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BIOLOGICAL SURVEY OF PINKHAM CREEK AND THE
NORTH PLATTE RIVER IN THE VICINITY OF
COWDREY, COLORADO
AUGUST 24,1972
TECHNICAL SUPPORT BRANCH
SURVEILLANCE AND ANALYSIS DIVISION
U, S, ENVIRONMENTAL PROTECTION AGENCY
REGION VIII
NOVEMBER 1972

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BIOLOGICAL SURVEY OF PINKHAM CREEK AND THE
TmTpLATTE RIVER IN THE VICINITY OF
COWDREY, COLORADO
August 21\, 1972
EPA Region VIII LIBRARY
Denver, Colorado
TECHNICAL SUPPORT BRANCH
SURVEILLANCE AND ANALYSIS DIVISION
U, S, ENVIRONMENTAL PROTECTION AGENCY
REGION VIII
November 1972

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TABLE OF CONTENTS
Page No.
I.	Introduction 		1
II.	Methods	1
III.	Discussion	1
IV.	Summary	4
V.	Literature Cited 	 5
VI.	Appendix ..... 	 6

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INTRODUCTION
On August 10, 1972, the Enforcement Division of Region VIII requested a
biological survey of Pinkham Creek and the North Platte River near Cowdrey,
Colorado. The survey was requested to document any change in stream biota that
may have resulted from a reported discharge of suspended solids from the Ozark-
Mahoning Company op June 18, 1972.
Biologists from the Surveillance and Analysis Division conducted a survey
of Pinkham Creek and the North Platte River in the vicinity of Ozark-Mahoning's
discharge on August 2*+, 1972. Station descriptions are listed in Table 1.
METHODS
All quantitative samples were collected with a square foot Surber sampler.
Qualitative samples were collected by hand picking organisms from selected
rocks and debris. All samples were preserved in 10% Formalin and transported
to the Regional Laboratory in Denver, Colorado for processing.
DISCUSSION
The Ozark-Mahoning Company mines and processes fluorspar from an area near
Cowdrey, Colorado. Both mining and process waters are discharged to a series
of lagoons where they are treated and then discharged to Pinkham Creek.
However, according to a memorandum from the Enforcement Division, Region VIII,
the company has a history of highly turbid discharges to Pinkham Creek.
Chemical analyses of samples collected from Pinkham Creek at the time of dis-
charge on June 18, 1972, revealed a total solids content of approximately 1600 mg/1.
1

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downstream from the discharge, compared to 22k mg/l total solids upstream.
Suspended solids that are discharged to a stream can settle out on the
bottom and affect the benthic invertebrate community. Areas in which bottom
organisms live may be blanketed by thick layers of sediment which smother
trapped organisms. Organisms such as stoneflies and mayflies have sensitive
gills that may be damaged by the grinding action of sediments. Burrowing
midges may be reduced in number because of a lack of food in the sediment.
Many free-living organisms also tend to drift out of an area in proportion
to the concentration of suspended solids (Gammon, 1970).
During the biological survey, Pinkham Creek upstream from the Ozark-
Mahoning discharge was clear and low in flow (Station 1, Figure 1). The
five foot wide stream had a bottom composed of gravel and rock. Nineteen
kinds of organisms numbering 60 per square foot were collected from this
station. The benthic community, predominantly stoneflies and mayflies, was
indicative of a clean water environment.
Downstream from the discharge (Station 2, Figure 1) the benthic commu-
nity was slightly reduced in number of kinds and total number of organisms
per square foot (11 and 52 respectfully), but did not reflect any damage
that might have occurred from a sediment discharge. Pollution sensitive
stoneflies and mayflies comprised a majority of the benthic community which
appeared to be similar to the clean water community at Station 1 (Table 2).
A thin silt overlay covered a stream bottom of rock and gravel. The only
indication of possible pollution occurring at Station 2 was a black ring or
band around most of the rocks on the stream bottom.
2

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N
Figure I. Station locations for the biological survey of Pinkham Creek
and the North Platte River, Colorado (8/24/72).

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In the North Platte River, upstream from its confluence with Pinkham
Creek, the benthic community was composed of twenty-two kinds numbering 407
per square foot (Station 3, Table 2). The predominant organisms were
caddisflies (370 per square foot), with mayflies and stoneflies also present.
The benthic community was indicative of a slightly enriched but clean water
stream.
Downstream from the confluence of Pinkham Creek with the North Platte
River (Station 4), eighteen different kinds of organisms were collected.
The total number of organisms present was 57 per square foot. The number of
kinds at Station 4 was similar to the clean water communities at Station 1
on Pinkham Creek and Station 3 on the North Platte River (Table 2). The
smaller total number of organisms per square foot collected downstream of
the confluence was due to a reduction in the number of caddisflies from 370
upstream to 38 per square foot downstream (Table 2). Two genera of caddis-
flies were predominant at each location and both genera usually build cases
of loosely made gravel or detritis attached to rocks on the bottom. A
thick layer of sediment would smother these organisms. Because of their
usual sedentary existence, caddisflies would require a longer period of
time to re-inhabit an area than other more mobile organisms. Thus, at Station
4, the area improved from a polluted to a clean water station, but the benthic
community had not completely recovered.
4

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SUMMARY
On June 18, 1972, the Ozark-Mahoning Company was reported to have made a
waste discharge of suspended solids to Pinkham Creek, tributary to the North
Platte River near Cowdrey, Colorado.
A biological survey was conducted on Pinkham Creek and the North Platte
River to determine any change in stream biota that may have resulted from the
waste discharge. At the time of the survey the benthic invertebrate community
of Pinkham Creek was typical of a clean water environment both up and down-
stream from the Ozark-Mahoning discharge. The North Platte River contained a
wide variety of pollution sensitive organisms with only a reduction in
numbers per square foot of caddisflies downstream from Pinkham Creek to indi-
cate that a source of pollution may have affected the river within the past
few months.
5

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APPENDIX

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Table 1. Station Descriptions
Station 1 Pinkham Creek approximately 100' upstream from the Ozark-
Mahoning discharge.
Station 2 Pinkham Creek 30 feet downstream from the State Highway
125 crossing.
Station 3 North Platte River approximately 50 feet upstream from
the confluence with Pinkham Creek.
Station h North Platte River approximately 100 yards downstream
from the confluence of Pinkham Creek.

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Table 2. Bottom Organisms, Pinkham Creek and
North Platte River, Colorado, August 2k, 1972
(Number Per Square Foot)
Station
Pinkham Creek
Ups of Oz.Mah
Pinkham Creek
DWS Oz. Mah @
Hwy 125	
N. Platte
Ups con-
f1uence
N. Platte
DWS con-
fluence
Organi sms
PIecoptera
Isoperla sp.
Nemoura sp.
Pteronarcel1 a sp.
Arcynopteryx sp.
Perli dae
Chloroperlinae
Nemoura
Isogenus
Ephemeroptera
Baet i dae
Baeti s sp.
Ephemerel1 a sp.
Heptaqenia sp.
Paraleptophlebi a
Ameletus sp.
sp.
Heptageni idae
Tri corythodes
Rithrogena sp.
sp.
Tri choptera
Hydropsyche sp.
Aqapetus sp.
Lepidostoma sp.
Psychomyi a sp.
Cheumatopsyche sp.
Brachycentrus
Oeceti s
Coleoptera (Elmidae)
Larvae
Adults
Odonata
Ophioqomphus sp.
Q
Q
k
6
a
27
1
d
1
a
3
5
11
20
3
2
17
a
a
a
3
Q
i
k
203
6
1
158
2
Q
k
2
a
17
2
3
14
1
1
Amphi poda
Gammarus sp.

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Table 2. Continued
Pinkham Creek N. Platte N. Platte
Pinkham Creek DWS Oz. Mah @ Ups con- DWS con-
Station	Ups of Oz.Mah Hwy 125	f 1 uence	f 1 uence
Organi sms
Mol1usca
Physa sp.	Q.
Sphaeridae	Q.
Di ptera
Simulidae	3	2	Q.
Chi ronomidae	Q.	2	3	0.
Tipulidae	8	23
Total Kinds 19 11 22	18
Total Number/Sq. Ft. 60 52 kQ7	57
Q. = Organisms not collected in quantative sample, arbitarily	given value
of 1 for computing number of kinds.

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