United States Environmental Protection Agency Office of Pesticides and Toxic Substances Office of Pesticide Programs (TS-766C) Washington, DC 20460 cvEPA Pesticide Fact Sheet Name of Chemical: Dodine Reason for Issuance: Registration Standard Date ISSUed: February 1987 Fact Sheet Number: 135 1. Description of the Chemicals Chemical Name: Dodecylguanidine Cannon Name: Brand Names: CAS Registry Number: EPA/OPP Pesticide Chemical Code: Bnpirical Formula: Pesticide Type: Major U.S. Producers: acetate Dodine Doguadine, Tsitrex, Dodine acetate, CL 7521, Dodecylguanidine monoacetate, AC 5223, Cyprex, Melprex, Carpene, Curitan, Syllit, Venturol, and Vondodine 2439-10-3 044301 Dodecylguanidine Dodecylguanidine hydrochloride terephthalate (DGT) (DGH) Cytox 2013 Durotex 7487-A 13590-97-1 044303 C13H30N3C1 Fungicide and Industrial industrial biocide/ biocide/ preservative preservative 19727-17-4 044302 American Cyanamid, Onyx Chemical, Aceto Chemical Betz Not Available Industrial preservative Ventron Chemical First registered: 1956 ------- —2— 2. Use Patterns and Formulations flxlecylguanid me 1X’decylguanid me I ecylguanid the acetate hydrochloride (IX H) terephthalate (ccr) i gistered Fruit and Industrial Industrial Sites: nut trees, aquatic sites preservative ornainentals, industrial aquatic sites, çxilp and paper products Predcininant Apple trees Industrial Non—clothing Use(s): biocide/ textiles preservative Formulation ttable p ,vider/ Soluble Soluble Types dust, soluble concentrate! c ncentrate/ Registered: ooncentrate/ liquid liquid liquid Method(s) of Foliar spray, Autcmatic pump, Manual pour, Application: Air blast spray, Drip Feed, pour Conventional Disting equip— padding equi ent ment drawn by tractor or truck, Bo n sprayer, Aerial spray, Autcmatic pm p, Drip Feed, Pour 3. Science Findings &a uiary: EPA has only limited data pertaining to dodecylguanidine acetate (dodine), dodecylguanidine hydrochloride (1X I), aix! dodecylguanidine terephthalate (DCr). 11 available data describing IX { and DGF are limited to sane product identity and disclosure of ingredients. There are no data available describing environmental fate, ecological effects, or toxioolc, y for !X and DGF. ------- —3— Ch nical tharacteristics (dodine, IX H, and DCT) : Dxline EXH DGT Color: White Not Available Not Available Physical Crystals, Not Available Not Available State: Slightly waxy Melting 136C Not Available Not Available int: Solubility: Soluble at 7—23% Not Available Not Available in low molecular weight alcohols at r teruperture. Soluble in acids and 0.06% soluble in water at 25C. Insoluble in most other solvents. Soluble in ethanol. Molecular ight: 287.4 263.9 Not Available &wirornental Fate (dodine): There are no available data allowing EPA to assess the environmental fate of dodine. Eoolo ical Effects (dodine): EPA does not have data describing toxicity of technical grade dodine to birds or aquatic organisms. However, data on a multiple active ingredient formulation containing 95% dodine indicate that dodine is slightly toxic to birds and highly toxic to freshwater fish. A lalx)ratory acute contact study indicates that dodine is relatively nontoxic to honey bees. Although the absence of data on technical dodine limits conclusions, available data suggest that there would be no inmediate hazard to avian species fran terrestial f d crop residues. Data show that the acute U ) 50 for avian species for a formulated product are in the range of 700 to 2000 p it. Estimation of residues to which birds will be exposed are approximately 70 — 170 *n on leaves and 3—7 w °n fruit. The estimated environnental concentration (EEX2) in aquatic systems, 15 ppb, is well below the toxic level to fish (650 — 870 ppb). Based on the inc nplete data base, terrestrial dodine fcod residues pose no apparent acute hazards to any avian or aquatic species that are Federally designated threatened or endangered. ------- —4— xicology (dodine): The available data allow EPA to adequately characterize the acute effects of dodine. The findings of acute oral W 50 of 1.46 g/kg, a one hour LCSo of 1.05—1.79 nr /l, and no deaths in test animals after acute dermal exposure to 2 g/kg indicate rr derate lethal potency (Category III). Severe irritation in tx)th washed and unwashed eyes and severe dermal irritation including erythema and edema were seen in rabbits (Category I). Based on a 21-day skin sensitization study, there is no evidence of sensitization in humans. Although many of the available studies of dodine do not satisfy EPA ’s data requirements, various effects of d dine can be identified based on existing studies. Subchronic dietary exposure of dogs caused changes in thyroid cells indicative of stimulation (No Cbserved Effects Level (NOEL) = 1.25 n 3/kg). In another study, chronic dietary exposure in the rat caused reduced weight gain in both sexes, accompanied by a comparable reduction in fcod consumption in males (NOEL = 10 nra/kg). EPA classifies this rat study as supplementary because the histopathology analysis was inadequate. In a reproductive study, dietary exposure of parent mice caused decreases in the number of pups per litter surviving until day 5 and weaning (Lowest Observed Effect Level (LOEL) = 74—89 ni /kg. The results of an es assay for mutagenicity of 5 strains of bacteria with and without metabolic activation were negative. Iblerance Reasses nent (dodine): The initial ceptable I ily Intake (ADI) and Provisional ADI (PADI) for dodine are both based on a chronic 12 nonth dog study. Treated dogs exhibited histological changes in the thyroid described as increased vascularity and changes in the shape of follicular epithelial cells fr zn squamous to cuboidal. These changes are considered to be indicative of thyroid stimulation. The No Observed Effect Level (NOEL) of 50 pp n (1.25 n* /kg) reported in the study was confirmed in the reasses nent for this standard. EPA calculated a PADI of 0.0013 /kg for dodine. This value is based on a safety factor of 1000 for interspecific and intraspecific extrapolations and the uncertainty of risk due to gaps in the chronic data base. The PADI is equivalent to a Maximum Permissible Intake (MPI) of 0.078 ma/day. 1t Theoretical Maximum Residue Concentration (ThI ) of dodine in the daily diet is 0.005117 rw /kg/day (0.307 no/day for a 60 kg person). Under these assumptions, 393.6% of the PADI is used. 4. S iiinary of Regulatory Positions and Rationales — EPA will not, at this time place dodine, EX , or DGr into Special Review because, based on available data, none of these chemicals meets or exceeds the criteria for oorkiucting a Special Review (40 CFR Part 154.7). — EPA will not require restricted use classification for end—use products containing dodine, D(] , or tx r. D dine is not acutely toxic via the inhalation or oral routes. There are data demon- strating that exposure to dodine can cause severe ocular and dermal irritation. However, labeling provisions for protective clothing will minimize acute risks from use -of products containing dodine. ------- -5- — EPA will not require specific label statements pertaining to the protection of Federally designated threatened or endangered species because the available data for dodine do not suggest that terrestrial food residues pose acute hazards to threatened or endangered species. — EPA is not proposing a label advisory statement regarding groundwater because there are, at present, no data suggesting cause for groundwater concerns. — EPA is adopting the following positions regarding dodine residues and tolerances: (1) EPA will revoke, within one year of issuance of this standard, the spinach tolerance if no use for this crop is proposed. (2) EPA will revoke or replace the zero tolerances for meat and milk. (3) EPA will delete the restriction against feeding apple p nace to livestock after registrants sut nit data den nstrating either no residues in ix iace or the need for a tolerance. — EPA is not imposing a reentry internal for dodine at this time. }bwever, EPA is requiring data needed to establish a reentry interval. — Labels of products containing dodine must bear language requiring the use of either protective clothing or closed mixing - loading systems to reduce the potential risk of skin and eye exposure. — EPA will not allow any significant new uses of dodine, D(. i, or i T until data gaps identified in this Standard are filled because the available data are insufficient for EPA to evaluate risks associated with the uses of these chemcials. — EPA will review inTnediately upon receipt of the data: 40 CFR Part 158.125 1 sidue chemistry 171—4 Nature of metaboli n in plants — dodine 40 CFR Part 158.135 lbxicology 82—1 90-day feeding — D(r 82—2 21-day dermal - dodine 83—1 Chronic toxicity, rodent and non-rodent — dodine 83—2 cogenicity, 2 species — dodine 83—3 Teratogenicity, 2 species — dodine 83—4 Reproduction, 2 generation — dodine 40 CFR Part 158.145 Ecological effects 72—3 Oyster LC 50 — dodine, DG , and LET 72—7 Aquatic residue rw nitoring — dodine, DG , and DGr ------- —6— 5. Suim ary of Major Data Gaps Dee Date Study [ dine DGH DGT Product Chemistry Product identity and ccxnposition Jan. 1988 Nov. 1987 Nov. 1987 Analysis and certification of July 1988 May 1988 May 1988 product ingredients Physical and chemical characteristics Jan. 1987 Nov. 1987 Nov. 1987 Residue Chemistry (only dodine) Metabolism July 1989 Analytical methods Jan. 1989 Storage stability July 1989 Residue in plants July 1988 July 1989 Residue in aniii al products Jan. 1989 &wirorunental Fate (ckidine and D( i) Degradation (laboratory) Apr. 1988 Feb. 1988 Photogradation Apr. 1988 Feb. 1988 Metabolism (laboratory) Oct. 1989 Aug. 1989 Mobility July 1988 Dissipation (field) Oct. 1989 — Pug. 1989 Sept 1991 Acct ulation July 1988 — May 1988 Sept 1990 July 1990 lbxicology Acute studies Apr. 1988 Feb. 1989 90 day feeding (rodent) — Sept 1988 90 day feeding (non—rodent) — Jan. 1989 Reserved 21 day derinal July 1988 May 1988 Reserved 90 day dermal — - Reserved 90 day inhalation - Reserved Chronic toxicity (rodent & non—rodent) Sept 1991 — Reserved Oral oncogenicity (rat and n ouse) Sept 1991 - Reserved Teratogenicity (2 species) Oct. 1988 — Reserved Reproduction Jan. 1990 - Reserved Mutagenicity July 1988 May 1988 Reserved General metabolism July 1989 May 1989 Reserved Wildlife and Aquatic Organisms Avian and manmalian studies Apr. 1988 — Feb. 1988 Feb. 1988 July 1989 Aquatic organisms Apr. 1988 Feb. 1988 Feb. 1988 Estuarine and marine organism July 1988 May 1988 May 1988 Aquatic monitoring July 1989 May 1989 May 1989 ------- —7— 1 e L te Study - EY dine DGH o cr I entry Foliar dissipation Sept 1989 Dermal exposure Sept 1989 6. Contact Person at EPA 3ohn Lee (PM —31) Registration Division (TS—767C) Dis i nfectants Branch 401 M Street, S.W. Washington, D.C. 20460 Tele: (703) 557—3675 DISCLAIMER; The information presented in this Pesticide Fact S et is for informational purposes only and may not be used to fulfill data requirements for pesticide registration and reregistration. ------- |