PB84-128305
Evaluation of  the  Methods Used to
Determine Potential  Health Risks Associated with
Organic Comtaminants in the Great Lakes Basin
Minnesota Univ., Minneapolis
Prepared for

Environmental  Research Lab.-Duluth, MN
Jan 84
                 U.S. DEPARTMENT OF COMMERCE
               National Technical Information Service

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                                                  Pb84-128305
                                                 EPA-600/3-34-002
                                                 January 1984
EVALUATION OF THE METHODS USED TO DETERMINE POTENTIAL
HEALTH RISKS ASSOCIATED WITH ORGANIC COMTAMINANTS IN
                THE GREAT LAKES BASIN

                          by
      Leonard M. Schuman, M.D.,  M.S.,  Professor
     Conrad P. Straub, Ph.D., Professor Emeritus
 Jack S. Mandel, Ph.D.,  M.P.H.,  Associate Professor
      Stephan Norsted, M.P.H., Research Fellow
      J. Michael Sprafka, M.S.,  Research Fellow
              Division of Epidemiology
    Division of Human Health and the Environment
               School of Public  Health
   University of Minnesota, Minneapolis, MM 55455
                  EPA Grant 806282
                 EPA Project Officer
                     W. R. Swain
            Large Lakes Research Station
                Grosse lie, MI 48138
          ENVIRONMENTAL RESEARCH LABORATORY
          OFFICE OF RESEARCH AND DEVELOPMENT
         U.S. ENVIRONMENTAL PROTECTION AGENCY
                  DULUTH,  MM 55804

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                                    TECHNICAL REPORT DATA
                             (ftegg rtad Instructions on the rertnt btfon computing)
\. REPORT f>O.
  FPA-600/3-84-Q02
                               2.
             3. RECIPIENT'S ACCESSION NO.
                 P8?
4. TITLE AND SU8TITLI
 Evaluation of  the Methods Used  to Determine  Potential
 Health Risks Associated with  Organic Contaminants in
 the Great Lakes Basin
             S. REPORT OATS
              .lanuarv 1984
             A. PERFORMING ORGANIZATION CODE
7. AUTMOR(S)
 L.M. Schuman,  C.P. Straub,  J.S.  Mandel, S.  Norsted and
 J.M. Sprafka
                                                               . PERFORMING ORGANIZATION REPORT NO.
13. PERFORMING ORGANIZATION NAME AND ADDRESS
 School of  Public Health
 University of Minnesota
 Minneapolis,  MN  55455
                                                              10. PROGRAM ELEMENTNO.
             ll.CONTRACT/GR A N TNO.

               806282
 12. SPONSORING AGENCY NAME AND AOOAESS
  Environmental Research Laboratory
  Office of Research  and Development
  U.S.  Environmental  Protection Agency
  Duluth, MN 55804
             13, TYPE OP REPORT AND PERIOD COVERED
             14. SPONSORING AGENCY CODE
                EPA/600/03
 18. SUPPLEMENTARY NOTES
 18. ABSTRACT
 These  results suggest  that "lake-bordering" populations (i.e., white  populations)
 experience higher  rates of mortality  due to stomach  and esophageal  cancers as compared
 to  "non-lake bordering" counties.  This trend is consistent when  the  potential
 confounding factor of  large urban centers is removed.
  7.
                                  KEY WQROS ANO DOCUMENT ANALYSIS
                    DESCRIPTORS
                                                 b.lOENTIFIERS/OPEN ENDED TERMS
                              COSATl Field/Croup
  J8. DISTRIBUTION STATEMENT
  RELEASE TO PUBLIC
                                                  16. SECURITY CLASS ITUl Ripofl)
                                                   UNCLASSIFIED	
                            21. NO. OF PAGES
                              41 f
M. SECURITY CLASS (T*itpogtf
  UNCLASSIFIED.
                                                                             23. PRIGS
  EPA
        ' 1220-1 (»•». 4-77)   ontvtoua ZOITIOW ID OUBOLSTB

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ATTENTION







PORTIONS OF THIS REPORT ARE NOT LEGIBLE



HOWEVER, IT IS THE BEST REPRODUCTION



AVAILABLE FROM THE COPY SENT TO NTIS.

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                      NOTICE

This document has been reviewed in accordance with
U.S. Environmental Protection Agency policy and
approved for publication.  Mention of trade names
or commercial products does not constitute endorse-
ment or recommendation for use.

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                             TABLE OF CONTENTS


List of Tables                                                       vii

List of Figures                                                       ix

Acknowledgements                                                       x

Polychlorinated Biphenyls in the Environment

     I.  Introduction                                                  1

    II.  Physical and Chemical Characteristics of PCBs                 7

   III.  Sources and Translocation of PCBs in the Environment         16

Health Effects of PCBs

     I.  Animal Models                                                20

    II.  Human Exposures to PCBs and Other Organic Compounds          29

         A.  General                                                  29

         B.  Dietary Exposures                                        32

         C.  Exposure via Breast Milk                                 35

         D.  Community Surveys                                        40

         E.  Occupational Studies                                     44

         F.  Teratogenic Research                                     46

   III.  Summary                                                      47

Analyses of Morbidity and Mortality Patterns of Populations
Living in the Eight Great Lakes States                                50

     I.  Analysis 1                                                   51

         A.  Introduction                                             51

         B.  Methods                                                  52

         C.  Results                                                  53

         D.  Discussion                                               54

    II.  Analysis 2                                                   54

         A.  Introduction                                             54

         B.  Methods                                                  55

         C.  Results            _                                     56

                                  i i i

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   III.  Analysis 3                                                   56

         A.  Introduction                                             56

         B.  Methods                                                  57

         C.  Results                                                  57

         D.  Discussion                                               59

    IV.  Analysis 4                                                   59

         A.  Introduction                                             59

         B.  Methods                                                  60

         C.  Results                                                  60

         D.  Discussion                                               62

     V.  Analysis 5                                                   62

         A.  Introduction                                             62

         B.  Methods                                                  63

         C.  Results                                                  74

             1.  Urban and Rural Analysis                             74

             2.  "Rural" Analysis                                     76

         D.  Discussion                                               79

A Pilot Study to Determine the Feasibility of an Epidemiologic
Investigation Among Commercial Fishermen of the Affects of
Polychlorinated Biphenyls on Health

     I.  Background and Rationale                                     85

    II.  Aims and Objectives                                          89

         A.  General                                                  89

   III.  Methods                                                      89

         A.  Study Protocols                                          89

         B.  Identification and Selection of a Study Population       90

         C.  Questionnaire                                            95

         D.  Protocol I Procedure                                     96

                                    IV

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     E.   Protocol II Procedures                                     98




     F.   Protocol III Procedures                                    98




     G.   Timetable for Pilot Project Initiation                    100




     H.   Validation Procedures                                     102




     I.   Tracing Procedures                                        104




     J.   Coding                                                    108




     K.   Data Analysis                                             109




IV.  Pilot Study Results                                           111




     A.   Protocol I                                                111




     B.   Protocol II                                               114




     C.   Protocol III                                              117




     D.   Discussion                                                1




 V.  Cost Analysis for Protocol Implementation                     1




     A.   Protocol I                                                1




     B.   Protocol II                                               1




     C.   Protocol III                                              1




     D.   Validation                                                1




     -E.   Tracing                                                   1




Conclusions                                                        1




Methodological Approaches for Continued Research                   1




Bibliography                                                       1




Addendum                                                           1




Appendix I  Sates- and Ratios for Thirty-five Cancer Sites




Appendix II  Study Questionnaires and Survey Instruments




Appendix III  Pilot Study and Survey Materials

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Appendix IV  Proposed Set of Coding Instructions




Appendix V  Proposed Set of Analytical Procedures




Appendix VI  Progress Reports
                                   VI

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                              List of Tables

                                                                       page

Table 1      Molecular Composition of Some Aroclors (part A)	4

Table 2      Molecular Composition, of Some Aroclors (part 3)	5

Table 3      Physicochemical Characteristics for Several PCB Compounds... 8

Table 4      Solubility of Chlorobiphenyls and Isomers in Water	10

Table 5      Vaporization Rates of Several Aroclors.	11

Table 6      Summary of the Health Effects of PCBs on Animal Models... .21-23

Table 7      Summary of the Health Effects of PCBs on Animal Models...  24

Table 8      Summary of the Health Effects of PCBs on Animal Models...  24a

Table 9      Summarization of the Toxic Effects of PCBs on Aquatic
             Animals	26-28

Table 10     Levels of Organochlorine Residues in Human Adipose
             Tissues	31

Table 11     Percent of U.S. Breast Milk Samples With Levels of
             Chlorinated Hydrocarbon Pesticides or Their Metabolites
             at £51 ppb by Geographical Region  (n = 1,436)	37

Table 12     Organochlorine Pesticide Residue Levels in Whole Milk
             Samples from Women Residing in Arkansas and
             Mississippi (n = 57)	39

Table 13     Percent of Lake Bordering and Non-Lake Bordering Counites
             Having Morbidity/Mortality Rates Discrepant by > 1
             Standard Deviation From State Means	58

Table 14     Means, Standard Deviations, and Comparisons of Mean
             Age-Adjusted Cause-Sex-Race-Specific Mortality Rates
             per 100,000 for Rural "Lake Bordering" and Rural "Non-
             Lake Bordering" Counties of the Great Lakes Basin	61

Table 15     Counties Designated to the 1st, 2nd, and 3rd Orders by
             State	64-70

Table 16     Summary Population Figures for the Great Lakes Basin by
             Order, Sex, and Race (1960)	71

Table 17     Summary Population Figures for the Great Lakes Basin by
             Order, Sex, and Race (1960)  Counties with urban centers
             containing populations > 100,000 are omitted	72

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Table 18     Cancer Sites Demonstrating INCREASING Average Mortality
             Rates With Proximity to the Great Lakes Basin	80

Table 19     Rural Analysis - Omitting Counties With Population
             Centers  >100,000
             Cancer Sites Demonstrating INCREASING Average Mortality
             Ratas With Proximity to the Great Lakes Basin...;	81

Table 20     Cancer Sites Demonstrating DECREASING Average Mortality
             Rates With Proximity to the Great Lakes Basin	82

Table 21     Rural Analysis - Omitting Counties "With Population
             Centers   100,000
             Cancer Sites Demonstrating DECREASING Average Mortality
             Rates With Proximity to the Great Lakes Basin	83

Table 22     Levels of PCBs in Canadian Fish Species	86

Table 23     Sources of Information Regarding Commercial Fishermen	91

Table 24     Number of Commercial Fishermen in the Great Lakes Basin
             by Lake and State	93

Table 25     Status  of Protocol 1 Participants	112

Table 26     Status  of Protocol II Participants	116

Table 27     Status .of Protocol III Participants		119

Table 28     Preliminary Results for Protocols I and II	122

Table 29     Historical Perspective of Commercial Fishing Records	148

Table 30     Breakdown of Study Eligibles, Respondents and Survey
             Response Rates	"...	152

Table 31     Distribution of Study Eligibles, Respondents and
             Response Rates by State	 .153

Table 32     Preliminary Results for Great Lakes Study	155
                                    VI 1 1

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                              List of Figures
                                                                        page

Figure 1          Stereochemistry of PCBs	2

Figure 2          Simulated Movement of PCBs Through the Environment	18

Figure 3          Flow Chart of Protocol I Procedures	99

Figure 4          Flow Chart of Protocol III Procedures	101

Figure 5          Protocol Implementation	103

Figure 6          Flow Chart of Validation Procedures	105

Figure 7          Flow Chart of Tracing Procedures	107

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                      Acknowledgements
The assistance of the following in the project is gratefully acknowledged:

                  Division of Epidemiology

                        Deb Englehard
                          Ann Berry
                        Joan Maronde
                         Pat Mingee
                       Irwin Pollack
                       Virginia Sykes
                    Division of Biometry

                         James Boen
                      Laurie Reinhardt

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            Polychlorinated Biphenyls in the Environment



    I.  Introduction




        Polychlorinated biphenyls (PCBs) consist of a group of com-




pounds produced by the complete or partial chlorination of a biphenyl




molecule.  These compounds belong to a family of chlorinated hydro-




carbons in which chlorine has replaced hydrogen in the molecular




structure (54 ).  The stereochemistry of a PCB molecule is demonstrated




in Figure  1.  The molecule consists of two benzene rings joined at




their apices with the potential for chlorine substitution on the




remaining ten sites on the rings.  The number of theoretically possible




isomers is 209, but far fewer have been found in the environment (54 ).




This may result from manufacturing processes producing specific molecu-




lar compositions eliminating the undesirable chlorinated species and/or




environmental metabolism and degradation.




     PCBs were discovered in 1881, but industrial applications and




commercial production were discovered and undertaken in 1929 (50 ).  The




Monsanto company was the sole producer of PCBs in the United States and




marketed its products under the trade name Aroclor.  Other PCB producers




are found in Germany, France, Italy, Japan, Russia, and Czechoslavakia




and market their products under a variety of trade names ( 54).




     Aroclor PCB mixtures are identified by a four digit number with




the first tiwo digits representing the type of molecule (12 - chlorinated.




biphenyl) and the latter two the average percentage of chlorine by




weight  in the mixture.  For example, Aroclor 1248 represents a PCB mix-




ture with approximately 48 percent chlorine by weight.  Tables 1 and 2




depict  the molecular composition of several Aroclor products.  A compar-




ison of  the reported values for Aroclors 1242 and 1254 in Tables  1  and  2

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                                    Figure 1
    Sterozhenistrv of FCBs
    Numbering system for the biphenyl molecule
 4'
                      2'
    exarole
        Cl
ci
Cl
      Cl
    3, 4, 4', 5' - tetrachlorobiphenyl

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demonstrates variability in the percentages of chlorinated species.




This may be attributable to differences in quantitative analysis and/or




the products themselves.  In addition, the variability in total and




isomeric PCB percentages may be compounded when evaluating the analyti-




cal results of PCBs extracted from environmental matrices.  In 1970,




when these widespread industrial compounds were recognized as a potential




problem, Monsanto restricted the domestic sales of Aroclors to uses in




closed electrical and hydraulic systems which effectively cut in half




the annual U.S. sales of PCBs.  Veith reported data obtained from




initial monitoring of PCBs in waters around the Green Bay, Wisconsin




region indicating that PCB levels decreased sharply in 1971 suggesting




that the self-imposed limitations on PCB production by Monsanto had




immediate effects (112).




     The production of PCBs by Monsanto stoppei in August, 1977 and




the sales of inventory stock ceased two months later.  The complete ban




on PCBs came into effect on July 1, 1979 with ;he enactment of the




Toxic Substances Control Act (PL-94-46) (  1 ).  However, over the years,




approximately 1.25 billion pounds of PCBs have been purchased by U.S.




industries.  Of that amount,it is estimated that 55 million pounds of




PCBs have been destroyed by incineration or environmental degradation,




290 million pounds have been disposed in landfills and are assumed to




have retained their toxic properties, and 150 million pounds are "free"




in  the environment (i.e., air, water, sediments, and animal tissues).




The remaining 755 million pounds purchased by U.S. industries are




currently in use (29).  In addition, several foreign companies still




produce PCBs without any restrictions on their use and U.S. companies




are free to Import these products.  Estimates by the Environmental



                                   3

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                         Table 1

         Molecular Composition of Some Aroclors
A.  Chlorobiphenyl composition
% Aroclor product
    C  H Cl
     129

    C  H Cl
     128  2

    C  H Cl
     127  3

    C  H Cl
     126  k

    C  H Cl
     1 2 5  S

    C  H Cl
     1 2 W  6

    C  H Cl
     1 2 S  7

    C  H Cl
     122  6

    C  HC1
     12    9
1242
3
13
28
30
22
4
-
-
_
1248 1254
-
2 _
18
40 11
36 49
4 34
6
-
_ _
1260
-
-
-
-
12
38
41
8
1
    Hutzincer, 0. et al..  The Chemistry of PCBs.   CRC Press,
    Cleveland (1974).  p. 23

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                          Table 2


           Molecular Composition of Some Aroclors






B.  Chlorobiphenyl composition    1221   1016   1242    1254


    C  H
     12 10

    C  H Cl
     12 9

    C  H Cl
     128  2

    C  H Cl
     127  3

    C  H Cl
     126  t

    C  H Cl
     125  S

    C  H Cl
     1 2 H  6

    C  H Cl
     123  7

    C  H Cl
     122  8
11
51
32
4
2
<0.5
ND*
ND
ND
<0.1
1
20
57
21
1
<0.1
ND
ND
<0.1
1
16
49
25
8
1
<0.1
ND
<0.1
<0.1
0.5
1
21
48
23
6
ND
    * ND - Not Detected ( 0.01%)
    Hutzinger, 0. et al.  The Chemistry of PC3s.  CPC Press
    Cleveland  (1974).  p. 23.

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Protection Agency indicate chat the United States has been importing




500,000 pounds of PCBs per year as of 1976 and an unknown, amount .of




PCB-containing products ( 1 ).




     In order to evaluate the potential environmental effects of PCBs




sampling surveys must produce quantitative information regarding PCB




concentrations in various environmental strata (i.e., air, water,




sediments, and food products).   These data, characterizing PCB behavior




in the environment, may help to predict transportation routes and




identify high risk exposure groups in human populations.  The purpose




of this section is to review the physical and chemical characteristics




of PCBs and the mechanisms regarding introduction and transport through




the environment.  In addition,   a review of the potential health effects




of PCBs on animal models and humans will be presented.

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    II.  Physical and Chemical Characteristics of PCBs




         The physical and chemical properties of PCB isomers and mix-




tures vary considerably when these materials are tested in the laboratory.




Chlorine substitution markedly affects the three physicochemical char-




acteristics important in the evaluation of the potential environmental




impact and these include solubility, vaporization rates, and the




capacity to biodegrade.  The solubility and volatility of a particular




PGB compound represent potential mechanisms for introduction into the




environment and will determine the ease of distribution throughout




ecosystems.  Biodegradation represents a natural mechanism for the




potential redistribution or removal of these materials from the environ-




ment.




     The solubility of PCBs in water is low; however, reported values




vary according to the isomeric structure, PCB type, and the composition




of the aqueous test solutions.  Dexter and Paulou analyzed several PCB




isomers and found that their solubilities were five times greater when




tested in sea water as compared to distilled water.  This suggests that




the potential for environmental contamination may be greater in ocean




and estuary environments than fresh water environments (28 ).




     In general it can be stated that as chlorine content increases,




the solubility and volatility decreases.  Table 3  depicts the




differences associated with chlorine content, as evidenced by PCB




type, and several physicochemical parameters (i.e., water solubility,




vapor pressure, theoretical half-life) (29 ).  These findings indi-




cate that the association between increases in chlorine content and




predicted decreases in solubility and volatility is not consistent




with respect to Aroclor 1254.  In addition, the values predicted for




                                   7

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                           Table 3

  Physicochemical Characteristics for Several PCB Compounds
                                                 Theoretical Half-life
               Solubility/    Vapor Pressure/      for Vaporization
  PCB Type        mg/1        	mm Hg	    From 1 Meter  H2 0 Column

    1242           0.24          4.06 x lO"**          5.96 hours

    1248        5.4 x 10~2       4.94 x 10~          58.3 minutes

    1254        1.2 x 10~2       7.71 x lo""          1.2 minutes

    1260        2.7 x 10~2       4.05 x 10~5         28.8 minutes
Durfee, 3. L. et al.  PCBs In the United States Industrial Use
arid Environmental Distributions.  Versar, Inc.  EPA  560/6-76-005
(1976).  p. 43.
                              8

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the vaporization half-life of these Aroclors from a one meter column of




water demonstrate inconsistency regarding PCS type (i.e.,  chlorine




content).   The solubility variations between individual PCB isomers




are evident from data reported by Durfee in Table  4 .   Increasing




chlorine substitution decreases the water solubility.   The vaporization




rates of several.Aroclors are shown in Table 5 demonstrating that as




chlorine content increases the vaporization rate decreases.




     In the environment, however, PCBs are subject to many influences




which may alter standard laboratory classifications. As chlorine




content increases in PCB molecules the solubility and volatility




decrease while the sorption capacity increases (15 ).   The particular




matrix to which these molecules sorb will determine their availability




in the environment.  For example, the strength of adsorption in sedi-




ments and soils demonstrates a positive correlation with the concentra-




tions of humic acid, ilite clay, and Del Monte sand in  that order (95 )•




In addition, Hague and his associates report that vapor losses from a




sand surface are significantly greater than losses observed from an




organic soil ( 43 ).  Pierce and his associates concluded that suspended




humic particulates may be an important transportation mechanism through




the water column ( 96 ).  Hence, similar PCB molecules  sorbed to




different environmental substrates may have different solubility and




volatility characteristics and may move through the environment at




different rates.  Evaluation of these variables ±s essential in order




to assess the potential environmental impact of PCBs.




     The degradation of PCBs in the environment by two  major routes




has been described in the literature.  These include microbial activity




and photochemical reactions.  The microbial degradation of PCBs involves




                                  9

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                           Table 4

     Solubility of Chlorobiphenyls and Isomers in Water
Compound

Monochlorobiphenyls
    2-
    2-
    4-

Dichlorobiphenyls
    2, 4 -
    2, 2' -
    2, 4' -
    4, 4' -

Trichlorobiphenyls
    2, 4, 4' -
    21, 3, 4

Tetrachlorobiphenyls
2,
2,
2,
2,
2,
2,
3,
o
2
2
O
3
3
3
, 5,
, 3,
/ 3,
, 4,
, 4,
, 4'
• 4,
5'
3'
5'
4'
4'
, 5
41
-
-
-
-
-
-
-
                      '  -
Pentachlorobiphenyls
    2, 2', 3, 4, 5' -
    2, 2', 4, 5, 5' -

Hexachlorobiohenyl
    2, 2', 4", 4', 5,

Octachlorobiphenyl
    •>  51  T  -31  4
    •£ I • £ I Jf J / *> f
Decachlorobiphenyl
    4, .4' - Dichlorobiphenyl

+ Tween 80 0.1%
-i- Tween 80 1%
+• Humic acid extract
                             i_
                                                   Solubility rng/1
                                                          5.9
                                                          3.5
                                                          1.19
                                                          1.40
                                                          1.50
                                                          1.88
                                                          0.08
                                                          0.085
                                                          0.078
                                                          0.046
                                                          0.034
                                                          0.170
                                                          0.068
                                                          0.058
                                                          0.041
                                                          0.175
                                                          0.022
                                                          0.031
0.0088


0.0070
                                                          5.9
                                                        >10.0
                                                          0.07
Durfee, R. L. et al.  PCBs in the United States Industrial Use
and Environmental Distributions.  Versar, Inc. EPA 560/6-76-005
(1976) ?. 47.
                              10

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                           Table 5

           Vaporization Rates of Several Aroclors
(Surface area:
12.28 cm2)
    1221

    1232

    1242

    1248

    1254

    1262

    1260

    1270 (Deca)
Wt. Loss,
    g
 0.5125

 0.2572

 0.0995

 0.00448

 0.0156

 0.0039

 0.0026

 0.0015
Exposure at
  100°C, hr.
    24

    24

    24

    24

    24

    24

    24

    24
Vaporization rate
	g/cm2/hr	
    0.00174

    0.000874

    0.00038

    0.000152

    0.000053

    0.000013

    0.000009

    0.000005
Durfee, R. L. et al.   PCBs in the United States Industrial Use and
Environmental Distribution.   Versar,  Inc.  EPA 560/6-76-005
(1976)  p. 45.
                               11

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hydroxylation and oxidation reactions which convert-aromatic  compounds




into ring-fission, substrates (25 ).   These reactions may follow dif-




ferent metabolic routes in aerobic environments as compared  to  anaerobic




environments which may ultimately affect the rates of microbial degrada-




tion and the availability of these contaminants to higher organisms.




     Rogoff reported that soil pseudomonads appear very active  in the




degradation of PCB compounds.  He demonstrated that cleavage of the




ring is preceded by hydroxylation necessary to provide two hydroxyl




groups on the aromatic ring.  These usually occur in the ortho  position




and the ring is cleaved across a bond adjacent to one of the carbon




atoms which bears a group (102).




     Furukawa.and his associates studied the effects of chlorine sub-




stitution on the degradability of PCBs using species of Alcaligenes and




Actinobacter.  They concluded that degradation decreased as chlorine




substitution increased.  PCB isomers containing more than four  chlorine




atoms were less susceptible to degradation.  Preferential ring  fission




of the PCB molecules occurred with non-chlorinated or lesser chlorinated




rings.  They also noted that positional variations in the isomers




affected the rate of degradation.  Those PCBs containing all the chlorine




atoms on a single ring were generally degraded faster than when the




same number of chlorine atoms were substituted on both rings (37 ).




     Ahmend and Focht report data which indicate that some species of




Achromobacter are capable of oxidizing PCB isomers with two to  five




chlorine atoms.  However, the bacteria are unable to dehalogenate any




of these chlorinated biphenyls as noted by the absence of chloride in all




the supernatants.  They conclude that increasing the chlorine substitu-




tion renders the biphenyl molecule more resistant to microbial  attack




                                  12

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( 3 ).   In subsequent studies by these investigators they succeeded in




isolating two species of Achromobacter from sewage effluent using bi-




phenyl  and p-chlorobiphenyl as sole carbon sources ( 2 ).




     Sayler and his associates report that both PCB-degrading bacteria




and PCBs were found in higher concentrations in estuarine waters and




sediments compared with marine samples.  Their data indicated a greater




correlation between PCS concentrations and numbers of PCB-degrading




bacteria in areas of urbanization as opposed to areas distant from land.




Their results suggest that the assessment of existing PCB-degrading




bacteria in environmental samples may be used as indicators for potential




PCS contamination.  They caution, however, that the number of PCB-




degrading microorganisms in a given sample cannot be directly correlated




with the concentration of PCBs in that sample (106).




     It may be assumed from these studies that certain microbial species




have the capacity to degrade these complex molecules but, in the natural




environment, will preferentially attack simpler energy forms.  If situ-




ations occur in the environment where simple carbon sources are exhausted,




microbial species could, theoretically, develop pathways- to metabolize




more complex molecules like PCBs.  However, the extent of microbial PCB




degradation by natural microbial populations remains undetermined.




     Photodecomposition is considered to be another potential mechanism




for PCB degradation because of its similarities with chlorinated hydro-




carbon pesticides  (.i.e., DDT) and the mechanisms associated with their




degradation  (.104).  However, Durf ee _e_t -al_., conclude that the probability




of UV disassociation of chlorobiphenyls appears significantly less than for




the chlorinated pesticides ( 29).




     Hutzinger and his associates investigated the photochemical behavior




                                 13

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of 2, 2', 4, 4', 6, 6* hexachlorobiphenyl in organic" solvents.   Results




Indicate a loss of chlorine, rearrangement, condensation, and the for-




mation of new compounds.  They caution that photochemical production of




new compounds may lead to further complications  in  residue analyses




with the possible appearance of chlorobiphenyls in environmental samples




that are not found in commercial samples.  Additional studies by Hutzinger




et al., indicated that higher chlorinated biphenyls appear to degrade




faster than those with lower chlorine content when exposed to a radiation




source ( 53,54 )•




     These results may have limited applicability to an environmental




situation since these experiments were conducted in organic solvent




systems (i.e., hexane) rather than water.  Additional experimental




strategies such as irradiation of chlorobiphenyls in the gas and solid




phases as well as the relationship of positional isomerism and photo-




chemical activity must be examined as potential mechanisms of PCB




degradation in the environment.




     The environmental behavior of PCBs is also influenced by their




solubility in lipids and fats -resulting in a tendency to bioaccumulate




in organisms progressively higher in the food chain (118).  As a con-




sequence PCB routes through the environment may be subject to specific




metabolic mechanisms which, in turn, are subject to the solubility




differences of PCBs in the lipids of these organisms (88 ).




     Biological concentration is a process whereby an organism demon-




strates a greater concentration of a particular chemical factor as




compared to the surrounding substrate or various fo.od sources.  This




phenomenon is influenced by such factors as the amounts of .chemical




present in the diet or surrounding environment, the chemical and




                                  14

-------
physical forms of the contaminant,  the feeding and behavior traits of




the animal species in question, the degree of assimilation through




cellular barriers, and the extent of retention in the tissue (41 ).




     Nisbet and Sarofim report that the more highly chlorinated isomers




of PCBs are retained more efficiently; probably the result of metabolism




and differential excretion ( 88 ).   Their preliminary conclusions were




that the more highly chlorinated isomers are not significantly differ-




entiated as they pass through the food chain up to fish and birds.




They concluded that differential metabolism is the primary mechanism in-




the environmental differentiation of isomers.  Risebrough and his associ-




ates believe that current data suggest that the amounts and kinds of




lipids may affect the capacity for retention of PCBs, modifying trophic




accumulation predicted by the classical food chain concentration theory




(100 )•



     Several strategies have been proposed to study the bioconcentration




phenomenon and these include self-containing ecosystems, dynamic circu-




lating systems, and an equilibrium-type ecosystem.  Self-containing




ecosystems are limited because of maintenance considerations and expense




but offer a totally simulated but contained ecosystem.  Dynamic circulating




systems are those in which the contaminant is introduced into the system




at a prescribed rate while equilibrium ecosystems are those in which the




contaminant is present in the environment and the organisms are intro-




duced  into the system ( 95 ).  However, any model used to evaluate




bioconcentration mechanisms has to incorporate and adjust for variations




in eating habits, lipid content, age, sex, and size, all of which affect




PCS levels in higher organisms.




                                 15

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    III.  Sources and Translocatlon of PCgs in the Environment
          Environmental contamination with PCBs may be the result of
both point and non-point sources.  Point sources include vaporization
or leaking from transistors and capacitors, municipal- and industrial-
effluent discharges, leachates from landfills and dumps, and the incin-
eration practices of manufacturers and municipalities.  Non-point
sources include land application of municipal sludges and chemicals
followed by runoff, and the atmosphere ( 85 ).
     Recent evidence suggests that a major source of PCBs may be the
degassing of sanitary landfills and industrial dumps.  Investigators
have determined a difference of three orders of magnitude between
ambient air and vent gas PCS concentrations ( 46 ).  Gaffney^demonstrated
that chlorobiphenyls can be produced in the effluents of municipal
wastewater treatment plants which practice chlorination and receive.
industrial wastes containing biphenyls (i.e., textile industries) ( 38).
The experimental technique, however, was one of "super-chlorination"
so the results may not be applicable to municipalities conducting
normal treatment operations.  Lawrence and Tosine report that localized
areas of heavy industry may be responsible for trace organic contaminants
in sewerage systems.  These contaminants become associated with the
suspended solid content of the sewage and are incorporated in the
sludges following primary and secondary treatment (75 ).  Land applica-
tion, of these sludges..may increase the potential for environmental
contamination following runoff.
     Maugh has investigated natural sources of chlorobiphenyls and
hypothesizes that a common metabolite of plants and animals, dichloro-
benzophenone, may be degraded to a dichlorobiphenyl compound by photo-
                                 16

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lysis ( 81).  However, reaction rates are very slow and the exact




chemical pathways are as yet undetermined.  In addition, most PCBs in




the environment have been identified as penta- and hexachlorobiphenyls




which suggests that PCBs come from artificial (anthropogenic) rather




than natural sources ( 88 )•  Figure 2 demonstrates the potential




mechanisms by which PCBs are introduced and transported throughout




various ecosystems.




     It is generally accepted that the atmosphere is the major route of




entry and subsequent transport through the environment as evidenced by




its ubiquitousness ( 88 ).   Once in the atmosphere PCBs are distributed




between the gaseous and particulate phases and are transported through-




out the environment via the prevailing air masses.  Most authors agree




that atmospheric transportation occurs more frequently in the vapor-




aerosol state rather than the particulate state ( 16,45,46,85 ).




     Harvey and Steinhauer hypothesize that during the atmospheric




lifetime of chlorinated hydrocarbons the vapor-aerosol and particulate




states will equilibrate and exchange many times depending on the condi-




tions  ( 45  ).  Both dry and wet deposition are potential mechanisms for




loading into aquatic and terrestial ecosystems.




     Water  transport is considered to be more localized because of the




low solubility of PCBs.  PCS discharges into the aqueous environment




will, for the most part, be associated with particulate matter and will




settle out  as sludges or become adsorbed to the bottom muds.  However,




uptake by zooplankton and fish and subsequent excretion may provide a




transportation route through the aquatic ecosystem via fecal pellets.




Elder and Fowler proposed that PCBs are carried to the sediment by




rapidly sinking particles  (i.e., fecal pellets) and these make a sig-




                                  17

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                                                       Figure  2
                                  SIMULATED MOVKMBNT OF 1'Cllo THROUGH TUB ENVIRONMENT
Mnvf»nu:nt In
 00
        Monu far. taring
          (spills, leaks, breaks)

        Capacitor processing
          nnd disposal

        Municipal and industrial
          effluents

        Incineration  practices
          of industries and
          municipalities
                                    Water
                                                 HovcmonC Out
Volatility  (aeration)
Rainout/fallout
                                  Air
                                                           Land  and
                                                           Landfills
                                                   Leachate
                                                                          Rainout/fallout
Photo-
decorepo-
                                          sition
                                                                           Volatility (vent gases)
                                                   Runoff
                                                                 Dredging
                                                        Adsorption
                                                        Desorption
                                                                            Aquatic
                                                                            Sediments
                                      Uptake/
                                      Bioconcentration
Aquatic Biota
                                   Clearance  (fecal  pellets)
                                                                                     Biodegradation
                                                                                        Higher Organisms

-------
nifleant contribution to the vertical transport of these substances in




aquatic environments.  They reported data demonstrating PCB concentra-




tions in feces (dry weight) ranging from 3.5 to 21 times higher than




that in organisms which formed the feces ( 31  ).




     In conjunction with the fecal transport hypothesis consideration




must be given to the metabolic actions by aquatic organisms (i.e. ,  fish)




on PCB contaminants.  Metabolic functions followed by excretion may




alter PCB structure which, in turn, may influence the toxicity or  avail-




ability of these materials to other organisms in the aquatic ecosystems.




In addition, these metabolized products may alter PCB structure compli-




cating quantitation.
                                  19

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                        Health Effects of PCBs-




I.   Animal Models




      Several excellent reports have been written which review the




health effects of PCBs  in experimental animal models.  These include the




IARC Monograph on the Evaluation of  the  Carcinogenic Risk of Chemicals




to Humans  (  55 ), The Health  Effects of  PCBs with Particular Emphasis




on Human.High Risk Groups C 21 ), and the Final Report of the Subcom-




mittee on  the Health Effects  of Polychlorinated Biphenyls and Poly-




brominated Biphenyls (33 ).   These  reports are briefly summarized in




Tables 6-8 respectively.




     In general, the principal organs affected by long-term .exposure to




commercial mixtures of  PCBs in animals are the gastro-intestinal  tract,




the liver, and the lymphatic  system  (21).  Reported pathological effects




include carcinogenic and tumorigenic potential, immunosuppression, embryo-




toxicity,  and reproductive dysfunctions  (see Tables  6-8  ).  In  addition,




PCBs have  several sublethal effects  such as microsomal  enzyme induction,




clastogenic  activity, and modified  cell  development.




     Thompson  e_t al_. conclude that  Aroclor 1254  is a wide spectrum




inducer of mutagenic activity based  on their studies involving  the




effects of PCBs  on rat-liver  homogenate  (59 preparations).  In  addition,




they reported  that the  hydroxylations of the number 2  and 4 chlorines




proceed via  the  areiie oxide intermediate pathway  which has been impli-




cated  in  carcinogenesis and mutagenesis  0-11) •




     Hargraves and Allen investigated the  in vitro binding of tetra-




chlorobiphenyl (.TCB) to rat liver microsomes and  reported a protein(s)




in  the induced system capable of binding a TCB metabolite.  They  con-




clude  that their results support the concept of  a metabolic activation




                                  2Q

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                                   TABLE  6
        Summary of the  Health  Effects of PCBs  on Animal  Models
1)  Carcinogenesis

    Mice:
         hepatocellular carcinoma
         hepatoma
    Rats:
         adenofibrosis of  the liver
         cholangiofibrosis
         hepatocellular carcinoma
         neoplastic liver  nodule
2)  Toxic Effects

    Rats:
         liver hypertrophy, marked fatty  infiltration and degeneration
              of parenchymal  cells

    Chickens:
         subcutaneous edema,  ascites,  hydropericardium
       •  loss of fat
         marked involution of the thymus
         atrophy of the spleen
         increased liver weight
         congestion, mild necrosis and marked infiltrations of the liver
         widespread hemorrhage and focal  necrosis in the kidney

    Rabbits (skin application):

         weight loss
         hyperkeratosis of the skin
         liver-cell atrophy
         kidney cell degeneration
         atrophy of the thymus
         lymphopenia

    Rabbits (oral):

         liver hypertrophy
         atrophy of the uterus

    Monkeys:

         acne
         swelling of upper eyelids
         loss of eye lashes
         alopecia
         subcutaneous edema
         gastritis
         ulceration
         hypoproteinemia
         anemia                         21

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                               Table 6  (Continued)
3)   Immune Effects

    Guinea Pigs:
         lymphopenia, thymic,  and splenic atrophy
         decrease in number of circulating lymphocytes
    Rabbits:
         lymphopenia, thymic,  and splenic atrophy
         decrease in number of circulating lymphocytes

    Monkeys:

         lymphopenia, thymic,  and splenic atrophy
         decrease in number of circulating lymphocytes

    Duck:
         increased hepatitis  susceptibility
4)  Endocrine Effects

    Eats:

         estrogenic effect

    Nonhuman primates:

         increased level of urinary ketosteroid-s
         prolonged menstrual cycles with increased bleeding



5)  Eabryotoxicity, Teratogenicity, and Reproductive Effects

    Rats:
         increased mortality of the offspring
         reduced mating performance
         reduced litter size
         decreased survival of the offspring
         decreased maternal weight gain
         decreased fetal weight
         learning disability

    Chickens:

         reduced hatchability
         abnormalities in the embryo

    Rabbits:
         fetotoxic abortions and stillbirths
         increased maternal deaths

                                         22

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                          Table 6  (Continued)
Monkeys:

    facial acne and edema
    swelling of the eyelids
    loss  of facial hair
    hyperpigmentation of the skin
    gastritis
    keritinization of the hair follicles
    early abortions
    small offspring
reference:  IARC Monographs on the Evaluation of  the  Carcinogenic
            Risk of Cehmicals to Humans.   Volume  18,  (1978).
                                    23

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                                     TABLE 7

             Summary of the Health Effects of PCBs on_Animal Models


a)  Health Effects of PCB:  Animal Studies

    1.  Effects on Liver and Spleen:

        weight increase, fatty degeneration,  and necrosis of the liver in
          birds and a variety of mammals
        increased activities of some drug metabolizing enzymes
        reduced vitamin A storage in the liver
        altered lipid metabolism
        depletion of lymphatic nodules in the spleen in pheasants

    2.  Carcinogenesis and Tumorigenesis:

        hepatic adenofibrosis in rats
        biliary epithelial hyperplasia in rats
        induction of nodular hyperplasias in rats
        hepatocellular carcinomas in mice

    3.  Effects in Adrenal Gland:

        morphological alteration in the zona fasciculata of adrenal gland in rats
        increased levels of cor.ticosterone in rats

    4.  Effects on the Reproductive System:

        decreased mating indices in rats
        number of young delivered, number surviving to weaning, both decreased
        increased number of stillborns
        decreased egg production in chickens
        decreased egg shell thickness
        decreased reproductive capacities in pheasants

    5.  Effects in Chromosomes:

        chromosomal aberrations in Ring Dove Embryos

    6.  Immunosuppression:

        reduction in lymphoid tissue, and the presence of amyloid or amyloid-like
           material in the liver of chicks
        lymphopenia in rabbits
        decreased number of antibody-forming cells after stimulation of lymphoid
           system in guinea pigs.

Reference:  "The Health Effects of PCBs with Particular Emphasis on Human High
            Risk Groups," Edward J. Calabrese, Reviews on Environmental Health
            Vol. 2(4), 1978.

                                       24

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                              TABLE 8

      Summary of the Health Effects of PCBs on Animal Models


Animal Toxicology

1)  Reproduction depression:

    mink
    rhesus monkey
    rata

2)  Hepatic porphyria:

    chicken
    rabbit
    Japanese quail
    rats

3)  Liver damage and tumors including hepatocellular carcinoma:

    rat
    mouse
    primate

4)  Bone marrow depression:

    primate

5)  Gastric mucosa damage:

    rat
    dog
    primate

6)  Atrophy of the thymus:

    rabbit

7)  Fluid accumulation:

    chickens
    finches
    primates
Reference: Final Report of the Subcommittee on the Health Effects of Poly-
           chlorinated Biphenyls and Polybrominated Biphenyls, Dept. of
           HEW, Washington, D.C., July, 1976.
                                    24 a

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of microsomes resulting from PCB insult ( 44 }.   Subsequent studies by




Stadnicki je£ al. evaluated the potential clastog«nic effects of TCB and




its phenolic and arene oxide intermediates.  They report that 2, 2', 5,




5', TCB-3-4 epoxide was more potent in causing DNA single-strand breaks




than either 2, 2', 5, 5', TCB or a mixture of 3-hydroxy or 4-hydroxy-2,




2', 5, 5' TCB.  They conclude that PCB epoxide intermediates may poten-




tiate carcinogenesis ( 108).




     Ohnishi and Noda evaluated the effects of Kanechlor 400 on dissoci-




ated conjunctival cells in vitro and reported an increase in the mitotic




times of epithelial cells exposed to PCBs.  Greater cellular damage was




observed as PCB concentrations were increased,  infrastructure changes




of epithelial cells included an increase in the number of vacuoles,




lysosome-like particles, myelin-like figures, and a dilute rough endo-




plasmic reticulum filled with secretion in the cytoplasm ( 89 ).




     Hoopingarner and his associates investigated the effects of PCBs




on Chinese hamster cells and primary human lymphocyte cells.  In general,




there was a steady drop in cell numbers as the percentage of chlorine in




the PCB mixture decreased for both types of cells.  They reported no




apparent effects on chromosomal integrity as measured by cytological




evidence ( 49).




     In addition to the health effects of PCBs on experimental animal




models, several investigators have evaluated the toxicity of PCBs on




fish and other aquatic animals.  A summary of these results appears




in Table  9  ,  Of particular interest is the "bioconcentration factor"




which has been reported by several researchers.  Essentially, this




"bioconcentration factor" is determined by measuring the contaminant




concentration in water or food sources and  the resulting contaminant




                                  25

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                                 TABLE  9

     Summarization of the Toxic Effects of PCBs on Aquatic Animals
Animal Model:
    Fathead Minnow

Experimental Methodology:
    Flow-through bioassays were conducted to determine both acute and chronic
     effects of the larvae and adults, as well as the bioconcentration of the
     mixture of FCBs in the fish.

Experimental Results and Conclusions:
    30-day LC-50 for newly hatched larvae was 4.7 mg/1 for Aroclor 1248
    30-day LC-50 for newly hatched larvae was 3.3 mg/1 for Aroclor 1260
    reproduction occurred at or below 3 mg/1 1248 and at or below 2.1 mg/1 1260_
    bioconcentration factor in adult females at 25°C was approximately 1.2 X lO
     for Aroclor 1248 and 2.7 X 105 for Aroclor 1260
    females accumulated about twice as much as males
    the chromatograms of 1248 and 1260 residue were essentially identical to
     .the standard after a 200 day exposure.  Subtle changes occurred in the
     first and second peaks (Defoe, 1978)
Animal Model:
    Fathead Minnow

Experimental Methodology:
    Continuous-flow bioassays were conducted to determine safe levels of
     Aroclor 1242, 1248, and 1254 for the fathead minnow

Experimental Results and Conclusions:
    96-hour LC-50 for newly hatched larvae was 7.7 mg/1 for Aroclor 1254
    96-hour LC-50 for newly hatched larvae was 15.0 mg/1 for Aroclor 1242
    96-hour LC-50 for 3 month old fatheads was 300.0 mg/1 for Aroclor 1242
    reproduction occurred at or below 1.8 mg/1 1254 and at or below 5.4 mg/1
     1242
    males accumulated more 1254 and 1242 than females after a period of
     eight months  (Nebeker et.al., 1974)
Animal Model:
    Flagfish

Experimental Methodology:
    continuous-flow bioassays were conducted to determine safe levels of
     Aroclor 1248 for the flagfish (Jordanella floridae)

Experimental Results and Conclusions:
    no survival at or above 5.1 mg/1 and did not grow well above 2,2 mg/1
    (Nebeker, 1974)
                                          26

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                                  -TABLE 9 (Continued)

       Model:
    Daphnia

Experimental Methodology:
    Continuous-flow and static bioassays were conducted at 18°C with survival
     and reproduction as measures of relative toxicity of Aroclors 1221, 1232,
     1242, 1254, 1260, 1262, and 1268
    Three PCB-mixture bioassays were also conducted

Experimental Results and Conclusions:
    3 week LC-50 for Daphnia under static conditions was 25 mg/1 for Aroclor 1248
    3 week LC-50 for Daphnia under continuous-flow conditions was 1.3 mg/1 for
     Aroclor 1254 (Nebeker and Puglisi, 1974}


Animal Model:
    Gaaaaarus

Experimental Methodology:
    Continuous-flow and static bioassays were conducted at 18°C with survival
     and reproduction as measures of relative toxicity of Aroclors 1221, 1332,
     1242, 1254, 1260, 1262, and 1268
    Three PCB-mixture bioassays were also conducted

Experimental Results and Conclusions:
    96-hour LC-50 for Garmnarus under continuous-flow conditions was 73 mg/1 for
     Aroclor 1242
    96-hour LC-50 for Gammams under continuous-flow conditions was 20 mg/1 for
     Aroclor 1248
    60-day survival percentage was 52% at 8.7 mg/1 1242 and 53% at 5.1 mg/1 1248
    (Nebeker and Puglisi, 1974)


Aniaal Model:
    Tanytarsus

Experimental Methodology:
    Continuous-flow and static bioassays were conducted at 18°C with survival
     and reproduction as measures of relative uoxicity of Aroclors 1221, 1232,
     1242, 1248, 1254, 1260, 1262, and 1268.
    Three PCB-mixture bioassays were also conducted


Experimental Results and Conclusions:
    emergence did not occur above 5.1 mg/1 1248 or 3.5 mg/1 1254
    3 week LC-50 for Tanytarsus larvae under continuous-flow conditions was
     0.65 mg/1 for Aroclor 1254
    3 week LC-50 for Tanytarsus pupae under continuous-flow conditions was
     0.45 mg/1 for Aroclor 1254 (Nebeker and Puglisi, 1974)
                                          27

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                                   TABLE 9 (Continued)
Animal Model:
    Coho Salmon

Experimental Methodology:
    Yearling coho salmon were fed PCB diets and mixed PCB-mirex diets to
     determine the effects on hepatosomatic indices, liver lipids, carcass
     lipids, and bioaccumulation

Experimental Results and Conclusions:
    hepatosomatic indices were increased in salmon fed PCB diets and a mixed
     PCB-mirex diet and were decreased in salmon fed Mirex diets compared to
     controls
    liver lipids in PCB-fed fish were significantly increased and carcass
     lipids significantly decreased
    carcass PCB levels in Coho fed high PCB diet (500 mg/kg) were 3.2 fold
     higher than Coho fed the low PCB diet (50 mg/kg)
    carcass PCB levels in Coho fed a mixed PCB-mirex diet were significantly
     higher than fish fed low PCB diet (Leatherland ejt.al., 1979)


Animal Model:
    Ciscoe

Experimental Methodology:
    Continuous-flow bioassays were  conducted to measure the acute toxicity
     of arsenic and PCBs, singly and in combination for ciscoe fry (Coregonus)

Experimental Results and Conclusions:
    96-hour LC-50 for ciscoe fry was>10 mg/1 for a mixture of 1248, 1254 and 1260
    96-hour LC-50 for ciscoe fry was 3,5 mg/1 for the PCB-arsenic mixture
    the difference in PCB concentration between male and female C.hoyi was highly
     significant (joale ciscoe -* 2.3 mg/g)
                 (female ciscoe-»1.2 mg/g)
    (Passlno and Kramer, 1980)


Animal Model:
    Br.ook Trout

Experimental Methodology:
    Continuous-flow bioassays were conducted to determine the effects of 1 mg/1
     and lower concentrations of Aroclor 1254 on the life cycle of the brook trout

Experimental Results and Conclusions:
    no adverse effects observed on survival and growth during 71 weeks of expo-
     sure or on their progeny exposed to 90 days of Aroclor 1254
    body levels reached an apparent steady state bioconcentration factor 1 X
    (Snarski and Puglisi, 1976)
                                          28

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concentration in fish tissues.  The concentration of PCBs in the




tissues of experimental fish models (i.e., fathead minnow, brook trout)




is approximately 1.0 X 10  greater than that in the water (26).  Jarvinen




and his associates evaluated the bioconcentration factor of other




chlorinated hydrocarbons (i.e., endrin, DDT) and reported similar results




to those reported for PCBs (58).  This suggests the potential for bio-




accumulation in natural aquatic ecosystems given sources of PCS contam-




ination.  It is interesting to note the conclusions from a study by




Weininger which indicate that direct uptake (.i.e., from water to fish




tissue) accounts for only 2 - 3% of the total PCB accumulation by adult




lake trout in Lake Michigan.  He suggests that food, primarily the




alewife, is the major contributing source of PCB body burden in lake




trout  (115).  In addition, he concludes that a large proportion (>50%)




of the PCB burden in Lake Michigan lake trout has been cycled through




the sediments suggesting that potential'public health problems could




persist for years.  The human health implications of the behavior of




PCBs in the aquatic environment, resulting from a demonstrated ability




to bioaccumulate in fish, have not been adequately evaluated.











II.  Human Exposures to PCBs and Other Organic Compounds




     A.  General




         The results of surveys and experiments which have examined




human  exposures to selected organic chemicals are presented below.




Polychlorinated biphenyls  (PCBs) serve as the principal model for




organic compounds throughout  this review.




     Information on the level of PCBs in  the general population of the




                                  29

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United States has been released by the Human Monitoring Survey (HMS).




Founded in 1967,  the purpose of this organization has been to examine




the exposure of the general population to pesticides.  Residues from




these compounds are measured in adipose tissue samples acquired from




post-mortem examinations and therapeutic surgeries.   Results from the




HMS reported by Yobs in 1972 (117) state that 31.1% of 637 samples




acquired contained one or more parts per million (ppm) of PCBs.  In a




comparison of gas chromatography with thin-layer chromatography by




the HMS (98 ), both methods provided similar results indicating that




41-45% of the U.S. population have PCS levels of 1.0 ppm or more with




isomers from Aroclors 1254, 1260, 1262, and 1268.  A summary of "other"




organochlorine residue levels for the years 1970-1974 inclusive are




listed in Table  10 (  74 ).  The authors stated that residues of alpha-




benzene hexachloride, llndane, and mlrex were detected in adipose




tissues infrequently  (<2.05%).  Residues of delta-BHC, aldrin, hepta-




chlor, and  endrin were not detected in the samples.




     The following conclusions may be drawn from the HMS data:




                 a.  Residues of various organochlorines are present




                      in quantifiable amounts in the general population




                     of the United States.




                 b.  Between 30-50% of the general population have




                     adipose tissues with >1.0 ppm PCBs.  The most




                     common isomers encountered are penta-, hexa, and




                     heptachlorobiphenyl compounds (73 ).




                 c.  HMS data for 1973 and 1974 indicated an increased




                      percentage of tissues with trace levels of PCBs




                     while the limit of detection (1.0 ppm) remained




                     constant (73),




                                  30

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                                               TABLE 10



                     Levels of Organochlorine Residues  in  Human Adipose Tissues
Compound
Beta-Benzene
llexachlor Ide
Total DDT
Equivalent
Dieldcin
lleptachlor
Epoxide
Oxychlordane
Survey
Years
1970-
1974
1970-
1974
1970-
1974
1970-
1974
1970-
1974
Average
Sample
Size
1307
1387
1387
1387
1302
Average Percent of
Samples With
Detectable Residues
97.7
99.9
90.4
95.0
96.4
Average Geo-
metric Mean
in ppm.
0.28
6.72
0.18
0.086
0.12
Comments on Data
Set Averaged
A trend was demonstrated
toward a reduction in
concentration over time
but not in the frequency
of occurrence
Same as Beta-Benzene
llexachloride
The geometric means and
percent of samples
which were positive were,
relatively consistent
across the survey year
Same as Oieldrin
Same as Dieldrin
Source:  Kutz et.al.(1977)

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     The sources of exposure to PCBs for the general population include




foods,  ambient air, ambient waters,  contaminated soils,  and occupational




exposures.




     B. Dietary Exposures




        Estimates  of PCB consumption from market-basket samples



indicate an average adult  ingestion of  5-10Ug per day ( 21).   Variabil-




ity in PCB consumption may result from special diets or accidental con-




tamination of food products.  Vegetarians may experience a lower exposure




to PCBs as they limit fresh fish consumption.  Accidental contaminations




of human food stuffs are exemplified by the leakage of PCBs into




"Shredded Wheat." from packaging materials ( 66 ), the introduction of




contaminated poultry feed  into Holly Farms livestock ( 66 ), and the




"Yusho"" (oil disease) incident ( 71).




     The Yusho epidemic represents one of the largest case studies of




acute toxic effects of PCBs in humans.  The following description is




a summary of the author's  findings.  In Fukuoka-ken, Japan a local




rice oil-producing company developed a leak in a heat-exchanger.  The




oil was contaminated with 2,000-3,000 ppm Kanechlor 400 (48% chlorinated




biphenyl).  The average amount of PCBs ingested by subsequent patients




has been  estimated at 2 grams.  The approximate minimum dose was 0.5




grams.  The mean blood level among Yusho patients was 0.7 parts per




billion Cppb).  The predominant initial symptoms experienced by 136 of




the Yusho patients were increased eye discharge, swelling of upper




eyelids,  acniform eruptions  (chloracne), follicular accentuation, and




increased skin pigmentation.  Common, late-appearing symptoms included




those noted above  as well as weakness, sweating of palms, and brown





                                  32

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pigmentation of  the nails.   Children born  to patients and wives of




patients  had a high incidence of grayish,  dark=-brown skin, gingiva, and




nails.  Increased  eye discharge, low birth weight, and infantile jaundice




•were also noted.   Of the  13  pregnancies reported, two were stillborn.




At a three-year  follow-up half of  the  patients had clinically improved,




over 10%  had worsened, with  the remaining  patients demonstrating no




change.   The conclusion which may  be drawn from  the follow-up period is




that clinical recovery is not common.




     A summary of  the clinical findings is presented in  the reference by




Kuratsune (70).   His group  found  that severe "Yusho" patients experienced




an increase in leucocytes, total lipids, triglycerides,  serum lipoprotein




plc< ratio, Cu,  serum globulin oC,  and alkaline  phosphatase.  A decrease




was found in red blood cells, hemoglobin,  and Fe.  The cases with charac-




teristic  "Yusho" acne had higher levels of cholesterol and stearic  and  oleic




acids  than those with normal acne.  The pathological responses observed




in the Yusho epidemic could  be explained by  the  presence of chlorinated




dibenzofurans  in the rice oil.   Chlorinated  dibenzofurans are a contam-




inant  of  PCBs and  are known  to be  highly toxic.   Blood PCS levels as




high as in Yusho patients but without  adverse health effects




have been reported in occupationally  exposed workers  (33 ).  Therefore,




conclusions cannot be drawn  between the physiologic responses attributable




to either of  the two compounds.




      Current  foods which  may provide  high  PCB exposure to the general




population are  fish and human breast milk  (33 ). Results from the  Food




and  Drug  Administration  (FDA) Total Diet Study  (1971-1975)  indicate




that PCBs are  no longer detected in food categories other.than in meat-




fish-poultry  composites.  The presence of  PCBs  in this category  is





                                  33

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attributed to fish.   It is estimated that 93% of the U.S.  population




consumes fish.  The average fish eater consumes 15 pounds  of fish per




year, which provides an approximate PCB consumption of 5-10 micrograms/




day.  The freshwater fish species most commonly consumed are trout,




bass, catfish, and pike.  A large fraction of fish consumed in the




U.S. diet are unclassified breaded products (e.g., fish sticks).  PCB




measurements have typically not been initiated in this fraction (24  ).




Further difficulties in describing PCB exposure via fish consumption




are due to:  (1) the inconsistent monitoring of PCB levels in fish;  (2)




most surveys having been based on small sample sizes; (3)  PCB analyses




usually being done on whole fish and not restricted to the edible por-




tions; (A) fish being  acquired for consumption without passing through




commercial sectors where monitoring may be undertaken; (5) variations




in PCB concentrations in individual fish by species, age,  and geograph-




ical area.




     A .study by Humphrey ( 52 ) and others reported on the serum PCB




levels of Lake Michigan sport fish eaters.  The authors found these




sport fishermen consume an average of 24-25 pounds of fish/person/year.




A highly  significant correlation was found between the amount of Lake




Michigan  fish consumed and blood PCB concentrations.  Those fishermen




who consumed less than 6 pounds/year had a mean blood value of 0.02 ppm.




Those who ate more than one meal per week, or 24 pounds per year, had




an average blood value of 0.073 ppm.  This latter group had an estimated




average intake of 1.7ydg/kg body weight/day of PCBs.  The FDA recom-




mends that PCB intake not exceed 1 Mg/kg body weight/day.   The authors




found that blood PCB levels did not decrease significantly when fish




consumption was eliminated for up to nine months, nor did the levels





                                  34

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alter appreciably from year  to year.  A correlation between blood PCS




levels and a pathological condition was not  found in the high exposure




group.  The authors conclude that while Lake Michigan fish contribute




to blood PCB levels in humans, the dose received has not produc'ed a




detectable health effect.




     C.   Exposure via Human Breast Milk




         The other food,  noted above,  which may provide high




exposure to PCBs is human breast milk.  From 1974 to 1977  Kodama




and  Ota  ( 65 )  studied blood PCB  levels in volunteer women and.  their




newborns at a hospital in Alchi Perfecture,  Japan.  The following




samples were acquired by the authors:   (1) maternal blood at 8  and 4




months prepartum; at delivery, and 1, 3,  5,  and  7 months postpartum;




(2)  mother's milk at 1, 3,  5, and 7 months postpartum;  (3) cord blood




at delivery;  (4) newborn infant blood at 3 months,  1 year, 2 years,




and  3 years after birth.   The results of the study  indicate that maternal




blood PCB  levels  increase  with gestation and thereafter decrease to




general population  levels  by 5 months postpartum.   Maternal blood at




delivery has a  significantly higher PCB level  than  cord blood.  When




considering cord blood as  newborn blood,  PCB blood  levels in breast-fed




infants  increased with ingestion  of human milk.  At 3 months postpartum




the  blood  levels  in the  infants exceeded that  of their mothers. The




peak blood  PCB  level was reached  at one year of  age and decreased there-




after.   Those  infants who  were bottle-fed had  consistently low  levels




over the same  time  period.   The authors conclude that  the quantity of




PCBs passed from mother  to  child  is greater  in lactation than through




the  placenta.




     In  the "National Study of Chlorinated Hydrocarbon  Insecticide





                                  35

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Residues in Human Milk, U.S.A." (105 ) breast milk samples from 1436




women were obtained.  Ihe percent of samples positive for dieldrin,




heptachlor, and oxychlordane by geographical region are presented in




Table  11 .  Eighty percent of all samples collected had detectable




levels of dieldrin.  Oxychlordane, a metabolite of- chlordane, was




detectable in 74% of the samples.  Heptachlor was found in less than




2% of the samples, yet its metabolite heptachlor epoxide was found in




63% of the samples.  The authors note that after adjusting for fat




content in the samples, the mean residue levels for the three compounds




in Table 11 were 164.2 ppb for dieldrin, 91.4 ppb for heptachlor epoxide,




and 95.8 ppb for oxychlordane.  It was quite common for a single subject




to have relatively high values for more than one compound.  The trend




for higher mean values in the southeastern U.S. was attributed to more




extensive use of home pesticides and termite control.




     Studies which support the above findings of organic contamination




of human breast milk were conducted in Michigan, Arkansas, and Mississip-




pi.  The Michigan study (116) was conducted in 1977 and 1978.  Breast




milk samples from 1,057 nursing mothers were analyzed for PCS residues.




PCB levels ranged from trace amounts to 5.1 ppm, with all 1,057 samples




positive  Cfat weight basis).  The mean PCS level was 1.5 ppm.  PCB




levels of 1-2 ppm were found in 49.5% of the samples, 2-3 ppm in 17.4%




of those  sampled, and 6.14% of the women had greater than 3 ppm.  Approx-




imately one-half of the women had PCB levels equal to or greater than




the present FDA tolerance limit for cow's milk.  If an infant were breast-




fed by a woman having the mean PCB level (1.5 ppm) in this sample, the




child would have an estimated body burden of 0.89 ppm of PCBs.  The




infant's PCB body burden would increase steadily with the consumption





                                 36

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                                                 TABLE 11

      Percent of U.S.  Breast Milk Samples With Levels  of Chlorinated Hydrocarbon  Insecticides  or
                      Their Metabolites at >51 ppb by Geographical Region (N = 1,436)
                                             Geographical  Region
Compound
Dieldrin
Oxychlordane
Heptachlor
Epoxide
Northeast
%
55
54

43
Southeast
%
84
72

56
Midwest
%
74
57

57
Southwest
%
76
54

25
Northwest
7,
69
52

24
Total U.S.
%
73
58

42
Source:  Savage et.  al.  (1981)

-------
of breast milk for the entire period of breast-feeding.   The authors




stress that the women in this study may not be representative of all




Michigan women as they were not randomly selected.   They further state




that the PCB levels found in several counties in the western part of




the lower peninsula as well as in three counties of the upper penin-




sula exceeded 2 ppm.  It is speculated that these levels may reflect a




higher dietary intake of PCB-contaminated fish from the Great Lakes.




     Fifty-seven women residing in Arkansas and Mississippi contributed




whole milk samples for organochlorine pesticide residue analysis.  The




results from this survey are presented in Table 12 (  74 )..  The percent




of samples positive range from 14.1 (trans-nonachlor) to 100.0 (total




DDT equivalent; p, p'-DDT; p, p'-DDE).  The arithmetic mean concentra-




tion in ppm ranged from 0.01 for many pesticides to 0.34 for total DDT




equivalent.  The discrepancy in values of the percentages positive be-




tween Tables  11 and  12 for the respective compounds may be due to




differences in analytical techniques, sample size,  properties of the




geographical areas surveyed, and a multitude of demographic variables.




Yet, both tables are consistent in the finding that significant propor-




tions of the women surveyed have contaminated breast milk.




     A study of Dutch mothers and their infants (  30 ) provides evidence




which challenges a number of the conclusions drawn above.  Pregnant




women were divided into four groups:  CD those not on a slimming diet




who breast fed; (2) those not on a slimming diet who bottle-fed; (3)




those on a slimming diet who breast fed; (4) those on a slimming diet




who bottle-fed.  Maternal blood samples were acquired as early in




pregnancy as possible and, at parturition, from the umbilical cord and




placenta.  Post-natal blood samples at 10 days, 6 weeks ,  and  3 months





                                  38

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                                        TABLE 12

              Organochlorine Pesticide Residue Levels in Whole Milk Samples
                 from Women Residing in Arkansas and Mississippi  (n=57)
Pesticide
Total DDT equivalent*
pf p1 - DDT
p, p' - DDE
ft - BHC
Dieldrin
Heptachlor expoxide
Oxychlordane
trans-nonachlor
Percent of Samples
Positive for Pesticide
100.0
100.0
100.0
36.8
28.1
25.1
45.6
14.1
Arithmetic Mean of
Concentration in ppm.
0.34
0.09
0.22
<0.01
<0,01
<0.01
<0.01
<0.01
Source:   Kutz et.al.  (1977)

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were acquired from the infant and mother.  Milk samples were taken daily




fXQJn breast-feeders.  Data are not presented on organochlorine levels in




human milk.  The results of this study indicate that higher organochlo-




rine concentrations are not found in the blood of breast than of bottle-




fed infanta.  Further, there are no differences between the organochlo-




rine concentrations in the blood of breast than of bottle feeding mothers.




Between 12 and 21% of the daily intake of dieldrin by mothers was




eliminated in their milk and ingested by the infants.  The corresponding




range for total DDT was 36 to 61%.  The ranges were based upon the




infants' consumption level during the first 3 months postpartum.  It




is interesting to note that the blood concentrations in slimming and




non-slimming mothers were very similar except for those for dieldrin.




As would be expected from the lipid solubility of organic compounds,




dieldrin concentrations were higher in the slimming diet mothers.




     Several investigators who have reviewed the health risks of organic




compounds in human breast milk have concluded that no obvious pathology




has resulted from breast feedings by the non-occupationally or acutely




exposed mother (13, 101, 62).  In an article on PCBs in human breast




milk (82) the author states that unless a woman has a definite history




of PCS exposure, she should be encouraged to breast-feed her infant.




Kendrick  (62) presents a more cautious appeal for the weighing of




potential risks against known benefits in breast feeding.




     D.  Community Surveys




         Testimony to the ubiquitous nature of organic compounds has




been provided by their prevalence in the adipose tissue of the




general population and human breast milk.  Community based studies




have been initiated to assess the general health effects of these pol-






                                  40

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lutants.  The residents  of  Triana, Alabama were studied in 1979 since




they were known to consume fish contaminated with DDT and PCBs (69  ).




Serum PCB levels were determined for 458 subjects.  The geometric  mean




serum level was 17.2y^g/L.  A positive association was found between




PCB levels and age after controlling for sex, local fish consumption,




obesity, serum cholesterol level, and alcohol consumption.  PCB levels




were also positively associated with gamma-glutamyl transpeptidase




level, serum cholesterol level, and blood pressure.  This latter posi-




tive association was independent of age, sex, body mass index, and social




class.  The rate for borderline and definite hypertension was 30%  higher




for the study group than would be expected from national rates for a




demogra;3hically similar population.  PCB exposure was associated with




changes in liver function tests.  Breast-fed children did not have sig-




nificar.:ly higher PCB levels than bottle-fed children, although, a




positive trend was demonstrated between breast-feeding and DDT.  The




authors conclude that the association found between PCB levels and




blood pressure, liver function, and cholesterol concentrations warrant




further investigation  since the PCB levels in this population overlap




 those found in other communities.




     The effects posed by DDT exposure on the health of the Triana




residents are discussed in a second paper  (68 )•  The national geometric




mean total DDT level in serum was 1.5 ng/ml; the respective geometric




mean for the 499 Triana residents was 76.2 ng/ml.  The source of human




exposure was contaminated local fish.  From 1947 until 1971, a DDT




plant 10 km from Triana deposited several thousand.tons of DDT waste




into a local tributary.




     An average of 86.7% of the total DDT detected was of the metabolite





                                  41

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DDE.  Acute health effects from DDT exposure were not found in the




predominantly black (86.9%)  study population.  Positive associations




with DDT levels were found with serura triglycerides,  alcohol consumption,




cigarette smoking, and liver enzyme induction.   The authors state that




their most striking finding is an increase in serum DDT levels with age




without reaching a steady-state level.  Age was the single most power-




ful predictor of DDT level (log transformation).  The rate of increase




in DDT levels in women did not change with hormonal status.  The authors




further emphasize that the increase in average serum DDT levels in the




elderly suggest age-related changes in absorption, excretion, or storage




between serum and adipose tissues.




     In 1976, the sewage sludge used by Bloomington, Indiana residents




for garden fertilizer was found to contain a mean PCB concentration of




479.1 ppm (34 ).  The PCBs had been discharged into the city sewerage




system by a local electrical manufacturing firm.  To evaluate possible




health effects from this exposure, three study groups were selected by




the Monroe County Board of Health and the CDC:   (1) occupationally




exposed workers,  (2) the workers' families, and (3) the residents who




had utilized the  sewage sludge in their garden.  The mean serum PCB




level was highest for the worders  (71.7 ppb), second highest for the




workers' families Q3.6 ppb), third highest in community residents not




utilizing sludge  in their gardens  (23.8 ppb), and lowest to. those res-




idents using the  contaminated sludge  (17.6 ppb).  The sample sizes for




each of these groups are 18, 19, 29, and 91 respectively.  Correlations




were not found between serum PCB levels and the number of years of




contaminated  sludge utilization, the  total number of pounds used, or the




length of the interval since last use.  The authors did find that plasma





                                  42

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triglyceride concentrations and serum gamma-glutamyl transpeptidase




increased significantly with increases in serum PCB levels.  These




associations were found to be independent of age.




     One of the most recent community studies evaluating the effects




of numerous toxic wastes on human health was conducted in the Love




Canal area (57  ).  When cancer rates in the Love Canal residents were




compared to data from the New York Cancer Registry, no evidence was




found for an increased risk in the disposal area.  A higher rate of




respiratory cancer was noted, but appeared to be part of a high rate




for the entire city of Niagara Falls.  The authors acknowledge that the




study design could not assess the influence of such confounding variables




as socioeconomic status, smoking, air pollution, and population migra-




tion.




     The community studies reviewed above have provided evidence that




organic compounds have been found in residential areas.  The source of




the contamination ha s often been identified as industrial wastes.  While




PCB levels have been documented in human serum, the above studies have




not demonstrated significant pathological responses.  A paucity of




studies have been performed on individuals occupationally exposed to




PCBs.  It is hypothesized that these groups experience a greater




exposure potential than the general population, and would therefore




more readily manifest any possible toxic effects.  One of the earlier




studies examined the level of PCB residues in the plasma and hair of




refuse workers (42 )•  Detectable plasma PCB levels were found in 81%




(32/37) of the refuse burners and only 11% (6/54) of the control popu.-




lation.  Scalp hair was found to have no value in estimating PCB




body burdens.  The increased plasma PCB levels in the refuse workers





                                  43

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were hypothesized to be due to refuse incineration.




     A subpopulation of Michigan residents were exposed to polybrominated




biphenyls (PBBs) in 1973 by an accidental contamination of animal feed




supplement (  19 ).  Several of the residents subsequently complained of




memory loss.  To test the hypothesis that PBB exposure may affect memory,




twenty-five chemical workers who manufactured PBBs were given learning




and memory tests.  PBBs were found in adipose tissue samples of the




workers, yet the mean scores on all memory tests were normal.  The




memory dysfunction of the Michigan residents was attributed to a psycho-




logical dysfunction and not to PBB body burden.




      E.  Occupational Studies




         Workers who have been exposed  to PCBs while manufacturing




capacitors have shown the following health effects:    (1)  14%



of 243 employees demonstrated a reduced forced vital capacity.  The mean




number of years employed in this group was 15 (114 ),  (2) for those who




had been employed for more than 10 years, the most common symptoms re-




ported involved dermatologic and CNS dysfunctions (34  ), (3) the latter




group of workers had a functional capacity of the cytochrome P-450 system




different from the non-exposed workers.  Overall, the  investigators were




impressed by  the paucity of abnormalities found in the physical examina-




tions (  34 ) •




     In an occupational retrospective cohort study of  PCB-exposed workers,




both the "all cause" and cancer mortality rates were lower than expected




(18 ).  The corresponding values were 163 observed total deaths versus




182.4 expected total deaths and 39 observed cancer deaths versus 43.8




expected cancer deaths.  Elevated rectal and liver cancer mortalities




were noted, but were not statistically significant.  An increase in




                                  44

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cirrhosis of the liver was found in one of the plants examined.  Mor-




tality due to rectal cancer and cirrhosis of the liver increased with




an increase in latency, but these trends were difficult to evaluate due




to the small number of reported deaths.  The increase in liver cancer




is noteworthy  since it agrees  with  the findings  of animal  dietary models.




     The two studies referenced below have examined the risk of hydro-




carbon-exposed workers fathering children who subsequently develop




malignancies.  Positive results were found in a Quebec study of 386




children who died of malignancies prior to 5 years of age.  A significant




excess (relative odds of 2:1)  of fathers were found to work in hydrocarbon-




related occupations  (32 ).  Negative results were found in a review of




the Finnish Cancer Registry (  40 ) •




     The dennatologic  lesions  found in  the Yusho incident have been




reported in occupational groups .  The  dermal sores have  been known




 to last  for  months  after  removal  of the workers  from  the occupational




 exposure.   Systemic effects  including  nausea,  lassitude,  anorexia,




 digestive  disturbances, impotence,  and hematuria have also been




 reported  (21).




     The occupational  studies  listed above have  found workers  to be




exposed to various  organic compounds and at  risk of experiencing a wide




range of symptoms.   A  number of methodologic problems may  be found in




these studies.  A major problem in many occupational  studies is adequate




documentation of the amount of exposure to a given worker  via  inhalation,




ingestion, and absorption. Secondly,  many of  the  industries provide




exposure to  other compounds which may  act in a synergistic, antagonistic,




or additive  manner  to  the  chemical  under study.  The  healthy-worker-






                                   45

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effect and small sample size may have minimized adverse trends.  In




conclusion, the occupational studies performed to date do not allow a




definitive statement as to the health risks of long-term low-level




exposure to organic compounds.




     F.  Teratogenic Research




         The unborn child is at risk of experiencing  the toxic effects




of organic compounds.  Many organic chemicals have documented mutagenic




and teratogenic effects.  The placental transfer of compounds decreases




with increased  molecular weight, increasing electrical charge, and




decreasing lipid solubility.  The susceptibility of the fetus



to teratogenic effects depends upon:  (1) the properties of the agent




administered; (2) the time of administration of the agent, with the




most critical period being that of organ differentiation;  (3) the size




of the dose received; (4) the number of doses given;  (5) the route of




administration to the mother; and (6) other factors,  such as the general




health of the mother ( 72 ).  The lack of certain liver microsomal




enzyme systems may predispose the infant to the toxic effects of sub-




stances which are not oxidized and excreted as rapidly as normal.  For




example, a fetus is more susceptible to the effects of alcohols and




phenols if the glucuronidation system is impaired ( 21 ).  Conditions




associated with an incomplete or improper glucuronide conjugation system




are Gilbert's syndrome and the Crigler and Najjar syndrome.




     A two-year study of mothers with children having central-nervous-




system defects was conducted examining the percent of mothers known to




have had exposure to organic solvents ( 47 ).  The results of the study




indicated  that significantly more "case-mothers" than "control-mothers"




                                  46

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had been exposed to organic solvents during the first  trimester of




pregnancy.




     Reviews on the risks of pregnancy posed by environmental contam-




inants have been provided by a number of sources  ( 79,72,33,35,21).




The discussions by these authors on the teratogenic properties of




biphehyls center on the findings of the animal studies and the "Yusho"




incident presented elsewhere in this review.









III.   Summary




      Environmental studies have shown PCBs to be extremely stable




compounds in the environment.  Their lipophilic properties allow bio-




accumulation in aquatic foodchains with man, or more accurately the




nursing infant, at the top of the trophic scale.  Testimony to the




ubiquitous nature of PCBs is found in the results  of the Human Monitoring




Survey.  A significant proportion of the U.S. general  population sampled




have detectable amounts of PCBs in their adipose  tissues.  Exposure




routes for the general population include ambient air, ambient waters,




contaminated soils, occupational sources, and diet.  The  latter two




categories may provide the most concentrated exposures.   Workers in




PCB-manufacturing industries have experienced dermatological, central-




nervous-system, and diffuse symptomatologies.  The study  designs which




have provided these results have not evaluated the long-term, low-level




toxic effects of PCBs.




     Dietary studies conclude that the two food items  which may provide




high concentrations of PCBs are fish and human breast  milk.  The physio-




logical consequences of ingesting high quantities of PCBs are best





                                  47

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exemplified by the "Yusho" incident.   Their patients demonstrated acni-




form lesions, skin pigmentation, and increased eye discharge.  Those




women exposed during pregnancy had "small-for-date" children with "cola"-




colored skin and gingiva.  Their infants were noted to have a high prev-




alence of neonatal jaundice and increased ocular discharge ( 71 ).  It




must be noted that the rice oil was also contaminated with polychlorinated




dibenzofurans, which are known to have toxic properties.




     The primary source of PCBs in the U.S. diet is fish acquired from




contaminated areas.  The study by Humphrey and others ( 52 ) has docu-




mented that Michigan sport fish consumers eat approximately 10 pounds




more per person per year than the average U.S. citizen.  The findings also




demonstrated a positive correlation between serum PCB levels and the




amount of fresh fish consumed.  It may be hypothesized that the PCB




concentrations in human breast milk increase with the total body burden




of the nursing mother.  Therefore, a mother who consumes large quantities




of contaminated fish may present a greater exposure potential to the




breast-fed infant.




     The articles reviewed indicate that consumers of fish from contam-




inated waters and infants breast-fed by those consumers warrant further




study.  The physiological consequences of long-term, low-level exposure




to PCBs in these populations are currently unknown.  Therefore the efforts




of the Great Lakes project have focused on data gathering and quantitative




analysis to determine whehter contact with contaminants in the Great




Lakes has been detrimental to local populations.  Initial data evaluations




were conducted on State Vital Statistics records.  As these documents




do not indicate relative exposure potentials, a pilot study was conducted




                                  48

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to identify fish consumers.  Summaries of the data evaluations and




pilot project are presented in the following sections.
                                   49

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             Analyses of Morbidity and Mortality Patterns of
           Populations Living in the Eight Great Lakes States

     Five quantitative analyses have been performed by the Great Lakes

Project Staff.  The purpose of these data evaluations has been to compare,

by geographical area,specific disease and death rates in the Great Lakes

Basin.  The morbidity and mortality parameters examined were those likely

to reflect human exposure to organic compounds.  Reviews were based on

cancer death rates, congenital anomaly rates, and fetal, neonatal, and

infant death rates.  Vital Statistics data were not available on these

indices for all Great Lakes states.  Pennsylvania and New York have col-

lated their records in a manner which is not readily accessible.

     The geographical unit which served as a basis for comparison was

the county.  The counties within each state were  separated  into three

"Orders."  Order 1 counties were those which contain shoreline on one or

more Great Lakes.  Order 2 consists of counties which are adjacent to

Order 1 counties.  All other counties in the eight Great Lakes states

were considered as Order 3.  Comparisons of mortality and morbidity rates

were made between Order 1 counties and the respective state mean rates,

and between Order 1 counties and those of Order 2 and 3.  The contrasting

of rates by geographical proximity to the Great Lakes was based on the

hypothesis of a "graded exposure potential."  That is, populations in

proximity to contaminated areas of the Great Lakes are more likely to be

exposed to contaminants than populations residing at greater distances.


     In summary,  the results  of  these  studies demonstrate a  trend toward

increased  esophageal,  stomach and "other  gastro-intestinal"  cancer

mortality  in Order 1 counties when compared  to respective state mean

rates.   It  may  be  hypothesized that the detrimental health effects

                                   50

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of exposure to organic contaminants in  the Great Lakes may be masked by




pollutants associated with urbanization  in cities adjacent to the lakes.




When counties with large urban centers were removed from the above




analysis, a greater percentage of Order  1 counties, continued to experience




an excess of esophageal, stomach,and bronchus,  trachea and lung combined, as




well as all-cancer mortality over the respective state mean rates.*





     The comparisons between Order L and Order  2 mean county cancer mor-




tality rates demonstrated excesses in white males and females for the site of




esophagus (31% and 26%, respectively).   White males also demonstrated excesses




for the sites of breast (46%) and thyroid gland (42%).  A 68% excess in mor-




tality due to cancer of the endocrine organs was found in white females. The




county comparisons between Orders 1 and  3 found an excess of esophagal cancer




for Order 1 white males (44%) and females (32%).  The respective values for




stomach cancer mortality excesses were  35% and  24%.





     In addition, there were no  striking discrepancies between the fetal




 death rate, the neonatal death rate, and the percent of live births with




congenital anomalies, among "lake-bordering" and "non-lake bordering" counties.






     For each of the five analyses an introduction, description of methods,




summary of results, and discussion section is presented below.







I.  Analysis 1.




    A.  Introduction





        The first step in attempting to  ascertain an adverse human health




effect from contamination of the Great  Lakes required the utilization of









* Results are for white males only.





                                      51

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existing morbidity/mortality data sets acquired from state and federal




agencies.  County vital statistics regarding fetal, neonatal, and infant




death rates and congenital anomaly rates were obtained for the states of




Minnesota, Wisconsin, Illinois,  Indiana, Michigan, and Ohio for every




fifth year from 1950 to 1975 and for the year 1977.  County data for




aite, race, sex, and age-adjusted cancer mortality rates were acquired




from the National Cancer Institute's publication "U.S. Cancer Mortality:




1950-1969." ( 80)




     The hypothesis for this study was that populations residing in areas




immediately adjacent to the Great Lakes.experienced a greater exposure




potential to lake contaminants than populations residing in areas more




distant from the lakes.  This hypothesis was based on the following assump-




tions:




     1.  Most"lake bordering"communities have increased industrial




         activity compared to most non-lake bordering communities.




     2.  Individuals living in these "lake bordering" communities were




         more likely to be occupationally exposed to those pollutants




         being discharged into the air and lakes.




     3.  Close proximity to the Lakes would lead to greater exposure




         via ambient air, soils, and water.




     4.  Fishermen living in these lake-adjacent communities were more




         likely  to fish these lakes and may have had higher exposures




         due  to  consumption of their catch.




The  analytic  evaluations performed on the above mentioned data sets have




incorporated  the concept of a graded exposure potential.




     B.  Methods




           The initial  evaluation of the above data sets was performed in





                                     52

-------
the following manner:




     1.  Counties within .each of the Great Lakes states were designated




         as either bordering the Great Lakes (hereafter termed "lake




         bordering") or not bordering the Great Lakes (hereafter termed




         "non-lake bordering").




     2.  Within each state, a mean rate for "lake bordering" counties




         was calculated for each of the mortality and morbidity parameters




         listed above.  For example, the mean infant mortality rate for




         "lake bordering" counties in Minnesota was calculated by summing




         the infant mortality rates for each of the Minnesota "lake bor-




         dering" counties and dividing this- sum by the number of "lake




         bordering" counties in Minnesota.




     3.  Correspondingly, a mean rate for "non-lake bordering" counties




         was claculated for each mortality/morbidity index within each




         state.




     4.  For each of the health parameters of interest, a mean state rate




         was calculated by summing the respective rates for all counties




         within the state and dividing this sum by the -total number of




         state counties.  The standard error of each state mean was calcu-




         lated.




     A description of the data sources, methods of analysis, and results of




this phase of the Great Lakes Project may be found in the October 16, 1978-




June 15, 1979 Progress Report to the Environmental Protection Agency.  A




copy of this report, is included as Appendix VI.




     C.   Results




          An excess of esophageal, stomach, and "other gastro-intestinal"




cancer mortality was  noted in "lake bordering" counties.  However, tests





                                     53

-------
of statistical significance were not calculated for observed differences.




The comparisons of infant, neonatal, and fetal death rates for the six




states noted above were inconclusive as they were not adjusted for maternal




age nor stratified by sex or race.




     D.   Discussion




          Many of the "lake bordering" counties have large urban centers.




The excess esophageal, stomach, and "other gastro-intestinal" cancer mor-




tality in these areas may be due to factors intrinsic to urbanization




rather than potential exposure to toxic substances in the lakes.  The




possible confounding influence of urbanization is addressed in a later




analysis.




II.   Analysis 2.




     A..   Introduction




          The amount of organic compounds found in industrial and waste-




water treatment plant effluents are monitored by state governments.  A




summary of these records are found in the "Inventory of Major Municipal




and Industrial Point Source Dischargers in the Great Lakes Basin." (56)




Knowing the levels of contaminants being released into set geographical




areas provides an approximation to the exposure potential of the respective




communities.  It was hypothesized that those counties with point-source




dischargers of hazardous materials in compliance with state effluent




requirements may have different cancer rates than those counties whose




dischargers fail to comply with state requirements.  The following




analysis was initiated to determine if correlations exist between the




recorded levels of organic compounds in industrial and wastewater treat-




ment plant effluents and  the cancer mortality rates for the respective




county.





                                     54

-------
     B.  Methods




          For each of the eight Great Lakes states the five respective




counties with the highest cancer mortality rates in 1950-1969 for either




esophageal, stomach, lung, and all neoplasms were designated as list num-




ber I.  The communities listed in the inventory above were allocated to




the following four categories in list number II:




     1.  communities with at least one industry measuring organic com-




         pounds (i.e., phenols) prior to discharge and complying with




         state effluent requriements, and in which the wastewater




         treatment plants complied with state effluent requirements on




         the basis of phosphorus (P), biological oxygen demand (BOD),




         and suspended solids (SS).




     2.  communities with at least one industry measuring organic com-




         pounds (i.e., phenols) prior to discharge and failing to comply




         with state effluent requirements, and in which a wastewater




         treatment plant failed to comply with state effluent require-




         ments (on the basis of P, BOD, and SS).




     3._  communities with at least one industry measuring organic com-




         pounds (i.e., phenols) prior to discharge and failing to comply




         with state effluent requirements, and in which the wastewater




         treatment plants complied with state effluent requirements (on




         the basis of P, BOD, and SS) .




     4.  communities with at least one industry measuring organic com-




         pounds (i.e., phenols) prior to discharge and complied with




         state effluent requirements, and in which a wastewater treatment




         plant failed to comply with state effluent requirements (on the




         basis of P, BOD, and SS).






                                     55

-------
     5.  The counties of high cancer mortality in list I were matched
         geographically to the four categories of dischargers in list II.
         For example, county X has one of the five highest rates of esoph-
         ageal cancer in Ohio.  List II was then examined to see if county
         X had a discharger.  If yes, then the name of the county and the
         category in which the discharger appeared was noted in a third
         list.
     6.  Counts of the number of counties represented in each of the four
         categories of dischargers were performed.
     C.  Results
          The results indicated that counties with non-compliant communi-
ties experienced higher stomach and kidney cancer mortality rates in white
males  and high "nose, auxiliary sinus, etc." cancer mortality rates in non-
white males.  Counties with compliant communities had elevated "nose,
auxiliary sinus, etc." cancer mortality rates in white males and females.
A full account of the analytical methods and results are presented in the
"Preliminary Report of Epidemiological and Environmental Data" dated April
4, 1980 by the University of Minnesota Division of Epidemiology.  A copy
is included in Appendix VI.
III.   Analysis 3.
      A.   Introduction
          As the first analysis did not address the possible confounding
influence of urban pollutants in the examination of mean fetal, neonatal,
infant, and all sites cancer death rates for "lake bordering" versus "non-
lake bordering" counties, an examination of the same data sets was initiated
omitting all counties within the Great Lakes states having population cen-
ters greater than 100,000 inhabitants.

                                     56

-------
     B.  Methods




          A straightforward approach to utilizing the prior results was  to




identify the percent of rural "lake bordering" counties versus rural "non-




lake bordering" counties, for the Great Lakes Basin, which demonstrated




either one standard deviation excess or one standard deviation- deficit




from respective state means for 1977 white male esophageal, stomach, lung,




and all cancer mortality rates.  An identical evaluation was performed




for the percent live births with congenital anomalies, fertility rates,




neonatal death rates, and fetal death rates when  data were available.




     A total of 37 counties having population centers of greater than




100,000 inhabitants were omitted from the analysis leaving 72 "rural" lake




bordering and 538 "rural" non-lake bordering counties in the states of




Illinois, Indiana, Michigan, Minnesota, New York, Ohio, Pennsylvania, and




Wisconsin.




     C.   Results




          Table 13shows the percentages of rural "lake bordering" counties




having at least one standard deviation higher mortality/morbidity and those




with at least one standard deviation lower mortality/morbidity than their




respective state mean rates.  A larger percent of "lake bordering" counties




had rates in excess of one or more standard deviations (as compared to the




respective state means) than did "non-lake bordering" counties for the 1977




white male cancer sites of esophagus^ stomach,  bronchus,  trachea and  lung,  and




all-sites combined. The corresponding values are 16.67%, 13.9%,  8.3%,  and 9.72%




versus3.s%,  3.9%,.3.53%,  and 3.16%  respectively.  The percent of counties




with rates for "Live Births With Congenital Anomalies", demonstrated an




opposite trend with a greater percentage of "non-lake bordering" counties




having rates equal to or greater than one standard deviation than "lake





                                     57

-------
                                      TABLE 13

Percent of Lake Bordering and  Non-Lake Bordering  Counties Having Morbidity/Mortality
             Rates Discrepant  by £l Standard Deviation From State Means




Percent of 72 "lake
bordering" -counties
having rates 2 1
standard deviation
higher than their
respective state
mean rates
Percent of 72 "lake
bordering counties
having races a 1
standard deviation
Lower than their
respective state
mean rates
Percent of 538 "non-
lake bordering"
counties having rates
^ 1 standard devia-
tion higher than
their respective
state mean rates
Percent of 538 "non-
lake bordering".
counties having rates
> 1 standard devia-
tion lower than their
respective state
munii mcua 	
Cancer Sites
Bronchus,
Trachea, All
Esophagus Stomach & Lung Cancers



16.67






4.17






3.5






7.8






13.9






4.17






3.9






6.7






8.3






6:9






3.53






6.51






9.72






4.17






3.16






7.06



Reproductive Parameters
Z of Live Births Fetal
Fertility with Congenital Neonatal Daath
Rate Anomalies Daarh Rate Rate


%







*






*






*






3.64






7.27






7.43






6.86






4.69






9.38






6.25






8.56






7.81






7.81






6.0






10.4



                                                          *  rtata unavailable

-------
bordering" counties.  The percent of counties with rates above  one standard




deviation and below one standard deviation for "lake bordering" as compared




with "non-lake bordering" are:   3.64% above and 7.27% below, and 7.43% above




and 6.86% below one standard deviation, respectively.  The percentage values




for the "Neonatal Death Rate" are 4.69 and 9.38, and 6.25 and 8.56, respec-




tively.  The pattern of percentages for the "Fetal Death Rate" is the same




as the pattern for  the cancer sites.  The corresponding values are:   7.81




and 7.81, and 6.0 and 10.4.  The fertility rates for the eight states




were not analyzed due to inconsistent reporting between states.




     D.  Discussion




          The results of this analysis indicate that when comparing




counties without population centers of 100,000 inhabitants to the state




mortality/morbidity rates, the "lake bordering" counties have a greater




probability of experiencing extreme rates in those outcomes of morbidity/




mortality reviewed than do "non-lake bordering" counties.  For example, a




rural "lake bordering" county is 5.46 times as likely as a rural "non-




lake bordering" county to have a 1977 white male esophageal cancer rate one




standard deviation above its respective state mean.  Hypotheses drawn from




this third anlaysis should be guarded.  The cancer rates are for white




males in 1977 and may not be indicative of cancer mortality patterns of




other years, races, or women.  The available state vital statistics data re-




garding reproductive parameters were limited to a few states, therefore, results



are based on a small number of reporting counties.




IV.  Analysis 4.




     A.  Introduction




          Since the results cited above warranted further investigation,




the analytical strategies were refined so that comparisons could be made





                                    59

-------
between the rural "lake bordering" versus the rural "non-lake bordering"




county age-adjusted cancer mortality rates for specific anatomical sites.




     B.  Methods



          For each of the eight states in the Great Lakes Basin an average




age-adjusted site-sex-race specific cancer mortality rate per 100,000 was




computed for the rural "lake bordering" counties and the rural "non-lake




bordering" counties.  The mean of the eight averaged age-adjusted site




specific cancer mortality rates per 100,000 people was calculated to pro-




duce a summary rate for "lake bordering" counties of the Great Lakes Basin.




This procedure was followed to provide similar summary values for "non-




lake bordering" counties.




     C.  Results



        Table 14 presents the summary mean rates, their standard deviations,




and the differences and ratios between means for the rural "lake bordering"




and the rural "non-lake bordering" counties.




     The ratio of "lake bordering" to "non-lake bordering" mean age-adjusted




cancer rates  (hereafter termed ratio) of the esophagus for white males is




1.468.  The corresponding ratio for white females is 1.0374 and .5134 for




both non-white sexes.  The ratios for stomach cancer rates are 1.2513 for




white males,  1.1116 for white females, and 1.0759 for non-whites of both




sexes.  The corresponding ratios for bronchus, trachea, and lung cancers




are 1.1521 for white males, .9979 for white females, and 1.0691 for non-




whites of both sexes.  The corresponding ratios for the category of all




malignant neoplasms are 1.09777, 1.0339, and 1.0734 respectively.




     The ratio for whites demonstrating the largest relative disparity




(47%) between "lake bordering" and "non-lake bordering" counties is for




esophageal cancer in white males (1.468).  The second largest relative





                                     60

-------
                                       TABLE  M

Means, Standard DoviationB,  and Comparisons, of Mean Age-Adjusted  Causo-Sox-Raca-Speciftc
          Mortality Ratos Per 100,000 for Rural "Lake Bordering"  and  Rural
              "Non-Lake Bordering" Counties of the Great Lakes  Basin.

^.in rural "L.ike Bordering" mortality
rate fnr alt 8 statfts. (fl)
SlJndarJ Deviation
Me .in rur.it ":lr-n take Bordering" Bortality
: r.itc (or all 8 stales. CO
II
•', StanjArd 'deviation of summary aeon
difference of: (I - N)
1 ,r
Ratio of: "/-j
Eaupliageol Cancer
Hliltc
H
4.44
.1161
2.99
.4649
1.6523
1.468
F
.796
.3003
.768
.1424
.0287
Both
Sexes
J.24
.8336
3.756
.5652
1.481
1.0374 j 1.39«1
Don
White
Both
Sexes
6.08
3.8835
11.836
19.725
-5.759
.5134
Stoasch Cancer
White
M
18.55
3.2833
14.824
2.3426
3.725
1.2513
F
8.41
1.6585
7.561
1.3468
.8436
1.1116
Both
Sexes
26.97
4.548
22.385
3.6332
4.584
1.2048
Han
White
Both
SOXCB
2.4.29
12.958
22.579
9.8951
1.714
1.0759
Bronchus, Trachea t Lung Cancer
White
H
33.28
5.487
28.888
6.008
4.391
1.1521
T
5.08
.9448
5.094
.6288
-.0108
.9979
Roth
Sexes
38.36
6.2895
33.982
6.5547
Non
Ul.lt e
Bath
Sexes
12.59
25.8836
10.482
12.6605
4.383 1 2.1851
1.129 1.0691
All Neoplssas
White
H
170.15
7.8171
IJ5.184
9.0131
15.167
1.0977
; Both
' 1 S
-------
disparity (252)  is for stomach cancer in while males.   The  remaining




ratios have values approaching unity.  The cancer ratios for non-whites




should be viewed sceptically as they are based upon very small numbers of deaths.




     D.  Discussion




     These results indicate that white populations of  "lake bordering"




counties experience larger averaged cancer rates for every  category examined,




(except for bronchus', trachea and lung cancers in females)  than did white




populations of "non-lake bordering" counties.  The methodological approach




of comparing average cancer rates,  as opposed to the percentage of counties




with rates greater than one standard deviation above respective state means;




ia a more direct approach in examining the amount of difference in the




mortality experiences of the two geographical areas.




     The results of the third and fourth analyses suggest that when counties




with large urban centers are removed from consideration,specific sites show higher




cancer mortality in those counties which border the Great Lakes than in




those counties which do not border the Great Lakes.  The findings of the




four analyses were consistent in  revealing  elevated esophageal and stom-




ach cancer mortality rates in "lake bordering" counties.




V.  Analysis  5.




    A.   Introduction




          A fifth evaluation of the 1550-1969 county cancer mortality rates




was undertaken to determine if the above disparities would  be demonstrated




under a decidedly more complex and costly analytical method.  Animal




toxicity studies have demonstrated that many types of cancers may result




from exposure to organic chemicals.  Therefore, it was decided to examine




rigorously all thirty-five cancer sites reported in the NIH county 1950-




1969 cancer mortality data (.80) .  The analytical procedure encompassed the





                                     62

-------
following methods:




     B.   Methods




     1.  All age-adjusted site-race-sex-specific cancer rates for each




         county in the eight Great Lakes States were extracted from the




         NIH 1950-1969 county cancer mortality data set ©0).




     2.  The counties of the eight Great Lakes states were divided into




         three "Orders."  First Order counties are those counties which




         are adjacent to the Great Lakes ("lake bordering counties").




         Second Order counties are those counties adjacent to these "lake




         bordering"counties.  Third Order counties are considered to be




         all remaining counties of the eight Great Lakes states.  The




         listing of state counties by Order is presented in Table 15.




     3.  The 1960 population census figures were obtained for each of the




         three Orders.  Population counts within each Order were totaled




         for white males, white females, non-white males, and non-white




         females.  (See Tables 16and 17).  The population counts for 1960




         were used in a later step as weighting factors for the refinement




         of summary cancer rates.  The 1960  census figures were utilized




         as this year represents the midpoint of the twenty year data




         collection period for the NIH 1950-1969 county cancer mortality




         data set ( 80 ).




     4.  The age-adjusted site-race-sex specific county cancer mortality




         rates were summed within each Order and then divided by the total




         number of counties within the respective Order to yield average




         age-adjusted site-race-sex specific mortality, rates for each of




         the three Orders.




     5.  "Risk" ratios were then calculated comparing Orders one to two,





                                     63

-------
                                 TABLE  15

       Counties Designated as the 1st, 2nd, and 3rd Orders by State

                1st Order = Lake Bordering Counties
                2nd Order * Adjacent to Lake Bordering Counties
                3rd Order = Inland Counties (remaining counties)
Pennsylvania

1st

Erie
2nd
Crawford
Warren
                1st Order =• 1
                2nd Order » 2
                3rd Order = 64
                Total number of counties »  67
Counties omitted for "rural" analysis

1st                         2nd

•Erie                        —
                1st Order = 0
                2nd Order = 2
                3rd Order = 60
3rd

(All remaining)
 Illinois

 1st
 Lake
 Cook
                        3rd
                        Allegheny
                        Philadelphia
                        Lehigh
                        Lackawanna
                Total number of counties
                62
                 1st Order
                 2nd Order
                 3rd Order
2nd
Will
Dupage
Kane
McHenry

2
4
96
3rd
(All remaining)
                 Total number of counties  *  102
                                     64

-------
                           TABLE  15 (Continued)
Counties omitted for "rural" analysis

1st                         2nd                     3rd

Cook                        —                      Winnebago
                                                    Peoria

                1st Order » 1
                2nd Order « 4
                3rd Order - 94

                Total number of counties - 99

Indiana

1st                         2nd                     3rd

Lake                        St. Joseph              (All remaining)
Porter                      Starke
La Porte                    Jasper
                            Newton
                1st Order - 3
                2nd Order - 4
                3rd Order » 85

                Total number of counties « 92
Counties omitted for "rural" analysis

1st                         2nd                     3rd

Lake                        St. Joseph              Allen
                                                    Marion
                                                    Vanderburgh
                1st Order « 2
                2nd Order « 3
                3rd Order - 82
                Total number of counties = 87
                                     65

-------
                            TABLE 15 (Continued)
Michigan

1st                         2nd                     3rd

Berrien                     Cass                    (All remaining)
Van Buren                   Kalamazoo
Allegan                     Barry
Ottawa                      Kent
Muskegon                    Newaygo
Oceana                      .Lake
Mason                       Wexford
Manistee                    Kalkaska
Benzie                      Otsego
Leelanau                    Montmorency
Grand Traverse              Oscoda
Antrim                      Ogemaw
Charlevoix                  Gladwin
Emmet                       Midland
Cheboygan                   Saginaw
Presque Isle                Genesee
Alpena                      Lapeer
Alcoma                      Oakland
IOSCQ                       Washtenaw
Arenac                      Lenawee
Tuacola                     Iron
Huron                       Dickinson
Bay
Sanilac
St. Glair
Macomb
Wayne
Monroe
Gogebic
Ontonagon
Hbughton
Keweenaw
Baraga
Marquette
Alger
Luce
Chippewa
Mackinac
Schoolcraft
Delta
Menominee
                1st Order = 41
                2nd Order = 22
                3rd Order - 20

                Total number of counties - 83
                                     66

-------
                            TABLE 15 (Continued)
Counties omitted for "rural" analysis

1st                         2nd                     3rd

Macomb                      Genesee                 Ingham
Wayne                       Kent

                1st Order • 39
                2nd Order » 20
                3rd Order - 19

                Total number of counties « 78


Minnesota

1st                         2nd                     3rd

Cook                        Koochiching             (All remaining)
Lake                        Itasca
St. Louis                   Aitkin
                            Carlton

                1st Order - 3
                2nd. Order - 4
                3rd Order - 80

                Total number of counties « 87
Counties omitted for "rural" analysis

1st                         2nd                     3rd

St. Louis                   —                      Hennepin
                                                    Ramsey
                1st Order - 2
                2nd Order « 4
                3rd Order •* 78

                Total number of counties » 84
                                     67

-------
                            TABLE 15 (Continued)
New York

13C                         2nd                     3rd
St. Laurence                Franklin                (All remaining)
Jefferson                   Herkimer
Oswego                      Lewis
Cayuga                      Oneida
Wayne                       Madison
Monroe                      Onondaga
Niagara                     Cortland
Erie                        Seneca
Chautaugua                  Ontario
Orleans                     Livingston
                            Genesee
                            Wyoming
                            Cattaraugus
                            Tompkins
                            Hamilton

                1st Order » 10
                2nd Order - 15
                3rd Order - 33
                *Total number of counties =• 58
Counties omitted for "rural" analysis

1st                         2nd                     3rd
Erie                        Onandaga                Albany
Monroe                                              New York
                                                    Westchester

                1st Order - 8
                2nd Order » 14
                3rd Order - 30

                Total number of counties » 52
 *  The  "U.S.  Cancer Mortality by County  1950-1969"  includes Bronx,  Kings,
 Queens,  and Richmond  counties as New York.  All these counties will  be
 omitted  in  the  "rural" analysis.
                                     68

-------
                           TABLE  15 (Continued)
Ohio

1st                         2nd                     3rd
Lucas                       Fulton                  (All remaining)
Ottawa                      Henry
Erie                        Wood
Lorain                      Seneca
Cuyahoga                    Huron
Lake                        Ashland
Ashtabula                   Medina
Sandusky                    Summit
                            Trumbull
                            Geauga

                1st Order » 8
                2nd Order - 10
                3rd Order - 70

                Total number of counties » 88
Counties omitted for "rural" analysis

1st                         2nd                     3rd

Lucas                       Summit                  Hamilton
Cuyahoga                                            Mahoning
                                                    Montgomery
                                                    Stark
                                                    Franklin

                1st Order » 6
                2nd Order « 9
                3rd Order - 65

                Total number of counties * 80
                                     69

-------
                            TABLE  15  (Continued)
 Wisconsin

 1st                         2nd                     3rd
 Douglas                     Burnett                 (All remaining)
 Bayfield                    Washburn
 Ashland                     Sawyer
 Iron                        Price
 Marinette                   Vilas
*Menominee                   Florence
 Door                        Forest
 Brown                       Langlade
 Rewaunee                    Calumet
 Manitowoc                   Fond du Lac
 Sheboygan                   Outagamie
 Ozaukee                     Washington
 Milwaukee                   Wau kes ha
 Racine                      Walworth
 Renosha

                 1st Order =• 15
                 2nd Order = 14
                 3rd Order =• 41

                 Total number of counties = 70
 Counties omitted for "rural" analysis

 1st                         2nd                     3rd
 Milwaukee                   —                      Dane

                 1st Order = 14
                 2nd Order - 14
                 3rd Order = 40

                 Total number of counties - 68
 *Note:  Menominee County did not exist in 1960.  In:  "U.S. Cancer Mor-
         tality by County 1950-1969," Menorainee includes Oconto and
         Shawano counties.  For purposes of this analysis Menominee
         will be defined as lake Bordering with a population comprised
         of Oconto and Shawano counties.
                                      70

-------
                                         TABLE 16
    SUMMARY POPULATION FIGURES FOR THE- GREAT LAKES BASIN BY ORDER, SEX, AND RACE (1960)
State
   1st Order
     White
Male       Female
  2nd Order
    White
Male     Female
   3rd Order
     White
Male       Female
PA
MN
WI
MI
IL
IN
NY
OH
119,286
123,469
858,142
1,947,589
2,224,186
291,740
1,161,664
1,174,091
Total:
Male + Female -
PA
MN
WI
MI
IL
IN
NY
OH
Total








:








1
124,271
122,868
880,945
1,970,115
2,297,648
284,230
1,217,220
1,225,393
7,900,167 8,122,690
16,022,857
Nonwhite
Male Female
3
1
39
285
432
45
59
156
,024
'Male -t- Female «
Totals:


PA
MN
WI
MI 2,
IL 2,
IN
NY 1,
OH JLj.
Total: 8,
Male +
Female **
Male and
1st
Male
122,751
124,653
897,671
232,741
656,995
337,117
221,628
330,983
924,539

,465
,184
,529
,152
,809
,377
,964
,892
,372 1
3,660
1,146
40,254
300,536
468,738
47,312
61,155
167,526
,090,327
59,727
48,766
267,546
1,092,906
386,717
136,211
628,895
518,444
62,639
45,797
267,636
1,115,276
392,361
136,232
642,894
532,039
3,139,212 3,194,874
6,334,086
Nonwhite
Male Female


2,
60,
10,
7,
15,
28,
124,
2,114,699
Female by
Order
Female



2,
2,

1,
1',
9,

18,137,
127,
124,
921,
270,
766,
331,
278,
392,
213,

556
931
014
199
651
386
542
375
919
017

614
912
122
232
768
007
239
092
986
248.,
558
815
2,166
61,396
7,686
7,419
14,022
29,120
123,182
168
4,914,866
1,499,258
792,511
479,927
1,824,784
1,737,558
5,630,805
2,683,591
5,173,215
1,531,445
792,123
480,052
1,884,556
1,802,583
6,005,593
2,776,140
19,563,300 20,445,707
40,009,007
Nonwhite
Male Female
411,893
19,373
4,662
17,062
73,602
80,405
626,672
203,118
1,437,227
3,011
Order and State
2nd
Male
60,341
49,678
269,668
1,153,138
397,485
143,218
644,134
564,536
3,264,198

Order

Female
63,
46,
269,
1,176,
400,
143,
656,
561,
3,318,

197
631
802
672
047
651
916
159
056

6,582,254
3rd
Male
5,326,759
1,518,631
797,173
496,989
1,898,386
1,818,403
6,257,477
2,886,709
21,000,527

Order
Female
5,618,387
1,550,276
796,264
- 493,003
1,961,859
1,888,567
6,723,774
2,988,091
22,020,221

445,172
18,831
4,141
12,951
77,303
8 5, '984
718,181
211,951
1,574,514
,741
State











Population
Totals
11,319,
3,413,
3,951,
7,823,
10,081,
4,662,
16,782,
9,706,
67,740,

366
864
777
194
158
498
304
397
558

43,020,748
                                            71

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                                         TABLE  17
     SUMMARY POPULATION  FIGURES FOR THE GREAT LAKES BASIN BY ORDER, SEX, AND RACE  (1960)
         Counties with urban centers containing populations >100,000 are omitted
 State
            1st Order
              White
         Male       Female
             2nd Order
              White
          Male       Female
            3rd Order
              White
          Male       Female
PA
MN
•wi
MI
IL
IN
NY
OH
Total:
Male +
PA
MN
WI
MI
IL
IN
NY
OH
Total:
Male +
Totals:



PA
MN
WI
MI
IL
IN
NY
OH
otal: 2,









Female *









Female »
0
8,799
384,322
695,625
147,938
75,954
410,795
297,147
2,020,580
4,035
0
110
6,608
21,737
6,401
2,861
8,830
11,191
57,738
112
0
8,078
385,501
692,888
133,023
74,375
421,326
299,504
2,014,695
,275
0
92
6,398
21,041
6,294
2,200
7,977
11,234
55,236
,974
59,727
48,766
267,546
755,820
386,717
24,526
428,824
286,631
2,258,557 2
4,543,
614
912
2,122
34,300
10,768
40
8,183
7,734
64,673
125,
62,639
45,797
267,636
767,188
392,361
23,654
434,031
291,950
,285,256
813
558
815
2,166
35,002
7,686
35
6,984
7,811
61,057
730
3,264,038
905,024
683,775
379,688
1,639,041
1,267,409
1,958,806
1,519,389
11,617,170
23,533
91,388
6,004
3,114
12,849
64,395
22,522
63,116
47,247
310,635
621
Male and Female by Order and State

1st
Male
0
8,909
390,930
717,362
154,339
78,815
419,625
308,338
078,318

Order
Female
0
8,170
391,899
713,929
139,317
76,575
429,303
310,738
2,069,931

2nd
Male
60,341
49,678
269,668
790,120
397,485
24,566
'437,007
294,365
2,323,230

Order
Female
63,197
46,612
269,802
802,190
400,047
23,689
441,015
299,761

3rd
Male
3,355,426
911,028
686,889
392,537
1,703,436
1,289,931
2,021,922
1,566,636
2,346,313 11,927,805

Order
Female
3,496,554
892,500
684,453
386,159
1,758,000
1,321,482
2,095,528
1,593,176
12,227,852
3,403,683
886,921
681,517
377,182
1,690,666
1,299,659
2,029,110
1,547,862
11,916,600
,770
92,871
5,579
2,936
8,977
67,334
21,823
66,418
45,314
311,252
,887

State
Population
Totals
6,975,518
1,916,897
2,693,641
3,802,297
4,552,624
2,815,058
5,844,400
4^73,014
32,973,449
Male +
Female
4,148,249
4,669,543
24,155,657
                                             72

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         one to three,  and  two  to  three.   A "risk"  ratio was defined as  the




         division of an averaged age-adjusted  site-race-sex specific cancer




         mortality rate for a given Order  divided by  the corresponding




         average rate "of a  different Order.




     6.   Each average age-adjusted site-race-sex specific  cancer mortality




         rate was then  multiplied  by the census figure £o*-the respective




         race, sex, and Order.  This product was then divided  by the total




         population figure  for  the respective  sex and race of  the  eight




         Great Lakes states. This quotient represents a population weight-




         ed average age-adjusted  site-race-sex specific cancer mortality




         rate by Order.




     7.   "Weighted'risk" ratios were calculated comparing  the  average rate




         for each of the Orders to the overall rate for the eight  Great




         Lakes .states.   A "weighted risk"  ratio was defined as the division




         of the average age-adjusted site-race-sex  specific cancer mortality




         rate for a given Order by the sum of  the  three weighted average




         age-adjusted site-race-sex specific cancer mortality  rates  for  a




         given Order.








(The calculations and data  derived from steps  4 through 7  will hereafter




be termed the "urban and rural" analysis.)




     8.   Steps 4 through 7  were repeated omitting  all counties within




         the eight Great Lakes  states having population centers of 100,000




         or more inhabitants.




(The calculations and data  derived from step 8 will hereafter  be termed  the




"rural" analysis.)




     9.   The average age-adjusted  site-race-sex specific  cancer mortality





                                     73

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         rates for the "rural" and "urban and rural" analyses were examined

         for disparities of increasing or decreasing mortality rates by

         degree of geographical contiguity to the Great Lakes,  (e.g.,

         Which cancer sites have higher mortality rates in the first Order

         counties than second Order counties, which in turn have higher

         rates than the third Order counties?)

     C.   Results

          The rates  computed for each of the thirty-five sites for both

the "urban and rural" and "rural" analyses are presented in Appendix I,

A summary of Appendix I is given below.  Only the most extreme ratio

values for non-white populations will be presented as most non-white rates

were based on relatively small numbers of deaths.


         1.   "Urban and Rural" Analysis

     "Risk" Ratios:  Order 1 cour.ties had a 31% excess of esophageal cancer

mortality in white males and a 2(>/i excess in white females over Order 2

counties.  A 68% excess in mortality due to cancer of other endocrine

organs for white females and a 46% excess for breast cancer mortality in

white males was noted in Order 1 over Order 2.  The "risk" ratio of Order

1 to Order 2 county nasopharyngeal cancer mortality rates was 3.66 for

non-white males and 7.74 for non-white females.  The corresponding ratios

for esophageal cancer in non-whites was 1.64 and 0.04 respectively.  Non-

white rate ratios for Order 1 to Order 2 counties were 5.36 for thyroid

cancer in males and 8.59 for brain and other nervous system cancers in

females.

     A "risk" ratio of 40.14 in non-white females for esophageal cancer
 3)  All rates, ratios, and percentages quoted in this analysis are in
    respect to mortality and not incidence.


                                     74

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mortality was noted when comparing Order 2 to Order 3 counties.  White males

experienced 42% higher recorded thyroid gland cancer mortality in Order 1

than Order 2.

     The "risk" ratios comparing rates in Order 1 to Order 3 counties indi-

cated an excess of esophageal cancer mortality for white males of 44%; for

white females, 32%; non-white males, 41%; and 58% for non-white females.

The corresponding values for stomach cancer were 35%, 24%, 79%, and 33%

respectively.  The ratio of rates for Order 1 to Order 3 counties for lip

cancer mortality in non-white females was 5.33, 5.73 for "other" skin can-

cers in non-white males, and 8.05 for lymphosarcoma* in non-white males.

     "Weighted Risk" Ratios:  The ratio of the rates in Order 1 counties

to the sum of all the weighted rates for esophageal cancer mortality were

1.28 for white males, 1.21 for white females, and 1.22 for non-white males.

The corresponding ratios for stomach cancer were 1.22, 1.16, and 1.34

respectively.  White females demonstrated a risk of 0.1116 for mortality

due to cancer of the nose, nasal cavities, middle ear, and accessory

sinuses.  The "weighted risk" ratio for lymphosarcoma* in non-white males

was 2.08.  Non-white females had a ratio of 2.56 for cancers of the con-

nective tissues.

     The mortality ratio of rates in Order 2 counties to the sum of all

the weighted rates for non-white males  was 2.21 for rectal cancer, 5.64

for breast cancer, 3.22 for cancers of the eye, 5,06 for bone* cancers, and

3.88 for Hodgkin'^s disease.  The corresponding ratios for non-white females-

were 2.62 for salivary gland cancers, 13.58 for esophageal cancer, and

2.07 for melanomas of the skin.


*  See the NIH publication  (80 ) for a complete listing of cancers included
   in this category.


                                     75

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     The ratio of rates ±a Order 3  counties to the sum of  all the weighted


rates for non-white male tongue and mouth cancers was 1.46.   Non-white


females demonstrated a ratio of 4.50 for cancers of the connective tissue.


         2.   "Rural" Analysis


     "Risk"Ratios:  White males experienced 27% more salivary gland cancer,


26% more esophageal cancer, and 48% more breast cancer mortality in Order


1 counties than in Order 2 counties.  Order 1 county white females experi-


enced proportionately greater mortality than Order 2 county white females


for salivary gland cancer (20%), esophageal cancer (27%),  nose and middle


ear* cancers (30%), and cancer of the endocrine organs (73%).  The esoph-


ageal "risk" ratios for non-whites was 1.78 for males and  0.03 for females.


The corresponding non-white ratios for cancer mortality due to brain and


other nervous system cancers were 2.02 and 10.52.  The percent of excess


mortality in Order 1 over Order 2 counties for non-white males was 393%


for nasopharyngeal cancer, 188% for other skin cancers* 102% for brain

                        *
and other nervous system cancers, 152% for thyroid cancers, 279% for lympho-


sarcoma and 122% for I.C.D. codes not listed.*  Non-white  females also


experienced proportionately greater mortality in Order 1 than Order 2


counties for the cancer sites of pancreas (332%), nose and middle ear*


(120%), brain and other nervous system* (951%), Hodgkin's  disease (114%),


and multiple myeloma  (222%).


     The "risk" ratios comparing rates in Order 2 to Order 3 counties have


the following values:  for white males the thyroid cancer  mortality ratio


was 1.44; non-white male mortality ratios were 1.91 for rectal cancer, 8.55


for breast cancer,  2.16 for bladder cancer, 2.27 for other skin cancers',
 *  See  the NIH publication ( 80) for a complete listing of cancers included
   in this category.


                                     76

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3.29 for cancer of the eye, 5.38 for bone cancer, and 5.59 for Hodgkin's

disease.  The corresponding ratios for non-white females included 2.13

for salivary gland cancers, 47.124 for esophageal cancer, 2.31 for stomach

cancer, and 2.58 for cancer of the cervix uteri.

     In the comparison of Order 1 to Order 3 counties, excess cancer mor-

tality was noted for the sites of esophagus (39%), stomach (36%), larynx

(31%), and thyroid gland (33%) for white males.  The corresponding percent-

ages for white females were esophagus (33%), stomach (24%), and endocrine

organs (42%).  Non-white males and females had respective values for excess

esophageal cancer mortality of 42% and 22%.  For stomach cancer mortality

for non-whites the excesses were 86% and 33% respectively.  The non-white

male additionally had excesses for the sites of nasopharynx (203%), other

skin (555%) and lymphosarcoma (906%).  Non-white females showed excess

cancer mortality for the sites of pancreas (201%), nose and middle ear*

(175%), and brain and other nervous system cancers (388%).

     "Weighted Risk Ratios":  The ratio of the rate in Order 1 counties, to

the sum of all the weighted rates for esophageal cancer mortality was 1.30

for white males and 1.27 for white females.  White males demonstrated

elevated risks in the first Order counties for the sites of stomach (1..27),

larynx (1.25), and thyroid (1.20).  A risk ratio of 1.38 was noted for

white female mortality from cancer of the endocrine organs.  Non-white

males had elevated risk ratios for the sites of nasopharynx (2.50), "other"

skin (3.39), and lymphosarcoma (4,10).  The sites of nose and middle ear*

and brain and other nervous system* had weighted risk ratios of 2.18 and

3.42, respectively, for non-white females.
*  See the NIH publication  (80) for a complete listing of cancers included
   in this category.

                                     77

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     The "weighted risk" ratios comparing site rates in Order 2 to the sum

of all the weighted rates showed minimal differences from "1.0" for white

males and females.  The largest departure from "1.0" was 1.30 for white

male thyroid cancer mortality.  Non-white males had risk ratios of 4.03

for breast cancer mortality, 2.7 for mortality due to eye cancer-, 3.4 for

bone cancer mortality and 3.24 for mortality due to Hodgkin's disease.  Non-

white females had elevated risk ratios for the sites of salivary glands

(2.00) and esophagus (6.19).

     The ratios of site rates in Order 3 counties to the sum of all the

weighted rates for white populations of the Great Lakes states did not

differ markedly from unity.  However, a 26% excess in non-white males and

a 25% excess in non-white females was noted for tongue and mouth* cancer

mortality.  A 36% excess was also shown for thyroid gland cancer mortality

for non-white males.

     The populations living in the Great Lakes basin would be expected to

experience varying degrees of exposure to lake contaminants.  Exposure

potentials are based upon many factors including occupational exposure,

drinking water sources, landfill contamination, contamination of ambient

air, and dietary habits.  Those individuals who may be exposed to contam-

 inants at levels which may produce disease  may comprise a small  subset of

 the entire population.  Therefore, the excess cancer mortality contributed

 by the "high risk" groups may not dramatically increase the mortality

rates  in one Order of  counties over  those of another Order.  All average

rates were examined by site for evidence of increasing or decreasing mor-

tality by relative geographic proximity to the Great Lakes.  Tables 18andl9
 *   See  the NIH publication  ( 80 ) for a complete listing of cancers included
    in this category.


                                     78

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demonstrate increasing average mortality rates with increased proximity

to the Great Lakes for the "urban and rural" and "rural" analysis respec-

tively.

     Tables 20 and 21 list by race and sex those cancer sites which have

average cancer mortality rates which decrease in magnitude as one approaches

the Great Lakes.  In summary, twenty-nine race and sex specific sites were

shown to have increased with proximity to the Great Lakes and twenty decreased

in the "urban and rural" analysis.  In the "rural" analysis, twenty-seven

sites increased and twenty-three decreased with proximity to the Great Lakes.

      D.   Discussion

          In the "urban and rural" analysis comparing Order 1 counties to

those of the other Orders, white males experienced the highest mortality

from esophageal, stomach, breast, and thyroid cancers when .examining the

weighted and unweighted risk ratios.  The demonstration of excess mortality

in these sites for first Order.counties waa consistent with the "rural"

analysis.  White females had the greatest excess mortality for cancers of

the esophagus, stomach, and endocrine organs* in the "urban and rural"

analysis in Order 1 counties.  This pattern was also found in the "rural"

analysis.  The excesses in rates noted for the first Order county white

population were typically between 25% and 50% over those of the other Order

counties.  A "risk" ratio of two or greater was not found in the thirty-

five-sites examined.

     The non-white populations of the Great Lakes basin demonstrated ele-

vated risks for many sites in Order 1 counties.  For example, non-white fe-

males had a risk ratio of 10.06 for lymphosarcoma when comparing Order 1
*  See the NIH publication (80) for a complete listing of cancers included
   in this category.


                                     79

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                                  TABLE 18

       Cancer Sites Demonstrating INCREASING Average Mortality Rates
                   With Proximity to  the Great Lakes Basin
Site                                Race                Sex

Lip                                 m                  F
Nasopharynx                         W                   F
Tongue, Mouth                       W                   M
                                    W                   F
Esophagus                           W                   M
                                    W                   F
Stomach                             W                   M
                                    W                   F
                                    NW                  M
Large intestine                     w                   M
Liver                               NW                  M
                                    NW                  F
Pancreas                            W                   M
                                    W                   F
Nose, Middle ear                    NW                  F
Larynx                              W                   M
Trachea, Bronchus, Lung             W                   M
Breast                              W                   F
Corpus uteri                        NW                  F
Bladder                             W                   F
Other skin                          NW                  M
Endocrine organs                    w                   M
Lympho sarcoma                       .W                   M
                                    W                   F
                                    NW                  M
Multiple myeloma                    w                   F
Malignant neoplasms                 W                   M
                                    W                   F
                                    NW                  M
                                    80

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                                   TABLE 19

   Rural Analysis - Omitting Counties With Population Centers  }100,000

       Cancer Sites Demonstrating INCREASING Average Mortality Rates
                  With Proximity to the Great Lakes Basin

Site                                Race                Sex

Nasopharynx                         W                   F
Tongue, Mouth                       W                   M
                                    W                   F
Esophagus                           W                   M
                                    W                   F
Stomach                             W                   M
                                    W                   F
                                    NW                  M
Large intestine                     W                   M
Pancreas                            W                   M
                                    W                   F
Larynx                              W                   M
Trachea, Bronchus, Lung             W                   M
Breast                              W                   F
Corpus uteri                        NW                  F
Bladder                             W                   F

Other skin                          NW                  M
Endocrine organs                    W                   M

Lymphosarcoma                       W                   M
                                    NW                  M

All neoplasms                       W                   M
                                    W                   F
                                    NW                  M
Liver                               NW                  M
                                    NW                  F
Nose, Middle ear                    NW                  M
                                    NW                  F
                                    81

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                                   TABLE 20

       Cancer Sites Demonstrating DECREASING Average Mortality Rates
                  With Proximity to the Great Lakes Basin
Site                                Race                Sex

Lip                                 W                   F
Tongue, Mouth                       W                  M
                                    m                  F
Pancreas                            NW                  M
Trachea, Bronchus, Lung             W                   F
Corpus uteri                        W                   F
Ovary, Fallopian tube               NW                  F
Kidney                              NW                  F
Bladder                             NW                  F
Skin melanoma                       W                   M
                                    W                   F
Other skin                          W                   M
                                    W                   F
                                    NW                  F
Eye                                 W                   M
Bone                                W                   F
                                    NW                  F
Hodgkin's Disease                   W                   F
Leukemia                            W                   M
                                    W                   F
                                    82

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                                  TABLE 21

   Rural Analysis - Omitting Counties With Population centers  100,000

       Cancer Sites Demonstrating DECREASING Average Mortality Rates
                  With Proximity to the Great Lakes Basin
Site                                Race                Sex

Lip                                 W                   F
Tongue, Mouth                       NW                  M
                                    NW                  F
Large intestine                     W                   F
Pancreas                            NW                  M
Larynx                              NW                  F
Trachea, Bronchus, Lung             W                   F
Corpus uteri                        W                   F
Bladder                             NW                  F
Skin melanoma                       NW                  M
                                    W                   M
                                    W                   F
Other skin                          W                   M
                                    W                   F
                                    NW                  F
Eye                                 W                   M
Bone                                W                   F
                                    NW                  F
Leukemia                            W                   M
                                    W                   F
Ovary, Fallopian tubes              NW                  F
Kidney                              NW                  F
Hodgkin's disease                   W                   F
                                    83

-------
counties with the Order 3 counties in the rural analysis.   The Order 1

county average rate was based on twenty-five reported deaths over the twen-

ty year period.  The point of the example is that many of  the extreme rates

reported here for non-whites are based on very small numbers of reported

deaths.  Each ratio reported in Appendix  I  should be evaluated indepen-

dently by a critical review of both the numerator and denominator data.

The accuracy and consistency of the reporting of cancer deaths in the non-

white populations between 1950 and 1969 (93) are subject  to question.

Conclusions drawn from the non-white ratios would be highly speculative

at best.  The reader is cautioned since incidence, as opposed to mortality,

data are typically evaluated in studies of disease etiology.

     In conclusion, the fifth analysis supports prior analyses which

indicate that white populations in first Order counties experienced a

higher rate of mortality due to esophageal and stomach cancers than in

second and/or third Order counties.  These excesses remained when large

urban centers with, their possible confounding factors were removed from

the analysis.  Furthermore, these disparities persisted when a second

analysis was performed which weighted each rate by the respective popula-

tion count.  Excess mortality due to cancers of the breast in white males

and endocrine organs* in white females in first Order counties were also

noted in this analysis.
 *   See  the NIH data set (80) for a complete listing of cancers included
    in this category.

                                    84

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      A Pilot Study to Determine the Feasibility "of an Epidemiologic
         Investigation Among Commercial Fishermen of the Effects
                 of Polychlorinated Biphenyls on Health
I.  Background and Rationale

    A critical concern with respect to the presence of polychlorinated

biphenyls (PCBs) in the environment is the introduction of these

compounds into the human food chain.  However, there is limited

information regarding PCB exposure to the general population via food

consumption.  Jelink and Corneliussen report that for the period

between 1969 and 1975, there have been significant decreases in the

PCB levels in all food commodities with the exception of fish  (60).

Data from the U.S. Fish and Wildlife Service. (U.S.F.W.S.) regarding

PCB concentrations in fish species sampled from the Great Lakes indicate

that the average PCB concentrations for several fish species exceed

recommended F.D.A. levels* for human consumption.  Average PCB con-

centrations were noticeably higher in fish species taken from Lake

Michigan than those taken from other lakes.  It must be noted however,

that these averages are based upon small sample sizes and no trends

could be established from year to year.

     Graham reported on the levels of PCB in the edible portion of

commercial fish species from the Canadian waters of the Great Lakes.

His results indicate that several commercial species have mean PCB

concentrations greater than 1 ppm.  Table 22 summarizes his findings.




*  Current F.D.A. tolerance levels for PCBs in fish  =   5 ppm

   Recommended F.D.A. tolerance levels for PCBs in fish = 2 ppm

                                    85

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                            TABLE 22

            Levels of PCBs in Canadian Fish Species
                                                           Mean PCB
                           Fish Species               Concentration (ppm)

                           lake herring                      1.17
                           lake trout                        2.02

                           chub                              2.09
                           carp                              1.55
                           sucker                            1.33
                           coho salmon                       5.11

Erie                       alewife                           1.22
                           carp                              1.27
                           yellow pickerel                   1.16
                           white bass                        1.26
                           catfish                           2.04
                           coho salmon                       3.14

Ontario                    yellow perch                      1.23
                           smelt                             4.16
                           white perch                       1.84
                           carp                              1.69
                           rock bass                         1.76
                           eel                              17.14
                           coho salmon                       4.97
Graham, J.M.  "Levels of PCBs in Canadian Fish Species."  in: National
Conference on Polychlorinated Biphenyls, November 17-21, Chicago,
Illinois.  E.P.A. 560/6-75-004, March 1976.
                                 86

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     The State of Michigan has collected data regarding PCS concentrations




in fish taken from Lake Superior.  Results indicate that lake trout have the




greatest PCS body burdens and exceed recommended F.D.A. levels in many in-




stances.  It is interesting to note that the sampling stations used to collect




Lake Superior fish species are adjacent to counties in the upper peninsula of




Michigan which are relatively free of industries normally associated with PCS




discharges.  This suggests several possible explanations including:




     1)  Fish may spend part of their life cycle in highly polluted waters




         and then migrate to other portions of the lake.




     2)  Non-point sources of PCBs (e.g., atmosphere, water column, bottom




         sediments) have a much greater impact on fish body burdens than




         previously expected.




     3)  There are unidentified sources of PCBs resulting in increased ex-




         posure to fish in these areas.




     4)  Fish taken from waters adjacent to heavily industrial areas may




         have much greater PCS body burdens.




     The Region V office of the U.S.E.P.A. is currently evaluating data re-




garding PCS levels in fish taken from Lake Michigan. Preliminary results in-




dicate the fish from southern Lake Michigan have greater PCB concentrations




than those from northern Lake Michigan.  This is not surprising due .to the




magnitude of industrialization around southern Lake Michigan waters.




     Humphrey and his associates measured the PCB levels in persons consuming




sport fish caught from Lake Michigan.  These authors conclude that there could




be long-term accumulation of PCBs in individuals consuming large amounts of




fish (52).  However, at the time of their studies, no differences in health




status could be observed between participants (i.e., fish consumers) and




controls.  This does not negate the possibility of latent effects resulting




                                   87

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from chronic exposure which might be demonstrated at a later date.





     In summary,  it appears that the scope of PCB contamination of  the




food supply has narrowed to the point where fresh water fish are the primary




source of PCB exposure in the diet of fish-consuming individuals.  According.




to Jelink and Corneliussen the average daily dietary intake of fresh water




fish of the U.S.  citizen is low (60 ).   However, their reports do  not consider




population sub-groups which may consume significantly larger quantities of fish




than the general population and, therefore, may have a potentially  greater ex-




posure to PCBs.
                                     88

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 II.  Alms and Objectives

      'A.  General

          The intent of this pilot study was to evaluate three research proto-

      cols to determine the effectiveness and feasibility of an epidemiologic

      investigation of commercial fishermen.  Several issues were addressed,

      including:

          1.  Could the requisite study participants be found?

          2.  Would the participants respond to the study instruments (i.e.,

              questionnaires?)

          3.  Would one test protocol produce better responses than another?

          4.  Is the .information obtained from the study instruments accurate?

          5.  Could verification of the participant responses be obtained?

          6.  What is the distribution of fish consumption (both amount and

              type) in this cohort?

III.  Methods

      A.  Study Protocols

          Three detailed protocols were developed for the collection of infor-

      mation regarding the fishing practices and health status of commercial

      fishermen.  These included a telephone-survey protocol (Protocol I),

      a protocol involving a pre-mailing of a set of questions with a sub-

      sequent telephone-survey (Protocol II), and a mailing of a questionnaire

      protocol  (Protocol III).

          These protocols were tested using questionnaires and study corre-

      spondence developed by  the Great Lakes project staff.  Protocol effective-

      ness was determined by evaluating which method:

          1.  Produced the greatest proportion of questions answered in the

              correct format.

          2.  Produced the greatest participation rate.

          3.  Produced the most reliable responses as determined through validation.
                                       89

-------
B,   Identification and Selection of a Study Population




         Since the purpose of this study was to identify a population in the




     Great Lakes basin with potentially high PCS exposure and determine the




     feasibility of an epidemiologic investigation of that "high risk" popu-




     lation, several possible study populations were identified including




     sport fishermen, commercial fishermen, and sub-samples of the general




     population from areas contaminated with PCBs or other organics.  Based on




     the availability of licensing records and the recognition of the problems




     associated with determining PCB exposure in the general population,




     commercial fishermen were selected as the initial study population.




         Available commercial fishing licenses were requested fron the states




     of Minnesota, Wisconsin, Michigan, Illinois, Indiana, Ohio, Pennsylvania,




     and New York.  In addition to.the state listings other sources included




     commercial fishing organizations and publication membership lists which




     were especially useful in identifying retired commercial fishermen. In




     general, the most frequently contacted sources of information were the




     respective state licensing departments.  Table 23 illustrates the kinds of




     information obtained from each state regarding licensed commercial fisher-




     men.




         A potential problem with the use of current lists of commercial fisher-




     men is the omission of individuals who fish intermittently and purchase licenses




     on a less than yearly basis (as compared with a full-time fisherman who




     purchases a license every year) and recent retirees.  State departments were




     willing to send current lists of commercial fishermen but expressed con-




     cern over the feasibility of abstracting past records.  Great Lakes study




     members visited the Departments of Natural Resources in the states of




     Indiana and Wisconsin to evaluate the content of commercial fishing records.



                                      90

-------
                                        TABLE 23

          Sources  of  Information  Regarding  Commercial  Fishermen
State

New York



Illinois


Indiana



Pennsylvania


Minnesota




Ohio


Michigan
Wisconsin
                                                          Number of
Sources of information regarding                          individuals
commercial fishermen	                          with licenses

1. New York State Eepartment of Environmental  Conservation
 a) 1980 list of commercial fishermen—Lake  Erie                  25
                                     —Lake  Ontario               25

2. State tepartaent of Natural Resources
 a) 1980 list of commercial fishermen—inland waters             382

1. State Department of Natural Resources
 a) 1975-1981 list of commercial fishermen—Lake Michigan         66
 b) 1980 list of commercial fishermen—inland waters             239.

1. Pennsylvania Fish Commission Division  of  Fisheries
 a) 1979 list of commercial fishermen—Lake  Erie                  42

1. lepartment of Natural Resources
 a) 1979 list of commercial fishermen—Lake  Superior              89
2. Claude Ver Euin, Editor of The F. isherman
 a) Publication list of former commercial fishermen  (retired?)    25

1. Department of Natural Resources
 a) 1980 list of commercial fishermen—Lake  Erie                 134

1. Department of Natural Resources
 a) 1980 list of commercial fishermen—Lakes Michigan,
    Huron, Superior
2. Claude Ver Bain, Editor of The Fisherman
 a) Publication listing of commercial fishermen                   170

1. Department of Natural Resources
 a) 1974, 1976-1981 list of commercial fishermen in
    Lakes Superior, Michigan                                    449

                                              total            1,646

                                       less -  Inland Waters      621

                                              Great Lakes      1,025
Compiled by the Great Lakes Project Staff
                                           91

-------
     An additional 58 individuals or 90% of the state cohort were abstracted




from Indiana files and 202 individuals or 45% of the state cohort were ab-




stracted from Wisconsin files.  These results suggest that current license




holders do not accurately reflect the number of individuals who have commer-




cially fished in the recent past and that all state licensing departments should




be contacted in person to abstract all available records. In general, license




records are maintained from 3 to 5 years. However,  some states (e.g., Wisconsin)




maintain records for up to twenty years.




     For the purposes of the pilot study it was decided that the individuals ob-




tained from the various Information sources mentioned in Table 23 were suffi-




cient to test study protocols and instruments.




     A. total of 1,025 individuals were identified as licensed Great Lakes




commercial fishermen between the years 1974 to 1981;  The majority of commercial




fishermen are found in the state of Wisconsin and fish Lake Michigan.  In




addition to the Great Lakes commercial fishermen, there were large contingents of




inland commercial fishermen (i.e., individuals who  fish non-Great Lake associated




rivers and lakes in the states of Illinois and Indiana). These individuals totaled




621 but were not included in the pilot study.  The reasoning behind this omission




stemmed from the fact that their socio-economic status and racial composition




may be significantly different from that of Great Lakes commercial fishermen. It




was reported that a significant number of these individuals are black and the




majority come from a low socio-economic background. However, their incorporation




into a large-scale study should be considered, possibly with separate analytical




procedures.




     The study protocols and instruments were pilot tested using a randomly




selected sub-sample (n * 75) of Great Lakes commercial fishermen. Individuals were




stratified according to lake and state.  The number of individuals to be used in




the pilot study from each lake/state stratum was arbitrarily selected to insure




                                     92

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                                                 TABLE 24

                                Number of Commercial Fishermen  in  the
                                 Great Lakes Basin  by  Lake  and  State
     Lake
  States
 adjacent
to the lake
Number of fishermen
from each state who
   fish the lake
     Number
  of fishermen
randomly selected
	by state	
    Total
  number of
fishermen to
 be studied
  by lake
vo
CO
     Superior
     Michigan
     Huron

     Erie




     Ontario

     ^otals
Minnesota
Wisconsin
Michigan

Wisconsin
Michigan
Indiana
Illinois

Michigan

Ohio
Pennsylvania
New York
Michigan

New York
       114
        44
        25

       424
        76
        66
         0

        47

       134
        42
        25
         3

        25
      12
       6
       6

      12
       6
       6
       0
       6
       3
       3
       0
     24



     24




      9

     12
                          1,025
                                                                            75
                                                75

-------
          a representative  sample of commercial fishermen from the entire Great Lakes

          Basin region.  Table 24 describes the number of individuals available by lake

          and state.  Included in this table is an arbitrary number of randomly-selected

          individuals to be used in the pilot study.

               The sub-sample of Great Lakes commercial fishermen was divided and randomly

          assigned to each  of three study protocols with an equal distribution of individ-

          uals among each protocol.  The number of individuals assigned to each protocol was

          dependent on the  number of individuals arbitrarily selected from each state. The

          following diagram illustrates this procedure for Lake Superior as an example:
Protocol
                   Minnesota
                  12 individuals
                                         LAKE.SUPERIOR
 I   II    III
(4)    (4)   (4)
 I
(2)
      Wisconsin
     6  individuals
II   III
(2)  (2)
                          Michigan
                                                                  6  individuals
 I  II   III
(2)   (2)  (2)
                                             94

-------
       A similar procedure was used for each lake resulting in a total of 25




    individuals selected per protocol for the purpose of the pilot study. The




    majority of fishermen selected in this manner came from the states of




    Minnesota,  Wisconsin, and Michigan. There were no individuals from the state




    of Illinois since available records indicated that these individuals were




    inland commercial fishermen.




C.  Questionnaire




       The questionnaire developed for this pilot project was designed to assess




    the fish consumption practices, behavioral patternstand health status of




    commercial  fishermen and their families.  There were two   forms of this




    questionnaire; one for the mailed questionnaire format (Protocol III) > an<* one




    for the telephone survey (Protocol I) and  the  telephone  survey with  a




    pre-mailed  set of questions (Protocol II).  In addition,  the questions were




    modified for a proxy interview.  There were no major differences among these




    questionnaires. In some instances the sentence structure has been rearranged




    to accomodate slight differences in subject approach.  (See Appendix II ).




       Specific information requested from commercial fishermen included the




    following topics:




       1.  Demographic  characteristics  (i.e., age, race, sex)




       2.  Occupational histories




       3.  The fishing practices and consumption patterns of study participants




           and  their family members




       4.  The smoking histories of study participants (i.e., cigarette,  cigar,




           and pipe usage)




       5.  The drinking histories of study participants (i.e.,•liquor, wine, and




           beer usage)





                                        95

-------
 6. The prevalence of selected diseases among study participants and their




    family members




 7. The pregnancy histories of female participants and the wives of male




    participants




 8. The prevalence of birth defects among family members of study participants




 9. A participant check list of symptoms relevant to PCB exposure (and other




    organic chemicals e.g., prior exposure to DDT)




10. The menstrual histories of female participants and the wives of males




    participants






D.  Protocol I Procedure




    Twenty-five subjects were randomly allocated to Protocol I by the procedures




 described in the preceding section. A file folder was made for each individual




 and labeled with the individual's name, address, telephone number, and protocol




 number.  Materials mailed to each subject included an introductory letter,  a




 medical consent form, and a stamped,return-addressed envelope to return the




 consent form,  (see Appendix III).




    To facilitate telephone interview scheduling, these materials were mailed




 to five different subjects per day so that by the end of a five-day period all




 twenty-five subjects had been mailed a Protocol I packet.   Subjects were




 telephoned seven days after the individual mailing dates of the study materials




 (see Section G).




    In the event that the materials packet was returned to the University because




 the subject was deceased or the address was incorrect, attempts were made to




 1) locate an appropriate proxy for an interview and retrieve the death certi-




 ficate for the original subject or 2) obtain the correct address for the subject




 and request an interview.  If these procedures failed, the subject was con-




 sidered lost to follow-up and his/her folder was placed in the "complete" file.





                                     96

-------
    At the time of subject contact it was determined whether or not the individual




wished to participate in the study.  The outcomes regarding subject participation




included:




    1) Subject could not be interviewed at the time of the call.




    2) Subject was deceased.




    3) Subject refused to participate in the study.




    4) Subject agreed to be interviewed at the time of the call.




    If the time of the call was inconvenient for the subject a date and time was




established for a return call and the subject's folder was placed in the "pending"




file with the corresponding date for retrieval.




    If the subject was deceased, it was determined whether or not the contacted




individual was appropriate for a proxy interview.  If the contacted individual was




appropriate as a proxy, an interview was requested. If the proxy subject refused,




additional next of kin were contacted for a proxy interview.  If no next of kin'were




available as proxies, the subject's folder was labeled "complete  without an inter-




view" and a request for a death certificate was sent to the appropriate state vital




statistics--off ice.





    If the subject refused to be interviewed his/her folder was placed in an




"initial refusal" file for a three-week waiting period. "Following this period the




subject was called again and asked to participate in the study. If the subject




refused a second request,his/her folder was placed in the "complete" file as a




refusal.



    If the subject agreed to an interview either on the initial or second request




the subject was interviewed.  Following completion of the interview the subject




was asked to sign and return the medical consent form. If the consent form was






                                        97

-------
not returned within a period of ten days, a second letter requesting the




signed medical consent form was mailed to the subject.  If the consent form




was not returned after another ten day period the subject was called to




elicit  his/her cooperation in returning, the consent form. All efforts were




made to obtain the signed medical consent form for study participants;  however,




in those instances where these attempts were  unsuccessful, the subject's




folder was placed in the "complete"file under the "no validation" category.





    Upon completion of the interview and acquisition of a signed medical




consent form, the study subject's folder was turned over to the validation




section for the purpose of verifying  the participant's medical history. A




detailed flow-chart regarding Protocol I procedures appears  in Figure  3






 E.   Protocol II  Procedures




    Protocol II follows a similar format to that of Protocol I (see Figure 31 ,




the only difference being the contents of the mailed packet for Protocol II




which consisted of an introductory letter, a stamped, return-addressed en-




velope, a medical consent form, and,in addition, a list of questions regarding




fish consumption practices, behavioral patterns, and general health (see




Appendix V   .  Furthermore, the mailing of this packet was completed during the




week of telephone contact and interviewing for Protocol I.  This scheduling




 system prevented the development of an interview backlog and facilitated




 the completion of both protocols (see Section G).







 F.   Protocol III Procedures




    Twenty-five  subjects were randomly allocated to Protocol m by the pro-




 cedures described in an earlier section. A file folder was made for each




 individual and labeled with the individual's name, address, telephone number,




 and  protocol number.  A packet consisting of an introductory letter, a medical




                                  98

-------
 S
 r
 oade.
cct raiu
>>col I.
J^ly 'allocated to
A file Colder it
                                                                                             ( 1UUH6   J
                                                                                            rllOTOCOL  I
Aeedical consent  ton and the
introductory  letter *r« cuiltd
to tin lujbect.
Hie mbjecc  (older U placed in
the pending  file dated on* weak
io advance of  filing date.
                   U
                 pacV«t
              returned to
            tht Univeriicyt

                            VtS
                    	JL
                                                      Sulijm  foltlnr ie  ruh"»vct(  (toe
                                                      panJlng Ctl* on th« uppropriatfi
                                                      U«te and tlia ititiject Is eattqj.
                                                                                    TBS"
                                                             iulijret le IntnrvEttweil fliiJ the
                                                             Inlnrvtrw corapUtoJ.   (Proxy
                                                             tntcrvltu noted tf npprapct*lft
                                                             nut] iutij*et folitur li UliotflJ
                                                             duccmed nnd cant to Y«Hd«tlon
                                                             for dcnih CartJflcatc rggueit.)
tltte and U«o eBUfcltihcrf lor »
rcttttn c*lt and subject  loldir
I* f»t«c«d in peiitHnc file "Ith
eorrcipoftdlng d«c« for r*tr|«w*t.
Sulijtct J«
(f contact:
fite for pro
             I
                                                    t returned du» to
                                                     of tuUj«cC*
 5*rntl to tracing  with  foliar frtui
 (h<  pending Ii!«.
    Det*n»|a«  ntst of kin for
    proxy  Interview.
  to r
1
0, . 	 i
f in tol.lir labeltd "Loit
atloy-«y.H

f
Subject J«c«a««dl Mo pro«y
int«r«lcu available. Libel
tubjcel folder "teceaicd" and
«nd 10 validation fcr death
certificate «ei«e»t. •'
                                                                   Qimtluniulrt l< placet! In aul«ji>ci
                                                                   CoMor labeled "Interview «pU«a'
                                                                   end put In pending file d«tv*4 Irn
                                                                   i)n)T« ia advuitr.* of flllnj  called.
                                                                                                       ainaa ta° interview
                                                                                                                                                                   i
                                                                                                                                                        Give «,ve>tlonaelre to toJinj
                                                                                                                                                        Indicating "No Validation."
                                                                                              flace In {elder labelled
                                                                                              "R.lu.ol."

-------
consent form, a stamped,return-addressed envelope, and a questionnaire was




mailed to the subject (see Appendix III).






    In the event that the correspondence packet was returned to the




University because the subject was deceased or the address was incorrect,




attempts were made to  1) locate an appropriate proxy for an interview and




to retrieve the death certificate for the original subject or  2) obtain




the correct address for the subject and mail the individual a Protocol III




packet.  If these procedures failed, the subject was considered lost to




follow-up and his/her folder was placed in the "complete" file.




One month following the initial mailing, those individuals who had not




responded received a second Protocol III packet.  Individuals not responding




to the second mailing were considered refusals and their folder was .placed-in




the "complete" file in  the refusal category.





    Study subjects responding to the initial or second request for partici-




pation were screened to determine if they had returned their medical consent




forms with the questionnaires.  Those individuals who failed to return their




consent forms 'were mailed a second request for the medical consent form. In




those instances where these attempts were unsuccessful, the subject's folder




was placed in the "complete" file under the "no validation" category.






    Upon acquisition of a completed questionnaire and medical consent form the




study subject's folder was turned over  to validation for the purposes of




verifying the participant's medical history. A detailed flow-chart regarding




Protocol III procedures appears in Figure  4.





G.  Timetable for Pilot Project Initiation




    A  timetable was developed for the initiation of subject contact and




interviewing for all three study protocols.





                                  100

-------
             5uli)«?r c r Amlomty  el loc .il eit
             to Protocol t!l.   A f 11-'
             folder ia mdiic.
                          \t>
                                                                                             rtcune  4
                                                                                           PROTOCOL  III
                                                                                                             See validation (low rtiort.
             Subject U ml led » medical
             Consent fora. i,uc»u lonnaiie,
             and c-tvn* letter.
                                                                                                                                                           YKS
             «!T  I'lbjcci  lolOcr it pi need
             itt I lit-  pvn

                                                                                                                                              Subject la Bailed « aecond
                                                                                                                                              rtoueit lor .edicat con.r.lt
                                                                                                                                              loot.
fattens.n« next of kla for
proxy interview.
                                                                                        NO
                                                                                             /
                                                                                                lias
                                                                                                 joct
                                                                                            rec«lv«d two
                                                                                          nllinga of Proto~\
                                                                                         col 111 ajt«ri«U7
                                  Subject tJtce/tBfd.  No proxy
                                  interview avcilcbla.  tobrt
                                  • ubjcec folder d*>ce
-------
    The initial mailing of Protocol III materials took place during the week




of June 1 to 5, 1981.   Materials for five of the twenty-five subjects were




mailed each business day.  All twenty-five subjects were mailed materials by




the end of the five-day period.   After a period of one month, those subjects




not returning their materials were sent a second Protocol III packet during




the period June 29 to July 3, 1981.





    The initial mailing of Protocol I materials took place during the period




June 8 to 12, 1981.  Materials for five of the twenty-five subjects were




mailed each business day.   All twenty-five subjects were mailed materials by




the end of the five day period.  The corresponding phone contacts and interviews




took place seven days following the dates of mailing of Protocol I materials




for each subject.  The contacts and interviews were begun on the. respective




days of June 15 to 19, 1981.





    The procedure for Protocol II was similar to that of Protocol I with the




mailing of Protocol II materials occurring during the period June 15 to 19,




1981 and the contact and interviewing via telephone occurring seven days




following the mailings of Protocol II materials to each study subject. The




schedule in Figure  5 outlines the implementation of the pilot project.





 H.  Validation Procedures






    Following completion of  the interview and the acquisition of the signed




consent  form the subject's folder was sent to the validation section for




purposes of verifying  the medical history of the study participant.





    A  validation form  (see Appendix III) was completed for each subject and




contained information  regarding the name, address, and phone number of the study




participant, the name,address, and phone number of the medical sources, the




date and reason  for contacting medical sources, and a calendar noting the dates





                                 102

-------
                                              FIGURE  5.
                                      PROTOCOL  IMPLEMENTATION
Heek of June 1 to June 5

Initiate Protocol III   (Mailed Questionnaire)

                             6/1/81            6/2/81
                             Monday            Tuesday
Mail materials to:        ,5 subjects         5 subjects

Subject folder placed in pending file for one month

Week of June 8 to June 12
Initiate Protocol I   (Telephone Interview)

                             6/8/81
                             Monday
Mail materials to:         5 subjects

Week of June 15 to June 19

Begin telephone interviews for Protocol I
Initiate Protocol II

                            6/15/81
                            Monday
                       *5 participants
                         #5 subjects

Week of June 22 to June 26
    6/9/81
    Tuesday
   5 subjects
   6/16/81
   Tuesday
5 participants
  5 subjects
                        6/3/81
                       Wednesday
                       5 subjects
   6/10/81
  Wednesday
  5 subjects
   6/17/81
  Wednesday
5 participants
  5 subjects
                      6/4/81
                     Thursday
                    5 subjects
                      6/5/81
                      Friday
                    5 subjects
   6/11/81
   Thursday
  5 subjects
  6/12/81
  Friday
5 subjects
   6/18/81
   Thursday
  6/19/81
  Friday
5 participants   5 participants
  5 subjects       5 subjects
Complete telephone interviews of Protocol I particij* •.•;!..••
Begin telephone interviews of Protocol II participants using similar procedures as outlined above

Week of June 29 to July 3

Begin status check on Protocol III participants and initiate second mailing for those individuals failing
     to respond to the first request
Complete telephone interviews of Protocol I participants failing to interview in the proceeding week
Complete telephone interviews of Protocol II participants
 Telephone Interview of Protocol I Subjects

 Mail Introductory Packages to Protocol II Subjects

-------
and status of request/acquisition of medical records.





    A request for medical records, a photocopy of the medical consent




form, and an abstract form were mailed to all medical sources named by the




study participant (see Appendix III). The subject's folder was placed in the




validation file dated two weeks in advance of the filing date. If the medical




records were not received after a two week period, a second request for medical




records was initiated.  After three non-responses via the postal system,




medical sources were contacted by phone to obtain their cooperation. If these




methods failed to validate study participant's medical histories, his/her




folder was placed in the "complete" file with the validation complications noted




for coding and data analysis.





    In instances where the study subject was deceased, a death certificate




request letter and abstract form were sent to the appropriate state vital




statistics office, (see AppendixHI).  The subject's folder was placed in the




validation file dated two weeks in advance of the filing date. If the death




certificate was not received within a period of two weeks, a second request




was initiated. After three non-responses from the state vital statistics




office, the subject's folder was placed in the "complete" file and the un-




availability of the death certificate was noted.





    Following the successful acquisition of all validation materials medical




histories will be verified and coded for analytical purposes.





    A detailed flow-chart describing the validation procedures appears in




Figure  6,





I.  Tracing Procedures




    Tracing procedures were intitiated in those instances where the study




materials were returned  to the University indicating that the study subject




was no longer located at the address given on the license.





                                  104

-------
                                                                                    Figure   t>
                                                                            Validation Procedures-
O
Ul
                                                                                                                   in «dv4nc* of filing
                                                                t  folder
                                                             .  Ko  Ifcslh
                                                                                                                         itnt to t
                                                                                                            Ub*l*d  "Ho V»l(cUt Un.
                                                                                                                                                      |1a»th crrt t flr^t •
                                                                                                                                                      Iro« •!•!« hcntlh
                                                                                                                                                      with »*om (orb letter
           foldrr vlilcli inclu
             «:M ionna lie and «
             J ic« I content  fon> (tota
                                                                           Medic*!  *o»rc«i
                                                                           «n ^Uon« to alicc co
        co.-aplotcb *
aliJjcion carJ for *tI
       condlt i ani
   tout, Inyurtng
 Sjuci  (olJ.'t £on(»[n* ft
                     fora.
                                                                                                                                                               I..WT subject ta\d«,  "K«
               L«tt«ct fora will be
           nt»  il»ti»l tvo
           • Uv«iic« at tlm
                               In
                                                                        Vtllilclor coepletel »«l »*lt>
                                                                        e tfcand roi|ur«t let uedic&l
                                                                        r«corJ« vlth «b>(V»et fora.

-------
    Initial tracing procedures included the use of directory assistance,




reverse directories (Polks and Coles),  and the post office to inquire about




prior addresses of the study subjects.




    If these procedures failed to locate the subject,  a search request to the



state department of motor vehicles was  initiated.    This procedure either




resulted in a known address for the subject or indicated that the subject




did not hold a driver's license in that state.





    Advanced tracing procedures included contacting crew members  and other




commercial fishermen, contacting local  fishing ports,  contacting  commercial




fishing organizations, requesting information regarding state boat regis-




 trations, and contacting Great Lakes  fish wholesalers.





    If these methods failed to locate  the study subject, he/she was placed




in the "complete" file noting that the  individual  was  lost to follow-up.  A




detailed flow-chart of tracing procedures appears  in Figure  7.
                                  106

-------
1  Fil-s  returned  from post
  of [ice on  current resins-nee
  indicating study subject
  is  no lor.y?r  at the
  address listed.
                                                           FIGURE 7

                                                      TRACING PROCEDURES
     File sent to tracing
Change subject's tile
status to:  "Lost to
Follow-up."
                                                                                                  I
                                                                                                YES
  Initial, iincing:
    tracer utilizes
     1) Directory assistance
     2) Polks and Coles
     3) Post office o£ prior
        addtess
                        YES
                                                 Placo new address in
                                                 "SIR."  File and enter
                                                 subject's status in
                                                 calendar file for
                                                 immediate contact.
                                                                                          Assign folder to a tracer
                                                                                          and log accordingly by date.
                                                                                          Further methods include:
                                                                                            1)  Calls to crew members
                                                                                               listed on state license
                                                                                            2)  Calls to local potts
                                                                                            3)  Calls to local fishing
                                                                                               associations
                                                                                            4)  State boat registrations
                                                                                            5)  Ftesh water fiah
                                                                                               wholesalers
                                                                                                      NO
                                  •YES
      Is
    subject
 located at a
known addiess?
V

O
\
/

Send a search request to
the respective DMV

f
J

\

* i
•iO
1
Subject license returned
to DMV due to subject
acquiring a license in a
new state.

-------
J.  Coding




    This section outlines the coding proposals for the Great Lakes pilot




project questionnaire.  Testing of the coding and data analysis proposals




would involve the use of computer statistical packages to determine an




efficient analytical procedure applicable to a large-scale study.




    The general instructions regarding the Great Lakes pilot study coding




proposal include:




    1)  placing a "9" in all coding spaces for a response of "dont know"




    (e.g., Hi).




    2)  placing an "8" in all coding spaces for a response of "refused




        to answer" or "not answered" (e.g., _8 £ 8).




    3)  placing a "0" in a coding.space when an answer requires less coding




        space than allotted.  The coding spaces to the left would be filled




        with zeros (e.g., three spaces provided and the answer given is




        9 = £ £ 9) •



    The proposed set of coding instructions rOr the pilot study questionnaire




is presented in Appendix IV.
                                   108

-------
K.  Data Analysis




    This, section outlines the data analysis proposal for the Great Lakes




pilot project.  This proposal in conjunction with the coding proposal




outlined in the preceeding section should be tested prior to implementation




of a large-scale study.  Testing would involve the use of computer




statistical packages to determine an efficient analytical procedure




applicable to a large-scale study.




     Participant demographics will be used to develop a descriptive




profile of this occupational cohort.  Fishing histories (i.e., the number




of years fished and location where participant fished most often) will be




evaluated in conjunction with reported fish consumption patterns to deter-




mine any discrepancies between these categories.  That is, responses in one




category can be used to verify the responses in the other.  In addition,




these responses will be used to determine the number of individuals with




the greatest potential PCB/organic contaminant exposure.  Based on the




results of this evaluation, study subjects will be stratified into four




exposure groups.  High, moderate, low, and none.  Individuals classified




as "no exposure" will be used as an internal control group.




     The information obtained from the survey regarding morbidity/mortality




within these four categories of exposure will be used to determine fre-




quencies.  These frequencies will be used to calculate an odds ratio to




determine potential health differences among the four exposure categories.




     In addition, these analyses will be performed for family members  (i.e.,




spouse and children) when their exposure (i.e., fish consumption patterns)




are known to be similar to that of the study subject. . Separate odds ratios




will be calculated for stud}1- subjects in each exposure group to evaluate  the




potential confounding effects of smoking and drinking behavior on health.






                                   109

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    The frequencies of birth defects and pregnancy complications among




family members and spouses (wives) of the study participants will be evaluated.




Exposure in these individuals will be based on two criteria including 1) the




exposure category of the study subject and  2) the similarities of fish con-




sumption practices between study subjects and their family members.  These




frequencies will be used to calculate an odds ratio to determine potential re-




productive dysfunctions among the exposure categories.




    Menstrual histories of female participants and the spouse of male




participants were requested and these individuals will be stratified according




to their potential exposure.  Menstrual dysfunction frequencies will be




calculated for each exposure category and used to calculate odds ratios.




    The hypothesis that long histories of fish consumption (especially fish




taken from areas of known environmental contamination) may increase PCB/organic




contaminants exposure and result in demonstrable human health effects is not a




realistic outcome of this pilot study.  The purpose of the analytical section




of this pilot study is to test the feasibility of these proposals.  Appendix V



contains a proposed set of analytical procedures and methods for the pilot




study questionnaire.
                                110

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IV.  Pilot Study Results




     A.  Protocol I





             The objective of the protocol I procedure was to evaluate the




         effectiveness of a telephone interview format among a cohort .of commercial




         fishermen.  A correspondence packet containing a medical consent form, an




         introductory letter, and a stamped, return-addressed envelope was mailed to




         the study subject.  After seven days the subject was contacted by phone and




         and interview was requested, (see Section III-D).





             Twenty individuals or 80% of the protocol I cohort were contacted by




         phone and eighteen or 90% of these individuals consented to an interview.




         Four subjects were never home at the time of the call and repeated efforts




         to contact these individuals failed.  One subject had an unpublished phone




         number and efforts to contact this individual through the mail failed to




         elicit a- response.




             The current status of protocol I participants is summarized in Table 25.




         Of the eighteen or 72% of the total cohort who granted an interview, four




         have failed to return their signed medical consent form and one has refused




         to return the signed medical consent form. Efforts are continuing to  1)




         obtain the consent forms of the four subjects (29, 39, 45, and 46) who have




         not returned their signed consent form or  2) obtain a response indicating a




         refusal to cooperate with this aspect of the study procedure. Thirteen




         individuals or 52% of the total cohort are complete or partially complete for




         validation procedures.





             The average time for an interview was approximately thirty-five minutes




         with a range of approximately twenty to fifty minutes.  In addition, no  study





                                        111

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subject
 study
number
  26
  27
  28
  29
  30
  31
  32
  33
  34
  35
  36
  37
  38
  39
  40
  41
  42
  43
  44
  45

  46
  47
  48
  49
  50
                                 TABLE 25
                       Status of Protocol I Participants
                               subject
                               status
interview complete - validation procedures are partially complete
interview complete - validation procedures are complete
subject not home at time of call
interview complete - signed consent form has not been returned
refusal
interview complete - validation procedures are complete
subject nothome at time of call
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
subject not home at time of call
interview complete - validation procedures are complete
interview complete - validation procedures are complete
interview complete - signed consent form has not been returned
interview complete - validation procedures are partially complete
unpublished phone number
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - signed consent form has not been returned for
                     wife (subject divorced)
interview complete - signed consent form has not been returned
interview complete - subject refuses to return signed consent form
interview complete - validation procedures are complete
refusal
subject not home at time of call
                                    112

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materials were returned due to  1) an incorrect mailing address or  2) the




death of the study participant.  This suggests that information regarding commer-




cial fishermen obtained from state files, publication lists, and fishing




organizations was accurate with respect to address and reflected a living cohort.





    Several problems were encountered with the telephone interview format in-




cluding:




    1)  scheduling interviews with the study subject and spouse




    2)  subjects who were never at home at the time of call




    3)  subjects who were without a phone




    4)  subjects who had unlisted phone numbers





    Despite these difficulties the addresses and living status were verified




for all protocol I participants.





    In those instances where interview times were difficult to obtain, either




the subject or  the  spouse was requested to complete the entire interview.




This situation occurred in three of the eighteen respondents. In 'one instance,




the study subject completed the entire interview and in two instances the spouses




of the study subject completed the entire interview.  In eleven of eighteen




respondents both the subject and spouse participated in the interview.  Four of




the eighteen respondents were living alone (i.e.,  single, widowed, separated/




divorced) and the questions regarding reproductive histories and dysfunctions




were not applicable.



    In those instances where subjects did not have a phone or had an unlisted




phone number, a letter was sent requesting their participation along  with a card




requesting a number where they could be reached.  Of the two individuals which fit




this category one responded and granted an interview.





    Four study subjects were never home at the time of call despite consistent




efforts to reach them.  A possible explanation for their absence could be that




                                  113

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     they  are  living on  their  fishing vessel during the seasonal months. This




     suggests  that a large  scale study should be undertaken during  the off-season




     when  fishermen are  more likely to spend time at their listed residence.





        In  general, the study subjects in protocol I responded well when asked  to




     participate in the  survey.  There appeared to be no difficulties in interpreting




     the survey questions on the part of  the study subjects with the exception of




     questions 149 and 150.  These questions ask for the average length of  the




     menstrual cycle and the average length of the "bleeding"  period, and were




     consistently misinterpreted by respondents despite explanations by the inter-




     viewer.   In addition,  a potential problem may exist with  the validity  of




     question  26  regarding the pounds of  fish consumed in a year.   Many respondents




     hesitated when answering  this question and needed to be coaxed into providing




     an answer.  However, this answer will be cross-checked with the responses to




     questions 19 and  22 regarding the number of fish meals consumed per week or per




     month to  provide  a  valid  fish consumption index for data  analysis purposes.








B.   Protocol  II




         The objective of the  protocol II procedure was to evaluate the effectiveness




     of a telephone  interview  preceded by a mailed set of questions identical to




     those asked  during  the telephone survey.  A correspondence packet containing




     a letter  of  introduction, a medical  consent form, a stamped, return-addressed




     envelope  and a  set  of questions identical to those on the questionnaire was




     mailed to the  study subject.  After  seven days the subject was contacted by




     phone and an interview was requested.(see Section III-E).





         The purpose  of  the mailed  set of questions identical  to those asked in  the




     interview was  to  evaluate whether  this protocol produced  more  complete and




     valid responses  on  the part of  the  study subject given the fact that  they would




     familiarize   themselves with  the questions prior to the interview.




                                       114

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    Twenty-two individuals Or 88% of the protocol II cohort were contacted by




phone and 19 or 86% of these individuals consented, to an interview.  Two sub-




jects had unpublished phone numbers and efforts to contact these individuals




faile'd to elicit a response.  One individual had recently moved to California and




had not established a new residence at the time of this study.





    The current status of protocol II participants is summarized in Table 26




Of the 19 or 76% of the total cohort who granted an interview five have failed to




return their signed medical consent form and one has refused to return the signed




medical consent form. Efforts are continuing to  1) obtain the consent forms.of the




five subjects (51, 57, 59, 63 and 75) who have not returned their signed con-




sent forms or  2) obtain a response indicating a refusal to cooperate with




this aspect of the study procedure.  Twelve or 48% of the total cohort are




complete or partially, complete to validation procedures.




    No study materials were returned due to  1) an incorrect mailing address




or  2) the death of the study participant;  In addition, the addresses and




living status were verified for all protocol II subjects with the exception




of subject 70 who was in transit to an out of state residence at the time of




the study. In those instances where interview times were difficult to obtain,




either the subject or their spouse was requested to complete the entire inter-




view.  This situation occurred in eight of the nineteen respondents. In five




instances the subject completed the entire interview and in three instances




the wife completed the entire interview.  In seven of nineteen respondents




both the subject and spouse participated in the interview.  Three of nineteen




respondents were single (i.e., never married) and the questions regarding




reproductive histories and dysfunctions were not applicable.  One respondent




refused to let their spouse be interviewed, and as a result, no information was




obtained regarding reproductive histories and dysfunctions for this subject:.





                                 115

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                                 TABLE 26

                      Scatus of Protocol II Participants
..subject
 study
number
  51
  52
  53
  54
  55
  56
  57
  58
  59
  60
  61
  62
  63
  64
  65
  66
  67
  68
  69
  70
  71
  72
  73
  74
  75
                               .subject
                               status
interview complete - signed consent form has not been returned
interview complete - validation procedures are complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are complete
refusal
interview complete - validation procedures are partially complete
interview complete - signed consent form has not been returned
refusal
interview complete - signed consent form has not been returned
refusal
interview complete - validation procedures are partially complete
unpublished phone number
interview complete - signed consent form has not been returned
interview complete - subject refuses to return .signed consent form
unpublished phone number
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
directory assistance does not have a listing
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - validation procedures are partially complete
interview complete - signed consent form has not been returned
                                     116

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         The results and problems experienced in protocol II are similar  to




     those experienced and discussed in protocol I.   Methods used to  remedy  these




     problem areas were identical.  The major difference between these  protocols




     was the potential impact of the pre-mailed set  of questions in protocol II




     which was to aid in the subject's development of responses  prior to  an  inter-




     view on the telephone.  The effectiveness of this protocol,  as compared to




     protocol I, will be thoroughly evaluated following the completion  of the vali-




     dation procedures.  However, several potential  problem areas were  identified




     including:




         1)  the number of questions was too large




         2)  the specificity of the questions may have been offensive to  several




             participants




         3)  the study subjects did not use the set  of questions for  the  purposes




             intended




         In general, the study subjects in protocol  II responded well to  the




     survey questionnaire. In addition,  family members (i.e.,  spouses)  responded well




     when asked to participate in the survey.  Similar problems  with  questions 26,




     149, and 150 were encountered among the protocol II participants.  Future




     application of this protocol to a cohort of commercial fishermen should con-




     sider modification of the pre-mailed set of questions. Both a reduction in  the




     number of questions and the specificity of those questions  appear  warranted.




     However, the comparison of validation results for protocols I and  II may alter




     this observation.






C.   Protocol III




         The objective of the protocol III procedure was to evaluate  the  effective-




     ness of a mailed questionnaire format among a cohort of commercial fishermen.




     A correspondence packet containing a medical -consent form,  a stamped,  return-




                                       117

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addressed envelope, an introductory letter, and a questionnaire was mailed to
the subject. A. second, identical packet was mailed in the event that the study
materials were not returned following a one month time period, (see Section
III-F).

    Six individuals responded to the initial mailing for a response rate of
24%.  Of these six individuals, one failed to return the signed medical records
request form.

    An additional five individuals or 20% responded to the second mailing. Of
these five individuals one returned a refusal message, one responded saying
that he was not nor had he ever been a commercial fisherman, and two failed co
return the signed medical request consent form.

    Fourteen individuals or 56% of the study subjects failed to respond in
any manner to the mailed questionnaire format presented in section III-F.
    The current status of protocol III participants is summarized in Table 27
Of the eleven individuals or 44% who responded to the mailed questionnaire

format seven or 28% are complete or partially complete for validation procedures
Two or 8% or complete without consent forms,  and efforts are continuing to
1) obtain the consent forms of subjects 3 and 17 or 2) obtain a response indi-
cating a refusal to cooperate with this aspect of the study procedure.
    The response time for the return.of the study materials for protocol III
ranged between twelve to fifteen days for both mailings. These results indicate
that  the one month waiting period was an appropriate length of time for parti-
cipant response.  In addition, only one study packet was returned due to an
incorrect mailing address and subsequent efforts to obtain the correct address
were  successful. No study materials were returned due to the death of a study
participant.  This suggests that information -regarding commercial fishermen ob-
tained from state files, publication lists, and fishing organizations was

                                   118

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                                 TABLE 27
                       Status of Protocol III Participants
subject
 study
number
   1
   2

   3

   4

   5
   6
   7
   8
   9
  10
  11
  12
  13
  14
  15
  16
  17

  18

  19

  20
  21
  22
  23
  24
  25
                               subject
                               status
No response following two mailings
responded on the second mailing indicating that he was never a
commercial fisherman
responded on the second mailing - signed consent form has not
been returned
responded on the second mailing - validation procedures are
partially complete
No response following two mailings
No response following two mailings
responded on the first mailing - validation procedures are complete
responded on the second mailing - refusal
responded on the first mailing - validation procedures are complete
No response following two mailings
No response following two mailings
No response following two mailings
responded on the first mailing- - validation procedures are complete
No: response following two mailings
No response following two mailings
No response.following two mailings
responded on the first mailing - signed consent form has not
been'returned
responded on the first mailing - validation procedures are
partially complete
responded on the second mailing - validation procedures are
partially complete
No response following two mailings
No response following two mailings
No response following two mailings
No response following two mailings
responded on the first mailing - validation procedures are complete
No response following two mailings
                                      119

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accurate with respect to address and reflected a living cohort.




    A major problem experienced with the mailed questionnaire format was




the incorrect or incomplete responses obtained from five of the  nine study




subjects who signed and returned the questionnaire. It is difficult to assess




whether this was attributable to questionnaire length, questionnaire mis-




interpretation, or individual inconsistency.  However, four individuals com-




pleted the questionnaire in its proper context suggesting that the difficul-




ties with incomplete responses are most likely due to the length of the




questionnaire and/or inconsistency on the part of the study subject.




    In general, the participants correctly completed the questions regarding




fishing practices and consumption patterns and their behavioral patterns (i.e.,




smoking, drinking).  Incomplete or incorrect responses were most often en-




countered with the questions regarding familial medical histories.




     Future application of this protocol procedure to a larger cohort should




consider several modifications including:




     1)  a reduction of the waiting period for a participant response from




         one month to two to three weeks,




     2)  structural changes of the questionnaire to facilitate participant




         response.




     3)  telephone contact with study subject to encourage cooperation with




         the survey in the event that two mailings failed to elicit a response.




D.  Discussion




     A significant factor regarding the appropriateness of a commercial
                                  120

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fishermen cohort to assess the potential health impacts of organic




contaminants in the environment is the distribution of fish consumption,




and thus potential exposure to organic contaminants, among this population.




     A preliminary analysis was performed based upon the combined responses




of protocols I and II to evaluate the number of fish meals per months, the




number of years consuming fish with this frequency, the number of pounds




of fish consumed per year, and the types of fish most often consumed.




Table 28 summarizes these preliminary results.




     In general, the combined responses of individuals from protocols I




and II suggest that commercial fishermen eat fairly large quantities of




fish and, for the most part, have been doing so for a good many years.




Approximately 14% of the respondents consume between 0-3 fish meals per




month, 42% consume between 4-7 fish meals per month and 44% consume  > 8




fish meals per month.  The fish species most frequently mentioned as being




consumed were perch, followed by lake whifefish, lake herring, and lake




trout.




    It is interesting to note the distribution of study subjects from




protocols I and II regarding the pounds of fish consumed per year; the




majority of which report  £.50 Ibs/year.  These results appear to be in-




consistent with the results regarding the number of fish meals consumed




per month.  A possible explanation is that some individuals consume large




quantities (i.e., >2 Ibs) of fish at one sitting compared to other in-




dividuals who consume small quantities (i.e., < *slb).  To resolve this




potential inconsistency an additional question regarding the average




amount of fish consumed per meal should be considered for inclusion in a




large-scale study.




     These preliminary results suggest that there is a good distribution






                                  121

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                                                                   TABLE 28
                                             PHELiHIHMIY nesuyrs ron PROTOCOLS  i MID_II
Distribution o<  study
subjects frow Protocols
1 and II regarding  the
number of flflh feeala
eonauaed per. eontn
Distribution of  study
subjecte Iron Protocols
I and II regarding  the
number of yeara  ffah
uero consuued with  this
frequency
Distribution of  etuoy
aubjeete ftos Protocols
I and II regarding  the
nunber of pounds of  fish
conauoicd per year
Typed of floh eott
often mentioned ae
consumed by respondents
frou Protocols I and  It

mmber of Huh nuetber of
eeele per aonth study subjects
0-1 3
2-3 2
4-i 12
6-7 3
B-« J
10 12




niraber of years
fish conaitneii with number of
thia frequency atudy subjects
0-J 3
6-10 2
11-1) 1
16-20 3
11-30 7
31 21




nu«ber of pounds
of flali consumed number of
per year study subjects
0-2) 10
26-50 «
51-7S &
76-100 3
101 S







fish species
Perch
Lake- uhlttf Uh
Luke herring
Lake trout
Roughflsh
Sxelt
Salmon, Valleys
Chubs
Northern Plk«.
Burbot. Pickerel

*nuiaber of
study 
-------
of fish consumers among this cohort ranging from 0-1 meals per month



to acre than 10 meals per month.  In addition, there is a good distribution




of fish consumers based on yearly consumption amounts ranging from less



than 25 Ibs/year to greater than 100 Ibs/year.



     Humphrey and his associates measured the PCB levels in persons



consuming sport fish and reported that the most frequently recorded



quantity of fish actually consumed was in the 25-35 pounds per year



range.  However, the number of.-participants reporting a consumption



greater than 35 pounds per year was low (52).  Approximately 252 of



Humphrey's cohort consumed  £30 pounds of fish per year.  A comparison



with preliminary pilot results indicates that approximately 50Z of the



commercial fishermen cohort consume  > 50 pounds of fish per year.




These findings suggest that a population sub-group consuming considerably



greater quantities of fish can be identified and are willing.to par-




ticipate in an epidemiologic investigation.
                                  123

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I.  Cost Analysis for Protocol Implementation

    A.  Protocol I

      The cost per protocol I participant can be approximated by determining

the cost of individual study instruments and procedures. The following is a

list  with approximate costs of study materials and procedures used in protocol I.

                letter or introduction               $  .05

                medical consent form                    .05

                return-addressed, stamped envelope      .23

                mailing envelope and postage            . 23

                long distance phone call(interview)
                        $.38 x 35 minutes             13.50

                second request for consent form         .05

                medical consent form                    .05

                return-addressed,stamped envelope       .23

                mailing envelope and postage            .23
                                         Total       $14.62
     Based upon the price of a long distance phone call to New York for an

average interview time of thirty-five minutes the estimated phone costs are

$13.50 per study subject.  In addition, the costs of mailing both an introductory

study packet as well as a second request for medical consent forms are included.

(On  the average there was one additional request for the medical consent form per

study subject).  The total cost for interviewing and obtaining returned study

materials from protocol I participants is approximately $15.00 per subject.

     Not included in this approximation are secretarial and interviewer costs.

Considering these additional expenses the cose per protocol I participant is

approximately $20.00.  However, it must be kept in mind that these are estimated

                                       124

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costs based on average participant's response times. In general, telephone costs

(the major expense of this protocol) will vary according to subject residence and

length of interview time.



B.  Protocol  II

     The cost per protocol II participant can be approximated by determining the

cost of individual study instruments and procedures. The following is a list with

approximate costs of study materials and procedures used in protocol II.


                letter of introduction                   $  .05

                medical consent form                        .05

                a set of questions (5 pages)                .25

                return-addressed, stamped envelope          .23

                mailing envelope and postage                .72

                long distance phone call
                         $.38 x 35 minutes                13.50

               .second request for consent form             .05

                medical consent form                        .05

                return-addressed, stamped envelope          .23

                mailing envelope and postage                .23
                                            Total        $15.36
     The expense figures for protocol II are similar to those of protocol I.

However, the mailing costs, the cost for an enclosed set of questions, and a slight

increase in secretarial costs increase the overall cost per protocol II  partici-

pant.  Considering these additional expenses the cost per protocol II participant

is approximately $22.00.  The variables associated with this cost approximation

are similar to those discussed for protocol I.


                                      125

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 C.  Protocol III




     The cost per protocol III participant can be approximated by determining




the cost of individual study instruments and procedures.  The following is a list




with approximate costs of study materials used in protocol III.





                letter of introduction                   $  .05




                medical consent form                        .05




                questionnaire (23 pages)                   1.15




                return-addressed, stamped envelope         1.45




                mailing envelope and postage               1.62




                second request for consent form             .05




                medical consent form                        .05




                return-addressed, stamped envelope          .23




                mailing envelope and postage                .23
                                              Total      $ 4.88
     The cost of a second mailing to 76% of the protocol III subjects averages out




to be approximately $3.28 per participant. la addition, there was an average of




one additional request for the medical consent form per study subject.




     The cost for the mailed questionnaire format is approximately $8.00 per study




subject.  Considering additional secretarial expenses, involving increases in time




and typing, the total costs are approximately. $10.00 per study subject.




     In summary, the costs associated with protocols I and II are very similar




(approximately $20.00 per study subject) with, protocol II being slightly more




expensive.  The cost associated with protocol III is cheaper by about half. However,




it appears  that both protocols I and II are more cost-effective in that they achieve




a much higher rate of response than protocol III. In addition, the information




obtained by protocols I and II is much more complete than that obtained in protocol




III.  These results suggest that the use of either protocol I or II would be more




                                       126

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cost-effective in a large-scale study and less likely to be biased by non—response

and incomplete information.

     The cost-effectiveness of the added expense of protocol II will be determined

following validation.  If a significant percentage of responses obtained from

protocol II participants are more accurate than those obtained from protocol I, then

the added expense of protocol II procedures may be warranted in a larger study.

Evaluation of the accuracy of responses from protocols I and II, as determined through

preliminary validation, indicate that these differences are slight.  Therefore, pro-

tocol I appears to be the most efficient and cost-effective.

     The costs associated with validation and tracing procedures are the same re-

gardless of the protocol and are discussed in the following section.


D.  Validation

     The cost per study participant for validating medical histories is based upon

the following list of study instruments.

                a letter requesting medical records        $.05

                a copy of the signed consent form           .05

                a medical records abstracting form
                                         (4 pages)          .20

                a return-addressed, stamped envelope        .40

                a mailing envelope and postage              .23

     On the average, two requests were sent to each medical source.  In addition,

the cost associated with obtaining the addresses of medical sources involved an

average of three long-distance calls per source given by the study subject.  These

include.:

                1 call to directory assistance for the phone number

                1 call to a related medical source for information $2.07

                1 call to actual medical source for an address $l-r-31


                                       127

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     There was an average of three medical sources contacted per study subject.




As a result the cost associated with the validation of the medical histories is




approximately $16.00 per study subject.




     Not included in this approximation are secretarial and tracer costs. Con-




sidering these additional expenses, (using an average of one hour to obtain the




mailing address of the medical sources mentioned by the study subjects), the total




cost is approximately $21.00 per study subject.








E.  Tracing




     The cost associated with tracing individuals was minimum in this pilot study




since most of the addresses were current.  In several instances, phone calls were




made to verify the addresses of those individuals with unlisted phone numbers, no




phones, or who were not at home at the time of the call. This cost amounted to an




average of approximately $1.00 per study subject.




     The cost associated with tracing medical sources (i.e., deceased physicians)




is included in the validation expenses per study subject.
                                      128

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                                    Conclusions






     A pilot study was initiated to determine the feasibility of an epidemiologic




investigation, among commercial fishermen, of the association of PCBs on health.




Commercial fishermen were selected as a potential "high risk" population because




of their fish consumption habits and the availability of licensing records from




which a cohort could be extracted. The purpose of this pilot study was to evaluate




three research protocols to determine their effectiveness as epidemiologic tools




and the appropriateness of commercial fishermen as a cohort.




     The pilot study has provided the following conclusions:




     1.  The addresses of all study subjects in Protocols I and II were verified.




         The format of Protocol III was structured such that the verification of




         subject location was not applicable.




     2.  The response rates  for Protocols I and II were similar (72% and 76%




         of the total cohort respectively).




     3.  The response rate  for Protocol III was 44% after two mailings.




     4.  Protocols I and II were more effective in producing:




         a) answers in the correct format,




         b) the highest response rates, and




         c) the most accurate information.




     5.  The differences between Protocols I and II regarding accuracy of infor-




         mation are slight.  Therefore, Protocol I appears to be the most efficient




         and cost-effective of the three protocols tested.




     6.  The fish consumption patterns among this cohort, obtained by compiling




         the information from Protocols I and II, indicates several trends.




         These include:




         a) a good distribution of fish consumption per month among the cohort,




         b) a good distribution of the number of years fish have been consumed




            with this frequency among the cohort,





                                       129

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         c)  a good distribution of the quantity of fish consumed per  year  among




            the cohort,  and




         d)  the preferred consumption of several target fish species from the Great




            Lakes among this cohort.





     These results suggest that commercial fishermen were appropriate as a study




cohort and that Protocol I would be the most effective and cost-efficient method




of epidemiological ascertainment.




     In addition to the pilot study an analysis of the morbidity and mortality patterns




of the Great Lakes populations was conducted.  State vital statistics regarding




county fetal, neonatal, and infant death rates and congenital anomaly rates were




examined for the states of Minnesota, Wisconsin, Illinois, Indiana, Michigan, and




Ohio for every fifth year from 1950 to 1975 and the year 1977.  Furthermore, an




evaluation of the county site, race,  sex, and age-adjusted cancer mortality rates




from the National Cancer Institute's publication "U.S. Cancer Mortality: 1950 - 1969"




was conducted  (80).




     These analyses have generated the following conclusions:




     1.  There were no significant trends regarding the percent differences for




         neonatal death rates and fetal death rates among "lake bordering " and




         'non-lake bordering" counties having rates ^L 1 standard deviation higher




          than  their respective state means.




     2.   There was a slight trend regarding the difference between percent of




          live births with congenital anomalies among "lake bordering" and "non-lake




          bordering" counties having rates _2l 1 standard deviation higher  than their




          respective state means. This trend favored the "non-lake bordering"




          counties.




      3.   The fertility rates for the eight states were not analyzed due to incon-




          sistent reporting between states.




                                       130

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     4.  An evaluation of the cancer rates of the counties of the Great Lakes




         states, stratified according to proximity to the lake,  indicated an




         increasing trend with proximity to the Lakes for esophageal and stomach




         cancers. These trends are still apparent after counties with population




         centers 2t 100,000 have been excluded from the analysis.





     These results suggest that "Lake-bordering"'populations (i.e., white populations)




experience higher rates of mortality due to stomach and esophageal cancers as com-




pared to 'hon-lake bordering1'counties. This trend is consistent when the potential




confounding factor of large urban centers is removed.




     There were no apparent trends regarding the fetal death rate, the neonatal death




rate, and the percent of live births with congenital anomalies,  among "lake-bordering"




and "non-lake bordering" counties.
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          Methodological Approaches for Continued Research






     The purpose of this study has been to determine whether it is




feasible to ascertain analytically any adverse human health effects from




organic contaminants released into the Great Lakes basin.  The results




of the feasibility study indicate-that current epidemiologic method-




ologies are available for detection of human pathologic responses to




organic chemicals.  This conclusion is based upon the following findings:




     1.  Federal and State agencies responsible for monitoring environ-




         mental indices have reported detectable levels of organic




         contaminants in the ambient air, waters, soils, lake sediments,




         and fish speciea of the Great Lakes basin.




     2.  Animal research studies have demonstrated that organic chemicals




         are fat soluble and bioaccumulate in aquatic food chains.  An-




         imal dietary studies have shown that ingestion of contaminated




         foodstuffs may produce a variety of pathologic states.




     3.  Human epidemiologic studies of acute toxic exposures to organic




         chemicals have reinforced the findings of animal dietary studies.




     4.  Existing data sets Ce-g., the Public Health Service publication




         0 80 ) titled:  U.S. Cancer Mortality by County:  1950-1969)




         may be utilized to define patterns of morbidity and mortality




         experienced by human-populations of the Great Lakes basin.




     5.  The Great Lakes Study Pilot Project on Commercial Fishermen




         has data indicating that this occupational cohort consumes




         target fish, species which have been implicated as providing




         an exposure potential to consumers.  Furthermore, some fisher-




         men have reported the consumption of large quantities of




          target species and may constitute a "high-risk" population for





                                132

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         disease.




     Therefore, full scale studies are warranted in addressing the




issues of whether the organic contaminants released into the Great Lakes




basin are causing a demonstrable adverse human health effect.  To discern




the possible complex "agent-host-environment" interactions involved in




this process, multiple approaches will be necessary utilizing the ana-




lytical techniques from many disciplines.  The methodologies utilized in




the feasibility study have been incorporated in the following multi-




faceted protocol:




Analysis of the 1950-1969 County Cancer Mortality Rates:




     The purpose of refining the evaluation of the county 1950-1969




cancer mortality data set ( 80 ) is to identify t^e variables respon-




sible for the discrepancies noted in analysis five.  The procedures to




be added to analysis five are as follows:




     1.  The standard errors for each'me'an age-adjusted site-race-sex




         specific cancer mortality rate would be calculated for the




         three Orders.




     2.  Confidence intervals would be examined comparing the rates




         of Order 1 against Orders 2 and 3.




     3.  For those Order 1 sites whose mean age-adjusted site-race-sex




         specific cancer mortality rate confidence intervals lie outside




         the corresponding confidence intervals for the other Orders,




         data will be gathered on those factors which may be considered




         as confounding variables.  Demographic information acquired




         may include mean income for each county and the percentage




         representation by race, religion, industry, and agriculture.




         Additional data will include the types of occupations located





                                133

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        within a  county which may provide exposure to other agents




        suspected to  cause the disease under consideration.  Statistical




        techniques (e.g.,  regression analysis) will be implemented  to




        discern the influence each possible confounding variable may




        have on the observed differences between rates.




     4.  Environmental monitoring has indicated that the Great Lakes




        vary in the degree to which they are contaminated by organic




        chemicals. A second analysis would be conducted which strati-




        fied the  counties  by lake.  The purpose of this second




        evaluation would be to determine if the trends noted for the




        Order 1 counties were due to a subset of Order 1 counties




        associated with a  particular lake.




     5.  In analysis five all counties which were not of the first or




        second Order  were  considered third Order counties.  In r.ha re-




        analysis  only those counties which are geographically .adjacent




         to the second Order counties, and not of 'the first Order,




        would be considered third Order counties.




     The incorporation of these revisions into the protocol for analysis




five would provide two benefits.  First, it would aid in defining those




factors which have produced the differences noted in cancer mortality




among the  counties.  Secondly, the utility of a data bank such as the




county 1950-1969 cancer mortality data  (80 ) will be evaluated for its




usefulness in generating testable hypotheses regarding disease etiology.






A Comparative Morbidity/Mortality Survey of Great Lakes Counties with




Varied Levels of Organic Contamination:




     Two Great Lakes counties would  be  selected for comprehensive reviews




of environmental and human health parameters.  One county known from





                                 134

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state and federal monitoring programs to be extensively contaminated




by organic compounds would be selected for comparison to a county known




to have minimal contamination.  The counties would be comparable on the




demographic variables listed below.




     Upon selection of the study counties, thorough environmental char-




acterizations would be initiated.  The following 'information would be




evaluated:




     1.  The data files of the regional Environmental Protection Agency




         (EPA) offices would be reviewed regarding surface water dis-




         chargers.  The drinking water sources would be assessed in




         terms of location and mineral analysis.




     2.  Environmental evaluations would be conducted on ambient waters,




         sediments, ambient air, and landfills.




     3.  A market basket analysis would be requested from the Department




         of Public Health on foodstuffs consumed in the communities.




     4.  Industries and agricultures would be reviewed in terms of the




         types of health risks posed to the community and their employees.




     The human health parameters to be reviewed by county include:




     1.  age and cause-specific death rates




     2.  infant, neonatal, and fetal death rates




     3.  percent of live births with congenital anomalies




     4.  birth rates and fertility ratios




     5.  percent of hospital admissions and out-patient services by




         diagnostic category




     6.  medical care utilization rates




     Demographic data would be obtained for both counties regarding:




     1.  population distribution by age, race, and sex





                                135

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     2.   average income and education




     3.   percent of the population employed in various occupations




     4.   the percent of inhabitants living in urban and rural areas




     5.   population densities




     The data on human health parameters will be examined for disparities




between the two counties.  If the difference in rates cannot be explained




by known, risk factors or confounding variables, the routes of exposure




for possible etiologic agents will be discerned.




     This investigation would require a field staff for record abstract-




ing and patient interviews.  Technicians would be required for additional




environmental sampling and laboratory analysis.  The justification for




this study is two-fold.  First, the routes of agent-host-environment




interaction need to be identified.  Second, it could allow a statement




as to whether existing levels of organic contaminants are associated




with adverse health effects in the populations studied.




Health Survey of Great Lakes Fishermen




     The pilot study of Great Lakes commercial fishermen has provided




evidence that some fishermen eat large quantities of fish species known




to have levels of organic contaminants which may exceed Food and Drug




Administration  (FDA) limits.  The  pilot project has developed and pre-




tested material which can be utilized in large scale studies.




     The fishermen's health study  would be implemented in two phases.




The first phase would focus on interviewing all currently licensed




commercial fishermen in  the Great  Lakes  (U.S.  license holders only).




The names and addresses  of the registered  commercial fishermen would




be obtained  from licensing offices of state governments.  The second




component of the study would be undertaken with the cooperation of the






                               136

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Wisconsin Department of Natural Resources (DNR).  Preliminary discussions

have been held with the DNR for a joint survey of Wisconsin sport fish-

ermen.  In 1982 a stratified* one-percent random sample of licensed

sport fishermen would be drawn from DNR files.  Each individual would

be mailed a postcard which has a series of questions on fishing practices

and fish consumption patterns.  Upon return of the completed postcard,

a second stratified random sample will be initiated based upon levels of

fish consumption.  Those sport fishermen selected in the second sample

and all licensed commercial fishermen will comprise the cohort for the

"Health Study of Great Lakes Fishermen."

     Survey interviewers would obtain the fishermen's phone numbers from

"directory assistance" and call to establish a telephone interview.  The

methods for tracing, subject contact, interviewing, validation, and

data analysis have been outlined in protocol I of the Great Lakes com-

mercial Fishermen Pilot Project.

     A subset of the study cohort will be chosen for a survey on dietary

habits and practices.  Project staff will conduct in-person dietary

interviews with the families of fishermen.  Fish samples will be taken

for laboratory analysis.  A seven day dietary record form will be given

to family members to complete and return to the study office.  This

phase of the study is necessary to document potential exposure to organ-

ic contaminants and as a validity check on the questionnaire.  It is

understood that the present documentation of the ingestion of contaminated

fish does not assume that fish eaten at other times were contaminated.

Current clinical effects such as a cancer would require exposure to the

etiologic agent prior to the sampling.

*Stratification would be based on the type of sport fishing license
 purchased in 1982.


                                137

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     The data acquired from the "Health Study of Great Lakes Fishermen"




will address the following issues:




     1.  Is the fish consumed by Great Lakes fishermen contaminated by




         organic chemicals?  If so, do the levels of contamination lie




         within F.D.A. limits?




     2.  Are the methods of fish preparation (i.e., broiling or smoking)




         contributing to the level of contaminants in the fish consumed?




     3.  Is the information reported in the pilot study questionnaire




         consistent with other dietary instruments?




     4.  Are individuals who consume large quantities of fish at a




         greater risk of disease than low or non-consumers?  If so,




         what clinical effects are manifested in fish consumers?




     5.  If high fish consumption is a risk factor for disease, are the




         agent-host-environment interactions defined?  Are these inter-




         actions biologically plausible?  If yes, which link in the




         chain of transmission could be removed for preventive measures?




     Each of the three proposed studies addresses an important aspect of




 the  issue under consideration.  The first study examines the utility of.




 existing data sources for  the identification of mortality and morbidity




 patterns.  The second study will focus on the health characteristics and




 trends  of communities with varied levels of contamination.  The last




 study will discern  the health status of a possibly "high-risk" group.




     There is a paucity of information in the scientific community on




 the  human health effects of chronic low level exposures to environmental




 pollutants.  The three-faceted approach outlined above can help to




 increase our understanding of this vital issue.






                                 138

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                                       143

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                                         145

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                                        146

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                               Addendum








             Survey of Large Cohort of Commercial Fishermen




A.  Introduction




         With the demonstration of the apparently more cost-effective




    telephone interview approach (Protocol I) in the pilot feasibility




    study, it was decided to proceed with a study of a large commercial




    fishermen cohort using an improved questionnaire (Appendix II) based on




    experiences with the pilot questionnaires.  This was possible with the




    time remaining in the basic contract.




         There follows a detailed description of this survey and its




    preliminary results.  It should be noted that the completion of this




    initiated study, in accordance with  the analytic proposals in




    Appendices IV and V, is contingent upon renewal funding.  In addition,




    the proposed coding instructions in Appendix IV apply to the questionnaire




    used in the survey of the large cohort of commercial fishermen, whereas




    the analytic proposals in Appendix V apply to the questionnaires used




    in the small-scale pilot study.




         To evaluate the potential size  of a cohort of commercial fishermen




    it was decided to compile a list based upon all available records in




    the Great Lakes states.  This procedure would hopefully provide




    additional information regarding cohort size.
                                    147

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B.  General Description of the Cohort

         During January and February of 1982 the study supervisor traveled

    to the states of Indiana, Ohio, Pennsylvania, and Wisconsin to obtain

    listings of potential commercial fishermen.  Listings from Illinois,

    Michigan, Minnesota and New York could be mailed to the Division and thus

    visits were not required.

         DNR and license bureau records varied in their completeness, but

    all eight states were able to provide listings back to 1975.  In many

    states, roster entries included company names such as Steve Phalen

    Fisheries and H and G Fish Company.  These entries pose  a problem as

    any number of fishermen and crew members can be employed under this

    blanket company license and will not appear as individual license holders.

         Table 29 describes the availability of records and approximate number

    of commercial fishermen by state.


         Table 29.  Historical Perspective of Commercial Fishing Records
State
Ohio
Illinois*
Indiana
Minnesota
Michigan
Wisconsin
Pennsylvania
New York

Availability Approximate Number
of of
Records Commercial Fishermen
back to 1968
back to 1975
back to 1974
back to 1943
back to 1971
back to 1971
back to 1961
back to 1971
Dunkirk Station
back to 1971
Cane Vincent Station
Total
200
5
80
250
300
300
200
150
86
1,571
+ 75* (Illini
                                                            1,646
          *Have  requested  remaining  records back  to 1970; DNR official stated
          that he would guess approx.  75 commercial fishermen held licenses
          from 1970-1975.

                                  148

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         The results of this investigation indicate that there are




    approximately 1,600 independent commercial fishermen from the eight




    Great Lakes States dating back as far as 1968.  However, for initiation




    of this survey the lists detailed on page 91 of Report II were used




    to generate study participants.  It can be assumed, therefore, that




    the use of the lists dating back to 1968 would provide another 600



    individuals.




         For purposes of this survey it was decided to restrict study




    eligibility to individuals possessing a commercial fishing license




    from any of the eight Great Lakes States and fishing Great Lakes waters.




    As a result of eligibility requirements, individuals employed under a




    company license, inland waters commercial fishermen and crew members




    of license holders were not included in the survey.  The use of these




    individuals in an expanded study would substantially increase cohort size.




C.  Survey Implementation




         Five  Senior Survey interviewers were hired and began interviewing




    November 13, 1981 after a three day training session conducted by the




    project supervisor.  General principles of interviewing, field pro-




    cedures, and question-by-question specifications were reviewed during




    the first training session, followed by role plays, mock interviews and




    the distribution of assignments during the second session.




         Interviewers were given 10-20 assignments each week and  the study




    supervisor met with each interviewer once a week to distribute new




    assignments and review the previous week's completed interviews.




    Detailed information regarding study materials and procedures can be




    found  in Appendix III.
                                    149

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        Each completed questionnaire was edited by the study supervisor




   for errors, omissions and contradictory and/or confusing data.  When




   this occurred,  the supervisor or interviewer re-contacted the respondent




   to obtain the correct information.  Not only did  this  editing process




   produce as  thorough and complete an  interview as possible,  it  also




   provided the study supervisor with an accurate ongoing evaluation  of




   the interviewer's work.




        Tracing was not a major problem in the Great Lakes Fishermen




   Health Study as the lists of potential commercial fishermen  were obtained




   in 1980.  Respondents who had moved from  the last known address were




   usually located with few major  problems since  the commercial fishermen




   tended to be an informed, close-knit community.   In addition to identi-




   fying respondents who had moved, fellow fishermen were invaluable  in




   supplying information about vacationing respondents and confirming or




   negating  the commercial fishing status of unavailable-respondents.




        Due  to time constraints no attempts  were  made  to validate  the




    respondents' medical history although  consent  forms were  requested from




    each participant.  The  focus of this survey was  to  collect  complete




    information on  as many  fishermen and  their families as possible via the




    Great  Lakes Fishermen Health Survey (see  Appendix II).




D.  Preliminary Survey Results




         From an original roster of 1,025  licenses 71 were eliminated




    because they were  owned by  companies.   Of the  remaining 954  individuals




    with licenses,  241 were ineligible because they  did not fish commercially.




    The reasons why individuals possessed  a commercial  licences  but did




    not fish include:
                                  150

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     1.   The individual had taken out a license, but never used'it




         due to the prohibitive cost of equipment and DNR regulations.




     2.   The individual held the license in name only while another




         family member(s)  did the actual fishing.




     3.   Depending on the  state or particular area, owners of charter




         •boat services, hatcheries, and bait and tackle shops were




         either required to purchase a license or held a license




         "just in case".




     4.   The individual fished on inland rivers and lakes only.




     Thirty one individuals were deceased and another 41 were unavailable




during the time of interviewing due to winter vacations and/or requested




disconnected telephones.  Repeated attempts to contact  the unavailable




individuals were made throughout the duration of the interviewing phase.




     Fifty one individuals initially refused to participate in the



study.  Of these 51, 10 were converted into complete interviews




achieving a refusal conversion rate of 19.6%, leaving a total of 41




refusals.  Six individuals could not be located through tracing efforts.




     In summary, there was an original roster of 1,025 licenses which




provided 675 eligible study participants.  Of the  675, interviews were




obtained on 587 individuals for a  response rate of 87%.  Out of 587




respondents, 483 fishermen had wives (wives who were also fisherwomen




are included in this category), 75 had no wives  (i.e., wives were




deceased, divorced over 10 years or single, never  married) and 29  (6%)




of the respondents wives refused to participate in the study.  The




response rate among wives of study participants was 94%.
                               151

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     Table 30 presents the general results of the survey and Table 31
presents the distribution of eligibles, respondents and response rates
by state.
     Table 30.  Breakdown of Study Eligibles, Respondents and
                    Survey Response Rates
     Original Sample
        Company license
        Ineligible
        Deceased
        Other*
1,025
   71
  241
   31
    7
  675   Study Eligibles
        Refusals
        Unavailables
        Lost to follow-up**
   41
   41
    6
                                 587
        Study Respondents
        Response Rate = 87%
        Fishermen w/o wives
        Refusals (wives)
   75
   29
                                 483
        Study Respondents (wives)
        Response Rate = 94%
        *0ther  represents duplicate names
       **Lost individuals could not be located through tracing efforts
                               152

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       Table 31.  Distribution of Study Eligibles, Respondents
                    and Response Rates by State*

Original Sample
Ineligibles
Deceased
Other
POSSIBLE
INTERVIEWS
Refusals
Unavailables
Lost
COMPLETED
INTERVIEWS
IL
5
0
0
0
5
1
2
0
2
IN
63
19
1
0
43
3
3
1
36
MI
147
39
1
3
104
5
11
1
87
MN
101
16
6
0
79
6
2
1
70
NY
49
18
0
0
31
1
4
0
26
OH
no
45
0
2
63
4
6
1
52
PA
41
18
0
0
23
1
1
0
21
WI
438
86
23
2
327
20
12
2
293
Response Rate     40%


                 0.3%
                          84%
         84%     89%     84%
83%     91%     90%
% Contribution
to total cohort
6.1%   14.8%   11.9%    4.4%    8.9%    3.6%   49.9%
*Fishermen only, wives not included in this distribution
                                   153

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     the responses of survey participants regarding fish consumption




practices, number of years fish have been consumed with this frequency




and preferred fish species is presented in Table 32.  In general, the




responses confirm the results of the pilot study in that commercial




fishermen and their families consume large quantities of fish and hava




been doing so for many years.  In addition, the preferred fish speciea




are those which have demonstrated elevated levels of PCE contamination




(see Report Z).




     These findings support those from the pilot study and suggest




that a population of commercial fishermen may provide valuable in-




formation regarding potential health risks associated with ingestion




of organic contaminants via fish.




     It is recommended that the study be expanded to include individuals




employed by commercial fishing companies and crew members of commercial




fishermen as eligible study participants.  Finally, the data collected




should be thoroughly analyzed in accordance with proposals in Appendices




IV and V  to evaluate potential associations between fish consumption




and health among fishermen and their families.
                                154

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Table 32.  Preliminary Results for Great Lakes Study
Distribution of study
the number of fioh
Number of fish
meals consumed
Hever
1-2 times/week
3-5 times/week
6-7 times/week
<1 time/month
1 time/month
2-3 tinea/month




respondents regarding
meals consumed
Number of Study
Respondents %
9 1.5
340 57.9
76 12.9
12 2.0
29 4.9
40 6.8
81 13.8




Distribution of study respondents
regarding the number of years fish
were consumed with this frequency
Number of years
fish consumed with
this frequency
0-5
6-10
11 - 15
16 - 20
21 - 30
31+
31+




Number of study
respondents %
44 7.5
76 12.9
39 6.6
39 6.6
90 15.3
299 50.9





Types of fish most often mentioned
as consumed by study respondents
*Number of study
Fish species respondents
Perch 344
Lake Whitefiah 204
Lake Trout 143
Lake Herring 71 ^
Smelt 59 3
Chub 57
Walleye 45
Catfish 33
Salmon 24
Burbot 22
*Number* do not total 587
because individuals mentioned
more than one species.

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                 APPENDIX I
Rates and Ratios for Thirty-five Cancer Sites
      for "Urban and Rural" and "Rural"
      Analyses in the Great Lakes Basin

-------
                                               CANCER SITE:  LiP
    Urban and Rural Counties
|~T  Rural Counties  (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.
White White Non-white Non-white
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
»TM-k" ti f. Averaged 1st order rate.
RISK raulO Ot ~ : T : , I
Averaged 2nd order rate
"rta" i- i i - -f AveraSed 1st order rate.
KISK ratio or . _ •- :
Averaged 3rd order rate
„„,.,„ . , j. Averaged 2nd order rate
Averaged 3rd order rate
Sum of weighted rates:
"W-lnhtsd risk" ratio of Avera8ed lst order rate-
weignueu risK xauxu o± T^ ^T : , .. .
& Sum of weighted rates
"uMoi -r---i ^-rcir" rar-i- nf Ave^aged 2nd order rate.
weisnted risK. ratio or _ , 	 ?—. 	 . :
5 Sum of weighted rates
"U-lal -tsd ri-k" rati- of Averased 3rd order rate
Weighted risk ratio of s^ Q£ weighted rates .
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

Males Females Hales Females
0.3491
0.3910
0.3744
0.8928
0.9324
1.0443
0.3696
0.9445
1.0579
1.0130





0.0110
0.0141
0.0287
0.7801
a. 0124
0.0000
0.2742


0.3833 0.0452.
0.4878


0.0227 0.1573
0.4846
0.6211
1.2643



X

0.0788


1.7437





0.0064
0.0050
0.0012
1,2800
5.3333
4.1667
0.0034
1.8823
1.4706
0.3529






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                                                CANCER SITE:  Salivary Glands
C2I  Urban and Rural Counties

|~"7  Rural Counties  (counties having urban
    centers with 100,000 inhabitants are
    excluded  from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"tl-k" ratio of Avera*ed 1st order rate
ZW.SK LCLUJ.O OI7 -"- . - r^^ 7 •
Averaged 2nd order rate

"rijk" rar-r- f Avera*ed lst order rate-
KJ.SK ratio 01 , _ , . :
Averaged 3rd order rate
"riik" rnri- -' Avera8ed 2nd order rate
Risk ratio o. Averaged 3rd Qrder rate.

Sum of weighted rates:
"l^iEhtnd risk" ratio of Avera§ed lsc order rate-
wexgnt-cu Z.LSK rauio OL T ^ ~ .
Sum of weighted rates
"u til r-J I-' k" --Li- -f AveraS£d 2nd order race
wexgciueu rxSK rauu.o or ^ ,. ; ;
6 Sum of weighted rates
"U -Ijl r^af r-f^t" vjri- of AveraSed 3rd order rat£.
we-Lgtiteu risK. ratio 01 „ **, :
0 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
0.4229
0.3397
0.3991
1.2A49

1.0596
0.8512

0.3991
1.0596
0.8512
1.0000



i
t
0.2318
0.1971
0.2210
1.1755
0.0967
0.0186
0.2127
5.1989
I
1.0484 0.4546
0.8918 0.0874

0.2214 0.1574
1.0470
0.8902
0.9982




0.6143
0.1182
1.3513




0.0830
0.6525
0.3129
0.1272

0.2653
2.0860

0.2380
0.3487
2.6155
1.3147





-------
                                           CANCER SITE:  Nasopharynx
-Urban and Rural Counties

Rural Counties  (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
White White Non-white Non-white
i
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ri-L." ii- of Avera*ed lst order rate-
IV.LSK ratio OJL ~ _- 0 . . • i
Averaged 2nd order rate !
,,_. ,,. . _f Averaged 1st order rate.
Risk ratio of Avgraged 3rd Qrder tate-
„-..,,, , f Averaged 2nd order rate.
Risk ratio of Averaged 3rd Qrder rat£.
Sum of weighted rates:
...... . ._,„ . , Averaged 1st order rate.
Weighted risk ratio of Sum £f weighted rate£ .
..„ . . . . , „ . , Averaged 2nd order rate.
Weignted rxsk ratio of Sum Qf weighted rates •
.... . . .,",, r Averaged 3rd order rate
Weighted risk ratio of Sum Qf W£ighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
Males Females Males Females
0.2744
0.3223
0.2758
0.8514
0.9953
1.1690
0.2802
0.9793
1.1502
0.9843





0.1072
0.0952
0.0829
1.1260
0.2337
0.0639
0.0814
3.6573
1.2931 : 2.8710
i
1.1484 ; 0.7850
i
0.0904 ' 0.1408
1.1858
1.0531
0.9170

X



1.6598
0.453-8
0.5871





0.0387
0.0050
0.0280
7.7400
1.3821
0.1786
0.0312
1.2404
0.1603
0.8974






-------
CANCER SITE:  Tongue and
C2J Urban and Rural Counties
Q Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
nr -f_,k.. r^r±Q nf Aver*8ed lst order rate.
K_Lstc ratio or - n ~ 	 . •
Averaged 2nd order rate
"Risk" ratio of Averaged 1st order rate;
Averaged 3rd order rate
„->,,, ... - Averaged 2nd order rate.
Risk ratio or Averaged 3rd Qrder rate-
Sum of weighted rates:
"u ii.1 r J riLA" ratio of AveraS£d 1st order rate.
weignteu risK. ratio or " .
0 Sum of weighted rates
"u i-i i J l^" rar-fo of Avera2fid 2nd order rate
Weighted j isk ratio of gum of weighted rates •
"u ill I r-f-L" rsri- of Avera8ed 3rd order rate.
Weighted risk ratio of Sum Q. WQighte(j rates •
Trends
The averaged first order rate is greater
Chan the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.


White
Males
3.8479
3.3003
3.0066
1 1660
•I, * J»W V *•/
1.2798
1.0976

3.2539

1.1825

1.0143

0.9240

X
|


White
Females
0.9223
0.8795
0.7318
1 0487
X * V*TW *
1.2603
1.2018

0.7954

1.1595

1.1057

0.9200

X



Non-white
Males
1.1997
1.8376
4.4046
0 6529
\J * \J J imj
0.2724
0.4172

3.0113

0.3984

0.6102

1.4627





Non-white
Females
0.4874
0.5698
1.6990
0 8554
\J * Uj^^r
0.2869
0.3354

1.1753

0.4147

0.4848

1.4456





-------
                                                CANCER SITE:  Es°PhaSus
[33  Urban  and  Rural  Counties

j~"T  Rural  Counties  (counties having urban
    centers  with 100,000 inhabitants are
    excluded from analysis.)
'
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
n»,.f_iii tJ ,e Averaged 1st order rate.
Averaged 2nd order rate
„-,-,,. , _ .f Averaged 1st order rate.
K1SK. ratlO 01 T 	 . _ ., 	
Averaged 3rd order rate
,,-, . ,it _p Averaged 2nd order rate.
Averaged 3rd order rate'
Sum of weighted rates:
it,. _.f-, , • in _ ^j- r Averaged 1st order rate.
weigriteu risfc ratio ot ^ .. . r , '•
Sum of weighted rates
,,UA, ., , ._,.,i . . _f Averaged 2nd order rate.
Weighted risk ratio of SUQ Q£ weight£;d rates •
"•J Jii i i i • L" i- i i f Averased 3rd order rate
Heiaut?u risic ratio or zr^ ^^ ^ , , .
* Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.


White
Males
4.2989
3.2781
2.9805
1.3114

1.4423


1.0998
3.3514

1.2827

0.9781
00 ftf\ i
.8893

K



White
Females
0.9902
0.7857
0.7503
1.2603

1.3199


1.0473
0.8152

1.2147

0.9638
Or\f\f\i
.9204

X



Non-white
Males
8.2348
5.0238
5.8199
1 6392
-*• • \J^J y 6m
1 4149
-± • *T J."+ J

0.8632
6.7378

1.2222

0.7456

0.8626





Non-white
Females
1.0127
25.7817
0.6422
O^QOfl
• J?4o
1 5769
J> * — * * U 7

40.1459
1.8978

0.5336

13.5850

0.3384






-------
                                               CANCER SITE:  Stomach
DQ  Urban  and Uural  Counties

D  Rural  Counties  (counties having urban
    centers with  100,000  inhabitants are
    excluded from analysis»)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ili-k" ratio of Avera8£d 1st order rate.
Averaged 2nd order rate
HTJ-I i-ii ^. - Averaged 1st order rate.
Averaged 3rd order rate'
»« i n ^j f Averaged 2nd order rate
Averaged 3rd order rate' :
Sum of weighted rates:
Mr, -L - j _, i n ^j c Averaged 1st order rate
Sum of weighted rates
•|T, . . , . , „ . c Averaged 2nd order rate
weignteu nstc rauio or r ^s 7 rr j . .
6 Sum or weighted rates
"u-r-l i l i-i^" i-arl- -f Avera^ed 3rd order rate.
Weighted risk ratio of Sum Q{ we±ghted rates .
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.


White White Non-white Non-white
Hales Females Males Females
19.3606
16.6168
7.1509
1.1651
1.3525
1.1609
15.8535
1.2212
1.0481
0.5772

X

9.2993
7.6511
4.8292
1.2154
23.9864
19.9937
7.4590
1.1997
1.2395 1.7868
1.0198 1.4894
7.9769 17.8695
1.1658
0.9592
0.6054

X
.
i
i
1.3423
1.1189
0.4174

X



8.8318
14.5268
14.5268
0.6080
1.3334
2.1933
7.8361
1.1271
1.8538
0.4773






-------
                                               CANCER SITE:  Large Intestine
33  Urban and Rural  Counties

O  Rural Counties  (counties having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
1
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
„„_, _, „ 	 ^ _ _c Averaged 1st order rate.
Averaged 2nd order rate
„ ,„ . . - Averaged 1st order rate.
Averaged 3rd order rate*
„... ,,, rl. - Averaged 2nd order rate.
KISK ratio ot , , _ , . :
Averaged 3rd order rate
Sum of weighted rates:
H, , j , ^ j ., -i ii _-_•- -e Averaged 1st order rate.
Weisnt&u risk ratio oz ^ ' » . , , :
0 Sum or weignted rates
"TJ • i , j rl-L" rnri- -f Avera2Qd 2nd order rate.
weisnteu nsK racxo or _ - "; . , .
6 Sum of weighted rates
it,, . , j • , ii ^j c Averaged 3rd order rate
weighted nsic ratxo OE r 	 ~i 	 . . , :
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than che second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females
16.5203
16.0048
15.8063
1.0322
1.0452
1.0125
16.0110
1.0318
0.9996
0.9872
X


16.5682
16.5117
16.9205
1.0034
9.7122
11.2259
11.0775
0.8652
0.9792 0.8767
0.9758 1.0134
16.7893 10.5440
0.9868
0.9835
1.0078



0.9211
1.0647
1.0506



15.9118
9.1557
15.3798
1.7379
1.0346
0.5953
15.3129
1.0391
0.5979
1.0044




-------
                                                CANCER SITE:
                                                              Rectum
E2J  Urban a-nd Rural Counties

Q  Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged  1st order rate:
Averaged  2nd order rate:
Averaged  3rd order rate:
 "Risk"  ratio of f^aged 1st order rate.
                 Averaged 2nd order rate
 "•04 -I."
 Risk"  ratio of
Averaged 1st order rate.
Averaged 3rd order rate'
 „_,  , „     .    , Averaged 2nd order rate
 Risk  ratio of	&i ••; ,  .	•:	T~:
                 Averaged 3rd order rate
Sum  of weighted rates-:
 "Weighted  risk"  ratio of Averaged 1st order rate;
                           Sum of weighted rates


 "Weighted  risk"  ratio of Averaged 2nd order rate
     °                      Sum of weighted rates


 "Weighted  risk"  ratio of Averaged 3rd order rate;
                           Sum of weighted rates
Trends

The  averaged  first  order rate is greater
than the  second  order rate which in turn
is greater  than  the third order rate.

The  averaged  third  order rate is greater
than the  second  order rate which in turn
is greater  than  the first order rate.
                                                      White       White     Non-white   Non-white
                                                      Males      Females      Kales      Females
                                     7.7925
                                     7.8417
                                     7.2972
                                     0.9937
1.0679
                                     1.0746
                                     7.4809
                                     1.0416
            4.8053
            5.0198
            4.7094
            0.9573
            4.7652
            1.0084
                                     1.0482   |   1.0534
                                     0.9754
            0.9883
            2.2489
            9.1949
            5.0750
            0.2446
1.0204   !   0.4431
            1.0659      1.8117
                                                                              4.1549
            0.5413
                        2.2130
            1.2214
3.1272
1.1791
3.1700
1.8191
0.9865
                        0.5423
                        3.0892
1.0123
                        0.3817
1.0262

-------
                                               CANCER  SITE:
                                                             Liver
rT[  Urban and Rural  Counties

CJ  Rural Counties  (counties  having  urban
    centers with.  100,000  inhabitants  are
    excluded from  analysis.)
Averaged  1st  order  rate:
Averaged  2nd  order  rate:
Averaged  3rd  order rate:
 "Risk"  ratio of  Averted  1st  order  rate;
                 Averaged  2nd  order  rate
 "   -"
              of
 "     "
  Risk"  ratio of
Averaged 1st order rate.
Averaged 3rd order rate'
Averaged 2nd order rate.
Averaged 3rd order rate'
 Sum of  weighted rates:
 "Weiahted risk"  ratio of Avera8ed 1st order  rate,
  weighted risk  ratio or  Sum Q£  weighted  rates
 "Weighted .risk"
      of
 "Weighted risk"  ratio of
Averaged 2nd order rate
 Sum of weighted rates


Averaged 3rd order rate.
 Sum of weighted rates
 Trends

 The averaged first order rate is greater
 than the second order rate which in turn
 is  greater  than the third order rate.

 The averaged third order rate is greater
 than the second order rate which in turn
 is  greater  than the first order rate.
White
Males
5.1953
4.6395
4.8028
1.1198
1.0817
0.9660
4.8874
1.0630
0.9493
0.9827



White
Females
6.5479
6.0074
6.2763
1.0900
1.0433
0.9672
6.3187
1.0363
0.9335
0.9933



Non-whi te
Males
6.6440
5.8985
5.6348
1.1264
1.1791
1.0468
6.0473
1.0987
0.9754
0.9318



Non-white
Females
5.9107
5.0079
4.8715
1.1803
1.2133
1.0280
5.2839
1.1186
0.9478
0.9220




-------
CANCER SITE:
             Pancreas
D8 Urban and Sural Counties
Q Rural Counties (counties having urban
Centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ttl-k" rati- tf AveraSed la* order rate.
AxSix zawZO Ox . . ^ - , •
Averaged 2nd order rate
"ri£k" nMti- f Averaged 1st order rate.
Averaged 3rd order rate
•Ti-L" L tf £ Averaged 2nd order rate.
Risk ratio of Avaraged 3rd order rate-
Sum of weighted rates:
"u-ltthr-l --1-k" raiij -f Avera8ed lst order rate
weignueu riLSK racxo 01 _ - ; , , t
° Sum of weighted rates
"U \i\ L^J i-I V" raH of Avera2fid 2r>d order rate-
weignteu rxstc ratio on ^ - ^ t T ^i
6 Sum of weighted rates
"U --tahr 1 ri -1" rari^ cf AveraSed 3rd order rate
weiKuuPa risK ratio or „ "_ , , :
0 Sum of weightea rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females
9.2717
8.9293
8.4645
1.0383
1.0954
1.0549
8.7206
1.0632
1.0239
0.9706

X

6.0605
5.5112
5.4478
1.0997
3.8775
5.7570
7.7707
0.6735
1.1125 0.4990
1.0116 0.7409
6.8162 5.6109
0.8891
0.8085
0.7992

X

t
0.6911
1.0260
1.3849




10.7675
2.9088
3.9151
3.7017
2,7502
0.7430
8.8068
1.1613
0.3303
0.4445





-------
CANCER SITE:
             Nose and Middle Ear
23 Urban and Rural Counties
| i Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ni-k" ratio of Avera§ed lst order rate-
Averaged 2nd order rate
"Risk" ratio of Averaged 1st order rate
Averaged 3rd order rate
„„.-,.„ . ±. -f Averaged 2nd order rate
EtiSr<. ratio or . .. « . , .
Averaged 3rd order rate
Sum of weighted rates:
.IT- in, , , Ll-,« rnLi -f Avera8ed 1st order rate.
6 Sum of weighted rates
"u ijli 1 tl" rir-t- -f AveraSed 2nd order rate.
weigtiteu risK ratio 01 r ™-r 	 ; 	 ,
6 Sum of weighted rates
„,, . . . , „ . ,. Averaged 3rd order rate
weignteu nsic ratio or _ , , . •; .
Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
0.3829
0.5282
0.4440
0.7249
0.8624
1.1896
0.4369
0.8764
1.2090
1.0162



0.2280
0.1785
0.2280
0.3576
0.4094
0.2673
r
S
1.2273 0.8735
1.0000 : 1.3378
0.7829 1.5316
0.2051
0.1116
0.8703
1.1116



0.3100
•
1.1535
1.3206
0.8622



9.4382
0.2148
0.1796
2.0400
2.4399
1.1960
0.2823
1.5522
0.7609
0.6362




-------
                                                CANCER SITE: Larynx
Q£j Urban and Rural Counties

l~"t Rural Counties (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged  1st order rate:
Averaged  2nd  order rate:
Averaged  3rd  order rate:
 "Risk"  ratio of Averaged 1st order rate.
                 Averaged 2nd order rate
 Risk"  ratio  of
Averaged 1st order rate.
Averaged 3rd order rate'
 „,,.,„  _-_.,„  . Averaged 2nd order rate.
 Risk  ratio of Avera|ed 3rd order rate=
 Sum  of weighted rates:
 ,„. .  ,   ,   .  . „   _.    - Averaged 1st order rate
 Weighted  risk  ratio of —	°~^	r—r—3	:
    *                     Sum of weighted rates


 II,. .  ,   ,   .  , ii    j    r Averaged 2nd order rate
 Weighted  risk  ratio of •—	^	7-r—3	:
    6                     Sum of weighted rates


 «„ .  ,   j   *  , it   _.,    £ Averaged 3rd order rate
 "Weighted  risk" ratio of  Sum gf weighted rates :
 Trends

 The  averaged first order rate is greater
 than the  second order rate which in turn
 is greater  than the third order rate.

 The  averaged third order rate is greater
 than the  second order rate which in turn
 is greater  than the first order rate.
                                                      White       White     Non-white   Non-white
                                                      Males      Females      Hales      Females
                                                      2.3800
                                                      1.8688
                                                      1.7748
                                     1.2736
                                                      1.3401
                                  !   1.0530
                                                      1.9407
                                                 0.1546
                                                 0.2131
                                                 0.1774
0.9516
0.8715
1.2012
                                                 0.1752
                                     1.2264   j   0.8824
                                     0.9629   !   1.2163
                                     0.9145
1.0126
1.8640


1.9599


1.6403


0.9516



1.1370


1.1948



1.7447


1.0689


1.1233


0.9402
0.2067


0.1952


0.9280



1.0589



0.2227



0.2103



0.6136


0.3369


0.3181



1.5124

-------
                                               CANCER
SITE' Trachea, Bronchus and Lung
[33  Urban and Rural Counties

d[  Rural Counties  (counties having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
White White Non-white Non-white
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"r-r^k" r^m- of Avera8ed lst order rate
Averaged 2nd order rate
"cr i" -i i - -f Avera§ed lst order rate-
Risk ratio of Averaged 3rd order rate-
"ri-k" AI i f Avera^ed 2nd order ra*e
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:

"U f-1 I 1 -isk" r-Ll-ia jf Avera!?ed lst order rate -
weigntect nstc ratio or ~_
e Sum of weighted rates
"U f 1 . 1 -Uk" rari- rf Avera8ed 2nd order rate-
Weighted risk ratio or Sum Q£ we±ghted rates -
'"J i ii J l-k" -iti- -f Averased 3rd order rate.
e Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

Males Females Males Females
32.9002
30.6619
28.5293
1.0730
1.1532
1.0747
29.8764

1.1012
1.0263
0.9549

X



i
5.1322
5.1388
5.1546
0.9987
29.6472
21.9218
27.7351
1.3524
0.9956 1.0689
0.9969 0.7904
5.1473 28.2114
j
0.9971
0.9983
1.0014



X


1.0509
0.7771
0.9831






5.7151
3.2147
5.0540
1.7778
1.1308
0.6361
5.2313

1.0925
0.6145
0.9661







-------
                                               CANCER SITE: Breast
ESI  Urban  and Rural  Counties

Q  Rural  Counties  (counties  having urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
»~, rV, t, f Averaged 1st order rate.
IU.SK ratio oz , — „ . , :
Averaged 2nd order rate
"rj-k" I--I.T f Avera*ed lst order rate
Averaged 3rd order rate
,,r, ,,, r .. f Averaged 2nd order rate.
Risk ratio of Avfiraged 3rd order rafce.
Sum of weighted rates:
"u -TCI -t^J t-lak" rjti- af Avera8ed 1st order rate.
weiCnueu rxsK racjuo or _ - . , . .
ft Sum of weighted rates
"u iui i^l il L11 LI -f Avera2£d 2nd order rate-
& Sum of weighted rates
"U ici t-j rick" i-iti- n- Averaged 3rd order rate
Weighted risk ratio o* Sum flf weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater Chan the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
0.2661
0.1841
0.2429
1.4554
1.0955
0.7579
0.2428
1.0960
0.7582
1.0004


25.5191
25.0840
24.1887
1.0173
0.2154
1.7380
0.2499
0.1239
j
1.0550 0.8616
1.0400 6.9520
24.6189 0.3082
1.0366
1.0189
0.9825
X

t
0.6989
5.6392
0.8108



23.0836
27.6612
17.5420
0.8345
1.3159
1.5769
20.1563
1.1452
1.3723
0.8703




-------
                                               CANCER SITE:  Cervix Uter±
CXI  Urban and Rural  Counties

£2  Rural Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)

Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"list" ratio of Avera§ed lst order rate-
AlSK ratZLw Oi : r : _ «
Averaged 2nd order rate
»-. ,« -., 	 f Averaged 1st order rate.
Rxsk ratio of Averaged 3rd Qrder rat£-
„-, ,„ - ,-, - -f Averaged 2nd order rate.
Risk ratio of Averaged 3rd Qrder rate-
Sum of weighted rates:
„., , , , , , ii . /- Averaged 1st order rate
5 Sum of weighted rates
ltr, , . , . , ii . ,• Averaged 2nd order rate
Weignted risk ratio of Sum Q£ weightei rates .
.,._... , . ., „ . . . _f Averaged 3rd order rate
Weighted risk ratio of Sum Qf weighted races -
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White
Hales


















White
Females
8.1263
7.8110
7.9403
1.0404

1.0235

0.9837

7.9749
1.0190

0.9794

0.9957



Non-white
Males


















Non-white
Females
15.3534
23.0495
9.4707
0.6661

1.6211

2.4338

12.3712
1.2411

1.8631

0.7655




-------
CANCER SITE:
              Corpus Uteri
CSJ Urban and Rural Counties
|~~? Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
i.r.i.Lii 	 , 	 e Averaged 1st order rate.
Averaged 2nd order rate
"Itifik" ratio of AveraS?ed 1st order rate.
Averaged 3rd order rate
"Risk" ratio of Averaged 2nd order rate; |
Averaged 3rd order rate
Sum of weighted rates:
"XJpichtPd risk" ratio of Avera8ed lst order rate-
Sum of weighted rates
"Up-fahtpJ ri£k" ritio of Averased 2nd order rate
6 Sum of weighted rates
"IkiahttJ riEt" ratio of Avera^ed 3rd order rate
WaigLttJ iisfc latio of gum o£ weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Hales Females Males Females
i
6.5222
6.8524
6.8903
0.9519
0.9466
0.9945
6.7925
0.9603
1.0088
1.0144
X

11.5115
10.6140
9.0597
1.0846
1.2706
1.1716
10.0872
1.1412
1.0522
0.8981

-------
                                               CANCER SITE:  Ovary and Fallopian tube
    Urban and Rural Counties

    Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of Averaged 1st order rate;
                Averaged 2nd order rate

„_.  . „    J    , Averaged 1st order rate
 Risk  ratio of	°  , ,  .	:
                Averaged 3rd order rate


„_.  . „    .    - Averaged 2nd order rate
 RISK  ratio or Avera^ed 3rci order rate =
Sum of weighted rates:
"Weighted risk" ratio of
Averaged 1st order rate f
 Sum of weighted rates
"Weighted risk" ratio of Averaged 2nd order rate;
                          Sum of weighted rates


IIT, .  ,    ,   - , ii     .   r Averaged 3rd order rate
 Weighted risk  ratio of —	°-z	 .    .	:
    0                     Sum of weighted rates
Trends

The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.

The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
                                                     White
                                                     Males
                                        White
                                       Females
           Non-white
            Males
Non-white
 Females
8.3Q35


8.6594


8.4251


0.9589


0.9856



1.0278



8.4175


0.9865


1.0287



1.0009
 4.4672


 5.9559


 6.3573


 0.7501


 0.7027


 0.9368



 5.6004


 0.7977


 1.0635


 1.1351

-------
                                                CANCER SITE:
    Urban and Rural  Counties

    Rural Counties  (counties having  urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order  rate:
Averaged 2nd order  rate:
Averaged 3rd order rate:
"Risk" ratio of Averaged  1st  order  rate;
                Averaged  2nd  order  rate
"Risk" ratio of
"Risk" ratio of
                Averaged  1st  order rate.
                Averaged  3rd  order rate*

                Averaged  2nd  order rate.
                Averaged  3rd  order rate'
Sum of weighted  rates:
 ...  . ,    ,   . ,„    __.    ,  Averaged 1st order rate
 Weighted risk   ratio of  —	«-=	7-7-—-:	:
    °                      Ctvrn s\4-  r.T/a-i *»ri *-AJ-* *•«> 4- A»
                           Sum of  weighted rates
„,,  . ,    _.   . , it     .    c  Averaged  2nd order rate
 Weighted risk   ratio  of  —	°-:	-r—r—7	:
    e                      Sum of weighted rates
"Weighted risk"  ratio  of  Averaged  3rd order rate;
    6                      Sum of weighted rates
Trends

The averaged  first  order rate is  greater
than the second  order  rate which  in turn
is greater  than  the third order rate.

The averaged  third  order rate is  greater
than the second  order  rate which  in turn
is greater  than  the first order rate.
                                                      White       White     Non-white   Non-white
                                                      Males      Females      Males      Females
18.2989


18.5688


18.3151


 0.9855



 0.9991



 1.0138



18.3369


 0.9979



 1.0126   I



 0.9988
17.2375


15.9260


21.8424


 1.0823



 0.7892



 0.7291



19.7328


 0.8735



 0.8071



 1.1069

-------
                                                CANCER SITE:
                                                             Testis
33  Urban  and  Rural Counties

V~  Rural  Counties (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
 Averaged 3rd order rate:
 "Risk"  ratio of Averaged 1st order rate
 "Risk" ratio of
Averaged 2nd order rate'

Averaged 1st order rate.
Averaged 3rd order rate'
 "iMefc'i  ratio of Averaged_. 2nd order rate
  Risk  ratio of Averaged 3rd Qrder
 Sum of  weighted rates:
 „„ .  .   .   .  , „    .    , Averaged 1st order rate
 'Weighted  risk" ratio of  '«.•"-	;-.-  .	r	:
     0                     Sum of weighted  rates
 it,, .  .   ,   _,  , 11    .    c Averaged 2nd order rate
 'Weignted  .risk" ratio of  Sum gf weighted rates •


 "Weighted  risk" ratio of Averaged 3rd order rate;
     0                     Sum of weighted rates
 Trends

 The averaged first order rate is greater
 than che second order rate which in turn
 is greater than the third order rate.

 The averaged third order rate is greater
 than the second order rate which in turn
 is greater than the .first order rate.
                                                      White
                                                      Males
0.9401


0.8991


0.9189


1.0456



1.0231



0.9784



0.9223


1.0193


0.9748



0.9963
                                                 White
                                                Females
                       Non-white
                         Males
              Non-white
               Females
   0.0831


   0.0567


   0.1875


j   1.4656
I

!   0.4432
t


•   0.3024
i
I
I
:   0.1399


   0.5940



   0.4053



   1.3402

-------
                                                CANCER SITE:  Kidney
C2I  Urban and Rural Counties

C~T  Rural Counties (counties  having urban
     centers with  100,000 inhabitants are
     excluded from analysis.)
Averaged 1st order rate:
 Averaged 2nd order rate:
 Averaged 3rd order rate:
 "Risk"  ratio of Averaged 1st order rate.
                 Averaged 2nd order rate
  Risk"  ratio of
  Risk"  ratio of
Averaged 1st order rate.
Averaged 3rd order rate'

Averaged 2nd order rate.
Averaged 3rd order race'
 Sum of weighted rates:
 "Weighted risk" ratio of
         Averaged 1st order rate
          Sum of weighted rates
 „,, .  ,   ,   ,  , „    .    , Averaged 2nd order rate
 "Weighted  risk" ratio of  Sum £f weighte,l rates
 „,, .  ,   ,   .  , „    .    - Averaged 3rd order rate
  Weighted  risk  ratio of —	°-^	.  , • •• j,	
     6                     Sum of weighted rates
 Trends

 The averaged first order rate is greater
 than the second order rate which in turn
 is greater than the third order rate.

 The averaged third order rate is greater
 than the second order rate which in turn
 is greater than the first order rate.
White
Males
4.1238
3.6236
3.7703
1.1380
1.0938
0.9611
3.8465
1
1.0721
0.9420
0.9802

i
r
i
White
Females
2.1792
2.0213
2.0801
1.0781
1.0477
0.9718
2.0995
1.0380
0.9627
0.9903
:
|

Non-white
Hales
2.0134
2.3809
2.2870
0.8456
0.8804
1.0411
2.1832
0.9222
1.0905
1.0475


Non- white
Females
0.5323
0.8328
1.7303
0.6392
0.3076
0.4813
1.2221
0.4356
0.6814
1.4158



-------
                                               CANCER SITE:  Bladder
    Urban and Rural Counties

    Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of Averaged 1st order rate.
                Averaged 2nd order rate


"Risk" ratio of Averaged 1st order rate;
                Averaged 3rd order rate
"Risk" ratio of Averaged 2nd order rate;
                Averaged 3rd oraer rate
Sum of weighted rates:
"Weighted risk" ratio of Averaged 1st order rate;
    0                     Sum of weighted rates


n.r ., v  j  j i n    _,   c Averaged 2nd order rate
 Weighted risk  ratio of —r	°-=	j—r—j	:
    *                     Sum of weighted rates
Trends

The averaged first order rate is greater
than the second order rate which in  turn
is greater than the  third order rate.

The averaged third order rate is greater
than the second order rate which in  turn
is greater than the  first order rate.
                                                     White       White     Non-white   Non-white
                                                     Males      Females      Males      Females
6.8957


7.3321


5.8743


0.9405


1.1739


1.2482



6.2875


1.0967
2.5049


2.2967


2.1449
1.0708
2.2523
1.1122
1.1661   j   1.0198
                                                     0.9343
            0.9524
4.8102
5.2621
2.6309
1.0906      0.9141
1.1678   •   1.82S3
2.0001
3.6211
1.3284
            1.4532
            0.7265
1.8569


2.0817


2.6733


0.8920


0.6946


0.7787



2.3279


0.7977


0.8942


1.1484

-------
                                               CANCER SITE:  Skln
    Urban and Rural Counties

    Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of Averted 1st order rate.
                Averaged 2nd order rate
                                                     White       White     Non-white   Non-white
                                                     Males      Females      Males      Females
 Risk" ratio of
"t>4 -I."
 Risk" ratio of
Averaged 1st order rate.
Averaged 3rd order rate'
Averaged 2nd order rate.
Averaged 3rd order rate'
Sum of weighted rates:
"Weighted risk" ratio of Averaged 1st order rate;
    6                     Sum of weighted  rates
urr j ,    _,  j , ti     .,    - Averaged 2nd order rate
"Weighted risk" ratio or -    *£ veighted rates  !
„,,  , ,    ,   . , „     .    , Averaged  3rd order rate
"Weighted risk" ratio  of   Sum *£ weighted  rates  :
Trends

The averaged first order rate is greater
than the second order rate which in  turn
is greater  than the  third order rate.

The averaged third order rate is greater
-than the second order rate which in  turn
is greater  than the  first order rate.
0.9569


1.0196


1.2218


0.9385



0.7832



0.8345



1.1327


0.8448
                                                 0.7774
                                                 0.9110
                                                 0.9804
                                                 0.8533
0.7929
                                                                 0.9292
                                                 0.8436
                                     0.9001   j   0.9886
                                     1.0787
            1.0639
            0.0464
            0.0823
            0.0702
            0.5638
0.6610
            1.1724
                                                 0.9215      0.0613
            0.7569
                        1.3426
            1.1452
0.0103


0.3984


0.3027


0.0258


0.0340


1.3161



0.1926


0.0534


2.0685


1.5716

-------
CANCER SITE:
             Other skin
HT] Urban and Rural Counties
1~~ Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
npj v» -t f Averaged 1st order rate_
Averaged 2nd order rate'
"m" ran- -r Averased 1st order rate
Risk ratio of Averaged 3rd Qrder ratg.
"Y- -i" rjftl -r Averaged 2nd order rate
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:
•i*. „ , _ j j t 11 ^ * Averaged 1st order rate.
Wei2hteu risk ratio or _ _ :™~: 	 t
0 Sum of weighted rates
,,„ • ,. , ,-,-,.. -ir,- -* Averaged 2nd order rate.
weientea nsK ratio 01 • , .
0 Sum of weighted rates
"'.J--t3-r-3 ri^-" -ar-I- -f AveraSed 3rd order rate_
-?eignted rxsk ratio of s^ Qf weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Hales Females Males Females
1.2889
1.3164
1.4384
0.9791
0.8961
0.9152
1.3873
0.9291
0.9489
1.0368



0.5505
0.6241
0.6423
0.8821
2.8011
1.0289
0.4887
2.7224
0.8571 5.7317
0.9717 2.1054
0.6170 1.4306
0.8922
1.0115
1.0410


X
1.9580
0.7192
0.3416



0.1210
0.1402
0.4418
0.8630
0.2739
0.3173
0.3030
0 3993
0,4627
1.4581




-------
CAKCER SITE:   Eye
DQ Urban and Rural Counties
O Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Hink" ratio af Averaf?ed 1st order rate-
Averaged 2nd order rate
MTM-I," r,t-t- nf Avera8ed 1st order rate.
XVJ.SK racio 01 . , „ . , . .
Averaged 3rd order rate
"Risk" ratio of AveraBed 2nd order rate.
Averaged 3rd order rate'
Sum of weighted rates:
"Ifclcht-j ri.sk" ratij jf AveraSed 1st order rate.
wexKitLeu rzsK racxo or _. .. , . . .
Sum of weighted rates
"U^ialit^J --i-k" IAII af Avera«ed 2nd order rate
wexgnceu :.j.sic rauxo or r ^ ; . , .
Sum or weighted rates
urT-^-v^ j MJ -i ii -^-.- _£ Averaged 3rd order rate.
Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White
Males
0.1919
0.2052
0.2380
0 93S2
V • .S -J J 4*
0.8063

0.8622

0.2227

0.8617

0.9214
1f\f ft -T
.0687


X

White
Females
0.1871
0.1490
0.1946
1 ?S57
x . t-jj i
0 9615
\J • J \J ^-J
0 7657
\J • i \JJ 1
0.1881

0.9947

0.7921
1.0346




Non-white
Males
0.0089
0.0345
0.0100
A 2?51
\j . j^jjj*
n 8800
\J * (J*J \J\J
•5 4500
•J • ** J W \J
0.0107

0.8318

3.2243
0.9346




Non-white
Females
0.1484
0.0000
0.1363


i nfifis
J. • UUU w


0.1350

1.0993


1.0096




-------
                                                CANCER SITE-  Brain and Nervous System
TTf  Urban  and Rural  Counties

l~j  Rural  Counties  (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"TM-I-" - • f Averaged 1st order rate.
Averaged 2nd order rate'
,,r. , „ . - Averaged 1st order rate.
K.ISK rauio or . ^ « T _
Averaged 3rd order rate
„ „ - Averaged 2nd order rate
Risk ratio or Averaged 3rd order race-

Sum of weighted rates:
"17 ' 1 I 1 L-k" vjM- -f AVera8£d lst °rder ratS-
Weighted risk ratio of gum Qf weighted rates •
,,„ . . . .,",,. -f Averaged 2nd order rate.
Weighted risk ratio of Sum Qf weighted rates •

it,, - , , , M j <• Averaged 3rd order rate
Weighted risk ratio of gum Q£ weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
4.1597
4.3553
3.9854
0.9551
1.0437
1.0928

4.0683
1.0225
1.0705
2.7555
2.6536
2.6692
2.0993
1.1240
1.6092
1.0384 1.S677
1.0323 1.3046
0.9942 0.0771

2.6897 1.7255
1.0245
0.9866
f
0.9796

I
!
0.9924


1.2166
0.6514

0.9326


4.5234
0.5267
1.0448
8.5882
4.3294
0.5041

2.3823
1.8987
0.2211

0.4386



-------
                                               CANCER SITE: Thyroid gland
CXI Urban and Rural  Counties

1• Rural Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded  from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ttiak" ratio o* AveraSed lst order rate-
Averaged 2nd order rate
ti-nj i ii . j f Averaged 1st order rate
K.J.SK ratio 01 — 	 — : — _ , 	 . :
Averaged 3rd order rate
"III ak" r^M- -f Averased 2nd order rate
Averaged 3rd order rate
Sum of weighted rates:
n,, j , n j . Averaged 1st order rate
Weiiznted risR ratio or _ ^ 	 ; — : 	 _ :
Sum of weighted rates
,lr, j ,t ^j r Averaged 2nd order rate
* e Sum of weighted rates
"U^'-Ii ' i-t" Ya.i±- -• Averaaed 3rd order rate.
0 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Hales Females
0.4902
0.5173
0.3631
0.9476
1.3500
1.4247
0.4117
1.1907
1.2565
0.8819




0.7535
0.7189
0.7252
1.0481
0.1848
0.0345
0.3715
5.3565
1.0390 0.4974
0.9913 ;' 0.0929
0.7318 0.2813
1.0296
0.9824
0.9910




0.6569
0.1226
1.3206




0.2463
0.1850
0.3964
1.3313
0.6213
0.4667
0.3284
0.7500
0.5633
1.2086





-------
                                           CANCER SITE:
                                                         Endocrine organs
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
1
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
••TH-VI' --,,-.-, n? Averaged 1st order rate.
Averaged 2nd order rate
"ri-v" AI • -f Avera^ed 1st order rate.
tvisK racio or . i ~ j .
Averaged 3rd order rate
"•«.; tit j f Averaged 2nd order rate
Averaged 3rd order rate'
Sum of weighted rates:

"U^-td l ri-k" nr-I- -f Avera8ed 1st order rate-
weigncea ristc ratio or „ , . , .
* Sum of weighted rates
-TT r-, , , , .i. .,.-.,. -f Averaged 2nd order rate
weigntea risK. ratio or _ , - , . :
Sum of weighted rates
nr» j. -, , . ,n _.^j f Averaged 3rd order rate
* Sum 01 weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females
0.2856
0.2768
0.2667
1.0318
1.0709
1.0379
0.2726

1.0477
1.0154
0.9784

X


0.2654
0.1577
0.1948
0.07~75
0.0366
0.0515
1.6829 | 2.1175
1.3624 1.5048
0.8095 0.7107
0.2091 0.0611
>
1.2692
0.7542
0.9316




1.2684
0.5990
0.8429




0.0213
0.0506
0.0473
0.4209
0.4503
1.0698
0.0373

0.5710
1.3566
1.2681





-------
                                           CANCER SITE: Bone
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis. )
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"nni-" rat-la of Avera2ed I" order rate:
.tvisK racjio or , — r — ^ * , :
Averaged 2nd order rate
"in it" rat I -f AvaraSed 1st order race.
K.ISIC ratio or . . . „ . . :
Averaged 3rd order rate
,,ric,r, , _ _f Averaged 2nd order rate
. K.XSK ratio or . - . _ . , :
'-eraged 3rd order rate
Sum of weighted rates:
..,T-i!rhrpd riEk.< tl f Averaged 1st order rate.
weixnueu J^ISK ratio or _ ^ . . , :
Sum of weighted rates
"U -lal rpj rick" r^ti- n.f Avera^ed 2nd order rate
weiftnueu ristc rauxo or ^ ^ t , . :
6 Sum of weighted rates
"u^-taht-arf r-icir" 1-^^^n nf Averaged 3rd order rate.
weisnteu nsK. ratio or — - _ . , , :
& Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
Ts greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
1.3432
1.4098
1.3313
0.9528
1.0089
1.0590
1.3424
1.0006
1.0502
0.9917


.
0.8044
0.8390
0.8824
0.9588
0.6826
6.5224
1.2662
0.1046
0.9116 0.5391
0.9508 5.1512
0.8581 . 1.2890
0.9374
0.9777
1.0283


X
0.5296
5.0600
0.9823



0.2112
0.2964
0.9172
0.7125
0.2303
0.3232
0.6137
0.3441
0.4830
1.4945




-------
                                               CANCER SITE:
                                                             Connective tissue
CZJ Urban and Rural Counties
\~~~2 Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ri-k" rania of Avera8ed lst order rate-
Averaged 2nd order rate
'"M -L-» ran - -f AveraSed 1st order rate.
Averaged 3rd order rate
•Ti-i-" --jit- -if AvaraSed 2nd order rate.
K.J.SK ratio 01 A j •> j j ^ •
Averaged 3rd order rate
Sum of weighted rs.tes:
"B I I r J i-I-t" r-ri- -f AveraSed lst order rate-
6 Sum of weighted rates
"U • it J i-L" I--MJ -f Avera«ed 2nd order rate.
Weighted i isk r^tio of gum Q£ weighted rates •
'"J I i r 1 rl-k" rail- f Averased 3rd order rate.
& Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
White White Non-white Non-white
Males Females Hales Females
0.5325
0.6488
0.6220
0.8207
0.8561
1.0431
0.6017
0.8850
1.0783
1.0337


0.3985
0.5463
0.4597
0.3774
0.7038
0.3767
0.7294 • 0.5362
j
0.8660 ; 1.0019
1.1884 ; 1.8683
0..4528
0.8801
1.2065
1.0152


0.3928
0.'9608
1,7917
0.9590


0.3912
0.2277
0.6883
1.7180
0.5684
0.3308
0.1530
2.5569
1.4482
4.4987


The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.

-------
                                                :ANCER SITE:
CQ  Urban  and  Rural  Counties

If  Rural  Counties (counties  having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged  2nd  order rate:
Averaged  3rd  order rate:
 !Risk"  ratio of
                Averaged 1st order rate.
                Averaged 2nd order rate'
„.,..„ „„.,„  ,: Averaged 1st order rate.
 Risk  ratio of	B—•:—T—	1	:
                Averaged 3rd order rate
"Risk" ratio of
                 Averaged 2nd order rate,
                 Averaged 3rd order rate'
 Sum  of weighted rates:
 "Weighted  risk"  ratio of Averaged 1st order rate;
                           Sum of weighted rates


 "Weighted  risk"  ratio of Averted 2nd order rate;
     6                      Sum of weighted rates


 "Weighted  risk"  ratio of Averaged 3rd order rate:
     0                      Sum of weighted rates
Trends

The  averaged  first order rate is greater
than the  second  order rate which in turn
is greater  than  the third order rate.

The  averaged  third order rate is greater
£han the  second  order rate which in turn
is greater  than  the first order rate.
                                                      White       White     Non-white    Non-white
                                                      Males      Females     Males       Females
2.2210


2.1782


2.2463


1.0196



0.9887



0.9697



2.2328
                                                     1.0060
                                                                 1.2231
                                                                 1.2260
                                                                 1.3215
                                                                             1.0202
                                                                             3.8701
                                                                             0.7334
                                                                 0.9976   !   0.2636
                                                                  0.9255
                                                                 0.9277
                                                                             1.3910
                                                                             5.2769
                                                                  1.2867   :   0.9986
                                                     0.9947   !   0.9506
                                                     0.9755   I   0.9528
            1.0270
                        1.0216
                        3.8755
                                                                             0.7344
0.5785


0.2143


0.7158


2.6995



0.8082



0.2994



0.6399


0.9040



0.3349



1.1186

-------
                                               CANCER SITE:  Lymphosarcoma
Si  Urban and Rural Counties

133  Rural Counties  (counties  having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
;'^i-k" ratio of Avera§ed lst order rate-
Averaged 2nd order rate'
„„ , , it . - Averaged 1st order rate
iCisK ratio or , , . i . .
Averaged 3rd -order rate
White White Non-white Non-white
Males Females Males Females
4.9200
4.7684
4.6771
1.0318
1.0519
\
..„.,.. . _ Averaged 2nd order rate
Risk ratio of Averaged 3rd Qrder rate-
Sura of weighted rates:
,,„ t .,.. , ,-,„ r,ri, _f Averaged 1st order rate.
weigntea riSK. ratio or „ - . , ,
s Sum of weighted rates
..„.,, i -i.. - tl- f Averaged 2nd order rate.
Weighted risk ratio of Sua of weighted ratfis -
„., . , . , ,„ .. ±. - Averaged 3rd order rate.
weigntea riSK ratio or. _ - . , ._ ,
e Sum ot weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate-
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
1.0195
4.7492
1.0360
1.0040
0.9848

X

3.2943
3.2386
3.2264
1.0172
11.3366
3.4169
1.4085
3.3178
1.0210 j 8.0487
•
1.0038 '; 2.4259
1
3.2350 | 5.4374
1.0152
0.9980
0.9943

X

2.0849
0.6284.
0.2590



1.1738
1.4381
1.2652
0.8162
0.9278

1.1367
1.2371
0.9488
1.16-25
1.0227




-------
                                                CANCER SITE:  Malignant Melanoma
CS  Urban and Rural Counties

|~~T  Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged  1st order rate:
Averaged  2nd  order rate:
Averaged  3rd  order rate:
"Risk"  ratio  of- Averaged  1st  order rate.
                Averaged  2nd  order rate
 "Risk"  ratio of Averted  1st order rate;
                Averaged  3rd order rate
"Risk"  ratio  of  Averaged  2nd  order rate;
                 Averaged  3rd  order rate
Sum of weighted  rates:
"Weighted  risk"  ratio  of  Averaged 1st order rate;
                           Sum of  weighted rates



"Weighted  risk"  ratio  of  Averaged 2nd order rate;
                           Sum of  weighted rates



"Weighted  risk"  ratio  of  Averaged 3rd order ratE;
                           Sum of  weighted rates
Trends


The averaged  first  order  rate  is  greater
than the  second  order  rate  which  in turn
is greater  than  the third order rate.


The averaged  third  order  rate  is  greater
.than the  second  order  rate  which  in turn
is greater  than  the first order rate.
                                                      White       White     Non-white   Non-white
                                                      Males      Females      Males      Females
1.6870



1.7778



1.7174



0.9489



0.9822



1.0352




1.7157
                                                                  1.3077
                                                                  1.1799
                                                                  1.1641
1.1234
1.0136
1.2024
0.9833   i   1.0876
1.0362   j   0.9813
1.0010
0.9681
            1.3904
            1.8585
            1.5248
1.1083      0.7481
0.9119
1.2188
1.4877
            0.9346
            1.2492
1.0249
            1.8647
            0.7126
            1.9614
            2.6169
0.9507
0.3633
1.8684
            0.9980
            0.3814
1.0498

-------
CANCER SITE:
             Leukemia
223 Urban and Rural Counties
[~ Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Iti-lt" ratio of Avera8ed lst order rate-
Averaged 2nd order rate
HTM-I-H ~=rir< *f Averaged 1st order rate.
K.ISK ratio or ~ : ^r : r .
Averaged 3rd order rate
itn_r i H _-^j ^ Averaged 2nd order rate
&isK ratio or *r : ^ ~. . \
Averaged 3rd order rate
Sum of weighted rates:
,,,, _, ^ _, _^ , ,, tj = Averaged 1st order rate
Weighted rxSK ratio or ^ 	 ^~r 	 : — , , 	 ' 	 :
Sum or weighted rates
'"j -slr^J r'-k" Art- -f Avera§ed 2^d order rate.
neigntea risK ratio or _ - , , , :
Sum or weighted rates
•"J-lalt-d rl k" M f AveraS£d 3rd order rate.
weignupu risK racio or. r ^ ~ T ^ .
Sum of weighted rates
Treads
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.


White
Males
8.5566
8.6695
8.9543
0.9870

0.9556

0.9682

8.8224
0.9699

0.9827

1.0149



X
t

White
Females
5.5781
5.6443
5.8889
0.9883

0.9472

0.9585

5.7848
0.9643

0.9757

.1.0180



K


Non-white
Males
7.1985
8.4316
5.5888
.
0.8527

1.2225

1.4319

6.3637
1.1312

1.3249

0.3782






Non-white
Females
2.7852
2.0768
2.9523
1.3411

0.9434

0.7034

2.8482
0.9779

0.7292

1.0365






-------
CANCER
SITE- ICD'S not listed
P(J Urban and Rural Counties
O Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis . )
White White Non-white Non-white
Hales Females Hales Females
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:

"Hl-k" ratio of Averaged 1st .order rate.
Averaged 2nd order rate
n«.t ,n ^., f Averaged 1st order rate
KISK ratio Ot . — : — r — ; 	 I
Averaged 3rd order rate
\\-r-j in j f Averaged 2nd order rate
Averaged 3rd order rate'
Sum of weighted rates:
i.,,plEhrpJ ri-v.. r r± f Averaged 1st order rate.
weignr.eu nsK rauio 01 -, ,. : , . r
• Sum of weighted rates
"U-ial -r-J ii£k" -nti- Qf Avera8ed 2nd order rate-
weixnceu i ISK ratio ot « ^ .. . , :
Sum or weighted rates
"U -'2l r-J rifik" rati- -f Avera8ed 3rd order rate
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
:than the second order rate which in turn
is greater than the first order rate.



9.8353
9.5067
9.6726

1.0346
1.0168
0^9828

9.6976
1.0142
0.9803
0.9974






9.4042
9.1145
9.4163
10.1293
5.0741
13.7322
i
1.0318
1.9963
0.9981 0.7376
0.9679 0.3695

0.3828 11.8870
1.0023
0.9714
1.0036






i
i
i
i
1
I
i .
1
0.8521
0.4269
1.1552










12.4898
7.6560
1.3590

1.6314
1.3345
0.8180

10.5081
1.1886
0.1286
0.8906











-------
                                                CANCER SITE*
                                                                           neoplasms
    Urban  and  Rural Counties
\~~r  Rural  Counties  (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged  1st order rate:
Averaged  2nd order rate:
Averaged 3rd order rate:
 "Risk"  ratio of- Averaged 1st order rate;
                 Averaged 2nd order rate•
                                                      White
                                                      Males
  Risk"  ratio of
Averaged 1st order rate.
Averaged 3rd order rate'
 „„.  . ,,     .    , Averaged 2nd order rate
  Risk  ratio of Averajed ,rd order race:
 Sum of  weighted rates:
 •I,. .  .   .   j  , ii    •    s Averaged 1st order rate
 "Weighted  risk  ratio of —	*-:	.  ...  	:
                           sum of weighted rates

 ,,., .  .   ,   .  , „    .    e Averaged 2nd order rate
 "Weighted  risk  ratio of —	°-^	.  .   ;	:
     0                     Sum of weighted rates


 M,. ,  ,      •  , .1    *    * Averaged 3rd order rate
 "Weighted  risk  ratio of.-r	°~z	:~T—2	;
     0                     Sum of weighted rates
 Trends

 The averaged first order r.ate is greater
 than the second order rate which in turn
 is greater than the third order rate.

 The averaged third order rate is greater
 than the second order rate which in turn
 is greater than the .first order rate.
                                                 White
                                                Females
Non-white
  Males
Non-white
 Females
169. 290.
161.980
154.326
1.0494
1.1015
1.0496
159.155
1.0681
1.0177
0.9697
X


131.514
127.931
127.666
1.0280
1.0301
1.0021
128.677
1.0220
0.9942
0.9921
X


149.829
140.887
127.847
1.0635
1.0869
1.0220
142.739
1.0497
0.9870
0.9657



133.907
148.075
109.866
0.9043
1.2188
1.3478
120.956
1.1071
1.2242
0.9083




-------
                                                CANCER SITE:
                                                           .  Lip
    Urban and Rural Counties
133  Rural  Counties (counties having urban
    centers  with  100,000 inhabitants are
    excluded from anal7sis.)
Averaged  1st order rate:
Averaged  2nd order rate:
Averaged  3rd  order rate:
 "Risk"  ratio of Averaged 1st order rate.
                 Averaged 2nd order rate
 Risk"  ratio of
Averaged 1st order rate.
Averageu 3rd order rate'
 "D-!  i,"     •<    - Averaged 2nd order rate
  Ris*   ratio or Averaged 3rd Qrder     =
 Sum of  weighted rates:
 "Weighted  risk" ratio of Averaged 1st order rate;
     &                      Sum of weighted rates

 .... , ,   ... it    .    - Averaged 2nd order rate
 Weighted  risk  ratio or —	=-?	r-r—~,	'
     6                      Sum or weighted rates


 ,,., . ,   ,   .  , H    .    r Averaged 3rd order rate
 Weighted  risk  ratio of —	°-^	—	j	:
     °                      Sum of weignted rates
Trends

The  averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.

The  averaged third order rate is greater
jihan the second order rate which in turn
is greater than the first order rate.
                                                      White       White     Non-white   Non-white
                                                      Males      Females      Males      Females
                                     0.3513
                                     0.3959
                                     0.3771
                                     0.877
                                                      0.932
                                     1.050
                                     0.3765
0.0095
0.0144
0.0292
0.660
0.325
0.493
0.0246
                                     0.9331   !   0.3862
                                     1.0515   i   0.5854
                                     1.0020
1.1870
0.2849
0.2044
1.3938

-------
                                           CANCER SITE:  Saliva^ Glands
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)

Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"TM-v" ratin of Avera§ed lst order rate-
AloK L&LJ.O Oi ~ ; r : 	 ; .
Averaged 2nd order rate
n«,_k,i tl. f Averaged 1st order rate.
.\ISK racio or ~ —
Averaged 3rd order rate
ii-Mct-ii ,--,,--!- -,f Averaged 2nd order rate.
Risk ratio or •. • •: — - — 	 	 	 	 :
Averaged 3rd order rate
Sum of weighted rates:
"•J^iffVr-i r-T-t" rarj- -f Averased 1st order rate.
weigncea risic ratio 01 _ ", . , .
Sum of weighted rates
'"J-iB- r^J -1-k" r^r-I- -f AveraSed 2nd order rate-
weix.iceu risK racio 01 t; ^ ; ; : .
6 Sum of weighted rates
...,, isvr-, r,-,i-» raM- -f Averaged 3rd order rate.
iJeigfited ristc ratio or _ "- • . , ,
Sum or weighted rates
Trends
The averaged first order rate is greater
than the second order rate- which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.

White
Males
0.4189
o T?cn
\J • J JVJ.J
0.3952
1.268

1.060
0.836

0.3890

1.0769

0.8491

1.0159




White
Females
0.2349
f) 1 QSQ
u . J.J jy
0.2213
1.200

1.130
0.885

0.2194

1.0106

0.8929

1.0086




Non-whi te
Males
0.0732
0,2094

0.350


0.1600

0.4575



1.3088




Non-white
Females
0.0780
fl fifi?6!
\t • o o / j
0.3231
0.113

0.241
2.128

0.3435

0.2271

2.0014

0.9406





-------
                                                CANCER SITE:  Nasopharynx
L~T  Urban and Rural Counties

QT]  Rural Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Hirik" ratio nf Averaf?ed 1st order rate.
Averaged 2nd order rate
•Tifit" rit • f Averased 1st order rate.
Kistc ratio or — , ~ — . . :
Averaged 3rd order rate
,.r.clrn _,.-,- -f Averaged 2nd order rate.
KISK ratio or . — . _ • , :
Averaged 3rd order rate
Sum of weighted rates:
"Uaiohtad risk" ratio of AveraSed lst order rate-
weignLeQ risic ratio or ^ ^ ;^ :^ ; .
Sum of weighted rates
"U'aiaht^d ri-k" ratij of Avera§ed 2nd order rate
Sum of weighted rates
"U^icl -rM r-f^" ratia -f AveraSed 3rd order rate.
weignteu risK ratio or ~ _ . , , :
0 Sum or weighted rates
•Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
•The averaged third order rate is greater
-than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Hales Females Males Females
0.2483
0.3191
0.2683
0.778
0.925
1.189
0.2730
0.9095
1.1689
0.9828




0.1020
0.0939
0.0815
1.086
1.252
1.152
0.0858
1.1888
1.0944
0.9499

X


0-.1988
0.0403
0.0656
4.933
3.030
0.614
0.0796
2.4975
0.5063
0.8241




0.0232
	 	
0.0248

0.935

0.0210
1.1048

1.1810





-------
                                                CANCER SITE:  T°ngue and M°Uth
r~T  Urban  and  Rural Counties

QTT  Rural  Counties  (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
t.pj .« fj f Averaged 1st order rate.
Averaged 2nd order rate'
„ ,„ . ... Averaged 1st order rate.
Averaged 3rd order rate'
„.. ,„ . _- Averaged 2nd order rate
Risk ratio of Averaged 3rd Qrder rat(,.
Sum of weighted rates:
"u i -• i -r-i" -t f Averaged 1st order rate.
°a Sum of weighted rates
	 , . i • i " r,r-f- -f Averaged 2nd order rate.
Weighted risk ratio of Sm Q£ weighted rates -
	 , i -r k" r-Li- -f Avera8ed 3rd order rate
rte-_2i*teci rxsK racio or _ - , r . .
* Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than, the second order rate which in turn
is greater than the .first order rate.
White White Non-white
Males Females Males
3.5841
3.2118
2.8988
1.116
1.236
1.108
3.034
1.1827
1.0599
0.9566

X

0.9064
0.8769
0,7178
1.034
1.263
1.222
; 0.7636
1.1870
1.1484
0.9400

X

0.6546
1.6276
4.3316
0.402
0.151
0.376
3.4376
0.1904
0.4.735
1.2601


X
Non-white
Females
0.3131
0.5354
1.6891
0.585
0.185
0.317
1.3466
0.2325
0.3976
1.2543




-------
                                                CANCER SITE:  Esophagus
CU Urban  and  Rural Counties

l3{7 Rural  Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
White White Non-white Non-white
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"IU-1-" ratio of Averased 1st order rate.
.XVXoK iciUlU OIL — ~ A , , »
Averaged 2nd order rate
"risk" i-ii-i- -f Averased 1st order rate.
KU.SK rauio 01 ~ . _ . . .
Averaged 3rd order rate
'Ti.sU1 i-ari- -f AveraB£d 2nd order rate
AJ.SK. rauiLO ot , « . . — •
Averaged 3rd order rate
Sum of weighted rates:
"Urlahtrd rl-lc11 rati" af Avera8ed lst Order rate -
weignued nstc rauxo OL T ^ ; r . .
Sum of weighted rates
"XJMsl-r- 1 r-Uk" i-jr-I- -f AveraS£d 2nd order rate-
weigncea risK. ratio or ;; ~: ; r~ — ; 	 :
iium of weignted rates
"U^iahfl ri£k" ran- -f AveraSed 3rd order rate.
wexgriutru LJ.SK. raczo or z ^ ^ ; :^
Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
.than the second order rate which in turn
is greater than the first order rate.

Males Females Males Females
4.0135
3.1768
2.8908
1.263
1.388
1.100
3.0741
1.3056
1.0334
0.9404

X


i
i
0.9776
0.7710
0.7367
1.268
1.327
1.046
0.7715
1.2671
0.9994
0.9549

X



717575
4.3694
5.4566
1.775
1.422
0.801
5.6010
1.5484
0.8721
1.0290





0.7111
27.4450
0.5824
0.026
1.221
47.124
4.4352
0.1603
6.1880
0.1313






-------
                                                CANCER SITE:
                                                              S totnach
•[  Urban  and  Rural Counties

123  Rural  Counties  (counties having urban
    centers  with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"n-k" ratio of Avera§ed lsc order rate-
Averaged 2nd order rate
'-I-U1 rati- -f Avera§ed lst order rate
KXSK ratxo 01 T^ ; ~ t ,, .
Averaged 3rd order rate
"-Ik" 1-j.ri- -f AveraSed 2nd order rate
Risk ratio of Averaged 3rd OJ_der rat£.
Sum of weighted rates:
,,y .3Vf_l1 r:i_kM ...,- f Averaged 1st order rate.
weixncea rxsic ratio or ., •. • :
* Sum of weighted rates
"U r-'T-l -l-L" r rij -f Avera8ed 2nd order rate-
wej-gnteci rxsK. racio or _ ,. . , 3 • :
0 Sum of weighted rates
"U 'ltd --L" Mti- -f Avera8ed 3rd order rate
wexKuu€*Q rxsic racxo QL „ ,. . , . .
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.
White White Non-white Non-white
Males Females Males Females
19.3141
16.6741
14.2376
1.158
1.356
1.171
15.2290
1.2682
1.0949
0.9349

X

0.2747
7.6514
7.4825
1.212
1.240
1.022
7.7290
1.2000
0.9900
0.9680

X

24.0850
19.6415
12.9416
1.226
1.861
1.518
15.4279
1.5611
1.2731
0.8388

X

8.5759
14.9487
6.4664
0.574
1.326
2.312
7.9503
1.0787
1.8803
0.8134




-------
                                           CANCER
SITE-   LarSe  Jntestine
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Rink" ratio of Avera8ed 1st order rate.
Averaged 2nd order rate
"Plat11 rani- -f Avera^ed lst order rate-
Averaged 3rd order rate
"ric.k" ,.111- if Averased 2nd order rate.
AISK ratio or ~ — — : r . .
Averaged 3rd order rate
Sum of weighted rates:
"XJ itthr -j ri-k" rati- of Avera8ed lst order rate-
weignceu risic rauio or - e ; , . .
& Sum of weighted rates
"U '-I i I v-I-V" ,-ai-I -f AveraSed 2nd order rate-
weigntea risK ratio or „ . . , . ,
0 Sum or weighted rates
"U U1 i 1 -i-k" r^t-1 - f Avera^ed 3rd order rate.
Weighted risk ratio of Sum of weighted rates •
Trend's
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
"than the second order rate which in turn
TTs greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
16.0514
15.8353
15.6321
i.014
1.027
1.013
15.7143
1.0214
1.0077
0.9948

X

16.4708
16.4945
16.8474
0.998
0.978
0.9.79
16.7508
0.9833
0.9847
1.0058


X
8-. 9 946
10.9660
10.9501
0.820
0.821
1.001
10.6917
0.8413
1.0256
1.0242



16.1056
8.8441
15.4474
1.821
1.043
0.572
14.5894
1.1039
0.6062
1.0588




-------
                                               CANCER  SITE:
                                                             Rectum
ir  Urban  and Rural  Counties

j_T7  Rural  Counties  (counties  having  urban
    centers with 100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"IM-k" ratio of Avera8ed lst order rate -
.IN.ISK caLiu oj. — - - : :
Averaged 2nd order rate
,,.. .,,, ... .f Averaged 1st order rate
Averaged 3rd order rate
"n-k" ~aci- of Averaged 2nd order rate
Averaged 3rd order rate'
Sum of weighted rates:
'"J laii--^ ri-k" r^r-ia -f AveraSed lst order rats-
weigncsa risK racio or ., , , . .
6 Sum of weighted rates
.... •--, i ,-fM-t. raM- -f Averaged 2nd order rate
weigntea risic ratio or „ , . . :
e Sum of weighted rate;;
"U --• • r 1 isk." mti- ^f Averaged 3rd order rate.
weisncpu ristc racio or ^ ^ i \ »
* Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater' than the .first order rate.

White White Non-white Non-white
Males Females Males Females
7.3843
7.7999
7.1967
0.947
1.026
1.084
7.3063
1.0107
1.0676
0.9850


I
4.6759
5.0243
4.6700
0.931
1.001
1.076
4.7207
0.9905
1.0643
0.9893



1.5363
0.5218
4.99.40
0.161
0.308
1.907
5.2092
0.6509
1.8279
0.9587



2.6566
1.4953
3-. 0834
1.777
0.862
0.485
2.8014
0.9483
0.5338
1.1007




-------
                                               CANCER SITE:
                                                             Liver
EU Urban  and Rural  Counties

|*J Rural  Counties  (counties having urban
    centers with. 100,000  inhabitants are
    excluded from analysis.)
White White Non-white Non-white
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ri~k" ti £ Averaged 1st order rate.
Averaged 2nd order rate"
»„_, , n . , ,. Averaged 1st order rate
KJ.SK ratio or . : — ; — : 	 . :
Averaged 3rd order rate
"ri L" - - i • - f AveraSfid 2nd order rate
Averaged 3rd order rate
Sum of weighted rates:
"U-ltl-t-J risk" rati- Jf Averaged lst order rate-
0 Sum of weighted rates
"U i 1-r-l ri 1." ran- cf AveraSed 2nd order rate -
wei:!ntec risK ratio or •* ,
Sum or weighted rates
«r, ^ ^^ j • , .i _j . r Averaged 3rd order rate
weiizhteci nsK ratio 01 — r 	 ~: : ; : 	 — :
Sum or weighted rates
Trend's
The averaged first order rate is greater
than the second order rate which in turn
is greater than che third order rate.
The averaged third order rate is gre'ater
j:han the second order rate which in turn
is greater than the first order rate.
Males Females Males Females
5.1124
4.6267
4.7725
1. 105
1.071
0.969
4.7950
1.0662
0.9649
0.9953




6.5919
6.0335
6.2999
1.092
1.046
0.958
6.2986
1.0466
0.9579
1.0002




6'. 2985
5.7708
5.5268
1.091
1.140
1.044
4.6661
1.1116
1.0185
0.9754

X


6.1691
5.0352
4.8811
1.225
1.264
1.032.
5.0695
1.2169
0.9932
0.9628





-------
CANCER SITE:
             Pancreas
~~] Urban and Rural Counties
|Xj Rural Counties (counties having urban
centers with 100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
„-,,,, .. . M . _f Averaged 1st order rate.
Risk ratio of Averaged 2nd order rate-
ii_. ,n . f Averaged 1st order rate.
" s "a Averaged 3rd order rate'
„. ,. , Averaged 2nd order rate.
&3.SK ratio or Averaged 3rd ordar rate-
Sum of weighted rates:
"U 1 1 i 1 -'-V11 "Ati- c.f Avera§ed lst order rate
Weighted risk ratio of Sum Qf weighted rates .
„ . . .,...,, r- -r Averaged 2nd order rate
Weignted risk ratio of Sum Qf welghted rates .
,.., ... , .,„.__,. Averaged 3rd order rate
Weighted risk ratio of. Sum Qf weighced rates :
Trends
The averaged first order rate is greater
than the second order rate 'which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater' than the .first order rate.
White White Non-white Non-white
Males Temales Males Females
9.1222
8.8440
8.4038
1.031
1.085
1.052
8.5577
1.0660
1.0334
0.9820

X

6.0195
5.4865
5.4293
1.097
1.109
1.010
5.5111
1.0922
0.9955
0.9852

X

2.8699
5.1862
7.5443
0.553
0.380
0.687
6.5689
0.4369
0.7895
1.1485


X
11.4348
2.6449
3.7939
4.323
3.014
0.697
4.6169
0.0874
0.5729
0.8217




-------
                                               CANCER SITE: Nose and Middle Ear
D  Urban  and  Rural  Counties

133  Rural  Counties  (counties having  urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
uri3kt, tl f Averaged 1st order rate.
Averaged 2nd order rate
•Ti-t" r-c'- f Averased 1st order rate.
Averaged 3rd order rate
"n -t" i--ti- f Averased 2nd order rate.
Risk ratio of Averaged 3rd Qrder rate-
Sum of weighted rates:
"TTplBht&d risk" ratio of Avera8ed lst order rate-
Sum of weighted rates
"U-ial-r-J rl-t" rati- c.f Avera8ed 2nd order rate
wej.gnt.su risic rano 01 z ^e ^ , . .
Sum of weighted rates
"U -1al r 1 r-i^-" rarii cf Avera8ed 3rd order rate -
Weighted risk ratio or s^ Q£ w£ighted rates -
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
Ts greater than the first order rate.
White White Non-white Non-white
Males Females Hales Females
0.3687
0.5410
0.4433
0.682
0.832
1.220
0.4477
0.8235
1.2084
0.9902



0.2280
0.1748
0.2276
1.304
1.002
0.768
0.2202
1.0354
0.7938
1.0336



0-.3242
0.2900
0.2350
1.118
1.380
1.234
0.2551
1.2709
1.1368
0.9212

X

0.4860
0.2206
0.1769
2.203
2.747
1.247
0..2231
2.1784
0.9888
0.7929




-------
                                               CANCER SITE:
                                                             Larynx
~~y  Urban and Rural Counties

\T.  Rural Counties  (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
•"•I-k" ratia of Averase
-------
                                               CANCER SITE: Trachea> Bronchus  and Lung
CU Urban  and Rural  Counties

Qj Rural  Counties  (counties having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
••TM-J," r,tl, af Averaged 1st order rate.
KXSK r&ulO OZ ~ : r • .
Averaged 2nd order rate
,,r. , „ - Averaged 1st order rate
Averaged 3rd order rate'
„_. ,„ . _f Averaged 2nd order rate.
Risk ratio of Averaged 3rd order race-
Sum of weighted rates:
"U d -i k" ri f Avera8ed 1st order rate.
Sum of weighted rates
"U I 1 t 1 i-l -k" rarl- cf AveraSed 2nd order rate-
weiRrmeci rxsK. rncxo 01 r ^ . . . *
6 Sum of weighted rates
"U-iclt^J risk" r-ti- 3f Avera2ed 3rd order rate
tvexxncea rxsK ratio oz — ^ . , t
Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
-ehan the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Hales Females
31.3235
30.0740
28.0289
1.042
1.118
1.073
28.7382
1.0900
1.0465
0.9753

X


4.9250
5.0627
5.0978
0.973
0.966
0.993
5.0714
0.9-711
0.9912
1.0052


X

26.4475
19.0065
26.6694
1.323
0.992
0.750
25.6432
1.0314
0.7798
1.0400




5.3389
2.9137
4.9340
1.832
1.082
0.590
4.6978
1.1365
0.6202
1.0503





-------
                                               CANCER SITE:
                                                            Breast
•r  Urban and Xural Counties

HT  Rural Counties  (counties having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
»r--k" --Jtio -f Avera8ed lst order rate-
RISK ratio on . ; r r , •
Averaged 2nd order rate
,,...,. . . - Averaged 1st order rate.
Risk ratio of Averaged 3rd Qrder rate-
... „ , Averaged 2nd order rate.
Risk ratio of Averaggd 3rd Qrder rat(,.
Sum of weighted rates:
,. . . ....,„,. _f Averaged 1st order rate.
Weighted risk ratio of s^ Qf weighted rates •
...... , . ,„ -,-,- -f Averaged 2nd order rate.
Weighted risk ratio of S(jm Qf weighted rates •
...... . ,.. , . r Averaged 3rd order rate.
Weighted risk ratio of Sum of weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than ;he second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females
0.2628
0.1778
0.2389
1.478
1.100
0.74-''
0.23:; 3
1.12t4
0.7621
1.0240



i
25.0697
24.9366
24.0034
1.005
1.044
1.039
24.2674
1.0331
1.0276
0.9891

X


0.2047
1.8365
0.2149
o.iii
0.952
8.546
0.4557
0.4492
4.0301
0.4716




22.5271
28.2036
17.0904
0.799
1.318
1.650
19.3798
1.1624
1.4553
0.8819





-------
                                               CANCER SITE:
                                                             Cervix Uteri
    Urban and Rural Counties
    Rural Counties  (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of- Averaged 1st order rate.
                Averaged 2nd order rate
 Risk" ratio of
""
 Risk" ratio of
Averaged 1st order rate.
Averaged 3rd order rate'


Averaged -2nd order rate.
Averaged 3rd order rate'
Sum of weighted rates:
„.,  . ,    ,   . , ,,     .    .. Averaged  1st order  rate
 Weighted risk  ratio of —=	^	——3	:
                          Sum of weighted rates


it,,  . .    j   * i ti     .    .c Averaged  2nd order  rate
 Weighted risk  ratio of —z	 f	r r- •.	'
    0                     Sum of weighted rates


„.,  ..,.,!i     .    , Averaged  3rd order  rate
 Weighted risk  ratio of —r	°-z	7—-—-:	:
    6                     Sum of weighted rates
Trends

The averaged first order  rate  is  greater
than the second order rate which  in  turn
is greater  than the  third order rate.

The averaged third order  rate  is  greater
than the second order rate which  in  turn
is greater  than the  first order rate.
White White Non-white Non-white
Males Females Males Females










8.1718
7.7446
7.9294
1.055
1.030
0.977
7.9335
1.0300
0.9762
0.9995










14.5349
23.4777
9.1016
0.619
1.507
2.580
11.8566
1.2259
1.9801
0.7676

-------
                                                CANCER SITE:
                                                             Corpus Uteri
r~I  Urban  and Rural  Counties

L*3  Rural  Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"n1clr«i riMr, nf Averaged 1st order rate.
Averaged 2nd order rate
'"M-k" ratio af Avera&ed lst order rate-
AXSK rauxo 01 L : r •
Averaged 3rd order rate
..Tr-t.i rl. -f Averaged 2nd order rate.
AISK ratio or , _, _ , ,
Averaged 3rd order rate
Sum of weighted rates:
"U lE'iraJ ri-k" rati- -f Avera*ed lst order rate-
wexKnceu LJ.SK. raua.o or r ^: ; : , «
Sum of weighted rates
"W -nl-t-d ri-k" rari- -f Averaged 2nd order rate_
Sum of weighted rates
"u tr-t d ri-k" r^ri- -f Averased 3rd order rate.
ne.Lx.tu?u nsK rauiuo or _ ,. . r ^
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.

White White Non-white Non-white
Males Females Males Females














6.5272
6.8642
6.9039
0.951
0.945
0.994
6.8515
0.9527
1.0018
1.0076


X















11/6947
10.4828
8.9368
1.116
1 309
1.173
0.5139
1.2292
1.1018
0.9393





-------
                                                CANCER SITE:   Ovary and Fall°Pian tubes
EZJ  Urban and Rural Counties

pTf  Rural Counties (counties having urban
     centers with  100,000 inhabitants are
     excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ri-t" ratio of Avera8ed lst order rate-
.RISK ratio 01 , : T r ^ •
Averaged 2nd order rate
"n-L" YATI f AveraSed 1st order rate.
RISK racio 01 . : r , , .
Averaged 3rd order rate
•TitL" I-AI-I- -f Avera&ed 2nd order rate
jxxSiC rac.LO or . r r ; . :
Averaged 3rd order rate
Sum of weighted rates:
"Uficht-Ld ri-t" ratia -f Avera8ed lst order rate-
Sum of weighted rates
"U 'a' i J riqk" ratio cf AveraSed 2nd order rate.
0 Sum of weighted rates
"U--tBl-r--1 ritl;" -aria -f Avera8ed 3rd order rate
weignteu risK rario 01 _ . , ,
e Sum or weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order race is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females












8.0938
8.5943
8.3943
0.942
0.964
1.024
8.3852
0.9652
1.0249
1.0011


i













4.2110
5.7223
6.2791
0.736
0.671
0.911
5.9324
0.7098
0.9646
1.0584




-------
                                                CANCER SITE:
                                                             Prostate
if  Drban  and  Rural  Counties

l23  Rural  Counties  (counties having urban
    centers with   100,000 inhabitants are
    excluded from analysis.)
Averaged  1st  order  rate:
 Averaged 2nd order rate:
Averaged  3rd  order rate:
 "Risk"  ratio of Averaged 1st order rate;
                 Averaged 2nd order rate
  Risk"  ratio of
  Risk"  ratio of
Averaged 1st order rate.
Averaged 3rd order rate'
Averaged 2nd order rate.
Averaged 3rd order rate'
 Sum of weighted rates:
 it,, .  .   .   _,  i ii   ..-    c Averaged 1st order rate
  Weighted  risk  ratio of —	«-T	.  , •	
     0                     Sum of weighted
                          rates
 "Weighted risk" ratio of Averaged 2nd order rate
     6                     Sum of weighted rates


 ,„, ,, .   ,  .  , >.    .    ,- Averaged 3rd order rate
  Weignted risk  ratio of —5	=7	•     	:
     0                     Sum of weighted rates
 Trends

 The averaged first order rate is greater
 than the second order rate which in turn
 is greater than the third order rate.

 The averaged third order rate is greater
 than the second order rate which in turn
 is greater than the .first order rate.
White White Non-white Non-white
Males Females Males Females
18.2882
18.5279
18.3156
0.987
0.998
1.012
18.3423
0.9970
1.0101
0.9985










15.0168
14.7194
21.4893
1.V020
0.699
0.685
19.6153
0.7656
0.7504
1.0955











-------
                                               CANCER SITE: Testis
    Urban and Rural Counties
    Rural Counties (counties having urban

    centers with  100,000 inhabitants are

    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of Averaged 1st order rate.

                Averaged 2nd order rate




"Risk" ratio of Averaged 1st order rate;

                Averaged 3rd order rate
 Risk" ratio of
Averaged 2nd order rate.

Averaged 3rd order rate'
Sum of weighted rates:
„,,  . .    ,   . , it     .   * Averaged 1st order rate
 Weighted risk  ratio of —	=^	——-,	
                          Sum of weighted rates




„,,  . .    ,   . . „     .   , Averaged 2nd order rate
 Weighted risk  ratio of -7	°-z	—r—-	:
    e                     Sum of weighted rates




it,, ., .    .   • , .i     j   c Averaged 3rd order rate
 Weighted risk  ratio of —5	°-:	——	
    0                     Sum of weighted rates
Trends



The averaged first order rate is greater

than the second order rate which in turn

is greater than the third order rate.



The averaged third order rate is greater

than the second order rate which in turn

is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
0.9430
0.8982
0.9220
1.050
1.023
0.974
0.9213
1.0236
0.9749
1.0008
i











0.0286
0.0236
0.1907
1.213
0.150
0.124
0.1441
0.1984
0.1638
1.3232













-------
                                           CANC£R SITE:
                                                        Kidney
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ri-v« ratio of Avera§ed lst order rate-
.RISK rauio t/i. ~ ~ ~ ; . .
Averaged 2nd order rate
,,*... .,„ r1. f Averaged 1st order rate.
Risk .atio of Averagfid 3rd order rate-
"-; L" -JM- if Avera8ed 2nd order rate
Risk ratio of Averaged 3rd order race-
Sum, of weighted rates:
,,„ . ... , ±.,.M M. .f Averaged 1st order rate.
Weignted risk ratio of ^ of weighted rat£S .
..„ . , , . , it . , Averaged 2nd order rate
neiLguteci risic racio or ^ >: ^ r t
6 . Sum of weighted rates
„„ . , , • ,»,--,,, F Averaged 3rd order rate.
Weighted risk ratio of Sum Qf weighted races -
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.
White White Non-white Non-white
Males Females Males Females
4.0716
3.5932
3.7473
1.133
1.086
0.959
3.7666
1.0810
0.9540
0.9949


2.1718
2.0285
2.0740
1.071
1.047
0.978
2.0796
1.0443
0.9754
0.9973


1.5582
2.3489
2.2447
0.663
0.694
1.046
2.1683
0.7185
1.0830
1.0350


0.3425
0.8401
1.7412
0.408
0.196
0.482
1.4318
0:2392
0.5867
1.2161



-------
                                               CANCER SITE: Bladder
d  Urban  and Rural  Counties

|TT  Rural  Counties  (counties  having urban
    centers with  100,000  inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Hi -It" ratio of Avera8fid lst order rate -
.K1SK raulO Or — ~ . ,
Averaged 2nd order rate
"itr L" id -f Averased 1st order rate.
KiSK ratio ot — j - . . :
Averaged 3rd order rate
"ri^L" iiLi f AverflSed 2nd order rate
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:
"Uplehr^d ri^" ratio of Avera8ed lst order rate
wexgnceo riLSK rauxo or ~z ^ * . , .
Sum of weighted rates
"U--U1 i J ri-1" ratio c^ Avera^ed 2nd order rate.
wej-Kuteu riSi. r3.Cj.O 0*. ^ ^: ; ; ;
6 Sum of weighted rates
"U lit J ri-k" ratio -f Avera*ed 3rd order rate
° Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
6.6565
7.2752
5.7923
0.915
1.150
1.256
6.1129
1.0889
1.1901
0.9476


2.4786
2.27.22
2.1219
1.091
1.168
1.071
2.1874
1.1331
1.0388
0.9700
X

4.7320
5.2706
2.4443
0.898
1.936
2.156
3.1714
1.4921
1.6619
0.7707


1.6780
1.8197
2.6279
0.922
0.638
0.692
2.3898
0.7022
0.7614
1.0996



-------
                                                CANCER SITE:
                                                             Skin Melanoma
d   Crban and  Rural Counties

|1CT   Rural Counties  (counties  having urban
     centers  with  100,000 inhabitants are
     excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged' 3rd order rate:
••n-t^k'« ^-in nf Averaged 1st order rate.
rvistc ratio 01 — 	 - - 	 .
Averaged 2nd order rate
""I L-" url- rf Avera&ed lst order rate-
Risk ratio of Averaged 3rd order ratfi.
„ „ . , Averaged 2nd order rate
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:
"ut.tr -i -Heir" riri-t -if Averaged 1st order rate.
Weighted risk ratio or — - 	 ~ 	 ; r — j 	 :
6 Sum of weighted rates
....... . ,,, , ,f Averaged 2nd order rate
Weigated risk ratio of Suffl Q£ weightfid rat3S -
„ , . . , „ . . . Averaged 3rd order rate.
Weighted risk ratio of ^ Qf weighted rates •
Treads
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.
White White Non-white Non-white
Males Females Males Females
Q.9310
0.9973
1.2169
0.0934
0.765
0.820
1.1493
0.8100
0.8677
1.0588


X
0.7607
0.9125
0.9832
0,834
0.774
0.928
0.9456
0.8045
0.9650
1.0398


X
0.0126
0.0284
0.0651
0.444
0.192
0.432
0.0531
0.2373
0.5348
1.2373


X
	
0. 3851
0.3058


1.259
0.2776

1.387-2.
1.1016




-------
                                           CANCER SITE:
                                                         Other  Skin
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
,.rl-kr, rj *. Averaged 1st order rate.
KISK racio o— ~ . ; 	 .
Averaged 2nd order rate
"TM L" AM " Averaged 1st order rate.
K.ISK. ratio or . . „ . . :
Averaged 3rd order rate
"TM^" .in f Avera
-------
                                           CANCER SITE:
                                                         Eye
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
White White Non-white Non-white
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
IITM-I.I« -r^nn nf Averaged 1st order rate.
RISK ratio or 	 	 a — - — - — 	 	 	 	 :
Averaged 2nd order rate
"•neb" -r^i-t^ -,f Averaged 1st order rate.
Risk ratio of - 	 a — : — : — •: 	 -; 	 :
Averaged 3rd order rate
„ ,„ , Averaged 2nd order rate.
Averaged 3rd ordet rate'
Sum of weighted rates:
..„.,. , . , , .. .... £ Averaged 1st order rate
wexguceu rxsK racio ox z - . , .
6 Sum of weighted rates
„„ . , , . , n . £ Averaged 2nd order rate
weisnceu rxsK rsno ox, ,-, £ . T^ . 	 .
6 Sum of w€-.ighted rates
„„ .... , . .,„ (1 f Averaged 3rd order rate
weignteu nsK ratio or „ ,- , , , :
0 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.
Males Females Males Females
0.1859
0.2100
0.2392
0.0885
0.777
0.878
0.2283
0.8143
0.9198
1.0477



X

0.1879
0.1484
0.1953
1.266
0.962
0.760
0.1878
1.0005
0.7902
1.0399






0.0286
0.0087

3.2S7
0.0106


2.6981
0.8208





0.1579
0.1363
1.158

0.1196
1.3202
	 	
1.1396






-------
                                                CANCER
                                                              Brain and Nervous system
d  Urban  and Rural  Counties

£23  Rural  Counties  (counties  having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
••THrlr" T-,Mn ne Avera8ed 1st order rate.
Averaged 2nd order rate
"RUU" M -- Avera8ed lst order rate-
RISK rauxo or , - , . .
Averaged 3rd order rate
•Tink" r-iil -f AveraSed 2nd order rate
Averaged 3rd order rate
Sum of weighted rates:
•Ttlnht-d ri-k" ratj- af Avera^ed lst order rate
wexgnueu LJ.SK. LOLL.*.O QL ^ , . *
Sum or weignted rates
"Up-tal r 1 r-Uk" rari- cf Avera?ed 2nd order rate-
weignceo nsjc racio ot „ • • . , . :
Sum of weighted rates
"tfpiehr^d risk" -atla of AveraSed 3rd order rate.
weixLiu&CL rxstc j.auio 01. ~ » ; ; .
Sum or weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
Than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
4.0784
4.3283
3.9547
0.942
1.031
1.094
4.0235
1.0136
1.0758
0.9829


2.6748
2.6248
2.6556
1.019
1.007
0.988
2.6536
1.0080
0.9891
1.0008


2.0118
0.9971
1.5742
2.018
1.278
0.633
1.5464
1.3010
0.6448
1.0180


4.9574
0.4713
1.0169
10.518
4.875
0.463
1.4481
3.4234
0.3255
0.7022



-------
                                               CANCER SITE:
                                                                     «land
~f  Urban and Rural Counties

2£3  Rural Counties (counties having urban
    centers with  100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ri-t" ratio of Avera§ed lst order rate-
.RISK jrauio O£ : r , . •
Averaged 2nd order rate
»-l-k" --jria af Avera8ed 1st order rate.
JxlStC raulO Or ~ : r : ; .
Averaged 3rd order rate
"•M^k" , r-iT -f Avera8ed 2nd order race-
KISK ratio oi . , _ , . :
Averaged 3rd order rate
Sum of weighted rates:
"U lal-r 1 rl -k" r^ri- -f Avera?ed lst order rate-
wexsnted nsic racxo oz r - : . .
6 • Sum of weighted rates
"'J I-li 1 risk." rarl- -f Avera§ed 2nd order rate
nexKutBu nstc racxo or ^ ^ t ; ^ •
5 Sum of weighted rates
"•J;'la'i 1 ri-k" rat-i- -f Avera8ed 3rd order ratG
tveigntea risn ratio or - , . . , . :
0 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Males Females Males Females
0.4758
0.5146
0.3514
0.925
1.330
1.440
0.3948
1.2052
1.3034
0.9053




0.7330
0.7144
0.7243
1.026
1.012
0.986
0.7240
1.0124
0.9867
1.0004




0.0365
0.0145
0.3741
2.517
0.098
0.039
0.2754
0.1325
0.0526
1.3584




0.1952
0.1366
0,3914
1.429
0.499
0.349
0.3296
0.5922
0.4144
1.1875





-------
                                               CANCER SITE:
                                                             Endocrine Organs
f~T Urban  and Rural  Counties

C*j Rural  Counties  (counties  having  urban
    centers  with   100,000 inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"TM-v" ratio of AveraSed 1st order rate.
.IV.LSK rarxo or ~ : - . ,
Averaged 2nd order rate
"lit a" rji i ; nf Averased lst order rate-
KISK ratio or ~ r . , .
Averaged 3rd order rate
"rii.L" run f Avera&ed 2nd order rate-
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:
HT, _, , ., i «t • * Averaged 1st order rate
6 Sum or weighted rates
"u tit 1 ritt" ratio cf Avera^ed 2nd order rate.
e Sum or weighted rates
"u i-ti l ,-1-L" i-Ari- of Avera8ed 3rd order rate
wexgntefl risK. ratio 01 _ •*, .i_^j ^
0 Sum or weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
0.2758
0.2757
0.2614
1.000
1.055
1.055
0.2653
1.0396
1.0392
0.9853
X

0.2759
0.1591
0.1941
1.734
1.421
0.820
0.1994
1.3836
0.7979
0.9734


0.0440
0.0272
0.0480
1.618
0.917
0.567
0.0444
0.9910
0.6126
1.0811


	
0.0471
0.0427
	
	 ._..
1.103
0.0378


1.2460
1.1296



-------
                                           CANCER SITE:
                                                        Bone
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ul ik" ratio of Avera§ed lst order rate-
Averaged 2nd order rate
"ti k" nti- -f Avera§ed lst order rate
XJ.;»K ratio or ~ ; - . . •
Averaged 3rd order rate
""• L" -jti- ^f Avera8ed 2nd order rate
iv3.SK. rauio OL T t ^ i . .
Averaged 3rd order rate
Sum of weighted rates:
"U luht-pd ri-k" ratio of Avera8ed lst order rate -
wcxstiuBu rxsic raui.o oz :; -. . \ . •
6 Sum of weighted rates
.... . , , . .„ ±. f Averaged 2nd order rate.
WGiKnt&u ris.c rac.LO or r .. - . ; , .
6 Sum of weighted rates
"u -ai i -j ri-k." rnri- af Averased 3rd order rate.
Weighted risk ratio of Sum Qf weighted rates •
Trends
The averaged first order rate is greater
than che second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than che second order rate which in turn
is greater than the .first order rate.

White White Non-white Non-white
Males Females Males Females
1.3431
1.4135
1.3269
0.950
1.012
1.065
1.3412
1.0014
1.0539
0.9893




0.8122
0.8450
0.8821
0.961
0.921
0.958
0.8682
0.9355
0.9733
1.0160


X

0.5750
6.9115
1.2838
0.083
0.448
5.384
2.0297
0.2833
3.4052
0.6325




0.1433
0.2245
0.9112
0.638
0.157
0.246
0.7140
0.2007
0.3144
1.2762





-------
                                           CANCER SITE:
                                                         Connective  Tissue
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)


Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Risk" ratio of-

"Risk" ratio of
Sum of weighted
"Weighted risk"
"Weighted risk"
"Weighted risk"
Trends
Averaged 1st order rate.
Averaged 2nd order rate'
Averaged 1st order rate
Averaged 3rd order rate"
Averaged 2nd order rate
Averaged 3rd order rate'
ratesT
- Averaged 1st order rate.
Sum of weighted rates
Averaged 2nd order rate
Sum of weighted rat.js
, Averaged 3rd order rate.
Sum of weighted rates

The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater Chan the first order rate.


White White Non-white Non-white
Males Females Hales Females
0.5190
0.6483
0.6191
0.800
0.838
i.047
0.6105
0.8501
1.0619
1.0141




0.3785
0.5478
0.4580
0.691
0.826
1.196
0.4608
0.8214
1.1880
0.9939




0.3568
0.7236
0.3638
0.493
0.981
1.989
0.4166
0.8564
1.7369
0.8732




0.3954
0.2246
0.6983
1.760
0.566
0.322
0.5915
0.8581
0.3797
1.1806





-------
                                                CANCER SITE:
                                                             Hodgkin's
!I  Urban  and  Rural Counties

\~X~i  Rural  Counties  (counties having urban
    centers  with 100,000 inhabitants are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
..r-.-k.. ran-, Of Averaged 1st order rate.
RISK racio 01 i , r , , .
Averaged 2nd order rate
"•ML" r^i-1- -f AveraSed 1st order rate.
Kistc ratio or — r^ . • , .
Averaged 3rd order rate
"ri-U1 -.111- -r Averased 2nd order rate
Risk ratio of Averaged 3rd order rate-
Sum of weighted rates:
"u fir \ -1-1." rarl- -f Avera8ed 1st order rate.
weigntea rxstc. ratio 01 _ - , .
e Sum of weighted rates
"u r-lr i -ll" i-ari- if Avera?ed 2nd order rate
Weighted risk ratio of Sum Qf weighted rat£S -
,,y . . . • ,» --tl- af Averaged 3rd order rate
Weighted risk ratio of gum Qf weighted rates •
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the .first order rate.

White White Non-white Non-white
Males Females Males Females
2.1772
2.1638
2.2358
1.006
0.984
0.968
2.2182
0.9815
0.9755
1.0079




1.1993
1.2136
1.3145
0.988
0.91-2
0.923
1.2860
0.9326
0.9437
1.0222


X

0.9282
3.9428
0.7054
0.235
1.316
5.589
1.2186
0.7617
3.2355
0.5789




0.4193
0.1964.
0.7189
2.135
0.583
0^273
0.6056
0.6924
0.3243
1.1871





-------
                                           CANCER SITE:  Lymphosarcoma
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Ri-k" ratio n£- Avera8ed 1st order rate.
K.XSK rauxo OL T^ : 7 r^ 	 , .
Averaged 2nd order race
"TM ,-!,•• T-zrin nf Avera2ed 1st order rate.
K.ISK ratio or j r — , , 	 •
Averaged 3rd order rate
"111 5k" rri- -' Avera8ed 2nd order rate.
XU.5K ratio Oi. — ~ r 	 	 ;
Averaged 3rd order rate
Sum of weighted rates:
"W-ial L J -i£t" riti- f Avera^ed 1st order rate
weignteo nsK ratxo or _ ... . , . .
Sum of weighted rates
"TJMal i J r-uk" rati tf Avera^ed 2nd order rate
Weighted risk ratio o£ gum of Wfcightad rates .
"U it) i 1 r--c.i" i-Ail- f AveraRed 3rd order rate
wezznteoi riLSK. ratio or ~ _ ; ; , .
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
.than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Males Females
4.8484
4.7359
4.6538
1.024
1.042
1.018
4.6902
1.0337
1.0097
0.9922

X

3.2486
3.1958
3.2166
1.016
1.010
0.994
3.2176
1.0096
0.9932
0.9997



12,5699
3.3140
1.2497
3.793
10.058
2.652
3.0673
4.0980
1.0804
0.4074

X

1.0770
1.3418
1.2338
0.803
0.873
1.088
1.2290
0.8763
1.0918
1.0039




-------
                                                CANCER SITE:
                                                             ICD's not listed
r~[  Urban and Rural Counties

\X]  Rural Counties  (counties having  urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Hi-lc" nrli of Avera«ed lst order rate-
Averaged 2nd order rate
"n-,-k» . . _- Averaged 1st order rate.
KILStC ITati O OT . 	 — , _ , v — *
Averaged 3rd order rate
««,.-,-.-•• V-M- -f Averaged 2nd order rate.
K.ISK ratio oi . . — - , ,
Averaged 3rd order rate
Sum of weighted rates:
"U icli d rick" rait- -f Avera«ed lst order rate-
6 Sum of weighted rates
"U -lul i 1 -i-k" rait f Averaged 2nd order rate.
wei&utecL rxstc ratxo oi ~ ^ ^ ; , .
Sum of weighted rates
"u iiit i i • i" -AH r Avera2ed 3rd order rate
Weighted risk ratio of Sum Qf weighted rates -
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.

White White Non-white Non-white
Hales Females Kales Females
9.6478
9.3495
9.5897
1.032
1.006
0.975
9.5629
1.0089
0.9777
1.0028



i
9.3665
9.0721
9.4003
1.032
1.036
0.965
9.3498
1.0018
0.9703
1.0054




0.7299
4.3872
13.7450
2.218
0.708
0.319
11.8121
0.8237
0.3714
1.1636




12.5807
7.3556
9.1978
1.710
1.368
0.800
9.3718
1.3424
0.7849
0.9814





-------
                                           CANCER SITE' ^^ Malignant Neoplasms
Urban and Rural Counties

Rural Counties (counties having urban
centers with  100,000 inhabitants are
excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"ri.nl-" rntia of Avera?ed lst order rate-
Averaged 2nd order rate
"rr-k" TAI t f Avera?ed lst ord£r rate.
KJLSK ratio or ~ , ,. . ,
Averaged 3rd order race
,,r, _,.., r,ri- -f Averted 2nd order rate.
Risk ratio of Aver^,ed 3rd order race-
Sum of weighted rates:
"U-iEht-J ri-k" rAtia of Avera8ed lst order rate
Sum of weighted rates
"U lahtiJ rl-k" -id- cf AveraSed 2nd order rate
wexKHLeu nsK tatiLO OL r ^ ~ r~-" , .
* Sum of weighted raues
"u luht^l risk" r^i-i- af AveraSed 3rd order rate
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
.than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Males Females Hales Females
165.7077
160.4561
152.8577
1.033
1.084
1.050





X

130.0746
127.3310
127.1617
1.022
1.023
1.001
127.5475
1.0198
0.9983
0.9970

X

140.1987
135.1143
134.2927
1.038
1.044
1.006
135.2029
1.0370
0.9993
0.9933



131.4023
148.4828
108.0597
0.885
1.216
1.374
116.8480
1.1246
1.2707
0.9248




-------
                                                CANCER SITE:
                                                            Leukemia
CJ  Urban and Rural Counties

["XT  Rural Counties  (counties having urban
    centers with  100,000  inhabitants  are
    excluded from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"iH-lr" ratia cf AveraSed lst order rate-
KJLSK ratio ot ~ . r ~ .
Averaged 2nd order rate
"Pt-l" r*rt- -f Avera§ed lst order rate-
KISK. ratio or . , « , , •
Averaged 3rd order rate
,,r. ,„ . f Averaged 2nd order rate.
Ki*k ratio of Averaged 3r, ^rder ratg.
Sum of weighted rates:
"•J r -i-i 1 rl-L" rati- of Avera^e
-------
                                                CANCER SITE:  ***iplB
    Drban  and  Rural  Counties
1*7  Rural Counties  (counties  having  urban
    centers with  100,000 inhabitants are
    excluded- from analysis.)
Averaged 1st order rate:
Averaged 2nd order rate:
Averaged 3rd order rate:
"Hint" rntia af AveraBed 1st order rate
Averaged 2nd order rate
"-IQL" rarl if Averased 1st order rate.
Averaged 3rd order rate
"IHt,-L" rat! -f Avera8ed 2nd order rate
Averaged 3rd order rate
Sum of weighted rates:
"U -isl tad r^L" r-itia -f Averased 1st order rate.
wei.2tii.eci rxsjc ratio ot ~ ^ ^ : , :
6 Sum of weighted rates
"u r it J L-J L" i -f Avera«ed 2nd order rate-
wexsnccu rxsic rauio ot r ^ ^ : ; •
s Sum of weighted rates
"u--mt-J ri-i" r^rl- 3f Avera&ed 3rd order rate.
weiguceu risK. ratio or ••- , .
6 Sum of weighted rates
Trends
The averaged first order rate is greater
than the second order rate which in turn
is greater than the third order rate.
The averaged third order rate is greater
than the second order rate which in turn
is greater than the first order rate.
White White Non-white Non-white
Kales Females Hales Females
1.6703
1.7654
1.7115
0.946
0.976
1.031
1.7139
0.9746
1.0300
0.9986


1.2923
1.1488
1.1624
1.125
:1.112
0.988
1.1766
1.0983
0.9764
0.9879


1.0636
1.7415
1.4088
0.611
0.755
1.236
1.4124
0.9402
1.2330
0.9974


1.8625
0.5793
1.9602
3.217
0.950
0.296
1.7504
1.0640
0.3310
1.1198



-------
                   APPENDIX II
    Study Questionnaires and Survey Instruments
for the Study of Great Lakes Commercial Fishermen
     - Telephone interview questionnaire (pilot)  Protocols 1 and 2
     - mailed questionnaire (pilot) Protocol 3
     - telephone proxy questionnaire (Pilot)
     - Great Lakes Fishermen Health Survey - Large Cohort

-------
                                                 Telephone Interview (Protocols  1 and 2),

                                                            KIMIl:icHKH IIKAl.TIt SIIIIVKY

                                           INSTRUCTIONS I   Flenoc enoucr (lie fallowing qucsttong ant
                                           return the questionnaire within five days.  Uao the enclosed
                                           Kt raped, self-addressed envelope for Bailing.  Tour anewcm
                                           are confidential and will only be used for research purposes.
                                           Your identity will not be associated with the survey results.

                                           If you cannot give un exact answer, provide your best estimate.
I.  Whac ie your currcue ccMreisef
                                  (street)
                                  (city)                            (state)            (tip)
2.
1.
4.

5.
«.
•Whet
Utot
What

Uhat
What
la
is
U

ie
lit
your
Dent
your recel
your

your
your
,
1
O-le
)£) White
birthdeteT

Social
narital

nonth
2)C
| Fencle
2)Q Black :
1
day
»


year
!)("") Hispanic *)Q American Indian 5)^") Other




Security number? - - .
status? l)(
"^Slnitle

.arrleJ) 2)C*} Harried 3)f^ Separated
or divorced *)(~} Widowed
7.  Do you presently hold a commercial fishing license?    ))(  )Ho     2)( ) Yes—"-Approximately over wliot years liave you held c license? 	/	
                           	^~/          ^                                                               T7o*~"To~~
                           1                 * ~ °  fc"~"'                                                                                 (go to question 10)

6.  Did you hold a coK*erclal (lilting license in the past?  I)(y Ho     2)^) Yes—»- Approxlnately over wlutt years did you hold a license? 	/
                                                                                                                                            froto   to
                                                                                                                                      (go to quest loci 10)

-------
9,  Btv« you ever been a crew nesber for, or e partner wtth^sn Individual owning K coaaerciel ftehlng license?

       t)(_) Ho     2^O *ee-—»  Over vhet years have you been e crew oenber or partner      /
           I                                                                               from   to      (go to question; tO)

    (If Ho, plena* ctop here end return the questionnaire »6 coon ae pocelble In the addreeeed envelope.   Thank you for your  cooperation.)
0.  Hear what town* do (did) you commercially fish Boat  often?
0*. Where It (was) your hooe port located!


    City'State

-------
I.  16 cemerclel (Ttcblce year cttinreae  t)(~)Ko
    full-tine oecstpetioaf
                                                                     12.  Wluit  in your curr  '—#0»«r vhflt ye«rs tutve  you been e pert-tine ficbenua?	/	
                                                                                                                                            fcos   to
                                                                                                                                     (go to queetloa 14)

                                                                          2 )f")lfee —(.Over ubat years wera you full tteof      /
                                                                     	from   to
                                                       Ower whist  JCECR  heve you cotmcrc Jelly flched full tUset
                                                                                                              __
                                                                                                              froo   Co
                                                                                                        (go  to question  I
ft.  Da you currently 
-------
   Tu  Hi"' In-ill  ul  yiiif l'iMiw|i'iinl mitt |'ivnm()
                                                                                                                                        Employed
                                             Street
                                                                             City
                                                                                                         State
                                                                                                                                       •fro«
                                                                                                                                                  To
..  Do  (or  did)  you confute any of the flah you catch commercially or o» a (port fisbernan?  I ) (~) Yee   ;  1 )
I.  Approximately how eany of your neala contain fish caught  (by yourself or a friend) from the Great Latest
         1)  _ per week  (or)  2) _ per month
                                                                                                                   No  (If No, go to queetioq 28)
I.  What  types of Creat lakes flab do you eat Boat oftent
   	Saloon          Walleye           Hough flah
                                                                                       tako Trout          BatB
   	Perch	Burbot     	Chub     	Lake Whitefiali          Smelt          Lake Hotting     _
   ApproxJaately how pany year* have you conauaed Great Lekee  fleh with thle frequency?      yetre
    	Northern;Pike     	
Lake Whitetieli          Saelt
	Paa  flah
Otheri  (epeclfy)_
                                                                                      Other Trout
!.   Approximately how nany of your ceale contain fish  ceughc  (by youreelf or & friend) fron watere other  than  the Great
         1) 	per week  (or)  2)   	per sontb

-------
     Whet types off B»tn®«; intend «3tecc" Cieb do you «»t >o»t of Cent
     _ ___ Seleoa     _________ Kellerc          Hough fiuh     _ Northern Pika
          Perch
                         Burbot
                                         Chub
                                                       Lake Uhiteflch
                         _ take Trout           Bane
                       Smelt     _ Luke Herring
                                                                                                                   __ J?aa flub
                                                                                                                     Other I  (/specify)
                                                                                                                                           Other Trout
24.  Approximately tow cusny ye* re heve you conduced inland w«tes fish with  thte  frequency!
                                                                                                    yeere
25.  In conpr.ric.on to your col f>  Bow often doer, your wife eat lleM  How of tea do your none c./st fieht  Botr often do  your deughtere  one Cleht
      tflfel                                             Sons i                                               Daughter*!
      i) (___) Ac often f-e ajreelf                          l)(
      2)    (tore often then ayeelf
      3)QLe6c often then caycclf
      *)Q Does not eppty
      5)    Hever
l)(Jie often e« myeell
2) Q More often th«n oysolf
1) Q Leee often than nyr.clf
*) Q Doec not apply
5)    Never
                                                                                                             1)     As often  «e nyfiotf
                                                                                                             2) Q More  often than cyeelf
                                                                                                             3) Q LCCE  of tea than
                                                                                                             4) Q Doee  not  apply
                                                                                                             5)     Never
26.  Approximately how eany pounds of ft eh hcvo you consumed pec yeert
            (r.asuBd  1 f(eh peel equate *| pound of filth)         pounde

11.  Of the fleh you c«tcb end consume, whet percentage of the fish ere prepared by eech of (be following eethodeT
      1)  Broiling       1
      2)  Pen frying 	X
      3)  Siwked 	X
      4)  Boiling
      5)  Patched
      6)  Other

-------
28.   Do you Drcxatly BBok* cigar«tta«T    1)0"°    2) O ***
                                                                         29,     How old uere you when you Eiret began to evoke eigeretteef
                                                         30.    What la th* «vet«g* otuiber of cigarette* you presently BooKe per d«yT
                                                                        31.    Bow many y««r* b«v« you naked el««r«tt«s wsth title Itcqueacjl  	
                                                                                                                                    (go to queetioa 34)
2S«.  DM you «ofee elgcretcce in  the paetT    l)QKo    2>OT

                                             (go to question 34)
                                                                                        32.   Bow old were you when you fleet bcgefi to Esott
                                                                                        33.   BOB Beny years din! you csofcc
                                                                                                                             ($o to quoctloa 34)

-------
24.  Do jva presently moke e nlpef  I) Q Ha     2)Qtec
                   Old you moke & pipe tn the peetj
                             (If to. go to queBtiOa nufibcr 40)
                                                                         35.  HOB old uere you whca you firet begeo to tssokc e plpef_
36.  Ohet £c the evercge auzsber of plpafule you pceccntly make pec 
-------
«0.  Do you preoeocly eawke cigar*?  1)0"°     2)
                                                                     41.  llotr old were you when you began to moke cigar*?
42.  What IB the average [timber of clg*c> you preeently onoke per day?
                                                                     43.  Bow nany year* have you aaokeJ cigar* with thio frequency?
        J>0e.  Did yo«i moke clger* In the peat  t)Qlto     2)Qtec


                        (If No, go to queetlon number 46}
                                                                                                                           (go to question 46)
                                                                                         44.  Botf old were you when you begea to eaoke
                                                                                              clgarel ______
                    45.  Row eany yearn did you esoke
                                                                                                                         (go to questloa 46)
46.  Have you ever chewed tobacco regularly?  l)) No     2)    tea

47.  Have you ever ueed anuff regularly?  l)Qlto     2)QTcr,

48.  Have you ever smoked non-tobacco products regularly?  l)No

-------
                                 4Urd liquor it coaelder«d  to  Includei  Clo| UhUkcy{ 8coceh( Vodtej Eun( Brendy
                                  (A drink - I  to 11}  OK. of elcohol.)
$.  Do you detak Etecd ilquoref   J)     Ho
             Did you eoaeum« hard  llre la   the  peett
                                                                       SO.  Jlow old vere you vheo you fleet bognn to dclnk herd llouoref
SI.  .Uhct le the svetege mrabcr of bard  liquor drlnfec  you eooetme per week?
                                                                       52.   Dou cany yeeie have you coaeuued hard liquor ulth Cblc frequeacyt
                 Sli   How old ware  you when you flrct begem to drink herd
                      llquorf  ____.

                 S4.   Uou ncny year* did  you COOOUKO herd liquor)  	
                                                    (go to queue loo SS)
                                                                                                                              (go to queatioa 55)

-------
55. Do you drink becrT  D£)HO     2) £) fee -
      55c.  Did you drink beer la the p«ctT
                                                   v
                                                                      56.  Hoy old  were you  when  you f tret  b«ge» to drlafc beer?
57.  What 1« the everege number of beeta (12  ot.  bottlen)  you preeently coasuse

     pet week?   ________
                                                                      58.   How many ye«r* have you consumed beer with thie frequeocyT    .



                                                                          :(go to quoBttoo 61)
                  59.  Bov old were you uhea  you first  began to drink




                  60.  Ho« ectty ye*re did you coamnse beort  _^__
                                                   v
                                            (go to question 61)
                                                                                                                          (go to queetloa 61)

-------
Cl.  Do 709 drtob efesetf  S)Qt«»     ?)
       6lE.  DW 700 drink wine in the peett t)Qlto   2)QY


                                                   4*
                                           (go to o.ue*tlon 67)
                                                                        62.  Sov old tfere you eban you flret began Co 
-------
ilave you, your «Het or any of four  children ever had sny of this following diseases or conditioner  (Flense check appropriate  boK.)
67.

68.
69,
70.
71.

72.
7J.

74.

75.
76.

77.
78.
79.

80.
81.


82.
61.
A«tte« t)(jHo 2)QjTfee
y~>k /~*v
Bronchltle l)f~}Ho 2)(~JYea
Emphysema 1 ) Q Ho 2) O YeE
Tuberculosis- TB I)f~)Ho 2)r~)7oB
HononucleoEie-Moao- ^_^ ^_^
Kissing Dlaecee l>OHo 2) (J) Yec
Pneuftonifi OT ) No ^) C ) ^e®
Any other diseaee of
the respiratory
Eyaten
Specifyi l)^~^Ho 2) (^J f 06
Hepatitis or yellou ^^^ ._
jaundice l)f")Ho 2)(jYee
S~\ S~*L
Clrrhoalv Of") Bo 2)r"jYee
Any other liver dleesse _.
Specify: l)()No 2)()Ye6
Spondylitie l)QHo 2)OTee
Cout l)Olto 2>OVee
Rhcunntold Arthritis l)(~)Ho 2)(~)Yee
>^^ ^*N
Osteoarthrltla l)(~JHo 2)^J tee
Any other dlseaeen of
bonea and joints
Specifyi DliMo 2)<^)Tea
High triglyceridea l)(^) Ho 2)(~)lTe«
Menlngltla l)(~)No 2)OIee
8A.


85.
86.
87.
88..

89.
90.
91.
92.

93.

94.
93.
96.


97.
96.

99.

Hypertension or high
blood presoure t)f}Ko
^-^
High cholesterol l)^JNo
Angina pectorle l)(~)'lo
Heart attacli-KI or coronary 1) (_)NO
Stroke, cerebral accident.
(CVA)-heoorrhage( throaboelB,
efabolisia l)C ) "°
Any other heart or circula-
tory dlseaoa Specify) l)^~)Ko
Diabetes 1) [t Wo
Thyroid it Is l)(~}Na
Any other glandular disorder ^^^
Specify! I)l j Ho
Eye dleeasee
Specifyi I) O«o
Paoriasie I)Q~JHo
Eczema I)^JHo
llerpea totter-chlnclcc ^^^
(dennatitla) l)(_J)Ho

Penphigue l)C^Ho
Any other dlaeaaea of ekia ,^.
Specifyi . I) 1 j Ko
Multiple Sclerocia l)(~)Ho


2) C)
^^
»o
2>O
2)Q

2>O
»o
2)C~)


2)(~)

2>O

«o



2)O

2)f*^)
2,O

                                                                                                                             Tee

-------
tOO.  Any other diseases of  the

101.
102.
103.
104.


105.
106.
107.
108.
109,

110.
111.

112.

113.

114.
115.
116.
Specify:
Anefflia
Gastritis
Dicers (stomach or. duodenal)
Any other diseases of the
digestive system
Specify: _
Cancer of the breast
Cancer of the stomach
Cancer of the esophagus
Cancer of the mouth/tongue
Cancer of the large
intestine
Cancer of the rectun
Cancer of the trachea,
bronchus or lung
Cancer of the liver or
biliary passage
Cancer of the bladder
or urinary organs
Cancer of the skin
Cancer of the thyroid
Leukemia
l)0Mo
*«-^^
1)0 Ho
l>O«o
1)0 Ho


1)0 No
DO*
1)0 No
1)0 No
1)0 NO

D0No
DO"0

1)0 No

1)0 No

l)0Ho
1)0 Ho
1) 0No
1)0 No
2)0 Yes
2)0 Yes
2)0 Yea
2)0 Yes


Yes

2)0 Yes
2)0 Yes
2)0Ves

2)O Ve8
2)OYe*

2)0 Yes

2>0Yes

2)0 Yeo
2)0 Yes
2)0 Yes
2) O'Ves
117,  Other cancer
      Specify:	
                                                                       118.  Mental retardation
                                    1)0 No     2)0Ye«

-------
    For ea4h of ttie diseases you  have Ben cloned  la your I Rail? Ket.:bcSv. ploo.-.i nail not
Name of disease/
condition
Homes of family members who have the
disease/condition:




Names and addresses of the clinics, hospitals, or
doctors consulted:




Date of diagnosis:




Name of disease/
condition
Names of family members who have the
disease/condition:.




Names and addresses of the clinics, hospitals, or
doctors consulted;




Date of diagnosis:





-------
    For (Mich of the diseases you have mentioned In your faatly xeabare,  please Call net
Name of disease/
condition
Naaes of family meabers who have the
disease/condition:




Names and addresses of the clinics, hospitals, or
doctors consulted:




bate of diagnosis:




Hame of disease/
condition
Kames of faatly members who have the
disease/condition:.




Names and addresses of the clinics, hospitals, or
doctors consulted:




Date of diagnosis:





-------
             of tltc diseases you have ocncioncd in your family Eeatjers, ploaoe  tell oe»
     of disease/
condition
Names of family members uho have Che
disease/condition:




Names and addresses of the clinlco, hospitals, or
doctors consulted:




Date of diagnosis:




Name of disease/
condition
Names of family members uho have the
disease/condition:




Names and addresses of the clinics, hospitals, or
doctors consulted t




Date at diagnosis:





-------
    For each of tlws diseases  you tutve mentioned In your foali; rstmbere,  pluaeo tall net
Kane of diaeisee/
condition
Names of family members who have the
disease/condition!




Kaaee end eddressee of the cllnlce, hospitals, or
docCori consulted)




bate of diagnosis:




Name of disease/
condition
(lames of faally meabera who have the
d tsease/cond It Ion : .




Names and eddrecses of the cllnlce, hospitals, or
doctors consulted:




Date of diagnosis:





-------
    tor'fcacli of the diseases you have acnttoned in your  Cosily senberu,  please  tell met
Name of disease/
Condit ion
Names of faintly mrmbcre who have the
d tscase/cond It ion :




Names and addresses of the clinics, hospitals, or
doctors consulted:




Date of diagnosis!




Name of disease/
condition
Names of family members vho have the
disease/condition:.




Names and addresses of the clinics, hospitals, or
doctors consulted:




Date of diagnosio:





-------
H.IVC fixi tivni li*i( any {>f tlm lot liiwlug
cyeptoce?

119. Severe fever
120. Extreme tiredness
121. Frequent or very bad headaches
122. Problems with uouth or throat
123. Any problems with ears or hearing
Specify:
124. Any unusual difficulty with eyes
or eyesight other than a change
of the prescription of glasses?
Specify:

125. Sudden weakness or heaviness of
arms or legs?
Specify:
126. Numbness in arms or legs?
127. Swelling in arms or legs?
128. Stiffness in joints or bones?
Specify:
129. Pains in joints or bones?
Specify:
130. Spasms of limbs?
131. Spells' of dizziness?
132. Have you fainted or blacked out?
133. Very strong heartbeats?
134. Irregular (fast, slow or
inconstant) heartbeats?

Ho




	




	

Tee









... .


	

II. iw ti I'ljiirnl ly
Uoctf (or did)
thle occurf
(deye, tion.the,
or year*)

















II. iu IIIIIK tin
eynptone leecT
(days, aonthe,
or yc/srs)


















Did you ccncmlt
a doctor!
No Yea




	




























Doctor's nr,r,e & address

















Year oast
recently
experienced

















-------
Have you ever li«ii Buy of tlie fulluui
symptoms?
135. Have you hail pain, discomfort,
or trouble In or around your
licnrt ?
136. Itching of the skin?
137. Any unusual discoloration or
eruptions on the skin?
Specify:
138. Any problems with your stoooch
or digestive system?
Specify:
139. Swollen glands in your neck,
armpits or groin?
140. Have you lost 20 or more pounds
in the last five years?
(include dieting)
>8
No






Yes






How frequently
does (or did)
this occurT
(days, nontha,
or years)





X
{low long to
(or did) the
oymptoffls laat't
(iScys,, raontiiBj
or years)





X

Did yoi
a doctc
No






i consult
>r?
Yee






Doctor's naae 6 address






Year most
recently
experienced







-------
'HIE U3T SECTION OF TllE QUESTIONNAIRE RELATES  TO YOUR WIFE AND CHILDREN.  IF I HAY SPEAK TO YOUB WIFE, SUE MAY BE ABLE TO ANSWER THE FEW QUESTIONS
REMAINING.
1.  How nctny netursl children do you have?

    How ttuny eicp or adopted children do you hove?

    Plcaee tell »c of ell pregnancies onJ outcomes of which you were the father/Bother in the sequence they occureJ.
Prcg.
t
1.
2.
3.
4.
5.
6.
Complication® in
till a pri/giumcy
(describe)






fhif como
Mlncacrlngo






Stillbirth






Live Birth






Other






Infl
ai
M






mts
iK
F






Data of Birth






Live
Birch
Weight






Hospital, clinic or
place of birth






Doctor 'a name






  .  H«ve any of your natural children died?  (do not include Btillblrtha nor step or adopted children)
        I)    No     2)    Yes  (If Yes, please complete the following teble.)
I.
2.
3.
4.
Sex
M f








Data of Birth




Date of Death




Cause of Death




City end Stete in which death WEE reported





-------
142.  Do you knou of  any mental or physical abnormalities at birth (birth defects) in yourself or your natural children.


             1)0 No      2)0 Yes    (if yes, please complete the following table.)


                i
     (go to question  143)
1.
2.
3.
4.
(clat ionsMp to you
>clf Son Daughtei












[Check appropriate boxes)
Mving




Dead




Nature of Birth Defect (describe)





-------
141.  Ba you O*«  <" te«i «n««er qaeetlooe 144 end US)
                                  (£o to  question 146)
144.  Khist DCtNod of birth control hive you used the  Bate  during your  edult  llfeT
       uQpltl     2)QlUD     J)QdUphrn»     4)Qfo«e or Jelly     S)£) rhyths     6)Qcoado-   7)Qother (specify)r
ItS.  *t what c0c did you begin ueing birth control  rsethodel ________________
1*4.  M whit Bge 414 you begta to neaetruite}              	
Utt, Etvc you (topped Reattruitlng?   l)QJ)Ho                 J)^J)YeC
                               (1C  Ho, go to qucotloa  149)
UT.  At «h»t c0e did you stop MmttruttUfct  	
141.  DU the ceototloa occur naturally or due to eurgeryt
                           2) (_) Surgery  	^ Uhct u«c the rcx«oa for the eureeritt                             ,
                                                 Doctor'^ ofi&e  *nd addreecT	^^
                                                                          DCBC                             tddreee

149.  Uh*t le (me) the ever BE e  length of your nenetrucl cycle!       dayg (Froa flret dey of bleeding Co fir it diy of next  period)

ISO.  da the everege,  how long  1>  (w*«)  your period!       d«yt

lil.  In the p«et five yecr«( tut  your blood (lou during oenatruetioa  !)(_) Increaaedt     2)^J) dccrcoecdt     3) (~) atayed  the eiuieT

1S2.  Biv« (or did) you experience eny tbnortxl  spotting or bleedln| between yout eenatrual cycleaT  l)("jNo     2)fjTcc  (go to queatlon
                                                                                          (go to ouectloa 154)

liJ.  It yee, «ld you  concult a  phyclclanT  ' l)(~) No     2)(")Yea     Doctor'* naae t adJreee
                                              ^-"^          ^^                                 K*M«
                                                                                                                         eddreec
t$4.  Have you__ucc<*,  or dld^ou  use,  any taedicationc prescribed by * doctor for ccnetrual Irregulirltiesl
                               !B	 » Mama of medication                               	
Queetlone 1-140 have been anawered by
Queatlone ttl-tS) have been anawered.by
Thank you for your cooperation In th|a health  eurvey.  Pleaae algn the conacnt fora and return the queationnaire In the eAcloaed  self-^eddreaeed  atanpcd
envelope.

-------
                                                    Mailed  Questionnaire (Protocol 3)

                                                             HKIIKMHKN  IIKAI.TII MIKVKY

                                           INSTRUCTIONS!   Please annucc  the following questions and
                                           return  tlie questionnaire  ultliln  five  days,  Use the enclosed
                                           stamped, self-addressed envelope for  mailing.  Your answers
                                           are confidential  end will only be uoed for research purposes.
                                           Your Identity will not be associated  with tho survey results.

                                           If  you  cannot give an exact answer, provide your beat collaiat
 I.   What IB your current  address?
                                   (street)
                                   (city)                             (state)             dip)
. 1,  What 1* yout «CK?     l)Hale     2)     Fcraale

 3.  What Is your racet     J)^~~N White    2)(~") Black    3)f~) Ilinpanic     4)^) Aaerlcen Indian     5)^) Other

 4.  What IB your birilxIsteT  _ / _ / _
                                nonth      day     year

 5.  What 1« your Social Security number? _ - _ - ______J____

 6.  What Is your n.trltal status?    1)T JSlngto (never  married)     2)( J llnrrled     3)f) Eepuratcd or divorced     *)( ) Uldowed

 7.  Du you presently liold a connerclsl fishing license?     l>r^)no     2)f J) tee— »• Appro* Irenlely over wl»t  yearo have you held e license?      /
                                                               •           ^"^                                                               (ron   to
                            r                •                                                                                         (go to question 10)

 8.  nid you hold « comerelal ftcliing license In the paetl   DV^^yMo      2)f) Yea— *- Approximately over v\t»t yoara did you hold ft HccnoeT _ _ /
                                                                                                                                             frotn
                                                                                                                                                    to
                                                                                                                                       (go  to  question  10)

-------
 t.  Kcve. you ever beea c crew uember for,  PC e partner ulth,«n individual owing e cotwercliit finding licence?
      t)lfc     2}Qtee—1> Over tthet  ;o«re.heve you been fi crew neaber or partner!      /        (go tp quencion iO)
         i
                                                                                         (roe   to
         *
    (If Ho,  pleeee stop here end return the queetlponeire  en  eoon re  pooelble in the eddreeced envelope.   Thank you  for your cocpcrctloo.)


(0.  Indicate (rtth ea "K" OB the Cficolced Ecp where you corotctclcUy  Clahed cost often.      (Tou ccy indicate core ttusn one locctloo).
It.  le coBiserctel fCehtag your current
               pccupetlooT
                                                                  12. Wat ie your current full-tiae pccupitlonl
                                                                  13. Wea coonerciel fishing ever your full tteo occupation?
                                                                      1 ) C_) Ho  ——pOver whet year* heve you been e pert-ttee
                                                                                                                                          fro*   tp
                                                                                                                                   (go to question 14)
                                                                      2 )^~)Yee —t- Over uhat yea re were you full tbtef       /
                                                                     	                                            {taf   ta (go to question 14)
                                                      • Over what years have you eoanercially flehed full tine? 	
                                                                                                              front   to
                                                                                                     (go to question 14)
14.  Do you currently owa c eport flehing llccnceT  i)^~^Ho  (go to question IS)      2)(~}YcB  (go to question  16)

IS.  Htve you ever oteatd e eport flehine licencet   l)QMo  (go to question 17)      2)QYe£  (go to question  16)

16.  Indicate with «a "0" on the enclosed cep where you eport fl»h(ed) noat  often.   (You aay  indicate more  than one location.)

-------
17.  To the  best of your fcnouloJge, ptcauc Hot llic nanca and adiircutica of full or putt-time crew mcraiiero?  (|iaHt iinJ (iruKu
Kane
                                              Street
                                                                             City
                                                                                                        State
                                                                                                                          Zip
                                                                                                                                     Employed
                                                                                                                                     From
                                                                                                                                              To
18.  Do (or did) you consume any of the fifth you cetch commercially or se e sport fisherman?  • )(_) YeB     2^
19.  Approximately hov stany of your actlt contain fish caught (by yourself or « friend) from the Great  l-afcesT
       1)      per week  (or)   2)      pec month
20.  How many of those nealo Include the following types of f ieh:
     _ Salmon     _ Walleye     _ Rough fish     _ Hot them Pike     _ Lake Trout     _ Base
     _ Perch     _ Burbot     _ Chub     _ Lake Vhltefinh     _ Smelt     _ iJike Herring'
 21.  Approximately hou aany  years have you consuned Greet  Lakes fish with this frequency?
                                                                                                  years
                                                                                                                  M°
                                                                                                                          ""' C° tO 1uascton
                                                                                                                   _ Pan fl«h
                                                                                                                    Other I (epectfy)
22.  Approxlaately  hou nany  of  your deals  contain  fish  caught  (by yourself or a  friend)  froa waters other than the Great Lttkeet
         1) _ per week   (or)    2) _ per nonth
23.  How nany of those deals contain the following types of fiah?
     _ Saloon    _ Walleye          Rough Klsh    _ Northern Pike    _ Lake Trout    _ Bess   _ Pan ri«h
           Other Trout
                                Perch
                                               Burbot
                                                               Chub
                                                                             Lake Uliiteflsh
                                                                                                     finelt
                                                                                                                   Lake Herring
                                                                                                                                          Other Trout
                                                                                                                                          Othert
                                                                                                                                                 specify

-------
2*r  tpproEtcxtcty host seny yccrc here you contused inland uet$r flch with  thle  frequency?          years
25.  How often da your foully tn«ber« eet £l«hf
      tttfei                                             Sonei                                                Daughters?
      t) ^J Ae of te& ec myeelf                          1) (_) JLe often e.e eyeelf                             I) (~y A
      2)Qhore often then eyeelf                      2) (_) More often then ayeelf                         2) (_) Hore  often than eyeelC
      3) Q Lece often then tsyeell                      3)(^)L«BE often thea ayeelf                         3^O L*ee  o£Ccn th*'n ^yeelf
      J&)("J)Docc not cpply                              4)(~JOoe8 not cpply                                 4)^~^Doee  oot ipply
      5)Q Hevet                                       5) Q Hever                                          5) Q «ever

16,  Approiclractely bow rjiny pound e of ft eh have you coneuaed  per yeec?
          (eeEuset  I fleb rstcl ccjuclc % pound of fteh)         pounds

27.  Uhct perceatsgee of flch you cetch end consume ere prepared by the following stcthodei
      1) Broiling      X
      2) P«n frylne 	X
      3> S«K>feed 	t
      «) Boiling 	X
      5) Poached 	X
      6) Other      I

-------
28.   Do you procatly (oak* cig«rett«>t    I)Q«o     2) Q T««
28».  Did you cnok4i
                                              in  the  peotf
                                                                        29.    Uou old were you whea you first began to coofce cig*i*ttacT
                                                         30.    What  le the nvercge nuaber of clgerettca you presently eaoke per
                                                                        31.    How n«ny jrcere Mve you taoked elgcrettec wnth thle fcequencyT  	
                                                                                                                                    (go to questIon 3*)
                                                            (go  to  qucotloo 34)
                                                                                        32.   Bow old were you when you flicc begcn to evofc* cig«rettA*T
33.   BOM anny yeerc did you eroke eigcrettetf  	
                                     (go to Question  34}

-------
34.  Do you presently snoka a pipe?  l)Qlto     2)
                                                                          ).  How old were-you when you first began to SB oka a pipe?
36.  What IR tha average number of pipefuls you presently smoke per day?_
                                                                         37.  How taany years have you smoked & pipe with this frequency? 	

                                                                        	                                               (go to question 40)
             34a.  Did you smoke a pipe In the past?  l)(~)No     2) (^) Yee


                                                         i
                             (If Ho, go to question number 40)
                                                                                       38.   How old were you when you first began to smoke

                                                                                            e pipe? 	
              39.  How many years did you smoke a pipe?
                                                                                                                          (go to question 40)

-------
40.  Do you presently smoke cigars?  l)(~)No     2)
        .40a.  Did you smoke cigars In the past  1) ( ) Ho


                        (If No, go to question number 46)
                                                                     4.1.  How old were you when you began to smoke cigars?
                                                                     42.   What  is the average number of cigars you presently smoke per day?
                                                                     43,  How many years have you smoked cigars with thta frequency?
                                                                                                                           (go to question 46)
                                                                                         .44.  How old were you when you began to smoke
                                                                                              cigars? 	
                                                                                         AS.  How many years 414 you smoke cigars?
                                                                                                                         (go to question 46)
46.  Have you ever chewed tobacco regularly?  !)(_) No     2) Q~J Yes

47.  Have you ever used snuff regularly?  l)^^No     2) (J Yes

.48.  Have you ever smoked non-tobacco products regularly?  1) f~*\ Ho
                                                                              Yes

-------
                                   ti-juor  tc cooeldered Co includei  Cinj Wbt«fce]r[ Scotch; Vodfce| Bust  Brendjr
                              (A dciak •» 1  co l
-------
55. Do yon drink bccrt  l)Qno     2) Q Ie« .
                                                                     56.  Hou old vere you when you fleet began to drink heart
                          57.  Uhat Is the average mmber of beers (12 or, bottle*) you presently consume
                              per veekl   ________
                                                                     58.  no« nany years have you coneuned beer with tht» freipiencyt    _

                                                                                                                        (go to question 61)
      55*.  Did you drink beer to th^ pcctt   l)QHo    2) Q Tec

                                                to qu
(|o to queatlon 61)
                                           59.  ROM old were you when you flret begta to drink beerI

                                           60.  Hou cumy ye«ru did you concuae beerT  ______

                                                                              (go to quectloo 61)

-------
it.  Da yoo drtofe tfteef
                               2)Qf«e-
6le.  Bid you drink vine in the peett  l)Qlto   2)Qt


                                           4*
                                    (go  to  question  67)
61,  How old were yea whea you fltet begea to drink wtncf       _






61.  Whet le the Evcc«$e nusbcr of wine drinks you bsve pec ecefef  ______






64.  lion tsony J«CCE hcve you coasuaed nine with this frequencyT	


     ~~                                             (go to quecstloa  67)
                                                                                  65.  How old were you when you fleet begun to drink wlne'T
                                                                                         66.  How o*oy ye*r« did you eooeuee


                                                                                                                             (go to queBttoo 67)

-------
Have you, your vif«, or ex>y of your children ovor had  any of  the following  diseases or  condition*.   (Fleece check appropriate box.)
67.
68.

69.
70.
71.

72.
73.

74.

75.
76.

,i.
78.
79.
80.
ei.


82.
8).
Asttsui 1)( JHo 2)1 )Yes
V — / '. v_y
Broachltis I)(_)Ho 2)C_)lem
_^
Eaphysea* l)(~)Ho 2)(J)Vc«
Tuberculosis- TB l)f~)Ho 2)(~)te«
Manonucleosis-Mono*
Kissing Disease l)f~)Mo 2)r~)Yes
Pneumonia l)f~)Ho 2)Q~^Vee
Any other disease of
the respiratory
ay 01 en
Speclfyt Of^No 2) ("^ Yea
Hepatitis ot yellow ^^^ ^^^
jaundice I) f") No 2)f}Yee
Cirrhosis "(2)"° 2* O *"
Any other liver diseaae
Specify: l)(_)Ho 2)lJYes
Spondylitls uQ"0 2>Olfee
Cout "O"0 2)OV"
Rheunatoid Arthritis 1)(2) No 2>OTe*
Osteoarthrltls I)("jHo 2)f"j Yes
Any other disease* of
bones and joints
Specify: I)i)Ho 2)^J) Yes
High trlglycerides l)(_~) Ho 2)/*jYes
Meningitis l)Ono 2)(^)Yes
8A.

85.

86.
B7.
88.

89.
90.
91.

92.
93.

94.
93.
96.

97.
98.

99.

Hypertension or high
blood pressure l)f~^No
High cholesterol l)fjNo

Angina pectorin l)(~^)No
Heart attach-Hi or coronary 1) C_JHo
Stroke, ctrcbral accident.
(CVA) -hemorrhage, throobosls,
eabolioa ')C3 "°
Any other heart or circule- ^^^
tory disease Specif yl O("jNo
Diabetes 1) I i Ho
Thyroidltle l)^)No
^~^
Any other glandular disorder _.
Specify, l)fl Mo
Eye diseases
Specify; UtlNo
Psoriasis O^~)HO
Ectena l)ONo
Herpes zoster-shingles ^_
(dermatitis) l)(~^Ho
Pemphigus I)(~)NO
Any other diseases of skin _.
Specify: DlMMo
ffciltlple Sclerosis 1)ON°


2)OTee
2) (~) Yes
^^
2)O™
2)OY"

2)Q Yes
2)0*"
... /~\ y
2)O*««
\—/
2>O1t««
\~s
2)Ote«
' V-/
2)0*"
2)OTe«

2>O »••
2)QY"

2)^~) Ye«
«O'-


-------
100.  Any other diseases of the
      nervous ay at ess
      Specify:
101.
102,
103.
104.


105.
106.
107.
108.
109.

110.
111.

112.

113.

114.
115.
116.
Anemia
Gastritis
Ulcers (stomach or duodenal)
Any other diseases of the
digestive system
Specify:
Cancer of the breast
Cancer of the stomach
Cancer of the esophagus
Cancer of the mouth/ tongue
Cancer of the large
Intestine
Cancer of the rectum
Cancer of the trachea.
bronchus or lung
Cancer of the liver or
biliary passage
Cancer of the bladder
or urinary organs
Cancer of the skin
Cancer of the thyroid
Leukemia
^** *>—iS
I) Q»o 2)0 Yes
1)0 No 2)0 Yea
D0 No 2)0Y«s


t)0Ho 2)QYes
x. — f v_«x
E)0No 2)0Ycs
1)0 No 2)0 Yes
1)0 No 2)0 Yen
I)0No 2)0 Yea
,, ^"^
1)0 No 2)0 Yea
1)0 do 2)0Yeo

1)0 Mo 2)0 Yea

1)0 No 2)0 Yes

1)0 No 2)0 Yes
1)0 No 2)0 Yea
l)0Ho 2)0 Yes
1)0 Mo 2)0 Yes
117.  Other cancer
      Specify;
118.  Mental retardation
                                                                                                            I)QNo     2)QYes

-------
For each of the diseasea you  have checked on the preceedlng two pages please complete the following tables:
Name of disease/
condition


Please list the nanc(e) of family
mcmber(s) with this disease/condition




Flcaoe list the name (a) and addrcssCes) of the
cllnic(s), liospttal(s) or doctor(s) consulted




Please give the date
of diagnosis





Name of disease/
condition
Please list the name(s) of family
member (a) with this disease/condition




Please list the name (a) and addreas(ea) of the
cilnlc(B), hoapltal(a) or doctor(s) consulted




Please give the date
of diagnosis





-------
For each of the disease* you have checked  on the preceedlng  two  pagee  pleese complete  the following tables!
None of disease/
condition


Please list the n«me(s) of family
aeaber(a) with thie diseaae/conditlon




Please list the naae(s) and adJress(cs) of the
clinlc(e), hospital (o) or doctor(a) consulted




Please give the date
of diagnosis




Name of disease/
condition
Please list the name (a) of family
ueober(s) with this disease/condition




Please list the nane(a) and addresses) of the
cllnlc(s), hospitel(s) or doctor(a) consulted




Please give Hip date
of diagnosis





-------
For each of the diseases you have  checked on Che proceeding two pages please complete the following tables!
Name of disease/
condition


Please list the name(s) of family
member (B) with this disease/condition




Please list the naoie(s) and address(cs) of the
clinic(e), hospltal(s) or doctor(s) consulted




Please give the date
of diagnosis





Name of disease/
condition
Please list the name(s) of family
member («) with this disease/condition




Please list the naaie(s) and sdJrcsii(cs) of the
clinlc(B), hoBpltal(s) or doctoc(s) consulted




Please give the date
of diagnosis





-------
For each at the diseases you have checked  on the preceedlng  two psgea  pler,sc complete the following tebledl
Name of disease/
coodlcioa


Please list Che nsme(s) of family
BCjeber(s) with thta disease/condition




Please list the nane(s) and address(es) of the
cllnlc(e), hospital (a) or doctor(s> consulted




Please give the date
of diagnosis





Name of disease/
condition


Please list the name (a) of family
member (s) with this disease/condition




Please list the name (a) and addrese(es) of the
clinlc(o)> hospital(e) or doctor(s) consulted




Please give the date
of dlagnoslo






-------
For each of the diseases you have  checked on  the proceeding two pages pleaee complete the following tables!
     of disease/
condition


Please list the narae(s) of family
member (a) with this disease/condition




Please Hot the namc(B) and addresa(eB) of the
clinic(o), hOBpltal(fi) or doctor(s) consulted




Please give the date
of diagnosis





Name of disease/
condition


Please list the name (a) of family
member (s) with this disease/condition




Please list the name(s) and address(es) of the
clinlc(s), hospltal(a) or doctor(s) consulted




Please give the date
of diagnosis






-------
ll.iv« you over licul any of the following
cyBptaas?

119. Severe fever
120. Extreme tiredness
121. frequent or very bad headaches
122. Problems with mouth or throat
123. Any problems with ears or hearing
Specify:
124. Any unusual difficulty with eyes
or eyesight other than a change
of the prescription of glasses?
Specify:

125. Sudden weakness or heaviness of
arms or legs?
Specify:
126. Numbness in arms or legs?
127. Swelling la arms or legs?
128. Stiffness In joints or bones?
Specify:
129. Pains in joints or bones?
Specify:
130. Spasms of 1 bobs?
131. Spells of dizziness?
132. Have you fainted or blacked out?
133. Very strong heartbeats?
134. Irregular (fast, slow or
Inconstant) heartbeats?

Ho


	




	




Yee





- -


._. .





How fri-qurnlly
does (or dlJ)
this occur?
(days, Booths,
or ycnrc)

















lluw lunB Jo
(or did) Che
synptosaB lest?
(deya, »onthE,
or yean;)


















Did you consult
a doctocf
Ho Tee
















- - -














Doctor's na»e 4 odd re si?

















Year no at
recently
experienced

















-------
Have, yuu ever hail any of tlio following

135. Have you dad pain, discomfort,
or trouble in or around your
heart?
136. Hcliing of tlic skin?
137, Any unusual discoloration or
eruptions on the skin?
Specify:
138. Any problems with your stomach
or digestive system?
Specify:
139. Swollen glands In your neck,
armpits or groin?
140. Have you lost 20 or more pounds
in the last five years?
(Include dieting)
No






Yen






How frequently
does (or did)
this occur?
(days, ttonclia,
or years)





X
How long do
(or did) the
eynptoua last*
(dtye, Bontha,
or years)







Did you
a doctc
No






i consult
rl
Tee






Doctor 'a name & address






Year most
recently
experienced







-------
  The following section is to be filled  out by the wives  of nfile fishermen/or/by fishervooen.   If  the wife  1* deceased, would  the husband complete
  thle lection to the best of bis knovledge.
141.  How raany natural, children  do  you  hsve? 	

      How cany step or adopted children do  you  have?

      Please list ell of the pretmanclea and outcomes of which you were Che father/mother  in  the sequence  they occurred.
Preg.
t
1.
2.
3.
.4.
5.
6.
Complications in
this pregnancy
(describe)






Outcome
Miscarriage






.Stillbirth






Live Birth






Other






Infants
sex
H F












•Date of Birth






Live
Birth
Height






Hospital, clinic or
piece of birth






Doctor's naae






I4la.  Have any of your natural children  died?   (do not  Include  stillbirths nor step or adopted children)

          1>ON°     2)Qlfes  
-------
142.  Do you know of any Dental  or  physical abnormalities at birth (birth defects) in yourself or your natural children.

             t)ONo      2)Qvc9    (If Yes, please complete the following table.)

                I
     (go to question 143)
1.
2.
3.
A.
iclatlonshlp to you
">clf Son Daughte












(Check appropriate boxes)
Uving




Dead




Nature of Birth Defect (describe)





-------
143.  Do you use e birth control  Bechodl    1>O *°     2)O'*«•   r")other (epecify)i
145.  At what age did you begin using  birth control methods?
146.  At what age did you begin  to menstruate? _______^_^_________
146a. Have you stopped neostruating?    I)("~)NO                2)C~~)^ea
                                (If Ho, go  to^questlon 149)
147.  At what age did you etop menstruating?  	
148.  Did the cessation occur naturally or  due to  eurgery?
       1) Q_) Naturally     2)^) Surgery  	fr Whet was tha reason for the surgery?_
                                                Doctor's name and address?	
                                                                          name                             aodresH
149.  What is (was)  the Average  length of your oenstrual cycle? 	dayo (Froo first day of bleeding to first day of next period)
ISO.  On the average,  how long  Is  (was) your  period?       days
151.  In the past five years, has  your blood  flow during menstruation  1) ^"^ increased?     7)^^ decreased?     3) C~} stayed the same?
152.  Have (or did)  you experience any abnormal  spotting or bleeding between your Eenstrucl cycles?  O^jNo     2)^") Yes (go to question
                                                                                          (go to question 154)
153.  If yes, did you  consult &  physician?  I)(~)NO     2}f"jYes     Doctor's name £ address
                                                                                              none                       sddreee
154.  Have you used,  or dldyou  u&e, any Dedications prescribed by a doctor for aenstrusl irregularities?
           I)    No     2)     Yes	>  NaBe of medication                                     ...	
Questions 1-140 have been answered  by
Questions 141-153 bave been answered  by
Thank you for your cooperation in  this health survey.  Please sign the consent fora and return the questionnaire In the enclosed self-addressed stamped
envelope.

-------
Reproduced from
best available copy
                                                                                         TwTN;p--^rr;,J'
                                                                                         $^*^<''sV.#"
                                                                                         <£?ti£^-:L-T> vV.

-------
                 Proxy  Questionnaire
                  FISHERMEN HEALTH  SURVEY

                o aid In our understand ing of  Mr;/Mra._
                                                 Instructions:                                           _ _
                                                 fishing habits and health, we ask that you complete the  following  quest-
                                                 ionnaire answering ttie questions as you believe he (she) would.  Answer
                                                 as  many questions. as possible from your knowledge of Mr./Kre.
      !»F oinru:Ti;i> BY OFFICE)
If you don't know how they would  have  answered  a question,  write "don't
know".  Start with question number  two.
I'roxy (nlt-rvlca >if Mr./Mrs.
llils proxy Interview  Is being completed  by:
'.''ill i-; i hi- rrlat lunshlp of  the  per Bon  couplet Ing  the Interview to Mr,/Mrs.


I.  AJdriHs of p-'rson completing  interview? 	
                                             Street
                                                         Relationship
                                             City
                                                                               State
                                                      Zip
ite    2) O Blabk     3)
1.  HI., i ; «;•» hls'hcr sex?       I)

3.  '.M,,, -  ...,., hi Whcr race?      i)

i>.  HlMt  u'.is lils/lior l»lrcl«lnte?
                                     month     day     year

r>.  Hh.it ".is his/her So<:i,il Security ituraber?

'..  Wl,.ii  w.is Ms/her martial status?     I )( — Jsinfcle (never married)
Hispanic
                                       «)'
                                                                                               Awericsn Indian      5) |~~I Other
                                                                                 Married
                                                                                                              or divorced
                                                                                                                                     Widowed
7.  IH.I tic/Shi? liol.l n current coranerclal  fishing  license?   1)C3 ^    2)1   JYes    Approximately over what years did he/she  hold  a  license? _ /

                                                                                                                                             frO1"  to
S.  Ui
-------
ID.   I ml liN
a= jitf/jtiu ever  s  n-eu m.-mlicr for, or ,1  pnrlnor wltli, an Indlvldunl owning a commercial fishing  liccnect
 I )[^  | il.i           Z)|   I Yen - ^ Ovot  wluit  years was lio/sfic a crew »embcr or pnrlnnr?       /
    I                                                                                      from    to

i If UK, |>U':>!ip  stoji licrr ami return tlic  questionnaire ns soon os poecllilc In the addressed envelope.   Thank you for your cooperation.)

ml liNiir wild ,111 "x" on llic enclosed nap  where lie/she commercially fished most often.  (You rany  Indicate more than one  location).

-------
"•   Was  ...rj.ocirl.il  fishing hid/her  I) |_j
     curr.nl  dil I-tine occupation?
                                                                         12.  Wliae wait  lita/tier  current full-time occupation?^
                                                                         13.   Has commercial fiohing ever his/her  full-tlae occupation?

                                                                                  2) L_J Yea ~* Over  what  years was ne/ahc full tl»e?      /	
                                                                                                                                      from  to

                                                                                  I) LjNo—*0ver what  yenrs vaa he/she   B part-tlBe'fiahernan?
                                                                                                        (go to  question  14)         	'	
                                             Ifes -*0ver what  years  did  he/she comaerclally fish full tine?       /_..-..
                                                                                                             from   to
                                                                                                                                      from    to

                                                                                                                             (go to question 14)
I'.. OiJ li.-,'jiln- >:urronily  own  a  s|>ort fishing license?  2)f~] Yes    t)t] No    15. Old he/she ever own a  sport  fishing license?  2)

                                                                                                                              I


|J.  Indicst.  wltli an "0" on the enclosed raap where he/she sport  fished  nost often.    (You Bay  indicate more than one location )
                                                                                                                                          Yes    1)Q **»

                                                                                                                                             (go to question  17)

-------
\l.   To  tlii-  ki'-'jl <>' v	  kni'wIrilKi', plr:i:ir  llstl I In- n:iinr!i  nnil nJdrcsM-M uf  hlx/hrr lull  or  pot l-i line crow nrnlii-ruT   (|>;i!iL  ;nil—-I  No  (lf  "°«  8° to «!"«-•« •"" 2fl )



 ft.   ,-,n.i..xlm.it.-ly  how nnny of  his/her meals contained fish caught  (by themselves or  a friend) from the Great Lakes?

             1) 	  |n-r  week         (of)   2)  _____  per moutn

 Ai.   ll.ivi mnuy "1 ilioso mrnls  Inclinlc the  following types  of flshi

           __r..Tln.on     	Walleye      	Hough Cisli      	Northern  Pike      	Lnke Trout     	Oass      ^	I'on fish


           	(itlior Trout	Perch              Burbot              JChuh       	Lnke Whlteflsli    	Smelt       	Lake Herring

               01  lu-i :  (r.|n-c I fy)	
 21.   A|.,-....«ira.ncly Imw n.iny  years did he/she  consume Great  Lokca fish with  thl« frequency? 	years


 ".   A,., r...ii,,;,i,.|y Imw in:iny  of  his/her meals  contained fish caught (by themselves or n friend)  fro- wotero other  than the Great  lakes?
              '>	!•«•«• »cck  (»c)	per  month
                  Reproduced  from
                  best available  copy.

-------
21.  How many  of  those meals Include  die  following types of fifth)

     	SaUon     	Walleye     	Bough  fish
         	Other Trout
                                       Northern  Pike
Perch
                                                          Burbot
                                       Chub
            	Oilier:  (specify)
 14.   Approximately how many yeare did he/she consume inland water fish with  this frequency?
 2*>.   II.>w often  illd his family members eat fish?
       l>n/L':                                             Sons!
       1)1  I As often  a»  he/she                        1)I   |  As often as he/she
       2) 1—I More  often thrtn he/she                    2)]   1  More often than  he/she
       1) I	I Less  often than he/she                    3) I   |  Less, often than  he/she"
       •'•) I	1 Doc.s  not  ply                             4) |	I  Does not apply
       •/ f.M »«'<'«T                                     5) O Never
Lake Trout
                                                         Lake WMtefish
                                                               years
                                                                                          Baas
                                                                                                            Pan fish
                                                                                     Smelt
                                      _Lake  Herring
                                                                                        Daup.htersi
                                                                                        1)1	L.Aa often  as  he/she
                                                                                        2)t.  1 Horc often than he/she
                                                                                        3)[_J Less often than he/she
                                                                                        1)(,	J Docs not  opply
                                                                                        5) 1   ) Never
 ^6-   .i|'|n i>xint.il r.ly how  m.iny pniinilo of fish  did he   consume  per year?
              (assume:   1  fish wciil equals 'j pound of  fleh)     .	pounds
 11.   Wh.it percentages of fish he/she caught and consumed were prepared by the following methods:
       I) Broiling	I
       2) I'.in frying 	Z
       'l) Smoko.l 	I
       I.) Hoi line	Z
       '») I'oochoil .	5!
       I.) Otlicr 	Z

-------
28.    W.i-; liu/shi- ••> clu.-irutlc smoker
      .•it  the time of dc:ilh?
                                                                          29.    How oji! w.is he/she when  he/ohe fleet began to smoke cigarettes?
30.   What was  the  average number of cigarettes he/she smoked per day?
                                                                          31.   How aany years did he/she smoke cigarettes with this frequency?
                                                                                                                                        (go to question
                       W.ir. he/she ever a cigarette smoker?    l)j  \ Ho    2)|  | Yea

                                                                I
                                        (1'f  No, go to question number 34)
                                                                                          32.    How old  was tie/she when lie/aha first began to smoke
                                                                                                cigarettes?
                33.   How many years did he/she omoke cigarettes? 	

                	                           (go to question  3$)

-------
I'l. W.i:i  lir/sho  a  pipe  smoker at
   I he  t l»c  of death?
I)
2)f~7lYes
                                        NX

                Via.  Was l.c/sl.e ever a pipe snokerT     l)C~]No
                                (If  Ho,  go co question number 40)
                                                                          35.  How old was he/she when he/she first began to smoke a pipe?
                                                                           36,  Hint Js the average number of plpefuls he/she sacked per day?
                                                                           37.. How many years did he/she smoke a pipe with this frequency?^
                                                                                                                                 (go to question 40}
                                                                                         3Q.   How old was he/she when he/she first began to
                                                                                               smoke a pipe?
                                                                                         3$.  How cany  years did  he/she  smoke  a  pipe?

                                                                                                                               (go  to question 40)

-------
          Reproduced from
          besl  available copy.
     the tlm,- jf rfr.illi?
           '•Oil.  W.is he/she ever a cigar smoker?   l)(  Jj No     2)1  1 Yea


                                                      i
                            (If No, go to  question number 46)
How old was  lie/she  when he/she began to omokc cigars?
                                                                                What IB the average number of cigurd lie/olio smoked  per day?
                                                                                How many years did  he/she smoke cigars with this  frequency?
                                                                                                                                   (go to question
                                                                                                   How old waa  he/she when he/site began to
                                                                                                   smoke cigars? 	
                                                                                                   How many  years did lie/she smoke cigars?
                                                                                                                                 (go to question 46)
*<•.   hi.I  liu/slir '-vet chew tobacco regularly?    Oi—J No      2){   JYes


47.   DiJ  he/-:l	WIT us,- snuff rry.ularly?   1)1   | No      2)|   \ tee


'•'J.  '(Mil  h<-/>ili'.- OVCT smoke non-tobacco products regularly?   1)J^	| "e     2)|^	jYes

-------
                           Hard  liquor  is considered to. IncludeJ  Gin,  Whiskey,  Bourbon, Vodka, Eu»e  Brandy,  or Scotch
                                     (A drink-1 to  l>i oz, of alcohol or  a mixed drink contelning this amount.)
*<).  w«s lnVslic a liard liquor     l)QHo
     .li iiiK-r ;it Uwr t li»o of
     ilc jili?
                               f
                                              Yea-
              ''(If  Ho,  f.o to question number 55)
                                                                50.  How old uae he/she when he/she  first  began  to  drink hard liquor?
51.  Hhat is the average number of hard liquor drinks he/she consumed
     per week?     	
                                                                 52.   How many years did be/she constant hard liquor with this frequency?
                                                                                                                               (go to question 55)
>>')n .  Vas he/she ever a hard liquor consumer?   1)L	iHo     2)|	J.Ves
                                                                                         S3>   Hou old was he/she when he/she first began to drink
                                                                                               hard liquorT
                                                                                            *•  lion raany years did he/she consume hard liquor drinks? _____
                                                                                                                                        (go to  question  55)

-------
>,  ',•!.>:: hc/shr n I.eer   I) |
   
-------
r.l.  '..'.is lie/she a wtnc
     Jr inker at the tine
     ni 
-------


Reproduced from J^
besf available copy. UsHjP
Dill (ltr/::hc) , -lih: or 'her PIHMISC
67.
Crt.
69.
70.
71 .

1 -,
/ .. .
73.


7 A .

73.
76,

77.
76.
7-J.
so:




az.
«i.
S', .
s-i.
Hf,.
ASttlln.l
Ri 'oiifhil 1::
ti»|.f.y;.-wi
TMl.orriilo:;l>:-TI>
Itoitoiim: 1 «i)!i 1 s-Hono-
Klsslllf, DIliCJIHC
v
Any tilhur tllncafio of
tin- respiratory
Specify:

Hep.it i rlj: or
yellow j.tnmllce
Cii r hunts
Any o t Itt-r 1 1 vCr
clir.rds.-
.'•PCC i I'y: 	 „„_
Spoitdyl il •:•
C..-,t
Mlii-iiHi.iiuiil An drli is
ll-;|.-...ir\ hfll Is

.-..-.»
Itottt": t
i— ___| HO

nO NO
f— ,.^
DLU No
DC] NO

on NO
DtU No

n CD NO
nCZJ NO



l)C3 No
i) cm NO
DCU No
I) CD No
l)tZ3 No
1)1 I No


his/her children ever
2) IZ3 Yea
2) EU Yes
2)C3 Yea
2)C3 Yes

2) d Yes
1 — 1
2) L~~*J Yes

2) CD Yes

2) CD Yes
2) CH Yes

2) \ Ij Yea
2) L3 Yea
2) CJ Yea
2) CD Yc.3
2) CD Yes



2) CD Yea
2) tZI *«•
2){~~I Yes
2)CU v«
2)Q »«•
2)CU Yes


luwe any
07.

88.


89.

90.
91.
92;
93.

94.
95.
96.

97.
98.
99.

100.


101.
102.
103.
104.



of the following diseases or
Heart attnch-MI or coronary
stroke-cerebral accident
(CVA)
Hemorrhage- thrombosis-
embollsm-ony other disease
of heart
Or citculotory system
Specify:
Diabetes
Thy ro idle Is
Any other glandular disorder
Specify:
Rye diseases
Specify:
Psoriasis
Eczema
Herpes zoster-shingles
(dermatitis)
Pcmphtnr.»8
Any other diseases of skin
specify:
Multiple. Sclerosis

Any other diseases of

nervous uystom
Specify:
Anemia
Gastritis
Ulcer«(stom,ich or duodenal)
Any other dinenne." of Che
digestive system
Specify:


conditions.

l)Cm No


l)f~1 No

1)1 1 Ho
onu NO
1)D No
t)dHb

ntm NO
1)CU No
D CD No

n cm NO
O CD No
on NO
ndJ NO




D 	 1 No
1) J Ho
nUD NO
1) { 1 No

nl~~t NO


(Please check appropriate box)

2) i 	 1 Yes


2) QYes

2) {^JYcs
2) CU Yes
2) ["""{Yen
2) CJ Yes

2) £3Dves
2) j 	 /Yes
2) | 	 JYca

2) f~~| Yes
2) f~~l Yrs
2)r— jvcs
2) m Yes



2) LH Yes
2) r~~|Ycs

2)C3^'

2) i 	 | Yes

-------
105.
106.
107.
108.

109.

110.
111.

112.

113.

114.
115.
116.
117.

Cancer of the breast
Cancer of the stomach
Cancer of the esophagus
Cancer of the mouth
or tongue
Cancer of the large
intestine
Cancer of the rectum
Cancer of the trachea.
bronchus or lung
Cancer of the liver
or biliary passage
Cancer of the bladder
or urinary organs
Cancer of the skin
Cancer of the thyroid
Leukemia
Other Cancer
Specify:
Dp~) No 2) CU Yc»
0 LTD -NO 2)[
l)QNo 2)1

OtHjNo 2)]

nlHl No 2)
l)CZj No 2)

HI Yea
HjYes

ID Yea

LjY6ff
[YCS

I>C J Mo 2) CHI Yes


l)CHI No 2) 1 jYes

l)I~"t No 2)
nl~l HO 2)
l)CHNo 2)
nCUNo 2)1


~~]Yes
ZU Yes
j Yea
~~| Yes

I'll 1 Un 71 f i Yon

-------
        For each of die diseases you have  checked on the proceeding two pages please complete  the  following tables:
Name of disease/
condition








I'lea-x: 1.1 nt the nai'ie(r>) of family
member (n) with lliln tl 1 r;i:n'ic/coiul 11 Ion






Ploncc .Mat. Clio MOIHC(B) and nJdreBs(ea) of tl>o
clinic (ft), lioBpJtnl(n) or Joctor(s) conoiiltetl






Please give tl'C Jntc
of OlngnoBls





 Karae of disease/
 condition
list the nau
-------
, i	i... .11 :..;••!.-» you imvr.  chccko! on the  prceeeJinn two pngcn  please coraplete  tlic following


I'lc.inc list the nonc(s) of family
mi-mliri (r.) wlili thin il l.icnsc/cond ttlon




Please list the ooeseCo) and ad
-------
II,.- ,11:
yon tn»vt>. clmcknl  «>i>  tl>ti
                                                    tvo pnacii ulcnoc complete the folloulnR tnlilent


I'lc-Tr.c Vlst llic unmc(r.) of family
mi ml. cr(.':) wllli tlilfi dlncnoc/<*'«vJltiou




ric.iBc list tlic nnmc(p) and ndclroBs(en) of the
cllnlc(s), hoo|)ltnl(B) or doctor (s) consulted




Plcnsc give the Jntc
ol Ulaynosie













rl<-:i-u- 1 IKI the nnn<:(i;) of tnmlly
i»<.-int>rr(r.) with tills it 1 Pcasc/con.) 11 Ion






ric.noc Hat Lite name(B) nn«- f,ivc Clio 
-------
i	„ I, ..I  it,. ,11,.,-.,•!,••.  ymi  |,ntfi<  dtrtTkril I'll llul pr«!t:ufillt»n  two  |inRrii |.|rnnc  c««|ilr'n  tlio  follnvinR  Utitaot


I'lcnno list tlio namc(s) of family
ntvmlx-i (R) wttli tliln d Isonsc/condltlon




Please Hat the nanc(s} and adclreas(ea) of tli«
cUnlc(B), tK>8pltal(a) or doctor (s) consulccd




Please give tlic date
of diagnosis







l'li':i>«- lint the iinmi:(n) of Cninlly
«. tolicr (r.) wJtli this disease/condition





PJcnso Use Clio na«e(n) and nildrcssCos) at the
clinic(s), lioapital(n) or doctor(s) consnlte
-------
I 1:1.  H..V iii.iitv .-liil.li.-it .11-1 lir/.ilii.  IMV.-Y  ( ___ iiiiliirnl  clilliiiru)   (ind  {
                                                                                            iili-ii ur mluplcd  children)
                  rii-.-ir:.-  ! l::i  :i 1 1  (In- |.r c|.|i.tuc I on nnd  nnCconcn of  which lic/sho wns the  C nl licr/raotliec  In the acqucnce they occurred.
I'l vgttaticy :;.M|m:tn c
IIIIMllx' 1
1
?
1
/,
S
6
Ulnc.-irrlnr.c






StlHblrl.li






Live nirth






Other






Infnuta
Sex
H F












Date of Dlrth






Ltve
Dirth
Weight






Iloepitol, Cllnicfor
plnce of birth






Doctor's Nnme






119.   ll.-ivi- any of  his/her natural  children dted?  (do not  include sttllblrfhs nor  step or adopted children)




                1) |	J Ho      Z) [   | Yeo   (I£  yee, plcnsc complete  the following table.)
..JL


nx
r




n.itc o( nirth




Dote of Death



;
Cause of Death




City and State in which dcnth wan reported





-------
i.-«.  Ki >..u  tiiuw ut miy niciilitl in  (.liyiitcol aliMonunUtiea ot birth (birth defects)  in  him/her  or  his/her natural children.
                        1) |   |  Ho     2) 1   | Yeo   (If yen, pleoee  complete the following table.)
(go to question 121)
He I.It
lllciirlf/lict.iylf



lonnjijp
Soil




to lilra/hcr (check approprloce boxes)
D.THP.IltOl




Living oc Jcatl
	 —






Nature of birth Defect (describe)





-------
Ill 1 t.r


I :i . SevKre fevci
fU. €it»O"i! t 1 redness
1 11. 1
.:«. •
1 iS. A
t,
i oouful ur vcty li^il hi..;tilnchrn
rol»U-n>s ullli uuiiit h uf Itiruut-

I'fCIFT:
' 26 . Auv unir:iia ) (1 1 1 f 1 1:11 1 (.y with ryes or
eye', if.'" otlii'i III. in ,1 cli.mc.e of the
pi c sc r 1 |»l 1 mi i>f p. l.i.ssrn?
Sri C I l-'v :
117. Sudiirit wr;iliiiir!in or lic;ivliiCRfi of
.lin:-! LI ll-|'.:.''
1 -1 . 1
luiiiltii.':;:) In .-il'mrt nr lop,«?
>%J«- I 1 1 IIJ*. Ill .irill!: 1M' 1c|',!>?
HO. S< i 1 f IHT-.S In juliils or Imiicn?
srttirv:
1 \l .
'.lin^ In |(iliil:: or liunr:;?
1 12. S,,.r:i.i-: of 1 Iml.s?
IJ'- Hut l.o/shc: li.'iv spells of dizziness?
It/,. IM'I >iu/';ho f.ilul ur lilack out?
IIS. Vcvv slrouf, hour Ideal K?


Reproduced from
best available copy. ^^^
Vlii|il 	 I
No
	
—











Yon



	











li>M 1 | c'|lli'||| |y
Mil L|i In mciifl
(days, monChs,
or years)
















lluw IUHK illit
I lir nyin|il umti
Inst? (days,
moot lie or
years)

















Did lie/she
consult a doctor?
No Veo
































Doctor's namo & aOJrcsa
















Ycnr monr/
recently
cxpprl'rnc.

















-------

-• ... . ...



ll(*. 1 1 1 » I'.ii f.-ii {f.vcl, Hltiw, nr
in. .Hi-ii .nil ) li«':irl lii-.ie riT
IU. lilil li /sl.c li.ivc pain, discomfort, or
irmililt' In or around Che heart?
MH . 1 1 • li 1 il j; M| 1 IIP r.k t II?
1 >o . ,\nv niiii'iii.'i t il I uc«» lornl 1 «»ii or
« i ii|»i l.«ii:t (>ii (In: nktn?
: ri I'll V:
l'ii|. An-/ (inililtins utth i lie scomnch or
d Ir.ni -:l IVP Kystcni?
:•!'< '-I 1 i :
|.',l . Swi.ll.-ii f.l.in
1 —
i or
1° I
IT) 1
j~< j
w



Ho
























.Yen





















II. iu ( I 1'ijMi'ilt |y
illil 1 lilii ni-i-iii 7
(days, Months,
or years')






















llnw IIHIH illil
( In* iiy«|
-------
  U.,  i',, i i. .,.(,„•.-  ., , i Inn  In  lo  In- I Illi-it nut  |.y  I lit- wile til miilr Htiliriuii'n/or/by ( lnluTuomcii ,   If tin; wife In ilcccmiwl ,  Winild you complete tliln in > I Inn
  >» i In- I   -I  nl  youi  kimwlnlc.r :i hi ml the  practices o£ the 1 luliurraau'u wife.




145.  U,,  K|,t.  us.-  ;i Mrtli control method?   1)0"°     2)d]Ye8  (If yen, answer questions 144 and  145}


liA.  Ulinl  iiii-lliii'l "f til rlli run! rnl   What una  the rcnson for tlic  surgery?


                                                   Doctor's  name 4 address?
                                                                                  :                           address

 tl*  Wi.ji  11,  («.•.!>  I In- .ivi r.-i|-,L-  li-u^tli of  her menntrunl cncle? 	days'(From the first  day of bleeding  to the first day of next period.)


 i'.)   On  t h.- :iw-rjp,e,  Imw  Innp.  Is (w;)s)  l(er  period?  	(dnys)


 iSi.  in.ti.n I..-IKI  Mvr y,-..,.;,  liuillicr  blood flow during ncnst ruat Ion I.C3 Increaned?    I (U decrenaed?    3 tZJ stayed the same?


 1 Tit   Ha.-e (IT illil) r.lie   i-xpei Irnrn any .iliiinrm.il spotting or bleeding between her  menstrual  cycles? l|^  j  Ho   2J    \  Yea (go  to question  153)

                                                                                                        (go to  question 154)
 •'•>•  l<  yv., .11.1  si,,-  imisuK  .-, phynlclan?) 1 .Q No 2-.Q ye8      Doctor's name & address	
                                                                                                   home                      address
        M.I.J  ;l«  •.:.,!.  ,,r .11,1  „;<• ,,nc. niiy  «e
-------
Respondent ID No. 	/	/	/	/_
Date













Day of
Week













Results Codes
Intervj
Refusal
Unavail
ew complet
Time













Result of
Contact
(Enter Code)













Languag
e .... 01 Decease
..... ft? Rdcnnilrl
able 	 03 Ihcompe
Comments













e problem .... 04 Apointment m
d 	 .05 Call back n<
ent moved .... 06 No answer, n
tent 	 07 Line busv .
Interviewer
ID No.














) appt . made . 09
o one home. . .10
	 11

-------
           Telephone Interview
              Large Cohort


GREAT LAKES  FISHERMEN  HEALTH SURVEY
                    Interviewer Name
                    Interviewer ID No
                    Respondent ID No 	/	/	/	/	

                    Date of  Outcome  __/__/	


                    Interview Time Began	a.m./p.m.

                    Interview Time Ended	a.m./p.m.
       University of Minnesota
       Division of Epidemiology

-------
                                GREAT LAKES FISHERMEN HEALTH SURVEY
 I  WOULD LIKE TO BEGIN BY ASKING SOME  GENERAL  QUESTIONS ABOUT  YOURSELF,
1.  First, what is your current address?
                                        street
                                        city                           state      zip
2.  What  is your birthdate?   	/	/	
                            month     day   year



3.  What  is your current marital status?    I. f) Single (never married)
                                         2. f  \  Married

                                         3. (  )  Separated or divorced

                                                 Widowed
4.   How many children  do you have?	 Sons    	 Daughters

-------
5.  Do you presently hold a commercial fishing license?

    1. C j No  2. ( ) Yes 	:> Approximately how long have you held this license?

                                 (PROBE FOR DIFFERENT PERIODS OF TIME)
                                     _
                                from  to
                                            from to
 from  to
                                                                                No. of years



                                                                                 GO TO QUESTION 8
6.   Did you ever hold a commercial  fishing license?

    1.    \ No.  2.     Yes 	> Approximately how long did you hold this license?

                                (PROBE FOR DIFFERENT PERIODS  OF TIME)
                               from  to
                                           from   to
from  to
                                                                              No. of years



                                                                                 GO TO  QUESTION  8
7.   Have you ever been a crew member  for, or a partner with, an individual owning a commercial  fishing license?

    1. T 'j No  2. C  \ Yes 	:>How long have you been a crew member or partner?  	


                               (PROBE FOR  DIFFERENT  PERIODS  OF TIME)

                              	/	      /           /
                                                                            No. of years
                              from  to
                                          from  to    from  to
    IF  NO, STOP THE INTERVIEW.   THANK THE RESPONDENT FOR HIS COOPERATION  AND EXPLAIN THAT

    CURRENTLY  WE ARE ONLY INTERVIEWING  PEOPLE WHO HAVE COMMERCIALLY FISHED.
                                                  — 2—

-------
 8.  Near what  towns do (did) you  commercially fish most  often?
          city	  state             city	state
8a.  Where is  (was) your hailing  port?
          .city                      state
 9.  Is commercial fishing your current full time occupation?
     1. ( ) No  2. \~\ Yes 	•> How long have .you commercially fished full  time?
                                (PROBE FOR  DIFFERENT PERIODS OF  TIME)      N°' °f
                                from  to     from  to     from  to
                                GO TO QUESTION  12
10.   What  Is your current full  time occupation?   (PROBE FOR TITLE,  DUTIES  AND PLACE OF EMPLOYMENT)
     title/duties                                                     place of employment
                                                   -3-

-------
11.   Was commercial fishing  ever  your  full  time occupation?

     1.  O No  2.      Yes 	>How long were you  full  time?      (PROBE FOR  DIFFERENT  PERIODS OF  TIME)
                                 No.  of  years


      liow  lo.ng have you been a part time commercial fishermen?   (PROBE FOR DIFFERENT PERIODS OF TIME)
      No. of years       from  to     from  to     from  to
 12.  To  the best of your knowledge, tell me the names and addresses of full or part time crew members.
     (Past and present).                                                                         Employment

     name	  st reet, cit ; , state, zip, plione number	   No. of years,  from - to
                                                      -4-

-------
In  this section  I am going to ask you some questions  about  your fish consumption habits.
13.   Approximately how many times do you  eat  fish from the Great Lakes?
     1.  	 Never                   5.  	 < 1 time/month
     2.  	 1-2 times/week          6.  	   1 time/month
     3.  	 3-5 times/week          7.  	 2-3 times/month
     4.        6-7 times/week
14.   Approximately how many years have  you consumed Great Lakes fish with  this frequency?
                                                                                         No.  of years
15.   On the  average, how many pounds  of  fish do you consume per meal?
                                                                      Pounds of fish
16.   What  types of Great Lakes fish  do you eat most often?
     	 Salmon      	 Burbot         	 Northern Pike     	 Smelt            	 Pan Fish
     	 Perch       	 Rough Fish     	 Lake Wliitef ish    	 Bass             	 Other  Trout
     	 Walleye     	 Chub          	 Lake Trout        	 Lake Herring     	 Other  (specify)
                                                     -5-

-------
17.   In comparison to  yourself, how often does your family eat fish?
     Wife;                                                 	
     1.   	 As  often  as myself                          1.  	
     2.   	More often than myself 	 How often?     2.  	
     3.   	 Less often than myself 	 How often?     3.  	
     4.   	 Does not  apply                              4.  	
     5.   	  Never                                      5.  	
     6.         Don't know                                  6.
                                                      As often as myself
                                                      More often than myself
                                                      Less often than myself
                                                      Does not apply
                                                      Never
                                                      Don't know
                                                      How often?
                                                      How often?
     1.
     2.
     3.
     4.
     5.
     6.
As often as myself
More often than myself
Less often than myself
Does not apply
Never
Don't know

How often?
How often?



1.
•2.
3.
4. _
5. _
6.
As i
Mori
Les:
Doe
Nev'
Don
                         As often as myself
                         More often than myself
                         Less often than myself
                         Does not apply
                                       How often?
                                       How often?
     1.
     2.
     3.
     4.
     5.
     6.
As often as myself
More often than myself
Less often than myself
Does not apply
Never
Don't know
How often?
How often?
1.
2.
3.
4.
5.
6.
As often as myself
More often than myself
Less often than myself
Does not apply
Never
Don't know
How often?
How often?
                                                     -6-

-------
 In the next  section,  I would  like  to ask you some  questions about your  tobacco  and alcohol use.
 18.   Do you smoke cigarettes now?




      1.  ~} No  2. f~~  Yes
19.   How old were you when you first began  to smoke cigarettes?
                                  20.  What is the average number of cigarettes you presently smoke per day?




                                  21.  How many .years have you smoked with this frequency? 	




                                       GO  TO QUESTION 25
18a.   Did you ever smoke  cigarettes?




      1.     No  2.     Yes
      GO TO QUESTION  25
   22.  How old were you when you first began  to smoke cigarettes?




   23.  How many cigarettes did you smoke per  day?	




   24.  How many years did you smoke cigarettes? 	



        GO TO QUESTION 25
                                                    -7-

-------
 25.  Do you smoke  a  pipe now?
      1. (~\ No   2. O Yes
             Y
25a.  Did 'you ever  smoke a pipe?
      1.  (~\ No  2. C  Yes
 32.  Do you. smoke  cigars now?
      1. (~) No  2. (""") Yes
32a.   Did you ever  smoke cigara?
      1.      No  2.     Yes
             V
       GO TO QUESTION  39
26.  How old were you when you first began to smoke a  pipe?
                                   27.  What is the average number of pipefuls you presently smoke  per day?
                                   28.  How many years  have you smoked a pipe with this frequency?  	
                                         GO TO QUESTION 32
29.  How old were  you when you first began to smoke a pipe?
30.  How many pipefuls did you smoke per day?  	
31.  How many years did you smoke a pipe? 	
     GO TO  QUESTION 32
33.   How old were  you when you began to smoke cigars? 	
34.   What is the average number of cigars you presently smoke per  day?
35.   How many years have you smoked cigars? 	
      GO TO QUESTION 39
36.  How old  were  you when you began to smoke cigars?
37.  How many cigars did you smoke  per day?  	
38.  How many years did you smoke cigars? 	
     GO TO QUESTION 39
                                                   —8—

-------
39.  Have  you ever chewed  tobacco regularly?
     1.    \ No  2.     Yes
40.   Uave you ever used  snuff regularly?
     1.    \ No  2.    Yes
HARD  LIQUOR  IS CONSIDERED TO  INCLUDE:   Gin,  Whiskey,  Scotch,  Vodka,  Rum, Brandy.
                                            (One  drink  =  1 to  l>s ounces  of alcohol)
41.   Do you drink hard  liquors now?
     1. (~J No  2. (""")  Yes 	^
   42.   How old were you when  you- first began  to drink hard liquors? 	
   43.   What is the average number of hard liquor drinks you have per week?
   44.   How many years have you consumed hard  liquor with this  frequency? 	
        GO TO  QUESTION 48
            M
4la.   Did you ever drink  hard liquors in  the past?

      '•O
No  2.
                     Yes
           \
     GO TO QUESTION 48
45,  How old were you when  you first began  to drink hard liquor?
46.  How many drinks did .you have per week? 	
47i   How many years did you drink hard liquor?
     GO TO QUESTION  48
                                                 -9-

-------
48.  Do you drink beer now?
     I.     No  2.  "  Yes -
             \

48a.  Did you ever drink beer?
      1. O No  2. (  } Yea
            v t
55.  Do you drink wine now?
     1. ~\ No   2.     Yes
           \l
      GO TO QUESTION  55a
                                   49.  How old were  you when you first began to drink beer?
                                  50.  What is the average number of beers (12 oz. bottles)  you  presently drink
                                       per week? __	
                                  51.  How many years  have consumed beer with this frequency? 	
                                        GO TO QUESTION 55
                                  52.  How old were you  when you first began to drink beer?
                                  53.  How many beers did you drink per week? 	
                                  54.  How many years did you drink beer? 	
                                        GO TO QUESTION 55
                                   56.  How old were  you when you first began to drink wine? 	
                                   57.  What is the average number of wine drinks (4-6 oz.  glass)  you  have per
                                        week? 	
                                   58.  How many years have you consumed wine with this frequency? 	
                                         GO TO QUESTION 62
                                                    -10-

-------
55n.  Did you ever drink wine?

     1.    No  2.    Yes
     GO  TO QUESTION  62
59.  How old were you when you first began to drink:winet
60.  How many wine drinks did you have per week? 	

61.  How many years did you drink wine? 	
     GO TO QUESTION 62
 In  this section I would like to ask  you  some medical  conditions you or  your family may  have
 had.   Please tell me  if youf your wife,  or children have ever had any of  the following
 conditions or diseases diagnosed by  a  doctor.





Disease
62. Asthma
63. Bronchitis
64. Emphysema
65. Tuberculoais-TB


1-No
2-Yes
3-Don't
Know




FnmLly
Member
S-Self
W-Wife
Son
D-Dmighter








Name & Address of Clinics,
Hospitals, or Doctors Consulted








Dote of
Diagnosis




                                              -11-

-------


66.
67.
68.

69.
•70.
71.

72.
73.
74.
75.
76.

77.
78.

Disease
Mononucleosls-Mono-Kissing
Disease
Pneumonia
Any other disease of the
respiratory system
Specify:

Hepatitis or yellow jaundice
Cirrhosis of the liver
Any other liver disease
Specify:

Spondylltis
Gout
Rheumatoid Arthritis
Osteoarthritis
Any other diseases of bones
and joints
Specify:

Meningitis
Hypertension or high blood
pressure

1-No
2-Yes
3-Don't
Know
















Family
Member
S-Self
W-Wife
Son
D-Daughter

















Name & Address of Clinics,
Hospitals, or Doctors Consulted

















Date of
Diagnosis
















-12-

-------


79.
80.
81.
82.
83.

84.
85.
86.

87.

88.
89.
90.
91.

Disease
High cholesterol
Angina pectoris
Heart attack-Mi or coronary
Stroke, cerebral accident,
(CVA) -hemorrhage, thrombosis,
embolism
Any other heart or circulatory
disease
Specify:

Diabetes
Thyroiditis
Any other glandular disorder
Specify:

Eye disease
Specify:

Psoriasis
Eczema
Herpes zoster-shingles
(dermatitis)
Pemphigus

1-No
2-Yes
3-Don't
Know
















Family
Member
~S=Seir
W-Wlfe
Son
D-Daugliter

















Name & Address of Clinics,
Hospitals, or Doctors Consulted

















Date of
Diagnosis
















-13-

-------
                  Disease
                                  i-No
                                  2-Yes
                                 3-Don.'t
                                  Know
  Family
  Member
 ~S-Self~
  W-Wtfe
    Son
D-Daughter
   Name & Address of Clinics,       Date of
Hospitals, or Doctors Consulted    Diagnosis
 92.


 93.

 94.



 95.

 96.

 97.

 98.



 99.


99a.

100.

101.

102.
Any other diseases of skin
Specify:	
Multiple Sclerosis
Any other diseases of the
nervous system
Specify: 	
Anemia
Gastritis
Ulcers (stomach or duodenal)
Any other diseases of the
digestive system
Specify: 	
Have you, your wife, or
children ever had cancer?

If yes, what location?
Leukemia
Hodgkin's Disease
Other Cancer
Specify: 	
103.  Mental retardation
      Any other disease
      Specify:. 	
                                                       -14-

-------
In this next section I am interested only in symptoms you may have experienced.
you ever had any of the following symptoms?
Have


Symptom
105. Severe fevor
106. Extreme tiredness
107. Frequent or very
bad headaches
108. Problems with
mouth or throat
109. Any problems with
ears or hearing
Specify:


110. Any unusual diff-
iculty with eyes
or eyesight other
than a change of
the prescription
of glasses
Specify:


111. Sudden weakness
or heaviness of
arms or legs?
Specify:


i ,.M~
2-Yes
3-Don't
Know












How often does

(PROBE for times
per day , times
per week, etc. )












How long did the
symptoms last?
(PROBE for hours,
days, weeks,
months or years.)












Did you
conoull
n doctor!
iun
2-Yes
3-Don ' t
Know














Doctor's nnme
ond address














Ycnr most
recently
experienced












                                           -15-

-------


Symptom
112. Numbness in arms
or legs
113. Swelling in arms
or 'legs
114. Stiffness in joints
or bones? Specify:

115. Pains in Joints or
bones? Specify:

116. Spasms of limbs?
117. Spells of dizziness
118. Have you fainted
or blacked out?
119. Irregular, fast or
slow heartbeats?
120. Have you had pain
discomfort, or
trouble in or
around your heart?


i-No
2-Yes
3-Don't
Know











How often does
(did) this occur

(PROBE for times
per day , times
per week, etc.)











How long did the
symptoms last?

(PROBE- for hours
days , weeks ,
months or years)











Did you
consult
a doctor?

1-No
2-Yes
3-Don't
Know













Doctor's name
and address













Year most
recently
experienced











-16-

-------


Symptom
121. Itching of the skin?
122. Any unusual dis-
coloration or
eruptions on the
skin? Specify:

123. Any problems with
your stomach or
digestive system?
Specify:


124. Swollen glands in
your neck, armpits
or groin?
125. Have you lost 20
or more pounds in
the last five years?
(include dieting)


1-Ho
2-Yea
3-Don't
Know








How often does
(did) this occur

(PROBE for times
per day , times
per week, etc.)








How long did the
symptoms last?

(PROBE for hourfe
days, weeks,
months or years)








Did you
consult
a doctor?

1-No
2-Yea
3-Don't
Know










Doctor's numo
and address










Year most:
recently
experienced








126.   Finally,  what  is  your  race?
1.  White
2. Black
3.  American Indian
l*. Other:
                                                    -17-

-------
The  last section of the questionnaire  relates to  your wife  and children.   If I could speak  to
your wife, she may be able  to answer the few questions remaining.  Thank  you very  much for  your
time and cooperation.
 What is your first name?
 127.  How many natural children do you have?


 128.  How many step or adopted children do you have?


 129.  please tell me about all pregnancies and outcomes of which you were the mother/father  in the sequence
      they occurred.  I am interested in your current marriage only.
Preg.
No.
1
2
3
A
5
6
Complications in
this pregnancy
(describe)






Outcome
Mis-
carriage






Still-
birth






Live
Birth






Other






Infants
sex
M






F






Date of
Birth






Live
Birth
Weight






Hospital, clinic
or place of birth






Doctor's
name






                                                    -18-

-------
129a.   Have  any  of your natural children died?  (do not include stillbirths nor step or adopted  children)
1
GO TO QUESTION 130

Sex
M F
I
2
3
A
Date of Birth




Date of Death





Name of Child




Cause of Death




City and State in
which death was reported




 130,   Do  you  know of any mental or physical abnormalities at birth (birth defects)  in  yourself  or  your natural
       children?
       1. C j No   2. () Yes   (If yes, please complete the following table)
       GO TO  QUESTION 131
             CHECK APPROPRIATE BOXES
Relationship to you
Self



Son



Daughter



Living



Dead



Nature of Birth Defect (describe)



                                                    -19-

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 131.   Do you use a birth control method?

       I .    No  2 .  ~\ Yes  - >  fiO TO QUESTION 132
131a.   Did  you  ever use a birth control

                 2.QYes
      GO TO QUESTION 134



 132.  What method of birth control have you used  the most during your adult life?

       1. r^) pill              5. (~~\ rhythm

      2. f~^\ IUD               6. fj condom

      3. fj diaphragm         7. f J other (specify)	

      4. ^ J foam or jelly



 133.  At. what age did you begin using birth control methods?	
 134.  At what age did you begin to menstruate?



 135.  Have you stopped menstruating?

      1. O No  2.     Yes
              V
      GO  TO QUESTION 137
                                                     -20-

-------
136.   Did the cessation occur naturally or due to surgery?




      1.  "   Naturally  2. f~~\ Surgery
                           What was tlie reason for the surgery?




                           Doctor's name and -address
137.  Have you taken any medications (since that time)prescribed by a doctor?




      1. """  No  2.  *~^\ Yes 	3» What medications are these?
138.  What is (was)  the average number of days between periods?
              _days
139.   On the average,  how long is (was)  your period?
days
140.   In the past five years,  has your blood flow during menstruation
            increased?




            decreased?




            stayed the same.




            does not apply.
  L.   Have (did)  you experience(d) any abnormal spotting or bleeding between your menstrual cycles?




      I. C~^\ No  2.  ("} Yes
             V
      GO TO QUESTION  143
                                                    -21-

-------
142.  Did you consult a physcian?




      1.  C j No  2. C j Yes 	:>• Doctor's name and address:
143.  Have  you used or did you use any medications prescribed by a doctor for menstrual irregularities?




      1. r~\ No  2.   *\ Yes 	>  Name of medication
That  was my  last question.   Thank you very much for  your time and cooperation.
                                                  -22-

-------
FILL IN IMMEDIATELY AFTER COMPLETING THE. INTERVIEW:
I.  Questions 1-126  have been answered by
2.  Questions 127  -  143 liave been answered by
3.  The overall  quality of the interview was:
1.   Very good




2.   Good




3.   Fair




4.   Poor
4.  How reliable  do  you feel the respondents'  answers were?   	 1.  High quality




                                                            	 2.  Questionable




                                                            	 3.  Generally reliable




                                                            	 4.  Unreliable








    Interviewer's comments:
                                                   -23-

-------
         APPENDIX III
Pilot Study and Survey Materials
       Great Lakes Study

-------
                                Contents


1    Introductory letter Protocol I

2    Introductory letter Protocol I - Proxy

3    Introductory letter Protocol II

4    introductory letter Protocol II - Proxy

5    Introductory letter Protocol III

6    Introductory letter Protocol III - Proxy

7    Great Lakes Study Consent Form

8    Great Lakes Study Consent Form Without Medical Records Request

9    Great Lakes Study Consent Form - Proxy

10   Great Lakes Study Consent Form Without Medical Records Request - Proxy

11   Second Request for Consent Form

12   Medical Records Request Letter - Doctors

13   Medical Records Request Letter - Clinics

14   Medical Records Request Letter - Hospitals

15   Second Request for Medical Records

16   Medical Records Abstract Form

17   Validation Form

18   Death Certificate Request Letter

19   Death Certificate Abstract Form

20   Study Participation Request Letter  (appropriate for individuals with
                                          no phone or unlisted numbers)

21   Study Participation Return Postcard

22   Set of Questions   (appropriate for Protocol II Participants

-------
The following materials were developed for use in the Survey of the
Large Cohort of Commercial Fishermen.

23  Respondent Information Sheet

24  Interviewer Assignment Record

25  Definitions (appropriate for questioning regarding health histories)

26  Survey Manual - Great Lakes Fishermen Health Study

27  Non-interview Report

-------
         UNIVERSITY OF MINNESOTA
         TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis, Minnesota 55455
Dear Fisherman:

     The sport and commercial fishing industries of ..the ..Great
Lakes region are of  significant economic importance  to  the Great
Lakes states.  The health and"well-being of the members  of the
fishing industry are important to their families as  well as to
the economy of the Great Lakes Basin.
     In 1978 our "Great  Lakes Project" was initiated to  evaluate
the health of those  individuals involved with sport  or  commercial
fishing in the Great Lakes.   It is our understanding that you have
either served on a commercial fishing vessel or have possessed a
commercial fishing license.
     In several days an  interviewer will contact you -by  phone .to
ask you questions regarding  your fishing practices and  health
status.  Your answers will be kept confidential.  This  is a medi-
cally-related survey and we  uphold your right to privacy.
     Enclosed with this  letter you will find a medical  consent
form.  The interviewer will  explain its purpose and  answer any
questions you may have regarding this health survey.
     Thank you for you cooperation.  Should you have any questions
please call the study office, collect (612) 376-8775.
                                 Sincerely yours,
                                               i. I :
                                                   * I /' . :
                                 Leonard M. Schuman,  M.D.
                                 Professor and Director
LMSrkb
encl.
                             HEALTH SCIENCES

-------
         UNIVERSITY OF MINNESOTA
         TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
     The sport  and'commercial fishing industries  of  the Great
Lakes region are  of significant economic importance  to the Great
Lakes states.   The  health and well-being of  the members of the
fishing industry  are important to their families  as  well as to
the economy o.f  the  Great Lakes basin.
     In 1978 the  University of Minnesota initiated a "Great Lakes
Study"-to evaluate  the health of those individuals involved with
sport or commercial fishing in the Great Lakes.   It  is our under-
standing that Mr./Mrs.	 served  on a,  commercial fishing
vessel or held  a  commercial fishing license.
     In several days an interviewer will contact  you by phone to
ask you questions about the fishing practices and prior health
status of Mr./Mrs.	.  We will  ask that you answer
our questions as  you believe Mr./Mrs.	_would.  Your
answers will be kept confidential.  This is  a medically-related
survey and we uphold your and Mr./Mrs.	's right to
privacy^
     Thank you  for  your cooperation.  Should you  have any questions
please call the study office, 'collect (612)  376-8775.

                                    Sincerely yours,
                                    Leonard M.  Schuman,  M.D.
                                    Professor  and  Director
LMS:kb
encl..
                             HEALTH SCIENCES

-------
          UNIVERSITY OF MINNESOTA
          TWIN CITIES
Division ot Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis, Minnesota 55455
Dear Fisherman:

     The sport and commercial fishing industries of  the Great
Lakes region are of significant economic importance  to the' Great.
Lakes states.  The health  and well-being of the members of the
fishing industry are  important to their families as  well as to
the economy of the Great Lakes Basin.
     In 1978 our "Great  Lakes Project" was initiated to evaluate
the health of those individuals involved wi'th  sport  or-commercial
fishing in the Great  Lakes.   It is our understanding that you have
either served on a commercial fishing vessel or have possessed a
commercial fishing license.
     Enclosed with this  letter is a set of questions regarding
your fishing practices and health status.  A medical consent form
is also enclosed.  In a  couple of days an interviewer will con-
tact you by phone and request your answers to  this set of -ques-
tions.  Your answers  will  be kept confidential.  This is a
medically-related survey and we uphold your right  to privacy.
     Thank you for your  cooperation.-  Should you have.any questions
please call the study office, collect (612) 376-8775;
                                    Sincetely yours,
                                    Leonard M.  Schuman,  M.D.
                                    Professor and  Director
LMS:kb
encl.
                             HEALTH SCIENCES

-------
         UNIVERSITY OF MINNESOTA
         TWIN CITIES
Division of Epidemiology
School of Public" Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
     The sport  and  commercial fishing industries  of  the Great
Lakes region are of significant economic importance  to the Great
Lakes states.   The  health and well-being of the members of the
fishing industry are important to their families  as  well as to
the economy of  the  Great Lakes Basin.
     In 1978 the University of Minnesota initiated  a "Great Lakes
Study" to evaluate  the health of those individuals  involved with
sport or commercial fishing in the Great Lakes.   It  is our under-
standing that Mr./Mrs.	      served  on a commercial
vessel or held  a commercial fishing license.
     Enclosed with  this letter is a set of questions regarding
the fishing practices and prior health status of  Mr./Mrs.
	.  In a couple of days an interviewer will con-
tact you by phone to ask you how you  believe Mr./Mrs.	
would have answered these questions.  Your answers  will be kept
confidential.   This is a medically-related survey and we uphold
your and Mr./Mrs.	's right to  privacy.
     Thank you  for  your cooperation.  Should you  have any questions
plesse call the study office, collect  (612) 376-8775.

                                    Sincerely yours,
                                    Leonard M.  Schuman, M.D,
                                    Professor  and Director
LMSrkb
end.
                            HEALTH SCIENCES

-------
          UNIVERSITY OF MINNESOTA
          TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
Dear Fisherman-..

     The sport and commercial  fishing industries of the Great
Lakes region are of significant  economic importance to the Great
Lakes states.  The health  and  well-being of the members of the
fishing industry are  important, to their families as well. as  to
the economy of the Great Lakes Basin.
     In 1978 our "Great Lakes  Project" was initiated to evaluate
the health of those individuals  involved with sport or commercial
fishing in the Great  Lakes.   It  is our understanding that you have
either served on a commercial  fishing vessel or have possessed  a
commercial fishing license.
     Enclosed with this letter is a questionnaire which asks
questions about your  fishing  practices and health -status .:  We
would like you to fill out the questionnaire to the best of .your
ability.  A section at the end of the questionnaire is important
for your wife to complete. Your answers will be kept confidential.
This is a medically-related survey and we uphold your right  to
privacy.
     On the last page you  will find a medical consent form.  We
ask you for your signature so as to permit us to ask your doctor (s)
for the exact diagnosis of the conditions you have listed.   If
your wife fills out the last  section of the questionnaire, we
ask that she sign the medical consent form as well.  Regardless
of whether you or your wife sign, complete the questionnaire and
mail it back in the prestamped self-addressed envelope provided.
     Thank you for your cooperation.  Should you have any questions
please call the study office,  collect  (612) 376-8775.

                                    Sincerely yours r
                                    Leonard M. Schuman, M.D.
                                    Professor and Director
LMS: te-b
encl.
                             HEALTH SCIENCES

-------
        UNIVERSITY OF MINNESOTA
        TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis, Minnesota 55455
     The sport  and  commercial fishing  industries of the Great
Lakes region  are  of significant economic  importance to the Great
LaKes states.   The  health and well-being  of  the  members of the
fishing industry  are important to their families as well as to
the economy of  the  Great Lakes Basin.
     In 1978  the  University of Minnesota  initiated a "Great Lakes
f'tudy" to evaluate  the health of those  individuals involved with
s,-;.->rt or commercial fishing in the Great  Lakes.   It is our under-
5-randing that Mr. /Mrs.           _ served  on a commercial fishing
                                  _
       or held  a  commercial fishing  license.
     Enclosed with this letter is a  questionnaire which asks
f.insstions regarding the prior fishing  practices and health status
01. Mr. /Mrs. _ ..  We ask  that  you  complete the question-
naire, as you think Mr. /Mrs.      _  would.  Your answers
will be kept confidential.   This is  a  medically-related survey and
we uphold your  right to privacy.
     Thank  you  for your cooperation.   Should  you have any questions
plaase call the study office, collect  (612)  376-8775.

                                    Sincerely  yours,
                                    Leonard  M.  Schuman,. M.D,
                                    Professor  and Director
LMS:kb
end.
                            HEALTH SCIENCES

-------
                       Great  Lakes  Study
                          Consent  form
Maae:
Current Street Address:
                         Street
                         City                     State         Zip
                         Phone


     I  {we) hereby give my  (our) permission  to .be  interviewed  by
the "Great Lakes Study" of  the University  of Minnesota.. -.My  (our)
involvement in this study is voluntary.  My  (our)  participation
consists of an interview of my  (our)  fishing practices and/or
health  status.
     I  (we) permit the  "Great Lakes  Study" to contact medical sources
listed  in the interview for the' purpose  of reviewing my  (our)
medical records.  I (we) understand  that all information  contained
within  the interview and the review  of medical records will  be
keat confidential and used  only for  research purposes.   I (we)
further understand that my  (our) names will  not  be associated  with
the results of this survey.
Husband                               Date
Wife                                  Date

-------
                        <3reat Lakes Study
                           Consent form
Name:
Current  Street  Address:
                         Street
                         City                       State       Zip
                         Phone
      I  (we)  hereby give my (our)  permission to be interviewed by
 the  "Great  Lakes  Study" of the  University of Minnesota.   My (our)
 involvement in  this study is  voluntary.   My (our) participation
 consisits of an interview of  my (our)  fishing practices  and/or
 health  status.
      I  (we)  understand that all information contained within the
 interview will  be kept confidential and  used only for research
 purposes.   I (we)  further understand that my (our)  names will not
 be associated with the results  of this survey.
.Husoahd                                   Date
 Wife                                       Date

-------
                        Great  Lakes  Study
                            Consent focm
Name:
Current Street Address:
                        Street
                        City                        State             Zip
                        Phone

     I hereby give my permission to be interviewed by the "Great Lakes
Study" of the University of Minnesota.  My involvement in this study is
voluntary.  My participation consists of an interview of the fishing
practices and prior health status of Mr./Mrs.	.	.
I understand that in answering questions about Mr./Mrs.	
I will report only that information which-I know to'be true, and'wiH not
be asked to make judgements or opinions about  the character of that in-
dividual.  I reserve the right tb refuse to answer any question regarding
Mr./Mrs.                	which I do not believe to be in their best
interest.

     I permit the'"Great Lakes Study" to contact the medical-sources
listed in the interview for the purpose of reviewing Mr./Mrs..	
medical records.  I understand that all information contained within the
interview and the review of medical records will be kept confidential
and used only for research purposes.   I further understand that..our names
will not be associated with the results of this survey.
Signature                                             Date
Relationship to Subject


Please return this form in the attached addressed, stamped envelope.
Thank you.

-------
                               Great  Lakes  Study
                               Draft:   Proxy Consent Form/ interview.only
Name:
Current Street Address:
                          Street
                         City                    State       Zip"
                         Phone


     I hereby give ray permission  to  be  interviewed by the  "Great
Lakes Study" of  the University of Minnesota.  My  involvement  in
this study is voluntary.  My participation  consists of an  inter-
view of the fishing practices and prior health status of Mr. /Mrs.
_ __.  I understand that  in  answering  questions  about
Mr. /Mrs. _     .     I will report  .only that  information  which
I know to be true and will not be asked to  make judgements  nor
opinions about the character of that individual.  I reserve the
right to refuse  to answer any question  regarding  Mr. /Mrs. _
which I do not believe  to be in their best- interest.
Signature                                 Date
Relat.ionshii) to deceased
Please return this  form  in  the  attached  addressed, stamped
envelope.  Thank you.

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           UNIVERSITY OF MINNESOTA
           TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E!
Minneapolis. Minnesota 55455
     Thank you for your participation in the health  study of
Great Lakes  commercial fishermen  conducted by the University
of Minnesota.   We appreciate your  contribution to this  research
in the field of preventative medicine.
     We are  writing to-request .your  signed consent form.
(Please find enclosed an .additional  consent form copy.if  you
have discarded the original.)  We  ask that you sign; >he.-consent
form and  return it in the addressed,  stamped envelope.  Please
be assured that all information we receive will be. kept- in .the.
strictest confidence.  Your  name .will not be associated with
the study results in any way.
     Thank you for your cooperation.

                                          Sincerely,
                                          Leonard M.  Schuman,,M.D.
                                          Professor and  Director
LMSrkb
encl.
                              HEALTH SCIENCES

-------
         UNIVERSITY OF MINNESOTA
         TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
                               REP:
     The patient named above is participating in a health study
of Great Lakes  commercial fishermen conducted by the University
of Minnesota.   This patient has given  us  permission to contact
you about his/her medical history.  A  copy pf his/her authoriza-
tion is enclosed.      .      .
     In order to classify this patient appropriately, we need
to verify patient conditions with the  exact diagnoses.  We would
appreciate your assistance in completing  the enclosed Medical
Records Abstract form.  If you would prefer to send copies of
clinical summaries or medical records,  please do so.
     We greatly appreciate your time and  effort in complying
with our request.  Should you have any questions, please call,
collect  (612) 376-8775.
     Thank you  for your  cooperation.

                                    Sincerely yours,
                                    Leonard  M.  Schuman, M.D.
                                    Professor and Director
LMSrkb
encl.
                             HEALTH SCIENCES

-------
         UNIVERSITY OF MINNESOTA
         TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis, Minnesota 55455
ATTN:  Medical  Records
                               REFr
Dear Madam/Sir:

     The patient named above is'participating "in a health  study
of.. Great Lakes  commercial fishermen  conducted by the University
of Minnesota.   This patient has given  us permission to contact
you about  his/her .medical history. ...A  copy of, his/her authoriza-
tion is enclosed.
     In order  to classify this patient properly,'we-need diag-
nostic information regarding past  and  present medical.conditions.
and would  appreciate your completing the enclosed .medical .records
abstract form,   if you would prefer  to send us a summary or  copy.
of these medical records-, please do  so.
     We wish to thank you in advance, for your time and effort in
complying  with  our request.  Should  you have any questions please
call the study  office, collect  (612) 376-8775.

                                    Sincerely yours,
                                     Leonard M. Schuman,  M.D,
                                     Professor and Director
 LMS:kb
 encl.
                             HEALTH SCIENCES

-------
          UNIVERSITY OF MINNESOTA
          TWIN CITIES
Division of Epidemiology
School of Public-Health
1360 Mayo Memorial Building
420 Dataware Street S.E.
Minneapolis, Minnesota 55455
ATTN:  Medical  Records
                               REF:
Dear Madam/Sir:

     The  individual named above has  indicated that he/she, was a
patient in  your  hospital.  This individual  is participating in
a health  study of Great Lakes commercial  fishermen conducted by
the University of Minnesota and has  given us  permission to review
his/her medical  records.   A copy of  his/her consent form is en-
closed.
     In order to classify this individual appropriately, we need
to verify the patient's hospitalizations  and  diagnoses at your
institution.  We would appreciate your  assistance in completing
the enclosed medical records abstract.  If  you prefer to send
copies of clinical summaries or medical records,  please do so.
     We greatly  appreciate your time and  effort in complying
with our  request.  Should you have any  questions, please call
the study office, collect (612)376-8775.
     Thank  you for your cooperation.

                                    Sincerely  yours,
                                    Leonard  M.  Schuman, M.D.
                                    Professor  and Director
LMSrkb
encl.
                             HEALTH SCIENCES

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          UNIVERSITY OF MINNESOTA
          TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
                               REF:
     Several weeks  ago we requested some  specific medical infor-
mation regarding  one of your patients.  In  the  event that you did
not receive that  communication, or misplaced  the original copy,
we enclose an  additional form for your convenience.       .    . .
     The patient  named above 'is participating in a health study
of Great Lakes commercial- fishermen conducted by the University
of Minnesota and  has given us permission  to contact you regarding
his/her medical history.  A copy of his/her authorization is
enclosed.
     In order  to' classify this patient appropriately/, we .need to
verify patient responses with exact medical records.  We would
appreciate your assistance by filling out the attached form..... If
you.would prefer  to send clinical summaries or  copies--of-records,
please do so.
     We appreciate  your time and effort in  complying with..our
request.  If you  have already returned the  form, please disregard
this letter.   Should you have any questions,  do not hesitate'to'
.call the study office, collect (612) 376-8775.
     Thank you for  your cooperation.

                                    Sincerely  yours,
                                    Leonard  M.  Schuman, M.D.
                                    Professor and Director
LMSrkb
encl.
                             HEALTH SCIENCES ,

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                      Great Lakes Study
                    Medical Records Abstract
Dr./Mr./Mrs.
               has authorized the
University of Minnesota, Department of Epidemiology, to review
their medical records.  A copy of the signed consent form is
attached.  We ask for your cooperation in verifying diagnoses
made by yourself or your staff in the study participant.

     (Obstetricians and Gynecologists please skip the first two
questions and answer questions three, four and five).  Thank you
for your cooperation.  •
1.  If you or any staff members have confirmed or diagnosed any
    of the following conditions, please check the corresponding
    box and write the exact date of diagnosis.
Disease Name
Condition has been confirmed     Date of.
or diagnosed in subject (•}      diagnosis
Asthma
Bronchitis
Emphysema
Tuberculosis -TB
Mcnonucleosis - Mono
Pneumonia
Any other respiratory
disease
SPECIFY:
Hepatitis or yellow
jaundice
Cirrhosis
Any other liver
disease
SPECIFY:
Spondylitis
Gout
Rheumatoid arthritis
Osteoarthritis
Any other diseases of
bones and joints
SPECIFY:
High Triglycerides
Meningitis
Hypertension























-













-------
Condition has been confirmed Date of
Disease Name or diagnosed in subject (V) Diagnosis

High Cholesterol
Angina pectoris
Heart attack or MI
Stroke- cerebral accident
or CVA- hemorrhage,
thrombosis, emoblism
Any other heart, or
circulatory diseases
SPECIFY:
Diabetes
Thyroiditis
Any other glandular
disorders
SPECIFY:
Eye diseases
SPECIFY:
Multiple Sclerosis-MS
Any other disease of
the nervous system
SPECIFY:
Anemia
Gastritis
Ulcers (stomach or
duodenal)
Any other disease of
the digestive system
SPECIFIC:'
Psoriasis
Eczema
Herpes Zoster (dermatitis)
Shingles
Pemphigus
Any other skin disease
SPECIFY:
Cancers:
Breast
Stomach
Esophagus
Mouth or Tongue
Large intestine
Rectum
Trachea, Bronchus
or Lung































--























-------
Disease Name
Condition has-been confirmed   Date o'f.
or diagnosed in subject ft/}    Diagnosis
Cancers :
Liver or Biliary passage
Bladder and Urinary organs
Skin
Thyroid
Leukemia
Other:
Mental Retardation














2.  If you or any staff members have been consulted  regarding any
    of the following symptoms, please check the corresponding box
    and write in the exact date(s) of consultation.
Condition
       Condition has been     Date of
       confirmed in subject   Diagnosis
Severe fever
Extreme tiredness.
Frequent or very bad headaches
Problems with mouth or throat
Any problems with ears or hearing
SPECIFY:
«ny unusual difficulty with eyes or
eyesight other than a change of the
prescription of glasses
SPECIFY":
Sudden weakness or heaviness of
arms or legs
SPECIFY:
Numbness in arms or legs
Swelling in arms 'or legs
Stiffness in joints or bones
SPECIFY:
Pains in joints or bones
SPECIFY:
Spasms- of- limbs
Spells of dizziness
Fainted or blacked out
Very strong heartbeats
Irregular (fast, slow, or incon-
stant) heartbeats
Pain, discomfort, or trouble in
or around vour heart
Itching of the skin

















L . : . 	 	



















-------
 Condition^
                         Condition has been
                         confirmed in subject
                                                     Date of
                                                     Diacnosis
Any unusual discoloration or
erruptions on the skin
SPECIFY:
Any problems with your stomach
or digestive system
SPECIFY:
Swollen glands in neck, armpits
or groin






 3.  Please list all the pregnancies and outcomes which you or your
     staff members provided medical care and assistance.
Pregnancy
Number
1
2
3
Preznancv Outcome
Abortion Miscarriage Stillbirth Live Birth



' I
5
6




















Infants
Sex
M F












Date. of Birth






Live ,
Birth '
Weight






 4,  Has the subject ever consulted you or your:staff members
     regarding abnormal spotting or bleeding between her menstrual
     cycles?
O
No
             Yes
                             Diagnosis;

                             Date :
     Has the subject ever had surgery on her reproductive organs
     performed by you or your staff members?
    No
             Yes
                             Diagnosis:

                             Date:
                             Type of surgery:^

                             Date of surgery:
6.   Has the subject experienced complications in &tiy of the above
    listed pregnancies?
       O
No
               Yes
                 Diagnosis':

                 Date:
                             Type of treatments^

                             Etiology;

-------
CONSENT FOHM
CURRENT ADDRESS*
                         HUOAUUINU
PHONE (I!) :
      <0)J
INTERV1EWERS_

NEVER REC'D:_
UPDATE:
                                                                                     OTHERt
(1) CONTACT (Physician,
Clinic or Hospital) t



DATE & REASON FOR CONTACT
REGARD ING i
Mr/Mrs

OTHER INFOR!-SATIOt4£

(1) CONTACT (Physician,
Clinic or Hospital) t



DATE & REASON FOR CONTACT
REGARDING}
Mr/Mrs

OTHER I N FORM AT I ON i

oATfi itfc.rriiRS.SGNT to MEDICAL SOURCES! RETURNS FROM MEDICAL SOURCES j
1st
Mailing


2nJ mailing
6 phone call


Comments from
Phone calls


Abstract
Forms


No
Records


Chart
Copy


Other



-------
          UNIVERSITY OF MINNESOTA
          TWIN CITIES
Division of Epidemiology
School of Public Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis. Minnesota 55455
Dear Sir:

     The Departments  of Environmental Health  and Epidemiology,
University of Minnesota, are currently involved in a s-tudy-to
evaluate the health status of commercial  fishermen in the Great
Lakes Basin.  The  validation procedure of this  study involves
the collection  of  medical records for living  cohort members and
the collection  of  death certificates for  deceased cohort.members.
     We would appreciate receiving one-copy of  the death-certi-
ficate of the person(s) .listed on the attached  sheet. .We are
listing.the full name,  social security number,  date of birth, and.
date of death to-assist in matching.  In  addition, we have de-
veloped a coding system for the results of your search (see
attached sheet).   Please return the roster with the death certi-
ficate and your notations.
     Enclosed you  will find a check for the retrieval costs of
the death certificate search.  Thank you  for  your cooperation.

                                    Sincerely  yours,
                                    Leonard  M.  Schuman, M.D.
                                    Professor and Director
LMS:kb
end.
                             HEALTH SCIENCES

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                   UNIVERSITY OF MINNESOTA
                   TWIN CITIES
                                                                          Page 2
                Division of Epidemiology
                School of PubltcTHealth
                1360 Mayo Memorial Building
                420 Delaware Street S.E.
                Minneapolis, Minnesota 55455
                        Commercial Fishermen  Death Certificates
No.   Name
Death  Date    Birth Date  Social Security Number  Results
*Code
  + =  found
  - =  not found
  I =  further  search  needed
  C =  comment  (add  in space  below name)
                                        HEALTH SCIENCES

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           UNIVERSITY OF MINNESOTA
           TWIN CITIES
Division of Epidemiology
SchooJ of Public-Health
1360 Mayo Memorial Building
420 Delaware Street S.E.
Minneapolis, Minnesota 55455
     The Division of Epidemiology of  the  University of Minnesota
is conducting  a  study to determine  the  general health" patterns, of
families involved in the commercial fishing industry.
     We have tried to contact you by  phone to ask a few'simple
questions about  you and your family's health. -Since we have
been unable to reach you, we ask that you please call us •collect
at:.  (612) 376-3775.  Or, please write  on the enclosed, card a
phone number where we can reach you.
     Your contribution is important to  the success of this
project.  We are willing to talk with you at a time that is
convenient for you.  All information  that you share 'with us•
will be confidential.  Your name will not be associated with
the study results.
     We would  be glad to answer any questions that you may have
regarding this study.
     Thank you for your cooperation.

                                         Sincerely,
                                          Leonard M. Schuman, M.D.
                                          Professor and Director
                               HEALTH SCIENCES

-------
NAME:  	

      My phone number is  (
      I prefer not to release my phone
      number, but I will call the study
      office, collect {612} 376-8775 on
      	    	at	.
            (date)             (time)


Thank you for your cooperation in this study.

-------
     We have provided the following list of questions for your  review
prior to the interviewer's call.  We suggest that as you read through
this list you note all necessary names, dates, and addresses requested,
We hope that in providing you with an advanced -listing of the inter-
view questions, you will have time to consider your answers and,gather
information needed.  Thank you for your cooperation.

What is your current address?
What is your sex?
What is your race?
What is your birthdate?
What is your Social Security number?
What is your marital status?
Do you presently hold a commercial fishing  license?
Oid you hold a commercial fishing license  in  the  past?
     Approximately over what years have you held  a  license?
     Have you' ever .been a crew member  for,  or  a partner  with,;an
     individual owning a commercial fishing license?
     Over what years have you been a crew member  or par.tner?
Is commercial fishing your current full-time  occupation?
     Over what years have you commercially  fished full-time?
Do you currently own a sport fishing license?
To the best of your knowledge, please  tell  me .the names  and addresses
of full or part-time crew members?   (past and  present)
Do  (or did) you consume any of the fish you catch commercially  or. as
a sport fisherman?
Approximately how -many of your meals contain  fish caught (by yourself
or a friend) from  the Great Lakes?
Approximately how  many years have, you  consumed Great  Lakes'-fish with
this frequency?
Approximately how  many of your meals contain  fish caught (by yourself
or a friend) from  waters other than the Great Lakes?
How often do your  family members eat fish?
Approximately how  many pounds of fish  have  you consumed  per year?
      (assume:  1  fish meal equals % pound  of  fish)
     What cooking  methods are used in  preparing the  fish you consume?
Do you presently  smoke cigarettes?
     How old were  you when you  first began  to smoke  cigarettes?
     What  is the  average number of cigarettes you presently smoke
     per day?
     How many years have you smoked,cigarettes with  this.frequency?

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page 2

Did you smoke cigarettes in the past?
     How old were you when you first began to smoke cigarettes?
     How many years did you smoke cigarettes?
Do you presently smoke a. pipe?
     How old were you when you first began to smoke a pipe?
     What is the average number of pipefuls you presently smoke per day?
     How many years have you smoked a pipe with this frequency?
Did you smoke a pipe in the past?
     How old were you when you first began to smoke a pipe?
     How many years did you smoke a pipe?
Do you presently smoke cigars?
     How old were you when you began to smoke cigars?
     What is the average number of cigars you presently smoke per day?
     How many years have you smoked cigars with this frequency?
Did you smoke cigars in the past?
     How old were you when you began to smoke cigars?
     How many years did you smoke cigars?
Do you drink hard liquors?
     How old were you when you first began to drink hard liquor?
     What is the average number of hard liquor drinks you.presently
     consume per week?
     How many years have you consumed h'ard liquor with this frequency?
Did you consume hard liquor in the past?
     How old were you when you first began to drink hard liquor?
     How many years did you consume hard liquor drinks?
Do you drink beer?
     How old were you when you first began to drink beer?
     What is the average number of beers  (12 02. bottles) you presently
     consume per week?
     How many years have you consumed beer with this frequency?
Did you drink beer in the past?
     How old were you when you first began to drink beer?
     How many years did you consume beer?
Do you drink wine?
     How old were you when you first began to drink wine?
     What is the average number of wine drinks you have per ,week?
     How maay years have you consumed wine with this frequency?
Did you drink wine in the past?
     How old were you when you frist began to drink wine?
     How many years did you consume wine?

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page 3
Have you, your wife, or any of your children ever had  any  of  the
following diseases or conditions.
Asthma
Bronchitis
Emphys ema
Tuberculosis-TB
Mononucleosis-Mono-
  Kissing Desease
Pneumonia
Any other disease of
  respiratory system
  Specify:
Hepatitis or yellow
  jaundice
Cirrhosis
Any other liver disease
  Specify:	__
Spondylitis
Gout
Rheumatoid Arthritis
Osteoarthritis
Any other diseases of bones
  or joints
  Specify:	_«_^__
High tr iglycerides
Meningitis
Hypertension or high blood
  pressure
High cholesterol
Angina pectoris
Heart .attack-Ml or coronary
Stroke-cerebral accident-(CVA)
Any. other diseases of the heart
  or circulatory system in the
  family
  Specify:	._..__
.Diabetes
Thyroiditis
Any other glandular disorder
  Specify:
 For each disease listed above  you  will  be  asked to  list  the  names  of
 family members who  have had  the  disease, the  consulting  doctor  or
 clinic, and .the date of diagnosis.
Eye diseases
  Specify:	
Psoriasis
Eczema
Herpes zoster-shingles .-(dermatitis)
Pemphigus
Any other diseases of skin
  Specify:	
Anemia
Gastritis
Ulcers  (stomach or duodenal)
Any other diseases of the "digestive-
  system
  Specif y-:_	
Cancer of the breast
Cancer of the stomach
Cancer of the esophagus
Cancer of the mouth or tongue
Cancer of the large intestine
Cancer, of the rectum
Cancer of the trachea., bronchus,  lung
Cancer of the liver or biliary  passage
Cancer of the bladde-r o-r urinary
  organs
Cancer of the skin
Cancer of the thyroid
leukemia
Other cancer
  Specify;_	
Mental Retardation
 How  many  natural  and  step  or  adopted  children do  you have?

 Please  list  all pregnancies and  outcomes  of  which you were  the father/
 mother  in the  sequence  .they occurred.   For each pregnancy we will ask:
      Were there complications?

      Was  the outcome  a  miscarriage, stillbir.th," live birth, .or other?
      What was  the child's  sex?

      What was  the date  of. birth?

      What was  tfie live  birth  -weight?

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     Name the hospital, clinic, or place of birth.

     What was the doctors name?

Have any of your natural children died?  If yes, what was the child'-s
sex, date of birth, date of death, cause of death, and the city and
state in which the death was reported?

Do you know of any mental or physical abnormalities at birth  (birth
defects) in yourself or your natural children.  If yes, who had the
defect, are they living or dead, and what  type of defect did they have?

Have you ever had any of the following symptoms?
  Severe fever                        Have you had spells of dizziness?
  Extreme tiredness                   Have you fainted or blacked out?
  Frequent or very bad headaches      Very strong heartbeats?
  Problems with mouth or throat       Irregular (fast, slow, or inconstant)
  Any prodlems with ears or hearing     heartbeats?
    Specify:          	_,«___    Have you had pain, discomfort, or
  Any unusual difficulty "with eyes      trouble in or around your ..heart?
    or eyesight other than a change   Itching of the skin?
    of the prescription of glasses?   Any  unusual discoloration or erup-
    Specify:	  ^__            tions on the skin?
  Sudden weakness or heaviness of       Specify;     	,
    arms or legs?                     Any  problems with your stomach or
    Specify;	•__		  	.__       digestive system?
  Numbness in "arms" or legs ?Specify:	.
  Swelling in arms or legs?           Swollen glands in your neck, armpits
  Stiffness in joints or bones?         or groin?
    Specify:            		     Have you lost 20 or more pounds in
  Pains in joints or bones?"~"       the last five years?  (include
    Specify;	  _ _	;	'_,         dieting)
  Spasms of limbs1?  '  *     " '   ~


If you have experienced any of the above symptoms you will be asked
how frequently they occured, how long the  symptoms lasted, the year
you most recently experienced the symptoms, and the names of the doctors
you consulted if applicable.
Fisherwomen  or  the  wives  of male fishermen will  be  asked  the  fol-
lowing  questions.   If the wife is deceased we  will  ask  that the
husband answer  these questions to the best of  his knowledge.
Do you  use a birth  control  method?

What method of  birth  control do  you  use?

At what  age did you begin using  birth control  methods?

At what  age did you begin to menstruate?

Have you stopped menstruating?

At what  age did you stop?

Did the  cessation occur naturally or due  to surgery?  If  due  to surqery,
what was the reason for the surgery  and what was the doctor's name?

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pa-ge 5
What is  (was) the average  length of  your  menstrual cycle? (Frpm frrs-t
day of bleeding to first day of next period)

On the average, how long is  (was) your  period?

In the past five years, has your blood  flow during menstruation increased,
decreased, or stayed the same?

Have (or did) you experience any abnormal spotting1 or .bleeding be.tweten'
your menstrual cycles?  If yes, you  will  be asked to give the. nawe of
the doctor you consulted if applicable.

Have; you used, or did you  use, any medications  prescribed .by -a doc.to.r'
for menstrual irregularities?  If yes,  what was the name of the medication,

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                                GREAT  LAKES FISHERMEN HEALTH STUDY
                                   Respondent Information  Sheet
Respondent ID Number:    I  I  I  I
Name:
       last                 first                middle
Address:
         street
         city                             state         zip
Telephone:
           area code  number

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                 GREAT LAKES FISHERMEN HEALTH STUDY
                   INTERVIEWER ASSIGNMENT RECORD
Interviewer Name:



Interviewer
Assignments -Interviews Gormen ts
Resoondent ID#
















Date Assigned
















Date of
Interview















!
Date of
Return
















Refusal, Unavailable., etc.

















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                                 DEFINITIONS
62.  Asthma


63.  Bronchitis


64.  Emphysema



65.  Tuberculosis-IB

66.  Mononucleosis-Mono-
     Kissing Disease



67.  Pneumonia

69.  Hepatitis or yellow
     jaundice

70.  Cirrhosis of the liver


72.  Sponcylitis

73.  Gout


74.  Rheumatoid Arthritis


75.  Osteoarthritis


77.  Meningitis


78.  Hypertension or high
     blood pressure

79.  High cholesterol

80.  Angina pectoris
81.  Heart attack-Mi or
     coronary
A condition marked by"recurrent attacks of breathing
difficulties marked by-wheezing

Inflammation and usually infection of the bronchi,
which is a part of the lung

A lung disease characterized by destruction of lung
tissue causing difficulty in breathing, often secondary
to smoking

A specific infectious disease usually affecting the lung.
A virus disease characterized by severe changes in
the white blood cells causing fatigue, severe sore
throat, and swollen lymph glands

Infection of the lungs
Inflammation of the liver, frequently infectious

A chronic disease of the liver characterized by the
replacement of normal tissue by scar tissue

Inflammation of the spine

A genetic form.of arthritis which affects chiefly men
and is due to high levels of uric acid in the blood

A persistent disease of the joints characterized by
deformity and pain in the joints

A degenerative joint disease associated with wear and
tear of the tissues and with aging

Inflammation of the membrane around the brain and spinal
cord usually caused by infection
Persistently high blood pressure

A high level of cholesterol in the blood

Sudden tightness and pain in the chest occuring during
physical exertion or tension, and subsiding with rest,
caused by disease in the coronary arteries


Death of any muscle tissue of the heart

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 82.   Stroke,  cerebral accident,
      (CVA)-hemorrhage, thrombosis,
      embolism



 84.   Diabetes


 85..  Thyroiditis

 88.   Psoriasis


 39.   Eczema

 90.   Herpes zoster-shingles
      (dermatitis)



 91.   Pemphigus


 93.   Multiple Sclerosis



 95.   Anemia


 9.6.   Gastritis

 97.   Ulcers (stomach or
      duodenal)
Death of brain tissue which results from lack of blood
to a portion of the brain or from a hemorrhage in the
brain

A disease which is characterized-by-high levels of
sugar in the blood

Inflammation of the thyroid gland

A chronic disease of the skin which usually involves
the scalp, elbows, knees, and shins

An inflammation, generally of the skin
An inflammatory disease of nerves caused 'by the virus
of chicken pox and characterized by groups of ..small.
blisters in the skin
A disease characterized by clusters of large
blisters
A disease of the central nervous system.  Some symptoms
are lack of coordination, weakness, speech and visual
problems

A condition in which there are low levels of red blood
cells in the body

Persistent inflammation of the stomach
A local cavity in the inside surface of the stomach or
duodenum which usually results-from..-inflammation in
that area
100.  Leukemia
101.  Hodgkin's Disease
A chronic disease characterized by an abnormal, increase.
in the number of leukocytes in the tissues and often- in
the blood

A disease marked by chronic enlargement of the lymph
nodes, often cervical at the onset and then generalized,
together with enlargement of the spleen and often  of the
liver

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          GREAT LAKES FISHERMEN HEALTH SURVEY
                   TABLE OF OCNTEMS
!•  Intrtjduction to Surveys

     What is a Survey	1
     Interviewing	2
     Ethics of Survey Interviewing	...2
2-  Using the Questionnaire

    Asking the Questions	4
    Maintaining Rapport	«	5
    Probing	7
    Kinds of Probes	-	8
    The Don't Know Response	10

3.  Question by Question Specifications.	13
4.  Respondent Letter	21
5.  Consent Form	22

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                    INTRODUCTION TO SURVEYS

What is a Survey?

     A survey usually involves collecting data from a group
of people selected to accurately represent the population
under study.  This group of people is called a sample.  People
 in the  sample  are  asked a  series of  questions  through the
use of a questionnaire.  The answers obtained are put together
in an organized manner so that conclusions can be drawn.  This
information is then used in planning, research, and solving
particular problems.
     Skillful interviewing procedures are used to ensure full
and accurate information.  Careful methods are followed so
that the data gathered from the sample or respondents can
be confidently used to represent the total population.  The
use of the sample means that a small number of•respondents
can be selected.to represent the whole population, making
it possible to avoid the expensive and time consuming' proc-
edure of taking a census {a census involves a complete ac-
counting of every person in the population being studied).

Interviewing

     The interviewing stage, which is one of the core opera-
tions of any survey, includes the.recruitment and training

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of interviewers, the preparation of general and specific
interviewing instructions,field supervision, administration
of interviews, and the validation of interviews.
     During the interviewing period, the success of the survey
rests solely in the hands of the interviewer.  Researchers
strive to develop the best interview guides possible, but
even the best interview guide is only as good as the inter-
viewer's skill in using it.

Ethics of Survey Interviewing

     Persons working in jobs and professions which deal
with the experiences, thoughts, actions and feelings of
people have an ethical responsibility to these people.  Sur-
vey research interviewing is one of these occupations, and
interviewers roust, therefore, accept the ethics of the
profession.  Just as doctors and lawyers must respect in-
formation about their patients and clients as privileged,
so must the survey interviewer.
     The interviewer must often ask questions that one
would not think of asking a close friend; questions which
might be thought of as "too personal."  You will find that
the average person is willing to answer these questions,
sometimes offering information which would not .be given even
to a close friend or relative.  Your protection of all infor-
mation about respondents gained during the conduct of re-

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search is therefore essential.



     The main reason research studies can point to success



is confidentiality. Interviewers can, and do, promise the



people who are interviewed that their answers to the., ques-



tions will be kept strictly confidential.

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                    USING THE QUESTIONNAIRE









Asking the Questions








     The interviewer's goal is to collect accurate information



through the use of the study questionnaire.  Data from study



participants must be collected in a uniform manner.  Thus,



all people in a sample must be asked the same questions in



the same way.



     The following principles and techniques must be em-



ployed when using the questionnaire:



     1.  Always remain neutral. The interviewer must be



     careful that nothing in words or manner implies crit-



     icism, surprise, approval, or disapproval of either



     the questions asked or the respondent's answers.



     Through a relaxed professional attitude, the inter-



     viewer can put the respondent at ease and gain his



     confidence.  We need the respondent's answers to the



     questions with as little influence as possible by the



     interviewer.  Another interviewer should be able to



     obtain the same answers.



     2.  Ask ALL questions in the order presented in the



     questionnaire.  Never change the order of the questions



     in the questionnaire.  The questions follow one an-



     other in a logical sequence; to change that sequence



     would subvert the intent of the questionnaire.

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     3.  Ask ALL questions exactly as worded. Do not change
     even one word in the question that is printed for you.
     Many times, the smallest change can affect the whole
     meaning of the question.  Simply repeat the question
     if the need arises.  If you do repeat the question,.
     read all the words in the question.  Even though you
     feel that a question could be worded much more simply,
     do not improvise on the method of asking the question.
     Every word is there for a purpose.  In order for all the
     interviewer's work to be combined there must be.no
     doubt that each respondent heard exactly the same ques-
     tion before answering.


Maintaining Rapport

     You began your rapport-building process'with your
introduction and it must be continued throughout the
interview.  Through your accepting and understanding
behavior and your interest in the respondent,, you can
create a friendly atmosphere in which the respondent can
talk freely and fully.
     Occasionally, however, rapport may be broken during
the interview because the respondent finds a particular
question "too personal" for example.   If the respondent
feels a question is- too personal, take time to reassure him
that he may speak freely without fear..  This may be done by

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restating the confidential nature of the questionnaire and



the anonymous nature of the study.  If a respondent refuses



to answer a question after you have reassured him of



confidentiality, do not press him.  Record what the



respondent said in refusing to answer the question and



proceed to the next question.  The interviewer should not



irritate the respondent and provoke a refusal to complete



the interview.

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Probing
     Probing is the technique used by the interviewer to
stimulate discussion and obtain more information.  -A ques-
tion has been asked and an answer is given.  For any number
of reasons, the answer may be inadequate, requiring the inter-
viewer to seek more information.  Probing, therefore, has
three major functions:  (1) to motivate the respondent to
enlarge, clarify, or explain his answers;  (2) to focus the
respondent's answers so that irrelevant and unnecessary
information can be eliminated; and  (3) to pinpoint objective
information, such as dates" and names, as accurately as pos-
sible.  This must be done, however, without introducing
bias or antagonizing the respondent.
     You must fully understand the purpose and meaning of
each question. Once you understand the purpose of a question,
•you will find it much easier to decide if you have a satis-
factory answer or whether you should probe for a clearer and
more complete one.
     Probes must alway remain neutral. Remember, probing is
to motivate the respondent to respond more fully -or to focus
his answer without introducing bias.  The potential for bias
is great in the use of probes.  Under the pressure of the
interviewing situation, the interviewer may..quite uninten-
tionally imply that some answers are more acceptable than
others or may hint that a respondent might want to consider

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this or include that in giving a response.







Kinds of Probes








     A nun±>er of different neutral probes which appear as



part of a normal conversation can be used to stimulate a



fuller, complete response.





1. An expression of interest and understanding. By comments



such as "uh-huh" or "I see" or "yes", the interviewer in-



dicates that the response has been heard, that it is interes-



ting, and that more is expected.



2. An expectant pause. The simplest way to convey to the re-



spondent that you know he has begun to answer the question,



but has more to say, is to be silent.  The pause	often



accompanied'by an expectant look or nod of the head	al-



lows the respondent time to gather his thoughts.



3. Repeat the question. When the respondent does not seem



to understand the question, misinterprets it, seems unable



to decide, or strays from the subject, it is often useful



to repeat the question.  Many respondents, when hearing the



question a second time, realize what kind of answer is needed.



4. Repeating the respondent's reply. Simply repeating what



the respondent has said is often an excellent probe.  Hearing



the response just given often stimulates the respondent to



further thought.



5. A neutral question or comment. Neutral questions or -com-

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ments are often used to obtain clearer and.fuller  responses



Following are some suggestions for probing  "techniques.





Probes to Clarify








What do you mean exactly?



What do you mean by	?



Could you please explain that a little?



I don't think I quite understand.







Probes for specificity








What in particular do you have in mind?



Could you be more specific about that?



Tell me about that.  .What/who/how/why/when....?








Probes for data specificity







Was tha-t before or after your first hospitaliza.t.ion?



Were you married during that' time?



Who were you working for at the time?







Probes for relevance







I see.  Well, let me ask you again..REPEAT  EXACT QUESTION.



Would you tell me exactly how you mean that?

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                            10
Probes for completeness

What else?
What else can you think of?
What other reason/things/examples, ect. can you think.of?

The Don't Know Response

     The "I don't know" answer can mean a number of things.
For instance:
     1. The respondent doesn't understand the question and
        says 1 don't know to avoid saying he doesn't under-
        stand.
     2. The respondent is thinking the question over, and
        says I don't know to fill the silence and give him-
        self time to think.
     3. The respondent may be trying to evade the issue be-
        cause he feels uninformed, or is afraid of giving
        the wrong answer, or because the question seems too
        personal.
     4. The respondent may really not know the answer to
        a question.
.If the respondent actually doesn't have the information re-
quested of him, this in itself is significant to the study
results.  It is the interviewer's responsibility, however,
to make certain this is the case.  An expectant pause; a

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                            11
reassuring remark, repeating the question, a neutral question-
all will encourage the respondent to reply.

Examples of Probing

     The following are illustrations of probing which will
help you avoid biasing the respondent's answer:

1. Don't ask whether a person means "this or that."  This
   suggests only one of two answers, even though there may.
   be many other possibilities which the respondent is think-.
   ing about.

   QUESTION; At the present time, what is the average number
             of cigarettes you smoke per day?
   RESPONSE; Oh, just a few.
   IMPROPER PROBE; Would that be three or four?
   (You are pushing the respondent to one of two alternatives
   when he might mean something else entirely)
   PROPER PROBE; And how many cigarettes is that?

2- Don't ask whether the respondent meant a particular thing
   by a certain word.  This suggests one answer, when he
   might actually have another one in mind.

   QUESTION:'What is your .occupation at the present time?
   RESPONSE: I assist the manager.

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                        12
IMPROPER PROBE; Oh, you're a supervisor?
(The incorrect probe is an attempt to define for the re-
spondent.  A neutral probe will give the respondent an
opportunity to tell what is meant.)
PROPER PROBE; Assisting the manager?  Could you tell me
a little bit more about that?

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                            13
           QUESTION BY QUESTION SPECIFICATIONS

     Throughout the questionnaire, there are interviewer
instructions and parts of some questions which are not
meant to be read to the respondent (R).  Those instruc-
tions and responses which should not be read are
CAPITALIZED.  The numbered responses in mpsjt questions
should not be read to the R.   Follow the interviewer's
instructions carefully and record all responses clearly
and legibly.
     During an interview, a R may answer "I don't know,"
or he may refuse to answer a question.  In this case, these
responses should be recorded as "don't know" or "refuse to
answer" respectively.

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                                14
                 QUESTION BY QUESTION SPEOFICATIONS
Cl      Verify address with respondent.

Q2      Record month, day and year of birth.  Do not accept "I can't
        remember* as an answer.  If R seems uncertain about his date of birth,
        suggest that he look at his driver's license.  Write out the month;
        do not use a numerical value.

Q3      If R responds "single" probe to determine whether he has ever been
        married.  "Single" may only be a response if R has never been married.

Q4      Record number of children.  If R has no children record "0" in the
        blank.  If R offers additional information regarding step and/or adopted
        children, note this in the white space below.

Q5      If no, go to Q6.  If yes, record the number of years R has held a
        oormercial fishing license.  Next, obtain the span of years involved
         (i.e. "from 1959 to 1979") and probe for different periods of tiros.
        For example, if R has held a carmsrcial fishing license for 20 years
        we need to know if he held that license for a consecutive 20 year period
        or if instead, he has held a license on and off for a total of 20 years
         (i.e.- fron 1950 to 1960; from 1965 to 1970, and from 1974 to 1979).

C6s7    Same specifications as Q5

        I? R ANSWERS NO TO Q5, 6, and 7, STOP THE INTERVIEW.  TRY TO DETERMINE
        IF WE HAVE THE CORRECT RESPONDENT.  THANK HIM FOR HIS TIME AND COOPERATION
        AND EXPLAIN THAT CURRENTLY WE ARE ONLY INTERVIEWING PEOPLE WHO HAVE
        COMMERCIALLY FISHED.

Q8      Ask as written.  It is especially important to obtain the state where
        these towns are located.

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                               15
Q8a     Ask as written.  A hailing port is where the fisherman decks his
        boat.

Q9      If no, go to Q10.  If yes, record the number of years cotmercial
        fishing has been his full time occupation.  Next, obtain the span
        of years involved  (i.e. "from 1969 to 1979") and probe for' different
        periods of time.  For example, if R's full time cccuapticn was
        corinercial fishing for 10 years, we need to know if this was a
        consecutive 10 year period of if instead, he has been a full time
        fisherman on and off for a total_of 10 years  (i.e. from 1959 -to 1961;...
        from 1971 to 1979).

Q10     Probe for specifics. If R holds a job which does not have a specific
        job title, ask for a description of the' type of work.  Probe to obtain
        a job title which reflects the type of work performed-as accurately
        as possible.  As a rule, one-word entries are usually inadequate.   For
        example:

                Inad£~iate                        Specific

                Factory worker                    Electric motor assembler,
                                                  forge heater, punch press
                                                  operator, spray painter,  turret
                                                  lathe operator
                Labortir                           Sweeper, porter, janitor, window
                                                  washer, hand trucker, stevedore
                Foreman                           Specify the' craft or activity
                                                  irtvolved such as foreman  carpente:
                                                  foreman truck driver,, etc.

Qll     Same specifications as Q9.

Q12     Correct spelling and address information are  imperative. Nbta.that
        employment information requests both the number of years and actual
        years involved. Past interviewers report that it was easier.and smoother
        to obtain all crew members' names first and then request each individual's
        address and work history.

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                               16
Q13     Ask as written.  Respondents usually answer with a weekly or
        monthly consumption pattern.  If not , read the categories to him;
        probe to convert the consumption to weekly or monthly patterns.

Q14     Ask as written.

Q15     Ask as written.

Q16     Check all fish that R mentions.  Be sure to record any and all other
        fish not listed under "Other."

Q17     We are interested in R's family's fish consumption in comparison to
        himself.  For each family member/ read the choices to R, starting
        with his wife.  If. R does not have a wife, check #4 "Does not apply."
        Blanks have been provided to record each son/daughter's consuoptibn.
        Thus, if R has one daughter and two sons, the same information must
        be obtained for all three children.  If R responds to items $2 or 53,
        record how often in the blank.

Q18     If no, go to Q18a.  If yes, ask Q19-21 and record R's answers.  Probe
        for as accurate as possible information from the respondent.

QlSa    If no, go to Q25.  If yes, ask Q23-24.  Me are now interested in past
        srroking habits.  Again, probe for as accurate as possible information
        from the respondent.

Q25-61  Same specifications as Q18-24.

Q62-    This section of the questionnaire identifies 32 specific diseases.
 104
        For each of the diseases, R's response should indicate whether he, his
        wife, or his children have been told by a doctor that he or they had
        this disease. It is very important that a positive response from R
        be based on a doctor's diagnosis and not on his own suspicions.  The
        diseases are broken up into groups, i.e. lung diseases, liver diseases,
        etc.

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                               17.
        Starting with Q62, pronounce each disease slowly and clearly.
        After each disease, pause long enough for R to respond.  If R
        says no, record "1" in column one.  If R says yes, record  "2"
        in column one and obtain the following information:

              Column 2:   Family member with-disease  
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                                18
Q127     Record R's answer; natural children only.  If R has no natural
         children record "0" in the blank.

Q128     Record R's answer; step or adopted children only.  If R has no
         ste? or adopted children record "0" in the blank.

Q129     In this section we are interested only in R's pregnancies with the
         ccnnarcial fisherman.  Pregnancies from a'previous raarraige should
         not be included.  For each pregnancy, in the order they occurred/
         record the following:

               Column. 2:  Briefly describe any complications R experienced
                          during her pregnar.cy.  (e.g. excessive bleeding,
                          breech birth, etc.) If R experienced no corolications
                          record "None."
               Column 3:  Check the appropriate box for .the outcome of the
                          pregnancy                .       •
               Column 7:  Check appropriate box for infant's sex
               Column 8:  Date of infant's birth
               Column 9:  Live birth weight
              Colunsi 10:  Full name and address of hospital, clinic, or other
                          place of birth.  Obatin as specific an address as
                          possible
              Column 11:  Record attending doctor's full name

Q129a     If R answers no, go to Q130.  If R answers yes, obtain the following
          information:

               Column 1:  Record M or F for sex of child
               Column 2:  Record date of birth
               Column 3:  Record date of death
               Column 4:  Record cause of death and city and state where death
                          was reported
Q130
          If R answers no, go to Q131.  If R answers yes, obtain the following

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                                19
         infonnation for R or R's natural children, checking the appropriate
         boxes from left to right.   Record R's description of the birth
         defect in the last column (i.e.  cleft palate, mongoloidism, etc).

Q131     Check appropriate box.   If R answers no, proceed to next question.
         If R answers yes, go to Q132.

Ql31a    If R answers yes, go to Q134.  If R answers no, proceed to next
         question.

Q132     Check the birth control method most vised during R's adult life.

Q133     Record age at which R began to use birth control methods.

QD4     Record age at which R began to menstruate.

Q135     If R answers no, go to Q137.  If R answers yes, proceed to next
         question.

Q136     Check appropriate answer.   If cessation occurred due to surgery,
         obtain reason for surgery and full name .and address of doctor.  As
         always, obtain as specific as possible information.

Q137     if R answers yes, record medications prescribed by a doctor.   'If-R-
         cannot remember the name of the medications, suggest that she look
         at the bottles.  If she dees not have the bottles, record DK and-
         collect any other information possible—reason she takes Dedication,
         how often she takes it, etc.

Q138     Ask as written.

Q139     Ask as written.

Q140     Read the question and.choices to R.  Check appropriate response.

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                                20
Q141     If R answers no, go to Q143.  If R answers.yes, proceed to next
         question.

Q142     If R answers no, proceed to next question.  If R answers yes, obtain
         doctor's full narte and address.

Q143     Same specifications as Q137.
                      **************************

Page 22:  Observations by the Interviewer

          These observations are to be recorded by the interviewer immediately
following the interview.  While Ql and Q2 are strictly informational, Q3 and
Q4 CT 11 for the interviewer's judgement.  If you have difficulty in making
these judgements in the preceded terms, please write out your oonments and
depressions in the Garments section below.

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                              NON-INTERVIEW REPORT
                        GREAT LAKES FISHERMEN HEALTH STUDY
Respondent Name:	   Interviewer Name:

I.D.//:                                    Date:
        * A Non-Interview Report must be completed if the result of  the
          contact is "First Refusal", "Second Refusal", "Unavailable",
          "Other".

        * A Non-Interview Report must be completed if"the result of  the
          contact is "Respondent Moved" and the assignment is being  turned
          in to the supervisor.

        * A Non-Interview Report must be completed when an incomplete  assign--
          ment is returned after 12 or more contact attempts.
                                                 CIRCLE'APPROPRIATE  NUMBER

1.  With whom did you speak?   (FINAL CALL)
                                            Respondent	1
                                            Other household member.
                                                         (SPECIFY)	..2
                                            Neighbor...	;.....,	3
                                            Other  (SPECIFY)	_4.
                                            No one	 5
2.  What is the result of the contact for  this assignment?  Please  circle
    appropriate number.

    1  First/Second Refusal	  (Q3)     3  Respondent Moved	  (Qll)
    2  Unavailable	  (Q8)     4  Other	  (Q14)
                                           5  Maximum Calls....	.	  (Q16)

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Q3-Q7  For REFUSALS only.  PLEASE CHECK WHETHER THIS IS A FIRST OR SECOND
       .REFUSAL

       FIRST REFUSAL    	
       SECOND REFUSAL
3.  What were the reasons given, for the refusal?
4.  What do you think was the primary reason given for  the refusal?
5.  Was the refusal hostile, firm but NOT hostile, or mild?

                                           Hostile...	1   (End)
                                           Firm	2   (Q.6)
                                           Mild	3   (Q.6)


6.  Do you think another interviewer might be able to obtain the interview?

                                           Yes	1   (Q.7)
                                           No	2   (End)
                                           Perhaps	3   (Q.7)
7.  What type of interviewer do you think might be able to obtain the
    interview?
                                END

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 Q8-10   For UNAVAILABLES  only.

 8.   Why was the respondent  unavailable? (e.g*  no show, disconnected phone,
     respondent  deceased,  etc.)
 9.   When will  the repsondent be available for an interview?
LO.   Do  you think there were other reasons for the non-interview? (e.g.
     a polite refusal)   If so,  specify:
                                    END
  Qll-13  For RESPONDENT MOVED only.

LL.   How did you determine that the respondent had moved?
L2.   Did you obtain a new address?
13.   What is the respondent's new address?
                                               Yes.
                                               No..
                                    .1  (013)
                                    .2  (End)
     (Number)
(Street)
     (City)
(State)
(Zip)
     (Phone)
                                 END

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 Q14-15  For OTHER only.
14.  Explain fully the circumstances involved,   (e.g. language problem,
     evasive or suspicious, drunk or intoxicated, respondent was never
     a commercial fisherman, etc.)
15.  Would you suggest any special action for this assignment?  If so,
     specify:
                                  END


 Q16-18  For MAXIMUM CALLS only.

16.  How many attempts did you make  to  reach  the respondent?

                     Number of telephone  attempts	
17.  Did you ever speak  to anyone  in  the  respondent's household?

                                      Yes	.1  (Q18)
                                      No	2  (End)

18.  With whom did you speak  and what was (were)  the result(s) of your
     contacts?
                                      Respondent	1
                                      Other household member....2
                                      (SPECIFY) 	
                                      Other (SPECIFY)	3
      Result:
                                   END

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            APPENDIX IV
Proposed Set of Coding Instructions

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                          Coding Instructions
                  Great Lakes Fishermen Health  Survey


     The response materials  for the Great Lakes Fishermen  Health  Survey

will consist of three records per study subject.   The first  record  will

consist of the non-interview report materials which have a possible total

of 150 characters.  The second record will  consist of the  responses for

the first half of the interview.  Specifically, those questions directed

toward the commercial fishermen (licensee).  This record has a possible

total of 503 characters.  The third record will consist of the responses

for the second half of the interview.  Specifically, those questions

directed toward the spouse of the commercial  fisherman. This record has

a possible total of 89 characters.

     Unless otherwise specified,-.responses indicating a "don't know" answer

should be coded as a -1.  Coding space should remain blank in instances

where no information is available (i.e. a blank response space).   In instances

where an answer requires less coding space than that provided, the  spaces  to

the left should be filled with zeros (e.g. three spaces provided, answer  is

9 = JL JL JL  )•

     Partial  information, at a minimum, must include a name and city/state of

residence.

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                               Record I
                        (Non-Interview Report)

     .All  non-interview reports for each  study subject  should  be coded.
Space is  provided on the coding sheet for five  non-interview  reports  per
study subject.   Note that the subject ID number is  not repeated when  more
than- one  non-interview report is filed per subject.

1.  Study subject ID number.
    	 five digit code
    Each  study subject will  receive a five digit identification number.
2. . Interviewer ID number.
    	 two digit code
    Each  interviewer will receive a two  digit identification  number.
3. 'Date  of non-interview report.
    	/	/	six digit code
    Code  exact month, day and the last two digits of the  year.
4.  (Question 1).  Contact status at the time of call.
    	 single digit code
    1 - respondent                 4 = other
    2 = other household member     5 = no one
    3 = neighbor
5.  (Question 2).  Result of the contact for the assignment.
    	 single digit code
    1 = first refusal              4 = respondent moved
    2 = second refusal             5 = other
    3 = unavailable                6 = maximum number  of  calls
    NOTE:  If "maximum number of calls"  is coded than  Questions 16-18
           should be coded.   Questions 3-15 should  be  blank.
6.  (Question 3).  Reason for refusal.
    	/	/	three digit code
    First digit corresponds  to a reason of "not interested".
    Second digit corresponds to a reason of "not available-at the time  of  call",
    Third digit corresponds  to "other" reason.
    Code  "1" if mentioned as a reason for refusal.
    Code  "0" if not mentioned as a reason for refusal'.

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 7.   (Question 4).   Primary reason  for  refusal.

     	/	/	 three  digit code

     First digit  corresponds to a reason of  "not  interested".
     Second  digit corresponds to a  reason of "not  available  at  the time of call".
     Third digit  corresponds to an  "other" reason.

     Code "1"  if  mentioned as a reason  for refusal.
     Code "0"  if  not mentioned as a reason for  refusal.

 8.   (Question 5).   Type of refusal.

     	 single digit code

     1  =  hostile

     2  =  firm

     3  =  mild

     Code type of refusal indicated by  interviewer.

 9.   (Question 6).   Interviewer determination if  subject  interview is  obtainable
     by means  of  another interviewer.

     	 single digit code

     1  » no

     2  « yes

     3  « perhaps

10.   (Question 8).   Reasons why study subject was  unavailable at  the time of
     call.

    .__/__/	/	/	' five digit code

     First digit  corresponds to "not home at time  of  call".
     Second  digit corresponds to "study subject has moved".
     Third digit  corresponds to "study  subject  is  deceased".
     Fourth  digit corresponds to "phone has  been disconnected".
     Fifth digit  corresponds to "other".

     Code "1"  1f  determined as a reason why  subject was unavailable.
     Code "0"  if  not determined as  a reason  why subject was  unavailable.

     NOTE:  Question 8 should only  be coded  when the  answer  to  Question 2 is
            "unavailable",  (i.e.,  "3").

11.   (Question 12).  Was a new address obtained.

     	 single digit code

     1  = no

     2  *= yes

     NOTE:  Question 12 should only be  coded when  the answer to Question  2--fs
            "respondent moved",  (i.e., 4).

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10   (Question 14).   "Other" reasons for non-interview report.
     	 two digit code
     Code the total  number of "other" reasons  wrjtten  by  interviewer.
13.   (Question 16).   How many attempts did  you ma Ice  to reach  subject.
     __-	two digit code
     Code.number of  attempts written by interviewer.
     NOTE:   Question 16 should only be coded when  the  answer  to  Question  2
            is "maximum calls",  (i.e., 6).
14-.   (Question 17).   Did interviewer speak  to  any  household member.
    	 single digit code
     1 =  no
     2 =  ves

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                               Record  I

First Non-Interview Report.
    1.	
    2.
    3.	/	/	
    4.   (Question 1}   	
    5.   (Question 2)   	
    6.   (Question 3)
    7.   (Question 4)
    8.   (Question 5)
    9.   (Question 6)
   10.   (Question 8)   	
   11.   (Question 12)    _
   12.   (Question 14)    	
   13.   (Question 16)    	
   14.   (Question 17)    	
                                   (34 Characters)    Total =34 Characters

Second Non-Interview Report.
    2,	
    3,	/	/	
    4.   (Question 1)   	
    5.   (Question 2)   	
    6.   (Question 3)   _/_/_
    7.   (Question 4)   	/	/	
    8.   (Question 5)   __
    9.   (Question 6)   _^_
   10.   (Questions)   __/__/_/__/_

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   11.  (Question 12)   _
   13.  (Question 16)   __
   14.  (Question 17)   _
                                   (29 Characters)     Total  = 63  Characters

Third Non-Interview Report.
    2.  __
    3.  __ / __ /___
    4. ._
    5.  _
    6.
    7.
    8.
    9.
   10 •
   11.  _
   12.  __
   13.  __
   14.  _
                                   (29 Characters)     Total  = 92  Characters

Fourth Non-Interview Report.
    2.  __
    3.  __ / __ / __
    4.  _
    5.
    7-
    S.
    o

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   10-  _/_/_/_/_
   11-  _
   12.	
   13.	
   14.  	
                                  (29 Characters)    Total = 121 Characters

Fifth Non-Interview  Report
    2.	
    3.	/	/	
    4.   (Question  1)  	
    5.   (Question  2)  	
    6.   (Question  3}  	/	/	
    7.   (Question  4)  	/	/	
    8.   (Question  5)  	
    9.   (Question  6)  	
   10.   (Question  8)  _/_/_/_/_
   11.   (Question  12)    __
   12.   (Question  14)    	
   13.   (Question  16)    	
   14.   (Question  17)    	
                                  (29 Characters)    Total = 150 Characters

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•Subject ID number	
 Name of coder  	
 Date	
                                   Record  II
                      Great Lakes  Fishermen Health Survey

 1.   Study subject ID number.
     	 five  digit  code
     Each subject win  receive  a  five  digit identification numoer.
 2.   Interviewer ID number.
     	 two  digit code
     Each interviewer will  receive a two digit  identification number.
 .3.   Length of  interview.
     	three digit code
     The length of time required  for the interview will  be calculated by the
       coder.   Code the length  of time in  minutes.
 4.   (Question  2).  Birthdate of respondent.
     	/	/	six digit  code
     lode month, day, and the last two digits of the  year.
 5.   (Question  3).  Marital status of  respondent.
     	 single  digit code
       1 = sinqle (never  married)^      3 = separated  or  divorced
       2 = married                     4 = widowed
 6.   (Question  126).  Sex of respondent.
     	.single  digit code
       1 = male                        2 = female
 7.   (Question  4).  Number of children of  study respondent.
     	/	 two  digit code
     First digit corresponds to the number of sons.
     Second digit corresponds to  the  number of  daughters.
 8.   (Question  5).  Does  respondent currently own a  commercial  fishing license.
     	/	/_	four digit code
     First digit corresponds to the direct answer of Question  5.
       1 = no                          2  = yes
     Second digit corresponds to  the  number of  different periods  of  time the
       respondent has held a commercial fishing license.

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     Third and fourth digits correspond to the total  number of years the study
       participant has held a license.
     NOTE:  If the first digit is "1"  corresponding to a "no" answer, than the
            remaining spaces should be  blank.
 9.  (Question 6).  Did respondent ever hold a commercial  fishing license.
       I	/	four digit code
     Coding instructions identical to  those presented for number 8 (Question 5).
10.  (Question 7).  Did respondent ever crew or be a  partner with an individual
     owning a commercial~~fishing license.
     	/	/	four digit code
     Coding instructions identical to  those presented for number 3 (Question 5).
     NOTE:  If Question 5-7 have been  answered "no",  than the rest of Record II
            and Record III (coding spaces) are blank.
11.  (Question 8).  Lake and surrounding land masses  will  be stratified into segments.
     	/	two digit code
     First digit corresponds to the Lake.
       1 - Superior                    4 = Erie
       2 = Michigan                    5 = Ontario
       3 = Huron
     Second digit corresponds to the lake segment.
     NOTE:  See appendices for lake stratifications.
12.  (Question 9).  Is commercial fishing respondents current full-time occupation.
     	/	/	four digit code
     Coding instructions are identical  to those presented for number 8 (Question 5).
13.  (Question 10).  Current full-time occupation of respondent.
     	 single digit code
       1 = Occupation known to have potential  exposure to hazardous materials.
       2 = Occupation subject to have  potential exposure to hazardous materials.
       3 - Occupation not known to have potential exposure to hazardous materials.
       4 = Occupation unclassifiable.
     NOTE:  See appendices for classification of occupations.
14.  (Question 11).  Was commercial fishing ever respondents full-time occupation.
     	/	/	four digit code
     Coding  instructions are identical  to those provide for number 8 (Question 5).

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IS.  (Question 12).  Total number of names/addresses  of full-  or  part-t.ime
     crew members.
     	 two digit code
     Code- number  of names/addresses obtained from respondent.
     NOTE:  Partial information should be coded as if the information were  complete.
16.  (Question 13).  How many times does respondent consume  Great Lakes  fish.
     	 single digit code
       1 = n.ever                       5 =   1 time per month
       2 .= 1-2 times per week          i - 1 time per month
       3..= 3-5 times per week          7 = 2-3 times  per month
       4.,.= 6-7 .times per week
.17...  (Question 14).  How many years has respondent consumed  Great Lakes  fish with
     this' fr-equency.
     	 two digit code
     Code number of years given by respondent.
18.  'Question 15).  How many pounds of fish does study participant  consume per
     i.ieal.
     	 single digit code
     Code response in terms of pounds of fish per meal.  The response can  be
       coded in terms of a fraction of a pound.
19.  (Question 16).  What types of Great Lakes fish does respondent  consume most
     often.
     _/__/_/_/_/_/_/_/_/_/_/_/_/_/_ fifteen digit code.
       0 = not mentioned by respondent
       1 = mentioned by respondent
       First digit = salmon            Ninth digit = lake trout
       Second digit = perch            Tenth digit = smelt
       Third digit = walleye           Eleventh digit = bass
       Fourth digit = burbot           Twelveth digit = lake herring
       Fifth digit = rough fish        Thirteenth digit = pan  fish
       Sixth digit = chub              Fourteenth digit = other trout
       Seventh digit = northern pike   Fifteenth digit = other
       Eighth digit = lake whitefish
     NOTE:  All spaces provided should be coded with either  a  "0" or a  "1".
20.  (Question 17).  How often do the family members of respondent consume fish.
     	/	/	/	/	/	 six digit code

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      First digit corresponds to the consumption patterns of the spouse.

        1 = as often as respondent      4 = does not apply

        2 = more often than respondent  5 = never

        3 = less often than respondent

      Second-sixth digits are for additional  family members when appropriate.

      NOTE:  Spaces should be left blank if there are no family members and should
             be coded with a "-1" in those instances where a "don't know" is given
             as a response.

      (Question 18-24).  Cigarette smoking history.

 21.  	/	 two digit code

      First digit corresponds to whether or not the respondent smokes cigarettes
        currently.
      Second digit corresponds to whether or not the respondent smoke cigarettes
        in the past.

        1 = no

        2 = yes

      NOTE:  Both digits should be coded.  If both digits correspond to "no" than
             the coding spaces for 21a should remain blank.  If either, of the digits
             correspond to "yes" than-'-the spaces for 21a should be coded.

21a.	/	/	six digit code

      First and second digits correspond to the age at which the respondent began-to
        smoke cigarettes.
      Third and fourth digits correspond to the average number of cigarettes the
        respondent smokes (had smoked) per day.
      •Fifth and sixth digits correspond to the number of years the respondnet smoked
        cigarettes (with this frequency).

      NOTE:  Twenty-one a should be blank if 21 is coded _i_/_l_ .   Twenty-one a should
             be coded if either digit in 21 is coded with a "2".

      (Question 25-31).  Pipe smoking history.

 22.    /	 two digit code

      First digit corresponds to whether or not respondent smokes a pipe regularly.
      Second digit corresponds to whether or not respondent smoked a pipe in the past..

        1 = no

        2 a yes

      NOTE:  Both digits should be coded.  If both digits are answered- "no" than, the
             coding spaces for 22a should remain blank.  If either of the digits-
             correspond to "yes", than the spaces for 21a should be coded.-

22a.	/	/	six digit code

      First and second digits correspond to the age at which respondent began to smoke
        a pipe.

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      Third and fourth digits correspond to the number of pipefuls  the  respondent
        smokes (had smoked) per day.
      Fifth and sixth digits correspond to the number of years  the  respondent  smoked
        a pipe (,w.ith this frequency).

      .NOTE:. Twenty-two a should be blank if 22 is coded _!_/ 1 .   Twenty-two  a  should
             be coded if either digit  in 21 is coded with a T".

      (Questions 32-38).  Cigar smoking history.

 23-   __/_ tw° digit code

      First digit corresponds to whether or not the respondent  smokes cigars
        currently.
      Second digit corresponds to whether or not the respondent smoked  cigars  in
        the past.

        1 = no

        2 - yes

      NOTE:  Both digits should be coded.  If both digits correspond to a  "no"  than
             the coding spaces for 23a should remain blank.   If either  of  the  digits
             correspond to "yes" than  the spaces for 23a should be  coded.

22a.	/	/	six digit code

      First and second digits correspond to the age at which  the respondent  began
        to smoke cigars.
      Third and fourth digits correspond to the average number  of cigars the respon-
        dent smokes (had smoked) per day.
      Fifth and sixth digits correspond to the number of years  the  respondent
        smoked cigars (with this frequency).

      NOTE:  Twenty-three a should be  blank if 23 is coded _!_/_!_.  Twenty-three a
             should bfi coded if either digit in 23 is coded with a  "2".

 24.   (Question 39).  Has the respondent ever chewed tobacco  regularly.

      	single digit code

        T = no

        2 = yes

 25.   (Question 40).  Has the respondent ever used snuff regularly.

      	 single digit code

        1 = no

        2 = yes

      (Question 41-47).  Liquor drinking history.

 26.   	/	 two digit code

      First digit corresponds to whether or not the respondent  drinks  liquor
        regularly.

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      Second digit corresponds  to whether  or  not  the  respondent  drank  liquor in the
        past.

        1 = no

        2 » yes

      NOTE:  Both digits should be  coded.   If both  digits  correspond to "no", than
             the coding spaces  for  26a  should remain  blank.   If  either of the digits
             correspond to "yes"  than the  spaces  for  26a  should  be  coded.

26a.	/	/	six digit code

      First and second digits correspond to the age at which  the respondent began to
        drink liquor.
      Third and fourth digits correspond to the average number of hard liquor drinks
        the respondent consumes (had consumed) per  week.
      Fifth and sixth digits correspond to the number of  years the  respondent drank
        hard liquor (with this  frequency).

      NOTE:  Twenty-six a should  be blank  if  26 is  coded  _!_/_!_.   Twenty-six a should
             be coded if either digit in 26 is coded  with a "2".

      (Questions 48-54).  Beer  drinking history.

 27.   	/	two digit code

      First digit corresponds to  whether or not the respondent drinks  beer currently.
      Second digit corresponds  'to whe-ther  or  not  the  respondent  drank, beer in the
        past.

        1 = no

        2 = yes

      NOTE:  Both digits should be  coded.   If both  digits correspond to "no", than
             the coding spaces  for  27a  should remain  blank.   If  either of the digits
             correspond to "yes8,  than  the spaces for 27a should be coded.'

27a.	/	/	six digit code

      First and second digits correspond to the age"at which  respondent.began to
        drink beer.
      Third and fourth digits correspond to the number of beers  the respondent
        drinks  (had drunk) per week.
      Fifth and sixth digits correspond to the number of  years the  respondent
        drank beer (with this frequency).

      NOTE:  Twenty-seven a should  be  blank if 27 is  coded _1_/J_.  Twenty-seven a
             should be coded if either  digit  in 27  is coded with a  "2".

      (Questions 55-61).  Wine drinking history.

 28.  _/__ two digit code

      First digit corresponds to whether or not the respondent drinks  wine currently.
      Second digit corresponds to whether  or  not  the  repsondent  drank  wine in the
         past.

         1  = no

         2  = yes

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     f.'OTE:  Both digits should be_coded.   If both digits  correspond  to  "no"  than
            the coding spaces for 28a should remain blank.   If either of  the digits
            correspond to "yes",  than the spaces  for 28a  should be coded.
     -_^ •__/__ _V__ _ six digit  code
     First and second digits correspond to the age at which  respondent  began to
       drink wine.
     Third and fourth digits correspond to the number of  wine drinks the  respondent
       consumes' (had consumed) per week.
     Fifth and sixth digits correspond to the number of years the respondent dranic
       winre(with this frequency).
     NOTE:  Twenty-eight a should be blank if 28 is coded _!_/_!_. '  Twenty-eight a
            should be coded if either digit in twenty-eight is  coded  with  a  "2".
     (Questions 62-105).  Familial  medical conditions of respondent.
29.  _/_/_/_/_ five digit code
     -First digit corresponds to the acknowledgement of a disease/condition
       variable within the family.
       1 = no
       2..= yes
     NOTE:  If the first digit is coded with a "1" than the remaining coding spaces
            should remain blank.  If the first digit is coded with a  "2",  than
            the remaining digits must be coded.
     Second digit corresponds to the family member with the disease/condition.
       1 = self                        3 = son
       2 = spouse                      4 = daughter
     Third digit corresponds to whether or not information is provided regarding  the
       name(s) and address(es) of medical personnel and/or service.  Partial informa-
       tion should be coded as if information were complete.
       1 • = no
       2 = yes
      -1 = don't know (no information given)
     Fourth digit corresponds to whether or not information is  provided regarding
       the date of diagnosis.  Partial information should be coded as if information
       were complete.
       1 = no
       2 = yes
      -1 = don't know (no information given)
     Fifth digit corresponds to whether or not the disease/condition  was verified
       by medical record.
         1 = no
         2 = yes
     NOTE:  The five digit coding sequence  (i.e.., _ / _ / _ / _ / _ )  and the instructions
            for each of the digits are to be USP^ for Questions 62-104.

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     (Question 105-125).   Symptomatology of respondent.
72.  	/	/	/__/	/	/	eight digit code
     First digit corresponds to the acknowledgement of a  symptom experienced  by
       by the respondent.
       1 = no
       2 = yes
     NOTE:  If the first  digit is  coded with a "1"  than  the remaining coding  spaces
            should remain blank.  If the first digit is  coded with  a  "2",  than the
            remaining digits must  be coded.
     Second digit corresponds to whether or not information regarding the  frequency
       of symptoms was given by the respondent.
       1 = no
       2 = yes
      -1 = don't know
     Third digit corresponds to whether or not information  regarding-  the duration
       of symptoms was given by the respondent.
       1 = no
       2 = yes
      -1 = don't know
     Fourth digit corresponds to whether or not respondent  consulted  a physician.
       1 a no
       2 = yes
     NOTE:  If the fourth digit is coded with a "1" than  the remaining digits should
            be blank.  If the fourth digit is-coded with  a  "2",  than  the fifth
            through eighth digits  must be coded.
     Fifth digit corresponds to whether or not information  is provided regarding the
       name(s)/address(es) of medical  personnel and/or service.   Partial information
       should be coded as if the information were complete.
       1 = no
       2 3 yes
      -1 = don't know
     Sixth and seventh digits correspond to the last two  digits  of  the year .the
       symptom was most recently experienced (e.g., 1979  =_7__9_).-  Code -1 -1- if
       respondent doesn't know.
     Eighth digit corresponds to whether or not the symptom was  verified by medical
       record.
       1 = no
       2 = yes
     NOTE:  The eight digit coding sequence (i.e.,  _/__/__/	/__/__/	) .and the
            instructions  for each  of the digits are to be used for  Questions  105-125,

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                                      Record  II

                      1.  _____ ____
                      2,  . __
                      '3. ; ___ _•
(Question  ?.}           4-  __ / __ / __
(Question  3)           5.  _
(Question  126)         6.  __
(Question '4)           7.  _ / _
(Question  5)           8.  __/__/ __
( Qu es t i o ri 5 )           9 .  __/__/ __
(Question  7)           10.  __/ _ /; __
(Questions)           11.  __/ _
(Question  9)           12.  __/__/__
(Question  10)          13.
(Question  11)          14.  _/__/ __
( Quest io.n, 12)         .15.  __
(Question  13)          16.  _
(Question  14)          17.  _ _
(Question 15)          IS.  _
(Question 16)          19.  _/_/__/_/_/_/_/_/_/_/_/_/_/__/.
(Question 17)          20.  __/___/___/__/__/__
                      21.'   /
                  ^
(Question 18-24) <
                  \
                   .   22.     /
 (Question 25-31)
                 ^  .22a.   __•/_   /
                      23.     /
 (Question 32-38)
                  " -23a.        /     /

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(question 39)
(Question 40)

(Question 41-47) <

(Question 48-54) <

(Question 55-61 )<
(Question 62}
(Question 63).
(Question 64)
(Question 65)
(Question 66)
(Question 67)
(Question 68)
(Question 69)
(Question 70)
(Question 71)
(Question 72)
(Question 73)
(Question 74)
(Question 75)
(Question 76)
(Question 77)
(Question 78)
(Question 79)
(Question 80)
(Question 81)
(Question 82)
24. _
25. _
/>* 25< _/_
X
\25a . 	 / 	 / 	
\27a / /
/^ 28. __/__

29. _/__/__/__/_
30 • __/_/_/__/_
31. _/__/__/__/_
32 • _/__/_/„/_
33. __/__/__/__/_
34 • _/_/_/„/_
35 • _/_/__/_/_
36 • _/_y_-/__/_
37 • -_*L_/__/.__/_
38. _/_/__/__/_
39. _/_/_/_/_
40 - ^/__/_jL_/_
41 . __/__/_/„/__
«. __/„/„/„/_
43 • __/_/__/_/_
44 . _J_J_J_I 	
45 • __/_/„/_/__
46. _/__/_/_/_
47 . _/__/__/__/_
48 . _/__/_/_/_
49. (Iff

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 (Question 83)
 (Question 84)
 (Question 85)
 (Question .8.6)
 (Question-87)
 (Question 88)
..(Question 89)
 (Question 90)
 (Question. 91.)
 (Question 92.)
 (Question 9.3)
 (Question 94)
 (Question 95)
 (Question 96)
 (Question 97)
 (Question 98)
 (Question 99)
 (Question 1.00)
 (Question 101)
 (Question 102)
 (Question 103)
 (Question 104)
 (Question 105)
 (Question 106)
 (Question 107)         74.  _/_/_/_/__/__/__/_
 (Question  108)         75.  _/__/_/_/__/__/	/.
 (Question 109)         76.
 (Question 110)         77,
 (Question  111)         73.
73.     /_/  /_/__/_/__/
     _/_/__/_/_/_/_/_
     _/__/__/_ /_/_/_/_

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(Question 112)        79.  _/_/__/_/__/_/_/_



(Question 113)        80.  __/_/__/__/_/__/__/_



(Question 114)        81.  _/__/_/_/_/__/_/__



(Question 115)        82.  _/_/__/_/_/_/_/_



(Question 116)        83.  __/_/__/„/__/„/__/_



(Question 117)        84. - _/__/_/_/_/_„/_/„



(Question 118)        85.  _/_/__/_/_/_/__/_



(Question 119)        86.  _/__/__/__/_/__/„/_



(Question 120)        87.  _/„/__/_/_/__/__/_



(Question 121-}        88.  _/_/„/„/_/__/_./__



(question 122)        89.  _/__/__/_/_/__/__/_



(Question 123)        90.  _/_/„/_/_/„_/_/__



(Question 124)        91.  _/__/_/_/__/__/__/„



(Question 125)        92.  _J „! _J _J _J _J _J __
                                                     Total  =  503 Characters

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                                 .Record  III
                      Great Lakes  Fishermen Health Survey

    93.   (Question. 127).   Number of natural children of the respondent.
         	two digit  code
         Code number of children given  by respondent.
    94.   (Question 128).   Number of step  or adopted children of the respondent.
         	 two digit  code
         Code number of children given  by respondent.
95-100.   (Question 129).   Information regarding  pregnancies and pregnancy outcomes
         of respondent.
         _/_/_/_/_/__/_/__/	nine  digtt code
         First digit corresponds to the order of pregnancies of the respondent.
           (e.g., Third pregnancy  = 3 ).
         Second digit corresponds  to whether  or  not there were any complications
           during this pregnancy.
           1 - no
           2 = yes
         Third digit corresponds to the outcome  of the  pregnancy.
           1 = miscarriage             3  = live  birth
           2 = stillbirth              4  = other (abortion)
         NOTE:  If the" third digit is coded  "1"  or "4"  than the fourth,.fifth, and
                sixth digits should be  blank.
         Fourth digit corresponds  to the  infants sex.
           1 = male
           2 = female
         Fifth digit corresponds to whether or  not a  date of  birth is  provided by
           the respondent.
           1 = no
           2 * yes
         Sixth digit corresponds to whether or  not a  live birth weight is  provided
           by the respondent.
           1 - no
           2 = yes
         NOTE:  The third digit must be coded with a  "2".   If  the third digit  is
                coded with a number other than  a "2"  than this  space  should  be
                blank.

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      Seventh digit corresponds  to  whether or  not information  is  provided
        regarding the name(s)/address(es)  of hospital  or place of birth.

        1  = no

        2  - yes

       -1  = don't know

      Eighth digit corresponds  to whether  or not  information  is provided
        regarding the name(s) of the  attending physician(s).

        1  = no
        2  = yes

       -1  =• don't know

      Ninth digit corresponds to whether or not the pregnancy  and outcome  was
        verified by medical  record.

        1  = no-

        2  = yes

      NOTE:  There are spaces provided for six pregnancies  on  the coding sheet.
             If more than six pregnancies, code the first six  pregnancies  1-isted
             on the questionnaire.

101.   (Question 129a).

      :	/__/_ three digit code

      First digit corresponds to whether or not any of the  respondents  natural
        children have died.

        1  = no

        2  = yes

      NOTE:  If the first digit is  coded "1" than the  second  and  third  digits
             should be blank.   If the first digit is coded  with a "2" than the
             second and third digits  must  be coded.

      Second digit corresponds  to whether  or not  any information  regarding the
        child's sex, date of birth, date of death, name, cause of death, and
        the city and state in which the death was reported.

        I  = no

        2  =• yes
      NOTE:  Partial information should be considered  as a  "yes"  answer.   The
             most important pieces  o.f information are  the name of the child and
             the city and state in  which the death was reported.

      Third digit corresponds to whether or not the death certificate was
        obtained from the respective  state agency.

        1  = no

        2  = yes

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102.  (Question 130).
       	/	two digit code
       First.digit corresponds to whether or not the .respondent.or. any of  the
         respondent's  natural  children have birth defects.
         1 = no
         2 = yes
       NOTE:  If the first digit is coded with a "1" than the second  digit should
              remain blank.  If the first digit is coded with a  "2" than the
              second digit must be coded.
       Second digit corresponds to whether or not information is provided  regard-
         ing the relationship to the respondent, the current status  (i.e. ..living
         or dead) of the individual, and the nature of the birth defect.
         1 = no
         2 * yes
       NOTE:  Partial  information should be considered as a "yes" response.
              The most important piece of information is the nature of the birth
              defect.
 103.  (Questions 131-131a).
       	/	two digit code
       First digit corresponds to whether or not the respondent  uses  a birtti
         control method in the past.
         1 = no
         2 - yes
       NOTE:'  Both spaces should be coded.
 104.  (Question 132).  What method of birth control was most frequently used
       during the respondent's adult life.
       	 single digit code
         1 = pill                     5 - rhythm
         2 = I.U.D.                   6 = condom
         3 = diaphram                 7 = two of the above
         4 = foam or jelly            8 = other
 105.  (Question 133).  Age at which' respondent began to use birth control methods.
       	/	 two digit code
       Code age given by respondent.
 105.  (Question 134).  At what age did the respondent begin to  menstruate.
       	/	 two digit code

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107.   (Questions 135-137).
      _/__/__/_/_/	six digit  code
      First digit corresponds to whether or not  the respondent has  stopped
        menstruating.
        1 = no
        2 = yes
      NOTE:  If the first digit is coded "1"  than  the  second,  third and  fourth
             digits should remain  blank.  If the first digit is coded  "2"  than
             the remaining digits  should be coded.
      Second digit corresponds to  whether the menstrual  cessation was  natural
        or due to surgery.
        1 = natural
        2 = surgery
      NOTE:  If the second digit is coded with a "1" than  the  third digit  should
             be blank.  If the second digit is coded with  a  "2" than the third
             digit should be coded.
      Third digit corresponds to whether or not  information  is given regarding
        the reason for surgery and the  attending physician's name and  address.
        1 = no
        2 = yes
       -1 = don't know
      NOTE:  Partial information should be considered  as a "yes" response.
      Fourth digit corresponds to  whether or not the respondent to  any medica-
        tions since menstruation began.
        1 = no
        2 = yes
      Fifth digit corresponds to whether.or not  information  is provided  regarding
        the names of the medication.
        1 = no
        2 = yes
      NOTE:  Partial information should be coded as a  "yes"  response.
      Sixth digit corresponds to whether or not  surgical cessation  of  menses was
        verified by medical record.
        1 = no
        2 = yes
108.  (Question 138).  What was the average number of  days between  the respondent's
      periods.
        	 two digit code
      Code average number of days.

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 109.   (Question  139).  What was the average length of the respondent's period.
       	 two  digit code
       Code  average  number of days.
 ITO.   (Question  140).  Has;respondent's  blood  flow during menstruation changed .
       in  the last five years.
       	  single  digit code
        1 = increased                  3 = stayed the same
        2 = decreased                  4 = does  not apply
.111.   (Question  141).
       __/	/__/__ four digit code
       First digit corresponds  to whether or not  the respondent experience any
        •spotting or bleeding between menstrual cycles.
        1 f. no
        2 = yes
       HOTE:  If  the first digit  is coded with  a  "1" than the remaining spaces
              should be blank.  If the  first digit is coded as a "2" than the
              second digit must be coded.
       Second digit  corresponds to whether or not the respondent consulted a
        .physician.
        1 = no
        2 = yes
       NOTE:  If  the second digit is coded "1"  than the  remaining spaces should
              be  blank.   If the second  digit is coded with a "2" than the
              remaining digits  must be  coded.
       Third digit corresponds  to whether or not  information'is provided regarding
        the name(s) and  address(es) of physicians consulted for this problem.
        1 = no
        2 = yes
       NOTE:  Partial information should  be considered as a "yes" response.
       Fourth digit  corresponds to whether or not respondent's condition was
        verified by medical record.
        1 = no
        2 * yes
 112.   (Question  143).
       	/	 two  digit code
       First digit corresponds  to whether or not  the respondent used medications for
        menstrual irregularities.
        1 = no
        2 = yes

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      Second digit corresponds to whether or not information is  provided by
        respondent regarding the name(s)  of the medication(s).
                            i
        1 = no
        2 =* yes
       -1 = don't know
      NOTE:  Second digit should be blank if the first  digit is  coded  with  a  "1".
113.  (Questions 1-126).   Were answered by:
      	 single digit code
        1 = study subject
        2 = spouse of study subject
        3 = other
114.  (Questions 127-143).  Were answered by:
      	 single digit code
        1 = study subject
        2 = spouse of study subject
        3 = other

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                                    Record III
(Question  127)        93.
(Ques.tiQn.128)        94.
                     95.

(Question  129)
                            .J-J-J-J-J-J-J-
          129a)
          130)
          131-131a)  103.
(Question
(Question
(Question
(Question
(Question
(Question
(Question
(Question
(Question
(Question
(Question  141-142)   111.  __/_/_/_
                            /_/_/_/_/_/_/_/,
                            /__/_
         132}
         133)
         134)
         135-137)
         138)
         139)
         140)
                    104.
                    105.
                    106.
                    107.
                    108.
                    109.
                    110.
(Question  143)
                    112.
                    113.
                    114.
                                                            Total = 89 Characters

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   CODES TO BE USED FOR OCCUPATIONS (QUESTION 10)


01 = Professional
02 = Officials and Managers
03 = Sales Workers
04 = Clerical Workers
05 = Craftsmen
06 = Foremen
07 - Operatives — non transport
09 =» Operatives - transport equipment
10 =» Laborers - unskilled
11 = Unemployed
12 = Disabled
13 = Retired
88 = Not Reported
14 = Student
15 = Military
16 = Farm Managers or Farmers
17 = Service Workers  (except Private Household Workers)
18 = Private Household Workers
     U.S. Bureau of Census:  1970 Census of Population  .
     Classified Index of Industries and Occupations.
     U.S. Government Printing Office, Washington, D.C., 1971

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       CODES FOR CAUSES OF. DEATH  (QUESTION  I29a)

'0-1- = Diseases of heart
02 = Malignant neoplasms  (tumor,  leukemia,  carcinoma)
03 = Cerebrovascular diseases
04 = Accidents
'05 = Influenza and pneumonia
06.= Tuberculosis, all forms
07 = Diabetes mellitus
08 = Bronchitis, emphysema, and asthma
09 = Cirrhosis of liver
10 = Suicide
11 = Congenigal anomalies
12 = Homicide, War
13 = Nephritis and nephrosis
14 = Peptic ulcer, Hemorrhaging
15 = Other Vascular  Diseases
16 = Old  age
17 = Other.- Alcoholism
99 = Unknown

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   CODES TO BE USED FOR BIRTH DEFECTS (QUESTION 130)
1 = Central nervous system -
    l=Anencephaly      3=Hydrocyphalus       5=Encephalocele
    2=Spina Bifida     4=Microencephaly      6=not specified

2 = Craniofacial -
    l=Cleft palate         3=Congenital cataract   5=not specified
    2=Cleft lip + palate   4=Anopthalmus

3 = Cardiovascular -
    l=Transposition       3=Coarctation       5 not specified
    2=Tetralogy           4=Ventricular septal
                            defect

4 = Gastrointestinal -
    l=Tracheo-esophageal atresias   4=Pyloric stenosis
    2=Small bowel atresias          5=Diaphramatic hernia
    3=Anorectal atresias            6=0mphalocele
                       7=not specified

5 = Genitourinary - .
    l=Exstrophy of bladder     3=Hypospadias
    2=Septic kidney disease    4=not specified

6 = Musculoskeletal
    l=Club foot                 4=Polydactyly
    2=Reduction deformities     5=Syndactyly
    3=Dislocated hips           6=not specified

7 = Chcomosomal -
    l=Down Syndrome         3=Trisomy E
    2=Trisomy O             4=not specified
Codes adapted from - Center for Disease Control:  Congenital
Malformations, Surveillance, July 1978 - June 1979, issued
July 1980.

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               APPENDIX V
  Proposed Set of Analytical Procedures
(appropriate for pilot questionnaires  only)

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Ql.    Check answer against data file and correct data file if
       necessary.

Q2.    Categorical response will be used to stratify participants
       in analysis.   (e.g., search males for prostate cancer.)  A
       frequency count to describe the cohort will be performed.

Q3.    Categorical response will be used to stratify participants
       in analysis.   (e.g.? search Blacks for heart disease.)  A
       frequency count to describe the cohort will be performed.

Q4.    Birthdate will be utilized for:
          a) determining age distribution of the cohort
          b) determining age of the participant
          c) to determine the age of the participants at the given
             life events of Qll, Q13, Q67, to Q118

Q5.    Answer will be utilized to obtain medical records and death
       certificates when applicable.

6.     Categorical response will be used to stratify participants
       in analysis.   (e.g., search marrieds for lung cancers.)  A
       frequency count to describe the cohort will be performed.

Q7.    Answer will be used to"determine years of possible exposure
       potential.  Reliability will be determined by comparison with
       Qll, Q12, and Q13 and available state records.

Q8.    Answer will be used to determine years of possible exposure
       potential.  Reliability will be determined by comparison with
       Qll, Q12, and Q13 and available state records.

Q9.    Answer will be used to.determine years of possible exposure
       potential.  Reliability will be determined by comparison with
       Qll, Q12, and Q13 and available state records.

       NOTE:  Q7, Q8, and Q9 will serve as exclusion criteria.  If
       the  answer is  "no" for the three questions participants will
       be excluded from the analyses.

QIC.   Each lake will be stratified into segments.  Each segment
       will be environmentally characterized according to the degree
       of contamination with PCBs and other organics.  Characteriza-
       tion will be  based upon available environmental data regard-
       ing 'organics  in water, sediments, and fish.  Fishing location
       will be used  to determine exposure potential.

QlOa.  Answer will be used in conjunction with Q10 to determine
       expsure potential.

       NOTE:  QlOa is not found in Protocol III.

Qll.   Answer will be used to determine years of possible exposure
       potential.  Reliability will be determined by comparison -with
       Q7,  Q8, and Q9 and available state records.

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Q12.   Answer will be used to determine if a possible confounding
       exposure is occurring through a secondary occupation.

Q13.   Answer will be used to determine years-of possible exposure
       potential.  Reliability will be determined by comparison with
       Q7, Q8, and Q9 and available state records.

Q14.   Answer will be used to determine if sport fishing and poten-
       tial sport fish consumption may be an additional route of
       exposure.  A frequency distribution on this variable will be
       •performed.

Q15.   Answer will be used to determine if sport fishing and poten-
       tial sport fish consumption may be an additional route of
       exposure.  A frequency distribution on this variable will be
       performed.

Q16.   Answer will be used to determine in which states and what
       types of waterbodies study participants sport fish.

Q17.   .Answer(s) will'be:
         a) added to the data file for compiling a future cohort
         b) a frequency distribution will be compiled regarding
            the number of names listed per questionnaire.

Q18.   Answer is a dichotomous variable that will be used to:
         a) serve as an exclusion criterion for Q19 to Q27
         b) determine a frequency distribution for the percent of
            this cohort reporting a possible exposure

Q19.   -Answer will provide a frequency distribution pn the number
       of meals consumed containing Great Lakes fish.  Participants
       will then be stratified on this variable to determine odds
       ratios for Q67 to Q118, and Q119 to Q140.

Q20:.   Answer will be used to determine the relative frequency of
       consumption for each type of fish from the Great Lakes.
       Participants will be stratified according to specific fish
       species consumed.

Q21.   A frequency distribution will be compiled.  This variable
       will then be stratified and used as a criterion for grouping
       participants by years of possible exposure for comparison
       against Q67 to Q118, and Q119 to Q149.

Q22.   Answer will provide a frequency distribution on the number
       of meals consumed containing non-Great Lakes fish.  Partici-
       pants will then be stratified on this variable for determining
       ratios for Q67 to-Q118, and Q119 to Q140.  The answer will
       pertain to inland water fish meals as opposed to Great Lakes.
       in addition, this variable will be used to determine total
       potential exposure via fish consumption from any source.

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Q23.   Answer will be used to determine the relative frequency of
       consumption for each type of fish from inland waters.  Par-
       ticipants will be stratified according to specific fish species
       consumed.  In addition, this variable will be used to determine
       total consumption of specific fish species from any source.

Q24.   A frequency distribution will be compiled.  This variable will
       then be stratified and used as a criterion for grouping par-
       ticipants by years of possible exposure for comparison against
       Q67 to Q118, and Q119 to Q140.  In addition, this variable
       will be used to determine total potential exposure via fish
       consumption.

Q25.   Frequency counts will be made separately for wives, sons, and
       daughters.  Stratifications performed in Q19 and Q22 will be
       compared with the three frequency counts to determine potential
       family member exposures via fish consumption.

Q26..   A frequency distribution will be calculated for the entire
       cohort.  Reliability will be determined by comparisons with
       Q19 and Q22.  Subjects will then be stratified by this vari-
       agle for morbidity analysis with Q67 to Q118, and Q119 to Q140.

Q27.   A frequency distribution will be calculated for the entire
       cohort as well as for the various levels of stratification in
       Q20 and Q23.

Q28.   Answer will be used to r-^tablish a frequency count.  Risk
       ratios will be calculat^-n regarding the presence or absence
       of this variable and the orevalence of a morbid condition,
       listed in Q67 to Q118, and Q119 to Q140.

Q29.   This answer will be used to determine years of exposure to
       cigarettes  (i.e., smoking history).. A frequency distribution
       will be calculated.

Q30.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding high, medium, and low levels
       of consumption and the prevalence of a morbid condition listed
       in Q67 to Q118, and Q119 to Q140.

Q31.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the number of years smoked
       and the prevalence of a morbid condition listed in Q67 to^QHS,
       and Q119  to Q140.

Q32.   This question is to aid the participant in answering Q33.  A
       frequency distribution will be calculated.

Q33.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the number of years smoked
       and the prevalence of a morbid condition listed in Q67 to.QHS,
       and Q119  to Q140.

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Q34.   This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  calculated regarding  the presence or  absence  of
       this  variableJand the  prevalence  of  a -morbid condition,  Isited
       in Q67  to  Q118, and  Q119  to  Q140.

Q34a.  This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  calculated regarding  the presence or  absence  of
       this  variable and the  prevalence  of  a  morbid condition,  listed
       in Q67  to  Q118, and  Q119  to  Q140.

Q35.   This  answer will  be  used  to  determine  years of exposure  to
       pipes (i.e.,  smoking history).  A frequency of distribution'
       •will 'be calculated.

Q36.   This  answer will  be  used  to  establish  a frequency count.   Risk
       .ratios  will be  calculated regarding  high,  medium, and  low levels
       of consumption  and the prevalence of a morbid condition  listed
       in Q67' to  Q118  and Q119 to Q140.

Q37.   This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  calculated regarding  the number "of years  smoked
       and the prevalence of  a morbid  condition listed in Q67to Q118,
       and Q119 to Q140.

Q38.   This  question is  to  aid the  participant in answering Q39.  A
       frequency  distribution will  be  calculated.

.Q39,   This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  claculated regarding.the number of years  smoked
       and the prevalence of  a morbid  condition listed in Q67 to Q118,
       and Q119 to Q140.

Q40.   'This  answer will- be  used  to  establisn  a rrequency count.   RISK
       ratios  will -be  calculated -regarding  the presence or  absence of
       this  variable and the  prevalence  of  a  morbid condition,  listed
       in Q67  to  Q118,  and  Q119  to  Q140.

Q40a.  This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  calculated regarding  the presence or  absence of
       this  variable and the  prevalence  of  a  morbid condition,  listed
       in Q67  to  Q118,  and  Q119  to  Q140.

Q41.   This  answer will  be  used  to  determine  years of exposure  to cigars
        (i.e.,  smoking  history).   A  frequency  distribution will  be
       calculated.

Q42..   This  anser will be used to establish a frequency count.   Risk
       ratios  will be  calculated regarding  high,  medium, and low levels
       of consumption  and the prevalence of a morbid condition  listed
       in Q67  to  Q118,  and  Q119  to  Q140.

Q43.   This  answer will  be  used  to  establish  a frequency count.   Risk
       ratios  will be  calculated regarding  the number of years  smoked
       .and the prevalence-of  a morbid  condition listed in Q67 to Q118,
       and Q119 to Q140.

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Q44.   This question is to aid the participant in answering Q45.  A
       frequency distribution will be calculated.

Q45.   This answer will be used to establish a frequency count.  Risk
       ratios will be claculated regarding the number of years smoked
       and the prevalence of a morbid condition listed in Q67 to Q118,
       and Q119 to Q140.

Q46.   This answer will be used to establish a frequency count. . Risk
       ratios will be calculated regarding the presence or absence of
       this variable and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

Q47.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the presence or absence of
       this variable and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

Q48.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the presence or absence of
       this variable and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

Q49.   This answer will be used to establish a frequency count.  Ris.k
       ratios will be calculated regarding the presence or absence of
       this variabel and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

Q49a.  This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the presence or absence of
       this variable and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

Q50.   This answer will be used to determine years of exposure to hard
       liquor.  A frequency distribution will be calculated.

Q51.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding high, medium, and low.levels
       of consumption and .the prevalence of a morbid condition listed
       in Q67 to Q118, and Q119 to Q140.

Q52.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the number of years drunk
       and the prevalence of a morbid condition listed in Q67 to Q118>
       and Q119 to Q140.

Q53.   This question  is to aid the participant in answering Q54.  A
       frequency distribution will be calculated.

Q54.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the number of years drunk
       and the prevalence of a morbid condition listed in Q67 to Q118,
       and Q119 to Q140.

Q55.   This answer will be used to establish a frequency count.  Risk
       ratios will be calculated regarding the presence or absence of
       this variable and the prevalence of a morbid condition, listed
       in Q67 to Q118, and Q119 to Q140.

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Q56.   This  answer  will  be  used to determine years of exposure to
       beer  (i.e.,  drinking history).   A frequency distribution will
       be calculated.

Q57.   This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding' high,  medium, and low levels
       of consumption  and  the  prevalence of a morbid condition listed
       in Q67  to  Q118, -and  Q119 to Q140.

Q58.   This  answer  will  be  used to establish a frequency \count.,  Risk
       ratios  will  be  calculated regarding the number of years drunk
       and the prevalence  of a morbid  condition listed in Q67 to Q118,
       and Q119 to  Q140.

Q.6'0..   This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding the number of years smoked
       and the prevalence  of a morbid  condition listed in Q67 to Q118',
       and Q119 to  Q140.

•Q61.   This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding the presence or-absence of
       this  variable and the prevalence of a morbid condition, listed
       in Q67  to  Q118, and  Q119 to Q140.

•Q61a..  This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding the presence or absence of
       this  variable and the prevalence of a morbid condition, listed
       in Q67  to  Q  118,  and Q119 to Q140.

Q62.   This  answer  will  be  used to determine years'of exposure to
       wine  (i. e. >•  drinking history) .   A frequency•distribution will
       be calculated.

Q63.   'This  answer  will  be  used to establish a frequency count. .Risk   .
       ratios  will  be  calculated regarding high,  medium, and low levels
       of consumption  and  the  nrevalence of a morbid condition listed
       in Q67  to  Q118, and  Q119 to Q140.

Q64.   This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding the number of years drunk
       and the prevalence  of a morbid  condition listed inQ67 to Q118,
       and Q119~ to  Q140.

Q65.   This  question is  to  aid the participant in answering Q66.  A
       frequency  distribution  will be  calculated.

Q66.   This  answer  will  be  used to establish a frequency count.  Risk
       ratios  will  be  calculated regarding the number of years drunk
       and the prevalence  of a morbid  condition listed in Q67 to Q118,
       and Q119 to  Q140.

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 Q67 to Q118.
Q119 to:Q140,
        Q141,
                                                                             "A"

                                                                               2
A frequency distribution will be compiled for each disease
by the number of:
   a) Fishermen who have the disease
   b) Wives who have the disease
   c) Husband and wives how have the disease
   d) Children per family who have the disease
   e) Fishermen and one or more children who have the disease
   f) Wife and one or more children who have the disease
   g) Husband, wife, and one or more children who have the
      disease

Risk ratios of disease prevalence versus (Q18, Q19, Q20, Q21, Q22,
Q23, Q24, Q25, Q26, Q27, Q28, Q28a, Q34, Q34a, Q40, Q40a, Q49,
Q49a, Q55, Q55a, Q61, Q61a) will be calculated.
(      }   these questions will hereafter be refered to as set
Risk ratios of disease prevalence versus (Q30, Q36, Q42, Q51,
Q57, Q63, Q31, Q37, Q43, Q52, Q64, Q33, Q39, Q45, Q54, Q60, Q66)
will be calculated.
        2
(      )   these questions will hereafter be refered to as-set "B"

All information provided for these questions will be added to
the date file including validation—confirmation or denial.

A frequency distribution will be compiled for each symptom oy.
the number of:
   a) Participants who respond Yes
   b) Frequency "of occurrence
   c) Length of symptoms
   d) Participants who consulted a Doctor

Risk ratios of symptom prevalence will be calculated against
question sets "A" and "B".

All information provided for these questions will be added to
the date file including validation—confirmation or denial.

A frequency distribution will be compiled for:
   a) Number of natural children
   b) Number of step or adopted children
   c) Number of miscarriages
   d) Number of stillbirths
   e) Number of livebirths
   f) Number of other (e.g., abortions)
   g) Number of male children
   h) Number of female children
   i) Live birth weights

Risk ratios for the frequency of the above events will be
calculated against set "A" and "B".

All information "provided for these questions will be added to
the data file, including validation—confirmation or denial.

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Q141a.  A frequency distribution will be compiled for:
           a) Number of participants answering Yes
           b) Percent of deaths which are male
           c) Percent- of deaths which are female
           d) Average number of deaths for those who answer."Yes"
           e) Duration of life
           f) Causes of death

        Risk ratios for those participants who answered "Yes" to this
        question will be compared against those who answered "No" to.
        this question for set "A" and "B".

        All information provided for these questions will be added to
        the data file, including validation—confirmation or denial.

 .Q142.  A frequency distribution will be compiled for:
           a) N-umber of participants answering Yes
           b) Number of defects listed among those who. answer Yes
           c) Type of defect in self
           d) Type of' defect in sons, and percent living.
           e) Type of' de'feot in .daughters., and percent living

        Risk ratios for the frequency of the above events will be
        calculated against sets "A" and "B".

 Q143.  A frequency distribution will be calculated for the number of
        women using birth control methods.

 Q144.  The percent of women using each method will be determined.

 Q145.  A frequency distribution'of age at initiation of birth, control
        use. will be calculated.

 Q146.  The frequency distribution,of age at menarche will  be calculated.

 Q14-7..  A frequency distribution will be calculated:
           a) For the entire cohort
           b). By stratification for Q19, Q22, Q25, and Q26
           c) By stratification for set A and B questions if sufficient
              numbers of participants respond to this question.

 Q148.  A percentage rating of natural versus surgical cessation of
        menstruation will be calculated.  A frequency distribution will
        be calculated from validation reports regarding types of surgery.

 Q149.  A frequency distribution will be calculated:
           a) For the entire cohort
           b) By stratification for Q19, Q22, Q25, and Q26
           c) 3y stratification for set A and B questions if sufficient
              numbers of participants respond to this question.

 Q150.  A frequency distribution will be calculated:
           a) For the entire cohort
           b) By stratification for Q19, Q22, Q25, and Q26
           c) By stratification for set A and B questions if sufficient
              numbers of participants respond to this question.

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Q151.  A frequency distribution will be calculated:
          a) For the entire cohort
          b) By stratification for Q19, Q22, Q25, and Q26
          c) By stratification for set A and B questions if sufficient
             numbers of participants respond to this question

Q152.  A frequency distribution will be calculated:
          a) For the entire cohort
          b) By stratification for Q19, Q22, Q25, and Q26
          c) By stratification for set A and B questions if sufficient
             numbers of participants respond to this question

Q153.  A frequency count will be computed.-

Q154.  A frequency count will be computed.

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            APPENDIX VI
         Progress Reports
1. October 16, 1978 - June 15, 1979
2. April 4, 1980 (Preliminary Report)

-------
Assessment of Potential Health Risks Associated with Organic Contaminants




                  Feasibility Study - Great Lakes Basin




                             Progress Report




                     October 16, 1978 - June 15, 1979




                        Leonard M. Schuman, M.D.




                         Conrad P. Straub, Ph.D.




                        Principal Investigators



1.  Introduction




        In this progress report we summarize the activities undertaken by the




Divisions of Epidemiology and Environmental Health, School of Public Health,




University of Minnesota, Minneapolis, Minnesota, under Contract/Grant No.




EPA/R806282-01-0, during the period October 16, 1978 through June 15, 1979.




        During this period 12 students were employed to gather and assist in




the evaluation of information pertinent to the study.  These included five .




graduate students from the Division of Epidemiology under a young physician




acting as project coordinator, and seven graduate students from the Division




of Environmental Health with a scientist acting as project coordinator.




        Throughout the study period, close liaison has been maintained between




the two study groups with frequent meetings between the two project coordinators,




between the  two project coordinators and the principal investigators, and




between the  principal investigators and the total staff involved in the study.




        This progress report consists of the following sections:  1) a report




summarizing  the findings of the epidemiological study group, 2) a summary report




of the findings of the environmental study group, and 3) a series of questions




which will be the basis for the continuation of the present study during




year two.



        References have not been included with this summary statement, but will




be properly  documented in preparation of the overall project report.

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                                   -2-






        Specific directions to be taken during year two will also be defined.




2.  Epidemiologic.Studies




        A literature review was undertaken to identify epidemiologic studies




of the ..effects of organic pollutants on human health.  The two clases of




organic, pollutants of special interest were halogenated and polycyclic




aromatic hydrocarbons.  Studies of primary interest are those which associate




chronic disease effects (morbidity and mortality).with exposure to organic




pollutants.and include mutagenic, teratogenic, and/or carcinogenic effects.




Other than, the reported Yusho incident involving exposure to polychlorinated-




biphenyls (PGBs) in.-Japan, and the Michigan and Indiana studies, few




.epidemlologic studies associating PCB exposure to'human disease have been




reported.  To broaden the approach of the possible effects of PCB and other




organic pollutants on human health, reported results of animal.studies were




evaluated to provide clues for hypothesis building of effects on man.  Much




support could be found in the literature on the mutagenic, carcinogenic,




and teratogenic effects of organic pollutants on animals.  One obvious




problem is 'the translation of these results to human exposure experience.




        With the virtual nonexistence of human epidemiologic studies in  this




area, our epidemiology group explored sources which could provide basic




data .on general and cause specific mortality; infant, neonatal, and perinatal




mortality; fertility; congenital malformation, etc., for the development of




needed studies in this field.




        State health departments contacted were Minnesota, Wisconsin,




Michigan, Ohio, Indiana, and Illinois.  From  these states, rates were obtained




for infant, neonatal, and fetal deaths.  These rates were inconclusive



because of lack of adjustment for maternal' age.  From some states, congenital




anomaly rates for live births were obtained but not  for all years of interest.




It must be kept in mind that such rates are grossly  deficient because of under-




reporting, even of those anomalies detectable at birth, and because  of  the

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                                   -3-






difficulty of ascertaining many anomalies at birth.  Site specific cancer




rates by county were available from Michigan and Wisconsin; other states




reported only cancer deaths or reported deaths collectively by system, i.e.,




respiratory system, gastrointestinal  system, urinary system, etc.  Site




specific rates could probably be accessed by a review of death certificates.




This is planned.




        The National Center for Health Statistics lists cancer deaths by




system but not by site.  Only raw numbers were available.  Rates were




calculated using populations denominators from the Census Bureau.  There




were no age, race, or sex breakdowns.




        County rates were desired to compare rates for lake-bordering as




opposed to non-lake bordering counties.  Use of date currently available




for this comparison presents several problems including lack of adjustment




for urban/rural differences, different sources of water supply, differences




in length of residence, dietary habits, etc.




        From the National Cancer Institute, twenty year (1950-1969) summaries




were obtained-of site-specific cancer mortality by county.  These data were




age-adjusted using as a standard the entire 1960 U.S. population.  The data




also identified white and non-white rates.  Because the specific year data




were not available, it is not possible to examine trends of mortality over




time.  For such data, we are planning to contact the National Cancer




Institute and the National Center for Health Statistics to ascertain sex,




age, and race-specific data by county and year, and the cost thereof.




        A preliminary examination of the twenty-year data from the National




Cancer Institute indicates a possible excess of stomach, esophageal, and



other gastro-intestinal cancers in the lake-bordering counties as compared.




to non-lake bordering counties.  However, because of urban/rural differences

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                                   -4-






mentioned before, and other confounding factors, additional data are needed




before conclusions may be drawn.




3.  Environmental'Health Aspects




        Much of the available literature was reviewed, particularly that




concerned with polychlorinated biphenyl (PCB) concentrations in. air,




in water and wastewater, in sediments and soils, and in the aquatic




environment; with PBC intake by fresh water and marine organisms including




fish; with the degradation of PCBs in the various environments; and vith




the effects of exposure on animals and humans.  A general summary of this




literature review follows.




        PCS is a ubiquitous contaminant and is encountered in all environmental




media.  Some of the isozners have been identified in animal and human tissues.




The-mechanisms of transport have been indicated and do vary with the region




studied.  In the Great Lakes area, transport by air (rainfall and/or




dry deposition) is the primary source of these materials, whereas in other




locations direct discharge from industrial Or other sources may be the




major contributor.  Because these substances are generally insoluble in




water.they adsorb on to particulates and eventually deposit on'the bottom




of lakes or other bodies of water as sediment or deposit directly on the




soil.  Generally, concentrations in water are very low, in.many instances




at or just above detectable levels.  High concentrations are encountered in




sediments and extensive biomagnification occurs through the aquatic food




chain.  Volatilization and biodegradation of the less chlorinated PCBs have




reduced these levels in the sediments and have resulted in higher concentrations




of these in the air and in air particulates.  As a result, the more heavily




chlorinated compounds are retained in the sediments, taken up by micro-




organisms and retained in lipids for long periods of time.  They are not

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                                   -5—





readily degraded nor metabolized.



    Differences in results have been reported by many investigators and



these differences will have to be reconciled.  In addition, there are marked



differences in uptake among various species of organisms exposed, and there



are differences in response between so-called "pure" PCBs and those encountered



in the environment.



    In recent years attempts have been made to reduce the discharge of PCBs



from controlled sources, but these procedures have not reduced levels in the



Great Lakes or other bodies of water.  These water bodies have become sinks for



the deposition of large amounts of these substances with their retention in



sediments.  As a result, with minor degradation, they will serve as sources



for the long-term, continued contamination of organisms associated with these



environments.  Attempts must be made to identify these sources, point or non-



point, and to control them at their sites of production.  Control measures are



available for the destruction and degradation of some of these isomers.  In



other instances, more degradable isomers can be substituted for those currently



used.



    For the evaluation of possible health effects of exposure to PCB, we have



the results of the Japanese Yusho exposure incident studies, and information



on the effects of industrial exposure to PCBs during production and use to



draw upon.



    Questions have been raised as to whether the effects observed are due to



PCB itself or to the presence of certain impurities associated with these



materials.  One impurity identified in many of the PCB isomers in commercial


                                                         4      6
use is polychlorodibenzofuran, which is reported to be 10  to 10  times more



toxic than PCB.  The levels of these impurities must .be determined,



particularly since the major, route of exposure, other than occupational



exposure, appears to be related  to the consumption of fish taken from the

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                                   -6-






Great Lakes or other containingted bodies of water.  Of significance, is whether




ingestion of the mixtures of PCS isomers present in fish tissue can induce




health effects directly, as a result'of synergistic responses due to mixtures




of isomers, or related to the presence of impurities, such as the chlorinated




dibenzofurans., or other degradation products.




4.  Questions Identified as a Result of Our Studies




    From the studies carried out, numerous questions have arisen, some of which




we hope to address during the coming year.  These questions are identified




below.  In the epidemiology area the questions include:




    1.  Methods of .accessing data not directly available from existing sources:




        a.  county age-, sex-, and race- adjusted site-specific cancer




            death rates




        b.  county maternal age-, race— adjusted infant, neonatal, and




            fetal death rates




        c.  county malformation rates




        d.  county-fertility rates




    2.  Methodological problems of ascertaining trends in:




        a.  cancer mortality




        b.  infant, neonatal, and fetal mortality




        c.  malformation morbidity




        d.  fertility morbidity




    3.  Ascertainment of the appropriateness of existing methodology for




        teratogenic, mutagenic, and carcinogenic relationships to our study




    4.  Proper selection of high and low exposure contrast communities




        for retrospective studies




    5.  Possibility of doing rigourous, single community prospective studies




    6.  Appropriateness and feasibility of using bacterial and/or animal




        models to  ascertain carcinogenic, mutagenic, and teratogenic effects

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                                 -  7 -





        of the organic pollutants in lake water, j.n food chains, and in




        air at specific sites,  i.e., in an individual community




In the environmental health area the questions that have arisen include:




    1.  Significance of the polychlorodibenzofuran impurities vis a vis




        effects currently attributable to various PCB isomers




    2.  Levels of exposure to occupational workers having contact with




        PCBs, its various isomers,  or its derivatives, and health effects




        attributable to these exposures




    3.  Relevance of occupational exposures of the workers to exposure




        of his family




    4.  Determination of the sources responsible for total exposure to




        populations from environmental sources, their identity, and




        significance as exposure pathways




    5.  Identification of specific methodologies that can be used to reduce




        and/or control of the levels of PCB released to the environment




    6.  Evaluation of the methodologies used to collect samples, their




        effectiveness for providing information on actual environmental




        levels, and possible development of standardized procedures




    7.  Crititcal examination of reported data from a statistical point




        of view to assess the value of the findings reported




    8.  Determination of whether chronic toxicity is related to the




        metabolism of PCB and its intermediates or to the highly




        chlorinated stored PCBs




    9.  Study of the individual congeners, both those metabolized and




        those stored by man, is urgent because of their demonstrated




        carcinogenic potential




   10.  Evaluation of the long-term effect of release of PCBs from stored




        bottom sediment on the biota of these aquatic systems

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                                  - 8 -






    It is hoped that some of these questions can be addressed during year




two of the project to identify methodologies -that will permit, if possible,




a feasibility study, to relate environmental exposure levels to human effects.

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                Preliminary Report of




       Epidemiological and Environmental Data







                Contract //EPA/R806282
Assessment of Potential Health Risks Associated with




    Organic Contaminants in the Great Lakes Basin




                  Feasibility Study




               University of Minnesota




               Division of Epidemiology




          Division of Environmental Health




                    April 4, 1980

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                                   -1-






1.  Surgiary



        Several geographic areas in the Great Lakes Basin were selected



and matched to existing vital statistics data.  These areas were



identified by reported poiat-source discharge violations of individual



state effluent requirements by industries and municipal wastewater treat-



ment.plants.  Point sources are associated with local areas of environmental



contamination; however, the degree of environmental contamination (as



measured by water pollution parameters) cannot predict the rates and routes



of human exposure and ultimate health effects.  Furthermore, current



environmental data may not be completely indicative of past environmental



conditions in a local area.  However, current data may be viewed as a rough



approximation of past' conditions in situations where persistent contaminants



have been discharged to the environment (PCBs and like organic compounds).



Hence, the use of current environmental data  (1970 - 1979) to characterize



the previous 20 year period must be looked upon with great caution,



particularly when comparing it with 20 year (1950-1969) cancer mortality



rates,



    The use of point source dischargers of potentially toxic -materials



as indicators of environmental contamination  cannot entirely account for



differences in the cancer, fetal, neonatal, and infant mortality rates



observed between lake and non-lake counties.  These differences may be



associated with differences in the smoking, drinking, and dietary habits



of the populations being studied, their ethnic and socio-economic status,



and the degree of industrialization of the geographic areas in which



they  live and/or work.  For example, differences in stomach cancer mortality



rates may be associated with certain ethnic groups rather than environ-



mental pollutants.  Similarly, lung cancer differences may be far more

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                                   -2-






associated with cigarette consumption patterns than with exposure to




environmental pollutants.  Furthermore, the state vital statistics data




are not adjusted for age, sex, and race differences in each county




within each state.  Therefore, some observed differences between lake




and non-lake counties may be attributed to differences in the age, sex,




or race composition of the counties.




    It is apparent that the inadequacies of a county's epidemiological




and environmental data have made evaluation of the association between




riortality rates and environmental contaminant levels difficult.  However,




valuable experience has been gained during t.his initial accumulation




and evaluation of existing data, particularly in identifying•specific




areas to be addressed in future efforts and in the need to assess con-




founding and interacting risk factors.

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II.  Morbidity/Mortal!try Data Collection and Analysis




         An important initial step in determining-the potential health




     effects of certain ubiquitous organic pollutants in the Great Lakes




     Basin was to investigate the availability of existing morbidity and




     mortality data from various data sources.  It was apparent from the




     literature that PCBs (and like organic compounds) are potential




     carcinogenic and teratogenic agents.  Thus, the investigation focused




     on site-specific cancer mortality rates, fetal, neonatal, and infant




     death rates, and congenital anomaly rates  (which can be expressed as




     either death rates or as a percentage of live births).




         Since these types of organic pollutants are quite persistent




     in the environment and have been used.for over thirty years by various




     industries, data back to 1950 were sought.  However, the voluminous




     amount of data involved over this thirty year period for eight states




     (Minnesota, Wisconsin, Illinois, Indiana, Michigan, Ohio, Pennsylvania,




     and New York) warranted a limited examination of these data.  Specifically,




     state vital statistics were thoroughly examined for Minnesota, Wisconsin,




     Illinois, "Indiana, Michigan, and Ohio for every fifth year from 1950




     plus the. most.current year  (usually 1977).




         The hypothesis was posed that areas adjacent to the Great Lakes have




 a greater exposure potential to these pollutants than those areas more distant




 from the Lakes based on the following assumptions:




         1.  Most lake bordering communities have increased industrial




             activity compared to most non-lake bordering communities.




         2.  Individuals living  in these adjacent communities are more




             likely to be occupationally exposed to those pollutants being




             discharged into the air and the Lakes.

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    3.  Close proximity to the Lakes would lead to greater exposure




        via ambient air, soils, and water.




    A.  Fishermen living in these lake-adjacent communities are




        more likely to fish these lakes and may have higher




        exposures due to consumption of their catch.




    Based on this hypothesis, it was decided to examine (for camparative




purposes) the morbidity/mortality data for lake and non-lake communities.




Since the smallest geographical area for which data are readily




available in the country,  the investigations centered on gathering




data by county and comparing lake counties to non-lake counties




within each state.




    The first source investigated was the vital statistics record for




each individual state.  Rates by county for site-specific cancer




mortality; fetal, neonatal, and infant mortality; and congenital




anomalies were requested from each State vital statistics office for




the following years:  1950, 1955, 1960, 1965, 1970, 1975, and 1977.




The data available are indicated below:




    1.  All states have data available by county on fetal, neonatal,




        and infant death rates.  However, in most cases, these are




        not race or sex specific.




    2.  All states have rates for deaths due to congenital anomalies by




        county but these are not age, sex, or race specific.  Data on




        congenital anomalies as a percent of live births by county

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                                   —5—






        are available from selected states for selected years.  These data,




        again, are -not sex or race specific.




    3.  Most states do not have site-specific cancer mortality rates by




        county.  For the states that do, the data are generally not age-




        race- or sex-specific.








    (For a.more detailed account of the data available from each state,




     refer to the cover page which precedes each individual state's




     vital statistics).




    The yearly publications of the National Center for Health Statistics




(NCHS) were also examined for relevant information.  These data are




comparable to the data obtained from each individual state.  The NCHS




does publish fetal, neonatal, and infant death rates by county but they




are not race-or sex-specific.  They also publish death rates for congenital




anomalies by county-.  Site-specific cancer mortality data are not published




by county by the NCHS.




    The best source of cancer mortality data by county was found in the




National Cancer Institute publication entitled:  "U.S.. Cancer Mortality:




1950 - 1969*'.  This publication contains 20-year summary rates of site-




specific cancer mortality by county.  These rates are sex-and race-specific




and age-adjusted.  The drawbacks of these data are:  1) since the rates




are a 20-year summary, time trends cannot be examined and 2) there are




no similar data that are more recent.




    Lake bordering and non-lake bordering counties were compared using the




following information:  1) site-specific cancer mortality rates from the




NCI 20-year data and from state vital statistics and 2} fetal, neonatal,




and infant death ratios from state vital statistics data.  The fertility




and congenital malformation rates were also compared when these types of

-------
                                   -6-






    data were available from the state's vital statistics.




    Mean values for all cancer death rates and fetal, neonatal, and infant




death rates of lake-bordering counties were compared to the respective




mean values of non-lake bordering counties within each state.  Those




parameters having higher mean values in lake-bordering counties than non-




lake bordering counties are noted for each state, (see cover sheet attached




to state data).  No tests of statistical significance were calculated for




these observed differences.




    Maps were prepared for each state depicting the counties with the highest




and lowest rates (within that particular state) for selected parameters.




The parameters used included the NCI 20-year cancer mortality data for




cancers of the stomach, lung, esophagus and all neoplasms.  These particular




cancer sites were chosen because their mean mortality rates appeared to be




consistently higher for lake-bordering counties than for non-lake bordering




counties in all states.  Additional parameters include the fetal and neonatal




death rates for the years 1970, 1975, 1977, percent of live births with




congenital anomalies for the years 1970, 1975, 1977, (only available for




Wisconsin, Michigan, and Minnesota) and fertility rates for the years 1960,




1970 (only available for Ohio, Wisconsin, and Minnesota).  The means and




standard deviations were calculated for all available parameters within




each state.  Those counties with rates significantly Qf 2S.D.)




different from the state mean are identified with an asterisk on the mans.

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                                       —7—






III.  Selection of Study Populations Based uponJSnyironmental Factors




           Upon completion of an extensive scientific literature review of




      PCBs (and like organic compounds), it was apparent that these substances




      are ubiquitous environmental contaminants.  Much of the data, however,




      clearly demonstrate that there are regional differences and that the




      effects are consistent across all media (e.g., water, sediments, fish,




      and birds), generally showing greater concentrations in highly developed




      areas and areas of industrial activity.  Thus, it was not surprising




      that the Great Lakes Basin area (the most industrialized area in the




      United States), based on the literature review, appeared to be experiencing




      a higher degree of environmental contamination with these potentially




      toxic materials as compared to other regions of the country.




           To elaborate further on the degree of environmental contamination




      the Great Lakes Basin has sustained from PCBs (and like organic compounds),




      it was quite obvious that our data base had to be expanded to include




      specific quantitative and qualitative data and information of specific




      areas within the Basin.  Thus, various state, federal, and international




      environmental agencies (with some type of jurisdiction in the Basin) were




      consulted concerning the existence and availability of specific




      environmental data.  Each agency was requested to provide qualitative and




      quantitative data on organic contaminants which the agency -had identified




      in point sources (waste water discharges), atmospheric sources, waste-




      water sludges, runoff, sediments, ambient water, drinking water, landfill




      leachates and vent gases, and fish caught within the Basin.




           The agencies and organizations contacted responded favorably to




      the request but little meaningful data were actually received.  Generally

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                                   -8-
it was apparent that considerable environmental data do exist but are




not readily available for review or compilation.  (Most states store such




data in vertical files.)  However, a series of documents were received




from the International Joint Commission (IJC) which were compilations of




data solicited from various Canadian and American jurisdictions within the




Great Lakes Basin (1,2,3,4).  It should be noted that the IJC documents




include data that some state officials have identified as also being




available from their agencies; therefore, there may be duplications of




data when multiple data sources are used.




    The IJC documents were thoroughly reviewed and it was decided that the




data contained therein could be used to select (on the basis of various




criteria) specific areas or communities within the Basin that may be




experiencing a high degree of PCS (or like organic compound) contamination.




It was then assumed that people living in these communities could have




potentially higher degrees of exposure to these contaminants in their




immediate environment.  Thus, this attempt permitted an initial approach




at integrating environmental data which identified groups 'receiving potentially




higher exposures with morbidity/mortality data.  A number of approaches were




taken and will be discussed.




    The focus of this investigation is to identify study populations which can




be used to evaluate the potential health risks associated with organic




contaminants in the environment of the Great Lakes Basin.  Using data fron




the International Joint Commission Great Lakes Water Quality Board concerning




designated "problem areas", high PCB concentrations in Great Lakes sediments,




and point source dischargers, several communities were selected for further




environmental and epidemiological characterization.



    Table 1 lists "problem areas" based on an evaluation of the data

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                                   -9-





contained in the "Great Lakes Water Quality 1978 Report".  These "problem



areas" were selected because of .reported violations of water quality



standard with respect .to organics (i.e., .phenols, chlorobenzenes) and/or.



PC3s.  Point sources were implicated as the cause of these problem areas



but were not recorded in this table.



    Other communities were selected as potential sites' for.environmental



and epidemiological evaluation using existing sediment data from the "Status



Report on Organic and Heavy Metal Contaminants in the Lakes Erie, Michigan,


                              (2 3)
Huron, and Superior Basins".  A ' 'Table 2 lists committees adjacent to the



Great--Lakes where PCB bottom sediment concentrations' ^mg/kg have been



Identified.  These high levels are thought to be associated with point



source dischargers but were not so identified in this report.

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                                   -10-
                                  TABLE  1


     Followiag an examination of  the data  obtained  in the "Great  Lakes

 Water Quality 1978  Annual Report"  the  following geographical areas  were

 identified as possible sites for future epidemiologlcal and environmental

 evaluation.  These  identifications  were based upon violations of objectives

 or standards with respect to organics (i.e.,  Phenol) and/or PCBs. Both

 industries and wastewater treatment plants  are identified as being

 potential sources for these discharged  substances.   The following table lists

 the problem areas and the associated lake.



 LAKE                                 PROBLEM AREA

 Superior                             Thunder Bay,  Jackfish Bay **

 Huron                                St.  Marys River,  Spanish River  **

 Michigan                             Waukegan Harbor,  Indiana Harbor

 Erie and St. Clair                    St.  Glair River,  Detroit River, Cleveland
                                      area,* Black  River, Rouge River, and
                                      Ecorse River

 Ontario                              Buffalo River, Upper Niagra River, Lower
                                      Niagara River, Mississauga  - Clarkson area,  **
                                      Grass  River
 *Areas where water quality objectives  have not been achieved  because  remedial
  programs are not yet completed.

**Canadian jurisdiction

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                                   -11-
                                 Table.2

List .of. communities adjacent  to  the Great Lakes where PCB* concentrations

equal to or greater than  1 mg/kg have been  identified in  the bottom

sediments.
Lake Basin
   Location
   PCB, mg/kg
     Level
"Michigan
Erie
Huron
Superior
Ontario
Waukegan, IL

Indiana Harbor, IN

Fox River, WI

Escanaba, MI

Manistique, KE

Milwaukee, WT

Cuyahoga River, OH

Ashtabula, OH

Fairport, OH

Cleveland, OH

No data presented

 above 1 mg/kg.

No data presented

 above 1 mg/kg.

Hamilton, ONT.

United States locations

were below 1 mg/kg
 0.1 to 16,400

 .04 to 25.7

0.67 to 11.56

     1.6.

10.2, 3.2, 25.5H

     6.4

 0.29 to 2.20

<0.1 'to .1.10

  0.7 to 1.10.

<0.01 to 2.30
 1.3 to 10
+  Combined Aroclor  1254 + 1242

*  Total  PCB

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                                   -12-






    Point source dischargers can account for higher local levels of



contaminants as compared to those levels found in the surrounding environment.



These sources may influence the exposure potential and effect the health



status of surrounding communities.  Point sources include combined storm



sewers, wastewater treatment plants, industries, and power plants.  The



data contained in an "Inventory of Major Municipal and Industrial Point



Source Dischargers in the Great Lakes Basin" were used to identify sources


                                                                    (4)
of potentially hazardous organic compounds in the Great Lakes Basin,    and



select and compare several geographic   areas or possible sites for a



cooperative epidemlologic and environmental study.  The areas selected are



identified based on the following criteria:



    1.  communities in which at least one industry measured organics (i.e.,



        phenols) prior to discharge and complied with state- effluent



        requirements, and in which the wastewater .treatment plants complied



        with state effluent requirements [on the basis of phosphorus (P),



        biological oxygen demand (BOD), and suspended solids (SS)]. (Tbl 3)



    2.  communities in which at least one industry measured organics (i.e.,



        phenols) prior to discharge and failed to comply with state effluent



        requirements, and in which a wastewater treatment plant failed to



        comply with state effluent requirements (on the basis of P, BOD,



        and SS).  Table 4.



    3.  communities in which at least one industry measured organics (i.e.,



        phenols) prior to discharge and failed to comply with state effluent



        requirements, and in which the wastewater treatment plants complied



        with state effluent requirements (on the basis of P, BOD, and SS).



        Table 5.

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                              -13-






4.  communities in which at least one industry measured organics




    (i.e., phenols) prior to discharge and complied with, state effluent




    requirements,  and in which a wastewater treatment plant failed to




    comply with state effluent requirements (on the basis of P, BOD,




    and SS).  Table 6.

-------
                                  -14-
                                 TABLE 3

    Communities in which at least one industry measured organics (i.e.., phenols)

prior to discharge and complied with state effluent requirements, and in which

the wastewater treatment plants complied with state effluent requirements

(on the basis of P, BOD, and/or SS).
Allen
Porter

Gratiot
Saginaw
St. Clair

Erie
Niagara
S t. Lawrenc
S t. Lawrenc:
City


Fort Wayne, Ind.
Chesterton, Ind.

Alma, Mich.
Saginaw, Mich.
Port Huron, Mich.

Lackawanna, NY
N. Tonawanda, NY
Messena, NY
Ogdensburg, NY
                                                  Cancer Mortalitv Rates
high esophagus (6.7), stonach
(18.8), lung (47.8) all neo-
plasms (207.0)

high esophagus (5.5)
* Identified as being high from preliminary reported information for a
  a particular county in a specific state.

Qlndicates mortality rates (deaths/100,000) within a specific county
  for respective states.

-------
                                  -15-
                                 TABLE  4

    Communities in which at least one industry measured  organics  (i.e.  phenols,)

prior to discharge and failed to comply with  state effluent requirements,

arid in which a wastewater treatment failed  to comply with  state effluent

requirements (on the basis of P, BOD, and/or  SS).
                          City                       Cancer Mortality  Rates

                          E. Chicago, Ind.           high esophagus  (5.4), stomach
                                                     (21.9),  lung  (47.9), all
                                                     neoplasms  (198.4)

Kalamazoo                 Kalamazoo, Mich.
Ottawa                    Holland, Mich.
Wayne                     Wyandotte, Mich.           high esophagus  (6.4), lung
                                                     (47.3),  all neoplasms  (209.2)

Allen                     Lima, OH
Cuyahoga                  Cleveland, OH              high esophagus  (7.5), stomach
                                                     (20.7) lung (45.8), all
                                                     neoplasms  (211.9)
Lorain.                    Lorain, OH                 high esophagus  (5.6), stomach
                                                     (20.2),  all neoplasms  (189.7)
*See Table 1

-------
                                   -16-
                                 TABLE 5




     Communities in which at least one industry measured organics




(i.e., phenols) prior to discharge and failed to comply with State




effluent requirements and in which the wastewater treatment plants




complied with state effluent requirements (on the basis of P, BOD,




and/or SS).











County             City                  Cancer Mortality Rate*




Niagara            Lockport, NY          high esophagus (5.5)
*See Table 1

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                                 TABLE 6

     Cocsaunities. in whicn at least one industry measured organics

(i.e.;.phenols) prior to discharge and complied with State effluent re-

quirements, and in which a wastewater treatment plant failed to comply-

with State, effluent requirements (on the basis of P, BOD and/or SS)..
lake
Wayne.


Erie



Monroe

Niagara


Cuyahoga


Lucas


 *See Table 1
**Areas where
     City

     E.  Chicago,  IL



     Gary,  IN
Alpena
Bay
Gene ss e
Ottawa
Wayne
Alpena, MI
Bay City, MI
Flint, MI
Holland, MI
Detroit, MI
     Trenton, MI**


     Tonawanda, NY**



     Rochester, NY

     Niagara Falls, NY


     Cleveland, OH**


     Toledo, OH
Cancer Mortality Rate*

high esophagus (5.A), stomach
(21.9) lung (47.9), all
neoplasms (198.A)

high esophagus (5.4), stomach
(21.9), lung (47.9) all
neoplasms (198.4).
                                         high lung (43.9), all neoplasms
                                         (196.4)
high esophagus (6.4), lung (47.3),
all neoplasms (209.2)

high esophagus (6.4), lung (47.3),
all neoplasms (209.2)

high esophagus (6.7), stomach
(18.8), lung (47.9), all neoplasms
(207.0)
high esophagus (5.5)
high stomach (21.7)

high esophagus (7.5), lung (45.8)
stomach (20.7), all neoplasms (211.9)

high esophagus (5.7), lung (45.6),
all neoplasms (196.4)
there are 2 or more WTPS, some in compliance others not.

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                                     -18-






     Following preparation of these city lists, the state maps




indicating high and low county cancer mortality rates were used to




determine if these cities were located in counties with unusually




high cancer mortality rates.  Blanks for cancer mortality rates do




not imply an absence of cancer, but that the rate for the county




containing the specific community was not among the five highest in




the state for any of the cancer sites of esophagus, stomach, and




lung and/or all neoplasms.  These results indicate a possible associ-




ation between point sources of hazardous materials, their compliance




status with respect to state effluent requirements, and cancer mortality




rates.




     It was hypothesized that those counties with point-source dis-




chargers of hazardous materials in- compliance with state effluent re-




quirements may have different cancer rates than those counties with




point source dischargers of hazardous materials failing to comply




with state effluent requirement.  A two tailed T-test was used to




evaluate the level of significance for differences observed between




cancer mortality rates in counties containing cities listed in Table 3




(i.e., communities in which at least one industry measured organics




prior to discharge and complied with state effluent requirements and




in which the wastewater treatment plants complied with state effluent




requirements) and counties containing cities listed in Table 4 (i.e.,




communities, in which at least one industry measured organic prior to




discharge and failed to comply with state effluent requirements and




in which a wastewater treatment plant failed to comply with state




effluent requirements).




     The mean cancer rates fox counties listed in Table 3 (total com-




pliance) were compared to the mean cancer rates for counties listed

-------
                                   -19-


in-Table 4 (total non-compliance).  The cancer mortality rates for

counties containing the cities listed in Table 4 were significantly

greater than the cancer rates in counties containing cities listed

in Table 3 at the p•» 0.05 level for the following sites:



             white males             stomach (0.012)*

                                     kidney (0.029)

         non-white males             nose, auxiliary sinuses, etc.

                                     (0.011)



and at the p.« 0*10 level for the following sites:



             white males             nasopharynx (0.080)

                                     pancreas    (0.075)

                                     all other   (0.092)

           white females             stomach     (0.097)

                                     pancreas    (0.098)

         non-white males             stomach     (0.100)



     However, the cancer mortality rates for counties containing the

cities listed in Table 3 (total compliance) were significantly greater

than the cancer rates in counties containing cities listed in Table 4

(total non-compliance) at the p = 0.05 level for the following sites:
             white males             nose, auziliary sinuses, etc.
                                     (0.041)

           white females             nose, auziliary sinuses, etc.
                                     (0.032)
*() Indicates p value

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                                   -20-






             non-white males         salivary        (0.049)




                                     malanoma        (0.032)




                                     lymphoma, etc.  (0.030)




                                     all other       (0.013)








and at the p * 0.10 level for the following sites:








              non-white males        biliary and liver   (0.059)




            non-white females        salivary            (0.099)




                                     biliary and liver   (0.099)




                                     other skin          (0.059)

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                                   -21-
                               Bibliography








1.  "Great Lakes Water Quality 1978 Annual Report."  International




    Joint Conmission (IJC), Great Lakes Water Quality Board,  July 1979.








2,  "Great Lakes Water Quali.ty - Appendix E:  Status Report on Organic




    and Heavy Metal Contaminants in the Lakes. Erie, Michigan, Huron,




    and Superior Basins."  IJC, Great Lakes Water Quality Board,




    July 1973.








3*  "Great Lakes Water Quality - Appendix E:  Status Report on the




    Persistent Toxic Pollutants in the Lake Ontario Basin."  IJC,




    Great Lakes Water Quality Board, December 1976.








4.  "Inventory of Major Municipal and Industrial Point Source. Dis-




    chargers in the Great Lakes Basin."  IJC, Great Lakes Water Quality




    Board, July 1978.

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                                   -22-






IV.  Intense Environmental Characterization:  Pilot Study to Determine




     the Availability of Existing Environmental Data






          It was apparent that the preliminary selection of potential




     study counties was based on very broad and non-specific data.




     However, these counties were selected on the premise that detailed




     quantitative and qualitative data on specific contaminant concen-




     trations in various environmental strata could be obtained and




     evaluated.  Thus, a complete environmental characterization




     could be developed for each individual lake county, with the




     intent of ultimately determining morbidity and mortality rates




     and potential routes of exposure of the indigenous populations




     to specific contaminants.  A similar approach could be taken




     •for counties chosen as controls (i.e., non-lake counties).




          In order to accomplish these objectives, a pilot study to




     determine the availability of existing data and to evaluate the




     usefulness of these data in assessing possible exposure levels




     was established.  Two counties were selected within the same




     state to determine the kinds of data available locally.  Additional




     data were obtained by contacting State and Federal agencies who




     were responsible for environmental monitoring in these counties.




     These data would provide insight on some aspects of the prevailing




     environmental conditions.  Furthermore, deficiencies in the




     available data could be identified and the need for additional




     monitoring could be evaluated.




          The state environmental agency was contacted first, to request




     access to their file's, which contained environmental information




     for these counties.  The following were requested:  1)  surface

-------
                              -23-






water dischargers (wastewater):  NPDES permits, effluent and




sludge analysis, for specific potentially toxic materials (PCBs),




sludge handling and disposal practices, and sludge disposal sites-




2)  public drinking water supplies:  source (ground or surface),




treatment provided, general water quality parameters, and specific




analyses for potentially toxic materials (PCBs, chlorinated




pesticides) of both the finished and raw water; 3)  sanitary




landfills:  location, leachate analysis, types of materials




disposed, and vent gas analysis; 4)  ambient air:  location of




monitoring stations, specific parameters, and location of in-




cinerators;. 5)  special environmental monitoring surveys for




PCBs, pesticides, etc.:  lake and river sediments, ambient water,




fish flesh, and soil.  Other agencies in the state were also




contacted and relevant data were requested.  These include:




flesh analysis of fish caught within the waters of each county,




and market-basket analysis of food products consumed.




     The USEPA, Revion V office was contacted for data pertaining




to environmental concentrations of organic contaminants in these




counties available through their computer information service




(STORED.




     Studies conducted by NIOSH evaluating the health hazards




associated with PCB usage in industry were requested to evaluate




potential worker exposure in similar industries located in the




study counties.




     The following summary characterizes the types of data col-




lected from these data sources.



1.  State environmental agency data and information.

-------
                         -24-






a.  Agenny personnel have identified as suspect seven land-




    fills in one county and two in the other .as receiving




    PCBs (wastewater treatment sludges and industrial oils




    and solvents).  The monitoring of leachates and aquifers




    beneath landfill sites will be initiated in 1980; very




    few specific results are available at this time.




b.  27 wastewater dischargers (i.e., industrial and municipal




    wastewater treatment plants - WTPs) in one county and




    four in the other were identified as potential sources




    of PCBs or other similar synthetic organic compounds.




         Two large WTPs in one county reported detectable




    levels of specific synthetic organic compounds (PCB




    and DDT) and a phthalate ester in their effluents.




c.  Public water supply information.indicated that six areas




    in one county obtain drinking water from surface supplies.




    The remainder of the county population obtains drinking




    water from groundwater sources.  In the other county




    drinking water is obtained exclusively from ground sources.




         Pesticide levels in surface water supplies are




    available for one county.  These date are pursuant to




    the provisions of the safe drinking water act.  However,




    they are reported as meeting the requirements rather




    than reporting the actual concentrations found.  No




    PCB data exist for groundwater supplies.




d.  Surface water quality data are available for one county




    and included measurements for dieldrin, DDT (total),




    and PCBs in three locations (two rivers and an adjacent

-------
                              -25-






         lake).  Surface water quality data for pesticides or




         PCBs are not available for the other county




     e.  Two environmental surveys have been carried out in one




         of the counties.  These included analyses of fish,




         ambient water, and sediments for PCBs and other synthetic




         organic compounds.  No such surveys were performed in




         the other county.




     f.  Fish analyses in the one county included PCB,  and




         chlorinated pesticides concentrations in lake  trout and




         chinook salmon.  No data were reported for the other




         county on PCBs or ai\y other contaminants.




2.  USEPA "STOKET" computer information




     a.  Surface water quality data are available for both counties;




         these data do not include measurements for PCBs, pesticides




         or other organics.  However, phenolic and oil  and grease




         concentrations are measured at several monitoring




         stations in both counties.




     b.  Data on analysis of sediments are sparse.  There are only




         two monitoring stations in one county, and four in the




         other.  Results are available from analysis of only one




         sample collected at each monitoring station.




     c.  Analysis of fish collected in both counties were available.




         Data are not, however, consistent throughout the computer




         print-out.  Critical variables not reported include:




         species, age, sex, weight, time of year tested, portion




         tested and fat content.




     d.  There is no information on PCB (or other synthetic

-------
                              -26-






         organic compounds) concentrations in air, landfill




         leachates or vent gases.




3.  State Departments of Conservation and Public Health information.




     a.  Both agencies have generated copious quantities of data




         pertaining to fish flesh analyses for PCBs, heavy metals,




         and chlorinated pesticides.  A large variability exists,




         however, in the time of year samples, number per sample,




         and type of sample (i.e., whole, fillet, etc.).  For one




         county data are available for yellow perch, lake trout,




         coho salmon, and bloaters.  In the other county, only




         carp were surveyed consistently for PCBs and chlorinated




         pesticides.




4.  Local health departments were contacted but their data were




    similar, if not identical, to data obtained from the state




    agencies.




5.  Research data from universities in the vicinity of the counties




    were contacted.  However, to date no data have been obtained




    from these sources.




     The location of specific data sources are plotted on large-




scale county maps.  These are useful in locating dischargers of




various contaminants and their potential for the contamination of




fish, water, sediments, etc. and the location of possible sources




of exposure to local population groups.  It should be pointed




out that these data, for the most part, covered concentrations




measured and found in the last five to ten years.  However, since




these contaminants (PCBs and chlorinated organic pesticides)




are highly persistent, and have been in the environment at various

-------
                              -27-






levels since their industrial use, it may be possible to extrapo-




late these findings over a longer period of past exposure.  Finally,




when specific study counties are eventually selected the experience




learned in this pilot study should provide a useful approach to




the characterization of study county environmenta.

-------