ATHENS/GULF BREEZE ENVIRONMENTAL PROTECTION AGENCY
COOPERAIVE INDUSTRIAL WASTEWATER BIOMONITORING PROJECT

Status Report: Through FY1978

U. S. Environmental Protection Agency
Surveillance and Analysis Division
Athens, GA 30605

UNITED STATES
ENVIRONMENTAL PROTECTION AGENCY
SCIENCE AND ECOSYSTEM SUPPORT DIVISION
REGION 4

&EPA	

ALAN G. AUWARTER, PH.D.

Ecological Assessment Branch
980 COLLEGE STATION RD.

ATHENS, GA 30605-2720	(706) 355-8704

AUWARTER.ALANOEPAMAIL.EPA.GOV FAX (706) 355-8726

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Table of Contents

Introduction 		1

State Culture and Handling of Saltwater Test Animals

1.	Static Culture Methods 		2

A.	Cyprinodon variegatus

B.	Mysldopsis bahia

2.	Transportation 		5

3.	Acclimation 		6

Mobile Toxicity Test Trip Protocol

1.	Preparations 		7

2.	Flow-Through Tests 		7

3.	Static Tests 		8

4.	Chemical Analyses 		9

5.	Data Analysis 		9

Test Results and Discussion 		10

An Early Evaluation of anAcute Toxicity Test

Using Luminescent Bacteria 		20

Appendix 		23

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1

INTRODUCTION

Flow-through acute toxicity tests have been conducted on industrial
wastewaters at twenty-one sites throughout the coastal Southeast from
the inception of this interlaboratory effort through Fiscal 1978.

Static acute toxicity tests, effluent toxicity evaluations using
luminescent bacteria and chemical characterizations were run in parallel
with flow-through tests on-site or at the Athens laboratory in some
cases. Algal assays were conducted at the Gulf Breeze Laboratory on
effluent samples shipped from most sites.

The scope of this report is limited to providing basic information
pertaining to methods used, applicability of Cyprinodon and Mysidopsis,
the relative toxicity of wastewaters evaluated to test animals, and
early evaluation of a bacterial assay method in use over the past
6 months.

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2

CULTURE AND HANDLING OF SALT WATER TEST ANIMALS

1. Static Culture Methods

Salt water static culture facilities have been established at the
EPA laboratory in Athens, Georgia, for the purpose of supplying animals
for our on-site acute toxicity testing. Sheepshead minnows (Cyprinodon
variegatus) and a species of mysid shrimp (Mysidopsis bahia) were
selected as test organisms. Original and supplemental stocks have been
supplied as necessary from the Gulf Breeze Laboratory.

Athens cultures were^originally established using synthetic salts

R	R

(Instant Ocean and Rila Marine Mix ) dissolved in locally available

well water. A poor hatching rate of sheepshead minnow eggs prompted a
culture medium change to natural seawater.

To establish and maintain cultures in natural seawater, full-
strength seawater was periodically transported from coastal areas to
Athens and diluted with well water to culture salinities. Originally,
unfiltered seawater was used, but introduction of miscellaneous marine
organisms dictated a need for filtration. Filtered (1 micron) sea-
water has proven to be a satisfactory culture medium, assuming no
contamination from the seawater source.

A. Cyprinodon variegatus

Sheepshead culture in Athens involves three distinct steps:

1. Brood fish are held in 70-liter aquaria (61 x 33 x 41 cm) at
a stocking rate of 1-2 males and 5-6 females per aquarium.
An external power filter using activated carbon/plastic
floss media and a heater is used in each aquarium. Water

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temperature is maintained at 28-30°C. Egg collection trays
are formed by sewing together two layers of Nitex screening
of different mesh sizes. Trays cover the floor of each aqua-
rium, and are weighted with lengths of stainless steel tubing
attached underneath. Eggs are demersal and slightly adhesive
on the screening; they fall through the larger mesh of the
uppermost layer and are held between this and the lower,
smaller-mesh layer. Predation on eggs by adults has not been
observed using this type of tray. To collect eggs, a tray is
withdrawn from its aquarium, inverted over a shallow glass
pan containing salt water of the appropriate salinity to a
depth of about 3 cm, and agitated gently. Eggs fall to the
bottom of the pan from which they may be easily collected
with a pipette. Eggs may be counted as they are removed from
the pan or their number estimated by volume displacement:
100 eggs have been found to displace about 0.21 ml. Sheeps-
head in Athens cultures are depositing 80-100 eggs per fe-
male per week. Eggs are collected twice each week. Adult
sheepshead are fed frozen brine shrimp (Artemia sp.) to
satiation daily. To avoid dietary deficiencies, the brine
shrimp diet is supplemented with a commercial tropical fish
flake food.

Eggs are incubated and hatched in 4-liter wide-mouthed glass
jars. Jars are immersed in a water bath heated to 28-30°C;
vigorous aeration within jars keeps eggs and hatched fish in
suspension. A population of newly-hatched brine shrimp is

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4

maintained in each jar to feed developing fry.

3. Fry are held in 7-liter all-glass aquaria segregated
by date of egg collection and salinity from the time
they are two weeks of age until they are either used
in tests or otherwise disposed of. These aquaria
are supplied with bubble-up type activated carbon/
plastic floss filters; water temperature stays at
about 17°C. These fish are fed newly-hatched brine
shrimp daily. Unusually large individuals are re-
moved from each aquarium periodically to avoid preda-
tion on smaller fish.

B. Mysidopsis bahai

Complete life cycles of this mysid shrimp species are
maintained in 70-liter aquaria at about 17°C. Each aqua-
rium has a commercially available under-gravel filter which
is covered with about 5 cm of small, smooth sheel fragments
(4-15) mm in length). To avoid extensive algal growth, the
walls of the aquaria and filter standpipes are scraped and
shell substrate is periodically turned over to partially
bury the existing algae. Velocity of water returned
through filter standpipes is sufficient to maintain a
moderate current in the aquarium. The shrimp often orient
themselves into this current. Mysids are fed with newly-
hatched brine shrimp twice daily. Frequent feeding is
necessary to avoid cannibalism.

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Transportation

Two days prior to departure for a bio-assay trip, standard size
(36 X 30 X 30 cm) styrofoam coolers are prepared for the transport of
test animals. Each cooler is supplied with two doubled plastic bags
(56 X 32 cm), and each bag is filled with 1-3 gallons of synthetic salt
water at a salinity of either 10 or 15 parts per thousand. Mysid
shrimp are bagged by culture salinity, 50-200 individuals per bag.
Sheepshead are bagged by culture salinity and age grouping, 200 to
500 fry per bag. All bags are moderately aerated with air stones
powered by a Silent Giant for these two days.

The morning of departure, minnows and shrimp are carefully netted
from culture or holding tanks and released into the appropriate pre-
labeled plastic bag. Each bag is liberally stocked with newly-hatched
brine shrimp to serve as food for test animals during transport. An
additional supply of hatched brine shrimp and eggs is transported in a
well-aerated gallon jar to prevent a lapse in food supply before Artemia
cultures can be established at the test site. Air supply to the plastic
bags is rerouted from the AC-powered pump to battery-powered aerators and
the bags are closed with pipe cleaners. Animals are transported in a
passenger vehicle rather than the test trailer to reduce temperature
fluctuations and other environmental disturbances.

After arrival on-site, test organisms are transferred to the trailer
where they are once again aerated with an AC pump. If necessary, addi-
tional laboratory-hatched brine shrimp are added to ensure an adequate
interim food supply, and a fresh brine shrimp culture is started on-site.

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Acclimation

On site acclimation of animals to local water is initiated as soon
as suitable marine dilution water source has been located. A five-gallon
sample of this seawater is transported to the trailer and diluted with
well water (from the Athens laboratory or supplied by the plant being
tested) to salinities of 10 and 15 parts per thousand. Half of the water
volume from each bag of test organisms is removed and replaced with an
equal volume of dilution water of the appropriate salinity. Partial
volumes should be replaced in this way so that the holding medium for
test animals is changed from 100% laboratory holding water to close to
100% locally available dilution water over a period of 24 hours. Or-
ganisms should be exposed to 100% dilution water for at least 24 hours
prior to testing.

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MOBILE TOXICITY TEST TRIP PROTOCOL

1.	Preparations

Trip preparations include packing truck, trailer and test organisms
for travel. A pre-trip check of equipment and supplies packed is made
against a detailed checklist.

Upon arrival at an industry, in addition to immediate electrical
hookup, suitable dilution water is sought, and where possible, collected
that day. This water should be low in suspended particulate matter and
industrial contaminants to the extent possible to determine in the field.
When adjusted to proper salinity (if necessary) using suitable well water,
this water is used for the acclimation of test animals and in the formu-
lation of solutions for toxicity testing.

Twenty-four hour range-finding static bio-assays using a limited num-
ber of animals and wastewater concentrations are initiated as soon as
possible after arrival on-site. Results of these tests serve to estab-
lish the concentration range(s) to be used for definitive toxicity tests.

2.	Flow-Through Testing

The formal flow-through test begins on the third day. Ten shrimp
or fish are counted out into each of 12 plastic weigh boats. Animals
are observed for signs of abnormal behavior or other indications of poor
physiological condition, and any such individuals are replaced. Indi-
viduals markedly larger or smaller than the population mean within each
boat are likewise replaced to limit predation. One boat of test animals
is then released into a screened chamber within each of the 12 aquaria
used in toxicity testing. Aquaria containing 100 percent effluent are

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infrequently used in testing since most effluents result from fresh
water used in industrial processes, and therefore cannot be expected
to support marine life without addition of or dilution by salt water.
Animals are observed frequently on the first day of testing so that
early counts can be taken for the purpose of LC50 calculation if the
effects of the effluent are severe, and early mortality rates high.
Counts of animals surviving in each test chamber are made routinely
at the end of each of the four 24-hour periods comprising one 96-hour
test.

Static Testing

One day prior to the initiation of the 96-hour flow-through test,
an Isco Model 1580 sampler is set up to collect a 24-hour effluent com-
posite totalling approximately 20 gallons. Up to fifteen gallons of thi
composite are sent to the Gulf Breeze laboratory for additional toxi-
city testing; five gallons are used on-site to formulate static test
effluent concentrations reflecting those produced by the flow-
through diluter system. Sheepshead minnows are tested in quart
jars containing 750 ml of solution; mysid shrimp are tested in gallon
jars containing 3000 ml of solution. Ten organisms are placed in
each effluent concentration and static containers are slowly aerated
(single-bubble) throughout the testing period. Statics are conducted
for 96 hours. Initial water quality parameters, including dissolved
oxygen, salinity arid temperature are recorded for each test container.
Dissolved oxygen values are monitored daily, and values for pH and total
alkalinity are collected at the conclusion of the 96-hour testing period

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Chemical Analyses

Chemical analyses of test water involves continuous monitoring of
undiluted effluent and daily aquarium checks. At sites where effluent
flow to the trailer is sufficient, flow to the diluter is split and a
portion is passed through a Schneider monitor/strip recorder to track
pH, temperature, dissolved oxygen and conductivity of incoming efflu-
ent during the course of the study. Also a set of remote probes for
temperature and pH is used in a selected test aquarium. This monitor
system is electronically calibrated in Athens, and field checked both
before beginning and periodically during each study. In addition to
continuous monitoring, dissolved oxygen, temperature and salinity are
recorded from each test aquarium and samples are taken from each efflu-
ent concentration for determination of pH and total alkalinity each day
during the course of the flow-through testing.

Data Analysis

Post-test data analysis includes mathematical evaluation of the
extent of wastewater toxicity to test animals. Whenever the pattern of
mortality over test concentration permits, LC50 values calculated by
probit analysis are reported including 95 percent confidence limits.
Where probit analysis is inappropriate, LC50 values are determined by
graphical interpolation using the log-concentration-versus-percent
survival method. Where mortality exists but is too heavy or light to
be described by standard LC50 values, a value such as 80/5.6 is re-
ported: 80 percent mortality in 5.6 percent wastewater after an in-
dicated exposure period.

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10

TEST RESULTS AND DISCUSSION

Information pertinent to these on—site studies is summarized in
Table 1. Entries are arranged in order of decreasing toxicity on the
basis of 96-hour flow-through mysid test results. Test results from
the SCM Cooperation are eliminated from the following considerations
since temperature regulation problems resulting from trailer air-con-
ditioning failure during summer temperatures in Florida created im-
possible conditions under which to conduct a valid study.

The degree of toxicity of wastewater apparently cannot be grouped
by industrial category. Wastewater from the four kraft paper producers
showed a high degree of toxicity (Container Corp., Westvaco) , an inter-
mediate degree of toxicity (International), and no toxicity (Brunswick)
to mysid shrimp as compared to other industrial wastewaters tested.

Further, of wastes from the two kraft producers demonstrating the
highest toxicity to mysids, one (Container Corp.) showed high toxicity
and the other (Westvaco) showed no toxicity to sheepshead. The two
plants having wastes influenced primarily by dyestuffs are similarly
split between the high (Verona) and low (dePoortere) ends of the scale
of waste toxicity to mysids. Of the two Dacron/Dacron precursor plants
visited, wastewater from one (Hercofina) was highly toxic, wastewater
from the other (duPont) was non-toxic to the animals tested. The two
plants producing agriculturally-related products discharged wastewaters
that were among the most toxic to mysids. One (Grace) , a fertilizer
producer, was also higly toxic to sheepshead; the other (Stauffer,

Cold Creek), a biocide producer, was non-toxic to sheepshead. Toxicity
to specific aquatic organisms, or, specifically the lethality of

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TaM e

Summary of Information From l IHJ I .1. I S l~ I WW II.HI S of tll(J
MIilm^/GiiI f Bree/.o F.PA Cooperative liulust.ri.il On-site Acute Toxicity TesLlng Program

I nduti l ry
Add r< -.s

N.i jor
Prodiu is

S L < icly
Dates

PI.Kir Diseharf.e
Vo I ume (MCD)

Test Results

he

Cypr 1 notion v.i r i uy,a uis

My s i d op si s bain ,i

Reeontmend*. il

Kercivm^ 		~

Waterway	i: 1 ow-1 hroni'.h Tcs t S t a L i c 'i'es I K1 ow-through IVs.^ Static 'lest. Action"

H. (.r.u e «k. Co.
1 rr>i uj»t on, NC

.!>; t* n. u 11 ur.i 1
fcrtiJlzcrs

7/26

to
8/6
1977

0.07-0.

N . U. Cape
Fear River

96 ii =	15.6

72 ii =	17.0

48 h =	18.0
24 ii = '32.9

Suh-nclul L

96 1. =	38.8

72 ii =	38.3

48 h =	38.3

24 ii =	A 2.6

48 h = 20/60
24 Ii = N.M.

96 ii = 100/5.6
72 h = 95/5.6
48 ii = 80/5.6
24 h = 11.6

Youn&
24 h = 100/5.6

48 ii = 20.0
24 h = 60.0

auffer Cliem. Co.

r u" u 1 turj i chem. ,

9/9

0. 1 1-0.40

Mob Lie RLver



N

.S



	

96

h

=

75/5.6

Id Creek Pl.int

he rh i c ides and

Lo















72

h

=

13.3

i.ks, A1,

pesi J c ides

9/15















48

h

=

26.0





1977















24

h

=

28.1























Young





















72



=

12.2





















48

ii

=

17.6





















24

ii



30.9

nta iiu-r C<»rp.

kriift papor

2/2

18.0-21.1

Ame1ia River

96

ii

s

7.7

N.M.8

96

ii

=

5.9

r ii. md i m.i 1' i li.



l o





72

li

=

7.7



72



=

5.9





2/9





48

ii



3(1. 1



48

it

=

18.0





l'J78





24

it

-

42.3



24

ii

=

19.3

orican Co I or

d y e i ii t e. r med i a t e s

12/9

0.25-0.33

Cnmpbel1's

96

h

s

5/32

	

96



=

6.3

d riifin. Co.



to



("rue k

72

ii

=

5/56



72

h

=

6.4

bee o t SC



12/16





48

li



N.M.



48

li

=

19. 1





1977





24

ii

=

N.M.



24

ii

=

42.3











Fish

26

-28 days old





















96

it

=

41.6



96

it

=

80/10











72

Ii



48.9



72

ii

=

60/10











48

ii

=

49.0



48

li

=

1 1 .0











24

ii



5/56



24

h

=

23.9





















72

ii

=

8.01





















48

Ii

=

15.7





















24

ii

=

21.7

StV.lt O Corp .

iinbliMi ii* il k ra f t p apt r ,

4/29

27-30

Cooper River



N.M.

	

96

h

=

8.7

aft Division

pro; ess flu mica 1

lo















72

1)

=

13.1

Cha r 1 us ton ,

roc 1 jnui ion

5/6



























19/7























rcof ina Corp.

IPA I DM I (two

7/19

0. 18-0.25

Cape Fear and

96

h

=

21.3

N.M.1

96

ll

=

9.6

1 in L ji^, t <>n , r,C

|)ac run precursors)

to



N. E. Cape

72

ii

=

24.1



72

ll

=

9.6





7/24



Fear R ivers

48

li

=

25.7



48

ll

=

9.6





19 7 7





24

h

=

39. 7



24

ll

=

12.9























Younj;





















48

h

=

/. I





















24

ll

=

7.4

72 ii « 50/56

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Indust ry
t,

	Add rr'i'i _

Mobay < Ik >ii. (\»j |>.
Vt-ron.i l)ye-.t m f
IJI v i s i on
Cliar lisimi, SI,

Into rn.i l i ihi.i I
Paper Co.
Georgetown , S

Ct;i r J i n i c r Clicm.
Tampa» Fl,

M.i |or
Produc ls

(lVi

M cached and
mib I e.ichcd kra f c,
nouL ra 1 svi 1 f l ce
process paper

pin >-.plior i l pn>dik' ls

S I 11 d v

0^1 L-s

r>m

Lo

5/29

I 97 7

8/12

to
8/18

1977

6/14

to
6/22

1978

Pl.inL Discharge
Vol nine (M("D)

3.5-5.2

Til'

Rece iv i ng
W.i Lerw.iy

Cooper Kiver

it I 111 i t?cf

29-31

(60-9- min.
release, twice
daily)

Sampit River

1/001
#005
#006

21 .6-2 3.2 Al.ifia River
18.8

0.18-0.30

Test Results

be



Cvprinodon variegatus



Mvsldopsis bahiaL>

Rlm onunt j nil









K1ow-1hrough TesL Static Test

F1 ow

-through Test Static Test

Ac; L i_on _

96 1

= 16.4

96 li

= 22.5

A

72 1

= 28.0

7 2 h

= 24.0



48 1

= 34.8

48 h

= 29.2



24 1

= 46.7

24 li

= 75.0



96 1

= 15/56 N.M.

96 h

= 28.8k N.S.M.

None

72 1

= 15/56

72 h

= 31.8



48 1

= 15/56

48 h

= 33.6



24 1

= 15/56

24 h

II

00
UJ





N.M. N.M.

96 h

ll

u

1

1
t

1)



(3 tests)

72 li

= 82.5



Staulter Chein. Co.
leMoyne l'lant
Axis, Al.

I	ndusI r ia 1 grade clieni.

II	,SO, , CXI, , CI.,, CS,
NaOII 'and others'

9/15
to

9/22
1977

1.5-2.0

Mobile River

N.M.

96 li = 45.4
72 h = 4 5.4
48 h = 15/56
24 h = 10/56

None

Relchltold CluMiieal tall oil products
Bay Ninette, Al,

5/1
to

5/8
1978

0.018-0.041

Hoi]tnger
Creek

N.M.

N .M.

96 h = 50.0
72 h = 40.0
48 h = 32.0

72 h = 40.0

0 1 in Clicitii <:a 1

smokeless powder,

1/12

0.33-0.52

liig boggy

N.M.

N.M.

96

h =

45/56

St. Marks, Fl,

n i t ro-^1yee r i ne

to



Branch



(2 Lests)

72

h =

15/56





1/19









48

h =

30/56





1978









24

h =

25/56

Georgetown St oe 1

steeL wire and rod

3/20

0.65-0.67

Sampit River

N.M.

N.M.

96

li =

20/56

Georgetown, SC

to
3/27
1978

N.S.M.

None

None

Pfizer Cliem lc.i 1
Sout hport f NC

eitrie ncid

7/6
to
7/13
1978

0.71-2.43

Cape Fear River

N.M.

N.M.

96 h = 20/56

Norn.-

Virginia Cbem.
Bucks, Al.

sod L inn hyd rosu 1 f* 1 tc ,
a I i phat i c am l nes

4/24

to
5/1
1978

0.009-0.011	Cold Crock

N.M.

N.S.M.	96 h = 15/56

Brunswick Pulp
awd Paper
Bmnsw i i k, GA

bleached 6 unbleached
market kral't paper

8/7

to

8/n
1 7 B

54-59

Turtle River

N.M.

N.M.

N.S.M.

N.S.M.

Nnn«-

E. I. dnPont dr

Nl'IIU II 11 . h (,i >.

WI I m j nj;l on , N('

TPA, DM I and
I) M r < mi po I ¦ I ¦
I i I -I r

8/1

I O
«//
r>7 /

1.7-1.9

Cape Tear
l< I vi-r

N.S.M.

N.M.

N.S.M.

N.S.M.

-------
Table

i nucd

Test Results*10

I nc 111 .lry
t,

	 A1111 r«

dePooi ten:
Coi poi at ion
WI I in I ngt on , NC

D1 .imoiul
Sbamro< k

Cast I u Hayue, NC

Genera 1 I-1 eel r i c
Wi1 ming t ()n, 11C

Ma jor
	Pj^'K tj

woven i l\iuLti'if
1. i h r i i b ; dyeing

x-d i uni b ii lir<>niat«.
rhroju i c acid

niu 1 ea r f ue I
pe 1 Ie Ls, me ta I
file I pi- 1 luLS

Sludv
lUL^s

M/4

I o
1 1/10

1977

7/1 J

to
7/JO

1978

CO

11/17
1977

Plant 0irge

Vo 1 unie	(MCO)	

I.04-1.22

Kece i v i ng
	Waterway	

Smith Creek

Cyj>r hlodon vai Jega t us

0.48-0.60	N. E. Cape

Fear Kjver

0.62-0.70	N. I!. Cnpe

Fear River

1' 11)w-t hroug11 To!'.l St £i_Lj c TesI
N.M.

N.M.	N.M.

N.M.

N.M.

Mys id opsls bal > i a

"	Ki'« oimiu nded

Flow-through Test	Aetinn"

None

None

	 Test Results fnconclusive

SCM Cor|i.	>,i u'li Ls , fhivuriiiKS 6/23 2.7-3.3 Moncrlef Crcuk	Test Results I nconc 1 usivu : Random Mortality Throughout

G1 idden-Durkee Div.	to Concentration Range.

Organii ( In in. Croup	6/29

Jacksonv i 1 I e , I I-	197 7

«> • Unless otherwise indicated, all information presented in this table has been reported in Individual, industry toxicity test reports.

1>. Single values reported are f.C50 values.

c.	l/her<. LC50 values could not be mathematically derived, the percent of test animals that died at the lowest test concentration in which mortality
occurred is given and is to be read as follows: 20/5.6 represents 20 percent mortality in the 5.6 percent concentration of wastewater.

d.	Unh -,h otherwise stated, nil Cyprinoclon used were 1-3 weeks old. "Sub-adult" Cypr 1 nodon were 13-20 mm in length, age unknown.

e.	Unless otherwise stated, all M\ s i dops ls used had reached full length (6-8 niin) and were not further identified by sex or age. "Young" mysids were
1.5-2 ii.i.) in length and 1-3 days old.

i. N.S.M.= j\o s i gn i f i c ant inort.i 1 j Ly.
g. N.M.= No mor ta 1i ty.

A 70: 10 mi Uuri ol Mys i dops i s l> i go 1 ow i and Neomys i s americana was used in this test,
j- 'Usi couduitcd in Alliens using well water collected on November 10, 1977 , substituting 6-month-old Lopomis niacrochi rus for Cyprinodon var i i-gatus

a s t he test organ i sm.
k. Test conducted substituting l-3-day-old Paphnia magna for Mysidopsis bahia.

!• Additional static tests were conducted with fresh water species (effluent salinity 3 mg/ml or less).

1'p mine 1 .is : no mortality after 96 hours.	D.iphn i a pu 1 ex : 48 hour L.C50 - 32.0 percent.

,n« Test cundik ted using fingerling I eta 1 urns punctatus for Cypr jnodon variegatus as the tesL organism,
l).	See following n.ii'c.

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Tab I

mod

Specific AcLions Recommended to KPA Knforceinent Division for
Region IV Discharges liased on Results of On-site Acute Toxicity Tests.

Kci oistiietuK'il .ii t ion :

-	r«quire the industry to determine how rapidly and uniformly their wastewater mixes with the receiving water.

-	ll w.istiw.iii r not undergoing rapid mixing, require industry lo install diffuser pipes in the receiving water.

U, < <»n no!id. il .1 c L i on :

-	require I he company to conduct dye studies to determine the isopleths of wastewater concentration and submit the results displayed on a map of
! Ik1 rou i v i ng w;i ter .

require the company to provide UFA with their daily wastewater discharge flow in MOD.

-	on< e the wastewater concentration (toxicity limit) at the bound,iry of the mixing zone has been established, an effluent toxicity screening test
'.hould be required of the company using the calculated toxicity limit.

Krcomiik-nded at 11 on :

-	company should conduct dye studies ti> define Clio flow characteristics (plot isopleth of wastewater concentrations) of the wastewater as it
enters and mi .-.os with the receiving wjter.

Rei.oiiiiik-nded ac L i on :

-	inquire that clie wastewater be discharged via a diffuser pipe for rapid and homogeneous mixture in the receiving water.

-	compute tlu; 7l,U0 flow at a point in the Alafia River near the plant outfall for use in determining the extent of dilution of wastewater.

-	imm the extent of dilution determine the zone (area) of chronic toxicity.

-	(he inl di'iii.i t ion on the area affected by chronic toxicity can bo used by the regulatory agency to determine if and how much reduction ol waste-
water toxicity may be required by the plant.

K» commended ac t it'll:

-	company should provide EPA with calculated dilution ratios of their wastewater based on low flow conditions at selected locations downstream
from the I r discharge.

Recommended ac t i on :

-	no further blomonitoring unless the company's wastewater characteristics change due to plant production changes.

-------
15

industrial wastes, is more likely related to the interaction of several
factors including:

-	wastes produced,

-	in-plant waste treatment,

-	final concentration of wastes when released, and

-	species-specific physiological response to each
complex effluent mixture.

Characterization of organics in wastewater using GC/MS analysis is
reported for three plants in addition to those reported last year
(Table 2). These three followed a trend established as a result of GC/MS
characterizations for the six previous samples: wastewater toxicity seems
to be related to both the number and total concentration of identified
organic compounds. Organic analyses were discontinued as of
September, 1977. Results of inorganic analyses are given in Table 3.

Recommendations to Region IV1s Enforcement Division, based on these
test results ranged from no action to the determination of isopleths
depicting waste flow into the receiving waterway, determination of 7Q10
values at the point of discharge, possible installation of diffuser
pipes in receiving waterway, further toxicity testing and possible re-
duction of toxicity of effluents based on the resultant information,
each to be conducted by the industry (Table 1).

Flow-through tests have produced much more information to date in
this project than have static tests. Of all flow-through tests conducted
with salt-water animals, 63% have produced reportable toxicity (mortality
relatable to wastewater concentration) , and 51% have been at least toxic
enough for the calculation of LC50 values. Of all on-site static tests

-------


TabLe 2





GC/MS

Characterization of Wastewater and Dilution Water Used

for Testing at Each Industrial Site







Concentration(mg/l)

Industry

Compound

Effluent

Dilution

Westvaco

Di(2-ethyl hexyl) Phthalate

330

<5



Phenylacetic Acid^

110

ND



5 unidentified fatty acids

approx. 5

ND



2 unidentified resin acids

approx. 5

ND



oil

unspecified

ND



grease

unspecified

ND

Verona

Chloroaniline

52

ND



Chloronitrobenzene

180

ND



1,3,3-trimethyl-2-methylene indoline

170

ND



Diniethyl-2-methylene indoline

5.9

ND



1, 3,3-trimethyl oxindole

220

ND



Unidentified Phthalate

58

ND

SCM

Hereofina

Grace
duPont

International Paper

organic samples discarded: toxicity test results inconclusive

Ethyl hexanoic acid
P-Cresol

Phenyl acetic acid

Phenol

Indole

Alkyl C3 Benzenes^
di(2-ethyl hexyl) Phthalate
Isobutyric AcidT
n-Butyric AcidT
Valeric Acid^

Laurie Acid^

Phenyl IsoeyanateT

no organics present within detectable limits
Ethyl Toluene
Xylene

N-Propyl Benzene
N-PenteneT
Dimethyl Butane^

Unidentified Phthalate
Lube-type oil

180
190
200
110
95
60
19
130
160
560
130

140

8.5
1.5
1.8
10
10
28

unspecified

ND
ND
ND
ND
ND
ND

trace(<5)
ND
ND
ND
ND

ND
ND
ND
ND
ND
29
ND

-------
Table 2, continued

GC/MS Characterization of Wastewater and Dilution Water Used for Testing at Some Industrial Sites

Concentration (ug/1)

Indus try	 	Compound	Ef f luent	Dilution

Stauffer Cycloate	19	NA

(Cold Creek) Fonophos	3.2	NA

Phosmet	ND	ND

Triethylphosphate	45	NA

Bis (2-ethy] hexyl) phthalate	2.3	NA

EPTC	37	NA

Butylate	2.4	NA

Vernolate	7.7	NA

Pebulate	0.71	NA

Molinate	40	NA

Stauffer Cycloate	1.2	NA

(LeMoyne) Fonophos	ND	NA

Phosmet	ND	ND

Triethylphosphate	ND	NA

Bis (2-ethyl hexyl) phthalate	6.6	NA

EPTC	1.2	NA

Butylate	0.11	NA

Vernolate	0.18	NA

Pebulate	ND	NA

Molinate	4.9	NA

American Color Methyl phenol (isomer)	14	ND

Benzamide	120	ND

Phenyl carbamic acid, methylester and/or isocyanatobenzene	21	ND

Dihydroxy anthraquinone	81	ND

Aniline	2500	ND

Methyl aniline (isomer)	500	ND

Chloroaniline (isomer)	270	ND

N-nitrosodiphenyl amine and/or diphenyl amine	23	ND

Nitro aniline	40	ND

Di-n-butyl phthalate	6	ND

Anthraquinone	20	ND

Aminoanthraquinone	of	Mn

T =

tentative identification

NA = not analyzed

ND = not detected

-------
Tab i u J

Inorganic Analyses for Samples oT Industrial Wastewater fiml Dilution Water'1'1





NH3-N

TOC

Phenols

Zinc

Chromiu™

Copper

Lead

Cadmium

Industry

Sample

(me /1)

(me /1)

Cii p /1)

(rng /I)

(me /I)



(mg/1)

(mg /I)

Westvaco

Eff.

0.39

94

17













Dil.

0.02

8

15

-

-

-

-

-

Verona

Eff.

6.70

44

75

-

-

-

-

-



Dil.

0.03

9

14

-

-

-

-

-

SCM

Eff.

-

-

-

-

<80

57

-

-



Dil.

-

-

-

-

<80

34

-

-

Hereofina

Eff.

6.0

—

-

43

31

<20

101

<20



Dil.

ND

-

-

56

50

35

247

22

Grace

Eff.

14

-

-

-

-

-

-

-



Dil.

0.31

-

-

-

-

-

-

-

duPont

Eff.

0.04

-

-

43

88

-

-

-



Dil.c

0.04

-

-

55

<50

-

-

-

International



















Paper

Eff.

1.10

330

21

75

<80

24

<80

<10



Dil.

0.20

3.3

<5

80

<80

45

296

28

a.	Effluent samples are of unadulterated material.

b.	Dilution water is either estuarine water collected at the necessary tidal phase to be within
the range of, usually, 15-18ppt salinity or is a mixture of higher salinity seawater and well
water to provide a solution within the same salinity range.

c.	Flow-through tests were run using fresh water. Dilution water characterization given is,
therefore, for fresh dilution water. Values for dilution salt water, used in static tests,
are non-detectable, 22 and 41 mg/1 for NH3-N, zinc and chromium, respectively.

-------
19

conducted with salt water animals, 17% have produced reportable toxicity
and 8% have been toxic enough for the calculation of LC50 values.

Mysidopsis bahia has been a much more useful test species than
Cyprinoaon variegatus. Of all on-site toxicity tests conducted with
Mysidopsis, 78% have produced reportable toxicity, and 63% have been at
least toxic enough for the calculation of LC50 values. Of all on-site
toxicity tests conducted with Cyprinoaon, 24% have produced reportable
toxicity, and 16% have been toxic enough for the calculation of LC50
values.

On-site sheepshead statics have never resulted in mortality suffici-
ent for the calculation of LC50 values, and in only one test (6%) of the
sixteen conducted has any toxicity been demonstrated through mortality.

Sheepshead flow-through testing produced reportable toxicity in 36%
of all tests conducted, and LC50 values could be calculated for 27% of
the tests. Mysid statics have resulted in reportable toxicity in 38% of
tests conducted, and LC50 values could be calculated for 25% of the
tests. Mysid flow-through tests have resulted in reportable toxicity
in 95% of the tests conducted, and LC50 values could be calculated for 74%
of the tests.

-------
AN EARLY EVALUATION OF AN ACUTE TOXICITY TEST USING LUMINESCENT BACTERIA

The Microtox™ is a specialized photometer being developed by Beckman
Instruments, Inc. and evaluated by this and other laboratories for its
applicability as a new acute toxicity testing system. Essentially, a
nearly instantaneous response is recorded as percent light increase or de-
crease from a suspension of luminescent bacteria upon exposure to a potential
toxicant. Potential benefits to a regulatory agency or discharger consci-
entiously evaluating toxic effects of a wastewater would include savings
of time and therefore expense and manpower, and a degree of uniformity
of test organisms greater than that possible with current popular test
organisms. Tests can take 30 minutes to 3 hours as opposed to conventional
96-hour tests that may take a total of 8 days in the field. Standard, cul-
tured bacteria may be supplied from a pure culture maintained in a single,
centralized laboratory. Vials of lyphilized bacteria are easily stored
and shipped.

This test system is in an evaluative phase in its development, and
problems do exist. One concerns data interpretation. A decrease in the
amount of light emitted by the bacteria can easily be interpreted as a
deleterious or "toxic" effect. An increase in the amount of emitted
light, analagous to "stimulation" in an algal assay, is more difficult
to categorize. Does such "stimulation" represent an enrichment of the
environment, and if so, should such be regarded as deleterious, accel-
erating eutrophication, or should it be regarded as a desirable situation.

A comparison of results of the bacterial test with results of stand-
ard on-site flow-through tests with Cyprinodon variegatus and Mysidopsis
bahia is given in Table 4. Entries are listed in order of decreasing
wastewater toxicity to Mysidopsis. The wastewater most toxic to mysids

-------
Table 4

Comparison of Results Between On-site Toxicity Testing With
Luminescent Bacteria and Flow-through Testing With Standard Test Organisms'

Test Organism

Industry

Sheepshead
Minnow
(96-hr exposure)

Mysid
Shrimp
(96-hr exposure)

Luminescent
Bacteria
(10-min exposure)

Reichhold

0/56

32

41/50

Gardinier

0/100

44

13% stimulation'3

Pfizer

0/56

20/56

18/50

Virginia

0/56

15/56

c

2% stimulation

Brunswick

0/56

0/56

10/50

Single values represent LC50 (sheepshead, mysids) or EC50 (bacteria)
values in percent effluent, unless otherwise indicated. Slashed values,
used when test mortality was insufficient for calculation of LC50's or
EC50's, represent:

percent mortality

highest effluent concentration tested

	percent light decrease	

highest effluent concentration tested

for sheepshead and mysids or

for bacteria.

Increase in light output after 10 min. exposure to the highest test
concentration of wastewater (33%) .

Increase in light output after 10 min. exposure to the highest test
concentration of wastewater (50%).

21

-------
22

(Reichhold) was also most toxic in the bacterial test. If a "toxic"
bacterial test result may be defined absolute deviation from no effect,
then Virginia Chemical and Brunswick Pulp and Paper wastewaters were
shown to be the least toxic by both the mysid shrimp test and the
bacterial test. If bacterial stimulation is defined to be other than
a toxic or deleterious effect, the comparison between mysid and bacterial
tests for these first five field-tested industries is not as direct.
Sheepshead minnow test results for these industries is included in
Table 4 to illustrate the relative insensitivity of this test species.

-------
APPENDIX

-------
23

Data Collected On-site to Date as Part
of Athens/Gulf Breeze EPA Cooperative Project

Table

#	Table

I. Biological Data and Chemical Parameters Recorded by Test Aquarium
During a Flow-through 96-hour Acute Toxicity Study Conducted at
Westvaco Corporation, North Charleston, SC, April 29-lIay 6, 1977.

II. Biological Data and Chemical Parameters Recorded by Test Aquarium
During a Flow-through 96-hour Acute Toxicity Study Conducted at
Verona Dyestuff Division of Mobay Chemical Corporation, Charleston,
SC, May 23-29, 1977.

III. Biological Data and Chemical Parameters Recorded by Test Aquarium
During a Flow-through 96-hour Acute Toxicity Study Conducted at
the Glidden-Durkee Division of SCM Corp., Jacksonville, FL 32201.

IV. Survival Data Recorded for Wastewater Concentrations During a Flow-
through Toxicity Study Conducted at Hercofina Corporation,

Wilmington, North Carolina, July 20-24, 1977.

V. Chemical Parameters Recorded for Wastewater Concentrations During a
Flow-through 96-hour Acute Toxicity Study at Hercofina Corporation,
Wilmington, NC, July 20-24, 1977.

VI. Survival Data Recorded by Test Aquarium During a Flow-through

Acute Toxicity Study Conducted at W. R. Grace & Co., Wilmington,
NC, July 25-August 2, 1977.

VII. Survival Data Recorded During 48-hour Static Acute Toxicity Testing

Conducted at W. R. Grace & Co., Wilmington, NC, July 25-August 2, 1977.

VIII. Ranges of Values for Chemical Parameters Monitored Periodically
for Each Wastewater Concentration Tested During a Flow-through
Acute Toxicity Study at W. R. Grace & Co., Wilmington, NC,

July 25-August 2, 1977.

IX. Survival Data Recorded by Test Aquarium During an Acute Toxicity

Study Conducted at E. I. duPont de Nemours and Company, Wilmington,
NC, August 2-6, 1977.

X. Chemical Monitoring Data Recorded by Test Aquarium During Flow-
through Acute Toxicity Testing at E. I. duPont de Nemours and
Company, Wilmington, NC, August 2-6, 1977.

-------
Table
//

Table

24

XI. Survival Data for Acute Toxicity Tests Conducted at International
Paper Company, Georgetown, SC, August 14-18, 1977.

XII. Chemical Parameters Recorded by Test Aquarium During a Flow-through
Toxicity Study Conducted at International Paper Company, Georgetown,
SC, August 14-18, 1977.

XIII. Biological Data Recorded by Test Aquarium During a Flow-through

96-hour Acute Toxicity Study Conducted at the Cold Creek Plant of
Stauffer Chemical Company, Bucks, Alabama, September 11-15, 1977.

XIV. Chemical Parameters Recorded by Test Aquarium During a Flow-through
96-hour Acute Toxicity Study Conducted at the Cold Creek Plant of
Stauffer Chemical Company, Bucks, Alabama, September 11-15, 1977.

XV. Biological Data and Chemical Parameters Recorded by Test Aquaria
During a Flow-through 96-hour Acute Toxicity Study Conducted at
the LeMoyne Plant of Stauffer Chemical Company, Axis, Alabama,
September 16-20, 1977.

XVI. Biological Data and Chemical Parameters Recorded by Test Aquarium
During a Flow-through 96-hour Acute Toxicity Study Conducted at
dePoortere Corporation, Wilmington, NC, November 7-11, 1977.

XVII. Biological Parameters Recorded for Wastewater Concentrations During
a Flow-through Study Conducted at American Color and Chemical
Corporation, Lobeco, SC, December 9-17, 1977.

XVIII. Chemical Parameters Recorded for Wastewater Concentrations During

a Flow-through Acute Toxicity Study Conducted at American Color and
Chemical Corporation, Lobeco, SC, December 9-17, 1977.

XIX. Survival Data Recorded by Test Aquarium During an Acute Toxicity

Study Conducted at the Olin Corporation, Smokeless Powder Division,
St. Marks, FL, January 15-19, 1978.

XX. Chemical Parameters Recorded by Flow-through Test Aquarium During
an Acute Toxicity Study Conducted at the Olin Corporation, Smoke-
less Powder Division, St. Marks, FL, January 15-19, 1978.

XXI. Survival Data Recorded by Test Aquarium During Acute Toxicity

Testing Conducted at the Container Corporation of America, Fernandian
Beach, Florida, February 6-10, 1978.

XXII. Chemical Parameters Recorded by Test Aquarium During Flow-through
Acute Toxicity Testing Conducted at the Container Corporation of
America, Fernandina Beach, Florida, February 6-10, 1978.

-------
25

Table

//	Table

XXIII. Survival Data Collected by Test Aquarium During an Acute Toxicity
Study at the Georgetown Steel Corporation, Georgetown, SC
March 22-26, 1978.

XXIV. Chemical Parameters Recorded by Flow-through Test Aquarium During

an Acute Toxicity Study Conducted at the Georgetown Steel Corporation,
Georgetown, SC, March 22-26, 1978.

XXV. Survival Data Recorded by Test Aquarium During Acute Toxicity
Testing Conducted at Virginia Chemicals Incorporated, Mobile,

Alabama, April 27-May 1, 1978.

XXVI. Chemical Parameters Recorded by Test Aquarium During Flow-through

Acute Toxicity Testing Conducted at Virginia Chemicals Incorporated,
Mobile, Alabama, April 27-May 1, 1978.

XXVII. Survival Data Recorded by Test Aquarium During Acute Toxicity

Testing Conducted at Reichold Chemicals, Inc., Newport Division,
Bay Minette, Alabama, April 28-May 7, 1978.

'.XVIII. Chemical Parameters Recorded by Test Aquarium During Flow-through
Acute Toxicity Testing Conducted at Reichold Chemicals, Inc.,

Newport Division, Bay Minette, Alabama, April 28-May 7, 1978.

XXIX. Survival Data Recorded by Test Container During Acute Toxicity

Testing Conducted at Gardinier, Inc., Tampa, Florida, June 17-21, 1978.

XXX. Chemical Parameters Recorded by Test Aquarium During	Flow-through

Acute Toxicity Testing Conducted at Gardinier, Inc.,	Tampa, Florida,
June 17-21, 1978.

XXXI. Survival Data Recorded by Test Aquarium During Acute	Toxicity Testing

Conducted at Pfizer, Inc., Southport, NC, July 8-12,	1978.

XXXII. Chemical Data Recorded by Test Aquarium During Flow-through Acute
Toxicity Testing Conducted at Pfizer, Inc., Southport, NC,

July 8-12, 1978.

iXXIII. Survival Data Recorded by Test Aquarium During Acute Toxicity Testing
at Diamond Shamrock - Chrome Chemicals Plant, Castle Hayne, NC,

July 12-19, 1978.

XXXIV. Chemical Parameters Recorded by Test Aquarium During Flow-through
Acute Toxicity Testing Conducted at Diamond Shamrock - Chrome
Chemicals Plant, Castle Hayne, NC, July 12-19, 1978.

-------
26

Table

//	Table

XXXV. Survival Data Collected by Test Aquarium During an Acute Toxicity
Study at the Brunswick Pulp and Paper Company, Brunswick, Georgia,
August 7-13, 1978.

XXXVI.

Chemical Parameters Recorded by Flow-through Test Aquarium During
an Acute Toxicity Study Conducted at the Brunswick Pulp and Paper
Company, Brunswick, Georgia, August 7-13, 1978.

-------
Table I.

BlcliT.lr.il H.it.i .ml Cliem I c.i I Pn r.n i" tor-; Kccot |. .1 I", leM Aqnirluin During .1 ri nw-Tlir'iifJi 96-h'.'iir Aoite roy|i-ll\ iMirl,1
Conducted /it Weslvnco Coipor.it !iin, ll.itl'i f: 11. i r los ton , SC. April 29 - Mnv 6f 197/

111 nnm

"~o "74

hr 11 r

o. of

=. S'lTV: .

10
10

10
10

10

10

10
10

10
JO

10
10

4 8
hr

10
in

10
10

10
10

10
10

10
10

10
10

•ng
96
JiL

10
10

10
10

10
10

10
10

10
10

10
10

No. of

Shrinn SurvjivlvQ	

0 24 43" 72" 91,
11 r 11 r hr lir 11 r

I' i ;m: 1 vi-d (i- ",;i:
(--/I)
0 24~~ 48 72
h v In hr hr

7.3

7.6

7.3
7.3

7.3

6.7

6.9
6.9

6.3
6.1

5.3

5.3

7.2
7 . 1

7.0
6. 9

6.9
6.7

6.6

6.5

5.9
5.(1

4.6
4. 7

6.4
6.3

5.9
5.8

5.7

5.5

5. 1
5. I

4.1
',.2

2.8

2.8

6.5
6. 1

r> .9
5.9

5.9
5.4

5.3
5.3

4.2

2.6
2.6

96
hr_

(,.6
6.6

6.0

6.0

5.9
5.8

5.1

5.3
S. 5

4.3

4.4

0

J._r_

7.6
7.6

7.6

7.6

7.5
/. 6

7.6
7.6

7.6
7.6

7.6

7.6

'TJ-
J1
7.6
7.6

7.5

7.6

7.5
7.5

7.5
7.5

pll

"48
hr

7.6
7.6

7.6
7.6

7.6
7.6

72
hr

7.8
7.R

7.7
7. 7

7. 7
7.7

7.5

7.5

9 6
h r

7 8
7.8

7. 7
7. 7

7.7
7. 7

7.5
7. 7

Tut.il A11-31 In 1 tv
(~~l* CnO?3)

48
hr

0

75
75

go
so

1 ?
9".

105
105

1 10
1 1"

1 75
1 75

106
102

172
1 7 J

ICS
I 10

198
19 5

72 | 96
hr I hr

201
203

70
70

8^
3 7

108
1 1 1

19'
i

0

_Ji_r	

16.5
16.5

15
1 5

1 5
1 5

14

14

1 2
1 2

C

8

J i r

16.'
16.

15.
15.

15
1 5

14
14

1 2

12

S
8

• I i -¦ i t v

I"
175.7

15.5

15.5
15.5

14.5
14.5

14
1 4

12

72

!' r

15.5
15.5

I'- 5
15.5

U
14

13
11

11
11

8
fl

96

-------
Table II.

Biological Data .nnd C!.c,Ic,I F-irorcteis Recorded by Test Aquarlu-a DUrlnZ a Flow-Throur.h 96-hour Acute Toxicity Stud/
Conducted jt Verona Dyestuff Division of Mobay Chenlcal Corporation, Charleston, ,C, Hay 23 29,





0. of

, s!

tlo. or

Dissolve.! Oxygen
(-/i)

pU

Total Alkalinity
(iWi C^'.n)

!>.¦• llni tv



0

24
hr

;s

hr

72

1 r

96
hr

0

24

48
hr

72
hr

96
iir

0
hr

24
hr

48
hr

72
hr

96

»i -

0
hr

*J4
hr

48
hr

72
It r

96
hr

0
lir

24
"¦r

48
hr

12
lir

9 6





1

l.r

ur

J b
t r

0.0
0.0

20
20

:o

20

19
IS

19 •
19

19
19

5

5

5

5

5

7.4

7.5

5.3
S.-5

7.6
7.4

8.3

8.4



7.6
7.6

7.6

7.7

7.7
7.7

7.7
7.7

7.8
7.3

80
79

81
81

76
78

75
75

75
75

16
16

16
16







5.6

5.6

20
20

20
20

20
19

19
18

17
16

5

5

4

4

4

6.8
6.8

7.7
7.7

7.4
7.2

7.9
8.1



7.6
7.5

7.6

7.7

7.7
7.7

7.7

7.6

7.7
7.7

77

77

80
78

75

76

76
75

76
76

16
16

16

16







10.0
10.0

20
20

2C
20

20
19

19.
17

i;
3

5

5

5

5

5

6.3
6.6

7.3
7.1

7.1
6.9

7.8
7.5



7.5

7.6









77
77









15
15

1'.
14







18.0
18.0

20
20

20
20

20
19

19
19

16
14

5

5

5

3

4

6.0
5.7

6.7
6.3

6.6
6.0

6.8
7.1



7.5
7.4

7.6
7.5

7.5
7.5

7.4
7.4

7.4
7.4

77
77

75

76

74
74

75
75

76
75

14
14

13
13







32.0
32.0

20
23

19

20

12
11

5
4

4
2

5

4

2

0

0

4.9
4.4

7.0
6.5

6.3
6.9

7.4
7.8



7.4
7.4









71

72









12
12

12
i:







0"- cy>

o o

20
20

7

0

6
0

1

0

1

0

2

2

0

0

0

2.3
4.1

7.7

7.4

6.3
6.8

6.8



7.4
7.3

7.3

7.4

7.4
7.4

7.4
7.4

7.4

7.5

69
68

69
71

71

72

74

75

74

75

10
10

9
9







100.0
100.0

14
14

C
0

0
.0

0
0

0
0

2

0

0

0

0

1.8
1.7

7.5
6.3







7.0
7.0









58
57

65
63







5
5

4
4







-------
Table III.

Biological rata nr.d Chemical Paroaotcs Recorded for Tost Aquariua During a Flou-ThrouCh 96^°ur Acute Toxicity Study
Conducted at the CI idr

96
hr

0
l.r

24
lir

48
hr

72

hr

96
hr

0
hr

24
hr

48
In

12
l-r

*0
ir

o o
o o

	J

10
10

9

9

7
7

7
6

5
5

10

10

9

10

9
9

8
7

8
7

6.3

6.4

f, 7
(..6

7.1

7.0

5.7
5.6

5.8

5.7

7.5

7.6

7.8
7.8

7.7
7.7

7.8
7.7

7.8
7.8

81

82

92
96

96
96

98
95

94

96

18
18

18
18

18
18

18
18

18

5.6
5.6

10
10

8
10

7

8

6
4

3

4

10
10

9
7

8
7

8
5

5
5

6.2
G.l

6.4
6.3

6.8

7.0

5.6
5.5

5.-4
5.6

7.6
7.6

7.9

7.9

7.8
7.8

7.7

7.7

7.8

7.8

85
85

96
94

98
93

95
93

104
98

17
17

17
IE

17
17

18
18

18
13

o o
o o

10
10

8

9

3

9

5
8

4
8

10
10

10
10

7

10

8
8

8
8

5.9
5.9

5.9
6.0

6.5
6.5

5.1

5.2

5.1

5.2

7.6
7.6









90
89









16
16

16
16

17
17

18
13

17
17

18.0
18.0

10
10

9

10

7
9

6
8

6

6

10
10

10
10

6
9

6

7

6

7

5.7

6.0

5.7
5.7

6.1

6.1

5.1

5.2

5.0
5.0

7.7

7.7

7.9

7.9

7.8
7.8

7.7
7.7

7.8
7.8

98

99

104
98

103
102

93

100

111

106

15
15

15
15

16
16

18
13

16
!6

32.0
32.0

10
10

9
9

9

0
u

9

6

5
4

8
8

7

8

6
8

6
6

6
6

5.6
5.4

5.4
5.2

5.3
5.0

5.0

4.9

4.4
4.2

7.8
7.8









108

108









13
13

13
13

13
13

16

17

14
2 A

56.0
56.0

10

10

10
9

8
7

7
6

3
5

6
u

5
fc

5
5

4

5

4
3

5.4
5.6

5.3
5.3

4.9
4.7

4.8
5.0

4.0

4.1

7.9
7.9

8.1
8.1

8.0
8.0

7.6
7.6

8.0

0.0

112
112

127

130

136
129

100
99

140
138

13
12

10
10

9

10

15
14

n

100.0
1U0.0





















6.2
5.4

5.7
5.7

5.3

5. C

4.6
!/..(>

5.4
3.9

8.4

8.4

8.5
8.5

8.3
3.3

8.4
8.4

8.5

8.3

165
173

158
170

167
176

179
185

185
185

1
1

2
2

1
1

2
2

3
3

-------
Table IV.

Survival Data Recorded for Wastewater Concentrations During
a Flow-Through Toxicity Study Conducted at Hercofina Corporation
Wilmington, North Carolina, July 20-24, 1977



No

of

sheepshead

No. of adult mysid

No. of young mysic

Concentration

minnows surviving

shrimp

surviving

shrimp surv.

- » —

of Wastewater

0

24

48

72

96

0

24

48

72

96

0

24

48

CO

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

0.0

10

9

9

8

8

10

10

10

10

10

10

10

10

0.0

10

10

7

5

5

10

10

10

10

10

—

—

—

5.3

10

10

10

10

10

10

9

9

9

9

10

10

10

5.3

10

10

10

10

10

10

10

10

10

10







9.6

10

10

10

10

6

10

9'

0

0

0

10

1

0

9.6

10

10

10

10

7

10

10

10

10

10







18.0

10

10

10

10

9

10

0

0

0

0

10

0

0

18.0

10

10

10

10

5

10

0

0

0

0







32.0

10

10

0

0

0

10

0

0

0

0

10

6

0

32.0

10

8

2

0

0

¦10

0

0

0 .

- 0

—

—

~~

56.0

10

0

0

0

0

10

0

0

«

0

0

10

0

0

56.0

10

0

0

0

0

10

0

0

0

0







-------
Table V.

Chemical Parameters Recorded for Wastewater Concentrations During a Flow-Through 96-hour
Acute Toxicity Study Conducted at HercoCina Corporation, Wilmington, TIC

July 20-24, 1977



Dissolved Oxygen





pll







Total Alkalinity



s.

1 I. ( 11 L I V



Concentration

(nig/1)

















(nig/1 CaC0 3)

•

(ng/niJ)



of is tew,iter

0

24

48

72

96

0

24

4 8

72

flfi

0

Ik

4S

72

96

P

24

48

72

96

U)

lir

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

h r

hr

hr

hr

hr

0.0



6.2

6.1

5.9

5.9

7.4

7.4

7.8

7.5

7.5

70

67

79

88

77

20

20

20

22

21

0.0



6.2

6.1

5.9

6.1

-

-

-

-

-

-

-

-

-

-

-

20

20

22

21

5.3



7.2

6.4

5.8

5.9

7.2

7.3

7.5

7.6

7.5

165

155

179

99

150

19

J 8

18

21

20

5. 3



6.8

6.2

5.7

5.9

-

-

-

-

-

-

-

-

-

-

-

19

18

21

20

9.6



6.9

6.4

6.0

5.9

7.2

7.2

7.5

7.5

7.5

195

183

185

102

182

18

1 8

19

22

20

9.6



6.4

6.3

5.9

5.7

-

-

-

-

-

-

-

-

-

-

-

18

19

21

19

18.0



6.7

6.2

5.7

5.7

7.2

7.3

7.4

7.6

7.6

331

301

310

144

352

16

17

J 7

21

18

18.0



6.3

6.2

5.5

5.8

-

-

-

-

-

-

-

-

-

-

-

17

18

21

18

32.0



6.4

6.5

5.0

5.8

7.2

7. 3

7.4

7.6

7.6

555

541

523

306

566

13

1 3

14

18

14

32.0



6.2

6.2

5.4

5.8

-

-

-

-

-

-

-

-

-

-

-

14

14

20

14

56.0



6.3

6.3

5.0

6.3

7.3

7.2

7.3

7.5

7.6

865

753

828

756

898

>10

10

12

13

10

6.0



6.3

5.9

5.1

6.0

-

-

-

-

-

-

-

-

-

-

-

10

1 L

13

10

100.0



7.8

6.6

4.7

7.1

7.5

7.6

7.6

7.7

7.7

1308

L208

1214

1406

1428

2

2

4

4

3

100.0



7.7

7.4

4.8

6.6

—

—

-

-

-

-

-

-

-

-

-

2

3

4

4

-------
VI.

Survival Data Recorded by Test Aqunriu	ng a FTow-Tlirough Acute Toxicity Study

Conducted at W. R. (..race & Co., Wl.l m i.ng (. on, NC
July 25 - Aupust 2, 19 77

No. of Mysid Shrimp Surviving

No. of Shecpshead Minnows Surviving

ConcenL ra-
ti on waste
water ('/,)

0.0
0.0

5.6
5.6

10.0
10.0

18.0
18.0

32.0
32.0

56.0
56.0

1

Adults (6-

8 mm)

¦k

Young

(1.5

- 2.0 mm)

Sub-

adults (1:

-20 nun)





Young

(A-7

*

mm)



1)

• 1 »



H

'J- 0

0

L! • i

' 1



¦>u



2'I

'(8

11

96



0

2-4

A 8

72

96

! : I"

i r

li r

hr



J""

lir

ii r

J.r

111

h i

lir

Jir

l.r

	1 it •_ _



J"'

Jir_

ill

_Jn'_

hL

10

9

8

8

8

3

3

3





6

6

6

6

6



10

8

7

2

0

.10

1

8

8 ¦

8

8











»
•











10

7

5

3

3

.10

8

2

1

0

3

0







6

6

6

6

6



10

10

10

9

9

10

8

2

0

























10

10

10

9

9

10

6

2

0

-

3

0







6

6

6

6

6



10

10

9

9

9

10

5

1

1

1





—

















10

10

10

10

10

10

3

0

_



3

0







6

6

6

6

5



10

9

A

A

2

10

A

0

—

























10

8

6

5

5

10

3

0

__

	

3

0







6

6

A

A

A



10

3

0

-

-

10

1

0

—

























10

6

1

0



10

0







3

0







6

0

-

-

-



10

1 0

-

-

-

10

1

0

—

























10

0



























¦







































1























* Results of this test have been included in this report in spite of unacceptably high control mortality,
since the pattern of mortality vs. concentration for all test concentrations is typical of mortality
tests in general. Results are to be interpreted wit11 caution.

-------
33

Table VII.

Survival Data Recorded During 48-hour Static Acute Toxicity Testing
Conducted at W. R. Grace Si Co. , Wilmington, 13C
July 25 - August 2, 1977

Concentration
of

Was teuater
(%)

0

1
3

10
30
60

No. of Mysid
Shrimp Adults
Surviving

0
hr

10

10

10

10

10

10

24
hr

10

10

10

10

48
hr

10

10

10

10

2

0

No. of Young Sheepshead
Minnows (5-10 mn)
Surviving;

0
hr

10

10

10

10

10

10

24
hr

10

10

10

10

10

10

48
hr

10

10

10

10

10

8

-------
34

Table VIII.

Ranges of Values for Chemical Parameters Monitored Periodically
for Each Wastewater Concentration Tested During a Flow-through
Acute Toxicity Study at W. R. Grace & Co., Wilmington, NC
July 25 - August 2, 19 77

Concentration of Wastewater (%)

Parameter	0	5.6	10	18	32	55

Dissolved	6.3-6.9 6.1-6.7 5.9-6.6 6.0-6.4 6.0-6.4 6.C-6.4

oxygen
(mg/1)

pH	7.7-8.1 7.8-8.1 7.8-8.1 -.7.9-8.2 8.1-8.3 8.2-3.5

Total	65-75	67-70	61-70	61-65	52-55	3?-u4

Alkalinity
(mg/1 CaC03)

Salinity	18-23	16-20	16-20	15-18	12-16	S-10

(mg/ml)

-------
Table IX.

Survival Data Recorded by Test Aquarium During an Acute Toxicity Study
Conducted at E. I. duPont de Nemours and Company, Wilmington, NC, August 2-6, 1977

Fresh-water Flow-Through Tests		Salt-water Static Tests

Concent rat ion
of Wastewater
(%)

	 		 —

No. of Ictalurus
punctatus surviving

^ _

No. of Daphnia

magna surviving

Concentration
of Wastewater
, (%)

No. of Mysidopsis
bah:a surviving

No. of Cyprinodon
varij^jjatus surviving

0

hr

24

hr

48
, hr

11
hr

%
hr

0
hr

24

hr

1

| Pi 00

hr

0
hr

24
hr

48
hr

hr

0
hr

24
hr

48
hr

72

0.0

10

10

10

10

10

10

10

10

10

0

15

15

15

15

10

10

10

10

0.0

10

10

10

10

10

10

10

10

10



















5.6

10

10

10

9

9

10

.10

10

10

5

15

14

14

13

10

10

10

10

5.6

10

10

10

10

10

10

10

10

10



















10.0

10

10

10

10

10

10

10

10

9

10

15

14

14

14

10

10

10

10

10.0

10

10

10

10

10

10

10

10

10



















18.0

10

10

10

10

9

10

10

10

10

20

15

15

15

14

10

10

10

10

18.0

10

10

10

10

10

10

10

10

10



















32.0

10

10

10

10

10

10

10

9

8

30

15

14

14

14

10

10

10

10

32.0

10

10

10

10

10

10

10

10

10



















56.0

10

10

10

10

10

10

10

10

9

50

15

15

15

15

10

10

10

10

56.0

10

10

10

10

10

10

10

9 •

9



















100.0

10

10

10

10

10

10

10

10

10



















100.0

10

10

10

10

10

7

7

7

7



















OJ

-------
iaDie a.

Chemical Monitoring Data Recorded by Test Aquarium During Flow-through Acute Toxicity Testing
<11 LI. I. duPont de Nemours and Company, Wi JinJ ngton, NC, August 2-6, 1977





Dissolved
( H'C /1)

Oxygen

pll

Total Alkalinity
(ing/1 CaC03)

Hardness
(nig/J)

%

WjsLewatcr

0
hr

24
lir

48
hi-

72
hr

96
hr

0
ilL-

24
lir

48
hr

72
hr

96
lir

0
hr

24
hr

48
hr

72
hr

96
hr

0
hr

24
hr

48
h r

72
hr

96
hr

0.0
0.0

7.1

7.2

6.8
6.8

6.8
6.7

1

6.4
6.3

6.3

6.4

7.2

7.5

7.5

7.5

7.6

33

31

35

39

38

40

38

38

—

38

5.6
5.6

7.2
7.1

6.6

6.7

6.6
6.4

6.2
6.1

6.1
6.0

7.7

7.6

7.8

7.9

—

63

43

72

75

—

41

40

36

—

40

10.0
10.0

7.1
7.1

6.6
6.6

6.3

6.4

6.1

6.2

6.0
6.0

7.7

7.6

7.8

7.9

7.9

63

40

73

75

74

41

39

37

—

38

18.0
J 8.0

7.2
7.1

6.6

6.7

6.4
6.2

6.0
6.0

6.0
6.0

8.0

7.8

8.0

8.0

—

101

62

115

121

—

41

40

37

—

40

32.0
32.0

7.1
7.1

6.5

6.6

5.9
6.1

6.0
5.9

5.8
5.8

8.1

8.1

8.2

8.3

8.2

164

101

187

204

195

40

39

36

—

37

56.0
56.0

7.1
7.1

6.1
6.3

5.9
5.8

5.7
5.7

5.8
5.8

8.2

8.1

8.3

8.4

—

231

125

279

294

—

40

40

36

—

38

100.0
100.0

7.2

7.3

6.3

6.4

5.4
6.2

6.5
6.5

6.5
6.4

8.3

8.6

8.5

8.6

8.5

366

371

379

457

463

38

30

32

	

39

u>

-------
Table XI.

Survival Data Cor Acute Toxicity Tests Conducted at
International Paper Company, Georgetown, SC, August 14-18, 1977

Flow-Through Tests

Static Tests

Concentration
of Wastewater
<%)

Minnows Surviving



Shrimp

Surviving

*

Concentration
of Wastewater

m

MLnnows
Surviving



Sh r i mp
Su rvi ving

0
hr

24
hr

S3*

00

72
hr

96
lir

0
lir

24
hr

4 8
lir

72

h

96
hr

0
lir

48
• hr



0
h r

48
lir

0

10

10

10

10

30



10

10

10

10

10

0

10

10



10

10

0

10

10

10

10

10



10

10

10

10

10













5.6

10

10

10

10

10



10

10

10

10

10

5

10

10



10

10

5.6

10

10

10

10

10



10

9

9

9

9













10.0

10

10

10

9

9



10

10

9

9

9

10

10

10



10

9

10.0

10

10

10

10

10



10

10

10

10

10













18.0

10

10

10

10

10



10

9

9

7

7

20

10

10



10

10

18.0

10

9

9

9

9



10

8

8

8

8













32.0

10

10

10

10

10



10

7

7

7

7

30

10

10



10

10

32.0

10

10

10

10

10



10

3

0

0

0













56.0

10

7

7

7

7



10

4

4

4

3

60

10

10



10

10

56.0

10

10

10

10

10



10

3

3

3

2













* Although the data from which these values were calculated presented a credible
pattern of mortality with respect to lethality concentration, this set of values
was not included in the industry report since a short-term drop in dissolved
oxygen in some aquaria may have prejudiced the results.

-------
XII.

CI i rut I i-.-i 1. r.t r.umu. 0

5.3

1.9

6.0

4.6

4.9

7.7

¦^J |
O I

1

7.9

oo :

i

7.7

130

152

148

152

162

'8'

• 7

7

8

8

56.0

5.3

2.2

6.3

4.7

5.2





















8

7

7

8

8

100.0

1.4

_

2.2

—

-

7. 7

7.7

i

! ^

i

7.7

7.7

L63

154

156

195

207

2

2

1

2

2

100.0

3.5



2.4

























2

2

1

2

2











































-------
39

Table XIII.

Biological Data Recorded by Test Aquarium During a Flow-Through 96-Hour Acute
Toxicity Study Conducted at the Cold Creek Plant of
Stauffer Chemical Company, Bucks, Alabama, September 11-15, 1977



No. of sheepsnead



'-M

O

o

adult

siysid



No.

of newlv

r.atchea



rcinnows surviving



shrinro

surviving





rr.vsid shrimp s

urvi"

/in

%

0

24

48

72

96



0

24

48

72

96



0

24

48

72



Was tevater

hr

hr

hr

hr

hr



hr

hr

hr

hr

hr



hr

hr

hr

hr



0.0

10

10

10

10

10



10

10

9

9

9



10

10

10

10



0.0

10

10

10

10

10



10

10

9

9

9'



—

—

—

—



5.6

10

10

10

10

10



10

10

9

8

3



10

10

9

8



5.6

10

10

10

10

10



10

10

9

4

2



—

—

—

—



10.0

10

10

10

10

10



10

10

7

3

0



10

10

7

6



10.0

10

10

10

10

10



10

10

8

0

0



—

—

—

—



18.0

10

10

10

10

10



10

10

1

0

0



10

10

6

5



18.0

10

10

10

10

10



10

¦ 9

4

0

0



—

—

—

—



32.0

10

10

10

10

10



10

2

0

0

0



10

3

3

0



32.0

10

10

10

10

10



10

3

0

0

0



—

—

—

—



56.0

10

10

9

9

9



10

0

0

0

0



10

1

0

0



56.0

10

10

10

10

10



10

1

0

0

0









—



-------
Table XIV.

Chemical Parameters Recorded by Test Aquarium During a Mow Through 96 hour Acute
Toxicity Study Conducted at the Cold Creek Plant of StauCfer Chemical Company, Bucks, Alabama, September 11-15, 19



Dissolved Oxygen





Pi I





Total

Alkalinity





Salinity







(mp/.l)













, Cmr;/1 as CaC03)





(inp/ml)





0

24

48

72

96

0

24

48

72

96

0

24

48

72

96



0

24

48

72

)6

Wastewater

llL"

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr



hr

hr

hr

hr

ir

0.0

8.6

8.4

7.2

7.0

7.4

8.1

8.0

8.1

8.0

8.0

71

71

72

71

71



18

16

18

18

L8

0.0

8.9

8.2

7.2

7.0

7.3

-

-

-

-

.i

-

—

—







—



"





5.6

8.7

8.0

7.0

6.9

7.1

8.1

8.0

8.0

7.9

8.0

71

70

71

70

71



16

15

17

17

16

5.6

8.7

8.0

7.0

6.9

7.0

-

-

-

—

—

—

—

-*



""







"





10.0

8.8

7.8

6.8

6.8

6.9

8.1

8.0

8.0

7.8

7.9

70

70

69

70

71



16

14

16

16

15

3 0.0

8.6

7.8

6.8

6.9

6.8

-

-

-

-

—

—

—











~







18.0

8.7

7.6

6.6

6.6

6.7

8.1

7.9

7.9

7.6

7.8

70

63

67

67

68



15

13

15

14

15

18.0

8.7

7.5

6.7

6.5

6.7

-

-

-

-

—

—

—









—

'



~

~

32.0

8.4

7.2

6.2

6.1

6.3

7.9

7.8

7.6

7.4

7.6

68

65

64

63

65



12

10

12

12

L2

32.0

8.4

7.3

6.3

6.1

6.5

-

-

-

-

—

"*











—

~







56.0

8.2

6.9

5.6

5.2

5.9

7.7

7.6

7.3

7.2

7.4

66

62

59

55

61



8

8

9

9

8

56.0

8.2

6.9

5.6

5.2

5.9

-

-

-

-

—

—

—

—



—













100.0

8.0

6.4

6.0

3.8

6.3

7.7

7.5

7.2

7.2

7.4

61

54

48

53

52



0

C

0

0

0

100.0

8.2

6.4

6.1

3.9

6.1

-

-

-

-

—

—

—

—

—







"

~

1



-------
Table XV.

Uio log ica I Data and Chemical Parameters Kecorded by tpst Aquaria DurLng a I" low-TIi rough
Conducted at the LeMoync PJant oC Stnuffcr Chemical Company, Axis,

September 16-20, 3977

96-hnur AcuLe To>:W.
A1nhama

it 11 y :'.t inly

head i nQ to























"Hssolvcl 0





1

m



P'I





I o ta 1

1.1 l.a

11n j L y

Concent rat i on



Surviving

Shrirro Surviving



(n"J I)

















fr-./f CiC02)



of Waste-

0



48

T>

9ti

0

24

48

72

96

0

24

48

72

96

0

24

48

72

96

0

24

48

i 2

96

water (%)



hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hT

hr

hr

hr

hr

hr

hr

hr

hr

hr

0.0

10

10

10

10

10

10

9

9

9

9

8.0

7.8

8.0

6.9

7.0

7.9

7.9

8. J

8.0

7.7

71

72

72

72

71

0.0

10

JO

JO

10

10

10

9

9

9

9

7.9

7.8

8.0

6.8

7.1

-

-



"

"











5.6

10

10

10

10

10

10

10

10

10

10

7.5

7.2

6.0

6.1

6.7

7.9

7.8

.

7.8

7.6

66

66

64

64

66

5.6

10

10

10

10

10

10

10

10

10

10

7.3

6.8

5.8

5. ;

6.6

-

-

-

—



"









10.0

10

10

JO

10

10

10

10

10

10

10

7.2

6.8

5.2

5.6

6.5

7.9

7.6

7.6

7.8

7.7

63

6 3

64

64

6 5

10.0

10

10

10

10

10

10

10

10

10

10

7.2

6. 7

5.2

5.4

6.5

-

~

~

"

"











lfi.O

10

10

10

10

10

10

10

10

10

10

7.1

6. 1

6.2

6.5

6.3

7.8

7.6

7.5

7.8

7.4

6 3

58

57

58

60

1 :i. o

10

JO

10

10

10

10

10

10

10

10

6. 3

5.9

6.2

6. !

6.4

-

"

-

""

"

"









32.0

JO

10

10

10

10

10

10

10

10

10

5.4

7.3

7.5

6.4

5.7

7.2

7.4

7.4

7.6

7.2

45

44

38

4 R

4 7

32.0

JO

10

10

10

10

10

10

10

10

10

5.3

7.5

-4. 5

6. 3

5.6

—

~

~



"











56.0

10

10

10

10

10

10

8

8

0

0

3.4

6.2

3.4

5.8

4. J

6.8

6.6

6. '

6.9

6.9

28

19

15

22

30

56.0

20

20

20

20

20

10

10

9

4

4

3.3

6.4

3.5

5.9

4. H

-

~

-

"

"

"









100. 0





















5.5

3.2

0.5

3.2

-

5.6

It.2

6.:'

3.6

4.1

3

-

12

-

-

1 00.0





















5.0

5.1

1.0

3.9























0
hr

17
17

16
l 6

16
16

I 5
1 5

1 2
I 2

10
1(1

1 8
18

If.
16

16
lf>

Hi
16

14
1 4

12
12

. i 1 i n f t y
f: r/ p: )

I r

15
15

14

14

14
14

1 4
14

1 2
12

15
15

14
14

14
14

14
1 4

12
12

8
R

3
3

-------
In	I.

liio loj, J c.il Data and Chemical I'a rame te rs Recorded by	Test Aquarium During	a Flow-through 96-hour Acute

le::irit.v Study (.'induct ed at dcToor te. re Ccrpn ra U i on ,	U i 1 :iti n [; ton , NC, November 7-11, 1977.

Was [ i: r li of Siieepshead Dissolved Oxygen	'I'oLal Alkalinity	Salinity

Concent ra- iluinovs _Sj ir v i v i i ij»	(mi: / 1)		Q[J		(nq/1. CaCO'j)			(pj) L )	

t ion """ (7Y-T 48 72 96 ~0 2 4 AH 72 96-	0 24 48 72 96	0 24 AH 72 96	0	24 48 72 96

(/_)	 hj lir h_r lrr In- h r_ h r br h r hr_ hr_ l_i r	li_r__ hr_ b r li_r_ lh_r	 br in	h r	lnr	li_r Jjjr hjr h_r

0.0 10 JO 10 10 10 6.8 7.6 6.5 8.6 7.7	7.9 8.0 8.1 8.0 7.9	152 152 153 136 132	16	15 U> 16 16

0.0 10 10 10 10 10 6.9 7.5 6.5 8.6 7.5	- - - - -	-----	16	16 16 16 15

5.6 10 10 10 10 10 7.0 6.7 6.7 8.0 7.4	7.9 fr.O 8.0 8.0 8.0	144 143 147 136 131	15	16 15 15 14

5.6 10 10 10 10 10 7.6 6.8 5.9 8.2 7.5	- - - - -	- - - - -	15	15 15 15 15

10.0 10 10 10 10 10 6.8 6.96.5 8.3 7.5	7.9 8.0 8.0 8.0 7.9	142 143 146 133 129	15	15 15 14 14

10.0 10 10 10 10 10 6.7 6.9 6.4 8.0 7.3	_____	1/,	\t, l/, 14 14

18.0 10 10 10 10 10 6.5 6.8 6.2 7.6 7.1	7.8 7.9 8.0 7.9 7.9	132 135 138 123 124	14	14 14 13 14

18.0 10 10 10 10 10 6.7 6.2 6.2 6.9 7.1	_____	11	13 ]4 13 14

32.0 10 10 10 10 10 6.6 6.3 6.2 7.9 7.6	7.8 7.9 7.9 7.9 7.9	117 126 124 109 115	11	12 12 11 12

32.0 10 10 10 10 10 6.5 6.3 6.4 7.6.7.4	-----	-----	11	12 12 10 12

56.0 10 10 10 10 10 6.4 6.6 6.0 6.9 6.6	7.7 7.6 7.7 7.7 7.7	95 97 96 90 100	10	8 9 8 8

56.0 10 10 10 10 10 6.2 6.4 6.1 7.4 7.4	-----10W888

1	1

100.0 * 6.2 5.9 4.0 5.3 4.9	7.3 7.5 7.2 7.2 7.1	37 3 3 36 38 31	0	0 0 0 0

100.0 6.7 5.9 5. 7 6.8 5.0	- - - - -	- - - - -	0	0 0 0 0

* Kstuarine or marine organises cannoL be Ler.ted in	undiluted, noiisaline	wastewater.

r-o

-------
Table XVII.

Biological Parameters Recorded for Wastewater Concentrations During a Flow-Through
Acute Toxicity Study Conducted at American Color and Chemical Corporation, Lobeco, SC

December 9-17, 1977

No. of £ypHnodcm va rl e partus, surviving	 	No. of mvsid shrimp surviving

%

I. 12

-15

day

old minnows

II. 26

-28

day

old minnows

I.

LblsJjJopJ5_i.1l baiLta

II.

-MvaW.QPJ

is. J

.¦«thia

ill.

mys 1 d

mixture

ste-

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Pac

Dec

Dec

Dec

Dec

Dec

Dec

Dec

Dec

De c

Dec

Dec

Dec

water

.12

13

14

15

16

13

14

15

16

17

12

' 13

14

15

16

13

14

15

16

17

14

15

16

17

0.0

10

10

10

• 10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

9

9

9

0.0

10

10

10

10

10

-

-

-

-

-

10

10

10

10

10

10

10

10

10

10

10

10

10

9

5.6

10

10

10

10

10

10

10

10

10

10

10

10

9

7

7

-

-

-

-

-

10

9

9

4

5.6

10

10

10

10

10

10

10

10

10

10

10

10

6

6

5

-

-

-

-

-

10

9

9

6

10.0

10

10

10

10

10

10

10

10

10

10

10

10

9

1

1

5

4

3

2

1

10

10

9

9

10.0

10

10

10

10

10

10

10

10

10

10

10

10

9

1

1

-

-

-

-

-

10

9

6

0

00

o

10

]0

10

10

10

10

10

10

10

10

10

10

5

0

0

5

4

0

0

0

10

4

0

0

00

o

10

10

10

10

10

10

10

10

10

10

10

10

6

0

0

-

-

-

-

-

10

8

7

4

.0

10

10

10

10

10

10

10

10

10

9

10

10

0

0

0

5

1

0

0

0

10

0

0

0

32.0

10

10

10

10

9

10

10

10

9

9

10

10

1

0

0

-

-

-

-

-

10

4

1

1

56.0

10

10

10

10

10

10

10

4

4

2

10

0

0

0

0

5

0

0

0

0

10

0

0

0

56.0

10

10

10

9

4

10

9

3

2

1

10

0

0

0

0

_

_

_

_

—

10

0

0

0

* A 70:30 mixture of Mysldopsis bigelowl and Neomysis americana was used in this test.

-------
Table XVIII.

Chemical Parameters Recorded for Wastewater Concentrations During a Flow-Through
Acute Toxicity Study Conducted at American Color and Chemical Corporation, Lobeco, SC

December 9-17, 1977

0.0
0.0
5.6
5.6
10.0
10.0
18.0
18.0
32.0
32.0
56.0
56.0
100.0
100.0

Dissolved Oxygen
(hir/1)

9.A
9.6

9.36.7

8.q

8.3

8.2

Dec l)ec
12 13

p)e
14

cjDccDec
15 16

7.97.41
8.Q7.4

7. C
6.46
6.4!5

,(¦

7.77.64.2
7.67.94.4

6.44.36.03.8
6.

4.15.23.7

74.:

4.25.83.1

7.66.35.6!4.36.3|4.1
7.06.45.

5. 8,4. 42.
6.15.24.

5.1
4.E
2.9
2.6

4.5

4.9(3.21

5

3.

O.tfO.21
0.30.2]

o.e

5.73.5

73.96.23.2
94.25.84.2
4|3.15.64.2
5.44.0

3.5

3.55.64.0

Dec
17

0.60.7

1.0

0.6

pH

Dec
12

7.9

7.9

Dec
13

8.28.C8.C7.98.0

8.28.C7.£7.87.8

7.98.28.07.97.87.8

7.98.38.28.C8.08.0

8.0

1.3

De
141

qDec
15

8.18.38.28.28.3

8.18.18.0

8.28.48.18.28.27.8

Dec.
16

Dec
17

8.1

.1

Total Alkalinity
(mft/1 CaCO^)

De c Dc cJ
12

147

19£

21 r

275294

381

512

866

148

197195

22421fJ

404

536

856

Dec Dec

14

14f

278

518

813

15

148

192

2.14

26C

397359360

48?

76C

Die
If

14S

181

214

265

485

Dec
17

Salinity
(mg/ml)

Dec

Dec

Dec

Dec

Dec

Dec

12

.13

14

15

16

17

18

1!

18

17

If

17

18

1!

18

If

16

17

18

If

If

If

If

16

18

If

If

If

If

17

18

15

18

17

If

17

18

If

1

If

16

16

18

15

17

16

16

16

18

15

17

17

16

16

18

If

1

16

If

15

18

15

1

16

If

16

17

1/.

If

16

If

16

17

14

If

16

If

1.5

16

14

If

14

14

14

15

11

15

14

14

14

Dec
12

16.0|

15.

15.

15.

15.

15.

15.
15.6
15.9
15.9

16.	C
16.1
15.3
15. (J

22.

9"21.9)2 2.4|22.2ll 7
822. (422
520.f
8121.?

Temperature
(°C)

Dec
13

822

• f

22. C

[22.2.

22.

22.

22.4

Dec
14

722.

522,

1J24

4|22. 2]17.7123

22.
23.5122.4117

521. 9(22 .2122
*>2.:
/J22

22.6|
22.
22.
122.
323.
23.0
23
23.4

Dec
15

822.
722.
] 22.
22.

(22.fr 18

22.£

Dec
16

17.

724.
723.

18.

2(18.

17.
2JL8.

18.
418.

18.
18.7

S 24.
C 23.

18.

Dec
17

.2
.9
.0
.7
.0
.7
.8
6

1123.9
123.8
1124.0
23.9
24.2
024.0

S 23.
123.

-------
Table "XIX.

Sitiviv.il 15;iL;i Recorded by To:; I Aquarium Durinj', .111 AciUe Toxicity Study Condnct¦ ¦ <. Aa 72 96 0 2A /.H 72 96 0 2A A3 72 96 0 2A	AT, 72	96

(/) br br hr hr hr b. lir lir lir br br In; br br br lir br	br lit' lir

10 JO 10 .10 10 10 10	10 10 10

10 JO 10 10 10 10 10	10 10	10

10 10 10 10 JO 10 JO 1.0 JO 10

10 JO 10 10 10 10 10 10 10 10

0.0

10

10

10

10

10

10

10

10

JO

1 0

0.0

10

JO

JO

9

9

JO

JO

10

.10

10

5.6

JO

JO.

10

10

8

JO

JO

10

10

10

5.6

10

JO

10

JO

JO

10

JO

10

10

JO

J 0.0

JO

JO

9

9

9

10

JO

10

10

10

J 0.0

JO

JO

10

10

10

10

.10

10

10

JO

J 8.0

10

JO

9

9

8

10

JO

10

10

10

J 8.0

JO

JO

10

10

10

10

JO

10

10

10

32.0

JO

JO

9

9

9

10

JO

10

10

10

32 .0

10

1)

10

10

10

10

10

10

10

10

56.0

10

7

7

7

5

10

JO

1.0

10

10

56.0

10

ft

7

6

6

10

10

10

10

10

Collection sites for wastewater used for toxiciLy testing:

1.	Just prior to spray irrigation - wastewater tapped from pump station between lioldinj; pond and r.praylield.

2.	From sprayfield drainage diteb leading to l$ij- Bof.jjy lironcb - wastewater was clipped Fr^m a br id(*.<"¦ crossing
tbe ditcb. on Olin property.

-------
Table XX.

(!Iu-iii i r.i I J'. i r.' iiu -1 i-rr, Kc-corili il In1 I1 11 u; -1 11 n >i i;11 M'r:;( A<| 'i, 11" I inn 1 • < 11 in:- .in Anil'- Tn..ii ily 'I 1111 y
Cond in: Lot! «11 tin- 01 in Corporal inn, !i:n|j-1 c:;:; I'owdcr Division, SL. M.iil::;, II., .J.inii.iiy I'j-I'i, 1978

l-.\r; I ( w.i Lor	Dissolved Oxygen	ToL.mI Alk.iliniLy	G.ilinity	Tempo r.i Lure;

fin.- ntrnljon	(rnf*/ I )	|)II	0"fj/J CaCO-j)	(ipf-/ nil)	(°C)

o :»/. /i8 72	%	o 26 /ip. /2 96 o 24 48 12 96	0 24	/.a	72 96	0	68 72	96

('/.)	Ijr	lir	lir lir	lir lir lir lir lir _ 11r	lir li_r_ Im-	lir_ li_r	I1'"	J,r _ l'r	It

0.0	9. 2 8.5 7.9 8.6	8.7	8.1 8.1 H.I 8.1 8.2 115 J 1.16 109 I.J J	16 15	15	20 20	12.3	20.1 18.5	18.2

0.0	9.3 8.'3 7.9 8.5	8.6	----------	16 15	15	20 20	11.3	18.6	20.1 17.7	18.1

5.6	9.3 7.0 7.A 8.A	8.5	8.1 8.1 '3.1 8.0 8.1 133 133 132 113 J12	14 14	17.	20 20	11.6	18.9	20.8 18.0	17.7

5.6	9.2 7.1 7.6 8.4	8.5	----------	15 14	16	20 20	11.3	19.0	20.7 18.0	17.8

JO.O	9.2 7 ..3 7 . 5 8.6	8.6	8.1 8.1 H.J 8.J 8.1 J 38 130 J 35 111 112	J6 16	16	20 20	11.6	19.2	21.6 18.2	17.4

10.0	9.0 7.2 7.6 8.3	8.3	----------	15 16	14	20 20	11.6	19.0	20.8 17.8	17.8

18.0	8.9 7.2 7.2 8.0	8.0	8.1 8.1 8.1 8.0 8.1 154 155 154 154 .138	14 13	12	18 1..	12.0	19.1	20.9 18.2	18.1

18.0	8.9 7.3 7.4 8.0	8.1	----------	13 13	13	18 18	J1.8	J9.6	21.5 18.8	17.7

32.0	8.6 7.0 6.6 7.6	7.3	8.2 8.2 8.1 8.0 8.0 191 191 J 89 190 196	11 12	11	15 15	12.8	19.8	21.2 18.8	13.6

32.0	8.6 7.1 6.4 7.6	7.2	- - - - - - - - - -	11 11	11	14 15	',12.8	19.8	21.3 19.0	18.3

56.0	8.3 6.8 5.4 6.6	6.3	8.3 8.2 8.1 8.0 8.0 252 251. 249 245 252	8 8	8	11 11	16.2	20.8	21.6 19.7	18.7

56.0	8.3 6.8 5.4 6.6	6.0	- - - - - - - - - -	8 8	8	10 10	16.3	20.5	21.5 19.6	18.6

100.0	8.3 6.1 4.2 5.9	6.0	8.5 8.6 8.4 8.4 8.5 364 366 367 365 367	13	3	4 2	16.0	18.2	19.0 16.7	18.6

-------
Table XXI.

Survival Data Recorded by Test Auuarium During
Acute Toxicity Testing Conducted at the Container Corporation
of America, Fornanthna ('.each, Florida
Fcbrunry 6-10r 1978







T1ow-throuqh

tests













Static To

51 ts_









c

icepshead





Mys id

Shrimp





Sheep

r.head

r*y

s i d

Shrinp







Mi n nows















Minnows











I. a r, tewa I or





































Con t en t ra tion

0

24

48

72

96

0

24

48

74

96

0

24

48

72

0

24

48

72

P)

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

n. n

10

10

10

10

10

10

10

10

10

10

10

10

10

10

4

4

4

4

0.0

10

10

10

10

10

10

10

10

10

10

















5. G

10

10

10

10

10

10

10

9

4

4

10

10

10

10

4

4

3

3

5.6

10

10

10

10

10

10

10

10

6

6

















10.0











10

10

10

4

4

10

10

10

10

4

4

4

4

10.0

10

10

9

1

1

10

10

9

1

1

















18.0

10

10

9

0

_

10

6

6

0

_

10

10

10

10

4

4

3

3

18.0

10

10

10

0

-

10

4

4

0

-

















32.0

10

10

0





10

2

0

_

_

10

10

10

10

4

4

4

4

32.0

10

10

9

0

-

10

0

-

-



















56 .0

10

0

_

_

-

10

0

_

_



10

10

10

1 U

4

3

2

2

56.0

10

0







1 0

0























-C-

-------
Table XXII.

Chemical Parameters Recorded by Test Aquarium During Flow-Through
Acute Toxicity Testing Conducted at the Container Corporation of America,
Fernandina Beach, rior ida, I'ebru.iry 6-10 1978



Dissolyed Oxygen
(mg/1 )

pll

Total Alkalinity
(mg/1 CaCOj)

So 1 i n i ty
(mg/ml)

Tempera ture
( °C>

Wastewater

0 24 48 72 96
hr lir hr hr hr

0 24 48 72 96-
hr hr hr hr hr

0 24 48 72 96
hr hr hr hr hr

0 24 48 72 96
hr hr hr hr hr

0 24 48 72 96
h r h r h r h r hr

o o
oo

7.9 8.7 8.6 8.3 7.1
7.9 8.7 8.7 8.3 7.1

7.7 7.8 7.9 7.8 7.9

113 113 107 84 115

13 16 16 16 18
13 16 16 16 18

13.0 12.4 16.7 18.8 19.3
12.7 12.0 15.0 18.0 18.7

5.6
5.6

10.0
10.0

l n. o

l n. o

7.7 8.2 6.6 5.5 7.0
7.6 7.7 6.2 5.0 7.0

7.5 7.6 7.6 7.4 7.9

130 134 125 105 125

12 15 15 14 16
12 15 15 14 16

13.2 12.5 15.6 ) 8 . n 19.0
12.8 12.3 15.1 1 f . 9 1^.0

7.6 8.2 7.6 5.2 7.8
6.4 8.0 7.6 5.7 7.0

7.4 7.6 7.6 7.3 7.9

135.137 128 105 125

12 15 15 12 16
12 1A 14 12 16

13.0 12.1 11.7 17.8 18.7
13.0 12.1 1-1.7 17.5 18.0

7.1 7.7 7.7 5.0 5.8
7.7 8.1 7.9 5.7 5.7

7.4 7.6 7.5 7.3 7.4

154 162 145 132 139

11 14 13 12 15
11 14 13 12 15

13.5 12.5 11.9 17..; 19.0
13.8 12.2 11.9 1 f.. 1 18.7

i

32.0
32.0

7.4 7.7 8.1 6.3 4.0
7.3 8.2 8.0 5.2 5.5

7.3 7.5 7.4 7.5 7.2

192 197 179 172 163

10 12 11 10 13
9 11 11 9 13

1 4 . f! 12.9 1 . 6 1 R . r, 19.2
1-1.7 12.7 15.7 ] B. 2 19.0

56.0
56.0

6.6 8.5 7.7 5.0 3.7
6.1 8.1 7.8 5.4 4.4

7.2 7.5 7.4 7.6 7.2

238 252 227 236 196

7 8 8 6 8
6 8 7 7 8

16.7 13.7 16.7 19.0 19.8
16.2 13.4 16.9 1 fi . 8 19.6

lon.o

6.9 9.7 8.1 6.5 5.4

7.4 7.6 7.6 7.2 7.1

379 361 339 302 235

0 0 0 0 0

23.9 18.4 22.9 2 6 . C 21.8

-is
Oc

-------
Table XXIII.

Sii i v i v.i I | \it . i ('o I I < r I > I I>y 'l i ¦ . I ij 11.11 j 11in l)i11 i iij>	.111 Ac 111 1 ¦	Ton I r I 1 v SI ml v . 11 t 11 < •

(''core,clown f> t.i; (¦ I ''o i pi 11.1 l iuii, )'ct.(u.,n , SO,	M; 111. 11 22-20, 1978

l.'.'M c-

F] ow-tliroui;h TcM s	Si.iUe i'jst/.

\i. 11 ¦. 'i

Minnow:;'' /-.live S11 r-i 1111 >A]ivc	Miunoi'.q*	Alive Si 1 rii:i|>-'c Alive

VQ Olir 241 1 r /i81ir 72hr %hr Ohr 24hr ^811r 72hr 96hr Ohr	?Alir	4JS1vr 721ic _96hr	Olir 2-Mir A811 r 7?hr 9f»_hr

JO	JO	10	.10 K)	10	l>	')

JO	10	10	10 JO	JO	10	JO

10	JO	10	10 10	JO	10	10

10	10	10	10 JO	10	10	JO

10	10	10	10 JO	10	9	9

10	10	10	10 10	JO	10	10

0.1)

]0

JO

10

JO

JO

10

10

10

10

10

0.0

JO

10

10

JO

10

10

10

10

10

10

b.(>

10

10

1(1

10

10

JO

10

10

10

.10

r).(>

JO

JO

10

10

JO

10

JO

10

.10

10

JO.O

JO

1 0

10

10

JO

JO

10

JO

JO

JO

JO.O

JO

JO

JO

10

JO

.10

10

10

JO

JO

1 f!. 0

JO

JO

10

10

JO

JO

JO

10

.10

JO

1 P. 0

JO

JO

JO

10

.10

JO

JO

JO

10

JO

'i

JO

10

JO

10

10

JO

JO

10

JO

JO

1)2.0

JO

JO

10

LO

10

JO

10

10

10

10

56.0

JO

JO

10

10

10

JO

10

8

7

7

5^.0

10

JO

10

10

10

10

10

10

9

9

* Cyprj nodon vari cpatus
i:y- Myr.i dopsi bahia

-------
Table XXIV.

I 'In ¦ n i < ¦. i I I', 11 .inn-1 c • :; I! cro ri led I j y I' I c n: -1 In < n i c.li T< ¦: '¦! A<|u.11 I um I >i 11 i n;\ .111 A < 111 i ¦ l'< > 11 i I y St 11 ¦ 1 v
CoikI ,ic Li; J nl the Gcorj;c town Steel. Corporal. i on , Ceorj'.otou'ii, SO, Mareli 22-20, I9/8

V.'.i'.Li u'iit.cr	Dissolved Oxygen	Total A I ka 1 in Lty	Salinity	Temperature

Ciuiccnt.rntion	p] 1	(mg/1 CaC03)	(1115;/in 1 )	(°C)

0 24 48 72 96	0	24	48 72 96	0 24 48 72 96	0 24 40 72	96	0 24 48 7 3 96

_(JO	 	11 r	11 l" _ 1 i_r	hr __hr_ lit	lir	lir Jir lir	 hr hr lir hr lir	hr hr hr hr	hr >jr hr lir lir hr

0.0	8. /( 8. .1 7. 7 8. 1 8.2	7.8	7.8	7.8 7.8 7.8	63 69 67 63 64	18 20 20 18	20 19.4 20.rj 22.1 20.4 21.0

0.0	8.6 8.4 8.0 8.3 8.3	-	-	- - -	_____	18 19 20 18	20 19.3 20.2 22.7 20.1 20.9

5.6	8.3 8.4 8.0 8.5 8.2	7.8	7.8	7.8 7.8 7.8	66 65 66 62 63	18 18 19 18	19	19.9 20.1 23.2 19.8 21.0

5.6	8.(> 8.5 8. J 8.4 8.3	-	-	- - -	_____	18 18 18 18	19	19.9 20.0 22.9 19.8 20.7

KJ.0	8.6 8.7 8.2 8.6 8.3	7.8	7.8	7.7 7.8 7.8	67 65 63 61 64	18 18 18 17	19	19.7 20.0 2 3.L 19. 7 20.9

LO.O	8.6 8.7 8.1 8.6 8.4	-	-	- - -	_____	]9 17 18 17	19	20.3 20.0 23.2 19.7 21.0

13.0	8.7 8.6 8.1 8.6 8.3	7.8	7.8	7.7 7.8 7.8	68 64 "62 59 63	17 16 17 16	19	20.1 20.3 23.0 19.9 20.7

18.0	8.9 9.1 8.1 8.6 8.4	-----	16 16 18 16	19	20.4 20.3 23.6 L9.8 21.0

32.0	9.0 8.8 8.1 8.6 8.3	7.7	7.8	7.7 7.8 7.8	69 63 61 57 63	14 13 15 13	19	20.6 20.7 23.4 20.1 20.8

32.0	8.9 8.8 7.9 8.6 8.4	-	-	- - -	_____	14 13 ij 13	t i)	20.6 20.7 23.1 20.1 21.0

56.0	8.8 8.5 7.7 8.4 7.7	7.7	7.7	7.6 7.7 7.7	71 59 55 55 60	10 10 10 10	19	20.7 21.1 23.1 20.8 21.0

56.0	8.7 8.6 7.8 8.2 8.0	-	-	- - -	-----	10 10 10 10	19	20.9 21.2 23.3 20.6 20.0

.100.0	8.7 8.6 8.3 8.3 7.6	7.6	7.4	7.5 7.6 7.3	' 72 54 45 53 44	0 0 0 0	I	22.9 22.0 23.8 20.2 19.0

100.0	8.4 8.3 8.3 8.1 7.1	-	-	- - -	_____	00001	23.8 22.6 22.7 20.3 19.6

-------
L:n r j L v.'i I. Pjl.i Ki'i:i'i ilr.l iiy ic:.t
Vil"{; i n l'I Uu'i i it I •; In: 01 |

1. !::l rr.i I li
C'ci IH.rll f. L'i.1 t i oil

	)_	

0.0

li . v>

13. i)

10.0
10.0
18. 0
US. 0
32. 0
VA. 0
3b. U
5().0

!¦ L i n; • L ll 1 U':.



0

:v»

AH

72

9 b

10

JO

JO

10

- 10

10

10

10

JO

JO

10

10

.10

JO

JO

10

10

10

10

10

10

10

10

io

JO

10

10

10

10

10

10

10

JO

] 0

JO

10

10

10

10

JO

10

10

10

10

JO

10

10

10

JO

JO

10

10

10

10

10

10

10

10

1')

10



Table

XXV.





















•'T

i ; r i M

1:11 r iii-,

11! i

T,

i c i L

I'i

:; t i i:;



II' 111 ( ( ( (1

.it



: r.

1' >¦'! >

. i,.|.

i lr,

A1 ..1 ¦

¦ ii.l'l

Ap r i 1

27

- 11 i y

1 ,

I 'J i







¦ ! :













S

L; i L i c

1 ¦ •:











r:,M,

1





.')ll(

11. ¦

i.l





::

y:i

.1





: lu i ::i

i'





1

;i i iiiio;.







£-11

r n:i

1'



:i h

<'i!>

7 2

9;>

0

:-!A

¦ifci

7 I _

_%	

0

:>>'>



7 2

y.

10

10

10

10

10

10

10

10

9

J 0

10

JO

10

10

n

J

9

y

'!





















10

10

10

J 0

10

10

JO

JO

10

10

10

10

10

10

rj

JO

10

10





















10

JO

H

a

10

10

10

J.0

10

10

10

10

10

i'j

10

10

lu

.10





















JO

J 0

1 U

V

10

10

10

10

10

10

10

10

10

L (J

10

10

! 0

10





















JO

10

10

10

10

JO

10

10

10

10

10

10

10

JO

JO

10

9

8





















JO

10

10

y

10

u>

10

10

10

10

10

i 0

10

10

10

J.O

')

H





















-------
Table XXVI.

th .in.,I	.. I i:. J '.j -)V:L	I'liiini- 1 1 o\» -1 h con;; 11 Acute 'tonicity Testing ConrUictcil ;i t

Vii,, i;: i. i ( lic.i i t n I s hum p ii.it r.l , Iljhilc, AI ih, !r.t,i Ap r i 1 27 - tiny 1, 1978

hi ...jiw.i d.^(,v u Toi.il Alkalinity	Salinity	Temper.ltum

OsLcr.i'n'r	_ t u- ' 1 ) 	pi! _ 	 	(i-i}'. / I CiCO^)	(mp,/nl)	(°C)

tiCcii L r.i t i;i u

u

1 * t

vi

/ 2

y u

u

24

AM

11

y'o "

a

~ 2/t

" 4 8

12

9 b

0

24

48

11

96

0

24

4 8

12

9

	(/; )	

111'

in'

i:i

'iu

in-

hr

lu-

In-

h,"

hr

In-

In"

In-

hr

lir

lie

!ir

hr

hr

hr

hr

hi-

hr

hr

1-.

0.0

/.;

/.y

9.0

7.1

6.y

7.4

7.4

7.6

7.0

7,o

00

60

60

oO

60

18

17

18

17

^ J

19.5

21.0

22.0

2 1.o

22

0. 0

'. 8

3

9.2

7. 4

7.2





















18

17

18

17

17

19.2

20.9

21.4

21.6

22

5. (.

'.0

u. y

9.5

7.3

a.o

7.6

7.5

7 • t>.

7.6

7.7

79

69

64

62

62

18

17

18

17

16

19.1

20.9

21.4

21.4

22

5.6

;;.o

y.o

9.6

7.5

8.1





















18

17

18

17

16

19.0

20.9

21.2

21.5

22

10.0

-

y. 3

y.y

7.6

8.2

7.7

7.6

7.6

7.5

7.7

96

7 7

/I

60

65

18

16

18

lb

16

-

21.5

21.5

21.5

22

10.0

8. 2

9.4

j . 6

7. b

8.1





















17

16

18

17

16

18.8

20.4

'1.2

21.3

22

18.0

l'.. (j

').(¦>

y.7

7.0

7.y

7.8

7.7

7.6

7.5

7.6

122

89

84

67

71

16

16

16

16

13

18.9

20.8

21.7

21.2

22

] h. (!

'.'.0

9.7

y. 7

/.9

7. a





















16

15

17

17

15

18.9

21.1

21.4

21..:

22

32.0

S

10.2

y.i

7.6

7.1

8.0

7.8

7.7

/. 6

7.6

112

93

106

80

78

14

14

16

16

14

19.1

21.0

21.7

21 .0

2 1

32.0

•;. y

10. 4

y. a

/. 7

7.2





















14

14

16

16

1 .

19.0

21.2

21.5

21.1

2 2

56.0

10. i

10.0

y.(j

5.7

7.4

8.3

U.J

8.0

7. a

7.8

'A'ib

ly.'i

17 3;

139

9 3

] 2

12

12

14

1 L

19.2

20.9

21.5

2l.ii

11

!) o. 0

i

1 o.H

10.0

/.I

;i. (,





















1 2

12

12

15

11

i J.l

21.3

21.7

21.1

2 I

lo'M)

i/

to. J

I (i. ij

11

y. :s

8. 6

8.y

8.6

8. /

8.2

3

6

h



r.

i <1 /.

1 1 /.

Tl /



> 2

Ln
NJ

-------
Tab±e xxvu.

Survival Mala Kecot iled by 'lest Aquarium Diirinj; Acute Toxicity Testing ConducLed at
Keicluild Mk.iiiic.iIk, Inc., NewporL Division, Bay HiaeLLe, Alabama April 2o - Hay 7, 1(J7H

!•' Low-llirou;',li Tests	StatLc Tests

a :. 1 1 i .a 1 e 1"





Mys id







SI lev

: 1»¦. I il

ad







My:; i d



Slice p:.head

ni." < - nl rat ion





'jlir imp







m

.niu A.

s







;l;r imp



m i minus

(')	

0

2 A

AH

; ""-J

1 n:

1

%

0

2't

A!',

72

yr»

0

24

AH 12 %

0

2 A

A .'j

72

0. 0

10



'J

y

y

10

10

10

10

10

10

10

¦•••- 10

10

10

10

• 10

0. (J

JO

y

y

y

y

JO

10

10

JO

10















'). G

10

10

JO

JO

J0

10

10

10

JO

JO

JO

10

* 10

10

10

10

10

j. ()

JO

u

li

a

8

10

10

10

10

JO















10.0

10

10

10

10

JO

JO

10

10

10

10

10

9

a

10

10

10

10

10.0

10

JO

10

10

y

10

10

JO

10

10















1 8.0

10

JO

10

10

J0

10

10

10

10

10

10

•k

•••¦• 10

10

10

10

10

18.0

JO

10

JO

y

u

10

10

10

10

10















J2.0

10

y

y

H

5

JO

10

10

10

10

10

*

7

10

10

10

10

32.0

10

10

9

i

5

10

10

JO

10

10















'je. 0

10

M

J

u

-

JO

10

10

10

JO

10

*

2

10

10

10

10

5(>. 0

10

]()

5

2

2

10

10

10.

10

10















Solutions were Loo turbid to permit accurate counting of orj;an i sins.

-------
Table XXVIII.

(lu „i u: ,i 1 I'.ii i i j K..> corded by Tc:.l Aquarium Darin;; IJow-Through Acute Toxicity Testing Conducted at
Keichokl (.I'.cm i c ;i 1 g , Inc. Newport Division, ISa y Ilincl.Le, Alabama April 28 - May 7, 1978

I) i :.',o I vi d ()>. y;; i'ii	Total A I I;.i I i ii i t y	Salinity	Tempera Lure

Wa . ; 11:i 1 ¦ ¦ r



(









I'11









/ 1 c,

iCO ,)





("if;/

'•I)







(°C)







Coiicl ii t i ,i t i oil

0

2 A

4 8

" ii

" 9 b

0

2 A

A 8"

'7 2"

9 b

____ _

2 A

A 8

if

9 b

0

2 A

A 8

72

96

0

2 4

4 d

72



•Jo

U)

III

\v:

hr

1 hr

hr

lu-

hr

ln-

hr

hr

hr

hr

t hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr



hr

0. 0

<>. 1

b. 9

(.. 8

b. 6

7. 9

7.9

7.8

7. /

/. 7

7.8

68

5 7

52

60

57

20

18

17

18

18

23. A

22.4

21.0

•i ¦>

u •

>

22.0

o.u

b. 1

b.. 5

b . b

b . 8

7.6





















20

18

17

18

18

23.3

22.7

21.5

) •>



22. 1

5. ()

6. 6

6.4

5.8

o. 6

7.1

7.8

7.7

7.5

7.6

7.6

70

62

55

62

60

20

17

16

17

16

23. A

22.6

2 1.7

22.

)

22.1

5. 6

b . 5

b.5

b J

6.6

7.5





















20

17

16

17

16

23.5

22.7

21.5

22.

3

22.1

10.0

6.9

b. J

b.4

6. 6

7.5

7.8

7.7

7.6

7.6

7.9

70

58

57

62

61

20

I 7

16

16

lb

23. A

2^.5

2 1.6

22.

>

22.0

10.0

6.7

b. 5

b . 2

(j . 6

6.8





















20

16

15

16

16

23.6

2 t. 8

21.8

2 2 .

3

22.1

J 8.0

6.5

b. 5

5.2

5.8

7. 4

7.7

7./

7.3

7.5

7.8

7 A

58

58

67

65

18

16

14

15

15

23.6

2 2.6

21.7

22.

3

22. C

18.0

b . A

6.4

4. 9

(>.9

7.6





















18

16

l'»

15

1A

23.6

2 2.6

21.5

•1 T

3

22 .0

32.0

6.3

b.2

5.1

4.9

7.6

7.5

7. /

7.3

7. A

7.8

82

69

65

73

73

I A

15

1 2

13

12

23.7

22.4

2 1.b

2 2 .

>

22 . 0

32.0

b. 0

0 . 5

J.5

5.0

7.4





















IA

1 5

12

13

12

2 '. 8

22.6

21.6

2 2 .

>

22 . (,

30. 0

5 . 2

5. 4

3.5

3.4

7.2

7. A

7.6

/ .2

7.3

7.8

9 2

78

78

83

8 J

10

10

8

9

8

2 A. 0

22.5

21.6



>

2 2 .

5b. 0

5 . J

5. b

4. 1

J. 6

7.3





















10

10

8

9

8

23.9

2 2 . 7

2 l.S

22.

I

21.9

100.0

J.J

0.4

0.5

0.2

0.2

/. 5

7.3

7.2

/. 3

7.3.

10A

1J 6

102

10 A

106

0

1

1

1

1

2 A. 0

2 2 . 7

22. 5

22 .

0

22. 1

100.0

2.4

0.4

0.6

0.2

0.2





















0

1

1

1

1

24.2

22.8

22.0

22.

0

22. i:

Ln
4>

-------
Table XXIX.

Suiviv.il U.iUi Recorded by Test Container Durinj; Acute Toxicity TesLing
Coiuliif; Led  s Li'w.i t e r
Co nc < n t. r ,i (. ion

	CO	

0

Ilys
24

if 00 1
id '.lir

/,H !

i in | >
12

90

Sliec
0

psl
24

i/001
l< '.ld
4 !i

111 i nnows
7.'. 90

//001 composite
Slice psliead in i nnows
0 24 4 8 12 90

If 005
Shoe pr.liond
0 24 4 8

ini nnou
72 90

v 000
Slice pshen d
0 2'i 48

-I i :mo
/.!

'is
')(

0.1)

10

10

10

10

It)

10

10

1 0

10

10

10

10

-f	

10

1 0

10

1 0

10

10

10

10

10

1 (J

If]

1 0

1 I

o.o

10

.10

10

10

10

1 0

JO

10

10

10

L0

JO

10

LO

10

10

10

10

10

10

10

10

10

10

K

5.0

10

10

10

10

10

10

10

JO

10

10

JO

10

10

10

10

10

10

10

10

10

10

10

10

10

11

5.6

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10





















10.0

J 0

JO

L0

10

10

L0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

I(

10.0

10

10

9

9

9

10

10

10

10

10

10

10

10

10

JO





















18.U

10

9

y

9

y

JO

J 0

1 0

L0

10

1 0

10

10

10

JO

10

10

10

10

10

10

I (J

10

10

1(

18.0

10

10

10

10

10

10

10

10

JO

JO

10

10

10

10

10





















J2.0

1 (J

1 0

10

JO

8

10

10

10

10

JO

1 0

10

10

10

10

10

10

10

10

10

10

10

10

10

I (

J2.0

10

10

10

10

8

JO

J 0

10

10

JO

10

10

10

10

10





















50.0

1 0

10

10

8

4

10

10

10

L0

JO

10

10

JO

10

10

10

10

10

10

10

10

10

10

10

K

50.0

1 0

y

y

8

1

JO

10

JO

10

10

10

JO

JO

10

1 0





















100.0

10

10

5

3

0

JO

10

JO

10

10

JO

10

JO

10

10





















100.0

10

y

6

4

0

10

10

10

10

10

10

10

10

10

10





















Ln
Ln

-------
Table XXX.

Clirin i r,i | I'fi r;iini! t. or s I >'< • f: o(I <_¦ < 1 by 'lest A'| 11.1 r i inn Diirinj', l' 1 ow-l.hrotij;h AcnLe
Toxi<;iLy TesLi ii;; Coiuhu; Lnl . 11 (i.i r 11 i n i u r , I in;., Tnmp.n, 1' I or i d.i, .Iimo 17-21, 1978.

I)i !'.soJ veil Oxygen	Tol.il Al l;;i I i 11 i Ly	S.i I. i n i. L y	Tempr r.i Line

W.isLi'v.'iLt'r	(mh/D	I'll	f'wj/l CaCO )	(mj',/ml)	(°C)

Dii'.'i'i' Lr.i I. i on 0 2A A8 72 96 0 24~A» /~2 96 (7~2A A8 72 96 0 2A~A8~72 96 0 24 ~48~_

Coiii.'cp Lr.i I. i on

0

2 A

A 8

7 2

96

0

2 A

A 8

/ 2.

96

0

2 A

A 8

72

96

0

2 A

A 8

72

96

0

2 A

A 8

72

96

{'/¦)

lir

lir

lir

lir

lir

lir

1i r

In-

hr

11 r

lir

hr

11 r

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

0.0

6. 5

j. 7

6. 5

6. A

6.9

8.3

8. 2

8.2

8.3

8.3

1 10

1 10

107

1 10

110

18

18

18

20

20

23.9

20.0

21.1

21.A

20.4

0.0

6. A

5.3

6.8

6.7

6.7





















18

19

18

20

20

23.5

19.9

21. L

21.1

20.5

5. 6

(). 6

5.9

7.2

7.0

7.1

8.2

8.1

8.2

8.3

8.3

113

11 A

11A

11A

112

20

20

19

20

20

23.0

19.8

21.0

21 .1

20.4

5.6

6.6

5.9

6.8

6.7

7.3





















20

20

20

20

20

22.9

19.9

21.2

21.2

20.4

I 0.0

6.3

A. 8

6.8

7.0

7.1

8.1

8.0

8.1

8.2

8.3

116

1 18

.1 18

I 17

J12

20

20

20

21

20

23.0

20.1

21.1

20.6

20. e

10. 0

6.6

5.7

7.1

6.7

7.3





















20

20

20

21

20

21.5

19.3

20.4

21.2

20.2

18.0

6. A

5. A

7.2

6.3

7.0

8.1

8.0

8. L

8.2

8.3

117

1L8

J18

120

112

20

21

20

21

20

22. 5

19.5

20.0

20. A

20. £

1 8. (J

6. A

5.5

6.3

6.9

6.9





















22

23

22

23

20

22.0

19.1

20. 2

20.4

20. A

32.0

6.3

5.7

6.7

6.6

7.2

7.8

7.7

7.8

7.9

8.3

131

133

13A

13A

113

23

23

23

24

20

22.3

18.8

20. 3

19.9

20. (

3 2.0

6.3

5.6

6. 6

6. A

7.1





















22

23

23

23

20

22.3

18.8

19. A

20. 6

20. C

5<>. 0

6.1

5.3

6. 7

6. A

7. A

7.6

7.5

7.6

7.7

8.2

J A 9

151

1 51

1 50

1 18

25

26

2 6

26

?J)

'23.5

19.1

20.1

19.9

20.1

5(>. 0

(. .0

5. A

6. 5

6. (j

7.2





















2f)

2 6

>u

2f.

2o

l. t

1 u. 9

19. J

2n. 'i

2().'

100.0

5. A

5.7

6. A

6.6

7.9

7.5

7. A

7.5

7.7

8.1

182

182

1 82

182

182

32

32

32

32

32

28.8

18.6

19.9

20.0

19.:

.100.0

5.3

6.0

6.9

7.1

7.8





















31

32

32

32

32

28. A

1 r,. 7

19.8

20.0

19.:

(_n

-------
Table XXXI.

Survival Data Recorded by Test Aquarium During Acute
Toxicity Testing Conducted at Pfizer, Inc., Southport,
NC, July 8-12, 1978

Flow-throtip.h tests	Static tests

Wastewater
concentration
(%)

0

Sheepshead

minnows
2 <4 48 72

96

0

Mysid
shrimp

24 48 72

96

0

Sheepshead

minnows
24 48 72

96

0.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

0.0

10

10

10

10

10





















5.6

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

5.6

10

10

10

10

10





















10.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10.0

10

10

10

10

10





















18.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

18.0

10

10

10

10

10





















32.0

10

10

10

10

10

10

10

10

10

9

10

10

10

10

10

32.0

10

10

10

10

10





















56.0

10

10

10

10

10

10

10

10

8

8

10

10

10

10

10

56.0

10

10

10

10

10





















Cn

-------
e XXXJI.







L'lici.i i f J 1 O.i t.i Kc
1 r ¦; L i lb; i.'o ml in' (. i

''•!) I'll L'll

¦ I .ii rt

liy Ti.';>L A<| n.i r i mn Our in;;
i zlT, 1 ii, S< hiL h|)o r L , 1

I' J nw-Llirou;',li
NC .hi1y 8-J 2 ,

A<-ute
L9 78

To x i r i t y







W.] > L l vs,i t u r



hi

1 1 Vi'tl U

y,;<

..¦ii



l"i



S.i
(

L i 11 i l y

Mil'./ Ill 1)





Tenipu i .i Lure

CO



o 110 c u 11 . t L ion

(/)

0
in

ll I'

"" 4 8
In-

/ 2

li i"

9(>~"
lu-

t j
i.i

2* >2 9
In' In' In' lu"

0
lu"

24
lir

48
lir

"7 2
In-

9 h
hr

0
lu"

24
lir

•'.8
lir

72
lir

9b
lu-

0.0

7.9

0. 0

o. b

ft.2

7.2

" o.i/1 7

.7 7.7 3.U 7.6

20

20

20

Hi

17

20.0

23.4

22.7

20.2

2 L.O

u. u

7.a

0 . (j

b. 6

8.2

7. J





2 (J

20

20

16

17

20.0

22. 5

22.7

19.0

21.0

5.0

K.J

7.1

7.0

8.3

7.2





19

19

19

16

16

20. 0

22.5

22.7

19.2

21.5

5. 6

a. o

b. 9

6.9

8.4

7.2





19

19

19

16

16

20.0

22.5

22.7

19.2

21. 3

10.0

7.9

b. 3

b. 3

7.9

6.7





J 8

18

18

15

15

20.0

22.2

22.8

19. 2

21.7

J o. u

7.9

b. 6

b. 3

7.8

b.7





J 8

J 8

18

15

15

20.0

22.2

22.8

19.2

21.4

18.0

7.9

b. '3

b.l

7. b

6. 7





16

J 6

16

14

14

20.0

22.2

22.8

19.1

21.5

16.0

7. 7

b. j

5.9

7. 1

b.5





16

16

16

14

14

20.0

22.1

22.8

19.3

21.5

32.0

/. 5

b. 2

5.8

6.9

6. 2





13

14

J 4

12

12

20.0

22.2

22.8

19.1

21.5

32. u

7.4

b. 3

3.9

/. L

6.4





13

14

14

12

12

20.0

22.2

22.8

19.3

21.4

36.0

7. o

o. 1

0.1

7.3

6.4





10

10

11

10

10

2U.0

22.3

22.8

19.6

21.0

3o. 0

7.3

0.0

b.l

7.9

6. 1





JO

10

11

1U

10

20.0

22. 5

22.8

19.8

21.0

100.u

7.4

7.0

b. 9

7.4

6.4

7.2'1 7

.7 7.8 8.0 7.7

4

3

4

2

3

J 9.5

22.0

22.4

22.7

20.8

1UD. (1

7.8

5. b

3.6

/. 5

6.9





4

3

4

2

3

20,0

22.2

22.4

22.5

20.5

3 [jII probe broken.

00

-------
Table XXXIII.

Survival Data Recorded by Test Aquarium During
Acute Toxicity Testing at Diamond- Shamrock - Chrome
Chemicals Plant, Castle Hayne, North Carolina
July 12-19, 1978

Flow-through Tests	Static Test

10-day-old	16-day-old 10-day-old

sheepshead minnows	sheepshead minnows sheepshead minnows

wastewater
concentration

(%) 0 24 48 72 96	0 24 48 72 96 0 24 48 72 96

0.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

0.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

5.6

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

5.6

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

18.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

18.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

32.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

32.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

56.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

56 .0

10

.10

10

10

10

10

10

10

10

10

10

10

10

10

10

100.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

100 .0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

10

-------
Table XXXIV.

Chemical Parameters Recorded by Test Aquorimn During Plow-Through
Acute Toxicity Testing Conducted At Diamond Shamrock - Chrane Chemicals
Plant, Castle Hayne, North Carolina, July 12-19, 1978





Dissolved Oxygen





Pi I





Total ALKaiinit.y





balinity





Temperature









(mg/1)

















(mg/1

CaCO-,







(mg/1









(°C)



wastewater



















































concentration



















































(%)

0

24

48

72

96

0

24

48

72

96

0

24

48

72

96

0

24

48

72

96

0

24

48

72

96



hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

hr

0.0

7.2

8.2

8.4

8.3

8.7

8.6

8.7

8.0

8.0

8.0

97

97

141

145

144

15

16

16

16

16

23.2

18.7

19.8

19.8

20.3

0.0

7.2

8.1

8.2

8.4

8.5





















15

16

16

16

16

23.2

18.8

19.8

19.9

20.2

5.6

7.2

8.2

8.3

8.5

B.6

8.6

8.7

8^0

8.0

8.0

98

95

139

143

139

14

16

16

16

16

23.0

18.3

19.5

19.7

20.1

5.6

7.1

8.2

8.3

8.2

8.6





















14

16

16

16

16

23.4

18.4

19.4

19.8

20.0

10.0

7.2

8.2

8.1

8.4

8.5

8.6

8.7

7.9

7.9

8.0

90

94

138

144

138

14

15

15

15

15

23.0

18.5

19.2

19.5

20.0

10.0

7.2

8.3

8.3

8.5

8.6





















14

15

15

15

15

23.5

18.4

19.5

19.8

20.0

18.0

7.2

8.2

8.2

8.5

8.5

8.6

8.6

7.9

8.0

8.0

85

99

131

140

134

13

15

14

14

14

23.1

18.5

1912

19.6

20.1

18.0

7.2

8.1

7.9

8.5

8.4





















13

15

14

14

14

23.5

18.5

19.3

19.9

20.0

32.0

7.2

8.1

7.6

8.2

8.2

8.5

8.6

7.9

8.0

8.0

76

81

120

152

128

12

14

13

14

13

23.1

18.4

19.2

19.8

20.1

32.0

7.2

8.0

7.3

8.2

8.1





















12

14

13

14

13

23.4

18.6

19.2

19.9

20.0

56.0

7.2

8.0

7.1

8.1

8.1

8.3

8.4

7.8

8.0

7.9

61

64

106

157

115

10

12

11

12

11

23.3

18.5

19.1

19.9

20.1

56.0

7.1

8.0

6.9

8.0

8.0





















10

11

11

12

11

23.3

18.7

19.2

20.1

20.1

100.0

7.4

8.5

7.7

8.0

8.3

7.9

7.5

7.6

8.2

7.9

21

25

48

184

86

6

7

8

6

8

24.3

18.0

19.6

21.4

19.9

100.0

7.3

8.5

7.3

8.0

8.7





















6

7

8

6

8

24.8

18.1

19.2

21.7

19.7

-------
Table XXXV.

MMVIVAL OA I A LOIltCHO Hy HSI AIHIAI-'11 "I I'lJi'irii. Atl ACUIL IOX1CJ1Y
STUDY AT Tut BUIlHiWlO HUM' AND CAl'M-* UlH>JANYt ill-'UilSt' I O • GKOHijIA

Mji.usr u/-ij. i'tr*

lit}*	[iii'OtlGH	U5TS		blATIC 111b IS ('





NO

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i'

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SIJHV I V 11|()





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96

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Hit

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Hit

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hi;

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10

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9

9

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10

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1 0

10

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10

a

6

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6

































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1 0

11)

10

7

1

7

7

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10





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7

































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11)

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111

10

8

H

8

8

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1 0

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1 0

1 0

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09

1 0

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10

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10

I 0

10.0

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1 0

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1 0

1 0

1 0

10

9

9

9

































1 . 0

1 u

1 0

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11)

1IJ

1 0

1 U

9

9

9

10

111

1 ii

1 0

1 0

10





10

10

11)

10

10

1 0

1 0

1 M

1". 1)

1 0

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1II

1II

10

7

7

7

7

































i'. <1

1 0

1 U

11)

1 0

1 0

1 0

H

b

b

b

1 »

1 (1

1 ()

1 ii

1II

1II





10

1 0

1 0

10

1 0

1 0

1 0

lj'>

ir . (1

1II

10

lo

1 0

1 0

10

9

7

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(j

































¦j'.. 1)

) 0

11>

1 u

1 0

1II

11)

V

H

H

fi

1 0

1 0

1 0

1 0

1 0

1 u





0 9

1 0

1 LI

1 0

1 0

1 0

1II

I' 1

V>. (J

111

10

11)

1 0

1 0

10

10

1 0

9

9

































I 'in . <|

1 (1

1 0

1II

1 0

1 (I











































1 IK). 0

h

11

It

(1

(>











































-------
Table XXXV1.

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18

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1 H. 1

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1 M . 0

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1 37

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7 . b

7. V



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137

1 3(1

16

1 6

1 6

lb

lb

Pi

2U. 7

2^.0

21 . u

1 H. J

id.O

KM

it . 0

C) . 0

J.9

b.9

7.7









1 bP









1 <.

l<.

14

14

14

Pi

PV.t)

P?..\

21.0

M.b

Jd . 0

'i • m

o . ?

b. t

• 7

't. ti

7.7









lb<.









1<<

14

14

14

14

PI

po. a

PP . 0

21 . U

1 (1 . 6

^ . 0

*i . (v

f>.b

L-. J

J. J

. 1

7. 7









1 7^









I 1

1 0

1 1

1 1

11

PI

 • v

?> •1 >

b • 1j

» b

4. j

7. /









1 !h









1 1

1 0

1 1

1 1

1 0

c 1

cfU . !

Pp. 1

20 . U

1 >1. I*

1 (J 0 . 0

*3. 1

b • '

D . 1



c

(.7

7.M

f. tt

7 * d



PPH



p u

P.ID

PP1

b

<.

5

5

<«

Pi

20. 1

PI. 1

20.2

O

lull. 0

. J

'j • '/

b • "D

b • u

^ . b

7.«

7.S

/•9

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» nil IM Kill ,.AI|K ,1,J| I li TO I.ujr IlLl-OWt NL A[) (,'jlib Wl Mi IAM.M.

-------