?:-.«^*ii««* »*t . _.»«•_• . -vw*~ • J ..-•'. Agency Wohington. DC 2O460 &EPA Pesticide Fact Sheet Name of Chemical: Acephate Reason for Issuance: issuance of a Registration Standard Date Issued: 10/37 Fact Sheet Number: 140 1. Description of Chemical Generic Name: 0,S-dimethyl acetylphosphoramidothioate Common Name: Acephate Trade Name: Orthene EPA Shaughnessy Code: 103301 Chemical Abstracts Service (CAS) Number: 30560-19-1 Year of Initial Registration: 1974 Pesticide Type: Insecticide Chemical Family: Organophosphate U.S. and Foreign Producers: Chevron Chemical Co. (U.S.A.) 2. Use Patterns and Formulations Application Sites: Agricultural crops; ornamentals (field grown, greenhouse, and home garden); lawns and turf; pasture and rangeland; forestry; indoor homeowner use on houseplants; and commercial appli- cator use in residential and commercial buildings including food processing establishments. Types and Methods of Application: Aerial; ground; direct injection into tree trunks; dip treatment (ornamentals); soil incorporated; and sprinklers. Type of Formulations: Granular, pressurized liquid, soluble concentrates (both liquids and solids), and cartridge. 3. Science Findings Summary Science Statement: Acephate has a relatively low acute toxicity to laboratory animals through the oral, dermal, and inha- lation routes of exposure. Based on the available evidence, i.e., findings from the mouse oncogenicity study, and the mutagenicity assays, the Agency has classified the chemical as a category C carcinogen (a possible human carcinogen). The mouse oncogenicity study indicated a statistically significant increase in the propor- tion of liver adenomas/carcinomas and hyperplastic nodules occurred only in the high dose (1000 ppm) females and only at the time of terminal sacrifice. The EPA Guidelines for carcinogenic ------- —2— risk assessment (FR September 24, 1986) were followed for the evalu- ation and the classification of the oncogenic effect of acephate. Following the guidance set forth in the EPA guidelines, the mouse oncogenic response was considered as “limited evidence.” The available data are not sufficient to enable the Agency to accurately assess the potential risk to humans from this oncogenic effect resulting fran exposure to acephate. The data gaps include residue reduction studies, exposure studies, usage data, a dermal penetration study, a glove permeability study, and reentry data. The available rat reproduction study showed reproductive effects at 50.0 ppn, the lowest dose tested. A new rat reproduction study is needed to determine the no—observable—effect level (NOEL) for the6e effects and to enable the Agency to assess the potential risk to humans resulting from exposure to acephate. Methylthioacetate (MTA) occurs as an impurity in the current registered technical material. The available data suggest that the MTA, despite its generally low acute toxicity, may pose a hazard to the optic tract and pituitary gland in rabbits and other mammals at low doses. Data were not provided to demonstrate a NOEL for lesions at these target organs. Since visual impairment is inherently difficult to diagnose in animals, it is possible that this effect occurred in other studies but was not detected. In addition, a mutagenic effect was seen in the mouse lyinphana assay in the activated system. 1 Je to the insufficiency of the submitted data to explain the toxic and mutagenic potential of MTA, the Agency requires that additional studies be performed. Methamidophos, the cholinesterase—inhibiting metabolite of acephate, is also an insecticide in its own right, and as such, was assessed under a separate Registration Standard issued for the chemical in September 1982. Several of the data gaps identified in that standard have been fulfilled. It is highly toxic by both the oral and dermal routes (Toxicity Category I). Results of two oncogenicity studies show that methamidophos was not oncogenic in rats at dose levels of 2,6,18 and 54 ppi nor in mice at dose levels of l,5,and 25 ppTi. The available teratogenicity studies show that it is not teratogenic to rats or rabbits. The chemical was negative for acute delayed neurotoxicity in the submitted study on hens. The lowest effect level (LEL) for cholinesterase inhibition activity was determined to be 2 pçm (0.05 mg/kg/day) in both the 1—year dog study and the 2—year rat study. Data gaps for methaniidophos include a rat reproduction study and mutagenicity studies. Chemical Characteristics Physical State: Solid Color: White Odor: Strong, pungent, mercaptan-type Boiling Point: N/A ------- —3— Melting Point: 82—89 °C (97% technical) Flawriability: N/A Solubility in Water: High solubility 65%) Toxicology Characteristics: ACEPHATE : o cute Oral — Rat: 945 mg/kg (male); 866 mg/kg (female) Toxicity Category III o Pcute t rmal — Rabbit: > 10,000 mg/kg (male) Toxic ity Ca tego y III o Pc ute Inhalation — Rat: > 61.7 mg/kg (male and female) Toxicity Category IV o Pcute t layed t urotoxicity — Hen: gative at 785 mg/kg of body ight o use (Yicogenicity: Female mice fed 1000 p xn of technical acephate (highest dose tested) had a statistically significant higher ircider*e of hepabxellular cattin as (15.8%) and hyperplastic nodules (19.7%) than did the controls. o Rat C cogenicity: Nt orcogenic to male and female rats under the conditions of the sUx y; highest dose tested s 700 ppn (35 mg/ kg). o Rat throri ic Feed ing: LEL = 5 p n (0.25 mgi’ kg) based on the inhibition of cholinesterase activity in pla&na, RBC, and brain. ) D g Chronic Feeding: I .XJEL = 30 pixn (0.75 mg/kg) based on the inhibition of pla na, RBC, and brain cholinesterase activity. ? DEL = > 100 p xn (2.5 mg/kg) for systemic toxicity. o Rabbit ratogenicity: t fetotoxic or teratogenic at 10 mg/kg (highest dose tested). o Rat ratogenicity: Nt teratogenic at 200 mg/kg (highest dose tested). o ttitageriic ity: The available stu ies irid icate that acephate can indice gene mutations, D N A repair, and sister chxxxnatid exchanges. Ho ver, in vivo st ies did r t indicate that these effects and structural chranosa aberrations are prockced at a detectable level in an intact mam al ian system. ------- -.4- o Rat Reprodution: Various reprod ctive eff ts (low pregnarcy rate, high loss of total litters, high fetal losses, decreased size and weight of total litters, arid dereased n nber of live fetuses) were observed at the lowest dose level tested, which was 50.0 ppn of tehnical acephate (93% acephate). ME’rHYLTH IC CEThTE ( MrA) : o ?cute Dermal - Rabbit: 1720—2820 n f kg; Toxicity Category 11—111. Clinical signs ircluded irreversible abserce,/di,ninution of pupillary light reflex arid apparent blindness. o k ute Inhalation — Rat: 3.47 mg/L; Toxicity Category III. o Skin Irritation — Rabbit: 2.6 Primary Irritation Seore; Toxicity Category III. o Skin Sensitization — Guinea Pig: Nnsensitizing and r nirritating; dose level tested was 0.3 ml (0.3 g). o Eye Irritation — Rabbit: Toxicity Category III; dose level tested was 0. 1 mL of 93. 5% MT . o Mutajenic ity - Mouse Lymphana Assay: Mutajen ic to lymphana cells in the activated system but not in the nonac tivated system; levels tested were 1—10,000 uqjml (activated) and 10—5000 ug/ml (r nactivated). Physiological and B ixhemical Behavioral Characteristics : Translcxation: The available plant metabolisn studies show that acephate residues are readily absorbed by the roots and trarisl ated throujbout the plant. wever, data show that acephate does r t accunulate in carrt plants rotated in acephate-treated soil or in fish, daphnia, or diatans. Me hanisn of Pesticidal k tion: cephate is a contact arid systemic insec tic ide. As an or9anophosphate, acephate exerts its toxic action by inhibitir certain important enzymes of the nervous system (cbolinesterase). Metaboli&n arid Persisterce in Plants arid Animals: The netaboli n of acephate in plants arid animals is adequately understood. Available data s w that the residues in or on plants resulting fran acephate use may be laz ely or wholly intact acephate arid its netabolite, neth nidopbos. Available animal metabolisn data show that most of the radiolabeled material is rapidly eliminated fran the body arid that a majority of the material is excreted in the urine. ------- —5— Methamidophos is not the major metabolite in ruminants. About 80 pez:cent of the radiolabeled material in the urine was ass’xiated with urchanged acephate arid less than 10 petcent with the metabol ite 0, S-d imethylphosphorothioate. Most of the methamidophos formed is probably eliminated arid exc re ted in the urine as 0, S—d irne thylphosphorthioate. Environmental Characteristics : We to its rapid leaching behavior, acephate has the ptential for ground water contamination. Available data are insuf- ficient t’) fully assess this potential. Pertinent data (mobility, phot x ejradation , metabolian, arid dissipation) — are being required under the Pcephate ReQ istration Standard on an accelerated basis. Available soil metabolisii studies show that acephate dissipates rapidly with half—lives of < 3 and 6 days in aerobic and anaerobic soils, respectively. The major meta- bolite was CO 2 in both types of soil. The available leaching data irclude a soil thin—layer chr iiiatography (TLC) study arid a soil column study. Results of these studies indicate that acephate is mobile in most soils but that aged acephate residues (exc luci i rxj acepha te a rid its degrada te ne tham idophos) are immobile in sandy loam soil. Apparently most of the applied acephate arid the dejradate methamidophos degrade to immobile ccxnpounds in 20 days. Ecological Characteristics : o Avian Oral Pcute Pxicity: 350 mg/kg (mallard) and 140 mg/kg (pheasant) o Avian Dietary Toxicity: > 5000 ppe (mallard) and 1280 p xn (bobwhite) o Fish Pcute Toxicity: > 1000 pp (rainb trout) arid > 1000 p n (bluegill sunfish) o Freshwater Invertebrate P ute Toxic i ty: > 1000 p n (thiror inus) and > 100 (G ririarJs) o Avian Repradu tion: JEL = > 5 p n but < 20 p xn for mallard arid NCiEL = > 20 ppn but < 80 p xn for bobwhite. o Honey Bee ute Toxicity: 1.2 ug/bee. Based on these studies, acephate is mx1erately toxic to avian species, practically nontoxic to freshwater fish arid freshwater invertebrates, arid highly toxic to honey bees. ------- —6— However, acephate’s metabolite, methamidophos, has been shown to be very toxic to birds. Therefore, additional testing (residue monitoring studies) are being requested to complete a hazard assessment for the multiple—application, high—use rate field crops. Appropriate labeling for the protection of endangered species determined to be in jeopardy from use of acephate on forests, range and pastureland, soybeans, and cotton have been developed by the Agency and were imposed under PR Notices 87—4 and 87—5. Tolerance Assessment : Refer to Attachment A for the list of currently established tolerances for acephate as well as the tolerance changes to be initiated by the Agency. To achieve compatibility with the maximum residue levels of the Codex Alimentarjus Commission, the following revisions in 40 CFR 180.108, 21 CFR 561.20, 40 CFR 180.315, 21 CFR 561 .277, and 21 CFR 193.10 are to be initiated by the Agency. o 40 CFR 180.108 and 21 CFR 561.20 The acephate tolerances currently established under these sections are to be expressed in terms of only acephate se, with references to 40 CFR 180.315 and 21 CFR 561 .277 indicating that tolerance for the metabolite methamidophos are also in effect. o 40 CFR 180.315 and 21 CFR 561 .277 The methamidophos tolerances currently established under these sections are to be divided into parts (a) and (b) where (a) includes (1) tolerances reflecting uses of methainidophos and (2) tolerances where both acephate and methamidophos formulations are used on the same crop and (b) includes tolerances reflecting uses of acephate formu- lations alone, i.e., residues of methamidophos resulting from the metabolism of acephate. o 21 CFR 193.10 These food additive tolerances reflecting crack and crevice treatment in food-handling facilities are to be expressed in terms of only acephate se, i.e., based on the avail- able data. No residues of the metabolite methamidophos are expected to occur (< 0.001 ppn) in or on these foods. Also, such a change in the residue definition would require deletion of the paragraph (d)(8) of 40 CFR 180.3, which states that methamido— phos residues may not exceed the higher of the two tolerances estab- lished for the use of acephate or methainidophos as a pesticide. ------- —7— The tolerance for acephate residues in milk has been found likely to be exceeded if maximum levels of spent mint hay or grass hay are included in the dairy animal diet. Labeling restrictions prohibit- ing the feeding of spent mint hay and grass hay to dairy animals are being imposed under this Standard for the use of acephate on pasture, rangeland, peppermint, and spearmint. If the registrant elects to submit additional residue data to support a lower toler- ance for grass and grass hay, or to support a longer pregrazing or preharvest interval for dairy animals, the labeling restriction for use on pasture and rangeland would be imposed as an interim precautionary measure pending submittal and evaluation of these data. Available data are not sufficient to conduct a full tolerance assessment. Data gaps exist for magnitude of residue studies, residue storage stability studies, a dairy cattle feeding study, a rat reproduction study, and a rat feeding study. 4. Summary of Requlatory Position Arid Required Unique Labeling Registration of current registered uses of acephate is to be continued. Additional data to allow the Agency to better define the dietary, occupational, and dc nestic exposure risks frai’ the registered uses of the chemical are being required. Once the Agency has evaluated these data, it will determine whether the chemical should be placed in Special Review or returned to the normal registration process. Pending submittal and evaluation of these data, no additional tolerances, including temporary tolerances, will be established for acephate and no new uses will be registered, i.e., uses that would result in an increase in the current exposure to humans or in new exposure to humans. As interim measures to reduce exposure pending submittal and evaluation of the additional studies specified above, the following restrictions are being imposed or, in the case of the last restriction concerning danestic use, continued: A reentry interval of 24 hours for fieldworkers; the use of protective clothing, including chemical—resistant gloves, long—sleeved shirts and long—legged trousers, shoes and socks by mixer/loaders, applicators, and early reentry workers who may be exposed to treated plant surfaces within 24 hours of acephate application; dairy animal feeding restrictions as described above under Tolerance Assessment ; and, for dciiiestic use, the restriction not to allow children or pets on treated surfaces until sprays have dried. ------- -8- The 24-t ur reentry interval is being iirip sed for the use of acephate on aricultural crops, cuui etcially grown orrtamentals, in c tr ercial or governmental forestry seed production, and in greenhoises. As described above under Ecological Characteristics , the Agercy has impsed labeling restrie tions for the protection of endangered species determined to be in jeopardy froi use of acephate. 5. Sumary ot Major Data Gap Toxicology Date Due - Acephate Rat Repr s tion 39 Months 21—Day Inhalation 6 Months (Pr t col) Rat Feeding Study 6 Months (Protocol) — Methylthioacetate (M ) Acute Oral (Rat and Rabbit) 9 Months Acute Dermal ( Final Report — Rabbit) 9 Months Acute Inhalation 9 Months 90-Day Dermal (Rabbit) 15 Months Mutagenicity 12 Months Envirori 1erital Safety Avian Residue Monitoring 6 Months (Protocol) Environmental Fate Soil Ph )tx egradation 9 Months Anaerobic Aquatic Metabolisn 27 Months MsrptioiVDesorptiofl 12 Months Soil Dissipation - Field 27 Months Irrigated Crop 39 Months Confined Rotational Crop 39 Months Spray Drift 6 Months Exposure Applicator (Outd r and Indxr) 6 Months (Protocol) Indoor Inhabitants 6 Months (Protocol) Glove Permeability 6 Months (Protocol) Residue Ch istry Storage Stability 24 Months Magnitude of Residues 24 Months Dairy Cattle Feeding 18 Months Tobacco Residue 24 Months ------- —9— Benefits Usage 6 Months Use-Related Exjxsure 6 Months 6. Contact Person at EPA Wil1i H. Miller (RI 16) Insecticide—RodentiCide Brar h (TS—767C) 401 M Street S . Washington, 1X 20460. DISCLA]JIER: The infoz ation presented in this Chemical Infotniation Fact Sheet is for informational putpses only and may r t be used to fulfill data requirements for pestic ide registration and reregistration. ------- Attach Tolerance Changes Reflecting the Recc . led Change in Residue Definition Acephate* Methainidophos Established Recommended Established Recommended Commodity Toleranpe (ppm)a Tolerance (ppm)b Tolerance (ppm)C Tolerance (ppm)C :;eans (succulent and dry forms) 3(1) 3 1. ‘ russe1s sprouts 3(0.5) 3 1 1 •auliflower 2(0.5) 2 1 1 elery 10(1) 10 1 1 .ottonseed 2 2 0.1 0.5 :ranberries 0.5(0.1) 0.5 0.1 0.1 0.1 0.01 ? f, meat, and meat byproducts’ 0.1 0.1 0.01 ;rass (pasture and range) 15 15 3 Grass hay 15 15 3 ettuce (head) 10(1) 10 le 1 Iilk 0.1 0.1 0.05 lint hay 15(1) 15 1 eanuts 0.2 0.2 0.1 :‘eanuts hulls 5 5 —— 1.5 ‘eppers 4(1) 4 l 1 .oybeans 1 1 0.2 ottonseed hulls 4 4 0.1 ottonseed meals 8 8 2.5 3oybean meals 4 4 2 ‘rocessed foodsh 0.02 0.02 Expressed in terms of combined residues of acephate and methamidophos. If specified, limits of methamidophos are given parenthetically. Expressed in terms of acephate se only. Expressed in terms of only methamidophos r se. ‘ Included are cattle, goats, hogs, horses, poultry, and sheep. The methamidophos tolerance covers all types of lettuce (head and leaf). The methamidophos tolerance covers all types of peppers. g Feed additive tolerances in 21 CFR 561.20 (acephate) and recommendations for inclusion under 21 CFR 561.277 (methamidophos). Food additive tolerance reflecting spot and crack and crevice treatment of food areas of food handling establishments (21 CFR 193.10). There is no expectation of methamidophos residues in such foods (residues were nondetectable, < 0.001 pp , in all cases). * This table does not include the tolerance of acephate in/on macadamia nuts (.05 ppn), which was established subsequent to completion of the evaluation needed for this Document. Acephate is not currently registered for use on macadamia nuts. ------- |