910R93014B
united States
Environmental Protection
Agency
Region 10
1200 Sixth Avenue
Seattle WA98101
Alaska
Idaho
Oregon
Washington
Water Division
Surface Water Branch
August 1993
oEPA Refinements of
Current PSDDA Bioassays
Final Data Package
'„,a:
I
v %"
'* 1i
-------
EPA DOCUMENT NUMBER: EPA 910/R-93-014b
DATA PACKAGE
REFINEMENTS OF CURRENT PSDDA BIOASSAYS
FINAL REPORT
EPA Contract No. 68-C8-0062
Work Assignment No. 3-57
March 19. 1993
Report No. 9210.003/B.016
SAIC Project No. 01-0098-03-1009
Submitted to:
U.S. Environmental Protection Agency
26 West Martin Luther King Drive
Cincinnati, Ohio 45268
Submitted by:
Science Applications International Corporation
Environmental Sciences Division
18706 North Creek Parkway, Suite 110
Bothell, WA 98011
IMHUBl
RXO0DQO3b?Q
An fmp/oyee-OwnW Company
-------
PHASE II OYSTER
, amr Uw«_
An Employee-Owned Company
-------
LARVAL COUNTS
An Employee-Owned Company
-------
Bioassay Organism Larval Counts - Oyster Phase II (NH3 Unaerated)
Cone.
(mg/L)
Sample
Number
Normal
Abnormal
% Mortality
T=0
183
T=0
153
T=0
140
T=0
158
T=0
179
T=0
172
T=0
153
T=0
205
T=0
162
T=0
195
mean=
170
SW (final)
1A
122
4
25.9%
IB
134
2
20.0%
SW (final)
2A
121
3
27.1%
2B
114
0
32.9%
SW (final)
3A
113
4
31.2%
3B
128
4
22.4%
SW (final)
4A
149
0
12.4%
4B
157
2
6.5%
SW (final)
5A
SPILLED BEFORE
COUNTED
5B
149
3
10.6%
1
1A
150
2
10.6%
1
IB
133
4
19.4%
1
2A
135
3
18.8%
1
2B
152
1
10.0%
1
3A
104
1
38.2%
1
3B
105
1
37.6%
1
4A
117
5
28.2%
1
4B
129
2
22.9%
1
5A
124
5
24.1%
I
SB
156
4
5.9%
5
1A
303
3
Probable
5
IB
185
5
-11.8%
5
2A
112
1
33.5%
5
2B
139
0
18.2%
5
3A
140
3
15.9%
5
3B
127
5
22.4%
5
4A
144
7
11.2%
5
4B
148
12
5.9%
5
5A
94
1
44.1%
5
5B
100
4
38.8%
Replicate %
Mortality
Mean Mean
Sample % Standard Replicate % Sample % Standard
Mortality Deviation % Abnormality Abnormality Abnormality Deviation
22.9%
30.0%
26.8%
9.4%
10.6%
15.0%
14.4%
37.9%
25.6%
15.0%
double innoculation. Data discarded
-11.8%
25.9%
19.1%
8 5%
41.5%
19.9% 8.4%
21.6% 9.2%
11.9% 16.8%
3.2%
1.5% 2.3%
2.4%
0.0% 1.2%
3.4%
3.0% 3.2%
0.0%
1.3% 0.6%
2.0% 2.0%
1.3%
2.9% 2.1%
2.2%
0.7% 1.4%
1.0%
0.9% 0.9%
4.1%
1.5% 2.8%
3.9%
2.5% 3.2%
2.6% 2.6%
0.9%
0.0% 0.4%
2.1%
3.8% 2.9%
4.6%
7.5% 6.1%
1.1%
3.8% 2.4%
1.9% 0.9%
2.1% 0.8%
1.8% 2.0%
-------
Bioassay Organism Larval Counts - Oyster Phase II (NH3 Unaerated) (continued)
'one.
Sample
ng/L)
Number
Normal
Abnormal
% Mortality
10
1A
2
20
87.1%
10
IB
2
20
87.1%
10
2A
0
9
94.7%
10
2B
0
5
97.1%
10
3A
1
14
91.2%
10
3B
0
14
91.8%
10
4A
1
29
82.4%
10
4B
0
22
87.1%
10
5A
0
17
90 0%
10
5B
2
25
84.1%
20
1A
0
17
90.0%
20
IB
0
10
94.1%
20
2A
0
2
98.8%
20
2B
0
2
98.8%
20
3A
0
2
98.8%
20
3B
0
3
98.2%
20
4A
0
3
98.2%
20
4B
0
2
98.8%
20
5A
0
1
99.4%
20
5B
0
0
100.0%
40
1A
0
0
100.0%
40
IB
0
1
99.4%
40
2A
0
1
99.4%
40
2B
0
3
98.2%
40
3A
SPILLED BEFORE
COUNTED
40
3B
0
0
100.0%
40
4A
0
0
100.0%
40
4B
0
2
98.8%
40
5A
0
0
100.0%
40
5B
0
0
100.0%
Sample Sample % Mortality
and Abnormality
% Abnormality
SW 19 9* 1.9%
1 21.6% 2.1%
5 11.9% 1.8%
10 89.2% 96.4%
20 97.5% 100.0%
40 99 6% 100 0%
Replicate %
Mortality
Mean Mean
Sample % Standard Replicate % Sample % Standard
Mortality Deviation % Abnormality Abnormality Abnormality Deviation
87.1%
95.9%
91.5%
84.7%
87.1%
92.1%
98.8%
98.5%
98.5%
99.7%
99.7%
98.8%
100.0%
99.4%
100.0%
89.2%
4.0%
97.5%
2.8%
90.9%
90.9%
100.0%
100.0%
93.3%
100.0%
96.7%
100.0%
100.0%
92.6%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
90.9%
100.0%
96.7%
98.3%
96.3%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
96.4%
3.1%
100.0%
0.0%
99.6%
0.4%
100.0%
0.0%
-------
Bioassay Organism larval Counts - Oyster (NH3 Aerated)
Cone.
(mg/L)
Sample
Number
Normal
Abnormal
% Mortali'
T=0
183
T=0
153
T=0
140
T=0
158
T =0
179
T=0
172
T=0
153
T=0
205
T =0
162
T=0
195
mean=
170
SW (final)
1A
136
1
19.4%
IB
134
1
20.6%
SW (final)
2A
127
1
24.7%
2B
150
2
10.6%
SW (final)
3A
143
3
14.1%
3B
133
0
21.8%
SW (final)
4A
104
0
38.8%
4B
91
0
46.5%
SW (final)
5A
120
0
29.4%
5B
136
1
19.4%
1
1A
191
1
-12.9%
1
IB
322
3
-91.2%
1
2A
134
2
20.0%
1
2B
138
1
18.2%
1
3A
96
1
42.9%
1
3B
136
1
19.4%
1
4A
127
1
24.7%
1
4B
131
1
22.4%
1
5A
131
2
21.8%
1
5B
125
1
25.9%
5
1A
140
3
15.9%
5
IB
110
2
34.1%
5
2A
107
3
35.3%
5
2B
108
2
35.3%
5
3A
93
4
42.9%
5
3B
137
2
18.2%
5
4A
78
3
52.4%
5
4B
106
0
37.6%
5
5A
128
5
21.8%
5
5B
104
2
37.6%
Mean Mean
Replicate % Sample % Standard Replicate % Sample % Standard
Mortality Mortality Deviation % Abnormality Abnormality Abnormality Deviation
20.0%
17.6%
17.9%
42.6%
24.4%
-52.1%
19.1%
31.2%
23.5%
23.8%
25.0%
35.3%
30.6%
45.0%
29.7%
24.5%
9.4%
9.1%
30.8%
23.7%
16.0%
0.7%
0.7%
0.8%
1.3%
2.1%
0.0%
0.0%
0.0%
0.0%
0.7%
0.5%
0.9%
1.5%
0.7%
1.0%
0.7%
0.8%
0.8%
1.5%
0.8%
2.1%
1.8%
2.7%
1.8%
4,1%
1.4%
3.7%
0.0%
3.8%
1.9%
0.7%
1.0%
1.0%
0.0%
0.4%
0.7%
1.1%
0.9%
0.8%
1.1%
1.9%
2.3%
2.8%
1.9%
2.8%
0.6%
0.4%
0.9%
0.2%
1.5%
1.2%
-------
Bioassay Organism larval Counts - Oyster (NH3 Aerated) (continued)
Cone.
Sample
(mg/L)
Number
Normal
Abnormal
% Mortality
10
1A
0
3
98.2%
10
IB
2
8
94.1%
10
2A
0
1
99.4%
10
2B
0
4
97.6%
10
3A
0
2
98.8%
10
3B
0
0
100.0%
10
4A
0
0
100.0%
10
4B
0
1
99 4%
10
5A
0
0
100.0%
10
5B
0
0
100.0%
20
1A
0
2
98.8%
20
IB
0
0
100.0%
20
2A
0
0
100.0%
20
2B
0
0
100.0%
20
3A
0
0
100.0%
20
3B
0
1
99.4%
20
4A
0
1
99.4%
20
4B
0
0
100.0%
20
5A
0
3
98.2%
20
5B
0
0
100.0%
40
1A
0
1
99 4%
40
IB
0
0
100.0%
40
2A
0
0
100.0%
40
2B
0
0
100.0%
40
3A
0
2
98.8%
40
3B
0
0
100.0%
40
4A
0
0
100 0%
40
4B
0
0
100.0%
40
5A
0
0
100.0%
40
5B
0
0
100.0%
Bioassay Organism larval Counts
- Oyster (NH3 Aerated) (continued)
Sample
Sample % Mortality
and Abnormality
% Abnormalil
SW
24.5%
0.6%
1
9.1%
0 9%
5
23.7%
1.5%
10
98.8%
97.5%
20
99.6%
100 0%
40
99.8%
100 0%
Mean Mean
Replicate % Sample % Standard Replicate % Sample % Standard
Mortality Mortality Deviation % Abnormality Abnormality Abnormality Deviation
100.0%
96.2% 80.0% 90.0%
100.0%
98.5% 100.0% 100.0%
100.0%
99.4% 100.0%
99.7% 100.0% 100.0%
100.0% 98.8% 1.4% 97.5% 4.3%
100.0%
99.4% 100.0%
100.0%
99.7% 100.0% 100.0%
100.0%
99.7% 100.0%
100.0%
99.1% 99.6% 0.3% 100.0% 100.0% 0.0%
100.0%
99.7% 100.0%
100.0%
100.0%
99.4% 100.0%
100.0%
100.0% 99.8% 0.2% 100.0% 0.0%
-------
Bioassay Organism Larval Counts - Oyster (Cadmium Reference Test (NH3))
Cone. Sample
(mg/L) Number Normal Abnormal % Mortality
sw
1A
122
0
28.2%
IB
135
1
20.0%
sw
2A
120
2
28.2%
2B
109
4
33.5%
0.75
1A
32
5
78.2%
IB
28
9
78.2%
0.75
2A
20
6
84.7%
2B
19
11
82.4%
1.5
1A
8
11
88 8%
IB
15
13
83.5%
1.5
2A
7
7
91.8%
2B
20
20
76.5%
3.0
IA
0
17
90.0%
IB
0
21
87.6%
3.0
2A
1
7
95.3%
2B
1
8
94.7%
6.0
1A
5
10
91.2%
IB
4
9
92.4%
6.0
2A
1
4
97.1%
2B
0
S
97.1%
Mean Mean
Replicate % Sample % Standard Replicate % Sample % Standard
Mortality Mortality Deviation % Abnormality Abnormality Abnormality Deviation
0.0%
24.1% 0.7% 0.4%
1.6%
30.9% 27.5% 3.4% 3.5% 2.6% 1.5% 1.1%
13.5%
78.2% 24.3% 18.9%
23.1%
83.5% 80.9% 2.6% 36.7% 29.9% 24.4% 5.5%
57.9%
86.2% 46.4% 52.2%
50.0%
84.1% 85.1% 1.0% 50.0% 50.0% 51.1% 1.1%
100.0%
88.8% 100.0% 100.0%
87.5%
95.0% 91.9% 3.1% 88.9% 88.2% 94.1% 5.9%
66.7%
91.8% 69.2% 67.9%
80.0%
97.1% 94.4% 2.6% 100.0% 90.0% 79.0% 11.0%
-------
AMMONIA DATA
An Employee-Owned Company
-------
Exper Num
920702 DATE 8-Jul-92
DAY T-0 rer Filename
07Q2rtp
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
85.5
27.9
-57.6
1
9.26
before samples
82.0
25.9
-56.1
-56.85
2
9.27
-56.33
3
9.28
(assumes pressure of
1 atm.)
79.4
23.6
-55.8
4
9.29
after samples
79.4
23.6
-55.8
-55.80
5
6
9.30
9.32
7
9.33
8
9.34
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added
w Std
ppm
C.
PPt
pH factor
ppm
NH3 ppm Mean
T-0 control
5.0
170.9 over cut-off std.
7.95 I 1.00
N.D.
N.D.
T-0 1mg/l
5.0
118.1
50
107.4
50
20.0
28
7.97 1.00
o.8§
0.03
T-0 5mg/l
5.0
76.0
390
63.0
50
20.0
28
7.95 1.00
4.68
0.13
T-0 10mg/I
5.0
57.0
900
45.1
50
20.0
28
7.93 1.00
9.79
0.27
T-0 20mg/l
5.0
39.3
180
23.9
500
20.0
28
7.89 1.00
19.06
0.48
T-0 40mg/l
5.0
22.0
390
6.5
500
20.0
28
7.81 1 1.00
37.81
0.80
Temperature and salinity values were not specifically taken in each of these
individual jars; These are the assumed values.
pH values are mean pH of aerated and unaerated jars for each concentration.
N.D. = Non Detection. Value was below method detection limit.
-------
Exper Num
Phase II Oyster: Calculation of Total and Unionized Ammonia - Orion Probe Data
920702 DATE 7-Jul-92 DAY T = 4 Filename 0702TO
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
91.8
34.3
-57.5
1
9.26
before samples
89.0
32.2
-56.8
-57.15
2
9.27
-56.73
3
9.28
(assumes pressure of 1 atm.)
83.3
26.6
-56.7
4
9.29
after samples
82.4
26.5
-55.9
-56.30
5
6
9.30
9.32
7
9.33
8
9.34
If samples were not diluted, enter 1 for dilution factor
Sample Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read diluted
added
mv
added
w Std
ppm
C.
PPt
PH
factor
ppm
NH3 ppm Mean
T-4 Control A
5.1
lost "132
20.9
28
7.95
1.00
N.D.
N.D.
T-4 Control UA
5.1
above
cut off
20.9
27
7.96
1.00
N.D.
N.D.
T-4 1 mg/l A
5.1
119.6
50
110.3
50
20.8
27
7.96
1.00
1.04
0.03
T-4 lmg/l UA
5.1
119.9
50
110.8
50
20.9
28
7.93
1.00
1.06
0.03
T-4 5mg/l A
5.1
78.8
310
68.7
50
20.9
28
7.97
1.00
5.08
0.16
T-4 5mg/l UA
5.1
78.2
310
68.0
50
21.1
28
7.93
1.00
5.02
0.15
T-4 10mg/l A
5.1
60.7
720
51.0
50
20.9
28
7.99
1.00
10.20
0.34
T-4 10mg/l UA
5.1
60.7
720
51.1
50
21.1
27
7.92
1.00
10.31
0.30
T-4 20mg/l A
5.1
42.7
160
28.6
500
20.8
28
7.95
1.00
18.94
0.58
T-4 20 mg/l U
5.1
42.1
170
27.7
500
21.0
28
7.89
1.00
19.52
0.53
T-4 40mg/l A
5.1
24.5
310
12.5
500
21.0
28
7.84
1.00
41.83
1.01
T-4 40mg/l UA
5.1
24.3
310
12.0
500
21.1
28
7.82
1.00
40.65
0.95
T-4 Control A*
5.1
"141
*20
50
20.9
28
7.95
1.00
N.D.
N.D.
* T-4 Control A-mcl std added too late
** T-4 Control A, redone. Collected at 5.5 hrs after stocks
were made ( 1.5 hrs after innoculationl.
N.D.= Non detection. Value was below method detection limit.
Phase ((Oyster: Calculation of Total and umowzea Ammonta - vnvn nuo«
-------
Exper Wum
920702 DATE
8-Jul-92
DAY t-4 reru Filename
0702rto
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
92.4
34.6
-57.8
1
9.26
before samples
87.6
31.0
-56.6
-57.20
2
9.27
-56.75
3
9.28
83.8
27.1
-56.7
4
9.29
after samples
82.6
26.7
-55.9
-56.30
5
9.30
6
9.32
7
8
9.33
9.34
(assumes pressure of 1 atm.l
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
PPt
pH factor
ppm
NH3 ppm
Mean
T-4 control
5.0
" 166 above cut-off std.
1.00
N.D.
N.D.
T-4 control
5.0
" 173 above cut-off std.
1.00
N.D.
N.D.
T-4 1mg/l
5.0
121.5
50
93.2
200
20.0
28
7.92
1.00
0.92
0.02
T-4 1mg/l
5.0
118.2
50
109.4
50
20.0
28
7.91
1.00
1.13
0.03
T-4 5mg/l
5.0
78.6
310
67.3
50
20.0
28
7.89
1.00
4.56
0.12
T-4 10mg/l
5.0
60.1
700
50.3
50
20.0
28
7.91
1.00
10.05
0.27
T-4 20mg/l
5.0
42.1
170
27.2
500
20.0
28
7.93
1.00
19.04
0.53
T-4 40mg/l
5.0
24.9
310
11.9
500
20.0
28
7.82
1.00
38.76
0.84
n. Value was below method detection limit.
-------
Exper Num
Phase II Oyster: Calculation of Total and Unionized Ammonia -
920702 DATE 10-Jul-92 DAY t = 48 Filename
Orion Probe Data
0702T48
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
82.9
25.7
-57.2
1
9.26
before samples
82.1
26.2
-55.9
-56.55
2
9.27
-56.78
3
9.28
82.0
25.5
-56.5
4
9.29
after samples
83.2
25.7
-57.5
-57.00
5
9.30
6
9.32
7
9.33
8
9.34
(assumes pressure of 1 atm.)
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
ppt
pH factor
ppm
NH3 ppm
Mean
T-48 Control A
5.0
144.8
above
cut off
20.7
28
8.08
1.00
N.D.
N.D.
T = 48 ControlU
5.0
140.1
above
cut off
20.7
27
8.03
1.00
N.D.
N.D.
T-48 1 mg/1 A
5.0
116.0
90
101.4
50
20.7
27
8.07
1.00
1.07
0.04
T-48 1 mg/l U
5.0
114.6
90
99.5
50
20.6
28
8.00
1.00
1.02
0.03
T = 48 5mg/l A
5.0
76.5
380
63.4
50
20.7
28
8.06
1.00
4.58
0.18
T-48 5mg/l UA
5.0
75.8
380
63.4
50
20.8
28
8.00
1.00
4.88
0.17
T = 48 10mg/I
5.0
58.6
800
48.4
50
20.5
28
8.07
1.00
10.61
0.41
T = 48 10mg/l U
5.0
57.6
800
46.7
50
20.8
28
8.00
1.00
9.94
0.34
T = 48 20mg/l
5.0
40.9
130
28.6
500
20.8
27
7.97
1.00
18.85
0.60
T = 48 20mg/l U
5.0
40.8
130
28.7
500
20.6
28
7.97
1.00
19.23
0.61
T = 48 40mg/l
5.0
24.5
310
12.2
500
20.7
28
8.08
1.00
41.39
1.68
T = 48 40mg/l U
5.0
24.5
310
12.1
500
20.8
28
7.92
1.00
41.00
1.17
N.D.= Non detection. Value was below method detection limit.
-------
Exper Num
920702 DATE 10-Jul-92
DAY T-24 Filename 0702T24
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
80.0
24.1
-55.9
1
9.26
before samples
78.5
23.3
-55.2
-55.55
2
9.27
-55.53
3
9.28
79.2
23.6
-55.6
4
9.29
after samples
79.6
24.2
-55.4
-55.50
5
9.30
6
9.32
7
8
9.33
9.34
(assumes pressure of 1 atm.)
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
PPt
pH factor
ppm
NH3 ppm
Mean
*T-24 control a
5.0
134.3
50
130.6
50
20.0
28
8.01
1.00
N.D.
N.D.
*T-24 control u
5.0
138.3
50
129.4
50
20.0
28
7.99
1.00
N.D.
N.D.
T-24 1mg/l a
5.0
113.3
100
97.0
50
20.0
29
8.01
1.00
0.99
0.03
T-24 1mg/l ua
5.0
111.7
100
96.7
50
20.0
28
7.97
1.00
1.11
0.03
T-24 5mg/l a
5.0
72.3
470
58.2
50
20.0
28
8.01
1.00
4.88
0.16
T-24 5mg/l ua
5.0
72.4
470
58.4
50
20.0
28
7.96
1.00
4.92
0.15
T-24 10mg/l a
5.0
55.2
1000
42.6
50
20.0
28
8.03
1.00
9.77
0.34
T-24 10mg/l ua
5.0
54.9
1000
42.6
50
20.5
28
7.96
1.00
10.02
0.31
T-24 20mg/J a
5.0
38.6
190
21.6
500
20.0
28
7.96
1.00
17.26
0.51
T-24 20mg/l ua
5.0
37.6
190
21.3
500
20.0
28
7.93
1.00
18.26
0.50
T-24 40mg/l a
5.0
19.9
400
4.2
500
20.5
29
7.96
1.00
37.35
1.14
T-24 40mg/l ua
5.0
20.3
400
5.2
500
20.0
28
7.87
1.00
39.21
0.95
Ph values are averages of T-4hr values and T-8 values.
N.D.= Non detection. Value was below method detection limit.
* Mv started to increase before added standards.
-------
PHYSICAL MONITORING DATA
An Employee-Owned Company
-------
Client
Experiment #
PHASE n BIVALVE PHYSICAL MONITORING DATA
EPA TESC 3-57
920702 & 920701 (CD Ref)
Day 0 Day 1 Day 2
Date 7-Jul-92 8-Jul-92 9-Jul-92
Time
Observer M. Pelletier, D. MacBridc C. Sheehan, M. Pelltier, J. Dinsdale J. Dinsdale, M. Pelltier, J. Dinsdale
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
NH3 ASW
20.9
28
7.2
7.95
20
28
7.3
8.01
20.7
28
6.9
8.08
NH3 UA SW
20.9
28
7.1
7.95
20
28
7.1
7.99
20.7
27
6.9
8.03
NH3 A 1
20.8
29
7.3
7.97
20
29
7.4
8.01
20.7
27
7.0
8.07
NH3 UA 1
20.9
28
7.3
7.97
20
28
7.1
7.97
20.6
28
6.5
8
NH3 A 5
20.9
28
7.2
7.95
20
28
7.2
8.01
20.7
28
6.9
8.06
NH3UA 5
21.1
28
7.3
7.95
20
28
7.4
7.96
20.8
28
7.0
8
NH3 A 10
20.9
28
7.3
7.93
20
28
7.3
8.03
20.5
28
6.9
8.07
NH3 UA 10
21.1
27
7.2
7.93
20.5
28
7.0
7.96
20.8
28
7.0
8
ND3 A 20
20.8
28
7.1
7.89
20
28
7.4
7.%
20.8
27
6.9
7.97
NH3 UA 20
21
28
7.2
7.89
20
28
7.3
7.93
20.6
28
6.8
7.97
NH3 A 40
21
29
7.3
7.81
20.5
29
7.4
7.96
20.7
28
6.8
8.08
NH3 UA 40
21.1
28
7.2
7.81
20
28
7.2
7.87
20.8
28
6.8
7.92
Cd Low 0.75
22.1
28
7.1
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
CD Hi 6.0
21.7
28
7.1
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
-------
REFERENCE TOXICANT
An Employee-Owned Company
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PHASE II OYSTER EC
Observed
Adjusted
Predicted
Number
Number
Proportion
Proportion
Proportion
Cone.
Exposed
Resp.
Responding
Responding
Responding
Control
100
2
0.0200
0.0000
0.0221
0.7500
100
24
0.2400
0.2228
0.1827
1.5000
100
51
0.5100
0.4989
0.5810
3.0000
100
94
.0.9400
0.9386
0.9056
Chi - Square Heterogeneity = 4.871
***************************************************************
* WARNING *
* *
* Significant heterogeneity exists. The results reported *
* for this data set may not be valid. The results should *
* be interpreted with appropriate caution. *
***************************************************************
***************************************************************
* NOTE *
* *
* Slope not significantly different from zero. *
* EC fiducial limits cannot be computed. *
***************************************************************
MU = 0.120626
Sigma = 0.271251
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 4.555298 0.238727 ( 1.522038, 7.588557)
Slope 3.686620 0.867590 ( -7.336982, 14.710221)
Spontaneous 0.022106 0.032379 ( -0.389306, 0.433518)
Response Rate
-------
PHASE II OYSTER EC
Estimated EC Values and Confidence Limits
Lower Upper
Point Cone. 95% Confidence Limits
EC 1.00
0.3088
EC 5.00
0.4725
EC10.00
0.5929
EC15.00
0.6910
EC50.00
1.3202
EC85.00
2.5220
EC90.00
2.9394
EC95.00
3.6881
EC99.00
5.6447
-------
KASE II OYSTER EC
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Jrobit
10+
9+
8+
7+
6+
4 +
3 +
2 +
1+
• •
5+ . . . o
o.
0+
+ + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
PHASE II ECHINODERM
JPBET
An Emptoyee-Ownecf Company
-------
LARVAL COUNTS
An Employee-Owned Company
-------
Bioassay Organism larval Counts - Echinoderm Phase II
Ammonia
Concentration
(mS/L>
Normal
% Mortality and Replicate % Station % Standard % Replicate % Station % Standard
Abnormal Abnormality Mortality Mortality Deviation Abnormality Abnormality Abnormality Deviation
Initial Counts
233
189
254
223
250
230
Unaerated Seawater Control
0.275
0.625
198
205
208
198
165
189
202
187
211
162
173
210
214
189
150
210
201
193
194
189
175
188
202
213
197
189
147
142
14
15
30
27
34
24
13
18
35
35
40
31
24
34
42
28
36
20
29
47
37
30
25
15
26
16
35
37
7.7%
4.3%
-3.6*
2.1%
13.4%
7.3%
6.4%
10.8%
-7.0%
14.3%
7.3%
-4.9%
-3.6%
3.0%
16.4%
-3.6%
-3.1%
7.3%
3.0%
-2.7%
7.7%
5.1%
1.2%
0.8%
3.0%
10.8%
20.8%
22.1%
6.0%
-0.7%
10.4%
8.6%
3.6%
1.2%
-0.3%
6.4%
2.1%
0.1%
6.4%
1.0%
6.9%
21.5%
5.6%
6.6%
1.9%
6.5%
6.3%
9.3%
6.6%
6 8%
12.6%
12.0%
17.1%
11.3%
6.0%
8.8%
14.2%
17.8%
18.8%
12.9%
10.1%
15.2%
21.9%
11.8%
15.2%
9.4%
13.0%
19.9%
17.5%
13.8%
11.0%
6.6%
11.7%
7.8%
19.2%
20.7%
6.7%
12.3%
14.2%
7.4%
16.0%
15.8%
12.7%
16.8%
12.3%
16.5%
15.6%
8.8%
9.7%
20.0%
11.3%
4.0%
14.8%
4.0%
10.3%
6.5%
-------
Unaerated Seawater Control (continued)
Ammonia
Concentration
Normal Abnormal
£ Mortality and
Abnormality
Replicate %
Mortality
180
235
13
21
16.0*
-11.4%
2.3%
1.25
162
179
198
169
168
207
170
165
165
181
24
18
23
26
18
24
35
32
29
31
19.1%
14.3%
3.8%
15.1%
19.1%
-0.5%
10.8%
14.3%
15.6%
7.7%
16.7%
9.5%
9.3%
12.5%
11.7%
2.5
53
74
82
72
70
66
46
58
162
143
129
140
108
136
137
135
146
146
62
58
20.8%
6.9%
17.3%
9.5%
9.9%
12.5%
16.4%
11.2%
2.5%
12.5%
13.8%
13.4%
11.2%
13.8%
7.5%
203
211
211
213
195
198
190
206
209
218
11.7%
8.2%
6.9%
7.3%
15.1%
13.8%
17.3%
10.4%
8.2%
5.1%
9.9%
7.1%
14.5%
13.8%
6.7%
10
227
219
0.8%
4 3%
2.5%
Station % Standard % Replicate % Station % Standard
Mortality Deviation Abnormality Abnormality Abnormality Deviation
11.9%
12.0%
10.4%
6.9%
5.0%
3.8%
7.2%
6.7%
8.2%
12.9%
9.1%
10.4%
13.3%
9.7%
10.4%
17.1%
16.2%
14.9%
14.6%
70.9%
65.4%
56.8%
65.4%
66.2%
67.2%
76.0%
71.6%
27.7%
28.9%
100.0%
100.0%
98.6%
100.0%
100.0%
100 0%
100.0%
100.0%
99.1%
100.0%
99.6%
99.5%
7.5%
11.0%
11.9%
10.0%
16.7%
14.8%
68.1%
61.1%
66.7%
73.8%
28.3%
100.0%
99.3%
100.0%
100.0%
99.5%
99.6%
12.9%
59.6%
99.8%
83.1%
2.7%
16.4%
0.5%
37.2%
-------
Undented Seawater Control (continued)
Ammonia
Concentration % Mortality and Replicated
(mg/L) Normal Abnormal Abnormality Mortality
0
175
23.8%
12.3%
0
228
0.8%
0
211
8.2%
10.4%
0
201
12.5%
0
196
14.7%
7.7%
2
226
0.8%
1
219
4.3%
8.2%
1
201
12.1%
Station %
Mortality
Standard
Deviation
%
Abnormality
Replicate % Station % Standard
Abnormality Abnormality Deviation
100.0% 100.0%
100.0%
100.0% 100.0%
100.0%
100.0% 99.6%
99.1%
99.5% 99.5%
99.5%
-------
Bioasaay Organism larval Counts - Echinoderm Phase II(Aerated Seawater Control)
Ammonia
Concentration
% Mortality and Replicate % Station % Standard % Replicate % Station % Standard
Normal Abnormal Abnormality Mortality Mortality Deviation Abnormality Abnormality Abnormality Deviation
220
202
214
226
235
231
203
218
237
209
9
6
4
6
5
3
5
3
3
3
0.3%
9.5%
5.1%
-1.0%
-4.4%
-1.8%
9.5%
3.8%
-4.4%
7.7%
4.9%
2.1%
-3.1%
6.7%
1.7%
2.4%
5.2%
3.9%
2.9%
1.8%
2.6%
2.1%
1.3%
2.4%
1.4%
1.3%
1.4%
3.4%
2.2%
1.7%
1.9%
1.3%
2.1%
0.8%
0.275
215
246
209
207
200
202
208
168
216
200
4.3%
-9.2%
5 6%
8.2%
9.9%
8.2%
7.3%
23.4%
4.7%
12.5%
-2.5%
6.9%
9.1%
15.4%
8.6%
7.5%
7.7%
2.3%
2.0%
3.7%
1.9%
3.4%
4.3%
2.3%
4.5%
1.4%
0.5%
2.1%
2.8%
3.8%
3.4%
0.9%
2.6%
1.2%
0.625
205
219
202
211
202
200
223
249
204
212
5
5
6
9
9
13
10
3
8
13
8.6%
2.5%
9.5%
4.3%
8.2%
7.3%
-1.4%
-9.7%
7.7%
2.1%
5.6%
6.9%
7.7%
-5.5%
4.9%
3.9%
5.6%
2.4%
2.2%
2.9%
4.1%
4.3%
6.1%
4.3%
1.2%
3.8%
5.8%
2.3%
3.5%
5.2%
2.7%
4.8%
3.7%
1.5%
1.25
198
197
215
9.9%
10.4%
3.8%
10 1%
3.8%
2.0%
13 9%
4.3%
4.4%
2.7%
4.4%
2.7%
2.6%
1.5%
-------
Bioassay Organism larval Counts - Echinoderm Phase ll(Aerated Seawaler Control) (continued)
Ammonia
Concentration % Mortality and Replicate %
(mg/L) Normal Abnormal Abnormality Mortality
1% 3 13.4%
314 5 -38.8% -23.4%
241 7 -7.9%
188 5 16.0% 11.4%
208 6 6.9%
201 9 8.6% 4.9%
218 9 1.2%
2.5 124 89 7.3% 10.6%
141 57 13.8%
191 86 -20.5% -12.7%
143 98 -4.9%
147 60 9.9% 10.6%
145 59 11.2%
138 75 7.3% 6.0%
144 75 4.7%
140 61 12.5% 17.1%
110 70 21.7%
5 0 218 5.1% 2.3%
1 230 -0.5%
1 240 -4.9% -4.4%
0 239 -4.0%
1 207 9.5% 11.2%
0 200 13.0%
0 201 12.5% 12.8%
2 198 13.0%
0 231 -0.5% 1.9%
1 219 4.3%
10 1 233 -1.8% -2.3%
1 235 -2.7%
1 225 1.7% 0.8%
0 230 -0.1%
0 219 4.7% 11.2%
0 189 17.8%
0 194 15.6% 11.4%
0 213 7.3%
0 224 2.5% 6.9%
1 203 11.2%
Station % Standard % Replicate % Station % Standard
Mortality Deviation Abnormality Abnormality Abnormality Deviation
6.3%
11.0%
4.7%
6.7%
5.6%
6.8%
1.5%
1.6%
2.8%
2.6%
2.8%
4.3%
4.0%
41.8%
28.8%
31.0%
40.7%
29.0%
28.9%
35.2%
34.2%
30.3%
38.9%
100.0%
99.6%
99.6%
100.0%
99.5%
100.0%
100.0%
99.0%
100.0%
99.5%
99.6%
99.6%
99.6%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
99.5%
2.2%
2.7%
4.1%
35.3%
35.9%
29.0%
34.7%
34.6%
99.8%
99.8%
99.8%
99.5%
99.8%
99.6%
99.8%
100.0%
100.0%
99.8%
33.9%
4.8%
99.7%
0.3%
99.8%
0.2%
-------
Bioassay Organism larva! Counts - Echinoderm Phase il (Cadmium Reference Test)
Ref Tox
-------
AMMONIA DATA
An Employee-Owned Company
-------
Laboratory Analysis Record
Page 1 of 2
Parametrix, Inc.
Analysis:
Organism:
Project Name:
Project Number:
Instrument Calibrations
Ammonia
Ecfainoderms
EPA Larval
Media:
Round #:
Test Date:
55-1738-42
Unit 001: Orion Model #72QA
Unit 002: Orion Model #250A
Seawater
1/8/92
Staff:
Date:
Start Time:
End Time:
DCK
1/8/92
0755
1443
Orion Unit #
001) Specific Ion Probe #
002) Specific Ion Probe #
001
95-12
Ammonia Standards (SOP #PMX-AN-3)
100 ppm (NHj-N)
x 10 ppm (NHj-N)
x 1.0 ppm (NH,-N)
x 0.1 ppm (NH,-N)
Serial #
Serial #
20R04
_0.05 ppm (NHj-N)
_0.01 ppm (NHj-N)
_See Control Charts (reference)
Slope % -59.7 mV (-56 to -60 mV/decade)
Slope % (-56 to -60 mV/decade)
NBS Standard
Orion Cat. No. 951207 @ 100 ppm
Lot # VW1 Date N/A
Method Detection Limit =* 0.01 ppm NH,-N
Lab Temp:
Time (hn)
» Sample I.D.
NH,-N
(ppm)
%
Recovery
tnV
Time (hn)
Sample I.D.
NHyN
(ppm)
%
Recovery
mV
0755
01 Blank
0.00
1218
1.0 Std
1.05
0815
D1 Blank
0.00
1220
10 Std
10.5
0825
0.01 CS
1300
DI Blank
0.00
0827
0.10 CS
1311
1006
0.00
1.0 CS
1314
1015
0.21
10.0 CS
Slope ¦
•59.8
1319
0.01 Std
0.00
0842
OI Blank
0.00
1320
0.10 Std
0.10
0851
0.01 Std
0.01
1322
1024
0.63
0854
0.1 Std
0.10
132S
1033
1.14
0855
1.0 Std
0.98
1328
1042
1.82
0859
10 Std
9J8
1330
1051
4.26
0906
DI Blank
0.00
133S
1060
7.64
1010
0.01 Std
0.01
1341
1069
0.01
1012
0.10 Std
0.09
1344
1078
0.27
1026
Lab SW wtftmtn.
0.00
1348
1087
0.53
1031
Lafc SW wio fnmrt.
0.00
1350
1096
1.24
1041
10 Std
9.10
1352
HQS
131
1102
LakSW WpnMV.
0.00
13S5
1114
3.86
1214
0.01 Std
0.01
1358
1123
732
1216
0.1 Std
0.10
CS » Calibration Standard
MB « Method Blank (daily with each wawater batch)
DUP - Duplicate (laboratory) (1 in 20)
MS » Spike AnalyiU (laboratory) (1 in 20)
RS - Replicate Sample (field) (1 in 20)
SPB - Sample Presentation Blanks
SOW ¦ Synthetic Ocean Water (for standards preparation)
Std • Ammonia Standard (processed; 28 ppt)
SW » Seawater (0.2 tim filtered; LTV precused; 28 ppt)
-------
Laboratory Analysis Record
Page 2 of 2
Parametrix, Inc.
Analysis:
Organism:
Project Name:
Project Number:
Instrument Calibrations
Ammonia
Echinoderms
EPA Larval
Media:
Round #:
Test Date:
55-1738-42
Unit 001: Orion Model #720A
Unit 002: Orion Model #25QA
Seawater
1/8/92
Staff:
Date:
Start Time:
End Time:
DCK
1/8/92
0755
1443
Orion Unit #
001) Specific Ion Probe #
002) Specific Ion Probe #
001
95-12
JL
Serial #
Serial #
20R04
Ammonia Standards (SOP #PMX-AN-3)
100 ppm (NHj-N)
x 10 ppm (NHj-N)
x 1.0 ppm (NHj-N)
x 01 ppm (NHj-N)
0.05 ppm (NH,-N)
.0.01 ppm (NHj-N)
.See Control Charts (reference)
Slope % -59.7 mV (-56 to -60 mV/decade)
Slope % (-56 to -60 mV/decade)
NBS Standard
Orion Cat. No. 951207 @ 100 ppm
Lot # VW1 Date N/A
Method Detection Limit = 0.01 ppm NH,-N
Lab Temp: 22.3 °C
Time (lus)
Sample I.O.
NH,-N
(ppm)
%
Recovery
mV
Time (his)
Sample I.D.
NH.-N
(ppm)
%
Recovery
mV
1411
1123 Dup
6J3
1414
1123 MS
7.03
93
1429
1087 Dup
0.48
1430
1087 MS
1.32
89
1436
0.01 Std
0.00
1439
0.1 Std
0.09
1441
1.0 Std
0.94
1443
10 Std
9.00
CS ¦ Calibration Standard
MB • Method Blank (daily with each tea water batch)
DUP * Duplicate (laboratory) (1 in 20)
MS ¦ Spike Analysis (laboratory) (1 in 20)
RS - Replicate Sample (field) (1 in 20)
SPB ¦ Sample Preservation Blanks
SOW - Synthetic Ocean Water (for standards preparation)
Std - Ammonia Standard (processed; 28 ppt)
SW « Seawater (0.2
-------
Laboratory Analysis Record
Page 1 of 2
Parametrix, Inc.
Analysis:
Organism:
Project Name:
Project Number:
Instrument Calibrations
Ammonia
Echinoderms
EPA Larval
Media:
Round M:
Test Date:
55-1738-42
Unit 001: Orion Model 1720A
Unit 002: Orion Model #250A
Seawater
1/9-1/10/92
Staff:
Date:
Start Time:
End Time:
BV, DCK
1/10/92
0907
1436
Orion Unit #
001) Specific Ion Probe It
002) Specific Ion Probe It
001
95-12
Ammonia Standards (SOP (CPMX-AN-3)
100 ppm (NH j-N)
x 10 ppm (NH ,-N)
__1_1.0 ppm (NH j-N)
x 0.1 ppm (NH ,-N)
_0.05 ppm (NH ,-N)
_5 0.01 ppm (NH ,-N)
Serial It
Serial tt
20R04
_5 See Control Charts (reference)
Slope % -58.1mV (-56 to -60 mV/decade)
Slope % (-56 to -60 mV/decade)
NBS Standard
Orion Cat. No. 951207 (® 100 ppm
Lot# VW1 Date N/A
Method Detection Limit =* 0.01 ppm NH j-N
Lab Temp: 24J XL
Tuna (hr*)
Sampla I.O.
NHj-N
(ppm)
%
Recovery
mV
Tima (hrt)
Sampla I.D.
NHrN
(ppm)
%
Recovery
mV
T24 (1/9/92)
1056
1053
3.29
0907
DI Blank
0.00
1058
1062
8.00
0916
0.01 CS
0.01
1105
1071
0.02
0918
0.1CS
0.09
1107
1080
0.20
0919
1.0CS
1.01
1109
1089
0.43
0921
IOCS
10.1
Slops -
-58.7
1111
1098
0.84
0929
DI Blank
0.00
1114
1107
1.66
0931
0.01 Std
0.01
1116
1116
3.66
0934
0.1 Std
0.09
1118
1125
7.11
0936
1.0 Sid
0.99
1140
1044 Dup
1.65
0938
lO.OSld
9.84
1141
1044 IMS
2.50
94
0957
1008
0.02
T48 (1/10/92)
0959
1017
0.24
1315
0.01Std
0.00
1001
1026
0.51
1317
0.1 Std
0.09
1003
1035
1.01
1319
1.0 Std
0.91
1048
0.01 Sid
0.01
1320
10 Std
9.43
1050
O.lOStd
0.09
1346
1009
0.02
1051
1.0 Sid
0.95
1348
1018
0.20
1052
10 Sid
9.32
1350
1027
0.48
1054
1044
1.92
1351
1036
0.94
CS " Calibration Standard
MB " Method Btenk (daily with aach aeawaiar bitch)
QUP m Duplicate (laboratory) (1 in 20)
MS * Spike Analyaia (laboratory) (1 in 20)
m a Replicau Samp la (field) (1 in 20)
SPB ¦ Sample Preurvaiioa Blanka
SOW - SymiMtie Ocean Watar (for aandaids preparation)
Sid > Ammonia Standard (procesaad; 28ppt)
SW m Saawatar (0.2 tin fiharad; ITV proeaaaad; 28 ppt)
-------
Laboratory Analysis Record
Page 2
Parametrix,
BY, PCX
1/10/92
0907
1436
Instrument Calibrations Unit 001: Orion Model #720A
Unit 002: Orion Model #250A
Analysis: Ammonia Media: Seawater Staff:
Organism: Echinoderms Round #: Date:
Project Name: EPA Larval Test Date: 1/9-1/10/92 Start Time:
Project Number: 55-1738-42 End Time:
Orion Unit #
001) Specific Ion Probe ft
002) Specific Ion Probe ft
001
95-12
Ammonia Standards (SOP /CPMX-AN-3)
100 ppm (NH j-N)
x 10 ppm (NH j-N)
x 1.0 ppm (NH ,-N)
x 0.1 ppm (NH ,-N)
0.05 ppm (NH j-N)
x 0.01 ppm (NH j-N)
Serial #
Serial #
20R04
_5 See Control Charts (reference)
Slope % -58.1 mV (-56 to -60 mV/decade)
Slope % (-56 to -60 mV/decade)
NBS Standard
Orion Cat. No. 951207 @ 100 ppm
Lot It VW1 Date N/^
Method Detection Limit =¦ 0.01 ppm NH ,-N
Lab Temp: 24.5 °C
Time (hra)
Sample l.D.
NHfN
(ppm)
%
Recovery
mV
Time (hra)
Sample l.D.
NHfN
(ppm)
%
Recovery
mV
13S3
1045
1.82
1357
1054
3.15
1400
1063
6.05
1402
1072
0.03
1406
1081
0.20
1411
1090
0.34
1413
1090Dup
0.35
1416
1090 MS
1.08
80
1417
1099
0.76
1419
1108
1.52
1421
1117
3.07
1423
1126
6.08
1430
0.01 Std
0.00
1432
O.lOStd
0.08
1434
1.0 Std
0.92
1436
lO.OStd
9.0
CS m Calibration Standard
MB » Method Blank (daily with each aeawaiar buck)
DUP ¦ Dupiicata (laboratory) (I in 20)
MS « Spike Analyiis (laboratory) (1 in 20)
R3 » Replicate Sample (field) (1 in 20)
SPB ¦ Sampla Praaarvation Blanki
SOW - Synthetic Ocean Watar (for Madaida preparation)
Std » Ammonia Standard (procaiaad: 28 ppt)
SW - Sea watar (0.2 pn filtered; UV prooaaMd; 28 ppt)
-------
PHYSICAL MONITORING DATA
Ar Employee-Owned Company
-------
PHASE II ECHINODERM PHYSICAL MONITORING DATA
Client
Experiment #
Day 0 Day 1 Day 2
Date 8-Jan-92 9-Jan-92 10-Jan-92
Time
Observer
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
Unaerated
0
14
28
7.8
7.7
13.5
28
7.6
7.6
15.5
28
7.6
7.6
0.275
14
28
7.5
7.7
13.5
28
7.6
7.7
15.5
28
7.6
7.7
0.625
14
28
7.8
7.7
13.5
28
7.5
7.6
15.5
28
7.5
7.7
1.25
14
28
7.7
7.7
13.5
28
7.5
7.6
15.5
28
7.5
7.7
2.5
14
28
7.7
7.7
13.5
28
7.6
7.7
15.5
28
7.5
7.6
5
14
28
7.6
7.7
13.5
28
7.7
7.7
15.5
28
7.6
7.6
10
14
28
7.6
7.7
13.5
28
7.6
7.6
15.5
28
7.7
7.7
Aerated
0
14
28
7.5
7.7
13.5
28
7.6
7.9
15.5
28
7.7
7.9
0.275
14
28
7.6
7.7
13.5
28
7.6
7.8
15.5
28
7.8
7.8
0.625
14
28
7.6
7.7
13.5
28
7.6
7.9
15.5
28
7.7
7.8
1.25
14
28
7.7
7.7
13.5
28
7.7
7.8
15.5
28
8.1
7.8
2.5
14
27
7.6
7.7
13.5
28
7.7
7.9
15.5
28
7.7
7.8
5
14
28
7.6
7.7
13.5
28
7.6
7.8
15.5
28
7.8
7.8
10
14
28
7.6
7.7
13.5
28
7.5
7.8
15.5
29
8.1
7.8
Ref Tox (CdC12 as Cd)
12 mg/L
14
28
7.6
7.7
13.5
28
7.5
7.6
15.5
28
7.8
7.6
-------
REFERENCE TOXICANT
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PHASE II ECHINODERM ABNORMALITY.
REFERENCE TOXICANT CdC12
Observed
Adjusted
Predicted
Number
Number
Proportion
Proportion
Proportion
Cone.
Exposed
Resp.
Responding
Responding
Responding
Control
100
4
0.0400
0.0000
0.0429
3.0000
100
12
0.1200
0.0806
0.0612
6.0000
100
17
0.1700
0.1328
0.1661
12.0000
100
39
0.3900
0.3627
0.3464
Chi - Square Heterogeneity = 1.109
MU = 1.286163
Sigma = 0.523797
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 2.544538 0.451900 ( 1.658814, 3.430261)
Slope 1.909137 0.489701 ( 0.949324, 2.868951)
Spontaneous 0.042863 0.020074 ( 0.003519, 0.082208)
Response Rate
-------
PHASE II ECHINODERM PROBIT ANALYSIS
Estimated EC Values and Confidence Limits
Lower Upper
Point Cone. 95% Confidence Limits
EC 1.00
1.1686
0.1474
2.3762
EC 5.00
2.6581
0.7512
4.2050
EC10.00
4.1197
1.7584
5.8040
EC15.00
5.5373
3.0523
7.3787
EC50.00
19.3269
13.5827
47.0533
EC85.00
67.4569
32.8379
552.2949
EC90.00
90.6695
40.1590
996.5966
EC95.00
140.5259
54.0193
2393 . 7427
EC99.00
319.6477
93.9036
12422.9971
-------
PHASE II ECHINODERM EC
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4 +
3 +
2 +
1+
0+
. o.
• • • • o
o. . .
-+ + + + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
PHASE 1IIA
JSHP
, ham*_
An Employee-Owned Company
-------
REFERENCE SEDIMENT CONVENTIONAL DATA
An Employee-Owned Company
-------
ANALYTICAL
RESOURCES
INCORPORATED
31 July 1992
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Sandy Browning
SAIC
18706 North Creek Parkway. Suite 110
Bothell, WA 98011
RE: Project No. EPA 3-57 (Carr Inlet) / ARI Job #B013
Dear Ms. Browning:
Please find enclosed original reports for the above referenced project. Four
soil/sediment samples were analyzed for conventional parameters, as reauested.
The samples were extracted and analysis was completed following PSEP protocols' far
PSDDA analytes. The sediment grain size distribution was subcontracted fa Soil'
Technology, Inc.
As always, copies of these reports and all associated raw data will remain on file with. ARI!
If you have any questions or require additional information , please contact me at your
convenience.
Sincerely,
ANALYTICAL RESOURCES, INC.
Susan D. Rosa Dunnihoo
Project Manager
Enclosures
cc: File B013
-------
SOIL
TC-MCLOGY
LETTER OF TRANSMITTAL
TO: Analytical Resources, Inc. DATE: July 9, 1992
3 33 Ninth Avenue North
Seattle, WA 98109-5187 JOB NO: J-250
ATTENTION: John Hicks
SUBJECT: Sediment Grain Size Distribution
Carr Inlet EPA-3-57
REFERENCE: Chain of Custody Record dated 6/23/92
Lab ID No. B013A through B013D
We are sending the following items:
DATE
COPIES
DESCRIPTION
07/09/92
2
Sediment Grain Size Distribution Table
07/09/92
1
Chain of Custody Record
These are transmitted for your use.
REMARKS: Samples were tested in general accordance with Puget
Sound Estuary Protocol (Conventional Sediment Variables Particle
Size March 1986). Values reported are "apparent" particle size as
organic material is included in the analysis.
Best regards,
SOIL TECHNOLOGY, INC.
Richard G. Sheets,
Vice President
-------
ANALYTICAL
RESOURCES
INCORPORATED
Final Report
Laboratory Analysis of Sediment Parameters (PSEP)
Sample Matrix: SEP!
Data Release Authorized:
Report Prepared : July 9, V.
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle. WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Project No: EPA 3-57 (Carr Inlet)
QC Report No: SAIC B013
Date Received: 6/22/92
DATE OF ANALYSIS
SAMPLE DATA SUMMARY
7/7/92 | 7/2/92
7/2/92
7/9/92 I 7/9/92
7/9/92
6/24/92
6/24/92
SOLIDS
TOC (Dry Wt)
TOC
Dry Wt IMC
<%a
Sample Number
PURCED
(%«70O
TOTAL
<% • IMC)
TVS
(ppm dry wt)
TOC (70a
(ppmC)
TOC (IMC)
(ppm C)
AMMONIA
(ppm N)
SULFIDE
(ppm)
Lab ID
B013 A
CARR4
63.96%
62.59%
13,700
4,500
4,404
0.4
3.72
011
B013 B
CARR2
66.85%
64.74%
12700
4,280
4,145
0.4
74.85
0.13
B013 C
CARR6
35.52%
3249%
21300
13,200
1Z074
1.2
9.87
0.15
B013 D
CARR A
77.43%
78.32%
10,100
6,110
6,180
0.6
Z59
0.10
QC DATA SUMMARY
PURCED
TOTAL
TVS
TOC (70C)
TOC (IMC)
Dry Wt IMC
AMMONIA
SULFIDE
Sample Number
(%«7oa
(%eiMO
(ppm dry wt)
(ppmC)
(ppmC)
(%a
(mn/L)
(ppm)
Method Blank 1
-
46.0
-
-
0.108
<0.0024
Method Blank 2
-
-
-
-
-
<0.0024
Detection Limit
-
-
57.4
-
-
0.04
0.0024
Check Standard Analysis:
Replicate/
PURCED
TOTAL
TVS
TOC (70C)
TOC (IMC)
Dry Wt IMC
AMMONIA
SULFIDE
(%«7oa
(% • IMC)
(ppm dry wt)
(ppm C)
(ppmC)
(%a
(ppm N)
(mffS)
Measured
1,949
-
9.56
0.45
Measured
-
-
-
-
-
0.37
"True"
-
2,000
-
10.00
0.58
% Recovery
-
97.45%
-
-
95.61%
70.74%
ysis:
PURGED
TOTAL
TVS
TOC (70O
TOC (IMC)
Dry Wt IMC
AMMONIA
SULFIDE
<%o7oa
(% • 104C)
(ppm dry wt)
(ppmC)
(ppm C)
(%a
(ppm N)
(ppm)
Original
63.96%
6159%
13.700
4,500
-
-
2.59
<0.11
Duplicate
64.03%
63.11%
13300
4,800
-
-
2.41
<0.12
Triplicate
63.40%
5.010
-
-
-
USD
0.54%
0.59%
210%
5.37%
-
5.21%
-
Spike Analysis
PURCED
TOTAL
TVS
TOC (70C)
TOC (IMC)
Dry Wt IMC
AMMONIA
SULFIDE
(%»7oa
(% • IMC)
(ppm dry wt)
(ppmC)
(ppmC)
(%a
(ppm N)
(ppm)
Original
-
-
4,500
-
159
Spike
-
21,344
45.94
-
Spike Level
-
16,667
-
-
64.62
-
% Recovery
-
101.06%
-
67.09%
Comments: TOC analysis conducted on dried (70 Ci, ground and sieved samples (120 mesh) using a Dohrmann DC-180 Carbon Analyzer.
Calibration, standardization and spiking are conducted following manufacturers directions using potassium hydrogen phthalate (KHP).
Samples are purged of inorganic carbon and dried at 70C before analysis. Values are expressed on a sediment dry weight basis (1040
fry multiplying the ppm C if the 70C dried sample by the ratio of % Solids 104C to % Solids 70C.
Ammonia was determined on KCI extracts following Plumb(1981). Sulfide analysis followed PSEP protocol modified to enhance sulfide
evolution, using the methylene blue colorimetric finish.
-------
CARR INLET EPA-3-57
SEDIMENT GRAIN SIZE DISTRIBUTION
Sample 10 B013A
CARR 4
Sieve Size -->
Finer than Phi Size -->
Gram Size ¦¦>
64 % SOLIDS
No. 4;
No 10:
No. 20:
No. 60: No. 140: No. 200: No. 230:
> 4750 4750-2000 2000-850 850-425
microns microns microns microns
425-250
microns
250-106
microns
106-75
microns
75-62 5
microns
4
62.5-31 2
microns
31.2-15 6
microns
6
156 7 8
microns
7
7 8-3 9
microns
8
3 9-19
microns
1 9-0.9
microns
10
<0 9
microns
Balance
Percent Passing - >
Fractional Percent % ->
100
0
100
0
100
0
99
1
97
2
81
16
62
19
52
10
23
29
11
12
Sample ID B013B
CARR 2
Sieve Size -->
Finer than Phi Size -->
Grain Size -->
No 4.
66 % SOLIDS
No 20:
No. 60: No. 140. No 200. No 230.
> 4750 4750-2000 2000-850 850 425
microns microns microns microns
425-250
microns
250-106
microns
106-75
microns
75-62 5
microns
62 5-31 2
microns
31 2-15 6
microns
b
156 7 8
microns
7
7.8-3 9
microns
3 9-1.9
microns
9
1.9-0.9
microns
10
<0.9
microns
Balance
Percent Passing
Fractional Percent %
100
0
100
0
100
0
99
1
96
3
63
33
40
23
31
9
18
13
12
6
10
2
Sample iD B013C
CARR 6
Sieve Size - >
Finer than Phi Size -->
Grain Size ->
No 4
33 % SOLIDS
No. 10:
No. 20.
No 40. No 60: No. 140: No. 200: No 230.
> 4750 4750 2000 2000 850 850-425
microns microns microns microns
425-250
microns
250-106
microns
106-75
microns
75-62 5
microns
62 5-31 2
microns
5
312-15 6
microns
6
156 7 8
microns
7
7 8-3 9
microns
8
3.9-1.9
microns
9
1.9-0.9
microns
10
<0 9
microns
Balance
Percent Passing (%) -
fractional Percent %
100
0
100
0
100
0
99
1
98
1
94
4
91
3
71
17
47
24
34
13
24
10
18
6
13
5
1
12
Sample iD. B013D
CARR A
Sieve Size >
Finer than Phi Size -->
Grain Size >
No 4.
83 % SOLiDS
No 10:
No 20
.4750 4750-2000 2000-850
No 4C:
650-425
microns
No 60: No 140: No. 200: No 230
425-250
microns
250-106
microns
106-75
microns
75-62 5
microns
62 5-31 2
microns
5
31.2-15 6
microns
6
15 6 7 8
microns
7
7 8-3.9
microns
3 9-1 9
microns
9
1 9-0 9
microns
10
<0 9
microns
Balance
Percent Passing
Fractional Percent %
92
18
72
10
63
9
42
21
22
20
11
11
SOIL TECHNOLOGY INC
J-250
PAGE 1
-------
18706 North Cieek Parkway Suite 110
Bottiell Washington 98011
206/485-5800 • FAX 485 5566
MASTER SAMPLE LOG AND
CHAIN OF CUSTODY RECORD
Project No
Project Name: _
Project Location:
Client Name:
3-5?-
CAKE. INLEf
£PA
Project Mgr:
"TVi V**ps. en
Sampling
Location
tmr~.
>wwer
CA«.
CAjS^T
Sample
Number
cake l>
Sample
Depth
Sample
Matrix
sewna/r
c
ii
Sampling
Date
Sampling
Time
112#
Sample
Collector(s)
Ti
H
II
Analyses
14
1
*-
tn
->
t/i
dU
n
$
A
•j
o
h*1
Sample Containers/
Preservatives
T
i
o
-y
Shipment
No.
Shipping
Container No.:
Custody
Seal No
Date
Shipped
Carrier.
Shipper:
Shipper
Bill No :
Comments
RELINQUISHED BY:
Signature . _
Date/Time.
Affiliation: _
RECEIVED BY
Signature
'il'iV-
Date/Time
Affiliation:
RELINQUISHED BY
Signature:
Date/Time
Affiliation:
RECEIVED BY
Signature:
Date/Time.
Affiliation:
• White Lab Returns to Originator Upon Receipt of Samples; • Canary Lab Retains: • Pink Lab Returns to Protect Manager with Final Report; * GokJenrod Retained by Sampler
-------
PHASE IIIA OYSTER
An Employee-Owned Company
-------
LARVAL COUNTS
An Employee-Owned Company
-------
Bioassay Organism Larval Counts - Oyster Phase lila
Replicate %
% Mortality and Mortality and
Sample Normal Abnormal Abnormality Abnormality
T=0 (800mL) 160
T=0 (800mL) 133
T=0 (800mL) 110
T=0 (800mL) 107
T=0 (800mL) 89
T=0 (BOOmL) 98
T=0 (800mL) 100
T=0(800mL) 164
T=0 (800mL) 102
T=0 (800mL) 188 mean= 125
T=0 (600mL) 160
T=0 (600mL) 142
T=0 (600mL) 199
T=0 (600m L) 184
T=0 (600mL) 214
T=0 (600mL) 197
T=0(600mL) 196
T=0 (600mL) 272
T=0 (600mL) 288
T=0 (600m L) 733 mean= 206
Sample % Mean Mean
Mortality and Standard Replicate % Sample* Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
-------
Bioassay Organism Larval Counts - Oyster (4 Hour Aerated)
Replicate %
% Mortality and Mortality and
Sample Normal Abnormal Abnormality Abnormality
SW 1a
49
1b
46
SW 2a
102
2b
85
SW 3a
102
3b
119
SW 4a
93
4b
81
SW 5a
62
5b
76
<30 la
60
1b
67
<30 2a
63
2b
62
<30 3a
88
3b
81
<30 4a
66
4b
57
<30 5a
57
5b
99
45-60 1a
46
1b
37
45-60 2a
106
2b
116
45-60 3a
102
3b
92
45-60 4a
41
4b
55
45-60 5a
54
5b
76
65-751a
88
lb
81
65-75 2a
56
2b
55
65-75 3a
70
3b
69
65-75 4a
44
4b
54
65-755a
81
5b
78
75-85 la
18
0
0.6
1
0.6
0.6
0
0.2
1
03
0.2
1
0.2
2
00
0.1
1
0.2
0
04
0 3
0
05
1
0.4
0.4
3
03
2
0.2
0 2
0
0.2
1
0.2
0.2
3
-0.1
0
0.0
-0.0
0
0.2
4
0.3
02
4
0.3
1
-0.2
0.0
1
04
1
0.5
05
3
-0.3
1
-0.4
-04
1
-0.3
1
-01
-0 2
2
0.5
1
0.3
0 4
1
0.3
1
0.1
0.2
0
-0.1
2
0.0
-0.0
1
03
0
0.3
03
1
0.1
0
02
0.1
2
0.5
1
0.3
04
2
00
0
0.0
00
22
08
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
0 3
81 5
0.2
0.1
0.1
01
03
0.2
0.2
0.0
0.0
0.0
0.0
00
0.0
00
00
00
0.0
0.0
0.0
0.0
0.0
00
0.0
0.0
0.1
01
0.0
00
0.0
0.0
0.0
0.0
0.0
00
0.0
00
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
00
06
0.0
0.0
00
0 0
0 0
0.0
0.0
0.0
0.0
0.0
00
0.0
0.0
0.0
0.0
00
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
-------
1b
21
20
0.7
75-85 2a
3
11
1.0
2b
6
8
09
75-85 3a
11
33
0.9
3b
2
25
1.0
75-85 4a
0
17
1.0
4b
7
18
0.9
75-85 5a
20
28
0.8
5b
14
25
0.8
SAMPLE
SAMPLE % MORTALITY
AND ABNORMALITY
SW
03
<30
0.1
45-60
0.1
65-75
0.2
75-85
0.9
0.8
0.9
0.9
1.0
0.8 0.9
0.5
0.8
0.6
0.8
0.9
1.0
0.7
0.6
0.6
0.5
0.7
0.8
0.9
0.6 0.7 0.1
-------
Bioassay Organism Larval Counts - Oyster (24 Hour Unaerated)
Sample
Normal
Abnormal
% Mortality and
Abnormality
Replicate %
Mortality and
Abnormality
SW 1a
132
1
-0.1
lb
117
2
0.0
-00
SW 2a
119
0
0.0
2b
134
1
-0.1
-0.0
SW 3a
183
0
-05
3b
197
2
-0.6
-05
SW 4a
121
0
00
4b
136
1
-0.1
-00
SW 5a
104
1
02
5b
79
1
0.4
03
<30 1a
142
2
-0 1
lb
131
1
0.0
-0.0
<30 2a
139
0
-0.1
2b
149
1
-0.1
-0.1
<30 3a
140
0
-0.1
3b
134
1
-0.0
-00
<30 4a
131
1
0.0
4b
160
0
-02
-0 1
<30 5a
121
0
0.1
5b
112
0
02
0 1
45-60 1a
162
1
-0.2
lb
164
2
-0.2
-02
45-60 2a
141
0
-0.1
2b
159
0
-0.2
-0.1
45-60 3a
147
0
-01
3b
135
2
-00
-0.1
45-60 4a
139
0
-01
4b
135
1
-0.0
-0 0
45-60 5a
131
0
0.0
5b
142
1
-0.1
-00
65-75 1a
131
1
0.0
1b
151
0
-01
-0 1
65-75 2a
151
2
-0.1
2b
147
0
-0.1
-0 1
65-75 3a
113
Q
0.1
3b
106
0
0.2
0.2
65-75 4a
165
1
-0.2
4b
143
0
-0.1
-0.2
65-75 5a
107
0
0.2
5b
133
1
-0.0
0 1
75-85 la
68
10
0 5
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
-0.1
132.2
0.3
-0.0
0.1
-0.1
0.1
-00
01
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
00
00
0.0
0.0
00
0.0
0.0
0.0
0.0
00
00
0.0
00
0.0
0.0
00
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
00
0.0
01
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
00
0.0
0.0
00
0.0
00
00
00
0.0
00
0.0
0.0
00
0.0
0.0
0.0
0.0
0.0
-------
1b
78
11
0.4
75-85 2a
74
3
0.4
2b
77
6
0.4
75-85 3a
77
33
0.4
3b
93
3
0.3
75-85 4a
76
17
0.4
4b
57
35
0.6
75-85 5a
106
23
0.2
5b
94
19
0.3
SAMPLE
SAMPLE % MORTALITY
AND ABNORMALITY
SW
-0.1
<30
-00
45-60
-01
65-75
-0.0
75-85
0.4
0.4
0.4
0.4
0.5
0.1
0.0
0.1
0.3
0.0
0.2
0.4
02
0.2
0.1
0.1
0.2
03
-------
Bioassay Organism Larval Counts - Oyster (4 Hour Unaerated)
Replicate %
% Mortality and Mortality and
Sample Normal Abnormal Abnormality Abnormality
SW 1a
152
0
-0.2
1b
138
0
-0.1
-0.2
SW 2a
153
0
-0 2
2b
158
3
-0.3
-0.3
SW 3a
147
0
-0.2
3b
153
4
-03
-0.2
SW 4a
140
0
-0.1
4b
134
0
-0.1
-01
SW 5a
142
0
-0 1
5b
138
1
-0 1
-0 1
<30 1a
102
0
0.3
1b
102
1
03
0.3
<30 2a
130
3
0.1
2b
124
3
0 1
0 1
<30 3a
122
3
0.2
3b
105
0
03
0.2
<30 4a
159
3
-0 1
4b
137
0
0.1
-0.0
<30 5a
136
4
0.1
5b
121
4
0.2
0.1
45-601a
138
2
0.1
1b
118
2
0.2
0.1
45-60 2a
144
3
00
2b
133
0
01
0.0
45-60 3a
134
2
0.1
3b
119
0
02
0.1
45-60 4a
124
2
0.1
4b
127
1
0.1
0.1
45-60 5a
135
2
01
5b
141
0
00
0.1
65-75 la
123
0
0.2
lb
115
1
0.2
0.2
65-75 2a
107
1
0.3
2b
94
1
04
0.3
65-75 3a
115
2
02
3b
110
1
02
02
65-75 4a
117
0
02
4b
112
1
0.2
0.2
65-75 5a
131
1
01
5b
133
0
0.1
0.1
75-85 la
87
10
0.4
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
0.0
0.0 0.0
0.0
0.0 0.0
0.0
00 00
0.0
00 0.0
145 5 0.0
-02 01 00 00 0.0 0.0
00
0.0 0.0
0.0
0.0 0.0
0.0
00 0.0
0.0
0.0 0.0
0.0
0.1 0.1 00 0.0 0.0 0.0
0.0
0.0 0.0
0.0
0.0 0.0
0.0
0.0 0.0
0.0
0.0 0.0
0.0
0.1 0.0 0.0 0.0 0.0 0.0
0.0
0.0 0.0
0.0
0.0 00
0.0
0.0 0.0
0.0
0.0 0.0
00
0 2 0.1 0.0 0 0 0.0 0.0
0.1
-------
1b
82
8
0.4
75-85 2a
46
8
0.7
2b
49
6
0.7
75-85 3a
51
4
06
3b
55
2
0.6
75-85 4a
96
9
0.3
4b
107
11
0.3
75-85 5a
106
6
03
5b
92
8
04
SAMPLE SAMPLE % MORTALITY
AND ABNORMALITY
SW -02
<30 01
45-60 0.1
65-75 0 2
75-85 0 5
0.4
0.7
0.6
0.3
0.3 0.5
01
0.1
0.1
0.1
0.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
01
0.1 0.1 0.0
-------
Bioassay Organism Larval Counts - Oyster (Greenbook July 15,1992)
Sample
Normal
Abnormal
% Mortality and
Abnormality
Replicate %
Mortality and
Abnormality
SW la
170
0
0.2
lb
179
1
0.1
0.1
SW 2a
186
0
0.1
2b
156
0
0.2
0.2
SW 3a
211
0
-0.0
3b
200
0
0.0
0.0
SW 4a
176
2
0.1
4b
194
2
0.0
0.1
SW 5a
170
0
0.2
5b
176
1
0.1
02
<301a
20
1.0
1b
0
0
1.0
1.0
<30 2a
1
42
1.0
2b
11
10
1.0
<30 3a
0
0
1.0
3b
0
0
1.0
10
<30 4a
0
0
10
4b
0
0
10
1.0
<30 5a
0
0
1.0
5b
0
0
1.0
1.0
45-601a
0
0
1.0
1b
0
0
1.0
10
45-60 2a
0
0
1.0
2b
0
0
1.0
1.0
45-60 3a
44
70
0.8
3b
112
80
0.4
06
45-60 4a
54
24
07
4b
8
4
10
0.8
45-60 5a
20
1.0
5b
5
54
1.0
10
65-75 la
7
119
1.0
1b
6
95
10
10
65-75 2a
37
1.0
2b
10
52
09
1.0
65-75 3a
84
88
0.5
3b
88
75
05
0.5
65-75 4a
40
160
08
4b
42
182
08
0.8
65-75 5a
32
75
08
5b
31
10
0.9
75-85 la
57
1.0
Sample %
Mortality and Standard
Abnormality Deviation
% Abnormality
Mean Mean
Replicate % Sample % Standard
Abnormality Abnormality Deviation
0.0
0.0 0.0
00
0.0 0.0
00
0.0 0.0
0.0
0.0 0.0
1818 0.0
0.1 0.1 0.0 0.0
1.0
1.0
1.0
1.0 1.0
1.0 0.0
06
04
0.3
03
10
0.9 0.2 0.9
0.9
0.9
1.0
0.8
0.5
05
08
0.8
07
0.6 0.2 1.0
1.0
0.5
03
1.0
0.9
09
0.5
08
09
0.0 0.0
1.0 0.0
0.6 0.3
0.8 0.2
-------
1b
87 *
1.0
75-85 2a
62 *
1.0
2b
93
1.0
75-85 3a
155 *
1.0
3b
122 *
1.0
75-85 4a
101
1.0
4b
104
1.0
75-85 5a
1 107
1.0
5b
1 117
1.0
* Noodefinitrve multicellular objects Best professional
judgement exercised, were scored as abnormal larvae
SAMPLE SAMPLE % MORTALITY
AND ABNORMALITY
SW 0 1
<30 10
45-60 0.9
65-75 0.8
75-85 10
1.0
1.0
1.0
1.0
1.0
1.0
0.0
1.0
1.0
10
10
1.0
1.0
10
1.0
1.0
1.0
1.0
1.0
1.0
1.0
-------
Bioassay Organism Larval Counts - Oyster (PSDDA July 15,1992)
Sample
Normal
Abnormal
Replicate %
% Mortality and Mortality and
Abnormality Abnormality
SW 1a
1b
SW 2a
2b
SW 3a
3b
SW 4a
4b
SW 5a
5b
<30 1a
1b
<30 2a
2b
<30 3a
3b
<30 4a
4b
<30 5a
5b
45-601a
1b
45-60 2a
2b
45-60 3a
3b
45-60 4a
4b
45-60 5a
5b
65-75 1a
1b
65-75 2a
2b
65-75 3a
3b
65-75 4a
4b
65-75 5a
5b
75-65 la
Probable triple innoc
Data discarded
179
183
164
178
82
102
127
154
21
22
4
3
5
4
14
16
2
2
5
6
10
1
10
7
8
8
10
16
39
27
8
6
9
6
4
1
5
6
11
2
6
2
1
1
0
0
0
0
0
2
2
4
0
0
0
0
0
4
0
0
2
2
1
0
1
0
0
0
0
3
01
0.1
0.2
0.1
0.6
05
04
0.2
0.9
0.8
1.0
1.0
1.0
1.0
0.9
0.9
1.0
1.0
1.0
1.0
0.9
10
0.9
1.0
0.9
0.9
0.9
09
07
08
0.9
1.0
0.9
10
1.0
1.0
10
1.0
0.9
0.1
02
06
0 3
0.9
10
1.0
0.9
1.0
1.0
1.0
0.9
0.9
0.9
0.8
1.0
09
1.0
1.0
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
03
146.125
0.2
0.9
01
0.9
0.0
0.9
0.1
0.0
0.0
0.0
0.0
0.0
0.0
00
00
0.1
0.2
0.3
0.3
0.2
0.0
0.0
0.0
0.0
0.0
0.3
0.3
0.3
0.0
0.0
0.0
0.0
00
0.3
0.0
0.0
0.1
0.2
0.1
0.0
0.1
0.0
0.0
0.0
0.0
0.2
0.0
0.0
00
0.0
0.2
0.3
0.1
0.0
0.0
03
01
0.0
0.0
0.1
0.0
0.2
0.1
0.0
0.0
0.0
0.0
0.1
0.1
0.1
0.1
0.1
0.1
-------
lb
0
1
10
75-85 2a
6
0
1.0
2b
2
0
1.0
75-85 3a
0
0
1.0
3b
0
0
1.0
75-85 4a
0
0
1.0
4b
0
0
1.0
75-85 5a
1
0
10
5b
0
0
1.0
SAMPLE SAMPLE % MORTALITY
AND ABNORMALITY
SW 0.3
<30 0.9
45-60 0.9
65-75 0 9
75-85 1.0
1.0
1.0
1.0
1.0
1.0
1.0
0.0
0.0
0.0
0.6
0.0
1.0
0.0
0.0
0.2
0.3
-------
Bjoassay Organism Larval Counts - Oyster (Cadmium Reference Test July 21,1992)
Replicate %
Sample %
Mean
Mean
% Mortality and
Mortality and
Mortality and
Standard
Replicate %
Sample %
Standard
Sample
Normal
Abnormal
Abnormality
Abnormality
Abnormality
Deviation
% Abnormality
Abnormality
Abnormality
Deviation
SW 1a
128
0
-0.0
0.0
lb
140
1
-0.1
-0.1
0.0
0.0
SW 2a
115
0
0.1
0.0
2b
114
1
0 1
0.1
0.0
0.1
0.0
0.0
0.0
0.0
0.75 1a
121
8
-0.0
0.1
0.75 1b
113
7
0.0
0.0
0.1
0.1
0.752a
96
5
0.2
00
0.75 2b
107
2
0 1
0.2
0.1
0.1
0.0
0.0
0.0
0.0
1.51a
43
20
0.5
0.3
1.51b
53
13
0.5
05
0.2
0.3
1.5 2a
47
10
0.5
0.2
1.5 2b
44
10
0.6
06
0.5
0.0
0.2
0.2
0.2
0.0
3.01a
4
20
0.8
0.8
3.0 1b
0
10
0.9
09
1.0
0.9
3.0 2a
11
23
0.7
0.7
3 0 2b
5
27
07
07
0.8
0.1
0.8
0.8
0.8
01
6.01a
6
8
09
0.6
6.01b
4
12
0.9
0.9
0.8
0.7
6.0 2a
6
13
0.8
0.7
6.02b
DUMPED BEFORE READING
0.8
0.9
0.0
0.7
0.7
0.0
-------
AMMONIA DATA
An Employee-Owned Company
-------
Exper Num
Phase IIIA Oyster: Calculation of Total and Unionized Ammonia - Orion Probe Data
920705 DATE 9-Jul-92 DAY Filename 0705cg0
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
81.1
24.8
-56.3
1
9.26
before samples
79.3
23.6
-55.7
-56.00
2
9.27
-56.18
3
9.28
(assumes pressure of
1 atm.l
79.3
22.7
-56.6
4
9.29
after samples
78.8
22.7
-56.1
-56.35
5
6
9.30
9.32
7
9.33
8
9.34
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution
NH3
(unionized)
ID
read
diluted
added
mv
added
w Std
ppm
C.
ppt
PH
factor
ppm
NH3 ppm
NSW Control
5.0
165.8
above
cut off
20.4
28
8.09
1.00
N.D.
N.D.
75-85% 4hr A psdda
5.0
132.4
20
124.6
50
20.2
30
8.12
1.00
0.52
0.02
75-85% 4hr UA PSDDA
5.0
136.9
above
cut off
21.2
29
7.90
1.00
N.D.
N.D.
75-85% 24hr UA PSDDA
5.0
133.9
20
124.2
50
20.9
7.89
1.00
0.40
0.01
75-85% El. G.B.
5.0
76.3
380
61.5
50
20.3
28
7.95
1.00
3.90
0.12
65-75% 4 hr A PSDDA
5.0
137.2
above
cut off
20.1
29
8.12
1.00
N.D.
N.D.
65-75% 4hr UA PSDDA
5.0
142.4
above
cut off
21.1
29
7.88
1.00
N.D.
N.D.
65-75% 24hr UA PSDDA
5.0
141.8
above
cut off
20.9
29
7.83
1.00
N.D.
N.D.
65-75% El. G.B.
5.0
96.6
150
82.1
50
20.3
29
8.13
1.00
1.73
0.08
45-60% 4hr A PSDDA
5.0
132.5
20
125.1
50
20.2
29
8.09
1.00
0.56
0.02
45-60% 4Hr UA PSDDA
5.0
132.3
20
124.0
50
21.2
28
7.85
1.00
0.49
0.01
45-60% 24hr UA PSDDA
5.0
136.1
above
cut off
21.0
29
7.83
1.00
N.D.
N.D.
45-60% El. G.B.
5.0
72.4
460
57.9
50
20.5
28
8.24
1.00
4.70
0.27
<30% 4hr A PSDDA
5.0
144.4
above
cut off
20.1
30
8.08
1.00
N.D,
N.D.
<30% 4hr UA PSDDA
5.0
144.1
above
cut off
21.0
29
7.88
1.00
N.D.
N.D.
<30% 24hr UA PSDDA
5.0
143.4
above
cut off
21.3
29
7.86
1.00
N.D.
N.D.
<30% El.G.B.
5.0
93.1
170
79.6
50
20.5
29
8.14
1.00
2.13
0.10
NSW 24 hr
5.0
168.8
above
cut offf
20.8
28
8.05
1.00
N.D.
N.D.
N.D.= Non detection. Value was below method detection limit.
-------
Exper Num
Phase IIIA Oyster: Calculation of Total and Unionized Ammonia - Orion Probe Data
920705 DATE 13-Jul-92 DAY Filename 0705CG48
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
85.4
28.6
-56.8
1
9.26
before samples
83.4
27.6
-55.8
-56.43
2
9.27
84.6
27.9
-56.7
-56.12
3
9.28
(assumes pressure of
1 atm.)
84.3
29.1
-55.2
4
9.29
after samples
83.8
83.1
27.5
27.2
-56.3
-55.9
-55.80
5
6
9.30
9.32
7
9.33
8
9.34
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added
w Std
ppm
C.
ppt
PH
factor
ppm
NH3 ppm
NSW Control
5.0
143.3*
50
136.2
50
20.6
28
8.09
1.00
N.D.
N.D.
75-85% 4hr A PSDDA
5.0
143.2
50
123.4
50
20.3
28
8.12
1.00
0.39
0.02
75-85% 4hr UA PSDDA
5.0
142.2
50
124.5
50
21.3
29
7.90
1.00
0.46
0.01
75-85% 24 hr UA PSDDA
5.0
141.1
50
122.6
50
21.2
28
7.89
1.00
0.43
0.01
75-85% El. G.B.
5.0
83.6
260
72.3
50
21.0
28
7.95
1.00
3.87
0.12
65-75% 4hr A PSDDA
5.0
146.5*
50
135.1
50
20.4
28
8.12
1.00
N.D.
N.D.
65-75% 4hr UA PSDDA
5.0
145.5*
50
134.0
50
21.3
29
7.88
1.00
N.D.
N.D.
65-75% 24hr UA PSDDA
5.0
146.8*
50
135.6
50
21.1
29
7.83
1.00
N.D.
N.D.
65-75% El. G.B.
5.0
113.3
60
104.5
50
20.5
29
8.13
1.00
1.33
0.06
45-60% 4hr A PSDDA
5.0
144.5*
50
130.8
50
20.4
28
8.09
1.00
N.D.
N.D.
45-60% 4hr UA PSDDA
5.0
149.1
50
127.8
50
21.3
28
7.85
1.00
0.35
0.01
45-60% 24hr UA PSDDA
5.0
148.6
50
129.0
50
21.3
28
7.83
1.00
0.40
0.01
45-60% El. G.B.
5.0
82.8
290
70.7
50
20.6
28
8.24
1.00
3.93
0.22
<30% 4hr A PSDDA
5.0
147.1 *
50
135.4
50
20.4
28
8.08
1.00
N.D.
N.D.
<30% 4hr UA PSDDA
5.0
136*
50
130.6
50
21.9
28
7.88
1.00
N.D.
N.D.
<30% 24hr UA PSDDA
5.0
149.9
at
cut off
21.5
28
7.86
1.00
N.D.
N.D.
<30% El.G.B.
5.0
124.0
80
108.3
50
20.0
28
8.14
1.00
0.86
0.04
NSW 24hr
5.0
135.7*
50
130.0
50
21.1
28
8.05
1.00
N.D.
N.D.
"Did not put 50ppm std. in until the mv's rose about 2.0-5.0mv digits.
They rose quickly.
N.D. = Non detection. Value was below method detection limit.
-------
PHYSICAL MONITORING DATA
An Employee-Owned Company
-------
PHASE fflA BIVALVE BIOASSAY PHYSICAL MONITORING DATA
Client EPA TESC 3-57
Experiment # 92070 S E 92070 6 (CD)
Day 0 Day 1 Day 2
Date 8-Jul-92 9-Jul-92 10-Jul-92
Time
Observer M. Pelletier, C. Shochan, C. Sheehan, M. Pelltier J. Dinsdalc, M. Pelltier,
J. Dinsdale
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
< 30 4-UA
21
29
5.7
7.88
22.0
29
5.1
21.9
28
5.4
7.88
45-60 4-UA
21.2
28
5.8
7.85
21.8
30
5.1
21.3
28
4.7
; 7.85
65-75 4-UA
21.1
29
6.2
7.88
21.8
30
5.3
21.3
29
5.0
7.88
75-85 4-UA
21.2
29
6
7.9
21.5
30
4.7
21.3
29
4.8
7.9
SW 4-UA
20.6
28
7.4
8.09
21.7
29
6.8
21.1
28
7.0
8.09
<30 24-UA
21.3
29
5.0
7.86
21.7
30
5.0
21.5
28
4.8
7.86
45-65 24-UA
21
29
5.2
7.83
21.4
29
5.0
21.3
28
4.7
7.83
65-75 24-UA
20.9
29
5.5
7.83
21.4
29
5.1
21.1
29
5.1
7.83
75-85 24-UA
20.9
Not Taken
4.7
7.89
21.3
29
5.1
21.2
28
5.3
7.89
SW 24-UA
20.8
28
7.0
8.05
21.1
29
6.9
21.1
28
6.9
8.05
75-85 PSDDA
20.4
28
5.7
7.95
20.8
29
0.4
21.2
28
5.5
7.95
65-75 PSDDA
20.1
29
6.1
8.13
20.8
30
4.2
20.5
30
7.2
8.13
45-60 PSDDA
20.6
28
6.1
8.24
20.8
29
3.7
20.6
28
7.1
8.24
<30 PSDDA
20.4
28
2.4
8.14
20.8
29
3.2
20.5
28
7.0
8.14
SW PSDDA
20.3
28
7.1
N.D.
20.8
28
7.1
20.5
28
7.2
N.D.
Cd Lo 0.75
20.8
29
7.3
N.D.
21.0
30
6.9
20.9
28
6.8
N.D.
Cd Hi 6.0
20.2
30
7.5
N.D.
20.6
30
7.0
20.6
28
7.0
N.D.
75-85 GB
20.3
30
5.6
7.95
21.0
29
0.4
20.8
28
6.7
7.95
65-75 GB
20.6
28
6.3
8.13
20.9
29
4.4
20.6
28
7.1
8.13
45-60 GB
20.5
28
6.1
8.24
21.2
28
4.1
20.6
28
7.1
8.24
<30 GB
20.5
29
3.5
8.14
21.1
29
3.7
20.5
28
7.0
8.14
SW GB
20.5
28
7.2
N.D.
21.2
28
6.9
20.6
28
7.2
N.D.
SW 4-A
20.1
28
7.3
8.09
20.6
29
7.2
20.3
28
7.1
8.09
75-85 4A
20.2
30
7.0
8.12
20.6
30
7.3
20.3
28
7.3
8.12
65-75 4A
20.1
29
7.3
8.12
20.5
30
7.3
20.4
28
7.2
8.12
45-60 4A
20.2
29
7.2
8.09
20.5
30
7.2
20.4
28
7.2
8.09
<30 4A
20.1
30
7.2
8.08
20.5
29
7.2
20.4
28
7.2
8.08
-------
REFERENCE TOXICANT
An Employee-Owned Company
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
[IIAOYS
Observed
Adjusted
Predicted
Number
Number
Proportion
Proportion
Proportion
Cone.
Exposed
Resp.
Responding
Responding
Responding
Control
100
1
0.0100
0.0000
0.0086
0.7500
100
8
0.0800
0.0720
0.1048
1.5000
100
52
0.5200
0.5158
0.4412
3.0000
100
80
0.8000
0.7983
0.8312
Chi - Square Heterogeneity = 4.038
***************************************************************
* WARNING *
* *
* Significant heterogeneity exists. The results reported *
* for this data set may not be valid. The results should *
* be interpreted with appropriate caution. *
****************************************************************
* NOTE *
* *
* Slope not significantly different from zero. *
* EC fiducial limits cannot be computed. *
***************************************************************
Mu = 0.216311
Sigma = 0.271959
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 4.204619 0.234776 ( 1.221560, 7.187678)
Slope 3.677029 0.775439 ( -6.175696, 13.529754)
Spontaneous 0.008604 0.018512 ( -0.226614, 0.243822)
Response Rate
-------
PHIIIAOYS
Estimated EC Values and Confidence Limits
Lower Upper
Point Cone. 95% Confidence Limits
EC 1.00
0.3834
EC 5.00
0.5874
EC10.00
0.7375
EC15.00
0.8599
EC50.00
1.6455
EC85.00
3.1490
EC90.00
3.6716
EC95.00
4.6095
EC99.00
7.0627
-------
PHIIIAOYS
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
. . . o
5+
4+
2+.
1+
—I 1 (. ^ 1
EC10 EC25 EC50 EC75 EC90 EC99
0+
_+
EC01
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PHIIIAEC
Cone.
Control
0.7500
1.5000
3.0000
Number
Exposed
100
100
100
100
Number
Resp.
1
5
22
84
Observed
Proportion
Responding
0.0100
0.0500
0.2200
0.8400
Adjusted
Proportion
Responding
0.0000
0.0256
0.2000
0.8359
Predicted
Proportion
Responding
0.0250
0.0055
0.2132
0.8300
Chi - Square Heterogeneity =
1.432
Mu
Sigma
0.312940
0.172046
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept 3.181066 0.252121 ( 2.686908, 3.675224)
Slope 5.812410 0.707945 ( 4.424838, 7.199983)
Spontaneous 0.025005 0.012173 ( 0.001147, 0.048864)
Response Rate
-------
PHIIIAEC
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
0.8179
0.5951
1.0003
EC 5.00
1.0714
0.8439
1.2504
EC10.00
1.2372
1.0148
1.4109
EC15.00
1.3634
1.1480
1.5326
EC50.00
2.0556
1.8777
2.2382
EC85.00
3.0992
2.7903
3.5979
EC90.00
3.4153
3.0351
4.0643
EC95.00
3.9440
3.4291
4.8815
EC99.00
5.1662
4.2918
6.9140
-------
PHIIIAEC
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4 +
-o
3 +
2 +
1+
. o,
0+
-+ + +
-r —
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
PHASE IIIA ECHINODERMS
An Employee-Owned Company
-------
LARVAL COUNTS
An Employee-Owned Company
-------
BIO ASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia
Replicate %
% Mortality and Mortality and
Sample Normal Abnormal Abnormality Abnormality
T=0 (800mL) 173
T=0 (800mL) 153
T=0 (800mL) 127
T=0 (800mL) 156
T=0 (800mL) 150
T =0 (800mL) 193
T=0 (800mL) 110
T=0 (800mL) 131
T=0 (800mL) 141
T=0 (800mL) 138 mean= 147
T=0 (600mL) 185
T=0 (600mL) 186
T=0 (600mL) 202
T=0 (600mL) 207
T=0 (600mL) 148
T=0 (600mL) 191
T=0 (600mL) 206
T=0 (600mL) 173
T=0 (600mL) 228
T=0 (600mL) 188 mean= 191
Sample % Mean Mean
Mortality and Standard Replicate % Sample %
Abnormality Deviation % Abnormality Abnormality Abnormality
-------
BIOASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (4 Hour Aerated)
Replicate %
% Mortality and
Mortality and
Sample
Normal
Abnormal
Abnormality
Abnormality
SW la
106
17
16.4%
lb
104
13
20.5%
18.5%
SW 2a
130
22
-3.3%
2b
113
6
19.2%
7.9%
SW 3a
128
18
0.8%
3b
110
8
19.8%
10.3%
SW 4a
102
17
19.2%
4b
113
14
13.7%
16.4%
SW 5a
130
8
6.2%
5b
99
15
22.6%
14.4%
<30 la
56
7
50.7%
lb
50
6
55.9%
53.3%
<30 2a
144
25
-26.9%
2b
115
11
-1.3%
-14.1 %
<30 3a
116
13
-2.2%
3b
109
13
4.0%
0.9%
<30 4a
104
8
8.4%
4b
118
11
-4.0%
2.2%
<30 5a
99
7
12.8%
5b
113
10
0.4%
6.6%
45-60 la
101
19
11.0%
lb
68
21
40.1%
25.6%
45-60 2a
101
34
11.0%
2b
88
31
22.5%
16.7%
45-60 3a
105
26
7.5%
3b
89
28
21.6%
14.5%
45-60 4a
134
17
-18.1%
4b
114
21
-0.4%
-9.3%
45-60 5«
106
8
6.6%
5b
78
8
31.3%
18.9%
65-75 la
107
9
5.7%
lb
109
14
4.0%
4.8%
65-75 2a
118
21
-4.0%
2b
134
8
-18.1%
-11.0%
65-75 3a
110
10
3.1%
3b
121
13
-6.6%
-1.8%
65-75 4a
132
11
-16.3%
4b
125
11
-10.1%
-13.2%
65-75 5a
104
5
8.4%
5b
101
5
11.0%
9.7%
Sample %
Mortality and
Abnormality
Standard
Deviation
% Abnormality
Mean
Replicate %
Abnormality
Mean
Sample %
Abnormality
Standard
Deviation
x =
13.5%
113.5
3.9%
9.8%
22.9%
13.3%
11.9%
-2.3%
8.8%
13.8%
11.1%
14.5%
5.0%
12.3%
6.8%
14.3%
11.0%
5.8%
13.2%
11.1%
10.7%
14.8%
8.7%
10.1%
10.7%
7.1%
8.5%
6.6%
8.1%
15.8%
23.6%
25.2%
26.1%
19.8%
23.9%
11.3%
15.6%
7.0%
9.3%
7.8%
11.4%
15.1%
5.6%
8.3%
9.7%
7.7%
8.1%
4.6%
4.7%
12.5%
9.8%
9.6%
12.7%
9.5%
10.9%
11.8%
10.4%
7.8%
7.4%
19.7%
25.6%
21.9%
13.4%
8.2%
9.6%
10.4%
9.0%
7.9%
4.7%
10.8%
1.55
9.6%
1.7%
17.8%
6.2%
8.3%
2.0%
-------
BIO ASS AY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (4 Hour Aerated) (continued)
Sample Normal Abnormal
75-85 la
84
11
lb
110
15
75-85 2a
no
5
2b
101
13
75-85 3a
61
4
3b
98
6
75-85 4a
90
8
4b
99
8
75-85 5a
97
12
5b
94
9
Replicate % Simple %
% Mortality and Mortality and Mortality and
Abnormality Abnormality Abnormality
26.0%
3.1* 14.5%
3.1%
11.0% 7.0%
46.3%
13.7% 30.0%
20.7%
12.8% 16.7%
14.5%
17.2% 159% 16.8%
SAMPLE SAMPLE % MORTALITY
AND ABNORMALITY
SW 13.5%
<30 9.8%
45-60 13.3%
65-75 -2.3%
75-85 16.8%
Standard
Deviation
% Abnormality
Mean Mean
Replicate % Sample % Standard
Abnormality Abnormality Deviation
7.4%
11.6%
12.0%
4.3%
11.4%
6.2%
5.8%
8.2%
7.5%
11.0%
8.7%
11.8%
7.9%
6.0%
7.8%
9.9%
8.7%
2.0%
-------
BIOASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase HIa (24 Hour Unaerated)
Replitate % .
% Mortality and Mortality and
Sample
Normal
Abnormal
Abnormality
Abnormality
SW la
140
11
-2.6%
lb
110
7
20.5%
9.0%
SW 2a
130
8
6.2%
2b
124
23
0.1%
3.2%
SW 3a
97
4
31.4%
3b
113
6
19.2%
25.3%
SW 4a
101
17
19.8%
4b
100
5
28.7%
24.3%
SW 5a
124
8
10.3%
5b
127
10
6.9%
8.6%
<30 la
135
14
-15.8%
lb
146
15
-25.2%
-20.5%
<30 2a
138
11
-18.4%
2b
138
14
-18.4%
-18.4%
<30 3a
136
7
-16.6%
3b
137
16
-17.5%
-17.1%
<30 4a
121
5
-3.8%
4b
156
13
-33.8%
-18.8%
<30 5a
140
16
-20.1%
5b
164
6
-40.7%
-30.4%
45-60 la
176
5
-50.9%
lb
119
19
-2.1%
-26.5%
45-60 2a
136
13
-16.6%
2b
121
9
-3.8%
-10.2%
45-60 3a
101
6
13.4%
3b
123
8
-5.5%
3.9%
45-60 4a
112
11
3.9%
4b
131
6
-12.3%
-4.2%
45-60 5a
146
10
-25.2%
5b
122
10
-4.6%
-14.9%
65-75 la
113
7
3.1%
lb
107
4
8.2%
5.7%
65-75 2a
123
8
-5.5%
2b
131
11
-12.3%
-8.9%
65-75 3a
101
8
13.4%
3b
151
11
-29.5%
-8.1%
65-75 4a
119
11
-2.1%
4b
116
8
0.5%
-0.8%
65-75 5a
115
16
1.4%
5b
114
18
2.2%
1.8%
Simple % Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
7.3%
6.0% 6.6%
5.8%
15.6% 10.7%
4.0%
5.0% 4.5%
14.4%
4.8% 9.6%
x= 116.6 6.1%
14.1% 9.0% 7.3% 6.7% 7.6% 2.2%
9.4%
9.3% 9.4%
7.4%
9.2% 8.3%
4.9%
10.5% 7.7%
4.0%
7.7% 5.8%
10.3%
-21.0% 4.8% 3.5% 6.9% 7.6% 1.2%
2.8%
13.8% 8.3%
8.7%
6.9% 7.8%
5.6%
6.1% 5.9%
8.9%
4.4% 6.7%
6.4%
-10.4% 10.2% 7.6% 7.0% 7.1% 0.9%
5.8%
3.6% 4.7%
6.1%
7.7% 6.9%
7.3%
6.8% 7.1%
8.5%
6.5% 7.5%
12.2%
-2.1% 5.6% 13.6% 12.9% 7.8% 2.7%
-------
BIO ASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase III. (24 Hour Unaerated) (continued)
Sample Normal Abnormal
75-85 la
125
12
lb
165
14
75-85 2a
125
12
2b
130
17
75-85 3a
172
11
3b
154
14
75-85 4a
170
16
4b
167
10
75-85 5a
115
8
5b
121
11
SAMPLE
SAMPLE % MORTALITY
AND ABNORMALITY
SW
14.1%
<30
-21.0*
45-60
-10.4%
65-75
-2.1%
75-85 -23.8*
Replicate % Sample %
% Mortality and Mortality and Mortality and
Abnormality Abnormality Abnormality
-7.2*
-41.5* -24.4%
-7.2%
-11.5* -9.3*
-47.5*
-32.1* -39.8*
-45.8*
-43.2* -44.5*
1.4*
-3.8* -1.2* -23.8*
Mean
Standard Replicate % Sample * Standard
Deviation * Abnormality Abnormality Abnormality Deviation
8.8*
7.8* 8.3*
8.8*
11.6% 10.2%
6.0*
8.3* 7.2*
8.6*
5.6* 7.1%
6.5%
16.8% 8.3% 7.4% 8.0% 1.1%
-------
BIO ASSAY ORGANISM LARVAL COUNTS SAND DOLLAR Phase Ilia (4 Hour Unaerated)
Replicate %
% Mortality and
Mortality and
Sample
Normal
Abnormal
Abnormality
Abnormality
SW la
146
10
-6.0%
lb
139
13
-3.3%
-4.6%
SW 2a
112
8
18.5%
2b
118
7
15.1%
16.8%
SW 3a
118
12
11.7%
3b
115
8
16.4%
14.1%
SW 4a
102
7
26.0%
4b
122
14
7.6%
16.8%
SW 5a
106
7
23.2%
5b
106
4
25.3%
24.3%
<30 la
46
9
62.6%
lb
51
10
56.9%
. 59.8%
<30 2a
43
5
63.7%
2b
54
3
54.4%
59.0%
<30 3a
65
15
45.1%
3b
51
11
56.9%
51.0%
<30 4a
50
1
57.8%
4b
64
2
45.9%
51.9%
<30 5a
41
10
65.4%
5b
35
8
70.4%
67.9%
45-60 la
99
2
16.4%
lb
81
2
31.6%
24.0%
45-60 2a
63
12
46.8%
2b
96
10
18.9%
32.9%
45-60 3a
92
4
22.3%
3b
68
2
42.6%
32.4%
45-60 4a
87
21
26.5%
4b
90
20
24.0%
25.3%
45-60 5a
69
37
41.7%
5b
65
25
45.1%
43.4%
65-75 la
91
2
23.1%
lb
75
9
36.7%
29.9%
65-75 2a
71
4
40.0%
2b
61
9
48.5%
44.3%
65-75 3a
79
19
33.3%
3b
59
25
50.2%
41.7%
65-75 4a
78
8
34.1%
4b
74
13
37.5%
35.8%
65-75 5a
20
57
83.1%
5b
17
48
85.6%
84.4%
Sample %
Mortality and
Abnormality
Standard
Deviation
% Abnormality
Mean
Replicate %
Abnormality
Mean
Sample %
Abnormality
Standard
Deviation
x =
13.5%
118.4
9.7%
57.9%
6.1%
31.6%
6.9%
47.2%
19.2%
6.4%
8.6%
6.7%
5.6%
9.2%
6.5%
6.4%
10.3%
6.2%
3.6%
16.4%
16.4%
10.4%
5.3%
18.8%
17.7%
2.0%
3.0%
19.6%
18.6%
2.0%
2.4%
16.0%
9.4%
4.2%
2.9%
19.4%
18.2%
34.9%
27.8%
2.2%
10.7%
5.3%
12.9%
19.4%
29.8%
9.3%
14.9%
74.0%
73.8%
7.5%
6.1%
7.9%
8.4%
4.9%
16.4%
7.8%
18.2%
2.5%
19.1%
2.2%
12.7%
3.5%
18.8%
31.3%
6.4%
9.1%
24.6%
12.1%
73.9%
7.0%
1.3%
12.8%
6.5%
13.7%
10.7%
25.2%
25.1%
-------
BIO ASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia {4 Hour Unaerated) (continued)
Sample Normal Abnormal
75-85 la
109
8
lb
95
4
75-85 2a
68
12
2b
80
13
75-85 3«
67
12
3b
64
9
75-85 4a
62
16
4b
63
16
75-85 5a
24
28
5b
13
37
SAMPLE
SAMPLE % MORTALITY
AND ABNORMALITY
SW
13.5%
<30
57.9%
45-60
31.6%
65-75
47.2%
75-85 45.5*
Replicate % Sample %
% Mortality and Mortality and Mortality and
Abnormality Abnormality Abnormality
7.9%
19.8% 13.9%
42.6%
32.4% 37.5%
43.4%
45.9% 44.7%
47.6%
46.8% 47.2%
79.7%
89.0% 84.4% 45.5%
Mean Mean
Standard Replicate % Sample % Standard
Deviation % Abnormality Abnormality Abnormality Deviation
6.8%
4.0% 5.4%
15.0%
14.0% 14.5%
15.2%
12.3% 13.8%
20.5%
20.3% 20.4%
53.8%
22.7% 74.0% 63.9% 23.6% 20.7%
-------
BIOASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (Greenbook)
Replicate % "
Sample
Normal
Abnormal
% Mortality and
Abnormality
Mortality and
Abnormality
SW la
128
26
19.5%
lb
124
14
27.9%
23.7%
SW 2a
164
16
6.0%
2b
142
27
11.7%
8.8%
SW 3a
170
27
-2.9%
3b
165
23
1.8%
-0.6%
SW 4a
190
16
-7 6%
4b
181
10
0.2%
-3.7%
SW 5a
163
18
5.4%
5b
156
22
7.0%
6.2%
<30 la
0
*
100.0%
lb
0
*
100.0%
100.0%
<30 2a
0
*
100.0%
2b
0
*
100.0%
100.0%
<30 3a
27
*
100.0%
3b
40
*
100.0%
100.0%
<30 4a
12
*
100.0%
4b
44
*
100.0%
100.0%
<30 5a
13
*
100.0%
5b
10
*
100.0%
100.0%
45-60 la
92
*
100.0%
lb
88
*
100.0%
100.0%
45-60 2a
100.0%
2b
100.0%
100.0%
45-60 3a
100.0%
3b
100.0%
100.0%
45-60 4a
100.0%
4b
100.0%
100.0%
45-60 5a
100.0%
5b
100.0%
100.0%
65-75 la
25
*
100.0%
lb
111
*
100.0%
100.0%
65-75 2a
38
*
100.0%
2b
29
*
100.0%
100.0%
65-75 3a
0
82
100.0%
3b
0
99
100.0%
100.0%
65-75 4a
100.0%
4b
100.0%
100.0%
65-75 5a
100 0%
5b
100 0%
100.0%
Sample %
Mortality and
Abnormality
Standard
Deviation
% Abnormality
Mean
Replicate %
Abnormality
Mean
Sample %
Abnormality
Standard
Deviation
x =
6.9%
158.3
9.5%
16.9%
10.1%
8.9%
16.0%
13.7%
12.2%
7.8%
5.2%
9.9%
12.4%
13.5%
12.4%
13.0%
6.5%
11.2%
11.3%
2.5%
100.0%
0.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
0.0%
100.0%
0.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
0.0%
100.0% 0.0%
100.0%
0.0%
-------
BIOASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase HIa (Greenbook) (continued)
Replicate %
% Mortality and Mortality and
Sample Normal Abnormal Abnormality Abnormality
75-85 la 40 * 100.0%
lb 30 * 100.0% 100.0%
75-85 2a 100.0%
2b 100.0% 100.0%
75-85 3a 100.0%
3b 100.0% 100.0%
75-85 4a 100.0%
4b 100.0% 100.0%
75-85 5a 100.0%
5b 100.0% 100.0%
* Nondefimtive multicellular objects. Best professional
judgement was exercised, were scored as abnormal larvae.
SAMPLE SAMPLE % MORTALITY
AND ABNORMALITY
SW 6.9%
<30 100.0%
45-60 100.0%
65-75 100.0%
75-85 100.0%
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
100.0%
100.0% 100.0%
100.0%
0.0%
100.0%
0.0%
-------
BIOASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (PSDDA)
Replicate %
Sample
Normal
Abnormal
% Mortality and
Abnormality
Mortality and
Abnormality
SW la
136
17
20.1%
lb
119
18
28.4%
24.2%
SW 2a
137
37
9.1%
2b
119
25
24.8%
16.9%
SW 3a
107
33
26.9%
3b
107
30
28.4%
27.6%
SW 4a
148
20
12.2%
4b
131
17
22.7%
17.5%
SW 5a
175
3
7.0%
5b
147
8
19.0%
13.0%
<30 la
1
1
99.2%
lb
3
3
97.7%
98.5%
<30 2a
10
3
92.5%
2b
10
3
92.5%
92.5%
<30 3a
16
3
87.9%
3b
0
4
100.0%
94.0%
<30 4a
0
3
100.0%
4b
0
1
100.0%
100.0%
<30 5a
0
3
100.0%
5b
1
1
99.2%
99.6%
45-60 la
6
0
95.5%
lb
1
0
99.2%
97.4%
45-60 2a
13
1
90.2%
2b
13
2
90.2%
90.2%
45-60 3a
9
1
93.2%
3b
9
0
93.2%
93.2%
45-60 4a
14
2
89.4%
4b
15
1
88.7%
89.1%
45-60 5a
9
5
93.2%
5b
9
1
93.2%
93.2%
65-75 la
3
1
97.7%
lb
6
2
95.5%
96.6%
65-75 2a
4
0
97.0%
2b
0
0
100.0%
98.5%
65-75 3a
2
0
98.5%
3b
6
1
95.5%
97.0%
65-75 4a
12
1
91.0%
4b
11
0
91.7%
91.3%
65-75 5a
12
2
91.0%
5b
15
0
88.7%
89.8%
Sample %
Mortality and
Abnormality
Standard
Deviation
% Abnormality
Mean Mean
Replicate % Sample % Standard
Abnormality Abnormality Deviation
19.9%
132.6
5.3%
96.9%
3.1%
92.6%
2.9%
94.6%
3.4%
11.1%
13.1%
21.3%
17.4%
23.6%
21.9%
11.9%
11.5%
1.7%
5.2%
50.0%
50.0%
23.1%
23.1 %
15.8%
100.0%
100.0%
100.0%
100.0%
50.0%
0.0%
0.0%
7.1%
13.3%
10.0%
0.0%
12.5%
6.3%
35.7%
10.0%
25.0%
25.0%
0.0%
0.0%
14.3%
7.7%
0.0%
14.3%
0.0%
12.1%
19.3%
22.7%
11.7%
3.4%
50.0%
23.1%
57.9%
100.0%
75.0%
0.0%
10.2%
5.0%
9.4%
22.9%
25.0%
0.0%
7.1%
3.8%
7.1%
13.9%
6.7%
61.2%
25.6%
9.5%
7.6%
8.6%
8.6%
-------
BIO ASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (PSDDA) (continued)
Replicate %
% Mortality and
Mortality and
Sample
Normal
Abnormal
Abnormality
Abnormality
75-85 la
0
2
100.0%
lb
0
3
100.0%
100.0%
75-85 2a
0
3
100.0%
2b
0
3
100.0%
100.0%
75-85 3a
0
8
100.0%
3b
0
6
100.0%
100.0%
75-85 4a
0
2
100.0%
4b
0
7
100.0%
100.0%
75-85 5a
0
4
100.0%
5b
0
2
100.0%
100.0%
SAMPLE
SAMPLE % MORTALITY
AND ABNORMALITY
SW
19.9%
<30
96.9%
45-60
92 6%
65-75
94.6%
75-85 100.0%
Sample % Mean Mean
Mortality and Standard Replicate % Sample % Standard
Abnormality Deviation % Abnormality Abnormality Abnormality Deviation
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
100.0% 0.0% 100.0%
100.0%
100.0%
100.0%
100.0%
100.0% 100.0% 0.0%
-------
BIO ASSAY ORGANISM LARVAL COUNTS - SAND DOLLAR Phase Ilia (Cadmium Reference Test)
Replicate %
Sample %
Mean
Mean
% Mortality and
Mortality and
Mortality and
Standard
Replicate %
Sample %
Standard
Sample
Normal
Abnormal
Abnormality
Abnormality
Abnormality
Deviation
% Abnormality
Abnormality
Abnormality
Deviation
SW la
120
10
11.7%
7.7%
lb
98
4
30,7%
21.2%
3.9%
5.8%
SW 2a
124
15
5.6%
10.8%
2b
112
14
14.4%
10.0%
15.6%
5.6%
11.1%
11.0%
8.4%
2.6%
1.5 la
125
20
1.5%
13.8%
1.5 lb
95
17
23.9%
12.7%
15.2%
14.5%
1.5 2a
92
16
26.6%
14.8%
1.5 2b
106
17
16.4%
21.5%
17.1%
4.4%
13.8%
14.3%
14.4%
0.1 %
3.0 la
94
32
14.4%
25.4%
3.0 lb
103
58
-9.4%
2.5%
36.0%
30.7%
3 .0 2a
93
42
8.3%
31.1%
3.0 2b
105
35
4.9%
6.6%
4.6%
2.0%
25.0%
28.1%
29.4%
1.3%
6.0 la
6
103
26.0%
94.5%
6.0 lb
15
103
19.8%
22.9%
87.3%
90.9%
6.0 2a
3
118
17.8%
97.5%
6.0 2b
12
114
14.4%
16.1%
19.5%
3.4%
90.5%
94.0%
92.4%
1.6%
12.0 la
0
145
1.5%
100.0%
12.0 lb
0
153
-3.9%
-1.2%
100.0%
100.0%
12.0 2a
0
172
-16.8%
100.0%
12.0 2c
0
147
0.1%
8.4%
-4.8%
3.6%
100.0%
100.0%
100.0%
0.0%
* vial 12.0 2b poured into broken dish, not counted
-------
AMMONIA DATA
An Employee-Owned Company
-------
Exper Num
Phase IIIA: Calculation of Total and Unionized Ammonia - Orion Probe Data
920704 DATE 9-Jul-92 DAY Filename 0704DEO
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
83.8
27.2
-56.6
1
9.26
before samples
81.6
26.0
-55.6
-56.10
2
9.27
-55.98
3
9.28
(assumes pressure of
1 atm.)
80.7
24.8
-55.9
4
9.29
after samples
81.4
25.6
-55.8
-55.85
5
6
9.30
9.32
7
9.33
8
9.34
f samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added
w Std
ppm
C.
PPt
PH
factor
ppm
NH3 ppm
NSW Control
5.0
138.4
above
cut off
16.5
29
8.06
1.00
N.D.
N.D.
75-85% 4hr A PSDDA
5.0
136.7
above
cut off
16.0
30
8.05
1.00
N.D.
N.D.
75-85% 4hr UA PSDDA
5.0
137,9
above
cut off
16.5
28
8.07
1.00
N.D.
N.D.
75-85% 24hr UA PSDDA
5.0
133,4
20
126.5
50
16.8
28
7.93
1.00
0.60
0.01
75-85% El. G.B.
5.0
76.0
400
63.0
50
17.7
29
7.85
1.00
4.74
0.09
65-75% 4hr A PSDDA
5.0
135.3
above
cut off
16.0
30
8.24
1.00
N.D.
N.D.
65-75% 4hr UA PSDDA
5.0
141.4
above
cut off
16.2
30
8.06
1.00
N.D.
N.D.
65-75% 24hr UA PSDDA
5.0
144.6
above
cut off
17.0
29
7.89
1.00
N.D.
N.D.
65-75% El. G.B.
5.0
995
180
83.0
50
17.7
29
7.82
1.00
1.73
0.03
45-60% 4hr A PSDDA
5.0
*133,6
20
125.2
50
16.0
30
8.13
1.00
N.D.
N.D.
45-60% 4hr UA PSDDA
5.0
134,6
at
cut off
15.9
30
8.06
1.00
N.D.
N.D.
45-60% 24hr UA PSDDA
5.0
136-9
above
cut off
16.8
30
7.88
1.00
N.D.
N.D.
45-65% El. G.B.
5.0
74,§
400
61.6
50
17.4
29
7.78
1.00
4.78
0.08
<30% 4hr A PSDDA
5.0
131,8
above
cut off
16.0
30
8.23
1.00
N.D.
N.D.
<30% 4hr UA PSDDA
5.0
143.2
above
cut off
16.9
29
8.04
1.00
N.D.
N.D.
<30% 24hr UA PSDDA
5.0
142,8
above
cut off
17.0
29
7.92
1.00
N.D.
N.D.
<30% El. G.B.
5.0
15.4
150
83.4
50
17.4
29
7.87
1.00
2.18
0.04
NSW 24 hour
5.0
14S,Q
above
cut off
16.8
29
8.19
1.00
N.D.
N.D.
•Note: This reached 134.1 before 50ppm Std was put in.
N.D.= Non detection. Value was below method detection limit.
-------
Phase IIIA: Calculation of Total and Unionized Ammonia - Orion Probe Data
Exper Num
920704 DATE
13-Jul-92
DAY
Filename
0704DE48
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
82.1
26.0
-56.1
1 9.26
before samples
79.0
23.8
-55.2
-55.73
2 9.27
82.0
26.1
-55.9
-56.00
3 9.28
81.2
25.0
-56.2
4 9.29
after samples
85.4
28.6
-56.8
-56.27
5 9.30
83.4
27.6
-55.8
6 9.32
7 9.33
8 9.34
(assumes pressure of 1 atm.)
If samples were not diluted, enter 1 for dilution factor
Jar
Sample
ID
Sample Samp Diluent
ml mcl, if ml, if
read diluted added
mcl Cone Temp
Sample Std mv Std Degr Salin
mv added w Std ppm C. ppt
PH
Corrected Total
Dilution NH3
factor ppm
Free
(unionized)
NH3 ppm
NSW Control
5.0
151.5 above cut off
15.9 29 8.06
1.00
N.D.
N.D.
75-85% 4hr A PSDDA
5.0
134.9
50
118.4
50
15.7
30
8.07
1.00
0.50
0.01
75-85% 4hr UA PSDDA
5.0
131.3
50
116.3
50
16.4
30
7.93
1.00
0.57
0.01
75-85% 24hr UA PSDDA
5.0
136.4
50
119.3
50
16.4
30
7.85
1.00
0.48
0.01
75-85% El. G.B.
5.0
80.8
310
67.1
50
15.7
29
8.24
1.00
3.58
0.14
65-75% 4 hr A PSDDA
5.0
132**
50
125.4
50
15.6
28
8.06
1.00
N.D.
N.D.
65-75% 4hr UA PSDDA
5.0
138.7**
50
127.0
50
16.2
30
7.89
1.00
N.D.
N.D.
65-75% 24hr UA PSDDA
5.0
136**
50
127.5
50
16.4
28
7.82
1.00
N.D.
N.D.
65-75% El. G.B.
5.0
108.5
100
94.0
50
15.7
30
8.13
1.00
1.17
0.04
45-60% 4hr A PSDDA
5.0
135.3**
50
123.5
50
15.6
28
8.06
1.00
N.D.
N.D.
45-60% 4hr UA PSDDA
5.0
137**
50
122.2
50
16.3
30
7.88
1.00
N.D.
N.D.
45-60% 24hr UA PSDDA
5.0
138.4
50
121.0
50
16.2
30
7.78
1.00
0.47
0.01
45-60% El. G.B.
5.0
78.5
310
66.1
50
15.6
28
8.23
1.00
4.03
0.16
<30% 4hr A PSDDA
5.0
135.5**
50
127.7
50
15.7
28
8.04
1.00
N.D.
N.D.
<30 % 4hr UA PSDDA
5.0
136.5**
50
126.9
50
16.9
30
7.92
1.00
N.D.
N.D.
<30% 24hr UA PSDDA
5.0
137.4**
50
126.7
50
16.3
30
7.87
1.00
N.D.
N.D.
<30% El. G.B.
5.0
99.4
140
86.5
50
15.7
27
8.19
1.00
1.87
0.07
* NOTE: ISA put in before probe was ready; sample waited.
N.D.= Non detection. Value was below method detection limit.
-------
PHYSICAL MONITORING DATA
An Employee-Owned Company
-------
PHASE D1A ECHINODERM BIOASSAY PHYSICAL MONITORING DATA
Client EPA TESC 3-57
Experiment # 920704 & 920703 (CD)
Day 0 Day 1 Day 2
Date 8-Jul-92 9-Jul-92 10-Jul-92
Time
Observer M. Pelletier, C. Shcchan, C. Sheehan, M. Pelltier J. Dinsdale, M. Pelltier,
J. Dinsdale
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
< 30 4-A
16
30
7.2
8.23
15.7
28
7.7
15.7
28
7.8
8.04
45-60 4-A
16
30
7.1
8.13
16.7
29
5.6
15.6
28
7.9
8.06
65-70 4-A
16
30
6.2
8.24
15.8
29
7.6
15.6
28
8.0
8.06
75-80 4-A
16
30
6.4
8.05
15.8
29
7.6
15.7
30
7.7
8.07
SW 4-A
15
28
7.3
8.06
15.7
28
7.6
15.7
28
7.6
8.06
75-85 PSDDA
17.5
29
5.6
7.85
16.5
29
3.8
15.6
29
7.7
8.24
65-75 PSDDA
18
29
6.1
7.82
16.9
30
3.6
15.6
29
7.9
8.13
45-60 PSDDA
17
29
6.8
7.78
16.7
29
2.8
15.6
28
7.9
8.23
<30 PSDDA
17
29
3.4
7.87
16.5
29
0.4
15.7
27
7.9
8.19
SW PSDDA
17.2
28
6.9
N.D.
16.7
29
7.1
15.6
28
8
N.D.
<30 GB
17.8
28
2.5
7.87
16.5
30
3.9
15.6
28
7.9
8.19
45-60 GB
17.8
28
5.9
7.78
16.9
29
4.5
15.6
28
7.9
8.23
65-75 GB
17.8
29
5.6
7.82
16.5
29
0.5
15.7
30
7.8
8.13
75-85 GB
17.3
29
5.8
7.85
16.9
29
4.3
15.8
29
8
8.24
SW GB
17.3
29
7.2
N.D.
17.0
30
7.1
15.9
29
7.7
N.D.
SW 4-UA
16.4
29
7.2
8.06
16.6
29
6.8
16.3
29
6.8
8.06
<30 4-UA
16.9
29
5.7
8.04
16.4
30
3.9
16.9
30
4.8
7.92
45-60 4-UA
15.9
30
5.9
8.06
16.2
29
4.6
16.3
30
4.5
7.88
65-75 4-UA
16.2
30
6.2
8.06
16.4
30
5.2
16.2
30
5.2
7.89
75-85 4-UA
16.5
28
5.9
8.07
16.3
29
4.8
16.4
30
5.2
7.93
<30 24-UA
17
29
5.1
7.92
16.4
29
4.7
16.3
30
4.2
7.87
45-65 24-UA
16.8
30
5.1
7.88
16.7
29
4.4
16.2
30
4.1
7.78
65-75 24-UA
17
29
5.6
7.89
16.9
29
5.1
16.4
28
4.9
7.82
75-85 24-UA
16.8
28
4.7
16.7
29
4.6
16.4
30
4.0
7.85
SW 24-UA
16.8
29
7.0
8.19
16.3
28
7.0
16.4
30
7.0
8.05
Cd Hi 12.0 mg
16.1
30
7.3
N.D.
16.1
30
7.2
16.8
30
7.0
N.D.
Cd Lo 1.5 mg
16.8
30
7.5
N.D.
16.4
30
6.8
16.7
30
6.7
N.D.
-------
REFERENCE TOXICANT
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PHIIIAE
Cone.
Number
Exposed
Number
Resp.
Observed
Proportion
Responding
Adjusted
Proportion
Responding
Predicted
Proportion
Responding
Control
1.5000
3 . 0000
6.0000
100
100
100
100
8
14
29
92
0.0800
0.1400
0.2900
0.9200
0.0000
0.0350
0.2033
0.9102
0.1088
0.0014
0.2056
0.9095
Chi - Square Heterogeneity = 0.914
Mu = 0.591672
Sigma = 0.139405
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 0.755727 0.567653 ( -0.356873, 1.868327)
Slope 7.173351 0.887286 ( 5.434271, 8.912432)
Spontaneous 0.108838 0.022370 ( 0.064992, 0.152683)
Response Rate
-------
PHIIIAE
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
1.8509
1. 3892
2 .2198
EC 5.00
2.3034
1.8441
2 . 6615
EC10.00
2.5883
2.1416
2.9363
EC15.00
2.8002
2 .3666
3.1407
EC50.00
3.9055
3.5412
4 .2561
EC85.00
5.4469
4 .9642
6. 1561
EC90.00
5.8930
5.3293
6.7784
EC95.00
6.6218
5.9007
7.8435
EC99.00
8.2408
7.0999
10.3753
-------
PHIIIAE
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6 +
5+
4 +
-o
3 +
2+.
1+
o. .
0+
_+ + + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
PHASE IIIB
MM
An EmployoeOwned Company
-------
SEDIMENT CHEMISTRY VALUES
An Employee-Owned Company
-------
QA REVIEW
An abbreviated data quality evaluation (QA1) of data collected for this project was performed in accordance
with the PSDDA Guidance Manual, Data Quality Evaluation for Proposed Dredged Material Disposal
Projects, June 1989 (PTI, 1989). QA1 forms for each (batch B442 and B656) follow this brief review.
Overall, data quality and adherence to PSEP and PSDDA guidelines were acceptable. Analytical results were
reported int eh correct formats and appropriate QA/QC analyses (blanks, duplicates, triplicates, MS, MSD,
etc.) were performed for all samples except as noted below. Because these samples were highly
contaminated, the laboratory was unable to meet the required detection limits (i.e., PSDDA screening levels)
for some compounds. These compounds are noted in the QA1 forms.
All chemical analyses, except particle size distribution, were performed at Analytical Resources Incorporated
of Seattle, Washington. Particle size distribution was determined by Soil Technology, Inc. of Bainbridge
Island. Specific QA issues are addressed below for conventionals, metals, and organics.
Conventionals
Batch B442: All QA/QC analyses were completed by the laboratories with the exception for triplicate
samples for grain size.
Batch B656. All QA/QC analyses were completed by the laboratories with the exception of triplicate
samples for grains size. Holding time were exceeded for total sulfides, ammonia, total solids, total organic
carbon, and total volatile solids. Additionally, sediments for total sulfides were not properly preserved. As
mentioned in the text, the two samples in this batch were originally not scheduled to be chemically analyzed.
The decision to analyze these samples was made following the submittal of the first batch of samples (B442).
Concentrations of the parameters listed above for D1C4-25/75 and M1C2-25/75 are qualified with an E, as
an estimated value.
Metals
Batch B442: All QA/QC analyses were completed by the lab. The relative percent difference (RPD) between
analytical duplicates for mercury was outside of the 20% control limit at 28%. In addition, matrix spike
recoveries for arsenic, copper, zinc, and mercury fell outside of the control limit range (75-125%) due to the
high concentrations of these metals measured in the sediments. Low recoveries also suggest matrix
interferences. No qualifiers are recommended.
Batch 656: All QA/QC analyses were completed be the lab. No violations were reported.
Volatile Qraanics
Batch B442: All QA/QC analyses were completed by the lab. Ethylbenzene and 1,2,4-trichlorobenzene
exceeded matrix spike recovery control limits in the matrix spike and matrix spike duplicate. Because no
other QA/QC violations were reported for these compounds, data qualifiers are not recommended.
Batch B656: Samples were not analyzed for volatile organic compounds.
-------
Semivolatile Organic Compounds and Pesticides/PCBs
Batch B442: All QA/QC analyses were completed by the lab. The matrix spike recovery for acenaphthene
was measured outside of the 50-150% control limit range in D1C4-50/50. Moreover, no matrix spike
recovery was reported for pyrene due to the high concentration of this analyte reported in the sample and
the added interferences caused by a complex matrix. Surrogate recoveries were also affected by matrix
interferences. Samples with less than 60 percent recovery TCMX included the blank, the Sequim Bay
reference sediment, and D1C4-50/50 MS and MSD, and less than 60 percent decachlorobiphenyl (DCBP)
was recovered in the Sequim Bay reference sediment. As mentioned in the text, the laboratory felt that twice
the amount of surrogate/spike compound mixture was added at the time of extraction, causing the QA/QC
violation in the MS and MSD. Not surprising was the fact that detection limits for several compounds (PAHs,
phenols, benzoic acid, benzyl alcohol, pesticides, and PCBs) exceeded the PSDDA screening levels.
Detection limits were raised to account for matrix interferences. All samples (except M1) were reanalyzed
at diluted concentrations because analytes were detected above the linear calibration range.
System performance was monitored by running instrument blanks between samples. These data along with
instrument tuning and calibration results indicated that system performance was acceptable. No qualifiers
in addition to the laboratory's qualifiers are required.
Batch B656: All QA/QC analyses were completed by the lab. Samples were not frozen following collection
and were not extract within the 14 day holding time; consequently, all semivolatile organics, pesticides, and
PCBs results for M1C2-25/75 must be qualified as estimates (E). The surrogate, decachlorobiphenyl
(DCBP), was not recovered in M1C2-25/75 due to matrix interferences. The sample was reanalyzed at a
1 to 5 dilution, and the surrogate was recovered without a violation. Lower detection limits were also
achieved following dilution.
-------
CHECKLIST FOR CONVENTIONAL VARIABLES IN SEDIMENT
Project Name £PA lESC SAIC Project No: Ql -00^-01-/00^
Lab ^rc£-S |do Lab # Bt4Li,'2>-
Responsible Technician fate S-fe^rnoeile^
Reviewed by JoilK) Date checklist prepared _J
Date: Sampled sAffti - zhhT~ _____
Received by lab Zhohz-
Analysis began ArvmMi* thohi . Mteg/ii/^,Ts/frc, TVS E/hAz. . tohsia %lv>h
Problems noted (e.g., deviations from prescribed methods, analytical problems)
(jyao Sitf-
COMPLETENESS AND HOLDING CONDITIONS
Method (identify)
# Samples submitted
# Samples analyzed
Total
Total
Grain Size
TOC
TVS
Sulfides
Ammonia
Solids
Distribution
?$£?
?stV
?se?
"Plomb W
?se?
?s£?
C?
(o
G?
(s>
c?
4?
C>
(f
6?
L>
Holding conditions acceptable?3 (Y/N)
If no, identify samples
V
AVS
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures
Y
Sample detection limit provided, when applicable (total sulfides, ammonia)
Qualifiers defined (e.g., U = undetected)
W«> not- /jainiA
Y
- Checklists adopted from PSODA guidance manual: PTI, 1989.
-------
CHECKLIST FOR CONVENTIONAL VARIABLES IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
TOC Total #
Frequency 1 io
(minimum 1 per 20 samples)
Amount detected in blank "?S.O C
(not a PSEP control limit)
Certified Reference Materials
TOC Total f
Frequency
(minimum 1 per survey)
CRM used Nl6^> 2:K>4-
Within 95°o confidence interval9 J|J ^I'l .PC To R&-
°lZ. l(o
(not a PSEP control limit)
Analytical Replicates
Total it of samples
Fiequency (minimum
1 triplicate per
20 samples)c
TOC
TVS
G
Total
Sulfides
Ammonia
G?
Total
Solids
Grain Size
Distribution
AVS
J
l-triy I trip l -fry > fry I V-f yioKug.
J PSDDA recommended holding times for sediment conventionals
Variable
Grain Size
Total Solids, Total
Volatile Solids, and
Total Organic Carbon
Total Sulfides
Ammonia
Acid Volatile Sulfides2
Refrigerated at 4*C
6 months
14 days
7 days
7 days
no guidance
Frozen at -18*C
not recommended1
6 months
not applicable
not applicable
no guidance
Samples most not be frozen or dried before analysis
Allen, et al. (1991) recommend 14-day holding time at 4"C
b Recommended by PSEP (1986)
• Checklists adopted from PSDDA guidanc« manual: PTI. 1989. -
-------
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT
Project Name SAIC Project No: f)l C>*^>
Lab A^9l^t)Ca\ fec^Cl-b. IflQ. Lab # A -j^O^ F
Responsible Technician
Reviewed by Date checklist prepared io Mr*-
Date Sampled k/m/u - tlyI'yu _L_"
Received by lab
Analysis began
Problems noted (e g , deviations from prescribed methods, analytical problems)
N/o/ul—
All required documents submitted?" (Y/N) ^
Analytical method
COMPLETENESS AND HOLDING CONDITIONS
# Samples Submitted ^ # Samples Analyzed ^
Holding conditions acceptable? (Y/N) (14 days at 4°C) Y
no, identify samples
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures
Qualifiers defined (e.g., U = undetected)
\)tkruA bgJbvJ tidy
Sample detection limits (DL) provided for each analyte? (Y/N)
Y
X
Checklists adopted from PSOOA guidance manual: PTI, 1989. •
-------
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
Total H J
Frequency | P Q/f 12^ 5^'^
(minimum 1 per batch or 1 per 12-hour shift, whichever is more frequent)1'
Chemicals detected above 2.5 ;/y total in one or more blanks1'
jOwc
Analytical Replicates
Total // 1 (hS,KSD -
Frequency I
( ?0 samples - 1 per set of samples submitted to lab; :.:20 samples 1 triplicate
and additional duplicate for minimum of 5% total replication overall)1'
Samples/chemicals with -35% RPD or CVC
Matrix Spikes (not required if isotope dilution used)
Total # 1 HS ; imp <;^jpluSpiW MIC?. -SC/20 samples - 5% overall)1'
kJoV"
Chemicals within 70 - 150% recovery
l%l7o m HS j iVi
1)2-.^ -Tft'dtlofobwieAr ^
Detection Limits
Did any DL exceed SL? (Y/N)
If yes, detection limits exceeding SL (identify samples)
Volatiles
- Checklists adopted Irom PSDDA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Surrogate Recovery
Were surrogates added to all samples?0 (Y/N) Y
Identity compounds with <85 percent recovery11 (also identify samples)
ftjono
Method Blanks (Relative blank contamination) j
Was blank contamination >5 percent of any sample concentrations?1' (Y/N) _____
If yes, identify compounds and samples
Organic Compounds
The following documentation is needed for organic compounds:
A cover letter referencing or describing the procedure used and discussing any analytical
problems
Reconstructed ion chromatograms for GC/MS analyses for each sample
Mass spectra of detected target compounds (GC/MS) for each sample and associated
library spectra
GC/ECD and/or GC/flame ionization detection chromatograms for each sample
Raw data quantification reports for each sample
A calibration data summary reporting calibration range used [and
decafluorotriphenyfphosphine (DFTPP) and brornofluorobenzene (8FB) spectra and
quantification report for GC/MS analyses]
Final dilution volumes, sample size, wet-to-dry ratios, and instrument detection limit
Analyte concentrations with reporting units identified (to two significant figures unless
otherwise justified)
Quantification of all analytes in method blanks (ng/sample)
Method blanks associated with each sample
Recovery assessments and a replicate sample summary (laboratories should report all
surrogate spike recovery data for each sample; a statement of the range of recoveries
should be included in reports using these data)
Data qualification codes and their definitions.
PSEP control limit.
Control limits determined at the PSDDA Chemistry QA/QC and PSDDA Streamlining Workshop held
24 January 1991, U.S. Army Corps of Engineers, Seattle District.
- Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR METALS IN SEDIMENT
Project Name i£SC SAIC Project No. 01 ~ 03> -1 & k°\
Lab A'blvj'hQl ffg-SjWO Lab If
Responsible Technician
Reviewed by
JoVin Date ctiecklist prepared
Date: Sampled SH/U - „
ReceivecJ by lab ^ |
Analysis began ^
Problems noted (e.g., deviations from prescribed methods, analytical problems)
All required documents submitted?3 (Y/N) Y
Digestion procedure [Total Acid Digest (TAD) or Strong Acid Digest (SAD)] '
+- ckW ccxis-, j -f AT)
COMPLETENESS AND HOLDING CONDITIONS
# Samples submitted ^ # Samples analyzed ^
Holding conditions acceptable? (Y/N) [2 years frozen -18*C and 6 months 4*C for metals except mercury;
28 days frozen and 7 days 4*C (in glass) for mercury] Y
If no, identify samples
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures V
Qualifiers defined (e.g., U = undetected)
Xes, "e>p bnaKo»> oi- mpfewip rgpoH- coM^>"
also untkr f&bte> Whfcn t^k^i •
Sample detection limits (DL) provided for each analyte? (Y/N) Y }
X>Lj> e.XC£Adtd t>r al| sobmrftLd .
• Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR METALS IN SEDIMENT (cont.)
QA/QC SAMPLES
Preparation Blank
Total # 1
Frequency13 1 yt/f bdhyh
(minimum 5% or 1 per batch, whichever is more frequent)0
Chemicals observed above detection limits in one or more blanks0
f\l prv«—
Certified Reference Materials
Total # I
Frequencyb I D&V
)atch
(minimum 5% or 1 per batbh, whichever is more frequent)0
CRM used _
Chemicals outside 80-120% recovery0
(for chemicals without certified values, use matrix spike results)
Analytical Replicates
Total #
I
Frequency13 \ ptv \>
(minimum 5% or 1 per batch, whichever is more frequent)0
Samples/chemicals with >20% relative percent difference (RPD) or coefficient of
variation (CV)c
Matrix Spikes
Total # [
Frequency5 I |p^
(minimum 5% or 1 per batch, whichever is more frequent)0
Chemicals with recovery outside 75-125%c
Arsenic, Cofper- f tine.
% xiuwury rep^dtl wof y^lid., sample, -h>©
fWo- (jb*Vb| \wh
- Checklists adopted from PSOOA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR METALS IN SEDIMENT (cont.)
NOTE: The following information will be filled out by PSDDA personnel rather than the laboratory.
Detection Limits .
Did any DL exceed SL? (Y/N) N)
If yes, detection limits exceeding SL (identify samples)
Antimony Arsenic Cadmium
Copper Lead Mercury
Nickel Silver Zinc
Preparation Blanks (Relative blank contamination)
Are sample results -:5 times blank values in any samples7 (Y/N)
If yes identify elements and samples
10
Metals:
For metals, the data report package for analyses of each sample should include the following:
• Tabulated results in units as specified for each matrix in the analytical protocols, validated
and signed in original by the laboratory manager
• Any data qualifications and explanation for any variance from the analytical protocols
• Results for all of the QA/QC checks initiated by the laboratory
• Tabulation of instrument and method detection limits.
All contract laboratories are required to submit metals results that are supported by sufficient
backup data and quality assurance results to enable independent QA reviewers to conclusively
determine the quality of the data. The laboratories should be able to supply legible photocopies
of original data sheets with sufficient information to unequivocally identify:
• Calibration results
• Calibration and preparation blanks
• Samples and dilutions
• Duplicates and spikes
• Any anomalies in instrument performance or unusual instrumental adjustments.
For batches of 5 samples or less, the minimum QA checks should be a blank and the analysis of
a CRM (and matrix spikes for any analytes not certified in the CRM). In general, the priority of QA
checks for batches of *5 samples should be as follows: CRM > analytical replicate > matrix spikes.
PSEP control limit.
- Checklists adopted trom PSDDA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT
Project Name SAIC Project No.
Lab ffifcSmrdS, Ino Lab #
Responsible Technician t (tyc&i 11V
Reviewed by Ngk^S<»1^ Date checklist prepared
Date: Sampled k/hM-l- - thl^
Received by lab _§Mi^
Analysis began _ %r(r¥/4- d'Mt L&ic. J>/c-(b h'fo an^t*- le^S . Pe&V/fc&s - Sever?! WrHi faifcl ddoiv*
CC-elvA\»hs 3*d PB^O? 3ls° S^uteM.
All required documents submitted? (Y/N)
Analytical method %1sfo *(p<^ flfcHjfld- jVCJ&f,
COMPLETENESS AND HOLDING CONDITIONS
# Samples # Samples
Submitted Analyzed
A/B/N H 4
Pesticides/PCB M
Holding conditions acceptable? (Y/N) (1 year for frozen sediment or 14 d&ys at 4"C until extraction:
extracts must be processed within 40 days) ] -
If no, identify samples
Extract conditions accepta&lk? (Y/N) (1 year for frozen sediment or 14 days at 4"C) Y
If no, identify' samples
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures \
Qualifiers defined (e.g., U = undetected)
- Checklists adopted from PSDOA guidance manual: PTI, 1989. •
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
Total # 1 SWl/p|^k , 1 ffevf/yc*c
Frequency ^
(minimum 1 per extraction batch)0
Chemicals detected above 5 ^g total (for phthalates) and 25 nq totald
(for other organic compounds; lower levels may be appropriate for
pesticides and PCBs)
Certified Reference Materials
Total # I
Frequency |
(<50 samples - 1 per set of samples submitted to lab; >50 samples - 1 per 50
samples analyzed)0
CRM used SQ - \ BlWeWiL
Chemicals outside 95% confidence interval (for certified values)d,e
No ceHvfttdl Valuer 35% RPD or CV^
M&VtL
Matrix Spikes (not required for A/B/N if isotope dilution used) t , , v
Total # i kVhs\) Sevxi/o^( DQ4(DM -*>/SD)
Frequency® | p ao
(<20 samples - 1 per set of samples submitted to lab; 220 samples - 5% of total
samples)0
Chemicals outside 50-150% recovery6
AcenapHVm tts , \y>y % ftsfr
r»o4- cloe^-h? Co^cLM-brjhan .
- Checklists adopted from PSDDA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Detection Limits
Did any DL exceed SL? (Y/N)
If yes, detection limits exceeding SL (identify samples)
y UsV
A/B/N (PAH) A/B/N (phenols, benzoic A/B/N (other)
acid, benzyl alcohol)
PCB Pesticides
Surrogate Recovery (A/B/N)
Were surrogates added to all samples''' (Y/N)
Identify compounds with SO percent recovery"1''' (also identify samples)
N)0A4-
Surrogate Recovery (Pesticides/PCBs)
Were surrogates added to all samples?0 (Y/N) V
Identify samples with - BO percent surrogate recovery0
MdW* &UK, ScH, D\C4oc^o ns m MS3) - TcttX
SQ-I - PCB?
Method Blanks (Relative blank contamination)
For target compounds other than phthalates, was blank contamination > 5 percent of any
sample concentrations? (Y/N) NJ
If yes, identify compounds and samples
For phthalates, was blank contamination >50 percent of any
sample concentrations?0 (Y/N) N
If yes, identify compounds and samples
Checklists adopted from PSDDA guidance manual: PN, 1989. -
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Organic Compounds
The following documentation is needed for organic compounds:
• A cover letter referencing or describing the procedure used and discussing any analytical
problems
• Reconstructed ion chromatograms for GC/MS analyses for each sample
• Mass spectra of detected target compounds (GC/MS) for each sample and associated
library spectra
• GC/ECD and/or GC/flame ionization detection chromatograms for each sample
• Raw data quantification reports for each sample
• A calibration data summary reporting calibration range used [and
decafluorotriphenylphosphine (DFTPP) and bromofluorobenzene (BFB) spectra and
quantification report for GC/MS analyses]
• Final dilution volumes, sample size, wet-to-dry ratios, and instrument detection limit
• Analyte concentrations with reporting units identified (to two significant figures unless
otherwise justified)
• Quantification of all analytes in method blanks (ng/sample)
• Method blanks associated with each sample
• Recovery assessments and a replicate sample summary (laboratories should report all
surrogate spike recovery data for each sample; a statement of the range of recoveries
should be included in reports using these data)
• Data qualification codes and their definitions
Prototype sampling and analysis plan provided by David Fox, U.S. Army Corps of Engineers on 7
August 1991.
For batches of 5 samples or less, the minimum QA checks should be a blank and the analysis of
a CRM (and matrix spikes for any analytes not certified in the CRM). In general, the priority of QA
checks for batches of s5 samples should be as follows: CRM > analytical replicate > matrix spikes.
PSEP control limit.
Control limits based on PSDDA Chemistry QA/QC ana Streamlining Workshop held 24 January
1991, U.S. Army Corps of Engineers, Seattle District
- Checklists adopted from PSDDA guidance manual: PTt, 1989.
-------
jiyy^rfs - Piratic G»*po-"vk \q SdiWrh
Did £vy DL ZXCmA SU? Y
l£ Ai-echoiq -exceed/^ Sl.^icW'A Sd**pi£>^)
A/S/N (PM^ pl d'lU'o^ WC,4-5o/so cKU^, PIC4-50/SO MS.
D\CM -Sfc/s-o nsp
A/6/^, pi, j>i d'ito+/»o, ma-*** AMU*,
^.ter*M) ^bA'^
A/6/M /otw) Dl, 1>I , HI; Hia-5-^ dM'^ ^
Dic^-5^ <»*, W' h &
fC%3 "Dl, rilC2 -9>M DICM -S0/) DicM - 5&/5o ni, W ^
Ml
WVL&fc*, DIW-^OHSC., m
-------
CHECKLIST FOR CONVENTIONAL VARIABLES IN SEDIMENT
Project Name 1 ^ SAIC Project No: C)1 ~ C>3 ~
Lab RcSoOftES, ^ C- Lab # SM^- BCo5Co
Responsible Technician Kate* Stafefhoeller-
Reviewed by ,ToV)iO Date checklist prepared _!*hE
Date: Sampled %/nh-i. - xfrMz-
Received by lab _ q/^/i-L
Analysis began Tdt?|S^ TVS GrtikSl'W 1Z?/^
Problems noted (e.g., deviations from prescribed methods, analytical problems)
T°t»t v>yipfofcvVfcer
# Samples submitted
# Samples analyzed
2-
2-
>
'2-
2-
2_
-z.*
2-
¦z-
2—
AVS
Holding conditions acceptable? (Y/N)
nJ
If no, identify samples Tot^ I A/hr*
-------
CHECKLIST FOR CONVENTIONAL VARIABLES IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
TOC Total #
I
Frequency ^
(minimum 1 per 20 samples)
Amount detected in blank ^ PP"° ^ ^ c"
(not a PSEP control limit) J^+ec^
Certified Reference Materials
TOC Total # l
Frequency I
(minimum 1 per survey)
CRM used 2-3 O4^
Within 95% confidence interval?
(not a PSEP control limit)
Analytical Replicates
Total # of samples
Frequency (minimum
1 triplicate per
20 samples)0
TOC
2_
rFrv
TVS
i-fry
Total
Sulfides
tC'f
Ammonia
Sulfide, ur'pn.
Total
Solids
Grain Size
Distribution
-2-
AVS
fr-i Ifr'p
a PSDDA recommended holding times for sediment conventional
Variable Refrigerated at 4*C
Grain Size 6 months
14 days
Total Solids, Total
Volatile Solids, and
Total Organic Carbon
Total Sulfides
Ammonia
Acid Volatile Sulfides2
7 days
7 days
no guidance
Frozen at -18'C
not recommended1
6 months
not applicable
not applicable
no guidance
1 Samples most not be frozen or dried before analysis
2 Allen, et al. (1991) recommend 14-day holding time at 4'C
b Recommended by PSEP (1986)
- Checklists adopted from PSDOA guidance manual: PTI, 1989. ¦
-------
Mo Vota+taorgiesdock
C^r Mciw^.
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT
Date: Sampled
Received by lab
Analysis began
Problems noted (e.g., deviations from proscribed methods, analytical problems)
All required documents submitted9'1 (Y/N)
Analytical method
COMPLETENESS AND HOLDING CONDITIONS
Holding conditions acceptable? (Y/N) (14 days at 4°C)
If no, identify samples
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures
Qualifiers defined (e.g., U = undetected)
Sample detection limits (DL) provided for each analyte? (Y/N)
Project Name
Lab
Responsible Technician
Reviewed by
SAIC Project No:
Lab tt
Date checklist prepared
# Samples Submitted
# Samples Analyzed
- Checklists adopted from PSODA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
Total #
Frequency
(minimum 1 per batch or 1 per 12-hour shift, whichever is more frequent)b
Chemicals detected above 2.5 (jg total in one or more blanksb
Analytical Replicates
Total # __
Frequency
( - 20 samples - 1 per set of samples submitted to lab; >20 samples - 1 triplicate
and additional duplicate for minimum of 5% total replication overall)'1
Samples/chemicals with >35% RPD or CVC
Matrix Spikes (not required if isotope dilution used)
Total #
Frequency
(^20 samples - 1 per set of samples submitted to lab; >20 samples - 5% overall)6
Chemicals within 70 - 150% recovery0
Detection Limits
Did any DL exceed SL? (Y/N)
If yes, detection limits exceeding SL (identify samples)
Volatiles
- Checklists adopted from PSDDA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR VOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Surrogate Recovery
Were surrogates added to all samples?0 (Y/N)
Identify compounds with <85 percent recovery0 (also identify samples)
Method Blanks (Relative blank contamination)
Was blank contamination -5 percent of any sample concentrations9" (Y/N)
If yes, identify compounds and samples
Organic Compounds
The following documentation is needed for organic compounds:
A cover letter referencing or describing the procedure used and discussing any analytical
problems
•, Reconstructed ion chromatograms for GC/MS analyses for each sample
Mass spectra of detected target compounds (GC/MS) for each sample and associated
. library spectra
GC/ECD and/or GC/flame ionization detection chromatograms for each sample
Raw data quantification reports for each sample
A calibration data summary reporting calibration range used [and
decafluorotriphenylphosphine (DFTPP) and bromofluorobenzene (BFB) spectra and
quantification report for GC/MS analyses]
Final dilution volumes, sample size, wet-to-dry ratios, and instrument detection limit
Analyte concentrations with reporting units identified (to two significant figures unless
otherwise justified)
Quantification of all analytes in method blanks (ng/sample)
Method blanks associated with each sample
Recovery assessments and a replicate sample summary (laboratories should report all
surrogate spike recovery data for each sample; a statement of the range of recoveries
should be included in reports using these data)
Data qualification codes and their definitions.
PSEP control limit.
Control limits determined at the PSDDA Chemistry QA/QC and PSDDA Streamlining Workshop held
24 January 1991, U.S. Army Corps of Engineers, Seattle District.
- Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR METALS IN SEDIMENT
Project Name 'fESC SAIC Project No. 0^ -QCf\$~ Q3>"
Lab Inc • Lab #
Responsible Technician
Reviewed by Date checklist prepared I ^
Date: Sampled
Received by lab
Analysis began
Problems noted (e.g., deviations from prescribed methods, analytical problems)
All required documents submitted?3 (Y/N) \
Digestion procedure [Total Acid Digest (TAD) or Strong Acid Digest (SAD)]
S Ur •
COMPLETENESS AND HOLDING CONDITIONS
# Samples submitted ^ # Samples analyzed ^"*
Holding conditions acceptable? (Y/N) [2 years frozen -18*C and 6 months 4*C for metals except mercury;
28 days frozen and 7 days 4"C (in glass) for mercury] \
If no, identify samples
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures
Qualifiers defined (e.g., U = undetected)
KJowjl OvM.
Sample detection limits (DL) provided for each analyte? (Y/N)
^ (_ fvo
- Checklists adopted from PSDOA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR METALS IN SEDIMENT (cont.)
QA/QC SAMPLES
Preparation Blank
Total # ^
Frequency13 _J
(minimum 5% or 1 per batch, whichever is more frequent)0
Chemicals observed above detection limits in one or more blanksc
Nl(*VL~
Certified Reference Materials
Total it Nk)l^
Frequency1'
(minimum 5% or 1 per batch, whichever is more frequent)1"
CRM used
Chemicals outside 80-120% recovery0
(for chemicals without certified values, use matrix spike results)
Analytical Replicates
Total #
Frequency13
(minimum 5% or 1 per batch, whichever is more frequent)0
Samples/chemicals with > 20% relative percent difference (RPD) or coefficient of
variation (CV)c
Matrix Spikes
Total # [Op"-*-
Frequency"
(minimum 5% or 1 per batch, whichever is more frequent)c
Chemicals with recovery outside 75-125%°
- Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR METALS IN SEDIMENT (cont.)
NOTE: The following information will be filled out by PSDDA personnel rather than the laboratory.
Detection Limits
Did any DL exceed SL? (Y/N) ^
If yes, detection limits exceeding SL (identify samples)
Antimony Arsenic Cadmium
Copper Lead Mercury _
Nickel Silver Zinc
Preparation Blanks (Relative blank contamination)
Are sample results <:5 times blank values in any samples9 (Y/N)
A
If yes identify elements and samples ^ £ ^eadinfij "frw* SI3
Metals:
For metals, the data report package for analyses of each sample should include the following:
• Tabulated results in units as specified for each matrix in the analytical protocols, validated
and signed in original by the laboratory manager
• Any data qualifications and explanation for any variance from the analytical protocols
• Results for all of the QA/QC checks initiated by the laboratory
• Tabulation of instrument and method detection limits.
All contract laboratories are required to submit metals results that are supported by sufficient
backup data and quality assurance results to enable independent QA reviewers to conclusively
determine the quality of the data. The laboratories should be able to supply legible photocopies
of original data sheets with sufficient information to unequivocally identify:
• Calibration results
• Calibration and preparation blanks
• Samples and dilutions
• Duplicates and spikes
• Any anomalies in instrument performance or unusual instrumental adjustments.
For batches of 5 samples or less, the minimum QA checks should be a blank and the analysis of
a CRM (and matrix spikes for any analytes not certified in the CRM). In general, the priority of QA
checks for batches of s5 samples should be as follows: CRM > analytical replicate > matrix spikes.
PSEP control limit.
- Checklists adopted from PSDDA guidance manual: PTI, 1989. -
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT
Project Name £?P> -TESC 3,-Sq SAIC Project Mo 01-0W8-03 HPO^
ub . Inc,. Lab » _
1 r~
Responsible Technician Kak. 3tonw>elfg
Reviewed by Date checklist prepared
Date: Sampled %HHT- "
Received by lab _
Analysis began _ a/s/tz-
Problems noted (e.g., deviations from prescribed methods, analytical problems)
StVeral fevV/ftS anglers fgi'seJ dtfecWt Itwrh doe. jb wvA-fk -^u-tvuVix
cih-terWiqS . Dlft? guyTCxp+l W KllCZ. £S/?S~ a|3c ^ffge-te^ , Q|cdcI S d,
All required documents submitted?3 (Y/N) Y
Analytical method „• (YVdfitcd &Q&Q
COMPLETENESS AND HOLDING CONDITIONS
# Samples # Samples
Submitted Analyzed
A/B/N 2- 2-
Pestici&es/PCB
Holding conditions acceptable? (Y/N) (1 year for frozen sediment or 14 days at 4*C until extraction:
extracts must be processed within 40 days) f\lo
If no, identify samples HlCZ. 2.5/^S" PlCH
Extract conditions acceptable? (Y/N) (1 year for frozen sediment or 14 days at 4*C) K
If no, identify samples _ nti z^hs-, picm tt/^s-
FORMAT
Standard data report sheet
Concentrations in proper units and significant figures Y
Qualifiers defined (e.g., U = undetected)
ItcM "fekU .
• Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
QA/QC SAMPLES
Method Blank
Total # 1 SSMVolgWfcS, 1 fcsVPCSs
Frequency 1
(minimum 1 per extraction batch)0
Chemicals detected above 5 ^ total (for phthalates) and 25 iug totald
(for other organic compounds; lower levels may be appropriate for
pesticides and PCBs)
Certified Reference Materials
Total # NW^
Frequency .
(<50 samples - 1 per set of samples submitted to fab; >50 samples - 1 per 50
samples analy2ed)c
CRM used
Chemicals outside 95% confidence interval (for certified values)d,e
Analytical Replicates
Total #
Frequency
(<20 samples - 1 per set of samples submitted to lab; ^20 samples - 1 triplicate
and additional duplicate for minimum of 5% total replication)0
Samples/chemicals with >35% RPD or CV*
Matrix Spikes (not required for A/B/N if isotope dilution used)
Total #
Frequency®
(<20 samples - 1 per set of samples submitted to lab; ;>20 samples - 5% of total
samples)0
Chemicals outside 50-150% recovery®
- Checklists adopted from PSDOA guidance manual: PTI, 1989.
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Detection Limits , / * « ^ , •( ¦ • \
Did any DL exceed SL? (Y/N) Y (V Pl^ d.Mf*! On'3 )
If yes, detection limits exceeding SL (identify samples)
A/B/N (PAH) A/B/N (phenols, benzoic DlQ"l A/B/N (other)
acid, benzyl alcohol) W^\
PCB Pesticides
Surrogate Recovery (A/B/N)
Were surrogates added to all samples'^0 (Y/N)
Identify compounds with ¦ r>0 percent rocoveryd,e (also identify samples)
NbrtiL,
Surrogate Recovery (Pesticides/PCBs)
Were surrogates added to all samples?0 (Y/N) ^
Identify samples with - 60 percent surrogate recovery8
M1C2, 2T/3T- l)€c5 percent of any
sample concentrations7 (Y/N) Ni
If yes, identify compounds and samples
For phthalates, was blank contamination >50 percent of any
sample concentrations?0 (Y/N) |\)
If yes, identify compounds and samples
- Checklists adopted from PSDDA guidance manual: PTI, 1989.
-------
CHECKLIST FOR SEMIVOLATILE ORGANIC COMPOUNDS IN SEDIMENT (cont.)
Organic Compounds
The following documentation is needed for organic compounds:
• A cover letter referencing or describing the procedure used and discussing any analytical
problems
• Reconstructed ion chromatograms for GC/MS analyses for each sample
• Mass spectra of detected target compounds (GC/MS) for each sample and associated
library spectra
• GC/ECD and/or GC/flame ionization detection chromatograms for each sample
• Raw data quantification reports for each sample
• A calibration data summary reporting calibration range used |and
decafluorotriphenylphosphine (DFTPP) and bromofluorobenzene (BFB) spectra and
quantification report for GC/MS analyses]
• Final dilution volumes, sample size, wet-to-dry ratios, and instrument detection limit
• Analyte concentrations with reporting units identified (to two significant figures unless
otherwise justified)
• Quantification of all analytes in method blanks (ng/sample)
• Method blanks associated with each sample
• Recovery assessments and a replicate sample summary (laboratories should report all
surrogate spike recovery data for each sample; a statement of the range of recoveries
should be included in reports using these data)
• Data qualification codes and their definitions
Prototype sampling and analysis plan provided by David Fox, U.S. Army Corps of Engineers on 7
August 1991.
For batches of 5 samples or less, the minimum QA checks should be a blank and the analysis of
a CRM (and matrix spikes for any analytes not certified in the CRM). In general, the priority of QA
checks for batches of s5 samples should be as follows: CRM > analytical replicate > matrix spikes.
PSEP control limit.
Control limits based on PSDDA Chemistry QA/QC and Streamlining Workshop held 24 January
1991, U.S. Army Corps of Engineers, Seattle District
- Checklists adopted from PSDDA guidance manual: PTI, 1989. •
-------
EXPLANATION OF INORGANIC DATA REPORT CODES
The columns labeled 'PREP', 'C', and 'M' contain important information about your analyses. The codes are
defined below.
PREPARATION CODES
These 3-letter codes describe methods used to prepare samples for analysis:
AEN USEPA, Metals in air filters
AHM ARI, Mercury in air filters
AHN ARI, Metals in air filters
ANN NIOSH 7300, Metals in air filters
CAN AOAC (1984) 25.024, Metals in earthenware
DE6 HP A 600/4-79-020 218.5, Cr(Vl) in water
DMM DMN followed by TMM, Dissolved mercurv
DMN Filtered through .45u filter. Dissolved metals
EW6 F.WN followed by DE6
EWM EWN followed by TMM
EWN USEPA SW-846 1310, CP Toxicity
FHP ARI, Metals in tissue (HNO-yHClO})
FPP PSEP, Metals in tissue (HNOyHCIO,,)
FRM Journal, Mercury in tissue
FRN Journal, Metals in tissue (HNO3/H7O2)
KRN ARI, Concentration by coprecipitation
LEM USEPA, TCLP followed by TMM
LEN USEPA, TCLP Extraction >¦
MHM ARI, Mercury in miscellaneous materials
MHN ARI, Metals in miscellaneous materials
OAM ARI, Mercury in oil, grease or tar
OAN ARI, Metals in oil, grease or tar
PHM ARI, Mercury in wipes
PHN ARI, Metajs in wipes
RCC USEPA CLP, Water digestion, HCI matrix
RCN USEPA CLP, Water digestion, HNO3 matrix
REC EPA 600/4-79-020 4.1.4, HCI matrix
REI EPA 600/4-79-020 200.7 and 9.4
REN EPA 600/4-79-020 4.1.4, HNO3 matrix
RMA EPA 600/4-79-020 206.2
R WC USEPA SW-846 3005
SCC USEPA CLP, Soil digestion, HCI matrix
SCM USEPA CLP, Mercury in soil
SCN USEPA CLP, Soil digestion, HNO3 matrix
SEM EPA 600/4-79-020 245.5, Mercury in soi 1
SHE ARI, Metals in soil, 1 il; digestion
SRL Journal, Lithium meta-borate fusion
SPF PSEP, Metals in sediment, HF digestion
SSC Standard Methods 302C, Sb/Sn in soil
SSN Standard Methods :MJ2C, Soil digestion
SSS Standard Methods 302C, Ti in soil
SW6 USEPA SW-846 3060, Cr(VI) in soil
SWC USEPA SW-846 3050, HCI matrix
S WN USEPA SW-846 3050,IINO3 matrix
SVVR USEPA SW-846 Modified 3005, Sb by CJFAAb
TEC EPA 600/4-79-020 4.1.3, HCI matrix
TEG F.PA 600/4-79-020 272.1, Silver in water
TEI EPA 600/4-79-020 200.7 and 9.3
TEN EPA 600/4-79-020 4.1.3, HNO3 matrix
THG ARI, Silver in photographic solutions
TMM EPA 600/4-79-020 245.1, Mercury in water
TSC Standard Methods 302C, Sb/Sn in water
TSN Standard Methods 302D
TSS Standard Methods 302E, Ti in water
TWC USEPA SW-846 3010, HCI matrix
TWG USEPA SW-846 7760, Silver in water
TWN USEPA SW-846 3020, HNO3 matrix
WMN EPA 600/4-79-020, Preserved, undigested water
XSC Standard Methods 302B
CONCENTRATION CODES
These codes are used to qualify reported concentrations:
U No analyte was detected. The reported value is the lower limit of detection.
METHOD CODES
These codes signify the instrumental technique used for analysis:
CVA Cold Vapor Atomic Absorption Spectrophotometry
FLA Flame Atomic Absorption Spectrophotometry
GFA Graphite Furnace Atomic Absorption Spectrophotometry
ICP Inductively Coupled Plasma Atomic Emission Spectrometry
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
08/27/92
15:54:06
CIient:
Contact:
Project:
ID number:
Description:
Sampled:
Received:
Matrix:
SAIC
Tim Thompson
EPA Tesc. 3-57
Ml
/ /
08/10/92
Sediment
ARI job number: B4 4 2
ARI sample number: A
Released by:
ANALYTICAL
RESULTS
CAS Number
Analyte
Concentration
c
Prep
M
7440-36-0
Antimony
149 mg/kg-dry
SZF
GFA
7440-38-2
Arsenic
2 00 mg/kg-dry
SZF
GFA
7440-43-9
Cadmium
0.950 mg/kg-dry
SZF
GFA
7440-50-8
Copper
881 mg/kg-dry
SZF
ICP
7439-92-1
Lead
324 mg/kg-dry
SZF
ICP
7439-97-6
Mercury
0.994 mg/kg-dry
SCM
CVA
7440-02-0
Nickel
3 9 mg/kg-dry
SZF
ICP
7440-22-4
Silver
0.810 mg/kg-dry
SZF
GFA
7440-66-6
Zinc
909 mg/kg-dry
SZF
ICP
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
08/27/92
15:54:10
Client: SAIC
Contact: Tim Thompson
Project: EPA Tesc. 3-57
ID number: D1
Description:
Sampled: / /
Received: 08/10/92
Matrix: Sediment
ARI job number: B44 2
ARI sample number: B
Released by:
ANALYTICAL
R E
U L T S
CAS Number
Analyte
Concentration
C
Prep
M
7440-36-0
Antimony
7.3 mg/kq-dry
SZF
GFA
7440-38-2
Arsenic
2 0.7 mg/kg-dry
SZF
GFA
7440-43-9
Cadmium
1.4 3 mg/kg-dry
SZF
GFA
744 0-50-8
Copper
174 mg/kg-dry
SZF
ICP
7439-92-1
Lead
13 9 mg/kg-dry
SZF
ICP
7439-97-6
Mercury
0.429 mg/kg-dry
SCM
CVA
7440-02-0
Nickel
36 mg/kg-dry
SZF
ICP
7440-22-4
Silver
0.718 mg/kg-dry
SZF
GFA
7440-66-6
Z inc
290 mg/kg-dry
SZF
ICP
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
08/27/92
15:54:11
Client: SAIC
Contact: Tim Thompson
Project: EPA Tesc. 3-57
ID number: M1C2-50/50
Description:
Sampled:
Received:
/ /
08/10/92
Matrix: Sediment
ARI job number:
ARI sample number:
Released by:
B442
E
o
Si
ANALYTICAL
RESULTS
CAS Number
Analyte
Concentrat ion
C
Prep
M
7440-36-0
Antimony
60.1 mg/kg-dry
SZF
GFA
7440-38-2
Arsenic
77.4 mg/kg-dry
SZF
GFA
7440-43-9
Cadmium
0.403 mg/kg-dry
SZF
GFA
7440-50-8
Copper
355 mg/kg-dry
SZF
ICP
7439-92-1
Lead
141 mg/kg-dry
SZF
ICP
7439-97-6
Mercury
0.18 0 mg/kg-dry
SCM
CVA
7440-02-0
Nickel
29 mg/kg-dry
SZF
ICP
7440-22-4
Silver
0.415 mg/kg-dry
SZF
GFA
7440-66-6
Zinc
3 66 mg/kg-dry
SZF
ICP
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
08/27/92
15:54:12
Client:
Contact:
Proj ect:
ID number:
Description:
Sampled:
Received:
Matrix:
SAIC
Tim Thompson
EPA Tesc. 3-57
D1C4—50/50
/ /
08/10/92
Sediment
ARI job number:
ARI sample number:
B442
F
Released by:
ANALYTICAL
R E
[J L T S
CAS Number
Analyte
Concentration
c
Prep
M
7440-36-0
Antimony
3.4 mg/kg-dry
SZF
GFA
7440-38-2
Arsenic
9.78 mg/kg-dry
SZF
GFA
7440-43-9
Cadmium
0.716 mg/kg-dry
SZF
GFA
7440-50-8
Copper
80.5 mg/kg-dry
SZF
ICP
7439-92-1
Lead
66 mg/kg-dry
SZF
ICP
7439-97-6
Mercury
0.209 mg/kg-dry
SCM
CVA
7440-02-0
Nickel
3 5 mg/kg-dry
SZF
ICP
7440-22-4
Silver
0.344 mg/kg-dry
SZF
GFA
7440-6^-6
Z inc
126 mg/kg-dry
SZF
ICP
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
09/17/92
09:48:58
Client: SAIC
Contact: Tim Thompson
Project: TESC-3 57
ID number: D1C4 25/75
Description:
Sampled: / /
Received: 09/04/92
Matrix: Sediment
ARI job number: B656
ARI sample number: A
Released by:
ANALYTICAL RESULTS,
CAS Number
Analyte
Concentration
C
Prep
M
LOQ
7440-36-0
Antimony
1.15 mg/kg-dry
SZF
GFA
0. 671
7440-38-2
Arsenic
7.36 mg/kg-dry
SZF
GFA
1.341
7440-43-9
Cadmium
0.503 mg/kg-dry
SZF
GFA
0 . 671
7440-50-8
Copper
50.3 mg/kg-dry
SZF
ICP
1.341
7439-92-1
Lead
42.lmg/kg-dry
SZF
ICP
16. 77
7439-97-6
Mercury
0.078 mg/kg-dry
SCM
CVA
0.238
7440-02-0
Nickel
39.4 mg/kg-dry
SZF
ICP
6.707
7440-22-4
Silver
0.225 mg/kg-dry
SZF
GFA
0. 134
7440-66-6
Zinc
86.0 mg/kg-dry
SZF
ICP
2 . 683
-------
ANALYTICAL RESOURCES, INC.
Inorganic Laboratory Data Report
09/17/92
09:49:00
Client
Contact
Project
ID number
Description
Sampled
Received
Matrix
SAIC
Tim Thompson
TESC-3 57
M1C2 25/75
/ /
09/04/92
Sediment
ARI job number: B656
ARI sample number: B
Released by:
ANALYTICAL
RESULTS
CAS Number
Analyte
Concentration
C
Prep
M
LOQ
7440-36-0
Antimony
19.9 mg/kg-dry
SZF
GFA
3.353
7440-38-2
Arsenic
2 8.8 mg/kg-dry
SZF
GFA
3.353
7440-43-9
Cadmium
0.213 mg/kg-dry
SZF
GFA
0 .268
7440-50-8
Copper
171 mg/kg-dry
SZF
ICP
1.341
7439-92-1
Lead
69.3 mg/kg-dry
SZF
ICP
16.77
7439-97-6
Mercury
0.0925 mg/kg-dry
SCM
CVA
0. 183
7440-02-0
Nickel
2 2.8 mg/kg-dry
SZF
ICP
6.707
7440-22-4
Silver
0.247 mg/kg-dry
SZF
GFA
0. 134
7440-66-6
Zinc
178 mg/kg-dry
SZF
ICP
2 . 683
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semivolatiles by GC/MS
Lab ID: B442ADL
Matrix: Soils
Date Release Authorized:
Report prepared:
Sample No: Ml
QC Report No: B442-SAIC
Project: PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5t87
(206)621-6490
(206) 621-7523 (FAX)
09/09/92 MAC C pat
Date extracted: 08/12/92
Analyzed (FINN 4): 08/17/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 55.4 gm (Dry Weight)
Percent Moisture: 45.7%
pH: 7.6
Cone/Dilution: 1 to 4
5L
ML
ng/kg
Phenol
120
1200
50 J
Benzyl Alcohol
25
73
72 U
2-Methylphenol
20
72
52 J
4-Methylphenol
120
1200
72 U
Hexachtoroethane
1400
14000
72 U
2.4-Dim e th ylphenol
29
50
72 U
Benzoic Acid
400
690
720 U
Naphthalene
210
2100
140
Hexachlorobutadi&ne
29
290
72 U
2-Methylnaphthalene
67
670
100
Dimethyl Phthalate
160
-
72 U
Acenaphthylene
64
640
44 J
Acenaphthene
63
630
360
Dlbenzofuran
54
540
240
Dlethylph thalate
97
-
72 U
Fluorene
64
640
380
N-Nitrosodiphenylamine( 1)
28
220
72 U
Hexachlorobenzene
23
230
72 U
SL
ML
ug/Kg
Pentachlorophenol
100
690
280 J
Phenanthrene
320
3200
3400
Anthracene
130
1300
800
Di-n-Butylphthalate
1400
-
67 J
Fiuoranthene
630
6300
5900
Pyrene
430
7300
6200
Butytbenzylphthalate
470
-
110 M
Benzo(a)Anthracene
450
4500
2600
bis(2-Ethylhexyl)Phthalate
3100
-
860
Chrysene
670
6700
3800
Dl-n-Octyi Phthalate
6200
-
72 U
Benzo(b) Fiuoranthene
Benzo(k) Fiuoranthene
800
8000
4800
Benzo(a) Pyrene
680
6800
2500
IndenoO ,2,3-cd)Pyrene
69
5200
1900
Dibenz(a,h)Anthracene
120
1200
450
Benzo(ghl) Perylene
540
5400
1200
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries QA Limits Acid surrogate recoveries QA Limits
d5-Nitrobenzene 50.1% 23-120 d5-Phenol 53.8% 24-113
2-Fluoroblphenyl 62.7% 30-115 2-Fluorophenol 52.3% 25-121
dl4-p-Terphenyl 83.5% 18-137 2A6-Tribromophenol 57.6% 19-122
d4-1.2-Dichlorobenzene 57.2% 20-130 d4-2-Chlorophenol 57.8% 20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSODA Semivolatiles by GC/MS
Lab ID: B442E
Matrix: Soils
Date Release Authorized:
Report prepared: 09/09/92 MAC C pal
Sample No: M1C2-50/50
QC Report No:
Project:
B442-SAIC
PSDDA
EPATesc 3 57
VTSR: 08/10/92
Analytical
Chemists 8.
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621 -7!i23 (FAX)
Date extracted: 08/12/92
Analyzed (FINN 4): 08/17/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt:
Percent Moisture:
pH:
Cone/Dilution:
70.6 gm (Dry Weight)
30.6%
7.7
1 to 1
3L
ML
ng/Kg
Phenol
120
1200
130
Benzyl Alcohol
25
73
13 M
2-Methylphenol
20
72
42
4-Methylphenol
120
1200
30
Hexachloroe thane
1400
14000
14 U
2,4-Dimethylphenol
29
50
16M
Benzoic Acid
400
690
61 J
Naphthalene
210
2100
76
Hexachlorobutadiene
29
290
14 U
2- Methylnaphthalene
67
670
46
Dimethyl Phthalate
160
' -
14 U
Acenaphthylene
64
640
21
Acenaphthene
63
630
170
Dlbenzofuran
54
540
110
Diethylphthaiate
97
-
14 U
Fluoren©
64
640
170
N-Nitrosodiphenylamine(l)
28
220
14 U
Hexachlorobenzene
23
230
14 U
5L
ML
uy/Kg
Pentachlorophenol
100
690
230
Phenanthrene
320
3200
1300
Anthracene
130
1300
350
Di-n-Butylphthalate
1400
-
21
Fluoranthene
630
6300
1800 K
Pyrene
430
7300
2800 K
Butytbenzylphthalate
470
-
59
Benzo(a)Anthracene
450
4500
1200
bis(2-Ethylhexyl)Phthalate
3100
-
410
Chry serve
670
6700
1600 K
Di-rhOctyt Phthalate
6200
-
14 U
Benzo(b) Fluoranthene
Benzo(k)Fluoranthene
800
8000
2200
Benzo(a)Pyrene
680
6800
1100
lndeno(1,2,3-cd)Pyrene
69
5200
1000
Dibenz(a,h)Anthracene
120
1200
320
Benzo(ghi) Perylene
540
5400
620
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries
OA Limits
d5-Nitrobenzene
70.4%
23-120
2-Fluorobiphenyl
79.1%
30-115
dl4-p-Terphenyl
123%
18-137
d4-1,2-Dichlorobenzene
60.0%
20-130
Acid surrogate recoveries
QA Limits
d5-Phenol
77.6%
24-113
2-Fluorophenol
74.3%
25-121
2,4.6-Tribromophenol
65.1%
19-122
d4-2-Chlorophenol
75.6%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGAN ICS ANALYSIS DATA SHEET
PSDDA Semivolatiles by GC/MS
Lab ID: B442EDL
Matrix: Soils
Date Release Authorized
Report prepared
09/09192 MAC:C pat
Sample No: M1C2-50/50
Dilution
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
QC Report No:
Project;
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Date extracted: 08/12/92
Analyzed (FINN 4): 08/18/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt:
Percent Moisture:
pH: 7.7
Cone/Dilution: 1 to 4
70.6 gm (Dry Weight)
30.6%
SL
ML
HQ/Kg
Phenol
120
1200
92
Benzyl Alcohol
25
73
57 U
2-Methylphenol
20
72
29 J
4-Methylphenol
120
1200
57 U
Hexachioroe thane
1400
14000
57 U
2,4-Dimethylphenol
29
50
57 U
Benzoic Acid
400
690
57 U
Naphthalene
2 JO
2100
83
Hexachlorobutadiene
29
290
57 U
2-Methylnaphthalene
67
670
39 J
Dimethyl Phthaiate
160
-
57 U
Acenaphthytene
64
640
57 U
Acenaphthene
63
630
170
Dlbenzofuran
54
540
120
Dlethyiphthalate
97
-
57 U
Fluorene
64
640
180
N-Nltrosodiphenylamine( I)
28
220
57 U
Hexachlorobenzene
23
230
57 U
SL
ML
ug/Kg
Pentachlorophenol
100
690
200
Phenanthrene
320
3200
1500
Anthracene
130
1300
310
Di-n-Butylph thaiate
1400
-
57 U
Fluoranthene
630
6300
2500
Pyrene
430
7300
1800
Butylbenzylphthalate
470
-
57 U
Benzo(a)Anthracene
450
4500
1000
bis(2-Ethylhexyl)Phthalate
3100
-
340
Chrysene
670
6700
1500
Di-n-Octyi Phthaiate
6200
-
57 U
Benzo(b) Fluoranthene
Benzo(k)Fluoranthene
800
8000
2100
Benzo(a) Pyrene
680
6800
1000
Indenod ,2,3-cd) Pyrene
69
5200
740
Dibenz(a,h)Anthracene
120
1200
200
Benzo(ghi)Perylene
540
5400
540
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries QA Limits Acid surrogate recoveries OA Limits
d5-Nitrobenzene 52.0% 23-120 |d5-Phenol 51,4% 24-113
2-Fluorobiphenyt 71.6% 30-115 2-Fluorophenol 55.8% 25-121
dl4-p-Terphenyl 62.3% 18-137 2,4,6-T ribromophenol 77.4% 19-122
d4-12-Dichlorobenzene 60.6% 20-130 d4-2-Chlorophenol 61.9% 20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
U Indicales compound was analyzed
for but not detected at the given
detection limit.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semlvolatlles by GC/MS
Lab ID: B656-B
Matrix: Soils
Date Release Authorized:
Report prepared: 09/16/92
Sample No: M I C 2 25/75
QC Report No: B656-SAIC
Project: Elliott Bay
PSDDA-TESC-357
VTSR: 09/04/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-518
(206) 621-6490
(206) 621-7523 (FAX)
MAC: A rpr
Date extracted: 09/08/92
Analyzed (FINN 4): 09/10/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 75.8 gm (Dry Wt)
Percent Moisture: 24.2%
pH: 8.0
Cone/Dilution: 1 to 1
SL
ML
M9/Kg
Phenol
120
1200
160
Benzyl Alcohol
25
73
13 U
2-Methylphenol
20
72
13 J
4-Methylphenol
120
1200
23
Hexacnloroefhane
1400
14000
13 U
2.4-Dii i lethylpher iol
29
50
13 U
Benzoic Acid
400
690
130 U
Naphthalene
210
2100
23
Hexochlorobutadiene
29
290
13 U
2-Methylnaphthalene
67
670
24
Dimethyl Phthalate
160
-
13 U
Acenaphthylene
64
640
15
/Vcenaphthene
63
630
59
pibenzofuran
54
540
47
t)iethylphthalate
97
-
13 U
Fluorene
64
640
79
N-Nitrosodiphenylamine( 1)
28
220
13 U
Hexachlorobenzene
23
230
13 U
SL
ML
ng/Ka
Pentachlorophenol
100
690
98
Phenanthrene
320
3200
500
Anthracene
130
1300
110-
Di-n-Butylphthalate
1400
-
10M
Fluoranthene
630
6300
1100
F^rene
430
7300
1200
Butylbenzylphthalat©
470
-
54
Benzo(a)Anthracene
450
4500
470
bis(2-Ethylhexyl)Phthalate
3100
-
190~~
Chrysene
670
6700
570
Di-n-Octyl Phtbalate
6200
-
13~U
Benzo(b)Fluoranttiene
Benzo(k)Fluoranthene
800
8000
1000
Benzo(a)FV©ne
680
6800
47CT~
Indenod 2.3-cd)Pyrene
69
5200
280~~~
Dibenz(a,h)Anthracene
120
1200
75
Benzo(ghi)Perylene
540
5400
150
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries
QA Umits
d5-Nitrobenzene
67.3%
23-120
2-Fluorobiphenyl
58.3%
30-115
dl4-p-Terphenyl
105%
18-137
d4-12-Dlchlorobenzene
58.4%
20-130
Acid surrogate recoveries
d5-Phenol
68.5%
2-Fluorophenol
61.1%
2 A ,6-T ribromophenol
84.8%
d4-2-Chlorophenol
63.1%
QA Umit
24-113
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result Is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semivolatiles by GC/MS
Lab ID: B442B
Matrix: Soils
Date Release Authorized:
Report prepared:
Sample No: D1
QC Report No:
Project:
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
09/09/92 MAC:C pat
Date extracted: 08/12/92
Analyzed (FINN 4): 08/17/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 44.5 gm (Dry Weight)
Percent Moisture: 55.7%
pH: 7.3
Cone/Dilution: 1 to 1
SL
ML
M9/Kg
Phenol
120
1200
180
Benzyl Alcohol
25
73
23 U
2-Methylphenol
20
72
23 U
4-Methylphenol
120
1200
23 U
Hexachloroe thane
1400
14000
23 U
2.4-Dimethylphonol
29
50
23 U
Benzoic Acid
400
690
220 U
Naphthalene
210
2100
1700
Hexachlorobutadiene
29
290
23 U
2-Methylnaphthalene
67
670
530
Dimethyl Phthalate
160
-
23 U
Acenaphthylene
64
640
260
Acenaphthene
63
630
1900
Dibenzofuran
54
540
1200
Dlethylph thalate
97
-
23 U
Fluorene
64
640
1600
N-Nifrosodiphenylamine( 1)
28
220
23 U
Hexachlorobenzene
23
230
23 U
Base/neutral surrogate recoveries
OA Limits
d5-Nitrobenzene
69.6%
23-120
2-Fluorobiphenyt
83.0%
30-115
dl4-p-Terphenyl
150%
18-137
d4-l 2-Dlchlorobenzene
73.8%
20-130
SL
ML
ng/Kg
Pen tachlorophenol
100
690
110U
Phenanthrene
320
3200
3200 K
Anthracene
130
1300
2100 K
Di-n-Bulylph thalate
1400
-
23 U
Fluoranthene
630
6300
4300 K
Pyrene
430
7300
17000 K
Butylbenzylphthalate
470
-
23 U
Benzo(a)Anthracene
450
4500
6200 K
bis(2-Ethylhexy!)Phthaiate
3100
-
400
Chrysene
670
6700
8000 K
Dt-n-Octyt Phthalate
6200
-
23 U
Benzo(b) Fluoranthene
Bonzo(k)Fluoranthene
BOO
8000
17000 K
Benzo(a) Pyrene
680
6800
8500 K
lndeno(l ,2,3-cd)Pyrene
69
5200
5600 K
Dibonz(a,h)Anthracene
120
1200
2000
Benzo(ghi) Perylene
540
5400
3200 K
(1) Cannot be separated from diphenylamine
Acid surrogate recoveries OA Limits
dS-Phenol
81.4%
24-113
2-Fluorophenol
77.5%
25-121
2.4,6-T ribromophenol
71.8%
19-122
d4-2-Chlorophenol
82.2%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSODA SemivolatNes by GC/MS
Lab ID: B442BDL
Matrix: Soils
Date Release Authorized:
Report prepared: 09/09/9?
Sample No:
QC Report No:
Project:
D1
Dilution
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621 -7f>?3 (f AX)
MAC:C pat
Date extracted
Analyzed (FINN 4)
GPC Clean-up
Final Ext. Volume
08/12/92
08/18/92
Yes
1.0 ml
Sample Wt
Percent Moisture
pH
Cone/Dilution
44,5 gm (Diy Weight)
55.7%
7.3
1 to 20
•:,l
ML.
MO/Kg
Phenol
120
1200
450 U
Benzyl Alcohol
25
73
450 U
2-Methvlphenol
20
72
450 U
4-Methylphenol
120
12a)
450 U
t ioxachioroe thane
14iX)
14000
450 U
2.4-Dimethylphenol
2?
50
460 U
Benzoic Acid
4fX)
690
v; u
Naphthalene
.? 10
2100
2800
Hexachiorobutacliene
29
290
450 U
2-Methylnaphthalene
67
670
410 J
Dimethyl Phthalate
160
-
450 U
Acenaphthytene
64
640
450 U
Acenaphfhene
63
630
2000
Dibenzofuran
54
540
1400
Diethytphthatate
97
-
450 U
Fluorene
64
640
1700
N-Nitrosodiphenytamine( 1)
28
220
450 U
Hexachlorobenzene
23
230
450 U
ML
ug/Kg
Pen tachlorophe:)ol
100
690
22m u
Phenanthrene
320
3200
6500
Anthracene
130
1300 1
3300
Di-n Butytph thalat< :>
14CX)
460 U
Fluoranthene
630
6300
15000
Pyrene
430
7300
24000
Butylbenzylph thalato
470
4'A) U
Benzo(a) Anthracene
460
4M)
6100
t )is(2-Ethylh oxyDf 'h tt i ala ti ?
31 CM)
"i
i
Chrysene
670
6700
11000
Di-n-Octyt PhthalatiJ
6200
-
450 U
Benzo(b)Fluoranthene
Beazo(k) Fluoranthene
,'300
flOOO
20000
Benzo(a) Pyrene
680
6800
8400
IndenoO ,2,3-cd)Pyrene
69
5200
3700
Dibenz(a,h)Anthracene
120
1200
1500
Benzo(ghi)Perylene
540
.5400
2500
(1) Cannot be separated from ciiphenylamine
Base/neutral surrogate recoveries QA Limits Acid surrogate recoveries QA Limits
d5-Nitrobenzene 64.2% 23-120 d5-Phenol 68.7% 24-113
2-Fluorobiphenyl 83.0% 30-115 2-Fluorophenol 71.0% 25-121 '
d!4-p-Terphenyl 87.0% 18-137 2.4.6-Tribromophenol 71.1% 19-122
d4-12-Dichlorobenzene 77.8% 20-130 d4-2-Chlorophenol 71.8% 20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyle
found and confirmed by analyst but
with low specttal match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semivolatiles by GC/MS
Lab ID: B442F
Matrix: Soils
Date Release Authorized:,
Report prepared : 09/09/92 MAC.C, pat
Sample No: D1C4-50/50
QC Report No:
Project:
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621-7523 (FAX)
Date extracted: 08/12/92
Analyzed (FINN 4): 08/17/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 59.3 gm (Dry Weight)
Percent Moisture: 40.7%
pH: 7.7
Cone/Dilution: 1 to 1
SL
ML
ng/Kg
Phenol
120
1200
97
Benzyl Alcohol
25
73
17 U
2-Methylphenol
20
72
7.9 M
4-Methylphenol
120
1200
28
Hexachloroe thane
1400
14000
17 U
2,4-Dimethylphenol
29
50
18 M
Benzoic Acid
400
690
170 U
Naphthalene
210
2100
1700 K
Hexachlorobutadiene
29
290
17 U
2-Methylnaphthalene
67
670
490
Dimethyl Phthalate
160
-
17 U
Acenaphthylene
64
640
120
Acenaphthene
63
630
1800 K
Dlbenzofuran
54
540
1200
Diethyiphthalate
97
-
17U
Fluorene
64
640
1400
N-Nltrosodiphenytamlne( 1)
28
220
17 U
Hexachlorobenzene
23
230
17 U
SL
ML
ng/Kg
Pen tachlorophenol
100
690
84 U
Phenanthrene
320
3200
2400 K
Anthracene
130
1300
1300
Di-n-Butylph thalate
1400
-
17 U
Fluoranthene
630
6300
3400 K
Pyrene
430
7300
7500 K
Butylbenzylph thalate
470
-
17 U
Benzo(a)Anthracene
450
4500
2900 K
bis(2-Ethylhexyl)Phthalate
3100
-
160 M
Chrysene
670
6700
3700 K
Dl-n-Octyt Phthalate
6200
-
17 U
Benzo(b) Fluoranthene
Benzo(k) Fluoranthene
800
8000
7800 K
Benzo(a) Pyrene
680
6800
3500 K
IndonoU ,2,3-cd)Pyrene
69
5200
2300 K
Oi benz(a ,h) Anthracene
120
1200
820
Benzo(ghi) Perylene
540
5400
1300
(1) Cannot be separated from diphenytamine
Base/neutral surrogate recoveries
QA Limits
d5-Nitrobenzene
75.4%
23-120
2-Fluorobiphenyt
80.6%
30-115
dl4-p-Terphenyl
134%
18-137
d4-l 2-Dichlorobenzene
65.4%
20-130
Acid surrogate recoveries
QA Limits
d5-Phenol
78.2%
24-113
2-Fluorophenol
71.3%
25-121
2 A ,6-T ribromophenol
72.2%
19-122
d4-2- Chlorophenol
82.3%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSD DA Semivolatiles by GC/MS
Lab ID: B442FDL
Matrix: Soils
Date Release Authorized
Report prepared:
09/09/92 MAC C pat
Sample No: D1C4-50/50
Dilution
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
QC Report No:
Project:
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621 -7523 (FAX)
Date extracted: 08/12/92
Analyzed (FINN 4): 08/18/92
GPC Clean-uo: Yes
Final Ext. Volume: 1.0 mi
Sample Wt:
Percent Moisture:
pH:
Cone/Dilution:
59.3 gm (Dry Weight)
40.7%
7.7
1 to 10
5L
ML
i-ig/Kg
Phenol
120
1200
80 M
Benzyl Alcohol
25
73
170 U
2-Methyiphenol
20
72
170 U
4-Methylphenol
120
1200
170 U
Hexachloroethane
1400
14000
170 U
2,4-Dimethylphenol
29
50
170 U
Benzoic Acid
400
690
1700 U
Naphthalene
210
2100
3400
Hexachlorobutadiene
29
290
170 U
2-Methylnaphthatene
67
670
440
Dimethyl Phthalate
160
-
170 U
Acenaphthylene
64
640
170 U
Acenaphthene
63
630
1900
Dibenzofuran
54
540
1400
Dlethytphthalate
97
-
170 U
Fluorene
64
640
1600
N-Nitrosodiphenylamine( 1)
28
220
170 U
Hexdchlorobenzene
23
230
170 U
SL
ML
,ug/Kg
Pen tachlorophenol
100
690
840 U
Phenanthrene
320
3200
5000
Anthracene
130
1300
1800
Di-n-Butyiphthalate
1400
-
170 U
Fluoranthene
630
6300
9300
Pyrene
430
7300
9900
Butylbenzylphthalate
470
-
170 U
Benzo(a)Anthracene
450
4500
2700
bis(2-Ethylhexyl)Phthalate
3100
-
130 J
Chrysene
670
6700
4800
Di-n-Octyl Phthalate
6200
-
170 U
Benzo(b)Fluoranthene
Bo nzo(k) Fluoranthene
800
8000
7700
Benzo(a) Pyrene
630
6300
3300
lndeno( 1,2,3-cd)Pyrene
69
5200
1500
Dibenz(a,h)Anthracene
120
1200
590
Benzo(ghi) Perylene
540
5400
1000
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries
QA Limits
d5-Nitrobenzene
59.8%
23-120
2-Fluorobiphenyl
80.8%
30-115
dl4-p-Terphenyl
71.3%
18-137
d4-1,2-Dichlorobenzene
62.4%
20-130
Acid surrogate recoveries
QA Limits
d5-Phenol
61.6%
24-113
2-Fluorophenol
67.7%
25-121
2.4,6-Tribromophenoi
76.7%
19-122
d4-2-Ch!orophenol
66.0%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This (lag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for buf not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semlvolatlles by GC/MS
Lab ID: B656 - A
Matrix:
Soils
Date Release Authorized:
Report prepared: 09/16/92 MAC: A rpr
i:
Sample No: DIC4 25/75
QC Report No:
Project:
B656-SAIC
Elliott Bay
PSDDA-TESC-357
VTSR: 09/04/92
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621-7523 (FAX)
Date extracted: 09/08/92
Analyzed (FINN 4): 09/10/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 66.3 gm (Dry Wt)
Percent Moisture: 33.7%
pH: 7.6
Conc/Dilutlon: 1 to 1
SL
ML
ng/Kg
Phenol
120
1200
10 M
w
Benzyl Alcohol
25
73
15 U
y
2-Methylphenol
20
72
15 U
-
4-Methylphenol
120
1200
11 M
flexachloroe thane
1400
14000
15 U
2,4-Dimethylphenol
29
50
15 U
v
Benzoic Acid
400
690
150 U
-
Naphthalene
210
2100
840
Hexachlorobutadiene
29
290
15 U
-
2-Methylnaphthalene
67
670
290
J
'Dimethyl Phthalate
160
-
15 U
¦J
Acenaphthylene
64
640
88
Acenaphthene
63
630
830
-
Dibenzofuran
54
540
530
Diethylphthalate
97
-
15 U
y
fluorene
64
640
710
-
Kl-Nitrosodiphenylamine( 1)
28
220
15 U
Hexachlorobenzene
23
230
15 U
SL ML ng/Kg
Pentachlorophenol
100
690
75 U
Phenanthrene
320
3200
1500
Anthracene
130
1300
1000
DI-n-Butylphthalate
1400
-
15 U
Fluoranthene
630
6300
3000 K
Pyrene
430
7300
8500 K
Butyl benzylphthalate
470
-
15 U
Benzo(a)Anthracene
450
4500
2100 K
bis(2-Ethylhexyl)Phthalate
3100
-
75
Chrysene
670
6700
2500 K
Di-n-Octyl Phthalate
6200
-
15 U
Benzo(b)Fluoranthene
800
8000
4600 K
Benzo(k)Fiuoranthene
Benzo(a)Pyrene
680
6800
1900
lndeno( 1,2,3-cd)Pyrene
69
5200
880
Dlbenz(a ,h) Anthracene
120
1200
280
Benzo(ghi)Peryiene
540
5400
490
(1) Cannot be separated from diphenylamine
Base/neutral surrogate recoveries
OA Limits
d5-Nitrobenzene
64.5%
2-Fluoroblphenyl
57.2%
dl4-p-Terphenyl
113%
d4-l 2-Dlchlorobenzene
65.3%
23-120
30-115
18-137
20-130
Acid surrogate recoveries
QA Limits
d5-Phenol
72.1%
24-113
2-Fluorophenol
80.4%
25-121
2 A ,6-Trlbromophenol
76.6%
19-122
d4-2-Chtorophenoi
71.6%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyt® * detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
fa*s above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDOA Somivolatllos by GC/MS
Lab ID: B656 - A dl2
Matrix: Soils
±
Date Release Authorized:
Report prepared: 09/16/92 MAC: A rpr
Analytical
, ., „ , „ , Chemists &
Samplo No: D I C 4 25/75
Dilution
QC Report No: B656-SAIC 333 Ninth Ave North
Project: Elliott Bay Seattle, WA 98109-5187
PSDDA-TESC-357 (206)621-6490
VTSR: 09/04/92 (?06)6?1 7523 (FAX)
Dote extracted: 09/08/92
Analyzed (FINN 4): 09/11/92
GPC Cloan-up: Yes
t moi Ext. Volume: 1.0 ml
Sample Wt: 66,3. gin (Dry Wt)
Percent Moisture: 33.7%
pH: 7.6
Cone/Dilution: I to lo
ML
Phenol
Benzyl Aicor.oi
*-Methv!ph<»nai
¦let; iy.i
t
,:hiOIOi> -
"T~
?:)
' 2'J
4: KJ
- J —
1200
------
1 ?'X)
_M_g/K<-L
ISO IJ
! 50 U
""u>?Tr
-,U
'=Jl
•a
':.i0
: I j
7
r <
^
.
64
'340
e1. >0
" .,70
!! A}
;>3o
1 ';0 :
Pentachloropher 10!
Phecninih'orH;'
Anthrucer ie
Di-i i-kilvlp' ltt x jK ilt ¦
Hum. -j'A:
Dcf' 1
li ,, <
'lit
Di:jOn2v Ji : !thf 5'- -
Bt1: "i?o(< jr i:)p« .»rv!i:-•> •
(1) C< ii «-vr>* t if * sop< ut'j
SL
ML
M'J/-: !
100
690
320
3200
161)0" ~
i ;>o
1 'V";
1300
1 >00
1 ' •' A.'
"630 ""
"6300
6300
430
" 7300
1'
* ' /\J
4500
•
•>;o
Base/neutral surrogate recoveries
QA t.iitlits
d5-Nitrobenzene
66,5%
23-120
2-Fluorobiphenyl
65.2%
30-115
dl4-p-Terphenyl
87.5%
18-137
d4-12-Dichlorobenzene
64.2%
20-130
Acid surrogate recoveries
d5-Phenol
56.3%
24-T];n
2-Fluorophenol
68.3%
"?sT2r
2 A ,6-Tr ibror nophe nol
71.4%
l9^T22~
d4-2-Chlorophenol
61.0%
20-l3Q~
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA Semlvolatiles by GC/MS
Lab ID: B442SRMR
Matrix: Soils
Date Release Authorized:
Report prepared: 08/26/92 MAC ;C pat
Sample No: SQ-1
QC Report No:
Project:
B442-SAIC
PSDDA
EPA Tesc 3-57
VTSR: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621-7523 (FAX)
Date extracted: 08/12/92
Analyzed (FINN 4): 08/19/92
GPC Clean-up: Yes
Final Ext. Volume: 1.0 ml
Sample Wt: 34.3 gm (Dry Weight)
Percent Moisture: 32.6%
pH: 7.6
Cone/Dilution: 1 to 1
SL
ML py/Kg
GL
ML
Phenol
120
1200
210
Benzyl Alcohol
25
73
25 J
2-Methylphenol
20
72
29 U
4-Methylphenol
120
1200
170
Hexachloroe thane
1400
14000
29 U
2.4-Dimethylphenol
29
50
29 U
Benzoic Acid
400
690
120 J
Naphthalene
210
2100
68
Hexachlorobutadiene
29
290
29 U
2-Methylnaphthalene
67
670
66
Dimethyl Phthalate
160
-
29 U
Acenaphfhylene
64
640
44
Acenaphthene
63
630
79
Dibenzofuran
54
540
29 U
Diethylphthalate
97
-
29 U
Fluorene
64
640
76
N-Nitrosodiphenylamine( 1)
28
220
29 U
Hexachlorobenzene
23
230
29 U
Base/neutral surrogate recoveries
QA Limits
d5-Nitrobenzene
76.8%
23-120
2-Fluorobiphenyl
81.4%
30-115
dl4-p-Terphenyl
96.8%
18-137
d4-l 2-Dichlorobenzene
82.5%
20-130
M9/Kg
Pentachlorophenol
100
690
380
Phenanthrene
320
3200
1 10
Anthracene
130
1300
72
Di-n-Butylphfhnlate
1400
-
29 U
Fluoranthene
630
6300
75
Pyrene
430
7300
75
Butylbenzylphthalate
470
-
29 U
Benzo(a) Anthracene
450
4500
71
bis(2-Ethylhexyl)Phthalate
3100
-
58
Chrysene
670
6700
98
Di-n-Octyl Phthalate
6200
-
29 U
Benzo(b)Fluoranthene
800
8000
56
Benzo(k)Fluoranthene
800
8000
29 U
Benzo(a)Pyrene
680
6800
82
lndeno( 1,2,3 -cd)Pyrene
69
5200
36
Dibenz(a.h) Anthracene
120
1200
100
Benzo(ghi)Perylene
540
5400
82
(1) Cannot be separated from diphenylamine
Acid surrogate recoveries QA Limits
d5-Phenol
85.4%
24-113
2-Fluorophenol
80.8%
25-121
2 A6-Tribromophenol
101%
19-122
d4-2-Chlorophenol
87.5%
20-130
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is detected
in both the sample and the blank. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyt®
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA ANALYSIS BY METHOD 624/8240
Lab ID: B442 A
Matrix: Soils/Sediments
Sample: Ml
Data Release Authorized:
Report prepared 08/17/92 - MAC:P DWN
QC Report No
Project No
Date Received
B442-SAIC
EPA Tesc 3-57
08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621-7523 (FAX)
Instrument: FINN I
Date Analyzed: 08/13/92
Amount Analyzed: 2.97 g (dry wt.)
Percent Moisture: 52.2%
CAS Number
HQ/Kg
79-01-6
Trichloroethene
1.7 U
127-18-4
Tetrachloroethene
1.7 U
100-41-4
Ethylbenzene
1.7 U
1330-20-7
Total Xylenes
3.4 U
541-73-1
1,3-Dichlorobenzene
1.7 U
106-46-7
1,4-Dichlorobenzene
1.7 U
95-50-1
1,2-Dichlorobenzerie
1.7 U
120-82-1
1,2.4-Triichlorobenzene
1.7 U
Surrogate Recoveries
d8-Toluene
111%
Bromofluorobenzene
93.6%
d4-1,2-Dichloroethane
95.8%
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is found
in the blank as well as a sample. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA ANALYSIS BY METHOD 624/8240
Lab ID: B442 E
Matrix: Soils/Sediments
Data Release Authorized:
Report prepared 08/17/92 - MAC:P DWN
Sample: M1C2-50/50
QC Report No: B442-SAIC
Project No: EPA Tesc 3-57
Date Received: 08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Instrument: FINN I
Date Analyzed: 08/13/92
Amount Analyzed: 3.94 g (dry wt.)
Percent Moisture: 34.6%
CAS Number
HQ/Kg
79-01-6
Trichloroethene
1.3 U
127-18-4
Tetrachloroethene
1.3 U
100-41-4
Ethylbenzene
1.3 U
1330-20-7
Total Xylenes
2.5 U
541-73-1
1,3-Dichlorobenzene
1.3 U
106-46-7
1,4-Dichlorobenzene
1.3 U
95-50-1
1,2-Dichlorobenzene
1.3 U
120-82-1
1,2.4-Triichlorobenzene
1.3 U
Surrogate Recoveries
d8-Toluene
109%
Bromofluorobenzene
97.8%
d4-l ,2-Dichloroethane
93.2%
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is found
in the blank as well as a sample. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA ANALYSIS BY METHOD 624/8240
Lab ID: B442 B
Matrix: Soils/Sediments
Sample: D1
%\)
Data Release Authorized:
Report prepared 08/17/92 - MAC:P DWN
QC Report No:
Project No:
Date Received:
B442-SAIC
EPA Tesc 3-57
08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Instrument: FINN I
Date Analyzed: 08/13/92
Amount Analyzed: 2.11 g (dry wt.)
Percent Moisture: 65.1%
CAS Number
^g/Kg
79-01-6
Trichloroethene
2.4 U
127-18-4
Tetrachloroethene
2.4 U
100-41-4
Ethylbenzene
2.4 U
1330-20-7
Total Xylenes
4.7 U
541-73-1
1,3-Dichlorobenzene
2.4 U
106-46-7
1,4-Dichlorobenzene
2.4 U
95-50-1
1,2-Dichlorobenzene
2.4 U
120-82-1
1,2,4- Triichlorobenzene
2.4 U
Surrogate Recoveries
d8-Toluene
119%
Bromofluorobenzene
86.3%
d4-1,2-Dichloroethane
95.1%
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is found
in the blank as well as a sample. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ANALYTICAL
RESOURCES
INCORPORATED
ORGANICS ANALYSIS DATA SHEET
PSDDA ANALYSIS BY METHOD 624/8240
Lab ID: B442 F
Matrix: Soils/Sediments
Sample: D1C4-50/50
Wu
Data Release Authorized:
Report prepared 08/ 7 7/92 - MAC:P DWN
QC Report No
Project No
Date Received
B442-SAIC
EPA Tesc 3-57
08/10/92
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
Instrument: FINN I
Date Analyzed: 08/13/92
Amount Analyzed: 3.51 g (dry wt.)
Percent Moisture: 41.8%
CAS Number
HQ/Kg
79-01-6
Trichloroethene
1.4 U
127-18-4
Tetrachloroethene
1.4 U
100-41-4
Ethylbenzene
1.4 U
1330-20-7
Total Xylenes
2.1 M
541-73-1
1,3-Dichlorobenzene
7.4 U
106-46-7
1,4-Dichlorobenzene
1.4 U
95-50-1
1,2-Dichlorobenzene
1.4 U
120-82-1
1,2.4- Triichlorobenzene
1.4 U
Surrogate Recoveries
d8-Toluene
111%
Bromofluorobenzene
92.6%
d4-1,2-Dichloroethane
95.0%
Data Reporting Qualifiers
Value If the result is a value greater than or
equal to the detection limit, report
the value.
This flag is used when the analyte is found
in the blank as well as a sample. Indicates
possible/probable blank contamination.
Indicates compound was analyzed
for but not detected at the given
detection limit.
This flag is used when quantitated value
falls above the limit of the calibration
curve and dilution should be run.
Indicates an estimated value when
result is less than specified detection
limit.
M Indicates an estimated value of analyte
found and confirmed by analyst but
with low spectral match parameters.
-------
ORGAN ICS ANALYSIS DATA SHEET
PSDDA Pesticide/PCB by method 608/8080
B442-A
Soil/Sediments
Lab Sample ID:
Matrix:
Data Release Authorized: / /
¦u
Data Prepared: 09/03/92 - MAC: A rfa
Date Extracted
Date Analyzed
Sample Amount
Final Ext. Volume
08/12/92
08/19/92
56g Dry Wt
10 mis
Sample No.: M I
QC Report No.: B442-SAIC
Project: EPA TESC 3-57
PSDDA
VTSR: 08/10/92
GPC Cleanup: Yes
Alumina Cleanup: Yes
Sulfur Cleanup: No
Conc/Dil Factor: 1:1
ANALYTICAL
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave Norlh
Soatlle, WA 98109-5IE
(206) 621-6490
(206) 631 7r>?3 (F'AX)
CAS Number
SL
ML
ng/kg
58-89-9
Gamma-BHC (Lindane)
10
-
1.2 u
76-44-8
Heptachlor
10
-
1.2U
309-00-2
Aldrin
10
-
13 U
60-57-1
Dieldrin
10
5.0 U
72-55-9
4,4'-DDE
11 u
72-54-8
4,4'-DDD
29 U
50-29-3
4,4'-DDT
6.9
69
2.4 U
5103-74-2
Gamma-Chlordane
1.2 U
5103-71-9
Alpha-Chlordane
10
-
1.2 U
-
Aroclor-1242/1016
72 U
12672-29-6
Aroclor-1248
72 U
11097-69-1
Aroclor-1254
270
11096-82-5
Aroclor-1260
130
2500
280 U
Pesticide Surrogate Recovery % Rec. QC Limits
Decachlorobiphenyl (DCBP)
110%
60-150
Tetrachlorometaxylene (TCMX)
62.8%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value is less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for. but not detected at the given detection limit.
NA Indicates compound not analyzed.
FORM 1-PEST-S
-------
ORGANICS ANALYSIS DATA SHEET
PSDDA Pesticide/PCB by method 608/8080
Lab Sample ID: B442 - E
Matrix: Soil/Sediments
Data Release Authorized:
Data Prepared: 09/03/92 - 'MAC
Date Extracted: 08/12/92
Date Analyzed: 08/18/92
Sample Amount: 71gDry Wt
Final Ext. Volume: 10 mis
Sample No.: M IC 2 - 50/50
QC Report No.: B442-SAIC
Project: EPA TESC 3-57
PSDDA
VTSR: 08/10/92
GPC Cleanup: Yes
Alumina Cleanup: Yes
Sulfur Cleanup: No
Conc/Dil Factor: 1:1
analytical
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109-5187
(206) 621-6490
(206)621-7523 (fAX)
CAS Number
SL
ML
ug/kg
58-89-9
Gamma-BHC (Lindane)
10
-
1.2 u
76-44-8
Heptachlor
10
-
1.2U
309-00-2
Aldrin
10
-
2.5 U
60-57-1
Dieldrin
10
2.9 U
72-55-9
4,4'-DD£
6.3 U
72-54-8
4,4'-DDD
9.9
50-29-3
4.4-DDT
6.9
69
12 U
5103-74-2
Gamma-Chlordane
1.2 U
5103-71-9
Alpha-Chlordane
10
1.2 U
-
Aroclor-1242/1016
38 U
12672-29-6
Aroclor-1248
120 U
11097-69-1
Aroclor-1254
190
11096-82-5
Aroclor-1260
130
2500
150 U
Pesticide Surrogate Recovery % Rec. QC Limits
Decachlorobiphenyl (DCBP)
118%
60-150
Tetrachlorometaxylene (TCMX)
71.2%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value is less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for, but not detected at the given detection limit.
NA Indicates compound not analyzed.
FORM 1-PEST-S
-------
ORGAN ICS ANALYSIS DATA SHEET
PSDDA Pestidde/PCB by method 608/8080
Lab Sample ID: B656B
Matrix: Soil/Sedim^pts
Dato Release Authorized
Data Prepared: 09/17/92 - MAC: K kbs
Date Extracted: 09/08/92
Date Analyzed: 09/13/92
Sample Amount: 75.8g Dry Wt
Final Ext. Volume: 10 mis
Sample No.:
M1C2 25/75
QC Report No.:
B656-SAIC
Project:
EPA TESC-357
Elliott Bay
VTSR:
09/04/92
GPC Cleanup:
Ves
Alumina Cleanup:
Yes
Sulfur Cleanup:
No
Conc/Dil Factor:
1:1
ANALYTICAL
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206) 621-6490
(206) 621-7523 (FAX)
CAS Number
SL
ML
ng/kg
58-89-9
Gamma-BHC (Lindane)
10
-
0.8 U
76-44-8
Heptachlor
10
-
0.8 U
309-00-2
Aldrin
10
-
0.8 U
60-57-1
Dieldrin
10
1.6 U
72-55-9
4,4-DDE
2.1 U
72-54-8
4,4'-DDD
4.7 U
50-29-3
4,4'-DDT
6.9
69
5.0 U
5103-74-2
Gamma-Chlordane
0.8 U
5103-71-9
Alpha-Chlordane
10
-
0.8 U
-
Aroclor-1242/1016
40 U
12672-29-6
Aroclor-1248
50 U
11097-69-1
Aroclor-1254
71
11096-82-5
Aroclor-1260
130
2500
68 C
Pesticide Surrogate Recovery % Rec. QC Limits
Decachlorobiphenyl (DCBP)
NR
60-150
Tetrachlorometaxylene (TCMX)
76.9%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value is less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for. but not detected at the given detection limit.
C Indicates a probable value which cannot be confirmed due to interference.
NR Indicates the surrogate was not recovered due to interference.
FORM 1-PEST-S
-------
ORGAN ICS ANALYSIS DATA SHEET
PSD DA Pesticide/PCB by method 608/8080
Lab Sample ID: B656Bdl
Matrix: Soil/Sediments
Data Release Authorized:
Data Prepared: 09/17/92 -
Date Extracted: 09/08/92
Date Analyzed: 09/16/92
Sample Amount: 75.8g Dry Wt
Final Ext. Volume: 10 mis
Sample No.: M1C2 25/75
dilution
QC Report No.: B656-SAIC
Project: EPA TESC-357
Elliott Bay
VTSR: 09/04/92
GPC Cleanup: Yes
Alumina Cleanup: Yes
Sultur Cleanup: No
Conc/Dil Factor: 1:5
ANALYTICAL
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206)621-6490
(206) 621 -7523 (FAX)
CAS Number
SL
ML
ng/kg
58-89-9
Gamma-BHC (Lindane)
10
-
4.0 U
76-44-8
Heptachlor
10
-
4.0 U
309-00-2
Aldrin
10
-
4.0 U
60-57-1
Dieldrin
10
6.4 U
72-55-9
4,4-DDE
6.9
69
6.4 U
72-54-8
4,4-DDD
6.4 U
50-29-3
4.4-DDT
6.4 U
5103-74-2
Gamma-Chlordane
10
_
4.0 U
5103-71-9
Alpha-Chlordane
4.0 U
-
Aroclor-1242/1016
130
2500
64 U
12672-29-6
Aroclor-1248
64 U
11097-69-1
Aroclor-1254
60
11096-82-5
Aroclor-1260
74
Pesticide Surrogate Recovery % Rec. QC Limits
Decachlorobiphenyl (DCBR)
76.0%
60-150
Tetrachlorometaxytene (TCMX)
72.9%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value is less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for. but not detected at the given detection limit.
C Indicates a probable value which cannot be confirmed due to interference.
NR Indicates the surrogate was not recovered due to interference.
FORM 1 -PEST-S
-------
ANALYTICAL
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave North
Seattle, WA 98109 5187
(206) 621-6490
(206) 621-7523 (FAX)
CAS Number
SL
ML
ng/kg
58-89-9
Gamma-BFIC (Lindane)
10
1.8 u
76-44-8
Heptachlor
10
-
2.5 U
309-00-2
Aldrin
10
-
3.2 U
60-57-1
Dieldrin
10
6.4 U
72-55-9
4,4-DDE
6.2 U
72-54-8
4,4'-DDD
13 U
50-29-3
4,4'-DDT
6.9
69
2.4 U
5103-74-2
Gamma-Chlordane
8.5 U
5103-71-9
Alpha-Chlordane
10
-
1.2 U
-
Aroclor-1242/1016
96 U
12672-29-6
Aroclor-1248
200 U
11097-69-1
Aroclor-1254
180
11096-82-5
Aroclor-1260
130
2500
450 U
ORGANICS ANALYSIS DATA SHEET
PSDDA Pesticide/PCB by method 608/808
Lab Sample ID: B442 - B
Matrix: Soil/Sediments
A S ' /'
Data Release Authorized:
Data Prepared: 09/03/92 - MAC: A rpr
Date Extracted: 08/12/92
Date Analyzed: 08/19/92
Sample Amount: 45g Dry Wt
Final Ext. Volume: 10 mis
Sample No.: D I
QC Report No.: B442-SAIC
Project: EPA TESC 3-57
PSDDA
VTSR: 08/10/92
GPC Cleanup: Yes
Alumina Cleanup: Yes
Sulfur Cleanup: No
Conc/Dil Factor: 1:1
Pesticide Surrogate Recovery % Rec. QC Limits
Decachlorobiphenyl (DCBP)
136%
60-150
Tetrachlorometaxylene (TCMX)
68.0%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value is less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for. but not detected at the given detection limit.
NA Indicates compound not analyzed.
FORM l-PEST-S
-------
ORGAN ICS ANALYSIS DATA SHEET
PSDDA Pesticide/PCB by method 608/8080
Lab Sample ID: B442 - F
Matrix: Soil/Sediments
Data Release Authorized:
Data Prepared: 09/03/92 - MAC:
Date Extracted: 08/12/92
Date Analyzed: 08/19/92
Sample Amount: 59g Dry Wt
Final Ext. Volume: 10 mis
Sample No.: D I C 4 - 50/50
QC Report No.: B442-SAIC
Project: EPA TESC 3-57
PSDDA
VTSR: 08/10/92
GPC Cleanup: Yes
Alumina Cleanup: Yes
Sulfur Cleanup: No
Conc/Dil Factor: 1:1
ANALYTICAL
RESOURCES
INCORPORATED
Analytical
Chemists &
Consultants
333 Ninth Ave. North
Seattle, WA 98109-5187
(206)621-6490
(206) 621-7523 (FAX)
CAS Number
SL
ML
ng/kg
58-89-9
Gamma-BHC (Lindane)
10
-
1.2 U
76-44-8
Heptachlor
10
-
1.2 U
309-002
Aldrin
10
-
1.4 U
60-57-1
Dieldrin
10
2.4 U
72-55-9
4,4'-DDE
3.0 U
72-54-8
4,4'-DDD
11
50-29-3
4.4'-DDT
6.9
69
2.4 U
5103-74-2
Gamma-Chlordane
3.6 U
5103-71-9
Alpha-Chlordane
10
-
1.2 U
-
Aroclor-1242/1016
43 U
12672-29-6
Aroclor-1248
85 U
11097-69-1
Aroclor-1254
71
11096-82-5
Aroclor-1260
130
2500
150 U
Pesticide Surrogate Recovery % Rec. QC limits
Decachlorobiphenyl (OCBP)
97.3%
60-150
Tetrachlorometaxylene (TCMX)
62.8%
60-150
Data Qualifiers
Value If the result is a value greater than or equal to the detection limit, report the value.
J Indicates an estimated value when that value b less than the calculated detection limit.
X Indicates a value above the linear range of the detector. Dilution required.
S Indicates no value reported due to saturation of the detector.
D Indicates the surrogate was diluted out.
U Indicates compound was analyzed for. but not detected at the given detection limit.
NA Indicates compound not analyzed.
FORM 1 -PEST-S
-------
FULL MONITORING ELLIOT BAY
Sediment Grain Size Fractions
LAB
Sample
GRAIN SIZE
(Fractional
Percentages)1
ID
ID
GRAVEL
SAND
SILT
CLAY
>2mm
2.063mm
063.004mm
< 004mm
B442A
M-1
0
58
29
13
B442B
D-1
3
39
39
19
B442C
CARR2
0
86
10
4
B442D
CARR4
0
32
49
19
B442E
MIC2-50/50
1
75
15
9
B442F
DIC4-50/50
2
35
51
12
1 Fractional percentages interpolated from grain size graphs
SOIL TECHNOLOGY INC.
J-264
-------
Soil Technology, Inc.
Grain Size
Analysis
Project . EPA 357
Project No.: J-264
Location: EPA 357
Date : Men Sep 28 1992
U.S. STANDARD SIEVE SIZE
#4 #10 #20 #40 #60 #100 #200 #400
100
X.
uj
X
ill
UJ
UJ
D.
IMM
I I I I I—I 1
0.005
1000
100
111 I I I—I—I 1-
,0 ! U.J 01 0.05
GRAIN SIZE IN MILLIMETERS
o
UJ
z
<
h-
UJ
Ct
I—
2
UJ
O
tr
UJ
CL
100
0.01
0.001
GRAVEL
SAND
SILT OR CLAY
C G«R3E
fine
COARSE
MEDIUM
FINE
Symbol
Boiinq
!vj. Sample fio.
[>epth
Filename
Classification / Description
-*=•-
M - 1
B44_'A
B442A
SILT/ SAND
-h~
D- 1
B442B
B4423
SANDl SILT
-a-
C-f f-:l
84-IV
B4420
SILT
V SAND
LAFR4
B44.:fi
B442D
SAND) SILT
figure 1
-------
So« Technology. Inc.
100
90
30
X
i''
UJ
Oi
oc.
UJ
e>j
50
t; 40
o
a:
UJ
CL
30
20
10
Mill l—I r
) :
1 ; i :
, ! ;
i; i.
>-! -! •
!: i
1000
500
111 i
Grain Size
Analysis,
Project . EPA 55~
Project No.: J-264
Location: EPA 35?
Date : Mon Sep 2-S 1992
n-1—r
111! T—I—I 1 11 I l i 1—r -r r-
100
50
•y 111 fe
i. . STANDARD sieve size
*4 #ia #20 #40 #60 (100 #2L>0 #400
r-H-
Q
UJ
<
I—
UJ
cc
o
cr
LlJ
CL
-yrr-t-r-i—i—r~
10 5 1 U-J 0.1
GRAIN SIZE IN MILLIMETERS
100
0.01 0005
0.001
GRAVEL
SAND
cobbles
COARSE
FINE
COARSE
MEDIUM
FINE
SILT OR CLAY
Boring No. Sample No. Depth
MJC2-5Q/50 8442E •
Ijr50/50 B442ETR1
B442ETR2
J442ETR3
Filename Classification / Description
B442E SILTY SAND
B442ETR1 SILTY SAND
B442ETR2 SILTY SAND
B442E1R3 SILTi SAND
-------
SoN Technology. Inc.
Boring No. . DIC4-50/50
Sample No.. B442F
Tested by : TP
Filename : B442F
Project ; EPA 357
Project No.: J -264
Location: EPA 357
Date : Men Sep 23 1 y9^
U.S. STANDARD SIEVE SIZE
#4 #10 #20 #40 #60 #100 #200 #400
100
:<•>
I
. r»
U-!
UJ
a
40
50
:o
Ki
Mill | ! |
¦;CV
(\vl
••¦•x
i|.i. 1 ; §
\ :
Mi r ; "
tt-t-f
M-M
! i
u ¦ » i r i—i—i r
1000
500
100
ri i i i i—r-
50
i i i i i—r-
5
11 i I I i—i—r
0.5
10 J 1 0.1
GRAIN SIZE IN MILLIMETERS
I I i I i—r-
0.05
0.01 0005
10
20
30
Q
LJ
40 —
h-
LJ
ae
50
60
70
80
90
100
o
cc
UJ
CL
0.001
GPAVEL
T
SAND
CCbBLEi
COURSE
FINE
COARSE
MEDIUM
FINE
SILT OR CLAY
Classification
Visual Description
SANDY SILT
Remarks
Figure 3,
-------
"ECHNOLOGY
in ai son. rf stinc>
LETTER OF TRANSMITTAL
TO:
ATTENTION:
SUBJECT:
REFERENCE:
Analytical Resources, Inc.
333 Ninth Avenue North
Seattle, Washington 98109
Dave Mitchell
Elliott Bay Project ID No. TESC-357
Lab ID No. B656-A and B656-B
DATE: 09-14-92
JOB NO: J-270
We are sending the following items:
Date
Copies
Description
09-14-92
2
Sediment Grain Size Distribution (Figure 1)
09-14-92
1
Chain of Custody Records
These are transmitted for your use.
Remarks: Samples were tested in general accordance with Puget Sound Estuary
Protocol (Conventional Sediment Variables Particle Size March 1986). Values reported
are "apparent" particle size as organic material is included in the analysis. Please call if
you have any questions regarding this submittal or presentation of the data.
Best Regards,
SOIL TECHNOLOGY, INC.
Richard G. Sheets,
Vice President
-------
TESC-357
SEDIMENT GRAIN SIZE DISTRIBUTION
Sample ID: B656A
DIC4-25/75
Sieve Size -•>
Finef than Phi Size -->
Grain Size -->
63 % SOLIDS
No. 4: No 10: No 20: No 40: No 60 No. 140. No. 200: No 230:
4 5 6
>4750 4750-2000 2000-850 850-425 425-250 250-106 106-75 75-62 5 62 5-312 31 2-15 6 156-78
microns microns microns microns microns microns microns microns microns microns microns
7
7 8-3 9
microns
8
3 9-1.9
microns
9
19-0 9
microns
10
<0.9
microns
Balance
Percent Passing (%) -->
Fractional Percent (%) -->
Sample ID: B656B
MIC2-25/75
Sieve Size -->
Finer than Phi Size -->
Grain Size -->
100
0
No 4:
99
1
99
0
98
1
97
1
76 % SOLIDS
No. 10: No 20: No 40:
88
9
74
14
66
8
No 60: No. 140: No 200: No. 230:
36
30
19
17
>4750 4750-2000 2000-850 850-425 425-250 250-106 106-75 75-62 5
microns microns microns microns microns microns microns microns
4 5
62 5-31 2 31 2-15 6
microns microns
14
5
6
15 6-7 8
microns
11
3
7
7 8-39
microns
8
3 9-1.9
microns
9
1 9-0 9
microns
10
<09
microns
Balance
Percent Passing (%) -->
Fractional Percent (%) ->
99
1
99
0
98
1
94
4
83
11
37
46
23
14
19
4
13
6
10
3
SOIL TECHNOLOGY INC.
J-270
Page 1
-------
Chain of Custody Record &
Laboratory Analysis Request
ARI Client: ^ A XI C .
Client Contact: ^ r
Phone #:
-f*
Client Project ID: [ 6L S C, ~ 3 ^5 7
Samplers:
Sample ID
Date
Time
Matx
No
Cont
Lab ID
Dote: 9/V hd-
Page I of J
Number of coolers:
ANALYTICAL
RESOURCES
INCORPORATED
333 Ninth Ave. North
Seattle. WA 98)09-5187
(206) 621-4490.
(206) 621-7523 (FAX)
-J
r4 fC
C ^
i v.
<3
Analysis Required
Notes/Comments
ID/ cV
M tea ^
4
h(o5L
Received by:
(Signature)
Received by:
(Signature)
Printed Name:
OrninlA w. fraoM'Vi
Printed Name:
Printed Name:
Company:
Spi I "rVrhnaji-ic.^
Company:
Company:
Date- . lime:
?/**/?£ /
-------
LETTER OF TRANSMITTAL
TECHNOLOGY
.'f< iai i/in( , in physic.ai sou ifshnc,
.. i : •
TO:
ATTENTION:
SUBJECT:
REFERENCE:
Analytical Resources, Inc.
333 Ninth Avenue North
Seattle, Washington 98109
Dave Mitchell
Elliott Bay Project ID No. TESC-357
Lab ID No. B656-A and B656-B
DATE: 09-14-92
JOB NO: J-270
We are sending the following items:
Date
Copies
Description
09-14-92
2
Sediment Grain Size Distribution (Figure 1)
09-14-92
1
Cham of Custody Records
These are transmitted for your use.
Remarks: Samples were tested in general accordance with Puget Sound Estuary
Protocol (Conventional Sediment Variables Particle Size March 1986). Values reported
are "apparent" particle size as organic material is included in the analysis. Please call if
you have any questions regarding this submittal or presentation of the data.
Best Regards,
SOIL TECHNOLOGY, INC.
Richard G. Sheets,
Vice President
-------
TESC-357
SEDIMENT GRAIN SIZE DISTRIBUTION
Sample ID B656A
DIC4-25/75
Sieve Size -->
Finer than Phi Size -->
Grain Size -->
63
No. 4 No 10.
Io SOLIDS
No 20 No 40:
> 4750 4750-2000 2000-850 850-425
microns microns microns microns
No 60: No 140: No 200: No 230:
4 5 6 7 8 9 10
425-250 250-106 106-75 75-62 5 62 5-312 31 2-15 6 15 6-7 8 7 8-3 9 3.9-1.0 19-09 <0.9
microns microns microns microns microns microns microns microns microns microns microns
Balance
Percent Passing (%) -->
Fractional Percent (%) -•>
Sample 10: B656B
MIC2-25/75
Sieve Size -->
Finer than Phi Size -->
Grain Size ->
100
0
99
1
99
0
98
1
97
1
88
9
74
14
66
8
36
30
19
17
14
5
76 % SOLIDS
No 4: No 10: No 20: No 40: No 60: No 140 No. 200: No 230:
4 5 6
>4750 4750-2000 2000-850 850-425 425-250 250-106 106-75 75-62 5 62.5-31 2 31 2-15 6 15 6-7 8
microns microns microns microns microns microns microns microns microns microns microns
11
3
7
7 8-3.9
microns
8
3.9-1.9
microns
9
19-0 9
microns
10
<0.9
microns
Balance
Percent Passing (%) -->
Fractional Percent (%) ->
99
1
99
0
98
1
94
4
83
11
37
46
23
14
19
4
13
6
10
3
0
4
SOIL TECHNOLOGY INC.
J-270
-------
PHASE IIIB OYSTER
"Cm
An Employee-Owned Company
-------
LARVAL COUNTS
An Employee-Owned Company
-------
Oyster Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
ToIhI
Abnormal Survival
Initial Counts
TI
1
A
138
TI
1
B
153
TI
2
A
147
TI
2
B
120
TI
3
A
149
TI
3
B
119
TI
4
A
152
TI
4
B
149
TI
5
A
157
TI
5
B
138
Seawater
Control
4 Hour Unaerated
Ml
M1C2-50/50
I
A
122
6
128
1
B
122
7
129
2
A
128
4
132
2
B
135
5
140
3
A
113
5
118
3
B
134
5
139
4
A
121
4
125
4
B
128
4
132
5
A
146
2
148
5
B
158
5
163
1
A
71
12
83
1
B
56
4
60
2
A
77
2
79
2
B
98
1
99
3
A
79
8
87
3
B
95
10
105
4
A
68
2
70
4
B
88
6
94
5
A
64
5
69
5
B
69
8
77
0
1
A
93
9
102
1
B
98
4
102
2
A
97
5
102
2
B
76
4
80
3
A
93
4
97
3
B
89
5
94
4
A
86
2
88
4
B
73
2
75
5
A
67
0
67
no data
Page 1 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Initial seawater average count is 142
Control Survival = 95.2%
Normal = 96.5%
Abnormal = 3.5%
Final seawater control count is = 131
This number is used as 0% mortality for all
further calculations.
45.7 51.4 41.47 14.5 10.6 7.03
57.2 6.7
41.1 33.1 2.5 1.8
25.0 10
39.6 33.4 9.2 9.4
27.3 9.5
48.0 40.3 2.9 4.6
32.7 6.4
51.0 49.1 7.2 8.8
47.2 10.4
28.8 26.9 35.81 8.8 6.4 3.70
25.0 3.9
25.8 33.8 4.9 5.0
41.9 5.0
28.8 30.4 4.1 4.7
31.9 5.3
34.2 39.2 2.3 2.5
44.1 2.7
48.7 48.7 0.0 0.0
-------
Oyster Larval Data
Page 2 of 12
Station
Container
Number
Count
Rep
Total
Normal Abnormal Survival
% Mortality and
Replicate %
Mortality
Station
Replicate %
Abnormality
Station %
Abnormality
MIC2 - 25 75
D1
D1C4 - 50/50
D1C4 - 25V75
1.
A
109
5
1 14
16.6
20.0
18 29
4 4
2.2
1
B
100
0
100
23.5
0 0
2
A
124
4
128
5.1
7.0
3.1
2.0
2
B
119
1
120
9.0
0 8
3
A
97
0
97
25.8
311
0.0
0.0
3
B
83
0
83
36.5
0.0
4
A
103
0
103
21.2
17.4
0.0
0.0
4
B
113
0
113
13.5
0.0
5
A
107
4
111
18.1
15 8
3.6
3.5
5
B
113
4
117
13.5
3.4
0
1
A
88
5
93
32.7
28.5
29.30
5.4
2.7
1
B
99
0
99
24.3
0.0
->
A
86
2
88
34.2
36.9
2.3
1.8
2
B
79
1
80
39.6
1.3
3
A
91
10
101
30.4
30.8
9.9
6.6
3
B
90
3
93
31.1
3.2
4
A
99
12
111
24.3
28.1
10.8
8.1
4
B
89
5
94
31.9
5.3
5
A
92
4
96
29.6
22.3
4.2
6.2
5
B
111
10
121
15.1
8.3
1
A
118
8
126
9.7
8.2
12.88
6.3
6.3
1
B
122
8
130
6.7
6.2
2
A
97
6
103
25.8
15.1
5.8
5.9
2
B
125
8
133
4.4
6.0
3
A
133
4
137
0.0
8.7
2.9
5.3
3
B
108
9
117
17.4
7.7
4
A
103
6
109
21.2
18.9
5.5
3.7
4
B
109
T
111
16.6
1.8
5
A
115
5
120
12.0
13.5
4.2
3.4
5
B
111
3
114
15.1
2.6
0
1
A
110
6
116
15.8
13.5
13.41
5.2
3.0
1
B
116
1
117
11.2
0.9
2
A
123
6
129
5.9
12.0
4.7
4.6
2
B
107
5
112
18.1
4.5
3
A
121
123
7.4
18 1
1.6
2.4
3
B
93
3
96
28.8
3.1
4
A
103
4
107
212
216
3.7
2.4
4
B
102
1
103
22.0
1.0
5
A
140
6
146
0.0
1.8
4.1
3.2
5
B
126
3
129
3.6
2.3
1.54
5.06
4.91
3.10
-------
Oyster Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
4 HOUR AERATED
Seawater
Control
A
B
A
B
A
B
A
B
A
B
169
138
128
143
138
135
113
120
137
139
173
142
130
146
140
137
119
123
140
141
Ml
A
B
A
B
A
B
A
B
A
B
51
46
56
70
42
43
60
52
44
66
52
47
59
70
44
46
63
55
45
69
M1C2 - 50/50
A
B
A
B
A
B
A
B
A
B
66
72
78
90
95
62
78
86
61
64
66
77
79
90
95
62
78
86
62
69
M1C2 -25/75
A
B
A
B
A
B
A
B
A
B
99
82
90
90
79
79
81
73
114
85
99
83
91
90
80
80
81
73
115
85
Page 3 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 97.8%
Normal = 97.8%
Abnormal = 2 2%
Final seawater control count is = 136
This number is used as 0% mortality for all
further calculations.
62.5 64.3 61.03 1.9 2.0 3.70
66.2 2.1
58.8 53.7 5.1 2.5
48.5 0.0
69.1 68.8 4.5 5.5
68.4 6.5
55.9 58.8 4.8 5.1
61.8 5.5
67.6 59.6 2.2 3.3
51.5 4.3
51.5 49.3 44.71 0.0 3.2 1.66
47.1 6.5
42.6 38.2 1.3 0.6
33.8 0.0
30.1 42.3 0.0 0.0
54.4 0.0
42.6 39.7 0.0 0.0
36.8 0.0
55.1 54.0 1.6 4.4
52.9 7.2
27.2 33.5 35.88 0.0 0.6 0.57
39.7 1.2
33.8 33 8 1.1 0.5
33.8 0.0
41.9 41.9 1.3 1.3
41.9 1.3
40.4 43.4 0.0 0.0
46.3 0.0
16.2 26.8 0.9 0.4
37.5 0.0
-------
Oyster Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
D1 1 A 151 3 154
IB 102 1 103
2 A 78 0 78
2 B 81 0 81
3 A 93 3 96
3 B 109 4 113
4 A 117 2 119
4 B 96 0 96
5 A 103 3 106
5 B 110 1 111
D1C4 -50/50 1 A 104 0 104
IB 90 0 90
2 A 107 0 107
2 B 107 0 107
3 A 120 1 121
3 B 156 7 163
4 A 105 1 106
4 B 117 2 119
5 A 118 1 119
5 B 119 1 120
D1C4 25/75 1 A 107 0 107
IB 93 0 93
2 A 123 2 125
2 B 119 1 120
3 A 116 0 116
3 B 102 1 103
4 A 105 1 106
4 B 106 1 107
5 A 102 1 103
5 B 97 0 97
Page 4 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
0.0 12.5 24.63 1.9 1.5 1.50
25.0 1.0
42.6 41.5 0.0 0.0
40 4 0.0
31.6 25.7 3.1 3.3
19.9 3.5
14.0 21 7 1.7 0.8
29.4 0.0
24.3 217 2.8 1.9
19.1 0.9
23.5 28.7 17 43 0.0 0.0 0.94
33.8 0.0
21.3 21.3 0.0 0.0
21.3 0.0
11.8 5.9 0.8 2.6
0.0 4.3
22.8 18.4 0.9 1.3
14.0 1.7
13.2 12.9 0.8 0.8
12.5 0.8
21.3 26.5 21.32 0.0 0.0 0.63
31.6 0.0
9.6 11.0 1.6 1.2
12.5 0.8
14.7 19.9 0.0 0.5
25.0 1.0
22.8 22.4 0.9 0.9
22.1 0.9
25.0 26.8 1.0 0.5
28.7 0.0
-------
Oyster Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
24 HOUR UNAERATED
Seawater
Control
A
B
A
B
A
B
A
B
A
B
116
151
135
119
125
120
125
124
145
146
123
153
136
121
132
121
130
128
150
154
Ml
A
B
A
B
A
B
A
B
A
B
76
69
98
95
158
119
146
124
139
136
1
1
0
1
4
4
5
1
10
9
77
70
98
96
162
123
151
125
149
145
M1C2-50/50
1
1
2
2
3
3
4
4
5
5
A
B
A
B
A
B
A
B
A
B
72
67
101
110
118
93
95
102
108
113
3
3
7
2
0
5
1
0
7
2
75
70
108
112
118
98
96
102
115
115
M1C2-25/75
A
B
A
B
A
B
A
88
104
93
101
118
126
104
4
10
3
6
1
3
6
92
114
96
107
119
129
110
Page 5 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 94.8%
Normal = 96.9*
Abnormal = 3.1%
Final seawater control count is = 131
This number is used as 0% mortality for all
further calculations.
41.8 44.5 15.51 1.3 1.4 2.65
47.2 1.4
25.0 26.1 0.0 0.5
27.3 1.0
0.0 4.4 2.5 2.9
8.9 3.3
0.0 2.5 3.3 2.1
5.1 0.8
0.0 0.0 6.7 6.5
0.0 6.2
44.9 46.8 25.04 4.0 4.1 3.05
48.7 4.3
22.7 19.2 6.5 4.1
15.8 1.8
9.6 19.2 0.0 2.6
28.8 5.1
27.3 24.6 1.0 0.5
21.9 0.0
17.3 15.4 6.1 3.9
13.5 1.7
32.6 26.5 18.26 4.3 6.6 4.31
20.4 8.8
28.8 25.7 3.1 4.4
22.7 5.6
9.6 6.6 0.8 1.6
3.5 2.3
20.4 14.2 5.5 4.7
-------
Oyster Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
4 B 120 5 125
5 A no data
5 B
D1 1 A 136 5 141
IB 98 8 106
2 A 122 4 126
2 B 123 1 124
3 A 92 3 95
3 B 95 4 99
4 A 98 4 102
4 B 112 4 116
5 A 114 0 114
5 B 114 2 116
D1C4 -50/50 1 A 119 11 130
1 B 102 9 111
2 A 94 5 99
2 B 133 6 139
3 A 120 3 123
3 B 98 11 109
4 A 123 3 126
4 B 107 5 112
5 A 108 5 113
5 B 118 3 121
D1C4 25/75 1 A 106 5 111
IB 112 4 116
2 A 131 6 137
2 B 106 4 110
3 A 114 9 123
3 B 83 8 91
4 A 112 8 120
4 B 103 3 106
5 A 121 0 121
5 B 111 1 112
Page 6 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
8.1 40
0.0 12.5 15.88 3.5 5.5 3.14
25.0 7.5
6.6 6.2 3.2 2.0
5.8 0.8
29.6 28.4 3.2 3.6
27.3 4.0
25.0 19.6 3.9 3.7
14.2 3.4
12.7 12.7 0.0 0.9
12.7 1.7
8.9 15.4 14.27 8.5 8.3 5.22
21.9 8.1
28.0 14.0 5.1 4.7
0.0 4.3
8.1 16.5 2.4 6.3
25.0 10.1
5.8 11 9 2.4 3.4
18.1 4.5
17.3 13.5 4.4 3.5
9.6 2.5
18.8 16.5 15 88 4.5 4.0 4.25
14.2 3.4
0.0 9.4 4.4 4.0
18.8 3.6
12.7 24.6 7.3 8.1
36.4 8.8
14.2 17.7 6.7 4.7
21.1 2.8
7.4 11.2 0.0 0.4
15.0 0.9
-------
Oyster Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
GREENBOOK
Seawater
Control
A
B
A
B
A
B
A
B
A
B
117
112
115
117
119
120
141
160
140
140
120
114
121
117
120
121
143
161
142
141
Ml
A
B
A
B
A
B
A
B
A
B
47
49
57
53
56
55
53
44
54
46
9
0
8
0
8
3
0
2
3
2
56
49
65
53
64
58
53
46
57
48
MIC2 - 50/50
A
B
A
B
A
B
A
B
A
B
68
75
97
97
85
85
91
94
87
85
5
4
5
12
4
2
11
10
2
7
73
79
102
109
89
87
102
104
89
92
M1C2-25/75
A
B
A
B
A
B
A
121
110
115
97
76
82
92
4
2
0
2
0
1
0
125
112
115
99
76
83
92
Page 7 of 1
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 91.4%
Normal = 98.5%
Abnormal = 1.5%
Final seawater control count is = 128
This number is used as 0% mortality for all
further calculations.
63.3 62.5 59.88 16.1 8.0 5.98
61.7 0.0
55.5 57.1 12.3 6.2
58.6 0.0
56.3 56.7 12.5 8.8
57.1 5.2
58.6 62.1 0.0 2.2
65.7 4.3
57.8 61.0 5.3 4.7
64.1 4.2
46.9 44.2 32.55 6.8 6.0 6.49
41.5 5.1
24.3 24.3 4.9 8.0
24.3 11.0
33.6 33.6 4.5 3.4
33.6 2.3
29.0 27.8 10.8 10.2
26.6 9.6
32.1 32.9 2.2 4.9
33.6 7.6
5.5 9.8 18.% 3.2 2.5 1.53
14.1 1.8
10.2 17.3 0.0 1.0
24.3 2.0
40.7 38.3 0.0 0.6
36.0 1.2
28.2 28.2 0.0 0.0
-------
Oyster Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
4 B no data 0
5 A 125 3 128
5 B 140 7 147
D1 1 A 120 1 121
IB 117 1 118
2 A 98 4 102
2 B 111 1 112
3 A 127 3 130
3 B 102 3 105
4 A 123 0 123
4 B 108 0 108
5 A 109 3 112
5 B 114 3 117
D1C4 - 50/50 1 A 139 0 139
IB 137 2 139
2 A 127 7 134
2 B 121 2 123
3 A 169 2 171
3 B 145 1 146
4 A 122 2 124
4 B 104 0 104
5 A 119 2 121
5 B 85 2 87
D1C4 25/75 1 A 126 3 129
IB 119 2 121
2 A 120 2 122
2 B 122 3 125
3 A 125 4 129
3 B 108 4 112
4 A 133 5 138
4 B 138 3 141
5 A 131 2 133
5 B 118 2 120
Page 8 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
2.4 1.2 2.3 3.6
0.0 4.8
6.3 7.5 11.87 0.8 0.8 1.69
8.7 0.8
23.5 18.4 3.9 2.4
13.3 0.9
0.9 10 6 2 3 2.6
20.4 2.9
4.0 9.8 0.0 0.0
15.7 0.0
14.9 13.0 2.7 2.6
11.0 2,6
0.0 0.0 7.07 0.0 0.7 1.57
0.0 1.4
0.9 3.2 5.2 3.4
5.5 1.6
0.0 0.0 1.2 0.9
0.0 0.7
4.8 11.8 1.6 0.8
18.8 0.0
7.1 20.4 1.7 2.0
33.6 2.3
1.6 4.4 4.58 2.3 2.0 2.36
7.1 1.7
6.3 5.5 1.6 2.0
4.8 2.4
2.4 9.1 3.1 3.3
15.7 3.6
0.0 00 3 6 2.9
0.0 2.1
0.0 3.9 1.5 1.6
7.9 1.7
-------
Oyster Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
GREENBOOK WITH PSDDA COUNT
Seawater
Control
GreenbookVPSDDA
A
B
A
B
A
B
A
B
A
B
88
92
122
134
85
86
97
99
139
110
0
0
1
0
1
1
3
2
0
88
92
123
134
86
87
100
101
139
114
Ml
A
B
A
B
A
B
A
B
A
B
67
60
66
64
53
58
59
43
64
41
3
9
8
3
4
1
2
1
1
0
70
69
74
67
57
59
61
44
65
41
M1C2 - 50/50
A
B
A
B
A
B
A
B
A
B
37
38
39
27
65
65
51
37
57
56
40
39
42
28
68
71
54
41
59
63
M1C2 - 25/75
A
B
A
B
A
B
A
113
114
103
87
98
89
110
2
0
0
1
0
0
0
115
114
103
88
98
89
110
Page 9 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 74.8%
Normal = 98.9%
Abnormal = 1.1%
Final seawater control count is = 105
This number is used as 0% mortality for all
further calculations.
36.3 39.6 45.34 4.3 8.7 4.84
43.0 13.0
37.3 38.2 10.8 7.6
39.2 4.5
49.6 47.2 7.0 4.4
44.9 1.7
43.9 51.5 3.3 2.8
59.1 2.3
39.2 50.1 1.5 0.8
61.0 0.0
64.8 64.4 55.13 7.5 5.0 6.35
63.9 2.6
62.9 68.6 7.1 5.4
74.3 3.6
38.2 38.2 4.4 6.4
38.2 8.5
51.5 58.2 5.6 7.7
64.8 9.8
45.8 46.3 3.4 7.3
46.8 11.1
0.0 0.0 8.50 1.7 0.9 0.50
0.0 0.0
2.1 9.7 0.0 0.6
17.3 1.1
6.8 11.1 0.0 0.0
15.4 0.0
0.0 9.1 0.0 0.6
-------
Oyster Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
4 B 86 1 87
5 A 101 1 102
5 B 83 0 83
D1 1 A 83 0 83
IB 91 1 92
2 A 38 0 38
2 B 37 3 40
3 A 30 0 30
3 B 46 1 47
4 A 58 2 60
4 B 66 2 68
5 A 64 0 64
SB 81 0 81
D1C4 - 50/50 1 A 60 161
IB 65 0 65
2 A 69 0 69
2 B 58 0 58
3 A 83 3 86
3 B 81 1 82
4 A 90 1 91
4 B 74 1 75
5 A 55 0 55
5 B 52 0 52
D1C4 -25/75 1 A 88 0 88
IB 97 0 97
2 A 112 0 112
2 B 122 1 123
3 A 100 2 102
3 B 99 2 101
4 A 101 0 101
4 B 94 0 94
5 A 51 4 55
5 B 70 3 73
Page 10 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
18.3 11
4.0 12.5 1.0 0.5
21.1 0.0
21.1 17.3 43.54 0.0 0.5 1.70
13.5 1.1
63.9 64.4 0.0 3.8
64.8 7.5
71.5 63.9 0.0 1.1
56.3 2.1
44.9 41.1 3.3 3.1
37.3 2.9
39.2 31.1 0.0 0.0
23.0 0.0
43.0 40.6 34.70 1.6 0.8 0.88
38.2 0.0
34.4 39.6 0.0 0.0
44.9 0.0
21.1 22.1 3.5 2.4
23.0 1.2
14.4 22.1 1.1 1.2
29.7 1.3
47.7 49.1 0.0 0.0
50.6 0.0
16.3 12.1 13.46 0.0 0.0 1.61
7.8 0.0
0.0 0.0 0.0 0.4
0.0 0.8
4.9 5.4 2.0 2.0
5.9 2.0
4.0 7.3 0.0 0.0
10.6 0.0
51.5 42.5 7.3 5.7
33.5 4.1
-------
Oyster Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
CADMIUM REFERENCE TOXICANT
6 0mg/l
1
A
0
32
32
6.0mg/l
1
B
0
28
28
6.0mg/l
2
A
1
40
41
6.0mg/l
2
B
0
38
38
3.0mg/l
1
A
30
25
55
3 Omg/1
1
B
4
40
44
3 0mg/l
2
A
5
63
68
3 Omg/1
2
B
3
63
66
1 5mg/l
1
A
60
6
66
1 5mg/l
1
B
60
9
69
1,5mg/l
2
A
69
8
77
1.5mg/l
2
B
54
25
79
0.75 mg/L
1
A
118
3
121
0.75 mg/L
1
B
94
9
103
0.75 mg/L
2
A
106
7
113
0.75 mg/L
2
B
83
11
94
seawater
1
A
131
11
142
seawater
1
B
150
5
155
seawater
2
A
111
10
121
seawater
2
B
157
9
166
PHENOL REFERENCE TOXICANT
seawater
t
A
142
5
147
1
B
149
0
149
2
A
146
4
150
2
B
162
0
162
15.63
1
A
128
0
128
1
B
147
4
151
2
A
119
5
124
2
B
138
5
143
31.25
1
A
154
1
155
1
B
120
3
123
2
A
117
3
120
2
B
133
0
133
62.50
1
A
119
9
128
1
B
83
17
100
2
A
113
7
120
2
B
118
12
130
125
I
A
71
17
88
1
B
54
25
79
Page 11 of 12
% Mortality and
Abnormality
Replicate %
Mortality
Station %
Mortality
%
Abnormality
Replicate %
Abnormality
Station %
Abnormality
100.0
100.0
99.3
100.0
78.1
97.1
96.4
97.8
56.3
56.3
49.7
60.7
14.0
31.5
22.8
39.5
4.6
-9.3
19.1
-14.4
100.0
99.6
87.6
97.)
56.3
55.:
22.8
31.1
-2.4
2.4
99.82
92.35
55.74
26.96
0.00
100.0
100.0
97.6
100.0
45.5
90.9
92.6
95.5
9.1
13.0
10.4
31.6
2.5
8.7
6.2
11.7
7.7
3.2
8.3
5.4
100.0
98.8
68.2
94 1
11.1
21.0
5.6
8.9
5.5
6.8
99.39
81.12
16.04
7.28
6.16
3.40
0.00
2.67
0.00
12.48
-0.51
18.63
5.64
-5.30
17.95
20.00
9.06
18.63
43.25
22.74
19.32
51.45
63.08
1.7
1.3
6.0
12.1
6.3
14.5
30.9
21.0
57.3
1.52
9.06
10.43
25.98
56.07
3.4
0.0
2.7
0.0
0.0
2.6
4.0
3.5
0.6
2.4
2.5
0.0
7.0
17.0
5.8
9.2
19.3
31.6
1.7
1.3
1.3
3.8
1.5
1.3
12.0
7.5
25.5
1.52
2.54
1.40
9.77
20.17
-------
Oyster Larval Daia
Page 12 of 12
Container Count Totai % Mortality and Replicate % Station % % Replicate % Station %
Station Number Rep. Rep. Normal Abnormal Survival Abnormality Mortality Mortality Abnormality Abnormality Abnormality
2 A 71 12 83 51.45 54 9 14.5 14.9
2 B 61 11 72 58.29 15.3
-------
AMMONIA DATA
An Employee-Owned Company
-------
Phase 1MB Oyster: Calculation of Total and Unionized Ammonia - Samples collected at Test Initiation
Sample
Samp
Diluent
mcl
Cone
Temp
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
PPt pH
ppm
NH3 ppm
Exper Num
920814 DATE 26-Aug-92
DAY 0
Filename
2CR08141
analysis date
collected at initiation
oysters initiation
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
46.1
-10.7
-56.8
1
9.26
before samples
44.4
-12.0
-56.4
-56.60
2
9.27
-56.90
3
9.28
47.8
-9.4
-57.2
4
9.29
after samples
-57.20
5
9.30
6
9.32
Samples with mv > 139.7 are below the standard curve and serve as
7
8
9.33
9.34
estimates only. (< 0.5 ppm)
(assumes pressure of 1 atm.)
Sample
Samp
Diluent
mcl
Cone
Temp
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
NH3
(unionized)
Jar ID
read
diluted
added
mv
added w Std
ppm
C.
PPt pH
ppm
NH3 ppm
std 0.5
5.0
139.7
std 1.0
5.0
119.8
std 1.0
5.0
119.4
std 2.5
5.0
97.1
std 2.5
5.0
97.2
std 5.0
5.0
79.2
std 5.0
5.0
78.3
std 8.0
5.0
66.9
std 8.0
5.0
67.3
10.00
1.00
0.
¦ _
std ppm
0.10
std mv
0.50
1.00
1.00
2.50
2.50
5.00
5.00
8.00
8.00
Ml 4a init.*
5.0
152.7
19.5
29
7.87
0.29
0.01
Ml 4ua init.*
5.0
138.4
20.1
30
7.94
0.50
0.01
Ml 24 ua init.*
5.0
141.4
19.6
30
7.89
0.45
0.01
Ml GB psdda*
5.0
125.0
21.4
30
7.98
0.84
0.03
Dl 4a init*
5.0
151.2
19.8
29
7.84
0.31
0.01
Dl 4ua init*
5.0
149.8
19.7
30
7.90
0.32
0.01
Dl 24ua init*
5.0
150.5
19.8
30
7.83
0.31
0.01
Di GB init.*
5.0
117.6
21.4
28
7.93
1.12
0.03
MIC2 50 4a*
5.0
143.6
19.4
29
7.87
0.41
0.01
-------
Phase 1MB Oyster: Calculation of Total and Unionized Ammonia - Samples collected at Test Initiation
Sample
Samp
Diluent
mcl
Cone
Temp
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
PPt pH
ppm
NH3 ppm
MIC2 50 4ua*
5.0
144.6
19.4
30
7.94
0.39
0.01
MIC2 50 24ua*
5.0
144.0
20.0
30
7.83
0.40
0.01
MIC2 50 GB*
5.0
99.1
21.4
30
7.89
2.29
0.06
sea H20 24ua*
5.0
163.0
20.0
30
7.94
0.19
0.01
MIC2 25 4a*
5.0
151.6
19.7
29
7.84
0.30
0.01
MIC2 25 4ua
5.0
150.9
19.4
30
7.86
0.31
0.01
MIC2 25 24ua*
5.0
150.0
19.8
30
7.82
0.32
0.01
MIC2 25 GB psdda
5.0
115.0
21.6
28
7.89
1.24
0.04
DIC4 50 4a*
5.0
148.2
19.6
28
7.79
0.34
0.01
DIC4 50 4ua*
5.0
149.0
19.5
30
7.83
0.33
0.01
DIC4 50 24ua*
5.0
152.2
20.7
30
7.79
0.29
0.01
DIC4 50 GB*
5.0
108.8
20.6
30
7.81
1.58
0.03
DIC4 25 4a*
5.0
140.1
19.7
30
7.83
0.47
0.01
DIC4 25 4ua*
5.0
140.2
19.3
28
7.83
0.47
0.01
DIC4 25 24ua*
5.0
145.0
20.6
30
7.73
0.39
0.01
DIC4 25 GB*
5.0
101.9
20.3
28
7.76
2.06
0.04
std 1 * *
5.0
123.4
0.90
std 1**
5.0
123.3
0.90
std 2.5**
5.0
100.1
2.21
std 2.5**
5.0
99.1
2.29
std 5.0**
5.0
80.6
4.69
std 5.0**
5.0
80.7
4.67
std 8.0**
5.0
68.5
7.49
std 8.0**
5.0
68.8
7.41
std 10.0**
5.0
63.0
9.27
std 10.0**
63.1
9.24
* collected 25 Aug 92 (initiation), analyzed 26 Aug 92
cut off std, 0.5ppm NH3-N, mv= 139.7
pH meter slope = 90.4
Diluent = 28ppt
* 'second standard curve taken at end of day
-------
Phase IIIB Oyster: Calculation of Total and Unionized Ammonia - Orion Probe Data
Exper Num 920814 DATE 28-Aug-92 DAY 2 Filename 2CR08142
ANALYSIS collected at breakdown
Oysters breakdown
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
48.0
-8.4
-56.4
1
9.26
before samples
47.4
-8.7
-56.1
-56.25
2
9.27
-56.13
3
9.28
(assumes pressure of 1 atm.)
47.0
-9.0
-56.0
4
9.29
after samples
-56.00
5
9.30
6
9.32
7
9.33
Samples with mv > 137.8 are below the standard curve and should serve as
8
9.34
estimates only. (< 0.5 ppm)
If samples were not diluted, enter 1 for dilution factor
Sample Samp
Diluent
mcl
Cone
Temp
Corrected Total Free
Sample ml mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution NH3 (unionized)
Jar
ID read diluted
added
mv
added
w Std
ppm
C.
ppt
pH factor ppm NH3 ppm Mean
std 0.5
std 1.0
std 1.0
std 2.5
std 2.5
std 5.0
std 5.0
std 8.0
std 8.0
std 10.0
std 10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
137.8
122.1
10.00
121.8
std ppm
std mv
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.50
1.00
1.00
2.50
2.50
5.00
5.00
8.00
8.00
10.00
10.00
Ml 4a break*
5.0
158.8
18.8
30
8.10
1.00
0.22
0.01
Ml 4ua break*
5.0
154.8
18.9
30
7.98
1.00
0.26
0.01
Ml 24ua break*
5.0
151.7
18.9
30
7.89
1.00
0.29
0.01
Ml GB break*
5.0
129.8
19.1
30
8.80
1.00
0.72
0.12
Dl 4a break*
5.0
158.0
18.9
30
8.16
1.00
0.23
0.01
Dl 4ua break*
5.0
163.3
19.1
30
7.96
1.00
0.18
0.00
Dl 24ua break*
5.0
156.0
18.9
30
7.95
1.00
0.25
0.01
Dl GB break*
5.0
122.6
19.1
30
8.30
1.00
0.96
0.06
MIC2 50 4a*
5.0
164.1
18.9
30
8.17
1.00
0.18
0.01
MIC2 50 4ua*
5.0
152.5
19.1
30
7.97
1.00
0.28
0.01
MIC2 50 24ua*
5.0
151.6
19.1
30
7.92
1.00
0.29
0.01
-------
Phase IIIB Oyster: Calculation of Total and Unionized Ammonia - Orion Probe Data
MIC2 50 GB
5.0
117.0
19.1
30
8.23
1.00
1.21
-0.06
sea H20 24ua*
5.0
153.9
19.1
30
8.13
1.00
0.27
0.01
MIC2 25 4a*
5.0
160.0
18.9
30
8.19
1.00
0.21
0.01
MIC2 25 4ua*
5.0
148.8
18.9
30
7.92
1.00
0.33
0.01
MIC2 25 24ua*
5.0
157.5
19.3
30
7.92
1.00
0.23
0.01
MIC2 25 GB*
5.0
104.4
19.1
30
8.42
1.00
2.02
0.15
DIC4 50 4a*
5.0
159.6
18.9
29
8.22
1.00
0.21
0.01
DIC4 50 4ua*
5.0
155.9
19.1
30
7.93
1.00
0.25
0.01
DIC4 50 24ua*
5.0
160.8
19.3
30
7.91
1.00
0.20
0.00
DIC4 50 GB*
5.0
110.5
19.4
30
8.27
1.00
1.58
0.09
DIC4 25 4a*
5.0
157.5
19.0
30
8.28
1.00
0.23
0.01
DIC4 25 4ua*
5.0
143.3
18.9
30
7.92
1.00
0.41
0.01
DIC4 25 24ua*
5.0
154.3
19.1
30
7.87
1.00
0.26
0.01
DIC4 25 GB*
5.0
104.2
19.1
30
8.42
1.00
2.04
0.15
-------
PHYSICAL MONITORING DATA
An Employee-Owned Company
-------
PHASE mB ECfflNODERM BIOASSAY PHYSICAL MONITORING DATA
Client EPA TESC 3-57
Experiment # 920814 & 9208IS
Day 0 Day 1 Day 2
Date 25-Aug-92 26-Aug-92 27-Aug-92
Time 12:00 PM 12:00 AM 12:00 AM
Observer C. Sheehan ? C. Sheehan ??
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
24-UA SW
20.0
29
6.7
19.3
29
6.7
19.1
30
7.3
8.13
24-UA D1C4 - 25/75
20.6
28.5
4.7
19.4
29
4.8
19.1
30
4.6
7.87
24-UA D1C4 - 50/50
20.7
28
4.4
19.1
29
4.8
19.3
30
4.9
7.91
24-UA M1C2 - 25/75
19.8
30
4.0
19.4
28.5
4.7
19.3
30
4.9
7.92
24-UA M1C2 - 50/50
20.0
30
4.1
19.1
30
4.6
19.1
30
4.8
7.92
24-UA D1
19.8
30
4.5
19.1
30
4.7
18.9
30
4.9
7.95
24-UA Ml
19.6
30
3.9
19.0
30
4.4
18.9
30
4.8
7.89
4-UA SW
19.7
30
6.6
19.0
29
6.6
18.9
30
7.1
8.14
4-UA Ml
20.1
28
4.0
19.0
28
4.0
18.9
30
4.7
7.98
4-UA D1
19.7
30
4.9
18.9
28
4.6
19.1
30
4.9
7.96
4-UA M1C2 - 25/75
19.4
29
4.9
19.0
28.5
4.5
18.9
30
4.8
7.92
4-UA M1C2 - 50/50
19.4
28
4.7
19.1
28.5
4.4
19.1
30
4.9
7.97
4-UA D1C4 - 25/75
19.3
30
5.3
19.0
29
4.7
18.9
30
4.9
7.92
4-UA D1C4 - 50/50
19.5
30
5.3
18.9
28
4.7
19.1
30
5.0
7.93
4-A SW
19.3
30
7.0
18.7
28.5
7.4
18.8
30
8.0
8.18
4-A Ml
19.5
28
3.8
18.7
28
7.1
18.8
30
7.6
8.10
4-A D1
19.8
28
4.8
18.8
28
7.2
18.9
30
7.5
8.16
4-A M1C2 - 50/50
19.4
29
4.3
18.7
28
7.3
18.9
30
7.6
8.17
4-A M1C2 - 25/75
19.7
29
4.6
18.8
28
7.4
18.9
30
7.8
8.19
4-A D1C4 - 50/50
19.6
30
5.2
18.8
28
7.3
18.9
29
7.7
8.22
4-A D1C4 - 25/75
19.7
30
5.0
18.8
28.5
7.5
19.0
30
7.7
8.28
GB PSDDA SW
20.8
30
7.2
18.8
29.5
7.3
18.9
30
7.8
8.19
GB PSDDA D1
20.9
28
5.7
18.9
28
7.4
19.0
30
7.7
8.25
GB PSDDA Ml
21.4
28
5.4
18.9
28
7.2
19.0
30
7.8
8.26
GB PSDDA D1C4 - 2
21.0
29
5.9
19.0
28
7.7
19.0
30
7.8
8.32
GB PSDDA D1C4 - 5
21.6
30
5.3
19.1
29
7.7
19.1
30
7.6
8.29
GB PSDDA M1C2-
21.6
28
6.0
19.0
28.5
7.4
19.1
30
7.8
8.35
GB PSDDA M1C2 -
21.8
28
4.5
19.4
29
7.3
19.2
30
7.6
8.31
GB SW
21.3
30
7.3
19.7
29
7.3
19.3
30
7.7
8.30
GBD1
21.4
28
6.1
19.4
28
7.2
19.1
30
7.8
8.30
GB Ml
21.5
28
5.7
19.1
28
7.1
19.1
30
7.7
8.80
GB M1C2 - 50/50
21.4
28
5.5
18.9
30
7.0
19.1
30
7.4
8.23
GB M1C2 -25/75
20.3
28
6.7
18.8
28
7.3
19.1
30
7.8
8.35
GB D1C4 - 25/75
20.3
29
6.1
18.8
28
7.3
19.1
30
7.8
8.42
GB D1C4 - 50/50
20.6
30
5.3
18.8
29
7.1
19.4
30
7.1
8.27
CADMIUM
0.75
19.4
28
7.0
18.9
29
7.0
19.1
30
7.3
6
19.6
28
7.3
18.9
28
7.2
19.0
30
7.5
-------
REFERENCE TOXICANT
An Employee-Owned Company
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PIIIBO
Cone.
Control
0.7500
1.5000
3.0000
Number
Exposed
100
100
100
100
Number
Resp.
1
27
56
92
Observed
Proportion
Responding
0.0100
0.2700
0.5600
0.9200
Adjusted
Proportion
Responding
0.0000
0.2623
0.5554
0.9192
Predicted
Proportion
Responding
0.0104
0.2367
0.6082
0.8973
Chi - Square Heterogeneity = 2.013
Mu = 0.092708
Sigma = 0.303532
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 4.694569 0.099061 ( 4.500408, 4.888729)
Slope 3.294545 0.366194 ( 2.576805, 4.012286)
Spontaneous 0.010441 0.010156 ( -0.009464, 0.030346)
Response Rate
-------
PIIIBO
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
0.2436
0.1471
0.3388
EC 5.00
0. 3921
0.2688
0.5039
EC10.00
0.5055
0.3699
0.6240
EC15.00
0.6000
0.4583
0.7218
EC50.00
1.2380
1.0897
1.3892
EC85.00
2.5544
2.1960
3.1545
EC90.00
3 . 0319
2.5479
3.8963
EC95.00
3.9083
3.1632
5.3487
EC99.00
6.2924
4.7159
9.7515
-------
PIIIBO
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4 +
3 +
2 +
1+
0+
• • • •
o. .
_+ + + + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
OIIIBEC
Cone.
Control
1.5000
3 . 0000
6 . 0000
Number
Exposed
100
100
100
100
Number
Resp.
7
16
81
99
Observed
Proportion
Responding
0.0700
0.1600
0.8100
0.9900
Adj usted
Proportion
Responding
o.0000
0.1009
0.7966
0.9893
Predicted
Proportion
Responding
0.0657
0.1174
0.7717
0.9963
Chi - Square Heterogeneity = 1.72 3
Mu = 0.361157
Sigma = 0.155742
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 2.681058 0.331017 ( 2.032265, 3.329850)
Slope 6.420872 0.775047 ( 4.901780, 7.939964)
Spontaneous 0.065743 0.024574 ( 0.017578, 0.113909)
Response Rate
-------
OIIIBEC
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
0.9974
0. 7278
1.2192
EC 5.00
1.2734
0.9973
1.4931
EC10.00
1.4506
1.1780
1.6659
EC15.00
1.5840
1.3169
1.7952
EC50.00
2.2970
2.0701
2.5100
EC85.00
3.3309
3.0297
3.7690
EC90.00
3.6371
3.2804
4.1937
EC95.00
4.1432
3.6765
4.9312
EC99.00
5.2901
4.5224
6.7275
-------
OIIIBEC
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8 +
7 +
6+
5+
4 +
o
3 +
2 +
1+
0+
-+ + + + + H
EC01 EC10 EC25 EC50 EC75 EC90 E<
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
IIIBLCP
Cone.
Control
31.2500
62.5000
125.0000
Number
Exposed
100
100
100
100
Number
Resp.
2
10
26
56
Observed
Proportion
Responding
0.0200
0.1000
0.2600
0.5600
Adjusted
Proportion
Responding
0.0000
0.0812
0.2445
0.5508
Predicted
Proportion
Responding
0.0205
0.0761
0.2550
0.5455
Chi - Square Heterogeneity = 0.093
Mu = 2.052420
Sigma = 0.389327
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept -0.271715 0.767569 ( -1.776151, 1.232721)
Slope 2.568537 0.404891 ( 1.774951, 3.362123)
Spontaneous 0.020496 0.014076 ( -0.007093, 0.048086)
Response Rate
-------
IIIBLCP
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
i Limits
EC 1.00
14.0194
6.2606
21.7032
EC 5.00
25.8236
14.9721
35.0289
EC10.00
35.7650
23.6860
45.4942
EC15.00
44.5575
32.0974
54.5823
EC50.00
112.8287
95.0798
143.7456
EC85.00
285.7050
205.0729
519.9196
EC90.00
355.9432
243.6860
711.3580
EC95.00
492.9730
314.0566
1134.0961
EC99.00
908.0512
503.7079
2729.2690
-------
IIBLCP
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
vobit
10+
9+
8+
7 +
6+
5+
4 +
3 +
2 +
1+
0+
o. .
• • • •
• o • ~
-+ + + + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
PHASE IIIB ECHINODERM
-------
LARVAL COUNTS
An Employee-Owned Company
-------
Phase IIIB Echinoderm Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Tutu I
Abnormal Survival
Initial Counts
TI
1
A
162
TI
1
B
169
TI
2
A
131
TI
2
B
139
TI
3
A
140
TI
3
B
140
TI
4
A
149
TI
4
B
162
TI
5
A
166
TI
5
B
145
Seawater
Control
4-hour Unaerated
Ml
4-hour Unaerated
M1C2 - 50/50 4-hour Unaerated
1
A
112
6
118
1
B
114
6
120
2
A
124
19
143
2
B
142
14
156
3
A
132
11
143
3
B
125
11
136
4
A
136
6
142
4
B
163
8
171
5
A
139
7
146
5
B
146
7
153
1
A
57
9
66
1
B
75
20
95
2
A
72
16
88
2
B
64
17
81
3
A
59
32
91
3
B
62
35
97
4
A
85
33
118
4
B
70
19
89
5
A
86
11
97
5
B
78
12
90
0
1
A
115
9
124
1
B
135
10
145
2
A
132
12
144
2
B
74
15
89
3
A
74
10
84
3
B
80
8
88
4
A
120
8
128
4
B
106
13
119
5
A
60
15
75
5
B
56
15
71
Page 11 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Initial seawater average count is 150
Control Survival = 95.0%
Normal = 93.356
Abnormal = 6.7%
Final seawater control count is = 133
This number is used as 0% mortality for all
further calculations.
57.2 50.5 46.89 13.6 17.3 21.91
43.7 21.1
46.0 49.0 18.2 19.6
52.0 21.0
55.7 54.6 35.2 35.6
53.5 36.1
36.2 41.9 28.0 24.7
47.5 21.3
35.5 38.5 11.3 12.3
41.5 13.3
13.7 6.9 28.71 7.3 7.1 11.86
0.0 6.9
1.0 22.7 8.3 12.6
44.5 16.9
44 5 42.2 11.9 10.5
40.0 9.1
10.0 15.2 6.3 8.6
20.5 10.9
55.0 56.5 20.0 20.6
58.0 21.1
-------
Phase IIIB Echinoderm Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
M1C2 - 25-75 4-hour Unaerated
D1 4-hour Unaerated
D1C4 - 50/50 4-hour Unaerated
D1C4-25V75 4-hour Unaerated
•
1
A
92
18
110'
1
B
89
18
107
2
A
119
14
133
2
B
161
4
165
3
A
134
16
150
3
B
112
5
117
4
A
104
3
107
4
B
99
6
105
5
A
88
11
99
5
B
109
9
118
0
1
A
75
7
82
1
B
80
4
84
2
A
61
6
67
2
B
75
6
81
3
A
104
4
108
3
B
102
5
107
4
A
87
6
93
4
B
99
7
106
5
A
56
10
66
5
B
67
9
76
1
A
93
9
102
1
B
81
8
89
2
A
99
9
108
2
B
95
13
108
3
A
119
9
128
3
B
134
3
137
4
A
78
2
80
4
B
71
5
76
5
A
111
8
119
5
B
120
9
129
0
1
A
108
16
124
1
B
110
13
123
2
A
126
12
138
2
B
123
9
132
3
A
120
6
126
3
B
114
7
121
4
A
73
5
78
4
B
74
8
82
5
A
112
8
120
5
B
82
4
86
Page 12 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
31.0 32.1 19.08 16.4 16.6 8.83
33.2 16.8
10.7 5.4 10.5 6.5
0.0 2.4
0.0 8.0 10.7 7.5
16.0 4.3
22.0 23.9 2.8 4.3
25.7 5.7
34.0 26.1 11.1 9.4
18.2 7.6
43.7 41.9 39.53 8.5 6.6 7.81
40.0 4.8
54.2 49.0 9.0 8.2
43.7 7.4
22.0 22.7 3.7 4.2
23.5 4.7
34.7 30.2 6.5 6.5
25.7 6.6
58.0 53.9 15.2 13.5
49.7 11.8
30.2 34.7 24.96 8.8 8.9 7.02
39.2 9.0
25.7 27.2 8.3 10.2
28.7 12.0
10.7 5.4 7.0 4.6
0.0 2.2
41.5 44.1 2.5 4.5
46.7 6.6
16.7 13.4 6.7 6.8
10.0 7.0
19.0 18.2 21.83 12.9 11.7 7.70
17.5 10.6
5.5 6.6 8.7 7.8
7.7 6.8
10.0 12 2 4.8 5.3
14.5 5.8
45.2 44 9 6.4 8.1
44.5 9.8
160 27 2 6.7 5.7
38.5 4.7
-------
Phase IIIB Echinoderm Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
Seawater
Control
4-HOUR AERATED
4-hour aerated
Ml
2
2
3
3
4
4
5
5
1
1
2
2
3
3
4
4
5
5
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
125
103
117
123
103
122
105
104
105
110
3
0
0
0
0
0
0
0
2
6
10
10
14
13
12
14
20
25
25
22
77
84
66
55
94
89
44
78
82
97
135
113
131
136
115
136
125
129
130
132
80
84
66
55
94
89
44
78
84
103
M1C2-50/50
M1C2 - 25/75
1
1
2
2
3
3
4
4
5
5
1
1
2
2
3
3
4
4
5
5
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
A
B
28
16
0
30
14
4
17
9
32
28
50
39
83
58
79
73
40
45
38
55
53
51
80
56
86
73
52
67
60
88
99
75
25
15
17
23
61
79
69
58
81
67
80
86
100
77
69
76
92
116
149
114
108
73
96
96
101
124
107
113
Page 13 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 85.3%
Normal = 87.1 %
Abnormal = 12.9%
Final seawater control count is = 112
This number is used as 0% mortality for all
further calculations.
97.3 98.7 99.02 96.3 98.1 98.80
100.0 100.0
100 0 100 0 100.0 100.0
100.0 100.0
100.0 100.0 100.0 100.0
100.0 100.0
100.0 100.0 100.0 100.0
100.0 100.0
98.2 96.4 97.6 95.9
94.6 94.2
74.9 80.3 84.06 65.4 70.8 79.21
85.7 76.1
100.0 86.6 100.0 82.6
73.1 65.1
87.5 91.9 86.0 90.4
96.4 94.8
84.8 88.4 75.4 81.8
91.9 88.2
71.4 73.1 65.2 70.5
74.9 75.9
55.2 60.2 49.87 66.4 66.1 45.75
65.1 65.8
25.7 36.9 23.1 21.8
48.1 20.5
29.3 32.0 17.7 20.8
34.6 24.0
64.2 62.0 60.4 62.1
59.7 63.7
66.0 58.4 64.5 57.9
50.8 51.3
-------
Phase IIIB Echinoderm Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
D1 1 A 76 14 90
IB 86 17 103
2 A 117 11 128
2 B 95 15 110
D1C4 - 50/50 1 A 83 3 86
1 B 118 5 123
2 A 79 5 84
2 B 95 6 101
3 A 125 16 141
3 B 126 17 143
4 A 98 44 142
4 B 81 56 137
5 A 73 5 78
5 B 57 6 63
D1C4 25/75 1 A 78 8 86
IB 102 3 105
2 A 87 6 93
2 B 91 7 98
3 A 104 12 116
3 B 98 18 116
4 A 129 23 152
4 B 123 22 145
5 A 121 25 146
5 B 131 13 144
Page 14 of 12
i Mortality and
Abnormality
Replicate %
Mortality
Station %
Mortality
%
Abnormality
Replicate %
Abnormality
Station %
Abnormality
32.0
23.0
0.0
15.0
25.7
0.0
29.3
15.0
0.0
0.0
12.3
27.5
34.6
49.0
30.2
8.7
22.1
18.5
6.9
12.3
0.0
0.0
0.0
0.0
27.5
7.5
12.8
22.1
0.0
19.9
41.8
19.4
20.3
9.6
0.0
0.0
17.48
19.33
9 87
15.6
16.5
8.6
13.6
3.5
4.1
6.0
5.9
11.3
11.9
31.0
40.9
6.4
9.5
9.3
2.9
6.5
7.1
10.3
15.5
15.1
15.2
17.1
9.0
16.0
11.1
3.8
5.9
11.6
35.9
8.0
6.1
6.8
12.9
15.2
13.1
13.57
13.05
10.81
-------
Phase IlIB Echinoderm Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
24 HOUR UNAERATED
Seawater
Control
A
B
A
B
A
B
A
B
A
B
114
77
116
89
140
96
132
138
123
149
9
10
8
9
4
3
6
11
1
0
123
87
124
98
144
99
138
149
124
149
Ml
A
B
A
B
A
B
A
B
A
B
101
85
97
97
118
131
113
107
96
100
6
9
3
7
9
11
9
9
21
23
107
94
100
104
127
142
122
116
117
123
M1C2 - 50/50
A
B
A
B
A
B
A
B
A
B
112
158
114
116
127
123
112
123
111
87
9
9
7
12
14
16
14
7
18
7
121
167
121
128
141
139
126
130
129
94
M1C2 - 25/75
A
B
A
B
A
B
A
144
142
145
123
142
98
129
11
10
13
6
19
11
9
155
152
158
129
161
109
138
Page 15 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 82.2%
Normal = 95.1%
Abnormal = 4.9%
Final seawater control count is = 117
This number is used as 0% mortality for all
further calculations.
14.0 20.8 12.20 5.6 7.6 9.15
27.6 9.6
17.4 17.4 3.0 4.9
17.4 6.7
0.0 0.0 7.1 7.4
0.0 7.7
3.7 6.3 7.4 7.6
8.9 7.8
18.2 16.5 17.9 18.3
14.8 18.7
4.6 2.3 4.46 7.4 6.4 8.73
0.0 5.4
2.9 2.0 5.8 7.6
1.2 9.4
0.0 0.0 9.9 10.7
0.0 11.5
4.6 2.3 11.1 8.2
0.0 5.4
5.5 15.7 14.0 10.7
25.9 7.4
0 0 0.0 1.65 7.1 6.8 7.96
0.0 6.6
0.0 0.0 8.2 6.4
0.0 4.7
0.0 8.3 11.8 10.9
16.5 10.1
0.0 0.0 6.5 8.1
-------
Phase IIIB Echinoderm Larval Data
Page 16 of 12
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
% Mortality and
Abnormality
Replicate %
Mortality
Station %
Mortality
%
Abnormality
Replicate %
Abnormality
Station %
Abnormality
B
A
B
132
123
122
14
11
9
146
134
131
0.0
0.0
0.0
0.0
9.6
8.2
6.9
7.5
D1
A
B
A
B
A
B
A
B
A
B
104
115
83
98
107
76
106
145
118
112
5
9
8
5
9
10
16
19
27
22
109
124
91
103
116
86
122
164
145
134
11.4
2.0
29.3
16.5
8.9
35.3
9.7
0.0
0.0
4.6
6.7
22.9
22.1
4.9
2.3
11.77
4.6
7.3
8.8
4.9
7.8
11.6
13.1
11.6
18.6
16.4
5.9
6.8
9.7
12.4
17.5
10.46
D1C4 - 50/50
A
B
A
B
A
B
A
B
A
B
109
113
124
120
86
99
111
91
113
116
11
11
8
6
12
8
8
4
10
9
120
124
132
126
98
107
119
95
123
125
7.2
3.7
0.0
0.0
26.7
15.7
5.5
22.5
3.7
1.2
5.5
0.0
21.2
14.0
2.5
8.62
9.2
8.9
6.1
4.8
12.2
7.5
6.7
4.2
8.1
7.2
9.0
5.4
9.9
5.5
7.7
7.48
D1C4 25/75
A
B
A
B
A
B
A
B
A
B
108
112
124
140
116
139
104
109
123
127
12
10
10
7
13
9
15
11
13
7
120
122
134
147
129
148
119
120
136
134
8.0
4.6
0.0
0.0
I.2
0.0
II.4
7.2
0.0
0.0
6.3
0.0
0.6
9.3
0.0
3.24
10.0
8.2
7.5
4.8
10.1
6.1
12.6
9.2
9.6
5.2
9.1
6.1
8.1
10.9
7.4
8.31
-------
Phase II1B Echinoderm Larval Data
Station
Container
Number
Count
Total
:ep.
Rep.
Normal
Abnormal
Survivi
1
A
98
10
108
1
B
76
15
91
2
A
92
6
98
2
B
89
7
96
3
A
112
10
122
3
B
89
9
98
4
A
108
23
131
4
B
84
19
103
5
A
94
11
105
5
B
89
18
107
1
A
2
98
100
1
B
3
110
113
2
A
4
101
105
2
B
0
100
100
3
A
0
124
124
3
B
0
83
83
4
A
4
120
124
4
B
0
111
111
5
A
2
100
102
5
B
0
131
131
1
A
13
82
95
1
B
15
76
91
2
A
28
100
128
2
B
25
103
128
3
A
28
99
127
3
B
31
124
155
4
A
32
99
131
4
B
38
95
133
5
A
61
77
138
5
B
36
71
107
1
A
53
67
120
1
B
57
66
123
2
A
95
46
141
2
B
91
46
137
3
A
118
43
161
3
B
106
63
169
4
A
55
38
93
Seawater
Control
GREENBOOK
Greenbook
Ml
M1C2 - 50/50
M1C2-25/75
Page 17 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 70.5%
Normal = 87.9%
Abnormal = 12.1%
Final seawater control count is = 93
This number is used as 0% mortality for all
further calculations.
97.9 97.3 98.39 98.0 97.7 98.63
96.8 97.3
95.7 97.9 96.2 98.1
100.0 100.0
100.0 100.0 100.0 100.0
100.0 100.0
95.7 97.9 96.8 98.4
100.0 100.0
97.9 98.9 98.0 99.0
100.0 100.0
86.0 85.0 67.02 86.3 84.9 75.55
83.9 83.5
69.9 71.5 78.1 79.3
73.1 80.5
69.9 68.3 78.0 79.0
66.7 80.0
65.6 62.4 75.6 73.5
59.2 71.4
34.5 47.9 55.8 61.1
61.3 66.4
43.1 40.9 15.11 55.8 54.7 36.88
38.8 53.7
0.0 1.1 32.6 33.1
2 3 33.6
0.0 0.0 26.7 32.0
0.0 37.3
40.9 28.6 40.9 35.6
-------
Phase IIIB Echinoderm Larval Data
Container Count Total
Station Number Rep Rep. Normal Abnormal Survival
4 B 78 34 112
5 A 84 35 119
5 B 93 37 130
D1 1 A 96 43 139
1 B 110 32 142
2 A 82 36 118
2 B 90 33 123
3 A 85 30 115
3 B 88 33 121
4 A 75 23 98
4 B 77 30 107
5 A 83 16 99
5 B 64 18 82
D1C4 - 50/50 1 A 102 40 142
IB 80 27 107
2 A 60 35 95
2 B 77 43 120
3 A 73 67 140
3 B 71 51 122
4 A 107 46 153
4 B 112 61 173
5 A 101 17 118
5 B 108 27 135
D1C4 25/75 1 A 83 22 105
1 B 107 22 129
2 A 84 48 132
2 B 79 48 127
3 A 67 38 105
3 B 82 46 128
4 A 73 15 88
4 B 59 33 92
5 A 88 27 115
5 B 74 34 108
Page 18 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
16.2 30.4
9.8 4.9 29.4 28.9
0.1 28.5
0.0 0.0 10.83 30.9 26.7 25.38
0.0 22.5
11.9 7.6 30.5 28.7
3 3 26.8
8.7 7 1 26.1 26.7
5.5 27 3
19.4 18.4 23.5 25.8
17 3 28.0
10.8 21.1 16.2 19.1
31.3 22.0
0.0 7.0 11.22 28.2 26.7 31.55
14.1 25.2
35.6 26.4 36.8 36.3
17.3 35.8
21.6 22.7 47.9 44.8
23.7 41.8
0.0 0.0 30.1 32.7
0.0 35.3
0.0 0.0 14.4 17.2
0 0 20 0
10.8 5.4 15.99 21.0 19.0 29.22
0.0 17.1
9.8 12.5 36.4 37.1
15.1 37.8
28.0 20.0 36.2 36.1
11.9 35.9
21.6 29.1 17.0 26.5
36.6 35.9
5.5 13.0 23.5 27.5
20.5 31.5
-------
Phase IIIB Echinoderm Larval Data
Station
Container
Number
Rep.
Count
Rep.
Normal
Total
Abnormal Survival
GREENBOOK WITH PSDDA COUNT
Seawater
Control
GreenbookVPSDDA
A
B
A
B
A
B
A
B
A
B
102
109
104
114
125
100
114
117
107
103
22
15
7
6
17
34
17
18
11
12
124
124
111
120
142
134
131
135
118
115
Ml
A
B
A
B
A
B
A
B
A
B
0
0
0
1
0
0
2
0
1
0
16
21
26
32
3
2
21
17
17
18
16
21
26
33
3
2
23
17
18
18
M1C2 - 50/50
1
1
2
2
3
3
4
4
5
5
A
B
A
B
A
B
A
B
A
B
24
36
32
49
66
58
53
58
48
57
56
52
43
46
28
30
45
42
65
62
80
88
75
95
94
88
98
100
113
119
M1C2 -25/75
A
B
A
B
A
B
A
78
87
83
79
77
85
69
37
38
41
25
41
29
35
115
125
124
104
118
114
104
Page 19 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
Control Survival = 83.4%
Normal = 87.3%
Abnormal = 12.7%
Final seawater control count is = 110
This number is used as 0% mortality for all
further calculations.
100.0 100.0 99.63 100.0 100.0 98.27
100.0 100.0
100.0 99.5 100.0 98.5
99.1 97.0
100.0 100.0 100.0 100.0
100.0 100.0
98.2 99.1 91.3 95.7
100.0 100.0
99.1 99.5 94.4 97.2
100.0 100.0
78.1 72.6 56.07 70.0 64.5 49.63
67.1 59.1
70.8 63.0 57.3 52.9
55.3 48.4
39.7 43.4 29.8 31.9
47.0 34.1
51.6 49.3 45.9 44.0
47.0 42.0
56.2 52.1 57.5 54.8
47.9 52.1
28.8 24.7 34.98 32.2 31.3 31.23
20.5 30.4
24.2 26.0 33.1 28.6
27.9 24.0
29.7 26.0 34.7 30.1
22.4 25.4
37.0 40.2 33.7 32.4
-------
Phase 1IIB Echinoderm Larval Data
Container Count T otal
Station Number Rep. Rep. Normal Abnormal Survival
4 B 62 28 90
5 A 50 20 70
5 B 42 27 69
D1 1 A 81 11 92
1 B 66 9 75
2 A 94 12 106
2 B 76 8 84
3 A 74 26 100
3 B 71 16 87
4 A 94 15 109
4 B 111 30 141
5 A 79 10 89
5 B 75 8 83
D1C4 - 50/50 1 A 26 2 28
1 B 26 3 29
2 A 30 0 30
2 B 23 2 25
3 A 34 8 42
3 B 48 9 57
4 A 55 14 69
4 B 54 20 74
5 A 51 15 66
5 B 68 17 85
D1C4 - 25/75 1 A 17 4 21
1 B 17 7 24
2 A 39 22 61
2 B 47 18 65
3 A 43 U 54
3 B 55 12 67
4 A 37 10 47
4 B 58 11 69
5 A 24 4 28
5 B 22 5 27
Page 20 of 12
% Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnontulity Abnormality
43.4 31.1
54.3 58.0 28.6 33.9
61 6 39.1
26.0 32.9 25.16 12.0 12.0 14.51
39.7 12.0
14,2 22.4 11.3 10.4
30.6 9.5
32.4 33.8 26.0 22.2
35.2 18.4
14.2 7.1 13.8 17.5
0.0 21.3
27.9 29.7 11.2 10.4
31.5 9.6
76.3 76.3 62.10 7.1 8.7 15.04
76.3 10.3
72.6 75.8 0.0 4.0
79.0 8.0
68.9 62.6 19.0 17.4
56.2 15.8
49.8 50.2 20.3 23.7
50.7 27.0
53.4 45.7 22.7 21.4
37.9 20.0
84.5 84.5 67.21 19.0 24.1 22.03
84.5 29.2
64.4 60.7 36.1 31.9
57.1 27.7
60.7 55.3 20.4 19.1
49.8 17.9
66.2 56 6 21.3 18.6
47,0 15.9
78 1 79.0 14.3 16.4
79.9 18.5
-------
Phase DIB Echinoderm Larval Data
Station
Container
Number
Rep.
Count
R*P
Normal
Total
Abnormal Survival
CADMIUM REFERENCE TOXICANT
12.0 mg/1
1
A
0
34
34
12.0 mg/1
1
B
0
24
24
12.0 mg/1
2
A
0
20
20
12.0 mg/1
2
B
0
29
29
6.0mg/l
1
A
3
23
26
6 0mg/l
1
B
5
13
18
6.0mg/l
2
A
3
16
19
6.0mg/l
2
B
5
17
22
3.0mg/l
I
A
6
6
12
3.0mg/l
1
B
13
2
15
3 0mg/l
2
A
16
7
23
3.0mg/l
2
B
6
8
14
1.5mg/l
1
A
29
4
33
1,5mg/l
1
B
9
2
11
1,5mg/l
2
A
11
5
16
1,5mg/l
2
B
9
7
16
seawater
1
A
22
2
24
seawater
1
B
17
0
17
seawater
2
A
27
1
28
seawater
2
B
17
3
20
PHENOL REFERENCE TOXICANT
seawater
1
A
22
1
23
1
B
18
1
19
2
A
18
2
20
2
B
13
1
14
36.25
1
A
27
3
30
1
B
18
1
19
2
A
24
2
26
2
B
24
2
26
62.50
1
A
19
2
21
I
B
15
4
19
2
A
16
2
18
2
B
13
3
16
125
1
A
4
17
21
1
B
10
25
35
2
A
1
22
23
2
B
3
13
16
250
1
A
0
9
9
1
B
0
22
22
Page 21 of 12
% Mortality and
Abnormality
Replicate %
Mortality
Station %
Mortality
%
Abnormality
Replicate %
Abnormality
Station %
Abnormality
100.0
100.0
100.0
100.0
85.5
75.9
85.5
75.9
71.1
37.3
22.9
71.1
-39.8
56.6
47.0
56.6
8.33
0.00
3.57
15.00
100.0
100.0
80.7
80.7
54.2
47.0
8.4
51.8
4.2
9.3
100.00
80.72
50.60
30.12
6.73
100.0
100.0
100.0
100.0
88.5
72.2
84.2
77.3
50.0
13.3
30.4
57.1
12.1
18.2
31.3
43.8
8.3
0.0
3.6
15.0
#
#
#
0
#
#
*
0
#
#
#
if
#
100.0
100 0
80.3
80.7
31.7
43.8
15.2
37.5
4.2
9.3
100.00
80.54
37.73
26.33
6.73
4.35
5.26
10.00
7.14
-52.11
-1.41
-35.21
-35.21
-7.04
15.49
9.86
26.76
77.46
43.66
94.37
83.10
100.00
100.00
4.8
8.6
-26.8
-35.2
4.2
18.3
60.6
88.7
100.0
6.69
-30.99
11.27
74.65
100.00
4.3
5.3
10.0
7.1
10.0
5.3
7.7
7.7
9.5
21.1
11.1
18.8
81.0
71.4
95.7
81.3
100.0
100.0
4.8
8.6
7.6
7.7
15.3
14.9
76.2
88.5
100.0
6.69
7.66
15.11
82.32
100.00
-------
Phase IUB Echinoderm Larval Data
Container Count Total
Station Number Rep. Rep. Normal Abnormal Survival
2 AO 22 22
2 BO 22 22
Page 22 of 12
i Mortality and Replicate % Station % % Replicate % Station %
Abnormality Mortality Mortality Abnormality Abnormality Abnormality
100.00 100.0 100.0 100.0
100.00 100.0
-------
AMMONIA DATA
An Employee-Owned Company
-------
Phase 1116 Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
Exper Num 920817
DATE 26-Aug-92
DAY 0
Filename
2DE08171
analysis
sand dollars initiation
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
46.1
-10.7
-56.8
1
9.26
before samples
44.4
-12.0
-56.4
-56.60
2
9.27
-56.90
3
9.28
(assumes pressure of 1 atm.)
47.8
-9.4
-57.2
4
9.29
after samples
-57.20
5
9.30
6
9.32
7
9.33
Samples with mv > 139.7 were below the standard curve and
8
9.34
should serve as estimates only. 1 < 0.5 ppm)
J
f samples were not diluted, enter 1 for dilution factor
j Sample Samp
Diluent
mcl
Cone
Temp
Corrected Total Free
J Sample ml mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution NH3 (unionized)
fJar
ID read diluted
added
mv
added
w Std
ppm
C.
PPt
pH factor ppm NH3 ppm Mean
std 1.0
std 1.0
std 2.5
std 2.5
std 5.0
std 5.0
std 8.0
std 8.0
std 10.0
std 10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
119.8
119.4
97.1
97.2
79.2
78.3
66.9
67.3
62.2
61.5
10.00
1.00
0
std ppm
0.10
std mv
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.50
1.00
1.00
2.50
2.50
5.00
5.00
8.00
8.00
10.00
Ml 4a init. *
5.0
152.6
16.4
28
7.98
1.00
0.29
0.01
Ml 4ua init. *
5.0
151.7
16.2
28
7.91
1.00
0.30
0.01
Ml 24ua init.*
5.0
144.7
16.0
30
7.95
1.00
0.39
0.01
Ml G.B. init.*
5.0
126.2
16.4
28
7.97
1.00
0.80
0.02
Dl 4a *
5.0
146.3
16.8
28
7.87
1.00
0.37
0.01
Dl 4ua init.*
5.0
149.4
16.3
30
7.91
1.00
0.33
0.01
Dl 24ua init.*
5.0
152.1
16.1
30
7.92
1.00
0.30
0.01
DS G.B. init.*
5.0
119.8
16.7
28
7.92
1.00
1.03
0.02
MIC2 50 4a*
5.0
145.3
16.7
29
7.91
1.00
0.38
0.01
MIC2 50 4ua*
5.0
145.0
16.1
28
7.96
1.00
0.39
0.01
MIC2 50 24ua*
5.0
144.3
16.0
30
7.89
1.00
0.40
0.01
-------
Phase IIIB Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
MIC2 50G.B.*
5.0
101.0
16.1
28
7.86
1.00
2.13
0.04
sea H20 24ua*
5.0
156.1
16.0
29
8.09
1.00
0.25
0.01
stdl **
5.0
123.4
1.00
0.90
-
std 1.0**
5.0
123.3
1.00
0.90
-
std2.5* *
5.0
100.1
1.00
2.21
_
std 2.5**
5.0
99.1
1.00
2.29
_
std 5.0**
5.0
80.7
1.00
4.67
std 5.0**
5.0
80.6
1.00
4.69
std 8.0**
5.0
68.8
1.00
7.41
_
std 8.0**
5.0
68.5
1.00
7.49
-
std 10.0**
5.0
63.0
1.00
9.27
-
std 10.0**
5.0
63.1
1.00
9.24
_
*read 26 Aug 92 collected 25Aug 92 initiation
cut off std, 0.5ppm NH3-N, mv= 139.7
diluent = 28ppt pH meter slope = 90.4/and 90.0 for * and**
*'second set of standards taken at the end of the day
-------
Phase IIIB Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
Exper Mum
920817 DATE 27-Aug-92
DAY 0
Filename
2de08172
collected at initiation
sand dollars initiation
linearity checks
mv 1
mv 2
slope
MEAN
MEAN
1 pK
40.1
-16.0
-56.1
1
9.26
before samples
40.7
-15.2
-55.9
-56.00
2
9.27
-56.20
3
9.28
(assumes pressure of 1 atm.)
47.2
-9.2
-56.4
4
9.29
after samples
-56.40
5
9.30
6
9.32
7
9.33
Samples with mv > 133.2 were below the standard curve and should
8
9.34
only serve as estimates. (< 0.5 ppm)
If samples were not diluted, enter 1 for dilution factor
Sample Samp
Diluent
mcl
Cone
Temp
Corrected Total Free
Sample ml mcl, if
ml, if
Sample
Std
mv
Std
Degr
Salin
Dilution NH3 (unionized)
Jar
ID read diluted
added
mv
added
w Std
ppm
C.
PPt
pH factor ppm NH3 ppm Mean
std 1
std 1
std 2.5
std 2.5
std 5.0
std 5.0
std 8.0
std 8.0
std 10.0
std 10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
118.3
118.5
96.2
96.4
79.4
78.8
66.6
66.8
62.1
62.5
10.00
1.00
std ppm
0.10
std mv
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
2.50
2.50
5.00
5.00
8.00
8.00
10.00
10.00
MIC2 25 4a*
5.0
152.0
16.5
29
7.81
1.00
0.25
0.00
MIC2 25 4ua*
5.0
153.6
16.2
29
7.91
1.00
0.23
0.00
MIC2 25 24ua*
5.0
152.4
16.5
30
7.91
1.00
0.24
0.00
MIC2 25gbpsdda*
5.0
115.8
16.4
28
7.80
1.00
1.09
0.02
DIC4 50 4a*
5.0
151.9
17.0
30
7.81
1.00
0.25
0.00
DIC4 50 4ua*
5.0
151.4
16.4
30
7.92
1.00
0.25
0.01
DIC4 50 24ua*
5.0
155.2
16.8
32
7.88
1.00
0.21
0.00
DIC4 50 GB*
5.0
108.8
15.4
30
7.83
1.00
1.45
0.02
DIC4 25 4a*
5.0
140.9
16.9
30
7.80
1.00
0.39
0.01
DIC4 25 4ua*
5.0
142.4
16.5
30
7.92
1.00
0.36
0.01
DIC4 25 24ua*
5.0
144.5
16.4
30
7.88
1.00
0.33
0.01
-------
Phase 1MB Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
DIC4 25 GB* 5.0 103.3 15.2 29 7.82 1.00 1.82 0.03
* Collected 25 Aug 92 (initiation), analyzed 27 Aug 92
cut off std, 0.5ppm NG3-N mv = 133.2
diluent = 28ppt
pH meter slope = 91.0
-------
Phase IIIB Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
Exper Num
920817 DATE 28-Aug-92
DAY 2
Filename
2DE08173
analysis
collected at breakdown
sand dollars breakdown
Linearity checks
mv 1
mv 2
slope
MEAN
MEAN
I pK
48.0
-8.4
-56.4
1
9.26
before samples
47.4
-8.7
-56.1
-56.25
2
9.27
-56.13
3
9.28
47.0
-9.0
-56.0
4
9.29
after samples
-56.00
5
9.30
6
9.32
Samples with mv > 137.8 were below the standard curve and should only
7
8
9.33
9.34
(assumes pressure of 1 atm.)
serve as estimates. (< 0.5 ppml
If samples were not diluted, enter 1 for dilution factor
Sample
Samp
Diluent
mcl
Cone
Temp
Corrected
Total
Free
Sample
ml
mcl, if
ml, if
Sample
Std mv
Std
Degr
Salin
Dilution
NH3
(unionized)
Jar
ID
read
diluted
added
mv
added w Std
ppm
C.
PPt
pH factor
ppm
NH3 ppm
Mean
std 0.5
std 1
std 1
std2.5
std 2.5
std 5.0
std 5.0
std 8.0
std 8.0
std 10.0
std 10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
137.8
122.1
10.00
121.8
std ppm
std mv
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.50
1.00
1.00
2.50
2.50
5.00
5.00
8.00
8.00
10.00
10.00
Ml 4a break* 5.0
MI4ua break* 5.0
Ml 24ua break* 5.0
Ml GB break* 5.0
Dl 4a break* 5.0
Dl 4ua break* 5.0
Dl 24ua break* 5.0
Dl GB break* 5.0
MIC2 25 4a* 5.0
MIC2 25 4ua* 5.0
MIC2 25 24ua* 5.0
169.3
165.1
161.6
130.5
168.8
165.9
160.4
122.2
166.0
164.5
159.1
14.3
17.0
17.3
14.3
14.2
17.2
17.1
14.4
14.4
17.0
17.0
30
30
30
30
30
30
30
29
30
30
30
8.03
8.03
8.10
8.34
7.89
8.02
8.06
8.32
7.90
8.00
8.04
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
0.15
0.17
0.20
0.70
0.15
0.17
0.21
0.98
0.17
0.18
0.22
0.00
0.00
0.01
0.03
0.00
0.00
0.01
0.04
0.00
0.00
0.01
-------
Phase 1MB Echinoderm: Calculation of Total and Unionized Ammonia - Orion Probe Data
MIC2 25 GB*
5.0
117.5
15.9
30
8.34
1.00
1.19
0.06
sea H20 24ua*
5.0
169.7
18.0
30
8.27
1.00
0.14
0.01
MIC2 50 4a*
5.0
153.1
14.2
30
7.99
1.00
0.28
0.01
MIC2 50 4ua*
5.0
152.0
16.8
30
8.01
1.00
0.29
0.01
MIC2 50 24ua*
5.0
150.9
17.0
30
8.08
1.00
0.31
0.01
MIC2 50 GB*
5.0
105.3
14.6
30
8.32
1.00
1.95
0.08
DIC4 50 4a*
5.0
152.9
14.2
30
7.95
1.00
0.28
0.01
DIC4 50 4ua*
5.0
156.7
16.8
30
7.99
1.00
0.24
0.01
DIC4 50 24ua*
5.0
163.8
17.7
30
8.04
1.00
0.18
0.01
DIC4 50 GB*
5.0
110.9
14.0
30
8.50
1.00
1.55
0.10
DIC4 25 4a*
5.0
151.7
14.5
30
8.03
1.00
0.30
0.01
DIC4 25 4ua*
5.0
149.4
16.3
30
8.03
1.00
0.33
0.01
DIC4 25 24ua*
5.0
152.5
16.4
30
8.03
1.00
0.29
0.01
Dic4 25 GB*
5.0
106.5
14.3
30
8.43
1.00
1.85
0.10
'Collected 27Aug 92 (breakdown), analized 28 Aug 92
cut off std,0.5ppm NH3-N, mv = 137.8
diluent = 28ppt
pH meter slope = 91.9
-------
PHYSICAL MONITORING DATA
An Employee-Owned Company
-------
PHASE fflB ECHINODERM BIOASSAY PYHSICAL MONITORING DATA
Client EPA TESC 3-57
Experiment ft
Day 0 Day 1 Day 2
Date 25-Aug-92 26-Aug-92 27-Aug-92
Time 12:00 AM 12:00 AM 12:00 AM
Observer C. Sheehan ? C. Sheehan ??
Station
Temp Salinity DO pH
Temp Salinity DO pH
Temp Salinity DO pH
24-UA SW
16.0
29
7.1
18.4
29
6.7
18.0
30
6.6
8.27
24-UA Ml
16.0
30
3.4
18.2
29
4.2
17.3
30
4.5
8.10
24-UA D1
16.1
30
3.8
18.4
29
4.6
17.1
30
4.3
8.06
24-UA M1C2 - 50/
16.0
30
3.7
17.0
28.5
4.6
17.0
30
4.6
8.08
24-UA M1C2 - 25/
16.5
30
3.7
18.5
28.5
4.3
17.0
30
4.0
8.04
24-UA D1C4 - 50/
16.8
32
4.2
18.8
28.5
4.6
17.7
30
4.7
8.04
24-UA D1C4 - 25/
16.4
30
4.4
18.7
28.5
4.7
16.4
30
,4.1
8.03
4-UA SW
16.1
30
7.2
18.1
28
63
16.3
I 30
5.9
8.17
4-UA Ml
16.2
28
4.2
17.4
28
3.9
170
30
3.8
1 8.05
4-UA D1
16.3
30
5.2
18.0
28.5
4.3
17.2
30
4.5
8.02
4-UA M1C2 - 50/5
16.1
28
4.8
17.8
28
3.4
16.8
30
3.6
8.01
4-UA D1C4 - 25/7
16.5
30
5.5
17.3
29
4.8
16.3
30
4.5
8.03
4-UA MTC2?- 25/7
1 16-2
i 29
5.1
17.6
28.5 14.2
17.0
30
4.2
8.00
4-UA D1C4 - 50/5
! 16:4
30
5.4
18.4
28.5
4.6
16.8
30
4.8
7.99
4-A SW
15.8
30
7.1
14.8
28
8.3
14.2
28
8.2
8.06
4-A Ml
16.4
28
4.4
14.8
28
7.8
14.3
30
8.1
8.03
4-A D1
16.8
28
4.6
15.6
28
6.8
14.2
30
7.8
7.89
4-A M1C2 -50/50
16.7
29
4.8
14.7
28
7.8
14.2
30
8.0
7.99
4-A M1C2 -25/75
16.5
29
5.2
15.0
28
7.4
14.4
30
7.9
7.90
4-A D1C4 - 50/50
17.0
30
5.5
15.0
28
7.7
14.2
30
7.8
7.95
4-A D1C4 - 25/75
16.9
30
5.0
15.0
28
7.7
14.5
30
8.0
8.03
GB PSDDA D1
17.7
28
7
15.5
28
8
14.9
30
8
8.04
GB PSDDA SW
17.2
30
7.2
15.0
30
7.7
16.5
30
7.7
8.07
GB PSDDA M1C2
16.4
28
6.4
15.2
28
8.2
14.6
30
7.8
8.14
GB PSDDA D1C4
17.5
29
6.1
16.2
28
7.4
15.3
30
7.6
8.29
GB PSDDA D1C4
17.2
30
6.0
15.8
29.5
7.6
15.1
30
7.1
8.35
GB PSDDA Ml
17.5
28
6.1
15.0
28
7.7
14.6
30
7.8
8.29
GB PSDDA M1C2
16.2
28
5.8
14.7
29
7.3
14.3
30
8.0
8.23
GB SW
16.6
29
7.4
14.8
29
8.2
14.4
30
7.9
8.34
GBD1
16.7
28
6.3
14.7
28
7.8
14.4
27
7.9
8.32
GB Ml
16.4
28
5.4
14.8
28
7.8
14.3
30
8.0
8.34
GB M1C2 -25/75
16.1
28
6.6
15.3
28
7.9
15.9
30
7.7
8.34
GBM1C2- 50/50
16.1
28
4.0
16.1
29
7.7
14.6
30
7.4
8.32
GB D1C4 - 25/75
15.2
29
6.6
14.6
28
7.7
14.3
30
7.9
8.43
GB D1C4 - 50/50
15.4
30
5.5
14.2
29
7.6
14.0
30
7.9
8.50
CADMIUM
1.5
17.1
Not Taken
7
15.0
29
7
16.6
30
7
12
17.0
Not Taken
7
15.0
29
7
16.4
30
7
-------
REFERENCE TOXICANT
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
PIIIBEL
Cone.
Control
1.5000
3.0000
6.0000
12 . 0000
Number
Exposed
100
100
100
100
100
Number
Resp.
7
30
51
81
100
Observed
Proportion
Responding
0.0700
0.3000
0.5100
0.8100
1.0000
Adj usted
Proportion
Responding
0.0000
0.2409
0.4687
0.7940
1.0000
Predicted
Proportion
Responding
0.0778
0.1883
0.5159
0.8325
0.9705
Chi - Square Heterogeneity =
5.786
Mu
Sigma
Parameter
0.464103
0.325705
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
Spontaneous
Response Rate
3.575083
3.070261
0.077803
0.196769
0.312759
0.026534
3.189415,
2.457254,
0.025796,
3.960750)
3.683269)
0.129811)
-------
PIIIBEL
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
0.5086
0.2995
0.7296
EC 5.00
0.8479
0.5633
1.1246
EC10.00
1.1135
0.7873
1.4193
EC15.00
1.3383
0.9857
1.6629
EC50.00
2.9114
2.4740
3 . 3446
EC85.00
6. 3338
5.4459
7.6703
EC90.00
7.6125
6.4324
9.5250
EC95.00
9.9967
8.1787
13.2142
EC99.00
16.6645
12.6950
24.6826
-------
it
0+
9+
8+
7 +
6+
5+
4 +
3 +
2 +
1+
0 +
OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
o
• • • • o
• . o
EC10 EC25
_+—
EC50
_+ +—
EC75 EC90
_+_
EC99
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
IIIBEE
Number
Number
Observed
Proportion
Adjusted
Proportion
Cone.
Exposed
Resp.
Responding
Responding
Control
100
7
0.0700
0.0000
1.5000
100
26
0.2600
0.1757
3.0000
100
38
0.3800
0.3093
6.0000
100
81
0.8100
0.7883
12.0000
100
100
1.0000
1.0000
Predicted
Proportion
Responding
0.1023
0.0819
0.3944
0.8040
0.9762
Chi - Square Heterogeneity = 9.555
***************************************************************
* WARNING *
* *
* Significant heterogeneity exists. The results reported *
* for this data set may not be valid. The results should *
* be interpreted with appropriate caution. *
***************************************************************
***************************************************************
* NOTE *
* *
* Slope not significantly different from zero. *
* EC fiducial limits cannot be computed. *
***************************************************************
Mu
Sigma
0.548888
0.267789
Parameter
Estimate
Std. Err,
95% Confidence Limits
Intercept
Slope
2.950296
3.734283
0.602063
0.898412
( 0.359617,
( -0.131581,
5.540975)
7.600148)
Spontaneous
Response Rate
0.102313
0.062577
( -0.166957,
0.371582)
-------
IIIBEE
Estimated EC Values and Confidence Limits
Lower Upper
Point Cone. 95% Confidence Limits
EC 1.00
0.8432
EC 5.00
1.2835
EC10.00
1.6058
EC15.00
1.8679
EC50.00
3 . 5391
EC85.00
6.7054
EC90.00
7.7999
EC95.00
9.7583
EC99.00
14.8541
-------
IIIBEE
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4 +
3 +
2 +
1+
0+
. • o
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
EIIIBLCP
Cone.
Control
62.5000
125.0000
250.0000
Number
Exposed
100
100
100
100
Number
Resp.
7
11
75
100
Observed
Proportion
Responding
0.0700
0.1100
0.7500
1.0000
Adjusted
Proportion
Responding
0.0000
0.0402
0.7304
1.0000
Predicted
Proportion
Responding
0.0727
0.0354
0.7349
0.9989
Chi - Square Heterogeneity = 0.135
Mu = 2.019297
Sigma = 0.123662
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept -11.329186 3.247093 ( -17.693489, -4.964883)
Slope 8.086569 1.564536 ( 5.020078, 11.153060)
Spontaneous 0.072731 0.024941 ( 0.023847, 0.121614)
Response Rate
-------
EIIIBLCP
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
53 .9042
32.1919
68.3418
EC 5.00
65.4464
43 . 8786
78.8879
EC10.00
72.5792
51.7162
85.2275
EC15.00
77.8277
57.7525
89.8389
EC50.00
104.5435
90.9046
113.7170
EC85.00
140.4301
129.5848
158.9399
EC90.00
150.5852
137.8023
175.9381
EC95.00
166.9969
149.9581
205.8685
EC99.00
202.7550
174.1256
278.9503
-------
EIIIBLCP
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
probit
10+
9 +
8+
7 +
6+
5+
4 +
3 +
o.
2 +
1+
0+
_+ + + + + + +-
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING EC VALUES
Version 1.4
EIIIBECP
Cone.
Control
62.5000
125.0000
250.0000
Number
Exposed
100
100
100
100
Number
Resp.
8
15
82
100
Observed
Proportion
Responding
0.0800
0.1500
0.8200
1.0000
Adjusted
Proportion
Responding
0.0000
0.0751
0.8041
1.0000
Predicted
Proportion
Responding
0.0810
0.0726
0.8070
0.9993
Chi - Square Heterogeneity = 0.073
Mu
Sigma
Parameter
1.984603
0.129560
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
Spontaneous
Response Rate
-10.318002
7.718422
0.081027
2.314957
1.136156
0.027030
-14.855317,
5.491556,
0.028049,
-5.780686)
9.945288)
0.134006)
-------
EIIIBECP
Estimated
EC Values and
Confidence Limits
Lower
Upper
Point
Cone.
95% Confidence
Limits
EC 1.00
48.2178
33.4990
59.3188
EC 5.00
59.0866
44.4043
69.7262
EC10.00
65.8504
51.5512
76.0795
EC15.00
70.8478
56.9770
80.7444
EC50.00
96.5168
85.7901
105.2715
EC85.00
131.4858
120.7931
146.7717
EC90.00
141.4644
129.2327
160.9227
EC95.00
157.6581
142.0712
185.4256
EC99.00
193.1962
168.1592
244.0909
-------
EIIIBECP
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4 +
3 +
. . . o
2 +
1+
0+
—I 1 1 1 1 1
EC01 EC10 EC25 EC50 EC75 EC90 EC99
------- |