Blackstone River Initiative
Phase 1: Dry Weather Assessment
Interim Report of Data 1991
APPENDICES
Prepared by:
The U.S. EPA Region 1 and the
Massachusetts Division of Water Pollution Control
in cooperation with the
Rhode Island Dept. of Environmental Management
and The University of Rhode Island
-------
Date of request:
CALL. NO.
!-)
-------
Table of Contents
1991 BLACKSTONE RIVER SURVEY
Phase 1: Dry Weather Assessment
.Appendix A Chemistry and Toxicity of Dischargers
.Appendix B Data Tables and Concentration Profiles
Appendix C Quality Assurance/Quality Control
Appendix D Bio Methods
Appendix E Bio Taxa
Appendix F Sediment Oxygen Demand
-------
Appendix A
1991BLACKSTONE RIVER SURVEY
CHEMISTRY AND TOXICITY OF DISCHARGERS
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4086 - Chronic Study VSlo/ZCgSTl-K. fiKllStfffiJi
SAS 1109
Leicesigyc m
L INTRODUCTION
Facility: Region t NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc. _ JUL ^ ^91.
2520 Regency Road q K-f
Lexington, Kentucky 40503-2961 '
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/03/91/7:25AM; 06/05/91/7:45AM; 06/07/91/12:25AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/04/91 (6:30PM); 06/04/91 (6:00PM)
Date and Time Test Terminated: 06/12/91 (7:15PM); 06/11/91 (5:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/06/91 (1:00PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCg, (96 hrs) = 6587 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS w (t
KPC.C- C- > I I jo L
State Permit Limits: N/A; NOEL: N/A ' £?VJC"^
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for the Ceriodaphnia dubia was 6.25% effluent concentration. The NOEC for the fathead minnow was 50%
effluent concentration. The LOEC, ECM, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXJCrTY TEST
METHOD: EPA 600(4*91001
Cerhdaphnla dubla Pimophalas promotes
Method: 1002.0 Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25" ± 1°C
25s ±1'C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
illumination (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx. 100 ft-c
5.
Photoperiod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 mi
7.
Test Solution Volume:
15 ml/replicate
200 ml/replicate
a
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with D.O. greater
than 100% saturation were
aerated
Samples with D.O. greater than 100%
than 100% saturation were aerated
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%. 25%. 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of the
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
in the control concentration
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 3, 5, and 7, 1991
EPA NO: 4086
Survival Data Analysis
Test
Organism
Sample
ID
Test
Concentration
<*)
% Survival by Days
3 4 5 6 7
Tox. Method
Reproduction Data Analysis
Average f
Young
Produced
Tox.
Method
Ceriodaphnla
dubla
Control
...
100
100
100
100
100
100
100
100
...
...
25
—
...
Final Effluent
6.25
100
100
100
100
100
90
90
90
No
Inspection
37
No
Inspection
12.5
100
100
100
100
100
100
100
100
No
Inspection
11
Yes
Dunnetfs
25
100
100
100
100
100
100
100
100
No
Inspection
6
Yes
Dunnett's
50
100
100
100
80
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
100
0
0
0
0
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding:
NOEC = 6.25% Effluent Concentration,
LOEC = 12.5% Effluent Concentration,
ChV s 8.8% Effluent Concentration,
ECn ¦ 35% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Test
Concentration
(*)
1
2
% Survival by Days
3 4 S 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
—
100
100
100 97 93 87
60
...
...
0.29
...
...
Minnow
Final Effluent
6.25
100
100
100 100 97 93
83
No
Inspection
0.36
No
Inspection
12.5
100
100
100 100 100 100
97
No
Inspection
0.36
No
Inspection
25
100
100
97 97 97 97
97
No
Inspection
0.34
No
Inspection
50
100
100
100 97 90 90
90
No
Inspection
0.25
No
Dunnetfs
100
63
37
10 10 3 0
0
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC « 50% Effluent Concentration,
LOEC a 100% Effluent Concentration,'
ChV c 71% Effluent Concentration,
ECh ¦ 65%Effluent Concentration,
Toxicity
-------
Blackstone River Survey
Worcester Finishing, Leicester, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
Collection Dates
PARAMETERS
06/03/91
06/05/91
06/09/91
Al
1.1
0.95
1.0
Cd
ND
0.005
0.009
Ca
1.6
2.1
4.8
Cr
ND
ND
ND
Cu
0.775
0.700
0.900
Pb
0.001
0.001
0.002
Mg
2.5
2.8
2.8
Ni
ND
ND
0.02
Zn
0.082
0.067
0.086
N-NH4
0.66
0.54
0.760
TS
5,800
5,900
6,000D
6,100
TSS
39
32
3 ID
30
TOC
76
73
89
Alk.(as mg/1 CaCO^)
340
350
350
NOTES: D= Sample Duplicate TOC= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Lexington, Kentucky
Environmental and Natural Resources Consulting and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
Facility: Region I
Contact: Ms. Cindy Schreyer
L INTRODUCTION
BIOMONITORING JOB NO: 4006/EPA 4090 - Chron
SAS 1109
NPDES Permit No: N/A
Study \Jhjo Si^XUld PUUifLfi
• Wr0rtzcUY~ « MA ^
Phone No: (703)519-1386
6*
Receiving Stream: N/A
Contact: Biilie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/03/91/9:30AM; 06/04/91/11:45PM; 06/06/91/11:00PM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen greater than 100
percent saturation, after warming, were aerated slowly until D.O. was below 100%.
Date and Time Test Started: 06/04/91 (7:15PM); 06/04/91 (7:30PM)
Date and Time Test Terminated: 06/12/91 (5:55PM); 06/11/91 (6:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600f4-89{001
Carhdaphnla dubta
Method: 1002.0
Pimophates promelas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
Illumination (cool
white)
Approx 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25® ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia\ Pimephales promelas
Age: Less than 24 hours; 1 day
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/06/91 (1:00PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 6176 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
KCCC-'C- L CSOr
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for the Ceriodaphnia dubia was <6.25% effluent concentration. The NOEC for the fathead minnow was
50% effluent concentration. The LOEC, ECgo, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BtOMONITORING RESULTS
SAMPLES COLLECTED JUNE 3, 4, and 6, 1991
EPA NO: 4090
Sufvlval Data Analysis Reproduction Data Analysis
Teet
Organism
Sample
ID
Test
Concentration
(%)
1
2
% Survival by Daye
3 4 6 6
7
8
Tox.
Method
Average 1
Young
Produced
Tox.
Metho
Cerlodaphnia
Control
...
100
100
100 100 100 100
100
100
...
N/A
...
...
dubla
Final Effluent
6.25
100
70
70 70 70 70
70
60
Yes
Fisher's
N/A
N/A
N/A
12.5
100
0
0 0 0 0
0
0
Yes
Inspection
N/A
N/A
N/A
25
100
0
0 0 0 0
0
0
Yes
Inspection
N/A
N/A
N/A
50
0
0
0 0 0 0
0
0
Yes
Inspection
N/A
N/A
N/A
100
0
0
0 0 0 0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC < 6.25% Effluent Concentration,
LOEC > 6.25% Effluent Concentration,
ChV > 6.25% Effluent Concentration,
ECh < 6.25% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Test
Concentration
(%)
1
2
% Survival by Days
3 4 6 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
100 100
93
87
87
...
...
0.29
...
...
Minnow
Final Effluent
6.25
100
100
100 97
97
93
93
No
Inspection
0.26
No
Inspection
12.5
100
100
97 97
97
93
93
No
Inspection
0.27
No
Inspection
25
100
97
97 97
97
93
97
No
Inspection
0.36
No
Inspection
50
100
100
100 90
90
87
87
No
Inspection
0.37
No
Inspection
100
67
20
17 17
17
17
17
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC » 60% Effluent Concentration,
LOEC « 100% Effluent Concentration,
ChV >71% Effluent Concentration,
ECW> 100% Effluent Concentration,
Toxicity
-------
Blackstone River Survey
New England Plating, Worcester, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 06/03/91 06/04/91 06/06/91
Al
0.02
0.05
0.01D
ND
cd
0.017
ND
0.005D
ND
Ca
320
140
140D
120
Cr
0.021
ND
NDD
ND
Cu
0.080
0.083
0.078D
0.065
Pb
0.001
0.001
NDD
ND
Mg
0.98
2.2
2.2D
0.065
Ni
0.44
0.23
0.25D
0.22
Zn
0.743
0.757
0.789D
0.871
N-NH4
8.1
12
7.4
TS
2500
3,200
2,700
TSS
4.0
5.7
7.0
T0C
31
23
23
Alk.(as CaCOj)
110
110
90
NOTES: D= Sample Duplicate
TS= Total Solids
ND= Below minimum detectable
T0C=
Alk.:
level TSS=
: Total Organic Carbons
= Alkalinity
: Total Suspensed Solids
-------
Edward C. Foree, Ph.D., P.E.
<%>
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky
Louisville, Kentucky ^ p/\ O
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4089 - Chronic Study
SAS1109
upper fy'gJtnu lAJP&b
Facility: Region I
L INTRODUCTION
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703) 519-1386
Receiving Stream: N/A
Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/03/91/857AM; 06/05/91/8:15AM; 06/07/91/12:30AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen readings greater
than 100 percent saturation, after warming, were aerated slowly until D.O. was below 100%.
Date and Time Test Started: 06/04/91 (7:30PM); 06/04/91 (7:00PM)
Date and Time Test Terminated: 06/12/91 (6:33PM); 06/11/91 (6:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 60014-89(001
Ceriodaphnta dubla
Method: 1002.0
Plmephales promotes
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25*±1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25°± 1°C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%. 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephales promelas
Age: Less than 24 hours; 1 day
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUAUTY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/06/91 (1:00PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/1 NaCI
Pimephales promelas - LCM (96 hrs) = 6587 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: No toxicity was indicated with the Ceriodaphnia dubia or the fathead minnow. The NOEC
values for the Ceriodaphnia dubia and the fathead minnow were >100% effluent concentration. The LOEC,
ECjo, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 3, 5, and 7, 1991
EPA NO: 4089
Teat
Organism
Sample
ID
Suivh/al Data Analysis
Reproduction Data Analysis
Tast
Concentration
<%>
% Survival by Days
3 4 8 6 7
Avaraga f
Tox.
Produced
Malhod YoungTox.tahri
Ceriodaphnia
dubla
Control
...
100
100
100
100
100
100
100
100
...
...
25
...
...
Final Effluent
6.25
100
90
90
80
eo
80
80
80
No
Inspection
20
No
Inspection
12.5
100
100
100
100
100
100
100
100
No
Inspection
30
No
Inspection
25
100
100
100
90
90
90
90
90
No
Inspection
23
No
Inspection
50
100
90
90
90
90
90
80
80
No
Inspection
21
No
Inspection
100
90
90
90
60
80
80
80
70
No
Inspection
18
No
Dunnett's
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV = 100% Effluent Concentration,
EC„ > 100% Effluent Concentration,
No Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Teat
Concentration
<%)
1
2
% Survival by Days
3 4 5 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
97
97
97
97
97
90
90
...
...
0.26
...
...
Minnow
Final Effluent
6.25
97
97
97
97
97
93
90
No
Inspection
0.19
No
Inspection
12.5
93
93
93
93
93
93
90
No
Inspection
0.25
No
Inspection
25
97
97
90
90
90
90
90
No
Inspection
0.19
No
Inspection
50
100
100
97
93
93
93
90
No
Inspection
0.16
No
Inspection
100
97
93
93
93
93
90
90
No
Inspection
0.25
No
Dunnett's
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV : 100% Effluent Concentration,
EC„ > 100% Effluent Concentration,
No Toxicity
-------
Edward G. Forec, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Lexington, Kentucky
Environmental and Natural Resources Consulting and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4189 - Chronic Study
SAS 1109
Vpp6rbUci2.sk>/it
WAbfPoflvte&t- MbaXtrxJ/Ct-
?>ishtcb
L INTRODUCTION
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703) 519-1386
Receiving Stream: N/A
Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/6:55AM; 08/07/91/5:40AM; 08/09/91/5:40AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 08/06/91 (4:00PM); 08/06/91 (4:00PM)
Date and Time Test Terminated: 08/13/91 (420PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25" ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXICITY 7EST
METHOD: EPA 600/4^9/001
Carbdsphnla dubla
Method: 1002.0
PImtphales promelas
Method: 1000.0
1.
Test type:
Static-Renewal
Static-Renewal
2.
Temperature:
25*±1#C
25'± 1*C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
illumination (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx. 1001t-c
5.
Photoperlod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
15 ml/replicate
200 ml/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp naupiii
In a concentrated suspension
twice daily.
13.
Aeration:
Samples with D.O. greater
than 100% saturation were
aerated
Samples with D.O. greater
than 100% saturation were
aerated
14.
Dilution Water
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
In the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia-, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2261 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 50% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, EC^, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4189
Survival Data Analysis Reproduction Data Analysts
Test
Sample
Teet
% Survival by Days
Average *
Organism
ID
Concentration
1
2
3
4
8
6
7
Tox.
Method
Young
TOX.
Method
<%)
Produced
Certodaphnla Control
—
100
100
100
100
100
100
100
...
...
22
—
—
dubta
Final Effluent
6.25
100
100
100
100
100
100
100
No
Inspection
34
No
Inspection
12.5
100
100
100
100
100
100
90
No
Inspection
29
No
Inspection
25
100
100
100
100
100
100
100
No
Inspection
30
No
Inspection
50
100
100
100
100
100
100
100
No
Inspection
28
No
Inspection
100
100
100
100
100
100
100
70
No
Inspection
14
Yes
Inspection
Finding:
NOEC b 80% Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV > 70.7% Effluent Concentration,
EC_ > 100% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Sample
Test
% Survival by Daya
Average Wt.
Organism
ID
Concentration
1
2
3
4
8
6
7
Tox.
Method
per Larvae
Tox.
Method
<*>
(mg)
Fathead
Control
...
100
100
100
97
97
90
90
...
—
0.42
—
...
Minnow
Final Effluent
6.25
100
100
100
93
93
93
93
No
Inspection
0.50
No
Inspection
12.5
100
100
100
93
93
93
93
No
Inspection
0.50
No
Inspection
25
100
100
100
97
97
97
97
No
Inspection
0.54
No
Inspection
50
100
100
97
93
93
93
93
No
Inspection
0.34
No
Dunnetfs
100
100
100
97
97
97
97
97
No
Inspection
0.34
No
Dunnetfs
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV b 100% Effluent Concentration,
EC- > 100%Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Upper Blackstone WPAD," Millbury, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 06/03/91 06/05/91 06/07/91 08/05/91 08/07/91 09/09/91
Al
0.05
0.05
0.08
0.043
0.039
<0.020
cd
ND
0.005
0.005
0.019
0.0044
0.022
Ca
19
19
26
19
20
21
Cr
ND
ND
ND
<0.0050
<0.0050
0.0050
Cu
0.038
0.043
0.048
0.037
0.041
0.036
Pb
0.005
. ND
0.004
0.0025
0.0033
0.0020
Mg
3.5
4.9
3.8
2.7
3.0
3.1
Ni
0.02
0.03
0.03
0.042
0.067
0.17
Zn
0.041
0.054
0.070
0.12
0.12
0.10
N-NH4
1.3
0.72
0.72D
0.77
0.26
0.34
0.16
TS
300
320
370
300
320
340
TSS
4.0
5.0
6.0
<5
<5
<5
TOC
8.9
9.1
8.2
8.1
11
8.8
Alk.(mg/1 CaCO;)
40
36
36
31
39
30
Notes:
ND= Below minimum detectable level(MDL)
D= Sample Duplicate Alk.= Alkalinity
TS= Total Solids TSS= Total Suspensed Solids
T0C= Total Organic Carbons
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
Lexington, Kentucky
COMMONWEALTH TECHNOLOGY, INC
Environmental and Natural Resources Consulting and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4088 - Chronic
SAS 1109
L INTRODUCTION
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703) 519-1386
Receiving Stream: N/A
Contact: Billie Rogers
qxJas of JUL 4
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/03/91/9:03AM; 06/05/91/8:50AM; 06/07/91/10:20AM
Collection Method: Grab
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and contra! was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen (D.O.) greater than
100 percent saturation, after warming to 25°C, were aerated slowly until D.O. was less than 100%.
Date and Time Test Started: 06/04/91 (7:30PM); 06/04/91 (7.00PM)
Date and Time Test Terminated: 06/12/91 (7:00PM); 06/11/91 (6:34PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer io Table 1
Test Temperature (mean and range): Cultuied at test temperature, 25° + 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXJCrTY TEST
METHOD: EPA 600f4~89/001
Carfodaphnta dubla
Method: 1002.0
Plmaphales promotes
Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25'± 1"C
25'i 1*C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
IllUhiinalion (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx. 100 ft-c
5.
Photoperiod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
15 ml/replicate
200 ml/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with O.O. greater
than 100% saturation were
aerated
Samples with D.O. greater
than 100% saturation were
aerated
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%. 25%. 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of the
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia:, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight Of applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/06/91 (1:00PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 6587 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. The NOEC was 50% effluent
concentration. No toxicity was indicated with the fathead minnow. The NOEC was >100% effluent
concentration. The LOEC, ECM, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 3, 5, and 7, 1991
EPA NO: 4088
Survival Data Analysis
Reproduction Data Analysis
Test
Orgenlsin
Sample
ID
Test
Concentration
(%)
1
2
% Survival by Deye
3 4 8 6
7
8
Tox.
Method
Average 1
Young
Produced
Tox.
Method
Ceriodaphnia
Control
...
100
100
100 100 100 100
100
100
...
...
25
...
...
dubta
Final Effluent
6.25
100
100
100 100 100 100
90
90
No
Inspection
40
No
Inspection
12.5
100
100
100 100 100 100
100
90
No
Inspection
38
No
Inspection
25
100
100
100 100 100 100
100
90
No
Inspection
38
No
Inspection
50
100
100
100 100 100 100
go
90
No
Inspection
31
N/A
Inspection
100
100
100
100 100 80 30
30
30
Yes
Fisher's
N/A
N/A
N/A
Finding: NOEC a 80% Effluent Concentration,
LOEC a 100% Effluent Concentration,
ChV = 71% Effluent Concentration,
ECn b 82% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Test
Concentration
<%)
1
2
% Survival by Days
3 4 8 6
7
Tox.
Method
Averege Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
•••
100
100
100 97 93
87
80
...
...
0.29
•"
...
Minnow
Final Effluent
6.25
100
100
97 97 97
93
90
No
Inspection
0.25
No
Inspection
12.5
100
100
100 100 100
97
97
No
Inspection
0.23
No
Inspection
25
100
100
100 100 97
90
90
No
Inspection
0.21
No
Inspection
50
100
97
93 93 90
83
83
No
Inspection
0.29
No
Inspection
100
100
100
80 70 67
60
60
No
Dunnett's
0.28
No
Dunnetfa
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV s 100% Effluent Concentration,
ECM > 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Millbury WWTP, Millbury, tfa.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 06/03/91 06/05/91 06/07/91
Al
0.13
0.15
0.14
Cd
ND
ND
0.004
Ca
18
16
18
Cr
ND
ND
ND
Cu
0.088
0.083
0.078
Pb
0.011
0.006
0.004
Mg
3.3
3.0
3.3
Ni
ND
ND
0.01
Zn
0.056
0.144
0.109
N-NH4
4.0
39
20
TS
400
500
510
TSS
14
19
21
T0C
28
35
34
Alk. (as CaCOj)
62
90
90D
64
NOTES: D= Sample Duplicate
TS= Total Solids
ND= Below minimum detectable level
T0C= Total Organic Carbons
TSS= Total Suspensed Solids
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Lexington, Kentucky
Environmental end Natural Resources Consulting end Analytical Services
Louisville, Kentucky
Grayson, Kentucky
1. INTRODUCTION
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703)519-1386
Receiving Stream: N/A
Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/03/91/9:35AM; 06/05/91/10:45AM; 06/07/91/11:10AM
Collection Method: Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
111. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen (D.O.) greater than
100 percent saturation after warming, were aerated slowly until D.O. was below 100%.
Date and Time Test Started: 06/04/91 (730PM); 06/04/91 (6:30PM)
Date and Time Test Terminated: 06/12/91 (6:45PM); 06/11/91 (5:30PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperaiure. 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICTTY TEST
METHOD: EPA 600/4^9/001
Carhdaphnla dubla
Method: 1002.0
Plmaphalas promolas
Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25'±1°C
2S*±1*C
3.
Light Quality:
Ambient laboratory
illumination (cool
white}
Ambient laboratory -
illumination (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx. 100 ft-c
5.
Photoperiod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
15 ml/replicate
200 ml/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber:
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Salenastrum mihutum
(algae} suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with D.O. greater
than 100% saturation were
aerated
Samples with D.O. greater
than 100% saturation were
aerated
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia\ Pimephales prometas
Age: Less than 24 hours; 2 days
Source: Stock N; 'Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/06/91 (1:00PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/I NaCI
Pimephales prometas • LC*, (96 hrs) = 6587 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. The NOEC for the Ceriodaphnia dubia
was 25% effluent concentration. No toxicity was indicated with the fathead minnow. The NOEC was
>100% effluent concentration. The LOEC, ECM, and Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 3, 5, and 7, 1991
EPA NO: 4087
Survival Data Analysis
Reproduction Data Analysis
Test
Organism
Sample
ID
Test
Concentration
(*)
1
2
% Survival by Days
3 4 8 6
7
8
ToX.
Method
Averege 1
Young
Produced
To*.
Method
Ceriodaphnia
Control
...
100
100
100
100
100
100
100
100
•••
25
...
...
dubia
Final Effluent
6.25
100
90
00
70
70
70
70
70
No
Fisher's
19
No
Ounnetfs
12.5
100
100
90
70
70
70
70
70
No
Inspection
15
No
Dunnett's
25
100
100
90
90
90
90
90
90
No
Inspection
18
No
Dunnett's
SO
100
80
70
60
60
60
60
60
No
Inspection
6
Yes
Dunnett's
100
100
100
100
SO
70
70
70
70
No
Fisher's
3
Yes
Inspection
Finding: NOEC a 25% Effluent Concentration,
LOEC = 50% Effluent Concentration,
ChV s 35% Effluent Concentration,
ECW> 100% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Test
Concentration
(%>
.1
2
% Survival by Daya
3 4 5 6
T
To*.
Method
Average Wt.
per Larvae
(mg)
To*.
Method
Fathead
Control
...
100
100
100
97
93
87
80
...
...
0.29
...
...
Minnow
Final Effluent
6.25
100
100
100
97
87
63
77
No
Inspection
0.34
No
Inspection
12.5
100
100
100
93
93
93
90
No
Inspection
0.33
No
Inspection
25
100
100
100
97
83
80
BO
No
Inspection
0.30
No
Inspection
50
100
100
100
100
97
93
87
No
Inspection
0.28
No
Inspection
100
100
100
100
97
97
80
77
No
Dunnett's
0.27
No
Dunnett's
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV a 100% Effluent Concentration,
ECn> 100% Effluent Concentration,
No Toxicity
-------
Blacks tone River Survey
Grafton VJWTP, Grafton, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unles otherwise noted)
Collection Dates
PARAMETERS
06/03/91
06/05/91
06/07/91
Al
0.10
0.13
0.28
Cd
ND
0.003
0.004
Ca
16
16
17
Cr
ND
ND
ND
Cu
0.018
0.033
0.035
Pb
0.004
ND
0.001
Mg
3.5
3.5
3.0
Ni
0.01
ND
ND
Zn
0.049
0.050
0.058
N-NH4
1.5
1.5
3.0
TS
390
370
400
TSS
4.8
6.6
20
TOC
11
13
16
12D
Alk.(as CaCOj) 64 70 78
NOTES: D= Sample Duplicate
TS= Total Solids
ND= Below minimum detectable level
T0C= Total Organic Carbons
TSS= Total Suspensed Solids
-------
Edward G. Force, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4190 ¦ Chronic Study
SAS1109 tiotLTH&VbCfZ f07Wt H4-
L INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
SAMPLES {EFFLUENT AND DILUTION WATER)
Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/7:37AM; 08/07/9l/6:35AM; O8/09/91/6:0OAM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
II.
1.
2.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001,
Deviations from methods, If 8ny, and explanation: After warming the samples to 25'C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%. The samples were dechlorinated with sodium thiosulfate because their chlorine
concentration was >0.02 mg/L
Date and Time Test Started: 08/06/91 (3:15PM); 08/06/91 (3:45PM)
Date and Time Test Terminated: 08/13/91 (3:30PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25" ± 1°C
-------
TABLE 1
SUMMARY OF 7EST CONDTTIONS FOR CHRONIC TOXJCfTY TEST
METHOD: EPA 600/4-89001
C«rtodaphnla dubla Pfmaphalas promelas
Method: 1002.0 Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25* ± 1*C
25° ± 1*C
3.
Light Quality:
Ambient laboratory
(Rumination (cod
white)
Ambient laboratory
Illumination (cool
white)
4.
Light Intensity:
Appro*. tOO ft-c
Approx. 100 ft-c
5.
Photoperiod:
8 hrs. dark, 16 hrs.
fight
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 mt
7.
Test Solution Volume:
15 mi/replicate
200 ml/repiicata
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime;
Fed YCT (yeast suspension)
& Sefarastrum minutum
(algae) suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with O.O. greater
than 100% saturation were
aerated
Samples with D.O. greater
than 100% saturation were
aerated
14.
Dilution Water.
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
625%
100%, 50%, 25%, 12.5%,
6.25%
te.
Test Duration:
7 days or until €0% of
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
ia.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
In the control concentration
'
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia:, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2261 mg/I NaCI
Pimephales promelas - LC^ (96 hrs) = 7227 mg/L NaCt
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 6.25% effluent concentration. The NOEC
for the fathead minnow was >100% effluent concentration. The LOEC, EC^,, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUSTS, 7, AND 9, 1991
EPA NO: 4190
Teet
Organism
Sample
ID
Survival Data Analysis
Reproduction Data Analysis
Test
Concantratlon
<*)
% Survival by Days
3 4 S e 7
To*. Mathod
Average I
Young
Produced
To*.
Method
CetkxiapMa
dub/a
Control
—
100
100
100
100
100
100
100
...
...
22
...
—
Final Effluent
6.25
100
100
100
100
100
100
100
No
Inspection
17
No
T-Tost
12.5
100
100
100
100
100
100
100
No
Inspection
13
Yes
T-Test
25
100
100
100
100
100
100
go
No
Inspection
B
Yas
T-Test
50
100
100
00
80
80
80
80
No
Inspection
11
Yes
T-Test
100
100
100
00
B0
so
00
80
No
Inspection
11
Yes
T-Test
Finding: NOEC- 625% Effluent Concentration,
LOEC ¦ 12.5% Effluent Concentration,
CtiV o 8.8% Efltuant Concantratlon,
ECa> 100% Effluant Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organlem
Sample
ID
Teat
Concentration
(*)
1
2
% Survival by Days
3 4 5 6
7
Tox.
Method
Average Wt.
per Larvae
(rng)
Tox.
Method
Fathead
Control
...
100
100
100 97 97 90
90
...
...
0.42
...
—
Minnow
Final Effluent
6.2S
100
100
100 100 100 100
100
No
Inspection
0.53
No
Inspection
12.5
97
97
97 93 93 93
93
No
Inspection
0.52
No
Inspection
25
100
100
100 100 100 100
100
No
Inspection
0.49
No
Inspection
50
100
100
97 93 90 90
90
No
Inspection
0.46
No
Inspection
100
100
100
100 100 100 100
100
Yes
Inspection
0.46
No
Inspection
finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
ECn> 100% Effluent Concentration,
No Toxicity
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp. Ph.D., P.E., L.S
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BtOMONITORING JOB NO: 4005/EPA 4191 - Chronic Study
SAS 1109 (/X3&2>45 AtfW, HA
L INTRODUCTION
Facility: Region! NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1336
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/8:20AM; 08/07/91/7:55AM; 08/09/91/7:05AM
Collection Method: 24 Hour Composte
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25SC the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 08/06/91 (4:00PM); 08/06/91 (3:50PM)
Date and Time Test Terminated: 08/13/91 (4:00PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Teat Temperature (mean and range): Cultured at test temperature, 25* ± 1®C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600/4-89/001
Cariodaphnla dubla
Method: 1002.0
Plmaphales promalas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperlod:
6. Test Chamt)^ Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 mi/replicate.
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%. 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25*±rC
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 mi/replicate
Daily
1-2 days
10
3
Fed brine shrimp naupiii
In a concentrated suspension
twice daily.
Samples wittr D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
In the control concentration
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC. JUL i 8 1991
Environmental and Natural Resources Consulting and Analytical Services .
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
§UtLFOCb /Albc/sre/
, HA-
BiOMONITORING JOB NO: 4006/EPA 4093 - Chronic Study
SAS 1109
L INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703)519-1386
Receiving Stream: N/A Contact: BilEie Rogers
Laboratory: Comrr wealth Technology, Inc.
2520 i agency Read
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Efftuent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/8:45AM; 06/19/91/7:50AM; 06/21/91/7:50AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, H any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen p.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/18/91 (2:10PM); 06/18/91 (2:30PM)
Date and Time Test Terminated: 06/26/91 (2:20PM); 06/25/91 (2:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXJCTTY TEST
METHOD: EPA 600f4-89f001
Cariodaphnla dubia
Method: 1002.0
Plmephalms promalas
Method: J000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per-Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
15. Test Acceptability:
Static-Renewal
25* ± 1"C
Ambient laboratory
Illumination (cool
white)
Approx. 100 ft-c
8 hrs. daik, 16 hrs.
light
30 mis
15 ml/repiicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(aigae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6^5%
7 days or until 60% of Ihe
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25*±rC
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. da/k, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia1, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LC^, (48 hrs) = 2261 mg/1 NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL* N/A
Test Results: No toxicity was indicated with the Ceriodaphnia dubia or the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was >100% effluent concentration. The LOEC,
ECjo, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendbc A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUSTS, 7, AND 9, 1991
EPA NO: 4191
Survival Data Analysis
Reproduction Data Analysis
Test
Orgenlem
Sample
ID
Teat
Concentration
<*)
1
2
% Survival by Days
3 4 8 8
T
To*.
Method
Average 1
Young
Produced
To*.
Method
Certodaphnla
Control
...
00
00
00 00
go so
go
...
...
22
...
•—
dub/a
Final Effluent
6.2B
100
100
ioo go
go go
go
No
Inspection
22
No
Inspection
12.6
00
00
00 90
go go
go
No
Inspection
26
No
Inspection
2B
100
100
100 100
100 100
100
No
Inspection
27
No
Inspection
60
00
90
00 00
00 90
go
No
Inspection
25
No
Inspection
100
KM
100
100 100
100 100
100
No
Inspection
19
No
Inspection
Finding: NOEC > 100% Effluent Concentration,
LOEC >100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
EC„ > 100% Effluent Concentration,
No Toxicity
Survival Oala Analysis
Growth Data Analysis
Teet
Orgenlem
Sample Teet
10 Concentration
(*)
1
2
% Survival by Oaya
3 4 8 6
7
To*.
Method
Average Wt.
per Larvae
(mg)
To*.
Method
Fathead
Control
100
100
100
100
100
100
100
...
...
0.36
...
...
Minnow
Find Effluent 6.25
100
100
100
100
100
100
100
No
Inspection
0.39
No
Inspection
12.5
100
100
100
93
90
go
90
No
Inspection
0.50
No
Inspection
25
100
100
100
100
07
97
97
No
Inspection
0.46
No
Inspection
50
100
100
100
100
100
97
97
No
Inspection
0.47
No
Inspection
100
100
100
100
100
100
100
100
No
Inspection
0.39
No
Inspection
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV b 100% Effluent Concentration,
ECn> 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Uxbridge VJWTF, Uxbridge, Ma
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite sampls
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMLTEKS
08/05/91
08/07/91
08/09/91
Al
0.041
0.024
0.047
Cd
<0.0030
<0.0030
<0.0030
Ca
15
15
16
Cr
<0.0050
<0.0050
<0.0050
Cu
0.052
0.067
0.067
Pb
0.0028
0.0020
0.0020
Mg
2.3
2.2
2.4
Ni
0.012
0.019
0.012
Zn
0.11
0.19
0.13
N-NH4
0.32
0.15
0.22
TS
320
340
330
TSS
10
5
<5
TOC
13
11
12
Alk.(mg/1 as CaOOj) 13
8
16
NOTES: D= Sample Duplicate
T0C=
Total Organic Carbons
TS=
Total Solids
Alk.:
: Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia• Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia • LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 50% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, ECjo, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, AND 21, 1991
EPA NO: 4093
Survival Data Analysis Reproduction Data Analysis
Test Sample Teat % Sutvfval by Days Average I
Organism ID Concentration 12346676 Tox. Method Young Tox. Method
(*) Produced
Certodaphnla Control
dubla
Final Effluent 6.25
12.5
25
60
100
Finding: NOEC a BOX Effluent Concsntratlon,
LOEC ¦ 100% Effluent Concentration,
ChV > 70.7% Effluent Concentration,
EC,,* 100% Effluent Concentration,
Toxicity
100
100
100
80
80
80
80
80
...
100
100
go
90
B0
80
80
80
No
100
100
100
100
so
go
90
00
No
100
100
100
100
100
100
100
100
No
100
100
100
00
80
80
80
80
No
100
100
100
100
100
100
100
80
No
...
30
...
...
Inspection
42
No
Inspection
Inspefilon
44
No
Inspection
Inspection
41
No
Inspection
Inspection
23
No
Inspection
Inspection
3
Yes
Inspection
Survival Data Analysis
Growth Data Analysis
Test
Orgenlsm
Sample
ID
Test
Concentration
<*>
1
2
% Survival by Days
3 4 5 6
7
Tox.
Method
Average Wl.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
93
87
87
87
87
80
•••
0.42
...
...
Minnow
Final Effluent
6.25
100
97
97
93
93
93
93
No
Inspection
0.43
No
Inspection
12.5
83
83
80
80
73
73
70
No
Inspection
0.61
No
Inspection
25
97
97
97
97
87
87
80
No
Inspection
0.48
No
Inspection
50
97
97
97
97
97
97
97
No
Inspection
0.50
N(J
Inspection
100
87
83
80
77
77
73
73
No
Dunnett's
0.49
No
Inspection
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV a 100% Effluent Concentration,
EC„> 100%Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Guilford Industries, East Douglas, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS
06/17/91
06/19/91
06/21/91
Al
0.02
0.02
0.04
Cd
ND
ND
0.007
Ca
5.5
5.7
5.6
Cr
ND
0.004
0.011
Cu
0.005
ND
ND
Pb
ND
ND
ND
Mg
1.3
1.3
1.2
Ni
ND
ND
ND
Zn
0.003
0.294
0.315
N-NH4
0.16
0.09
0.11
TS
280
280
250
TSS
12
11
13
TOC
51
48
44
Alk. (as CaCO,)
38
34
34
NOTES: D= Sample Duplicate TOC= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
Edward G. Force, Ph.D., P.E. ¦J-o' John S. Tapp, Ph.D., P.E., L.S
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4192 - Chronic Study
SAS1109 ~2>0V4L+£ fcfW, MAr
I. INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Biilie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2951
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/9:00AM; 08/07/91 /8:55AM; 08/09/91/8:30AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, If any, and explanation: None Performed.
Date and Time Test Started: 08/06/91 (2:00PM); 08/06/91 (2:00PM)
Date and Time Test Terminated: 08/13/91 (2:30PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1*C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600/4S9/001
Cariodaphnla dubla
Method: 1002.0
Plmaphalas promalas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperlod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25*± 1"C
Ambient laboratory
Illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 his.
light
30 mis
15 mi/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Setenastrum minutum
(algae) suspension daily.
None
Reconstituted water (10%
Perrier water)
100%, 50%. 25%. 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25'jtrC
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 mi/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daiiy.
None
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia:, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2261 mg/I NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated wrth the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 25% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, ECM, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4193
Suivlval Data Analysis Reproduction Data Analysis
Test
Sample Test
% Survival by Daya
Average t
Orgsnlsm
ID Concentration
1
2
a
4
5
6
7
Tox.
Method
Young
Tox.
Method
(%)
Produced
Ceriodaphnla Control
100
100
100
100
100
100
100
...
...
22
...
...
dubla
Final Effluent 6.25
100
100
90
90
70
60
60
No
Inspection
15
No
Inspection
12.5
100
100
100
100
100
90
90
No
Inspection
23
No
Inspection
25
100
100
100
100
100
70
70
No
Inspection
18
No
Inspection
50
100
100
100
100
0
0
0
Yes
Inspection
N/A
N/A
N/A
100
100
100
100
0
0
0
0
Ves
Inspection
N/A
N/A
N/A
Finding:
NOEC u 25% Effluent Concentration,
LOEC a 50% Effluent Concentration,
ChV b 35.4% Effluent Concentration,
ECn = 29% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Tast
Sample Test
% Survival by Days
Average Wt.
Orgsnlsm
ID Concentration
1
2
3
4
5
6
7
Tox.
Method
per Larvae
Tox.
Method
(*)
(mg)
Fathead
Control
' 100
100
97
97
97
97
97
...
...
0.45
...
—
Minnow
Final Effluent 6.25
100
100
100
100
100
100
100
No
Inspection
0.52
No
Inspection
12.5
07
97
97
93
93
93
93
No
Inspection
0.52
No
Inspection
25
100
100
100
100
100
100
100
No
Inspection
0.43
No.
Inspection
50
100
100
100
93
93
93
93
No
Inspection
0.52
No
Inspection
100
100
100
97
93
93
90
90
No
Inspection
0.46
No
Inspection
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV s 100% Effluent Concentration,
EC„> 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Douglas WVfTP, East Douglas, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite 8/5/91 and 8/9/91
Grab sample collected on 8/7/91
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 08/05/91 08/07/91 (G) 08/09/91
Al
0.98
0.10
0.11
Cd
<0.0030
<0.0030
<0.0030
Ca
18
17
16
Cr
<0.0050
<0.0050
<0.0050
Cu
0.23
0.046
0.039
Pb
0.020
0.0034
0.039
Mg
3.0
2.1
1.9
Ni
<0.0050
0.016
<0.0050
Zn
0.20
0.10
0.079
N-NH4
31
29
25
TS
560
370
380
TSS
240
27
35
TOC
35
22
32
Alk.(as CaCOj)
230
80
210
NOTES: D= Sample Duplicate TOC= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
X- ppm Ca003 (G>= Grab samples Taken
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp. Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
jui 18 m
Lexington, Kentucky
Environmental and Natural Resources Consuitint and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4094 - Chronic Study
SAS 1109
WoWScUET fold, Rl
L INTRODUCTION
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703) 519-1386
Receiving Stream: N/A
Contact Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
11. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/7:20AM; 06/19/91/9:20AM; 05/21/91/9:20AM
Collection Method: Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used lor dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
HI. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the ChronicToxicity of Effluents and Receiving Waters
to Freshwater Organisms* EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen (D.O.) greater than
100 percent saturation after warming, were aerated slowly until D.O. was below 100%.
Date and Time Test Started: 06/18/91 (2:15PM); 06/1B/91 (3:00PM)
Date and Time Test Terminated: 06i26/91 (2:30PM); 06/25/91 (3:30PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600/4-891001
Carhdaphnia dubla
Method: 1002.0
Plmaphatas pmmalas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photo period:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber:
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25° i 1*C
Ambient laboratory
flluminaiion (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/repficate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastnim nvnutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25* ±1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 mi
200 mi/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephaies promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable}: N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephaies promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2S45 mg/l NaCI
Pimephaies promelas - LCM (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was 50% effluent concentration. The LOEC, ECM,
and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4094
Survival Data Analysis Reproduction Data Analysis
Test Sample Teal % Survival by Daya Average *
Organlam ID Concentration 12346676 Tox. Method Young To*. Method
(%) Produced
Certodaphnla
dubla
Control
...
100
100
100
80
ao
ao
80
60
...
...
30
...
...
Final Effluent
6.25
100
100
100
100
100
100
100
100
No
Inspection
51
No
Inspection
12.S
100
100
100
100
100
100
100
100
No
Inspection
39
No
Inspection
25
100
100
100
100
100
100
100
100
No
Inspection
47
No
Inspection
50
100
100
100
100
100
100
00
00
No
Inspection
32
No
Inspection
100
70
20
20
20
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC « 80% Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentration,
EC„ • 66% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Teat
Organism
Sample
ID
Test
Concentration
<*)
% Survival by Daya
3 4 6 6 7
Tox. Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Minnow
Control
Final Effluent
...
100
93
67
87
67
67
80
...
...
0.42
...
...
6.25
100
100
100
97
07
93
93
No
Inspection
0.46
No
Dunnetfs
12.5
97
93
93
93
93
93
93
No
Inspection
0.38
No
Dunnetfs
25
87
87
87
87
87
87
87
No
Inspection
0.36
No
Dunnetfs
50
90
87
87
87
83
83
83
No
Inspection
0.39
No
Dunnetfs
100
87
27
10
0
0
0
0
Yea
Inspection
N/A
N/A
N/A
Flnitlng: NOEC ¦ 60% Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentration,
EC„ ¦» 63% Effluent Concentration,
Toxicity
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resource Consulting and Analytical Services
Lexington, Kentucky
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4193 - Chronic Study
SAS 1109
\poOh)St>o;&T Pc>tWi tl
L INTRODUCTION
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703)519-1386
Receiving Stream: N/A
Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/10:00AM: 08/07/91/10:30AM; 08/09/91/9:55AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 08/06/91 (3:00PM); 08/06/91 (4:00PM)
Date and Time Test Terminated: 08/13/91 (2:30PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 18C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600f4-89f001
Cerlodaphnla dubla
Method: 1002.0
PImephalas promelas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light intensity:
5. Photoperlod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%. 12^%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25'±1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 mi
200 ml/replicate
Daiiy
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia', Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (If applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCW (48 hrs) = 2261 mg/l NaCl
Pimephales promelas - LCs, (96 hrs) = 7227 mg/L NaCl
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 25% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, EC,,,, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4193
Survival Data Analysis Reproduction Data Analysts
Test Sample Test % Survival by Days Averaga t
Organism ID Concantrallon 12 3 4 5 6 7 Tox. Method Young Tox. Method
(%) Produced
Certodaphnla Control
dubla
Final Ellluanl 6.2S
12.5
25
50
100
Finding: NOEC ¦ 25% Effluent Concentration,
LOEC ¦ 50% Effluent Concantrallon,
ChV ¦ 35.4% Effluent Concentration,
ECn > 29% Effluent Concentration,
Toxicity
100 100 100 100 100 100 100
100
100
00
00
70
60
60
No
100
100
100
100
100
00
00
No
100
100
100
100
100
70
70
No
100
100
100
100
0
0
0
Yes
100
100
100
0
0
0
0
Yes
...
22
...
...
Inspection
15
No
Inspection
Inspection
23
No
Inspection
Inspection
16
No
Inspection
Inspection
N/A
N/A
N/A
Inspection
N/A
N/A
N/A
Survival Data Analysis
Growth Data Analysis
Teat
Organism
Sample
ID
Teal
Concentration
(*)
% Survival by Day*
3 4 6 6 7
Tox. Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Minnow
Control
...
100
100
07
97
97
97
97
...
...
0.45
...
...
Final Effluent
6.25
100
100
100
100
100
100
100
No
Inspection
0.52
No
Inspection
12.5
07
07
07
S3
93
93
93
No
Inspection
0.52
No
Inspection
25
100
100
100
100
100
100
100
No
Inspection
0.43
No.
Inspection
50
100
100
100
03
93
93
03
No
Inspection
0.52
No
Inspection
100
100
100
07
93
93
90
90
No
Inspection
0.46
No
Inspection
Finding: N0EC> 100% Effluent Concentration,
LOEC > 100% Effluent Concantrallon,
ChV = 100% Effluent Concentration,
EC„> 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Woonsocket FOIV, Woonsocket, RI
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 06/17/91 06/19/91 06/21/91 08/05/91 08/07/91 08/09/91
Al
0.20
0.15
0.22
0.15
0.20
0.13
Cd
ND
0.005
0.004
<0.0030
0.0059
0.0038
Ca
23
17
18
20
18
19
Cr
ND
ND
ND
<0.0050
0.015
0.0086
Cu
0.085
0.043
0.065
0.044
0.075
0.52
Pb
0.004
0.005
0.009
0.0090
0.015
0.0089
Mg
3.0
2.5
3.3
3.7
3.3
3.4
Ni
ND
0.01
0.01
0.0065
0.030
0.0089
Zn
0.039
0.092
0.106
0.097
0.140
0.16
N-NH4
20
14
15
13
7.5
7.9
TS
540
870
900
450
890
830
TSS
15
10
32
58
37
24
T0C
28
40
54
18
44
34
Alk.(mg/1 CaCOj)
130
130
150
92
110
120
Notes: ND= Below minimum detectable level
D= Sample Duplicate Alk.= Alkalinity
TS= Total Solids TSS= Total Suspensed Solids
T0C= total Organic Carbons
-------
Edward G. Foree, Ph.D., P.E.
Lexington, Kentucky
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC. J(JJ_ j[ g ]Qg]
Environmental end Natural Resources Consulting and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4095 • Chronic Study
SAS 1109
L INTRODUCTION
Facility: Region I
Contact: Ms. Cindy Schreyer
Receiving Stream: N/A
NPDES Permit No: N/A
Phone No: (703)519-1306
Contact: Billie Rogers
OKbtJfTB
Laboratory: Commanwsafth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(€06) 275-3505
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/11:45AM; 06/19/91/11:20AM; 06/21/91/11:20AM
Collection Method: 24 Hour Composte
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
•less than 100%. The samples were dechlorinated with sodium thiosulfate because their chlorine
concentration was >0.02 mg/L
Dateand Time Test Started: 06/18/91 (2:00PM); 06/18/91 (2:30PM)
Date and Time Test Terminated: 06/26/91 (2:15PM); 06/25/91 (3:00PM)
Test.Chambers: Refer to Table 1
Volume used per Chamber Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDrTJONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 60014-89(001
Cwhdaphnla dubia
Method: 1002.0
Plmaphalms promelas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Fhotoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of the
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25*±rc
Ambient laboratory
fflumtnation (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCjo (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LC^ (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: No toxicity was indicated with the Ceriodaphnia dubia or the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was >100% effluent concentration. The LOEC,
EC*,, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4095
Suivfval Data Analysis Reproduction Data Analysis
Tost Sample Taat % Survival by Days Avorags #
Organism ID Concentration 1234B87B To*. Method Young Tox. Method
(%) Produced
Ceriodophnla
dubla
Control
...
100
100
100
90
90
90
90
90
...
...
25
—
—
Final Effluent
6.25
100"
100
100
80
60
60
60
60
No
Fisher's
17
No
Inspection
12.5
100
100
100
100
70
60
60
60
No
Fisher's
16
No
Inspection
25
100
100
100
100
90
80
80
70
No
Inspection
16
No
Inspection
50
100
100
100
100
89
89
89
56
No
Fisher's
19
No
Inspection
100
100
100
100
100
80
80
80
70
No
Inspection
32
No
Dunnetfs
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
EC,, > 100% Efflusnt Concentration,
No Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Orgsnlsm
Sample
ID
Test
Concentration
(%)
% Survival by Days
3 4 S 6 7
Tox. Method
Average Wt.
par Larvae
(mg)
Tox.
Method
Fathead
Minnow
Control
Final Effluent
100 100 87 93 93 90 90
0.51
6.25
97
93
90
90
83
83
83
No
Dunnetfs
0.49
No
Dunnetfs
12.5
100
97
97
97
97
93
93
No
Dunnetfs
0.44
No
Dunnetfs
25
100
100
100
100
100
93
93
No
Dunnetfs
0.46
No
Dunnetfs
50
97
93
90
90
90
90
90
No
Dunnetfs
0.51
No
Dunnetfs
100
97
93
93
93
90
90
90
No
Dunnetfs
0.46
No
Dunnetfs
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV = 100% Effluent Concentration,
ECW > 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Okonite Industries, Ashton, RI
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS
06/17/91
06/19/91
06/21/91
Al
0.08
0.04
0.10
Cd
ND
ND
ND
Ca
14
13
12
Cr
0.007
ND
ND
Cu
0.018
0.015
0.020
Pb
ND
0.002
0.010
Mg
2.8
2.8
2.3
Ni
ND
ND
ND
Zn
0.058
0.073
0.108
N-NH4
ND
ND
ND
TS
200
150
140
TSS
ND
ND
ND
TOC
15
2.2
2.7
Alk. (as CaCO.)
34
44
28
NOTES: D= Sample Duplicate TOC= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
Edward G. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
JUL 18 1991
Lexington, Kentucky
Environmental and Natural Resources Consulting end Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMON1TORING JOB NO: 4006/EPA 4096 - Chronic Study
SAS 1109
L INTRODUCTION
- cot A
Facility: Region I
NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer
Phone No: (703) 519-1386
Receiving Stream: N/A
Contact: Biltie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/2:00PM; 06/19/91/12:30AM; 06/21/91/12:30AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8,
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms* EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25eC the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/18/91 (2:00PM); 06/18/91 (2:00PM)
Date and Time Test Terminated: 06/26/91 (9:50AM); 06/25/91 (2:15PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± l°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600/4*9/001
Ceriodaphnla dubla
Method: 1002.0
PImephalas promotes
Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25*±1*C
25* ± 1*C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
illumination (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx 100 ft-c
5.
Photoperiod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
IS ml/replicate
200 ml/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber.
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with D.O. greater
than 100% saturation were
aerated.
Samples with D.O. greater
than 100% saturation were
aerated.
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia\ Pimephates promeias
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephates promeias 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCl
Pimephates promeias • LC^ (96 hrs) = 6295 mg/L NaCl
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for the Ceriodaphnia dubia was 6.25% effluent concentration. The NOEC for the fathead minnow was
12.5% effluent concentration. The LOEC, EC*,, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4096
Survival Data Analysis
Reproduction Data Analysis
Test
Organism
Sample
ID
Test
Concentration
<*)
1
2
% Survival by Days
3 4 8 8
7
9
Average I
Tox.
Produced
Method
YoungTox.l
Ceriodaphnla
Control
...
100
100
100
90
90
90
90
90
...
...
37
...
...
dubla
Final Effluent
6.25
90
00
90
90
60
80
80
80
No
Inspection
34
No
Inspection
12.3
100
100
100
100
60
90
90
90
No
Inspection
17
Yea
Inspection
25
100
50
40
40
40
40
40
40
Yes
Fisher's
N/A
N/A
N/A
60
30
0
0
0
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
100
0
0
0
0
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC ¦ 655* Effluent Concentration,
LOEC a 12.6% Effluent Concentration,
ChV ¦ 8.8% Effluent Concentration,
ECa >18% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Teat
Concentration
<*)
1
2
% Survival by Daya
3 4 8 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
97
93
93
90
90
—
•••
0.51
...
Minnow
Final Effluent
6.25
100
93
93
93
93
90
90
No
Inspection
0.40
No
Dunnstfs
12.8
93
93
93
93
93
93
90
No
Inspection
0.37
No
Dunnetfs
28
37
27
13
13
13
7
7
Yes
Inspection
N/A
N/A
N/A
50
0
0
0
0
0
0
0
Yea
Inspection
N/A
N/A
N/A
100
0
0
0
0
0
0
0
Yea
Inspection
N/A
N/A
N/A
Finding: NOEC ¦ 12.8% Effluent Concentration,
LOEC * 28% Effluent Concentration,
ChV ¦ 17.7% Effluent Concentration,
EC„ ¦ 17% Effluent Concentration,
Toxicity
-------
Blackstone River Survey
GTE, Central Falls, RI
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
Outfall: Outfall 001A Outfall 00IB
PARAMETERS 06/17/91 06/19/91 06/21/91 06/17/91 06/19/91 06/21/91
Al
0.68
0.53
1.2
0.08
0.10
0.08
Cd
NT)
ND
ND
ND
ND
ND
Ca
140
40
42
7.6
7.6
7.4
Cr
0.069
0.073
0.238
0.005
ND
ND
Cu
0.028
0.008
0.028
ND
ND
ND
Pb
0.004
0.012
0.008
0.012
0.046
0.032
Mg
0.28
0.10
0.450
1.5
1.5
1.6
Ni
ND
ND
ND
ND
ND
ND
Zn
ND
0.009
0.073
0.162
0.169
0.185
N-NH4
190
110
280
ND
ND
ND
TS
3,100
2,900
4,500
330
110
110
TSS
43
10
120
6.8
9.0
13
TOC
33
38
30
8.4
5.5
4.0
Alk. (as CaCQ-)
J
46
72
90
18
19
19
NOTES: D= Sample Duplicate T0C= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
Edward G. Force, Ph.D., P.E. VU^ John S. Tapp, Ph.D.. P.E.. L.S.
COMMONWEALTH TECHNOLOGY, INC. j\JL 18 1991
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Ksniuclcy
BIOMONITORING JOB NO: 4006/EPA 4097 - Chronic Study
SAS 1109
£7"2T - OOf &
I INTRODUCTION CS^JT^L FfrUS , /fX
Facility. Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth TechnoJogy, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/3:30PM; 06/19/91/1:40PM; 06/21/91/1:40PM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating th j Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25'C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/18/91 (3:00PM); 06/18/91 (4:30PM)
Date and Time Test Terminated: 06/26/91 (3:10PM); 06/25/91 (5:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600(4-89(001
Cerlodaphnla dubla
Method: 1002.0
Plmephales promelas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber:
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Appro x. 100 ft-c
8 hrs. dark, 16 hrs.
tight
30 mis
15 mi/repiicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Sarrples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Pem'er water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or unfit 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25" ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/repticate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Pemer water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia: Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LC^ (48 hrs) = 2846 mg/I NaCI
Pimephales promelas - LCM (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
-tate Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and fathead minnow was 50% effluent concentration. The LOEC, EC^,
and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BtOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4097
Survival Data Analysis
Reproduction Data Analysis
Teat
Organlem
Sample
ID
Teet
Concentration
(%)
1
2
% Survival by Daya
3 4 8 6
7
8
Tox.
Method
Average I
Young
Produced
Tox.
Method
Cerlodaphnla
Control
...
100
100
80 80 80
80
80
80
...
...
16
...
...
dubla
Final Effluent
6.25
100
100
100 100 100
100
80
80
No
Inspection
16
No
Inspection
12.S
100
100
100 100 100
100
100
100
No
Inspection
16
No
Inspection
25
100
100
100 100 100
100
100
100
No
Inspection
15
No
Inspection
50
100
100
100 00 90
90
90
90
No
Inspection
22
No
Inspection
100
100
100
90 90 80
70
50
SO
No
Fisher's
5
Yes
Inspection
Finding: NOEC ¦ 50* Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV « 70.7% Effluent Concentration,
ECm ¦ 100% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Teat
Orgenlsm
Sample
ID
Test
Concentration
(*)
1
2
% Survival by Days
3 4 6 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
100
100
97
97
97
97
93
...
...
0.44
—
...
Minnow
Final Effluent
6.28
97
97
93
93
93
93
90
No
Dunnetfs
0.44
No
Dunnetfs
12.6
97
97
97
93
90
90
87
No
Dunnetfs
0.47
No
Dunnetfs
25
97
97
97
97
97
97
97
No
Dunnetfs
0.48
No
Dunnetfs
50
80
77
73
73
73
73
73
No
Dunnetfs
0.35
No
Dunnetfs
100
67
17
10
10
10
10
10
Yes
Dunnetfs
N/A
N/A
N/A
Finding: NOEC a BOX Effluent Concentration,
LOEC b 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentration,
ECn ° 04% Effluent Concentration,
Toxicity
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp. Ph.D.. P.E.. L.S
COMMONWEALTH TECHNOLOGY, INC.
Environmental end Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONfTORING JOB NO: 4006/EPA 4191 - Chronic Study
SAS1109 OXB&l>4g fovS, HA-
L INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703)519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 0B/05/91/8:20AM; 08/07/91/7:55AM; 08/09/91/7:05AM
Collection Method: 24 Hour Composte
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, If any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 08/06/91 (4:00PM); 08/06/91 (3:50PM)
Date and Time Test Terminated: 08/13/91 (4:00PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDrTJONS FOR CHRONIC TOXICrTY TEST
METHOD: EPA 600f4-89{001
Carlodaphnla dubla
Method: 1002.0
PImaphalas promalas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperlod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
2r±rc
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. da/k, 16 hrs.
light
30 mis
15 mi/repiicaie
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum rrdnutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Penrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25*±1'C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 mi/replicate
Daily
1-2 days
10
3
Fed brine shrimp naupiii
in a concentrated suspension
twice daily.
Samples wittr D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
Edward G. Foree, Ph.D., P.E. J°hn S" TaPP' Ph'D" P'E" L'S'
COMMONWEALTH TECHNOLOGY, INC. JUL 18 1991
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4093 - Chronic Study
SAS 1109
§UICFC£6 /NOUSTlZiVS
L INTRODUCTION , M4-
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Comn wealth Technology, Inc.
2520 i ..-gency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/8:45AM; 06/19/91/7:50AM; 06/21/91/7:50AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/18/91 (2:10PM); 06/18/91 (2:30PM)
Date and Time Test Terminated: 06/26/91 (250PM); 06/25/91 (2:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC
METHOD: EPA 600(4-89(001
Cwkxtaphnla dubla
Method: 1002.0
TOXICITY TEST
Plmaphalas promalas
Method: 4000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per-Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25,±i*c
Ambient laboratory
illumination (oool
white)
Approx 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/repiicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of the
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia1, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCU (48 hrs) = 2261 mg/I NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: No toxicity was indicated with the Ceriodaphnia dubia or the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was >100% effluent concentration. The LOEC,
EC,,,, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4191
Survival Data Analysis Reproduction Data Analysis
Teat
Sampla
Test
% Survival by Daya
Average f
Organism
ID
Concentration
1
2
3
4
5
B
7
Tox.
Method
Young
Tox.
Method
(%)
Produced
Certodaphnla Control
—
00
00
00
00
00
00
00
...
...
22
...
...
dubta
Final Effluent
6.28
100
100
100
00
00
00
00
No
Inspection
22
No
Inspection
12.S
90
00
B0
90
00
90
00
No
Inspection
26
No
Inspection
26
100
100
100
100
100
100
100
No
Inspection
27
No
Inspection
60
00
90
00
90
00
90
90
No
Inspection
25
No
Inspection
100
-MX)
100
100
100
100
100
100
No
Inspection
19
No
Inspection
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
EC_ > 100% Effluent Concentration,
No Toxicity
Survival Data Analysis
Growth Data Analysis
Tast
Sample
Teal
% Survival by Daya
Averege Wl.
Organism
ID
Concentration
1
2
a
4
s
6
7
Tox.
Method
per Larvae
Tox.
Method
(*)
(mg)
Fathead
Control
...
100
100
100
100
100
100
100
...
•••
0.36
—
—
Minnow
Final Effluent
6.25
100
100
100
100
100
100
100
No
Inspocllon
0.39
No
Inspection
12.5
100
100
100
03
00
90
00
No
Inspection
0.50
No
Inspection
25
100
100
100
100
07
97
97
No
Inspection
0.48
No
Inspection
50
100
100
100
100
100
97
97
No
Inspection
0.47
No
Inspection
100
100
100
100
100
100
100
100
No
Inspection
0.39
No
Inspection
Finding:
NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV a 100% Effluent Concentration,
EC_ > 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Uxbridge WTF, Uxbridge, Ma
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite sainpls
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS
08/05/91
08/07/91
08/09/91
Al
0.041
0.024
0.047
Cd
<0.0030
<0.0030
<0.0030
Ca
15
15
16
Cr
<0.0050
<0.0050
<0.0050
Cu
0.052
0.067
0.067
Pb
0.0028
0.0020
0.0020
Mg
2.3
2.2
2.4
Ni
0.012
0.019
0.012
Zn
0.11
0.19
0.13
N-NH4
0.32
0.15
0.22
TS
320
340
330
TSS
10
5
<5
TOC
13
11
12
Alk.(mg/1 as CaCOj) 13
8
16
NOTES: D= Sample Duplicate
T0C=
Total Organic Carbons
TS=
Total Solids
Alk. =
: Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia-, Pimephales prometas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 05/12/91 (5:30PM)
Pimephales prometas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales prometas - LC^ (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 50% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, ECm, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, AND 21, 1991
EPA NO: 4093
Suivlval Data Analysis Reproduction Data Analysis
Test Sample Test % Survival by Days Average 0
Organism 10 Concentration 1 2 3 4 6 6 7 6 Tox. Method Young Tox. Method
(%) Produced
Ceriodaphnla Control
dubla
Final Effluent
...
100
100
100
eo
ao
6.25
100
100
so
00
B0
12.5
100
100
100
100
so
25
100
100
100
100
100
B0
100
100
100
go
eo
100
100
100
100
100
100
eo
80
80
...
...
eo
80
eo
No
Inspection
so
00
so
No
Inspe^lon
100
100
100
No
Inspection
80
80
80
No
Inspection
100
100
80
No
Inspection
30
...
...
42
No
Inspection
44
No
Inspection
41
No
Inspection
23
No
Inspection
3
Yes
Inspection
Finding: NOEC • BO* Effluent Concentration,
LOEC a 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentration,
ECn > 100% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organlam
Sample
ID
Teal
Concentration
<*)
% Survival by Days
3 4 S 6 7
Tox. Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Minnow
Control
...
100
S3
67
87
67
87
60
...
...
0.42
...
...
Final Effluent
6.25
100
87
87
83
83
83
83
No
Inspection
0.43
No
Inspection
12.5
83
83
eo
80
73
73
70
No
Inspection
0.61
No
Inspection
25
87
67
87
07
67
87
80
No
Inspection
0.48
No
Inspection
50
87
87
B7
87
87
87
87
No
Inspection
0.50
Nd
Inspection
100
87
83
80
77
77
73
73
No
Dunnett's
0.4S
No
Inspection
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
EC„ > 100%Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Guilford Industries, East Douglas, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS
06/17/91
06/19/91
06/21/91
Al
0.02
0.02
0.04
Cd
ND
ND
0.007
Ca
5.5
5.7
5.6
Cr
ND
0.004
0.011
Cu
0.005
ND
ND
Pb
ND
ND
ND
Mg
1.3
1.3
1.2
Ni
ND
ND
ND
Zn
0.003
0.294
0.315
N-NH4
0.16
0.09
0.11
TS
280
280
250
TSS
12
11
13
TOC
51
48
44
Alk. (as CaCOj)
38
34
34
NOTES: D= Sample Duplicate TOC= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
-------
Edwerd G. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S
COMMONWEALTH TECHNOLOGY, INC.
Environmental end Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4192 - Chronic Study
SAS 1109 ~2>OVf)Uh5>
I. INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1385
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(506) 275-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/9:00AM; 08/07/91/8:55AM; 08/09/91/8:30AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms" EPA/600/4-89/001.
Deviations from methods, if any, and explanation: None Performed.
Date and Time Test Started: 08/06/91 (2:00PM); 08/06/91 (2:00PM)
Date and Time Test Terminated: 08/13/91 (2:30PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25s ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 60014-89/001
Carlodaphnla dubla
Method: 1002.0
Plmephalas promalas
Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25* ± 1*C
2S>t 1*C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
illumination (cool
white)
4.
Light Intensity:
Approx. 100 ft-c
Approx. 100 ft-c
5.
Photoperlod:
8 hrs. dark, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
15 ml/replicate
200 mi/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp naupiii
in a concentrated suspension
twice daily.
13.
Aeration:
None
None
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
In the control concentration
-------
N. TEST ORGANISMS
Name: Ceriodaphnia dubia:, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCH (48 hrs) = 2261 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for the Ceriodaphnia dubia was 25% effluent concentration. The NOEC for the fathead minnow was 50%
effluent concentration. The LOEC, ECM, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORtNG RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4192
Survival Date Analysis
Reproduction Data Analysis
Teet
Organism
Sample
IO
Test
Concentration
(%)
1
2
% Survival by Daya
3 4 S fl
7
To*.
Method
Average 1
Young
Produced
To*.
Method
Caiodapttnla
Control
...
90
BO
90
BO
60
BO
90
...
—
22
...
...
dubia
Final ENuertt
0,25
100
100
100
100.
100
100
100
No
Inspection
28
No
Inspection
12.6
100
too
100
100
100
100
100
No
Inspection
30
No
Inspection
25
100
100
100
100
100
100
100
No
Inspection
21
No
Inspection
60
SO
80
80
80
80
80
80
No
Inspection
10
Yes
Inspection
100
70
30
20
20
10
10
0
Yea
Inspection
N/A
N/A
N/A
Finding: NOEC =25* Efflusnl Concentration,
LOEC ¦ 50% Effluent Concentration,
ChV s 35.4% Effluent Concentration,
EC„ ^ 62% Effluent Concentration,
Toxicity
Survival Oala Analysis
Growth Data Analysis
Test
Organism
Sample
10
Teet
Concentration
(*>
1
2
% Survival by Days
3 4 8 6
T
Tox.
Method
Average Wl.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
100 100 100 100
100
...
...
0.36
—
...
Minnow
Final Effluent
6.25
100
100
100 100 100 100
100
No
Inspection
0.38
No
Inspection
12.6
100
100
100 100 100 100
100
No
Inspection
0.47
No
Inspection
25
100
03
83 S3 90 BO
60
No
Inspection
0.42
No
Inspection
SO
97
97
97 97 97 97
97
No
Inspection
0.32
No
Inspection
100
0
0
0 0 0 0
0
Ybs
Inspection
N/A
N/A
N/A
Finding: NOEC « SOX Effluent Concentration,
LOEC a 100% Effluent Concentration,
ChV b 70.7% Etltuant Concentration,
EC„ b 68% Effluent Concentration,
Toxicity
-------
Blackstone River Survey
Douglas VfWTP, East Douglas, Ma.
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite 8/5/91 and 8/9/91
Grab sample collected on 8/7/91
ANALYTICAL RESULTS
{mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 08/05/91 08/07/9KG) 08/09/91
Al
0.98
0.10
0.11
Cd
<0.0030
<0.0030
<0.0030
Ca
18
17
16
Cr
<0.0050
<0.0050
<0.0050
Cu
0.23
0.046
0.039
Pb
0.020
0.0034
0.039
Mg
3.0
2.1
1.9
Ni
<0.0050
0.016
<0.0050
Zn
0.20
0.10
0.079
N-NH4
31
29
25
TS
560
370
380
TSS
240
27
35
TOC
35
22
32
Alk. (as CaCOj)
230
80
210
NOTES: D= Sample Duplicate T0C= Total Organic Carbons
TS= Total Solids Alk.= Alkalinity
ND= Below minimum detectable level TSS= Total Suspensed Solids
t~ ppin CaC03 (G)= Grab samples Taken
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC. jyL 1 8 •
Environmental end Natural Resources Consulting end Analytical Servicer
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4094 - Chronic Study
SAS1109 , . , /?_-
I. INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Biilie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/720AM; 05/19/91/9:20AM; 05/21/91/9:20AM
Collection Method: Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms* EPA/600/4-89/001.
Deviations from methods, if any, and explanation: Samples with dissolved oxygen (D.O.) greater than
100 percent saturation after warming, were aerated slowly until D.O. was below 100%.
Date and Time Test Started: 06/18/91 (2:15PM); 06/18/91 (3:00PM)
Date and Time Test Terminated: 06/26/91 (2:30PM); 06/25/91 (3:30PM)
Test Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1#C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXSCnY TEST
METHOD: EPA 6004-89(001
Carhdaphnla dubia
Method: 1002.0
Plmophalas promotes
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperlod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber.
11. ' No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25*41*0
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
fight
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Setenastmm mnutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
In the control concentration
Static-Renewal
25* ±1*0
Ambient laboratory
illumination (cool
white)
Approx 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 mi/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
In the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia • LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LC^ (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was 50% effluent concentration. The LOEC, ECjo,
and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4094
Survival Data Analysis
Test
Organism
Sample
ID
Test
Concentration
(*)
% Survival by Dtyi
3 4 6 8 7
Tox. Method
Reproduction Data Analysis
Average t
Young
Produced
To*.
Method
Certodaphnle
dubta
Control
...
100
100
100
80
80
80
80
80
...
...
30
...
...
Final Effluent
6.2S
100
100
100
100
100
100
100
100
No
Inspection
51
No
Inspection
12.5
100
100
100
100
100
100
100
100
No
Inspection
30
No
Inspection
25
100
100
100
100
100
100
100
100
No
Inspection
47
No
Inspection
50
100
100
100
100
100
100
00
00
No
Inspection
32
No
Inspection
100
70
20
20
20
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding: NOEC ¦ 80% Effluent Concentration,
LOEC m 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentretlon,
EC„ ¦ 68% Effluent Concentration,
Toxicity
Suivlval Data Analysis
Test Sample Teat % Survival by Daya
Organism ID Concentration 1 2 3 4 6 6 7 Tox. Method
<*)
Growth Data Analysis
Average Wt.
per Laivee Tox. Method
(mg)
Fathead
Minnow
Control
Final Effluent
...
100
93
87
87
87
87
80
...
...
0.42
...
...
6.25
100
100
too
97
97
93
93
No
Inspection
0.46
No
Dunnett's
12.5
#7
93
93
93
93
93
93
No
Inspection
0.38
No
Dunnetfs
25
87
87
87
87
67
67
87
No
Inspection
0.36
No
Dunnett's
50
90
87
87
67
63
83
83
No
Inspection
0.39
Nop
Dunnetfs
100
87
27
10
0
0
0
0
Yea
Inspection
N/A
N/A
N/A
Finding:
NOEC ¦ 60% Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV ¦ 70.7% Effluent Concentration,
ECm • 63% Effluent Concentration,
Toxicity
-------
Edward G. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S
COMMONWEALTH TECHNOLOGY, INC.
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4153 - Chronic Study
SAS 1109
wooHSocte-r PvtW, K.T
I. INTRODUCTION
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2961
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 08/05/91/10:00AM; 08/07/91/10:30AM; 08/09/91/9:55AM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms* EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen P.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 08/06/91 (3:00PM); 08/06/91 (4:00PM)
Date and Time Test Terminated: 08/13/91 (2:30PM); 08/13/91 (4:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 18C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 600/4-89/001
Carlodaphnla dubla
Method: 1002.0
Plmephalas promotes
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light intensity:
5. Photo period:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white}
Approx 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/repiicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%. 50%, 25%. 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25'±1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia\ Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 08/05/91 (1:00PM)
Pimephales promelas 08/06/91 (9:00AM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia • LCM (48 hrs) = 2261 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 7227 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with-the Ceriodaphnia dubia. No toxicity was indicated with the
fathead minnow. The NOEC for the Ceriodaphnia dubia was 25% effluent concentration. The NOEC for
the fathead minnow was >100% effluent concentration. The LOEC, EC*,, and the Chronic Values are
presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONtTORING RESULTS
SAMPLES COLLECTED AUGUST 5, 7, AND 9, 1991
EPA NO: 4193
Suivh/al Data Analysis
Reproduction Data Analysis
Teet
Sample
Test
% Survival by Days
Average •
Organism
ID
Concentration
1
2
3
4
5
8
7
Tox.
Method
Young
Tox.
Method
<%)
Produced
Cerkxiaphnla Control
...
100
100
100
100
100
100
100
— .
...
22
...
...
dubla
Final Effluent
6.25
100
100
00
90
70
60
60
No
Inspection
15
No
Inspection
12.5
100
100
100
100
100
SO
00
No
Inspection
23
No
Inspection
25
100
100
100
100
100
70
70
No
Inspection
18
No
Inspection
50
100
100
100
100
0
0
0
Y09
Inspection
N/A
N/A
N/A
100
100
100
100
0
0
0
0
Ves
Inspection
N/A
N/A
N/A
Finding:
NOEC ¦ 25% Effluent Concentration,
LOEC a 50% Effluent Concentration,
ChV ¦ 35.4% Effluent Concentration,
EC_ ¦ 29% Effluent Concentration,
Toxicity
Suivlval Data Analysis
Growth Dala Analysis
Test
Organism
Sample
ID
Test
Concentration
(*)
1
2
% Survival by Days
3 4 5 8
7
Tox.
Method
Averege Wt
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
97 97 97 97
97
...
...
0.45
...
Minnow
Final Effluent
6.25
100
100
100 100 100 100
100
No
Inspection
0.52
No
Inspection
12.5
97
97
97 93 93 93
93
No
Inspection
0.52
No
Inspection
25
100
100
100 100 100 100
100
No
Inspection
0.43
No.
Inspection
50
100
100
100 93 93 93
93
No
Inspection
0.52
No
Inspection
100
100
100
97 93 93 90
90
No
Inspection
0.46
No
Inspection
Finding: NOEC> 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV * 100% Effluent Concentretlon,
ECn > 100% Eftluenl Concentration,
No Toxicity
-------
Blackstone River Survey
Woonsocket POTW, Woonsocket, RI
CHEMICAL ANALYSIS OF TOXICITY' SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
{mg/1 unless otherwise noted)
Collection Dates
PARAMETERS 06/17/91 06/19/91 06/21/91 08/05/91 08/07/91 08/09/91
Al
0.20
0.15
0.22
0.15
0.20
0.13
Cd
ND
0.005
0.004
<0.0030
0.0059
0.0038
Ca
23
17
18
20
18
19
Cr
ND
ND
ND
<0.0050
0.015
0.0086
Cu
0.085
0.043
0.065
0.044
0.075
0.52
Pb
0.004
0.005
0,009
0.0090
0.015
0.0089
Mg
3.0
2.5
3.3
3.7
3.3
3.4
Ni
ND
0.01
0.01
0.0065
0.030
0.0089
Zn
0.039
0.092
0.106
0.097
0.140
0.16
N-NH4
20
14
15
13
7.5
7.9
TS
540
870
900
450
890
830
TSS
15
10
32
58
37
24
TOC
28
40
54
18
44
34
Alk.(mg/1 CaOOj)
130
130
150
92
110
120
Notes:
ND= Below minimum detectable
level
D= Sample Duplicate
Alk.=
Alkalinity
TS= Total Solids TSS= Total Suspensed Solids
T0C= Total Organic Carbons
-------
Edward G. Force, Ph.D., P.E.
John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC. JUL 1 8 1991
Environmental and Natural Resources Consulting and Analytical Service!
Lexington, Kentucky
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4095 - Chronic Study
SAS1109
I. INTRODUCTION
Facility: Region I
Contact: Ms. Cindy Schreyer
Receiving Stream: N/A
OKbtilTE
AS&foti ,£T
NPDES Permit No: N/A
Phone No: (703)519-1386
Contact: Billie Rogers
Laboratory: Commonwealth Technology, inc.
2520 Regency Road
Lexington, Kentucky 40503-2951
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/11:45AM; 06/19/91/11:20AM; 06/21/91/11:20AM
Collection Method: 24 Hour Ccmposte
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
IIL TEST METHODS
Test Method: "Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 25°C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated siowiy until D.O. was
• less than 100%. The samples were dechlorinated with sodium thiosulfate because their chlorine
concentration was >0.02 mg/L.
Date and Time Test Started: 06/18/91 (2:00PM); 06/18/91 (2:30PM)
Date and Time Test Terminated: 06/26/91 (2:15PM); 06/25/91 (3:00PM)
Test.Chambers: Refer to Table 1
Volume used per Chamber: Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° + 1°C
-------
TABLE 1
SUMMARY OF TEST CONDITIONS FOR CHRONIC TOXICITY 7EST
METHOD: EPA 600/4-89(001
Carbdaphnla dubia Plmmphalas promelas
Method: 1002.0 Method: 1000.0
1.
Test Type:
Static-Renewal
Static-Renewal
2.
Temperature:
25* ± 1*C
25"±1#C
3.
Light Quality:
Ambient laboratory
illumination (cool
white)
Ambient laboratory
illumination (cool
white)
A.
Light Intensity:
Approx. 100 ft-c
Approx. 100 ft-c
5.
Photoperiod:
8 hrs. daric, 16 hrs.
light
8 hrs. dark, 16 hrs.
light
6.
Test Chamber Size:
30 mis
270 ml
7.
Test Solution Volume:
15 ml/replicate
200 ml/replicate
8.
Renewal of Test Concentrations:
Daily
Daily
9.
Age of Test Organism:
Less than 24 hours
1-2 days
10.
No. of Test Organisms per Chamber
1
10
11.
No. of Replicate Chambers per Concentration:
10
3
12.
Feeding Regime:
Fed YCT (yeast suspension)
& Selenastrum minutum
(algae) suspension daily.
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
13.
Aeration:
Samples with D.O. greater
than 100% saturation were
aerated
Samples with D.O. greater
than 100% saturation were
aerated
14.
Dilution Water:
Reconstituted water (10%
Perrier water)
Reconstituted water (10%
Perrier water)
15.
Effluent Concentrations:
100%, 50%, 25%, 12.5%,
6.25%
100%, 50%, 25%, 12.5%,
6.25%
16.
Test Duration:
7 days or until 60% of the
the control females have 3
broods
7-days
17.
Effects Measured:
Survival and Reproduction
Survival and Growth
18.
Test Acceptability:
80% or greater survival
in the control concentration
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia\ Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCM (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: No toxicity was indicated with the Ceriodaphnia dubia or the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and the fathead minnow was >100% effluent concentration. The LOEC,
ECjo, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4095
Survival Data Analysis Reproduction Data Analysis
Test Sample Test % Survival by Days Average I
Organism ID Concentration 12348678 Tox. Method Young Tox. Method
(%) Produced
Cerlodaphnla
dubta
Control
...
100
100
100
90
00
go
90
90
...
...
25
...
...
Final Effluent
6.25
100
100
100
80
60
60
60
60
No
Fisher's
17
No
Inspection
12.5
100
100
100
100
70
60
60
60
No
Fisher's
16
No
Inspection
25
100
100
100
100
00
80
80
70
No
Inspection
16
No
Inspection
SO
100
100
100
100
80
89
89
56
No
Fisher's
19
No
Inspection
100
100
100
100
100
80
80
80
70
No
Inspection
32
No
Dunnetfe
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
ECm > 100% Effluent Concentration,
No Toxicity
Survival Dala Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Teal
Concentration
(%>
1
2
% Survival by Days
3 4 8 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
97 93 93
90
90
...
...
0.51
•••
...
Minnow
Final Effluent
6.25
97
93
90 90 83
83
83
No
Dunnetfs
0.49
No
Dunnetfe
12.5
100
97
97 97 97
93
93
No
Dunnetfs
0.44
No
Dunnetfs
25
100
100
100 100 100
93
93
No
Dunnetfe
0.46
No
Dunnetfe
50
97
93
90 90 90
90
90
No
Dunnetfs
0.51
No
Dunnetfe
100
97
93
93 93 90
90
90
No
Dunnetfs
0.46
No
Dunnetfs
Finding: NOEC > 100% Effluent Concentration,
LOEC > 100% Effluent Concentration,
ChV ¦ 100% Effluent Concentration,
EC„ > 100% Effluent Concentration,
No Toxicity
-------
Blackstone River Survey
Okonite Industries, Ash ton, RI
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
PARAMETERS
06/17/91
06/19/91
06/21/91
A1
0.08
0.04
0.10
Cd
ND
ND
ND
Ca
14
13
12
Cr
0.007
ND
ND
Cu
0.018
0.015
0.020
Pb
ND
0.002
0.010
Mg
2.8
2.8
2.3
Ni
ND
ND
ND
Zn
0.058
0.073
0.108
N-NH4
ND
ND
ND
TS
200
150
140
TSS
ND
ND
ND
TOC
15
2.2
2.7
Alk. (as CaOOj)
34
44
28
NOTES: D= Sample Duplicate
TS= Total Solids
ND= Below minimum detectable level
TOC= Total Organic Carbons
Alk.= Alkalinity
TSS= Total Suspensed Solids
-------
Edward C. Foree, Ph.D., P.E.
John S. Tapp, Ph.D., F.E., L.S.
COMMONWEALTH TECHNOLOGY, INC.
JUL 18 1991
Lexington, Kentucky
Environmental end Natural Resources Consulting and Analytical Services
Louisville, Kentucky
Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4096 - Chronic Study
SAS 1109
i. INTRODUCTION
§Tc ' OOtA
fZHjS,
-------
TABLE 1
SUMMARY OF TEST CONDrTlONS FOR CHRONIC TOXICITY TEST
METHOD: EPA 60014-69(001
Ceriodaphrtla dubla
Method: 1002.0
Plmephalts promelas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Ught Intensity:
5. Photoperiod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber.
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25° i 1°C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
30 mis
15 ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (/east suspension)
6 SeJenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
the control females have 3
broods
Survival and Reproduction
80% or greater survival
in the control concentration
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp naupiii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia; Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCM (48 hrs) = 2846 mg/l NaCI
Pimephales promelas - LCjo (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VL RESULTS
State Permit Limits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for the Ceriodaphnia dubia was 6.25% effluent concentration. The NOEC for the fathead minnow was
12.5% effluent concentration. The LOEC, ECm, and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4096
Suivlval Data Analysis
Reproduction Data Analysis
Teat
Organism
Sampla Test
ID Concentration
<%)
1
2
% Survival by Daya
3 4 5 8
r
8
Average 1
Tox.
Produced
Method
YoungTox.l
Coriodaphnla Control
100
100
100
SO
90
90
90
90
...
...
37
...
...
dubla
Final Effluent 6.25
SO
go
90
B0
80
80
80
80
No
Inspection
34
No
Inspection
12.5
100
100
100
100
90
SO
90
90
No
Inspection
17
Yes
Inspection
25
100
50
40
40
40
40
40
40
Yes
Fisher's
N/A
N/A
N/A
60
30
0
0
0
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
100
0
0
0
0
0
0
0
0
Yes
Inspection
N/A
N/A
N/A
Finding:
NOEC ¦ 6.25% Effluent Concentration,
LOEC s 12.6% Effluent Concentration,
ChV « 8.6% Effluent Concentration,
EC„ ¦ 18% Effluent Concentration,
Toxicity
Suivlval Data Analysis
Growth Data Analysis
Test
Sample Teat
% Survival by Diya
Average Wt.
Organism
ID Concentration
1
2
3
4
8
6
7
Tox.
Method
per Larvae
Tox.
Method
(*)
17.7% Effluent Concentration,
ECn ¦ 17% Effluent Concentration,
Toxicity
-------
Blackstone River Survey
C7TE, Central Falls, RI
CHEMICAL ANALYSIS OF TOXICITY SAMPLES
24-hr time-composite samples
Outfall:
PARAMETERS
ANALYTICAL RESULTS
(mg/1 unless otherwise noted)
Collection Dates
Outfall 001A Outfall 00IB
06/17/91 06/19/91 06/21/91 06/17/91 06/19/91 06/21/91
Al
0.68
0.53
1.2
0.08
0.10
0.08
Cd
ND
ND
ND
ND
ND
ND
Ca
140
40
42
7.6
7.6
7.4
Cr
0.069
0.073
0.238
0.005
ND
ND
Cu
0.028
0.008
0.028
ND
ND
ND
Pb
0.004
0.012
0.008
0.012
0.046
0.032
Mg
0.28
0.10
0.450
1.5
1.5
1.6
Ni
ND
ND
ND
ND
ND
ND
Zn
ND
0.009
0.073
0.162
0.169
0.185
N-NH4
190
110
280
ND
ND
ND
TS
3,100
2,900
4,500
330
110
110
TSS
43
10
120
6.8
9.0
13
TOC
33
38
30
8.4
5.5
4.0
Alk. (as CaCO-)
i
46
72
90
18
19
19
NOTES: D= Sample Duplicate
TS= Total Solids
ND= Below minimum detectable level
T0C= Total Organic Carbons
Alk.= Alkalinity
TSS= Total Suspensed Solids
-------
EdwardG. Foree, Ph.D., P.E. John S. Tapp, Ph.D., P.E., L.S.
COMMONWEALTH TECHNOLOGY, INC. ^ g 1991
Environmental and Natural Resources Consulting and Analytical Services
Lexington, Kentucky Louisville, Kentucky Grayson, Kentucky
BIOMONITORING JOB NO: 4006/EPA 4097 - Chronic Study
SAS 1109
-OO/S
L INTRODUCTION C^JT^ F*tL% , /fZ
Facility: Region I NPDES Permit No: N/A
Contact: Ms. Cindy Schreyer Phone No: (703) 519-1386
Receiving Stream: N/A Contact: Billie Rogers
Laboratory: Commonwealth Technology, Inc.
2520 Regency Road
Lexington, Kentucky 40503-2951
(606) 276-3506
II. SAMPLES (EFFLUENT AND DILUTION WATER)
1. Effluent
Sample Point: Final Effluent
Collection Dates and Times: 06/17/91/3.-30PM; 06/19/91/1:40PM; 06/21/91/1:40PM
Collection Method: 24 Hour Composite
Physical and Chemical Data: See Attached Data Sheet in Appendix A
2. Dilution Water
The water used for dilutions and control was a moderately hard reconstituted water adjusted to pH 7.8.
The physical and chemical data of the dilution water is reported in Appendix A.
III. TEST METHODS
Test Method: 'Short-Term Methods for Estimating th; Chronic Toxicity of Effluents and Receiving Waters
to Freshwater Organisms' EPA/600/4-89/001.
Deviations from methods, if any, and explanation: After warming the samples to 259C the samples with
dissolved oxygen (D.O.) readings greater than 100 percent saturation were aerated slowly until D.O. was
less than 100%.
Date and Time Test Started: 06/18/91 (3:00PM); 06/18/91 (4:30PM)
Date and Time Test Terminated: 06/26/91 (3:10PM); 06/25/91 (5:00PM)
Test Chambers: Refer to Table 1
Volume used per Chamber. Refer to Table 1
Number of Organisms per Chamber: Refer Table 1
Number of Replicates per Concentrations: Refer to Table 1
Acclimation of Organisms: Refer to Table 1
Test Temperature (mean and range): Cultured at test temperature, 25° ± 1°C
-------
TABLE 1
SUMMARY OF TEST CONDmONS FOR CHRONIC TOXICITY TEST
METHOD : EPA 600/4-89/001
Carbdaphnla dubla
Method: 1002.0
Plmaphalas promalas
Method: 1000.0
1. Test Type:
2. Temperature:
3. Light Quality:
4. Light Intensity:
5. Photoperfod:
6. Test Chamber Size:
7. Test Solution Volume:
8. Renewal of Test Concentrations:
9. Age of Test Organism:
10. No. of Test Organisms per Chamber
11. No. of Replicate Chambers per Concentration:
12. Feeding Regime:
13. Aeration:
14. Dilution Water:
15. Effluent Concentrations:
16. Test Duration:
17. Effects Measured:
18. Test Acceptability:
Static-Renewal
25* ± 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 his.
light
30 mis
IS ml/replicate
Daily
Less than 24 hours
1
10
Fed YCT (yeast suspension)
6 Selenastrum minutum
(algae) suspension daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7 days or until 60% of
fie control females have 3
broods
Survival and Reproduction
80% or greater survival
In the control concentration
Static-Renewal
25*4 1*C
Ambient laboratory
illumination (cool
white)
Approx. 100 ft-c
8 hrs. dark, 16 hrs.
light
270 ml
200 ml/replicate
Daily
1-2 days
10
3
Fed brine shrimp nauplii
in a concentrated suspension
twice daily.
Samples with D.O. greater
than 100% saturation were
aerated.
Reconstituted water (10%
Perrier water)
100%, 50%, 25%, 12.5%,
6.25%
7-days
Survival and Growth
80% or greater survival
in the control concentration
-------
IV. TEST ORGANISMS
Name: Ceriodaphnia dubia:, Pimephales promelas
Age: Less than 24 hours; 2 days
Source: Stock N; Stock F
Life Stage: Neonate; Juvenile
Mean Length and Weight (if applicable): N/A
V. QUALITY ASSURANCE
Standard Toxicant: Sodium Chloride
Date and Time of Most Recent Test:
Ceriodaphnia dubia 06/12/91 (5:30PM)
Pimephales promelas 06/22/91 (3:30PM)
Dilution Water Used: Reconstituted Water, 10% Perrier Water
Results: Ceriodaphnia dubia - LCjo (48 hrs) = 2846 mg/l NaCI
Pimephales promelas • LCM (96 hrs) = 6295 mg/L NaCI
Results are in acceptable range as determined by laboratory's testing program.
VI. RESULTS
Jtate Permit Umits: N/A; NOEL: N/A
Test Results: Toxicity was indicated with the Ceriodaphnia dubia and the fathead minnow. The NOEC
for both the Ceriodaphnia dubia and fathead minnow was 50% effluent concentration. The LOEC, ECMt
and the Chronic Values are presented in Table 2.
Data Tables and Analysis: Presented in Table 2
Indicate Statistical Methods Used: Presented in Table 2
Raw Data: Bench Sheet Data is Presented in Appendix A.
-------
TABLE 2
SUMMARY OF CHRONIC BIOMONITORING RESULTS
SAMPLES COLLECTED JUNE 17, 19, and 21, 1991
EPA NO: 4097
Survival Data Analysis
Reproduction Data Analysis
Teat
Orgenlsm
Sample
ID
Test
Concentration
(%)
1
2
% Survival by Daya
3 4 8 6
7
8
Tox.
Method
Average •
Young
Produced
Tox.
Method
Corlodaphnla
Control
...
100
100
B0
80
80
80
80
80
...
...
16
•••
...
dubla
Final Effluent
6.25
100
100
100
100
100
100
80
80
No
Inspection
18
No
Inspection
12.5
100
100
100
100
100
100
100
100
No
Inspection
16
No
Inspection
25
100
100
100
100
100
100
100
100
No
Inspection
15
No
Inspection
50
100
100
100
90
00
00
90
90
No
Inspection
22
No
Inspection
100
100
100
go
00
80
70
50
50
No
Flshefs
5
Yes
Inspection
Finding: NOEC « SOX Effluent Concentration,
LOEC ¦ 100% Effluent Concentration,
ChV » 70.7% Effluent Concentration,
EC„ ¦ 100% Effluent Concentration,
Toxicity
Survival Data Analysis
Growth Data Analysis
Test
Organism
Sample
ID
Test
Concentration
<*]
1
2
% Survival by Days
3 4 6 6
7
Tox.
Method
Average Wt.
per Larvae
(mg)
Tox.
Method
Fathead
Control
...
100
100
97
97
97
97
93
...
...
0.44
...
...
Minnow
Final Effluent
6.25
97
97
93
93
93
93
90
No
Dunnetfs
0.44
No
Dunnetfs
12.5
97
97
97
93
90
90
87
No
Dunnetfs
0.47
No
Dunnetfs
25
97
97
97
97
97
97
97
No
Dunnetfs
0.48
No
Dunnetfs
50
80
77
73
73
73
73
73
No
Dunnetfs
0.35
No
Dunnetfs
100
67
17
10
10
10
10
10
Yes
Dunnetfs
N/A
N/A
N/A
Finding: NOEC ¦ 80% Effluent Concentration,
LOEC m 100% Effluent Concentration,
ChV m 70.7% Effluent Concentration,
ECM • 64% Effluent Concentration,
Toxicity
-------
Appendix B
1991 BLACKSTONE RIVER SURVEY
DATA TABLES AND CONCENTRATION PROFILES
-------
BLACKSTONE RIVER DRY WEATHER STUDY
1991
DATA TABLES AND CONCENTRATION PROFILES
Compiled by
Raymond M. Wright, Rajat Roy Chaudhury, and Eid Alkathib
Department of Civil and Environmental Engineering
University of Rhode Island
Kingston, RI
For
U.S. Environmental Protection Agency
Region I, Boston, MA
MA Department of Environmental Protection
Grafton, MA
RI Department of Enviromental Management
Providence, RI
July 1992
-------
INTRODUCTION
This report is a compilation of the data collected for
the Blackstone River Dry Weather Study, 1991. The study was
designed to conduct a monitoring program for the Blackstone
River, it's major tributaries and the major point sources
discharging directly into the river. The data collected in
this study will be used to calibrate and validate steady
state models for dissolved oxygen (QUAL-2E) and trace metals
(PAWTOXIC).
BLACKSTONE RIVER SAMPLING PROGRAM:
The Blackstone River study consisted of three synoptic
surveys for water quality and biological studies. The study
was conducted cooperatively by the United States
Environmental Protection Agency (USEPA), the Massachusetts
Department of Environmental Protection (MADEP), Narragansett
Bay Project (NBP), Rhode Island Department of Environmental
Management (RIDEM) and the Department of Civil Engineering
at the University of Rhode Island (CVE).
Twenty one water quality stations were selected for
analysis. These included fifteen stations along the
mainstem of the river and six major tributaries. Table 1
indicates the sampling locations for the study. Two of the
largest point sources were sampled, namely, the Upper
Blackstone Mater Pollution Abatement District (UBWPAD)
facility and the Woonsocket Waste Water Treatment Facility
(WWWTF).
SAMPLE CONSTITUENTS AND FREQUENCY
The water quality sampling for each of the surveys was
divided into two parts. The first part was the sampling of
the point sources by the U.S. EPA. This phase consisted of
collecting a 24 hour composite sample for each of the 5 days
prior to the three synoptic surveys. The samples were
transported to the Environmental Engineering laboratories at
CVE.
The second phase was the river water quality sampling.
The water quality sampling crews recorded field measurements
for dissolved oxygen (DO), temperature, and pH over a 48
hour period at six hour intervals. Water quality samples
were taken during the first 24 hours of sampling. Table 2
lists the sampling frequency and the constituents analyzed
for water quality. All water samples were transported to
the Woonsocket WWTF for processing by CVE personnel.
DATA ORGANIZATION
The presentation of the data in this report occurs in
two forms. The first is tabular, listing the concentration
-------
of the constituent(s) for each sampling run. The second
graphical, indicating the spatial variation of the
constituent(s) concentration. Plots illustrating the
temporal variation of DO for the twenty one stations are
also presented.
-------
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BLACKSTONE-RIVER
STATION 1
i r
JULY 10-11, 1991
DO CRITERIA
SATURATION DO
O OBSERVED DO
_L
8 12 16 20 24 28 32 36 40 44 4S
i r
-
DO
CRITERIA
AUGUST 14-15,
1991
SATURATION DO
i i i i
i
i i
O
i
OBSERVED DO
i i *
0 4-8 1216
20
24 28
32
36 40 44 4
ir
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
4 8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
nr\
p
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>
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(/)
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52
a
2
0
BLACKSTONE RIVER
STATION 2
n r
12 -
0
s
6 i-
4 h DO CRITERIA
JULY 10-11, 1991 SATURATION DO
O OBSERVED DO
16 20 24 28 32 36 40 44 ^3
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO '
C OBSERVED DO 1
i i ' ' i ' - i i i i i
12 16 2C 24- 28 32 35 40
4S
DO CRITERIA
OCTOBER 2-3, 1991
iii'i
SATURATION DO
O OBSERVED DO
—i i i . i
3 12 16 20 24 28 32 36 40 44 43
TIME (HOURS)
-------
BLACKSTOXE RIVER
STATION 3
: 4
12 i-
10
a
6 •-
4
2
0
1 4
12
10
s
6
4
2
0
0
1 4
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2
in
Q
0
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JULY 10-11, 1991
DO CRITERIA
O
SATURATION DO
OBSERVED DO
8 12 16 20 24 23 32 36 40 44 48
T
T
DO CRITERIA
AUGUST 14-15, 1991
O
SATURATION DO
OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
-O
DO CRITERIA
OCTOBER 2-3, 1991
O
SATURATION DO
OBSERVED DO
4 8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
1 4.
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BLACKSTONE RIVER
STATION 4
i r
JULY 10-Li, 1991
DO CRITERIA
SATURATION DO
O OBSERVED DO
8
12 16 20 24 28 32 35 40 44
_
i i i i
i i ¦ - r " 1 i
' • i
-
J0L
—
— -i
-O— ^ —0
_
DO CRITERIA
AUGUST 14-15,
1991
SATURATION DO
i i i i
O
' i . i i
OBSERVED DO
i i.i
8 12 16 20 24 28 32 36 40 44 48
i i i i i i i i i r
-O O
DO CRITERIA
OCTOBER 2-3, 1991
I'll! 1
o
SATURATION DO
O OBSERVED DO
1 I. J !
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 5
_l
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JULY 10-11, 1991
DO CRITERIA
1
SATURATION DO 1
O OBSERVED DO 1
a
12 16 20 24 28 32 36 40 44 48
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
O OBSERVED DO
i ¦ i
8 12 16 20 24 28 32 36 40 44 48
i r
i r
O O
-o-
-o
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
4 8 12 15 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
4
<•}
0
8
6
4
2
0
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2 ¦
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8 ¦
6 ¦
4 ¦
2 ¦
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0
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2 ¦
0 ¦
8 ¦
6 ¦
4 ¦¦
2 ¦
0 -
BLACKSTONE RIVER
STATION 6
i r
DO CRITERIA
JULY 10-11, 1991 SATURATION DO
O OBSERVED DO
J _L
J L
4 8 12 16 20 24 28 32 36 40 44 48
i i i i i i i i i i ; i
DO CRITERIA
AUGUST 14-15. 1991 SATURATION DO
O OBSERVED DO ~
_j i i i i i i i i i i 1
4 8 12 16 20 24 28 32 36 40 44 48
"i 1 r
i r
DO CRITERIA
OCTOBER 2-3, 1991
_l_
SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION1 7
,11!
1
1
1
! :
1 ¦ ;
i
\
o cr
TJ V O _
- DO CRITERIA
SATURATION D0-
JULY 10-11,
i , i
1991
; 4 i i
V
i
OBSERVED DO
DO BELOW DAM ~
i i
0 4 8 12
—i i r ¦
16 20 24 28
i 'i i
32
—¦!"
36 40 44 4
III]
o
8
6 -
DO CRITERIA
AUGUST 14-15, 1991
' i i
SATURATION DO-j
OBSERVED DO !
V DO BELOW DAM 1
8 12 16 20 24 28 32 36 40 44 48
i 1 1 r
9
1 1 1 r
"9"
-¦w'
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
OBSERVED DO
V DO BELOW DAM
i i i i
4 8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
—
1 4
CP
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BLACKSTONE RIVER
STATION 8
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DO CRITERIA
JULY 10-11,
1991
SATURATION DO
i i i
O
1 1 1 ! 1
OBSERVED DO
! ! 1
12 16 20 24 28 32 '36 4Q
J.Q
1 1 ! 1 I'" "'" t r
i. i i
-
0 _
DO CRITERIA
-
AUGUST 14-15, 1991
SATURATION DO
O
i i i i i i i i
OBSERVED DO
i i i
8
12 16 20 24 28 32 36 40 44 48
i 1 1 r
i r
i r
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
1 4
\
V
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£
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STATION 9
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- DO CRITERIA
JULY 10-11,
1991
SATURATION DO-
OBSERVED DO J
V DO BELOW DAM ~j
12
6 20 24 28 32 36 40 ^4 AS
i i i i
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' 1 1 1 ' 1 1 " ! "
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- °D0 CRITERIA
AUGUST 14-15,
! 1 ! 1
1991
i I
SATURATION DO-
OBSERVED DO
V DO BELOW DAM ~
_ i i . J t. . i
8 12 16 20 24 28 32 36 . 40 44 48
DO CRITERIA
OCTOBER 2-3, 1991
i : ! I
SATURATION DO
OBSERVED DO
V DO BELOW DAM
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
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STATION li
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DO CRITERIA
JULY 10-11, 1991 SATURATION DO
O OBSERVED DO
8 12 16 20 24 23 32 36 40 44 42
DO CRITERIA
AUGUST 14-L5, 1991
SATURATION DO
O OBSERVED DO 1
8 12 15 20 24 28 32 36 40 44 48
-O
-O J
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
0 4 8 12 16 20 24 28 32 36 40 44 48
T'ME (HOURS)
-------
BLACKSTONE RIVER
STATION 12
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JULY 10-11, 1991 SATURATION DO
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DO CRITERIA
-
AUGUST 14-15,
1991
SATURATION DO
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8 12 16 20 24 28 32 36 40 44 48
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DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACK3T0NE RIVER
STATION 13
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0
JULY 10-11, 1991
DO CRITERIA
SATURATION DO I
O OBSERVED DO I
12 15 20 24 28 32 36 40 44 48
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DC
O OBSERVED DO 'j
i . I i . i i i i i i i i I
4 8 12 16 20 24 28 32 36 40 44 48
T
T
o-
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DO CRITERIA
OCTOBER 2-3, 1991
i i I i l I i—
SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 14
1 2
10
o
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>
X
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5
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4
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DO CRITERIA
JULY 10-11, 1991 SATURATION DO
O OBSERVED DO
i . i i i i ' i i i i
8 12 16 20 24- 28 32' 36 40 44
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
O OBSERVED DO
i i i i ' i i i i i '
8 1'2. 16 20 24 28 32 36 40 44 48
1 1 1 r
O——O-
DO CRITERIA
OCTOBER 2-3, 1991
_L
SATURATION DO
O OBSERVED DO
i i i i
8 12 16 20 24 28 32 36 40 44 4g
TIME (HOURS)
-------
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2
0
BLACKSTONE RIVER
STATION 15
I I
DO CRITERIA
JULY 10-11, 1991
SATURATION DO ;
O OBSERVED DO 1
8 12 16 20 24 28 32 36 40 44 48
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
a
-O
-o
-o
DO CRITERIA
OCTOBER 2-3, 1991
-0
SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTON'E RIVER
STATION 16
10
s
r\
4
2
0
1 4
12
10
8
5
4
2
0
JULY 10-11, 1991
DO CRITERIA
SATURATION DO
O OBSERVED DO
-J
8 12 16 20 24 28 32 36 40 44 4?
i i 1 1 1 1 r
J
-O
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 48
i r
n r
12
10
8
6
4 -
2 -
)
O -o o-
D0 CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
0 4 8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 17
DO CRITERIA
JULY 10-11. 1991
SATURATION DO
O OBSERVED DO '
0
8 12 16 20 24 28 32 36 40 44 43
DO CRITERIA
AUGUST 14-15, 1991
J
SATURATION DO
O OBSERVED DO
0 4 8 12 16 20 24 28 32 36 40 44 48
DO CRITERIA
OCTOBER 2-3, 1991
_L
SATURATION DO
O OBSERVED DO '
0 4.5 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 18
z
LJ
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>
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Q
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>
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6
4
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0
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o-—o
DO CRITERIA
JULY 10-LI, 1991 SATURATION DO
O OBSERVED DO
8 12 16 20 24 28 32 36 40 44 43
n i i i r
-O o O -o-
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
0 OBSERVED DO
, i 1 i i i i i I i i i
I- 8 12 16 20 24 28 32 36 40 44 48
x
o 6 -
o
£ 4 - DO CRITERIA
o 2 - OCTOBER 2-3, 1991 SATURATION DO _
$ O OBSERVED DO
Q 0 1 1 1 1 ' J 1 1 I ' I
0 4 8 12 16 20 24 28 32 36 40 44 43
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 19
£
z
LU
o
>-
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Ld
>
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10
8
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4
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2
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JULY 10-11, 1991
DO CRITERIA
SATURATION DO
OBSERVED DO
DO CRITERIA
AUGUST 14-15, 1991
O
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' 'v ^
SATURATION DO
OBSERVED DO
_i_
x
8 12 15 20 24 28 32 36 40 44 48
x
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> 4 - DO CRITERIA
o 2 - OCTOBER 2-3, 1991 SATURATION DO _
$ O OBSERVED DO
o o 1 i i i i i i i i i i
0 4 8 12 16 20 24 28 32 35 40 44 48
TIME (HOURS)
-------
BLACKSTONE RIVER
STATION 20
3 L
6
4
2
-O.
&
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DO CRITERIA
JULY 10-11 1991 SATURATION DO
O OBSERVED DO "
j >
C 4 8 12 16 20 24 23 32 36 4Q ^4 43
DO CRITERIA
AUGUST 14-15, 1991
SATURATION DO j
O OBSERVED DO
J L
8 12 .16 20 24 23 32 36 40 44 43
-i r
0
a
6
4 -
2
1 !
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
1
0
J L
0
8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
BLACK5T0NE RIVER
STATION 21
—
1 4
rp
r-
! ^
w
W
10
z
" 1 1
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o
8
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X
o
6
Q
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2
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1 4
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v
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CP
1 2
£
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8
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m
2
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Q
0
14
12
10
8
6
4
2
0
JULY 10-11, 1991
DO CRITERIA
SATURATION DO
O OBSERVED DO
12 16 20 24 28
35 40 44 45
DO CRITERIA
AUGUST 14-15, 1991 SATURATION DO
O OBSERVED DO
a 12 16 20 24 28 32 36 40 44 48
1 r
1 r
o—-o
-o-
¦o
-o
DO CRITERIA
OCTOBER 2-3, 1991
SATURATION DO
O OBSERVED DO
4 8 12 16 20 24 28 32 36 40 44 48
TIME (HOURS)
-------
1991 Blackstone River Survey #1
Total (TTM) and Dissolved (DTM) Cadmiun
in jig /L
Station
Run
#1
Run
n
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
0.33
WD
. 0.32
0.05
0.40
ND
0.33
0.08
BLKQ2
3 .09
2.49
4.34
3 .03
3.52
3.45
3.54
3.30
BLK03
2.57
2.14
2.54
1.38
2.37
2.52
2.39
1.54
BLX04
1.95
1.33
2.17
1.59
2.34
1.04
1.64
1.07
BLKOS
0.10
ND
0.05
ND
0.09
ND
0.42
0.34
BLK06
1.19
0.47
0.93
0.54
1.21
0.91
1.06
0.77
BLK07
0.77
0.12
0.37
0.25
0.76
0.64
1.09
0.92
3LK08
1.30
0.11
1.05
0.16
1.10
0.23
1.74
0.17
BLK09
0.05
ND
0.16
ND
0.20
ND
0.12
ND
BLK10
0.06
ND
ND
ND
0.33
ND
2.34
ND
BLX11
0.77
0.23
1.20
0.23
1.32
0.23
1.34
0.43
BLX12
0.64
0.36
0.62
0.22
0.39
0.17
0.45
0.08
BLK13
0.92
0.12
0.59
0.13
0.63
0.15
0.56
0.19
BLK14
0.18
ND
0.07
ND
ND
ND
0.11
ND
BLX15
O.OS
ND
0,06
ND
0.13
0.09
0.09
ND
BLK16
0.54
0.25
0.09
ND
0.33
0.23
0.29
0.13
BLK17
0.57
0.33
0.40
0.05
0.59
0.26
0.50
0.10
3LK18
0.53
0.16
0.55
0.22
0.55
0.45
0.57
0.23
BLX19
0.35
0.12
0.38
0.13
0.42
0.22
0.47
0.13
BLK20
0.35
0.15
0.24
0.06
0.30
0.21
0.36
0.12
BLK21
0.23
ND
0.29
ND
0.40
0.13
0.33
0.17
ND = Below the detection limit of 0.05 nq/L,
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP-
UBWPAO
ZL
< ^
o
A
WEST
RIVER
NORTH- MUMEORD
BRIDGE R,VER ~
WWTP
WOON.
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey I, July 10 - 1 1,1991
Point Sources and River Inputs
Water Quality Stations, 1991
4
i
-J l i L_
50
45
I
A
i
i l
40
A
1
_j. _ I 1. _i I. _l _ 1. -J I.
35
30
12 13
£
T
A
25
17
20
18
A A
15
19 20
A
21
A
10
A
. i
5
RIVER MILES
-------
1991 BlacJcstone River Survey $2
Total (TTM) and Oissolved (DTM) Cadaium
in jug/L
Station
Run
#1
Run
n
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLKQ1
0.28
0.22
0.31
0.23
0.38
0.20
0.33
0.34
BLKQ2
4.15
3.27
4.40
4.11
3.91
3.87
4.16
3.32
BLK03
3.50
2.93
3.61
2.94
3.42
3.25
3.52
3.32
BLK04
2.83
2.10
2.75
2.68
3.46
2.78
2.54
2.15
BLX05
0.15
0.16
0.13
0.12
0.09
0.05
0.11
ND
BLK06
1.73
1.56
1.50
1.43
1.32
1.64
2.38
2.09
BLX07
1.02
0.72
0.78
0.70
0.95
0.74
1.15
1.03
BLK08
2.08
1.29
1.43
1.08
1.70
1.30
2.03
1.37
BLX09
HD:
ND
0.35
0.31
0.09
0.07
0.13
ND
BLK10
0.08
0.08
0.17
0.18
0.38
0.17
0.07
0.10
BLK11
0.95
0.42
0.92
0.74
1.18
0.81
0.93
0.67
BLX12
0.78
0.50
0.81
0.82
3.53
1.05
0.86
0.53
BLK13
0.67
0.54
0.48
0.40
1.14
0.99
0.53
0.25
BLX14
0.23
0.21
0.11
0.10
0.34
0.25
0.37
ND
BLX15
0.16
0.08
0.12
ND
0.08
HD
0.35
ND
BLK16
0.16
0.16
0.06
0.05
0.23
0.12
0.14
0.17
BLK17
0.50
0.30
0 .*33
0.23
0.51
0.21
0.54
0.32
BLK18
0.89
0.76
1.08
0.81
0.82
0.73
1.24
0.70
BLX19
0.57
0.57
0.73
0.57
0.93
0.92
0.15
0.21
BLX20
0.56
0.45
0.49
0.38
0.49
0.30
0.26
0.08
BLX21
0.45
0.42
0.38
0.33
0.47
0.24
0.40
0.43
ND » Below the detection limit of 0.05 nq/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP-
UBWPAO
WEST
RIVER
NORTH- MUMFORQ
BRIDGE RtvER ~
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
1 1
T
A
x
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
12
13
1 7
18
19
20
21
A
—I I—
50
A
T
t
45
I
A
1
40
A
35
T
A
1
_i 1 1
30
A
A
25
T
A
1
T
A
A
..iLi I
20
T
f
A
10
A
. A
b
RIVER MILES
-------
1991 Blacks-cone River Survey #3
Total (TTM) and Dissolved (DTM) Cadmium
in ^g/L
Station
Run
#1
Run
#2
Run
^3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
0.42
0.28
0.14
0.10
0.23
0.15
0.35
0.11
BLK02
0.52
0.52
1.35
1.17
2.35
2.30
3.14
2.60
BLK03
3.82
3.62
1.21
0.87
2.68
1.64
2.72
2.10
BLK04
1.3S
1.01
1.13
0.62
2.11
1.47
2.00
1.37
BLX05
0.09
ND
ND
ND
ND
ND
ND
ND
BLX06
2.79
0.31
1.06
0.58
1.48
1.03
1.43
0.90
BLX07
1.08
1.01
1.18
0.79
1.14
0.80
1.28
0.77
BLK08
1.64
1.04
1.44
0.92
1.30
0.90
1.42
0.96
BLX09
0.12
ND
0.09
ND
0.08
ND
0.13
ND
BLK10
0.20
0.13
0.06
ND
0.07
0.05
ND
ND
BLX11
1.38
0.96
1.39
0.61
1.22
0.71
1.16
0.57
BLX12
1.18
0.68
1.29
0.38
1.16
0.89
1.05
0.57
BLK13
1.28
0.63
1.02
0.55
1.22
0.67
0.96
0.45
BLX14
0.09
0.09
0.06
0.12
0.06
ND
0.10
ND
BLK15
0.05
ND
ND
ND
0.12
ND
ND
ND
BLK16
0.05
ND
0.15
0.22
0.11
ND
ND
ND
BLX17
0.32
0.47
0.53
0.66
0.71
0.41
0.68
0.41
BLX1S
0.90
0.49
0.49
0.26
0.68
0.39
0.77
0.15
BLK19
0.56
0.49
0.42
0.19
0.56
0.30
0.64
0.36
BLX20
0.56
0.39
0.37
0.23
0.43
0.35
0. 65
0.43
BLK21
0.55
0.07
0.36
0.26
0.43
0.27
0.60
0.41
ND ' 3elow the detection limit of 0.05 nq/L.
-------
Blackstone River
GRAETON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
3
7
WEST
RIVER
NORTH— MUMFORD
BRIDGE R,VER ~
WWTP
WOON
WWTP
BRANCH
RIVER
O
cr>
3
5 3
o
< n
~ £
0
A
3 4
1 1
A
A
A
A
A
A
50
45
40
35
30
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A A
I . I- * _1 i I 1 « 1 1 1 i_ i _ * _l i i i i I i i i i I i i i
A A
i
12 13 17 18 19 20 21
A A
_i 1 1 L_
J 1 1. i -l I 4 i I J 1 t_. .1 i. t...
25
20
15
10
Li
RIVER MILES
-------
1991 Blacks tone River Survey jfi
Total (TTM) and Dissolved (OTM] Chromium
in pg/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
4.5
0.4
4.3
2.0
0.3
1.0
5.0
1.4
3LK02
4.7
2.1
5.1
3.5
3.3
2.4
6.3
3.5
BLK03
3.4
3.0
3.7
2.0
1.8
1.6
4.3
2.7
BLK04
3.8
2.1
2.4
1.5
3.2
2.1
4.3
2.7
BLX05
ND
ND
ND
ND
0.2
ND
ND
0.9
BLX06
5.3
1.6
2.6
0.9
1.4
1.2
4.5
1.9
BLK07
6.4
1.1
3.4
0.4
3.9
1.1
5.7
2.9
BLKOa
24. 5
1.3
6.9
0.3
2.0
2.1
26.3
1.4
BLK09
0.9
0.6
0.5
ND
0.9
0.3
1.4
0.3
BLX10
0.4
ND
2.8
ND
0.7
ND
0.9
ND
BLK11
6.1
1.6
7.4
0.9
3.6
0.8
10.2
1.2
BLK12
2.8
2.0
2.2
0.6
1.7
0.9
2.5
0.7
BLX13
2.2
0.9
4.4
0.6
2.5
0.6
2.7
0.8
BLX14
ND
ND
0.9
0.5
ND
ND
0.8
0.4
BLK15
0.5
0.2
ND
ND
ND
ND
0.3
ND
BLX16
0.6
ND
0.2
0.3
2.1
0.2
0.7
ND
BLK17
1.5
0.6
1.0
0.3
1.2
0.7
1.6
0.4
BLKia
1.9
0.4
2.4
1.0
1.7
1.1
1.6
1.9
BLX19
0.7
0.3
1.5
0.9
1.2
0.5
1.3
1.5
BLK20
1.1
0.3
1.0
0.3
1.0
0.6
1.1
0.4
BLK21
0.9
0.4
1.1
0.3
1.5
1.3
1.4
0.7
ND » Below the detection limit of 0.2 ugiL.
-------
Blackstone River
GRAFTON
WWfP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n
WEST
RIVER
NORTH— MUMFORD
BRIDGE R,VER
WWTP
WOON,
WWTP
BRANCH
RIVER
r i r
3 4
A
1 t
12 13
18
19 20
21
LEGEND:
: Survey I, July 10-11, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
I
A
1
50
T
i*
45
1
40
T
t
35
I
A
1
30
A
, i
25
T
A
1
A
20
A
15
A
A
. J
10
A
I . i
5
RIVER MILES
-------
1991 Blackstone River Survey i2
Total (TTM) and Dissolved (DTM) Chromium
in nq/L
Station
Run
n
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLX01
4.9
3.4
9.6
5.9
24.5
11.2
5.9
4.3
BLX02
S. 7
4.3
4.9
3.3
6.6
7.3
8.3
6.9
BLK03
4.9
3.3
4.5
3.0
4.2
2.3
6.0
4.3
BLX04
4.3
2.1
4.1
2.9
3.9
2.9
3.6
2.9
BLK05
0.7
ND
1.6
0.4
1.0
0.3
0.4
ND
BLK06
4.2
1.7
3.1
1.9
4.2
2.2
4.2
2.1
BLK07
5.3
1.4
4.6
2.2
3.1
1.7
3.2
1.4
BLX08
14.9
4.1
3.3
1.3
11.4
2.4
12.9
2.6
BLK09
1.4
0.6
1.7
0.7
1.6
0.4
1.2
0.7
BLK10
0.7
ND
0.5
ND
2.0
1.0
0.3
1.1
BLK11
6.0
1.1
4.3
2.3
6.7
1.7
5.1
1.4
BLK12
3.2
1.1
3.3
1.3
3.1
1.0
3.1
1.4
BLK13
3.2
1.5
2.4
1.2
3.1
1.2
2.4
0.7
BLX14
1.1
0.5
1.0
1.1
0.3
0.3
1.2
0.6
BLK15
0.5
0.2
0.5
ND
0.3
ND
0.7
0.3
BLK16
0.6
0.2
0.3
ND
0.4
ND
0.9
0.5
BLK17
1.9
0.8
1.5
0.6
1.9
0.3
2.3
0.3
BLK13
3.2
1.4
2.0
1.3
1.3
1.0
10.5
6.1
BLK19
1.3
0.6
2.5
1.1
2.2
1.0
1.7
1.1
BLK20
1.3
0.9
2.3
0.7
1.3
0.7
2.8
0.9
BLK21
1.7
0.9
1.7
0.7
0.7
0.6
1.7
0.8
ND = Below the detection limit of 0.2 /ig/L.
-------
Blackstone River
GRAFTON
WWTP
OUIN.
MILBURY RIVER
WWTP -
UBWPAD
-II
WEST
RIVER
NORTH- MUWFORD
BRIDGE R|V£R ""
WWTP
WOON
WWIP
BRANCH
RIVER
A
50
T
A
1
I I
3 4
11
1 :
-J 1 1 U.
LEGEND:
Survey II, August 14—15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
i
£
A A
T
Z-i
1
T
A
_1__J l L-
-1 1 L-
1 2
13
17
18
19
I
20
21
A
A
A
45
40
35
30
25
?0
A A
0
&
RIVER MILES
-------
1991 Blackstone River Survey #3
Total (TTM) and Dissolved (DIM) Chromium
in jig/L
Station
Run
*1
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
2.8
1.6
2.8
1.1
4.2
2.1
5.7
1.6
BLK02
3.0
1.6
2.3
1.0
2.9
1.5
4.6
2.2
BLK03
3.0
1.7
2.5
0.3
3.2
1.1
4.2
2.0
BLK04
2.9
1.4
2.5
0.6
2.0
0.7
3.0
1.3
BLK05
0.2
ND
0.2
ND
0.2
ND
ND
ND
BLX06
2.4
0.4
2.5
1.0
2.1
0.6
2.3
0.5
BLX07
3.5
2.1
3.1
0.9
2.4
0.7
3.5
0.9
BLK08
5.2
1.3
3.7
1.0
3.2
0.8
4.1
1.4
BLK09
0.9
0.4
1.1
0.4
1.0
0.3
0.9
0.4
3LX10
0.5
ND
0.4
0.6
0.3
ND
0.3
ND
BLK11
5.2
3.4
5.4
0.9
4.6
1.1
4.4
0.6
BLK12
4.6
1.6
5.9
0.8
4.4
1.8
3.9
0.2
BLX13
4.7
1.0
3.8
0.7
4.2
0.6
13.0
1.5
BLK14
0.3
0.3
0.8
0.4
0.3
ND
0.5
ND
BLK15
0.4
ND
0.3
ND
ND
ND
0.2
ND
BLK16
0.3
ND
0.4
ND
ND
ND
ND
ND
BLX17
3.4
1.5
3.2
0.8
2.7
0.8
3.4
0.7
8LK18
3.6
0.9
3.3
0.8
2.5
0.5
3.0
0.7
BLX19
3.5
o.a
2.3
0.7
2.4
0.7
2.2
0.6
BLK20
2.9
0.6
2.3
0.7
2.9
1.3
2.1
0.5
BLK21
3.7
1.2
1.2
1.1
2.5
1.1
2.2
0.6
ND » Below the detection limit of 0.2 M9/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN
MJLBURY RIVER
WWTP
UBWPAD
30
25
I I I I
WEST
RIVER —
NORTH- MUMFORO
BRIDGE RIVER
WWTP
WOON
WWTP
BRANCH
WVER
CP
20
15
10
0
T
f
3 4
11
1 :
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
&
T
£
I 1 1 1 1 L-
*****
50
45
40
35
30
*. *
12 13
A
17
A
18
A
_V i ) 1_
25
20
15
19
20
A A
10
21
T
A
i
_i-L
RIVER MILES
-------
1991 Blacks-tone River Survey #1
Total (TTM) and Dissolved (DIM) Copper
in Mg/L
Station
Run
#1
Run
*2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
15.0
6.1
6.7
1.9
6.9
3.0
8.7
1.4
BLX02
48.8
37.1
38.7
27.2
29.8
26.4
28.8
26.9
BLK03
45.3
22.7
29.0
22.1
25.6
25.2
26.3
21.4
BLK04
28.3
19.9
25.9
20.4
26.9
19.9
27.6
19.5
BLK05
1.3
ND
1.6
ND
1.2
ND
5.4
3.9
BLK06
29.6
15.1
21.7
14.5
17.2
16.9
20.2
14.4
BLK07
22.8
7.9
22.8
8.6
19.9
20.0
21.0
15.1
BLK08
45.5
7.5
24.1
8.3
14.3
10.2
53.8
7.0
BLK09
11.0
ND
9.2
ND
6.6
ND
2.5
ND
BLK10
41.0
1.3
1.4
ND
10.7
ND
23.4
ND
BLK11
15.7
5.1
23.0
6.4
25.5
8.5
25.7
7.6
BLK12
11.9
7.3
11.6
5.2
8.8
8.1
11.4
4.6
BLK13
a. 8
5.2
9.9
4.1
14.1
7.1
8.5
2.8
BLK14
7.4
1.2
7.1
2.9
1.8
0.6
6.8
ND
BLK15
4.8
3.1
3.7
ND
4.3
2.2
2.4
ND
BLK16
24.9
14.7
2.9
2.6
37.4
4.6
2.0
ND
BLX17
9.0
4.5
10.4
2.9
13.0
7.2
7.3
1.8
BLX13
€.5
2.3
10.9
3.7
10.7
10.3
7.6
9.4
BLK19
7.5
3.8
6.8
3.2
9.1
6.6
7.1
5.4
BLK20
6.9
3.7
8.7
3.7
7.7
5.2
5.9
2.9
BLK21
6.7
3.4
8.6
3.7
8.5
5.1
6.7
3.8
ND ¦ Below the detection limit of 0.2 M9/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~
WWTP
WOON
WWTP
BRANCH
RIVER
1 2-346
8 1 1
12
13
1 7
18
19 20
21
A
A
A
LEGEND:
Survey I, July 10-11, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
4
I
A
i
T
'f
A
T
A
i
4
T
A
i
A
T
A
X
A
i. i- i i
50
I 1 I l_
45
40
35
-j-.. I
30
-J I 1 L~
25
-4. 1 1 L.
20
J a -
15
10
0
RIVER MILES
-------
1991 Slaclcstone River Survey ,#2
Total (TTM) and Dissolved (DTM) Copper
in pq/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
9.9
6.3
7.6
5.3
11.5
6.0
10.1
9.0
BLK02
26.3
23.2
23.9
20.5
27.7
23.6
22. b
20.3
BLKO 3
28.3
22.4
22.4
13.0
30.8
26.6
22.7
19.1
BLKO 4
29.0
24.9
21.2
18.2
26.3
22.1
22.3
19.2
BLK05
2.1
MO
3.4
0.3
3.2
2.5
1.0
ND
BLKO 6
25.5
18.5
22.3
20.6
26.0
20.0
24.5
18.0
BLK07
20.1
10.8
13.3
10.2
21.9
12.5
16.4
12.4
BLKO 8
26.8
15.0
16.0
10.3
29.9
17.7
25.2
16.4
BLK09
1.3
ND
2.9
0.5
2.1
0.9
1.3
ND
BLK10
1.0
ND
1.5
0.3
4.8
3.5
3.4
2.7
BLK11
16.3
6.0
14.3
7.2
20.4
11.6
14.2
6.4
BLK12
11.8
7.2
12.2
11.3
14.8
3.8
10.8
5.8
BLK13
10.8
6.0
8.6
5.1
13.5
8.1
8.1
4.1
3LK14
3.4
1.5
2.5
2.3
2.1
1.8
5.2
1.5
BLK15
4.6
1.6
3.3
ND
ND
ND
6.6
ND
BLK16
3-l
0.5
1.2
1.7
3.0
3.1
2.6
2.3
BLK17
7.3
5.4
6.0
3.2
7.4
4.3
8.3
3.9
BLK13
10.4
9.5
10.7
6.7
7.8
5.5
13.1
6.0
BLX19
9.1
6.5
8.7
6.9
9.4
6.9
12.3
5.7
BLK20
8.8
7.8
9.3
5.5
10.6
5.5
9.0
5.6
BLK21
7.7
5.3
11.7
6.7
12.4
6.4
8.1
5.3
ND « Below the detection limit of 0.2 iiq/L.
-------
Blackstone River
GRAFTON
WWTP
70
60
50
40
30
20
10
QUIN.
MILBURY RIVER
WWTP
UBWPAD
WEST
RIVER
NORTH- MUMFORD
BRIDGE R1VER
WWTP
WOON
WWTP
BRANCH
RIVER
0
-A
50
3 4
r T T
t ff
45
A
40
T
A
1
35
11
A
T
A
1
12
13
17
18
19
20
21
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
T
A
T
A
1
A
30
25
20
T
A
l
1 5
T
A
±
A
1
i.
i.... i~ —
10
RIVER MILES
-------
1991 Blackstone River Survey #3
Total (TTM) and Dissolved (DTM) Copper
in nq/l>
Station
Run
#1
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
11.2
5.4
4.3
2.7
3.9
3.6
11.6
4.8
BLK02
14.1
12.3
12.5
8.3
17.2
15.4
17.5
14.5
BLK03
16.3
12.5
9.1
6.8
18.3
13.6
19.5
14.6
BLX04
14.5
12.3
11.0
5.6
16.3
13.5
16.9
15.8
BLK05
1.4
0.3
1.2
1.2
1.3
1.2
2.9
2.5
BLK06
16.9
3.2
8.4
8.0
15.2
10.3
14.6
10.0
BLK07
17.3
13.1
13.5
7.6
12.4
3.6
15.7
12.1
BLX03
19.9
11.0
16.4
11.3
14.4
10.2
17.8
10.2
BLKQ9
5.2
2.0
2.1
0.8
2.3
1.0
5.3
3.1
BLX10
1.6
1.5
1.7
1.0
2.3
1.0
9.3
2.9
BLK11
16.3
10.6
21.0
6.1
14.3
8.0
13.9
6.6
BLK12
13.0
7.0
15.5
5.0
14.0
10.9
13.1
6.8
BLK13
12.9
7.2
12.3
5.9
20.3
6.9
12.5
5.9
BLX14
4.2
3.6
3.9
3.8
2.1
2.2
4.1
1.8
BLK15
2.7
2.0
2.8
1.3
6.8
1.1
1.3
1.4
BLK16
2.9
2.5
3.3
2.1
3.1
3.2
1.6
0.3
BLX17
9.3
5.1
19.3
15.5
9.2
5.6
9.2
6.1
BLX13
13.3
6.0
3.3
3.2
7.4
5.6
10.9
5.9
BLX19
7.2
4.4
7.4
5.3
10.0
5.8
3.9
6.0
BLX20
4.8
3.4
7.5
4.2
8.1
11.3
3.9
7.6
BLX21
6.4
6.1
6.3
5.3
9.9
5.6
8.5
5.3
ND a Below the detection limit of 0.2 nq/L.
-------
Blackstone River
GRAFTON
WWTP
70
60
50
CC 40
LJ
Q_
CL
o 30
O
20
10
QUIN.
MILBURY RIVER
WWTP
UBWPAD
3
0
T
A
1
50
T
A
1
WEST
RIVER
NORTH-
BRIDGE
WWTP
MUMF0RD
RIVER —I
I
t
T
A
1
45
1 2^46
i
i
40
W00N.
WWTP
BRANCH
RIVER
T
A
1
£
1 1
T
A
1
12 13
17
18
19 20
21
1 :
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
I
4 f
_i_ 1_ t.—1__
35
30
25
20
T
A
1
15
A
T
/ A
A
10
_j i___l - _i .
5
0
RIVLR Mil I- 5
-------
1991 Blackstone River Survey, #1
Total (TTM) and Dissolved (DTM) Nickel
in pq/L
Station
Run
#1
Run
*2
Run
#3
Run
M
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
4.9
2.8
4.6
3.1
4.1
3.4
7.0
4 . 6
BLK02
32.9
27.3
30.1
25.6
23.2
23.5
27. 1
24.4
BL2C03
27.1
20.9
22.8
20.6
22.5
21.3
20.6
17.3
BLK04
11.2
7.6
19.2
13.2
18.3
16.6
13.3
15.8
BLX05
ND
ND
1.5
ND
ND
ND
1.0
1.7
BLKQ6
9.7
6.9
11.3
7.3
11.0
10.8
11.6
10.6
BLX07
7.6
5.4
9.3
7.5
8.1
8.0
10.1
7.3
BLX08
11.3
6.5
9.3
6.7
7.5
6.1
13.3
5.4
BLK09
ND
ND
1.9
ND
1.3
ND
1.1
ND
BLK10
ND
ND
1.5
ND
7.6
ND
3.5
ND
BLK11
4.7
4.9
3.5
5.4
7.8
5.1
6.8
6.1
BLX12
3.9
2.7
7.9
5.5
6.2
5.8
6.1
4.1
BLK13
3.5
3.1
7.0
4.9
7.0
5.1
7.2
4.5
BLK14
ND
ND
1.3
1.3
1.3
1.3
1.9
ND
BLK15
ND
ND
0.9
ND
1.7
2.0
1.1
ND
BLK16
4.7
1.7
1.5
1.3
9.2
2.7
0.3
ND
BLK17
6.S
4.3
8.7
3.0
6.5
4.5
6.5
3.9
BLK13
2.9
0.5
4.9
3.7
5.9
6.3
6.0
5.5
BLK19
1.6
2.1
5.1
4.4
5.6
5.0
5.3
5.2
BLK2Q
3.1
1.9
5.1
3.9
4.5
4.5
5.1
3.9
BLX21
3.0
1.9
4.3
2.2
4.9
3.4
5.8
4.2
ND » Below the detection limit of 0.2 M9/L.
-------
Blackstone River
GRAFTON
WWTP
60
50
QUIN.
MILBURY RIVER
WWTP
UBWPAD
3
WEST
RIVER
NORTH— MUMFORD
BRIDGE R,VER ~
WWTP
WOON
WWTP
BRANCH
RIVER
CJt 40
o
<
I—
o
30
20
10
0
A
I
f
T
f
I
1 2 3 4 6
A
A
T
t
11
T
£
-t I t - I
_J 1 1 i_
_1 1 I. -1—
> i- I
T
~
i
12 13
17
18
19 20
21
LEGEND:
Survey I, July 10-11, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
. > i .1 L_
50
45
40
35
30
25
20
A
15
A
1
A
10
A
x
5
. i.
0
RIVER MIL ES
-------
1991 Blackstone River Survey #2
Total (TTM) and Dissolved (DTM) Nickel
in jig/L
Station
Run
#1
Run
#2
Run
*3
Run
.#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
3LK01
9.6
8.3
6.6
5.7
6.2
5.8
8.5
8.4
BLK02
41.0
38.5
34.5
32.8
40.1
38.3
51.8
50.3
BLX03
41.4
34.5
31.3
28.5
34.2
33.1
41.1
35.3
BLK04
40.1
30.7
30.7
28.7
32.6
30.3
31.7
30.2
3LX05
2.0
1.2
1.2
0.2
1.9
0.9
0.7
0.4
BLK06
26.6
24.3
20.3
20.9
26.1
25.0
25.8
25.1
BLX07
19.0
13.0
17.1
16.7
21.7
19.1
21.0
20.0
BLX08
19.3
17.1
16.0
14.3
17.7
16.4
20.3
20.6
BLX09
ND
ND
1.1
ND
1.0
0.3
1.0
0.6
BLK10
ND
ND
ND
0.4
1.8
0.7
ND
0.5
BLK11
11.2
3.6
10.2
9.5
12.8
10.9
11.9
10.5
BLK12
10.5
8.6
10.0
9.5
10.4
9.1
13.8
9.8
BLK13
9.1
3.4
7.6
6.4
7.7
6.6
3.6
7.2
BLK14
0.4
0.4
1.0
1.1
0.5
0.5
1.4
0.7
BLX15
0.2
0.4
0.3
0.6
ND
ND
1.6
ND
3LK16
ND
ND
0.2
ND
ND
ND
0.6
0.5
BLK17
7.2
5.9
6.2
6.4
7.2
5.2
7.5
1.4
BLK13
9.4
7.9
9.3
8.1
7.3
7.0
10.0
6.7
BLK19
10.2
9.3
9.1
3.5
9.1
9.2
9.3
8.6
BLK20
9.6
9.3
9.9
8.9
10.0
3.3
10.3
9.2
BLK21
7.9
6.7
13.0
7.3
11.5
7.7
10.1
8.9
ND » Below the detection limit of 0.2 nq/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN. -
MILBURY RIVER
WWTP-
UBWPAD
en
LU
o
<
I—
o
60
50
40
30
20
10
0
D.
T
WEST
RIVER
NORTH- MUMFORO
BRIDGE RIVER ~~
WWTP
H
2 3 4 6
I
i
WOON
WWTP
BRANCH
RIVER
J 1—i 1 l L
50
45
40
T
A
1
35
8
T
A
i
11
T
A
i
A
12 13
17
18
19 20
T
21
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
A
A
A
-J 1 l. I
_J L— I - I .
30
25
20
10
T
i.
5
RIVER MILES
-------
1991 Blackstone River Survey #3
Total (TTM) and Dissolved (DTM) Nickel
in pq/L
Station
Run
.¥1
Run
¥2
Run
#3
[ Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLX01
5.4
4.6
2.3
2.0
4.3
4.3
4.7
3.2
BLKQ2
12.1
11.3
3.5
5.7
12.7
11.2
13.3
13 .4
BLX03
13.1
11.3
6.1
6.3
12.9
11.2
13.2
11.5
BLK04
11,2
10.5
9.3
5.3
11.6
10.7
11.1
10.7
BLK05
0.5
0.5
0.7
0.6
1.2
1.3
1.0
1.4
BLK06
3.4
6.2
3.4
6.6
9.2
7.9
8.3
6.8
BLK07
3.6
7.1
3.5
6.9
3.2
7.5
8.7
9.0
3LK08
3.6
7.6
5.2
3.6
9.1
8.2
9.6
3.3
BLK09
ND
ND
ND
ND
0.7
0.7
6.8
1.1
BLK10
ND
ND
ND
ND
0.3
0.5
1.9
0.7
BLK11
6.5
5.5
6.2
0.9
9.3
3.8
8.3
6.5
BLK12
5.9
4.3
3.7
ND
9.7
9.0
7.4
6.3
BLK13
6.1
4.9
2.7
0.5
10. 6
8.6
7.4
5.5
BLX14
1.2
1.5
1.3
1.5
4.6
3.5
2.0
1.2
BLK15
1.6
0.7
1.4
0.5
6.7
5.0
1.6
1.4
BLK16
0.5
0.8
2.2
0.7
5.1
3.8
1.2
1.1
BLK17
4.7
4.0
9.6
8.3
7.3
7.4
5.4
4.4
BLX18
6.0
3.7
6.4
4.9
7.3
7.3
5.1
4.2
BLK19
S.5
4.8
2.6
2.3
9.7
9.6
5.1
4.2
BLX20
5.5
4.3
2.3
2.4
11.7
12.6
4.9
5.1
BLK21
5.2
4.7
2.5
2.1
12.7
10.2
4.6
4.4
ND » Below the detection limit of 0.2 nq/Z*.
-------
Blackstone River
GRAFTON
WW1P
QUIN.
MILBURY RIVER
WWTP
UBWPAD
a r
WEST
RIVER
NORTH- MUMFORD
BRIDGE R,V£R ~~
WWTP
WOON.
WWTP
BRANCH
RIVER
1 2 3 4 6
8
1 1
12
13
17
18
19
20
21
LEGEND:
Survey III, October 2-3. 1991
Point Sources and River Inputs
Water Quality Stations. 1991
A
50
T
T
t*
45
40
A
35
T
A
1
30
T
A
T T
A A
i 1
25
20
T
A
i
A
15
t I
10
A
1
J .
5
RIVER MILES
-------
1991 Blackstone River Survey #1
Total (TTM) and Dissolved {DTM) Lead
in yg/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLX01
5.0
1.9
3.9
0.5
1.4
0.7
7.2
1.1
BLX02
3.5
0.6
3.2
ND
1.3
1.1
4.0
3.0
BLKQ3
3.5
0.4
2.9
0.5
1.0
0.6
3.3
2.4
BLX04
3.0
ND
4.1
0.4
3.3
2.1
5.0
0.2
BLK05
0.9
ND
1.9
ND
1.9
ND
2.3
2.3
BLK06
58.7
5.6
118
94.3
2.8
2.6
13.0
2.0
BLX07
16.7
1.6
274
5.3
98.4
4.7
12.9
5.2
BLK08
23 .6
1.8
16.9
3.0
4.8
4.2
32.5
0.9
BLK09
1.1
0.9
3.3
0.2
7.9
0.9
2.8
0.7
BLK10
3.4
0.3
2.2
1.2
3.6
1.6
29.6
1.3
BLX11
9.2
1.2
15.3
3.7
53.3
3.4
32.0
3.6
BLK12
S.l
2.3
19.6
1.3
2.9
2.6
6.1
5.7
BLK13
12.4
1.5
10.3
1.3
13.2
2.1
15,7
0.3
BLX14
23.5
21.2
1.6
1.5
1.0
0.8
2.0
0.2
BLK15
1.7
0.3
1.0
ND
0.5
1.7
1.7
0.4
BLK16
9.4
5.7
3.5
1.3
12.3
3.4
3.9
1.4
BLX17
3.7
0.5
4.0
ND
4.5
2.3
4.7
0.4
BLK1S
4.3
0.2
3.2
ND
3.4
2.0
4.2
ND
BLK19
2.2
ND
2.0
ND
2.5
0.8
2.5
0.3
BLK20
2.0
2.1
1.7
ND
1.6
1.5
l.S
ND
BLK21
2.7
0.3
3.3
NO
4.9
1.6
3.4
0.5
ND » Below the detection limit of 0.2 Jig/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN. -
MILBURY RIVER
WWTP-
UBWPAD
_3
WEST
RIVER
NORTH- MUMFORD
BRIDGE R,VER ~
WWTP
WOON
WWTP
BRANCH
RIVER
I I I I
T
rf
50
3 4
6 7 8
A A
4B.t+/- 52.5 101 +/- 122
A
£
1 1
A
12
A
13
I
t
17
18
19
20
21
LEGEND:
: Survey I, July 10-11,1991
— : Point Sources and River inpuls
1 : Water Quality Stations, 1991
A
I I I L_
A
_j ,
A
A
A
45
40
35
30
25
20
15
10
o
RIVER MILES
-------
1991 3iackstone River Survey $2
Total (TTM) and Dissolved (DTM) Lead
in nq/L
Station
Run #1
Run ¥2
Run #3
Run .#4
| TTM
dtm
TTM | DTM
TTM | DTM
TTM DTM
BLX01
4.6
2.5 |54.3 ) 4.5
19.7
4.5
5.7
4.2
BLK02
4.3
3.1
6.7
2.4
18.7
15.0
3.7
2.5
BLK03
3.5
1.2
3.3
3.2
25.7
22.7
3.2
1.2
BLK04
13.4
4.0
4.7
4.0
6.9
6.6
2.5
1.5
BLK05
6.0
6.1
3.3
3.3
6.2
2.1
0.5
ND
BLK06
9.2
7.2
17.0
11.9
21.3
12.3
6.7
3.9
BLK07
11.7
5.7
13.0
11.5
32.0
21.0
6.4
4.6
BLK08
25.6
13.0
20.4
9.4
37.2
24.5
18.8
11.0
BLX09
1.1
0.4
6.1
4.9
5.1
3.2
1.0
ND
BLX10
1.6
2.0
4.3
3.8
6.3
4.6
1.1
1.3
BLX11
11.4 | 4.2
11.0
9.2
26.9
17.0
7.8
4.3
BLX12 | 6.5
2.2
19.2 J 15.7
13.4
6.0
5.7
3.0
BLK13
8.2
5.5
3.5
10.3
41.2
35.1
6.2
0.3
BLK14 2.8
1.2
2.5
1.3
1.9
0.9
3.1
1.1
3LX15
2.1
1.1
48.8
0.3
1.1
0.2
4.1
0.2
BLK16
2.9
1.7
2.0
0.8
3.9
1.7
8.0
4.3
BLX17
5.0
1.5
4.5
1.3
5.8
1.5
10.2
2.5
BLK13
6.6
5.5
4.5
2.6
4.6
3.5
6.9
1.3
BLK19
3.9
2.5
7.2
5.0
5.8
5.1
6.6
ND
BLX20
3.5
2.3
3.4
0.4
5.0
2.1
NO
ND
BLK21
3.8
2.9
4.9
2.2
5.0
0.7
ND
ND
ND
Below tiie detection limit of 0.2 iiq/L.
-------
Blackstone
River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP-
UBWPAD
ZL_
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~
WWTP
WOON.
WWTP
BRANCH
RIVER
1 ^ 2
T
A
1
50
3 4
1 1
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
I
tA
I
1 t
_l 1 1 L-
45
40
35
30
-J 1 ' '
25
12
1J
* f
1 7
A
20
18
19 20
A
A A
T
A
L
_l 1 L
15
10
RIVER MILES
-------
1991 Blacfcstone River Survey #3
Total (TTM) and Dissolved (DTM) Lead
in ng/L
Station
Run
*1
Run
$2
Run
$ 3
Run
#4
TTM
DTM
TTM
DTM
TTM
DTM
TTM
DTM
BLK01
2.6
0.4
4.3
0.9
6.0
2.9
7.6
1.4
BLK02
2.6
0.9
3.3
1.3
4.8
2.0
3.9
2.7
BLK03
2.7
0.3
2.9
0.6
9.7
1.4
4.7
1.4
BLK04
3.9
2.4
3.5
1.5
4.9
1.9
4.3
2.3
BLK05
2.1
0.7
1.4
0.7
0.8
0.4
1.1
1.0
BLK06
8.3
2.3
7.7
2.3
33.6
10.8
6.3
1.7
BLK07
9.8
6.1
6.7
2.9
8.8
2.7
7.0
3.3
BLX08
10.2
3-7
7.7
2.8
8.6
2.5
13.1
5.8
BLX09
2.4
2.4
5.1
1.1
4.9
1.0
4.3
0.6
BLK10
4.6
2.4
2.9
0.9
3.2
1.7
2.4
0.9
BLK11
8.5
5.2
13.1
1.3
9.4
3.4
8.0
1.8
BLK12
7.4
6.7
10.9
1.5
10.8
6.2
7.6
1.7
BLK13
8.2
3.0
9.7
1.8
14.4
3.8
6.9
1.4
BLK14
6.8
5.9
1.3
1.2
1.5
0.7
1.8
0.3
8LK15
3.3
2.5
1.2
0.8
2.3
0.4
1.2
0.5
BLK16
2.5
2.1
3.4
1*9
1.2
1.2
5.0
0.4
BLX17
11.3
9.4
6.6
1.0
4.4
1.4
14.8
2.3
BLK18
9.6
3.1
6.5
1.7
4.7
1.1
29.8
15.3
BLK19
3.7
2.2
4.4
1.5
3.6
1.1
5.7
1.4
BLK20
3.8
0.9
3.9
1.0
5.5
2.3
4.4
1.7
BLX21
5.1
2.1
3.0
1.2
3.0
1.1
4.6
1.9
MD » Below the detection limit of 0.2 nq/L.
-------
Blackstone
River
GRAFTON
WWTP
cn
Q
<
Ld
<
h-
o
55
50
45
40
35
30
25
20
15
10
5
0
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n
-1
A
A
WEST
RIVER
NORTH— MUMFORD
BRIDGE R,VER ~
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
8
1 1
LEGEND:
: Survey III, October 2-3, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
A
A
A
A
A
I
12 13
A
A
' t i t » ¦ L
_l—» t I i i j—u_
50
45
40
35
30
25
17
20
18
f *
i l .
15
T
A
1
19 20
A
21
T
f
i._. L.
10
RIVER MILES
-------
1991 Blackstone River Survev ^
Five Day 3iochejnic-»A Demand in mg/L
Station
Run .#1
Run #2
Run #3 j Run #4
BLX01
ND
ND
1.2
1.4
BLX02
1.0
1.3
2.0
2.6
BLX03
1.7
1.2 | 1.1
1.3
BLK04
ND
ND
1.6
1.4
3LK05
ND
ND
ND
ND
BLK06
ND
ND
l.S
1.4
BLK07
1.2
1.7
3.1
2.4
ELK 08
1.6
2.6
4.3
2.0
BLK09
ND
ND
ND
1.0
BLK10
ND
ND
1.0
ND
BLK11
1.5
2.9
3.3
3.4
BLK12
1.9
3.5
4.3
3.4
BLK13
1.7
2.8
3.0
2.6
BLK14
ND
1.0
1.1
1.3
BIiKIS
ND
1.5
l.S
BLK16
ND
ND
1.2
1.6
BLK17
1.3
2.7
3.4
3.0
BLK18
2.7
3.3
3.9
3.4
BLK19
2.4
4.1
4.5
3.5
BLK20
1.3
1.7
2.3
2.4
BLX21
1.3
2.2
2.7
2.2
ND = Below the detection limit of 1.0 mg/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
H
8
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER
WWTP
WOON
WWTP
BRANCH
RIVER
lO
Q
O 3
CD
0
f
A
3 4
A
A
A
it 1
't 1
-J I t I 1 1 I 1 L
_J lit! I-
12 13 17 18 19 20 21
LEGEND:
Survey I, July 10-11,1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
A
_i l .1 L I 1 J 1 L
50
45
40
35
30
i- j_
25
A
z„\
A
I 1
A
A 1
_l J J 1 1 I 1 | L i I I I .. . 1..
20
10
RIVF'R MILES
-------
1991 31ac]
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MIL8URY RIVER
WWTP -
UBWPAD
ZL_
8
i n
Q
O 3
WEST
RIVER
NORTH- MUMFORD
BRIDGE 51VER
WWTP I
WOON
WWTP
BRANCH
RIVER
1 2 3 4 6
LEGEND:
Survey II, August 14—15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
A
A
A
A
A A
A
_J l .1 i„,
50
J i J-1
45
12 M 17 18 19 20 21
A
A
-a l i 1— l l i J i _
40
35
30
25
1 I I ll-
A
A
A
A
-i 1 1_. a j i-
20
-_l L . . I - 1— .J J
10 s
RIVER MIIES
-------
1991 Blackstone River Survey iZ
Five Day 3iochenicai Oxygen Demand in mg/L
Station
Run #1
Run #2 j Run #3
Run #4
BLKQl
1.4
l.S
ND
1.5
BLK02
.1.3
1.3
1.3
1.0
BLX03
1.1
2.0
1.5
1.4
BLK04
1.5
1.3
1.3
1.7
BLK05
ND
1.2
ND
ND
BLX06
1.1
1.3 | 1.7
1.4
BLXQ7
1.5
1.8 | 1.1
ND
BLX03
1.1 -
1.8
1.2
ND
BLX09
ND
1.4
ND
ND
BLK10
ND
1.1
ND
ND
BLK11
ND
1.3
ND
ND
BLK12
ND
1.1
ND
ND
BLK13
1.1
ND
1.2
ND
BLX14
1.4
ND
ND
ND
BLK15
1.0
ND
ND
1.2
8LK16
.1.1
1.2
ND
ND
BLX17
ND
1.0
ND
1.2
BLX13
ND
1.0
ND
1.2
BLX19
1.5
1.0
ND
1.3
BLK20
1.1
ND
ND
1.7
BLK21
2.4
ND
2.4
3.7
ND ® Below tiie detection limit of 1.0 ag/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN. -
MILBURY RIVER
WWTP -
UBWPAO
ZL
8
cn
£ 4
lO
Q
O 3
m
o
50
A
T
£
WEST
RIVER
NORTH-
BRIDGE
WWTP
MUMFORD
RIVER —
WOON
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
T
A
r1 t
T
A
A
I
A
1
45
40
35
30
12
25
13
T I
^ f
17
T
18
19 20
T
21
20
A
i 5
7
A
1
A
10
RIVER MILES
-------
1991 Blackstone River Survey #1
Calcium in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
22.4
23.6
NS
23.7
BLX02
19.2
18.4
20.9
18.1
BLK03
20.3
19.2
10.3
0
CO
1—I
BLK04
22.3
18.6
19.0
17.8
BLK05
16.9
14.3
15.1
13.8
BLK06
18.0
17.6
17.4
16.9
BLK07
16.6
15.8
16.0
15.1
BLK08
15.5
15.9
16.0
13.9
BLK09
6.3
4.1
5.4
5.2
BLK10
6.6
6.1
5.8
5.8
BLK11
12.8
13.1
13.1
12.6
BLK12
13.6
12.7
12.6
12.2
BLK13
12.8
10.8
10.8
11.4
BLK14
3.4
4.1
3.9
4.3
BLK15
6.7
6.6
6.6
7.1
BLK16
9.8
9.7
10.2
9.9
BLK17
12.2
11.1
11.2
11.5
BLK18
12.7
11.1
12.1
11.4
BLK19
12.0
11.6
11.5
11.7
BLK20
11.9
12.1
11.9
11.6
BLK21
14.0
11.4
11.4
12.2
ND » Below the detection Limit of 0.1 mg/L; NS ¦
Sample missing.
-------
Blocks tone River
GRAFTON
VYWTP
QUIN. -
MIL8URY RIVER
WWTP-
UQWPAO
-IL
1 2 3 4 6
A
50
T
¦f
rrn
WES1
RIVER
NORTH- MUUrORO
BRIDGE R,VCR
WOTP
WOQN
WWTP
¦ T
A
A
45
40
BRANCH
RWER
1 I
A
A
i
35
30
II 1
12
1 7
19
20
21
LEGEND:
: Survey I, July 10- 11,1991
— : Point Sources and River Inputs
1 : Wafer Quality Stations, 1991
T T
4 A
1 J-
A
A
-J 1—
20
1 5
10
KlV'f MILLS
-------
1991 Blackstone River Survey #2
Calcium in mg/L
station
Run #1
Run #2
Run #3 J Run #4
BLK01
18.8
19.5
29.3
19.0
BLKQ2
18.0
15.7
17.5
17.1
BLK03
18.7
16.4
16.2
16.6
BLK04
17.1
16.7
17.0
16.1
BLX05
14.3
12.3
13 .0
12.9
BLX06
16.1
14.9
15.3
16.4
BLK07
14.7
13.6
14.9
16.4
BLK08
13.0
13.7
13.4
13.4
BLX09
S. 6
5.4
4.6
4.3
BLK10
S.8
5.3
5.2
5.1
BLK11
11.0
11.2
11.0
10.8
BLX12
11.4
10.1
10.6
11.0
BLK13
9.6
8.7
3.6
10,1
BLK14
4.7
7.3
4.2
3.9
BLX15
6.6
6.1
6.0.
6.0
BLK16
8.7
7.6
8.5
8.2
BLK17
9.2
9.0
9.0
8.6
3LK18
10.7
10.1
9.7
9.3
BLK19
11.4
11.2
10.4
10.1
BLK20
12.4
12.3
11.4
11.2
BLX21
11.5
11.6
11.7
11.2
ND = Below the detection limit of 0.1 mg/L.
-------
Blackstone River
GRAFFON
WW1P
30
25
QUIN.
milbury river
WWTP-
U6WPAD
a_
CT>
£ 20
15
10
0 L
A
WEST
RIVER
NORTH- MUMFORD
BRIDGE R,VER —
WWTP
45
1 2 3 4 6
M4 ^
_j k—i i_
40
WOON.
WWTP
BRANCH
RIVER
T
A
1
A
35
11
A
A
—i- j 1 l_
30
25
12
13
17
18
19
20
21
LEGEND:
Survey II, August 14-15, 1991
Point Sources arid River Inputs
Water Quality Stations, 1991
A
A
A
A
£
_l L 1 L-
_l J 1 X..
20
15
10
_.i
(I
(i
RIVER MILLS
-------
1991 Blackstone River Survey #3
Calcium in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
14.4
14.2
14.8
14.2
BLK02
15.9
16.3
16.5
15.5
BLK03
15.6
15.2
15.0
15.8
BLK04
15.2
14.7
14.9
14.5
BLXOS
11.8
11.4
11.9
11.7
BLK06
14.1
13.5
14.3
14.4
BLK07
13.2
13.4
13.7
12.7
8LK08
12.3
13.2
12.9
12.8
BLK09
3.8
3.6
3.2
3.1
BLK10
4.1
4.1
4.4
4.2
BLK11
9.9
9.8
9.1
9.4
BLK12
9.7
3.9
8.9
9.9
BLK13
9.7
8.9
9.4
9.1
BLK14
2.4
2.4
2.6
2.5
BLK15
5.1
5.0
5,2
4.8
BLK16
7.5
7.3
7.4
7.9
BLK17
7.9
8.0
7.9
8.3
BLK13
7.8
7.1
7.4
7.7
BLK19
8.0
8.0
8.2
8.4
BLK20
8.3
8.7
8.5
8.9
BLK21
3.1
8.5
8.2
8.1
ND » Below the detection limit of 0.1 mg/L.
-------
Blockstone River
GRAFTON
WWTP
30
25
20
15
10
QUIN.
MILBURY RIVER
WWTP
UBWPAD
3
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~
WWTP
WOON
WWTP
BRANCH
RIVER
CP
E
13
_U
o
o
o
o
A
0
50
A
3 4
1 1
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
A
A
A
A
45
¦ I
40
-1 L I X-
35
30
25
A A
12 13 17 18 19 20 21
A
A
A A . A
20
10
RIVffR MILES
-------
1991 BlacJcstone River Survey #1
Magnesium in mg/L
station
Run.#1
Run #2
Run #3
Run #4
BLK01
3.88
3.82
NS
3.91
BLK02
3.56
3.35
3 .66
3.79
BLK03
3.37
3.37
3.48
3.57
BLK04
3.45
3.31
3 .58
3.40
BLKQ5
2.78
2.73
2.92
2.91
BLK06
3.13
2.32
3 .48
3.47
BLK07
3.07
3.12
3.31
3.13
BLK08
3.03
3.04
3.09
3.31
BLX09
1.25
1.20
1-20
1.21
BLK10
1.32
1.34
1.35
1.37
BLK11
2.43
2.64
2.81
2.71
BLK12
2.47
2.55
2.64
2.57
BLK13
2.37
2.21
2.32
2.41
BLK14
1.05
1.05
1.06
1.09
BLK15
1.56
1.50
1.56
1.66
BLK1S
2.0 8
2.22
2.28
2.30
BLK17
2.13
2.24
2.31
2.41
BLK13
1.91
2.37
2.48
2.38
BLK19
2.31
2.32
2.44
2.45
BLK20
2.45
2.39
2.49
2.53
BLK21
2.53
2.39
2.70
2.54
ND » Below the detection limit of 0.05 ag/L; NS »
Sample missing.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
3
WEST
RIVER
NORTH— MUMFORD
BRIDGE RIV£R ~
WWTP
WOON
WWTP
BRANCH
RIVER
A
A , -
l A A
2 3 4 6
A
A
1 1
A
i i r
12 13
17
18
19 20
2 1
A
1 :
LEGEND:
Survey I, July 10-11, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
I
i
A
A
A
A
0
till i i
50
45
' ' ' ¦ I 1 1 1 I 1 1 1 » 1
I 1 I ft 1 1 1 I i 1-.1_
40
35
30
20
15
—J 1 j i
-.1—.
10
0
RIVER MILES
-------
1991 Blackstone River Survey #2
Magnesium in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
2.76
2.74
2.67
2.85
BLK02
3.26
2.89
2.93
3.08
BLK03
3.29
3.02
2.99
3.15
BLX04
3.27
3.05
2.93
2.97
BLKOS
2.73
2.65
2.61
2.78
BLK06
3.06
2.93
2.98
2.85
BLK07
2.65
2.44
2.75
2.61
BLK08
2.51
2.23
2.68
2.71
BLK09
1.22
0.92
1.10
1.05
BLK10
1.38
1.13
1.21
1.22
BLK11
1.97
1.87
1.99
2.08
BLK12
1.99
1.75
1.88
1.79
BLK13
1.75
1.47
1.79
1.91
BLK14
1.06
0.95
1.08
1.09
BLK15
1.48
1.27
1.42
1.43
BLK16
2.00
1.80
1.92
1.87
BLK17
1.76
1.53
1.83
1.89
BLX13
2.06
1.75
1.90
1.88
BLK19
2.18
1.90
2.01
2.15
BLK20
2.34
2.08
2.22
2.29
BLK21
2.25
2.07
2.13
2.17
ND Below the detection limit of 0.05 mg/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN. ¦
MILBURY RIVER
WWTP-
UBWPAO
a
WEST
RIVER
NORTH— MUMFORD
BRIDGE RIVER
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
1 1
12 13
17
18
19 20
LEGEND:
: Survey II, August 14-15, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
A
T
A
1
A
A
A
4
I
£
A
A
A
50
45
40
35
30
25
20
15
10
. i i
. i .
0
RIVER MiL.ES
-------
1991 Blackstone River Survey #3
Magnesium in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
3.00
3.12
2.67
2.89
BLK02
3.70
3.43
3.06
3.28
BLK03
3.30
3.57
3.63
3.54
BLK04
3.50
3.12
2.39
2.93
BLK05
2.21
2.44
2.79
2.64
BLK06
3.19
2.86
2.53
2.84
BLK07
2.71
2.84
2.89
2.78
BLK08
2.89
3.15
3.53
3.24
BLX09
1.19
1.38
1.34
1.21
BLK10
1.62
1.84
2.01
1.84
BLK11
2.45
2.52
2.43
2.56
BLK12
2.22
2.35
2.30
2.43
BLK13
2.19
2.10
1.98
1.95
3LK14
0.69
1.06
1.03
0.94
BLK15
1.99
1.85
1.77
1.79
BLK16
2.2S
2.74
2.78
2.54
BLK17
1.93
1.88
1.78
1.80
BLK18
2.40
2.48
2.30
2.51
BLK19
2.12
2.21
2.15
1.95
BLK20
2.06
1.95
1.87
1.84
BLK21
2.44
2.50
2.41
2.56
ND ¦ Below the detection limit of 0.05 mg/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN. ¦
MIL8URY RIVER
WWTP-
UBWPAD
n
50
T
A
x
T
A
i
west
RIVER
NORTH- MUMFORD
BRIDGE RIVER
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey 1(1, October 2-3, 1991
Point Sources ond River Inputs
Water Quality Stations, 1991
£
I
A
1
T
A
I
I
I
A
A
_J » 1 L_
45
40
35
30
12 13 17
A
A
-I _ I. ... I- . I-
25
20
18
A
15
19 20
A
A
A
-_J I- 1_ .1 _
. i
10
<)
RIVER MILES
-------
1991 Blackstone River Survey #l
Total Chlorophyll in nq/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
ND
2.8
BLX02
2.0
1.9
BLK03
2.1
2.3
BLK04
1.0
2.0
BLK05
NA
NA
BLX06
3.2
3.7
BLK07
6.2
22.0
BLK08
17.0
20.6
BLK09
NA
NA
BLK10
NA
NA
BLK11
12.1
19.6
BLK12
21.0
13.0
BLK13
17.8
20.6
BLK14
NA
NA
BLK15
NA
NA
BLK16
2.4
4.8
BLK17
NA
25.2
BLK13
15.0
29.5
BLK19
16.2
29.1
BLK20
3.2
8.6
BLK21
13.1
11.3
ND » Below the detection limit of 1.0 ng/l>.
-------
Blackstone
River
GRARON
WWTP
140
120
GUIN
MIL BURY RIVER
WWTP
UBWPAD
cn 100
80
60
40
20
0
V
NORTH-
BRIDGE
WWTP
WEST
RIVER
MUMfORO
RIVER —
M
WOON
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
A : Survey I, July 10-11,1991
— : Point Sources and River Input;
1 . Water Quality Stations, 1991
A
A
A ¦ . A i A j !eLj i i i_
A
50
45
40
.30
A
A
A
-U I
25
20
A
A
12 13 17 18 19 20 2
A
I 5
T
A
l j.L. t— i k _i. l ,t
10
O
RIVLR MILES
-------
1991 Blackstone River Survey #2
Total Chlorophyll in m
-------
Blctckslone River
GRAFTON
wwtp
QUIN -
MIL8URY RIVER
WWTP -
U6WPAD
140
120
100
80
60
40
20
0
It Li
WEST
RIVER
NORTH- MUMFORO
BRIDGE R,VER ~~
WWTP
1 2^4 6
LEGEND:
A : Survey II, August 14—15
— : Point Sources and River
1 : Water Quality Stations, 1
WOON
WWTP
BRANCH
RIVER
A
T
4r* i i A—I—iA.i y\—i——i—i—i—!_
A
i
_i Li I L-
T
A
50
45
40
35
30
25
A
I I
12 13 17
A
>0
18
A
19 20
21
_J l i-J L
A
15
L__
10
A
j
f:
I }
RIVER MILES
-------
1991 Blackstone River Survey #3
Total Chlorophyll in nq/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
2.3
1.0
BLK02
2.9
1.1
BLX03
1.0
ND
BLX04
1.0
1.1
BLK05
2.3
4.7
BLK06
2.0
3.2
BLK07
ND
3.1
BLK08
2.8
2.0
BLK09
2.0
1.0
BLK10
1.0
3.3
BLK11
ND
1.9
BLK12
2.0
ND
BLK13
3.7
1.0
BLK14
2.0
4.0
BLK15
4.0
6.0
BLK16
ND
ND
BLK17
2.9
3.1
BLK18
2.9
1.0
BLK19
1.1
2.0
BLK20
2.8
2.0
BLK21
2.1
4.0
ND = Below the detection limit of 1.0 ng/L.
-------
Blackstone River
GRAfTON
WWTP
140
20
100
80
60
40
20
0
OUtN -
MfLBURY RIVER
WWTP
UBWPAD
jO
A
iA
WEST
RIVER
NORTH-
BRIDGE
WWTP
MUMFORD
RIVER —
W \l
WOON.
WWTP
BRANCH
RIVER
3 4
1 I
LEGEND
Survey III, October 2-3, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
I T I
2+^ I——i—^ i
_^A.
T
T
1
V
13
17
18
45
40
35
30
. u^c I i <_£a
25 20 15
I I I
19 20
21
L.,1.
10
..til:
I.
I )
RIVER MILES
-------
1991 Blaclcstone River Survey #1
Chloride in ag/L
Station
Run #1
Run # 2
Run #3
Run #4
BLK01
114
114
114
148
BLK02
109
109
NS
100
BLK03
104
105
92
100
BLKQ4
97
105
92
100
BLK05
67
73
65
68
BLK06
82
82
74
92
BLK07
78
82
68
78
BLK08
75
79
71
74
BLK09
20
22
19
19
BLX10
46
43
35
43
BLK11
67
75
63
65
BLK12
70
75
60
65
BLX13
57
64
50
60
BLK14
22
24
20
21
BLK15
24
24
20
24
BLK16
34
36
30
48
BLK17
57
59
48
57
BLK13
79
79
65
68
BLK19
64
70
68
71
BLK20
52
57
50
63
BLK21
54
35
46
55
ND » Below the detection limit of 1 mg/L; NS ¦
Sample missing.
-------
Blacks-tone River
GRAFTON
WWTP
200
180
160
140
cn
£ 120
UJ 100
Q
o 80
x 60
40
20
QUIN.
MILBURY RIVER
WWTP"
UBWPAD
a_
T
A
1
T
A
1
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~
WWTP
W00N
WWTP
BRANCH
RIVER
1
3 4
1 1
LEGEND:
Survey I, July 10—11,1991
Point Sources and River Inputs
Water Quality Stations, 1991
I
f
T
t
* A
i
0 L-*—¦. . . 1
50 45
I
A
1
-J I 1 i—J—-J-—J 1- I l„ ¦ I .
40
35
30
—i—i I
25
12
13
T
A
1
20
17
18
I
I
i
15
19 20
A
T
A
I.
10
RIVER MILES
-------
1991 Blackstone River Survey #2
Chloride in mg/L
Station
Run #1
Run 12
Run $2
Run #4
BLK01
108
97
106
116
BLK02
105
90
96
113
BLK03
100
94
99
109
BLKQ4
96
90
95
108
BLK05
72
65
69
78
BLK06
90
77
85
100
BLX07
78
79
78
156
BLK08
76
72
72
140
BLK09
10
19
21
21
BLX10
45
41
42
49
BLK11
56
51
57
66
BLK12
57
43
53
62
BLK13
48
42
42
55
BLK14
30
28
26
29
BLK15
28
25
26
29
BLK16
38
32
35
40
BLK17
48
42
44
236
BLK13
62
54
54
60
BLK19
60
53
54
68
BLK20
68
60
60
68
BLK21
64
56
60
50
ND = Below the detection limit of l mg/L.
-------
Black stone River
GRAFTON
WWTP
200
180
160
HO
CP
£ 120
UJ 100
Q
9^ 80
60
40
20
0
QUIN.
MILBURY RIVER
WWTP-
UBWPAD
n
A
I
A
WEST
RIVER —
NORTH- MUMFORD
BRIDGE RIVER ~~
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
II
1
A
A
T
A
x
T
A
i
50
45
40
35
30
T
A
1
25
T
A
1
12 13 17
18
19 20
.21
T
A
1
T
I
A
1
A
L
A
20
15
10
RIVER MILES
-------
1991 Blackstone River Survey #3
Chloride in ing/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
57
59
74
60
BLK02
71
78
93
81
BLK03
70
72
88
79
BLK04
70
68
82
94
BLKOS
48
47
70
53
BLK06
66
61
71
59
BLX07
58
62
71
62
BLX08
58
59
69
62
BLK09
14
15
13
18
BLK10
26
27
29
28
BLX11
45
46
51
48
BLX12
44
45
50
48
BLK13
44
43
48
39
BLK14
12
14
14
13
BLK15
28
28
31
28
BLK16
26
27
29
34
BLK17
36
38
43
41
BLK13
40
40
48
46
BLK19
40
40
46
46
BLK20
NS
52
43
55
BLK21
49
41
44
54
ND = Below the detection limit of 1 mg/L.
-------
Blackstone River
GRAF'TOIM
WWTP
200
180
160
140
120
100
80
60
40
20
QUIN.
MILBURY RIVER
WWTP
uewpAO
zi
n rr
1 2 3 4
WEST
RIVER
NORTH- MUMFORD
BRIDGE R,VER "
WWTP
WOON
WWTP
BRANCH
RIVER
T
t
7
8
1 1
1 :
LEGEND;
Survey III, October 2 — 3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
ill
T
A
1
T
A
1
T
A
1
A
A
45
. i
40
35
¦ I
30
_L_
25
12 13
T
A
X
1 7
A
18
T
A
I t -.
20
15
19 20
T
A
A
T
_ .J—i—i — i. —i.. _ L a
10 ^
RIVLR MILES
-------
1991 Blackstone River Survey #1
Ammonia in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLKQ1
0.14
0.17
0.24
0.24
SLX02
0.48
0.26
0.24
0.45
BLK03
0.29
0.21
0.11
0.18
BLK04
0.38
0.29
0.28
0.33
BLK05
0.07
0.07
ND
0.08
BLK06
0.12
0.13
0.12
0.16
BLK07
0.21
0.15
0. 05
0. 08
BLKOS
0.12
0.05
0.14
0.11
BLX09
0.09
0.04
NO
0.06
BLK10
0.04
0.04
0.04
0.07
BLK11
0.06
0.06
0.10
0.02
BLK12
0.04
0.05
0.05
0.06
BLK13
Q. 05
0.06
o.os
0.09
BLK14
0.09
0.12
0.08
0.09
BLK15
0.06
0.04
0.05
0.05
BLK16
0.22
0.26
0.25
0.33
BLK17
0.06
0.05
0.05
0.09
BLK18
1.03
1.54
1.01
1.07
BLK19
1.03
0.61
0.77
0.99
BLK20
0.55
0.50
0.43
0.45
BLK21
0.33
0.31
0.36
0.25
ND = Below the detection limit of 0.02 mg/L.
-------
Blacl
-------
1991 Blackstone River Survey #2
Ammonia in mg/L
Station
Run #1
Run #2
Run #3
Run 0 4
BLK01
1-37
0.65
ND
ND
BLK02
1.62
0.73
ND
ND
BLKQ3
1.66
0.75
0.03
ND
BLK04
1.72
1.13
0.02
ND
BLK05
0.49
0.32
0.09
ND
BLK06
1.21
0.58
0.69
ND
BLX07
0.53
0.93
0.42
ND
BLKQ8
0.13
0.78
0.32
ND
BLK09
0.26
0.46
0.15
ND
BLK10
0.55
0.18
0.13
0.13
BLK11
0.09
0.41
0.40
0.26
BLK12
0.13
0.33
0.37
0.27
BLK13
0.08
0.38
0.34
0.26
BLK14
0.22
0.62
0.25
ND
BLX1S
0.09
0.26
0.26
0.39
BLK16
0.20
0.28
0.17
0.18
BLK17
ND
0.22
0.10
0.14
BLX13
0.83
1.25
0.22
0.74
BLK19
0.67
0.43
0.75
0.83
BLK20
0.49
0.34
0.04
0.43
BLK21
0.50
0.44
0.64
0.04
ND ¦ Below the detection limit of 0.02 ag/L.
-------
Blacks tone River
GRAe TON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
11
2
Cn
<
21
O
<
T
A
1
A
0 L-
50
west
RIVER
NORTH- MUMEORD
BRjDGE R|VER
WWTP
WOON
WWTP
BRANCH
RIVER
3 4
1 1
12
13
1 7
18
19 20
21
LEGEND:
Survey II. August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
1
T
A
i
A
A
I
A
1
T
i 4
A
A A
T
A
1
1
f-
A
45
40
30
25
20
15
¦ . i
10
RIVER MILES
-------
1991 Blackstone River Survey #3
Ammonia in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
0.15
0.11
0.15
0.17
BLK02
0.19
0.07
0.75
' 0.54
BLX03
0.16
0.14
0.24
0.56
BLK04
0.18
0.20
0.32
0.46
BLK05
ND
ND
ND
ND
BLX06
0.21
0.13
0.40
0.16
BLK07
0.14
0.23
0.23
0.24
BLK08
0.07
0.15
0.21
0.18
BLK09
ND
ND
ND
0.03
BLK10
ND
ND
ND
ND
BLK11
0.07
0.09
0.10
0.09
BLK12
0.08
0.12
0.09
0.12
BLK13
0.08
0.10
0.19
ND
BLX14
0.02
0.04
0.07
ND
BLK15
0.06
0.03
0.11
0.08
BLK16
0.06
0.06
0.05
0.04
BLK17
ND
0.77
0.10
0.05
BLK13
0.1S
0.16
0.33
0.22
BLK19
0.21
0.20
0.23
0.21
BLK20
0.17
ND
0.21
0.17
BLK21
0.14
0.20
0.25
0.15
ND ¦ Below the detection limit of 0.02 mg/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP -
UBWPAD
a
WEST
RIVER
NORTH- MUMFORD
BRIDGE R,VER ~~
WWTP
WOON
WWTP
BRANCH
RIVER
A
T
3 4
13
1 7
19
20
21
LEGEND:
Survey III, October 2-3, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
?
7
A
1
A
I
A
T
A
x
~
45
40
35
30
A
A A
A ,
...X i J J
A
T A
t 1
20
I 5
I (J
RIVfR MILKS
-------
1991 Blackstone River Survey /l
Nitrate in mg/L
Station
Run #1
Run K
Run #3
Run #4
BLK01
0.65
0.51
1.00
0.45
BLK02
2.90
2.57
4.55
2.02
BLK03
4.03
3.64
4.16
3.89
BLK04
4.43
4.20
4.42
4.47
BLK05
0.13
0.08
0.20
4.05
BLX06
2.78
2.64
4.42
0.07
BLKQ7
2.74
2.85
3.63
3 .68
BLK08
2.37
2.29
2.79
3.10
BLK09
0.15
0.14
0.15
3.18
BLK10
0.12
0.03
0.10
0.17
BLK11
1.70
1.52
2.66
1.74
BLK12
1.77
1.39
1.92
1.65
BLX13
1.57
1.50
1.73
1.46
BLK14
0.24
0.22
ND
0.27
BLK15
0.38
0.29
0.20
0.39
BLK16
0.76
0.81
NS
0.77
BLK17
1.28
1.20
0.31
1.46
BLK18
1.23
1.17
1.47
1.41
BLK19
1.40
1.38
1.57
1.62
BLK20
1.64
1.69
1.74
1.98
BLK21
1.59
1.98
2.02
1.90
ND » Below the detection limit of 0.02 mg/L; NS
= Sample missing.
-------
Blockstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n .
\ 6
cn
£ 5
I lJ
<
cr
3
2
0
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~
WWTP
WOON.
WWTP
BRANCH
RIVER
1 2 3 4 6
8 1 1
LEGEND:
Survey I, July 10-11,1991
Point Sources and River Inputs
Water Quality Stations, 1991
I
A
A
A
T
A
1
A 1. L- I— I i. It _ I .1. I.— I I
I
A
1
A
T
$ i
I I
12 13 17 18 19 20 21
A
~
A
A
—I 1 1 L J i t I I - I 1 L I I J J.— 1
T
A
L
50
45
40
35
30
25 20
15
i .... i
10 5
i -i . t... i
0
\IVi: R MILES
-------
1991 Blackstone River Survey #2
Nitrate in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
0.16
1.45
0.71
0.66
BLK02
2.70
3.81
4.18
4.77
BLK03
4.29
3.63
3.57
5.21
BLK04
4.09
4.21
3.35
4.20
BLK05
0.03
0.14
0.13
0.11
BLK06
3.42
2.89
4.05
3.37
BLK07
4.20
2.54
4.24
2.86
BLK08
2.85
4.29
3.80
2.86
BLK09
2.46
0.14
0.04
0.09
BLK10
0.98
0.19
ND
0.09
BLK11
1.41
1.45
2.00
1.20
BLK12
4.52
1.60
2.38
1.32
BLK13
0.97
0.82
2.38
0.95
BLK14
0.25
0.28
1.25
0.31
BLK15
0.25
0.25
0.17
0.89
BLK16
0.56
0.65
0.68
0.95
BLK17
0.79
0.82
0.81
1.14
BLK13
1.43
1.17
4.65
1.52
BLK19
1.76
1.51
1.69
1.97
BLK20
2.03
1.74
1.99
2.29
BLK21
1.67
1.63
1.72
2.41
ND = Below the detection limit of 0.02 mg/L.
-------
Blackstone River
GRAFTON
WWTP
QIIIN. ¦
MIL0URY KlVER
WWTP-
UBWPAD
n_
1WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~
WWTP
WOON
WWTP
BRANCH
RIVER
8
7
0
A
A
3 4
11
LEGEND:
Survey II, August 14-15,1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
A
I
A
1
A
A
A
I
¦ I i i i 1 1 1 1—
I ' j » 1 I 1 J 1 A 1
12 13 17
A
50
45
40
35
30
A
25
T
A
l
T
A
1
T
A
1
19 20
T
A
1
-L I 1 i 1 1 L
20
15
....i
10
21
A
RIVER MILES
-------
1991 Blackstone River Survey #3
Nitrate in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLX01
1.55
0.24
2.20
0.74
BLK02
1.24
1.88
2.62
1.99
BLK03
1.27
1.01
2.05
2.21
BLK04
1.13
1.30
1.48
2.21
BLK05
0.06
0.07
0.06
0.03
BLX06
1.32
0.95
0.97
1.33
BLX07
0.80
1.42
1.23
1.20
BLK08
1.23
0.90
1.40
1.20
BLK09
0.09
0.10
0.10
0.10
BLK10
ND
0.03
0.04
0.03
BLX11
1.08
0.64
0.80
1.26
BLK12
2.92
0.67
0.63
1.60
BLX13
2.07
0.77
0.68
1.26
BLK14
0.24
0.16
0.15
0.20
BLK15
0.44
0.16
0.18
0.28
BLK16
0.69
0.43
0.42
0.54
BLK17
1.31
0.56
0.56
0.61
BLK18
0.62
1.02
0.71
0.77
BLK19
1.08
0.25
0.54
0.82
BLX20
0.39
0.15
0.51
0.30
BLK21
1.38
0.79
0.54
0.71
ND = Below the detection limit of 0.02 mg/L.
-------
Blackstone River
GRAFTON
WWTP
OUIN.
MILBURY RIVER
WWTP
UBWPAD
n r
WEST
RIVER
NORTH— MUMFORD
BRIDGE R1VER ~
WWTP
WOON.
WWTP
BRANCH
RIVER
3 4
8
1 1
LEGEND:
Survey III, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
12 13 17
18
19 20
21
A
A
h
-i I 1. |
A
¦ i X , -.1 ...I
50
45
40
35
T
$
30
T
A
1
Q A
-i i l—i—
i
4
25
!0
T
A
x
T
A
i
A
T
A
10
RIVER MILES
-------
1991 Blackstone River Survey #1
Orthophosphate in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
0.02
ND
ND
ND
BLK02
0.90
1.00
1.15
0.11
BLK03
0.90
0.86
1.07
0.93
BLX04
0.71
0.76
0.85
0.93
BLK05
0.08
0.03
0.08
0.06
BLK06
0.64
0.43
0.47
0.88
BLK07
0.67
0.57
0.52
0.49
BLX08
0.53
0.48
0.41
0.44
BLK09
0.13
0.02
0.07
0.41
BLX10
0.15
0.02
ND
ND
BLK11
0.38
0.24
0.19
0.10
BLK12
0.46
0.24
0.17
0.10
BLK13
0.31
0.38
0.22
0.12
BLK14
ND
0.05
0.14
ND
BLK15
0.02
0.05
0.08
ND
BLK16
0.03
0.05
NS
ND
BLK17
0.13
0.20
0.05
0.06
BLX13
0.10
0.29
0.17
0.13
BLK19
0.19
0.26
0.19
0.07
BLK20
0.12
0.23
0.18
0.07
BLK21
0.10
0.20
0.11
ND
ND = Below the detection limit of 0.02 mg/L; MS
= Sample missing.
-------
1991 Blaclcstone River Survey #2
Orthophosphate in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLX01
0.05
ND
ND
ND
BLK02
1.20
0.83
1.02
1.34
BLK03
1.04
0.67
1.02
1.46
BLK04
0.81
0.83
0.83
1.40
BLX05
ND
ND
ND
0.06
BLK06
0.65
0.31
0.66
0.90
BLK07
0.32
0.08
0.14
0.58
BLK08
ND
0.04
ND
0.42
BLX09
NO
ND
ND
0.04
BLK10
0.08
ND
ND
0.02
BLK11
ND
ND
ND
0.14
BLK12
0.09
ND
ND
0.17
BLK13
ND
ND
ND
0.12
BLK14
0.06
ND
0.07
0.02
BLK15
ND
ND
0.08
0.02
BLK16
ND
ND
0.06
0.02
BLK17
ND
ND
0.11
0.20
BLK18
1.61
ND
0.18
0.36
BLK19
0.13
ND
0.28
0.33
BLK20
0.10
ND
0.34
0.25
BLK21
0.09
ND
0.28
0.19
ND a Below the detection limit of 0.02 mg/L.
-------
Blcickstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n
0
50
A
WEST
RIVER
NORTH-
BRIDGE
WWTP
MUMFORD
RIVER —
WOON
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey II, August 14-15, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
1
A
A
, i t. . i . ..i_
A
A.
T
A
J.
45
40
35
30
25
12
13
17
18
19 20
21
A
T
A
I
1
i i
1 .
1
A
20
10
. 'i .
5
0
RIVER MILES
-------
1991 Blackstone River Survey #3
Orthophosphate in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLK01
0.05
0.16
0.20
0.07
BLX02
0.34
0.62
0.56
0. 62
BLK03
0.34
0.53
0.49
0.62
3LX04
0.42
0.44
0.42
0.43
BLK05
ND
0.02
0.02
ND
BLK06
0.20
0.26
0.25
0.32
BLK07
0.16
0.26
0.21
0.21
BLK08
0.16
0.21
0.21
0.21
BLK09
ND
0.02
ND
0.10
BLK10
ND
0.02
ND
ND
BLK11
0.21
0.15
0.12
0.15
BLK12
0.25
0.15
0.12
0.15
BLK13
0.16
0.15
0.02
0.15
BLK14
ND
0.03
ND
ND
BLK15
ND
0.03
ND
ND
BLX16
ND
0.03
ND
0.03
BLK17
0.05
0.14
0.05
0.07
BLK18
0.05
0.14
0.14
0.07
BLK19
0.08
0.14
0.11
0.03
BLK20
0.08
0.16
0.08
0.07
BLK21
0.08
0.09
0. OS
0.07
ND =» Below the detection limit of 0.02 ng/L.
-------
Blackstone River
GRAFTON
WWTP
OUIN.
MILBURY RIVER
WWTP
UBWPAO
ZL
WEST
RIVER
3
0
I
A
1
T
A
NORTH- MUMFORD
BRIDGE 51VER
WWTP I
WOON.
WWTP
BRANCH
RIVER
3 4
1 1
12 13 17 18
19 20
LEGEND:
Survey (II, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
f
A
A
A
A
A A
A
50
45
40
35
30
25
20
A
15
A
A
/>
-.1 l— 1 ....
10
0
RIVER MILES
-------
1991 Blackstone River Survey #1
Total Kjeldahl Nitrogen in mg/L
Station
Run #1
Run n
Run #3
Run #4
BLK01
ND
ND
ND
ND
BLX02
1.2
ND
1.7
ND
BLK03
ND
1.1
1-2
ND
BLK04
1.1
ND
ND
ND
BLK05
ND
ND
ND
ND
BLK06
ND
ND
ND
ND
BLK07
ND
ND
ND
ND
BLK08
ND
ND
ND
ND
BLK09
ND
ND
ND
ND
BLK10
ND
ND
ND
ND
BLK11
ND
ND
ND
ND
BLK12
ND
ND
1.2
ND
BLK13
ND
ND
ND
ND
BLK14
ND
ND
ND
ND
BLK15
ND
ND
ND
ND
BLK16
ND
ND
ND
ND
BLK17
ND
ND
ND
ND
BLK18
2.9
1.6
1.7
2.6
BLK19
1.5
1.6
1.6
2.4
BLK20
ND
ND
1.1
ND
BLK21
ND
ND
1.1
1.1
ND * Below the detection limit of 1.0 mg/L.
-------
Blackstorie River
GRAFTON
WWTP
QUIN.
MILBURV RIVER
WWTP-
UBWPAD
11
WEST
RIVER
NORTH- MUMFORD
. 0RIOGE RIVER ~~
WWTP
\IV
WOON.
WWTP
BRANCH
R!VER
G
50
T
A
A
1 2 3 4
1 1
12
LEGEND.
A : Survey I, July 10-11, 1991
— : Point Sources arid River Inputs
1 : Water Quality Stations, 1991
A
45
t
40
I
A
1
35
A
A
T
A
i
30
25
13
Detection Limit
I
A
1
20
RIVER MILES
w
17 18 19 20 21
A
A
A
A A
-
-------
1991 Blackstone River Survey #2
Total Kjeldahl Nitrogen in mg/L
Station
Run #1
Run #2
Run to
Run #4
BLKOI
1.4
ND
ND
ND
BLK02
1.6
ND
ND
ND
BLK03
1.7
ND
ND
ND
BLK04
1.7
1.1
ND
ND
BLK05
ND
ND
ND
ND
BLX06
1.2
ND
ND
ND
BLK07
ND
ND
ND
ND
BLKOS
ND
ND
ND
ND
BLK09
ND
ND
ND
ND
3LK10
ND
ND
ND
ND
BLK11
ND
ND
ND
ND
BLK12
ND
ND
ND
ND
BLX13
ND
ND
ND
ND
BLK14
ND
ND
ND
ND
BLK15
ND
ND
ND
ND
BLK16
NO
NO
ND
ND
BLK17
ND
ND
ND
ND
BLK13
ND
1.2
NO
ND
BLK19
ND
ND
ND
ND
BLX20
ND
ND
ND
ND
BLK21
ND
ND
ND
ND
ND ¦ Belov tiie detection liait of 1.0 mq/L.
-------
Blackstone River
GRAFTON
WWTP
QUIN.
MILBURY RIVER
WWTP-
UBWPAD
JL-
WEST
RIVER
NORTH- MUMFORD
BRIDGE R|VER "
WWTP
tl
WOON.
WWTP
BRANCH
RIVER
3
1 2 3 4
1 1
LEGEND:
A : Survey II, August 14-15,1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
12 13
17
18
19 20
21
A
0
A A
A
A
A
Detection Limit
A
_1 L i.. 1.
T
A
I
T
A
1
i i ¦ i » i i » '
50
45
40
35
30
25
20
A
_u
A
I
A
1
A
J J I I L_—I 1—
15
10
A'
0
RIVER MILES
-------
1991 Blackstone River Survey #3
Total Kjeldahl Nitrogen in mg/L
Station
Run #1
Run #2
Run #3
Run #4
BLX01
HD
ND
ND
ND
BLX02
ND
ND
1.1
ND
BLK03
ND
ND
ND
ND
BLX04
ND
ND
ND
1.0
BLK05
ND
ND
ND
ND
BLK06
ND
ND
ND
ND
BLX07
ND
ND
ND
ND
BLKOS
ND
ND
ND
ND
BLX09
ND
ND
ND
ND
BLK10
ND
ND
ND
ND
BLK11
ND
ND
ND
ND
BLK12
ND
ND
ND
ND
BLK13
ND
ND
ND
ND
BLK14
ND
ND
ND
ND
BLK15
ND
ND
ND
ND
BLK16
ND
ND
ND
ND
BLK17
ND
ND
ND
ND
BLK18
ND
ND
ND
ND
BLK19
ND
ND
ND
ND
BLK20
NS
ND
ND
ND
BLK21
ND
ND
ND
ND
ND = Below the detection limit of 1.0 mg/L; MS ^
Sample missing
-------
Blackstone River
GRAFTON
WWTP
QUIN. -
MILBURY RIVER
WWTP-
UBWPAD
3l_
3
0
1 2
A
50
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~
1 11
WWTP
WOON.
WWTP
BRANCH
RIVER
I
3 4
1 1
12
LEGEND:
A : Survey III. October 2-3, 1991
— : Point Sources and River Inputs
1 : Water Quality Stations, 1991
A A
A
A
A
T
A
I
T
_J I 1 I—
45
40
35
30
25
13
Detection Limit
T
A
1
20
RIVER MILES
v
17 18 19 20 21
A
I
I
A
1
A
A
J > I L
15
10
-------
1991 Slackstcne River Survey 4\
Total (TSS) and Volatile (VSS) Solids
in mg/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TSS
VSS
TSS
VSS
TSS
VSS
TSS
VSS
BLK01
2.4
0.3
2.3
1.8
0.6
ND
3.0
0.8
BLK02
4.6
2.6
3.6
2.0
3.6
3.0
3.8
1.8
BLKQ3
ND
0.6
1.4
0.6
1.8
1.4
ND
ND
BLK04
1.2
ND
1.4
ND
1.4
0,8
1.8
0.8
SLXOS
1.6
1.2
0.6
0.6
ND
ND
0.6
ND
BLKQ6
6.6
2.6
1.3
ND
3.2
1.4
4.0
1.0
BLX07
4.2
2.0
5.2
2.2
9.2
4.3
5.6
2.2
BLK 08
5.0
2.6
6.3
4.0
18.4
6.6
16.2
4.2
BLK09
1.3
1.0
0.6
ND
1.8
0.8
2.6
1.4
BLK 10
2.2
1.2
1.2
0.8
1.2
1.0
2.8
0.8
BLK11
9.4
3.8
6.6
3.4
12.4
5.4
13.4
4.4
BLK12
5.0
2.6
9.4
5.4
7.0
4.0
5.2
1.8
BLK13
6.4
3.2
7.2
4.2
3.4
2.0
6.8
4.6
BLK 14
1.6
1.0
2.0
1.0
0.6
ND
1.4
0.8
BLK15
2.4
1.2
2.6
1.0
i.a
0.8
4.0
1.4
BLK 15
5.4
2.0
5.4
2.2
3.4
1.8
7.0
2.0
BLK17
5.6
2.8
3.2
5.0
7.3
4.6
a.o
3.6
BLK 13
8.6
4.0
2.6
2.0
6.4
2.8
5.4
2.2
BLK19
5.8
2.8
8.0
4.4
8.2
5.0
5.6
2.8
ELK20
3.8
1.3
3.2
2.2
4.3
3.0
3.6
1.2
BLK21
4.6
1.3
5.0
3.0
6. a
3.3
5.6
2.4
ND =« Below the detection limit of 0.5 og/L.
-------
Blcickstone River
GRAFTON
WWTP
30
25
20
15
10
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n
WEST
RIVER
NORTH- MUMFORD
BRIDGE R1VER
WWTP
WOON
WWTP
BRANCH
RIVER
A
1
0
50
A
3 4
1 1
LEGEND:
Survey I, July 10—11,1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
A
A A
45
T
A
1
40
I
12 13
I
A
35
30
25
20
RIVER MILES
17
18
19
20
21
A
1
A J-
T
A
L
A
10
-------
Blackstone River
GRAFTON
WWTP
30
25
20
15
10
0U1N.
MILBURY RIVER
WWTP
UBWPAD
a .
WE5T
RIVER
NORTH- MUMFORO
BRIDGE RIVER
WWTP
WOON
WWTP
BRANCH
RIVER
0
A
3 4
1 1
LEGEND:
Survey I, July 10—11,1991
Point Sources and River Inputs
Water Quality Stations, 1991
s A
, A A
_i 1 1 1 1 L.. ..1 ,L J-
T
A
1
I
T
A
12
13
T
A
i
$
1 ~T
17
18
19 20
?1
T
A
l
£
_i_-L
_J L L-
50
45
40
-55
30
25
_L_
20
T
A
1
A
T
A
X
10
.1- J ..
RIVLR MILLS
-------
1991 Blackstone River Survey #2
Total (TSS) and Volatile (VSS) Solids
in mg/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TSS
VSS
TSS
VSS
TSS
VSS
TSS
VSS
BLK01
3.0
1.6
3.2
1.8
3.4
1.8
4.2
2.0
BLK02
4.8
2.0
3.0
2.6
3.0
2.0
1.4 '
1.2
BLK03
2.0
1.0
1.0
0.6
1.6
1.2
0.6
ND
BLK04
1.8
0.8
2.6
1.2
1.8
1.4
1.8
1.6
BLKOS
1.4
1.0
1.8
0.8
1.6
1.0
NO
ND
BLK06
3.6
1.8
2.8
1.0
1.0
0.6
2.0
1.8
BLK07
8.6
3.2
6.8
3.8
5.6
3.2
4.4
2.4
BLK08
3.8
1.2
6.2
3.6
17.8
4.6
8.6
4.2
BLK09
1.6
1.4
1.6
1.4
1.4
0.8
1.2
ND
BLK10
1.6
0.8
1.4
0.8
1.8
0.6
1.0
0.6
BLK11
5.2
1.8
6.0
3.2
2.4
1.8
4.0
2.2
BLX12
3.6
1.4
5.4
3.4
3.0
2.0
3.2
1.6
BLK13
5.4
3.0
4.4
3.0
4.8
3.4
4.4
2.6
BLK14
2.0
1.4
3.8
2.6
4.0
2.2
2.6
2.0
BLK15
3.8
2.6
5.2
3.2
6.2
4.8
4.0
2.8
BLK16
2.0
1.2
3.2
1.8
5.8
3.0
9.0
3.6
BLX17
3.4
2.2
4.2
1.4
4.4
2.8
4.6
3.2
BLK18
2.4
1.4
5.4
2.8
4.0
3.0
4.2
2.8
BLK19
4.0
2.8
5.8
3.8
6.8
3.8
3.0
1.6
BLX20
2.6
2.2
4.4
1.6
5.4
1.8
7.0
3.6
BLK21
4.8
2.6
7.0
4.2
6.2
5.0
6.8
4.0
ND = Below the detection limit of 0.5 mg/L.
-------
Blacksione River
GRAFTON
WWTP
30
25
20
15
10
QUIN.
MILBURY RIVER
WWTP
UBWPAD
n r
0
A
I
_J i i I
WEST
RIVER
NORTH- MUMPORD
BRIDGE RIVER
WWTP
WOON.
WWTP
BRANCH
RIVER
3 4
1 1
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
T
A
i
A
- I
T
A
1
_l ' » ¦
50
45
40
35
ui
30
12 13 17 18 19 20
21
T
£
A
A
-J 1 J
_i I 4—
25
20
T
A
1
15
T T
f t
T
A
x
i
0
RIVER MILES
-------
Blackstone River
GRAFTON
WWTP
30
25
QUIN.
MILBURY RIVER
WWTP-
UBWPAD
Zl
20
15
10
0
A £
WEST
RIVER
NORTH- MUMFORD
BRIDGE RIVER ~~"
WWTP
WOON.
WWTP
BRANCH
RIVER
1 2 3 4 6
8 1 1
LEGEND:
Survey II, August 14-15, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
I
A
A
¦ » ¦ ' I I I, « I ¦ ' ¦
A
L
£
T A
A
•1.
A A
12 13 17 18 19 20
T
a X
i A
i-
J—i—j—i—t—I—t—t—i—i 1—i i. i i L i .1 i i J i...
T
21
T
£
L t—-i--
50 45 40 35 30 25 20
RIVER MILES
15
10
_J i-.-i.
5
-------
1991 Blackstone River Survey #3
Total (TSS) and Volatile (VSS) Solids
in mg/L
Station
Run
#1
Run
#2
Run
#3
Run
#4
TSS
vss
TSS
VSS
TSS
vss
TSS
vss
BLK01
3.2
1.2
4.0
1.2
4.6
2.8
2.0
ND
BLK02
3.4
2.8
3.6
2.0
5.4
4.0
3.4
2.0
BLK03
4.4
2.6
4.4
2.4
5.6
3.8
3.6
1.6
BLK04
3.3
2.4
3.2
1.0
4.0
3.0
2.4
1.2
BLK05
1.2
1.0
1.0
0.8
0.6
ND
0.6
ND
BLX06
4.2
2.0
5.4
2.6
11.3
5.4
3.6
2.0
BLK07
5.0
2.2
6.2
2.4
3.2
1.6
3.8
1.4
BLX08
7.4
2.8
8.4
3.0
3.0
2.4
5.2
1.8
BLK09
1.6
1.2
1.6
0.8
1.4
1.2
ND
ND
BLX10
1.8
1.4
1.8
1.0
1.2
1.0
1.0
0.8
SLK11
S.4
2.2
6.6
2.4
5.0
1.3
3.6
1.4
BLK12
3.4
1.8
6.6
1.8
2.2
1.4
2.3
1.2
BLK13
4.2
2.0
4.6
1.8
5.4
2.4
2.4
1.0
BLK14
3.0
2.0
3.0
1.2
1.6
1.4
1.0
0.8
BLK15
3.6
2.2
3.0
1.2
2.0
1.4
2.0
1.6
BLK16
3.4
2.0
2.4
0.8
1.0
0.8
2.2
1.4
BLK17
4.0
2.0
5.0
1.6
2.4
1.6
3.8
2.0
BLK18
4.6
2.6
4.0
1.4
3.4
2.2
2.8
2.0
BLX19
3.6
2.2
5.0
2.0
2.2
1.4
2.4
2.0
BLX20
NS
NS
3.6
1.3
2.8
1.6
1.8
1.4
BLK21
4.2
2.0
3 .6
1.2
2.0
1.2
1.4
1.0
ND = Below the detection limit of 0.5 mg/L; NS » Sample
missing.
-------
Blackstone River
GRAFTON
WWTP
30
25
20
15
10
QUIN.
MILBURY RIVER
WWTP-
UBWPAD
ZL_
WEST
RIVER
NORTH- MUMFORD
BRIDGE r,v/ER —"
WWTP
WOON
WWTP
BRANCH
RIVER
0
T
A
1
50
£
3 4
1 1
LEGEND:
Survey III, October 2 — 3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
A
~ T
A
T
A
I
A
i
J.
T
A
1
45
40
35
30
12
T
A
1
25
13
T
A
1
20
RIVER MILES
17 18 19 20 21
T
A
1
A
15
T
t 4
10
1 ¦
A
1
_.j..
5
-------
Blackstone River
GRAFTON
WW VP
QUIN. -
MJLBURY RIVER
WW1P-
UBWPAO
50
n
rT~rrr
1 2 3 4 6
WEST
RIVER
NORTH— MUMfORD
BRIDGE _RIVER ~
WW TP
WOON-
VWVTP
BRANCH
RIVER
1 \
LEGEND:
Survey lit, October 2-3, 1991
Point Sources and River Inputs
Water Quality Stations, 1991
T
£
« ' i 1
$ T
£
45
1
40
I
A
A
-a—i—a—u
35
30
I
25
12 13
17
18
i £
A
20
T
£
~X 1 1
15
19 20
21
A
A
A
10
. I L
RIVER MILES
-------
1991 Blackstone River
Total (TTM} and Dissolved (DTM} Trace Metals
WoonsocXet Wastewater Secondary Effluent Pre-Chlorination
Parameter
Survey
~ay 1
Day 2
Day 3
Day 4
Day 5
Cadmium
1
3.1
2.1
2.4
2.4
2.4
TTM
2
4.8
3.5
3.8
4.5
11.6
(M9/L)
3B
0.9
1.5
1.9
Cadmium
1
2.2
1.7
1.4
1.5
1.9
DTM
2
4.3
3.0
1.6
3.7
6.5
(w/U
30
0.3
0.6
0.7
Chromium
1
3.9
4-5
2.7
5.1
7.2
TTM
2
9.0
9.1
4.8
5.1
6.0
O^g/D
3B
0.9
2.7
2.7
chromium
1
1.6
3.4
ND
2.4
4.6
DTM
2
7.3
5.5
1.6
4.6
4.9
(M9/I>)
3B
0.4
1.9
1.9
Copper
1
41.1
33.3
54.1
44.1
32.1
TTM
2
24.6
41.4
36.9
37.3
54.0
(M/L)
3B
23.2
24.6
21.6
Copper
1
27.1
25.0
31.0
36.5
22.5
DTM
2
13.0
28.0
12.7
32.3
51.1
(jtg/i»)
3B
5,9
13.0
12.1
Lead
1
5.2
3.6
5.1
5.7
3.9
TTM
2
12.3
10.1
6,9
9.6
13.9
(M9/L)
3B
7.2
5.1
6.9
Lead
1
2.4
1.6
1.6
1.6
1.9
DTM
2
4.7
5.5
ND
7.9
14.5
(M9/L)
3B
1.9
2.2
1.0
Nickel
1
ia. 5
10.3
13.0
9.0
13.8
TTM
2
9.3
98.7
7.1
9.9
11.4
(M9/L)
3B
13.9
17.1
10.4
Nickel
1
6.8
16.8
8.6
5.7
3.4
DTM
2
14,4
8.1
6.9
6.9
9.3
(jug/t)
3B
16.8
a.4
9.9
-------
1991 Blackstone River
Total (TTM) and Dissolved (DTM) Trace Metals
Woonsocket Wastewater Secondary Effluent Post-Chlorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
Cadmium
1
3.2
2.7
2.6
3.0
2.7
TTM
2
4.8
4.8
3.3
5.2
5.9
(M9/L)
3B
ND
1.1
1.7
Cadmium
1
2.2
3.1
1.6
1.9
1.9
DTM
2
4.5
3.5
2.9
2.5
4.4
(M9/L)
3B
0.3
0.8
0.7
Chromium
1
4.2
5.4
3.6
3.9
3.9
TTM
2
14.7
9.0
5.7
0.9
4.8
(Mg/L)
3B
ND
2.1
3.3
Chromium
1
3.1
1.3
0.4
1.6
3.7
DTM
2
9.1
5.5
3.1
2.2
3.1
(Mg/L)
3B
ND
1.0
1.9
Copper
1
38.4
46.2
36.9
39.0
44.4
TTM
2
33.0
43.5
39.0
147.0
39.9
(Mg/L)
3B
7.5
9.0
20.0
Copper
1
25.6
32.2
22.0
18.4
30.1
DTM
2
30.1
35.5
31.5
29.5
29.1
(Mg/L)
3B
3.4
10.9
9.4
Lead
1
3.9
5.1
3.9
6.3
4.2
TTM
2
10.8
8.7
9.6
21.0
12.3
(Mg/L)
3B
3.0
3.9
6.0
Lead
1
1.6
1.6
0.7
1.6
1.3
DTM
2
5.2
5.2
2.5
4.3
6.7
(Mg/L)
3B
1.0
2.8
2.2
Nickel
1
205
223
256
263
121
TTM
2
168
196
222
255
210
(Mg/L)
3B
16
69
79
Nickel
1
192
207
. 223
238
120
DTM
2
164
160
205
207
187
(Mg/L)
3B
10
71
11
-------
1991 Blackstone River Canventionals Parameters
Upper Blackstone (TJBPAD) Wastewater Secondary Effluent
Pre-Chlorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
CacLnium
1
4.5
4.0
3.6
4.5
5.5
TTM
2
1.2
4.9
4.5
4.9
5.0
(Mg/L)
33
3.0
3.0
3.4
Cadmium
1
4.0
1.9
3.0
3.8
5.8
DTM
2
1.9
4.3
4.3
4.7
4.2
(Mg/L)
3B
3.0
2.8
2.6
Chromium
1
5.1
3.3
4.8
6.9
4.5
TTM
2
3.7
3.6
52.8
3.0
3.3
(Mg/L)
3B
1.2
1.2
1.8
Chromium
1
4.0
0.7
2.2
5.8
4.3
DTM
2
3.5
2.8
3.7
2.2
2.5
(Mg/L)
3B
0.7
0.7
1.3
Capper
1
36.3
36.6
40.2
42.6
43 .8
TTM
2
23.2
43.5
18.6
20.1
12.6
(Mg/L)
3B
21.0
30.9
29.4
Copper
1
28.3
33.1
35.2
35.5
36.1
DTM
2
26.5
25.0
19.1
13.9
3.9
(Mg/L)
3B
14.5
25.2
19.9
Lead
1
6.9
7.4
5.7
6.5
6.6
TTM
2
6.1
4.3
5.1
3.2
3.8
(Mg/L)
33
1.5
1.8
1.8
Lead
1
3.7
4.6
3.7
4.1
3.1
DTM
2
5.9
2.3
3.6
1.4
1.9
(Mg/L)
3B
0.4
1.0
0.7
Nickel
1
22.2
18.6
15.6
14.7
32.1
TTM
2
122
79.5
67.5
58.2
44.7
(Mg/L)
33
18.0
20.4
27.3
Nickel
1
22.2
13.2
15.6
15.0
33.3
DTM
2
120
75.3
65.7
55.8
39.9
(Mg/L)
3B
15.3
21.0
24.0
-------
1991 Blackstone River Conventional Parameters
Upper BlacJcstone (UBPAD) Wastewater Secondary Effluent
Post-Chlorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
Cadmium
1
4.6
4.4
4.0
4.1
4.2
TTM
2
2.1
6.8
4.5
4.5
5.2
(Mg/L)
3B
3.2
2.4
2.5
Cadmium
1
3.5
2.8
3.2
3 .1
3.8
DTM
2
1.9
4.7
3.5
3.7
4.9
(Mg/L)
3B
2.5
1.7
2.1
Chromium
1
3.9
6.6
6.0
7.2
7.5
TTM
2
5.7
5.1
3.6
6.9
4.5
(Mg/L)
3B
2.1
1.5
2.7
Chromium
1
1.6
3.4
2.2
2.8
5.5
DTM
2
4.0
2.5
1.3
1.3
3.1
(Mg/L)
38
0.7
ND
1.3
Copper
1
45.6
43.5
59.4
45.0
61.1
TTM
2
44 .7
33.0
24.6
27.6
26.4
(Mg/L)
3B
21.6
25.5
41.4
Copper
1
33.1
26.8
31.9
32.5
43.3
DTM
2
46.0
15.4
11.5
19.3
147
(Mg/L)
3B
14.8
21.4
32.1
Lead
1
5.4
5.9
6.2
6.8
6.9
TTM
2
8.6
3.9
4.6
4.4
3.2
(Mg/L)
3B
2.1
1.8
2.4
Lead
1
3.3
4.5
4.2
3.9
4.2
DTM
2
8.1
1.9
3.2
1.6
1.5
(Mg/L)
3B
0.3
2.4
0.9
Nickel
1
27.6
24.0
21.8
21.0
23.7
TTM
2
163
94. a
76.8
66.0
52.2
(Mg/L)
3B
17.1
23.2
25.8
Nickel
1
24.0
16.5
17.4
14.7
20.4
DTM
2
150
87.0
69.8
60.0
49.5
(Mg/L)
3B
15.9
19.8
21.6
-------
1991 Blackstane River
Woonsocket Wastewater Secondary Effluent Pre-Chlorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
TSS
1
7.2
10.4
5.0
11.4
3.8
(mg/L)
2
16.4
14.6
3.3
7.6
29.4
3A
£.0
8.4
22.2
33.2
28.4
3B
28.6
28.8
38.2
VSS
1
7.8
3.0
3.2
3.8
6.4
(mg/L)
2
10.8
10.4
6.4
5.6
19.2
3A
6.4
7.0
16.3
25.6
23.6
3B
20.3
21.6
27.7
Calcium
1
11.0
13.0
12.0
13.0
13.0
(mg/L)
2
12.7
13.3
12.0
14.3
13.7
38
13 .6
14.3
13.8
Magnesium
1
4.2
4.3
4.1
3.9
3.5
(mg/L)
2
3.8
3.7
3.8
3.9
3.3
3S
4.0
3.9
4.6
B0D5
1
3.1
5.0
6.9
6.5
9.9
(mg/L)
2
9.2
7.2
19.1
10.2
10.5
3A
13.4
10.2
13 i 6
25.5
21.9
3B
20.8
22.0
22.0
Chloride
1
125
105
85
247
335
(ag/L)
2
225
200
145
160
263
3A
258
212
202
240
410
38
330
323
159
Ammonia
1
26.6
33.8
28.2
21.5
21.4
(mg/L)
2
IS,2
15.0
13.5
12*0
13.2
3A
6.5
10.3
13.0
13.0
6.1
38
12.1
10.5
8.9
P04
1
3.6
2.6
3.5
3.1
1.3
(mg/LJ
2
3.5
3.6
4.7
4.6
4.7
3B
4.2
4-6
3.9
Nitrate
1
0.4
0.4
0.4
0.3
0.3
(mg/L)
2
1.7
3.7
29.7
33.2
39.6
3B
1.6
2.1
3.8
TKN
1
32.1
27.2
38.8
NS
24.3
(mg/L)
2
13.6
15,4
19.5
15.6
17.6
3A
9.3
a.7
7.7
3B
15.1
13.1
11.1
MS » Sample missing or insufficient volume.
-------
1991 Blackstone River
Woonsocket Wastewater Secondary Effluent Post-Chlorination
Parameter
Survey
Day l
Day 2
Day 3
Day 4
Day 5
TSS
1
6.0
8.2
7.6
NS
7.0
(mg/L)
2
14.6
16.6
8.4
8.8
22.8
3A
16.9
16.0
15.0
20.4
24.4
3B
13.2
23.6
26.4
VSS
1
4.8
6.2
7.2
NS
5.2
(mg/L)
2
10.8
12.2
5.2
4.8
15.2
3A
14.0
12.9
13.3
17.6
19.2
3B
9.6
17.6
20. 0
Calcium
1
11.0
12.0
13.0
11.0
12.0
(mg/L)
2
11.8
13.4
13.1
13.8
12.9
3B
12.9
14.1
13.4
Magnesium
1
4.0
4.3
4.3
4.7
3.4
(mg/L)
2
3.3
3.6
3.9
3.8
3.7
3B
3.9
4.1
4.8
B005
1
4.2
7.5
3.7
NS
7.6
(mg/L)
2
8.4
7.5
14.4
12.2
6.2
3A
11.9
8.7
7.7
13.5
18.5
3B
14.0
20.0
16.0
Chloride
1
154
114
100
NS
142
(mg/L)
2
290
283
200
175
205
3A
310
260
232
273
440
3B
335
210
191
Ammonia
1
28.6
27.7
27.8
28.4
NS
(mg/L)
2
12.9
9.5
12.0
16.8
14.3
3A
7.7
9.9
15.8
13.8
8.4
3B
13.2
13.5
10.5
P04
1
3.9
3.0
3.5
NS
3.1
(mg/L)
2
3.7
3.7
4.5
ND
4.9
3B
4.1
4.2
3.7
Nitrate
1
0.9
0.9
0.9
NS
1.0
(mg/L)
2
3.8
2.0
38.8
9.9
58.5
3B
1.4
2.7
4.7
TXN
1
25.6
13.4
42.0
NS
42.0
(mg/L)
2
13.7
17.1
18.8
17.2
17.8
3A
6.4
7.5
7.5
3B
11.5
13.5
13 .7
MS * Sample missing or insufficient volume.
-------
1991 Blackstone River
Upper Blackstone (UBPAO) Wastewater
Secondary Effluent Pre-Ciilorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
TSS
1
2.4
1.3
2.4
2.0
1.8
(mg/L)
2
1.4
4.4
1.3
0.6
3.2
3A
4.4
5.2
3.6
4.6
7.6
3B
2.0
1.2
2.4
vss
1
2.0
0.8
2.2
1.6
1.0
(mg/L)
2
0.3
3.2
1.0
ND
2.6
3A
3.2
2.8
3.0
4.0
6.0
3B
l.S
0.8
1.8
Calcium
1
13.0
13.0
12.0
12.5
13.0
(mg/L)
2
12.3
12.3
13.2
11.9
13.2
38
13.3
12.7
13.4
Magnesium
1
3.2
3.1
3.0
3.0
3.1
(mg/L)
2
3.3
3.1
3.2
3.1
3.2
3B
3.4
3.1
3.2
BODS
1
3.0
1.8
1.0
3.0
5.0
(mg/L)
2
2.0
3.5
2.3
1.9
ND
3A
6.7
3.7
3.4
2.9
3.5
3B
5.2
5.5
3.2
Chloride
1
92
92
78
85
85
(mg/L)
2
74
50
69
103
105
3A
65
73
95
96
102
3B
120
115
130
Ammonia
1
0.8
1.0
0.2
0.5
0.2
(ng/L)
2
0.2
0.3
0.2
0.3
0.3
3A
0.5
0.4
0.2
1.0
0.4
3B
0.3
0.4
0.2
P04
1
2.3
2.0
NS
2.5
1.4
(ng/L)
2
2.0
2.9
2.1
2.1
2.9
2B
3.1
2.5
2.6
Nitrate
1
6.3
5.7
MS
6. 4
6.3
(mg/L)
2
7.2
3.4
26.8
12.3
23.6
3B
13.0
7.7
10.4
TKN
1
2.0
2.2
2.9
1.0
1.0
(mg/L)
2
1.4
1.1
1.1
1.3
1.3
3A
ND
ND
ND
3B
ND
ND
ND
MS a Sample missing or insufficient volume.
-------
1991 Blackstone River
Upper Blackstone (UBPAD) Wastewater
Secondary Effluent Post-Chlorination
Parameter
Survey
Day 1
Day 2
Day 3
Day 4
Day 5
TSS
1
8.6
4.3
5.0
NS
NS
(mg/L)
2
6.0
2.4
2.4
2.2
20.4
3A
3.0
2.3
5.8
NS
4.6
3B
2.3
3.6
3.0
vss
1
7.0
4.0
3.8
NS
NS
(mg/L)
2
4.4
1.6
2.0
1.2
18.4
3A
6.3
2.4
4.6
NS
3.8
3B
1.8
3.2
2.2
Calcium
1
13.0
13 .0
13 .0
12.0
13.0
(mg/L)
2
11.8
13.1
13.6
11.9
13 .2
35
12.7
12.4
13.1
Magnesium
1
3.2
3.2
3.0
3.0
3.1
(mg/L)
2
3.4
3.2
3.2
3.3
3.3
3B
3.5
3.4
3.5
BOOS
1
5.0
3.3
2.0
2.8
5.0
(mg/L)
2
3.8
4.6
5.7
3.9
NS
3A
2.9
1.7
3.2
1.9
2.5
3B
4.5
3.2
4.7
Chloride
1
92
96
92
88
88
(mg/L)
2
89
62
68
98
132
3A
73
92
111
101
111
3B
123
125
141
Ammonia
1
1.1
0.6
0.1
0.2
0.2
(mg/L)
2
0.2
0.6
0.1
0.1
NS
3A
0.8
0.2
0.2
0.4
0.4
3B
0.2
0.1
0.2
P04
1
2.5
2.0
2.4
3.0
1.6
(mg/L)
2
2.1
3.0
1.9
2.2
2.6
3B
3.3
2.4
3.4
Nitrate
1
6.8
5.7
6.4
7.1
6.4
(mg/L)
2
6.0
26.3
30.9
8.8
31. 6
3B
13.4
9.8
11.8
TKN
1
2.5
2.2
1.6
1.1
1.5
(mg/L)
2
2.3
1.8
1.4
1.4
1.3
3A
ND
ND
ND
3B
ND
ND
1.2
NS = Sample missing or insufficient volume.
-------
Appendix C
1991BLACKSTONE RIVER SURVEY
QUALITY ASSURANCE/QUALITY CONTROL
-------
MEMORANDUM
Blackstone River 1990 Study
Civil and Environmental Engineering
University of Rhode Island
June 1, 1992
The laboratory procedure used for the analyses of the
Blackstone River 1990 survey are in accordance with the
Work/Quality Assurance Project Plan prepared for the U. s.
Environmental Protection Agency Region I by the civil and
Environmental Engineering Department (CVE) in July 1991. In
addition to this document other provisions were required as
outlined in the August 2, 1991 memorandum from carol M. Wood,
Quality Assurance officer for EPA to Katrina Kipp, EPA Project
Officer. These provisions were handled in the following manner:
1. The preventive maintenance schedule was expanded to
include routine daily and weekly maintenance practices.
2. All samples containing chloroform as a preservative have
been retained for disposal in a manner appropriate for wastes
containing carcinogens.
3. a comparison was completed for the second round sampling
for ammonia analyses of the two effluent stations. Samples were
split and. analyzed both with and without a distillation step. A
copy of those results are attached as Table 1. The results do
not appear to be dissimilar. Distillation did not occur for
survey 3 ammonia samples.
4. A comparison was completed for the second round sampling
for the trace metal analyses of the two effluent stations.
Samples were analyzed using the method of acid extraction as
well as the method of acid digestion. A copy of those results
are attached as Table 2. The results do not appear to be
dissimilar. Digestion did not occur for survey 3 metal
analyses.
In addition to these provisions a summary of the accuracy,
precision and completeness of the data are given in Table 3.
Accuracy is represented by the percent mean recovery and
precision is represented by the standard error of the mean. The
results are in accordance with the expectations given in the QA
Project Plan. Accuracy of the BOD test was determined by
running five duplicate synthetic water samples containing a 1:1
mixture of glucose and glutamic acid. These data are given in
Table 4. The results are in accordance with the expectations
given in the QA Project Plan. Trip blanks are summarized in
Table 5 and indicate no significant contamination.
-------
UOl Ui( 13J4 JCJ 4Ui" / oz-iiaoo
uki uut utMiNS urrxui
r«at
-------
Table 2
Comparison Between Total Trace Metal Results of Analyses Using
Acid Extraction (AE) and Acid Digestion (AD) Methods
Blackstone River 1990 Survey 2
Woonsocket and UBPAD Effluents
Station
Chromium
Cadmium
Copper
Lead
Nickel
AE
AD
E-D
AE
AD
E-D
AE
AD
E-D
AE
AD
E-D
AE
AD
E-D
UBPAD Pre-Cl 1
5.7
5. 3
0.4
2.1
2.4
-0. 3
44.7
46.7
-2.0
8.6
7.5
1.1
163
150
13
UBPAD Pre-Cl 2
5.1
4.7
0.4
6.8
6.3
0.5
33.0
38.9
-5.9
3.9
4.1
-0.2
94.8
89.6
5.2
UBPAD Pre-Cl 3
3.6
3.9
-0.3
4.5
3.9
0.6
24.6
27.3
-2.7
4.6
4.2
0.4
76.8
82.9
- 6.1
UBPAD Pre-Cl 4
6.9
5.9
1.0
4.5
5.0
-0.5
27.6
33.2
-5.6
4.4
4.0
0.4
66.2
67.8
- 1.6
UBPAD Pre-Cl 5
4.5
4.1
0.4
5.2
4.8
0.4
26.4
30.4
-4.0
3.2
2.8
0.4
52.2
55.5
- 3.3
Averages
5.2
4.8
4.7
4.4
31.3
35.3
4.9
4.6
90.6
89.2
WOON Pre-Cl 1
14.7
13.8
0.9
4.8
4.4
0.4
33.0
29.8
3.2
10.8
9.7
1.1
168
159
9
WOON Pre-Cl 2
9.0
8.4
0.6
4.8
4.9
-0.1
43.5
39.3
4.2
8.7
8.8
-0.1
196
180
16
WOON Pre-Cl 3
5.7
5.3
0.4
3.3
3.9
-0.6
39.0
43.2
-4.2
9.6
8.1
1.5
222
204
18
WOON Pre-Cl 4
0.9
1.3
-0.4
5.2
4.9
0.3
45.2
41.9
3.3
21.0
20.6
0.4
255
224
31
WOON Pre-Cl 5
4.8
4.4
0.4
5.9
6.2
-0.3
39.9
44.2
-4.3
12.3
12.8
-0.5
211
203
8
Averages
7.1
6.6
4.8
4.9
40.1
39.7
12.5
12.0
210
194
-------
(jd/u^/133^ zti:ou tai-/a^-iUbb
urci out Li&Mho urriot
rwt oo
Table 3
summary of Results for the Quality Assurance Project Plan
Parameter
Accuracy
Precision
Completeness
Percent Mean
Mean Standard
Percent
Recovery
Error
Ammonia
95.6
0.0
99
B°d5
—
0.1
100
Calcium
101.5
0.2
99
Chloride
98.2
1.1
99
Chlorophyll a
-
0.7
100
Magnesium
100.5
0.0
100
Nitrate+Nitrite
96.3
0.1
99
Orthophosphate
92.4
0.0
99
TKN
—
0.0
99
Total Cadmium
97.8
0.04
100
Total Chromium
100.1
0.09
100
Total Copper
103.3
0.27
100
Total Lead
95.6
0.32
100
Total Nickel
101.3
0.28
100
-------
TABLE 4
Accuracy Analyses of the Conventional Parameters in Blackstone Study
BODj GLUTAMIC TEST
STM TOheoretical
Reference of BODs
(mg/L)
Pre-Survey II
Measured BOD5
(mg/L)
Pre-Survey IH
Measured BODs
(mg/L)
2.0
1.8
1.6
5.9
5.9
4.3
14.9
14.1
13.5
19.8
20.5
18.0
198.0
192.6
188.0
NOTES:
Dissolved Oxygen Probe was calibrated by Winkler Method before
each Glutamic Test
STM Reference method is 5210 B,
-------
UKi OUC. U04IN9 UrriOL
rwa c. a *
TABLE 5
Trip Blanks Analyses of the Measured Parameters in Blackstone Study
Parameter
Survey No. I
Survey No. II
Survey No. Ill
Ammonia, mg/L
NS
ND
ND
BODs
NS
ND
ND
Calcium, mg/L
ND
ND
ND
Chloride, mg/L
NS
ND
ND
Magnesium, mg/L
ND
ND
ND
Nitrate+Nitrite, mg/L
NS
ND
ND
Orthophosphate, mg/L
NS
ND
ND
TKN, mg/L
NS
ND
ND
Suspended Solids, (mg/L)
TSS
NS
ND
ND
VSS
NS
ND
ND
Trace Metals, (ug/L)
Cadmium
0.04
0.03
0.03
Chromium
0.3
0.3
0.2
Copper
0.5
0.5
0.4
Lead
0.5
0.1
0.2
Nickel
0.2
0.3
0.1
NOTES:
NS = No Sample or insufficient volume.
Represent averages of trip blank samples collected in a survey.
-------
Appendix D
1991 BLACKSTONE RIVER SURVEY
BIO METHODS
-------
APPENDIX D
WHOLE SEDIMENT TOXICITY TESTS USING Hvallela azteca
Age of Organism: Start a test with 2nd or 3rd instar; 2-3 mm in length. To
obtain amphipods, leaf material from the cultures are placed on 5-10 mm
mesh sieve. The sieve is placed over a collecting pan containing 2 cm culture
water. To separate the juvenile amphipods from the larger adults, a stack of
sieves with the sizes #30, #40, and #60 is used. The cultures are siphoned
onto the stack of sieves which are sitting in a plastic tray with culture water
covering the mesh of the bottom sieve. The water and organisms can then be
siphoned from the bottom sieve, a standard #60, into a crystallizing dish. The
dishes are examined on a light table. The Hvallela are removed using a
disposable pipet and counted. They are put into a beaker of culture water,
aerated and feed lightly. Keep in the dark until the test starts. They can be
held for 24 hours before the test.
Test Vessels: Use four replicate 1-L beakers per treatment with 20 H. azteca
per replicate.
Acclimation: If amphipods are cultured in water different from the
overlyin/g water, acclimation is necessary. Place animals in a 50:50 mixture
of culture water : overlying water for 2 hours. Then for 2 more hours put
them in a 25:75 mixture followed by a transfer to 100% overlying water. At
this stage the amphipods are considered acclimated to the overlying water
and are ready for immediate use. Select organisms randomly with a pipette
and place in 30 ml counting beakers that can be floated in the test chambers
before the amphipods are introduced into the sample.
Test duration:
-------
trapped in the surface tension of the water. If floaters are detected, they are
replaced with new animals.
Feedings: Ground rabbit pellets dispersed in deionized water. Mix with
magnetic stirrer to homogenize food. If dissolved oxygen drops to 40%
saturation, suspend feeding. If fungus or bacteria grow on the surface
sediment, suspend feeding also for a day or two. Feed 6 mg. rabbit pellets per
feeding per 20 amphipods in a 1 liter beaker three times weekly for the first
week. Increase to 12 mg per feeding during the second week.
Endpoints and Observations:
Make notes of behavioral changes during the conduct of the test (floating on
surface, sediment avoidance). At the end of the test, the sediment is screened
using a #35 and a # 50 US standard size sieve.
Live and dead amphipods are recorded. At the end of round 3 Hvallela
testing, the sieved sediments were preserved with ethanol and sealed for later
examination and counting. The recovered Hvallela were counted as live at
the end of the test, because they tend to readily decompose after dying during
a test.
WHOLE SEDIMENT TOXICITY TESTS USING Chironomus tentans
Age of Organism: A test should be started with 2nd instar larvae. Egg cases
are obtained by aspirating adults into a 1000 ml Erlenmeyer flask in the
morning. In late afternoon, 300 ml. of culture water is added to the flask. Egg
cases are deposited overnight and 1st instar larvae begin to hatch after about 3
days at 20 degrees C. 12-16 days before a test is begun, 3 freshly laid midge cases
should be placed in a large flask or enameled rearing pan with water to a
depth of 3 cm. No substrate is added to the pan. Fish food flakes (Tetramin)
should be added at a rate of 3 ml/day (10 g/100 ml. blended in water). Fresh
water should be added as necessary to make up for evaporation. The larvae
in the flasks/pans are presumed to be 2nd instars on the 12th day from the
time the eggs were laid. Most larvae will remain as 2nd instars until day 16.
Larvae > 16 days old should not be used to start a test. Start a rearing pan
every 4 days for ongoing toxicity testing.
Handling: Midges should be handled as little as possible. Larvae should be
transferred with a 7 mm glass pipet. Midges should be introduced into
solutions beneath the air-water interface. Any midges that touch dry surfaces,
are dropped or injured during handling should be discarded.
Acclimation: If the midges are cultured in water different from the overlying
water or temperature, an acclimation process is necessary. Place animals for 2
hours in a 50:50 mixture of culture water to overlying water, then for 2 hours
in a 25:75 mixture of culture water to overlying water, followed by a transfer
into 100% overlying water. Midges should be randomly selected from the
-------
acclimation water with a pipette and placed into counting beakers ie. 30 ml
beakers that can be floated in the test chambers before the midges are
introduced into the exposure system.
Test design: Use 2 liter beakers containing 2 cm of sediment and 1.5 L of
overlying water in static testing. Add 20 C. tentans per beaker.
Test Initiation: Sediments should be homogenized and placed in the test
chambers on the day before addition of test organisms (Day -1). Test chambers
should be covered and overlying water aerated overnight. The test begins
when the midges are introduced to the
test chambers. Test chambers should be inspected < 2 hours after midges are
introduced to insure that animals are not trapped in the surface tension of the
water. These floaters do not survive well and should be replaced with
healthy animals.
Feeding: Feed 50 mg. fish food flakes (dry weight, administered in a 0.5 ml
suspension) daily to each beaker. If food collects on sediment, fungus or
bacteria may grow and feeding should be suspended for a day or more. A
drop in dissolved oxygen to 40% saturation might indicate that all of
the food is not being consumed so feeding should also be suspended.
Endpoints : Larval survival and growth can be assessed by ending the tests
between days 10 to day 14 when larvae have reached the 3rd or 4th instar. At
this time, the larvae can be removed from sediment using a #35 sieve. The
midges can be rinsed from the sieve into collecting pans and pipeted
from the rinse water. Growth determinations using dry weight (dried at 60
degrees C) are preferable to length.
-------
Appendix E
1991BLACKSTONE RIVER SURVEY
BIO TAXA
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BIOl (River Mile 44.0)
DWPC Station # BS10
TOLERANCE
VALUE*
ROCK BASKETS
KICK NETS
ANNELIDA
Oligochaeta (Aquatic Earthworms)
Tubificida
Tubificidae 10
Hirudinea (Leeches)
Rhynchobdellida
Glossiphoniidae
Helobdella sp. 7
Pharyngobdellida
Erpobdellidae
Erpobdella sp. 8
ARTHROPODA
Crustacea
Isopoda
Asellidae
Caecidotea sp. 8
Amphipoda
Gammaridae
Gammarus sp. 6
Talitridae
Hvalella sp. 8
Insecta
Odonata
Anisoptera (Dragonflies)
Aeschnidae
Boveria sp. 2
Zygoptera (Damselflies)
Coenagrionidae
Araia sp. 6
Enallaama sp. 8
Hydropsychidae
Cheumatoosvche sp. 5
12 2
15 4
2 2
3 5
13 5
E-l
-------
APPENDIX E
BEHTHIC MACROINVERTEBRATE DATA (Continued)
EPA Station # BIOl (River Mile 44.0)
DWPC Station # BS10
TOLERANCE
VALUE*
ROCK BASKETS
A B C
KICK NETS
Elmidae (Riffle Beetles)
Microcvlloepus sp.
Diptera (True Flies)
Tipulidae (Crane Flies)
Tipula sp.
ceratopogonidae (Biting Midges)
Bezzia sp.
Simuliidae (Black Flies)
Simulium sp.
Chironomidae (Midges)
Tanypodinae
Clinotanvoua sp.
Conchapelopia sp.
Thienemannimvia sp.
Orthocladiinae >
Cricotopus sp.
Orthoeladius sp.
chironominae
Chironomus sp.
Glvptotendipes sp.
Polvpedilum sp.
Rheotanvtarsus sp.
Empididae
Hemerodromia sp.
MOLLUSCA
Gastropoda (snails)
Mesogastropoda
Basomatophora
Physidae
Phvsella sp.
4
6
4.5
8
1
1
6
16
19
16
8
10
14
6
1
3
7
2
3
1
1
6
1
10
1
3
2
10
1
6
72
55
60
52
65
73
6
1
6
3
3
5
1
3
3
Total # of Individuals
100 100 100
93 96
103
•Tolerance Value: From Bode, et al. , 1991; for taxa not listed in Bode,
tolerance values used were taken from DWPC, 1990.
E-2
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BX02 (River Mile 39.7)
DWPC Station < BS12
TOLERANCE
VALUE
ROCK BASKETS
ABC
KICK NETS
ABC
ANNELIDA
Oligochaeta (Aquatic Earthworms)
Lumbricina
Tubificida
Tubificidae
Limnodrilus sp.
Hirudinea (Leeches)
Rhynchobdellida
Glossiphoniidae
Helobdella sp.
Pharyngobdellida
Erpobdellidae
Eroobdella sp.
ARTHROPODA
Crustacea
Isopoda
Asellidae
Caecidotea sp.
Amphipoda
Gammaridae
Gammarus sp.
Insecta
Ephemeroptera (Mayflies)
Baetidae
Baetis sp.
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp.
10
12 1
3 2 3
2 13
12 1
6 12 9
9 5 6
E- 3
-------
APPENDIX E
BENTHIC MACROINVERTEBRATE DATA (Continued)
EPA station # BI02 (River Mile 39.7)
DWPC Station # BS12
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatopsvche sp.
Hvdropsvche sp.
Diptera (True Flies)
Tipulidae (Crane Flies)
Tioula sp.
simuliidae (Black Flies)
Simulium sp.
Prosimulium sp.
Chironomidae (Midges)
Tanypodinae
Ablabesmvia sp.
Conehapelopia sp.
orthocladiinae
Cricotopus sp.
Nanoeladius sp.
Orthocladius sp.
Rheoericotopus sp.
Chironominae
Polvoedilum sp.
5
4
4.5
2
8
6
3.5
7
3
6
6
6 10 4
76 78 86
14 7 9
44 48 41
13
MOLLUSCA
Gastropoda (Snails)
Mesogastropoda
Basomatophora
Physidae
Phvsella sp.
Total # of Individuals
100 100 100 95 101 98
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station BI03 (River Mile 33.6)
DWPC Station # BS14
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
ANNELIDA
Hirudinea (Leeches)
Pharyngobdellida
Erpobdellidae
Erpobdella sp.
ARTHROPODA
Crustacea
Isopoda
Asellidae
Caecidotea sp.
Insecta
Ephemeroptera (Mayflies)
Baetidae
Baetis sp.
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatopsvche sp.
Hydropsvche sp.
Diptera (True Flies
Tipulidke (Crane Flies)
Tipula sp.
Chironomidae (Midges)
Tanypodinae
Conchaoelopia sp.
Orthocladiinae
Cardiocladius sp.
Cricotopus sp.
Nanocladius sp.
Orthocladius sp.
Paracricotopus sp.
Chironominae
Polvpedilum sp.
Rheotanvtarsus sp.
Empididae
Hemerodromia sp.
30
64
27
62
30
62
23
72
26
62
2
1
28
65
Total # of Individuals
100 100 100
108 103 107
E-5
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BI04 (River Mile 27.4)
DWPC Station # BS16
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
ANNELIDA
Hirudinea (Leeches)
Pharyngobdellida
Erpobdellidae
Erpobdella sp.
AKTHROPODA
Crustacea
Amphipoda
Ganmaridae
Gammarus sp.
Insecta
Ephemeroptera (Mayflies)
Baetidae
Acentrella sp.
Megaloptera
corydalidae (Dobsonflies)
Niaronia sp.
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatoosvche sp.
Hvdropsvche sp.
Diptera (True Flies
Tipulidae (Crane Flies)
Tipula sp.
Simuliidae (Black Flies)
Simulium sp.
Chironomidae (Midges)
Tanypodinae
Conchapelopia sp.
orthocladiinae
Cardiocladius sp.
Cricotoaus sp.
Orthocladius sp.
chironominae
Polvpedilum sp.
Rheotanvtarsus sp.
Tanvtarsus sp.
Total # of Individuals
5
4
4
4.5
57
36
42
50
50
47
1 1
1 1
2 3 2
63
37
48
39
54
36
100 100 100
107
96 97
E-6
-------
APPENDIX E
1991 BLACKSTOHE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DAXA
EPA Station # BI06 (River Mile 19.8)
DWPC Station f BS18
TOLERANCE ROCK BASKETS
VALUE
ABC
KICK NETS
ARTHROFODA
Insecta
Ephemeroptera (Mayflies)
Baetidae
Acentrella sp.
Baetis sp.
Heptageniidae
Stenonema sp.
Odonata
Anisoptera (Dragonflies)
Macromiidae
Macromia sp.
Zygoptera (Damselflies)
Coenagrionidae
Araia sp.
Enallaama sp.
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp.
Niaronia sp.
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatopavche sp.
Hvdropsvche sp.
Brachycentridae
Brachvcentrua sp.
Coleoptera (Beetles)
Elmidae (Riffle Beetles)
Ancvronvx sp.
Diptera (True Flies
Tipulidae (Crane Flies)
Antocha sp.
Tipula sp.
Chironomidae (Midge s)
Orthocladiinae
Cricotopus sp.
Orthocladius sp.
Tvetenia sp.
Chironominae
Rheotanvtarsus sp.
6
3
4
0
5
4
0.5
3 2
8 9 14
68
17
1 3
73
13
63
17
3 4 1
1
17 30 25
54
22
52 49
15 22
E-7
-------
APPENDIX E
BENTHIC MACROINVERTEBRATE DATA (Continued)
EPA Station # BI06 (River Mile 19.8)
DWPC Station # BS18
TOLERANCE ROCK BASKETS KICK NETS
VALUE*
ABC ABC
MOLLUSCA
Gastropoda (Snails)
Mesogastropoda
Basomatophora
Physidae
Phvsella sp. 8 11
Planorbidae
Belisoma sp. 6 3 4
Total # of Individuals: 100 100 100 104 108 103
E-8
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station < BI08 (River Mile 16.5)
DWPC Station < BS19
TOLERANCE
VALUE
ROCK BASKETS
ABC
KICK NETS
ARTHROPODA
Insecta
Ephemeroptera (Mayflies)
Siphlonuridae
Isonvchia sp.
Baetidae
Acentrella sp.
Baetis sp.
Heptageniidae
Stenonema sp.
Odonata
Zygoptera (Damselflies)
Coenagrionidae
Araia sp.
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp.
Niaronia sp.
Trichoptera (Caddisflies)
Philopotamidae
Chimarra sp.
Psychomyiidae
Psvchomvia sp.
Hydropsychidae
Cheumatopsvche sp.
HvdroDSvehe sp.
Macrostemum sp.
Hydroptilidae
Leucotrichia sp.
14 24
2
2
13 24
2
1
15
4
1
2
2
1
2
1
5-
44
32
30
29
21
34
4
28
36
21
37
35
31
3
5
11
2
3
4
3
6
1
4
4
6
E - 9
-------
APPENDIX E
BENTHIC MACROINVERTEBRATE DATA (Continued)
EPA Station # BI08 (River Mile 16.5)
DWPC Station # BS19
TOLERANCE ROCK BASKETS KICK NETS
VALUE*
A B C ABC
Diptera (True Flies)
Tipulidae (Crane Flies)
Antocha sp. 3
Chironomidae (Midges)
Orthocladiinae
Cardioe1adius sp. 5
Cricotopus sp. 7
Tvetenia sp. 5
Chironominae
Parachironomus sp. 10
Polvoedilum sp. 6
Empididae
Hemerodromia sp. 6
MOLLUSCA
Pelecypoda (Bivalves)
Heterodonta
Sphaeriidae
Sphaerium sp. 6 2 2
2
1 1
1
1 2
1
2 1
3 2
1
2
Total # of individuals 100 100 100 107 109 102
E-10
-------
APPENDIX E
1991 BIiACKSXONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BI09 (River Mile 9.8)
DWPC Station # BSRll
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
ARTHROPODA
Crustacea
Amphipoda
Gammaridae
Gammarus sp.
Insecta
Ephemeroptera (Mayflies)
Baetidae
Acentrella sp.
Baetis sp.
Heptageniidae
Stenonema sp.
Odonata
Zygoptera (Damselflies)
Coenagrionidae
Araia sp.
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp.
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatopsvche sp.
HvdroDsvche sp.
Macrostemum sp.
Hydroptilidae
Leucotrichia sp.
Leptoceridae
Oecetis sp.
Coleoptera (Beetles)
Hydrophilidae
Berosus sp.
Diptera (True Flies
Chironomidae (Midges)
Orthocladiinae
Cricotopus sp.
Nanocladius sp.
Tvetenia sp.
Chironominae
Dicrotendipes sp.
Glvatotendipes sp.
Polvpedilum sp.
Rheotanvtarsus sp.
7
3
5
8
10
6
6
1
1
12
5 53
4 32
3
6
5
52
39
1
48
37
25
45
22
50
1
2
12
23
53
E-l 1
-------
APPENDIX E
BENTHXC MACROINVERTEBRATE DATA (Continued)
EPA Station # BI09 (River Mile 9.8)
OWPC Station # BSRll
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
MOLLUSCA
Pelecypoda (Bivalves)
Heterodonta
Sphaeriidae
Sohaerium sp.
Total # of individuals
100 100 100
104 102 103
E-12
-------
APPENDIX E
1991 BLACXSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station BIOlO (River Mile 0.0)
DWPC Station t BSRI2
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC ABC
Insecta
Ephemeroptera (Mayflies)
Baetidae
Acentrella ap.
Baetis sp.
Heptageniidae
Stenonema sp.
Trichoptera (Caddisflies)
Hydropsychidae
Cheumatoosvche sp.
HvdroDsvche sp.
Coleoptera (Beetles)
Elmidae (Riffle Beetles)
Macronvchus sp.
Diptera (True Flies)
Tipulidae (Crane Flies)
Antocha sp.
Simuliidae (Black Flies)
Prosimulium sp.
chironomidae (Midges)
Orthocladiinae
Cardiocladius sp.
Cricotopus sp.
Nanocladius sp.
Orthocladius sp.
Tvetenia sp.
Chironominae
Glvototendipes sp.
Polvpedilum sp.
3
2
5
7
3
6
5
10
6
81
15
86
8
83
8
1
2
82
10
61
20
72
18
1 1
10 5
1
1
MOLLUSCA
Gastropoda (Snails)
Mesogastropoda
Basomatophora
Planorbidae
Helisoma sp.
Pelecypoda (Bivalves)
Heterodonta
Sphaeriidae
Sphaerium sp.
Total # of individuals
100 100 100
102 102 103
E-13
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BIOS:MR (River Mile 25.S/11.1)
DWPC Station # MF02
TOLERANCE ROCK BASKETS
VALUE
ABC
KICK NETS
ARTHROPODA
Amphipoda
Talitridae
Hvalella sp.
insecta
Ephemeroptera (Mayflies)
siphlonuridae
Isonvchia sp.
Baetidae
Acentrella sp.
Baetis sp.
Heptageniidae
Stenonema sp.
Odonata
Anisoptera (Dragonflies)
Aeschnidae
Boveria sp.
Zygoptera (Damselflies)
Coenagrionidae
Araia sp.
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp.
Trichoptera (Caddisflies)
Philopotamidae
Chimarra sp.
Polycentropodidae
Weureclipsis sp.
Hydropsychidae
Cheumatopsvche sp.
Hvdropsvche sp.
Leptoceridae
Oecetis sp.
28
14
8 20
1
4 9
23 25 19
111
13 14 11
4
21
41
16
10
4
7
7
2
1
3
1
1
2
5
9
7
15
4
-11
8
12
18
30
30
28
25
34
5
1
E-14
-------
APPENDIX E
BENTHIC MACROINVERTEBRATE DATA (Continued)
EPA Station # BI05:MR (River Mile 25.5/11.1)
DWPC Station # MF02
TOLERANCE ROCK BASKETS KICK NETS
VALUE
ABC A B C
Lepidoptera (Butterflies
and Moths)
Pyralidae
Parapovnx sp.
Coleoptera (Beetles)
Elmidae (Riffle Beetles)
Promoresia sp.
Diptera (True Flies)
Simuliidae (Black Flies)
Simulium sp.
Chironomidae (Midges)
Tanypodinae
Procladius sp.
Orthoc ladiinae
Nanocladius sp.
Orthocladius sp.
Rheocricotopus sp.
Tvetenia sp.
Chironominae
Rheotanvtarsus sp.
4.5
1
2
1
2
3
1
Total # of individuals
100 100 100
99 99 95
E-15
-------
APPENDIX E
1991 BLACKSTONE RIVER SURVEY
BENTHIC MACROINVERTEBRATE DATA
EPA Station # BI07:MI (River Mile 13.3/3.0)
DWPC Station # ML05
TOLERANCE ROCK BASKETS* KICK NETS**
VALUE
ABC ABC
ARTHROPODA
Insecta
isecta
Ephemeroptera (Mayflies)
Siphlonuridae
Isonvchia sp. _
Baetidae
Acentrella sp. 4 1
Baetia sp. 6 6 7
Heptageniidae
Stenonema sp. 25 29
Plecoptera (Stoneflies)
Perlidae
Acroneuria sp. Oil
Megaloptera
Corydalidae (Dobsonflies)
Corvdalus sp. 4 1
Niaronia sp. 0 1
Trichoptera (Caddisflies)
Philopotamidae
Chimarra sp.
Hydropsychidae
Cheumatopsvche sp.
Hvdropsvche sp.
Coleoptera (Beetles)
Psephenidae (Water Pennies)
Pseohenus sp.
chironomidae (Midges)
Chironominae
Rheotanvtarsus sp.
4
2
4
5
29
21
4
27
25
Total # of individuals 100 100
* only two of the three rock basket samples collected at this station were
analyzed.
**Kick-Net samples collected at this station were not analyzed.
E-l 6
-------
Appendix F
1991BLACKSTONE RIVER SURVEY
SEDIMENT OXYGEN DEMAND
-------
SEDIMENT OXYGEN DEMAND STUDIES
OF THE
BLACKSTONE RIVER
- SEPTEMBER 1992 -
Biology Section
Environmental Services Division
U.S. Environmental Protection Agency
Regional Laboratory
Lexington, Massachusetts
-------
INTRODUCTION
A sediment oxygen demand (SOD) study was undertaken on the
Blackstone River in central Massachusetts and Rhode Island, between September 1
and 10, 1992. This investigation was conducted by the U.S. Environmental
Protection Agency (EPA) for the purpose of developing data for the mathematical
model, QUAL 2E, to determine distinct oxygen regimes in the Blackstone River.
The EPA conducted analysis of sediments at ten (10) stations (station #s 92-1, 92-2A,
92-2B, 92-3, 92-4, 92-5, 92-6, 92-7, 92-8, and 92-9, which extended from Singing Dam in
Sutton, MA to Albion Dam in Cumberland, RI.
The method developed by the U.S. Environmental Protection Agency
Region I Biology Section involves confining a measurable volume of undisturbed
sediment and overlying water in a core cylinder and measuring the depletion of
dissolved oxygen over time. Sediment oxygen demand is considered the sum of
biological and chemical processes that exhibit an oxygen demand by the sediment
portion of the sample.
The core sample is collected using a gravity type corer (WILDCO No. 2402)
which is equipped with a plunger and a suction flap for use in deep water. In
shallow water the sample may be collected by manual coring. The acrylic core
cylinder measures 50.5 centimeters in height and 5.1 centimeters, inside diameter.
The cylinder is removed from the corer and stoppered at both ends after the
sediment sample is retrieved. This provides an undisturbed profile of
benthic sediments and overlying water.
Since the corer penetrates the substrate to variable depths depending on
sediment composition, both the sediment and water column contained within the
core must be precisely measured. Sediment surface area is always a constant in the
core cylinder. In cases where the sediment is soft or unconsolidated, the corer car.
penetrate the sediment entirely. This requires that some of the sediment be
carefully bled off the bottom of the cylinder. Overlying water may then be added to
fill the cylinder. The water column depth over the sediment is adjusted to a
minimum of 35 centimeters. Overlying water is also collected using a 3 L
Kemmerer sampler. Five replicate cores are collected at each sample location.
-------
Water column respiration or production is evaluated as follows: Six 300
ml BOD bottles are filled at the sample location. Half are fixed in the field and then
titrated in the laboratory according to the Winkler method. This establishes an
"Initial Blank" value. The remaining bottles are incubated in a shaded water bath
for the duration of the SOD test. Upon completion of the test, these are also titrated,
establishing a "Final Blank" value. Any measured difference between the "Initial"
and "Final" values are attributed to water column respiration or production and the
sediment oxygen demand rates are adjusted accordingly.
The water column height is measured and the cylinder is placed in a
temperature controlled water bath. Dissolved oxygen is monitored in each of the
core cylinders simultaneously using an Orbisphere five channel dissolved oxygen
meter (Model 2601). These five probes are calibrated daily against a Winkler
standard. The core tops are modified to accept the dissolved oxygen probes. A self-
contained stirring apparatus on each probe insures adequate flow past the sensing
head and also gives continuous mixing within the cylinder. Dissolved oxygen
concentrations within the cylinder are monitored for approximately three to four
hours and recorded every thirty minutes. This includes a one-half to one hour
period of temperature stabilization within the cylinder, when necessary. The water
temperature maintained in the water bath during this survey was 21 ±2 degrees
Celsius. This is the approximate temperature to be used in the waste load allocation
model.
Oxygen depletion is plotted against time. The portion of each graph where
oxygen consumption is constant over time is used in the calculation of the SOD rate.
-------
Sediment oxygen demand is calculated using the equation:
((Oi-Of) - (Bj-Bf)) (V)
SOD g02/m2/day = (SA) (T)
Oi = initial dissolved oxygen (DO) mg/L
Of = final DO mg/L
Bj = initial DO in bottles mg/L
Bf = final DO in bottles mg/L
V = volume of confined water m^
SA = sediment surface area m^
T = time davs
RESULTS
Table 1 lists the ten sample locations by station number, the location of the
station by town, the approximate distance upstream from Slater Mill, the.USGS
quad sheet on which the station may be located, the mean SOD (sediment oxygen
demand per gram of oxygen per meter squared per day) for each station calculated.,
and a brief description of the sediment type.
-------
Sta.#
Town
Location
River
Kilometers
USGS
Sheet
SOD
(Mean)
Sediment
Type
1
Sutton
Upstream from Singing dam
62.40
Grafton,
MA
6.026
black /oily
very fine
2A
Riverdale
Fond
Upstream from dam
51.80
Grafton,
MA
2.245
fine silt
2B
Riverdale
Pond
Upstream from dam
52.90
Grafton,
MA
5.777
fine silt
3
Rice City
Pond
Upstream from dam
45.45
Blackstone
, MA
1.773
black/oily
fine w/ sand
4
Rice Gty
Pond
Upstream from dam
44.95
Blackstone
,MA
3.190
black /oily
very fine
5
Millville
Rt. 122 between RR bridges-S
of Millville Ctr.
30.20
Blackstone
,MA
1.546
sandy/organic
6
Blackstone
Upstream from Tupperware
dam
29.00
Blackstone
,MA
1.551
sandy
7
Woonsocket
Downstream from Woonsocket
POTW
18:10
Pawtucket,
RI
5.789
very sandy
8
Cumberland
Upstream from Manville dam
16.05
Pawtucket,
RI
2.317
sandy/organic
9
Cumberland
Upstream from Albion dam
14.08
Pawtucket,
RI
2.523
sandy/organic
TABLE
Appendix A presents the tables of SOD rate calculations. The mean
sediment oxygen demand rate of each of five replicates is given at the end of each
block of station information. A standard deviation for this rate has also been
calculated. Appendix B includes figures which plot DO and temperature for each
core over time. Sediment oxygen demand rates for stations 1-9 ranged from a low
of 1.546 g/m2/d at station 5 to a high of 6.026 g/m2/d at station 1. Station locations
are identified in Figures 1 through 5 in Appendix C. Appendix D lists the raw data
in tables.
-------
APPENDIX A
-------
Station (No. 92-1
1
2
3
4
5
Initial sample Oxygen
7.05
5.47
6.1
7.2
6.17
(mg/L)
1
Final Sample Oxygen
6.03
4-21
3.82
5.5
2.84
(mg/L)
1
Initial Blank Oxygen
76
7.6
7.6
7.6
7.6
(ml/L)
Final Blank Oxygen
8.2
8.2
8.2
8.2
8.2
(ml/L)
,
Column height (m)
0.355
0.379
0.335
0.425
0.315
Time (Enter hrs.)
4
3
3
4
4
Time (days)
0.166666667
0.125
0.125
0.166666667
0.166666667
Surface Area (m2)
0.002
0.002
0.002
0.002
0.002
Volume (m3)
0.00071
0.000758
0.00067
0.00085
0.0006"
minus probe
0.0006
0.000648
0.00056
0.00074
0.00052
SODg/m2/d =
3.4506
5.66984
7.7184
5.865
7.4277
Mean SOD
€.026308
Std Dev
1.524102115
Station No. 92-2A
1
2
3
4
5
Initial sample Oxygen
6.52
6.7
5.78
5.88
5.85
(mg/L)
1
Final Sample Oxygen
5.71
5.65
5.27
5.06
4.04
(mg/L)
Initial Blank Oxygen
7
7
7
7
7
(ml/L)
Final Blank Oxygen
6.77
6.77
6.77
6.77
6.77
(ml/L)
1
Column height (m)
0.38
0.4
0.39
0.38
0.36
Time (Enter hrs.)
3.5
4
3.5
3.5
2.5
Time (days)
0.145833333
0.166666667
0.145833333
0.145833333
0.104166667
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.00076
0.0008
0.00078
0.00076
0.00072
minus probe
0.00065
0.00069
0.00067
0.00065
0.00061
SOD g/m2/d =
1.511314286
1.968
0.7488
1.537371429
5.4604S
Mean SOD
2J45193143
Std Dev
1.654913158
-------
Station No. 92-2B
1
2
3
4
5
Initial sample Oxygen
11.97
10.13
10.35
11.06
10.37
(mg/L)
Final Sample Oxygen
10.09
8.71
9.07
9.56
8.67
(mg/L)
Initial Blank Oxygen
11.03
11.03
11.03
11.03
11.03
(ml/L)
Final Blank Oxygen
11.26
11.26
11.26
11.26
11.26
(ml/L)
Column height (m)
0.405
0.37
0.25
0.4
0.43
Time (Enter hrs.)
2.5
3
2.5
3
3
Time (days)
0.104166667
0.125
0.104166667
0.125
0.125
Surface Area (m2)
0.002
0.002
0.002
0.002
0.002
Volume (m3)
0.00081
0.00074
0.0005
0.0008
0.00086
minus probe
0.0007
0.00063
0.00039
0.00069
0.00075
SODg/m2/d =
8.20368
4.884
3.624
5.536
6.6392
Mean SOD
5.777376
Std Dev
1.557119325
Station No. 92-3
1
2
3
4
5
Initial sample Oxygen
6.77
7.3| 7.42
5.52
7.54
(mg/L)
Final Sample Oxygen
6.01
6.43
6.54
5.17
6.45
(mg/L)
i
Initial Blank Oxygen
8.4
8.4
8.4
8.4
8.4
(ml/L)
Final Blank Oxygen
00
00
8.4
8.4
8.4
(ml/L)
Column height (m)
0.355
0.365
0.35
0.372
0.365
Time (Enter hrs.)
3
4.5
4.5
2.5
4.5
Time (days)
0.125
0.1875
0.1875
0.104166667
0.1875
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.00071
0.00073
0.0007
0.000744
0.00073
minus probe
0.0006
0.00062
0.00059
0.000634
0.00062
SODg/m2/d =
2.1584
1.6936
1.642666667
1.24992
2.121866667
Mean SOD
1.773290667
Std Dev
0.336792076
-------
Station No. 92-4
1
2
3
4
5
Initial sample Oxygen
5.81
7.71
7.43
6.91
7.02
(mg/L)
Final Sample Oxygen
4.57
7.05
6.39
6.28
6.12
(mg/L)
1
-
Initial Blank Oxygen
8
8
8
8
8
(ml/L)
f' ,
Final Blank Oxygen
8
8
8
8
8
(ml/L)
1
Column height (m)
0.305
0.34
0.32
0.36
0.32
Time (Enter hrs.)
2
" 3
2.5
3
1.5
Time (days)
0.083333333
0.125
0.104166667
0.125
0.0625
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.00061
0.00068
0.00064
0.00072
O.COC
minus probe
0.0005
0.00057
0.00053
0.00061
0.00053
SOD g/m2/d =
4.5384
1.7952
3.19488
1.8144
4.608
MeanSOD
3.190176
Std Dev
1.238278855
Station No. 92-5
1
2
3
4
5
Initial sample Oxygen
7.84
8.11
8.19
8.03
7.69
(mg/L)
1
Final Sample Oxygen
7.33
7.23
7.5
7.16
6.71
(mg/L)
1
Initial Blank Oxygen
8.2
8.2
8.2
8.2
8.2
(ml/L)
Final Blank Oxygen
8.06
8.06
8.06
8.06
8.06
(ml/L)
1
,
Column height (m)
0.4
0.393
0.377
0.387
0.37
Time (Enter hrs.)
3
4
4
4
4
Time (days)
0.125
0.166666667
0.166666667
0.166666667
0.166666667
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.0008
0.000786
0.000754
0.000774
0.00074
minus probe
0.00069
0.000676
0.000644
0.000664
0.00063
SOD g/m2/d =
1.184
1.74492
1.2441
1.69506
1.864S
MeaiiS01> 1.546576
Std Dev
0.277707559
-------
Station No. 92-6
1
2
3
4
5
Initial sample Oxygen
6.6 7
6.86
7.26
5.81
6.94
(mg/L) J
Final Sample Oxygen
6.2
6.25
6.76
4.98
6.35
(mg/L) I " - , '
Initial Blank Oxygen
8.2
8.2
8.2
8.2
8.2
(ml/L)
Final Blank Oxygen
8.2
8.2
8.2
8.2
8.2
(ml/L)
Column height (m)
0.37
0.332
0.343
0.319
0.36
Time (Enter hrs.)
3.5
3.5
3.5
2.5
3.5
Time (days)
0.145833333
0.145833333
0.145833333
0.104166667
0.145833333
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.00074
0.000664
0.000686
0.000638
0.00072
minus probe
0.00063
0.000554
0.000576
0.000528
0.00061
SOD g/m2/d =
1.192457143
1.388708571
1.176
2.541792
1.456457143
Mean SOD
1551082971
Std Dev
0.507173263
Station No. 92-7
1
2
3
4
5
Initial sample Oxygen
6.12
5.5
6.23
5.58
5.01
(mg/L)
Final Sample Oxygen
5.04
4.34
4.7
4.04
3.82
(mg/L) I
Initial Blank Oxygen
6.3
6.3
6.3
6.3
6.3
(ml/L) I
Final Blank Oxygen
6.86
6.86
6.86
6.86
6.86
(ml/L) f
Column height (m)
0.36
0.345
0.394
0.417
0.35
Time (Enter hrs.)
3
3
3
3
2.5
Time (days)
0.125
0.125
0.125
0.125
0.104166667
Surface Area
0.002
0.002
0.002
0.002
0.002
Volume
0.00072
0.00069
0.000788
0.000834
0.0007
minus probe
0.00061
0.00058
0.000678
0.000724
0.00059
SOD g/m2/d =
4.7232
4.7472
6.58768
7.0056
5.88
Mean SOD
5.788736
Std Dev
0.932476577
-------
Station No. 92-5
1
2
3
4
5
Initial sample Oxygen
6.59
7.25|
6.28
6.33
6.431
(mg/I.)
*
Final Sample Oxygen
6.13:
6.93:
5.54
5.78:
5.39
(me/L)
i
Initial Blank Oxygen
7.1
7.1
7.1
7.1,
7.1
-------
APPENDIX B
-------
30
20
a.
E
o
»-
01
E.
o
Q
10
0
-------
Data from "SINGING DAM\SOD92-1\d"
TEMPI
TEMP 2
TEMP 3
TEMP 4
TEMP 5
120
MINUTES
240
-------
Data from "RIVERDALE POND\SOD92-2B\dM
DO 1
TEMP 1
DO 2
TEMP 2
DO 3
TEMP 3
DO 4
TEMP 4
DO 5
TEMP 5
210
MINUTES
-------
Data from "RICE CITY POND\SOD92-3\d"
B
D01
•
TEMPI
O—
DO 2
•
TEMP 2
DO 3
TEMP 3
*
DO 4
*
TEMP 4
¦
DO 5
t
TEMP 5
60 90 120 150 180 210
MINUTES
-------
Data from "RICE CITY POND\SOD92-4\d"
30 T s
20
O
o
CL
E
-------
Data from "MILVlLLE\SOD92-5\d"
1
1
1 —
a— D01
«— TEMP 1
o— DO 2
•— TEMP 2
O— DOS
a— TEMP 3
*— DO 4
*— TEMP 4
¦— DO 5
1 TEMPS
h—
r1
)
|
I
;
|
!
i
1
i
1
no 60 90 120 >50 J80 210 240
MINUTES
-------
Data from "MILVILLE\SOD92-6\dM
TEMPI
TEMP 2
TEMP 3
TEMP 4
TEMP 5
21C
MINUTES
-------
Data from ,,MANVILLE\SOD92-7\d,
30
20 •
O
a.
E
a
E
O
a
10 •
DO 1
TEMP 1
DO 2
TEMP 2
DO 3
TEMP 3
DO 4
TEMP 4
DO 5
TEMP 5
30
60
90
MINUTES
120
150
180
-------
Data from MMANVILLE\SOD92-8\d"
o
o
D01
TEMP 1
DO 2
TEMP 2
DO 3
TEMP 3
DO 4
TEMP 4
DOS
TEMP 5
MINUTES
-------
Data from "ALBION \SOD92-9\d"
TEMP 1
TEMP 2
TEMP 3
TEMP 4
TEMP 5
Column 13
90
MINUTES
180
-------
APPENDIX C
-------
(Cy fcr Pond**
.Tw'*?' ¦ r? Caiumeti-
ockvilte
R
Figure 3. Stations 3 & 4.
-------
£fie«tn
on ©
as*?
x\
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iv «>
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Z%/iUah 1.
O
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&
,S3>
-" X
CSiSO
-L'7
'M>
a-v m
Yft
-o
o«
o
ItCfi
-7imy >
*.'' T/.'
Kennedy
Ml
i \m
wwmm
VSfc
\\ \o
v>
otacKSton
* \T^\
ion
rSNO
\VV:
an
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Blac
•HS. •
ml&.
SSACTUSg
ISW.ND -
3t»\ \ tWiw ^
Waterfo
MM
mw^m.
MJl
m
wmrm
203 •
WW
I 37*1.
Halliwell
Memorial Sch
rtOSD
KtnH^lf*u
Figure 4. Stations 5 & 6.
-------
'v /f.
g?fmm
( -.Y'vV A
* '*!'• />">
•^cri'Av
rrpl .
^^jSJjt Sneeeh
"1|§I Pond 0^-
^^%W\ WrUMVS
[cm//
&: J L^WJZX'
Cumberland •" X * • V-';
a x V . * %\ * C i • ..
&.&o<'
'.vv,-:v- r • •
V >•.*.* \
V •"."•!•'*.*
2/'; V.1.^ % \
1^/tI
i.oV
-:V»>'. Vj- ¦. iV i, •-* i •VyTS a 2"?J 1
Figure 5. Stations 7, 8, & 9.
Figure 5. Stations 7, 8, & 9
-------
APPENDIX D
-------
APPcnnrru
TIME MINUIES DO I TEMP I D02 TEMP2
1
2
Station 1
3
1030
0
7.050
22.000
5.750
22.100
4
1100
30
6.900
22.300
5.640
22.400
5
1130
60
6.780
22.500
5.470
22.600
6
1200
90
6.650
22.100
5.280
22.300
7
1230
120
6.540
22.300
5.100
22.300
0
1300
150
6.370
22.500
4.870
22.600
9
1330
180
6.250
22.700
4.640
22.700
10
1400
210
6.150
22.600
4.420
22.700
11
1430
240
6.030
22.700
4.200
22.700
12
Station 2A
13
1035
0
6.700
20.900
6.700
20.900
14
1105
30
6.520
22.000
6.570
22.000
15
1135
60
6.400
22.400
6.440
22.500
16
1205
90
6.280
22.600
6.320
22.700
17
1235
120
6.150
22.900
6.180
22.900
18
1305
150
6.030
23.200
6.050
23.200
19
1335
180
5.910
22.800
5.900
22.800
20
1405
210
5.820
22.800
5.770
22.800
21
1435
240
5.710
22.900
5.650
22.900
22
Station 2B
23
1500
0
13.330
23.100
10.570
22.900
24
1530
30
12.530
23.000
10.130
22.000
25
1600
60
11.970
22.600
9.830
22.400
26
1630
90
11.570
22.900
9.600
22.600
27
1700
120
11.180
23.000
9.350
22.900
28
1730
150
10.840
23.400
9.150
23.100
29
1800
180
10.450
23.500
8.920
23.300
30
1830
210
10.090
23.900
8.710
23.600
31
Station 3
32
1040
0
7.150
19.800
7.300
19.500
33
1110
30
6.770
21.800
7.200
21.600
34
1140
60
6.600
22.500
7.110
22.400
35
1210
90
6.450
22.700
6.990
22.700
36
1240
120
6.330
23.000
6.890
22.900
37
1310
150
6.220
22.900
6.800
22.700
38
1340
180
6.100
22.800
6.700
22.700
39
1410
210
6.010
23.100
6.610
22.800
40
1440
240
3.970
23.000
6.510
22.900
41
1510
270
5.950
23.200
6.430
23.200
DO 3
6.640
6.360
6.100
5.750
5.420
5.000
4.590
4.210
3.S20
6.230
5.700
5.680
5.610
5.530
5.460
5.380
5.330
5.270
11.400
10.750
10.350
10.050
9.780
9.550
9.300
9.070
7.420
7.320
7.210
7.110
7.000
6.910
6.810
6.720
6.630
6.540
TLMI' 3
22.100
22.400
22.600
22.300
22.400
22.500
22.000
22.600
22.500
20.600
21.900
22.400
22.600
22.900
23.100
22.000
22.000
22.900
23.000
22.900
22.500
22.600
22.800
23.100
23.300
23.500
19.600
21.600
22.400
22.700
22.900
22.600
22.600
22.800
23.000
23.200
DO 4
7.200
6.990
6.770
6.530
6.330
6.100
5.900
5.700
5.500
6.040
5.880
5.750
5.630
5.510
5.390
5.270
5.170
5.060
11.700
11.060
10.820
10.590
10.330
10.070
9.810
9.560
4.600
4.530
4.020
6.460
5.520
5.470
5.420
5.320
5.280
5.170
11 MP -I
21 BOO
22.200
22.400
22.100
22.200
22.400
22.500
22 500
22.500
20.400
21.700
22.200
22.500
22.700
22.000
22.600
22.600
22.700
22.700
22.700
22.200
22.400
22.600
23.000
23.200
23.500
19.300
21.300
22.200
22.600
22.800
22.600
22.600
22.600
22.700
22.900
IKM)
6.170
5.820
5.330
4.900
4.530
4.130
3.650
3.240
2.040
6.400
6.400
6.070
5.850
5.480
5.080
4.690
4.360
4.040
10.840
10.370
9.980
9.660
9.400
9.160
8.910
8.670
7.540
7.440
7.300
7.160
7.030
6.910
6.790
6.680
6.560
6.450
H.MP 'j
22.300
22.300
22.500
22.200
22.300
22.600
22.700
22.600
22.600
20.200
21 800
22.300
22.600
22.900
23.200
22.800
22.000
23.000
23.100
23.000
22.600
22.700
22.900
23.200
23.400
23.700
19.400
21 600
22.400
22.700
22.900
22.600
22.600
22.800
22.900
23.100
-------
APPENDIX D1
TIME MINUTES DO 1 TEMPI 002 TEMP 2
1
2
Station 4
3
1530
0
7.250
18.900
7.710
18.700
4
1600
30
6.410
21.700
7.630
21.800
5
1630
60
5.810
22.700
7.510
22.800
6
1700
90
5.390
23.000
7.380
23.100
7
1730
120
5.070
23.100
5.260
23.200
8
1800
150
4.780
23.300
7.130
23.400
9
1830
ISO
4.570
23.400
7.050
23.400
10
Station 5
1 1
1115
0
8.120
20.100
8.110
20.400
12
1145
30
8.000
22.000
8.030
22.000
13
1215
60
7.840
22.500
7.920
22.500
14
1245
90
7.710
22.800
7.780
22.800
15
1315
120
7.620
22.700
7.630
22.700
16
1345
150
7.520
22.500
7.520
22.600
17
1415
180
7.460
22.700
7.420
22.700
16
1445
210
7.380
22.500
7.320
22.600
19
1515
240
7.330
22.600
7.230
22.700
20
Station 6
21
1500
0
13.330
23.100
10.570
22.900
22
1530
30
12.530
23.000
10.130
22.800
23
1600
60
11.970
22.600
9.830
22.400
24
1630
90
11.570
22.900
9.600
22.600
25
1700
120
11.180
23.000
9.350
22.900
26
1730
150
10.840
23.400
9.150
23.100
27
1800
180
10.450
23.500
8.920
23.300
20
1830
210
10.090
23.900
8.710
23.600
29
Station 7
30
1030
0
6.120
23.200
5.500
22.800
31
1100
30
5.930
23.000
5.300
22.800
32
1130
60
5.740
23.200
5.090
23.000
33
1200
90
5.580
23.400
4.910
23.300
34
1230
120
5.390
23.500
4.720
23.400
35
1300
150
5.220
23.700
4.530
23.600
36
1330
180
5.040
24.000
4.340
23.900
Wif-I, 0o<; 02,
0 39 I'M
DO 3
TEMP 3
DO 4
TEMP 4
DO 5
I'tMP 5
7.430
7.270
7.000
6.750
6.560
6.390
6.260
IB.BOO
21.800
22.800
23.200
23.200
23.500
23.500
6 910
6.810
6.680
6.560
6.450
6.350
6.280
18.400
21.700
22 600
22 900
22.900
22.100
22.200
G.r,r,o
7.o;'0
6.680
6.360
6.120
5.190
5.750
10.HOO
21.700
22.600
23.000
23.200
23.300
23.500
8.190
8.110
8.000
7.900
7.800
7.700
7.640
7.560
7.500
20.400
22.100
22.600
22.900
22.700
22.500
22.600
22.600
22.700
8.030
7.900
7.760
7.610
7.500
7.400
7.310
4.480
7.160
20.100
21.800
22.400
22.700
22.500
22.300
22.400
22.900
22.500
7.690
7.580
7.440
7.270
7.150
7.020
6.910
6.800
6.710
20.000
21.900
22.500
22.900
22.600
22.500
22.500
22.500
22.600
11.480
10.750
10.350
10.050
9.780
9.550
9.300
9.070
23.000
22.900
22.500
22.600
22.800
23.100
23.300
23.500
11.700
11.060
10.820
10.590
10.330
10.070
9.810
9.560
22.700
22.700
22.200
22.400
22.600
23.000
23.200
23.500
10.840
10.370
9.980
9.660
9.400
9.160
8.910
8.670
23.100
23.000
22.600
22.700
22.900
23.200
23.400
23.700
6.230
5.970
5.670
5.430
5.190
4.950
4.700
22.900
22.600
22.800
23.000
23.100
23.300
23.500
5.580
5.310
5.030
4.790
4.530
4.290
4.040
22.700
22.600
22.700
22.900
23.000
23.100
23.400
5.340
5.010
4.770
4.550
4.290
4.050
3.820
23.300
22.900
22.900
23.100
23.200
23.400
23.700
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1
2
3
4
5
6
7
8
9
10
1 t
12
13
14
15
16
17
18
18
APPENDIX Di!
TIME
MINUTES
DO 1
TEMP 1
DO 2
TEMP 2
DO 3
TEMP 3
DO -I
ILMI'-t
Station 8
1530
1600
1630
1700
1730
1800
1830
Station 9
1115
1145
1215
1245
1315
1345
1415
1445
1515
0
30
60
90
120
150
180
0
30
60
90
120
150
180
210
240
7.250
6.410
5.810
5.390
5.070
4.780
4.570
8.120
8.000
7.840
7.710
7.620
7.520
7.460
7.380
7.330
10.900
21.700
22.700
23.000
23.100
23.300
23.400
20.100
22.000
22.500
22.800
22.700
22.500
22.700
22.500
22.600
7.710
7.630
7.510
7.380
5.260
7.130
7.050
8.110
8.030
7.920
7.780
7.630
7.520
7.420
7.320
7.230
18.700
21.800
22.800
23.100
23.200
23.400
23.400
20.400
22.000
22.500
22.800
22.700
22.600
22.700
22.600
22.700
7.430
7.270
7.000
6.750
6.560
6.390
6.260
8.190
8.110
8.000
7.900
7.800
7.700
7.640
7.560
7.500
18 B00
21.800
22.800
23.200
23.200
23.500
23.500
20.400
22.100
22.600
22.900
22.700
22.500
22.600
22.600
22.700
6.910
6 810
6 680
G 560
6.450
6.350
6.280
8.030
7.900
7.760
7.610
7.500
7.400
7.310
4.480
7.160
10.400
21.700
22 600
22.900
22.900
22.100
22.200
20.100
21.000
22.400
22.700
22.500
22.300
22.400
22.900
22.500
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