WATER QUALITY SURVEY
OF THE
HEAD OF THE CHESAPEAKE BAY
MARYLAND TRIBUTARIES
1969
DATA REPORT
Number 12
Chesapeake Technical Support Laboratory-
Middle Atlantic Region
Environmental Protection Agency-
Water Quality Office

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*n>
Chesapeake Technical Support Laboratory
Middle Atlantic Region
Environmental Protection Agency
Water Quality Office
1969 Data Report
Number 12
WATER QUALITY SURVEY
OF THE
HEAD OF THE CHESAPEAKE BAY
MARYLAND TRIBUTARIES
by
James W. Marks
and
Orterio Villa, Jr.
U.S. ICogion Hi
Regional Center for Environments
information
1650 Arch Street (3PM52)
Philadelphia, PA 1910S
Laboratory Staff
Jean Klaczkiewicz, Chemist
Thomas E. Kleczynski, Biologist
Evelyn P. McPherson, Technician
Anna R. Favorite, Statistician
4>.	/O
* PRO"t^
Regional Ccnicr for Environmcnial Information
US PA Rcginn III
1650 Arch Sc
Philadelphia. PA 14103

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TABLE OF CONTENTS
Page
I INTRODUCTION	 1
II STATION LOCATIONS	 6
III SURVEY RESULTS	 7
IV MP					 appendix i

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I INTRODUCTION
A.	Purpose and Scope
During the year of 1969 the Chesapeake Technical Support
Laboratory, Middle Atlantic Region, Environmental Protection
Agency, Water Quality Office sampled the head of the bay
tributaries three times during June, July and September.
Tributaries sampled were Sassafras River, Elk River, Bohemia
River, C and D Canal, Northeast River and two points in the
upper bay. The purpose of this survey was to determine the
levels of eutrophication of the tributaries entering the
Chesapeake Bay.
B.	General Remarks
The monthly surveys included analysis for Dissolved
Oxygen, Chlorophyll a and Phaeopigments as well as nutrients.
C.	Sampling Procedures
Samples were obtained using a small submersible pump
attached to a wire cable calibrated in feet. The pump was
lowered to the desired depth and allowed to run for two minutes
before receiving samples in plastic cubitainers. Dissolved
oxygen (DO) samples were obtained directly from the pump outlet
extending to the bottom of a conventional 300 ml DO bottle.
The sample was allowed to overflow the bottle several times
and then fixed immediately. All samples were immediately

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2
stored on ice and analysis started upon return to the Chesapeake
Technical Support Laboratory, generally within two hours of
sampling.
D. Measured Parameters and Analytical Methods
1.	Water temperature was read from a Beckman Salinometer.
2.	Light extinction, in inches, was made with a 12"
(30 cm) white secchi disk.
3.	The pH measurements were made with a field pH meter.
Conductivity was read from a calibrated Beckman
Salinometer.
$. Dissolved Oxygen
Reference: Standard Methods for the Examination of
Water and Wastewater, 12 ed.. 1965.
Dissolved Oxygen was determined by the azide
modification of the basic Winkler method with the titration
done potentiometrieally with a Fisher automatic "titralyzer."
6. Total Phosphorus
Reference: Menzel, D.W. and Corwin, N. 1965.
The Measurement of Total Phosphorus in Seawater Based on the
liberation of Organically Bound Fractions by Persulfate Oxidation,
jimonology and Oceanography, 10: 280-282.
Murphy, J. and Riley, J=P.. 1962. A Modified Single
iolution Method for the Determination of Phosphate in Natural
raters. Analytica Chimica Acta, 27: 31-36.

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3
Total Phosphorus was determined after persulfate
oxidation of the sample in an autoclave at 15 psi for 30
minutes. The resultant ortho-phosphate was then determined
colorimetrically as the molybdenum-blue complex with the
optical density measured at 882 inn.
7.	Inorganic Phosphorus
Reference: Murphy, J. and Riley, J.P.. 1962. A
Modified Single Solution Method for the Determination of
Phosphate in Natural Waters. Analytica Chimica Acta, 27: 31-36.
Inorganic Phosphorus was determined colorimetrically
as the molybdenum-blue complex with the optical density measured
at 882 nui.
8.	Total Kjeldahl Nitrogen
Reference: Standard Methods for the Examination of
Water and Wastewater, 12 ed.. 1965.
Total Kjeldahl Nitrogen includes ammonia and organic
nitrogen and was determined by the standard micro-kjeldahl
procedure. The sample was digested in the presence of strong
acid to convert the organic nitrogen to ammonia. The ammonia
was then distilled, collected in boric acid solution,
nesslerized and determined colorimetrically.
9.	Nitrate + Nitrite
Reference: A Practical Handbook of Seawater Analysis,
J.D.H. Strickland and T.R. Parsons, Bulletin 167, Fisheries
Research Board of Canada, Ottawa, Canada. 1968.

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h
Nitrate plus nitrite nitrogen was determined by the
cadmium reduction of nitrate to nitrite and subsequent
diazotization -with sulphanilamide and N- (l-napthl )-
ethylenediamine dihydrochloride. The results were calculated
as nitrite nitrogen.
10.	Ammonia
Reference: Southeast Water Laboratory, FWQA,
methodology for the colorimetric determination of ammonia
by the phenol-hypochlorite reaction.
FWPCA Methods for Chemical Analysis of Water and
Wastes, 1969 ed., pp 137lU3-
Ammonia nitrogen was-determined by automation of the
phenol-hypochlorite procedure as described in the Southeast Water
Laboratory Methodology and later adopted as the official FWPCA
procedure. The intensity of the indophenol blue color, formed by
the reaction of ammonia with alkaline phenol hypochlorite, was
increased using sodium nitroprusside as an intensifying agent.
The optical density was measured at 630 and calculated as NH-N.
11.	Chlorophyll a
Reference: A Practical Handbook of Seawater Analysis,
J.D.H. Strickland and T.R. Parsons, Bulletin 167, Fisheries
Research Board of Canada, Ottawa, Canada. 1968.
Chlorophyll a was determined by extraction of millipore-
filtered samples in 90$ acetone and read spectrophotometrically.

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12. Phaeopigments
Reference: A Practical Handbook of Seawater Analysis,
J.D.H. Strickland and T.R. Parsons, Bulletin 167, Fisheries
Research Board of Canada, Ottawa, Canada. 1968.
After Chlorophyll a had been determined the sample
was acidified with one normal HC1. The absorbancy of the
acidified sample was obtained and the percentage of Chlorophyll
present as phaeopigments was calculated.

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6
II STATION LOCATIONS
STATION NUMBER	STATION LOCATION
1	Sassafras River, Georgetown Bridge
2	Sassafras River, Nun 6
3	Sassafras River, Can 3
I4.	Sassafras River, off Betterton
5	Bay, midway between Nun 2 and Bell 1
6	Bay, Turkey Point, Nun 6
7	Elk: River, Nun 10
8	Bohemia River, Nun 2
9	Behemia River, above bridge
10	Elk River, Can 1^
11	C and D Canal, Nun 26
12	Elk River, Nun 6
13	Elk River, Nun ill
111	Northeast River, Nun 2
15	Northeast River, Nun 10'
16	Northeast River, Nun 18
17	Northeast River, mouth of creek

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Ill SURVEY RESULTS
1969
HEAD OF THE CHESAPEAKE BAY TRIBUTARIES
Date
Sample
Taken
Time
Sample
Taken
Sample
Depth
Feet
Secchi
Disc
Inches
Water
Temp.
C
Field
pH
Cond.
jjmhos
DO
mg/l
Total P
mg/l
>4
Pi
mg/1
P04
TKN
mg/l
Organic
N
mg/l
N02+N03
mg/l
nh3
mg/l
Chloro-
phyll a
.K A
Phaei
pigmei





Station
1 - Sassafras
River, Georgetown
Bridee





6-24
1655
Surface
24
27


11.79
.29
ND
.15
 15
< .01
ND
99
28


10

29.5


11.26
.21
ND
.97
 97
< .01
ND


7-15
0900
Surface
18
24.9

 70
8.60
35

1.32
1.30
ND
.02



0845
10

 24.6

.84
8.25
 33

 30
.29
ND
.02


7-21
Surface
12
28.5



 51
ND


ND
ND
97-5
117
9-09
1135
Surface
16
25-5


4.99
^5
03
1.37
.95
 03
.42



10

25


4.23
.40
.006
1.26
.87
03
.40







Station
2 - Sassafras
River, Nun
6






6-24
1630
Surface
46
26


9.03
.12
ND
 59
 55
< .01
.04
27
11


10

27


8.34
.13
ND
.43
.42
< .01
.01


7-15
0920
Surface
27
24.6

.64
9-14
.16

1.40
1.38
ND
.02




10

24.3

.66
8.42
 31*

1.58
1.57
ND
.01


7-21
0908
Surface
18
28



 19



 03
ND
52.50
43
9-09
1115
Surface
24
25


7.40
.26
ND
 79
 78
ND
.01
93-75
53


10

25


6.91
.24
ND
 72
.69
ND
.03







Station
3 - Sassafras
River, Can
3






6-24
1620
Surface
50
26


7-9^
 13
ND
.50
.47
< .01
.02
20.25
23


15

27


7-95
 13
ND
 50
.47
< .01
.04


7-15
0935
Surface
34
24.3


9-75
.12



ND
.01




15

24.1


9.25
.12

.58
.56
ND
.02


7-21
0913
Surface
27
28



15
ND


.04
 03
29.25
^3
9-09
1100
Surface
26
24.5


8.02
.22
.003
 79
 73
.02
.06
60.00
3^


* 15

24


7.89
.23
ND
.74
 72
.02
 03







Station
4 - Sassafras
River, off
Betterton





6-24
1200
Surface
33
25.5


6.73
 19
.01
.40
 32
 30
 07
45.00
20


10

27


6.75
 15
ND
.43
.40
.22
 03


7-15
0955
Surface
38
23.76

1.85
9.28
.14

.60
58
.17
.03




10

23.33

3-5^
7.20
.12

 50
.47
 19
 03

24
7-21
0930
Surface
32
27.5



.11
ND


.04
 05
19.50
9-09
io4o
Surface
26
24.5


7.74
.23
ND
.75
.69
.07
.07
75.00
25


10

24.5


.7.31
.27
.005
.69
.64
.07
.04


ND - Not detectable

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1969
HEAD OF THE CHESAPEAKE BAY TRIBUTARIES
Late	Time	Sample Secchi Water	Total P Pi	Organic NO +N0.. KH_ Chloro-	Phaeo-
Sample Sample Depth	Disc	Temp. Field Cond. DO	g/l mg/l TKN	N	phyll a pigments
Taken	Taken	Feet	Inches	C	pH jumbos mg/l	PO^	PO^ nig/l mg/l	nig/l mg/l	Hg/l	^g/l
Station 5 - Bay, Midway between Nun 2 and Bell 1
6-21+
1210
Surface
22
25

7.26
.11+
.01
.26
.22
.26
 03
16.2


15

26

7.28
 15
.01
 39
.28
.27
.10

7-15
1000
Surface
38
23.8

2.0 9-33
.12

 53
 51
.20
.02



15

23.3

3.4 7.50
.13

 58
 5*+
.20
.01+

7-21
09^5
Surface
30
27.5


.18
ND


.01+
.10
31.5
9-15
101+5
Surface
36
23.5

8.85
.38
.10
.62
.1+7
.16
15
57.00


15

23.5

7.26
.61
 3^
 96
 92
.16
 03






Station 6
- Bay, Turkey Point, Nun
6





6-21+
1220
Surface
22
25

7.21
.18
.001+
.1+1
 35
.27
 07
21+.25


15

25

6.72
.20
ND
.1+1
 37
 27
.01+

7-15
111+0
Surface
30
21+.8

9-55
 39

.1+7
.M+
.20
.02



15

23.9

8.78
.13

 5"+
 52
.20
.02

7-21
1000
Surface
20
28


.26
ND


.07
.08
1+6.50
9-15
1100
Surface
' 21+
23.5

7.17
.28
.02
 70
.67
.21+
 03
60.00


15

23.5

7.21+
.28
.02
 70
.67
.21+
.03






Station 7
- Elk River, Nun
10






6-21+
121+5
Surface
25
25

9-33
 85
.02
.1+8

 50
 03
25-5


15

26.5

7.00
1.00
ND
.1+1
 37
.1+9
.01+

7-15
1150
Surface
21+
21+.5

9.19
.81

-70
.68
.36
.02



15

21+

8.30
.88

.79

.39
.01+

9-16
1015
Surface
18
23.5

7.72
.26
.01+
.80
.7^
 35
 07
82.50


15

23.5

7.67
 36
 03
 83
 78
 36
 05






Station 8
- Bohemia River,
Nun 2






6-21+
1255
Surface
23
25

9-01
.21+
ND
1.37
1-35
< .01
.03
59.25


10

25

8.83
.16
ND
1.1+6
1.1+2
< .01
.01+

7-15
11+50
Surface

27.7

12.81+
 39

2.02
2.00
ND
.02



8

24.7

9-95
.36

1.86
1.81
ND
.05

7-21
1021
Surface
11+
28


.1+6
ND


.08
ND
111.00
9-16
1025
Surface
8
21+

13.1+8
.63
ND
2.70
2.67
.12
.02
315.75


. 10

21+

10.05
 89
ND
2.72
2.60
.12
.13

ND - Not detectable

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1969
HEAD OF THE CHESAPEAKE BAY TRIBUTARIES
Date	Time	Sample Secchi Water	Total P PI	Organic NOg+NO HH	Chloro- Phaeo-
Sample Sample Depth	Disc	Temp. Field Cond. DO	mg/l mg/l TKN	N	phyll a pigments
Taken	Taken	Feet	Inches	C	pH pmhos nig/l	PO^	PO^ mg/l ng/l	^g/l	mg/1	^g/l	^g/l
Station 9 - Bohemia River, above bridge
6-24
1305
Surface
26
26
9-01
.14
ND
1-57
1.56
<
.01
.002
93-00

10

28
8.93
 33
ND
1.27
1.26

.67
ND

7-15
1505
Surface
13
28.1
14.50
 45

1.87
1.85

ND
.01



 10

26.2
12.21
A5

1.99
1.98

.02
.01

7-21
1045
Surface
12
27

 53
ND



.47
50
84.00
9-16
1040
Surface
6
24
10.57
.73
ND
3.04
3.01

.12
 03
387.00


10

24
10.49
 79
ND
3.54
3-54

.12
.005






Station 10 - Elk River,
Can 5







6-24
1325
Surface
19
26
7.66
17
.01
 19
.17
<
.01
.02
27.00


10

28
8.05
.18
ND
1.07
1.07

.81
ND

7-15
1205
Surface
18
24.9
1.5 10.79
.26

1.29
1.27

.41
.02
71.25


10

24.6
1.7 10.53
.22

i.4o
1.38

.47
.02

9-16
1100
Surface
14
24"
8.44
.35
ND
.69
.61

 36
.08
99-00


10

24
8.48
 37
ND
.83
 75

.40
.08






Station 11 - C and D Canal, Nun 26







6-24
1335
Surface
20
25.5
8.20
.20
.004
.47
.43

 77
.04
27.75


10

27
7.79
 32
ND
.47
.45
<
.01
 03
89.25
7-15
1350
Surface
20
26
10.27
 29

1.09
1.07

 55
.02

10

25.2
9.00
.24

1.86
1.83

 56
.02

9-16
1145
Surface
14
24
8.13
.40
.01
.81
 79

.43
.02
99-00

10

24
8.07
51
ND
.90
.87

.43
 03






Station 12 - Elk River,
Nun 6







6-24
1430
Surface

28
7.25 11.U-
.20
ND
1.18
1.15

.20
 03
87.00
7-15
1410
Surface
18
25.4
9-3 13.98
 32

2.17
2.16

LA
.009
123.75
9-16
1115
Surface
12
24
6.9 10.77
.49
ND
1.12
1.10

.13
.02
123-00
ND - Not detectable
LA - Laboratory accident
vo

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1969
HEAD OF THE CHESAPEAKE BAY TRIBUTARIES
Date	Time	Sample Secchi Water	Total P PI	Organic N02+N0	NH~ Chloro- Phaeo-
Sample Sample Depth	Disc	Temp. Field Cond. DO	mg/l mg/l TKN	N	phyll a pigments
Taken	Taken	Feet	Inches	C	pH ymhos mg/l	PO^	PO^ mg/l mg/l	mg/l	mg/l	tig/l	M-g/1





Station 13 - Elk River,
, Nun 14





6-24
1415
Surface
14
31
7.0 9.88
 33
ND
.94
 90
.22
.05

7-15
1420
Surface
14
26.6
9.3 14.30
.44

.72
 71
LA
.007

9-16
1125
Surface
12
24
7.5 10.40
.50
ND
1.13
1.13
ND
ND
120.71





Station 14 - Northeast
River,
Nun 2





6-24
1500
Surface
38
25
8.63
.22
ND
.43
*27
.17
.07
37.5<

10

30
7-95
.20
ND
.70
.64
.01
.05
40.5c
7-15
1025
Surface
36
24.26
.08 8.78
.14

.68
.67
 36
 03
10

23.9
8.58
.11

1.01
 97
 39
.04
67.50
9-15
1115
Surface
24
23.5
9.80
.16
ND
.66
.65
.15
.005
15

23
9.08
19
.001
.61
.60
.13
.009






Station 15 - Northeast
River,
Nun 10





6-21+
1510
Surface
25
26
9.0 11.28
 29
ND
 91
.89
< .01
 03
70.50
7-15
1035
Surface
22
25.3
9.82
 17

 97
93
.09
.04
50.25
6

24.45
8.94
.17

1.00
.98
.11
.02
116.25
9-15
1130
Surface
15
23.5
10.68
.24
ND
.87
.85
ND
.02





Station 16 - Northeast
River,
Nun 18





6-24
1525
Surface
2b
27.5
9.2 11.32
.20
ND
1.00
97
< .01
.02
78.00
7-15
10^5
Surface
18
25.5
8.3 10.33
.20

1.62
1.00
ND
.01
69.00
8

23.6
7-99
.23

1.16
1.15
ND
.01

9-15
1150
Surface

24
7-3 8.67
.29
ND
 72
.72
ND
.008
102.75
10

24
6.6 7-36
.02
ND
.86
.83
ND
.02






Station 17 - Northeast
River,
mouth of
creek




6-24

Surface

26
7.9 11-09
 15
ND
.50
.42

.08
45.00
7-15
1100
Surface
22
25.3
7-2 9-69
 19

.62
.61
ND
.01
57-75
9-15
1155
Surface
15
24
7.4 8.25
 30
ND
.85
.85
ND
ND
117.75
ND - Not detectable
LA - Laboratory accident

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WATER QUALITY SURVEY
HEAD OF CHESAPEAKE BAY TRIBUTARIES
o	i	t	a	
APPENDIX

-------