percent per ppm sulfite
because of oxygen depletion) in the DO Analyzers’ readings while the
Modified Winkler Method showed a decrease, in dissolved oxygen analysis,
of 5.5 percent per ppm sulfite in 300 ml BOD bottle (Figure 2).
The sulfite effect upon the Modified Winkler Method is linear and, if
the sulfite concentration of a sample is known, a correction factor
could be applied to the BOD value; however, the true sulfite effect
may not be known because in highly alkaline conditions, as in the Winkler
Method, polythionates and similar organic sulfur compounds break down to
form sulfites and thiosulfates, 5 thus increasing the total sulfite effect.
Sulfite Effect: Mn(0H 2 ) + 3H 2 S0 3 1- 202 — Mn(S0 4 ) 2 + H 2 S0 4 + 3H 2 0
When the addition of iron was investigated, the DO Analyzers were not
affected; but the Modified Winkler Method showed a decrease in dissolved
oxygen analysis. This decrease was about 0.7 percent per ppm ferrous
iron in the 300 ml BOD bottle (Figure 2). Ferric iron had no effect
upon the method until at least 50 ppm of ferric iron was placed in the
BOD bottle. Then the Modified Winkler Method showed an increase of 2.3
percent in the DO value. After 100 ppm (total) of ferric iron was added
to the BOD bottle, a DO value increase of 3.1 percent was observed.

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- 10 -
As with the sulfites, analyzing a sample for the amount of ferric and
ferrous iron present will now allow a correction factor to be applied to
the BOD value. The effect of each iron salt varies because several
possible reactions are involved and each of these reactions does not go
to completion. 6
Ferrous Effects: Fe + 2 ÷ —i Fe +
2Fe 2 +I 2 — 2I +2Fe 3
Ferric Effects: FeC1 3 + 2NaI + Na 2 S 1 0 6 12 + 2Na 2 S 2 0 3 + FeC1 2 ÷ C1
+3 -
2Fe i-21 —41 2 +2Fe
Since the Modified Winkler Method is still employed to calibrate the DO
Analyzers, some aspects of the method were investigated. Stability of the
sodium thiosulfate solution and variation in its standardization procedure
could ultimately affect the BOD results. Tests showed that a solution of
sodium thiosulfate (chloroform added as a preservative) is stable for, at
least, lL days and may be standardized using either the potassium dichromate
method or the biniodate method (Table 7).
The Weston and Stack Analyzer may be used in the field where comparison
of the Analyzer (recently calibrated) to the Modified Winkler Method may
be difficult. The development of an easy field calibration or check on
the Analyzer’s performance involved investigating various materials (Table 8).
None of the mediums evaluated had an oxygen solubility which vary measur-
ably from water and thus satisfactory for a field calibration check.
Efforts were, therefore, directed to finding a substance which would
react with a definite amount of dissolved oxygen and provide a simple field

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- 11 -
operation check of the Weston and Stack Analyzer.
Several substances tried produced a detectable but non-reproducible
change in the oxygen concentration. Only those substances which showed
a good measureable change in the dissolved oxygen value are discussed
herein. One such substance was sodium sulfite (regularly used to zero
DO meters). However, the reaction of sodium sulfite with the dissolved
oxygen does not come to an equilibrium stage since all the dissolved
oxygen loss is so great and varies so immensely with only small differences
in the weight of sodium sulfite employed that attempting to measure this
rate of loss is impractical (Figures 3 and ‘1).
At first glance, Oxsorbent obtained from the Burrell Corporation,
showed great promise as a substance for the field operation check proced-
ure. Good reproducible data was obtained when a syringe was used to
measure the Oxsorbent . However, additional tests revealed that the
capability of Oxsorbent to remove dissolved oxygen from samples decreased
greatly when the Oxsorbent reagent was momentarily exposed to the air.
The most promising results were obtained with the combination of 2 ml
and 0.25 M manganese sulfate and 2 ml 0.50 M potassium hydroxide. One ml
of each solution produced too slow and too small a DO change (initial
DO of 9.20 ppm changed to 7.85 ppm after 10 minutes, sample temperature
2L1..OC) while two ml of manganese sulfate solution and four ml of potas-
sium hydroxide solution produced too great and too rapid a DO change

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- 12 -
(initial DO of 9.15 ppm changed to 1.80 ppm in 15 minutes, sample tempera-
ture 214.2C). Using a syringe, instead of a pipet, to measure these
reagents did not improve the reproducibility of data. The procedure,
employing 2 ml of each solution, was applied in 17 trials with a
10-minute reaction period and in 18 trials with a 15-minute reaction
period, sample temperature range 23.6C to 25.9C. The standard deviation
of the field operation check method, whether using a 10 or 15 minute
reaction period, was 0.20 (Table 9). These tests were conducted with
the same membrane on the DO probe. When a new membrane was installed
and the field operation check performed twice in triplicate, the change
in DO during a 10-minute reaction period averaged 3.85 ppm, sample
temperature 25.8C. The mean i ppm value (3.60) and the standard deviation
value (0.20) of the proposed 10-minute test are dependent upon the parti-
cular instrument involved and any large differences between temperatures
of samples; therefore, the analyst should establish the ppm value for
his own instrument and in the desired sample temperature span before
employing it in the field. However, mean ppm values obtained under
various conditions are not expected to differ greatly from our mean
value of 3.60 ppm.
In a simulated field evaluation, a triplicate field operation check
required approximately 35 minutes. This time represents the maximum
interval and is greatly reduced by performing the triplicate 10-minute
test simultaneously. In any event, the field operation check requires
less time than a complete calibration check involving the Modified
Winkler Method.

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Conclusions
When properly interpreted, biochemical oxygen demand measurements can
serve as an index to water quality. The Alsterberg (Azide) Modification
of the Winkler Method proved to be an unsatisfactory method for deter-
mining these measurements on the quench water samples tested. However,
before purchasing a DO analyzer the analyst can, if he so desires, judge
for himself the applicability of the Modified Winkler Method by perform-
ing some quick qualitative tests (appendix) on his particular samples.
The Weston and Stack DO Analyzer, Model 300-B, successfully analyzed
quench water samples for their BOD content and a detailed method was
written for the application of this instrument. Included in this detailed
method is the newly developed procedure for an easy and quick field
calibration check on a properly calibrated DO Analyzer.

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References
1. American Public Health Association, American Water Works Association,
and Water Pollution Control Federation. Oxygen (Dissolved), In Standard
Methods for the examination of water and wastewater. 12th Edition.
New York, American Public Health Association, Incorporated, 1965.
p. 405-421.
2. American Society for Testing Materials, Committee D-l9. Dissolved
Oxygen in Industrial Waste Water, D 1589-60. In Manual on industrial
water and industrial wastewater. 2nd ed. Philadelphia, American Society
for Testing Materials, 1966. p. 589-592.
3. Weston and Stack, Incorporated. Instructions for Weston and Stack
Model 300 Dissolved Oxygen Probe. West Chester, Pennsylvania, 1968.
4. Yellow Springs Instrument Company Incorporated. Instruction Manual
for Model 54 Oxygen Meter. Yellow Springs, Ohio, 1968.
5. Theriault, E. J., and P. D. McNamee. Dissolved oxygen in the
presence of organic matter, hypochlorites and sulfite wastes. Public
Health Reports . 48:1363-1377, Nov. 1933.
6. Buswell, A. M., and W. W. Gallagher. The determination of dissolved
oxygen in the presence of iron salts. Industrial and Engineering Chemistry .
15 (11): 1186-1188, 1923.

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I. Tables and Figures
TABLE I
APPLICATION OF ALSTERBERG (AZIDE) MODIFICATION OF
THE WINKLER METHOD TO QUENCH WATER
Sample
Exact site
ml
of
sample
5-day
BODa
Nos.
of collection
diluted
to
one liter
(ppm)
1 Pool of water in residue 50 156
disposal area 156
2 Same 50 148
154
3 Same 25 248
227
4 Same 25 127
292
5 Same 10 117
107
6 Water draining from 50 11.2
trucks, just filled with 11.0
residue
7 Same 50 12.6
9.6
8 Same 10 383
203
9 Same 10 325
198
10 Same 5 24.0
12 4
a Each sample had one initial DO and two final DO. The two final DO values
permitted duplicate BOD results to be reported.
Example: sample #2, initial DO of 10.05 minus final DO values of 0.91
and 0.50 yielded duplicate BOD results of 7.41 ppm and 7.72 ppm.
These results times a dilution factor of 20 equaled BOD values
of 148 ppm and 154 ppm for the original sample.

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TABLE 2
EFFECT OF SAMPLE AGE UPON BOD RESULTSa
b
Sample
Numbers
BOD, ppm (5 day basis)
Determinations initiated Determinations initiated
3’ hours after collection 15 days after collectionc
(Modified Winkler Method) Modified Winkler DO Analyzer
1 127 ‘ .i.9.2 23.6
292 6.4 9.6
2 383 331 59.6
203 330 9.6
3 325 316 57.6
198 311 77.6
2L .O 321 103.6
l2 .i 293 65.6
aSince a DO analyzer was not available during all of the study, a complete
comparison of these results with those of a DO analyzer could not be made.
bThese examples were also used in Table 1 and did not show good agreement.
CSamples stored at room temperature during aging. Values expected to be
about 100 ppm to 300 ppm. Assuming DO Analyzer values are correct, com-
paring values obtained with the two methods indicates the BOD data
employing the Modified Winkler Method is affected by impurities present
in quench water samples.

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TABLE 3
BOD DETERMINATIONS PERFORMED WITH THE ALSTERBERG (AZIDE)
MODIFICATION OF THE WINKLER METHOD AND THE WESTON AND STACK
DO ANALYZER, MODEL 300-B
Sample
Numbers
Exact site
of collection
BOO,
ppm
Modified Winkler
DO Analyzer
1 a
Tap water just
before quenching
process
2.41
3.45
0.15
0.00
2 b
Water draining from
truck employed to
haul quenched residue
119.2
4I. .6
155.0
112.0
3
Sewer directly under
residue hopper
150.8
141.6
165.4
168.0
4 c
Pool between residue
disposal area and
loading zone.
36,0
27.5
143.4
149.0
aAlthough not a quench water sample, the BOO
the possible amount of BOD value that could
being employed. However, when the Modified
the results indicate the value obtained may
results of this sample represent
be contributed by the water
Winkler Method is employed,
be due to interferences only.
bAlthough previous shown with the Modified Winkler Method in Table 1, this
sample was analyzed to show that with either method samples from this
source generally yield BOD data with poor agreement.
cBased upon a definite positive result to a qualitative test, this sample
contained an excessive amount of chlorides.
, . A

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TABLE 4
EFFECT OF NITRIFICATION INHIBITION
AND pH ADJUSTMENT UPON BOD ANALYSIS
Sample
Nos.
BOD, ppm
Before Inhibition
and
I fter Inhibition and
pH Adjustment
Modified Winklerl
DO
Analyzer
pH Adjustment (6.5
Modified Winklerl
to
DO
8.3)
Analyzer
1
119.2
155.0
132.8
130.4
2
150.8
166.4
129.2
126.8
3 b
36.0
143.4
4.4
93,6
14 c
27.2
0.0
2.0
0.0
5 d
35.6
8.2
35.0
30.0
6 e
23.8
0.0
12.2
10.8
Data obtained with the Modified Winkler Method are not to be compared with
data of the DO Analyzer Method and such data does not exclude the possibility
of effects from other parameters.
bm 15 example is the same sample employed as example 4 in Table 3 and
reportedly contains excessive amount of chlorides.
CThjs is same sample as example number 3, except the determination was
initiated 8 days after collection. (Comparing examples 3 and 4 is another
example of effect of sample’s age upon the BOD results).
dNot a quench water sample; however, this sample still illustrates the effects
in question upon the BOD analysis. This is a well mixed sample consisting
of the dilution-water-medium normally employed in the BOD test and rela-
tively large amounts of soil.
e
Not a quench water sample. This sample was decanted from a water-soil
mixture (example no. 5) after most of the soil was allowed to settle.

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TABLE 5
DO ANALYSIS
Type of
Method Employed
Number of a
Determinations
Pooled
Standar
Deviation
d
(S)c
Confidence
Interval
±(l.9 6 )( )s
Weston and Stock
Analyzer
82
0.21
± 0 58 d
Modified Winkler
76 ’j
0i 19
± 1.36
aThIS includes initial and final determinations.
bSampie with less than one ml titrant were omitted.
C .
A pooled standard deviation was computed for all determinations.
It was assumed that there was no statistically significant
difference between initial and final variances, i.e. nomoge—
nelty of the variances was assumed.
dThe absolute value of the difference between duplicated
readings should not exceed l.9G( )(s), or 0.58 ppm, more
bhan 5% of the time. The covariance between the duplicated
readings was ignored.

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— 20 —
TABLE 6
BOD ANALYSIS
Type of
Method Employed
Vs
?la
-i-s
Di1utio
Factor
Confidence Rangec or
± (S) (1.96) (itO)
Weston and Stack
0.30
-t0
+
23.5
Analyzer
Modified Winkler
0.69
40
- I-
5 i t.l
a 1 51 , is an estimate of the standard deviation of differences between two
DO readings (i.e., a single BOD result).
bDjlutjofl factor may vary but for calculation purposes the normal dilution
factor is shown here.
c 95 % confidence limits about a single BUD result, assuming a standard
dilution factor of [ tO or 2.5 percent dilution.

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- 21 -
TABLE 7
STABILITY AND STANDARIZATION OF
SODIUM TUIOSULFATE SOLUTION
Solution
Age of Method of
Solution (days) Standardization
.
Normality
(approx.
a
0.025)
1
0
9
14
potassium dichromate
potassium dichromate
potassium dichromate
O.02’49
0.0248
0.0246
2
1
3
6
potassium dichromate
potassium dichromate
potassium dichromate
0.0248
0.0247
0.0248
3
1
1
2
5
5
potassium dichromate
biniodate
biniodate
biniodate
potassium dichromate
0.0245
0.0247
0.0246
0.0248
0.0247
aEach normality value is the average of 2 to 4 determinations.

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— 22 —
TABLE 8
MATERIALS EVALUATED IN THE DEVELOPMENT OF A PROCEDURE
FOR THE FIELD CALIBRATION AND OPERATIONAL CHECK OF THE
WESTON AND STACK DISSOLVED OXYGEN ANALYZER, MODEL 300
Column A Column B
Mediums having an oxygen Substances that may react with a
solubility different from that definite amount of oxygen, dissolved
of water in water
Ethyl alcohol Aspirin
Acetone Sodium sulfite, solid and in solution
Glycerin Potassium iodide
Sodium nitrite
Prestone
Ethylene glycol Ferrous aminonium sulfate
Ethylene glycol with various Oxsorbent®
amounts of water
Potassium todide—sulfuric acid
Methyl alcohol
Potassium iodide—sulfuric acid—
ferrous sulfate
Sodium thiosulfate
Cupric sulfate
Magnesium sulfate
Potassium permanganate
Potassium dichromate
Manganese sulfate—potassium
hydroxide, solid and in solution

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- 23 -
TABLE 9
REPRODUCIBILITY OF DATA OBTAINED IN THE
PROCEDURE FOR THE FIELD OPERATION CHECK
Statistic
Change in
DO readingb
10 Minute
Reaction period
15 Minute
Reaction period
Ant. Meana
3.60
3.75
Std. Dev.
0.20
0.20
..
Confidence Limit
1-
—0.10
1-
-0.10
aBased upon triplicate observations
bResults are based upon our particular instrument and a definite
temperature range (23.6C to 25.9C).

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- 24 —
400
350
4 i
300
E
0
250
0
0 )
2O0
4-a
E
E 150
0 )
CO
C-,
0) 100
4-a
C ,,
0
50
0
Nitrite concentration, ppm
Figure 1. EFFECT OF NITRITE CONCENTRATION IN ‘OUENCH WATER”
SAMPLES UPON DO ANALYSIS BY MODIFIED WINKLER METHOD.
2 3 4 5 6 7 B 9

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— 25 —
0 1 2 3 4 5 6 1 8
Impurity concentration, ppm
Figure 2.
EFFECT OF SULFITE AND FERROUS IRON
CONCENTRATIONS IN “QUENCH WATER”
SAMPLES UPON DO ANALYSIS, USING
MODIFIED WINKLER METHOD.
22
20
18
16
14
12
10
B
6
4
U,
4 J
L. .
0
4- .
Q)
0
•0
4-.
I-
4- ’
4- ’
E
a)
C.)
a)
4-.
0,
2
0

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- 26 -
14
0)
C O
a)
‘I .-
E
= 10
C
C ,,
I-
a)
4- ,
CO
C,)
a)
4-,
E
a)
I-
2
0
10 8 6 4 2 0
Concentration (ppm) of dissolved oxygen
remaining in BOD bottle
Figure 3. EFFECTS OF VARIOUS WEIGHTS OF SODIUM SULFITE
ON DO DEPLETION IN WATER SAMPLES.
(Sample temp. 24°C)
16

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— 27 —
4- .
4- .
a,
a)
a,
“C
0
0
I .. ,
0
=
E
0.
a,
4 - .
c
0.8
0.7
0.6
0.5
0.4
0.3
0.2
__ 0. 1
0.3
weight (g) of sodium sulfite added to BOO bottle
Figure 4. RATE OF DO DEPLETION IN WATER SAMPLES PRODUCED
BY VARIOUS WEIGHTS OF SODIUM SULFITE.
0
0 0.05 0.1 0.2

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- 28 -
II. MECHANISM OF ALSTERBERG (AZIDE MOD1FICATION OF THE WINKLER METHOD
The Alsterberg (Azide) Modification of the Winkler Method employed
in this study has not been altered from its original concept. The znech—
anisms of this method is presented so that the reader may better under—
stand this report.
The Modified Winkler Method involves the oxidation of manganous by
the oxygen, dissolved in the water, to manganic hydroxide:
MnSO + 2 KOH—..Mn(OH) 2 + K 2 S0 4
2Mn(OH)2 + 02 —a. . 2MnO(OH)2
Which, when acidified, forms manganic sulfate.
MnO(OH)2 + 2H2SOt+ —b. Mn(SO.)2 + 3H20
In the presence of iodide, the manganic sulfate acts as an oxidizing agent,
releasing free iodine.
Nn(S0 .) 2 + 2K1 —4.-MnSO , + K 2 SO + 12
The latter, which is stoichiometrically equivalent to the dissolved oxygen
in the sample, is titrated with O.025N sodium thiosulfate, in the presence
of starch or Thyodene, to a blue end point.
12 + 2Na2S203 — Na:S 0 + 2NaI
One ml of 0.O25 sodium thiosulfate is equivalent to 0.200mg of dissolved
oxygen or if 200 ml of original sample is titrated one ml of titrant is
equivalent to 1 ing/Z (ppm) DO.
Na 2 S 2 O 3 12= n(SO 4 ) E MnO(OH) 2 02

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- 29 -
The Alsterberg (Azide) Modification of the Winkler Method utilizes
sodium azide to reduce nitrites which are commonly found in polluted
waters and interfere in the analysis.
Without sodium azide:
2HN0 2 + 2H1 _ ._ 12 + 2H 2 0 + N 2 0 2
2N 2 0 2 + 2H 2 0 + 02 _— L HNO
With sodium azide:
NaN 3 + H 2 SO —3 NaHSO 4 + HN 3
HN 3 + HNO 2 —9 N + N 2 0 + H 2 0
III. MECHANISM OF WESTON AND STACK (DO) ANALYZER
The Weston and Stack Analyzer, Model 300, is ruggedly constructed,
moisture-proof, and portable. Its probe is constructed of cast-epoxy and
is separated from the sample by a Teflon membrane through which the gases,
dissolved in a sample, can diffuse. The probe is specially designed to
fit into standard BOD bottles and has a built-in agitator which produces a
precise and constant degree of sample turbulence. Within the probe is
a pair of electrodes surrounded by a solution of electrolyte, potassium
iodide.
The oxygen, dissolved in the sample, is reduced at the platinum electrode
cathode:
02 + 2H 2 0 + 4e —1 I4OH
by the 0.578 voltage which results from the cell’s potential energy.
Hydroxide ions flow through the electrolyte and react with the lead anode:
Pb + t OH — PbO + 2H 2 O + 2e

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— 30 —
to produce a flow of electrons. The signal from the probe is directed to
an operational amplifier in the readout instrument. The meter is calibrated
so that each meter readout unit is equivalent to one ppm (mg/9. ) of dis-
solved oxygen in the sample. Changes in the oxygen diffusion rate due to
temperature variation is compensated for by special electronic features of
the analyzer. The instrument is calibrated in a medium similar to the
final sample medium to prevent any change In diffusion rate produced by
dissimilar conditions.
IV. QUALITATIVE TESTS FOR IMPURITIES
These tests may be employed as a quick means of detecting the presence
of oxidizing or reducing substances which will interfere with the Modified
Winkler Method for the determination of BOD.
Although sulfate does not affect the Modified Winkler Method, the
presence of large quantities of it may indicate the possible presence of
sulfite which definitely affects this method. Sulfate is generally de-
tected 1 by adding a portion of barium chloride solution (about lOg to
500 m 2 ) to the sample. A cloudy white solution indicates substances like
sulfate and/or sulfite are present.
Substances, similiar in effect to sulfite, c n be detected by fol-
lowing a procedure similar to the method employed to standardize the
sodium thjosulfate solution utilized in the Modified Wi kler Method. A
small amount of a potassium iodide solution (2 g to 200 m2 ., plus 10 m2.
conc. FIC1, to final vol. of 250 mQ—prepared day to be used) is a Ied to

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- 31 -
the sample. A cloudy white or yellow (color appears after 1 to 2 minutes)
solution indicates that interferences like thiosulfate are present. How—
ever, if the mixture remains clear, sulfite—like interferences may still
be present. Add a small amount of Thyodene powder to the clear mixture and
to a small amount of the potassium iodide solution (may turn pale blue).
Then wfl.h both solutions perform a dropper—titration using potassium di-
chromate solution (2g to 500 mP ) as the titrant. The potassium iodide
solution should turn the yellow color of the dichromate solution with only
1 to 2 drops of titrant. If even only one ppm of sulfite—like material is
present in the sample solution, the solution will turn blue with only I to
2 drops of titrant and several drops will be needed before the sample
solution has the yellow color of the dichromate solution. The pre . ence of
sul,fite—type substances in a sample can produce false negative results with
the tests for interferences similar to nitrite, ferrous iron, ferric iron,
and residual chlorine in effects. However, since no amounts of sulfite—
type materials can be present in a sample, a positive test for these na —
terials eliminatesthe need in performing other tests.
Since nitrite, above 5 ppm in BOD bottles, will interfere with the
Modified Winkler Method, the analyst must detect its presence if he wishes
to use this method for BOD analysis. The test for nitrites descrihed here
is an adaptation of a procedure developed by Saltzman. 2,3 This test in-
volves the addition of a small amount of test solution (10 m acettc acid,
0 g sulfanilic acid, ig l—naphthylanine hydrochloride, dissolved and di-
luted to 250 nQ) to the sample. A red color produced by an azo & e irniL—
cates that substances similar to nitrite are present. A corparisou oF the

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— 32 -
red color to that color produced by a nitrite standard will show the ap—
pro cimate amount of nitrite—type substance present.
Interferences, as ferrous iron, can be detected by a prirtion
of 1,10 phenanthroline solution (5g to 500m9.) to the sample. After a
few minutes,an orange—red complex will indicate the presence of substances
similar to ferrous iron in effect.
Since very large amounts (50 ppm or more in BOD bottle) of ferric
iron or similar substances can also affect the Modified Winkler Method, the
presence of these substances must be determined. The method for this de-
termination involves the addition of a small amount of hydroxylamine solu-
tion (5g to 500mY. ) to the sample port..on, previously tested for ferrous ircri.
After a few minutes the formation of a deeper orange—red complex indicates
the presence of ferric—iron—type materials. A comparison of this color ‘ :ith
the color produced by a ferric iron standard solution will indicate the ap-
proximate concentration of ferric—iron—type substances in the sample.
Since residual chlorine, another interfering substance, dissipates
when samples stand for 1 to 2 hours or they are well aerated, a test for
residual chlorine and similar gases was not established. However, since
these gases can be created during the Modified Winkler application, a
test for their possible creation is discussed here. A free—halogen Lest
paper, ‘ placed just above the sample while 1 or 2 ml of conc. sulfuric
acid is added to the sample, will turn blue in the presence of a halogen
gas. The test paper is prepared by impregnating ordinary filter p er wLth
a potassium iodide solutIon (2g to 200m9 , plus 5m2. conc. H S0 , to final

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33 -
volume of 250 m9 .—prepared day to be used).
These qualitative tests are by no means the only tests available.
Many tests can be employed but the reader is cautioned that qualitative
tests too specific may not reveal what is really desired. Specific tests
may give more detailed knowledge about the sample, however these tests may
not disclose the presence of all the substances that affect the Modified
Winkler Method.

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- 311. -
References (for Qualitative Tests)
1. American Public Health Association, Water Works
Association, and Water Pollution Control Federation.
Sulfate. In Standard Methods for the examination of
water and wastewater. 12th ed. New York, American
Public Health Association, Incorporated, 1965.
p. 287—296,
2. Saltzman, B. E. Colorimetric Micrddetermination of
nitrogen dioxide in the atmosphere. Analytical
Chemistry . 26 (12): 1949—1955, Dec. 1954.
3. American Public Health Association, American Water Works
Association, and Water Pollution Control Federation.
flitrogen (nitrite). ibid., p. 205—208.
4. American Public Health Association, American Water Works
Association, and Water Pollution Control Federation,
Pheriarithroline Method. ibid., p. 156—159.
5. Feigl, F., Free Halogens. In Qualitative analysis by spot
tests. 3d ed. New York, Elsevier Publishing Company, In -.
corporate, 1946. p. 276.

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- 35 —
V. COST OF BOD MET}(ODS
The cost of performing the Alsterberg (Azide) Modification of the
Winkler Method was estimated assuming that: (1) the life expectancy of
the glassware is five years; (2) two weeks of each year are required for
preparation of reagents; (3) eight days per year are holidays; (4)
operation will be continuous; and (5) the sample dilution requirements
are already known.
The cost of performing the BOD analysis, using the Weston and Stack
(DO) Analyzer, Model 300, was estimated assuming that; (1) the life ex-
pectancy of the analyzer and membrane is ten years and one month, respec-
tively; (2) two weeks of each year are required for calibration and in-
stallation, of membranes; and (3) items 3, 4, and 5 for the Nodified
Winkler Method also apply.
Since an analyst’s salary varies with the degree of his training
and experience, the geographic location, and ‘type of employment, the
yearly cost estimates, presented in the following table, have been reported
in terms of labor time.
LABOR TIME AND COST OF EQUIPMENT AND SUPPLIES PER YEAR
Cost of
Type of No. of Hours required for Free chemicals
method Samples triplicate determinations time and eçuip .
Alsterberg (Azide) 1000 1960 none $213.10
Modified Winkler
Weston and Stack 2000 1960 none $213.00
DO Analyzer

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- 36 -
These estimates indicate that (1) the time required for the per-
formance of a sample analysis and (2) the coBt per sample of materials
and equipment is two hours and $0.21 for the Modified Winkler Method
and one hour and $0.11 for the DO Analyzer Method.

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