U.S. ENVIRONMENTAL PROTECTION AGENCY
                NATIONAL EUTROPHICATION  SURVEY
                         WORKING PAPER SERIES
           PACIFIC NORTHWEST ENVIRONMENTAL RESEARCH LABORATORY
                         An Associate Laboratory of the
              NATIONAL ENVIRONMENTAL RESEARCH CENTER - CORVALLIS, OREGON
                                  and
        NATIONAL ENVIRONMENTAL RESEARCH CENTER - LAS VEGAS, NEVADA
AOPO	697.O32

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                                 REPORT
                                   ON
                               LILYU\KE
                           BLUE EARTH COUM7
                               MINNESOTA
                              EPA REGION V
                          WORKING PAPER No,  107
    WITH THE COOPERATION OF THE
MINNESOTA POLLUTION CONTROL AGENCY
              AND THE
     MINNESOTA NATIONAL GUARD
          NOVEMBER, 1974

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1
CONTENTS
Page
Foreword ii
List of Minnesota Study Lakes iv, v
Lake and Drainage Area Map vi
Sections
I. Conclusions 1
110 Lake and Drainage Basin Characteristics 2
III, Lake Water Quality Summary 3
1V 0 Nutrient Loadings 6
V. Literature Reviewed 11
VI© Appendices 12

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11
FOREWORD
The National Eutrophication Survey was initiated in 1972 in
response to an Administration commitment to investigate the nation-
wide threat of accelerated eutrophication to fresh water lakes and
reservoi rs.
OBJECTIVES
The Survey was designed to develop, in conjunction with state
environmental agencies, information on nutrient sources, concentrations,
and impact on selected freshwater lakes as a basis for formulating
comprehensive and coordinated national, regional, and state management
practices relating to point—source discharge reduction and non—point
source pollution abatement in lake watersheds.
ANALYTIC APPROACH
The mathematical and statistical procedures selected for the
Survey’s eutrophication analysis are based on related concepts that:
a. A generalized representation or model relating
sources, concentrations, and impacts can be constructed.
b. By applying measurements of relevant parameters
associated with lake degradation, the generalized model
can be transformed into an operational representation of
a lake, its drainage basin, and related nutrients.
c 0 With such a transformation, an assessment of the
potential for eutrophication control can be made.
LAKE ANALYSIS
In this report, the first stage of evaluation of lake and water-
shed data collected from the study lake and its drainage basin is
documented. The report is formatted to provide state environmental
agencies with specific information for basin planning [ 5303(e)], water
quality criteria/standards review [ 5303(c)], clean lakes [ 5314(a,b)],
and water quality monitoring [ 5106 and §305(b)] activities mandated
by the Federal Water Pollution Control Act Amendments of 1972.

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111
Beyond the single lake analysis, broader based correlations
between nutrient concentrations (and loading) and trophic condi-
tion are being made to advance the rationale and data base for
refinement of nutrient water quality criteria for the Nation’s
fresh water lakes. Likewise, multivariate evaluations for the
relationships between land use, nutrient export, and trophic
condition, by lake class or use, are being developed to assist
in the formulation of planning guidelines and policies by EPA
and to augment plans implementation by the states.
AC KNO WL EDGMENT
The staff of the National Eutrophication Survey (Office of
Research & Development, U. S. Environmental Protection Agency)
expresses sincere appreciation to the Minnesota Pollution Control
Agency for professional involvement and to the Minnesota National
Guard for conducting the tributary sampling phase of the Survey.
Grant J. Merritt, Director of the Minnesota Pollution Control
Agency, John F. McGuire, Chief, and Joel G 0 Schilling, Biologist,
of the Section of Surface and Groundwater, Division of Water Quality,
provided invaluable lake documentation and counsel during the course
of the Survey; and the staff of the Section of Municipal Works, Divi-
sion of Water Quality, were most helpful in identifying point sources
and soliciting municipal participation in the Survey 0
Major General Chester J. Moeglein, the Adjutant General of
Minnesota, and Project Officer Major Adrian Beltrand, who directed
the volunteer efforts of the Minnesota National Guardsmen, are also
gratefully acknowledged for their assistance to the Survey 0

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iv
NATIONAL EUTROPHICATION SURVEY
STUDY LAKES
STATE OF MINNESOTA
LAKE NAME COUNTY
Albert Lea Freeborn
Andrusia Beltrami
Badger Polk
Bartlett Koochiching
Bear Freeborn
Bemidji Beltraini
Big Stearns
Big Stone Big Stone, MN; Roberts,
Grant, SD
Birch Cass
Blackduck Beltrami
Blackhoof Crow Wing
Budd Martin
Buffalo Wright
Calhoun Hennepin
Carlos Douglas
Carrigan Wright
Cass Beltrami, Cass
Clearwater Wright, Stearns
Cokato Wright
Cranberry Crow Wing
Darling Douglas
Elbow St. Louis
Embarass St. Louis
Fall Lake
Forest Washington
Green Kandiyohi
Gull Cass
Heron Jackson
Leech Cass
Le Flomme Dieu Douglas
Lily Blue Earth
Little Grant
Lost St. Louis

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V
LAKE NAME COUNTY
Madison Blue Earth
Malmedal Pope
Mashkenode St. Louis
McQuade St. Louis
Minnetonka Hennepin
Minnewaska Pope
Mud Itasca
Nest Kandiyohi
Pelican St. Louis
Pepin Goodhue, Wabasha, MN;
Pierce, Pepin, WI
Rabbit Crow Wing
Sakatah Le Sueur
Shagawa St. Louis
Silver McLeod
Six Mile St. Louis
Spring Washington, Dakota
St. Croix Washington, MN; St. Croix,
Pierce, WI
St. Louis Bay St. Louis, MN; Douglas, WI
Superior Bay St. Louis, MN; Douglas, WI
Swan Itasca
Trace Todd
Trout Itasca
Wagonga Kandlyohi
Wailmark Chisago
White Bear Washington
Winona Douglas
Wolf Beltrami, Hubbard
Woodcock Kandiyohi
Zunibro Olmstead, Wabasha

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•Butternut
2
Map Location
I
I
\ _
x
LILY LAKE
Tributary Sampling Site
Lake Sampling Site
Sewage Treatment Facility
Direct Drainage Area Limits
440
Indirect
Drainage
Area
0 2 M
I __J.
Scale
(
20’
940 15’
440 05

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LILY LAKE
STORET NO. 2747
I. CONCLUSIONS
A. Trophic Condition:
Survey data show that this very shallow lake is eutrophic.
With a maximum depth of about six feet, it is quite unlikely
that Lily Lake could be anything but eutrophic regardless of
cultural influences.
Of the 80 Minnesota lakes sampled, only 15% had a greater
mean total phosphorus, only 20% had a greater mean dissolved
phosphorus, only 8% had less Secchi disc transparency, only 4%
had more mean chlorophyll a, but 55% had greater mean inorganic
nitrogen.
B. Rate-Limiting Nutrient:
No algal assay sample was collected; however, the lake
data show nitrogen limitation in July and late August, 1972
(N/P ratios were less than 1/1).
C. Nutrient Controllability:
During the sampling year, there was an unexpected loss of
phosphorus from Lily Lake; i.e., about 49% more phosphorus was
measured leaving the lake at the outlet station (A-2; see map,
page vi) than can be accounted for by all measured and estimated
inputs.
The cause of this marked imbalance is not known, but it is
evident that a more intensive study of nutrient sources is needed.

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2
II. LAKE AND DRAINAGE BASIN CHARACTERISTICS
A. Lake Morphometry t :
1. Surface area: 142 acres.
2. Mean depth: 3 feet.
3. Maximum depth: 6 feet.
4. Volume: 426 feet.
5. Mean hydraulic retention time: 20 days.
B. Tributary and Outlet:
(See Appendix A for flow data)
1. Tributaries -
Name ______________ __________
Minneopa Creek
County Ditch #50
Minor tributaries &
immediate drainage —
Totals
2. Outlet -
Minneopa Creek
C. Precipitation***:
1. Year of sampling: 29.7 inches.
2. Mean annual: 25.4 inches.
t Schilling, 1974.
* Drainage areas are accurate within ±5%; mean daily flows are accurate
within ±10%; and ungaged flows ar accurate within ±10 to 25% for
drainage areas greater than 10 mit.
** Includes area of lake.
See Working Paper No. 1 , “Survey Methods”.
Drainage area*
30.8 mi 2
10.4 mi 2
.2
2.2 mi
43.4 mi
Mean flow*
7.6 cfs
2.5 cfs
0.5 cfs
10.6 cfs
43.6 mi 2 ** 10.6 cfs

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III.   LAKE WATER QUALITY SUMMARY
      Lily Lake was sampled two times  during  the  open-water season
  of  1972 by means  of a  pontoon-equipped  Huey helicopter.   Each  time,
  near-surface  samples for physical  and chemical  parameters were
  collected from a  single station  on the  lake (see  map,  page vi).   Also,
  during  each visit a single sample  was collected for  phytoplankton
  identification and enumeration,  and  a separate  sample  was taken for
  chlorophyll a^ analysis.   Lily Lake was  not  sampled a third time,
  and  no  sample was collected for  algal assays.
      The results obtained are presented  in full  in Appendix B and  are
  summarized  below.   The values presented in  these  summary  tables are
  not  volume-weighted; nonetheless,  they  are  useful as a general guide
  to differences in water quality  at the  different  sampling times.
      A.   Physical  and chemical  characteristics:
                                                 Values
                                      1st Sample            2nd Sample
          Parameter                   (07/01/72)            (08/30/72)
          Temperature (Cent.)           24.2                  22.0
          Dissolved  oxygen (mg/1)       10.6                  9.2
          Conductivity (ymhos)         455                   610
          pH  (units)                      8.9                  8.6
          Alkalinity  (mg/1)            131                   236
          Total  P (mg/1)                  0.374                0.840
          Dissolved  P (mg/1)              0.142                0.453
          N02 + N03  (mg/1)                0.080                0.150
          Ammonia fmg/1)                  0.060                0.180
          Chlorophyll  a  (yg/1)         188.4                 269.9
          Secchi  disc Tinches)          16                    6

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4
Mm. for Max. for Mean for
Parameter all samples all samples all samples
Temperature 22.0 24.2 23.1
Dissolved oxygen 9.2 10.6 9.9
Conductivity 455 610 533
pH 8.6 8.9 8.8
Alkalinity 131 236 184
Total P 0.374 0.840 0.607
Dissolved P 0.142 0.453 0.297
NO ÷ NO 0.080 0.150 0.115
AJonia 0.060 0.180 0.120
Chlorophyll a 188.4 269.9 229.1
Secchi disc 6 16 11
B. Biological characteristics:
1. Phytoplankton -
Sampling Dominant Number
Date Genera per ml
07/01/72 1. Oscillatoria 24,728
2. Scenedesnius 7,817
3. Flagellates 6,362
4. Cyclotella 4,000
5. Gloeocapsa 3,635
Other genera 14,913
Total 61 ,455
08/30/72 1. Cyclotella 16,362
2. Melosira 11,544
3. Oscillatoria 9,273
4. Gloeocapsa 7,273
5. Nitzschia 5,273
Other genera 9,455
Total
59,180

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5
2. Chlorophyll a -
(Because of instrumentation problems during the 1972 sampling,
the following values may be in error by plus or minus 20
percent.)
Sampling Station Chlorophyll a
Date Number ( pg/i )
07/01/72 01 188.4
08/30/72 01 269.9

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6
IV. NUTRIENT LOADINGS
(See Appendix C for data)
For the determination of nutrient loadings, the Minnesota National
Guard collected monthly near-surface grab samples from each of the
tributary sites indicated on the map (page vi), except for the high
runoff months of March and May, when two samples were collected, and
the colder months when samples were omitted at two stations because
of low flows. Sampling was begun in October, 1972, and was completed
in September, 1973.
Through an interagency agreement, stream flow estimates for the
year of sampling and a “normalized” or average year were provided by
the Minnesota District Office of the U.S. Geological Survey for the
tributary sites nearest the lake.
In this report, nutrient loads for sampled tributaries were deter-
mined by using a modification of a U.S. Geological Survey computer
program for calculating stream loadings . Nutrient loadings for unsam-
pied “minor tributaries and immediate drainage” (“ZZ” of U.S.G.S.) were
estimated by using the nutrient loads, in lbs/mi 2 /year, in County Ditch
#50 at station B-i and multiplying by the ZZ area in mi 2 .
The operator of the Lake Crystal wastewater treatment plant provided
monthly effluent samples and corresponding flow data.
* See Working Paper No. 1.

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7
A. Waste Sources:
1. Known municipal -
Pop. Mean Receiving
Name Served Treatment Flow (mgd) Water
Lake Crystal 1,626* Trickling 0.220 Lily Lake
filter
2. Known industrial - None
* 1970 Census.

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8
B. Annual Total Phosphorus Loading - Average Year:
1. Inputs —
lbs P/ % of
Source yr total
a. Tributaries (non-point load) -
Minrieopa Creek 2,810 30.0
County Ditch #50 840 9.0
b. Minor tributaries & immediate
drainage (non-point load) - 180 1.9
c. Known municipal -
Lake Crystal 4,490 58.9
d. Septic tanks — Unknown
e. Known industrial - None - -
f. Direct precipitation* - 20 0.2
Total 8,340 100.0
2. Outputs -
Lake outlet - Minneopa Creek 12,460
2. Net annual P loss - 4,120 pounds
* See Working Paper No. 1.

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9
C. Annual Total Nitrogen Loading - Average Year:
1. Inputs —
lbs N! % of
Source yr total
a. Tributaries (non-point load) —
Minneopa Creek 136,350 64.8
County Ditch #50 44,990 21.4
b. Minor tributaries & immediate
drainage (non-point load) - 9,520 4.5
c. Known municipal -
Lake Crystal 15,040 8.6
d. Septic tanks - Unknown
e. Known industrial - None - —
f. Direct precipitation* - 1,370 0.7
Total 207,270 100.0
2. Outputs —
Lake outlet - Minneopa Creek 218,170
3. Net annual N loss - 10,900 pounds
* See Working Paper No. 1.

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10
D. Mean Annual Non-point Nutrient Export by Subdrainage Area:
Tributary lbs P/mi 2 /yr lbs N/mi 2 /yr
Minneopa Creek 91 4,427
County Ditch #50 81 4,326
E. Yearly Loading Rates:
In the following table, the existing phosphorus loading
rates are compared to those proposed by Vollenweider (in press).
Essentially, his “dangerous” rate is the rate at which the
receiving waters would become eutrophic or remain eutrophic;
his “permissible” rate is that which would result in the
receiving water remaining oligotrophic or becoming oligotrophic
if morphometry permitted. A mesotrophic rate would be con-
sidered one between “dangerous” and “permissible”.
Total Phosphorus Total Nitrogen
Units Total Accumulated Total Accumulated
lbs/acre/yr 58.7 loss 1,459.6 loss
grams/m 2 /yr 6.58 - 163.6
Volle weider loading rates for phosphorus
(g/m /yr) based on mean depth and mean
hydraulic retention time of Lily Lake:
“Dangerous” (eutrophic rate) 0.78
“Permissible” (oligotrophic rate) 0.39

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11
V. LITERATURE REVIEWED
Anonymous, 1973. Wastewater disposal facilities inventory. MPCA,
Minneapolis.
Schilling, Joel, 1974. Personal communication (morphometry of
Lily Lake). MPCA, Minneapolis.
Volleriweider, Richard A., (in press). Input-output models. Schweiz.
A. Hydrol.

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VII. APPENDICES
APPENDIX A
TRIBUTARY FLOW DATA

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TRIBUTARY FLOW INFORMATION FOR MINNESOTA 10/30/74
LAKE CODE 2747 LILY LAKE
TOTAL DRAINAGE AREA OF LAKE 43.60
SUB—DRAINAGE NO MAL1ZED FLOWS
TRIBUTARY AREA JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC MEAN
2747A 1 30.80 0.42 0.59 6.14 29.20 14.80 20.70 8.1 4.13 3.15 2.06 1.67 0.75 7.64
274742 43.60 0.60 0.85 8.97 41.90 20.90 27.00 11.40 5.77 4.28 2.85 2.36 1.03 10.65
274781 10.40 0.15 0.18 2.64 9.31 4.80 7.06 2.65 1.18 1.04 0.60 0.46 0.25 2.52
274712 2.20 0.02 0.02 0.37 1.87 1.02 1.59 0.58 0.34 0.25 0.12 0.08 0.04 0.52
SUMMARY
TOTAL DRAINAGE AREA Of LAKE • 43.60 TOTAL FLOW IN = 128.41
SUM OF SUB—DRAINAGE AREAS — 43.40 TOTAL FLOW OUT = 127.91
MEAN MONTHLY FLOWS AND DAILY FLOWS
TRIBUTARY MONTH YEAR MEAN FLOW DAY FLOW DAY FLOW DAY FLOW
2747A1 10 72 2.03 15 1.20
11 72 4.73 5 11.00
12 72 1.40 4 2.40
1 73 2.23 13 0.80
2 73 2.35 4 4.80
3 73 24.40 3 12.00
4 73 12.00 24 9.40
5 73 17.30 16 12.60 31 24.00
6 73 6.2!
7 73 0.82 12 0.70
8 73 0.33
9 73 0.41 2 0.40
274742 10 72 2.81 15 1.70
11 72 6.66 5 16.00
12 72 1.94 4 3.30
1 73 3.19 13 1.20
2 73 3.39 4 4.00
3 73 35.70 3 18.00
4 73 17.20 24 13.00
5 73 24.40 16 18.00 31 34.00
6 73
7 73 1.14 12 0.90
8 73 0.46
9 73 0.56 2 0.55

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TRIBUTARY FLOW INFOp MATION FOR MINNESOTA
10/30/74
LAKE CODE 2747
LILY LAKE
MEAN MONTHLY FLOWS AND DAILY FLOWS
TRIRUTARY
MONTH
YEAR
MEAN FLOW
DAY
FLOW DAY FLOW DAY FLOW
2747B1
2747Z7
10
11
12
1
2
3
4
5
6
7
A
9
10
11
1?
1
?
3
4
5
6
.7
8
9
72
72
72
73
73
73
73
73
73
73
73
73
72
72
7
73
73
73
73
73
73
73
73
73
0.59
1.29
0.46
0.80
0.72
10.50
3.82
5.62
2.12
0.27
0.09
0.14
0.11
0.22
0.07
0.11
0.08
1.47
0.77
1.19
0.48
0.06
0.03
0.03
5
5
4
13
4
3
24
16
12
2
15
5
4
13
4
3
24
16
12
2
0.40
3.00
0.80
0.30
0.90
5.40
3.00
4.10
0.22
0.05
0.03
31
7.80
0.14
0.06
0.50
0.10
0.04
0.10
0.80
0.60
0.90
31
1.60

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APPENDIX B
PHYSICAL and CHEMICAL DATA

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APPENDIX C
TRIBUTARY and WASTEWATER
TREATMENT PLANT DATA

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STORET RETRIEVAL DATE 74/10/30
274741 LS2747A1
44 07 30.0 094 15 00.0
M!NNEOPA CREEK
27 15 MANKATO WEST
I/LILY LAKE
Co HWY 111 RDG 1 MI UPSTREAM OF LILY LK
1 IEPALES 2111204
4 0000 FEET DEPTH
00630 00625 00610 00671 00665
DATE TIME DEPTH NO?F NO3 TOT KJEL NH3—N PHOS—DIS PHOS-TOT
FROM OF N—TOTAL N TOTAL ORTHO
TO OAY FEET MG/L MG/L MG/L MG/L P MG/L P
72/10/15 14 00 1.560 0.840 0.140 0.048 0.107
72/11/05 10 00 5.200 2.520 0.064 0.089 0.230
7?/!2/04 0.028 0.990 0.054 0.005K 0.011
73/03/03 10 00 5.300 3.990 0.590 0.410 0.700
73/03/?3 14 00 11.000 2.900 0.350 0.046 0.110
73/04/24 14 50 16.400 1.150 0.020 0.042 0.100
73/05/16 08 30 15.200 1.600 0.115 0.050
73/05/31 09 00 17.000 1.400 0.066 0.029 0.155
73/07/12 10 00 5.900 1.540 0.147 0.076 0.135
73/09/07 14 30 0.500 3.400 0.147 0.210 0.345
K VALUE KNOWN TO BE LESS
THAN INDICATED

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STORET RETRIEVAL DATE 74/10/30
2747A? LS2747A2
44 07 00.0 094 13 00.0
MINNEOPA CHEEK
27 15 MANKATO WEST
0/LILY LAKE
Co HWY 20 9 DG AT OUTLET OFLILY LAKE
1 1EPALES 2111204
4 0000 FEET DEPTH
00630 00625 00610 00671 00665
DATE TIME DEPTH N02&N03 TOT KJEL NH3—N PHOS—DIS PHOS—TOT
FROM OF N—TOTAL N TOTAL ORTHO
TO DAY FEET MG/L MG/L MG/L MG/L P MG/L P
72/10/15 13 30 0.016 4.485 0.102 0.084 0.165
72/11/05 10 00 1.110 3.600 0.067 0.132 0.460
72/12/04 15.500 0.100K 0.042 0.042 0.063
73/01/13 11 00 9.450 7.300 4.800 1.700 2.100
73/02/04 09 00 5.000 4.900 2.600 0.970 1.300
73/03/03 11 30 5.600 2.900 0.890 0.310 0.430
73/03/23 13 30 9.200 5.100 0.600 0.273 0.400
73/04/24 20 00 10.000 2.600 0.026 0.044 0.220
73/05/16 08 00 10.300 3.100 0.098 0.066 0.970
73/05/31 09 30 14.000 0.805 0.250 0.037 0.13S
73/07/1? 11 08 0.026 5.500 0.096 0.350 0.720
73/09/02 14 50 0.021 0.160 0.420 1.050
K VALUE KNOWN TO HE LESS
THAN INDICATED

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STORET RETRIEVAL DATE 74/10/30
?747fl Ls2747R1
44 06 30.0 094 14 00.0
UNNAMEI) STREAM
27 5 MANKATO WEST
T/LILY LAKE
Co HWY 6 XING NW OF LK CP YSTAL
1 1EPALES 2111204
4 0000 FEET DEPTH
00630 00625 00610 00671 00665
DATE TIME DEPTH N02&N03 TOT KJEL NH3—N PHOS—DIS PHUS-TOT
FROM OF N-TOTAL N TOTAL ORTHO
TO DAY FEET MG/I MG/I MG/L MG/L P MG/L P
72/10/15 13 45 1.370 0.400 0.031 0.034 0.060
72/11/05 10 00 3.200 0.150 0.048 0.115 0.160
7?/12/04 12.800 0.308 0.048 0.063
73/03/03 09 00 0.357 4.500 0.900 0.022
73/03/23 13 00 14.000 4.400 1.105 0.086 (1.155
13/04/24 09 00 17.000 0.260 0.040 0.084 0.185
13/05/16 09 00 15.200 4.300 0.100 0.120 0.600
73/05/31 09 15 19.000 0.032 0.026 0.105
71/07/12 10 30 6.400 2.200 0.096 0.056 0.200
73/09/0? 15 00 0.850 2.100 0.176 0.105 0.flO

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