PB83-172163
Chemical Contaminants in Edible,
Non-Salmonid Fish and Crabs from
Commencement Bay, Washington
(U.S.) Environmental Protection Agency
Seattle, wa
Dec 82
(L3» OcprtNi if Ommm
h
-------
TECHNICAL REPORT DATA
(Mease read Instructions on the reverse before computing)
1. REPORT NO.
EPA 910/9-82-093
2.
3 PB63-172163
4. TITLE AND SUBTITLE
Chemical Qontaminants in Edible, Non-Salmaiid Fish
and Crabs frcm Ocnmencement Bay, Washington
B. RErw..
December 1982
«. PERFORMING ORGANIZATION CODE
?.^thor(s) Arnold r. uaruer, Joseph M. curmins, Joseph N.
Bl'" zpvich, Robert H. Rieck, Roy L. Arp, Carolyn E.
Garifcmark, Stephen V.W. Pope, Stan Filip
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME: AND AUD8ESS
U.S. Environmental Protection Agency Region 10
Envixbrirental Services Division laboratory
1200 6th Avenue
Seattle, WA 98101
10. PHOGHAM fcLSMENT NO.
11. CON TRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
13. TYPE OF REPORT ANO PERIOD COVERED
Final Julv 19^1 slfamV*1982
14. SPONSORING AGENCY CODE
18 SUPPLEMENTARY NOTES
16. ABSTRACT
Information was collected to provide the feccma-Pierce County Health Department with
chemical data needed to begin a preliminary assessment of airy health risk possibly
faced by persons eating fish and crabs frctn Oanmencenient Bay and adjacent waters.
Sampling was conducted frcm July 1981 to January 1982. Eighty-six ncn-salnonid fish
and six Dungeness crabs were collected from popular sport fishing locations in the
vicinity of Carmencanent Bay and frcm a remote reference area, Discovery Bay. Muscle
tissue frcm these specimens was chmeicaily analyzed far selected metals and organic
compounds (EPA priority pollutnats).
Specimens frcm the Ganroencement Bay area generally had higher contaminant levels than
did the specimens collected from Discovery Bay. The pollutant concentrations in the
fish and crab varies considerably, depending on the species and their location. The
highest concentrations of polychlorinated biphenyls (PCB's) and pesticides were
measured in fish frcm the Hybelos Waterway, whiio the highest total metal concentra-
tions measured in fish were found in flatfish frcnri the Point Defiance dock. Regard-
less of where the crabs were collected, they contained total metal concentrations
several times higher than the highest levels detected in fish.
*. DESCRIPTORS
b.lOENTIFIERS/OPEN ENDEO TERMS
c. COSATi Field/Group
Cbntaminants
Ibxicity
Chemical Analysis
Hater Pollution Effects (Animals)
Organic (impounds
Tissue Extracts
Oaxmencement Bay
Puget Sound
18. DISTRIBUTION STATEMENT
Release Unlimited
19 SECURITY CLASS (This Report)
119
30. SECURITY CLASS (TMtpf)
nuciD si
aa. price —~
CrA Farm 2220-1
NATIONAL ie<-nmv_«L
information service
"US S2SZI&V
-------
EPA-910/9-82-093
December 1982
CHEMICAL CONTAMINANTS IN EDIBLE, NON-SALMONID
FISH AND CRABS FROM COMMENCEMENT BAY, WASHINGTON
Environmental Services Division
Laboratory
U.S. Environmental Protection Agency
Region 10
Seattle* Washington
Arnold R.
Joseph M.
Joseph N.
Robert H.
Gahler
Cuonlns
Blazevlch
R1eck
Roy L. Arp
Carolyn E. Sangmark
Stephen V.W. Pope
Stan F1l1p
(*>
RXD0D00S0D3
-------
DISCLAIMER
Mention of trade names or commercial products does not
constitute endorsement or recoonendatlon for use.
H
-------
ABSTRACT
A survey of chemical contaminants 1n fish and crabs from Puget Sound+s
Commencement Bay area was Initiated following reports by various agencies
that certain waterways in the area contained relatively high levels of
potentially toxic chemicals. Information was collected to provide the
Tacoma-Pierce County Health Department with chemical data needed to begin
a preliminary assessment of any healtn risk possibly faced by persons
eating fish and crabs from Commencement Bay and adjacent waters.
Sampling was conducted from July 1981 to January 1982. E1ghty-s1x
non-salmonld fish and six Oungeness crabs were collected from popular
sport fishing locations in the vicinity of Commencement Bay and from a
remote reference area, Discovery Bay. Muscle tissue from these specimens
was chemically analyzed for selected metals and organic compounds (EPA
priority pollutants).
Specimens from the Commencement Say area generally had higher contaminant
levels than did the specimens collected from Discovery Bay. The
pollutant concentrations tn the fish and crabs varied considerably,
depending on the species and their location. The hlgnest concentrations
of polychlorlnaterJ b1phenyls (PCB's) and pesticides were measured In fish
from the Hylebos Waterway, while the highest total metal concentrations
measured In fish were found 1n flatfish from the Point Defiance Dock.
Regardless of *
-------
TABLE OF CONTENTS
Page
ABSTRACT 111
SCOPE OF STUDY 1
CONCLUSIONS 2
RECOMMENDATIONS 3
INTRODUCTION 4
METHODS 5
Sampl1ng Procedures 5
Selection of Sampling Stations 5
Collection of Samples 5
Handling and Preparation of Samples 7
Initial Handling 7
Final Preparation 8
Analytical Methods 10
Procedures for Organic Compounds 10
Procedures for Metals 10
RESULTS AND DISCUSSION 11
Polychlorinated B1phenyls (PCB's) 11
Chlorinated Butadienes (PCBD's) 14
Hexacholorobenzene (HCB) 14
Chlorinated Pesticides 14
Volatile, Acidic, and Neutral organic Compounds 14
Metals 16
1v
-------
Biological Observations
Composition and Distribution of Samples
Condition of Samples
General Conditions
Parasites
Size and Age of Samples
REFERENCES
ACKNOWLEDGEMENTS
Figure 1. Commencement Bay Sampling Sites.
Table 1. Distribution of Fishes and Crabs
Collected from Conifioncement Bay,
Adjacent Waterways, and the
Discovery Bay Reference Area.
Table 2. Average PCB Concentrations 1n F1sh
Muscle Tissue Sampled In the
Commencement Bay Area and a Reference
Station at Olscovery Bay.
Table 3. Distribution of the Bloodworm, PhUometra,
1n English Sole and Rock Sole from
the Commencement Bay Area and the
Discovery Bay Reference Station.
Table 4. Mean Lengths and Estimated Ages of
Selected Fishes Collected from the
Commencement Bay Area and the Olscovery
Bay Reference Station.
21
21
22
22
23
24
26
27
6
12
13
23
25
v
-------
APPENDIX A. Comparfslon of Concentrations of PCB's and Pesticides Among
Fish Species and Sampling Sites.
Figures A Average of Total PCB and Pesticide
1-3. Concentration Among Fishes Collected
from Four Comnencement Bay Sites and
a Reference Station at Discovery Bay. 28
Figure A-1. Bottom Fishes 28
Figure A-2. Mixed Fishes 29
Figure A-3. Off-Bottom Fishes 30
Figures A Average of Total PCB and Pesticide
4-14. Concentration 1n F1sh and Crab
Muscle Tissue Collected from Four
Conrnencetnent Bay Sites and a Reference
Station at Discovery Bay. 31
Figure A-4. English Sole 31
Figure A-5. Flathead Sole 32
Figure A-6. Rock Sole 33
Figure A-7. Starry Flounder 34
Figure A-8. Buffalo Sculpln 35
Figure A-9. Pacific Staghorn Sculpln 36
Figure A-10. Pacific Cod 37
Figure A-11. Pacific Hake 38
Figure A-12. Pacific Tomcod 39
Figure A-13. Walleye Pollock 40
Figure A-14. Dungeness Crab 41
Table A-1. Average PCB and Pesticide Concentrations
Found 1n F1sh Muscle Tissue Sampled In
the Commencement Bay Area and Discovery
Bay. 42
Table A-2. PCB and Pesticide Concentrations Found 1n F1sh
Muscle Tissue Sampled at the:
Kylebos Waterway 45
City Waterway 46
Old Town Dock 47
Point Defiance Dock 48
D1scovery Bay 49
v1
-------
Table A-3. PC8 and Pesticide Concentrations Found
1n Crab Muscle Tissue. 50
APPENDIX B. Comparison of Concentration of Metals
Among F1sh Species and Sampling Sites.
Figure B Average of Total Metal Concentration Among Fishes
1-3. Collected from Four Commencement Bay Sites and a
Reference Staiion at Discovery Bay. 51
Figure B-l. 8otto» Fishes 51
Figure B-2. Mixed Fishes 52
Figure B-3. Off-Bottom Fishes 53
Figure B Average of Total Metal Concentrations In
4-14. F1sh and Crab Muscle Tissue Collected from
Four Camnencenent Bay Sites and a Reference
Station at Discovery Bay. 54
Figure B-4. English Sole 54
Figure B-5. Flathead Sole 55
Figure B-6. Rock Sole 56
Figure B-7. Starry Flounder 57
Figure B-8. Buffalo Sculpln 58
Figure B-9. Pacific Staghorn Sculpln 59
Figure B-10. Pacific Cod 60
Figure 3-11. Pacific Hake 61
Figure B-l2. Pacific Tomcod 62
Figure B-13. Walleye Pollock 63
Figure B-l4. Dungeness Crab 64
Table B-l. Average Metal Concentrations Found 1n
F1sh and Crab Species Sampled 1n the
Coramncenent Bay Area and a Reference
Station at Discovery Bay. 65
vl 1
-------
Table B-2. Average Metal Concentrations Identified
1n F1sh Muscle Tissue Samples 1n the
Commencement Bay Area and a Reference
Station at Discovery Bay. 70
Table B-3. Metal Concentrations Found 1n F1sh Muscle Tissue
Sampled at the:
Hylebos Waterway 73
City Waterway 74
Point Defiance Dock 75
Old Town Dock 76
D1scovery Bay 77
APPENDIX C. Comparison of Organic Compounds Among Fish Species And
Sampling Sites.
Table C-l. The Average Concentrations of Priority
Pollutant Organlcs Identified 1n F1 sh
Muscle Tissues Sampled at Four Commencement
Bay Sites and a Reference Station at
Discovery Bay. 78
Table C-2. Concentratlons of Priority Pollutants Found 1n Fish
Muscle Tissue Sampled at:
Hylebos Waterway 79
City Waterway 80
Old Town Dock 81
Point Defiance Dock 82
Discovery Bay 83
Table C Concentrations of Priority Pollutants 1n
2-A. Dungeness Crab Muscle Tissue Sampled at
the Hylebos and City Waterways and
Discovery Bay. 84
Table C-3. Non-Priority Pollutant Organic Compounds Tentatively
Identified 1n F1sh Muscle Tissue Sampled at:
Hylebos Waterway 85
C1ty Waterway 88
Old Town Dock 91
Point Defiance Dock 94
D1scovery Bay 97
v111
-------
APPENDIX 0. Biological Data
Table D-l. Collection and Biological Data for F1sh
and Crab Specimens Taken In Conrnencement
and Discovery Bays. 100
APPENOIX E. Analytical Methods 109
Table E-l. Priority Pollutants 112
APPENDIX r. Quality Assurance 113
Table F-l. Quality Control Samples Analyzed. 113
Table F-2. Average Range of Duplicate Values for
Couipounds Detected In the Pesticide
Fraction. 114
Detection Limits 115
Table F-3. Detection Limits of Organic Compounds. 116
Table F-4. Detection Limits of Metals. 11C
-------
SCOPE OF STUDY
The purpose of this survey was to Identify and measure potentially toxic
metals and organic compounds In edible f1sh and crabs caught In the
Commencement Bay area. This Information was collected to assist
Tacoma-Plerce County health officials 1n determining the health risks
associated with the public's consumption of fish and crabs from
Comnencement Bay and adjacent waters.
The 114 organic compounds and 13 metals searched for during this survey
are EPA's "priority pollutants". Because polychlorlnated butadienes
(PCBO's) had previously been found 1n Hylebos Waterway sediment, they
were also searched for. The occurrences and concentrations of these
toxics in Commencement Bay f1sh and crabs were to be compared with the
levels found 1n fish and crabs from the reference station at Discovery
Bay. Discovery Bay 1s located 1n the northern Puget Sound area, and 1s
considered to be relatively free of chemical contamination.
Since the objective was primarily to search for chemicals on EPA's
priority pollutant 11st, the analytical procedures followed current EPA
practices. These practices did not provide detection levels as low as
procedures designed especially for the isolation and determination of
specific groups of compounds, like the polycycllc aromatic hydrocarbons.
Very little Is known concerning the metabolites of the priority
pollutants. Therefore, no effort was made to identify or search for them
during this survey.
-1-
-------
CONCLUSIONS
The edible muscle tissues of most of t*e fish and crabs collected
contained several potentially toxic chemical contaminants. The degree of
contamination varied considerably, depending on the species and the
location from which the specimens were collected. Specimens from the
Commencement Bay area generally had higher contaminant levels than did
specimens from the Discovery Bay reference site.
The highest total metal concentrations measured 1n fish were found 1n
"bottom" fish from the Point Defiance Dock. Total metal concentrations
1n "off-bottom" f1sh from the Commencement Bay area were similar to those
measured 1n "off-bottom" fish from the Discovery Bay reference area.
Crabs from both the Commencement Bay area and Discovery Bay contained
total metal concentrations that were substantially higher than levels
measured 1n fish from the same sites. The levels of mercury measured
were always well below the action level of 1.0 ppm established by the FDA
for fish and shellfish.
F1sh taken from the Hylebos Waterway contained the highest concentrations
of PCB's averaging approximately 0.5 ug/g wet weight, or about one-tenth
the FDA tolerance level of 5.0 ppm, and one-fourth the proposed tolerance
level of 2.0 ppm.
No chlorinated butadienes were found 1n any of the specimens at *he limit
of detection reached 1n this study. However, hexachlorobenzene (HCB) was
detected 1n fish and crabs from the Hylebos Waterway, but not 1n
specimens collected at other locations. The chlorinated hydrocarbon
pesticides DDT, ODD, and DDE were the only pesticides detected. These
compounds were found 1n all but one of the fish and crab samples
analyzed. Relatively high levels of one or more of these pesticides were
detected 1n Individual fish from both the Commencement Bay area and the
Discovery Bay reference site. However, the highest average total
pesticide level was measured 1n specimens from the Hylebos Waterway.
Specimens from Discovery Bay had the lowest average total pesticide
level. Concentrations of DDT and Its metabolites never exceeded the FDA
action level of 5.0 ppm 1n fish.
Ph .alates were commonly found 1n many of the fish. In addition, a
limited number of volatile organic compounds were detected 1n a few of
the fish and crabs collected.
Although most of the contaminants detected 1n the edible muscle tissues
appeared to be present at relatively low levels, efforts should continue
to be directed at assessing their human health significance. The
potential toxicity of combinations of these and other contaminants and
their metabolites must be considered 1n the risk assessment process.
-2-
-------
RECOMMENDATIONS
The data should be reviewed and statistically analyzed by health
officials and toxlcologlsts to assess the possible human health effects
of individual and total contaminant levels 1n fish and crabs from the
Comnencement Bay area.
The presence of pentachloropropene as well as other unidentified
halogenated compounds found 1n the Hylebos Waterway samples should be
Investigated further. "Off-bottom" fish from the Hylebos should be
analyzed to determine whether pentachloropropene and the unidentified
halogenated compounds are present 1n these fish. It will be necessary to
obtain some of the propene derivative to further verify the mass spectrum.
While the sample size of the Individual species caught was often small,
the sample size of the broader categories, e.g., the "bottom" fish, may
allow further statistical analyses to be performed. Hopefully, this
could provide additional general Information about contaminant uptake 1n
these fish.
-3-
-------
INTRODUCTION
The potential for Puget Sound and adjacent waters to be adversely
affected by expanding urban and Industrial development has been known for
some time. However, the possible extent and nature of some of these
effects were not recognl2ed until recently. In 1980, the National
Oceanic and Atmospheric Administration (NOAA), Office of Marine Pollution
Assessment (OMPA), reported on studies which described the distribution
and possible biological effects of selected chemical contaminants 1n
Puget Sound (6,11). Water, suspended matter, sediments, and aquatic
organisms collected from several locations 1n Puget Sound wcro found to
have measureable concentrations of one or more of the following groups of
chemicals: metals, polychlorlnated b1phenyls (PCB's), petroleum
hydrocarbons, and chlorinated organic compounds. Selected waterways 1n
the Commencement Bay area generally appeared to be the most polluted.
In addition to the chemical contaminants observed, some of the fish,
crabs, shrimp, and clams collected from a few of the contaminated areas
w-ve found by Mallns and his associates (6) to have a variety of lesions
of the liver or other organs. These observations raised questions about
not only the relationship between the chemical contaminants and the
health of associated aquatic organisms, but also about the possible
influence of these chemicals on the health of those persons eating the
contaminated seafoods. Considering the general lack of chemical and
toxlcologlcal Information on the chemical contaminants present 1n edible
portions of aquatic organisms Inhabiting the Commencement Bay area, the
Tacoma-Plerce County Health Department Issued warnings advising people
not to eat fish and shellfish caught 1n certain waterways. Since this
work has been completed, Mallns and his associates (7) have published
chemical and biological data on livers taken from 85 fish throughout
Puget Sound. They presented data on organic compounds 1n liver and
muscle tissue from 13 fish caught 1n Commencement Bay. These Included
five English sole, five salmon, and three cod.
To provide more complete information on which to assess the human health
significance of the chemical contamination described, EPA Region 10
Initiated a chemical survey 1n July 1981, to determine 1f potentially
toxic organic compounds and metals were present 1n edible muscle tissues
of non-salmonld fish and crabs from Commencement Bay and nearby waters.
The synergistic or antagonistic effects of the contaminants and their
metabolites found 1n the fish are generally unknown at the present time.
The effect of the cooking methods used by the public was not considered
1n this report.
-------
METHODS
SAMPLING PROCEDURES
Because the results of this survey were to be used to help assess
possible risks to human health, 1t Mas essential that samples be
collected 1n a representative manner, and not chemically contaminated as
a result of handling. The various phases of the sampling program are
discussed below.
Selection of Sampling Stations
F1sh and crab samples were collected for chemical analysis from several
stations located 1n Commencement Bay and connecting waterways as well as
from a reference area. Discovery Bay (Figure 1). The stations
established 1n the Commencement Bay area were as follows:
1. Hylebos Waterway (Northwest of the 11th St. Bridge)
2. City Waterway (Totem Marina Dock)
3. Old Town Dock
4. Point Defiance Dock
Hylebos Waterway was chosen as a sampling site because of the relatively
high concentrations of organic compounds that previously had been
detected 1n water, sediments,and aquatic organisms collected from that
location (6,11). City Waterway (Totem Marina Dock), Old Town Dock, and
the Point Defiance Dock were selected because of their popularity as
public sport fishing sites (Personal Communication, Doug Pierce,
Tacoma-Plerce County Health Department) as well as the representative
coverage of the study area they provided. Crabs from the Commencement
Bay area were collected only from popular sport crabbing locations - the
Hylebos and City Waterways.
The selection of Discovery Bay as the reference, or background, area was
based on considerations of Its relative Isolation from the more
Industrialized and developed areas of Puget Sound and the Strait of Juan
de Fuca.
Collection of Samples
The 86 fish and 6 Dungeness crabs obtained 1n conjunction with this study
were collected during the period from July IS, 1981 through January 7,
1982. Sampling at the Commencement Bay and waterway stations was
conducted both during the day and at night using some of the same fishing
techniques as those being used by sport fishermen 1n the area. Samples
collected during the day were captured by hook and line as well as by an
18-foot beam trawl. Nighttime sampling was done with hook and line
only. All but one of the samples collected from the Discovery Bay
reference area were captured by commercial bottom trawlers fishing the
northern and central portions of the Bay. One fish sample was collected
during the day from the southern portion of the reference area using an
18-foot beam trawl.
-5-
-------
TACOMA. wawimjton
COMMBNCBMBNT
DAY
Mgun I.
SAMPLING
STATIONS
-6-
-------
in conjunction with this survey, the Tacoma-P1erce County Health
Department conducted a fish and crustaceancatch consumption study In the
Commencement Bay area (10). Pacific hake and walleye pollock comprised
69.3X of the catch on a weight basis, with Pacific cod and Pacific tomcod
contributing an additional 10.11. The balance of the sport catch was
made up of 33 species of fish that Included members of such groups as the
rockflsh, flatfish, and perch.
As a result of these findings, sampling efforts were primarily directed
at collecting representative numbers of two groups of non»salmon1d fish
distinguishable by their habitats and their general feeding habits:
1. Off-Bottom Fish Those fish species spending most of their
lives in the water column above the bottom,
but not necessarily 1n surface or
near-surface waters, I.e., the codflshss -
walleye pollock, Pacific hake, pacinc cod,
and Pacific tomcod, and;
2. Bottom Fish Those fish species living most of their
lives In direct contact with the bottom.
I.e., the flatfishes - English sole, rock
sole, C-Oso'e, riatnead sole, and starry
flounder.
3. Mixed Fish During the course of sampling, several other
species of fish were collected that did not
belong to either of these groups, I.e.,
Pacific staghorn sculpln, buffalo sculpln,
rockflsh, and whltespotted greenllng.
Because of the relatively small numbers of
nrot of these fishes that were taken from
the Commencement Bay area, they were
combined Into one group rather than
establishing three or four additional
groups. For purposes of discussion, this
group was termed "mixed" fish.
Handling and Preparation of Samples
Preparation of the fish and crab samples for chemical analysis Involved a
number of steps ranging from Identifying the species collected to
filleting or picking out the edible muscle tissues of Interest. During
each of these steps, emphasis was placed on preventing the samples from
becoming chemically contaminated.
Initial Handling
As soon as possible after collection, the samples were wrapped 1n
clean aluminum foil (Note 1) and placed In an Ice chest containing
several sealed polyethylene containers of 1ce. Upon returning to the
laboratory, the samples were removed from the Ice chest and stored at
1*C until the final phase of sample preparation could be Initiated
(Note 2). The only exception to this procedure Involved the crab
samples collected from the Hyltbos *nd City Waterways. These samples
were refrigerated at 1*C for several days then stored at -12"c for
five months before thawing for final preparation.
-7-
-------
Final Preparation
Before removing any tissues for analysis, each specimen was
identified, weighed, measured, and Inspected for grossly visible
lesions, parasites, and other features of possible significance.
F1sh samples were first skinned (Note 3), then edible muscle tissue
(the fillet) was removed from both sides of each specimen using
stainless steel scalpels, scissors, and forceps (Note 4). As much
edible muscle tissue as possible was picked from the crab samples
using the same stainless steel Instruments. The tissues collected
were cut or torn Into small pieces before being placed 1n glass jars
having teflon-lined screw caps (Note 5), then weighed and stored at
1*C until processing for chemical analysis was begun.
Following the collection of the muscle tissues, fish 11vers were
removed with stainless steel scissors and forceps, placed 1n glass
jars fitted with aluminum foll-Hned screw caps (Notes 1,5), and
stored at -12*C for possible future chemical analysis. All the
livers were weighed and visually Inspected before being frozen.
Finally, the sex of the fish was confirmed by Inspection of the
gonads under a microscope, and otoliths were collected to provide
Information on the ages of the fish collected.
The gross visual Inspection of the fish and livers for such things as
lesions and parasites was Included 1n the sampling procedures to
compliment the chemical Information being collected. These general
observations were not Intended to constitute an Independent
Investigation of biological abnormalities. However, the observations
were considered appropriate 1n light of recent studies Identifying
possible links between chemical contamination and diseases of fish
and crustaceans from certain Puget Sound waters (6,7).
Notes: 1. The aluminum foil, used to line the tissue sample jar
11ds, and to wrap the whole fish and crabs, was
cleaned 1n the following manner:
a. Rinsed twice with pest1c1de-grade acetone.
b. Rinsed twice with pesticide-grade methylene
chloride.
c. Dried 10 minutes 1n a drying oven at 100*C.
2. The decision to preserve the samples for analysis by
refrigeration rather than freezing was based on
concerns about the poorly defined effects of freezing,
coupled with possible thawing and refreezlng, on the
concentrations of volatile organic compounds 1n edible
muscle tissues. The procedure or analyzing
refrigerated tissues was also believed to be
consistent with the way 1n which many of the sport
fish catches were preserved before being prepared for
consumption.
-------
3. Preliminary results of a survey of sport fishermen
Indicated that most of the non-salmonld fish caught In
the Commencement Bay area were skinned either before
or after being cooked, and the skins discarded
(Personal Communication, Don Novlello, Tacoma-Pierce
County Health Department). Consequently, the
procedure of analyzing samples of muscle tissue that
did not Include the attached skin was adopted 1n an
effort to provide the most realistic Information
possible on the chemicals actually being consumed by
the public.
4. To prevent cross-contamination, all of the Instruments
used to collect the tissues of Interest received the
following cleaning treatment between the preparation
of each sample:
a. Washed with Uqul-ltox detergent, rinsed with tap
water, then rinsed at least three times with
d1stllled-da»1neral1zed water.
b. Rinsed twice with acetone.
c. Rinsed twice with methylene chloride.
d. Dried for at least 10 minutes at 100*C and
allowed to cool before using.
5. The glass jars and teflon lid liners, used for the
storage of muscle and liver tissues, were cleaned
using the following procedure:
a.
Washed with hot water and detergent.
b.
Rinsed with tap water.
c.
Rinsed with distilled water.
d.
Dried overnight 1n a drying oven at 350*C.
e.
Rinsed with pesticide-grade acetone.
f.
Rinsed with pestlclde-grade methylene chloride
9*
Air dried.
The last rinses of acetone methylene chloride were
composited, concentrated, and checked for Impurities
by gas chromotography/electron c&pture (6C/EC).
-9-
-------
ANALYTICAL METHODS
F1sh and crab samples were analyzed for the priority pollutant organic
compounds and metals, which are listed 1n Table E-l. Additional metals
and organic compounds previously detected 1n Commencement Bay by other
Investigators were Included. Tentative Identifications were made for
those compounds showing response 1n the gas chromatography/mass
spectrometer (6C/MS) chromatograms.
Analytical methods for the priority pollutants, other organic compounds,
and metals, followed EPA procedures as much as possible. However,
modifications were needed to remove 1nterferences so that lower detection
limits were achievable. This section briefly outlines the procedures
followed. Details of the procedures and detection limits are Indicated
1n Tables F-3 and F-4.
Procedures for Organic Compounds
The edible muscle tissues of th6 fish and crab samples were extracted
Into a methylene chloride/tethanol mixture three times with a Brliikman
Polytron homogenizer. After filtering, the extracts were reduced 1n
volume and combined with organic-free water at a pH of s2. The
water/extract mixtures were extracted again with methylene chloride,
reduced In volume and divided for pesticide/chlorinated hydrocarbon
analysis and acid/neutral (A/N) analysis.
The fractions used for pesticide, PCB, andpoly chlorinated butadiene
(PC8D) analyses were further cleaned on a Florlsll column; pentane was
used to elute the PC80, PCS, and some of the chlorinated pesticide
compounds. Mixtures of diethylether/pentane were used to elute the other
pesticides. These fractions were analyzed by GC/EC
(gas chromatography/electron capture) with dual column confirmation.
Mhen the concentrations of chlorinated species were sufficiently high,
confirmation was obtained by a halogen - specific detector and/or fused
silica capillary chromatography/mass spectrometry/data system
(FSCC/MS/OS). The A/N fraction was further cleaned by gel permeation
chromatography before FSCC/MS/DS analysis. Volatile organic compounds
were analyzed by the gas purge, 6C/MS procedure is modified by the Region
10 Laboratory (3). A detailed description of the analytlal methods Is
presented 1n Appendix E.
Procedures for Metals
Samples of tissues were digested with a mixture of nitric acid and
hydrogen peroxide until the tissue was destroyed. The metals were
determined by atomic absorption spectrometry using a graphite furnace or
flame following EPA methods (8). Mercury was determined by the manual
cold vapor technique, similar to that described by EPA Method 246.5 (8).
-10-
-------
RESULTS AND DISCUSSION
A total of 86 fish and 6 Dungeness crabs were collected for chemical
analysis from the Commencement Bay stations and Discovery Bay.
The species composition of the fish and crab samples collected from each
station 1s shown In Table 1. Other biological data such as age and
physical measurements are tabulated 1n Table 0-1.
Data for metals were obtained on all the fish and crab samples. All
samples were analyzed for the volatile priority pollutants by GC/MS and
scanned for th® halogenated organic compounds by gas chromatography.
Acid and neutral priority pollutants and additional organic compounds
were determined 1n 49 samples. Results are discussed 1n terms of classes
of compounds and metals found to be present 1n relationship to the
numbers and types of fish taken from the various locations. Data are
tabulated and presented 1n graphs 1n Appendices A through D. All
averages are arithmetic means.
Polychlorlnated B1phenyls (PCB's)
Aroclor 1254 was the only PCB measured 1n significant concentrations.
PCB's were detected 1n every specimen analyzed. The range was from less
than 0.010 micrograms per gram (ug/g) for most samples from Discovery Bay
to 1.12 ug/g for a whitespotted greenllng from the Hylebos Waterway.
These concentrations are well below the 5.0 ppm tolerance for PCB's 1n
the edible portion of fish and shellfish, as set by the U.S. Food and
Drug Administration (FDA) (12).
The highest average concentration of PCB's at 0.55 and 0.62 ug/g wet
weight were found 1n "bottom" and ''mixed" fish respectively, taken from
the Hylebos Waterway (Table 2). This was confirmed by GC/MS. Average
PCB levels In "bottom" fish were from 2 to 4 times greater than the PCB
concentrationsof the "off-bottom" fish at all of the stations 1n
Commencement Bay. Unfortunately, no "off-bottom" fish were obtained from
the Hylebos Waterway. However, PCB levels 1n "mixed" fish such as
Pacific staghorn sculpln, buffalo sculpln, whitespotted greenllng, and
rockflsh were highest 1n the Hylebos Waterway at an average of 0.62
ug/g. The PCS level was lowest, less than 0.010 ug/g, 1n the fish from
the Discovery Bay reference station.
PCB levels 1n the "off-bottom" fish, such Pacific hake. Pacific tomcod,
Pacific cod, and walleye pol-ock, were similarly low for all sampled
stations, which may reflect ».ic greater migratory tendency of these fish
(Table 2). These "off-bottom" fish were not sampled in the Hylebos
Waterway. However, PCB levels 1n the whltespotted greenllng at this
station were found to be the highest of any smples taken.
PCB levels 1n Dungeness crabs were much higher in samples taken from the
Hylebos Waterway [0.052 ug/g) and City Waterway (0.068 ug/g) than 1n
those taken from Discovery Bay (less than 0.010 ug/g). The PCB levels 1n
the crab tissues were substantially lower than the levels 1n fish tissues
from the same stations.
-------
Table 1, DitfrltniHo* of Flthas at* Crabs 1ram <
Cfaiifniif- Bay.AdJacaot Vitwutyt, i
July 19, (982 - January T, 1962
nd tha Olacovary Bay Rafaranca V»a
SpMlM
Hrlaboa aatarvar
nry Mranwr via lown
(Tot** Harlaa Dock) Dock.
Holnt UaTlsnca uiscovary Bay
Dock (Rafaranc* Araa)
'oral*
V*lt«y« Mlock
Tfcaraara dulennm
9 5
9 5
20
Pacific War
Narlacclw wodW.hu
9 5
10
Pacific Tgnrf
Micro—hxkIvs
J
r
4
Pacific Cod
Mm nctocvMw
2 1
3
e«0i itk s»i%
Paraofcrva tnrtafas
5
9 J
} 5
21
Mock Sol*
UtUopidt« bl 1 laaata
3
9
FlatWad Sol*
Hippoaloaaoldaa aiaaaodoa
1 1
2
Sftrry Ftamdar
Pl at Iciitlivi stall ahts
1
1
2
C-0 Sola
^IwrMlcMn rw—nil
1
1
Pacific Staffer* Sea It la
UftoooHia mwmlaa
9
1
5 5
12
M»lf%»offa< eraaall*
Hmtam mmut stvllarf
3
3
Baffato Scalpla
twh >m fclaaa
I
I
2
*><*11*
Sabflstaa aft.
t
1
nmafaaaM Qrak
CaaOar Bag)star
2
2
2
«
teefc Q-ah
Caaear iimOctw
t
t
Milt
O
22 20
17 21
M
-------
. Average Kl Concentrations*
-------
The average PCB corcentratlons in the various species of f1sh at the
various sampling sites are depicted 1n Figures A-l through A-14.
Specific data are shown 1n Tables A-l through A-3.
Chlorinated Butadienes (PCBD's)
Detectable levels of polychlorlnated butadienes were not found 1n any of
the muscle tissues analyzed. The limit of detection was 0.001 ug/g.
Hexachlorobenzene fHCB)
Hexachlorobenzene was detected only 1n fish and crab muscle tissue
samples taken from the Hylebos Waterway. The HCB levels 1n the fish
ranged from 0.014 ug/g to 0.15 ug/g with an average of 0.078 ug/g which
was 2 to 6 times higher than in crab. The limit of detection for HCB by
GC/EC was 0.001 ug/g. The presence of HCB 1n Hylebos Waterway samples
was confirmed both with a halogen-specific detector and GC/MS.
Chlorinated Pesticides
Although a search was made for all of the chlorinated hydrocarbon
pesticides listed 1n Table E-l, the only pesticides found were DDT, DDD
and DOE. These three compounds were detected 1n all but one of the
samples of f1sh and crab muscle tissue analyzed. Total pesticide levels
(sum of the concentrations of DDT, DDD. and DDE) ranged from less than
0.001 ug/g to 0.059 ug/g, and were much lower than the PCB concentrations
measured (Tables A-l, A-2, and A-3). The highest average total pesticide
level was measured 1n species from the Hylebos Waterway, with specimens
from Discovery Bay having the lowest average total pesticide level.
Concentrations of DOT and Its metabolites were always well below the F0A
action level of 5.0 ppm.
volatile. Acidic, and Neutral Organic Compounds
A variety of volatile, acidic, and neutral organic compounds were found
by GC/MS 1n fish and crab muscle tissues taken from the five sampling
stations. Although attempts were made to characterize several hundred
compounds detected 1n tissue extracts by mass spectral matching
techniques, only 56 compounds were Identified. Compar1s1on of retention
times and mass spectra data generated from authentic substances was
possible for 17 compounds found on the priority pollutant 11st. Tentative
Identifications based on mass spectral matching results were assigned to
the remaining 43 substances. Both tetrachloroethylene and
trlchloroethylene were detected 1n fish tissue sampled at the Hylebos
Waterway. Both chemicals were detected 1n water from the Hylebos 1n
previous EPA surveys. This may be the first time volatile organlcs have
been shown to accumulate 1n f1sh. The results of 6C/MS analyses are
compiled in Tables C-1, C-2, and C-3.
Several other unidentified chlorinated substances were present 1n all
Hylebos Waterway tissue samples. These peaks were not present 1n samples
taken from other stations.
-14-
-------
Analysis of extracts o* composited Hylebos English sole and crab by
GC/halogen-specific detector and GC/MS confirmed the presence of
chlorinated compounds. One unknown compound produced a mass spectrum
similar to 1. 2, 3, 3-tetrachloro, -1-propene and another, a maiS
spectrum similar to that expected for pentachloropropene which is a
strong mutagen (9). This latter mass spectrum was similar to that of the
pentachl oropropene detected in particulate extracts from Hylebos Waterway
by Riley and associates (11). P.eanalysis of an extract of an inoustrlal
effluent discharged to the Hylebos in 1979 showed the presence of a
compound with the same mass spectrun< and relative retention time as a
chlorinated compound tentatively Identified as pentachloropropene. This
same tentatively identified compound was detected 1n fish collected from
the Hyebos Waterway.
If the compound detected 1n the fish tissue, water, and particulate
samples is pentachloropropene, an important link between discharge and
biological uptake of a hazardous substance may be indicated.
-------
Metals
The fish and crab tissue samples were analyzed for 13 metals. Arsenic,
cadmium, chromium, copper, lead, mercury, nickel, selenium, silver, and
zinc were found 1n low to moderate concentrations. Antimony, beryllium
and thallium were not detected. All concentrations are presented on a
wet weight basis.
The highest average total metal concentrations were found in the tissues
of the "bottom" fish and the "mixed" fish. T!ie "off-bottom" fish had
lower average total metal concentrations regardless of station location.
"Bottom" fish had the highest average total metal concentration at the
Point Defiance Dock station (27.6 ug/g). The average total metal
concentrations 1n "bottom" fish at the other four stations are arrayed as
follows: Hylebos Waterway 12.3 ug/g, Old Town Dock 11.4 ug/g, City
Waterway 11.1 ug/g and Discovery Bay 9.4 ug/g (Figure B-1).
While "mixed" fish contained higher average total metal concentrations
than did the "off-bottom" fish, there were no remarkable differences in
"mixed" fish averages between stations. The highest average In this
"mixed" fish category was 1n fish from the Old Town Dock area (12.8
ug/g). The second highest average was 1n fish from the Point Defiance
Dock area (12.0 ug/g). The lowest averages 1n this group were found In
fish from the Hylebos Waterway and Discovery Bay. "Mixed" fish from both
of these areas had average total metal concentrations of 10.0 ug/g. No
"mixed" fish were sampled at the City Waterway (Figure B-2).
The "off-bottom" fish had the lowest average total metal concentrations,
and the smallest differences between these values at the different
stations. "Off-bottom" fish from the Point Defiance Dock area actually
contained less total metals, (8.4 ug/g) than did "off-bottom" fish from
the reference station (8.5 ug/g). "Off-bottom" fish from the Old Town
Dock area averaged 8.0 ug/g, and "off-bottom" fish from the City Waterway
averaged 7.6 ug/g. Unfortunately no "off-bottom" fish from the Hylebos
Waterway were sampled (Figure B-3).
Crab muscle tissues contained several times more total metal than the
fish muscle tissues from the same sampling stations. Differences between
station averages were small. The reference station Dungeness crab
contained only slightly less total metal than did crab from the Hylebos
Waterway (Figure B-14).
Total and Individual metal concentrations found 1n the various fish and
crab species are presented graphically in Figures B-4 through B-14.
Averages of metal concentrations by species are compared m Table B-l and
the means and ranges by station are tabulated 1n Table B-2. Data
describing metal concentrations for Individual fish and crabs are
presented 1n Table B-3.
The different metals are discussed Individually with respect to station,
species, and fish category occurrences.
16-
-------
(Fish Tissue)
Arsenic
Arsenic appeared 1n fish and crab tissues from all stations sampled.
F1sh containing relatively high levels of arsenic were caught near
the Point Defiance Dock and at Discovery Bay.
English sole caught near the Point Defiance Dock averaged 8.6 ug/g
arsenic. Rock sole caught at the same station averaged 16.2 ug/g.
Five Pacific staghorn sculpins caught at Discovery Bay averaged 2.0
ug/g arsenic. A Pacific tomcod from Discovery Bay contained 3.4 ug/g
arsenic.
Cadmium
Concentrations of cadmium in fish tissues were low from all sampling
stations, usually below 0.01 ug/g. The Hylebos and City Waterways
produced fish containing the highest levels. Fish from the Point
Defiance Dock a*ea and Discovery Bay contained the least cadmium.
All three Pacific staghorn sculpins caught 1n the Hylebos Waterway
contained cadmium levels greater than 0.01 ug/g. The average cadmium
level in these fish was 0.021 ug/g. Five of the eleven fish caught
at the Hylebo; Waterway contained more than 0.01 ug/g cadmium.
All "off-bottom" f1sh sampled from the City Waterway, with the
exception of one Pacific tomcod, contained levels of cadmium greater
than 0.01 ug/g. The average concentration 1n these "off-bottom" fish
was 0.014 ug/g. "Bottom" fish caught in the City Waterway always
contained less than 0.01 ug/g cadmium.
Chromium
Chromium levels were generally low 1n fish tissue from all stations.
Stations producing fish with the highest concentrations of chromium
were: the Hylebos Waterway where 64 X of the fish contained
concentrations greater than 0.2 ug/g, the City Waterway were 41 X of
the f1sh contained greater than or equal to 0.2 ug/g, and the Point
Defiance Dock area where 38 % of the fish contained greater than or
equal to 0.2 ug/g. The stations producing fish with the lowest
levels of chromium were the Old Town Dock area and Discovery Bay.
At the Hylebos Waterway, all the "mixed" fish and one English sole
contained chromium levels 0.2 ug/g. The "mixed" fish there averaged
0.41 ug/g chromium.
The "bottom" fish and two "off-bottom" fish from the City Waterway
contained chromium levelsz 0.2 ug/g. "Bottom" fish from the City
Waterway contained an average of 0.33 ug/g chromium.
At the Point Defiance Dock area, "bottom" fish contained a higher
average level of chromium (0.25 ug/g) than did the "mixed" fish (0.13
ug/g) or the "off-bottom" f1sh (0.11 ug/g).
-17-
-------
Copper
Copper concentrations were relatively high 1n fish from the Point
Defiance Dock area and the Old Town Dock area. F1sh from Discovery
Bay, and the Hylebos and City Waterways contained less copper.
"Off-bottom" fish from the Point Defiance Dock area contained the
most copper of any group of fish from any station. Copper
concentrations 1n these fish averaged 0.82 ug/g. "Mixed" fish from
the Point Defiance Dock area had the second highest average
concentration at 0.76 ug/g. "Mixed" f1sh from the Old Town Dock
area had the third highest average concentration of copper a': 0.6
ug/g. followed by "off-bottom" f1sh from that same station with an
average of 0.52 ug/g.
"Mixed" and "off-bottom" f1sh consistently contained more copper on
the average than the "bottom" fish collected from any of the sampling
stations.
Lead
Lead levels were highest 1n fish caught 1n the Point Defiance Dock
area, the Old Town Dock area, and the City Waterway. Lead levels
were lower 1n fish from Discovery Bay and the Hylebos Waterway.
"Bottom" f1sh from the Point Defiance Dock area contained the highest
average lead concentration at 1.98 ug/g. This average Includes a
concentration of 10.4 ug/g lead found 1n an English sole. If this
sole were excluded as an outlier from the averaging, the average for
this group would be 0.58 ug/g lead. The second highest lead
concentrations were found 1n the "mixed" fish from the Old Town Oock
area. They averaged 0.63 ug/g lead. "Off-bottom" fish from the
Point Defiance Dock station were third highest 1n lead concentration
with an average of 0.61 ug/g.
Average levels of lead detected 1n the Hylebos Waterway samples were
actually lower than those averages found 1n Discovery Bay samples.
Mercury
The Hylebos Waterway and the Old Town Dock area produced fish with
the highest average mercury concentrations. "Off-bottom" f1sh at
Discovery Bay also contained relatively high concentrations.
The highest concentrations of mercury were found 1n wMtespotted
greenllng caught 1n the Hylebos Waterway. The average mercury
content of these fish was 0.17 ug/g. The second highest
concentrations were found 1n "off-bottom" fish from the Old Town Oock
area. These fish averaged 0.010 ug/g mercury. Walleye pollock
caught at the Discovery Bay station also contained higher mercury
concentrations with an average of 0.008 ug/g.
At all stations, where comparisons could be made, the "mixed" fish
and the "off-bottom" fish contained higher average levels of mercury
than the "bottom" fish. Levels of mercury measured were always below
the action level of 1.0 ppm established by the FDA for fish and
shellfish.
-18-
-------
Nickel
Nickel concentrations were highest in fish from the Hylebos and City
Waterways. Fish caught in Discovery Bay had the lowest average
nickel concentration with the following exception. The Hylebos
Waterway "mixed" fish had a lower average nickel concentration (0.17
ug/g) than "mixed" fish from Discovery Bay (0.26 ug/g).
English sole from the Hylebos Waterway contained consistently high
nickel concentrations, relative to other f1sh from other stations.
The average nickel concentration among these fish was 1.26 ug/g. The
second highest concentration was found among English sole from the
City Waterway. These fish averaged 0.65 ug/g nickel.
The "bottom" fish sampled always contained average nickel levels
lower than did the "mixed" fish or the "off-bottom" fish, regardless
of station.
Selenium
Selenium concentrations were low at all stations. The stations
producing f1sh with quantifiable values of any interest were the
Hylebos Waterway, the Point Defiance Dock area, and the Ola Town Dock
area.
An English sole from the Hylebos Waterway contained 0.38 ug/g
selenium. This was the highest selenium value found 1n any of the
fish. "Bottom" fish from the Point Defiance Dock area contained an
average selenium concentration of 0.17 ug/g. "Mixed" and "bottom"
fish from the Old Town Dock area contained selenium averages of
0.11 ug/g and 0.10 ug/g, respectively.
Silver
There was only one quantifiable silver value (0.009 ug/g) and this
value was lower than the silver detection limit in most cases (0.01
ug/g).
Z1nc
The highest zinc concentrations were found 1n fish from the Old Town
Dock and the Point Defiance Dock areas. Discovery Bay produced
"bottom" and "mixed" fish with lower average zinc concentrations than
any of the other sampling stations.
"Mixed" f1sh from the Old Town Dock area contained the highest
average zinc concentration at 10.27 ug/g. The second highest average
zinc concentration was found in "mixed" fish from the Point Defiance
Dock area (8.68 ug/g). "Bottom" fish from Point Defiance followed
with an average zinc concentration of 8.24 ug/g.
"Off-bottom" fish contained approximately the same concentrations of
zinc regardless of station. These f1sh also contained less zinc on
the average than "mixed" fish at any sampling station.
-19-
-------
(Crab Tissue)
Metal concentrations detected 1n Dungeness crabs, caught In the
Hylebos and City Waterways and Discovery Bay, were generally high.
Z1nc, copper, nickel, and silver concentrations were especially high.
Z1nc concentrations were consistently high 1n crabs from all stations
(49.3 ug/g to 52.6 ug/g). These averages are c to 12 times higher
than zinc concentrations found 1n the fish tissue. Commencement Bay
crabs contained 3 to 4 times the copper (12.7 ug/g to 15.0 ug/g) that
Discovery Bay crabs contained (4.3 ug/g).
Lead concentrations 1n crabs from Commencement Bay were twice as high
(0.69 ug/g to 0.82 ug/g) as those found 1n Discovery Bay crabs (0.36
ug/g). Mercury and cadmium levels 1n crabs from Commencement Bay
were similar to levels found 1n crabs from the reference station.
Arsenic and nickel concentrations 1n crabs from the Commencement Bay
area were actually lower than concentrations detected 1n crabs from
the reference station.
-20-
-------
BIOLOGICAL OBSERVATIONS
Composition and Distribution of Samples
Although emphasis was placed on collecting representative numbers of
flatfish and codfish, almost every specimen captured from the
Commencement Bay stations, up to a limit of five specimens of each
species of fish and two specimens of each species of crab per station,
was kept for analysis. Consequently, Table 1 1s Indicative of the fish
and crab species caught most frequently at these stations frcm late
sumner to mid-winter. English sole was the fish caught most frequently,
with a total of lo fish being taken by trawling from the four
Commencement Bay stations. Walleye pollock, Pacific hake, ana Pacific
staghorn sculplns were the second, third, and fourth most comnonly caught
fishes, respectively. A total of 15 pollock, 10 hake, and 7 sculplns
were caught by hook and line from four stations, with the pollock and
hake being caught only at night. Dungeness crabs were captured by
trawling from only the Hylebos Waterway and City Waterway stations. No
effort was made to catch crabs from elsewhere 1n the Commencement Bay
area.
The fact that no codfishes (walleye pollock, Pacific hake, Pacific cod,
and Pacific tomcod) were listed as being collected from the Hylebos
Waterway does not mean that these fish species did not Inhabit that
location from time to time. Because little or no sport fishing had been
reported 1n the Hylebos Waterway (Personal Comminlcation, Don Novlello,
Tacoma-Pierce County Health Department), no concerted effort wf" made to
collect codfish from that station. The analysis of samples collected
from more popular fishing sites was considered to be more Important.
The selection of the species collected from the Discovery Bay reference
area was primarily dictated by those species caught at the Commencement
Bay stations. As a result, the 11st of species shown as being collected
from Discovery Bay 1s not Indicative of tne relative abundance of these
species 1n the commercial trawl catches from that area.
-------
Condition of Samples
General Condition
The external condition of the crabs appeared to be good with only one
Dungeness crab missing a leg and another having a "scar" on Its
carapace. Although no tumor-like growths were seen on any of the
fish samples, approximately 131 of all the fish collected were
observed to have some type of external abnormal condition. The
abnormalities appeared to have been caused by such things as disease,
predatlon, parasitism, abrasion, or hook and line fishing. The
abnormal conditions recorded are listed 1n Table 0-1 and summarized
below.
The erosion or loss of portions of various fins was observed 1n an
English sole and a whitespotted greenllng from Hylebos Waterway, 1n
an Enallsh sole and a rock sole from the Point Defiance Dock, and in
a walleye pollock from city Waterway. Hemorrhaglng along the dorsal,
anal, and caudal fins was seen 1n a starry flounder from City
Waterway, while "raw looking" p1nk/red splotches were observed on the
blind side of a flathead sole from the sane area. An open tear was
noted 1n the flesh along the caudal peduncle of a walleye pollock
from City Waterway, with a bleeding wound observed on the caudal
peduncle of a rock sole from the Point Defiance Dock. In addition, a
buffalo sculpln from Old Town Dock was missing a portion of Its left
maxillary, and a starry flounder from Discovery Bay had small lesions
on Its blind s1d« that appeared to be associated with the presence of
crustacean parasites.
The highest incidences of abnormality were In fish from City Waterway
(201), Point Defiance Dock (19%), and Hylebos Waterway (18%). Only
6% of the fish from Old Town Dock ana the Discovery Bay reference
area were observed to have external abnormalities.
Abnormalities 1n the fish from the Commencement Bay stations were
most prevalent 1n flatfish (601), with the remainder of the
abnormalities (401) being observed 1n walleye pollock, whltespotted
greenllng, and buffalo sculpln.
Inspection of the fish 11 vers did not result 1n the oetectlon of any
grossly visible lesions or abnormal growths. The most striking
feature of the 11vers examined, however, was the wide variation 1n
their coloration from station to station. For example, the livers of
English sole collected from the Hylebos Waterway ranged 1n color from
yellowish/brown to yellowish/beige with pink or red/brown splotches,
while the livers of English sole taken from Discovery Bay ranged from
pink/beige to gray/brown w'th gray/white splotches.
Although the significance of the color differences was not known,
they may have been linked to such factors as size, age, sex, diet, or
physiological state. It 1s also possible that 1n some cases they
were associated with a degenerative condition, or the presence of
microscopic lesions, as reported by Mallns and his associates (6).
-------
Parasites
As expected, a variety of fish parasites and other associated
organisms were observed during the gross visual examination of a
number of fish collected for analysis. Other than noting the
presence of some of the more noticeable organisms (Table 0-1), no
detailed cause »nd effect studies were undertaken.
One of the most prominent fish parasites recorded was the bloodworm,
Ph1lometra. These parasitic nematodes may have been present in oUier
species and 1n other tissues, however, they were observed under tne
skin, cocmonly along the base of the fins, 1n only English sole and
rock sole (Table 3).
Table 3. Distribution of the Bloodworm, Philometra, in English Sole
and Rock Sole from the Commencement Bay Area and the
Discovery Bay Reference Station
Incidence of Infestation
Station Species (F1sh Parasitized/Fisn collected)
Hylebos Waterway
English Sole
(0/5)
City Waterway
English Sole
(1/5)
Old Town Dock
English Sole
(3/3)
Rock Sole
(1/2)
Point Defiance
English Sole
(2/3)
Dock
Rock Sole
(2/3)
Discovery Bay
English Sole
(0/5)
The Incidence of Philometra 1n English sole ranged from 0/5 1n the
Hylebos Waterway and Discovery Bay, to 3/3 at Old Town Dock.
Although the wide variation 1n the Incidence of Philometra in
flatfishes from the Comnencement Bay area could not oe explained, 1t
was generally consistent with the variation observed by Aralsh (2) In
English sole from other Puget Sound waters. In addition, the overall
frequency of occurrence of Philometra In English sole from all the
Commencement Bay stations (381) was only slightly higher than that
(321) reported by Weltcamp and Schadt (13) in English sole caught
during tha winter from the same general area.
-23-
-------
Size and Age of Samples
The estimated ages of the fish collected ranged from 1 year to 11
years, with most of the fish being from 3 years to 6 years of age.
The average lengths and estimated ages of the most frequently
collected fish species (English sole, walleye pollock, and Pacific
hake) are presented 1n Table 4. For more detailed Information on the
length, age, total weight, fillet weight, liver weight, and sex of
these and other fish collected refer to Table D-l.
The average length of the English sole collected ranged from 246 nan
(Old Town Dock) to 289 mm (City Waterway). The average estimated
ages of English sole collected from these same stations ranged from 3
years at Old Town Dock to 5 years at City Waterway. Walleye pollock
ranged In length from an average of 333 mm (Old Town Dock) to an
average of 409 mm (City Waterway); the average estimated age of these
fish also ranged from 3 years to 5 years, respectively. The average
length of Pacific hake collected from Old Town Dock was 435 mm, with
specimens collected from City Waterway averaging 448 mm 1n length.
Regardless of the species listed In the table, the smallest (and
youngest) fishes were collected from Old Town Oock, with the largest
(and oldest) specimens belna taken from City Waterway. Considering
the small sample sizes Involved (3-5 samples), 1t was not possible to
explain this size and age distribution. It Is Important to note that
although most of the fish species collected were not characterized by
large differences 1n size or age, the differences that did exist
could have accounted for at least some of the variation observed 1n
the result of the chemical analyses.
No effort was made to estimate the ages of the crabs collected. The
Dunaeness crabs ranged 1n carapace width (excluding spines) from 118
mm (Hylebos Waterway, female) to 186 ran (Discovery Bay, male).
-24-
-------
Table 4. Mean Lengths and Estimated Ages of Selected Fishes Collected from the
O—"°nce«ent Bay Area and The Discovery Bay Reference Station.
Stations
Species
Hylebos City Old Town Point Defiance Discovery
Waterway Waterway Dock Boa thou se Dock Bay
(Mean Length,ura/Hean Estimated Aqe.ayrs)
English
Sole
276/3.& 289/5.4 246/3.0
263/4.7
285/5
Walleye
Pollock
409/4.8
33i/3.0
335/4.2
391/4.2
Pacific
Hake
448/-
435/-
a
b
c
Ages estimated from otoliths.
Hean estimated age based on only four of the five specimens collected.
No specimens collected.
-------
REFERENCES
1. American Chemical Society's Committee on Environmental Improvement,
guidelines for Data Aqu1s1t1on and Data Quality Evaluation In
Environmental Chemistry, Analytical Chemistry 52,2242-9 1980.
2. Amish, R.A. The Occurrence of the Bloodworm Phllometra americana
(Nematode: Dracunculoldea) 1n Two Common Flatfishes of Central Puget
Sound. M.S. Thesis. University of Washington. 41 pp., 1976.
3. 8'iazevlch, J. Methods for Analysis of Priority Pollutants 1n
Sediments and F1sh Tissues, PPA, Region 10 Laboratory, Manchester,
Washington. 1980.
4. Compliance Policy Guides Manual. U.S. Food and Drug Administration.
Washington. D.C. 1980.
5. Hart, J.L. Pacific Fishes of Canada. Bulletin 180. F1sh. Res. Bd.
Canada. Ottawa, Canada. 1973.
6. Manns, D.C., B.B. McCain, D.W. Brown, A.K. Sparks, and H.O.
Hodglns. Chemical Contaminants and Biological Abnormalities in
Central and Southern Puget Sound. NOAA Technical Memorandum OMPA-2,
U.S. Department of Commerce, Boulder, Colorado. 295 pp., 1980.
7. Mallns, D.C., B.B. McCain, D.W. Brown, A.K. Sparks, and H.O. Hodglns,
and Sin-Lam Chan. Chemical Contaminants and Abnormalities 1n F1sh
and Invertebrates from Puget Sound. NOAA Technical Memorandum
OMPA-19, U.S. Department of Commerce, Boulder, Colorado. 168 pp.,
1982.
8. Methods for Chemical Analysis of Water and Wastes, U.S. Environmental
Protection Agency, EMSL Cincinnati, Ohio. EPA - 600/4 - 79 - 020,
March 1979.
9. Nestmann, E.R., E.6. Lee, T.I. Matula, 6.R. Douglas, and J.C.
Mueller. Mutagenicity of Constituents Identified 1n Pulp and Paper
Mill Effluents Using the Salmonel1a/Mammal 1 an - Microsome Assay.
Mutation Research 79*203-212, 1980.
10. Novlello, D.T. and S.N. Rogers, CooMencement Bay Seafood Consumption
Study. Draft Preliminary Report. Tacoma-Plerce County Health
Department. Tacoma, Washington. 1981.
11. Riley, R.6., E.A. Crecellus, D.C. Mann, K.H. Abel, B.L. Thomas, and
R.M. Bean. Quantitation of Pollutants 1n Suspended Matter and Water
from Puget Sound. NOAA Technical Memorandum ERL MESA - 49. U.S.
Department of Commerce, Marine Ecosystems Analysis Program. Boulder,
Colorado. 99 pp.t 1980.
12. U.S. Federal Code of Regulations. Title 21, Part 109, Section
109.30. Tolerances for Polychlorlnated B1phenyls (PCB's). 1981.
1',. weitcamp, O.E. and T.H. Schadt. Fish. In: Commencement Bay Studies,
Volume III, F1sh and Wetlands. Report to U.S. Army Corps of
Engineers, Seattle District, by Dames and Moore. Seattle,
Washington. 1-75. 1981.
-26*
-------
ACKNOWLEDGEMENTS
This study represents the cooperative efforts of a number of Individuals
and governmental agencies. Although those who made major contributions
are acknowledged below, the assistance of everyone Involved was sincerely
appreciated.
Doug Pierce. Steve Mfrek, and Don Novlello, Tacoma-Plerce County Health
Department, made many helpful suggestions regarding the location of
stations and the collection of samples. The information and r.,v1ce on
sampling techniques and the distribution of Puget Sound fishes offered by
Dr. Bruce Miller, University of Washington, School of Fisheries, were
also of great value. Greg Bargroann and his staff, State of Washington,
Department of Fisheries, coordinated the collection of samples from the
Discovery Bay reference area, with samples being provided by the crews of
the commercial bottom trawlers "Cape Spencer", "Columbia", and
"Heather." Cleanup of selected tissue samples by gel permeation
chromatography was kindly provided by Dr. David Mitchell, Municipality of
Metropolitan Seattle (METRO) Laboratory. Julie Lyons and Karma Black,
National Marine Fisheries Service, Northwest and Alaska Fisheries Center,
patiently shared their expertise in the use of otoliths to estimate the
age of fishes.
Also appreciated was the support of the following EPA Region 10
personnel; Dr. Gary O'Keal. Director, Environmental Services 01v1s1on,
Dr. Charles Morgan, Statistician, Dr. Michael Watson, Toxicologlst, Ray
Peterson, Environmental Engineer, Eric Meyerson, Visual Information
Specialist, Barbara Haynes, Secretary, and Allyson Turner, Clerk-Typist.
27-
-------
APPENDIX A.
Comparison of Concentrations of PCB's and Pesticides Among Fish Spades
and Sampling Sites
NOTE: "u" denotes concentration 1s less than detection limit
* denotes quantifiable concentrations have been averaged
together with "u" concentrations
For more Information concerning detection limits see Appendix F
page 115.
PT'>
-------
rtgura A-l. Av«r»ga of Total KB and Paatidda Concentration
mm) Bottaa dshaa CoUactad frcm rvux Cmuni—nt
J. o 679 Bay Sitaa and a Rafaranca Station ae DUoovaty Bay
BOTTOM FISHES
Spaclat:
English Sola
Rock Sola
St;rry floundar
C-0 Sola
Fltttead Sola
Ptitlcldai and PCB'i Includad:
DOT
DOE
000
HCB
T* 0.235
T- 0.181
?¦ 0.123
.050
T« 0.016
HyUbot
uatamay
Pt. OaHanca
Oock
City
WaunMy
"oTdrotUT
Oock
Oltcovary
Say
-28-
-------
.650
3C
Figun A-2. Average of "notal PCB ani Pesticide
_ Concantration among Mixed Fishes Collected
0.692 from Hir»e Oanrancerant Bay Sitae and! a
Reference Station at Discovery Bay
MIXED FISHES
8«orss«nt»d SjaclM:
Pacific SUghcrn Sculpfn
Buffalo Seulpln
Whltaspotted Srwnling
Rockflsh sp.
B»it1ctde» and PCB't Included:
PCB
OCT
DOE
000
HCB
X' 0.07J
r« n.nso
No Stnola
Hytrto* Waterway Point DafUnca City Waterway Old Town Pock 0Uc5v«ry
Oock *•»
-29-
-------
.450
.400
I
* .350
Jt
91
S -wo
S
s .250
&
.200
3
a.
2 .150
.100
.050
Figure A-J. Average of Total PCB and Pesticide Concentration
among Off-Bottom Fishee Collected frort Three
Ccrrancepervt Bay Sites ard a Reference Station at
Qiacovery Bay
Off-BOTTOW HSHtS
Represented Soecies:
Pacific Ha lit
Pacific lomeoil
Mil eye Pollock
P»clfle Cod
Pesticides and PCBs Included:
PCB
00T
DOE
000
nee
7' 0.1)96
X- 0.077
n.fififi
Ho S-wple
ttat«rwy
Oock
Waterway
Oock
fldV
-30-
-------
Fiijun A-4. Average of Total PCB and Pesticide concentration
in Enqlimh Sols Muscle Tissue Collected frcm Four
Ccrnnencsnsnt Bay Sites and a Reference Station at
Discovery Bay
ENGLISH SOLE
ZVC .55 PCB
H/
.11 HC8
fgitlcldes and PCB'i Included:
PC8
DOT
DOE
000
HC8
.19 PCB
*.<108 XO
.005 ODE
Mlflfl OPT
.008 000
.oos roe
am dpt
.12 PCB
.33 PCB
<.003 000
.004 00E
nn? opt
4.013 PCB
.003 00E
Nylcboi Uittrway CI ty Materwy Old Tnwn Oock
Point OeManc*
(Vck
Otjcoverr
Hay
.31-
-------
to Samol#
rigur* A-5. Awaraga of total PCB and Pe*ticid« Concentration
in Flathaad Sola Muacla Tissue Collected from One
Ccnrnncmnt Bay Sita and a Referance Station at
Diacovary Bay
FLATHEAD SOLE
Pa«t1c1de* «nd PCB's Included:.
PCB
DDT
DOE
COO
HCB
,033 PCB
.010,, °CB
Ho Samp It
No Sample
Hyietot Waterway City Waterway
Old Town Oock Point Oaflanee
Dock
Olscovery
Sly
32-
-------
.450
Flgur* K-i. Avacag* of total PCS anil Paaticide Concentration
in tock Sol* Muacl* Tlscu* Coilactod frcn TVo
Bay Sitaa
ROCK SOLE
Ptttlcldts and PCB's Included:
PCB
DOT
®E
000
HCB
.026 OOE
«.00« OOT
ammm
.006 OOE
.002 DOT
»
.11 PCB
.12 PCB
No Swtpla No Sanol*
Mo $Amo)»
Hylabos Utttrway CUy U«umay Old Town Doc* Point P#f1«nct Ottcowy
Dock B«y
33-
-------
.450
Figur* K-7. Average of Total PCB and Paaticida Concentration
In Starry riourdar Muacle Tiaaue Collected (ran
One Oamnoaat Bay Site and a Rateranea Station
at Diacowry Bay
STARRY FL0UN0ER
Pesticides and PCB's Included:
PCB
DOT
DOE
000
HCB
No SmwU
.003 000
.004 DOE
.002 not
.18 PCB
.005 nnt
.m? pcb
Ho 5a*nle
in Sample
Hyltbos WattnMy Cfty W«termy Old Town Oock
Point OtfUnet
Oock
01scovery
lUy
-34-
-------
450
400 riquc* A-B. Aimrag* of "total PCS and Paaticida Concentration
in Buffalo Sculpin Muaclo Tittua Coilaetad txaa
350
Two Ooraanemnt Bay Sitaa
BUFFALO SCULPIN
Pntlcldw md PCB's Indudtd
PCS
DOT
OOE
300 000
HCB
250
200
ISO
100
050
to Sanolt
No Stnplt
002 OOE
032 PCS
nn »rn Wo Sanolt
Hylrtoc Watcntiy City MtMnMy
Old Town Dock Point fafMnct
Dock
01 lCWfry
BiV
-35-
-------
.450
M.<,ura A-9. Avaraga of Ibtal PCB and Pauticida Concentration
in Pacific Staqhom Sculpln H»acl« Tisaua Cullactad
from Thraa Ccmnanomnt Bay Sitas and a ftsfmronce
Station at Diacovary Bay
PACIFIC STAGHORN SCULPIN
Pftt1c1d«» «nd PCB'i Includad:
PCB
00T
DOE
0C0
HCB
.031 HC8
.002 000
.002 PCE
.003 DOT
.26 PCB
'In Smpl«
.one not
.nt rrn
.049 PCB
.no? prr
oi. rrn
HylabOS Mtinty City Mttrway Old Towt Dock
Point DefUnct
Dock
OUcavtry
Bay
.36-
-------
.450
ttijura ArlO. Awrag* of Ttotal PCS and Paaticid* Concantration
In tacifc cod ftiacla Tisaua Colldctad from Two
CcMMnomnt Bay Sitat
PACIFIC COO
*> Srft
PtltlcldM ind PCB't Includad:
PCS
DOT
DOE
DX
HCB
.002 rot
.006 DDT
.017 PCB
.002 00E
.004 DOT
.050 PCB
»tf>
Hyltbo* bMttrwty City Wit«rw«y
Old Town Dock Point Oftinct DUeovvy
Oock K«y
-37-
-------
450
400
350
300
250
200
150
100
050
Figure A-il. Avaraga at Tbtal fCB and Pasticida Concantration
in Pacific Haka Muscla Tissue Oollactad fro* Tuo
Oamnomnt Bay Sitas.
PACIFIC HAKE
No SmipU
Putlcldis «nd PCS'* Included:
PCS
OOT
00E
EDO
HC8
002 OOE
002 OOT
.044 PCS
<.002 000
.00* OOE
.004 DOT
.093 PCI
_NoSl«
Mo S«i«olt
Hyltbot W*t*rv«y City Uatsr my Old Town Ooek
Point Defl«nc>
Oock
Dlicovtry
-38-
-------
450
400
350
300
SO
200
ISO
100
oso
0
rigura *-12. Avacaga of Total PCS and Peaticida Concentration
in Pacific Tanccd Muacla Tisaua Oollacted from
Ona Ooimarcanant Bay Sita and t Rafaranca Station
at Diaoawary Bay
PACIFIC TOMCOO
Paftlcidas «nd PCB'l Included:
PCS
OOT
DOE
no
HCB
f .002
Uf
8
.030
PCB
¦m.W one
Kn Samnla
flo S.mu'Io
1 1.'Mo i" n
Witarwiy City M«t»nrty Old Town noe* Point OefUncc Mscnvory
Onck Bay
-39-
-------
.450
Flgun Ar-13.
NO Swpl*
Mungi at total ICS and Pwticlda Concantnticn
in NUlay* Bollock Muaela Tiaaua Collaeead from
Dm aomnomnt Bay Sitaa and a Hafatanoa
Station at Oiaoovary B«y
WALLEYE POLLOCK
Ptltlcldts and PCB't Included:
PCS
00T
ooe
ooo
hcb
.006 OOE
>.oo8 nor
.17 PCS
mm.
.003 ooe
'.002 DOT
.024 PC0
.058 PCS
.009 OOE
(006 00T
<072 PC8
Hylabot Watamay Ctty Uattmay
Old Town Ooek Point Oaftinea
Dock
OMcovary
Uy
-40-
-------
Flgura A-14.
Avvraga of Tbtal PC3 and Ptatieida Concentration
in CXinganasi Crab tJuacla Tisaua CoUactad {ran
Two Ccamannmnt Bay Sifcaa ani a Rafarariea Station
at Oiaoowy Bay
DUNGENESS CRAB
Ptstlcldti and PC8'» tncludtd:
PCI
ODT
OOE
000
HCB
.018 HCB
BBS
.002 OOE
.0S2 PCI
.004 ooe
.002 00T
.068 PCD
JiSJiflSClt.
^sa.tvis o?E
I 1.01
"5
Hylabo* Wattrwiy City Mt«rw«y
Md Toon Dock fotnt
nock
Discovary
(lay
41-
-------
Table A-l-
Average PC# ind httcide Concentrations Found in Fi:*> Muscle Tissue Siapltil in the Coaaenceoent Ity Area and Discovery Bijr ug/g net weight
PCB OOT 00€ OOP HCB
- *£ t'-ufi
Species
(«)
Heat)
Range
Nun
lunge
Mean
Range
Hean
Range
Mean
Range
HylcUu
Uh He spotted
Greenling
1
0.86
0.45-1.12
0.02S
).014-0.037
0.006
0.002-0.01
0.005
0.002-0.01
0.059
).014-0.096
UteiMy
English Sole
S
0.S5
0.13-1.03
:0.006 (4/5]
D.Olti-O.OlS
0.005
0.002-0.007
=0.008(4/5)
o.ooiu-aoi:
0.11
1.056-0.IS
Pacific Staghora
Sculpin
2
0.26
0.*7-0.34
0.003
>002-0.004
0.002
-
0.002
-
0.031
).028-0.034
.
roint Defiance
English Sole
3
0.33
0.10-0.64
e 0.002(1/3)
o.ooiu-aoo5
0.004
0.003-0.005
<0.003(2/1)
O.OOlu-G.OM
O.OOlu
-
Dock
Rock Sole
2
0.1?
0.06-0.19
= 0.004(1/;)
iaiu-o.006
0.026
0.003-0.049
SI.001(1/2)
o.ooiu-o.oo:
O.OOlu
-
C-0 Sole
1
0.07
-
O.OOlu
-
0.001
-
O.OOlu
-
O.OOlu
-
Walleye Pollock
S
Q.0SP
0.017-0.13
D.005(2/5)
o.ooiu-aas
0 004
0.001-0.011
-4.(41(1/5)
3.0010-0.001
O.OOlu
-
Pacific Stai wrr.
Sculpt*
3
0.049
0.045-0.052
a.002(2/2)
o.ooiu-aoos
0.007
0.004-0.011
=0.002(2/3!
).001u-0.00-
O.OOlu
-
Buffalo Sulfin
1
0.011
-
O.OOlu
-
O.OOlu
-
O.OOlu
-
O.OOlu
-
lirni rrpri'M-nu tin* suaU-r of wiaplc* ulik driertable valiwi over tlM nnber of KM|»lrK analysed
-------
Table A-l. coot.
Average PC# and Pesticide CoMMtrttlnis found in Fish Huscle Tissue Sailed in the Coaaenceaent Bay Are* and Discovery Bay ug/g wet weight
pcb dot oot ceo hc?_
itilicr
Species
<«»
Mean
Range
Mean
Range
Hear.
Range
Mean
Range
fiean
Range
City
English Sole
s
0.19
0.054-0.36
0.004
0.002-0.005
0.005
0.004-0.006
0.008
0.002-0.016
O.OOlu
-
Waterway
Starry Flounder
1
0.18
-
0.002
-
0.004
-•
0.003
-
O.OOlu
-
Walleye Pollock
s
0.17
0.017-0.53
D.008(4/5)
).001u-0.026
0.006
3.002-0.015
•=0.001(2/5)
o.ooiu-o.oa
O.OOlu
-
Pacific Hake
5
0.044
0.02-0.073
0.002
1.002-0.003
0.002
}.001-0.003
-0.001(2/5)
0.00^-0.002
O.OOlu
-
Pacific Cod
2
0.037
0.036-0.038
0.006
D.001-0.011
0.002
).001-0.002
O.OOlu
-
O.OOlu
-
Flathead Sole
1
0.033
-
O.OOlu
-
0.001
-
O.OOlu
-
O.OOlu
-
Pacific Toacod
2
0.03
0.020-0.032
O.OOlu
-
0.002
0.002-0.003
O.OOlu
-
O.OOlu
-
Old Iowa
English Sole
3
0.12
0.091-0.16
0.001
0.001-0.002
0.003
0.002-0.003
=0.001(1/3)
o.ooiu-o.oo;
O.OOlu
-
Dock
Rock Sole
2
0.11
0.096-0.12
0.002
0.001-0.0u4
0.006
-0.001(1/2)
O.OOlu-O.OO
O.OOlu
-
Pacific Staghorn
Sculpt*
1
0.10
-
0.001
-
0.008
-
O.OOlu
-
O.OOlu
-
Pacific Hake
S
0.093
0.05-0.14
0.004
0.001-0.006
0.004
0.002-0.008
;0.002(3/5)
B.OOlu-O.OO'
O.OOlu
-
tockfish
1
0.CS9
-
0.002
-
0.007
-
O.OOlu
-
O.OOlu
-
Pacific Cod
1
O.OS
-
0.004
-
0.002
-
O.OOlu
-
O.OOlu
-
Buffalo Sculpin
1
0.032
-
0.001
' -
0.012
-
O.OOlu
-
O.OOlu
-
Walleye Pollock
s
0.024
0.017-0.032
<0.002(3/S)
o.ooiu-o.oo;
0.003
0.002-0.003
O.OOlu
-
O.OOlu
-
FncdM rep resent* the auaber of aaaplea ulth toKUble values over tk> nunbet of uiflu wtljui
-------
Table A-l. cent.
Avenge PCB and Pesticide Concentrations Fotn«J in Fish Huscle Tissue Stapled in the Coaaenceoent Bay Area and Discovery Bay ug/g wet weight
PCB DOT DOT 000 "r.B
itizion
Species
(N)
Hean
Ranje
Hean
Range
Mean
Range
Hean
Range
Hean
Range
Discovery Bay
Ualleye Pollock
S
=0.072(2/5)
O.Olu-O.27
-0.006(2/5)
).001u-0.024
0.009
0.001-0.035
0.001(1/5)
).ooiu-o.oo;
O.OOlu
-
English Sole
5
=0.013(2/5)
o.oiu-o.oi;
=0.001(1/5)
).001u-0.002
0.003
0.002-0.003
O.OOlu
-
O.OOlu
-
Starry Flounder
1
0.012
-
O.OOlu
-
0.005
-
O.OOlu
-
O.OOlu
-
Pacific Sughora
Sculpin
S
0.010,1
-
O.OOlu
-
0.002
0.002-0.004
O.OOlu
- ¦
O.OOlu
-
Pacific Toacod
1
O.OlOu
-
O.OOlu
0.002
-
O.OOlu
-
O.OOlu
-
Flathead Sole
1
O.OlOu
-
O.OOlu
-
0.001
-
O.OOlu
-
O.OOlu
-
Fiaclloa represents tbt nuahei ot Maples with dtlectiblc values over the nuaber of uaplti analyzed
-------
Table A-2.
KB ind Pesticide Concentrations*Found In Fish Muscle Tissue Stapled it the Hyletoos totenaj
lib. Ho.
Species
PCB 1254
Average
DOT
Average
00E
Average
ooo
Average
HCB
Average
36051
English Sole
0.88
0.007
0.006
G.010
0.14
36052
English Sole
0.47
0.007
0.005
0.009
0.12
360S3
English Sole
0.13
0.S5
O.OOlu
¦=0.000
0.002
0.006
O.OOlu
<0.006
0.056
0.11
360S4
English Sole
0.25
o.ooe
0.003
0.007
0.069
J60SS
English Sole
1.03
0.01S
0.007
0.013
0.15
•
444)00
Uhi tf spotted Greenling
1.12
0.037
0.010
0.010
0.096
44001
Uhitespotted Greenling
0.4S
0.86
0.014
0.02S
O.OOS
0.006
0.003
O.OOS
0.066
0.059
44002
Uhitespotted Creent. )
1.02
0.02S
0.002
0.002
0.014
*4003
Pacific Staghorn Sculpir
0.34
0.002
0.002
0.002
0.028
44004
Pacific Staghorn Sculpti
0.17
0.26
0.004
0.003
0.002
0.002
0.002
0.002
0.034
0.031
44005
Pacific Staghorn Sculpin
-
-
-
-
-
-
-
-
-
-
* Concentrations Expre
sed in i
1!% »et
Height
•
-------
Table A-2. cont.
KB tad Pesticide Concentrations'Found in fish Muscle Tissue Stapled it the City waterway .
Species
Pacific Toacoo
PCa 1254
Averts•
DOT
Average
00E
Average
000
Average
HCB
Pacific Toacod
0.032
0.010
O.OOlu
O.OOlu
0.002
0.002
O.OOlu
O.OOlu
O.OOlu
Pacific Toacod
0.028
O.OOlu
0.003
O.OOlu
O.OOlu
Pacific Cod
0.038
0.037
0.001
0.006
0.001
0.U02
O.OOlu
O.OOlu
O.OOlu
Pacific Cod
0.036
0.011
0.002
O.OOlu
O.OOlu
Starry Flounder
0.18
0.1S
0.002
0.002
0.004
0.004
0.003
0.003
O.OOlu
Flatliead Sole
0.033
0.033
O.OOlu
O.OOlu
0.001
0.001
O.OOlu
O.OOlu
O.OOlu
English Sole
0.054
0.005
0.005
0.006
O.OOlu
English Sole
0.16
0.002
0.004
0.002
O.OOlu
English Sole
0.23
0.19
0.002
0.004
0.0C6
0.005
0.013
0.008
O.OOlu
English Sole
0.15
0.004
0.006
0.002
English Sole
0.36
0.005
0.006
0.016
O.OOlu
| O.OOlu
Ualleye Pollock
0.53
0.004
0.007
0.001
O.OOlu
Ualleye Pollock
0.017
0.002
0.003
O.OOlu
O.OOlu
Ualleye Pollock
0.20
0.17
0.026
cO.OOB
0.01S
0.006
0.001
: 0.001
O.OOlu
Ualleye Pollock
0.036
O.OOlu
0.002
O.OOlu
O.OOlu
Ualleye Pollock
0.066
0.009
0.005
O.OOlu
O.OOlu
Pacific Hake
0.020
0.002
0.001
O.OOlu
O.OOlu
Pacific Hake
0.073
0.003
0.003
0.002
O.OOlu
Pacific Hake
0.038
0.044
0.002
0.002
0.001
0.002
O.OOlu
0.001
O.OOlu
Pacific Hake
0.036
0.002
0.002
O.OOlu
O.OOlu
Pacific Hake
0.053
0.003
0.002
0.001
O.OOlu
Concentrations Expressed In uj/q Ue
Height
-------
Tab)* A-2. coot.
K» i*l NitlctM CMceatrattoas* Foua* la Fish Muscle Tissue SjMpled at Utt 014 Tom Dock
Lab. Do.
SfCCiM
PCS 12S4
Average
001
JWera9*
DOC
Averag<
000
Average
HO
to*rage
20001
tockflth
O.OS9
0.059
0.002
0.002
O.Off
0.007
O.OOlu
O.OOlu
O.OOlu
O.OOlu
20002
Pacific SUghora Sculpt
0.10
0.10
0.001
0.001
0.006
0.008
O.OOlu
O.OOlu
O.OOlu
O.OOlu
noo;
kiihb Sculpt*
0.032
0.032
0.001
0.001
0.002
0.002
O.OOlu
O.OOlu
O.OOlv
O.OOlu
29006
Mock Sol*
0.096
0.11
0.001
0.002
0.006
0.006
0.001
<11.001
O.OOlu
O.OOlu
4tt0f
flock Sol*
0.12
0.004
0.006
O.OOlu
O.OOlu
29000
English Sal*
0.16
0.12
0.002
0.001
0.002
0.003
O.OOlu
<0.001
O.OOlu
O.OOlu
29009
English Sol*
0.091
0.001
0.003
O.OOlu
O.OOlu
29040
English Sol*
0.11
0.001
0.003
0.002
O.OOlu
4907$
Alley* Pollock
0.033
0.002
0.003
O.OOlu
O.OOlu
49076
Uilley* Pollock
0.031
0.002
0.003
O.OOlu
O.OOlu
49017
Walleye Pollock
0.017
0.024
O.OOlu
« 0.002
0.002
0.003
O.OOlu
O.OOlu
O.OOlu
O.OOlu
49070
Malicy* Pollock
0.019
0.001a
0.003
O.OOlu
O.OOlu
49079
sialley* Pollock
0.019
0.002
0.002
O.OOlu
O.OOlu
49000
Pacific Co4
O.OSO
O.OSO
0.004
0.004
0.002
0.002
O.OOlu
O.OOlu
O.OOlu
O.OOlu
49081
Pacific Make
0.0S6
0.003
0.003
0.001
O.OOlu
490S2
Pacific Hafc*
O.OSO
0.001
0.002
O.OOlu
O.OOlu
49081
Pacific Hat*
0.14
0.093
0.006
0.004
0.008
0.004
0.004
«= 0.002
O.OOlu
O.OOlu
49084
Pacific N*k*
0.12
0.004
0.002
O.OOlu
O.OOlu
4908$
Pacific Hak*
0.10
0.004
0.104
0.003
O.OOlu
* CMtMtratiMt Eipm
td i* u
/9 Met
ieight
-------
Tab)* *-2. coot.
PCt and Pesticide Concentrations*Found in Fith Muscle Tissue Stapled at the Point Defiance Dock
Lab. Mo.
Species
l>Ct 12S4
Average
DDI
Average
00E
Average
000
Average
HCt
Average
28000
Rock Sola
0.19
0.006
0.049
0.001
O.OOlu
29000
Rock Sola
0.060
O.l.1
O.OOlu
cO.004
0.003
0;026
O.OOlu
-cO.OOl
O.OOlu
O.OOlu
29001
lock Sola
.
_
-
-
-
-
-
-
-
29002
English Sole
0.10 •
O.OOlu
0.003
O.OOlu
O.OOlu
29003
English Sola
0.64
0.33
O.OOlu
=0.002
0.005
0.004
0.001
<0.003
O.OOlu
O.OOlu
38027
English Sola
0.25
0.005
0.005
0.006
O.OOlu
•
29004
C-0 Sole
0.070
0.070
O.OOlu
O.OOlu
0.001
0.001
O.OOlu
O.OOlu
O.OOlu
O.OOlu
38028
Buffalo Sculpt*
0.011
O.OU
O.OOlu
O.OOlu
O.OOlu
O.OOlu
O.OOlu
O.OOlu
O.OOlu
O.OOlu
01000
Ualleye Pollock
0.08S
0.008
0.006
0.001
O.OOlu
01001
Ualleye Pollock
0.031
O.OOlu
0.001
O.OOlu
O.OOlu
01002
Ualleye Pollock
0.027
0.0S8
O.OOlu
•eO.005
0.001
0.004
O.OOlu
c 0.001
O.OOlu
O.OOlu
01003
Ualleye Pollock
0.017
O.OOlu
0.002
O.OOlu
O.OOlu
01005
Ualleye Pollock
0.13
0.015
O.OU
O.OOlu
O.OOlu
01004
Pacific Staghorti Sculpin
0.04S
O.OOlu
0.004
0.001
O.OOlu
01006
Pacific Sughora Sculpin
0.050
0.049
0.001
<0.002
0.011
0.007
O.OOlu
S 0.002
O.OOlu
O.OOlu
01007
Pacific Staghoni Sculpin
0.052
0.005
0.006
0.003
O.OUu
* Concentration Express
d in w
/g Uet
¦ight
-------
Table A-2.cont.
KB tiri Pesticide Concentrations Found in fish Ikiscle Tissue Sm»M at Discovery (ay
lat. ito.
Species
PCS 1254
Average
DOT
Average
OK
Averagi
000
Average
HC8
Average
40000
SUrry Flounder
0.012
0.012
O.OOlu
O.OOlu
0.005
0.005
O.OOlu
O.OOlu
O.OOlu
O.OOlu
46005
Flathead Sole
0.01Ou
O.OlOu
O.OOlu
O.OOlu
0.001
0.001
O.OOlu
O.OOlu
O.OOlu
O.OOlu
40014
Pacific Staghorn Sculpt*
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
48015
Pacific sughora Sculpti
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
48016
Pacific Staghorn Sculpt*
O.OlOu
O.OlOu
O.OOlu
O.OOlu
0.002
0.002
O.OOlu
O.OOlu
O.OOlu
O.OOlu
48017
Pacific Suqhorn Sculpin
O.OlOu
O.OOlu
0.004
O.OOlu
O.OOlu
48018
Pacific Staghorn Sculpin
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
48019
Pacific Toacod
O.OlOu
O.OlOu
O.OOlu
O.OOlu
0.002
0.002
O.OOlu
O.OOlu
O.OOlu
O.OOlu
48000
English Solo
O.OlOu
O.OOlu
0.003
O.OOlu
O.OOlu
48001
English Solo
0.017
0.002
0.002
O.OOlu
O.OOlu
48002
English Sole
0.017
«0.013
O.OOlu
« 0.001
0.003
0.003
O.OOlu
O.OOlu
O.OOlu
O.OOlu
48003
English Solo
O.Olfti
O.OOlu
0.001
J.OOlu
O.OOlu
48004
English Sol*
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
48007
Ito 11eye Pollock
O.OlOu
O.OOlu
0.001
O.OOlu
O.OOlu
Ualleye Pollock
O.OSS
0.006
0.006
0.002
O.OOlu
48010
Halteye Pollock
0.27
:0.072
0.024
« 0.006
0.035
0.009
O.OOlu
«0.00i
O.OOlu
O.OOlu
48012
Ualleye Pollock
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
48013
lb 11eye Pollock
O.OlOu
O.OOlu
0.002
O.OOlu
O.OOlu
* Concentrations Expres
ed in •<
l/g Met
feight
-------
Table A-3.
PCI *4 Pesticide Conce»trettOAS*Found la Crab Uncle ItitM Stapled at the Hylebos Mate ray, CUy Waterway and Discovery Bay
lab. No.
Species
KB 1ZV
Average
DOT
Average
DOE
Average
DOD
Average
HCB
Average
xosa
Pungeness Crab
o.osa
O.OS2
O.OOIh
^0.001
0.001
0.002
0.001
*0.001
0 019
0.018
34059
OwttMts Crab
0.047
0.001
0.002
O.OOlu
0.018
Station:
City Itoterway
lib. No.
Species
PCS 125'
Average
DOT
Average
DOE
Average
000
Average
HCB
Average
360S6
Dunqenesi Crab
0.063
0.068
0.002
0.002
0.005
0.004
O.OOlu
c0.001
O.OOlu
O.OOlu
36057
Oungeness Crab
0.053
0.001
0.004
0.001
O.OOlu
Station:
Oiscovery Bay
UI/. Nb.
Species
PCB 1254
Average
OPT
Average
OOF
Average
DOO
Average
HCB
Average
4800b
Duajenesi Crab
O.OlOu
O.OlOu
O.OOlu
O.OOlu
0.006
0.005
O.OOlu
O.OOlu
O.OOlu
O.OOlu
48011
Owyiieti Crab
O.OlOu
O.OOlu
0.004
O.OOlu
•
O.OOlu
* Concentration Express
id in u<
'9 Met 1
jtght
-------
APPENDIX B.
Comparison of Concentration of totals Among F1sh Species and Sampling Sites
NOTE: "u" denotes concentration 1s lass than detection limit
" denotes quantifiable concentrations have been averaged
together with "uM concentrations
For more Information concerning detection limits see Appendix F
page 115.
-------
I* 27.6
Figure B-l. Avaraga of Total Matal Concentration mranq
Botton Flahaa Collected from Four Cartmancament
30TTQM FISHES Bay Sitae and a Reference Station ae Olsoowy Bay
Represented Species:
English Sole
Rock So It
Starry Flounder
C-0 Sola
n«ttw«d sol*
Hettls Included:
Arsenic
Cadnlun
ChroaHua
Copper
Load
"Harcury
Ntckal
SelenluM
Silver
Zinc
T« 12.3
T» 11.4
T' n.i
City
MatanMy
?• 9.4
04 icovfy
Bay
9t. OefUnce
Dock
HyletOS
Wattrwey
Old Town
Oock
-51-
-------
22.5
Figura B-2. Awraga of 'total total Concentration strong
Hixad Fiahaa Coilactad fran Thraa ccnmnoanant Bay
3-i.taa and a Rafazanc* Station at Diteuvary Bay
20.0
„ 17.5
«
i
i
MIXED FISHES
Rapratantad Sa«ctt».
Pacific St»9horn Sculpln
Buffalo Sculpln
Wi1t«Jpottad GrMnHng
Rockfish
Hatali Includad:
Arsanlc
Cadmium
Chromium
Coppar
Laad
narcury
Nlekal
SaltnluM
St War
Zinc
$ 1S.0
§
tm
§ 12.5
r- 12.8
r- t2.o
Si
£ 10.0
*
T« 10.0
T« io.o
I 7,1
ui » • <#
s.o
2.5
J-
rtn Samgl*
CI. oa^inei
Dock
Hylaboi
Watamay
Old Town
Oock
City
Watarwiy
Otscovary
Say
-52-
-------
22.5
riguc* B-3. Avaraga of total natal Concentration aranq Off-Bottom
Fiahae Collactad fran Thraa Canrancanant Bay Sitaa
and a Rafaranca Station at Diaeouary Bay
OFF-BOTTOM FISHES
Baprasantad Spaclat: Mttals Includad:
T> 8.4
Piclfle Hikt
Ptc1f1c Toncod
Wtlltya Pollock
P»c1f1c Cod
Ar»#nte
Ctdmfw
C froaliai
Coppar
La«d
mrcury
Ntckal
Stltnlua
S11 var
Zinc
T> 3.0
No Swpla
T- 7.8
X« 8.5
Point Oaflinct Hylabol Witarway Old Towi Oock CIty arway QHeovary
Dock »•»
-53-
-------
Flgura B-4. Avaraga of Total Matal Qaicantration tn
English Sola Kacla Tlaaua CoLlactad fzaa
Four Gamnoamant Bay Sitaa and a Hafaranca
Staelcn ae Dla^vwry Bay.
ENGLISH SOLE
W»t<1» Includad:
Arwnlc
CtdnluM
Utd
5.120 In
1.260 N1
.246 P*
276 Cu
.232 Cr
1.940 At
fltrcury
Mekal
Chrmfia Saltnlu*
Cappar $11 vat-
Zinc
5.460 Zr.
.648 N1
.322 Pb
:J!I &
5.060 At
5.933 Zfl
.457 Nt
.577 Pb
.383 Cu
.137 Cr
2.900 At
6.533 Z«
.520 N1
3.930 Pb
.393 Cu
.277 Cr
8.566 Ai
5.200 Zn
.225 11
.464 Pb
.416 Cu
.062 Cr
1.220 Ai
Hylabot Watarwiy City Mtomay Of
.54-
-------
riqur* B-5 Avaraga of Tot*! rtatal Cancantratior in
Flathaad Sol* Muacla Tisaue Coliactad {ran
Ona Camancanant Bay Sita And a Rafaranoa
Station at Diaoovary Bay
FLATHEAD SOLE
Het«U Included:
Arltnlc Mercury
Cichlim Nickel
Chromium Stlerium
Copptr Sllvar
iMd 21nc
No SmoW
4.300 Zn
. soo n<
.350 Pb
.250 Cu
.470 Cr
1.000 *i "0 imp\t
no SmpU
4.000 Zn
.200 N<
.310 Pb
.280 Cu
.070 Cr
5.300 At
H/Wbos W»t«n*y CHy M«t«rv«y Old Town Dock
Point OtfUnc*
0OC*
Otscovary
B»y
-55
-------
rtgur* B-6 Avaraqa of Ttotal Matad Concentration in
Rock Sol* Miacla Tissua Collactad fran
Two Qcmnwcmnt Bay Sitaa
ROCK SOLE
Natilt Included:
9.133 Zn
Aronle Mtrcury
C*
-------
Figura B-7 Avorsgs of total Metal Concentration in
Suirry Floundar Muscle Tii»u» Collactad
(rem One Ccnmancmnt Bay Sit* aid a
Mfannc* Station at Diacovsry Bay
STARRY FLOUNDER
Metals Included:
Arsenic Mercury
Cadmium Nickel
Chrcmiom Seleniun
Copoer SIlv«r
Lead Z1nc
Ho S«np1«
5.300 Zn
.560 N1
.350 Pb
.300 Cu
.270 Cr
2.100 As
Mo Sample
Ho laople
«.7no Zn
.ISO m
.810 Pb
. ISO Cu
. ian rv
. (n'1 **
Hylebot Waterwey CUy Waterway Old Town Bock Point OeMtnee Discovery
Dock My
-57-
-------
22.5
ttgurm B-«. Awa?* of Tbtal Hatal CP"rsr.U»tion in
Buffalo Sculpin Musda Tisaua CoUactad
£roa TVo Ojuin-iMnt Bay sitas
20.0
BUFFALO SCULPIN
17.5
Mttlls Included:
*rs«n1c *«rcury
Cidmlm Nlckil
Chromium Stlanlum
Coppor SI Ivor
1S.0
12.5
10.700 Zn
10.0
7.5
11.000 Zn
5.0
•
1.000 N1
M cS
.190 Cr
2.5
.430 N1
3.600 At
.580 Pb
.430 Cu
No Swli No SwoU
No $M0l«
0
.150 Cr
Hyltbos MUrttiy City UtUntay Old Town Ooek Point Otflinct Olscovtry
Dock Siy
-58-
-------
22.5
Figura 3-9. Avarag* of Total Metal Concentration in
Pacific Staqhom Sculpin Muaela Tijaue
Collactad from Three Canmncsnane Bay Sitae
and a .tetaranca Station ae Diaoovary Say
PACIFIC STAGHORN SCULPIN
Hetalt Included:
Arsenic
Cadmlu*
Chromium
Copper
Laid
Mercury
Nickel
Selenium
Silver
Zinc
¦
10.00 Zn
8.000 Zn
6.600 Zn
•
7.000 Zn
.254 N<
.197 Hi
.366 Pb
1
.167 N1
.410 Pb
.543 Cu
.323 Cr
.733 Al No Simple
.900 N1
¦MM
.043 Hg
___
.450 Cu
.112 Cr
.720 Pb
.527 Pb
.887 Cu
.107 Cr
.713 A»
2.040 A»
.720 Cu
.250 Cr
.310 As
Hyleboi waterway City Waterway Old Town Dock
Point Oaffence
Dock
Oltcovery
Bey
-59
-------
22.5
Fiqura B-10. Auarag* of Tr* J "-tal Concantration in
Paciiic Cod i TiMua Collactad trait
TVo ^r~-' . Bay Sitaa
PACIFIC COD
HtUW Included:
Arjenle
Cadmlim
Narrury
Hek«l
Chromlu* S«)*tt
-------
25 5
Figure B-li. Average of Ttatal Metal Concentration in
Pacific Hake Muscle Tisaua Collactad from
IVo Oaimancanant Bay Sitaa
20.0 PACIFIC HAKE
letalt In<.''id«d:
Artenle Mercury
Cadmium Hlcktl
17.S Chromfiai Selanlun
Coppar Silver
Lead Zinc
15.0
12. 5
10.0
NO S«npW
4.780 In
4.740 Zn
.2P6 N1
.250 N1
.120 HQ
.532 Pb
.350 Pb
.374 Cu
.430 Cu
.120 Cr
.064 Cr
.534 At
.640 At
No Swpl*
Hyltbo« Waterway City Wat»n«iy Old Town Oock
Point Pt'Unca
Oock
Oltcovery
hy
-61-
-------
riguea fr>U. Minqt of Total natal Concentration in
Pacific Ttoood Huacla TUaua GoHaetad
tea CM Cuiuitcirt Bay Sita and a
Station at Oiaoowry Bay
PACIFIC T0MC00
*et»U (ncludad:
Arttnle
C*d«1u«
Uad
Htreury
Nickel
Chr«1ua StUnlia
Coppar Silver
Zinc
12.5
10.0 .
7.1 .
5.000 Zn
Ho SaneIt
5.257 Zn
.113 Hi
.501
.457 Cu
.190 Cr
i ii Ha
JSSL
IjssIl.
•iiSLiJ&BlS-
.130 11
.420 Pb
.400 Cu
.080 Cr
3.400 4*
Hylabo* Uatantay City Watarway 014 Towa Deek
Paint D«fl«nea
oock
Oltcovary
lay
62-
-------
2:.5
20.0
17.5
$ 1S.0
W
*
9
•»*
9
S 12.5
85
s
10.0
*
H-
*
4
figure B-13.
Averaga of Totil Metal Concentration in
Walley® Pollock Muscle Tiasue Collected
from ttiree Ccmnancaiant Bay Sites and a
Reference Station at Diacovary Bay
WALLEYE POLLOCK
Metals Included:
Arsantc
Cadmtui
Mercury
Nickel
Chromtjn Selenium
Copper Silver
Lead
21 nc
7.S
¦
6.060 Zn
5.340 Zn
5.080 Zn
5.160 Zn
S.O
____
.268 N1
.256 Hi
.212 N1
.082 Hg
2.5
.300 N1
.076 Hq
.342 Pb
.606 Cu
.084 Cr
.044 Hq
.606 Pb
.822 Cu
125255
.078 Ho
.352 Pb
.532 Cu
.538 Pb
___
.112 Cr
.072 Cr
.548 Cu
1.880 As
1.740 As
0
No Sample
.094 Cr
.772 As
1.440 As
Hylebot Waterway City Uaterwti
Old Town Dock
Point Oe'lanee
Dock
Olscovery
Bay
-63-
-------
Nguca b-H. Avaraqa of Total Natal Conor Lritian in
Kiii jmw¦ Crab Muacla Tlsaua Coliactad
from two ConMncanant Bay Sitaa and a
Ralararca station at Dlaoowy Bay
nh
"\r
rv~i
49.300 Zn
.235 Ag
*.110 U
.7*5 111
.075 M
.825 ?b
12.700 Cu
.190 CP
z.aso ai
DUNGENE SS CRAB
HtUlt lnc)ud«d'
15.000 Cu
Ananie
C4d*1um
ChronluM
Cod par
L(«d
(loreury
N(ck«l
S«l«n1j»
Sllvor
Zinc
49.700 Zn
52.650 Zn
Mil
.875 H1
.085 Hq
.590 Pb
.315 Cr
5.750 At
No
.195 Ag
,07 St
1.535 N1
.070 Hq
.ICS Pb
4.300 Cu
.0C5 Cr
7.250 At
Sinpli
fl°
Hyltbo* Mattney City Waterway
Old Tqma Ooek Point 0«f1»nc»
Dock
01tcov«ry
by
-64-
-------
Utile 8-1.
Average Met*I ioncentrations Found in Fish Species Stalled in the Comenceaent Bay Area mi' I Reference Station at Discovery 8jy uq/q wet weight
C-0 Sdlt (N( Arsenic Caitoiiai ChrtMiiua Copper lead Mercury Aickel Seleniuu Silver 2inc
Hylebos Waterway
City Waterway
Old Town Dock
Point Defiance Oock (1)
37.8
0.006
0.18
0.44
0.65
0.02
O.SO
0.06
O.OOfcu
10.7
Discovery (ay
chgiish sac
-------
I.ililr B-l.
-------
Table 1-1. coat.
Average Netal Concentrations Found la Fish Species Stapled la the Commencement hy Are* end • Reference Sutton «t O'icartri ley ug/g wet nelgtt
MCIF1C SIACHOU SCUtfW (») Arsenic Ciihlui Oroniun Copper le>< Mercury . Wlcfcel Selenium Stiver 21 wc
Hyltbos tUterwey ())
0.73
0.021
0.32
0.54
0.41
0.02
0.17
0.08u
O.OlOu
7.0
City Utterwey
Old Tom Dock (1)
0.31
0.012
0.2S
0.72
0.72
0.03
0.90
0.12
0.006u
10.0
Point Oefiance Doct (31
0.71
0.004u
0.11
0.89
0.53
0.04
0.20
0.07u
0.007m
8.0
Discovery 8«y (5)
2.0
« 0.004
0.11
0.45
0.37
0.03
0.26
G.07u
O.OlOu
6.&
ROCKflSM (H)
Hyletes UnteriMy
City Meter**y
Old Toun Bock (1)
0.55
0.012
0.28
0.6*
0.58
0.03
0.48
0.14
0.006u
9.6
Point Defience Dock
Oiscovery hy
IMITESPOTIEO GREEM.IN6 (H)
Hytebos U«Urw*y (1)
0.71
0.010
0.49
0.42
0.31
0.17
0.18
C.08u
O.OlOu
8.3
City IbtenMy
Old lown Dock
Point Oefience Dock
Oiscorery b/
-------
Idble B-l. coot.
Average Reul Concentrjtions Found in Fi«h Species Stapled in the CtMenceaent B*y Are* *nd i Reference SUtion it Discovery Bay ug/9 wet weight
PACIFIC COO (N) Arsenic C."%iua Chroaiun Copper te«d Hercury Hicfcel Seleniuw Silver Iinc
Hylebos UttenMjr
City UiitiMjf C(
1.8
0.014
0.12
0.32
0.38
0.04
0.26
0.07u
O.OlOu
S.2
Old Town Dock (1)
3.1
0.004
0.07
0.52
0.42
0.12
0.40
0.07u
O.OlOu
e.o
Point OefMKC Dock
Olscovery by
PACIFIC HAKE (H)
Hylebos IMtenMy
City MterMy (5)
0.53
0.012
0.12
0.37
0.S3
0.07
0.25
0.07u
O.OlOu
4.8
Old lawn Dock (5)
0.64
0.006
0.06
0.43
0.35
0.10
0.29
0.07u
O.OlOu
4.7
Point Oefianc* Dock
Discovery |i|
PACIFIC TONCDO (N)
Myletos Hittnay
City Htttnuy (3)
0.70
0.013
0.19
0.46
O.SO
0.03
0.31
* 0.07
0.009u
5.1
Old Town Dock
Point OefiMce Oock
Oi scovery My (I)
3.4 I 0.006
0.00
0.40
0.42
0.0«
0.13
0.07u
O.OlOu
5.0
-------
Table B-l. cont.
Ave
-------
lable 8-2. Average Hetal Concentrations Identified in Fish Muscle Tissue Sampled in the
Cowenceaent Bay Area and a Reference Station at Discovery Bay, ug/g wet weight
Slat 10'
Species
(N)
Mean
Hean
Mean
Hean
Hean
Hean
Mean
Mean Hean
Hean
Ranqe
Range
Mange
Range
Ranoe
Ranae
Ranae
Ranue
Ranae
fcange
Pi. Defiance
Cmi>
C-0 Sole
1
37.8
0.006
0.18
0.44
0.65
0.02
0.50
0.06
0.006u
10.7
toe I Sole
3
16.2
2.5-34.1
0.007
0.006-.008
0.24
0.20-0.28
0.41
0.35- 0.47
0.48
0.4S .0 55
0.05
0.02-0.05
0.49
0.36-0.60
0.24
0.lfi-C.2Q
0.006u
9.1
8 B-9.8
English Sole
3
8.6
3.2-14.6
0.007
0.004-0.01
0.28
0.20-0.41
0.39
0.27-0.49
3.9
0.67-10.4
0.03
O.O2-0 03
0.52
0.40 0.73
0.13
O.Ofu -0.17
0.006u
006u-.007u
6.5
5.3-7.3
Buffalo Sculpin
1
3.6
0.005u
0.19
0.37
0.40
0.02
0.10
0.06u .
0.007u
10.7
Pacific Slagnorn
Sculpin
3
0.71
0.60-0.8/
0.004u
0.11
0.08-0.12
0.89
0.66-1.2
0.53
0.42-0.67
0.04
0.03-0.07
0.20
0.12-0.25
0.07u
0.007u
8.0
l r,-p ?
Walleye Pollock
S
1.4
0.40-2.2
0.004u
0.11
0.08-0.14
0.82
0.71-0.85
0.61
0.53-0.63
0.04
0.02-0.08
0.26
0 19-0.40
0.07u
0.007u
5.1
4.7-6.0
Hylebos
UUerway
English Sole
5
4.9
3.0-7.3
0.008
.006-.011
0.23
0.11-0.62
0.28
0.25-0.^1
0.25
0.18-0.36
0.03
0.02-0.04
1.3
1.2-1.4
-= a.14
0.08u-0.38
0.07u
5.1
4.8-5.6
Uhitespotted
Green)ing
3
0.71
0.44- 0.89
0.01
0.006-0.019
0.49
0.39-0.65
0.42
0.35- 0."Z_
0.31
0.280.37
0.17
O 07-0.23
0.18
0.17-n.?n
0.08u
O.Olu
8.3
7.7-9.0
Pacific Staghorn
Sculpin
3
0.73
0.51-1.1
0.02
0.017-0.025
0.32
0.30-0.34
0.S4
0.44- 0.65
0.41
0.22-0.70
0.02
0.01-0.04
0.17
0.1-0.23
0.08u
O.Olu
7.0
6.3-7.7
-------
lable B-2 cant. Average Heidi Concentrations Identified in Fish Huscle Tissue Sampled in the
Coanenceaent Bay Area and a Reference Station at Discovery Ray, ug/g wet weight
Station
Species
(N)
Mean
Mean
Mean
Mean
Hean
Hean
Hean
Hean
Hean
Hean
(Unqe
Ranoe
Ranoe
Ranoe
Ranw
Ranoe
Ranoe
Ranae
Ranoe
Panoe
Old Town
Ccck
t'acific Staghorn
Sculpin
1
0.31
0.012
0.25
0.72
0.72
0.03
0.90
0.12
0.006u
10.0
Buffalo Sculpin
1
0.13
0.004
0.15
0.43
0.58
0.02
0.43
0.06u
0.009
11.0
Rock Sole
2
4.6
1-5- 7.8
0.008
9.006-0.009
0.21
0.14-0.28
0.40
0.39- 0.41
0.57
0.33- 0.81
0.02
0.01- 0.03
0.38
0.27-0.50
0.07u
0.0611-0.084
O.OOSu
3.006u-O.OU
6.4
6.0-6.8
Rock fish
1
0.55
0.012
0.28
0.66
0.58
0.03
0.48
0.14
O.OOou
9.8
Pacific Cod
1
3.1
0.004
0.07
0.52
0.42
0.12
0.40
0.67u
O.Olu
6.0
English Sole
3
2.9
2.1-3.4
0.007
1.006-0.008
0.14
0.13-0.15
0.38
0.35-0.40
0.58
0.49-0.69
0.03
0.02-0.04
0.4j
0.44-0 46
C.12
0.39-0.15
0.006.
5.9
5 5-ft 1
Ualleye Pollock
5
1.9
1.1-3.4
0.006
1.004-0.006
0.08
0.07- 0.1?
0.61
0.41-0 HI
0.31
n 11-n *7
0.08
0.04-0.12
0.27
0.17-0.37
0.07u
O.Olu
4.5-1.0
Pacific Hake
5
0.64
O.SO-O.90
0.006
0.004-0.016
0.06
0.05- O.Ofl
0.43
0.33-0.67
0.35
0.31-0 39
0.01
0.07-0.13
0.29
0.17-0.40
0.07u
O.Olu
4.7
4.0-5.7
City
Waterway
English Sole
5
i.i
2.8-8.6
O.OOSu
0.31
0.20-0.64
0.32
O ?«-!> «1
0.32
0.20-0.45
0.04
0.01-0 09
0.65
O 4R-1.7
0.07u
O.Olu
5.5
a 1-7.7
Starry Flounder
1
2.1
0.005u
0.27
0.30
0.35
0.04
0.56
0.30u
O.C07u
5.3
Walleye Pollock
5
0.77
0.43-0.97
0.015
0.013-0.016
0.09
0.07-0.12
0.55
0.44-0.71
0 54
0.41-0.68
0.08
0.06-0.09
C.30
0.15-0.40
0.07u
O.Olu
6.1
S 3-6 7
Pacific Cod
2
1 8
1.8-1.9
0.8i3-0.016
0.8}-S.16
0.?$?S.35
0.$}-8.42
0.04
0.04-0.05
0.26
0.25-0.28
0.07u
O.Olu
5.2
5.0-5 3
Pacific Toacod
3
0.70
0.30-1.3
0.013
0.006-0.019
0.19
0.12-0.27
0.46
0.36-0.54
0.50
0.45-0.55
0.03
0.02-0.05
0.31
0.25-0.41
0.07
0.06-0.07u
0.009u
0.006u-0.01
5.3
5.0-5.5
Flathead Sole
1
1.0
0.005u
0.47
0.25
0.35
0.04
0.50
0.30u
0.007u
4 3
Pacific Hake
S
0.53
0.33-0.90
0.012
0.011-0.013
0.12
0.06-0.23
0.37
0.27-0.53
0.53
0.36-0.78
0.07
0.02-0.13
0.25
0.15-0.30
0.07u
O.Olu
4.8
4.3-5.3
-------
Table 8-2 cont. Average Metal Concentrations Identified in rish Muscle Tissue Sampled in the
Comenceoent Bay Area and a Reference Station at Discovery Bay, ug/g wet weight
Station
Species
(H)
Mean
Mean
Mean
Hean
"•ear.
Hean
Hean
Hear
Hean
Mean
kange
Range
Range
Range
Ranqe
Ranqe
ttanqe
Kanae
Ranee
Range
isco/eiy Bay
flathead Sole
1
5.3
0.004u
0.07
0.28
0.31
0.04
0.20
0.07u
O.OIj
4.0
Pacific Staghom
Sculpin
S
2.0
0.90-3.1
< 0.004
).004u-0.00(
0.11
0.09-0.14
0.45
0.33-0.52
0.37
0.27-0.45
0.03
0.02-0.04
0.26
0.13-0.36
0.07u
C.Olu
6.6
5.0-7.3
English Sole
s
3.2
2.1-4.0
= 0.006
3.004u-0.00t
0.06
0.05-0.07
0.42
0.40-0.44
0.46
0.31-0.61
0.04
0.02-0.05
0.23
0.17-0.33
0.07u
O.OIj
5.2
4.3-7.0
Pacific Toacod
1
3.4
0.006
0.08
0.40
0.42
0.04
0.13
0.07u -
O.Olu
5.0
Walleye Pollock
s
1.7
O.g-4.1
« 0.005
1.004u-0.006
0.07
0.05-0.1?
0.53
0.33-0.93
0.35
0.31-0.42
0.08
0.03-0.11
0.21
0.17-0.26
0.07u
O.Olu
5.2
4.7-6.7
Starry Flounder
1
0.7
0.013
0.48
0.35
0.61
0.05
0.18
0.17
0.007u
4.7
-------
Table B-3.
HeConcentrations Found in Fish Muscle Tissue Sampled at the llylebos Waterway , ug/g wet weight
Ub. No.
Species
Sb
As
Be
Cd
Cr
Cu
Pb
Hg
Ni
Se
*9
T1
Zn
36051
English Sole
,2u
7.0
.OOSu
.008
.62
.31
.36
.03
1.2
. 06u
. 00?u
.04u
5.6
36052
English Sole
.2u
3.3
. 005u
.006
.11
.26
.21
.03
1.2
. Otkj
• 007u
.04u
4.8
36053
English Sole
• 2u
7.3
. OOSu
.008
.15
.30
.27
.04
1.3
.38
.007u
.04u
5.0
16054
English Sole
• 2u
4.1
. 005u
.011
.17
.26
.21
.04
1.4
08u
.007u
.D4u
5.2
36055
English Sole
• 2u
3.0
.OOSu
.006
.11
.25
.18
.02
1.2
.08u
,007u
,04u
5.0
44000
UMtespotteJ Greenling
• 07u
.81
.006u
.006
.43
.35
.28
.23
.20
08u
.01..
.04u
7.7
44001
Uhitespotted Greenling
,07u
.44
,006u
.006
.65
.47
.37
.07
.17
08u
.Olu
.04u
9.0
44002
Uhitespotted Greenling
,07u
.89
.006u
.019
.39
.44
.28
.22
.17
.06u
.Olu
. 04 u
8.3
44003
Pacific Staghorn Sculpin
.07u
.51
. 006u
.025
.34
.54
.70
.04
.10
.08u
01b
,04u
7.0
44004
Pacific Staghorn Sculpin
• 07u
.59
• OOfiu
.020
.30
.44
.22
.02
.17
,08u
.Olu
.04u
6.3
44005
Pacific Staghorn Sculpin
,07u
1.1
,006u
.017
.33
.65
.31
.01
.23
.Otu
.Olu
.04u
7.7
-------
Table 1-1 cont.
Metal Concentrations Found tn Fish Muscle Tissue Stapled at the City Uaterwiy. ug/g Met weight
lab. No.
Species
Sb
As
Be
Cd
Cr
Cu
Pb
H9
Hi
Se
*9
T1
Zn
29005
Pacific loacod
• 07u
.5
.005
.006
.18
.54
.55
.03
.41
.06
006u
• 03u
5.5
46051
Pacific Toacod
.07u
.3
• 005u
.013
.27
.47
.51
.05
.25
• 07u
• Olu
• 04u
5.0
46052
Pacific Toacod
.07u
1.3
. 005u
.019
.12
.36
.45
.02
.28
.07u
.Olu
.04u
5.3
38022
Starry Flounder
¦ 07u
2.1
• OOSu
.00r4l
.27
.30
.35
.04
.56
.3u
.007u
.04u
5.3
46050
Pacific Cod
.07u
1.9
.005u
.016
.16
.35
.33
.05
.28
. 07u
.Olu
• 04u
5.3
46058
Pacific Cod
,07u
1.8
.OOSu
.013
.07
.29
.42
.04
.25
• 07u
.Olu
,04u
5.0
38026
Flathead Sole
•07u
1.0
.OOSu
.OOSu
.47
.25
.35
.04
.50
*. 3u
,007u
¦ 04u
4.3
36050
English Sole
.07u
6.2
.OOSu
.OOSu
.26
.26
.20
.03
1.2
• 07u
• Olu
. 04u
5.3
38021
English Sole
07u
3.2
• 005u
.OOSu
.64
,?9
,15
09
.52
07i,
01„
¦ 04u
7.7
38023
English Sole
.07u
2.8
• OOSu
OOSu
.22
.27
.29
.03
.48
.07u
.Olu
. 04u
5.0
38024
English Sole
,07u
8.6
.OOSu
.OOSu
.20
.28
.32
.06
.54
,07u
.Olu
.04u
5.0
38025
English Sole
• 07 m
4.S
• OOSu
,005u
.24
.27
.35
.01
.50
.07u
.Olu
.04u
4.3
46053
Walleye Pollock
¦ 07 m
.97
.005u
.016
.12
.64
.66
.08
15
07u
.Olu
.04u
M
46054
Walleye Pol'ock
.07u
.43
.OOSu
.016
.12
.71
.48
,w
,35
,07u
.Olu
04i.
6.7
46ft. S
Ualleye Pollock
.07u
.93
.OOSu
.016
.09
.39
.42
.09
.25
.07u
.Olu
.04u
5.3
46056
Ualleye Pollock
07u
.60
• OOSu
.011
.07
.56
.68
.06
.35
.07u
• Olu
,04u
6.3
46057
Ualleye Pollock
.07u
.93
.OOSu
.016
.07
.44
.45
.08
.40
.07u
.Olu
04u
6.0
46059
Pacific Hake
07u
.47
.0G5u
.011
.23
39-
.50
.01
.30
07u
fllu
04ii
1.3
46060
Pacific Hake
07u
.57
• OOSu
.011
.06
.39
.78
.02
.20
. 07u
.Olu
¦ 04u
5.3
46061
Pacific Hake
07u
.33
.OOSu
.013
.09
.S3
.45
.06
.30
,07u
.Olu
. 04u
5.0
46062
Pacific Hake
07u
.40
.OOSu
.011
.11
29
.16
.13
.30
.07u
.Olu
. 04 u
4. J
46063
Pacific Hake
.07u
.90
• OOSu
.01)
.11
.27
.57
.10
.1?
,07u
Olu
• 04u
5.0
-------
Tjble 8-3. cont.
ft*Hi Concentrations Found in Fish Muscle Tissue Stapled *t the Point Defiince Dock, ug/9 wet weight
l*t). No.
Species
Sb
As
Be
Cd
Cr
Cu
Pb
«9
Hi
Se
*9
T1
In
28000
Rock Sole
,07u
2.5
.003
.006
.28
.40
.45
.05
.36
.25
.006u
• 05u
8.8
29000
Rock Sole
,07u
11.9
.003
.008
.24
.47
.45
.03
.60
.18
. 006u
.05u
8.8
29001
Rock Sole
.07u
34.1
.003
.008
.20
.35
.55
.02
.50
.29
.006u
. 03u
9.8
29002
English Sole
,07u
14.6
.003
.012
.20
49
.72
.03
.43
.17
. OOSu
.03u
7.0
29001
Enttlish Sole
,07u
3.2
.003
.004
.22
.42
10.4
.03
.40
.17
.006.J
. 03u
7.3
38027
English Sole
,07u
7.9
.003
.004
.41
.27
.67
.02
.73
.06u
.0071/
.04u
5.3
2<>094
C-0 Sole
,07u
37.8
.003
.006
.18
.44
.65
.02
.50
.06
.006u
.03u
10,7
38028
Buffalo Sculpin
,07u
3.6
.005u
,005u
.19
.37
.40
.02
1.0
. 06 u
• 007u
.04u
10.7
01000
Uilleye Pollock
.07 u
2.2
. 004u
,004u
.14
¦5?
.25
.07u
. 007u
.Oiu
4.7
01001
tolleye Pollock
.Oiu
1.4
.G04u
004u
.11
.85
.61
.02
.40
.07u
¦007u
• 04u
4.7
01002-
Uilleyi Pollock
• 06u
1.9
004u
.004u
.08
.85
.63
.02
.22
. 07u
007u
04u
4.7
01003
Uilieye Pollock
• 06u
1.3
.004u
,004u
.11
.85
.63
.02
.19
,07u
.007u
,04u
5.3
01005
ttalleye Pollock
.OCu
.40
004u
,004u
.12
.85
.63
.08
.22
.07u
007u
.Oiu
6.0
01004
P*cific Suglura Sculpin
.Oiu
.87
,004u
,004u
.12
1.2
.67
.03
.22
07i,
,007u
. 04u
8.3
01006
Pacific Sughorn Sculpin
.Oiu
.67
,004u
.004u
.12
.66
.42
.03
.12
.07U
.007u
.04u
8.7
OIOO;
Pacific SUghorn Sculpin
.Oiu
.60
• 004u
.004u
.08
.80
.49
.07
.25
,07u
,007u
,04u
7.0
-------
Table 8-3.cont.
He til Concentrations Found in fish Muscle Tissue Sapled at the Old Town Dock , ug/g wet weight
Ub. Mo.
Species
Sb
As
•e
Cd
Cr
Cu
Pb
Hg
Hi
Si:
*9 1 T1
Zn
28001
¦ockfish
¦ 07u
.55
.003
.012
.28
66
.58
.03
.48
.14
. 006u
.04u
9.8
28002
Pacific Staghorn Sculpin
.07u
.31
.003
.012
.25
.72
.72
.03
.90
.12
.006u
.04u
10.0
2900?
Buffalo Sculpia
•07u
.13
.005
.004
• IS
.43
.58
.02
.43
,06u
.009
.03u
11.0
29006
Rock Sole
07u
l.S
.005
.006
.14
.39
.81
.03
.50
06u
. 006u
¦ 03u
6.B
44006
Rack Sole
¦ 07u
7.a
•006u
.009
.28
.41
.33
.01
.27
.08u
• Olu
• 04u
6.0
29008
English Sole
• 07u
3.2
.003
.006
.13
.40
.49
.02
.45
.12
.006u
• C3u
6.0
29009
English Sole
-07u
2.1
.003
.008
.IS
.3S
.55
.04
.48
.15
,006u
• 03u
5.5
29040
English Sole
07u
3.4
.003
.006
.13
.40
.69
.02
.44
.09
,006u
• 03u
6.3
4907S
Walleye Pollock
,07u
1.1
,006b
.006
.09
.41
.31
.11
.20
.07u
.Olu
• 04u
S.7
49076
Walleye Pollock
07u
3.4
,00Su
.006
.12
.52
.31
.10
.17
.07u
.Olu
.04u
5.0
49077
Walleye Pollock
• 07u
1.7
.OOSu
.006
.07
.70
.31
.04
.27
.07u
.Olu
.04u
4.7
49078
Walleye Pollock
.07u
1.8
,005u
.004
.07
.59
.42
.04
.37
. 07u
.Olu
. 04u
5.3
49679
Walleye Pollock
,07i
1.4
.OOSu
.006
.07
.81
.36
.12
.33
,07u
.Olu
• 04u
6.0
49080
Pacific Cod
,07u
3.1
.OOSu
.004
.07
.52
.42
.12
.40
. 07u
• Olu
¦ 04u
6.0
49C1
Hike
• 07u
.5
.OOSu
.004
.08
.33
.36
.07
.17
07u
.Olu
. 04u
4.0
4906?
Pacific Hake
.07u
.8
.OOSu
.004
.08
.33
.31
.09
.20
07u
.Olu
.04u
S.O
49083
Pacific Hake
.07u
.9
• OOSu
.004
-OS
.41
.33
.13
.40
07u
.Olu
.04u
4.3
49084
Pacific Hate
•07u
•S
.OOSu
.004
.OS
.41
.39
.11
.33
,07u
.Olu
.04u
4.7
49085
Pacific Hake
,07u
.S
.OOSu
-016
.06
.67
.36
.11
.33
,07u
.Olu
.04u
5.7
-------
Title 8-3. cont.
He Hi Concentrations found In Fish (tiscle tissue Stapled at Discovery Bay, 119/9 wt Melght
Lab. No.
Species
Sb
As
Be
Cd
Cr
Cu
Pb
Hg
Nt
Se
Ag
T1
In
40000
Surry Flounder
¦ 07u
.70
.OOSu
.013
.48
.35
.61
.05
• lb
.17
.007u
04u
4.7
46005
Flathead Sole
.07u
S.3
• OOSu
.004u
.07
.28
.31
.04
.20
.07u
.Olu
.04u
4.0
48014
Pacific Staghorn Sculpin
.07u
2.8
.OOSu
.004
.12
.52
.27
.02
.30
. 07u
.011
04u
6.7
48015
Pacific Staghorn Sculpin
• 07u
1.7
.OOSu
.006
.12
.44
.45
.04
.30
.'J7u
.Olu
04u
6.7
48016
Pacific Staghorn Sculpin
.07u
1.7
.OOSu
.004u
.09
.33
.42
.04
.23
07u
.Old
.04u
5.0
48017
Pacific Staghorn Sculpin
.07u
3.1
.OOSu
.004u
.14
.48
.39
.04
.36
• 07u
.Olu
,04u
7.3
48018
Pacific Staghorn Sculpin
.07u
.9
.OOSu
.004
.09
.48
.31
.02
.13
.07u
.Olu
,04u
7.3
48019
Pacific Tcncod
.07u
3.4
.OOSu
.006
.Ob
.40
.42
.04
.13
07u
.Olu
04u
S.O
48000
English Sole
.07u
2.3
.OOSu
.006
.07
.40
.49
.04
.20
. 07u
•Olu
- 04 u
5.3
48001
English Sole
•07u
2.1
.OOSu
.006
.OS
.44
.61
.05
.26
,07u
-Olu
,04u
7.0
48002
English Sole
.07u
4.1
• OOSu
.004u
• OS
.40
.46
.02
.33
• 07u
.Olu
.04u
4.3
4800}
English Sole
• 07u
3.6
.OOSu
.008
.07
.44
.31
.05
.17
. 07u
.Olu
.04u
4.7
48004
English Sole
.07u
4.0
.OOSu
.004u
.07
.40
.45
.04
.17
. 07u
.Olu
.04u
4.7
4800/
Ualleye Pollock
.07u
.9
.OOSu
.004u
.12
.93
.31
.03
.26
.07 u
.Olu
04u
4.7
48008
Ualleye Pollock
.07u
1.3
• OOSu
.006
.05
.33
.31
.09
.17
.07u
.Olu
.04u
4.7
48010
Ualleye Pollock
.07u
.9
.OOSu
.004
.05
.48
.39
.11
.17
.07u
.Olu
04u
4.7
48012
Ualleye Pollock
.0/u
4.1
.OOSu
.004u
.07
.59
.33
08
.23
.07u
• Olu
• 04u
6.7
4801)
Ualleye Pollock
.07u
1.5
.OOSu
.006
.07
.33
.42
.08
.23
.0?u
Olu
.Wu
S.O
-------
APPENDIX C.
Comparison of Organic Compounds Among Fish Species and Sampling Sites
NOTE: "b" indicates that a concentration falls b#*v*en the detection
level and the quantitation level for that compound
" « " denotes that quantifiable concentrations have been averaged
together Mlth "m" concentrations
For more Information concerning detection limits see Appendix F
page 115.
-------
Table C-l. The Average Concentrations of Priority Pollutant Organic* Identified In Fish Muscle Tissues
Saapled at Four Co—enceaent Bay Sites and a deference Station at Discovery Bay
* • ug/kg wet valght »* - ug/g ml valght
Compound
Nd/Na
J
B
H
0
Nd/Na
X
¦
N
0
Nd/Na
I
B
N
0
Nd/Na
t
B
H
0
Nd/Na
if
B
N
0
kexachloroberueae *
4/9
<48.25
3
1
0
tsophorooe *
1/12
920*
0
1
0
naphthalene*
1/11
S10
1
0
0
.
n-ni trosodlphenylaalae *
1/12
64
0
0
1
bls(2-etbylfceiyl) phthalate
6/10
281
5
1
0
6/11
111.67
6
0
0
7/12
122.14
1
2
4
6/9
62.67
2
4
0
2/5
125
2
0
0
butylbenzyl phthalate *
2/10
385
1
1
0
2/9
54.5
0
2
0
di-a-butyl phtlMlate *
2/10
595
1
0
5/11
598
S
0
0
5/12
342.4
1
0
4
6/9
266.7
3
3
0
2/5
120
2
0
0
diethyl phthalate *
6/ 10
87.5
5
0
1
8. .
* 33.38
7
0
1
10/12
67.9
4
3
3
1/9
35.29
5
2
0
2/5
"54
2
0
0
dlaethyl phUalate *
1/5
8
1
0
0
phenol *
2/10
141.S
0
0
2
1/11
22.67
0
0
3
l.l.l-trlchloroethaae "
2/16
0.04
0
1
1
**
t.2-trans-dlchloroethylene
2/22
0.01a
0
0
2
ethyl benzene "
1/22
0.01a
0
0
1
1/19
0.01a
1
0
0
•ethylene chloride **
aj 16
0.48
0
3
5
2/22
0.18
0
0
2
3/19
0.53
0
0
3
1/ IB
0.1
0
0
1
tetrachlorocthylene "
7/11
"0.033
4
3
0
toluene **
1/16
0.03
0
0
1
«
1/ IB
0.01a
1
0
0
trlchloroethyleae ••
6/11
<0 043
4
2
0
(Md/Ma)a Mo. Fish with Oetectlble Values over No. Fish Analyzed (B)-Bottoa Fishes (Hj-MUed Fishes (0)"0ff-Bottoa Fishes
-------
M
*
A
m
**
u
m
k.
TtfcU C-2. £
CoMMtritlM of Priority -•
folliitMlt found In Fiilt *
Huscl* Hum Stapled it: %
2
Mylebot Iteterwiv •
Lrt. Mo. VP. Code "
•
it
«
**
c
S
E
o
.c:
u
«
K
~
m
c
o
L.
o
-C
a
o
M
#
t
<*
«
.c
a.
2
«
C
I
K
C
*
£
O.
5
o
•*
e
c
•
c
•
<•
£
•#
-C
a
>*
K
•»
£
5
r
r*
M
2
*
•»
**
•
1
**
c
a.
">s
*4
C
2
•
S
a
2
£
a.
"as
**
a
a
*
c
i
U
•
•
w
«
2
¦Ml
a
-5
•
•
«-*
2
a
f
a.
>.
5
|
T>
«•
*
<•
u
c
p
K
Ui
u
«<
•
8
*
a
.A
«
m
o
»
*
#
Af
C
**
8
ft.
o
fT—
w
X
•
* *»
• C
*
*>,
£.
i
js
£
u
TJ
w«
C
«
fc.
4J
t
C|
«
•
•»
C
«i
N
C
X
>*
•C 4
*
•
•
«
X
o
-C
u
2
*
4J
•
6
i
•
»
*
*
>>
.c
•
e
c
£
o
«
k.
4-»
5
•
i
s
8
•
t
c
•f
>»
.c
«J
8
U
o
f
u
t.
w
16051 (2)
so
«0
~so
40
0.05a
0.05a
J60S2 (2)
so
114
47
0.05a
0.05a
160S3 (2)
34
yt&A (2>
600
SO
0.05a
0.05a
J60S5 (2)
«2
290
X
0.05a
0.05a
44 00 (9)
31a
72
12
71
2S
0.05a
44001 (9)
0
0
0
0
0
0
0
0
0
0
44002 (9)
42
SO
C.Ola
0.01a
44003 (1)
26
44004 (7)
U
37
134
IS
0.01a
44005 (7|
0
0
0
0
0
0
0
0
0
0
0.01a
(s)« a*'
! Neutr
¦ 1 or /I
:ld £«l
•ctibl
i frtcl
ions Na
[ Am 1«
red foi
(*)• u
> Neuti
il or i
Id Cat
Willi
• Coapi
«d Exp
cssed
in uq/I
I wt
etifct
Utile
laapouii
1 Capri
.ted In
"9/9
Htl 1M
0>t
(2)« Catllsk Sol*. (7|- 'ttlftc SUghorn Sculpt*. (9)- UMtespotted Creeallng
-------
«*
A
labia C-2. coot. 3
U
Concentrations of Priority £
Pol)ut««ti found In fish 2
hsdc Tissue Saapled at: ^
m
i.
a
City Waterway ^
ft*
«A
m
Lab. No. sp. Coda *
•
t
*
M
C
•
I
0
JC
u
*
1
•
ftl
s
L
o
c.
a.
o
1*
«
i
•
m
5
-c
tx
m
c
*
«*
c
1
>1
e
i
a
o
o
£
c
•
c
*
m
c
£
a
»»
m,
»
£
r
N
«*
2
•
«
m
5
c
a
N
c
1
3
Ji
•
ft*
«i
m
5
¦C
CX
*>
3
Ji
•
C
•
V
~
m
s
£
a
•
V
•
s
m
2
C
0.
>»
£
*-»
I
5
«*
ftl
*o
t
o
a
h.
*«
M
hi
*o
u
<
«
8
£
o
«rt
ft*
<•
O
>
•
•
*
c
«
£
w
8
i.
0
JZ
u
1
*-<
1
• —
• c
ft»
>%
£
6i
a*
o
u
o
Xi
u
Y
•A
c
«
u
*»
rvi
*
•
•I
c
ftl
N
C
4»
.O
*>»
£
*»
*
•
u
o
x:
O
fti
c
ft*
>.
£
~
•
ft*
c
«
>»
£
«J
ftl
o
l_
o
£
U
u
a-*
ft*
4-*
•
t
c
ft*
3
O
•
1
ft>
*>»
£
8
L.
O
.C
u
fc.
29UOS (12}
0
0
0
0
0
0
0
0
0
0
46051 (12)
A
0
0
0
0
0
0
0
0
0
46G52 (12)
0
0
0
0
0
0
0
0
0
0
0.01a
0.01a
38022 (5)
160
580
35
44050 (10)
0
0
0
0
0
0
0
0
0
0
46058 (|0)
0
0
0
0
0
0
0
0
0
0
38026 (3)
430
30
36050 (2)
170
&80
45
36021 (2)
1B0
700
50
38021 (2)
60
30
38024 (2)
80
700
35
38025 (2)
510
20
25
460S 3 (13)
0
0
0
0
0
0
0
0
0
0
46054 (13)
35
0.3
46055 (13)
0
A
0
0
0
0
0
0
0
0
46054 (11)
0
0
0
0
0
0
0
0
0
0
0.01a
0.07
4605? (13)
46059 (11)
21
46060 (11)
0
0
0
0
0
0
0
0
0
0
46061 (II)
17a
12
46062 (II)
0
0
0
0
0
0
0
0
0
0
46063 (11)
0
0
0
0
0
0
0
0
0
0
(2)- foflls* Sola . (J)- flatted Sola . (5)- Surry Flounder . (10)- Pacific Cod . (II)- Pacclflc Haka . (12)- Pacific Toaco<1 (U)' Uallcye Pollock
-------
«
JZ
s
u
•
Table C-2. coat. «
Concentrations of Priority_
hllntMb Found la Fist ;
hscl« Ilitw Saapled at: 5
2
014 To— Dock S
Lafe. Mo. sp. Co* "•
•
?
•
*4
C
I!
8
0
X
W
m
M
1
~
t
O
L.
O
C
a
0
•M
•
|
1
X
a.
2
#
•
C
i
?
a.
-0
e
e
~»
c
«
c
•*
m
m
X
*-•
x:
0.
>,
K
•
x
>»
x
*>
t
CM
M
a
•
••
•
2
W
X
O.
S
C
8
X
M
9
A
•
«*
*>
2
«-•
£
d.
•
c
•
V
•
m
«-»
a
X
0.
•<
i
•
m
¦++
m
2
~*
X
A
>.
X
U
|
•5
w»
•
A
«
«-»
u
c
K
Iftl
T>
O
<
•
2
*
a
1
M
m
4*
m
O
>
•
c
«
X
«->
s
1-
0
X
u
L.
*>
•
w*
w*
;ll
>»
il
%t
0
U
O
-C
U
7
M
c
«
L.
At
•
N
*
•
•
«P
C
*
C
2
>»
X
+*
m
*
•
«
L
e
x
0
4»
C
•*
5
w
1
~
~
*
*>»
X
*
t
e
X
0
•
s.
*J
*
*»
•
I
c
s
0
•
I
e
*
X
*>
s
u
0
X
0
L.
?aooi
(6)
M
24002
(7)
920a
190
130
24007
(t)
140
70
29006
(4)
140
9S
MOOt
(4)
0
0
s
0
0
0
0
0
0
0
0.01«
29006
(?)
63
29009
(2)
1300
120
29040
Hi
60
490/5
(HI
0
0
0
0
0
0
0
0
0
0
490/6
(13)
0
0
0
0
0
0
0
0
0
0
490/7
(D)
0
0
0
0
0
0
0
0
0
0
490/1
(I3J
110
100
6
490/9
(1JI
190
110
49000
(10)
S9
62
/
49081
(U)
64
26
140
13
49062
(U)
0
0
0
0
0
0
0
0
0
0
-.906]
(111
0.7
49064
(HI
0
0
0
0
0
0
0
0
0
0
0.)
49065
(111
•
0
0
0
0
0
0
0
0
0
0.6
(o».
late Neutral or
eld t.
ractlb
Midi ¦
t Anal
»d Fo
<«!•
Base Neutral or
<14 ix
ractlb
reued
a w*t
cllht
CM-
Volatile CoafKu
d
[
-------
«A
•»
•
lable C-2. coat. u
Concentration* of Priority~
Pollutants found in Mih J
KiSclt Tissue Sampled at: _
k.
¦a
•»
*oint Defunct Dock 1
«*
Lab. »>. ip. Code "
•
If
•»
N
c
»
A
8
O
c
0
«
M
1
•
a*
c
u
k
o
£
CL
o
Mt
•
*
•>
5
£
a
8
•
*
c
E
c
*
X
a.
•o
o
e
•j
*c
•
c
•«
£
**
x:
e.
X
M
*
£
X
X
«*
*
CNd
W*
X
•
•»
•*
m
m
c
+*
x
a
">»
p*
c
%
>.
a
x
•
«•
+*
m
2
X
o.
>*
ia
c
i
V
t>
•
m
*•»
2
*»
£
a
*
~*
•
•1
*»
m
m
x:
**
x
A
*>»
X
|
v»
tl
X)
m
«-»
U
«
i.
**
K
Ui
X)
u
4
«
o
c
*
a
«•
<•
o
«
•
«
£
s
w
o
£
u
L-
«-»
t
• t
ft e
">»
X
A*
o
L.
0
X
u
¦U
1
4A
c
?
<-*
Od
•
•
*
c
41
N
c
*>»
X
»
•
•
«
I
o
£
u
*
tl
~-»
e
•
•»
*>»
£
«*
o
u
o
X
o
m
i.
*-»
«*
«-*
•
i
c
1
o
4J
•
I
>*
X
•-»
8
L.
o
X
u
k
ittMO (4)
2S0G0 (4)
230
m
root (4)
24002 (2)
230
86
2«003 (?)
306
33
3002; (2)
170
75
770
40
(1)
490
156
iao?a (i)
260
695
420
01000 (13)
0
0
0
0
0
0
0
0
0
0
0.6
01001 (13)
c
0
0
fa
0
0
C
0
0
0
0.4
0.03
010112 (11)
0
0
0
0
0
0
0
0
0
0
0.4
0100) (Hi
40
63
0.03
0.4
OIUOS (I))
220
0.6
01004 (0
0
0
0
0
0
0
0
0
0
0
O.OS
0.4
61006 (?)
0
0
0
0
0
0
0
0
0
0
0.7
11100/ 11)
0
0
0
0
0
0
0
0
0
0.3
({)• ku Neutral oi
Acid {
tract!
It Fra
l ton H
t Aiul
zed Fo
(')- hit Neutral ui
At 13 [
.tract!
le Con
ound I
pre lit
i in u<
k9 w
Mlgll
(••)• Volatile Co|j
'ltd f»p
*»*ed
1 u^/9
W( w
ignt
(>)' C-0 ioit, (2)* Sole, (<)• duct Sol*. (6)' iluffjio Sculpln, (?)• Pacific Sltyhorn 5c_)pln, (13)* MalUye <,ollock.
-------
«A
m
%
m
«-*
u
«
Table C-2. coat. $
Ui
Concentrations of Priori tyJ
Pollutants found In fish |
Muscle Tissue Stapled at: *
Discovery Bay £
Lab. Ho. sp. Co<>e 10
•
?
%
N
e
s
e
o
_c
u
m
H
•>
-C
•
i
c
o
t-
O
£
O.
O
«•
»
2
m
s.
£
2
•
c
1
1
£
a.
•5
o
M
E
c
•
c
«•
m
m
£
«-*
a.
1?
*
.e
p
**
r
(M
A
•
a
«
m
.c
**
£
a.
N
C
X
a
•a
~
3
m
5
c
GL
£
•
C
1
"O
1
•
3
m
»"»
s
*»
.£
Q.
p
*»
V
¦o
•
—
•J
m
£
£
I*
«-*
1
«•
1
r—
m
**
o
m
%.
w
M
ui
TJ
u
<
ft
c
£
a
w*
•»
m
o
>
•
ft
2
m
£
s
c
o
£
u
c
•
m
•¦4
ft c
»
5
•»
e
o
c
u
?
*
c
c
*»
IX
**
ft
ft
*
e
*
c
>»
£
«-»
ft!
ft
4
t
o
.e
u
2
*
•j
e
•
\
%
*>»
£
*
e
o
£
o
m
w
**
•»
**
•
3
o
•
*>»
«-•
8
ft.
O
x:
u
i.
40000 (S)
0
0
0
0
0
0
0
0
0
0
4800S (3)
0
0
0
0
0
0
0
0
0
0
0.01*
48014 (7)
0
0
0
0
0
0
0
0
0
0
4801S (7>
0
0
0
0
0
0
0
0
0
0
40016 (7)
0
b
0
0
0
0
0
0
0
0
4H017 (7)
0
0
0
0
0
0
0
0
0
c
48018 (7)
0
0
0
0
0
0
0
0
0
0
48019 (12)
0
0
0
0 •
0
0
0
0
0
0
48000 (2)
0
0
0
0
0
0
0
0
0
0
40001 (2)
170
140
79
8
48002 (2)
0
0
0
0
0
0
0
0
0
0
48003 (2)
80
. 100
38h
48004 (2)
0
0
0
0
0
0
0
0
0
0
4U007 (13)
4WNM <131
0
0
0
0
0
0
0
0
0
0
48010 (13)
0
a
0
0
0
0
0
0
0
0
48012 (13)
48013 (13)
0 1
(0)- Base Neutr
1 or A*
id Exti
ctible
rractlc
11 Not
foalvn
1 for
(*)< Base Neutr
1 or A<
id Exti
JCtibli
Conpot
id Exp
essed
it uq/k
wet vt
gKt
(••)» Volatile C
¦pound
Expres
ed in
9/S "•
: weigh
:
(2)* [ajllsk Sole, (3)* flathead Sole, (5)* Starry Flounder, (7)» Pacific Staghorn Sculpin, (12)* Pacific Toacod. (13)* Walleye Pollock
-------
t
2
**
fable C-2A. t
Concentrations of Priority"
Pollutants »
*
*
£
H
£
r
»
M
c
>»
£
•
4-«
«
5
X
a
'x.
~j
•
c
t
•
m
2
x
a
">»
£
•5
~
*
2
*4
X
a.
>»
£
«-»
I
¦5
£k
«-*
u
«
t-
*1
K
Ui
•o
u
«
•
o
c
£
a
•I
•
o
>
•
•
*
c
£
L
O
X
u
k.
t
•
«
w
c
•
">s
X
**
!
o
x
u
"O
¦
•
•
»
b
C
o
ft#
o
ft>
•
~
«
X
o
£
o
ft/
c
ftf
»>
£
«-»
ft;
s
~
•
ft*
C
•I
>s
£
ft>
O
L.
o
£
U
M
i.
ftl
•
t
c
3
C
«
L
C
o
>»
X
£
L.
C
X
u
L.
W
Hylebos Waterway
36058
0
0
0
0
0
0
0
0
0
0
0.01a
0.01a
36059
0
0
0
0
0
0
0
0
0
0
0.01a
0.14
0.01a
City Waterway
36056
0
0
0
0
0
0
0
0
0
0
36057
0
0
0
0
0
0
0
0
0
0
Olscovery Bay
48006
0
0
0
0
0
0
0
0
0
0
48011
0
0
0
0 '
0
0
0
0
0
0
(a)- Hoi
Analyz
id For
~y GCHS
but Hi
Oetec
ed on
C/EC 1
Fish
nd Cri
is from
the Hy
ebos U
terwty
as She
m In I
ibles A
2 and
1-3
(0)- Bai
e Neutt
il or t
:id Ext
ictibl
t frict
ions Nc
t Anal*
fed Foi
<*)•
a Neutr
il or A
:id Ext
-ictlbl
• Coapc
md Exp
-essed
in ug/l
j wet
eight
{")• »«
ladle
:oapounfi (xpre
Jed Iq
ug/9
wet Ih
,ht
-------
Title C-3.
Non-Priority Pollutant Or«Mic
Coepoueds Tentatively Identified
in Fish Muscle Tissue Stapled at:
|
I
Nylebos Waterway
Lab. Number Species
1
S !
m
CL
o
I s s £
g 2 V -o* ?
2
~>
2
£•
I
¦o tj m ^
• • -c
m n n v
• • • «
E O. N N
f |*S„f
I M N ^ N
e ^ § c w e
i jss*^
t
«*
N
C
1!
C V c
a 6«
>» X 2
?; ?
• 6 «•
N T M
e ^ e
s ~ s
*
•*
M
c
2
2.1
•>>,•»>.
N£ N £
c «-» c *-»
l» ^ «
36051
English Sole
36052
* *
360S3
• m
360S4
m m
H
360S5
4t m
44000-
Uhitesp. Greenling
44001
-
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
44002
-
44003
Pic. SUg. Sculpin
44004
-
44005
.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
| Compound Detected
( | Cexpound Hot Detected
| Q j Base Neutral or Acid Cxtractable expound Not Determined
-------
I Mil' I'N
iwnnq
i tui'inq
• [»uedOJd
»uou»;d»M i
auourjdaq
»uou»i»m-c
-1/Cm;»ujP
•>'2'auou»iu»d-E
-|/Mj»w-fr'»uoimuad-2
• i/Cm»u-£'Juou»iu»d-2
»uoutiuad-2
luourjnq-g
»U01»3*
-iXmh
-S*»uou»jn>.(HS)2
-l/Ciu»d-?'-«jn>
it* t/m» ,iKi»a«-t'p^»
3ll>(xoq.it3-auappa4id-2
ja;s»
'pp» 3(
-------
Table C-3.
cont.
Hylebos
Waterway
lab.
«-• O
l 3 •»
(M — N O
~.2;
. *.
*>»
o
t
o
•
r>
|
£
•o
m
*
$
i
T3
"O
*
»
•
i
vO
-
« o
o
o
K
m
<«
«A
Vv
o o
u
U
5?
£
>»
U A
u
u
4
•
•
£
|
u
N
(V
N
CM
"i. £ *
i
t
36051 1 | | | |
36052
¦
T,*?r
36053
36054
36055
44000
44001
0
0
0
0
0
0
0
0
0
0
1
44002
1
1
44003
44004
44005
0
0
0
0
0
0
0
0
0
~
i
1
nj
Zj
1
1
#
:
J
-------
Title C-3.
Non-Priority Pollutant Organic
Caapounds Tentatively Identified
in Fish Muscle Tissue Saapled at:
City Waterway
lab
2
e
to
P
*
X
Species
g
s
~*
m
s
«
N
e
£
2 i
o c
^ ?
O «-»
X I
o •
o
-o u
N T>
C >»
4 5
>t
jC
+*
S
.c
4-»
8
»<« M
2! *4
«
N
C
21
£•
~>
I
TJ
I
rt
«-4
£
*
C
£
>»
.C
4-»
g
!•
*«»
•1
?
CM
t
~
*
1
1?
I"
i
£
4-»
•»
««
1
«
I
«/
j
*>»
«•
e
««
N
C
«
N
>»
£
**
•1
C
ft»
N
«>
c
N
£
£
1
e
£
C
n
ft*
e
c
JS
C
£
—
2 t.
C *-> C
* >»
N
c •
£
2900S
Pacific Toacod
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
~o"
0
0
0
46051
m m
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
T
0
46052
m m
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
rT~
46050
Pacific Cod
0
0
0
0
0_
0
0
0
0
0
0
0
0
0
~~F
0
0
~0~
0
46058
» «
0
0
0
0
0
0
0
0
0
0
j_L
0
0
T
0~
a
0
~0~
0
3802?
Starry Flounder
38076
Flathead Sole
_
36050
English Sole
38021
« m
1
38023
m m
Zj
38024
|
¦ !
— " 1,1 1
B
38025
-
1 Efl
m
8£
K
sm
46053
Ualleyc Pollock
0
0
0
0
0
0
0
0
0
0
~o"
0
0
0
0
0
0
0
0
46054
-
kg
4605S
M •
0
0
0
0
_0_
0
0
0
0
0
0
0
0
0
0
0
0
0
46056
¦ a
0
[()
0
0
0
0_
0_
0
0
0
0
0
0
0
0
0
0
0
0
46057
« •
46060
Pacific Hake
~0~
1 °l 0
0
~T
~~c
0
~0~
~cT
~0~
0
~0~
0
~~0
-------
. iXioau-f (vuv i""|
IfUPinq
- t»upj0Jd
au0u»)d3M 2
~uoueiaaM
»uo-2-u»*jm-£
juou»*»n-c
-f'2'»uousjuJi)-c
• »uout)u»d-2
• iXqjfcu-C auout)u»d-2
»uounuad-i
auoulinq-;
»L01»0I
•lW
-5'»uoutjn>-(HS)2
-l^)uad-z'u*jn>
)t» i&n» •ixjast-ppp*
3i (X*oqjt3-»ujpi j*did-J
J»U9
•pp» 3}0tP*Utx»M
Jljfi i/qiiv
'ppt 3(0u»«»y
J»1S»
'ppt 3|0utiu*d-2
•pp» 3tOU»1U»d
j»u» i*m*
•po» 3(0u*>nq
j»;s» iXw^w
• 'pot 3*ou«nq
J»U» 1/141 »UI
• i^i»ui'2 'Pts» 3)0u»)/iq
-------
I
VO
o
— o
w u •
WON
libit C-3. 'no «
cent. -• ~ — o -»
City ~ V -if ~I5
•tetervay g ~ g 5 g g
m cvi av
r *c« h l
Lit. Niaber J - «J- £ "
M X
• o
JC
O **
22
•f «-»
x m
Z "Z 2
e c
s. "5
u -»-
^ 7
:«
>>
r- C II
>* ** C
.£•»¦»-
w e m
• • «
E .c fc-
29005 | | 0 |
0
0
0
0
0
0
0
0 U
0
1 1
46051 —
0
0
0
0
0
0
0
0
o wm
0
4£06?
0
0
0
0
0
0
0
0
0
0
46050
0
0
0
0
0
0
0
0
0
0
46058
0
0
0
0
0
0
0
0
0
0
38022
38026
36050
1
38021
i
38023
H
z
38024
38025
n
_
46053
G
0
0
0
0
0
0
0
0
¦£ri
0
z]
46054
ss
¦WW
SB
k
1
1
i
46055
0
0
0
-id
ol
71
0
0
_0
7v
0
j
1
46056
0
0
0
0
0
0
0
0
0
0
46057
1
II
46060
o
0
0
0
0
0
0
0
0 Ha
0
46061
LZ
' f' ¦
rz
46062
0
0
0
0
0
0 .
0
0
h>
0
46063
0
0
0
0
0
0
0
0
EH
0
—
46059
\—W
-------
Table C-3. cont.
Hon- Priority Pollutant Organic
Coapounds Tentatively Identified
in Fish Huscle Tissue Saapled at:
Old Town Dock
Lab. Ikaber Species
? S
6 •
« -©
c
g
s
c
£
•
X
1
£
tt •
5 2
>»
5
|
~ I
•
>»
p
>»
T "f
o c
•
«* .c
e
1
•»
E
X
f
—
*>
*>
ft*
ft»
>s
'ss >.
c c
r- w
c ~*
^ f
V
n
»¦>
.C
£
"O
•D
"O
c
c
ftt ft!
X £
o •
— o
N
rt
CM
*>
«»
«< •»
« «
ft»
<
it
CM
A
CJ
« ft)
t »
N -O
c X
9> -C
Xu i
*
•
c
*
c
•»
C
4» »~ •>
C >» C
ft*
C
r
«-»
ft»
2
*
c
s
sz s.i
A >«
• >«
o« v
N N
•
N
•t
N
«
•4
»
fx
ft. .C ft>
N W N
4)
M
6
ftl
M
*
ft)
N
ft;
•4
«> >v fr >>
N£ N £
CMC
c
J!
c
2
c
m
A
C
5
c « c
* E 1!
C
£
—
c
£
C
2
c
ft>
c
ft*
-O
c ** c «-»
tj ^ «
28001
Rockfish sp.
r~
28002
Pac. Stag. Sculpin
!
L
29007
Buffalo Sculpin
1
i
29006
Rock Sole
•
44006
-
0
0
0
0_
0
0
0
0
0
0
0
0
0
0
0
0
0
29008
English Sole
¦
29009
• m
.H)v.
29040
m a
49075
Ualleye Pollock
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
49076
"
0
0
0
0
0
0
0
0
J
0
0
0
0
0
0
0
0
0
0
49077
m m
0
0
0
0
0
c
0
0
0
0
0
0
0
0
0
0
0
0
0
49078
m m
49079
m •
49080
Pacific Cod
49081
Pacific Hake
LI
49082
m m
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
~r
0
49083
m •
1
m
49084
m m
o 1 0
0
0
0
0
0
0
0 1
0
0
0
0
0
0
0
0
0
0
49085
-
0 I 0
0
0
0
0
0
0
0
0
0
0 1
0
0
a
0
0
0
0
£3 Coapound Detected ~ Coapound Hot Detected C3 Base Neutral or Acid Extractable Compound Not Determined
-------
£ I
a
so
ro
•
Table C-3.
cont.
Old Town Dock
lib. Ni*ber ^
V t- T) L T) «. i. « W <0 W
u (J W U A) <• ** O u «-*
• Ml « W « *> « tA
•» •» Ml U l» O If II
u u y « *¦ C U
O iso'x 0<- 2 >» * >, o
ecc£ £>«•£ cx c x:
bwowaxwwiiw « *-»
• - - - a. •# k ^
Hi
5 •
« E
CM
— • • I
u w u »— «—
«*!>»>»
W I* *j *<
u m e •* e
f •» u •»
o o « a
T) V L ^ N
% Sk. -O C
• X — «
k •* c. u u
«¦-« • • a
.c <\» «•-
x* S
28001 |
1 I 1 I 1
1 1 1 1
28002
29007
ffi
0
29006
<4006
0
0
0
0
0
0
29008
29009
29040
4907S
0
0
0
0
0
0
0
49076
0
0
0
0
0
0
0
49077
0
0
0
0
0
0
0
49078
49079
49080
E&
fct
m
49081
49082
0
0
0
0
0
0
0
49083
¦
m
49084
0
1,
0
0
0
0
0
0
4908S
0
EL.
f21
0
0
0
0
0
0
Kb
-------
to
u
a
U t
o
L. W l
Tftble C-3. Y §N.,
COAt.
Old Town
Oock
Ub. NuAer
N »• Ng
T O
~-a;
•M . * s-
• >* CM X » -t
CM £ • O M w
<~ £ • g
SI 1 r II
M k £ N U A
2
o
"5.
>»
•
?!
« •
-a
0
1
«n
£ ^
»
£
> f
«•
•
•»
• *D
X)
•>
"O
i «
-
o
X
m iii
u
M
&
& 8
*»
c
w
*
•
i £•
E
c.
M
CM
N
¦a
•
28001
1
V
2B002
29007
a
29006
44006
0
0
0
0
0
0
0
0
0
0
29008
29009
29040
49075
0
0
0
0
0
0
0
0
0
0
49076
0
0
0
0
0
0
0
0
0
0
49077
0
0
0
0
0
0
0
0
0
0
49078
Efl
is
49079
49060
49081
49082
0
0
0
0
0
0
0
0
0
0
49083
Sr
¦dj
49084
0
0
0
0
0
0
0
0
0
&l
L
0
49085
0
0
0
0
0
0
0
0
0
0
r
-------
Table C-3. cont.
Non-Priority Pollutant Organic
Compounds Tentatively Identified
in Fish tuscle Tissue Saapled at:
Point Defiance Dock
1
ai
Lab. Nuaber
Species
a h
o c
k £
£
1
>
>>
£
.C
Or •
V •»
¦C r—
£
]?
ft*
E
«*
. f
>»
>1
1
*>»
ft v
C
O C
«-» >»
K £
*-»
g
I
X
c
£
^ u
o *-»
M 1
o •
— o
•1
E
-o 1
U w
T)
«
CM
"O
t
n
OJ
V.
JZ
ftl
">» *>»
x; .c
4-* 4-»
r r
>,
JC
ft
1
ft
"O
N
x»
M
~%J
Or
X
ft
•> «•
-C -C
ft ft*
T> L
N -O
c >»
it
* M
«
u w
» L C
O ft ft*
N W N
•J
c
ftl
N
«i
c
ftl
N
ft*
C
ft
*4
ftl
c
ftl
*«
— «>
c >» c
ft -C ftl
N *-> N
ft
c
ftl
M
*>»
_c
•I
E
ft
C
a»
K4
•/
c
ftl
N
ft
C
ft
N
ft
c
ft
J? J. s',1
a» >* o >»
M -E «Si -C
C «A C
c
&
c
2
c
ftl
.n
c
€>
<£i
c ft c
S 6 J!
c
ft
n
-
c
ft
.c
e
5
C
ft*
A
e
ft
Xl
C W C
OJ U tJ tl
-£j -O
28000
Rock Sole
1
29000
• m
29001
m m
2900?
English Sole
29003
" "
38027
• *
K
2900*
C-0 Sole
38028
Buffalo Sculpin
-
01000
Hall eye Pollock KB
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
01001
-
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
01002
-
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
01003
*
fv
01005
" -
re,
- M
;S
01004
Pac. Stag. Sculpin
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
01006
.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0100/
-
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Q Compound Detected
| | Compound Kot Detected
| Q ] 6*&e Neutral or Acid Extracuble Compound Not Determined
-------
to
en
i
? ?
Title C-3.
coot.
>» >*
X .C
O +>
• il It
"O W
¦*- m • •
Vk« u
I ^ k u •
* •* w U • O If
Point Oefiftitce " »• "»- -Ji * o ^ >,
X_ . O >» C >s O «- c >**>>, o >. It K
Oock C£CfS>»«ccc C£ e «
~~ a. a A A a ^ -~-l AJ a> ai ai ^
u •—
>.
2
It «l U If
O Vfft
> • * >, >S
~# a* «¦» +*
ft, I
¦o «
^ I
L M
« •
o. *o
~ T J. <>i
2!
g
3
s
g
Lab. Muaber
*« ¦) w n w v c >i at>
9E9E9»«'6 • I
a i) £ a m
£ SS
1 £
N
S
-------
I
10
o%
I
_ n
Table C-3. ^ = ,
cont.
- W
Point Pefitnce K rsi
Dock £,0
4-/ » — C
r — • >, C£
— - >> cm x
(V X » © * •*
c v
-------
libit C-3. cant.
Non-Priority Pollutant Organic
Compounds Tentatively Identified
in Fish Muscle Tissue Saapled at:*
E
<«
Discovery lUy >»
I
lab. Nuaber Species X
I £
£ I
P
a-*
t
** +* >v
2
O • «•- «-•
- O U w
V L «
N "O
C »> L» «*
•» r i. e
A • o v v
• M N ** N
# c m c
_i .2 ".s
2
•»
N
c
£
C tl C
4 E 1!
* «
• £
It «
S I
M •
c
J! ~
£.1
o >> & X
m .c x:
C 4-» C *-»
* *»
40000
Starry Flounder
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48005
Flathead Sole
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48014
Pac. Stag. Sculptn
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48015
.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48016
m m m
0
0
0
*
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48017
mm m
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48019
Pacific Toacod
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48000
English Sole
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48001
« m
m
48002
» m
ofo
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48003
m m
m
48004
m •
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48007
Ualleye Pollock
48008
¦ «*
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
48010
m m
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
4801?
m m
• ,r ¦*
48013
•
I Compound Oetected
| | Compound Not Detected
| q j luse Neutral or Acid Extract*t»ie C««pound Not Determined
-------
T»ble C-3.
cont.
01scovery fUy
Lib. Number
40000
40005
48014
£
*1
E
CM
"O %.
•- *
u *•*
•O «/t
*0
u
15
*¦*
I»
Is
£
I
N
T3 i.
i E
X>
t~
u *
• *-»
u «
0 »-
c >,
•o .c
3 ft*
£3
1 o
0
0
*o
— 1.
U «l
«
U «f
o --
c >,
h c.
c «<
s e
"O
u
»
C £
•» **
O. *
» c
"O u
u «-»
« HI
w
o
o »>
C £
Jg
"O
« «
U tA
Q)
o
t< x
C *
> >
X £
O +>
¦O «•
u
>
* X
o u
•o «
«• 1
L. ^4
« •
a -o
c* u
« <•
w
'— ft*
CM
0
0
0
ft*
c
o
ft*
u
<•
Oi 2:
§ 8
c «
«
«-» c
3 •»
a a
»
£
«-»
1
n
ft»
e
o
c
>
£
O
f
V
a»
£
O
C
%
tO £
4-i
e a>
8 J
• TJ
»•>
0
0
0
%>
c
o
c
«
C
X
n
C
o
CM
c
M
*
n
0
0
0
*
c
o
e
*
a
-C
c
O
c
%
x:
1
CM
O
c
£
C
£
<
c
48015
48016
48017
48019
48000
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
J
=
0
0
0
0
0
0
0
0
48001
0
0
48002
0
0
0
0
0
0
0
48003
0
_
¦mm
48004
0
0
0
0
0
0
48007
d
48006
0
0
0
0
0
0
0
48010
48012
0
0
0
0
gH
0
•
0
0
...
48013
-------
to
*0
Table C-3. £ £ #
coat. r on
tM — NO 4
>» o
M •
Ota
£ i
w •»- c
*
o
e c
Discovery " " i-g
>*y t 2S S£ ?
« IM « ^ «
XT • C «t M V 2
l*b. Huaber - «~* S- £
y U 2
t- »»
O »'
• « U1
£ S
r • •»¦
C N
IS?
N ^ CM
40000
480%
48014
4801 &
48016
48017
48019
48000
48001
48002
48003
48004
48007
48008
48010
48012
48013
0 0
"n
-------
APPENDIX 0.
Biological Data
D~i
-------
Table D-i. Collection and Biological DiU for Fish and Crab Speciaens Taken in Coaaence*ent and Discovery Bays.
• P.A.
it. 113.
SuiioA
C
o
—
o u
•
* «~
•i —
a s
"Tiwe of
Collection
c
o
o —
T> U
O 1
£ •—
*< r—
Se °
77
Species
Sex
Fork
Length
<—>
Total
Height
(9)
Total
Fillet
Height
<9>
Liver EitmiuJ
Height Age
(9) lyrs)
Remarks
2SOOO
Point Defiance
Dock
7/15/81
1135
Hook »
Line
Hock Sole
Lepidopsetta bi 1 ineata
F
372
549.90
128.84
5.56
9
Posterior aargin of caudal
fin eroded.
28001
Old Town Oock
7/16/81
1840
Hook ~
Line
Rockfish sp.
Sebastes sp.
F
195
136.64
33.77
3.08
3
2eoo;
Old Town Oock
7/16/81
1945
Hook »
Line
Pac. Stag. Scu lp in
Leptocottus anaatus
F
224
167.38
34.75
6.64
4
29000
Point Oetiarce
29001
Oock
Point Oeiiince
7/20/81
7/20/81
1230
1230
Trawl
Trawl
Rock Sole
Rock Sole
M
F
198
195
76.02
101.32
23.91
32.43
1.00
0.91
4
3
Lesion on caudal peduncle,
bleeding with opaque area
surrounding it (blind side)
Blind side with bloodworms.
Oock
Blind side with embedded
bloodworais.
29002
Point Defiance
Dock
7/20/81
1230
Trawl
English Sole
Parophrys ve till us
H
280
162.03
43.73
2.71
4
Eyed and blind sides witn
e«t>edded bloodworais.
29O03
Point 0eM4nce
Oock
7/20/81
1230
Trawl
English Sole
F
234
113.41
30.30
2.52
4
Blind side uith embedded
bloodworms.
29004
Point Defiance
lock
7/20/81
1230
Trawl
C-0 Sole
Pleuronichthys coenosu'.
H
187
82.11
28.74
1.23
2
29005
Lit/ UateraMy
{(utea Harina)
7/24/81
IMS
Hook *
Line
Pacific Toacod
Hicroqadus proxiaus
c
179
46.21
12.88
0.54
2
29006
Old Town Dock
7/24/81
1015
Trawl
Rock Sole
M
226
151.17
49.60
3.36
3
2900?
01J Town bock
7/24/ei
1015
Trawl
Buffalo Sculpin
Enoptirys bison
F
285
615.75
65.97
22.46
-
Part of left aaxillary appeared
torn avay.
-------
Table 0-1. cont. Collection and Biological Data for fish and Crab Specimens Taken In Coanenceaent and Discovery Bays.
f.P.A.
Lab. No.
Station
o u o u
v II
2 = g =
9 9 TO
Sper ies
Total
Fort Total Fillot Liver Estimated
Length Weight Weight Weight Aye
Sex (m) (g) (g) (g) (yrs)
Remarks
idOOU
Old Town Uock
7/24/81
1015
Trawl
English Sole
H
267
153.45
48.21
3.20
3
:900S
lild Town Dock
7/24/81
1015
Trawl
English Sole
f
233
11B.48
30.41
1.82
3
:s(mo
Old Town Dock
7/24/81
1015
Trawl
English Sole
M
237
115.76
34.63
1.72
3
loOSO
City Waterway
(loten Marina)
9/10/81
1300
Trawl
English Sole
F
350
366.36
104.13
8.45
10
36051
Hylebos Waterway
9/10/81
1430
Trawl
English Sole
F
303
263.90
88.06
5.80
4
.>6052
Hyieiws Waterway
9/10/81
1430
Trawl
English Sole
F
282
197.58
68.95
4.95
4
-OI153
Hylebos Waterway
9/1U/81
1430
Trawl
Lug 1ish Sole
F
274
167.01
43.68
3.55
4
26054
Hylebos Uateiway
9/10/81
1410
Trawl
Lnglish Sole
M
265
161.91
55.14
2.50
-
= 5055
Hylebos Waterway
9/10/81
1430
Trawl
English Sole
M
258
160.49
50.59
3.42
3
j&056
City Waterway
(lutea (tan 114)
9/10/81
1300
Trawl
Dungeness Crab
Cancer aagister
M
181
868.06
217.53
-
-
•60S 7
City Waterway
(lutein Marina)
9/10/81
1300
Trawl
Oungeness Crab
f
159
540.6?
100.36
-
-
ic05d
MvltfboS Udltil'iMy
9/10/81
1430
Trawl
Dungeness Crab
f
118
22.3.05
49.99
Eyed and blind sides with
embedded bloodwoms.
Eyed and blind sides with
embedded bloodworms
Eyed and blind sides with
embedded bloodworms
Blind side with red-gold
pigmented areas (i-Jma diam.)
caudal fin-reddish. Liver red
Liver yellowisn/broxn^pale)
Blind side with soal' (•; lma)
red flecks toward posterior
(raw appearance) Liver yellowish
with red/brown splotcnes.
Liver yel loutsh/bcige. itcmach
with wfiite/yellow noojles un
eyrior l-fe|| ,
l ip of blind side peciou!
fin missinil.
Liver yellowish with pink
splotches.
Appearance
-------
Tiblt 0-1.coal. Col lie t ion «d Biologic*) bu for fish and Crab Speciaens Taken in Co—enceaent and Discovery Bays.
S.P.A.
laQ. tto.
Station
c
o
O u
at
* —
a ¦?
c
o
0 u
«
1 =
¦¦¦ o
1— (J
c
V O
o —
-o u
o *
5 =
* 3
Species
Sex
Fork
Length
(-0
Tola!
Weight
(9)
lotal
Fillet
Ueight
(9)
Liver Eslinaird
Ueight Age
U) (yrs)
Reaarks
36069
Hylebos Waterway
9/io/m
1430
Irani
Dungeness Crab
F
140
376.45
59.01
•
-
Appearance good. Frozen before
processing.
33020
City Waterway
(Totea Marina)
9/23/B1
1100
Trawl
Dungenesv. Crab
H
178
778.50
-
-
-
Appearance good. Saall'scar* on
left posterior portion of
carapace.
38021
City Katerway
(Iotas Marina)
9/23/81
1100
Trawl
English Sole
M
263
148.70
36.43
2.60
6
Liver dark pink/purple.
38022
City Uatervay
(Totea Marina)
9/23/81
1130
Trawl
Starry Flounder
Platichthys stellatus
H
271
184.46
53.8
2.96
5
Apparent heaorrahaging along
dorsal.ventral and caudal fins
Liver yellowish/rust with purple
1 ns.
3U02J
9/23/81
1130
Irawl
English Sole
M
261
159.90
51.96
3.82
3
City Waterway
(fuw=a Marina)
Liver cream/pink.
38024
City UittfMy
(lolta Harina)
9/23/81
1130
Trawl
English Sole
M
2B1
160.23
47.41
2.10
3
Bloodworms embedded on blind
Sides. Liver cream/pink.
38U25
Ci ty Waterway
(lotea Harina)
9/23/81
1130
Trawl
English Sole
H
289
178.27
48.00
2.98
5
Blind side with red (raw)
tinge.Liver ye1 low/brown.
33026
Citf Waterway
(Totea Harina)
9/23/ei
1130
Trawl
Flathead Sole
lippoqlossoides
F
240
125.80
46.09
2.83
3
Blind side with raw-looking
pink/red splotches. Liver
light creaa brown.
3302;
Point Defiance
Dock
9/23/91
1400
Trawl
English Sole
F
276
160.90
47.43
3.08
6
Soaie erosion of dorsal and
ventral fin nenbranes Liver
brownish purple.
iao2a
Point Oe fiance
Dock
9/23/81
1400
Irawl
Buffalo Sculpin
F
210
235.87
34.76
8.93
3
Liver yellow/orange
10000
44H00
Discover/ Bay
liyltbos Uaterway
10/6/81
11/5/81
1100
1000-
1300
Irani
Hook «
Line
Starry Flounder
'Jhi tespotted Greenliivi
HenuE'raaMos stelleri
F
F
471
331
1455.0
501.36
396.03
147.21
28.48
27.42
11
3
Several saall lesions appearing
reo on blind side pcisibly
associated with copepod
parasites Liver liiilif yellow.
!>tqouck Tufl ot shrinp u.irts
and one snriap. Liver pink
brown with surface white splotch
-es
-------
Table 0-1 cunt. Collection and Biological Data for Fish and Irab Spetiwens Taken in Comenceaent and Discovery Bay*.
© t> Tl -o t! fork Total Fillet Liver Estimated
£ p ^ u i ^ r ^ Length Weight Weight Weiqht Age
lb. iL. Station ^ o £ "o *3 Species Sex (am) (9) (g) (9) (yrs) Remarks
-.4001
Mylebos Uateiway 1
u /s/ai
1U00
1300
Houk T
Line
Uhitespotted G.eenlmg
H
295
292.76
107.33
7.53
1
Liver is light gray with
pink splotcnes.
44002
Mylebos Waterway
11/5/81
1000-
IjOO
Hook ~
ne
Uhitespotted Green1ing
F
246
147.32
40.34
4.49
1
Ventral portion of caudal fin
eroded. Liver purple/oiown
44003
Hylebos Uateiway
U/5/81
1000-
1300
Hook +
Line
Pacific Staghorn
Sculpin
F
274
298.61
56.68
10.49
6
Liver light gray with pink
splotches.
44004
Hylebos Uaterway
11/5/81
1000-
1300
Hook ~
Line
Pacific Staghorn
Sculpin
F
235
173.60
37.37
5. IB
3
Liver light gray with pink
splotches.
44005
Hylebos Uaterway
11/5/81
1000-
1300
Hook ~
Line
Pacific Staghorn
Sculpin
F
239
173.70
39.15
4.92
2
Liver light gray with pink
splotches.
44006
Old Town Duck
U/5/81
1400
Hook *
Line
Rock Sole
It
251
170.59
53.24
2.41
6
Bloodworms embedded on Blind
and eyed sides. Liver gray
with pink tinge.
4601*0
Ci ty Uaterway
(toted Marina)
11/20/81
2030-
2330
Hook *
Line
Pacific Cod
Gadus macrocephalus
F
555
1912
495.91
76.20
3
Liver pale yellow with pink
splotches.
46051
City Waterway
(lotea Marina)
11/20/81
2030-
2330
Hook *
Line
Pacific loacod
H
265
179.65
53.80
7.28
5
Liver light gray with pink
tinge.
46052
City Waterway
(Toten Marina)
11/20/81
2030
2330
Hook *
Line
Pacific Io«cod
H
2o6
153.37
47.10
2.82
5
Liver light gray with pink
purple tinge.
46063
City Uaterway
(lotein Harina)
11/20/81
2030-
23JO
Hook ~
L i lie
Ualleye Pollock
Theraqra chalco^rama
F
394
436.71
105.61
8.61
5
Liver gray with pink tinje.
•i0l)54
11 ty Ua lei way
(lotta flan na)
11/20/81
20J0-
2330
Hook *
Line
Ualleye Pollock
F
490
1
690.22
172.91
19. Ot
6
Liver light gray with pink
tinijc. Several small c-si-iike
structures on liver suWji.2.
-------
Table 0-1. cont. Collection and Biological OiU for Fish and Crab Sped sens Taken In Coaaenceaent and Discovery Says.
t.P.A.
lab. Mo
Sutioa
3
O u
•a
—t •—
* ®
c
o
o u
«*
« —
o
~— ^»
c
t U
3 t
C —
» o
r »..»
Spec i es
Se<
Fork
leng th
(->)
Total
Ueight
(9)
Total
Fillet
Ueight
-------
Table 0-1. coot. Collection and Biologic*! Data for Fish and Crab Specimens Taken in Coonenceaent and Discovery Fa?s.
E.P.A.
Lab. Ho.
SlatlCu
O u
c/
at ~
U o
Species
Fork
Length
Sex (on)
Total
Weight
(9)
Total
fillet
Me 1 yti t
(9)
Liver Estimated
Weight Aye
{9) (vO
Remarks
46002
Ciscuvery Bay
12/1/81
1000
Trawl
tnglish Sole
H
287
209.00
6B.02
4.17
5
•;s()03
Discovery Bay
12/1/ei
1000
Trawl
English Sole
M
265
160.17
46.24
2.32
5
43004
Discovery Bay
12/1/ei
1000
Trawl
English Sole
F
280
176.11
50.7S
2.90
5
48005
biscovery Bay
12/1/81
1000
Trawl
Flathead Sole
F
281
202.37
58.62
3.88
6
48006
Discovery Bay
12/1/81
1030-
1230
Trawl
Dungeness Crab
M
186
807.23
118.70
9.59
-
4300;
Discovery Bay
12/1/81
1000-
1230
Trawl
Ualleye Pollock
F
280
204.30
52.54
10.82
2
4S00U
Discovery Bay
12
1000-
1230
Trawl
Ua1leye Pol lock
H
360
303.67
54.50
5.34
3
48009
discovery Bay
12/1/ei
1030-
1230
Trawl
Dock Crab
Canter productus
M
ISO
322.34
46.03
5.87
-
481)10
Discovery Bay
12/1/81
1000-
1230
Trawl
Ualleye Pol lock
F
432
509.79
123.66
8.97
5
40011
Discovery Bay
12/1/31
1030-
1230
Trawl
Oungenuss Crab
F
172
823.14
68.77
12.81
-
4801L*
Discovery Bay
12,'1/21
IOUO-
1230
Trawl
Ualleye Pollock
M
445
1028.98
282.45
80.46
7
Blind side with red flecks.
liver pink/beige.
Blind side with red flecks.
Uverpirik/bei'ie.
Blind side witn red Hecks.
Liver, pink/beiye.
Blind side wi*h few red fiecks
and sooe coloration. Liver:
1 i
-------
Table 0-1. cont. Collection And Biological Data tor Fish and Crab Speciaens Taken in Coaaenceaent and Discovery Bays.
I.P.A.
Ut. lio.
Station
° %
t =
o
l~ u
Species
Total
Fork Total Fillet
Length Height Weight
Sex (oa) (g, (g)
Liver fstiaated
Weight Age
(g) (y«)
Remarks
48013
Discovery Bay
12/1/81
1000-
12)0
Trawl
Walleye Pollock
F
437
677.28
180.64
50.37
4
Liver light pink/beige with
colled (wora-like) cysts on
surface.
43014
Discovery Bay
12/1/81
1000-
1230
Trawl
Pacific Staghorn
Sculpin
N
285
390.45
73.83
8.63
3
Liver pink/beige.
43015
Discovery Bay
12/1/81
1000-
1230
Trawl
Pacific Staghorn
Sculpin
F
260
233.45
38.11
4.33
3
L ver pink/beige with scarlet
eiies.
<3016
Discovery Bay
12/1/81
1000-
1230
Trawl
Pacific Staghorn
Sculpin
H
301
446.16
66.48
8.38
2
Liver pink/beige with scarlet
edges and tir.t.
43017
Discovery Bay
12/1/81
1000-
1230
Trawl
Pacific Staghorn
Sculpin
F
313
413.17
63.29
6.98
4
Liver brown/pink.
48018
Discovery Bay
12/1/81
1000-
1230
Trawl
Pacific Staghorn
Sculpin
Pacific Toacod
f
224
150.82
29.60
3.51
2
Liver pink/brown.
43014
Discovery Bay
12/1/81
1000-
1230
Trawl
-
200
72.71
19.18
2.14
3
Liver light gray/pink.
49075
01J Town Dock
12/10/8)
2030-
2330
Hook ~
Line
Walleye Pollock
M
364
392.43
104.72
18.35
3
Liver pink/beige with red
splotches. A few wora-like
cysts on surface.
4907c
Old Town Dock
12/10/81
2030-
2330
Hook ~
line
Walleye Pollock
H
358
407.93
111.67
17.67
2
Liver light creaa with red
splotches. Some worn-like
cysts on surface.
49077
Old Tuwn Dock
12/10/81
2030-
2330
llook ~
Line
Walleye Pollock
H
273
208.37
55.17
13.27
1
Liver pink/light beige.
49U78
Old Town Duck
12/10/81
2030-
2330
Hook ~
Line
Walleye Pollack
H
272
171.58
48.30
5.81
2
Liver pink/beige.
49079
Old To»m Dock
12/10/81
2030-
2330
llook «
Line
Walleye Pollock
f
397
595.49
181.08
30.05
7
Liver pink/beige.
-------
Tab is 0-1. coot. Collection And Biolo-jical Data for Fish and Crab Specimen* Taken in Com»*nc enent and Oiscovery Bays.
cue
o a »- o
e.p.A.
Lib Ho.
Station
O O
5 3
O CI
Of
* -
42080
o
M
J9082
490B3
1*384
4403b
JlOOtl
01001
01002
01U03
J1QO)
Old Town Dock
Old Town bock
Old luwn Dock
Old lovm Duck
JIJ Town Cock
Old lawn Dock
Point Defiance
Wk
lomc Qtrunce
IlOCi.
Point Defiance
(jock
I'o int Oc-iunce
Kjii.t i>trfunc.e
Dock
112/10/1)1
12/10/81
12/10/81
12/10/81
12/10/B1
12/10/81
1/6/82
1/6/82
1/6/H2
l/b/82
1/6/82
2030-
2330
2030-
2330
2030-
2330
2010-
2330
2030-
2330
2030-
2330
2000-
2200
2000-
2200
2000-
2200
2000-
2200
2000-
2200
— 3
Species
Hook ~
Line
Hook ~
L ine
Hook •
Line
Hook
Line
Hook
L Hie
Hook ~
L tne
Hook +
Line
Hook »
Line
Hook
I ine
llook t
Line
Hook ~
line
Total
Fork Total fillet Liver E'-tinutcd
Length Weight Height Weight Aye
Sex {•»} (9) (9) (9) lV')
Renarks
Pacific Cod
H
442
480.31
271.6a
13.91
6
livtr pink/beige.
Pacific Hake
f
453
676.14
176.51
74.03
.
Liver pink/bei9e
Pacific Hake
F
447
521.46
166.30
28.52
-
Liver pink/beige with a few
coiled, worm-lite cysts on
surface.
Pacific Hake
M
404
39B. 40
109.12
20.23
-
Liver pink/beige.
Pacific Hike
F
444
409.97
109.OS
15.63
-
Liver dark beige with pink/
wf.i te splotches.
Pacific Hake
F
42S
503.09
151.29
31.08
-
Liver light pirik/beiye with
coiled worm-like cysts on
surface (uncoiled 2.5uit).
Malleye Pollock
H
JfcS
330.4 7
78. IB
6.37
6
Copepod parasites on gills.
Liver pinl/beiye.
Walleye Pollock
F
342
337.>6
121.45
24.41
4
Liver light pink/beige.
Walleye Pollock
F
313
263.86
85.02
7.39
3
Liver light pink/beige.
Walleye Pollock
H
251
140.41
3a.es
7.55
3
Liver tight pink/beige
Pacific Suborn
jculpin
f
2S1
L'CW. 74
44.52
5.42
3
Liver peach with i-cd s^la'.ch«»s.
-------
Table D-l.cont. Collection and llolijlul DiU for Fish iiul Crib Specimens Taken 1n Conenceaent and Discovery Bays.
° j; ° j; ¦§ j| fork Total Fillet liver Estimated
E-P-A. «< — f ~ £ — Length Height Ueight Weight Age
tab. Wo. Station a ,3 £ o 2 3 Species Sex (na) (g) (9) (g) (yrs) Remarks
01005
Point Defiance
nick
1/7/82
1930-
2130
Hook ~
Line
Ualleye Pol lock
F
402
373.19
82.58
4.09
5
Liver saall, brown/beige with
coiled worn-like cysts on
surface.
01006
Point Defiance
Dock
1/7/82
1930-
2130
Hook ~
Line
Pacific Staghorn
Sculpin
F
264
136.92
43.35
6.67
-
Liver peach with red splotches.
0100/
Point Oefiance
Sock
1/7/82
1930-
2130
Hook ~
Line
Pacific Staghorn
Sculpin
f
277
279.14
57.85
10.23
2
Liver peach with red splotches.
-------
APPENDIX E.
Analytical Methods
-------
ANALYTICAL METHODS
Organic Analysis
Tissue Extraction Procedure
Approximately 30 g of fish muscle tissue Mas placed 1n a 100-mL
fluted V1rt1s extraction flask with acid/neutral (A/N) and pesticide
surrogate spikes and 100 mL of methylene chloride/methanol (2:1)
solution. The tissue was ground for 1 minute with a Brlnkman
Polytron homogenlzer. After filtering through a Whatman #1 filter
using a Buchner funnel pre-r1nsed with methylene chloride
(CH2C12)» the tissue was returned to the V1rt1s flask and the
procedure was repeated twice. The residue was discarded after the
final filtration.
The filtrate was filtered again by gravity through another Whatman #1
filter into a Kuderna-Oanish (KD) concentrator, the solvent volume
was reduced on a steam bath using a Snyder column to approximately 10
mL or until foaming occurred and persisted. The organic concentrate
was transferred to a 1-L separatory funnel containing 500 mL of 2%
Na2S04 solution, whose pH was adjusted to 2. After the contents
were shaken for 1 minute, the CH2CI2 layer was allowed to
separate and then drained Into a second 500-mL separatory funnel.
The extraction of the aqueous portion 1n the large separatory funnel
was repeated twice, with two 50-mL portions of CH2CI2. and the
organic extracts combined 1n the 500-mL separatory funnel. The
organic phase was washed twice with 25-mL portions of 2% Na2S04
solution, pH adjusted to 2. Centr1fugat1on was used, when necessary,
to remove any emulsion. The aqueous portion was discarded.
The CH2CI2 fraction was tranferred to a KD'flask fitted with a
Synder column, and the volume reduced to approximately 5 mL. The
samples that were extracted prior to 3 December 1981 were evaporated
to 4.0 mL with a nitrogen (N2) evaporator and divided 1n half for
A/N and pesticide analysis. Samples extracted after 3 December 1981
were reduced to 5.0 mL; 4.0-mL and 1.0-mL portions were taken for A/N
and pesticide analysis, respectively.
Pesticide Extraction Procedure
Approximately 5 mL of hexane was added to the ampule containing the
pesticide fraction. The ampule was then fitted with a mlcro-Snyder
column and refluxed on a steam bath to remove the CH2CI2- A
Flor1s1l column was prepared, rinsed with petroleum ether, and the
hexane extract was transferred to the top of 1t. Two hundred
milliliters of pentane was passed through the column and collected.
This fraction was used for the analysis of polychlorlnateti butadienes
(PCBD's) and PCB's. Next, 200 mL each of the 6X, 15%, and 50X
diethyl ether/pentane mixtures were passed through the column,
collected and combined. The ether/pentane solvent was replaced by
1so-octane by use of a N2 evaporator, and the volume was reduced to
2.0 mL before Injection.
109
-------
Acid/Neutral (A/N) Extraction Procedure
High molecular weight compounds were removed from the A/N fraction by
gel permeation chromatography (6PC) in the following manner. Two
teflon filters were placed in series (a 10 um filter was placed 1n
front of the 0.2 um filter in a stainless steel filter holder). The
filters and sample container were rinsed thoroughly with CH2CI2
before samples were filtered. After filtering, the filtrate volume
was reduced to 1 mL by evaporation with Ng. It was Injected Into
the GPC, which was equipped with a precolumn of styragel (7 um size
exclusion), and three mlcrostyragel columns connected 1n series from
the largest to the smallest size exclusion (!04A, 500 A, and 100
A). A solution of 5X isopropanol in CH2CI? was used as the
carrier solvent at 2 mL per minute. The first 13 minutes of eluate
was saved for later analysis, 1f desired. The second fraction,
containing the compounds of Interest, was collected from 13 minutes
to 24 minutes, reduced to 0.5 mL by N? evaporation, and analyzed by
gas chromatography/mass spectrometry tGC/MS).
Gas Chromatography/Electron Capture Analysis
For the PCBD analysis, 5 mL of the pentane fraction was injected on a
3X OV-225 column at 40°C isothermally for second column confirmation,
a IX 0V-1/2.4X OV-225 column at 40°C was used. Chromatography was
completed in 15 minutes. For the regular pest1c1de/PCB analysis, the
primary column contained 1.5X SP2250//1.95X SP2401, and the secondary
column consisted of 4X SE-30//6X OV-210. Chromatography was
completed in 30 minutes. The operating temperature for both columns
was 200°C. Each column was glass and 6ft X 2 inn. The GC's were
Tracor, Model 222, equipped with either N163 or h3 electron
capture detectors. Three levels of standards were Injected daily
befcce samples were analyzed. Per cent recoveries of the pesticide
surrogate spike, o, p-'DOE, were calculated, but the pesticide
results were not adjusted by these recoveries.
Gas Chromatographv/Mass Spectrometry Analvs1s--Ac1d/Neutral Fraction
Selected tissue extracts from the A/N extraction procedure were
analyzed by GC/MS using a 30 m DB-5 fused silica capillary column
with spHtless Injection, and temperature programing from 50°C to
360aC at 8aC/m1nute. A Flnnigan 3600 with INCOS data system was used.
110-
-------
Gas Chromatography/Mass Spectrometry Analysis—Volatile Organic
Analysis (VOA)
Every sample was analyzed for VOA compounds. Approximately 1 g of
finely sliced tissue was placed in a 40-mL screw cap vial ano held at
-12"C until ready for analysis. Just prior to purging, 10 mL of
organic-free water containing the Internal standards was added to the
vial containing the thawed sample. The vial was connected to a
Tekmar purge and trap instrument, heated in a 60"C water bath and
sparged onto a trap containing 2/3 Tenax and 1/3 silica gel. The
volatile compounds were desorbed at 180 *C onto a 0.2% CW150Q
stainless steel column (80-100 mesh, Carbopack C) held at 30"C.
After desorption, the GC oven temperature was raised to 60"C and held
for 2 minutes, and then programed at 8'C/minute to 18U*C and held for
19 minutes. The GC/MS used was a Finnlgan 3100D with a Rlber SADR
data system.
-Ill-
-------
Table E-l.
METALS:
antinomy
arsenic
beryU t«is
Ctdniun
chrpmtm
copper
t«ad
mercury
ntcke)
lelentui"
thai 1 i lim
itnc
MlSCSllftHEOUS:
cyrntd*
pheioUcs
PESTICIDES:
ildrfn
dieldrin
chJOrdane
*,4' DDT
M" M)£
4,4' 000
otendnul fan
B- endosulfan
endojulfsn suifate
endrfti
endrln a'dehyde
heptachlor
heptachTor epoxide
«BHC
B- BHC
* BHC
/ SHC (Hndjne)
KB 1242
PC8 1CS4
PC8 t22t
PC8 1232
PCS 124B
PCS 1260
PCB 1016
tc.aphene
EPA PRIORITY POLLUTANTS
B^SE/flfUTR^L CQWCIWPS:
acenaphthene
benildlne
],2 ,i>- trrch lornb(r*zeig
heiachtorobenjcue
ht»ach1ora ethane
bf s{2-chlott>ptHy) l?ther
2-ch!oror*aphthat«ne
I dlchl n T^hcnfcie
1,3-dlclilOiotxnjene
1 t^-dichl o iDbenj&ne
3,J' -dichlarofcemldine
2 ,4-dinltfatoluene
2 ,6-dlnitcotolue"e
l,2-d1ph»i«than»
I J-dfchlirrsp'-ocane
trans-t ,3-dichlorflcr'ODene
cis-1 J-d'Chloroprnpene
ethylbeniene
methylene chloride
chlorontef'ane
&rr*iCTrethaoe
brodtoform
brinijdi th lorweth^ne
fluprotrlchlorowethare
d lih I orijdi f 1 uorwe th^ ne
ehlorcdibrO"r»i'»t>tane
tetrachloroetne-ie
toluene
tr1chiorceth«!«»
vinyl chloride
AC 10 COMPOUNDS:
2.-1.6- trichlnmnhenol
p-ClilOrn-m-cr»if>l
2-cM,v-npt>cnnl
2,4-rt(ChlDrpnht'nn|
2.4-d1fl*etl'>'l uheno I
2-n(trophenil
4-n1 troDhfwl
?,h»nol
:»c" ta c h I orouhe* io I
phennl
-112-
-------
APPENDIX F.
Quality Assurance
-------
QUALITY ASSURANCE
The purpose of quality assurance 1n a laboratory 1s to ensure the
reliability and validity of data generated and help maintain control of
the quality of data. Through the use of quality control (QC) samples and
procedures, a more definite evaluation of the precision and accuracy of a
determination can be obtained. The variations 1n data can then be
attributed to real differences among samples, and trends In the data can
be Identified better.
Organic Parameters
Quality assurance (QA) for the tissue samples was measured through uie
use of surrogate spiking compounds 1n both tissue and quality control
(QC) samples. QC samples comprised 281 of all samples analyzed. Table
F-l suranarlzes the QC work.
Table F-l Quality Control Samples Analyzed
Tissue Samples
Fraction Analyzed Blanks Duplicates Spikes
Base-Neutral/
Acid 49 5 6 3
VOA 92 27 6 6
Pesticide/
CB/PCBO 83 5 8
Acid/Neutral Fraction
The compounds used for surrogate spikes for the A/N fraction were
decaf! uoroblphenyl, 2-fluorophenol, and pentafl uorophenol. Th* average
recovery (X +«H for the surrogate spikes for 63 samples was 101 + 241,
371 ~ 231, and 31 ~ 301, respectively. ~
The average recovery for spiked acid and neutral compounds from three
spiked samples, a total of 50 values, was 571 + 441. 01-n-butylphthalate
and bis (2-ethyl-hexyl) phthalate were presentHn all samples and blanks
at low levels. All data were reported uncorrected. In the six duplicate
samples for the A/N fraction, only one sample had any target compounds
that were detected. Six compounds were detected 1n sample 36050, but
their concentrations were below the limit of quantitation. Only one of
the six compounds was detected 1n the duplicate.
113-
-------
VOA Fraction
The surrogate compounds used 1n the VOA fraction were
2-bromo-l-chloropropane (BCP) and parafluorobromobenzene (PFBB). The
surrogates were added to the water/fish mixture just prior to purging.
The percent recovery for all samples, duplicates, and spikes averaged
103X ~ 8.5* for BCP and 95.IX ~ 10.5X for PFBB. THe VOA spiking
compounds were added to the water/fish mixture just prior to purging.
The average recovery for all spiking compounds 1n six samples, a total of
136 values, was 96.7X + 29*.
Methlyene chloride, benzene, and toluene were always present 1n small
amount 1n the VOA blanks. If the Integrated areas of these compounds 1n
the ssxple and blank wer» similar, then the compound was regarded as not
being present. If the area 1n the samples was at least 5 times the area
1n the blank, the uncorrected concentration for the compound was reported.
In the six replicate samples of the VOA fraction, only two compounds were
detected. Trlchloroethylene was present below the limit of quantitation
1n both 44000 and Its duplicate; methylene chloride was present 1n sample
49083 and Its duplicate at the same concentration.
Pest1c1de/PCB Fraction
The surrogate spiking compound, o,p'-DDE, was used to monitor extraction
efficiency. Unusually large dilutions and high PCS concentrations
Interferred with surrogate recovery computation and gave high results for
six samples. However, the per cent recovery still averaged 98*7% + 24.IX
for 96 samples, duplicates, and 5 blanks. If these six results ar?
deleted, the average per cent recovery 1s 94.5X + 15.3X. The blanks were
clean of target compounds. ~
In the pesticide fraction, only four different target compounds were
detected In eight samples and their duplicates. The results are
summarized 1n Table F-2.
Table F-2. Average Range of Duplicate Values for Compounds Oetected 1n
the Pesticide Fraction
Compound Number of Times Detected Average Range (uo/q. wet wt.)
4,4'-ODD
4,4'-00T
4,4'-DDE
PCB 1254
2
0.002
6
0.002
8
0.002
6
0.027
-114-
-------
DETECTION LIMITS
The limit of detection of a compound Is defined as an a.ialyte signal 3
standard de/iations above the peak to peak noise level of the Instrument
1n the region of the expected analyte peak. Any signal not exceeding
this minimum response would be judged as not indicating the presence of
the analyte. The limit of quantitation 1s defined as the analyte signal
that is 10 standard deviations above the noise level of the instrument.
Between these two limits is a region of detection in which a compound can
be identified, but whose concentration Is too low to allow an accurate
estimate of Its value (1).
The symbol "u" 1s used when the value preceding 1t is the quantitation
limit for the particular substance. It means that the concentration, 1f
any, falls below the detection limit. The symbol "m" indicates that the
concentration of the substance falls between its detection limit and its
quantitation limit. The symbol "<" Is used 1n tables and bar graphs
throughout the appendices, and 1s an artifact of averaging. When
quantifiable values are averaged together with indeterminate "u" or "m"
values the quotient is preceded by the "<" symbol.
The quantitation limits of all the target compounds and metals are listed
1n Table F-3 and F-4. The quantitation limits of the VOA compounds are
generally lower than the A/N compounds because the purge and trap
procedure eliminates many matrix problems.
-115
-------
a-
i
Mdrin 1
Chi or eperylene 300.
(1-HitrosodipncnyUaine 30. Fluorene 5.
N-NUro^odi-n-propyUaiine 500. Pheiun threne 5.
bis(2-Ethyihexyl)phthalate 10. Ditienzo (a ,h)ar.tliracene 240.
Sutyl benzyiph thai ate 20. Indepu (1.2,3~c,d)pyrei>e 200.
Ci-n-butylphthalate 3. Pyrcne 20.
01-n-uctylphthalaie 10.
Ac id Compounds (Ppb)
2,4-Oiwelhylpiifciiol 20. 4,6-Otni tro-o-cresol 250.
2-Hi trophcnol ?0. Pentachloruphenol 40.
4-,1i trophenol 200(1. Phenol 10.
2.4-Dinitropnenol 4100.
-------
Table F-3 cont.
Detection Liaits of Organic Compounds Ainlyied in
Comenceaeot Bay T issue *
Acrolein 200.
Acrylom trile 100.
Benzene 10.
Carton Tetrachloride iO.
Chlorobeniene 10.
1,2-dichloroethane 10.
1.1.1-Triciilorðane lo.
1.1-Dichloroethane 10.
1.1.2-Trichl9roethane 10.
1.1.2,2-Tetrachloroethane 10.
VOA Compounds Inph)
Chloroeinane 10.
Cblorofon* 10.
1.1-Dich'.oroe'hylene 10.
1.2-tr-ns-ricliloroethylene 10.
1,2-Dichloropropane 10.
cii-1,3-Oiclilorcpropene 10.
trans-1,3-Dichloropropene 10.
EthyJbenzene 10.
Methylene Chloride 10.
Hethyt Cliloriae 10.
Methyl Broaide 10
Kromofora 10
Bramodichlomethane 10
Trichlorofluorcuiethane 10
Dibromochloromethane 10
Tetrachloroethylene 10
Toluene 10
Trichloroethylene 10
Vinyl Chloride 10
2-LhloroethylVin>l Ether 10
•wet weight basis
-------
Table F-4.
Detection Limits of Metals In Tissue
Element Detection Limit (ppm-wet weight)
Antimony
.01
Arsenic
.01
Beryllium
.003
Cadmium
.002
Chromium
.005
Cob* 11
.005
Coppr-
.005
Letd
.004
Mercury
.0001
Nickel
.005
Selenium
.01
SI lver
.002
ThilUum
.01
Zinc
.02
-------
1 £ * i
------- |