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RCRA Corrective Actions Program
RCRA Facility Investigation/ Corrective Measures Study
Ecological Assessment Guidance Document
Draft Interim Final
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This manual was prepared by Dynamac Corporation, Environmental
Sciences Department, for the U.S. Environmental Protection Agency,
Region III, Hazardous Waste Management Division, Office of RCRA
Programs, RCRA Corrective Actions Program through Contract No. 68-
W9-005.
The principal authors of this document were Jon V. Cuizon and
Jennifer M. Hayes of Dynamac. Project Work Assignment Manager at
EPA Region III was Dominique Lueckenhoff. Research and writing
assistants were Steve Sottung and Allison Shields of Dynamac.
The Project Team of this document would like to acknowledge
r Patricia Tan of EPA and Robert E. Stecik, of Dynamac, for their
moral support, planning advice and project development foresight.

Acknowledgement s
(a

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RFI/CMS Ecological Assessment Guidance Document
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TABLE OF CONTENTS
CHAPTER 1 INTRODUCTION
1.	Purpose, Scope and Intent		3
l. 1 Purpose		3
1.2 Ecological Assessment Definition and Objections....	4
2.	Role of Project Ecologist 	.		5
3.	Elements of Ecological Assessment 		9
3.1	Historical Evaluation 		10
3.2	Receptor Characterization..		10
3.3	Hazard Identification		10
3.4	Exposure Assessment		10
3.5	Ecological Risk Characterization 		11
CHAPTER 2 DATA COMPILATION
4.	Historical Evaluation		15
5.	Contaminant (s) of Concern Identification		17
5.1 Selection of Indicator Chemicals		17
CHAPTER 3 FIELD
6.	Site Ecosystems Assessment/
Establishment of Reference Condition 		19
6.1	Qualitative Map of Site Ecosystems 		19
6.2	Sensitive, Protected or Special Status Habitats,
Threatened and Endangered Species			20
6.3	Reference Condition/Baseline Determination 		20
7.	Biological Sampling Plan Objectives 		25
CHAPTER 4 DETERMINATION OF HEALTHY OR IMPACTED ECOSYSTEM
8.	Comparison of Bioparameters to Determine
Healthy or Impacted Ecosystem		27
8.1	Visual Data Comparison		27
8.2	Bioparameter Interpretations: Healthy or Impacted
Ecosystem		28
9.	Level of Impact Determination		29
9.1	Contamination Characterization		29
9.2	Ecological Risk Characterization		29
10.	Determination of Impact Source	 30
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RFI/CMS Ecological Assessment Guidance Document
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CHAPTER 5 CORRECTIVE ACTION ASSESSMENT
11.	Corrective Action		31
11.1	Purpose and Determination		31
11.2	Artificial Wetlands Consideration		32
11.3	In-Situ Bioremediation		32
12.	Monitoring			33
12.1 Monitoring Program Scope and Objectives		33
12.1.1 Monitoring Schedule		33
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RFI/CMS Ecological Assessment Guidance Document
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FIGURES
Figure 1. Project Ecologist Role Within RCRA
Corrective Action Process		 7
Figure 2. Ecological Assessment Within RCRA Corrective
Action Process: Project Progression
Flowchart	 13
Figure 3. Sample Ecoregion Map	 23
APPENDICES
Appendix A. Ecological Basics Reference Materials		37
Appendix B. Keyword Definitions	 41
Appendix C. Wetlands		45
Appendix D. Sources of Information to Assist
in Determining Wetlands 	 47
Appendix E. Sample Site Reconnaissance Form	 48
Appendix F. Alternative Concentration Limits (ACLs)	 50
Appendix G. Ecological Assessment Checklist	 52
Appendix H. Environmental Protection Law and
Regulation Summary 	 56
Appendix I. References 	 60
iii

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Chapter 1
INTRODUCTION
Historically, the programs under the Comprehensive Environmental
Response, Compensation, and Liability Act (CERCLA) and the
Resource Conservation and Recovery Act (RCRA), have focused
primarily on identification, analysis, and mitigation of
chemicals with adverse human health effects. However, with the
dawning of improved technology and better science, increasing
environmental toxicity information, and greater public interest
in the environment, the U.S. Environmental Protection Agency
(EPA) is placing more emphasis on the importance of reducing
ecological risk, in addition to human health risk.
The EPA, in a recent report, The Nation's Hazardous Waste
Management Program at a Crossroads: The RCRA Implementation
Study. July 1990. determined that the RCRA Program, to date, has
not effectively utilized available data on the ecological impacts
and risks posed by chemical releases to the environment. In the
report, EPA made the following recommendations:
-	Foster and develop the capability to conduct ecological risk
assessments for RCRA facilities.
-	Validate and transfer to RCRA Program staff currently available
biological hazard and risk assessment methods that could be
applied to RCRA issues.
-	Focus research efforts to develop ecological risk assessment
methods that could be applied routinely in the analysis of
complex wastes and effects on biological populations,
communities, and ecosystems.
EPA is currently working to establish policies and guidelines for
addressing ecological impacts at regulated facilities. Various
field methods and protocols exist, but there have been no
directives to consistently implement specific techniques in each
program. It is the intention of this document to integrate
existing information on ecological studies with the RCRA
corrective action process in order to establish a consistent
format of environmental evaluation during the RCRA Facility
Investigation (RFI) and the Corrective Measures Study (CMS).
Biological communities (ecosystems) are visible indicators of
environmental health. The concept of chemical source reduction
in the environment is far more difficult for the public to
comprehend than a recognizably clean river, lake or stream.
Ecological integrity implies environmental health, and as can be
seen from many public health assessments, human health is
directly related to ecological health. Therefore, a
comprehensive environmental compliance program will include the
utilization of ecological impacts data, in addition to the
traditional chemical data collected to evaluate and correct
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RFI/CMS Ecological Assessment Guidance Document
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1. Purpose, Scope and Intent
1.1 Purpose
The purpose of this document is to provide an applied scientific
framework for consistently designing and implementing RCRA Facility
Investigation/Corrective Measures Study (RFI/CMS) Ecological
Assessments that will evaluate pertinent ecological aspects of a
RCRA facility. These ecological aspects include, but are not
limited to:
-	Mapping Ecosystems within or near the site requiring protection,
-	Establishment of a Reference Condition
-	Determination of a Healthy or Impacted Ecosystem
-	Effects of the site's contaminants on Impacted Ecosystem(s), and
-	Effects of Corrective Actions
This document is not intended to be a comprehensive guide; instead
it provides a working format for developing an Ecological
Assessment within the RFI/CMS Process. This document cites,
utilizes and compiles available precedented State and Federal
methods and procedures that are applicable to the Ecological
Assessment Process.
Detailed descriptions of specific field or laboratory methods are
out of this document's scope, instead references are given for
further information.
The authors of this document recommend that project managers
designate and consult with qualified, applied Biologists and field
Ecologists as early as possible in the RFI process and throughout
the Corrective Action Process. Early participation by a project
Biologist or Ecologist will ensure that the proper Work Plan is
developed so that representative data is collected, an appropriate
database is initiated and reviewed, and the subsequent report
reflects the actual or potential impacts on the site ecosystem.
This document is designed to facilitate communication between the
Project Manager, Field Biologists/Ecologists, Engineers and
Geologist/Earth Scientists. Support for designing and evaluating
Ecological Assessments may be available from technical assistance
groups in those EPA Regions that have formed them. In other
Regions, Field Biologists/Field Ecologists may be found on the
staffs of other EPA offices and contractors, or on the staffs of
other Federal agencies. The role of these specialists is discussed
in greater detail in section 2 of this document.
Sources for relevant information are given at the end of
appropriate text. Appendix A is a compilation of references for
information on ecological basics.
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RFI/CMS Ecological Assessment Guidance Document
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1.2 Ecological Assessment Definition and Objectives
Ecological assessment, as discussed in this document, is a
qualitative and/or quantitative investigation of the actual or
potential impacts of a RCRA facility's operation on the ecosystem
or parts of ecosystems within the facility's boundary and
surrounding areas.
The objective of an RFI/CMS Ecological Assessment is to assess both
qualitatively and/or quantitatively, the impact and potential
impact of hazardous waste and non-RCRA waste generated, stored,
treated or disposed of at a RCRA Facility on ecosystems within the
site boundary and adjacent areas.
The RFI/CMS Ecological investigative process can provide valuable
data to determine if an ecosystem has been impacted. And in the
case where ecosystem impacts have been identified, these data can
be utilized for designing and implementing corrective action to
cease detrimental impacts and where possible to restore an impacted
ecosystem back to a healthy condition. The Ecological Assessment
requires a thorough evaluation and interpretation of collective
geographic, physical, chemical and biologic factors at and near a
site to describe the nature and extent of the actual or potential
harm to an ecosystem. Therefore, the Ecological Assessment may
also directly supply data for associated Human Health Risk
Assessment calculations.
Ecological Assessment seeks to determine the nature, magnitude, and
transience or permanence of observed or expected effects. One
critical goal of Ecological Assessment is to reduce the uncertainty
associated with predicting and measuring adverse effects of a
site's contaminants (EPA/540/1-89/001).
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2. Role of Project Biologist/Ecologist
Just as the hydrogeologist or engineer is required to be present
during the scoping sessions, it is imperative that an appropriate
ecological representative be present. Early participation by an
ecologist will ensure that the proper Work Plan is developed so
that representative biotic samples are collected, an appropriate
database is developed and reviewed, and the subsequent report
reflects the actual or potential impacts on the site ecosystem.
Figure 1 illustrates Associated Project Ecologist Activities in the
RCRA Facility Investigation and Corrective Action Process. The
steps described in the figure at times overlap and are not
necessarily performed at the same stage for every site.
The involvement of the Project Ecologist is critical in the
development and review portions of the Work Plan, quality
assurance/quality control measures, sampling programs, data
interpretation, and corrective measure analysis. Because the
objectives of the Ecological Assessment review are unique from
other portions of the facility investigations, it is important that
the Project Ecologist include the proper sampling, analytical and
review considerations that are appropriate for ecological
investigations. The Project Ecologist may also recognize cross-
over tasks in other sampling or characterization programs (ie. soil
sampling, hydrologic characterization, human health risk criteria)
that may be utilized as data for Ecological Assessment Review
purposes.
During the sampling phase of the Ecological Assessment, a Project
Ecologist's presence is required to monitor and oversee certain
portions of the sampling program. This may include quality
assurance and quality control (QA/QC) tasks, such as bioassay or
biotic specimen sampling splits, and review of field sampling
procedures and methods. In the event that unforeseen sampling
obstacles or limitations are encountered in the field, the Project
Ecologist can provide assistance or advice on alternative sampling
methods or schemes. The Project Ecologist will validate that
proper sampling protocols have been followed and that the data
collected represents true biotic conditions at the site.
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Figure 1. ASSOCIATED PROJECT ECOLOGIST ACTIVITIES WITHIN RCRA CORRECTIVE ACTION PROCESS
(i)
Regulatory Agency Conducts RCRA Facility Assessment to:
•	Identify solid waste management units,
•	Collect existing chemical data,
•	Identify chemical releases or suspected releases, and
•	Determine need for further Investigation
(2)
Regulatory Agency Issues Permit and Permit Conditions
Enforcement Order to Facility Operator/Owner to:
• Perform chemical release Investigation and/or
> Implement interim Corrective Measures.
(3)
Operator /Owner Conducts RCRA Facility Investigation to
•	Confirm chemical release, and
•	Characterize nature, extent and rate of chemical migration
(4)
Regulatory Agency Performs RFI Based Health/
Environmental Assessment to Determine Need for:
•	Interim Corrective Measures, or
•	Corrective Measures Study.
(5)
Facility Operator/Owner Conducts Corrective Measures
Study
(1 A)
Visual Site Inspection to:
•	Document existing site ecosystems at and near facility,
•	Identify visibly or potentially Impacted blotlc conditions,
•	Review historical chemical handling practices or Incidences that may Indicate a
notable environmental concern,
•	Determine need for further Investigation.	
Review of:	(2A)
•	RFI Work Plan to determine If potential receptors and contaminant, pathways
will be suitably appraised,
•	Interim Corrective Measure Plans to assess potential ecosystem,
impacts and appraise most appropriate remedial technologies.
•	Develop Blotlc/Ecologlcal Sampling Plan and Field Tasks
(3A)
•	Determine indicator chemicals and develop Project Compound List.
•	Monitor and oversee blotlc sampling program.
(4A)
Perform Ecological Assessment to Determine Impact:
•	Assess Site Ecosystem,
•	Establish Reference Condition or Use Established Ecoreglonal data.
•	Define sensitive, protected or special status habitats; threatened
and endangered species within site ecosystem.
•	Conduct blosurveys.
•	Compare subject site and Reference Condition data.
•	Determine If site ecosystem or parts within ecosystem are Impacted.
•	Develop and review database for ecological data.	
(5A)
CMS to assess potential ecosystem effects and appraise most appropriate
corrective actions
(6)
Regulatory Agency Evaluates Corrective Measures Study
and Recommends Appropriate Corrective Measures.
(6A)
• Evaluate various risk scenarios for possible corrective
action Including no action.
(7)
Facility Operator/Owner Performs Corrective Measures
Implementation.
• Design, Construction, Operations, Monitoring and Maintenance
(7A)
Monitor effects of corrective actions on ecosystem and progress
toward completing objective of returning site ecosystem to healthy state
Provide recommendations for ACL values.

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3. Elements of Ecological Assessment
The design and implementation phases of an RFI/CMS Ecological
Assessment begin at the inception of the RCRA Facility
Investigation. Figure 2. illustrates the project progression of an
Ecological Assessment within RCRA Corrective Action Process.
Each site is unique, and therefore, each site demands specific
attention to its distinctive characteristics. There is no
definitive standard to establish the "completeness" of an
Ecological Assessment, however, the following criteria should be
properly evaluated and incorporated; as deemed necessary by the
Project Ecologist; into each Ecological Assessment:
1)	A visual ecological site inspection during the RCRA Facility
Assessment. [A sample Site Reconnaissance Form can be found in
Appendix E. ]
2)	An historical evaluation of the general site conditions,
chemical handling, storage and incidences, land use, and ecological
conditions.[As described in section 4 of this document.]
3)	An evaluation of relevant chemical data associated with the
facility; including locations, mobility, bioavailability, volumes,
concentrations and toxic properties; and the relationship to
ecosystem stress. [As described in section 9 of this document.]
4)	Review of RCRA Facility Investigation Workplan to determine if
potential receptors and contaminant pathways will be suitably
appraised.
5)	An ecosystem map assimilating information such as topographical
features, hydric conditions, flora and fauna, and visual biotic
stress (ie; stressed vegetation and absent indigenous species) . [As
described in section 6.1 of this document.]
6)	Assessment of site ecosystem [As described in section 6 of this
document.]
7)	Establishment of Reference Condition or other comparative
baseline value. [As described in section 6 of this document.]
8)	A comparison of the site ecosystem parameters to a Reference
Condition (healthy ecosystem) , to determine if site ecosystems have
been adversely impacted by chemical releases. [As described in
Section 8 of this document.]
9)	A determination of the proper corrective measures required for
remediating an impacted ecosystem.
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10) A monitoring program to evaluate the long-term effects of
corrective actions on an ecosystem [As described in Section 12 of
this document.]
The Ecological Assessment Checklist provided in Appendix G of this
document provides a basis for determining the level of detail and
data verification presented in a given Ecological Assessment.
More general components of the Ecological Assessment, which may be
recognized as fundamental elements of risk assessment, are outlined
below.
3.1	Historical Evaluation
The Ecological Assessment begins with an evaluation of the data
obtained from the initial RCRA Facility Assessment and any
available historical data pertaining to the facility. Review of
historical data can help in characterization of the subject
facility and aid in the development of a Work Plan for the RCRA
Facility Investigation.
3.2	Receptor Characterization
The receptor characterization process should identify the ecosystem
component at risk by specifying assessment and measurement
endpoints or potential receptors. These receptors may be aquatic
or terrestrial individuals, populations, biotic communities, or
whole ecosystems.
3.3	Hazard Identification
The ecological assessment requires a hazard identification process
to identify specific toxicological effects on the selected
receptors. This involves the collection, organization and
evaluation of all toxicological data available on the chemicals of
concern and an interpretation of whether the stress condition is
consistent with what is known about the chemical(s).
The hazard identification process evaluates chemical and non-
chemical stresses, the type of ecosystem(s) involved, and the
potential effects of the known hazards on an ecosystem. The hazard
identification would include chemical abundance, chemical
locations, and toxicological profiles of the indicator chemicals or
the designated project compound list.
3.4	Exposure Assessment
The ecological assessment estimates the magnitude and nature of the
exposed population to a chemical. Exposure refers to contact
between the identified receptor and the environmental media (air,
water, soil, food) containing the chemical of concern, or direct
contact with the chemical, which may occur from an accidental
spill.
Exposure assessments for ecological risk can involve a diverse
group of species, habitats, and stresses. For chemical stresses,
the possible fate and transport of the chemicals through the
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environment, needs to be considered, as well as the frequency and
duration of exposures. When extrapolating laboratory toxicity data
to field situations, bioavailability of the chemical (or the extent
to which a chemical can be absorbed, ingested or assimilated by an
organism) needs to be assessed for each site-specific potential
exposure pathway.
3.5 Ecological Risk Characterization
The integration of data derived from the receptor characterization,
hazard assessment, and exposure assessment combine to estimate or
characterize the potential chemical impacts on an ecosystem. The
risk characterization can be presented quantitatively or
qualitatively, depending on the approach used in determining the
various biotic parameters, and the applicability of the approach to
the actual site ecosystem.
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FIGURE 2. ECOLOGICAL ASSESSMENT WITHIN RCRA CORRECTIVE ACTION
PROCESS: PROJECT PROGRESSION FLOWCHART
CHAPTER 2
DATA COMPILATION
HISTORICAL EVALUATION
X
IDENTIFY CONTAMINANTS OF CONCERN
(HAZARD IDENTIFICATION)
CHAPTER 3
FIELD
ASSESS SITE
ECOSYSTEM
ESTABLISH REFERENCE
CONDITION

I
BIOLOGICAL SAMPLING
PLAN
I
CONDUCT BIOSURVEYS OR
USE ESTABLISHED
ECOREGIONAL DATA

CHAPTER 4
DETERMINATION OF HEALTH
OR IMPACTED ECOSYSTEM
COMPARISON OF BIOPARAMETERS TO
DETERMINE HEALTHY OR IMPACTED
ECOSYSTEM	
I
NOT IMPACTED
NO ACTION REQUIRED, CONTINUED
MONITORING RECOMMENDED
IMPACTED
1
DETERMINE LEVEL OF IMPACT
1
DETERMINE IMPACT SOURCE

CHAPTER 5
CORRECTIVE ACTIONS ASSESSMENT
DETERMINE POSSIBLE CORRECTIVE ACTIONS
AND CORRECTIVE ACTION IMPACTS

IMPLEMENT CORRECTIVE ACTIONS
]
MONITOR
]
COMPARE TO REFERENCE CONDITION

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Chapter 2
DATA COMPILATION
4. Historical Evaluation
Historical data research can provide valuable data for facility and
subject site characterization. These data may provide information
on past chemical handling and storage practices, land use
variations over time, possible contaminants and current ecological
conditions.
The federal resources listed below can provide data to be utilized
in the historical data research process.
-	U.S. Geological Survey (USGS) Topographic Maps give information
such as site location in relation to surrounding land contours and
physical features such as valleys, upland areas, surface waters and
structures. The contours may provide surface water runoff and
hydraulic gradient directions.
-	U.S. Fish and Wildlife Service (USFWS) Wetland Inventory Maps can
assist in identifying and characterizing wetlands inside site
boundaries and adjacent areas.
-	Natural Heritage Inventory or Natural Diversity Inventory maps
provide probable locations of special status, threatened, rare, and
endangered species. Certain maps illustrate protected critical
habitats, and propagation.
-	U.S. Department of Agriculture and Soil Conservation Service
(USDA-SCS) maps of hydric conditions reflect groundwater within the
region. Mapped aquifer boundaries and interconnections can outline
potential groundwater concerns.
-	U.S. Department of Agriculture and Soil Conservation Service
(USDA-SCS) Soil Survey Maps present soil types and soil
characterization. The maps may indicate the presence of hydric
soil conditions associated with wetlands conditions.
It is highly recommended that aerial photographs be collected to
illustrate the changes in site operation, function, and
construction over time. Aerial photographs can be obtained from
the USDA-SCS, USGS and other private aerial photograph agencies.
Generally, photographs can and should be obtained for every decade
(usually starting around the 1930s in some areas) leading up to the
date of the assessment.
Soil boring logs, obtained from well installation or soil sampling,
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help to classify on-site soil types. On-site survey results from
portable monitoring equipment readings and visual information may
highlight areas with chemical concern.
Records of past ownership and operations can indicate historical
chemical handling and disposal practices. Facility permits outline
current and past operational and waste management practices at the
site.
Under the Community Right-to-Know mandates, companys are required
to maintain files of Material Safety Data Sheets (MSDSs) for
chemicals stored or utilized at the facility. Facilities are
mandated to file SARA Title III documents which specify chemical
quantity, location and container type stored on-site. Reporting
documents indicate chemical spill response and interim corrective
measures which have taken place.
Hazardous Waste Manifests, which state the chemical name and/or
hazard classification of the facility's chemical waste transported
for disposal are kept on file at the facility for at least three
years.
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5. Contaminant(s) of Concern Identification
(Hazard Identification)
5.1 Selection of Indicator Chemicals
The indicator chemical selection procedure is designed to identify
the highest risk chemicals at a site so that the ecological
assessment is focused on the chemicals of greatest concern.
The following chemical characteristics are important factors in
ranking chemicals in the indicator chemical selection process:
-	quantity of chemical stored, disposed of on-site or released,
-	chemical toxicity,
-	measured chemical concentrations at the site,
-	mobility of chemicals through various exposure pathways,
-	bioavailability of the chemicals to the ecosystem, and
-	potential of the chemical to bioaccumulate.
In developing a project compound list or selection of indicator
chemicals, analyzing a group of chemicals as a package; such as the
Target Compound List (TCL), formerly the Hazardous Substances List
(HSL), the Target Analyte List (TAL), or Priority Pollutant List,
found in 40 CFR Part 302.4 and 40 CFR Part 136 respectively; may
reduce the laboratory analytical costs and required sampling time.
A detailed discussion on selection of indicator chemicals for
public health evaluations can be found in Chapter 3 of Suoerfund
Public Health Evaluation Manual. EPA 540/1-86/060.
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Chapter 3
FIELD
6. Site Ecosystem Assessment
/Establishment of Reference Condition
6.1 Qualitative Map of Site Ecosystems
One of the initial steps in assessing a site's ecosystem is to
identify and map observable flora and fauna communities, and
distinct ecosystem boundaries. A properly completed ecological map
provides a visual illustration of the site ecosystem qualities, an
overview of the subject site ecodynamics, and establishes a working
tool for documentation, data analysis and interpretation, decision
making, field work planning, and other logistically significant
applications.
A site ecosystem map should assimilate information such as:
-	topographical features, including drainage features,
-	surface waters,
-	wetlands,
-	vegetation types, and
-	visual biotic stress
within the site's boundaries or near the site.
The various degrees of sophistication may range from hand-drawn
maps, to maps generated from an interactive database system, such
as a Geographical Information System (GIS). The level of detail
required for an ecosystem map depends on the complexity of the
site, the volume and types of compiled data, and the nature and
extent of the chemical impacts on the site ecosystem. GIS mapping
systems allow for more facile manipulations and comparisons of
compiled data, and rapid illustration renderings.
When coupled with chemical data, structural locations, and other
pertinent information reflecting a potential impact on the site
ecosystem, the map becomes one of the most important aspects for
planning and implementing project progression and making final
determinations about the site ecosystem.
Geographical Information System maps generated from database
information is further discussed in Section 8.1 of this document.
A detailed discussion on qualitative mapping of ecological
communities can be found in section 3.3 of Ecological Assessment of
Hazardous Waste Sites: A Field and Laboratory Reference. EPA 600-3-
89/013.
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6.2	Sensitive, Protected or Special Status Habitats, Threatened
and Endangered Species
An important element in assessing site ecosystems is identification
of sensitive environments, critical habitats and the occurrence of
threatened or endangered species within the ecosystem. The
Endangered Species Act endeavors to conserve ecosystems which
endangered or threatened species inhabit, and to conserve the
species themselves (US EPA 230/10-88/041).
Natural Diversity Inventory programs; multiple index data systems
containing locational and ecological information detailing
occurrences of rare and endangered species, significant biological
communities and geologic features; assist in identifying sensitive,
Protected or Special Status Habitats and, Threatened and Endangered
Species. Each of the 50 states has developed its own inventory
program.	Oftentimes these programs are not completely
comprehensive; due to the limited amount of data available; but the
data they can provide can only enhance the project baseline data of
an assessment, and should be consulted when Ecological Assessments
are conducted.
6.3	Reference Condition/ Baseline Determination
A key step in assessing biological impacts and potential impacts of
contaminants on an ecosystem is the establishment of a reference
condition. Standard experimental controls (established reference
conditions) are rarely available; and for most surface waters,
wetland areas and terrestrial ecosystems, baseline data were not
collected prior to an impact. Therefore, impairment must be
inferred from differences between the impact site and established
reference conditions.
Reference conditions describe the characteristics of areas least
impaired by human activities and are used to define attainable
ecosystem parameters such as maximum diversity, uninterrupted
ecotones, appropriate fecundity-to-diversity ratio and predator-to
-prey ratio. A qualified Field Biologist/Field Ecologist is needed
to identify and establish an appropriate reference condition for
each assessment.
Series of ecoregional reference areas that represent attainable
conditions can be consistently referred to when performing
ecological assessments, thereby, eliminating the need to regularly
repeat data collection and establishing a reference site condition
for each project. Ecoregions are regions of ecological similarity
based on hydrologic, climatic, geologic, or other relevant
geographic variables. Ecoregional reference areas must represent
similar habitat types which are representative of the region.
Figure 3 is a sample ecoregion map. Regional EPA offices should be
contacted to determine if ecoregional database systems are
accessible for a given ecological site.
A detailed discussion on establishing a reference condition, can be
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found in Chapter 5 of Biological Criteria. National Program
Guidance for Surface Waters. EPA/440/5-90/004.
A detailed discussion on the Ecoregional Concept can be found in
section 2.5 of Rapid Bioassessment Protocols for Use in Streams and
Rivers; Benthic Macroinvertebrates and Fish. EPA/440/4-89/001 and
Chapter 5 of Biological Criteria: National Program Guidance for
Surface Waters. EPA/440/5-90-004.
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Figure 3. Sample Ecoregion Map
WETLANDS
WOODED WETLANDS
SUBMERSED AQUATIC VEGETATION
SHELLFISH BED CONCENTRATIONS
; PUBLIC LAND AMD HEC8EA3IDN AREAS
l MUD FLATS
: sea wall
SANDY BKACHKS
| SIGNIFICANT MARINE HABITAT ABBA
| CRITICAL HABITAT ABBA
COASTAL ZONE MANAGEMENT BOUND AST
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7. Biological Sampling Plan Objectives
The purpose of a biological field sampling program is to identify
specific ecological parameters and to collect data to assess the
impacts and potential impacts of a contaminant on an ecosystem.
Bioparameters identified during a biological survey may include:
-	Predator-Prey Ratio,
-	Population Fluctuations,
-	Species Diversity,
-	Species Fecundity-Species Diversity Ratio, and
-	Ecotone Quality.
Prior to the implementation of a biological sampling program, it is
recommended that pre-existing data be utilized to develop a formal
sampling work plan. The sampling plan should be detailed in the
Quality Assurance Project Plan (QAPP) and address specific
objectives of the sampling effort, sampling protocols, schedule,
and sampling logistics.
Benthic macroinvertebrates can be collected for use as water
quality indicators using methods and biotic index systems described
by Hilsenhoff (1982, 1987, 1988). These methods endeavor to
collect benthic organisms which are representative of the benthic
taxonomic communities. The biotic index system relates the various
levels of chemical tolerance to the relative abundance of specific
taxonomic groups. The relative abundance of these specific species
is directly related to the degree of organic pollution.
The following references provide a detailed discussion on
biological sampling concepts, criteria and methods:
Rapid Bioassessment Protocols for Use in Streams and Rivers.
Benthic Macroinvertebrates and Fish.EPA/440/4-89/001.
Biological Criteria: National Program Guidance for Surface Waters.
EPA/440/5-90-004, Ecological Assessments of Hazardous Waste Sites:
A Field and Laboratory Reference. EPA/600/3-89/013, and Procedures
fnr Quantitative Ecological Assessments in Intertidal Environments.
EPA/600/3-78-087.
Cooperation with other environmental consultants in various
disciplines (geology, engineering, health risk assessment) and
coordination of sampling events (biological, physical, chemical)
will decrease crossover sampling efforts, and may yield greater
integrity to the collected data.
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Chapter 4
DETERMINATION OF HEALTHY OR IMPACTED ECOSYSTEM
8. Comparison of Bioparameters
to Determine Healthy or Impacted Ecosystem
8.1 Ecosystem Data Comparison
Observable and quantitative bioparametric comparisons between site
ecosystems and the reference condition may include:
-	Species Diversity,
-	Stressed Vegetation,
-	Vegetative Succession,
-	Relevant Vegetative Abundance,
-	Land Use and Land Characteristics, and
-	Riparian Vegetation.
Ecosystem mapping is an invaluable method for illustrating and
comparing data. Comparison of visible or qualitative information
may be adequate for screening ecological impacts and for certain
subject sites. However, qualitative methods may not reflect low-
dose, chronic, long-term influences of chemical impacts on an
ecosystem that can only be detected as subtle fluctuations or
variations in biotic populations.
Quantitative methods can yield large quantities of data that can be
compared statistically by analysis of variance between data sets
(site ecosystem vs. reference condition) . Quantitative analysis of
data sets, over time, may also reveal subtle alterations in biotic
populations, which may not be obvious from a qualitative review.
Investigative methods can be grouped as qualitative or quantitative
approaches. While qualitative methods cannot be used to develop
standards or to quantify results, they can be very effective as
screening or ranking tools to set priorities (EPA, Nov. 1988).
Due to the copious amounts of field datum points, data needs to be
compiled in a way to facilitate its illustration and evaluation.
A database could be used to compile data including: protected,
endangered, threatened and rare species; areas of special,
protected or sensitive status; and inventories of critical habitats
or priority natural resource areas. Significant hydrological and
geological features of the region may also be included. Such a
database could be an analytical tool to aid in the assessment of
chemical impacts on the environment, especially related to regional
ecosystems, and provide decisive information for ecological-risk
management.
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An interactive database can be incorporated with ecological and
health risk assessment calculations, in order to interpret risk
driven remediation calculations, review various contaminant
concentration impact scenarios, and provide pertinent information
to the corrective measures remediation review process. An example
of an interactive database is GIS, Geographic Information Systems
which provide data entry, storage, manipulation, analysis, and
display capabilities for geographic, environmental, cultural,
statistical, and political data in a common spatial framework. The
data associated with spatial data may include site boundary,
chemical characteristics, field measurements, ecosystem mapping and
stressed vegetation data.
Qualitative data compilation and analysis is an excellent screening
method for determining site eco-conditions. It can be very powerful
if executed correctly and can always be utilized for other aspects
of the project. Quantitative analysis of data usually requires
more intensive planning for sampling logistics, data storage and
data analysis, and data interpretation. Quantitative analysis may
not be necessary for some projects, where qualitative data
comparison would be sufficient. A qualified Biologist should be
consulted for determining the appropriate approach and level of
effort required for each project.
8.2 Bioparameter Interpretations: Healthy or Impacted Ecosystem
A qualified, applied Biologist or Field Ecologist familiar with the
regional ecosystems and local ecological variations should be
consulted for interpretation of the compiled data and comparison of
bioparameter data such as reduction in population size, species
diversity, and predator-prey ratio. An Ecologist/Biologist
knowledgeable about regional and local ecological micro-variations
will be able to conclude from properly executed sampling program
site characterization and data analysis if an ecosystem or
components of an ecosystem are impacted or potentially impacted by
a chemical release.
A detailed discussion on bioparameter interpretations can be found
in sections 3.3.1, 3.3.2, and 3.3.3 of Risk Assessment Guidance for
Superfund Volume II; Environmental Evaluation Manual. EPA/540/1-
89/001 and Biological Criteria: National Program Guidance for
Surface Waters. EPA/440/5-90-004.
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9. Level of Impact Determination
9.1	Contamination Characterization
As part of the hazard assessment, a contamination characterization
or analysis of potential or known chemicals at the facility is
required. Determination of the nature and extent of contamination
is comprised of a summary of contamination sources and descriptions
of contaminated areas. The spatial extent of contamination is
determined through review of historical data, sampling and
monitoring of the various media (water, sediments, soils, air).
Contamination characterization is a dual objective with site
characterization incorporating collection of physical and chemical
data and an evaluation of a chemical's potential to cause
ecological harm.
Sample locations and frequency of sampling can follow a randomized
scheme, systematic approach, biased methods or a combination of the
three techniques. The proper approach must be determined on a
site-by-site basis and must evaluate and employ the most effective
method that reflects the actual nature and extent of chemical
contamination of the subject site.
The chemical sampling, including locations and schedules for
groundwater, soil, surface water, sediment, air, and subsurface
gas contamination as described in the Interim Final RCRA Facility
Investigation (RFI) Guidance Manual. EPA 530/SW-89-031 should be
evaluated from an ecological perspective to see if actual site
conditions are being represented over time. That is, ecological
considerations (ie; time of year, proximity to wetlands, etc.) may
influence the locations and times of these sampling events and
parameters.
9.2	Ecological Risk Characterization
Contaminants can cause changes in ecosystem structure and function.
As contaminants modify the species composition and relative
abundance of populations in a community, the complex patterns of
matter and energy flow within the ecosystem may also change. If
certain key species are reduced or eliminated, this may interrupt
the flow of energy and nutrients to other species not directly
experiencing a toxic effect. If plant life is adversely affected
by a contaminant, the ecosystem as a whole may capture less solar
energy and thus support less animal life. If microbial or
invertebrate populations are disrupted, decomposition of dead
plants and animals may not occur rapidly enough to supply
sufficient mineral nutrients to sustain the plant community.
These ecodynamics can be measured both qualitatively and
quantitatively; qualitatively through observable effects and
quantitatively through population census biomass production and
other methods.
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10. Determination of Impact Source
Source characterization aids in determining possible release
pathways, monitoring procedures and the nature and scope of any
corrective actions forthcoming.
Waste characterization enables the RFI project manager and Project
Ecologist to better evaluate monitoring locations and constituents,
monitoring procedures, release rates, and which unit released which
contaminant (EPA/530/SW/-87-001). Waste characterization is also
essential in determining the nature and scope of any corrective
actions needed.
A detailed discussion on source characterization can be found in
section 2.2.3 of Interim final RCRA Facility Investigation (RFI)
Guidance, EPA/530/SW-89-031, Chapter 7 of Draft RCRA Facility
Investigation (RFI) Guidance: Vol I. EPA/530/SW-87-001, Chapter 3
of Risk Assessment Guidance for Superfund. EPA/540/1-89/001, and
Biological Criteria: Natural Program Guidance for Superfund.
EPA/440/5-90-004.
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Chapter 5
CORRECTIVE ACTION ASSESSMENT
11. Corrective Action
11.1 Purpose and Determination
The 1984 amendments to RCRA greatly expanded EPA's authority to
implement Corrective Action at hazardous waste facilities. The
expanded RCRA Corrective Action Program is a cleanup program for
all hazardous waste facilities, whether operating or closing and is
similar in purpose to EPA's Superfund Program.
The purpose of Corrective Action is to cease detrimental impacts
and where possible return impacted ecosystem to a healthy
condition. The type of Corrective Action depends on the
contaminants of concern and site-specific stressed ecological
parameters. Contaminant changes in ecosystem structure and
function need to be interpreted by the Project Ecologist.
The effect, both beneficial and detrimental, of the corrective
action needs evaluation. Various risk scenarios need to be weighed.
The scenarios should consider but not be limited to:
-	impact of excavation,
-	toxicant release through dredging,
-	potential for siltation,
-	treatment technology (on-site and off site),
-	contaminant mobility, bioavailability and toxicity.
Corrective actions such as excavation of contaminated wetland
sediments would result in high levels of siltation to streams,
potential toxicant release and the destruction of a fragile wetland
habitat . Such a corrective action could cause more damage than
the contaminants toxic effects. Long and short term impacts must
be considered for both corrective action and no action scenarios.
A no action alternative is a viable option when ecological
assessments indicate:
-	the lack of bioavailability due to strong contaminant attenuation
to soil, clay or organic matter,
-	contaminants are readily biodegraded in site matrix,
-	or potential for high photolysis exists.
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11.2	Artificial Wetlands Consideration
Constructed wetlands can be utilized as a cost effective treatment
technology for a variety of project chemicals. Constructing a
wetland, where one did not exist before, reduces the influent water
quality requirements associated with natural wetlands, and ensures
more reliable control over hydraulic conditions and vegetation
management. A discussion on the dynamics, importance and
definitions of naturally occurring wetlands can be found in
Appendix C of this document.
Artificial wetlands can be constructed in most climatic regimes.
Certain wetland species can tolerate winter freezing much better
than aquatic plant systems. Experimental systems have been built
in heavy clay soils and in an abandoned mine-tailing basin
(Cobalt). Topography is an important consideration in the
selection of an appropriate site since grading and excavating
represent a major cost factor. Site selection criteria differs
depending on the type of constructed wetland as well. A detailed
discussion on design criteria can be found in chapter 3 of Design
Manual; Constructed Wetlands and Aquatic Plant Systems for
Municipal Wastewater Treatment. EPA/625/1-88/022.
In wetland conditions, metals such as copper, mercury, chromium,
nickel, lead, zinc and cadmium precipitate from water and adsorb to
organic matter, silt, clay, and sediments. Chemical, physical and
biological interactions within the organic benthic layer of
wetlands are the major mechanisms that account for approximately
90-95% of chelation, complexing, adsorption and biological
assimilation. Plant uptake of metals accounts for less than one
percent (Reed et.al, 1988).
It is recommended that exotic species only be introduced if it is
improbable that viable spores/seeds would spread and germinate onto
other areas. Examples of wetland and water body exotics include;
Purple loosestrife Lythrum salicaria. Hydrilla verticillata. Ecreria
(Elodea) densa (giant waterweed).
11.3	In-situ Bioremediation
In-situ bioremediation is a proven technology that cost-effectively
treats organic contamination in subsurface and surface
environments. Bioremediation reduces both contamination dissolved
in ground water and residual soil-bound contamination. To maximize
effectiveness, the technology is implemented after site-specific
laboratory studies are conducted.
Implementation involves enhancing the natural degradation process
by infiltrating necessary nutrients to the contaminated
subsurface/surface, aeration and water recovery. After the process
is complete, the microorganisms die back to prior natural
population levels. Bioremediation is low impact as compared to
excavation or incineration. Another advantage is that it is a
final treatment process; an endpoint is reached.
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12. Monitoring
12.1 Monitoring Program Scope and Objectives
The objective of the monitoring program is to assess the progress
and effectiveness of the corrective action at reaching its
objective of returning the ecosystem to a healthy condition.
Design and implementation of a biological monitoring program is
necessary to effectively evaluate the success of corrective action.
Site specific ecological parameters indicative of ecosystem stress
are selected and monitored. The monitoring program includes
detailed ecological surveys, measurements of the structure and
function of the particular ecoregion, and comparisons to previously
determined reference conditions. The monitoring program's design
needs to address but not be limited to the following:
Time frame and stability of site recovery
Predator/Prey ratio
Appropriate fecundity in relation to species diversity
Maximum species diversity
Uninterrupted ecotones
Duration and season of initial sampling data
Length of monitoring program, ideal and feasible
Analysis of contaminant concentration over time
Aerial photography applications
The benefits of monitoring a RCRA site ecosystem that has been
determined to be a non-impacted site include the potential to
identify concerns that may have gone unnoticed during the initial
phase of EA and to detect new concerns as they occur
12.1.1 Monitoring Schedule
The monitoring program schedule is site-specific. Considerations
must be made as to chemical contaminant half-life,
bioconcentration, biomagnification, biodegradation, photolysis
rate, bioavailability and mobility. The corrective action schedule
must also be considered, for example, corrective action could take
up to 20 years. Monitoring may be necessary during the action if
it is a bioremediation that is slated for 2-5 years and directly
after plus extended monitoring after an excavation remediation.
The duration of monitoring is effected by site size, bioparameters,
contamination characteristics, etc. Data collected during a
corrective action could be used to determine if process
modifications are necessary to increase effectiveness, thereby
taking into account site specific complexities.
Points to consider when designating monitoring locations are
contaminant point of discharge, location of initial data collection
and areas most adversely affected by contaminant discharge.
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Further information can be found in Risk Assessment at Hazardous
Waste Sites (EDs) FA Long, G.Schweitzer ACS. 1982.page 7
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APPENDICES
Appendix A.
Appendix B.
Appendix C.
Appendix D.
Appendix E.
Appendix F.
Appendix G.
Appendix H.
Appendix I.
Ecological Basics Reference Materials
Keyword Definitions
Wetlands
Sources of Information to Assist in Determining
Wetlands
Sample Site Reconnaissance Form
Alternative Concentration Limits (ACLs)
Ecological Assessment Checklist
Environmental Protection Law and Regulation Summary
References
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APPENDIX A. ECOLOGICAL BASICS REFERENCE MATERIALS
Title: Risk Assessment Guidance for Superfund Volume II Evaluation Manual
EPA/540/1-89/001
Key Sections
Chap.3 Basic Concepts for Ecological Assessment
Chap.4 The Role of Technical Specialists in Ecological Assessment
Chap.5 Planning an Ecological Assessment
Title: Biological Criteria. National Program Guidance for Surface Waters
EPA/440/5-90/004
Key Sections
Chap.3 The Conceptual Framework
Chap.5 The Reference Condition
Chap.6 The Biological Survey
App.A Common Questions and Their Answers
Title: Review of Ecological Risk Assessment Methods EPA/230/10-88/041
Key Sections
Chap.2 Discussion and Characterization of Methods
App.C Ecological Assessments Method Summaries
Title: Rapid BioasseBsment Protocols for Use in Streams and Rivers. Benthic
Macroinvertebrates and Fish EPA/440/4-89/001
Key Sections
Chap.2 The Concept of Biomonitoring (Ecoregion Concept)
Chap.3 Overview of Protocols & Summary of Components
Chap.5 Habitat Assessment & Physicochemical Parameters
Chap.6 Benthic Macroinvertebrate Biosurvey & Data Analysis
Chap.7 Fish Biosurvey & Data Analysis
App .A Guidance for Use of Field & Lab Data Sheets
Title: Procedures for Quantitative Ecological Assessments in Intertidal
Envi ronment s EPA/600/3-78/087
Key Sections
Sec. 2 Sampling Program Design Considerations
Sec. 3 Collecting and Processing Samples
Title: Macroinvertebrate Field T.«Vioratorv Methods for Evaluating the
Biological Integrity of Surface Waters EPA/600/4-90/030
Key Sections
Chap.4 Selection of Sampling Locations
Chap.5 Sampling Methods
Chap.7 Data Evaluation
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ECOLOGICAL BASICS REFERENCE MATERIALS (Continued)
Title: Ecological Assessments of Hazardous Waste Sites: A Field and Laboratory
Reference EPA/600/3-89/013
Key Sections
Chap.3 Assessments Strategies and Approaches
Chap.7 Biomarkers
Chap.8 Field Assessments
Title: Protocol for Bioassessments for Hazardous Waste Sites EPA/600/2-83/054
Key Sections
pg.7 Bioassessment Protocol
Title: Superfund Exposure Assessment- Manual
EPA/540/1-88/001
Key Sections
Chap.2 Contaminant Release Analysis
Chap.3 Contaminant Fate Analysis
Title: Methods for Evaluating Stream. Riparian and Biotic Control US
Department of Agriculture
Key Sections
pg.2 Sampling Design
pg.7 Stream Habitat Evaluation
pg.24 Riparian Zone
pg.26 Fish Population Evaluation
pg.36 Macroinvertebrate Analysis
Title: Ecological Risk Assessment Methods: A Review and Evaluation of Past
Practices in the Superfund and RCRA Programs EPA/230/3-89/044
Key Sections
Chap.3 Site-Specific Methods Used to Characterize Actual Impacts at OSWER
Sites
Chap.4 Site-Specific Methods Used to Characterize Potential Impacts at OSWER
Sites
App. A Summary of Site-Specific Methods Used to Characterize Actual Ecological
Impacts
App. B Summary of Site-Specific Methods Used to Characterize Potential
Ecological Impacts
Title: Summary Report on Issues in Ecological Risk Assessments EPA/625/3-
91/018
Key Sections
Sec 2.4 Population Modeling in Ecological Risk Assessments
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ECOLOGICAL BASICS REFERENCE MATERIALS (Continued)
Title:Superfund Public Health Evaluat-i Manual
EPA/540/1-86/060
Key Sections
Chap.3 Selection of Indicator Chemicals
Chap.4 Estimation of Exposure Point Concentrations of Indicator Chemicals
Chap.8 Development of Performance Goals and Analysis of Risks for Remedial
Alternatives
Chap.9 Summarizing the Public Health Evaluation
Title:Guidance for Conducting Remedial Investigations and Feasibility Studies
under CERCLA EPA/540/G-89/004
Key Sections
Sec 1.4 The Remedial Investigation/Feasibility Studies Process Under CERCLA
Title: Federal Manual for Identification & Delineation of Jurisdictional
Wetlands MultiAgency, 1989
Key Sections
Part 2 Mandatory Technical Criteria for Wetland Identification
Part 3 Field Indicators and Other Available Information
Part 4 Methods for Identification and Delineation of Wetlands
App. B Examples of Data Sheets
Title: RCRA Orientati"" Manual EPA/530/SW-86/001
Key Sections
Sec. 2 Subtitle D of RCRA-Managing Solid Waste
Sec. 3 Subtitle C of RCRA-Managing Hazardous Waste
Sec. 4 Subtitle I of RCRA-Managing Underground Storage Tanks
Title: Interim Final RCRA Facility Investigations (RFI) Guidance
EPA/530/SW-89/-031
Key Sections
Vol 1 Procedures to follow in developing a work plan
Vol 2,3 Media-specific for characterization of the nature, extent and rate of
contaminant release
Vol 4 Case studies
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APPENDIX B. KEYWORD DEFINITIONS
Assessment Endpoints: Formal expressions of the actual
environmental values that are to be protected. They are
environmental characteristics, which if affected, indicate the
need for corrective action
Bioavailability: The extent to which a chemical can be
absorbed, ingested or assimilated by an organism.
Bioaccumulation: The net uptake of chemicals by organisms
directly from water or through consumption of food containing the
chemicals.
Bioassay: The employment of living organisms to determine the
biological effects of some substance, factor, or condition.
Bioconcentration: The net uptake of chemicals by aquatic
organisms from water.
Biological Assessment: Evaluation of the biological condition of
a ecosystem using biological surveys and other direct
measurements of resident biota.
Biological Criteria: Numerical values or narrative expressions
that describe the reference biological integrity of ecological
communities inhabiting areas of a given designated life use.
Biological Integrity: The condition of the ecological community
inhabiting an unimpaired area of a specified habitat as measured
by community structure and function.
Biomagnification: The net increase in chemicals in organisms at
successively higher trophic levels as a consequence of ingesting
contaminated organisms at lower trophic levels.
Biamass: The total amount of living material, plants and/or
animals, above and below the ground in a particular habitat or
area.
Biome: A major biotic unit consisting of plant and animal
communities having similarities in form and environmental
conditions.
Bioparameters: Key biological variables such as species
diversity, predator: prey ratio, and fecundity rates that
characterize an ecosystem.
Biosurvey: Collecting, processing, and analyzing representative
portions of a resident community to determine the community
structure and function.
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Biota: The fauna and flora of a given area.
Chronic Toxicity: Involves long-term effects of small doses of a
contaminant and their cumulative effects over time. Theses
effects may lead to death of the organism or disruption of such
vital functions as reproduction.
Community: An assemblage of populations of plants, animals,
bacteria, and fungi that live in an environment and interact with
one another forming a distinctive living system with its own
composition, structure, environmental relations, development, and
function.
Corrective Action: An order EPA issues at a facility when there
has been a release of hazardous waste or constituents into the
environments. Corrective action may be required beyond the
facility boundary and can be required regardless of when the
waste was placed at the facility.
Ecological Assessment: A qualitative and/or quantitative
investigation of the actual or potential impacts of a RCRA
facility on the ecosystem or parts of ecosystems within the
facility's boundary and surrounding areas.
Ecosystem: The biotic community and its environment which,
together, function as a system of complementary relationships,
with the transfer and circulation of energy and matter.
Ecotone: Zone where two or more different communities meet and
integrate. This zone of intergradation may be narrow, wide, or
local (ie. a zone between a field and a forest).
Facility: All contiguous land, structures, and improvements on
the land, used for treating, storing, or disposing of hazardous
waste. A facility may consist of several treatment, storage, or
disposal operational units, e.g., one or more landfills, surface
impoundments, or a combination of the above.
Fecundity: Potential capability of an organism to produce viable
offspring.
Impairment: Detrimental effect on the biological integrity of a
ecosystem caused by an impact that prevents attainment of the
designated use.
Measurement Endpoint: A quantitative expression of an observed
or measured effect of the hazard; it is a measurable
environmental characteristic that is related to the valued
characteristic chosen as an assessment endpoint.
Receptor: The entity (e.g., organism, population, community,
ecosystem) that might be adversely affected by contact with or
exposure to a substance of concern.
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Trophic level: Functional classification of organisms in a
community according to feeding relationships; the first trophic
level includes green plants; the second trophic level include
herbivores; and so on.
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APPENDIX C. Wetlands
Importance and sensitivities
Freshwater and marine wetlands perform several critical ecological
functions as a result of their unique characteristics. These
include :
Hydrologic benefits such as flood attenuation and
groundwater discharge;
Water-quality benefits such as the removal and
cycling of sediments, organic materials and nutrients,
and stabilization of banks and shorelines and control
of erosion;
Chemical absorption/filtration benefits exhibited by
aquatic plants' roots and stems in a wastewater
treatment process;
Mitigating effect that plants have on climatic forces
such as wind, sunlight, and temperature; and
Wildlife benefits such as providing habitats and food
sources for fish, shellfish, waterfowl and other
birds, mammals and wildlife.(EPA/540/1-89/001,
EPA/625/1-88/022)
Wetlands have the capability to filter (via precipitation,
adsorption, biodegradation and plant uptake) out silts, metallics
and organic chemicals from influent water. However, the very
nature of this capability lends fragility to the wetland ecosystem.
There is a carrying capacity past which increased chemical loading
produces a detrimental impact and potential irreversible
modifications of the wetland ecosystem.
Definition
The Environmental Protection Agency (EPA), Army Corps of Engineer
(CE), Fish and Wildlife Service (FWS), and Soil Conservation
Service (SCS) are involved with wetland identification and
delineation. Although each of these Federal agencies has formulated
their own definition of "wetland", all are conceptually the same;
they all include hydrology, vegetation, and soils as their basis
for identifying wetlands.
The regulatory definition used for administering Section 404 of The
Clean Water Act is as follows:
Those areas that are inundated or saturated by surface or
groundwater at a frequency and duration sufficient to support,
and that under normal circumstances do support, a prevalence
of vegetation typically adapted for life in saturated soil
conditions. Wetlands generally include swamps, marshes, bogs,
and similar areas.
On-site determinations of wetlands may be made utilizing two
methods or a combination of the two methods: hydric soil assessment
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procedure, and plant community assessment procedure. These methods
require visual identification and classification of soils and
vegetation in order to determine whether the area is a wetland or
non-wetland.
Detailed descriptions of methods for determining and delineating
wetlands can be found in The Federal Manual for Identifying and
Delineating Jurisdictional Wetlands (January, 1989). Currently,
this manual is under review. Significant revisions to several
areas of the manual, including the baseline determination for
wetlands (hydrology, vegetation, and soils), technical validity of
acceptable indicators of wetland hydrology, definition of "growing
season", and the definition of a disturbed wetland and its
delineation procedures are being considered.
For further information on the determination and delineation of
wetlands and their importance and sensitivities, refer to the
Federal Manual for Identifying and Delineating Jurisdictional
Wetlands (January. 1989), America's Wetlands: Our Vital Link
Between Land and Water (February, 1988), and Mid-Atlantic Wetland:
A Disappearing Natural Treasure (June, 1987).
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APPENDIX D
SOURCES OF INFORMATION TO ASSIST IN DETERMINING WETLANDS
DATA NAME
SOURCE
Topographic Maps (mostly 1:24,000; 1:63,350 for Alaska)
U.S. Geological Survey (Call 1-800-USA-MAPS)
National Wetlands Inventory Maps (mostly 1:24,000;
1:63,350 for Alaska)
U.S. Fish and Wildlife Service (FWS)
(Call 1-800-USA-MAPS)
County Soil Survey Reports
U.S.D.A. Soil Conservation Service (SCS) District
Offices (Unpublished reports-local district offices)
National Hydric Soils List
SCS National Office
State Hydric Soils List
SCS State Offices
County Hydric Soil Map Unit List
SCS District Offices
National Insurance Agency Flood Maps
Federal Emergency Management Agency
Local Wetlands Maps
State and local agencies
Land Use and Land Cover Maps
USGS (1-800-USA-MAPS)
Aerial Photographs
Various sources - USGS, U.S.D.A. Agricultural Stabilization
and Conservation Service, other Federal and State agencies,
private sources
Satellite Imagery
EOSAT Corporation, SPOT Corporation and others
National List of Plant Species That Occur in Wetlands
(Stock No. 024-010-00682-0)
Government Printing office, Superintendent of Documents,
Washington, D.C. 20402
Regional Lists of Plants that Occur in Wetlands
National Technical Information Service, 5285 Port Royal
Head, Springfield, VA 22161 (703-487-4650)
National Wetland Plant Database
FWS
Stream Gauge Data
CE of Engineers District Offices and USGS
Soil Drainage Guides
SCS District Offices
Environmental Impact Statements and Assessments
Various Federal and State agencies
Published Reports
Federal and State agencies, universities, and others
Local Expertise
Universities, consultants, and others
Site-specific Plans and Engineering Designs
Private developers
Table excerpted from the Federal Interagency Committee for Wetland Delineation. 1989. Federal Manual for Identifying and Delineating Jurisdictional Wetlanda. U.S. Army Coipn of Engineers, U.S. Environmental Protection Agency, U.S. FUh and Wildlife Service, anl U.S.D.A. Soil
Conservation Service, Washington, D.C. Cooperative technical publication. 76 pp. plus appendices.

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APPENDIX E. sample SITE RECONNAISSANCE FORM
This form is intended as a preliminary ecological evaluation of
the site. It is a general assessment to characterize the overall
site conditions. This form is to be completed while conducting a
thorough site-walk. All applicable observations (i.e. streams,
ponds, stained soil, possible wetlands, etc.) will be recorded on
a site map. Adjacent properties will also be observed and
documented.
Attach all photographs (labelled), maps and any other pertinent
material that may aid in characterizing the site ecosystems.
The following materials are recommended for field investigations:
-Compass,
-Topographic Map,
-Site Maps
- Camera
-Flora and Fauna Field Identification Books
GENERAL SITE INFORMATION:
Date & Time of Reconnaissance: 	__
Facility Name:
Site Location:
Weather Conditions: 	
Season Reconnaissance Performed:	
Name of Field Personnel and Titles	
ON-SITE RECONNAISSANCE: General Site Observations
1. Label and describe general ecological features such as
grasslands, forests or woods, lakes, streams, wetlands, lagoons
or inundated areas. Whenever possible, describe the general
types of vegetation (woody plants, vines, shrubs, grasses,
emergent, herbaceous, etc.), trees (saplings, mature, deciduous,
coniferous, etc.) and relative abundance (ie; dense, mixed.
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sparse).
2.	Label any visible dead, dying, damaged, or dwarfed vegetation.
3.	Describe the appearance of any observable stream, lake or
ponded water, (clear, color, turbid, etc.)
4.	If possible, determine the approximate depth of the labeled
water body.
5.	If possible, determine the approximate width or width ranges
of the labeled water body.
6.	Describe the flow rates of streams, (stagnant, slow, rapid,
turbulent)
7.	Label and describe any visible drainage into the streams,
including any surface drainage, swales, culverts, springs or
seeps.
8.	Is the riparian vegetation (overhanging vegetation along
stream banks) intact along the streams.
9.	Briefly describe any additional observations relating to
adjacent properties (i.e. lakes, streams, vegetation, etc.).
Person responsible for Site Reconnaissance form (Print name):
Signature:
Date:
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APPENDIX F. Alternate Concentration Limits (ACLs)
Hazardous waste regulations under RCRA require facility owners
and operators to utilize design features and control measures
that protect ground water from hazardous chemical releases.
Under 40 CFR Part 264, Subpart F, all operable units are subject
to groundwater monitoring and corrective measures.
Maximum contaminant limits for groundwater protection are listed
for 14 hazardous constituents under 40 CFR Part 264.94. If the
constituent is not listed, then the concentration limit or
protection standard is set at background water quality level.
Under 264.93, the Regional Administrator will specify, for each
hazardous constituent in the facility permit, concentration
limits or groundwater protection standards for the ground water.
Specifically the concentration of a hazardous constituent:
1)	Must not exceed the background level of that constituent in
the groundwater,
2)	Must not exceed the maximum concentration of the values given
in Subpart 264. 94, and
3)	Must not exceed an alternate concentration limit (ACL)
established by the Regional Administrator.
Variances to the first two protection standards above can be
applied for by the applicant if it can be proven that the
detected hazardous constituents do not pose a substantial present
or potential to human health or the environment. That is the
applicant would apply for an ACL. Some States prohibit the
release of any contaminants to the groundwater and do not allow
for an ACL.
However, in the event that an ACL variance is applicable and
field demonstration is required, an environmental risk assessment
must be performed. The environmental risk assessment must
determine the probable exposure pathways for hazardous
constituents to reach environmental receptors. These exposure
pathways must include characterization of probable surface water
and terrestrial receptors.
If environmental receptors are actually being exposed to ACL
constituents above chronic toxicity levels, or above background
levels if chronic toxicity data are not available, then field
evaluations of the impacts should be performed to support the
proposed ACLs.
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The ACL human health and environmental assessment process is
similar to an ecological assessment in that it utilizes the same
components of risk characterization. And as in an ecological
assessment, an Environmental Biologist or Ecologist should be
consulted for guidance through this process, as specific impacts
to aquatic and terrestrial receptors must be addressed.
More complete information regarding ACLs can be found in
Alternate Concentration Limit Guidance. ACL Policy and
Information Requirements. 1987 EPA/530-SW-87-017.
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APPENDIX 6. Ecological Assessment Checklist
This checklist is intended to assist in evaluation of the extent
to which an RFI/CMS Ecological Assessment was performed.
GENERAL SITE INFORMATION:
RCRA Facility Name 	
Site Location 		
Date of Ecological Assessment (EA) 	
Company that performed EA 	
1. Historical Data Research
Check which of the following elements were included in the
research effort:
	 U.S. Geological Survey (USGS) Topographic Maps
	 U.S. Fish and Wildlife Service (USFWS) Wetland Inventory
Maps
	 Natural Heritage Inventory or Natural Diversity Inventory
Maps
	 U.S. Department of Agriculture and Soil Conservation
Service (USDA-SCS) maps of hydric conditions
	 U.S. Department of Agriculture and Soil Conservation Service
(USDA-SCS) Soil Survey Maps
	 Soil boring logs, obtained from well installation or soil
sampling
	 On-site survey results from portable monitoring equipment
readings
	 Records of past ownership and operations
Facility permits
	 Material Safety Data Sheets (MSDSs)
	 Hazardous Waste Manifests
Were aerial photographs secured for this property?
If so, list the resource agency and dates of the aerial
photographs.		
Was any significant information obtained from the aerial
photograph review? If so, describe this information and if it
was verified (Use a separate sheet if necessary).
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2. Indicator Chemicals or Project Chemical List determined?
	 YES, 	 NO
3. Ecosystem map provided?
	 YES, 	 NO
The ecosystem map illustrates:
	 fauna communities
	 flora communities
	 topography
	 hydric conditions
	 observable biotic stress
wetlands
Provided on separate map:
Was data verified? 	 YES, 	 NO
If yes, how? 	
Was data field tested? 	 YES, 	 NO
Are photographs included? 	 YES, 	 NO
3. Were site or engineering maps secured and utilized?
	 YES, 	 NO,
If yes, map dates?
4. Reference Condition established: 	 YES, 	 NO
Was data verified? 	 YES, 	 NO
If yes, how? 	
Was data field tested? 	 YES, 	 NO
Date of field test 	
Field test performed by 	
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5. Sensitive, Protected or Special Status Habitats, Threatened
and Endangered Species Assessment and identification.
	 Natural Diversity Inventory information consulted
Method used
6. Biosurveys conducted? 	 YES, 	NO
If yes, explain (Use separate sheet if necessary):
7.	Ecological parameters assessed and data provided?
	 Predator-Prey Ratio,
	 Population Fluctuations,
	 Species Diversity,
	 Species Fecundity-Species Diversity Ratio, and
	 Ecotone Quality.
8.	Determination of Healthy or Impacted Ecosystem's within site
boundaries and adjacent areas.
Ecosystem data compared:
	 Species Diversity,
	 Stressed Vegetation,
	 Vegetative Succession,
	 Relevant Vegetative Abundance,
	 Land Use and Land Characteristics, and
	 Riparian Vegetation.
9. Evaluation of effects of site's contaminants on Impacted
Ecosystem(s) provided? 	 YES, NO
If yes, was data verified? 	 YES, 	 NO,
Was data field tested? 	 YES, 	 NO,
If yes, when and by whom	
10. Effects of corrective action evaluation provided?
	 YES, 	 NO
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APPENDIX H. Environmental Protection Law and Regulation Summary
Protecting the environment is a clear objective of the statutes and
directives administered by EPA. There are legal mandates which
require some consideration of ecological impacts. As previously
mentioned, there are no specific procedures, methods or formal
conditions for conducting ecological assessment. Nevertheless,
ecological assessment is clearly encouraged in all of them. The
following is a summary of relevant environmental protection laws
and regulations.
RCRA, Resource Conservation and Recovery Act
The Resource Conservation and Recovery Act of 1976, as Amended.
RCRA requirements for ground-water protection, surface
impoundments, waste piles, underground storage tanks, and surface
treatment are all considered to be potentially applicable for both
human health and protection of the environment at sites that
contain RCRA-listed or characteristic wastes and where waste
management activities took place after the effective date of the
relevant RCRA Subtitle. The RPM or OSC should consult with the
appropriate Regional RCRA staff to make this determination.
CERCLA, Comprehensive Environmental Response, Compensation and
Liability ACT as amended by the Super fund Amendments and
Reauthorization Act
The Comprehensive Environmental Response, Compensation and
Liability Act, CERCLA, as amended by the Superfund Amendments and
Reauthorization Act of 1986, requires EPA to ensure the protection
of the environment in (1) selection of remedial alternatives and
(2) assessment of the degree of cleanup necessary. Several
sections of CERCLA make reference to protection of health and the
environment as parts of a whole.
The Federal Water Pollution Control Act/Clean Water Act
The Federal Water Pollution Control Act, as Amended. This law,
also known as the Clean Water Act, includes numerous sections that
may pertain to Corrective Actions. Section 404 of the CWA, which
requires protection of wetlands, is of particular importance
evaluation.
TSCA, The Toxic Substances Control Act
The Toxic Substances Control Act of 1976. Section 2601 lb) of the
Toxic Substances Control Act states the policy of the Unites States
that "... adequate data should be developed with respect to the
effect of chemical substances and mixtures on health and the
environment....11
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CAA, The Clean Air Act
The Clean Air Act of 1970, as Amended. Under the CAA, EPA has
established National Ambient Air Quality Standards for key
pollutants. In the development of these standards, the Agency
prepares Air Quality Criteria documents that investigate various
effects of exposure to the subject pollutants, including those that
occur on vegetation.
FIFRA, The Federal Insecticide, Fungicide and Rodenticide Act of
1947
The Federal Insecticide, Fungicide and Rodenticide Act of 1947, as
Amended. FIFRA requires that all pesticides be registered with
EPA. To obtain registration, manufacturers must supply EPA with
certain data concerning environmental fate and transport, health
effects, and ecological effects. EPA's Office of Pesticide
Programs (OPP) has issued Registration Standards, which summarize
the Agency's assessment of many pesticide active ingredients.
Endangered Species Act
Endangered Species Act of 1973, as Reauthorized in 1988. Section
7 of the Act requires Federal agencies to ensure that their actions
will not jeopardize the continued existence of any endangered or
threatened species. The U.S. Fish and Wildlife Service and the
National Marine Fisheries Service have primary responsibility for
this Act.
Fish and Wildlife Conservation Act
Fish and Wildlife Conservation Act of 1980. Section 2903 requires
States to identify significant habitats and develop conservation
plans for these areas.
Marine Protection, Research and Sanctuaries Act
Marine Protection, Research and Sanctuaries Act of 1972. Section
1401 declares the U.S. policy of regulating dumping to "— prevent
or strictly limit the dumping into ocean waters of any material
which would adversely affect human health, welfare, or amenities or
the marine environment, ecological systems, or economic
potentialities."
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Coastal Zone Management Act
Coastal Zone Management Act of 1972. This legislation is designed
to (a) encourage States to develop management plans to protect and
preserve the coastal zone, and (b) ensure that Federal actions are
consistent with these management plans.
Wild and Scenic Rivers Act
Wild and Scenic Rivers Act of 1972. Section 2171 declares that
certain rivers "... possess outstanding remarkable scenic,
recreational, geologic fish and wildlife, historic, cultural, or
other similar value" and should be preserved. If corrective action
is taking place at or near a river, a determination of whether it
has been designated as "wild and scenic," and whether there are any
action-specific ARARs regarding the site or its contaminants is
recommended. The National Park Service has primary responsibility
for this Act.
Fish and Wildlife Coordination Act
Fish and Wildlife Coordination Act, as Amended in 1965. Section
662(a) states that the Fish and Wildlife Service must be consulted
when bodies of water are diverted or modified by another Federal
Agency. The facility is to be constructed "with a view to the
conservation of wildlife resources by prevention of loss, or damage
to such resources as well as providing for the development and
improvement thereof..."
The Migratory Bird Treaty Act
The Migratory Bird Treaty Act of 1972 implements many treaties
involving migratory birds. This statute protects almost all
species of native birds in the U.S. from unregulated "take", which
can include poisoning at hazardous waste sites. The act is a
primary tool of the U.S. Fish and Wildlife Service and other
Federal agencies in managing migratory birds.
The Marine Mammal Protection Act
The Marine Mammal Protection Act of 1972. This law protects all
marine mammals, some but not all of which are endangered species.
The National Oceanic and Atmospheric Administration has primary
responsibility for this Act. The Fish and Wildlife Service also
has responsibility for some species.
Excerpted from Risk Assessment Guidance for Superfund. Volume II:
Environmental Evaluation Manual. Interim Final. EPA/540/1-89/001.
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APPENDIX I. REFERENCES
Federal Interagency Committee for Wetland Delineation. (1989).
Federal Manual for Identifying and Delineating Jurisdictional
Wetlands. U.S. Army Corps of Engineers, U.S. Environmental
Protection Agency, U.S. Fish and Wildlife Service, and USDA
Soil Conservation Service. Washington, DC. Cooperative
technical publication. 76 pp. plus appendices.
Hilsenhoff, W.L. 1982. Using a Biotic Index to Evaluate Water
Quality in Streams. Technical Bulletin No. 132. Department
of Natural Resources, Madison, Wisconsin.
Hilsenhoff, W.L. 1987. An improved biotic index of organic stream
pollution. Great Lakes Entomologist 20:31-39.
Hilsenhoff, W.L. 1988. Rapid field assessment of organic pollution
with a family-level biotic index. J.N. Am. Benthol. Soc.
7(1):65-68.
Planning Research Corporation. (1985).	The Endangerment
Assessment Handbook. Office of Waste Programs Enforcement.
Washington, DC.
Tetra Tech. (1987). Background Information Document for
Floodplains. Waste Management Division. Office of Solid
Waste. US Environmental Protection Agency.
Tetra Tech. (1987). Background Information Document for Surface
Water. Waste Management Division. Office of Solid Waste. US
Environmental Protection Agency.
Tetra Tech. (1987). Background Information Document for
Wetlands. Waste Management Division. Office of Solid Waste.
US Environmental Protection Agency.
U.S. Environmental Protection Agency. (1978). Procedures for
Quantitative Ecological Assessments in Intertidal
Environments. Environmental Research Laboratory. Corvallis,
OR. EPA/600/3-78/087.
U.S. Environmental Protection Agency. (1983). _ Protocol for
Bioassessment of Hazardous Waste Sites. Office of Research
and Development. Corvallis, OR. EPA/600/2-83/054.
U.S. Environmental Protection Agency. (1986). RCRA Orientation
Manual. Office of Solid Waste. Washington, DC. EPA/530/SW-
86/001.
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U.S. Environmental Protection Agency. (1986). Superfund Public
Health Evaluation Manual. Office of Emergency Remediation and
Response. Washington, DC. EPA/540/1-86/060.
U.S. Environmental Protection Agency. (1988). America's Wetlands,
Our Vital Link Between Land and Water. Office of Wetland
Protection. EPA/OPA/87/016.
U.S. Environmental Protection Agency. (1988). Constructed
Wetlands and Aquatic Plant Systems for Municipal Wastewater
Treatment. Office of Research and Development. Washington,
DC. EPA/625/1-88/022.
U.S. Environmental Protection Agency. (1988). Guidance for
Conducting Remedial Investigations and Feasibility Studies
Under CERCLA. Office of Emergency Remediation and Response.
Washington, DC. EPA/540/G-89/004.
U.S. Environmental Protection Agency. (1988).	Review of
Ecological Risk Assessment Methods. Office of Policy
Analysis. Office of Policy, Planning, and	Evaluation.
Washington, DC. EPA/230/10-88/041.
U.S. Environmental Protection Agency. (1988). Superfund Exposure
Assessment Manual. Office of Remedial Response. Washington,
DC. EPA/540/1-88/001.
U.S. Environmental Protection Agency. (1989). Ecological
Assessment of Hazardous Waste Sites: A Field and Laboratory
Reference. Environmental Research Laboratory. Corvallis, OR.
EPA/600/3-89/013.
U.S. Environmental Protection Agency. (1989). Ecological Risk
Assessment Methods: A Review and Evaluation of Past Practices
in the Superfund and RCRA Programs. Office of Policy
Analysis. Office of Policy, Planning, and Evaluation.
Washington, DC. EPA/230/3-89/044.
U.S. Environmental Protection Agency. (1989). Rapid Bioassessment
Protocols for Use in Streams and Rivers, Benthic
Macroinvertebrates and Fish. Office of Water. Washington,
DC. EPA/440/4-89/001.
U.S. Environmental Protection Agency. (1989). Risk Assessment
Guidance for Superfund, Volume II Environmental Evaluation
Manual. Office of Emergency Remediation and Response.
Washington, DC. EPA/540/1-89/001.
U.S. Environmental Protection Agency. (1990). Biological
Criteria,National Program Guidance for Surface Waters. Office
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of Water Regulations Standards. Washington, DC. EPA/440/5-
90/004.
U.S. Environmental Protection Agency. (1990). Macroinvertebrate
Field and Laboratory Methods for Evaluating the Biological
Integrity of Surface Waters. Office of Research and
Development. Washington, DC. EPA/600/4-90/030.
U.S. Environmental Protection Agency. (1991). Summary Report on
Issues in Ecological Risk Assessments. Office of Health and
Environmental Assessments. Washington, DC. EPA/625/3-91/018.
Reed, Sherwood, et.al (1988). Natural Systems for Waste Management
and Treatment. McGraw-Hill. Inc.
Tiner, Ralph W. (1987). Mid-Atlantic Wetlands, A Disappearing
Natural Treasure. US Fish and Wildlife Service. 28 pp.
Versar. (1986). Draft Superfund Exposure Assessment Manual.
Office of Emergency Remediation and Response.	US
Environmental Protection Agency. Washington, DC.
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