LAKE ERIE WATERSHED
STUDY PLAN
PRELIMINARY
March 19&3
U. S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE
Public Health Service
Division of Water Supply and Pollution Control
Great Lakes-Illinois River Basins Project

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TABLE OF CONTENTS
Page
INTRODUCTION	1
OBJECTIVES	2
BASIC DATA COLLECTION	3
Water Uses	3
Water-borne Wastes	3
Water Quality-Appraisal	3
Geology	4
Horology	4
FIELD OPERATIONS	5
Sampling Program	5
Industrial Outlets	5
Stream Sampling	5
Lake Erie Sampling	6
Flow Measurements	6
Lake Currents	7
Special Studies	8
IABORATOKY PROGRAM	12
Biology	12
Chemistry	14
Bacteriology	15
Radiochemistry	18
-PLAIT FORMULATION	19
Engineering "Analyses	19
Economic and ]>_mr>graphir-	19
Special Studies	21
FIELD STATION ORGANIZATION	22
TIME SCHEDULE	23
i

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INDEX OF FIGURES
Following
Figure No.	Page No.
1	Lake Erie Basin	1
2	General Location of Stream
Sampling and Inshore Sampling	5
Stations
3	Deep Water Sampling. Stations
and Cruises	6
4	Current Meter Locations	7
5	Organization Chart	22
6	Lake Erie Work Schedule	23
ii

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1
INTRODUCTION
The Department of Health, Education, and Welfare, Public
Health Service, Great Lakes-Illinois River Basins Project, in
cooperation with State agencies concerned with public health,
water pollution control, water resource development, and related
fields, and with other Federal and Interstate agencies, is
planning to undertake a study of Lake Erie beginning the first
quarter of fiscal year 1963. The study will be undertaken as a
part of the Public Health Service's activity to develop compre-
hensive programs for water quality management for interstate
waters and tributaries thereof. Similar studies are currently
being carried out on the Illinois River and Lake Michigan; future
studies are planned for the remaining Great Lakes. See figure
No. 1. It is intended that a comprehensive program for water
quality management for the entire Great Lakes Basin will be
available in 1968.
The study will be undertaken in cooperation with State
and local Water Pollution Control agencies, the municipalities
and industries in the study area, and with other Federal and
Interstate agencies concerned. In the development of the com-
prehensive plan, due regard will be given to the improvements
and water resource management practices which are necessary to
conserve the water quality in Lake Erie and tributaries for
public water supplies, propagation of fish and wildlife, recre-
ational purposes, agricultural, industrial, and other legitimate
uses.
Preliminary studies will begin shortly after August 1,
1962, to be followed by an intensive program of sampling,
laboratory analysis and measurement of currents to be undertaken
on or about July 1, 1963- The preliminary studies will be
limited to a review of prior water quality studies of Lake Erie
and tributaries, the development of an inventory of water
supplies and sources of waste water, a study of water uses and
water resource management practices, preliminary economic and
demographic studies, and hyrological and meteorological studies.
The Project will rely on contractual services to supplement the
activities of the Project in certain fields of study. Plans
are to establish a field office in Cleveland shortly after
July, 1962, and a field laboratory will be established on or
about July 1963 in anticipation of the intensive studies to be
undertaken in Lake Erie after July, 19^3•

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2
OBJECTIVES
The objectives of the study are:
1.	To determine the present quality of the waters of
Lake Erie and its tributaries, "by a review of existing records
supplemented by a field sampling program.
2.	To inventory water uses and trends of usage in the
Basin, for municipal and industrial water supply, recreation,
aquatic and wildlife, navigation, agriculture, and other purposes.
3.	To compile data on sources of pollutional quantities
and characteristics of water-borne wastes, in each sub-drainage
basin, catalogued by sources and points of discharge.
4.	To appraise the adequacy of present practices in
land use, water use, and waste disposal in relation to water
quality protection.
5.	To measure Lake Erie currents and establish their
patterns as an aid to predicting the fate of pollutants discharged
into the lake and resultant effects on the lake.
6.	To make economic and demographic studies leading to
projections of future growth, accompanying water demands, and
associated water-borne wastes.
7.	To determine the existing and potentially developable
water resources, ground and surface, within the Basin.
8.	To formulate a plan for guidance of a cooperative
program aimed at improving and protecting the quality of waters
in the Basin. This includes development of agreements on the
water quality goals, analysis of measures for achieving these
goals, and time tables for their accomplishment.

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BASIC DATA COLLECTION
3
The collection of "basic data will be one of the first
activities started, and will "be a continuing activity. Contacts
will be established and maintained with persons and agencies in
the area having pertinent information and records. First efforts
will be directed toward determining what information is available,
the gaps in needed information, and how these gaps may be filled,
either by Project investigations or cooperative effort by others.
Discussion of specific categories of basic data follows.
Water Uses
The inventory of municipal and industrial water uses in
the Lake Erie Basin will be compiled for each sub-drainage basin.
The published data will be updated where needed through state
office visits for collecting data from unpublished reports.
In addition to the municipal and industrial water uses,
data will be collected and analyzed to show all water uses,
including agricultural, recreational, fish and aquatic life
and wildlife, and other legitimate uses.
All data will be cataloged in appropriate and meaningful
units.
Water Borne Wastes
A comprehensive picture of the wastes loadings to receiving
waters in the basin will be obtained. The state agencies and
other agencies will be asked to cooperate in obtaining the data
via state records, plant visits and/or plant sampling at selected
plant sites, both industrial and municipal. Where practical,
information will be obtained relative to the loadings contributed
by shipboard discharges and dredging operations in Lake Erie and
tributary streams.
Water Quality Appraisal
Existing reports will be used as a source of data for
determining the present water quality and as a guide in deter-
mining where stream sampling is needed.

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b
Geology
A geological study of the Basin will supply necessary
¦background material for determining availability of ground
water supplies for local communities and industry. Geological
publications and available engineering reports are expected to
yield valuable data for this purpose.
Hydrology
A water budget study of Lake Erie will be required to
determine the effects of the various sources of pollution on
the present and future water quality of the lake. This study
would include determination of Lake Erie water supply and
water loss. Supply includes rainfall, inflow from Lake Huron,
inflows from tributary streams, and groundwater inflow. Out-
flow includes evaporation and outflow to Lake Ontario. The
determination of evaporation may require collection and analysis
of considerable meteorological data.
Low-flow frequency studies will be required on tributary
streams to determine flows available for water supply and
quality control purposes. This would include a survey of past
data with appropriate projections to cover the circumstances
found in the study. Sources utilized would include published
Weather Bureau data, Corps of Engineers and Lake Survey data,
and the publications of the U. S. Geological Survey. Flow
data from tributary streams may involve, in some instances,
the establishment of supplemental stations for stream gaging,
probably with the cooperation of the U. S. Geological Survey
on a contract basis. Flows from individual industries, treat-
ment plants, and municipal sources or drains should be obtained
to complete the flow balance and find what specific contribution
is being made to the pollution budget of the stream.

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FIELD OPERATIONS
5
Sampling Program
Field activities will provide for the actual collection
of the routine and special samples. This would entail coordina-
ting car routes, industrial outlet sampling, bridge sampling,
boat sampling, couriers, transportation of samples and the
selection and training of personnel. The scope of the sampling
program involves the maintenance and operation of sampling
stations at industrial plants, on Lake Erie, and selected
tributaries, plus the maintenance and operation of monitoring
equipment such as dissolved oxygen analyzers and carbon filter
stations.
Industrial Outlets
It is anticipated that the Study Plan will require the
sampling of waste water outlets from industrial waste discharges
discharging separately to the water courses under study. Sampling
at industrial outlets will involve measurements of flow through
the use of current meters, weirs, or automatic devices depending
upon the amounts of flow, accessibility, etc. Sample collection
must be made either in proportion to the flow, or flow data must
be secured and submitted along with constant volume samples.
This will permit compositing the samples in proportion to flows
at sampling time. Industrial waste sampling would include the
installation and maintenance of flow gages, weirs, pumps, pro-
portional sampling devices, conductivity and pH measuring equip-
ment, filter installations (industrial), and other sampling and
monitoring equipment as required by the waste being sampled.
Stream Sampling
Sampling of the major tributaries will be carried out,
and limited sampling of the minor tributaries. Figure No. 2
shows the location of proposed major tributaries for sampling
to determine their relative importance in the overall water
quality picture.
The following are major streams proposed for sampling:
Huron River
Raisin River
Ifeumee River
Sandusky River
Black River
Cuyahoga River
Cattaraugus Creek
Buffalo River
Niagara River

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Samples will be taken a reasonable distance upstream from the
inlet to determine the quality of the tributary and a radial
sampling pattern will be conducted at the inlet to define the
area of dispersion and degree of dilution. The specific
location and exact number of the sampling stations upstream
from the mouth will be decided after field reconnaissance
studies and review of existing data.
Lake Erie Sampling
Deep Water. A deep water sampling study will be instituted
to determine the water quality of the lake and its variation
with depth. The station locations will be the same as that
of lake current metering network for the purpose of constructing
lake profiles and tracing the pattern of movement of these
profiles. Utilization of oceanographic boats will be made when
possible. Figure No. 3 shows the proposed cruise for the U. S.
waters and its Canadian counterpart.
Inshore. In addition to the radial pattern of sampling where
tributaries empty into Lake Erie, samples will be collected
from inshore waters within the vicinity of municipalities and
other areas discharging large quantities of wastes. See Figure 2.
The inshore sampling program will furnish valuable data for
determining the effective influence of waste outfalls and
tributaries on the lake water quality. This data will also
be useful in determining effects of tributary and lake currents
in the dilution of wastes and their pattern of travel.
Inshore sampling, as such, may not be necessary in areas where
tributary sampling gives the desired information.
Flow Measurements
Flow measurements would be instituted to determine the
amount of the flow at any given cross section or range where
necessary to implement the field data. The hydrometric data
would be drawn largely from the work of a hydraulics unit
supplementing published data obtainable from other governmental
agencies.

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The field work and. connected office computations could
be carried on by the engineers and engineering aides who will
also handle hydraulic measurement and instrumentation for
stormwater overflow studies as described in another section
of the Study Plan.
lake Currents
Water current studies will be carried out in Lake Erie
to determine both the gross circulation and to relate this
circulation to wind distribution. Ultimately, it is hoped
that wind data from existing meteorological land stations and
that supplemented by project investigation can be used to help
predict water currents. Knowledge of water currents is essential
for predicting transport of pollutants in the lake.
Automatically recording instrument stations will collect
water speed, direction and temperature data from several depths
for one year and surface wind velocities and temperature for
most of a year in the eastern and central basins. The location
of these stations is shown in Figure No. k. Free floating
drogues will be used to provide water current information in
the western basin which is too shallow to permit the use of
automatic current meters. Drogue information will be available
for selected daylight periods, each several hours in length,
whereas metered current information will be available on a
round the clock basis.
Meteorology
Studies to determine wind, temperature, heat energy and
humidity profiles over Lake Erie and the surrounding area will
be instituted. The study would be made primarily to develop an
estimate of the total evaporation from the lake water surface
for one year by monthly increments, and to develop techniques
for estimating evaporation routinely from any or all of the
Great Lakes from standard meteorological data.
An estimate of evaporation will be required for the water
budget study to determine the net movement of water into and
out of the lake. Wind stress data collected in the study will
also be needed in the determination of relationships between
winds over the lake and water currents within the lake.
This proposal will require specially instrumented towers
for data gathering.

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Special Studies
In order to adequately evaluate water quality conditions
in the study area, to establish the significant sources of
pollution and to permit the development of a program designed
to improve or protect existing water quality the following
special studies have been included in the study plan.
Reconnaissance
A reconnaissance of the river system and lake ranges
should be carried out before the project proper is instituted.
This preliminary survey would determine the feasibility of
the ranges to be sampled and would work out some of the factors
which would unduly delay a larger scale study. Travel times
and various routes of travel could be verified and it would
provide valuable data for planning the larger survey. The
extent and nature of the work could be laid out and sampling
on a scale that could be done by one or two laboratories would
be undertaken. Bacterial dilutions and laboratory schedules
would be test run. Surveys would be made to determine the
water quality profile in regard to time, both under dry weather
and storm conditions.
Carbon Filter Studies
In a study of this type it is necessary to determine
trace quantities of organic pollutants that are present in
such quantities as to be undetected by methods other than the
carbon filter technique. Carbon filter stations should be
located at tributary sites and at such places along the
watercourse as would be subject to changing conditions or
organic loading. Wo valid and firm stationing of the carbon
filter stations can be made without an on-site survey; however,
the following locations should be considered in selecting sites
for carbon filter units:
Raisin River
Maumee River
Black River
Cuyahoga River
Buffalo River
Selected water intakes
Lake Erie

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Storm Water Overflows
The pollutional load from combined sanitary and storm
water sewers in Cleveland, Toledo and other Lake Erie cities
is a major problem. This problem defies any easy economical
solution- The effect and magnitude of this type of pollutional
loading is difficult to assess because of the great variety of
storms and runoff conditions. An extensive investigation of
combined sewer overflows should be included, in the study. Such
an investigation would include both analysis of combined sewer
overflows and the impact of such overflows on the receiving
body of water.
This study would involve instrumentation of selected
areas of the cities with gaging facilities to measure the
diverted sewage and the raw sewage before diversion. The
quantity and quality of the discharge to the lakes and/or river
can thus be evaluated for a specific storm. Extrapolation from
one type of storm and runoff area to include a whole metropolitan
area is risky. Similar storms should produce a like pattern;
however, storms of other intensities and durations would be apt
to produce a pollutional profile which could be vastly different.
Sufficient storms should be observed to permit developing a nest
of curves which would be of considerable value in predicting
spillage from various storm patterns which would be used in
evaluating the impact of future combined sewer losses on receiving
waters.
Bacteriological Studies
In addition to using the indicator organisms to determine
bacteriological water quality, a study will be undertaken to
determine the presence of pathogenic forms.
The quantity of pathogenic forms passed from the consoli-
dated population of a large metropolis through domestic wastes
into the sewers, streams and lakes adjacent to the region should
without doubt be considered in a Public Health survey attempting
to assay the quality of public waters subject to consumption and
personal contact, either accidental, or recreational. In con-
sidering the elimination or reduction of pathogenic forms (entero-
viruses, amoebic cysts, eggs of parasitic worms, such as round-
worms, tapeworms, hookworms, Ascaris, and Shistosoma, known to
survive normal disinfection practices,) certain authorities
have suggested leaving the burden of disinfection on the water
treatment plant. Such a suggestion ignores all other public
health ramifications where highly concentrated populations have

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contact with polluted water at times, virus-laden, or loaded
with tuberculosis organisms (from sanatoria), or parasitic
forms. It is, therefore recommended that special attention
in the form of pathogenic and parasitological testing "be
undertaken at sites known to "be adjacent to outfalls of
either raw or treated sewage. Techniques in the detection
of viruses in sewage appear to be far enough advanced to
warrant their application in survey work.
While many pathogenic forms have been detected in
various watersand since it would not be feasible to undertake
survey testing for all such possible entities, it is felt that
work devoted to the detection of entero viruses, Salmonella and
the tuberculosis organism, and certain parasitological forms
would be both practical and of a definite contribution in gaining
information as to the relative safety of the waters in question.
The three factors of (l) the rather sudden increase in population
of large metropolitan areas plus (2) the increases in migration
of population from southern climates (U. S. southern states,
Mexico, Puerto Rico) and (3) increased world travel all contribute
to the dissemination of pathogenic entities not indigenous to
the area.
However, each of these areas of micro-biological investi-
gation are so specialized that special provisions would have to
be made to accommodate those areas of activity. Either samples
could be submitted to centers devoted to each of these lines of
investigation (viral, enteric, tuberculosis, and parasitological^
or appropriate specialists might be contracted for a short term
(such as academic personnel or summer leave).
Sludge Studies
Sludge studies other than those that are run by the
biological staff will be necessary at points and locations
other than at the selected ranges. The movement and redeposition
of sludge and silt banks, sand bars, harbor bars, underwater
deltas, submerged alluvial fans and obstructions cannot be pre-
dicted with any great precision and are subject to seasonal
growth or erosion. Unnatural deposits from industrial sources,
flocculent materials, silt, sewage solids, storm water grit,
and many other substances and materials will contribute to sludge
formation of varying types. To locate and measure the depth
of these permanent and transient sludge deposits will require
instrumentation probably operating on the sonar principle which

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will locate both the top and the bottom of the deposits. The
depth of these deposits will have to be referenced to some
permanent datum. Other supplemental data and sludge samples
can be collected by the survey crews assigned to map sludge
deposits.
The tributary surveys for sludge deposits are proposed
to be made in the Lfeumee, Sandusky, Black, Cuyahoga, Cattaraugus>
Buffalo and Niagara Rivers. At least two of the survey vessels
should be equipped with recording echo sounding instruments of
considerable precision, such as "EDO" Recording Depth Finders.
Sewage and Industrial Wastes Treatment Plants
An intensive sampling program will be carried out at
each of the major sewage treatment plants and industrial wastes
treatment plants or points of discharge of untreated industrial
wastes. This will involve round-the-clock sampling to determine
the cyclical character, strength and volume, of the waste dis-
charge from each of these sources.

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LABORATORY PROGRAM
The laboratory program will consist of a study of the water
quality in the bulk lake water, intensive study of water quality
in inshore, shallow areas adjacent to metropolitan centers, and
water quality of tributaries. In each of these study phases, em-
phasis will be directed to those water quality parameters showing
the greatest promise in differentiation of water quality. An ex-
ample of this would be the reduction in frequency of such tests
as BOD on the water mass of the lake. This test would provide
little or no valuable data where dispersion and dilution of organic
wastes are expected to be high. Similarly, more intensive analysis
of phenol concentrations would be carried out in the inshore and
tributary studies since this pollutant would be a measure of water
quality deterioration more readily traceable to waste discharges.
Biology
Biological investigations in the Lake Erie area will be
undertaken with three ultimate objectives:
(1)	To evaluate the general biological condition of the lake.
(2)	To define local or zonal effects from tributaries and
waste discharges.
(3)	To provide the biological information required for ex-
planation and interpretation of certain chemical, bac-
terial and physical data.
(4)	To guide the development of water quality control
measures that will promote desirable, and inhibit
undesirable, aquatic life.
Samples of benthic animals, attached flora, and phytoplank-
ton will be collected at stations selected to coincide with sta-
tions where chemical, physical and bacteriological measurements
will be made.
A minimum program would involve seasonal studies of the
biological parameters as outlined below:
Parameters	Frequency	Collected by _ Analyzed by
Phytoplankton Bi-weekly in streams Aquatic Samplers Chicago lab.
All samples from lake

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Parameters
Benthic fauna
Frequency
May, Aug, Oct.
Collected by
Biologists
Attached algae May, Aug, Oct.
Rooted weeds July or Aug.
Analyzed by
Field Station lab.
(assisted by Chgo.)
Field Station and/
or Chicago
Field Station-based biologists would be responsible for tri-
butary work and some of the lake cruises. Chicago-based biologists
would also work some of the lake cruises.
In general, sampling by biologists will be made indepen-
dently of chemical and bacteriological sampling. Biologists re-
quire longer times at each sampling site and only sample the site
two to four times a year. Two biologists, assisted by a driver or
boat operator, will comprise a field team.
Biological studies will be scheduled so that each biologist
spends about half his time in the laboratory in the identification
and evaluation of materials collected in the field.
Laboratory Procedures
Samples collected from the Lake waters would be screened at
the Field Station laboratories, preserved and returned to the
GLIRBP laboratory in Chicago. Here they would be processed (micro
and macro-examination) for enumeration and identification.
The laboratory analyses will consist of:
(1)	Bottom fauna identification and enumeration.
(2)	Attached plant life, including algae and fungi,
identification and measure of abundance.
(3)	Phytoplankton identification and enumeration.
Special projects will include studies on the use of arti-
ficial substrata for eutrophication evaluation; chlorophyll den-
sities of the subject waters; diurnal fluctuations of dissolved
oxygen, and electrofishing and bioassay as needed.

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Chemistry
The bulk of this program will consist of routine sampling
of the waters under study. Samples will be delivered to the field
laboratory for the analyses which are to be made there. The re-
mainder of the samples will be sent to GLIRBP laboratory in
Chicago. An outline is presented segregating the analytical tests
to be performed at the field laboratory and at GLIRBP into various
groups based on the need for repeated determinations for each pa-
rameter under study. A reconnaissance study would be used to es-
tablish the basis for selection of stations and the frequency of
sampling and analysis of the various parameters.
Field Laboratory Outline
Tests to be run:
pH
Specific Conductivity
DO
Alkalinity
Chloride
BOD
COD
Solids
(a)
(b')
Cyanide
Phenols
Turbidity
Dissolved
Suspended
Frequency of Test:
All samples collected
All samples collected
All samples collected
All samples collected
All samples collected
Offshore & Tributary samples
Offshore & Tributary samples
Offshore & Tributary samples
Offshore & Tributary samples
Offshore & Tributary samples
Special samples, waste
Each sample at selected stations
Each sample at selected stations
GLIRBP Laboratory Program Outline
Tests to be run:
I.	Selected Samples (composites)
Solids (Dissolved, Suspended)
Gross Minerals
(Na, K, Ca, Mg, Si, S04, CI,
alk. F.)
II.	Selected Stations
A. All Samples
CCE
ABS
Frequency of Test:
As comp.osited
As composited
Special samples
Each sample collected

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B. Selected Samples
Nitrogen
50% of all selected stations
(NH3, Kjeldahl, N02
NO3)
Oil
Phosphate
50$ of all selected stations
Special samples as needed
C. Selected Samples (Composites)
from Selected Stations
Toxic Metals
Samples collected
gross minerals and waste
outfalls and further
composited
(Cu, Pb, Zn, Cd, Ad, Se)
Tracr Elements -
Spectrographs
(Ag, Ba, Fe, Mn, etc)
Samples collected
gross minerals and waste
outfalls and further
composited.
Microbiological Investigations
The objectives of the microbiological investigation will be to
determine the present quality of the waters of each lake and its
drainage basin through the use of certain parameters which express
the sanitary status of the waters investigated and through the use
of other parameters which help to express other qualities of the
lake. This information will provide information as to points of
influx of domestic sewage and the wastes of certain industries, as
well as localizing areas of pollution within the lake itself.
The tests listed below will be the chief items comprising the
microbiological survey of the lake.
1.	Total coliform via membrane filter (All samples).
2.	Coliform differentiation on selected samples by elevated
temperature test (gas production from lactose, at 44.5 - 45.0°
in 24 hours).
1. Total fecal streptococci via membrane filter using K.F. Agar
plates. - Inshore and Tributary samples as indicated by
reconnaissance studies.
Parameters and Tests
Coliform Tests
Fecal Streptococcus Tests

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2. Streptococcus differentiation on selected samples via special
fermentation and metabolic tests.
Total Plate Counts
Total plate counts - All Lake samples plus selected Inshore
and Tributary samples. Procedure will be membrane filter or spe-
cial pour plates.
a.	20° C.
b.	35° C.
c.	With further special bacteriological identification
tests on selected samples.
Pathogen Detection
a.	Enteroviruses
b.	Salmonella
c.	Tuberculosis
d.	Other selected tests
Total Coliform
The coliform parameter provides information pertinent to
the quality of water and will be one of the most important of the
bacteriological tests in a survey of this nature.
Coliform Differentiation
Differentiation of the coliform encountered on selected
samples via the elevated temperature test will contribute valuable
additional information, particularly in the comparison of coliform
of fecal origin only.
Total Fecal Streptococcus
The membrane filter method of choice here reveals the pre-
sence of all the members of the enterococcus group consisting of
Streptococcus faecalis, S. faecalis variety zymogenes,
S. faecalis var. liquefaciens, and S. durans; and also other fecal
streptococci originating from humans, fowl, cows, sheep, horses
(S. salivarius, S. bovis, S„ Equinus. and biotype strains

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associated with each of these major species). However, to
differentiate which of these groups contribute to a given membrane
filter colony count, further physiological and metabolic tests are
required. These latter tests involve from 2 to 10 steps, includ-
ing tube tests and microscopical stains. The extent of these
tests for each individual colony selected would necessarily limit
their application to selected samples and the objective uses of
the data.
Total Plate Counts
When collecting data in a survey of streams and lakes, coli-
form and fecal streptococcus statistics give only partial informa-
tion on general water quality. Total plate counts with parallel
tests conducted at 20° C and 35° C in conjunction with the tests
for fecal indicators provide a more complete bacteriological es-
timate of water quality. The flora from selected total counts
will be identified so that the distribution and occurrence of bac-
terial species throughout the waters studied may be ascertained.
Pathogenic Studies
In addition to using the indicator organisms to determine
bacteriological water quality, a study will be undertaken to de-
termine the presence of pathogenic forms likely to be waterborne.
Each of these areas of pathogenic investigations are so
specialized that special provisions must be made to accommodate
those areas of activity. Either samples could be submitted to
centers devoted to each of these lines of investigation (viral,
enteric, tuberculosis, and parasitalogical) or appropriate spe-
cialists might be contracted for a short term (such as academic
personnel or summer leave).
Field Procedures
Field procedures will be governed by the necessities related
to the proper collection and subsequent processing of microbiologi-
cal samples.
Where the program permits, samples will be processed imme-
diately aboard laboratory vessels, otherwise samples will be
processed in the land-based laboratory, preferably within one
hour of collection.
Samples are to be collected at the same stations where
chemical, physical and biological tests are to be made.

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As much repetitive sampling of the selected stations as the
duration of the program permits is recommended, embracing distinct
seasonal collections.
Field procedures on each of the Great Lakes will embrace
pertinent oceanographic procedures, such as sampling at fixed
intervals from the surface, and proper geographical distribution
over all of the lake to be included in this study.
Laboratory Procedures
All basic water bacteriology procedures will be conducted
with strict adherence to (the current edition) Standard Methods for
the Examination of Water and Wastewater, or with procedures as set
forth by the Microbiology Department of Taft Sanitary Engineering
Center, Cincinnati, Ohio.
Radiochemistry
All samples for radioactivity studies will be sent back to
the Great Lakes-Illinois River Basins Project laboratory in Chicago
for analysis.
Samples are to be collected at the same stations where che-
mical, physical and biological tests are to be made. Special
samples will be collected when high results are encountered from
routine samples. Sewage treatment plant outfalls and waste out-
falls will be sampled, particularly where known radioisotope
users are involved.
Special biological samples will also be collected for
radioactivity determinations. This will include plankton on the
lake, bottom samples on the lake and tributaries and filamentous
algae on the tributaries.

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PUN FORMULATION
Engineering Analyses
A comprehensive engineering analysis will be made using
all data collected by the Lake Erie Study. These analyses
will include construction of a mathematical model of Lake Erie
and its major tributaries. Short range and long range effects
of waste loadings will be determined and water quality manage-
ment programs formulated.
Where the receiving v.raters are flowing streams, this
analysis will include: (l) identification of sources of waste
input to stream, (2) the effectiveness of collection and
treatment measures, (3) the stream flows available for assimi-
lation of residual wastes waters and the antecedent quality of
the stream flow, and (4) the needs for providing additional
dilution water, through storage regulation or diversion, to
meet the objectives of the program.
For Lake Erie itself, a different set of problems arises
in predicting the fate of pollutants and the effects on lake-
water quality. These problems include: (l) a study of the
mechanics of the mixing process of the waste waters with lake
water, (2) the effective volume of lake water in which mixing
will ultimately occur, (3)local effects such as possible short-
circuiting of partially diluted waste waters from point of
waste input to water supply takeout points, (4) the rate of
long-range buildup in concentration of persistent constituents
and the equilibrium condition, if any, toward which this con-
centration is trending, (5) the time required for this equili-
brium concentration to reach the critical level set by the
water quality objectives - or, alternatively, the maximum
growth of population and industrial activity that could be
sustained without puncturing the ceiling set by those object-
ives.
Knowledge of lake currents, permanent circulation
patterns and effective mixing volumes is essential to the
project in order to determine if the critical concentration
level might be reached in a local area adjacent to a city
water intake long before the average for the whole lake was
that high.
Economic and Demographic Studies
It is anticipated that much of the information needed

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20
for the Study Plan will be available in publications of exist-
ing agencies and study groups and the collection of necessary
data will, in many instances, be a matter of extracting in-
formation in existing reports and publications.
With regard to industrial projections, a study of
selected industries would be made; these industries would be
those which:
(1)	Exert major influence in support of growth
in economy and population of the area.
(2)	Use large quantities of water.
(3)	Have significant waste water discharges.
Most of the economic and demographic studies are expected
to be obtained through contractual services.
The Economic and Demographic Study would include the
following activities: Population studies and projections for
each sub-region or other area deaned of value to the study; an
econanic study of each of the above areas; and an analysis of
the rate and direction of population and industrial growth of
selected industries through the year 2010.
The following is an outline of the proposed Economic and
Demographic Study:
I	Population 1930-2010
A.	Standard Metropolitan Statistical Areas.
B.	County Data
C.	Community Data. Selected communities or townships.
D.	Service Area. Existing and likely future area.
E.	Density of population (persons per square mile).
II	Economy of the Area
A. Standard metropolitan statistical areas.
Employment 1950-1960
Total
Manufacturing-total
Selected Industries
Value Added by Manufacture 1939-1947-1957
Total
Selected Industries

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B. County Data
Employment 1950-1960
Total
Manufacturing-total
Selected Industries
Value Added by Manufacturer 1939-1947-1957
Total
Selected Industries
Other production measures, e.g., units, tons,
When available
Location of Key Plants
C. Major City or Sub-Area
Value Added by Manufacture
III Analysis
Analysis of rate and direction of growth, shift in
area or sub-area industry patterns and quantity of
productionj projection of population and levels of
selected industries to 2010.
Special Studies
Special studies planned for the Lake Erie watershed
include:
1.	Water Oriented Recreation
2.	Fish and Wildlife Aspects
3.	Agricultural Water Use
4.	Water Quality as Related to Land Use Practices
Negotiations will be carried out with qualified agencies
on a reimbursible basis for conducting such studies as items
1 and 2.

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22
FIELD STATION ORGANIZATION
The Lake Erie Field Station, located in Cleveland, Ohio,
will operate as a branch of the Great Lakes-Illinois River
Basins Project. The Field Station Director will be responsible
to the GLIRB Project Director, and will be in direct charge of
all activities of the Station. (See Organization Chart,
Figure 5.)
Certain activities will be conducted from GLIRB Project
headquarters in Chicago. These include economic-demographic"
studies, land use managsnent practices in relation to water
quality, and lake current measurements. Contract arrangements
will be made through Chicago headquarters for cooperative
studies by the Bureau of Outdoor Recreation, and by the Bureau
of Sport Fisheries and Wildlife, U. S. Department of the
Interior.
Activities in the Detroit Metropolitan Area and environs
will be the responsibility of, and directed by, the Director of
the Detroit-Lake Erie Project, located at Gross lie, Michigan.
The Lake Erie Field Station Director (Cleveland office) is
responsible for coordinating efforts with the Detroit Project
to prevent overlap or omissions in the study.

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LAKE ERIE STUDY ORGANIZATION CHART
GLIKBP PROJECT
DIRECTOR
CONSULTANTS
| LAKE ERIE FIELD STATION
-]	DIRECTOR
LABORATORIES
Bacteriology
Biology
Chemistry
Special Studies
(By GLIRBP)
FIELD OPERATIONS
Hydrology
Sampling
Special Studies
J	
PLANNING & REPORTS
Basic Data
Economic & Demographic
(Ey GLIRBP)
Engineering
ADMINISTRATIVE
OFFICER

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TIME SCHEDULE
Figure No. 6 is a bar chart indicating the proposed
starting times and duration of the proposed activities in-
cluding: (l) procurements of personnel and equipment, (2)
the setting up of the Cleveland laboratory, and (3) the con-
ductance of field sampling and various special studies.
Preliminary work is to begin in the first half of FT
63 with full scale operations commencing in the first quarter
of FY 64 and continuing for one year. The total study period
should last approximately two years.

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Great Lakes-Illinois River Basins Project
Lake Erie Work Schedule
Inventory
Fiscal Year 1
1962
1963
1964
1965
1966
7/1H

8/31

1
Reconnaissance and
Sampling

4/1
6/30


Industrial Waste
Sampling

9
fit ^"1 V
m 8/31
1
Stream Sampling

7/1

6/30

Lake Sampling

7/1

6/30

Lake Currents





^/± 12/1 I
Meteorology

2/1


Hydrology



2/1 11/30
Special Studies

4/1
8/31

Storm Water Spillage
Sludge Deposits


1
1


Beach Studies
Fishery


1
1


Economic and Demographic
Studies

l/l

6/30

Final Report


12 A fefiftfltttEBK

6/30

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