SAMPLING AND ANALYSIS PROJECT PLAN
FOR
A BIOTOXICITY AND CHEMICAL CONTAMINANTS CHARACTERIZATION
OF
THE LOWER CALCASIEU RIVER ESTUARY
LOUISIANA
LOUISIANA DEPARTMENT OF ENVIRONMENTAL QUALITY
WATER POLLUTION CONTROL DIVISION
BATON ROUGE, LOUISIANA
U.S. ENVIRONMENTAL PROTECTION AGENCY
WATER MANAGEMENT DIVISION
REGION VI
DALLAS, TEXAS
JUNE 1988
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SAMPLING AND ANALYSIS PROJECT PLAN
FOR
A BIOTOXICITY AND CHEMICAL CONTAMINANTS CHARACTERIZATION
OF
THE LOWER CALCASIEU RIVER ESTUARY
LOUISIANA
LOUISIANA DEPARTMENT OF ENVIRONMENTAL QUALITY
HATER POLLUTION CONTROL DIVISION
BATON ROUGE, LOUISIANA
U.S. ENVIRONMENTAL PROTECTION AGENCY
WATER MANAGEMENT DIVISION
REGION VI
DALLAS, TEXAS
JUNE 1988
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TABLE OF CONTENTS
SECTION PAGE
I. Project Description 1
A. Introduction 1
6. Project Objectives 3
C. Project Scope 3
1. Study Area 3
2. Participants 4
3. Monitoring Approach and Parameters 4
II. Data Objectives and Usage 9
A. Effluent and Ambient Water Toxicity 9
B. Effluent and Ambient Water Chemistry 10
C. Sediment Toxicity and Chemistry 10
III. Field Sampling Procedures 11
A. Field Water Quality Measurements 11
B. Ambient Water Sample Collection 11
C. Effluent Sample Collection 11
D. Sediment Sample Collection 12
IV. Laboratory Analytical Procedures 15
V. Sample Custody Procedures 15
VI. Quality Assurance Procedures 15
A. Calibration Procedures and Preventative Maintenance 15
B. Performance and System Audits 20
C. Corrective Actions 20
D. Data Quality Review 20
E. Data Quality Requirements 20
1. Representativeness 20
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2. Comparability 21
3. Completeness 21
4. Precision and Accuracy 21
F. Project Specific Quality Assurance Measures 21
VII. Schedule of Tasks and Products 22
VIII. Project Participant Responsibilities 22
A. Project Management 22
B. Field Coordination 22
C. Laboratory Support. 24
D. Documentation, Data Reduction, Reporting and Validation 25
E. Project Date Evaluation and Report Preparation 26
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LIST OF TABLES AND EXHIBITS
TABLE/EXHIBIT PAGE
TABLE 1 Ambient Sampling Sites for Calcasieu Estuary
Toxicity Study, 6/20-24/88 5
TABLE 2 Ambient Sampling Sites for Calcasieu Estuary
Toxicity Study, 6/27-7/1/88 6
TABLE 3 Permitted Industrial Wastewater Discharges 7
TABLE 4 Monitoring Parameters 8
EXHIBIT 1 DEQ Biological Survey Form.. 13
TABLE 5 Sample Containers and Preservative -
Ambient Waters & Effluents 14
TABLE 6 Laboratory Analytical Methods 16
EXHIBIT 2 DEQ Sample Custody Form 19
TABLE 7 Summary of Project Assignments & Schedules 23
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BIOTOXICITY AND CHEMICAL CONTAMINANTS CHARACTERIZATON
OF
THE CALCASIEU RIVER ESTUARY, LOUISIANA
I. PROJECT DESCRIPTION:
A. Introduction:
A major priority of the Louisiana Department of Environmental
Quality (DEQ) and the U.S Environmental Protection Agency (EPA) is
to evaluate and control the toxic effects of industrial and
municipal wastewater discharges on public waters. A recent national
policy statement by EPA in March 1984 (49FR9016) provides the
justification for implementing water quality-based toxics control
for point sources. A predominant emphasis of the Water Quality Act
of 1987 (P.L.100-4), which reauthorizes and amends the Clean Water
Act, is the control of toxic pollutants from both point sources and
nonpoint sources categories. Section 304(1) of the Water Quality
Act requires the EPA Regional Offices and the individual states to
categorize and prioritize waterbodies known or suspected to be
impacted by toxic pollutants whether from point sources or nonpoint
sources. Where known toxic problems exist related to discharges
from point sources, the Act requires the development and
implementation of individual source control strategies by February
1989. Where toxic problems are suspected, the Act requires the
initiation of Water Quality Assessment projects to determine whether
or not toxic impacts exist and to characterize the nature of any
toxic impacts if documented.
The State of Louisiana, through the Department of Environmental
Quality, has designated portions of the lower Calcasieu River and
some of its tributaries as priority waterbodies with known or
suspected water quality problems related to toxic pollutants
(toxics). These designations have been made pursuant to the
provisions of Section 304(1). The bases upon which the priority
waterbody designations have been made include the findings of
several recent investigations conducted by the DEQ, the Louisiana
Department of Health and Human Resources (DHHR), EPA Region VI, the
U.S. EPA Environmental Research Laboratory at Narragansett, Rhode
Island (ERL-N) and the U.S Geological Survey, Water Resources
Division, Louisiana District (USGS).
Investigations by the USGS during 1985 and 1986 and by DEQ and DHHR
in 1987 and 1988 have demonstrated the presence of chemically
contaminated sediments in localized areas and contaminated seafood
species over a broad area in the Calcasieu River estuary including
tributaries and connected coastal lakes. The findings of
contaminated sediments and seafood have resulted in the issuance by
DEQ and DHHR of seafood consumption and primary contact recreation
advisories on Bayou d'Inde and a seafood consumption advisory
covering Prien Lake and adjacent reaches of the Calcasieu River.
The sources of this contamination are suspected to be primarily
historical discharges from petrochemical and organic
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chemical manufacturing facilities in the Lake Charles/Calcasieu area
that over a number of years have resulted in the partitioning and
accumulation of selected organic chemicals in the water bottom
sediments in the vicinity of discharges. The organic chemicals
involved in the sediment contamination include polynuclear aromatics
and chlorinated benzenes, butadienes, and styrenes.
Hexachlorobenzene and hexachloro-l,3-butadiene are the significant
contaminants in the seafood. It is suspected that the contaminated
sediments are acting as a reservoir that is influencing the
bioaccumulation of selected contaminants into seafood and the
estuarine food chain. It is not known to what extent that current,
permitted discharges may be influencing bioaccumulation.
Regular periodic monitoring by DEQ since January 1987 has documented
the occurrence of elevated water column levels of low molecular
weight, halogenated volatile organics in Bayou d'Inde and the near
reaches of the Calcasieu River. These elevated levels are
undoubtedly resulting from current discharges and are not attributed
to historical discharge inputs. Compliance status reviews and
effluent testing have indicated that the area industries potentially
capable of and permitted for the discharge of organic chemicals are
essentially in compliance with their permits or applicable
administrative orders. Nevertheless, the ambient water column
levels documented are judged to be substantially elevated based upon
comparison with EPA ambient water quality criteria for the organic
compounds of concern. It is not known whether or not the volatile
organics observed are accumulating in seafood or sediments.
Collaborative efforts by DEQ, EPA Region VI and ERL-N during 1984
and 1985, indicated the sporadic occurrence of low level chronic
toxicity in the ambient waters at several locations in the Calcasieu
estuary. Toxicity tests utilized included several standard EPA test
organisms. The sea urchin (Arbacia punctulata) fertilization
inhibition test and the red alga (Champia parvula) reproduction test
exhibited the greater sensitivities in detecting low level toxicity.
The geographical extent and cause(s) of the ambient toxicity
observed earlier have not been determined and may or may not be
related to the occurrence of the presently identified organic
substances.
It is concluded that a toxic problem exists in the Calcasieu estuary
that exhibits four separate but possibly related phenomena:
1) Currently elevated ambient water levels of low molecular weight,
halogenated volatile organics (e.g. chloroform, bromoform,
1,2-dichloroethane, trichloroethylene, tetrachloroethylene,
etc.);
2) Wide ranging but low level contamination of seafood species with
higher molecular weight, chlorinated organics such as
hexachlorobenzene and hexachloro-l,3-butadiene;
3) Localized but very significant sediment contamination involving
higher molecular weight chlorinated organics (benezenes,
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butadienes, styrenes) and numerous polynuclear aromatic
hydrocarbons; and
4) Detectable, but possibly sporadic, low level chronic toxicity to
aquatic life.
The purpose of this study is to utilize the EPA marine toxicity
testing methods and through concurrent multimedia sampling and
analyses (ambient waters, effluents and sediments) determine the
geographical extent of toxic pollutants and toxicity and ascertain
any observable relationships between contaminant levels and
biotoxicity.
B. Project Objectives:
The specific objectives of this monitoring project include:
1) Evaluate the utility and sensitivity of the draft EPA chronic
toxicity test methods for aquatic life in marine and estuarine
systems (U.S. EPA 1987);
2) Determine the occurrence and geographical extent of any short
term chronic toxicity that may be exhibited in selected reaches
of the Calcasieu estuary during an anticipated low flow, warm
water temperature period;
3) Determine and document the occurrence of any effluent toxicity
that may be exhibited by selected industrial wastewater
discharges into the Calcasieu estuary;
4) Delineate the geographical extent, characterize the types,
and determine the concentration levels of chemical pollutants in
ambient receiving waters and water bottom sediments of the
Calcasieu estuary;
5) Characterize the types and determine the concentration levels of
chemical pollutants associated with industrial wastewater
discharges into the Calcasieu estuary; and
6) Ascertain any relationships between specific chemical
concentrations and measured toxicity to aquatic life.
7) The results from this study will be .evaluated in conjunction
with seafood tissue contaminants data that are being generated
by a separate, continuing monitoring project that is being
conducted by DEQ and DHHR.
C. Project Scope:
1) Study Area
The study area for this project is the lower Calcasieu River
estuary and its tributaries, Bayou Verdine and Bayou d'Inde.
Included also are three coastal lakes that are broadly connected
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to the river hydrologically: Lake Charles, Prien Lake and
Calcasieu Lake. The study area is estuarine in nature and
exhibits a regular but wide variation in salinity. The reach of
primary concern to this study is the industrialized area between
the U.S. Interstate 10 Bridge at the City of Lake Charles and
the northern end of Calcasieu Lake. In addition to water
quality impacts from industrial and municipal point sources and
urban stormwater runoff, this reach of the Calcasieu River is
hydrologically modified with extensive channel realignment and
dredging for the maintenance of a major navigation channel.
The Calcasieu River watershed constitutes Louisiana Water
Quality Management Basin 03 and is a major tributary system of
the Gulf of Mexico. That portion of this basin to be evaluated
by this project falls in Water Quality Management Basin Segments
0303, 0304 and 0309.
Thirty-five (35) ambient sampling locations have been selected
for investigation in this project. These are listed on Tables 1
and 2. Additionally, fifteen (15) industrial wastewater
effluents from ten area industrial facilities have been selected
for analysis based upon evaluation of all area dischargers for
potential to contribute to the problems observed. The
industrial discharges are listed in Table 3.
2) Project Participants
Participants in this characterization project include the U.S.
EPA Region VI, Water Management Division (EPA VI), the Region VI
Environmental Services Division Regional Laboratory at Houston
(EPA Houston), the EPA Environmetal Research Laboratory at
Narragansett, Rhode Island (ERL-N), the Louisiana Department of
Environmental Quality, Water Pollution Control Division (DEQ)
and the U.S. Geological Survey, Water Resources Division,
Louisiana District (USGS).
3) Monitoring Approach and Parameters
This characterization project is designed to concurrently
collect and analyze ambient receiving waters, effluents and
ambient water bottom sediments for chronic toxicity testing
using bioassay techniques and for specific chemical contaminants
using appropriate standard physical and chemical methods. The
specific contaminants to be determined include the EPA
designated priority pollutant organic chemicals and selected
toxic metals including arsenic, cadmium, chromium, copper,
mercury, lead and zinc. In addition, selected conventional
parameters will be monitored for water quality assessment and
evaluation. These are listed in Table 4.
The monitoring project field activities will be conducted on a
two week schedule. The first week will focus on Bayou d'Inde
and the associated discharges to Bayou d'Inde, Prien Lake and
the adjacent reach of the Calcasieu River. The second week will
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TABLE 1
AMBIENT SAMPLING SITES
FOR
CALCASIEU ESTUARY TOXICITY STUDY
JUNE 20, 1988 - JUNE 24, 1988
PROJECT # LOCATION DESCRIPTION
CAL 1 PPG Canal Immediately
downstream of Mobil Bridge #2
(PPG 001)
CAL 2 PPG Canal immediately downstream
of Mobil Bridge #3, 1/4 mile
upstream from Bayou d'Inde
CAL 3 PPG Canal at mouth
CAL 4 Bayou d'Inde 200 yds. downstream
Little Bayou d'Inde
CAL 5 Bayou d'Inde 200 yards
downstream of CITGO 001
CAL 6 Bayou d'Inde immediately
downstream of Firestone 001
CAL 7 Bayou d'Inde 150 yards
upstream of LA Hwy. 108
CAL 8 Bayou d'Inde immediately
downstream of LA Hwy. 108
CAL 9 Bayou d'Inde 1/4 mile
upstream of PPG Canal
CAL 10 Bayou d'Inde 1/4 mile
downstream of PPG Canal
CAL 11 Bayou d'Inde 1/2 mile
downstream of PPG Canal,
1/4 mile upstream of
Calcasieu Ship Channel
(Storet #S030100010)
CAL 12 Bayou d'Inde at mouth
CAL 13 Calcasieu River Ship Channel
adjacent to mouth of Bayou d'Inde
CAL 14 Prlen Lake at mouth of cut
from Calcasieu River Ship
Channel
CAL 15 Prlen Lake 1n littoral area
along western shoreline midway
between Ship Channel "cut" and
"outlet"
CAL 16 Prlen Lake Outlet
CAL 17 Lake Charles at Rangia reef
directly east of Buoy 130 and
south of Lake Charles public
beach
CAL 18 Calcasieu River at U.S Hwy. 171
near Moss Bluff
(Storet IS030410040)
LATITUDE-LONGITUDE
To Be Determined
To Be Determined
30° 12' 33" / 93° 18' 01"
30° 11' 69" / 93° 20' 55"
30° 12' 09" / 93° 19' 30"
To Be Determined
To Be Determined
30° 16' 32" / 93° 22' 04"
O nnl
30° 12' 35" / 93° 18' 13"
30° 16' 36" / 93° 20' 25"
30° 12' 10" / 93° 17' 39"
30° 11' 53" / 93° 17' 19"
30° 11' 50" / 93° 17' 16"
30° 11' 43" / 93° 17' 10"
To Be Determined
30° 10' 31" / 93° 17' 10"
30° 18' 26" / 93° 16' 51"
30° 17' 53" / 93° 11' 14"
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TABLE 2
AMBIENT SAMPLING SITES
FOR
CALCASIEU ESTUARY TOXICITY STUDY
JUNE 27, 1988 - JULY 1, 1988
CAL 21
CAL 22
CAL 23
CAL 24
PROJECT # LOCATION DESCRIPTION
CAL 18 Calcasieu River at U.S. Hwy. 171
near Moss Bluff
(Storet #S030410040)
CAL 19 Bayou Verdine Immediately upstream
of Vaughn Road Bridge (Westlake)
CAL 20 Bayou Verdine approximately 1/2 mile
downstream of Truesdale Road
(downstream of Vista 001 and upstream
of Conoco 001)
Bayou Verdine at Interstate 10
Bayoih Verdine at road approximately
(fl/2jnile upstream from Coon Island
ti>op barge slip
Bayou Verdine at mouth (barge slip)
Calcasieu River Coon Island Loop
midstream adjacent to PPG South
Terminal Dock (west side of loop)
CAL 25 Calcasieu River Coon Island Loop
midstream and approximately 200 yds.
SSE of 01 In 010 (east side of loop)
CAL 26 Calcasieu River Clooney Island Loop
midstream adjacent to "Mike Hooks"
dock (east side of loop)
CAL 27 Calcasieu River Clooney Island Loop
midstream adjacent to southwest
corner of Clooney Island (west side
of loop)
CAL 28 Calcasieu River at Buoy 112 between
between Port of Lake Charles and
Prien Lake
CAL 29 Calcasieu River Ship Channel at Buoy
108, approximately 1.3 miles south-
west and downstream of Bayou d'Inde
CAL 30 Calcasieu River at Buoy 106,
approximately 1/4 mile upstream of
Vincent Landing
CAL 31 Calcasieu River immediately
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TABLE 3 - PERMITTED INDUSTRIAL WASTEWATER DISCHARGES
SOURCE NPDES OUTFALL COLLECTED PROJECT LABORATORY PROJECT LABORATORY
IDENTIFICATIONS NUMBER NO. BY BIOTOXICITY TESTING SPECIFIC CHEMICAL
ANALYSIS
PPG Industries, Inc.
LA0000761 "
001
DEQ
ERL-N
EPA-Houston
Westlake Polymers, Inc.
LA0071382H
LA0071382"
001
DEQ
DEQ
EPA-Houston
Westlake Polymers, Inc.
007
DEQ
DEQ
EPA-Houston
Firestone Synthetic
LA0003824 -
001
DEQ
DEQ
EPA-Houston
Rubber & Latex Co.
C1tgo Petroleum Corp.
LA0005941
001
DEQ
DEQ
EPA-Houston
Occldential Chemicals
LA0069850
002E
DEQ
DEQ
EPA-Houston
Vista Chemicals
LA0003336
001
DEQ
ERL-N
EPA-Houston
Conoco, Inc.
LA0003026'
001
DEQ
DEQ
EPA-Houston
PPG Industries, Inc.
LA0000761"
004
DEQ
DEQ
EPA-Houston
011n Corporation
LA0005347^
010
DEQ
DEQ
EPA-Houston
01 in Corporation
LA0005347
028
DEQ
DEQ
EPA-Houston
01 in Corporation
LA0005347
001
DEQ
DEQ
EPA-Houston
Citgo Petroleum Corp.
LA0005941
003
DEQ
DEQ
EPA-Houston
Himont U.S.A., Inc.
LA0003689'
001
DEQ
DEQ
EPA-Houston
W.R. Grace
LA0001333 <-
001
DEQ
DEQ
EPA-Houston
RECEIVING
WATERBODY
PPG Canal/Bayou d'Inde
Bayou d'Inde
Bayou d'Inde
Bayou d'Inde
Bayou d'Inde
Bayou d'Inde
Bayou Verdine
Bayou Verdine
Bayou Verdine
Calcasieu River Coon Island Loop
Calcasieu River Clooney Island Loop
Calcasieu River Clooney Island Loop
Calcasieu River
Calcasieu River
Calcasieu River
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TABLE 4 - MONITORING PARAMETERS
FIELD MEASUREMENTS (measured In Situ in the field)
Air Temperature ( °C)
Water Temperature ( C)
Dissolved Oxygen (mg/L) 2
Specific Conductance (micromhos/cm )
Salinity (parts per thousand-grams /L)
pH (Standards Units)
Secch Disk Visibility Depth (inches)
Total Residual Chlorine (mg/L) (sampled & measured in field)
CONVENTIONAL WATER QUALITY PARAMETERS (effluents & ambient water)
Alkalinity (mg/L)
Hardness (mg/L)
Chloride (mg/L)
Ammonia Nitrogen (mg/L)
Total Suspended Solids (mg/L)
Total Dissolved Solids (mg/L)
Total Organic Carbon (mg/L) - effluent & ambient water
(ug/g) - sediments
Turbidity (Nephelometric Turbidity Units)
Sulfide (mg/1)
METALS (Total and Dissolved in effluents & ambient water)
(Total recoverable in bottom sediments)
Arsenic
Cadmi um
Chromium
Copper
Mercury
Lead
Zinc
Manganese
Iron
ORGANICS - (Refer to EPA analytical methods 624 and 625 for analytes to be
determined by GC/MS/DS)
BIOTOXICITY
Arbacia punctulata -
Champia parvula -
Cyprindon variegatus
Menidia beryl!ina -
Mysidopsis bahia -
Ampelisca abdita -
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Sea Urchian fertilization test (ERL-N, ambient and
effluents)
Marine Red algal reproductive test (ERL-N, ambient
and effluents)
- Sheephead minnow larval survival and growth test
(DEQ, effluents only)
Inland silverside larval survival and growth test
(ERL-N, ambient and effluent)
Mysid survival, growth and fecundity test (ERL-N,
ambient and effluents)
estuarine amphipod sediment toxicity test (ERL-N,
sediments only)
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focus on Bayou Verdine, the Calcasieu River and Ship Channel,
applicable effluents, Lake Charles and Calcasieu Lake (see
Tables 1 and 2). All effluents, ambient waters and sediments
for specific chemical analyses will be collected one time per
location and shipped to the respective laboratory assigned. The
primary effluents (PPG 001 for week one and VISTA 001 for week
two) and all ambient waters for toxicity testing will be
collected three times during each study week (MWF) and shipped
overnight to ERL-N. The remaining effluents will be sampled
three times during each study week and transported to the DEQ
Biotoxicity Laboratory. Sediments for toxicity testing will be
sampled one time per location and will be shipped to ERL-N.
All sediment samples will consist of composites of at least
three separate grabs. All ambient water samples will be
collected as depth-integrated composites. Effluents will be
collected as grab samples for both toxicity testing and specific
chemical analyses.
Three reference sampling locations have been selected for
monitoring that are located out of the direct influence of
industrial discharges. A station upstream of the study area and
above the U.S. Army Corps of Engineers saltwater barrier has
been located in the Calcasieu River at U.S. Hwy. 171 (CAL 18) at
Moss Bluff. This is a freshwater location upstream of all study
area industrial discharges. A second reference (CAL 17) is
located in Lake Charles just east of the Calcasieu River Ship
Channel and adjacent to the City of Lake Charles public beach.
This location is also presumably upstream of the influence from
area industries. The third reference (CAL 35) is located
mid-lake in Calcasieu Lake, an important seafood resource area
and presumably downstream of the industrial discharge impact
area.
II. DATA OBJECTIVES AND USAGE:
All data generated by this project will be used for evaluating receiving
water impacts and are intended to provide information for the assessment
and development of permit limits, either as part of the EPA Region VI
and DEQ third round permit process or for the development of individual
control strategies and the re-opening/revision of current permits to
fulfill the requirements of Section 304(1) of the Water Quality Act of
1987. The results will also provide informatin for the assessment and
development of state water quality standards.
A. Effluent and Ambient Hater Toxicity:
Data provided by this study of effluent and receiving water
toxicities can be used to evaluate the relative sensitivities of the
various testing methods and organisms utilized. The appropriateness
of each respective test for regulatory application in coastal
Louisiana and Region VI can be evaluated. Project toxicity results
can be used to determine which types of toxicity tests should be
used in third round permits for area industries. Based upon the
degree of toxicity found in each respective effluent, the need can
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be determined for (1) further tsting and evaluation before
finalizing revised or third round monitoring requirements, (2) whole
effluent toxicity limits, and/or (3) toxicity reduction evaluations.
B. Effluent and Ambient Water Chemistry:
Specific chemical analytical results for effluent and ambient waters
will be used in conjunction with receiving water low flow dilution
calculations and compared with EPA priority pollutant water quality
criteria and State of Louisiana water quality standards. A
determination can then be made of the need for application of BAT
effluent guidelines or for development of water quality-based permit
limits. No third round permits have yet been issued to Calcasieu
area industries and all second round permits in place include BPJ
limits for toxic pollutants and either BPJ, BAT or water
quality-based limits for conventional pollutants. Since a number of
priority pollutant volatile organic compounds (VOCs) have been
documented by DEQ to be regularly exceeding the EPA recommended
water quality criteria for protection of human health, more
stringent permit limits for certain organics are indicated.
Concurrent with this project, DEQ is utilizing RECEIVE II, a
hydrodynamic and water quality model used in 1984-86 by the State
and EPA Region VI to determine total maximum daily loads (TMDLs) for
conventional pollutants in the Calcasieu estuary, to provide input
to T0XI4. This latter model is a toxics transformation, transport
and fate model that will be used to determine TMDLs for
approximately ten organic pollutants that DEQ has determined to
regularly exceeding the respective EPA water qulity criteria in the
ambient water column in Bayou d'Inde and adjacent reaches of the
Calcasieu River. Data generated by this project, along with
previously collected data, will be used to support the modeling
effort. The modeling effort will be used to determine whether
application of BAT guidelines or water quality-based permit limits
will be necessary to control individually each pollutant of concern
so that the existing and proposed State of Louisiana Water Quality
Standards are attained in the ambient environment.
C. Sediment Toxicity and Chemistry:
Sediment toxicity and specific chemical analytical data will be used
to (1) assess the geographical extent and severity of sediment
contamination, (2) assess the role of sediment contamination in
contributing to bioaccumulation, and (3) evaluate the degree of
impairment of other designated beneficial uses assigned to the
waterbodies of the study area by the Louisiana Water Quality
Standards. Where substantial contamination is documented and is
assessed to be contributing to bioaccumulation or causing other
designated use impairment, project data will provide a justification
for and be used in the decision-making process concerning
environmental remediation.
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III. FIELD SAMPLING PROCEDURES:
A. Field Hater Quality Measurements;
Field parameters will be measured in situ at each ambient monitoring
station using Hydrolab Surveyor II or Series 4000 multiprobe
analyzers. Field parameters to be measured and recorded include
those listed on Table 4. The points of reading for field data
(except residual chlorine and Secchi disk) will be midstream at the
depths indicated below:
Station Depth Reading Depths
< 1.0m 1/2 total depth
> 1.0m £ 4.0m 1.0m below surface & 0.3m above bottom
> 4.0m 1.0m below surface & at 3m intervals to
0.3m above bottom
Total residual chlorine will be collected at 1.0m depth using a
sewage sampler fitted with a clean BOD bottle. Total residual
chlorine samples are to be analyzed in the field as soon as possible
after collection at the USGS Mobile Laboratory which will be located
as a base of field operations at the Prien Lake boat ramp.
Field data and observations will be recorded on the DEQ "Biological
Survey Form" (Exhibit 1). All data and information concerning
observations should be completed in the appropriate sections. Those
sections that are not applicable to this project should be marked
"N/A".
B. Ambient Water Sample Collection for Laboratory Analyses:
The point of collection for ambient water samples will be midstream
at each station. Samples will be collected as depth-integrated
composites using a submersible Johnson-Keck Trace Organics Pump
Sampler provided by USGS. Samples for V0C analyses will be
collected using a stainless-steel sewage sampler. All water samples
will be transferred to the appropriate sample containers and
preserved according to the schedule in Table 5. During each of the
two primary study weeks, the respective ambient water quality
stations will be sampled once (Monday, June 20 or June 27, 1988 -
see Tables 1 and 2) per study week for specific chemical analyses
and three times per week for toxicity testing (MWF). Field
measurements will be taken and recorded three times per week (MWF).
C. Effluent Sample Collection:
Collection of point source effluents from the industrial outfalls
will be by grab samples. Parameter coverage will be as for ambient
water quality stations (Tables 4 and 5). Samples will be collected
at the designated outfall locations as described in the NPDES
permits for the facility outfalls listed in Table 3. The facilities
discharging to Bayou d'Inde will be sampled during the week of June
20-24, 1988 and all others will be sampled during the week of June
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27, 1988 - July 1, 1988. Effluent samples for specific chemical
analyses will be collected once per study week (M) and three times
per week (MWF) for toxicity testing. Specific conductance, pH,
temperature and total residual chlorine will be determined and
recorded for each sampling event. Instantaneous flow data for the
time of sampling will be obtained from the industrial permittee for
each effluent sampling event. Totalized 24 hour flow data for each
day (including Sundays prior to surveys - SMTWTF) will be obtained
from each permittee. Field collectors will note and record any
relevant observations concerning effluent conditions. Information
regarding any abnormal operations, process upsets, spills, etc. will
be obtained from the permittees.
D. Sediment Sample Collection:
Water bottom sediments will be collected at all ambient water
quality sampling locations as listed in Tables 1 and 2. Samples
will be composites. At Bayou d'Inde and Bayou Verdine locations,
composites will be made by mixing a minimum of three grabs collected
along a transect of the station. For Calcasieu River/Ship Channel
locations, two grabs equally spaced across each littoral shelf plus
two grabs from the dredged channel will be composited (total of
six). For lake stations, a minimum of three randomly spaced grabs
taken from an approximate 5 meter square area will be composited.
Grabs will be collected using a stainless steel Petite Ponar bottom
sampler. Grabs will be composited and thoroughly mixed in stainless
steel trays or buckets. Composited sediments will then be
transferred to cleaned and pre-rinsed wide mouth jars. Sediment
jars (240 ml) for metals analyses will be pre-rinsed with reagent
grade nitric acid and deionized water in the lab, and finally rinsed
in the field with ambient water collected at 1 meter depth with a
sewage sampler from the respective sampling station. Sediment jars
(480 ml) for extractable organics will be pre-rinsed with nanograde
hexane and deionized water in the lab, and finally rinsed in the
field with ambient water from the respective station. Sediment
samples for VOCs will be composited by removing an approximate 40-80
gram aliquot from each separate sediment grab (before mixing in
stainless steel buckets or trays) using a stainless steel spatula.
Care will be taken in gently removing each sediment aliquot and
placing them in 240ml glass wide mouth jars. Containers will be
filled to the lip and headspace must be eliminated to the maximum
extent possible, using ambient water as necessary.
All equipment (Petite Ponars, trays, buckets, sewage samplers) used
for sampling sediments will be decontaminated and rinsed with
deionized water prior to the surveys and between stations. At each
new station, equipment will be rinsed again with ambient water
collected from 1.0 meter depth (to avoid surface film or sheen
contaminants). No solvent rinses should be used in the field except
to remove oily residues not removed by normal decontamination
procedures. In this case, nanograde hexane will be used sparingly,
allowed to evaporate, then rinsed with deionized water and ambient
water again. This should be undertaken and completed prior to any
ambient water or sediment sampling is begun at a given station.
- 12 -
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EXHIBIT 1
BIOLOGICAL SURVEY
STATION NO. SURVEY TEAM_
DATE WATUR BODY
TIME (INCLUSIVE) PARISH
BASIN/SEGMENT
STATION DEPTH SECCH1 DISC VISIBILITY
AIR TEMP. WEATHER CONDITIONS
WIND DIRECTION ESTIMATED WIND SPEED WAVE HEIGHT_
OBSERVATION OF WATER CONDITIONS
FIELD HATER QUALITY READINGS
DEPTH DEPTH DEPTH DEPTH DEPTH DEPTH_
TEMP. °C °C °C °C _°C
COND. umhos umhos umhos umhos umhos_
nwnhos mmhos mmhos mmhos mmhos
SAL. ppt ppt ppt ppt ppt
DO mg/1 mg/1 mg/1 mg/1 mg/1
pH S.U. S.U. S.U. S.U. S.U.
BATTERY BATTERY BATTFRY BATTERY BATTERY BATTERY_
INSTRUMENTATION
PROPERTY TAG NUMBER 320-02- 320-02- 320-02-
HABITAT DESCRIPTION
FRESHWATER RIVER BAY POOL
ESTUAR1NE LAKE SWAMP RIFFLE_
MARINE STREAM UPLAND TIDAL
1ND/MUN MARSH LOWLAND FREEFL0WING_
VEGETATION(TERRESTRrAL)
(AQUATIC)
SOIL/SEDIMENT TYPE CLAY % SILT
(ESTIMATION) SAND % DETRITAL
FISH
POISON '
SEINES
ELECTROFISHING_
TRAWL
NETS/TRAPS_
OTHER
BENTHOS AND OTHER HACROINVFRTEBRATES (SPECIFY GEAR S SAMPLE TYPE)
# OF REPLICATES COMPOSITED: YES NO
BTOMONITORING
VOLUME OF SAMPLE_ (COMPOSITE OR GRAB) TIME (PRESERVE IMMEDIATELY ON ICE)
LABORATORY SAMPLES
SAMPLE# SAMPLE# SAMPLE#
DEPTH DEPTH DEPTH
LAB LAB LAB
- 13 -
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TABLE 5 - SAMPLE CONTAINERS ft PRESERVATIVES - AMBIENT WATERS ft EFFLUENTS
PARAMETER
CONTAINER
VOLUME NUMBER
TYPE
PRESERVATIVE
Extractable organics 1.0 gallon
(Acid & Base/Neutral)
Volatile organics (Vocs) 40ml septum
vial
Total Phenolics (4 AAP) 1 liter
Total Metals (As, Cd, Cr 100 ml
Cu, Hg, Pb, Zn, Mn, Fe)
Dissolved Metals (As, Cd, 100 ml
Cr, Cu, Hg, Pb, Zn, Mn, Fe)
Total Residual Chlorine 400 ml
Ammonia Nitrogen 1 liter
Sulfide 1 liter
Alkalinity, Hardness, 1 liter
Chloride, TSS, TDS,
Turbidity
TOC 100 ml
Biotoxiclty 2.5 gallon
Glass w/teflon lined cap Cool on wet ice in field
Glass w/teflon septum cap Cool on wet ice in field
Glass w/teflon lined cap
Plastic nalgene bottle
Plastic nalgene bottle
Glass BOD bottle
Plastic cube container
Plastic cube container
Plastic cube container
Plastic nalgene bottle
Plastic cube container
HgSO^ to pH <2, cool on wet ice in field
HNOj to pH <2, cool on wet ice in field
* Filter In field, HNO, to pH <2, cool on wet
1ce 1n field
Cool on wet 1ce 1n field
HjjSO^ to pH <2, cool on wet Ice in field
NaOH to pH >9, 2m1 zinc acetate (2N), cool on
wet ice in field
Cool on wet 1ce in field
HC1 to <2, cool on wet ice 1n field
Cool on wet 1ce in field
* Metal samples can be collected originally in 1 liter cube containers, transported to field mobile lab, a portion filtered for
dissolved metals analyses, and then transferred to separate 100ml nalgene bottles for shipment to laboratory.
-------
IV. LABORATORY ANALYTICAL PROCEDURES:
All laboratory analytical procedures will follow U.S. EPA approved
methods as published in Methods for Chemical Analysis of Mater and
Waste, EPA Publication Number EPA-600/4-79-020, revised 1983; Methods
for Urganic Chemical Analysis of Municipal and Industrial Wastewater,
Publication Number EPA-600/4-82-057; Test Methods for Evaluating Solid
Waste, EPA Publication Number SW846, Third Edition, Volumes 1A (metals)
and IB (organics); and Standard Methods for the Examination of Water and
Wastewater, 16th Edition, American Public Health Associaton. Methods
for toxicity testing will follow Short Term Methods for Estimating the
Chronic Toxicity of Effluent and Receiving Waters to Marine an?
Estuarine Organisms, Publication Number EPA-600/4-87-028 (final draft
October 1987).
Listed in Table 6 are the parameter specific methods that will be
followed by the laboratories participating in this project.
V. SAMPLE CUSTODY PROCEDURES:
Appropriate documentation of sample custody will be undertaken and
maintained for all samples collected during this project in accordance
with established EPA and DEQ policies and procedures. The DEQ Sample
Chain of Custody Form (Exhibit 2) will be initiated by the field
collectors. Transfer of custody to and within the respective project
laboratories will be documented in writing on the Chain of Custody Form.
When samples are shipped by a common carrier, a bill of lading will be
obtained, and retained as part of the custody record.
VI. QUALITY ASSURANCE PROCEDURES:
The sampling and analytical activites of this project will follow the
established quality control/quality assurance procedures of the EPA, DEQ
and US6S. In general, the sampling procedures and sample documentation
will follow the requirements of the DEQ/WPCD Quality Assurance Plan for
Water Monitoring and Analysis (draft submitted to Region VI in 9/87).
Specific sampling procedures are detailed in Section III of this
sampling and analysis plan. Laboratory activities will be conducted in
accordance with the respective quality assurance plans for the
participating laboratories and the EPA approved analytical methods will
be followed for the designated project parameters. All equipment,
glassware, and sample containers will be prepared, cleaned and utilized
in accordance with good laboratory practice and the requirements of the
applicable parameters and analytical methods.
A. Calibration Procedures and Preventative Maintenance:
All field instruments will be calibrated daily in accordance with
the applicable EPA approved standard method and the manufacturers'
specifications. Preventative maintenance and cleaning will be in
accordance with the manufacturers' specifications. Laboratory
instrumentation will be calibrated and tuned on a daily basis or as
required by the applicable EPA approved standard method and the
- 15 -
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TABLE 6
PARAMETER
MATER « EFFLUENTS:
Extractable Organlcs (Acid & Base/Neutral)
Volatile Organlcs (VOCs)
Total PhenolIcs
Metals (Total & Dissolved)
Arsenic
Cadmlurn
Chromium
Copper
Iron
Lead
Mercury
Z1nc
Manganese
LABORATORY ANALYTICAL METHODS
METHOD CITATION
EPA Method 625 - Methods for Organic Chemical Analysis of Municipal and
Industrial Wastewater, EPA - 600/4-82-057
EPA Method 624 - Methods for Organic Chemical Analysis of Municipal and
Industrial Wastewater, EPA - 600/4-82-057
EPA Method 420.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 206.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 213.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 218.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1$83) EPA-S0O/4-7$-02O
EPA Method 220.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 236.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 239.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 245.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 289.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 243.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
Instrumental analytical methodologies for total and
fraction is based upon filtration in the field with
dissolved metals are the same; analytical differentiation of the dissolved
a .45 micron filter prior to sample preservation with nitric acid.
-------
PARAMETER
Ammonia Nitrogen
Total Alkalinity
Hardness
Chloride
Turbidity
Total Suspended Sol Ids
Total Dissolved Solids
Total Organic Carbon
Total Residual Chlorine
Sulfides
TABLE 6 - CONTINUED
METHOD CITATION
EPA Method 350.3 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 310.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-S00/4-74-020
EPA Method 130.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 325.3 - Methods for Chemical Analysis of Water and Wastes
(Revised March' 1983) EPA-600/4-79-020
EPA Method 180.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 160.2 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-600/4-79-020
EPA Method 160.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EpA-6(W4-79-020
EPA Method 415.1 - Methods for Chemical Analysis of Water and Wastes
(Revised March 1983) EPA-B00/4-79-020
To be decided upon (EPA or APHA Standard Methods)
Method 427C - Standard Methods for the Examination of Water and
Wastewater, 16th Edition, APHA
-------
PARAMETER
Biotoxlcity
Sea Urchin
Red alga
Sheepshead Minnow
Inland Sllverslde
Mysid
. SEDIMENTS:
Extractable OrganIcs (Acid & Base/Neutral)
Volatile Organlcs (VOCs)
Total Recoverable metals
Toxicity
Estuarlne amphlpod Ampelisca
TABLE 6 - CONTINUED
METHOD CITATION
EPA Method 1008 - Short Term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine
and Estuarine Organisms, EPA-600/4-87-028
EPA Method 1009 - Short Term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine
and Estuarine Organisms, EPA-600/4-87-028
EPA Method 1004 - Short Term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine
and Estuarine Organisms, EPA - 600/4-87-028
EPA Method 1006 - Short Term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine
and Estuarine Organisms, EPA - 600/4-87-028
EPA Method 1007 - Short Term Methods for Estimating the Chronic
Toxicity of Effluents and Receiving Waters to Marine
and Estuarine Organisms, EPA - 600/4-87-028
EPA Methods 8250 or 8270 with appropriate extraction and cleanup
methods - Test Methods for Evaluating Solid Waste, U.S. EPA publication
SW846, 3rd Edition, November 1986
EPA Methods 5030 and 8240 - Test Methods for Evaluating Solid Waste,
U.S. EPA publication SW846, 3rd Edition, November 1986
EPA Method 7000 series for Atomic Absorption Spectroscopy with Acid
Digestion Method 3050 - Test Methods for Evaluating Solid Waste, U.S.
EPA publication SW846, 3rd Edition, November 1986
EPA Method in development at ERL-N
-------
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MAlLK rULLUIlUW UUHIHUL UXVIOIVK wruirt.1. n
KAME:
r
No. of Containers c
c
Duplicates Retained
/////////// j
ADDRESS:
i /
LOCATION:
PERMIT #: TEAM LEADER:
SAMPLE IIUMBER
V
e
G
CJ
<0
u
o
TIME
DATE
OUTFALL
/ PRESERVATIVES
/REMARKS/RANGES
DUPLICATES ACCEPTED BY:
NAilE: SIGNATURE:
Aliq
Grabs
Team
Witness Nane/Slgnature
Title
CHAIIJ OF CUSTODY
RELINQUISHED BY:
DATE
TIME
RECEIVED BY:
RECOMMENDED SAI1PLE DISPOSITION:
FMAL SAMPLE DISPOSITION/DATE:
rn
x
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HH
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ro
-------
manufacturers' specifications. Preventative maintenance will be
according to good laboratory practice and the manufacturers'
specifications.
B. Performance and System Audits:
Performance audits are conducted routinely at the respective
laboratories participating in this project. Questions regarding the
frequency, nature and results of these audits can be directed to the
individuals listed in Section VIII, Part C of this sampling and
analysis plan. Field and laboratory audits to evaluate biotoxicity
ad specific chemical analytical procedures may be performed by EPA
Quality Assurance Officers during the course of this project.
C. Corrective Actions:
Individuals responsible for taking corrective actions to ensure that
project activities follow those specified by this sampling and
analysis plan and the referenced EPA approved methods are as follow:
Field operations - Field Project Officer (see Section VIII, Part B)
Laboratory operations - Designated individuals responsible for the
respective agency laboratories as detailed
in Section VIII, Part C.
D. Data Quality Review:
Data quality review will include the following elements:
* Identify cases where control mortality in bioassays exceeds 10%;
* Determine if sampling, preservation, custody and holding procedures
deviated from standard procedures;
* Determine if laboratory analytical methods deviated from specified
EPA approved methods as detailed in this sampling and analysis plan;
* Evaluate laboratory analytical data for percent recoveries, field
blanks, laboratory blanks, intralaboratory duplicates, spiked
samples, field replicates and interlaboratory duplicates;
* Assess any other information or observations that may suggest data
inadequacies;
* Compile a summary of the above elements for inclusion in the final
project report.
E. Data Quality Requirements:
1) Representativeness
Effluent samples will be collected as grabs. Composite sampling
could possibly improve representativeness but is not feasible
- 20 -
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within the scope of the project because of logistical and
resource limitations. Depth-integrated ambient water samples
will be collected and should be representative of the water
column at a given ambient sampling location. Sediments will be
collected as spatial composites (see Section III, Part D) to
increase representativeness.
2) Comparability
The same primary parameters will be analyzed at all locations
including reference stations, and should be directly comparable
between stations and media, particularly within the same study
week.
3) Completeness
There should be no reductions in the number of stations,
parameters and analytical tests to be conducted. Care must be
taken in the packing and shipment of samples to prevent
breakage. Laboratories should efficiently utilize sample
amounts. Timing and resources will probably not allow
resampling. Loss of any data or samples will tend to reduce the
value of the study results.
4) Precision and Accuracy
Precision and accuracy capabilities for the field water quality
measurements are provided by the manufacturers' specifications
for the Hydrolab Survey and Hydrolab 4000 Series multiprobe
analyzers and are acceptable for the purposes of this study and
in accordance with an EPA approved standard methods for the
parameters. Precision and accuracy of the selected laboratory
chemical analytical methods are described in the methods cited
in Table 6. The selected methods provide adequate precision and
accuracy for the purpose of this project. The precision of test
organisms used for toxicity testing for this project have been
determined by the ERL-N prior to this study using reference
toxicants. The precision of the standard EPA approved test
methods is in the range of +_ 30-50% and is acceptable for this
project.
F. Project Specific Quality Assurance Measures:
The following sampling and analytical Quality Assurance Measures
will be undertaken during this project:
* Field blanks consisting of laboratory deionized water will be
carried into the field during sampling. The field blanks are
intended to check for inadvertent contamination as may occur during
sampling, handling and transport. The target analytes of concern
are the VOCs and the extractable organics. Therefore, appropriate
containers with deionized water from the DEQ/WPCD laboratory will be
utilized. During the sampling for ambient waters and sediments for
week number 1, field blanks will be submitted to both the USGS and
- 21 -
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the EPA-Houston laboratories. During the second study week, field
blanks will be submitted to both the DEQ and the EPA-Houston
laboratories.
* Replicate field samples of ambient water and sediment will be
collected at selected stations and submitted to the laboratories for
evaluation of sampling variability.
* Sediment samples at selected stations will be split and submitted to
participating laboratories as duplicates for evaluating
interlaboratory and intralaboratory variability.
* Determination of which stations will be selected for replicate
and/or duplicate sampling will be made at the final survey planning
meetings on the Sunday prior to commencing each study week.
VII. SCHEDULE OF TASKS AND PRODUCTS:
The field monitoring activities for this project are scheduled to be
undertaken during two primary study weeks during which, respectively,
each of the two industrialized tributaries and adjacent reaches of the
Calcasieu River/Ship Channel will be surveyed. During the week of June
20-24, 1988, Bayou d'Inde, the associated industrial effluents, Prien
Lake and the Calcasiu River Ship Channel at the mouth of Bayou d'Inde
will be monitored. The following week, June 27 - July 1, 1988, Bayou
Verdine, the Calcasieu River, Lake Charles and Calcasieu Lake and the
associated effluents will be surveyed. It is possible that a third and
fourth week of field activities will be necessary for followup sampling
for confirmation purposes, particularly in connection with any toxicity
observed in effluents and ambient receiving waters. The details and
specific types of followup sampling cannot be predicted at this time
pending review of observations and findings from the two primary study
weeks.
It is anticipted that analytical results will be available for
compilation and evaluation by October 1988. A draft final report is
scheduled for review in early January 1989, and will be finalized,
incorporating review comments from the participating agencies by the end
of March 1989. A summary of project assignments is presented in Table 7.
VIII. PROJECT PARTICIPANT RESPONSIBILITIES:
A. Project Hangement:
Project management is provided by the Standards and Water Quality
Management Section of the Water Pollution Control Division
(LADEQ/WPCD) and the Technical Section, Water Quality Management
Branch, Water Management Division, EPA Region VI. Responsible
individuals are Michael H. Schurtz (504/342-8938) for DEQ and Philip
A. Crocker, (214/655-7145) for EPA Region VI.
B. Field Coordination:
The Surveillance Section of the DEQ/WPCD is responsible for the
- 22 -
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TABLE 7 - SUMMARY OF PROJECT ASSIGNMENTS AND SCHEDULE
Media/
Analyses
Number of Field Laboratory
Sampling Locations Responsibilities Responsibility
I. STUDY WEEK #1 - June 20-24, 1988
Effluents/toxicity 1
Effluents/toxicity 6
Effluents/chemistry 6
Ambient water/toxicity 12 *
Ambient water/chemistry 18
Sediments/toxicity 18
Sediments/chemistry 18
II. STUDY WEEK #2 - June 27 - July 1, 1988
Effluents/toxicity 1
Effluents/toxicity 9
Effluents/chemistry 9
Ambient water/toxicity 12 *
Ambient water/toxicity 18
Sediments/toxicity 18
Seiments/chemistry 18
DEQ
DEQ
DEQ
DEQ/EPA/USGS
DEQ/EPA/USGS
DEQ/EPA/USGS
DEQ/EPA/USGS
DEQ
DEQ
DEQ
DEQ/EPA
DEQ/EPA
DEQ/EPA
DEQ/EPA
ERL-N
DEQ
EPA-Houston
ERL-N
USGS
ERL-N
USGS
ERL-N
DEQ
EPA-Houston
ERL-N
DEQ
ERL-N
EPA-Houston
III. STUDY WEEKS #3*4 (To be scheduled as necessary, July 1988)
PURPOSE: Followup confirmation testing (toxicity and chemistry) on effluents
and possibly ambient waters in mixing zones, to be determined from
results of primary study weeks.
IV. LABORATORY ANALYSES AND REPORTING: July - September 1988
V. PROJECT DATA EVALUATION AND REPORT PREPARATION: October 1988 - January 1989
VI. PROJECT REPORT REVIEW/REVISION AND COMPLETION: February - March 1989
* Ambient water for toxicity testing will be collected at the following only:
Week 1 - CAL 3, CAL 4, CAL 5, CAL 6, CAL 8, CAL 9, CAL 11, CAL 12, CAL 13,
CAL 15, CAL 17, CAL 18
Week 2 - CAL 18, CAL 19, CAL 20, CAL 21, CAL 23, CAL 24, CAL 25, CAL 26, CAL 27,
CAL 30, CAL 31, CAL 34
- 23 -
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coordination and implementation of the field sampling activities.
The field project officer is Kirk A. Manuel, DEQ/WPCD Southwest
Regional Coordinator (318/491-2082). The Environmental Program
Manager for the Surveillance Section is Louis R.C. Johnson
(504/342-8930), who is responsible for allocating and assigning DEQ
field personnel and equipment to the project.
The following are operational responsibilities during the project:
* Effluent sampling for both study weeks will be conducted by
DEQ/WPCD field staff.
* Ambient water sampling for both study weeks will be conducted by
DEQ/WPCD field staff.
* Sediment sampling will be conducted by USGS, DEQ/WPCD, and EPA
Region VI staff during study week 1 and by DEQ/WPCD and EPA
Region VI staff during study week 2.
* A field operations base will be established at the Prien Lake
boat landing for sample filtration (metals), sample inventory,
and final packing for shipment or transport to the respective
project laboratories. This base will provide for logistical
support, backup equipment and supplies as well as mobile
communications. The operations base will be manned by
representatives from USGS (week 1), DEQ/WPCD and EPA Region VI
(both study weeks).
C. Laboratory Support:
Laboratory support for this project will be provided by the various
agencies as listed below:
* Effluent analysis for specific chemicals will be provided by EPA
Region VI Houston Laboratory for all samples during both primary
study weeks and any followup effluent analyses that may be
necessary.
* ERL-N will be responsible for toxicity testing of all sediment
and ambient water samples and for definitive effluent testing of
PPG 001 and VISTA 001 during study weeks 1 and 2. As necessary,
additional definitive effluent testing will be undertaken by
ERL-N if indicated by preproject screening (6/6/88) or by DEQ
testing of other effluents during weeks 1 and 2.
* DEQ/WPCD Biotoxicity laboratory will be responsible for toxicity
testing of all project effluents during weeks 1 and 2 using the
Sheepshead Minnow test (Method 1004).
* USGS Denver Laboratory and USGS contract laboratories, Tennessee
Valley Authority and Mississippi State University, will be
responsible for specific chemical analyses of sediments and
ambient waters collected during study week 1.
- 24 -
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* EPA Region VI Houston Lab will be responsible for specific
chemical analyses of sediments collected during study week 2.
* DEQ/WPCD Laboratory will be responsible for specific chemical
analyses of ambient water samples collected during study week 2.
Responsible individuals for the respective agency laboratories are:
* EPA Region VI Houston Laboratory - Diana Ayres (6E-H),
(713) 526-1766
* EPA ERL-N - George Morrison, (401) 782-5000
* DEQ/WPCD Biotoxicity Laboratory - Teresa Jackson, (504) 295-8994
* DEQ/WPCD Water Quality/Organic Analyses Laboratories - Elaine
Sorbet, (504) 765-2405
* USGS, Water Resources Division, Louisiana District - Charles
Demas, (504) 389-0391
D. Documentation, Data Reduction, Reporting and Validation:
Field data and observations are to be documented using the DEQ/WPCD
Biological Survey Form (Exhibit 1). Copies of the Biological Survey
Forms are to be provided to each participating laboratory. In
addition, data and observations from these forms will be compiled
and reduced for use in preparation of the final project report.
Each respective project laboratory is responsible for documenting,
validating and reporting their respective analytical data.
Laboratory data will be appropriately tabulated or otherwise
presented according to field sample number, internal laboratory
tracking number and identified as to sampling location, date and
time. Participating laboratories will report analytical
data/results via their respective agency protocols for internal
agency review and approval. Agency approved and finalized
laboratory data are to be available by October 1988 for distribution
to project participants for review. Copies of validated laboratory
data and results are to be routed specifically to the following for
project report preparation:
(1) Kirk Manuel Calcasieu Field Project Officer
Louisiana Department of Environmental Quality
Water Pollution Control Division
Southwest Regional Office
1155 Ryan Street
Lake Charles, Louisiana 70602
(2) Michael Schurtz Louisiana Department of Environmental Quality
Office of Water Resources
Water Pollution Control Division
P.O. Box 44091
Baton Rouge, Louisiana 70804-4091
- 25 -
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(3) Philip Crocker U.S. Environmental Protection Agency
Allied Bank Tower
1445 Ross Avenue
Dallas, Texas 75202
E. Project Data Evaluation and Report Preparation:
Final project report preparation will be the responsibility of the
DEQ/WPCD Standards and Water Quality Management Section. All
participating agencies are encouraged to provide input to data
evaluation and report preparation. Narrative interpretation and
evaluation of the toxicity results will be provided by ERL-N.
Specific review and input from the Water Quality Management and
Permits Branches of the EPA Region VI Water Management Division will
be included in the report. All field and laboratory data of
acceptable quality will be assessed and discussed in this project
report. A draft final report will be available by the end of
January 1989 for review and revision by participating agencies. The
final report will be completed, incorporating comments and
revisions, by the end of March 1989.
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