FOURTH QUARTER PROGRESS REPORT
FY 87
ram«SMi«sioN wcc^nu" r ¦» i ¦ m? - >a ««« »
ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
OFFICE OF RESEARCH AND DEVELOPMENT
U.S. ENVIRONMENTAL PROTECTION AGENCY
CINCINNATI, OHIO 45268
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.388J
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
ENVIRONMENTAL MONITORING AND SUPPORT LABORATORY
CINCINNATI. OHIO 45268
DATE: December 28, 1987
SUBJECT: FY87 Environmental Monitoring and Support Laboratory - Cincinnati
(EMSL-Cincinnati) Fourth Quarter Progress Report
FROM: Kathie L. Fieler, Program Analyst
Environmental Monitoring and Support
Laboratory - Cincinnati
TO: SEE ADDRESSEE LIST
Attached is a copy of the EMSL-Cincinnati fourth quarter progress report.
For your information, a few of the highlights of the various projects/activi-
ties this quarter include:
* A meeting was held during this quarter to plan for the revision/updating
of the manual "Microbiological Methods for Monitoring the Environment".
For details, please refer to Project 02.
* Details concerning the referee analyses, the quality control (QC) sample
program, performance evaluation (PE) samples, and the water pollution (WP)
studies are available in Project 04.
* Work continued during the-quarter to develop a general purpose liquid
chromatography/mass spectrometry method for volatile organic compounds in
water. Please refer to Project 05.
-* Further details concerning the three 304(h) notices to meet National Pollutant
Discharge Elimination System (NPDES) needs are outlined in Project 06.
* Annual Discharge Monitoring Report-Quality Assurance (DMR-QA) Study 7 was
completed. See Project 08 for details.
* A report entitled "Methods for Selected Synthetic Organic Compounds -
Summary Report" was completed. Further information is provided in Project
10.
* Project 12 provides details concerning the method validation studies con-
ducted by our Laboratory.
* Specifications were provided to the Office of Emergency and Remedial Response
for a format for inorganic data deliverables on computer readable media--see
Project 22.
If you have any questions, please give me a call at FTS 684-7304 or contact
the Project Officers directly.
Attachment (1):
As Stated
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ADDRESSEE LIST:
Office of Research and Development: Office of Water—Continued:
Vaun Newill (RD-672)
Erich Bretthauer (RD-672)
Curtis Harlin (RD-672)
Richard Frank (RD-674)
Brenda Washington (RD-674)
Michael Mastracci (RD-674)
H. Matthew Bills (RD-680)
Paul Palm (RD-680)
Elenora Karicher (RD-680)
Stanley Blacker (RD-680)
Gary Foley (RD-680)
Charles Brunot (RD-680)
Richard Nalesnik (RD-680)
Charles Plost (RD-680)
Vernon Laurie (RD-6800
John Koutsandreas (RD-680)
Michael Dellarco (RD-680)
David Graham (RD-680)
James Falco
Robert Snelling
A1fred El 1ison
Thomas Hauser
Francis Mayo
Calvin Lawrence
Frank Princiotta
Lester Grant
Steven Lutkenhoff
Rosemarie Russo
Clinton Hall
Thomas Murphy
Gilman Veith
Norbert Jaworski
Henry Enos
F. Gordon Hueter
Office of Water:
Rebecca Hanmer (WH-556)
Victoria Price (WH-556)
Joseph Cotruvo (WH-550)
Arthur Perler (WH-550D)
Craig Vogt (WH-550D)
Irwin Pomerantz (WH-550)
Maria Gomez-Taylor (WH-550)
Paul Berger (WH-550)
Arnold Kuzmack (WH-550)
Lowell Van Den Berg
Herbert Brass
Marian Ml ay (WH-550G)
Norbert Dee (WH-550G)
Tudor Davies (WH-556M)
Michael Quigley (WH-546)
James Elder (EN-335)
J. William Jordan (EN-338)
Martha Prothro (EN-336)
William Brandes (EN-336)
Geoffrey Grubbs (EN-336)
William Whittington (WH-551)
Edmund Notzon (WH-585)
James McDermott (WH-585)
Devereaux Barnes (WH-552)
Frederic Leutner (WH-553)
Timothy Stuart (WH-553)
Office of Solid Waste and
Emergency Response:
Henry Longest, III (WH-548)
Stephen Lingle (WH-548A)
Joan Barnes (WH-548A)
Marcia Wi11iams (WH-562)
Jeffery Denit (WH-562)
David Friedman (WH-562B)
Florence Richardson (WH-562B)
Office of Policy, Planning,
and Evaluation
William Garetz (PM-222A)
Robert Wolcott (PM-221)
Paul Friedman (PM-221)
Office of External Affairs:
Davi d Davi s (A-104)
R. Augustus Edwards (A-100EA)
Richard Laska (A-100EA)
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ADDRESSEE LIST--PAGE 2
Office of Administration and
Resources Management:
Samuel Bryant (PM-225)
Office of Regional Operations:
Ramona Trovato (A-101)
Regional Offices:
Michael Deland, Region 1,
Attention: Lester Sutton
Paul Keough, Region 1
Christopher Daggett, Region 2
Attention: Barbara Metzger
Wi11iam Muszynski, Region 2
James Seif, Region 3,
Attention: John Ruggero
Stanley Laskowski, Region 3
Lee DeHihns, Region 4
Attention: David Hopkins
Charles Sutfin, Region 4
Valdas Adamkus, Region 5
Attention: William Sanders, III
Frank Covington, Region 5
Robert Layton, Jr., Region 6
Attention: Robert Forrest
Frances Phillips, Region 6
Morris Kay, Region 7
Attention: Ralph Hazel
William Rice, Region 7
James Scherer, Region 8
Attention: Denis Nelson
Alexandra Smith, Region 8
Judith Ayres, Region 9
Attention: Arnold Den
John Wise, Region 9
Robbie Russell, Region 10
Attention: Carolyn Wilson
Ralph Bauer, Region 10
Edward Conley, Region 1
Barbara Metzger, Region 2
Greene Jones, Region 3
James Finger, Region 4
William Sanders, III, Region 5
Russell Rhoades, Region 6
Regional Offices--Continued:
John Wicklund, Region 7
James Lehr, Region 8
David Mowday, Region 9
Robert Courson, Region 10
Jack Gakstatter, Region 10
EMSL-Cincinnati:
Robert Booth
Thomas Clark
Terence Grady
Robert Graves
Daniel Bender
Teresa Fi restone-
John Teuschler
James Lichtenberg
James Longbottom
Gerald McKee
William Budde
Joseph Roesler
John Winter
Edward Berg
Harold Clements
Cornelius Weber
Robert Safferman
Robert Bordner
Wi11iam Horni ng
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Page _J of 65
CLIENT OFFICE 051
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
B101 WATER QUALITY
A WQBA/PERMITTING
03 REFERENCE BIOLOG. METHODS - WATER QUALITY
PPA (S)
RC
PROJECT
PROJECT OFFICER
METHODS/BIOLOGICAL
A EMSL CINN
01 REFERENCE BIOLOGICAL METHODS - WATER QUALITY
CORNELIUS I. WEBER
PHONE: 513-684-7337
******************************************
PLANNED START: 10/01/78 PLANNED END: 12/31/99
******************************************
PROJECT DESCRIPTION:
GOAL: Provide standardized biological tools for monitoring the toxicity of
fresh and marine waters and wasterwaters, for determining the effect of
pollutants on aquatic ecosystems, and to support inclusion of biological
methods in the regulatory processes.
RATIONALE: The project addresses Agency needs for scientifically valid and
legally defensible biological methods to support the Water Quality Based
Approach to NPDES permit limitations and provide information concerning the
quality of ambient Waters and wastewaters.
APPROACH: Work will continue to evaluate, standardize, compile, and publish
biological monitoring tools supportive of wasteload allocation, water quality
based NPDES permits, use-attainabiljty studies, and site-specific water quality
modifications. Methods for culturing and testing of freshwater and marine
organisms will be upgraded, standardized, and published. Methods will be
provided for monitoring water and wastewater toxicity to aquatic ecosystems,
for evaluating toxicity of sediments and sludges to freshwater and marine
organisms, for determining toxic components of complex effluents, and measure-
ments of threshold toxicity effects such as histological damage and enzyme
activity.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
Ruggedness testing of the Seven-day Fathead Minnow Larval Growth and Survival
Test continued. The mean weight (0.636 mg +/-15%) of larval fish fed twice on
day one was essentially the same (0.626 mg +/-13%) as for those larval fish fed
beginning on day two. The lowest observable effect concentration (L0EC) for
hexavalent chromium was the same for both feeding regimes. Larval fish fed
beginning on day three were statistically less sensitive, indicating that
feeding should begin at least on day two. Several side-by-side tests were
conducted with hexavalent chromium and three different age groups of larvae.
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Page _2 of 65
The age groups were 24-hours, 4-days, and 7-days old. The LOEC was 3.0 mg/L
using 24-hour and 4-day old larvae and 12 mg/L for the 7-day old larvae. Mean
weights for control fish were 0.839 mg +/-3%, 0.971 mg +/-12%, and 1.77 mg
+/-13% for the 24-hour, 4-day, and 7-day old, respectively. The data indicate
that the older larvae are less sensitive to hexavalent chromium than the
younger ones and suggest that the test may be initiated with larvae up to at
least 4-days old.
A series of seven, seven-day, sheepshead minnow larval growth and survival
tests, using copper sulfate and Forty Fathoms artificial sea salts, were
completed to determine the single laboratory precision of the method. The mean
weight for all the control larvae was 1.60 mg +/-12%. The LOECs for survival
and growth ranged from 100 to 200 ug/L and from 50 to 200 ug/L, respectively.
Nine paired 96-hour algal toxicity tests were conducted using Selenastrum
capricornutum and cadmium as a reference toxicant. Algal Growth Potential
Medium (AGPM) with ethylenediamine tetraacetate (EDTA) added was used in one
set of the tests and AGPM without EDTA was used in the other. Mean control
algal growth with AGPM plus EDTA was 1,262,565 +/-26% cells/mL and without EDTA
was 349,797 +/-56% cells/mL. The mean cadmium EC50 value with EDTA was 0.447
+/-53% ug/L, and without EDTA was 0.039 +/-79% ug/L. The data indicate that
addition of EDTA to the AGPM may significantly reduce the sensitivity of the
test with metals.
A draft final report, "Feasibility of Culturing and Testing the Mysid,
Mysidopsis bahia, Under Artificial Conditions", was received from Colorado State
University, Fort Collins, Colorado. The work was done under a cooperative
agreement.
The Journal of Water Pollution Control Federation Annual Literature Review of
"Effects of Pollution on Freshwater Organisms," prepared in a joint effort
with the Environmental Research Laboratory - Duluth, was published in
June, 1987.
STATUS OF LABORATORY OUTPUTS
B1861 "Report on Comparison of 8-Day Fathead Minnow Embryo-larval Survival and
Teratogenic Test and the 7-Day Larval Growth Test". Report completed
6/24/87.
B706 "Report on Laboratory Culture Techniques and Use of Two Species of
Myriophyllum, for Effluent Testing". Report completed 6/29/87.
B708 "Report on Rapid Tests for Biomonitoring Effects of Effluent in
Receiving Waters". Report completed 6/24/87.
STATUS AND SCHEDULE OF DELIVERABLES:
2036 DUE: 09/30/87 REVISED: COMPLETED: 09/28/87
MANUAL OF SHORT- TERM CHRONIC TOXICITY TESTS FOR EFFLUENTS AND SURFACE
WATERS FOR USE WITH MARINE ORGANISMS.
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Page 3 of 65
CLIENT OFFICE 051
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
B101. WATER QUALITY
A WQBA/PERMITTING
03 REFERENCE BIOLOG. METHODS - WATER QUALITY
PPA (S)
RC
PROJECT
PROJECT OFFICER
METHODS/BIOLOGICAL
A EMSL CINN
02 REFERENCE BIOLOGICAL METHODS-WATER QUALITY
CORNELIUS I. WEBER
PHONE: 513-684-7337
******************************************
PLANNED START: 10/01/78 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Provide standardized methods and QA procedures for microbiological
analysis of fresh and marine waters; wastewaters, sludges, amd sediment, and
for the detection of toxicity and mutagenicity in these media.
RATIONALE: This project addressess Agency needs for scientifically valid and
legally defensible microbiological data on water quality in support of. the
Clean Water Act,. Marine Protection and Sanctuaries Act, and Resource Conserva-
tion and Recovery Act.
APPROACH: Develop or modify, evaluate, standardize and publish methods for the
detection and enumeration of microorganisms in environmental samples, including
methods for enteric pathogens and indicator organisms in ambient water,
wastewaters and sludges. Microbial tests, such as the Ames test, will be
developed to measure toxicity and mutagenicity in wastewaters and sludges.
Rapid instrumentation methods will be evaluated to determine the effects of
toxicants on the natural aquatic biota by measuring changes in bacterial
densities, population composition and metabolic activity resulting from
environmental stress. Methods for detecting and identifying genetically
engineered microorganisms, and recently developed techniques (e.g. gene
hybidization, mono/polyclonal antibodies) will be evaluated.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
A proposal from New York University Medical Center for a cooperative agreement
to evaluate the Microscreen mutagenicity test was peer reviewed and is being
processed.
The report on the comparison of the hydrophobic-grid membrane filter (HGMF)
procedure and USEPA approved methods for coliform analysis of water was
published in the May, 1987 issue of the Journal of Applied and Environmental
Microbiology. The results indicated that recoveries of total and fecal
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Page 4_ of 65
coliforms by HGMF did not differ significantly from standard methods.
In the task to determine the relative efficiency of three effluent sample
preparation methods for the Ames Test, high performance liquid chromatograph
(HPLC) analyses and Ames Tests were completed on a nonmutagenic control
(reagent grade water prepared by reverse osmosis) and a known carcinogen
(para-nitrobenzoic acid) that had been processed through the three extraction
procedures - liquid-liquid (LL), resin-packed glass with gravity flow (GF), and
resin-packed stainless steel with applied pressure (AP). The HPLC results
indicated recoveries of 77.8%, 72%, and 58.2% for LL, GF and AP, respectively.
Results obtained with the Ames Test also indicated highest recoveries from LL
and lowest from.AP. The control showed no indication of contamination from the
processing systems.
The report, "Precision and Relative Accuracy of the Membrane Filter and Most
Probable Number with Total Coliform Methods with Several Water Types" was
completed. The heterotrophic plate count (HPC) method served as the reference
method for relative accuracy determinations. No difference was found between
the accuracy of the two coliform methods, the analysts, or analysis times. For
non-chlorinated effluents, the mean coefficients of variation for the membrane
filter (MF), most probable number, and HPC tests were 20.5%, 62.7%, and 23.3%,
respectively, while the values for chlorinated effluents were 40.7%, 70.3%, and
25.8%, and for groundwater samples were 43.6%, 86.5%, and 32.6%.
An interim report (2035A) was prepared on the development of methods for
identification of salmonellae from sludges, wastewaters, and leachates. This
report contains an extensive review of the published literature on recovery of
these organisms from the environment. Many variations of qualitative procedures
were found but there was only limited information on quantitative and rapid
noncultural methods. The report also describes inhouse feasibility studies of
potential MF media and filter aids.
An interim report containing a review of the literature and the project plan
was completed for the project to prepare samples for microbial analyses
and determine total populations in sludge.
EMSL-Cincinnati hosted a two-day meeting of Agency microbiologists to plan for
the revision and updating of the Agency manual, "Microbiological Methods for
Monitoring the Environment", held at the Andrew W. Breidenbach Environmental
Research Center. Section staff participated in a meeting of the American
Society for Testing and Materials, Committee D-14 on Water, and led Task Groups
on methods for total coliforms and general practice procedures for counting
colonies, glassware preparation, and sterilization activities.
STATUS OF LABORATORY OUTPUTS
B1317 "Report: Evaluation of the Microbial Test for Mutagenicity of Waste
Waters". Completed 1/29/87.
B1330 "Precision and Accuracy of Coliform Methods - Annual Report". Completed
6/17/87.
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Page 5 of 65
B719 "Updated Procedures for Total and Fecal Coliforms in USEPA
Microbiological Methods Manual". Deleted 3/9/87.
B800 DUE: 06/87. Report on Methods of Preparation of Sludge Samples
Bacterial Analyses". In revision in Section.
STATUS AND SCHEDULE OF DELIVERABLES:
2035 DUE: 06/30/87 REVISED: COMPLETED: 06/24/87
INTERIM REPORT ON METHODS FOR SALMONELLAE IN MUNICIPAL SLUDGES,WASTE-
WATERS AND tEACHATES.
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CLIENT OFFICE 052
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
B101 WATER QUALITY
A WQBA/PERMITTING
03 REFERENCE BIOLOG. METHODS - WATER QUALITY
PPA (S): METHODS/BIOLOGICAL
RC: A EMSL CINN
PROJECT: 03 REFERENCE pIOLOGICAL METHODS - WATER QUALITY
PROJECT OFFICER: CORNELIUS I. WEBER PHONE: 513-684-7337
******* *r* *********************************
PLANNED START: 10/01/78 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Provide standardized methodology for monitoring viruses in surface
waters, wastewaters, sediments and sewage sludges as mandated in the Water
Quality Act of 1987, the Marine Protection Research and Sanctuaries Act (PL
92-532), and the Resource Conservation and Recovery Act (PL 94-580).
RATIONALE: Address Agency's need for collection of scientifically valid and
legally defensible information concerning the quality of ambient waters and
wastewaters.
APPROACH: Develop methods or adapt existing methods for detection and
quantification of enteric viruses in environmental samples. Evaluate, standard-
ize, compile and publish methods for the collection of adequate and representa-
tive samples, concentration of viruses so as to obtain manageable volumes for
assay, detection of viruses as a consequence of infectivity, and identification
of viruses by serological typing. Develop procedures for mammalian
cytotoxicity and mutagenicity testing of environemntal samples. Conduct inter-
laboratory collaborative tests on existing and newly proposed virus mehtods.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
A chapter update to the USEPA MANUAL OF METHODS FOR VIROLOGY has been
distributed to those entered into the Manual mailing list. This update, Chapter
9, "Cell Culture Preparation and Maintenance", is the first of two replacement
chapters on cell culture procedures. The second replacement, Chapter 10, "Cell
Culture Procedures for Virus Assay", is currently undergoing peer review.
A revised draft has been received from Baylor College of Medicine for the
Cooperative Agreement CR811258 ("Improved Methods for Hepatitis A Virus and
Rotavirus Concentration and Detection in Recreational, Raw Potable and Finished
Waters").
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Page 7 of 65
Viruses isolated from New York Bight samples have been identified and a report
of the findings submitted to Region 2. Further support for their virus
surveillance program on ocean dumping in the New York Bight will be provided,
and the Region will expand the protocol for processing seawater to include
evaluation of virus monitoring methodology.
"Status Report on Single Laboratory Evaluation of Two Methods for Virus
Analysis of Soils" is in internal review.
The internal report, "Method for Mammalian Cell Toxicity Test", describing a
technique for detecting threshold cytotoxicity in water, has been rewritten to
incorporate the data from an internal document, "Status of in Vitro Mammalian
Cell Toxicity Test Procedures".
Viral testing has been completed on samples received from phase three of
studies by the Water Engineering Research Laboratory - Cincinnati
(WERL-Cincinnati) on retrofitted aerobic wastewater sludge digesters at the
Trumansburg, New York sewage treatment plant. Virus support provided for
studies by WERL-Cincinnati on single and two-phase anaerobic digesters at the
USEPA, Test and Evaluation Facility, is continuing.
A report assessing recovery efficiency of beef extract reagents for
concentrating viruses from municipal wastewater sludge solids has been
submitted to the Office of the Director for clearance.
Nine laboratories have submitted samples for single laboratory viral analyses
in conjunction with the American Society for Testing and Materials round-robin
testing of two methods for detecting virus in soil. Viral assays on half of
these soil samples have been completed. Assay of the remaining samples (organic
muck and clay) has been delayed pending resolution of cytotoxicity problems
encountered in their original assay.
The laboratory work on the first phase of a sensitivity and precision study of
virus plaque confirmation procedures has been completed. The compiled data
have been statistically analyzed and the results are being drafted into a
report.
Studies have been initiated for testing the applicability of serum substitutes
and extenders as alternative reagents for propagation of mammalian cell lines.
STATUS OF LABORATORY OUTPUTS
B721 "Report on the Optimization of Suspended Cell Culture Technique for
Enterovirus Monitoring of Water and Wastewater". Completed 6/24/87
B722 DUE: 3/87. "Method for Mammalian Cell Toxicity Test". In revision in
Section.
B723 "Report on the Status of Detection Methodology for Hepatitis A Virus and
Rotavirus". Completed 5/27/87.
B724 "Report on Improved Method for Virus Reconcentration by the Organic
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Page 8 of 65
Flocculation Procedure". Completed 6/29/87.
STATUS AND SCHEDULE OF DELIVERABLES:
2037 DUE: 12/30/86 REVISED: COMPLETED: 12/16/86
REPORT ON MORE COST-EFFECTIVE, IN-LINE INJECTION TECHNIQUES TO CONCEN-
TRATIVE VIRUS FROM AMBIENT WATER AND SLUDGE LEACHATES.
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Page 9 of 65
CLIENT OFFICE 051
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE
TITLE
BUDGET SUB-ACTIVITY
B101
WATER QUALITY
ISSUE
A
WQBA/PERMITTING
PPA (L)
02
PROVIDE QA SUPPORT PROGRAM OF WATER QUALITY
PPA (S)
QUALITY ASSURANCE
RC
A
EMSL CINN
PROJECT
04
PROVIDE QUALITY ASSURANCE SUPPORT FOR WATER QUALITY
PROJECT OFFICER
JOHN
WINTER PHONE: 513-684-7325
******************************************
PLANNED START: 09/01/77 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Assure USEPA that ambient water quality monitoring data generated either
by or for regulation setting, enforcement, or compliance purposes is scientif-
ically valid and legally defensible.
RATIONALE: This project is to maintain a sound database through use of
standardized sampling and analytical methodology, applied in an ongoing Quality
Assurance Program for all of USEPA's ambient monitoring programs.
APPROACH: This' project consists of those needs identified as necessary to
support a quality assurance program: quality assurance guidelines and manuals,
a quality control check sample program for chemicals and biological test
organisms, a Repository for Toxic and Hazardous Materials which furnishes
calibration standards, and semi-annual performance evaluation studies conducted
to evaluate laboratory performance. This national level quality assurance
program supports the intralaboratory and interlaboratory quality control
programs of USEPA Programs, USEPA contractor, and other federal, state and
local laboratories conducting ambient water monitoring in the United States.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati produces and distributes chemical and biological quality
control (QC) samples and certified calibration standards as quality assurance
(QA) support for of the Office of Water programs. Microbiological and
biological samples are provided inhouse and extramurally. The National Bureau
of Standards (NBS) establishes selected traceability tests for water quality
analytes in performance evaluation (PE) samples. Formal PE studies are conducted
semi-annually with 500/700 laboratories/study from USEPA, USEPA contractor,
state, and local agencies conducting ambient water monitoring. Computer
programming and data processing support are obtained through the Agency's ADP
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Page 10 of _65
contract with Computer Sciences Corporation. Project activities are on or ahead
of schedule.
ACCOMPLISHMENTS AND RESULTS TO DATE:
USEPA Repository for Toxic and Hazardous Materials (Harry Kolde): , The
Repository distributed 20,300 ampuls to 420 USEPA, USEPA contract, state, and
local laboratories in the third quarter.
Conduct Referee Analyses.(Harry Kolde): Third quarter analyses were: QC and PE
samples, 201 ordered, 93 completed. Surrogate solutions for USEPA Methods
624 and 625, 9 ordered, none completed. Future work: continue verification
analyses as needed.
QC Sample Program (Ray Wesselman/Gary Collins): 46,330 chemical QC samples were
distributed to USEPA, USEPA contract, state, and local laboratories. The
following samples were produced to replenish depleted inventories: 5900 sets
of oil and grease, 6200 sets of chlorinated hydrocarbon pesticides, and 5900
sets of polychlorinated biphenyls (PCBs) Aroclor 1260.
A total of 919 biological QC samples were shipped. These were: Algae for
Identification 119; simulated plankton, 26; chlorophyll, spectrophotometry and
fluorometric, 250; reference toxicants for bioassays-cadmium chloride, 95, and
sodium lauryl sulfate, 21. Bacterial samples distributed were: Escherichia
coli, 153; Enterobacter aerogenes, 80; Klebsiella pneumoniae, 44; Pseudomonas
aeruginosa, 60; and Streptococcus faecal is, 53. Samples 3 and 4, Algae for
Identification were prepared. Design of a reference toxicant (copper) was
completed. It is to be prepared in the fourth quarter. Design of a sample
reference food (Artemia cysts) for use in bioassays required by the National
Pollutant Discharge Elimination System (NPDES) was completed and purchase of
the cysts will be made in the fourth quarter if funding is available.
Preparation of PE Samples (Harold Clements): New PE samples produced for
future studies include trace metals (12,000 ampuls), cyanide (6,000 ampuls),
and toal phenolics (3,000 ampuls). Analyses are continuing for all WP019
analytes to verify stability. Thirty-four ampuls containing 76 analytes are
being prepared for Study 19.
Water Pollution (WP) PE Studies (Paul Britton): By April 27, 1987, the
reporting deadline for WP018, data were received from 513 laboratories, 81
percent of the 631 laboratories that received samples. A total of 35,758
analytical results were evaluated and 86.4 percent were within study acceptance
limits. Individual reports for each laboratory and relevant study summaries
were mailed to USEPA regional and program office personnel as scheduled on
June 5 for their further distribution to participants.
WP019 was announced as scheduled on June 29, 1987. Samples for this study will
be shipped on August 10 to laboratories nominated by USEPA regional and program
office personnel.
STATUS OF LABORATORY OUTPUTS:
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Page 11 of 65
Output 2150(B) Report on Traceability to NBS due 12/86 was completed. Output
2150[C] Annual Report on Referee Analytical Services due 12/86 was completed.
Output 2150[D] Design, Develop, and Prepare PE Samples for WP Studies due 12/86
was completed.
STATUS AND SCHEDULE OF DELIVERABLES:
2033 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
ANNUAL STATUS REPORT ON THE USEPA REPOSTIORY OF TOXIC AND HAZARDOUS MATERIAL
FOR AMBIENT MONITORING
2034 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT ON THE QUALTIY CONTROL SAMPLE PROGRAM FOR AMBIENT MONITOR-
ING.
2150 DUE: 09/30/87 REVISED: COMPLETED: 07/27/87
INDIVIDUAL AND SUMMARY REPORTS ON SEMI-ANNUAL WP PERFORMANCE
EVALUATION STUDIES 17 AND 18
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Page 12 of 65
CLIENT OFFICE 050
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
******************************************
PLANNED START: 10/01/77 PLANNED END: 12/01/99
ft***************************'*****'*********
B101
A
04
A
05
WATER QUALITY
WQBA/PERMITTING
REFERENCE CHEMICAL METHODS WATER AND SEDIMENT ANALYSIS
METHODS\CHEMICAL
EMSL CINN
REFERENCE CHEMICAL METHODS - WATER AND SEDIMENT ANALYSIS
J. J. LICHTENBERG
PHONE: 513-684-7306
PROJECT DESCRIPTION:
GOAL: Provide standardized analytical chemical methods for quantification of
pollutants or their metabolic products in water, wastewater, biological tissue
sediment and sludge.
RATIONALE: USEPA has the task of monitoring ambient water quality either di-
rectly or as the result of analyses performed by the regulatory community.
USEPA therefore requires comparability of the data generated in diverse
processes. Emphasis is to be given to short term event monitoring and frequency
of measurement in order to ascertain patterns and assess effects.
APPROACH: Collect, evaluate, standardize analytical methods for toxic mater-
ials sensitive to toxicologically significant (low) levels of contamination.
Publish methods which assist controlling the levels of toxicants to sub-
chronic levels in non-target organisms and enhance beneficial use of waters.
This includes measurement of toxic concentrations in water, sediment,
tissue and sludge.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
Under this project, a combination of in-house and extramural investigations
are conducted by the Inorganic Analyses, Organic Analyses, Advanced
Instrumentation, and Sampling and Field Measurements Sections of the Physical
and Chemical Methods Branch to develop, evaluate, and standardize methods for
measurement of toxic chemicals in water, sludge, sediment, and tissue. The
objective is to publish methods to assist in controlling the levels of
toxicants and enhance the beneficial uses of water.
IDENTIFICATION AND DETERMINATION OF METAL IONS AND THEIR ORGANOMETALLIC AND
METAL CHELATE COMPOUNDS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)--The
manuscript, "Determination of Mercury (II) and Organomercury Compounds by
Reversed-Phase Liquid Chromatography with Reductive Electrochemical Detection",
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Page 13 of 65
has gone through in-house review and has been submitted to THE ANALYST for
consideration for publication. It was also sent to Headquarters as an 1869[A]
deliverable in May.
The evaluation of the solid-phase extraction (SPE) of the mercury analytes from
aqueous solution is continuing. Otis Evans attended a "Metal Speciation
Workshop" in Jekyll Island, Georgia on May 20-22, 1987.
CHEMICAL METHOD FOR EXTRACTION OF INORGANIC POLLUTANTS FROM BIOLOGICAL TISSUE--
A Quality Assurance Newsletter article was prepared announcing the develop-
ment and availability of Method 200.11, "Determination of Metals in
Fish Tissue by Inductively Coupled Plasma - Atomic Emission Spectrometry".
DEVELOPMENT OF METHODS FOR NONVOLATILE HAZARDOUS SUBSTANCES NOT AMENABLE TO
GAS CHROMATOGRAPHY (GC)/MASS SPECTROMETRY (MS)--Work continued to develop a
general purpose liquid chromatography (LC)/mass spectrometry (MS) method for
nonvolatile organic compounds in water. With a liquid-liquid extraction
from water followed by LC/MS, the grand mean recovery of 35 compounds was 71%
with a mean relative standard deviation of 11%. With a solid phase extraction
from water followed by LC/MS, the grand mean recovery was 76% with a mean
relative standard deviation of 17%. Further work is needed to develop a useful
internal standard and to automate the method. More instrumental stability is
needed, especially in the gradient controller, and this is available in newer
model commercial interfaces. Region 6 provided samples from solvent reclaimer
wastes and work began to determine the applicability of analytical methods to
these wastes. Similar samples from textile dye/formulators wastes are being
sought. Technical and engineering support in the maintenance of instrumenta-
tion was provided to several Branches and Sections of the laboratory.
METHODS FOR RESOURCE CONSERVATION AND RECOVERY ACT COMPOUNDS IN WASTEWATER--
Studies were designed to determine the optimum extraction conditions for
recovery of Appendix IX compounds from water using continuous extraction. All
of the semi volatile Appendix IX compounds amenable to gas chromatography have
been examined for recovery under various extraction schemes. Preliminary
results of these tests revealed recoveries lower than expected. The problems in
the extraction procedures were identified and the affected tests are being
repeated. Matrix studies employing the optimum extraction conditions are
scheduled to begin in the fourth quarter.
DEVELOPMENT OF DESIGN CRITERIA FOR MECHANICAL CONSTRUCTION OF A FIBER
OPTIC CHEMICAL ANALYZER--The refractive indices of 13 strands of fiber optics
coated with synthetic organophilic cladding were measured and found to range
from 1.3650 to 1.5257. Any value less than 1.485 indicates a usable fiber.
Fibers were also made for Battelle which has constructed a sensor system.
Unfortunately, one of the fibers broke during installation and the project has
fallen behind schedule due to this problem and lack of funds. Additional funds
have since been approved so that they can complete the project.
STATUS OF LABORATORY OUTPUTS:
None for this quarter.
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Page 14 of 65
STATUS AND SCHEDULE OF DELIVERABLES:
2039 DUE: 09/30/87 REVISED: COMPLETED: 09/28/87
STATUS REPORT: ANALYTICAL METHODOLOGY APPLICABLE TO MONITORING RCRA
CONSTITUENTS IN POTW EFFLUENTS
2040 DUE: 11/30/87 REVISED: COMPLETED:
STATUS REPORT ON ANALYTICAL METHODS APPLICABLE TO CHEMICALS FROM
SOLVENT RECLAIMER WASTES
2041 DUE: 1-1/30/87 REVISED: COMPLETED:
STATUS REPORT ON ANALYTICAL METHODS APPLICABLE TO CHEMICALS FROM
TEXTILE DYE/FORMULATORS WASTES
2042 DUE: 10/20/87 REVISED: COMPLETED:
STATUS REPORT ON DESIGN AND EVALUATION ,0F FIBER OPTICS TOXIC
CHEMICAL ANALYZER
1320 DUE: 09/01/86 REVISED: 03/30/87 COMPLETED: 03/30/87
Chemical Methods for Extraction of Inorganic Pollutants from Biological
Tissue
1869 DUE: 05/30/87 REVISED: COMPLETED: 05/29/87
REPORT ON METHODS FOR DISTINGUISHING FORMS OF METALS
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CLIENT OFFICE 051
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
B101 WATER QUALITY
C WASTE WATER TREATMENT TECHNOLOGY
42 CORRECT DEFICIENCIES IN APPROVED ANALYTICAL METHODS
PPA (S)
RC
PROJECT
PROJECT OFFICER
CORRECT DEFICIENCIES
A EMSL CINN
06 CORRECTION OF DEFICIENCIES IN APPROVED ANALYTICAL METHODS
J. J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 10/01/80 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: To assure that analytical methods presently approved by the Admini-
strator for regulatory measurement of pollutants in industrial wastewater
are reliable in all wastewater matrices.
RATIONALE: When analytical methods are developed for measurement of pollut-
ants in industrial wastewaters, they are tested in the effluents of a limited
number of industrial categories. However, when approved for regulations, the
methods are intended for use in discharges from all required industrial cate-
gories. Regulatory use of such methods discloses analytical deficiencies
which could not possibly have been recognized in their development. The
regulatory credibility of such approved methods is maintained through research
which rapidly recognizes the defects and corrects them.
APPROACH: Deficiencies brought to the attention of EMSL-Cincinnati through
promulgated channels by NPDES permittees or others, will be addressed to
improve the method validity as required by regulation. The Agency will review
existing approaches for minimum detectable level (MDL) and develop MDLs for
methods which do not have MDL data.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
This project involves a combination of extramural and in-house investigations
conducted by the Inorganic Analyses, Organic Analyses, Advanced
Instrumentation, and Sampling and Field Measurements Sections of the Physical
and Chemical Methods Branch. This work is designed to correct reported
deficiencies in approved National Pollutant Discharge Elimination System
(NPDES) analytical methods for specific applications. Several projects are for
the evaluation and standardization of methods for hazardous and toxic chemicals,
including development of method detection limits (MDLs) for those analytes that
do not have published MDLs.
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Page 16 of _65^
FY 87 REPORT ON DEFICIENCIES AND REVISED METHODS--(Item #2047[A] and #2048[A])
Three 304(h) notices are being processed. Two, the Virginia Edison Power
Company (VEPCO) agreement and the nationwide approval of the direct current
plasma emission method, have undergone Red Border review and have received
Office of Management and Budget (OMB) approval. Red Border review comments are
being incorporated into these notices and they will be sent to the Federal
Register by June 30, 1987, for publication. The third notice is to establish
standard test procedures for: Toxicity Tests for Aquatic Organisms,
Enteroviruses in Sludge, Mutagens in Wastewater, and Viruses in Water. Work is
continuing to determine precision, accuracy, and MDL data for Fluoride Method
340.1, 340.2, and 340.3. The Start Action Request (SAR) has been forwarded,
Vaun Newill has signed the SAR, and it is being forwarded to the Steering
Committee.
METHODS FOR PESTICIDES IN WASTEWATER--Battelle has completed the review of the
National Pesticide Survey (NPS) and Part 455 Methods. The entire consolidated
list of pesticides has been received now and experiments with traditional
adsorption cleanup procedures (Florisil, alumina, silica gel) have begun to
establish elution profiles. For polar pesticides, new column packings such as
Extralut and reverse phase packings will be evaluated.
STANDARDIZE Gas Chromatography/Mass Spectrometry (GC/MS) METHODS FOR
SLUDGE--This project is designed to be a follow-up to the study in B101/PPA 04,
and will not be initiated until the fourth quarter. If delays occur on the
Status Report: Analytical Methodology Applicable to Monitoring Resource
Conservation and Recovery Act (RCRA) Constituents in Publicly-Owned Treatment
Works (POTW) Effluents, deliverable 2039[A], they will also affect this
project.
REPORT TO CONGRESS ON 304(h) METHODS--After a six-week delay finding a suitable
contract mechanism, the Rocky Mountain Analytical Laboratory Division of
Enseco, Incorporated, was tasked to prepare the chemical methods portion and
consolidated report for Section 518 of the Clean Water Act. Meetings were held
on June 17 and 25 to review the technical details and timing of the report.
FLOW MEASUREMENTS FOR THE UNDERGROUND INJECTION AND CONTROL (UIC) PROGRAM--
An Evaluation of the Doppler Flowmeter (Hydra) has begun. The Hydra was
selected for evaluation since it is a portable flowmeter that is claimed to
be insensitive to particles and air bubbles. Initial studies showed that the
apparatus had serious defects and it was returned to the factory for major
repairs.
ACCUMULATOR COLUMNS FOR SAMPLING WATERS--Work on this project has been delayed
due to reassignment of personnel on a technical assistance project for the
Drinking Water Research Program and also to help in planning of an instrument
evaluation program.
OPEN CHANNEL FLOW MEASUREMENTS--Preliminary plans have been made to transfer,
ship, and reassemble an open channel flow test stand from the National Bureau
of Standards (NBS). The test stand will be used to develop a quality assurance
program for open channel measurements.
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Page 17 of 65
ALTERNATE TEST PROCEDURE (ATP) ACCOMPLISHMENTS--During the third quarter, a
total of 18 requests for approval of ATPs were received. The following
summarizes the applications reviewed by this laboratory and the actions
recommended: Received National Pollutant Discharge Elimination System (NPDES):
Nationwide (7); Limited Use (11). Action Taken: Recommended Approval (1);
Recommended Disapproval (0); Not ATP (0); Requested Data (0); Pending (17).
STATUS OF LABORATORY OUTPUTS:
[B]/EMSL 930 DUE: 2/87 REVISED: 1/87 COMPLETED: 5/18/87
"2nd Quarterly Report-Equivalency Applications Received, Notice and
Comments Published in Federal Register, Final Notice Published in
Federal Register"
STATUS AND SCHEDULE OF DELIVERABLES:
2047 DUE: 11/30/87 REVISED: COMPLETED:
ANNUAL REPORT: ITEMIZING DEFICIENCIES IDENTIFIED (FY 87)
2048 DUE: 11/30/87 REVISED: COMPLETED:
ANNUAL REPORT: REVISED ANALYTICAL METHODS WITH DEFICIENCIES
REMOVED (FY 87)
1854 DUE: 11/30/86 REVISED: COMPLETED: 11/25/86
ANNUAL REPORT ITEMIZING DEFICIENCIES IDENTIFIED FY 86
1855 DUE: 11/30/86 REVISED: COMPLETED: 11/25/86
ANNUAL REPORT REVISED ANALYTICAL METHODS WITH DEFICIENCIES REMOVED
FY 86
1859 DUE: 11/30/86 REVISED: COMPLETED: 11/25/86
ANNUAL REPT - FED REGISTER NOTICES FOR EQUIVALENCY APPLICATIONS RECD
NOTICE AND COMMENTS PUB IN FED REGISTER - FINAL NOTICE IN FED REGIS
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Page 18 of 65
CLIENT OFFICE 050
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
****************************** ************
CODE
TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
B101 WATER QUALITY
C WASTE WATER TREATMENT TECHNOLOGY
40 QUALITY ASSURANCE AND SAMPLE REPOSITORY
PPA (S)
RC
PROJECT
PROJECT OFFICER
QA AND SAMPLE REPOS.
A EMSL CINN
07 QUALITY ASSURANCE AND SAMPLE REPOSITORY
JOHN WINTER
PHONE: 513-684-7325
******************************************
PLANNED START: 07/01/72 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Assure USEPA that all monitoring data which it requires is sufficiently
documented for quality to both support its primary data quality objective and
its secondary uses--i.e., that intercomparable data are obtained.
RATIONALE: Data which meet uniform data quality criteria are intercomparable
allowing comparison with similar data generated by different analysts and/or
at separate times. Knowledge of confidence levels associated with each data
set helps prevent misuse of the data as well as with the decision-making
process.
APPROACH: Provide quality control samples and calibration materials from which
analyst can establish and document data accuracy. Provide an opportunity for
analysts to document their performance semi-annually through the analysis of
unknown samples. Evaluate and revise data quality criteria and additional
standards as needed. Monitor/evaluate the adequacy of existing procedures for
maintaining the Agency's QA support program.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati is responsible for producing and distributing quality control
(QC) samples and calibration standards and conducting performance evaluation
(PE) studies for ambient monitoring and point source discharges to support the
quality assurance (QA) program of the Office of Water. The projects which are
conducted inhouse and extramurally are on or ahead of schedule.
ACCOMPLISHMENTS AND RESULTS TO DATE:
USEPA Repository for Toxic and Hazardous Materials (Harry Kolde): Distributed
20,400 ampuls to 420 USEPA, USEPA contract, state, and local laboratories.-
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Page 19 of 65
Three new compounds and five other volatile organic chemicals (VOCs) and
synthetic organic chemicals (SOCs) were ampuled.
Conduct Referee Analyses (Harry Kolde): Third quarter analyses were: spiking/
calibration solutions, 76 ordered, none completed and QC and PE samples, 201
ordered, 93 completed. Future work: continue verification analyses as needed.
Traceability to the National Bureau of Standards (NBS) (Ed Berg): A priority
list of PE and QC samples requiring NBS analyses was agreed upon. Analyses
have begun on selenium in fish samples. NBS will produce 3,000 sets of
polychlorinated biphenyl (PCB) congeners.
QC Sample Program (Ray Wesselman): In the third quarter, 46,330 QC samples
were distributed to USEPA, USEPA contract, state, and local laboratories.
The following samples were produced to replenish depleted inventories: 5900
sets of oil and grease, 5900 sets of PCBs and 6200 sets of chlorinated
hydrocarbon pesticides.
Water Pollution (WP) PE Studies (Paul Britton): By April 27, 1987, the
reporting deadline for WP018, data were received from 513 laboratories, 81
percent of the 613 laboratories that received samples. A total of 35,758
analytical results were evaluated and 86.4 percent were within study acceptance
limits. Individual reports for each labortory and relevant study summaries
were mailed to USEPA regional and program office personnel as scheduled on
June 5 for their further distribution to participants.
WP019 was announced as scheduled on June 29, 1987. Samples for this study will
be shipped on August 10 to laboratories nominated by USEPA regional and
program office personnel.
Prepare PE Samples (Harold Clements): New PE samples produced for future
studies include trace metals (12,000 ampuls), cyanide (6,000 ampuls), and total
phenolics (3,000 ampuls). Analyses are continuing for all WP019 analytes to
verify stability. Study 19 involves 34 ampuls covering 76 analytes.
Projection of Calibrant Requirement Necessary to Support Domestic Sewage Study,
Resource Conservation and Recovery Act (RCRA) Section 3013(A), Section 4C of
Consent Decree (John Winter): Contaminated soil, and industrial and municipal
sludge QC samples are being prepared. Presentations by Bill Whittington and A1
Rubin (Office of Water Regulations and Standards) were attended on June 17.
The analytical methods and QA requirements are being sent to EMSL-Cincinnati
for consideration of.calibrant QC needs.
STATUS OF LABORATORY OUTPUTS:
Output 2044[B] Annual Report on Traceability to NBS due 12/86 was completed.
Output 2044[C] Annual Report on Referee Analytical Services due 12/86 was
completed. Output 1850[B] Design, Develop, and Prepare PE Samples due 12/86 was
completed.
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Page 20 of 65
STATUS AND SCHEDULE OF DELIVERABLES:
2044 DUE: 09/30/87 REVISED: COMPLETED: 07/27/87
INDIVIDUAL AND SUMMARY REPORT OF SEMI-ANNUAL WP PERFORMANCE EVALUATION
STUDIES 17 AND 18
2045 DUE: 10/30/87 REVISED: COMPLETED:
PROJECTION OF QA/QC REQUIREMENT NECESSARY TO SUPPORT DOMESTIC SEWAGE
STUDY, RCRA SECTION (3018(A), SECTION 4C OF CONSENT DECREE
1851 DUE: 01/30/87 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT OF QA DISTRIBUTION OF RESPOSITORY
STANDARDS AND QC SAMPLES
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CLIENT OFFICE 051
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
B101 . WATER QUALITY
C WASTE WATER TREATMENT TECHNOLOGY
41 DISCHARGE MONITORING REPORT (DMR) QUAL. ASSURANCE SUPPORT
DMR QA SUPPORT
A EMSL CINN
08 DISCHARGE MONITORING REPORT (DMR) QUALITY ASSURANCE SUPPORT
PAUL BRITTON . PHONE: 513-684-7325
PLANNED START: 10/01/79 PLANNED END: 12/01/99
PROJECT DESCRIPTION:
GOAL: Measure the ability of discharger laboratories to generate good quality
DMR data submitted by the 8000 major industrial wastewater dischargers to show
compliance with their National Pollutant Discharge Elimination (NPDES) permits.
RATIONALE: The NPDES is one of the most extensive monitoring networks operated
by the Agency. Within this system, dischargers monitor themselves and are
required to immediately report any NPDES permit violations. Enforcement
actions initiated by reported self-monitoring data are legally and technically
equitable only if the data generated within the NPDES system are of known
quality and are intercomparable. The DMR QA program provides a cost-effective
mechanism by which this data quality is monitored.
APPROACH: Performance evaluation samples are prepared containing basic analytes
listed on the NPDES permits. Acceptance criteria are derived from analyses of
samples by 300 USEPA and state enforcement laboratories. Samples are
distributed to 8000 discharger laboratories. Results are used to evaluate
quality of self-monitored data. Quality control samples are provided on request
and other corrective actions are taken to bring poor performers back to
acceptable levels.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
Clerical administration and conduct of the Discharge Monitoring Report -
Quality Assurance (DMR-QA) studies are conducted by the Bionetics Corporation.
A five-person staff prepares, analyzes, and distributes samples; maintains
study records; and handles most direct contacts with study participants.
Computer programming and data processing support are administered by the QA
Branch through the Agency's ADP contract with Computer Sciences Corporation
(CSC). These activities are all on or ahead of schedule for the current study.
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
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Page 22 of 65
Inhouse activities include overall management of the project and direction of
the Bionetics and CSC efforts, statistical programs, computer operations, study
design, and policy; with direct responsibility for producing the final
laboratory performance evaluation (PE) reports for each study. These
activities are on or ahead of schedule for the current study.
ACCOMPLISHMENTS AND RESULTS TO DATE':
Annual DMR-QA Study 6 (Paul Britton): A memorandum was sent to James Elder,
Director, Office of Water Enforcement and Permits (OWEP) on November 16, 1986,
reporting completion of Study 6.
Annual DMR-QA Study 7 (Paul Britton): By the finaldeadline for receipt of
data effectively May 19, 1987, 6697 responses.had been received from NPDES
permittees designated as "major dischargers",'by USEPA's OWEP. This
constitutes an 89.3 percent response of the 7502 permittees that received
samples. To facilitate a rapid evaluation of performance by the regions or
states in high-priority situations, the true values and PE limits for the study
were sent to the designated regional and state DMR-QA Coordinators on May. 28,
1987. Because of the volume of data and reports involved, the first copy of
reports will be mailed to the regions by mid-July. By mid-August, the regions
will receive another copy and the state will receive two copies. Participants
will be mailed their copy two weeks after the state copies have been mailed.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2046 DUE: 09/30/87 REVISED: 11/30/86 COMPLETED: 11/12/86
ANNUAL REPORT TO OWEP OF DMR-QA PERFORMANCE EVALUATION STUDY
(FY 86)
2188 DUE: 11/30/87 REVISED: COMPLETED:
ANNUAL REPORT TO OWEP OF DMR-QA PERFORMANCE EVALUATION STUDY -- FY 87
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Page 23 of _65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
******************************************
PLANNED START: 09/01/85 PLANNED END: 12/30/99
* *****************************************
C104
E
72
A
09
DRINKING WATER
DRINKING WATER TECHNOLOGY
QUALITY CONTROL AND PERFORMANCE EVALUATION SAMPLES DW AND QA
QC PE SAMPLES DW/QA
EMSL CINN
QUALITY CONTROL AND PERFORMANCE EVALUATION SAMPLES
JOHN WINTER
PHONE: 008-684-7325
PROJECT DESCRIPTION:
GOAL: Provide Quality Assurance Support to the Drinking Water Program.
RATIONALE: Quality control samples provide an independent check on the
analyst's technique and application of the USEPA approved methodology. Cali-
bration materials provide verified source of calibration standards, and
performance evaluation samples provide the bases for judging USEPA, state,
and local laboratory capabilities to conduct the required analyses under the
certification program of the Drinking Water Regulations.
APPROACH: Design and prepare QC and PE samples and standards series inhouse and
on contract for all contaminants and monitoring analytes regulated under the
SDWA. Verify sample true values, stability and homogeneity through analyses by
referee laboratories, and by traceability to NBS. Conduct formal performance
evaluation studies and report results to regulating authorities and participant
laboratories.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS:
EMSL-Cincinnati is responsible for the production and distribution of quality
control (QC) samples, performance evaluation (PE) samples and calibration
standards and for conducting PE studies for certification of drinking water
(DW) laboratories. PE studies are conducted with inhouse and extramural
resources.
ACCOMPLISHMENTS AND RESULTS TO DATE:
QC Samples (Ray Wesselman): A total of 11,650 QC samples were distributed in
the third quarter to USEPA, USEPA contract, state, and local laboratories.
6400 sets of trihalomethanes were produced and verified to replenish depleted
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Page 24 of 65
stock.
Prepare PE Samples (Harold Clements): PE samples were produced for the
volatile organic chemicals (VOCs) in the next DW study (WS021). Ampul 1
contains the eight specific VOCs and Ampul 2 contained some of the regulated
and unregulated VOCs (1445 compounds) (3,000 ampuls). Residual free chlorine
and pesticide ampuls were also produced for upcoming WS021 (6,000 ampuls). Day
zero verification confirmed the sample designs and concentrations.
USEPA Repository for Toxic and Hazardous Materials (Harry Kolde): 20,400
ampuls were distributed to 420 USEPA, USEPA contract, state, and local
laboratories. The VOCs to be regulated are now available as standards. Three
new DW compounds were ampuled as analytical standards and five other VOCs and
synthetic organic chemicals (SOCs) were re-ampuled to replenish low stocks.
Traceability to the National Bureau of Standards (NBS) (Ed Berg): A priority
list of PE and QC samples requiring NBS analyses was agreed upon. Analyses
have begun on Water Supply (WS) VOCs, WS herbicides, WS trihalomethanes, and WS
chlorinated hydrocarbon pesticides.
Conduct Referee Analyses (Harry Kolde): Third quarter verification analyses
were as follows: (1) spiking/calibration solutions of DW compounds, 76
ordered, none completed-; (2) QC and PE samples, 1005 ordered, 242 completed;
and (3) neat surrogates for USEPA Methods 624 and 625, 9 ordered, none
completed. Future work - continue verification analyses, as needed.
STATUS OF LABORATORY OUTPUTS:
Output 1653[B] Annual Report oh Referee Analytical Services due 12/86 was
completed.
STATUS AND SCHEDULE OF DELIVERABLES:
1653 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT ON PRODUCTION AND DISTRIBUTION OF QC/PE SAMPLES
AND CALIBRATION STANDARDS
1654 DUE: 12/30/86 REVISED: COMPLETED: 12/22/86
REPORT ON TRACEABILITY TO NBS
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Page 25 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
******************************************
PLANNED START: 09/01/82 PLANNED END: 12/01/99
******************************************
C104
E
73
A
10
DRINKING WATER
DRINKING WATER TECHNOLOGY
METHODS VALIDATION- DRINKING WATER QUALITY ASSURANCE
METHOD VALID. DW/QA
EMSL CINN
METHODS VALIDATION - DRINKING WATER QUALITY ASSURANCE
J. J. LICHTENBERG
PHONE: 513-684-7306
PROJECT DESCRIPTION:
GOAL: The mandate for laboratory certification is expressed in the SDWA. The
objective of this program is to develop less expensive methods for which maxi-
mum contaminant levels have been set. The methods should contain accuracy
standards, and be able to produce reliable data which can be used to support
regulations promulgated by the Agency.
RATIONALE: Conduct methods validation studies for trihalomethanes, purgeables
and total .organic halogens. Produce quality control check samples to support
the methods validation program. Conduct GC/MS analysis for priority pollutants.
Improve analytical methods for organic, inorganic, and microbiological
analyses for drinking water. Improve sampling and holding procedures.
APPROACH: Conduct analyses of raw and finished drinking water using the vali-
dated methods. Evaluate alternate test procedures to meet requirements of the
SDWA. Technical reviews, external and/or internal reviews will be conducted
and coordinated to provide a technically sound recommendation on approval
or denial of the proposed alternate test procedures.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
This project is assigned in the Organic Analyses Section for the purpose of
developing and evaluating methods for synthetic organic chemicals (SOCs) and
volatile organic chemicals (VOCs). The Environmental Monitoring and Support
Laboratory - Cincinnati (EMSL-Cincinnati) is cooperating with the Technical
Support Division (TSD) to evaluate pesticide methods proposed for use in the
future groundwater survey. This work is carried out both in-house and under
contract.
METHODS FOR VOCS--Preservation of volatile organics in chlorinated tap water
can be affected by dechlorination with ascorbic acid or thiosulfate followed by
acidification to pH 2 with hydrochloric acid. Ascorbic acid is preferred for
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Page 26 of 65
gas chromatography/mass spectrometry (GC/MS) methods, as sulfur dioxide can be
formed by acidification of thiosulfate and interfere with determination of some
early eluting peaks. No similar interference is found in the non-MS methods.
Draft journal articles on the two capillary methods were completed. The GC/MS
paper is being revised and will be completed early next quarter; the
photoionization detection (PID)/Hall paper is being sent out for peer review.
Final editing is being completed on the drinking water methods prior to
typesetting by the Center for Environmental Information (CERI).
METHODS FOR S0Cs--(Item #2060[A]) Under the Safe Drinking Water Act
(SDWA) Amendments of 1986, regulations call for some specific SOCs for which
suitable analytical methods are not available. These include endothall and
diquat. The present method of choice for diquat (and paraquat) is a high-
performance liquid chromatographic (HPLC) technique with ultraviolet (UV)
photometric detection and confirmation with a photodiode array detector.
Liaison has been established with personnel in the State of South Carolina and
other locations where similar approaches are being employed. A trip has been
scheduled next quarter to South Carolina to observe a real application and the
subsequent sampling and analyses. The project was initiated on derivatization
of endothall with pentafluorobenzyl bromide for subsequent gas chromatographic
analysis. Because of the hazardous nature of this reactant, present plans are
to examine alternative derivatization techniques for HPLC.
METHODS FOR DISINFECTION BY-PRODUCTS--An interim procedure was previously
provided for the haloacetic acids, chloral hydrate and 1,1,1-trichloropropanone.
Application of the approach to real world samples has shown that this technique
needs more refinement before it can be considered for routine application. A
micro-extraction procedure with methyl-t-butyl ether has been examined for the
dihaloacetonitriles, chloropicrin, chloral hydrate, and 1,1,1-trichloropropan-
one. The preliminary results look promising in terms of recoveries and detection
limits. These two methods cover most of the higher priority disinfection
by-products.
NATIONAL PESTICIDE SURVEY (NPS)-- The EMSL-Cincinnati is participating in the
NPS as a referee for Battelle on the analysis of phenoxyacid herbicides in
groundwater. Battelle's Method 3 is-a modified version of the original
EMSL-Cincinnati procedure. Calibration standards were exchanged with Battelle
to ensure that all field mesurements are related to a common basis.
A total of 8 field samples have been analyzed, 5 of which were also spiked
samples. The data were reviewed during the June 8-10 NPS meeting with
representatives from Battelle, Bay St. Louis, EMSL-Cincinnati, and TSD. As a
result of the meeting, a number of changes were recommended in the application
of Method 3, the major change being the accurate measurement of pH before
extractions. Future samples will be analyzed with additional quality control
including laboratory control standards, quantitation on both the primary and
confirmation column, and perhaps the use of weighed standards for some of the
analytes.
TECHNICAL ASSISTANCE TO DRINKING WATER RESEARCH PROGRAM--Modification of
automatic capping mechanism in an automatic sampler to be used for sampling
volatile organics from a force main of drinking water distribution system. An
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Page 27 of 65
American Sigma sampler has been modified with an EMSL-Cincinnati patented VOC
sampling device and the unit is now undergoing laboratory evaluation. Pre-
liminary indications are that the unit is working satisfactorily in sampling
VOCs automatically.
STATUS OF LABORATORY OUTPUTS:
None for this quarter.
STATUS AND SCHEDULE OF DELIVERABLES:
2060 DUE: 09/30/87 REVISED: COMPLETED: 09/28/87
METHODS FOR SELECTED SYNTHETIC ORGANIC COMPOUNDS - SUMMARY REPORT
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Page 28 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
C104 DRINKING WATER
E DRINKING WATER TECHNOLOGY
73 METHODS VALIDATION- DRINKING WATER QUALITY ASSURANCE
PPA (S)
RC
PROJECT
PROJECT OFFICER
METHOD VALID. DW/QA
A EMSL CINN
11 METHODS VALIDATION --DRINKING WATER QUALITY ASSURANCE
TERRY GRADY
PHONE: 513-684-7301
ic'kicie'kicie'k'k'k'kie'kie'k'kic'k'kitieic'kic'kie'kicieic'k'kicie'k'kic'k'k'kieie
PLANNED START: 10/01/86 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: The Equivalency Program encourages analytical methods development and/
or improvement by the regulated community and by manufacturers of pollution
measuring instruments. These alternate test procedures (ATPs) may be approved
for case-by-.case or nationwide use."
RATIONALE: 40 CFR Part 141.27 requires that the Agency consider and approve
use of appropriate ATPs for the NIPDWR.
APPROACH: After receipt of unsolicited ATP applications, necessary internal
and/or external technical reviews are conducted and coordinated to provide a
technically sound recommendation of approval or denial of the proposed ATP.
Items considered during review include, but are not limited to: availability
of a well documented test procedure; documentation of the applicability of the
proposed procedure; performance characteristics such as accuracy, precision,
and method detection limits; equivalency data to an approved test procedure.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
During the fourth quarter of FY87, six requests for approval of alternate test
procedures (ATPs) were received and processed. Two of the requests were
inquiries by LaMotte Chemical Products, Incorporated, concerning nationwide use
of test kits for free and total chlorine. Recommendations are pending for both
requests. The remaining four requests were for limited approvals. The City of
Chicago requested and received a recommendation for approval of a modified
extraction procedure for total trihalomethanes (TTHMs). The State of
Connecticut has requested approval to use high performance liquid
chromatography (HPLC) for determination of herbicides. The request is under
review. The U. S. Environmental Protection Agency (USEPA), Region 6, has
requested comments on a two-step titrimetric test procedure for total chlorine
and residual oxidants. Additional information is being forwarded so that the
review may be completed. CSMRI Analytica has requested limited approval to
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Page 29 of 65
proceed with the use of inductively coupled argon plasma (ICAP) spectrometry,
although final nationwide approval is expected soon. A recommendation for
approval has been made.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2057 DUE: 12/30/87 REVISED: COMPLETED:
RECOMMENDATIONS TO REGIONAL OFFICES FOR CASE-BY-CASE APPLICATIONS
-ANNUAL REPORT (FY 87)
2058 DUE: 12/30/87 REVISED: COMPLETED:
RECOMMENDATIONS TO THE DIRECTOR, OFFICE OF DRINKING WATER FOR
NATIONWIDE APPLICATION - ANNUAL REPORT FY 87
1656 DUE: 12/30/86 REVISED: COMPLETED: 12/01/86
Recommendations to Regional offices for case-by-case
applications - Annual Report (FY 86)
1-657 DUE: 12/30/86 REVISED: COMPLETED: 12/01/86
Recommendations to the Director, Office of Drinking Water
FOR NATIONWIDE APPLICATION--ANNUAL REPORT (FY 86)
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Page 30 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE
TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
C104 DRINKING WATER
E DRINKING WATER TECHNOLOGY
73 METHODS VALIDATION- DRINKING WATER QUALITY ASSURANCE
PPA (S)
RC
PROJECT
PROJECT OFFICER
METHOD VALID. DW/QA
A EMSL CINN
12 METHODS VALIDATION - DRINKING WATER/QUALITY ASSURANCE
JOHN WINTER
PHONE: 513-684-7325
******************************************
PLANNED START: 09/01/82 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Provide Quality Assurance Support to the Drinking Water Program.
RATIONALE: Precision and accuracy statements are needed for all analytical
methodology used to monitor MCLs and RMCLs in drinking water. Such statistics
shall be used for laboratory quality control charts, acceptance limits for
performance evaluations, and confidence limits for quality control samples.
APPROACH: A prime contractor is selected to obtain 10-15 participating labor-
atories. The prime contractor prepares, ampul concentrates which are spiked
into reagent and drinking water and subsequently analyzed by the participating
laboratories using the specified USEPA drinking water methodology. Youden's
non-replicate design of three sample pairs spread over the linear response
range is used in the method study. Data are processed using EMSL-Cincinnati's
specially designed computer programs (IMVS).
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati conducts method validation studies (MVSs) inhouse and
extramurally for drinking water (DW) analytes. The prime contractor, Bionetics,
Inc., was assigned the task order for preparation of spiking solutions, water
samples, instructional materials, verification analyses, formal conduct of the
study, and screening of the study data from ten participating laboratories.
EMSL-Cincinnati obtains the ten participating laboratories through the
competitive procurement process based on technical qualifications, performance
evaluation, and costs. EMSL-Cincinnati evaluates the results, conducts
computerized data treatment, and prepares the final report.
ACCOMPLISHMENTS AND RESULTS TO DATE:
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Page 31 of 65
Status Report - MVSs (Chemical and Microbiological) (Ray Wesselman): At the
request of the Office of Drinking Water (ODW), preliminary collaborative studies
were conducted to determine the interlaboratory method detection limits (IMDLs)
for analytes in Method 504 (1,2-Dibromoethane (EDB) and Dibromochloropropane
(DBCP)), Method 505 (Pesticides), Method 515 (Herbicides) and Method 531
(Aldicarbs). The IMDLs were provided to ODW for use in drafting Phase 2,
synthetic organic chemicals (SOC) regulations.
Formal Interlaboratory MVSs are being conducted on DW Methods: 502.2 (Volatile
Halogenated and Aromatics using the photoionization detector and the Hall
detector in series) 504 (EDB and DBCP) Organics - gas chromatograph/electron
capture (GC/EC) microextraction), and 505 (Organohalide Pesticides with
microextraction), 515 (Herbicides - GC derivatization), 524.2 (Volatiles by
GC/mass spectrometer (MS) and 531 (N-methyl carbamayloxines and N-methyl
carbamates by post column derivatization high performance liquid chromatography
(HPLC)). Award of contract was made in June, 1987 to ten laboratories.
STATUS OF LABORATORY OUTPUTS: None
STATUS AND SCHEDULE OF DELIVERABLES:
1659 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
STATUS REPORT; INTERLAB METHOD VALIDATION STUDIES (CHEMICAL AND
MICROBIOLOGICAL) FOR DRINKING WATER METHODS
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Page 32 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE
TITLE
BUDGET SUB-ACTIVITY
C104
DRINKING WATER
ISSUE
E
DRINKING WATER TECHNOLOGY
PPA (L)
74
INTERLAB0RAT0RY PERFORMANCE EVAL. - DRINKING WATER QA
PPA (S)
INTERLAB. PE DW/QA
RC
A
EMSL CINN
PROJECT
13
INTERLABORATORY PERFORMANCE EVALUATION - DRINKING WATER QA
PROJECT OFFICER
PAUL
BRITTON PHONE: 513-684-7325
**********
* * * *
****************************
PLANNED START: 09/01/82 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Monitor the ability of the USEPA regional, primary state and intrastate
laboratories to perform chemical and microbiological analyses for contaminants
regulated under the NIPDWR.
RATIONALE: The regions and states can only effectively operate the
Certification Program under the SDWA if they themselves have the knowledge and
ability to perforin all of the analyses required for certification.
APPROACH: Design, prepare and verify by analyses samples for performance
evaluation studies, analyses of trace metals, nitrate/fluoride, chlorinated
pesticides, herbicides, turbidity, residual chlorine, sodium and corrosivity.
Conduct annual interlaboratory performance evaluation studies for principal
state (50 states) and USEPA regions required to conduct chemical and
microbiological analyses of public drinking water supplies. Evaluate regional
laboratories for capabilities to provide quality data in support of the NIPDWR
monitoring program. Compare the analytical results from participating
laboratories against acceptance limits generated from previous PE studies.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS:
EMSL-Cincinnati is responsible for providing support for the Drinking Water
Certification Program. This support consists of preparing and verifying
performance evaluation (PE) samples and conducting PE studies with 1100+ water
supply (WS) laboratories across the United States twice annually (WS and WS
Microbiology studies). The returned data are evaluated and individual
laboratory reports of performance computed and returned to interested parties.
The studies include all maximum contaminant level (MCL), monitoring and control
analytes. Bionetics, Inc., was selected as the contractor to supply
microbiological PE samples for full-volume and/or lyopholized cultures on
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Page - 33 of 65
July 1, 1985.
Computer programming and data processing support are obtained through the
Agency's ADP contract with Computer Sciences Corporation. All project
activities are on or ahead of schedule.
ACCOMPLISHMENTS AND RESULTS TO DATE:
Microbiological PE Study (Gary Collins): On April 13, 1987, as scheduled,
samples for WSM10 were mailed to 155 USEPA and state laboratories. Results were
obtained from 137 laboratories by the deadline, an 88 percent response rate.
Reports will be provided to the USEPA regional Quality Assurance
Coordinators/Officers by July 27, 1987. All activities are on schedule.
Conduct PE Studies for Drinking Water Laboratories (Paul Britton): Samples for
WS020 and WSM10 were shipped to -1-248 laboratories on April 6, 1987, as
scheduled. For the first time, samples were available for certification for
volatile organic chemicals (VOCs). Data were returned by 1041 laboratories
before the deadline on June 8 for a good 83.4 percent response. Three
copies of the individual laboratory PE reports will be returned by July 27 to
the involved regional Quality Assurance Coordinators and contract project
officers for their distribution and use to support certification decisions.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2189 DUE: 09/30/87 REVISED: COMPLETED: 08/14/87
PERFORMANCE EVALUATION STUDY REPORT FOR WATER SUPPLY STUDIES WSM09,
WSM 10, WS019 .AND WS020 FOR FY 87
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Page 34 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
'k'k'k'k'k'k'k'k'k'kitlcic'kicic'k'k'k'k'kitic'k'k'k'k'k'k'k'k'k'k'k'kic'k'k'k'k'k'k
CODE
TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
C104 DRINKING WATER
E DRINKING WATER TECHNOLOGY
75 LABORATORY CERTIF. PROGRAM FOR DRINKING WATER QA
PPA (S)
RC
PROJECT
PROJECT OFFICER
LAB. CERT. DW/QA
A EMSL CINN
14 LABORATORY CERTIFICATION PROGRAM FOR DRINKING WATER
J. J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 09/01/83 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Certification of laboratories to do drinking water analyses. Section
141.28 of the NIPDWR states that for compliance purposes, samples may be con-
sidered only if they have been analyzed by an approved laboratory.
RATIONALE: The "Manual of Interim Certification of Laboratories Involved in
Analyzing Public Drinking Water Supplies" EPA 600/8-78-008 states that the
Environmental Monitoring and Support Laboratory - Cincinnati is responsible
for e'valutation of the Regional Laboratories by conducting oh-site visits at
each laboratory once annually and conducting performance evaluation studies
annually.
APRROACH: Evaluate regional laboratories and personnel for capabilities to
provide quality data in support of the NIPDWR's monitoring program. Approve
regional laboratories to perform analyses on the regulated contaminants covered
under NIPDWR. Certify regional staff. Review regional certification program
and laboratory performance records. Evaluate ten regions annually.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
The Environmental Monitoring and Support Laboratory - Cincinnati
(EMSL-Cincinnati) evaluates and certifies regional laboratories and personnel
for capability to provide analytical data in support of drinking water
regulations. Extramural activity for this project involves preparation of
performance evaluation samples and support for the microbiology laboratory
certification training course. The majority of activity under this project is
in-house. The effort includes: development and presentation of drinking water
certification training courses, including course manual, for chemistry and
microbiology; on-site visits to the regional laboratories for the purpose of
certifying them to do the analyses required under the National Interim Primary
Drinking Water Regulations (NIPDWR); and quality assurance support.
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Page 35 of 65
LABORATORY CERTIFICATION TRAINING COURSES--The chemistry course was held twice
during the weeks of June 15 and 22. One session was held for microbiology
during the week of June 8. These provided regional, state, and some other
agency chemists and biologists with the opportunity of becoming certified to
audit state and local laboratories for drinking water analyses.
REGIONAL LABORATORY ON-SITE VISITS--(Items #2062[A] and 2063[A]). Plans are
being made to begin the next round of regional on-site evaluations during the
second half of FY 87. EMSL-Cincinnati staff are continuing to work with the
Office of Drinking Water (ODW) in revising the Drinking Water Certification
Manual. A workshop was conducted on April 1 and 2. Approximately 110 state,
local, U. S. Environmental Protection Agency (USEPA), and other government
laboratory representatives attended. Several EMSL-Cincinnati staff members
assisted in presenting the workshop, preparing reports, and again revising the
certification based on input during the workshop.
STATUS OF LABORATORY OUTPUTS; None.
STATUS AND SCHEDULE OF DELIVERABLES:
2062 DUE: 12/30/87 REVISED: COMPLETED:
CONDUCT LABORATORY CERTIFICATION FOR 10 REGIONS - SUMMARY REPORT
FY 87
2063 DUE: 12/30/87 REVISED: COMPLETED:
LABORATORY CERTIFICATION FOR FY 1987 -- ANNUAL REPORT
1663 DUE: 12/30/86 REVISED: COMPLETED: 12/22/86
Conduct Laboratory Certification for 10 Regions - Summary
Report (FY 86)
1664 DUE: 12/30/86 REVISED: COMPLETED: 12/22/86
Laboratory Certification for FY 1986 - Annual Report
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Page 36 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
* * ** **************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
C104 DRINKING WATER
E DRINKING WATER TECHNOLOGY
70 RADIOCHEMICAL PROC. - DRINKING WATER - QUAL. ASSURANCE
PPA (S)
RC
PROJECT
PROJECT OFFICER
RAD. CHEM. DW/QA
A EMSL CINN
15 RADIOCHEMICAL PROCEDURES - DRINKING WATER-QUALITY ASSURANCE
J. J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 09/01/80 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: The ODW is conducting an occurrence survey for radionuclides and
inorganic chemicals. This survey is called the National Inorganic Radio-
nuclide Survey (NIRS).
RATIONALE: Analyses will be conducted for the six radiochemical analytes and
33 inorganic elements.
APPROACH: These analyses will be conducted at a rate of 600 samples per year.
Referees will analyze radionuclides in drinking water.
The analyses will be conducted under highly controlled quality assurance
conditions.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
The Environmental Monitoring and Support Laboratory - Cincinnati (EMSL-
Cincinnati) is participating in the National Inorganics and Radionuclide Survey.
Drinking water samples are delivered by the Technical Support Division (TSD) to
EMSL-Cincinnati for analysis of 33 inorganic and 6 radionuclide analytes. All
efforts under the project are being carried out in-house. Activities include:
working closely with TSD in project planning and selection of analytical
methods for the survey, performing appropriate sample analyses; reviewing and
analysis of data; and report writing. The main part of the survey has been
completed. Special samples for Ra 228 are now being analyzed.
NATIONAL INORGANICS AND RADIONUCLIDES SURVEY--EMSL-Cincinnati continues to
provide analytical technical assistance to other U. S. Environmental Protection
Agency (USEPA) laboratories and state and local laboratories. During the
quarter, 6 samples were analyzed for gross alpha and beta, 64 for uranium, 6
for Ra 226, and 95 for radon.
The analyses of samples for Ra-228 analysis has started. The data from this
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Page 37 of 65
study are to be used for the Ra-228 Occurrence Model. The status of these
analyses is noted in the following table.
Quality
Samples Samples Samples Completed- Control Samples
Analysis Received Started Completed This Quarter This Quarter
Ra-228 255 255 224 102 14
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2051 DUE: 09/30/87 REVISED: COMPLETED: 09/14/87
Radio-chemical analytical data - Report of occurrance study
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Page 38 of 65
CLIENT OFFICE 054
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
*¦ *****************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
******************************************
PLANNED START: 10/01/84 PLANNED END: 01/01/00
******************************************
CI04 - DRINKING WATER
E DRINKING WATER TECHNOLOGY
71 COLIFORM ANALYSIS-DRINKING WATER-QUALITY ASSURANCE
COLIFORM ANAL. DW/QA
A EMSL CINN
16 COLIFORM ANALYSIS - DRINKING WATER - QUALITY ASSURANCE
CORNELIUS I. WEBER PHONE: 513-684-7337
PROJECT DESCRIPTION:
GOAL: Provide improved methods for the microbiological analysis of
drinking water, correct method deficiencies, evaluate sample holding
conditions, and develop quality assurance procedures.
RATIONALE: This project addresses Agency needs for scientifically valid
and legally defensible microbiological data on water quality in support of
the Safe Drinking Water Act.
APPROACH: Develop or modify, evaluate, select, and standardize methods for
the detection and enumeration of indicator organisms and pathogens in
drinking water. Alternate methods for total coliforms will be evaluated
against standard methods. Appropriate methodologies-will be recommended for
use in revised drinking water regulations. As a result of inhouse and
extramural research, recommendations will be made-on holding limitations
for drinking water samples to assure reliable analytical results. Rapid
methods, such as gene probes and monoclonal and polyclonal antibodies, will be
developed for the detection and verification of pollution indicators and
waterborne pathogens.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
There are no FY-87 extramural funds for this project. However, two tasks from
previous years are still active: one with McNeese State University to determine
sample holding effects on microbial populations, and one with the University of
Rhode Island, to develop an improved membrane filter (MF) medium for total
coliforms (TCs). The draft final reports from both projects were received and
are being peer reviewed.
The report, "Comparison of the Hydrophobic-Grid Membrane Filter Procedure and
Standard Methods for Coliform Analysis of Water", was published in the May, 1987
issue of the Journal of Applied and Environmental Microbiology. The study
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Page 39 of 65
found that total and fecal coliform recoveries by the hydrophobic-grid membrane
filter (HGMF) and standard MF methods did not differ significantly over all
sample types, and the HGMF method offers no advantage for the analysis of
drinking water.
A report on the results of a preliminary multilaboratory evaluation of new
alternative media for the TC MF test was completed. The results indicated that
the proposed alternative media and the standard M-Endo medium provided similar
recoveries. Improved formulations and a lyophilized natural sample will be used
in a formal collaborative testing procedure (2054A).
The Section staff directed a course, "Drinking Water Laboratory Certification
for Microbiology", June 8-15, 1987, attended by twenty regional and state
laboratory certification officers. Several Agency microbiologists participated
in lectures and panel discussions.
The Microbiology Section sponsored a seminar at the Andrew W. Breidenbach
Environmental Research Center, May 26, 1987, on "The Importance of Membrane
Filter Manufacturing and Characterization of their Use" by Ricardo Alfonso,
Sartorius Filters, Inc.
STATUS OF LABORATORY OUTPUTS
The extramural report on an improved MF medium for coliforms in drinking water
from the University of Rhode Island is being peer reviewed.
A revision of the final report on the research project, "The Effects of Storage
on the Bacteriologic Quality of Potable Water Samples", from MCNeese State
University has been peer reviewed and is being revised again.
B812 DUE: 03/87 "Improved Membrane Filter Medium for Coliforms in Drinking
Water". Report distributed for peer review.
STATUS AND SCHEDULE OF DELIVERABLES:
2054 DUE: 06/30/87 REVISED: COMPLETED: 06/30/87
REPORT ON MULTI-LAB0RT0RY EVALUATION OF NEW ALTERNATIVE MEDIA
FOR TOTAL COLIFORM MF TEST
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Page 40 of _6j5
CLIENT OFFICE
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE
TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
PLANNED START: 09/01/84 PLANNED END: 09/01/88
******************************************
D109
Q
01
A
17
JOHN WINTER
HAZARDOUS WASTE
QUALITY ASSURANCE
QA TO SUPPORT WASTE CHARACTERIZATION
QUALITY ASSURANCE
EMSL CINN
QA TO SUPPORT WASTE CHARACTERIZATION IN SW 846
PHONE: 513-684-7325
PROJECT DESCRIPTION:
GOAL: Section 3001 of the RCRA requires the Agency to determine the
chemical composition of wastes, detect trace levels of toxic constituents
and to be able to rapidly screen for hazardous characteristics of spilled
materials. The lack of standardized and validated methods emphasizes the
immediate need for a comprehensive program to assure that data of known
quality are being collected.
RATIONALE: With the transfer of permitting and enforcement from USEPA to the
states, standardized methods and QA support are needed to assist the states
performing the required measurement and monitoring functions and in obtaining
legally defensible data.
APPROACH: Develop, evaluate and distribute analytical standards and
reference materials for Appendix VIII compounds. Perform validation
studies on analytical methods. Develop and distribute quality control
and performance evaluation samples for Appendix VIII compounds
Provide quality assurance support to Regions.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati produces and distributes quality control (QC) samples and
calibration standards and conducts performance evaluation (PE) studies to
support the Office of Solid Waste (OSW) monitoring program. A contract was
awarded to Bionetics, Inc., in July 1985, to produce and distribute QC samples
for analytes of interest to OSW and other programs of the Agency. Sample
concentrates and full-volume PE samples are produced and distributed to
evaluate contract laboratories performing analyses and for the Ground Water
Monitoring Survey being conducted by the Office of Solid Waste and Emergency
Response (OSWER). Approximately 58 sites will be sampled in FY86 and FY87.
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Page 41 of 65
Northrop Services, Inc., produces and distributes organic calibration standards
for the Resource Conservation and Recovery Act (RCRA) and other Agency programs.
Contracts-were awarded for four organic and four inorganic referee laboratories
to verify the true concentration of analytes in QC and PE samples and
calibration standards produced inhouse and by contractors. The National Bureau
of Standards (NBS) is in the fourth year of an interagency agreement (IAG) to
establish traceability for RCRA - Appendix VIII analytes in PE and QC samples.
ACCOMPLISHMENTS AND RESULTS TO DATE:
USEPA Repository for Toxic and Hazardous Materials (Harry Kolde): Eleven new
RCRA chemicals were ampuled and four others re-ampuled to replenish stocks.
The Repository distributed 20,400 standard solutions to 420 USEPA, USEPA
contract, state, and local laboratories. Sixteen RCRA chemicals were sent to
referee laboratories for purity assay.
QC Samples (Ray Wesselman): A total of 46,330 QC samples were sent to USEPA,
USEPA contractor, regional, state, and local laboratories. Approximately 24,000
sets of various polychlorinated biphenyls (PCBs), PCBs in oils, and pesticides
were produced and verified.
Conduct Referee Analyses (Harry Kolde): (1) spiking/calibration solutions for
0SW compounds, 199 ordered, none completed; (2) QC and PE samples, 861 ordered,
249 completed; and (3) surrogates for USEPA Methods 624 and 625, 9 ordered,
none completed.
Traceability to NBS (Ed Berg): A priority list of PE and QC samples requiring
NBS analyses was agreed upon. Analyses have begun on selenium in fish samples.
NBS will produce 3,000 sets of polychlorinated biphenyls (PCBs) congeners for
USEPA.
Preparation of PE Samples for RCRA Contract Laboratories (Harold Clements):
Solid Waste Interlaboratory Comparison Study X was initiated April, 1987.
Organic and inorganic samples were sent to 54 participating laboratories on
April 16, 1987. Reference values and statistical estimates were generated by the
QA Branch and its referee laboratories and supplied to Office of Solid Waste
(0SW) for use in final evaluation of results.
Ground Water Monitoring Task Force (GWMTF) (Ray Wesselman): No activity to
report this quarter.
SW Guidance Manual on Organization and Management of Laboratories QA Program
(John Winter): Target dates have been set. A text outline was presented to 0SW
and approved. Additional source materials were being reviewed for
appropriateness. A first draft is in preparation with deadline for August.
STATUS OF LABORATORY OUTPUTS:
Output 1688[B] Annual Report on Traceability to NBS due 12/86 was completed.
Output 1688[C] Annual Report on PE Studies for RCRA Contract Laboratories due
12/86 was completed. Output 1688[D] Annual Report on Referee Analytical
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Page 42 of 65
Services due 12/86 was completed. Output 1688[E] Status Report on PE Studies
for Ground Water Monitoring Program due 12/86 was completed.
STATUS AND SCHEDULE OF DELIVERABLES:
2152 DUE: 09/30/87 REVISED: COMPLETED: 09/28/87
GUIDANCE MANUAL ON ORGANIZATION AND MANAGEMENT OF LAB QA PROGRAM
1986 DUE: 12/31/86 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT ON CALIBRATION STANDARDS FROM THE USEPA REPOSITORY OF TOXIC
AND HAZARDOUS MATERIALS (APPENDIX VIII COMPOUNDS)
1987 DUE: 12/31/86 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT ON QUALITY CONTROL AND SAMPLE PROGRAM FOR RCRA (APPENDIX
VIII) COMPOUNDS
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CLIENT OFFICE
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY: D109
ISSUE: I
PPA (L): 12
PPA (S):
RC: A
PROJECT: 18
PROJECT OFFICER: ED BERG
******************************************
PLANNED START: 10/01/82 PLANNED END: 10/01/87
*******************************************
HAZARDOUS WASTE
WASTE IDENTIFICATION
VALIDATE METHODS FOR WASTE CHARACTERIZATION IN SW 846
EVAL/VALIDATE METHOS
EMSL CINN
VALIDATE METHODS FOR WASTE CHARACTERIZATION IN SW 846
PHONE: 513-684-7325
PROJECT DESCRIPTION:
GOAL: Evaluate/validate protocols and procedures for solid waste
characterization to assure legally defensible and scientifically
accurate data.
RATIONALE: Provide precision and accuracy statements for OSW analytical
methodology.
APPROACH: EMSL-Cincinnati will obtain 10-12 laboratories to participate
in a collaborative study, will prepare samples for spiking into selected
matrices, process analytical data, and will develop precision and accuracy
statements for metals in water and sludge and GC\MS Methods 8240 and 8270.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
This is an extramural project to conduct interlaboratory method validation
studies (IMVSs) for SW-846 analytical methodology. Request for proposals
(RFPs) are to be developed to conduct studies for SW-846 Methods 3005, 3006
(Trace Metals in Ground Water) 3010 (Trace Metals in Ambient Water), 3050
(Trace Metals in Sludges), 8270 (Base/Neutrals), and 8240 (Purgeables). The
studies use Bionetics, Inc., as a prime contractor to prepare samples and
instructions and conduct the study for ten participating laboratories selected
by EMSL-Cincinnati. The resulting data from the participants will be processed
by the Quality Assurance (QA) Branch, EMSL-Cincinnati, using the customized IMVS
computer programs which provide standard data treatments to reject outliers
and generate regression equations for precision and bias statements.
ACCOMPLISHMENTS AND RESULTS TO DATE:
IMVS for SW-846, Methods 8240/5030 Purge and Trap (100 Compounds) (Ray
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Page 44 of 65
Wesselman): Contracts were awarded to ten study participant laboratories in
late June, 1987. The analytes will include all Appendix IX compounds which are
analyzable with the method.
IMVS for SW-846, Methods 8270/3510 (59 Compounds) (Ray Wesselman): Data from
all ten laboratories was received in late June, 1987. Statistical review of
data to occur in July with rough draft of report in August.
IMVS for SW-846, Methods 3005, 3050, 3010; for Metals in Ambient and Ground
Water, and in Sludges (Ed Berg): Nine contractors were selected to participate
in the IMVSs for SW-846 Methods 3005,' 3010, and 3050. Participants have
completed analyses of samples by Method 3005 and returned data is being
processed by IMVS computer program. Bionetics, Inc., is currently producing the
solid samples for use in the study for Method 3050 which will commence next
quarter.
STATUS OF LABORATORY OUTPUTS:
Output 1696[B] Conduct Interlaboratory Method Validation Studies for SW 846 -
Methods 8240 and 8270 due 12/87, completed 12/86. Output 1695[B] Conduct
Interlaboratory Method Validation Studies for SW 846 - Metals Dissolution due
12/87, completed 12/86. Output 1360[B] Report on Status of Reference Materials
for Chlorinated and Brominated Dibenzo-p-Dioxins due 12/86, completed 12/86.
STATUS AND SCHEDULE OF DELIVERABLES:
2068 DUE: 12/30/87 REVISED: 09/30/87 COMPLETED: 09/17/87
STATUSREPORT-INTERLABORATORY METHOD VALIDATION STUDY FOR SW-846, METHOD
8240/5030 PURGE AND TRAP (100 COMPOUNDS)
2069 DUE: 12/30/87 REVISED: 09/30/87 COMPLETED: 09/17/87
STATUSREPORT - INTERLABORATORY METHOD VALIDATION STUDY FOR SW-846, METHOD
8270/3510 (59 COMPOUNDS)
2071 DUE: 09/30/87 REVISED: COMPLETED: 09/17/87
STATUS REPORT: INTERLABORATORY METHOD VALIDATION STUDY FOR SW-846 METHOD
3006, 3050, 3010; FOR METALS IN AMBIENT AND GROUND WATER, AND IN SLUDGES
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CLIENT OFFICE
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
PPA (S)
RC
PROJECT
PROJECT OFFICER
~ lit****************************************
PLANNED START: 10/01/82 PLANNED END: 12/01/99
D109
I
12
A
19
HAZARDOUS WASTE
WASTE IDENTIFICATION
VALIDATE METHODS FOR WASTE CHARACTERIZATION IN SW 846
EVAL/VALIDATE METHOS
EMSL CINN
VALIDATE METHODS FOR WASTE CHARACTERIZATION IN SW-846
J. J. LICHTENBERG
PHONE: 513-684-7306
PROJECT DESCRIPTION:
GOAL: -RCRA requires the Agency to determine the chemical composition of waste
and to detect trace levels of toxic constituents in groundwater and to be able
to rapidly screen for hazardous constituents.
RATIONALE: The.lack of standardized validated methods emphasizes the immedi-
ate need for a comprehensive program to assure that data of known quality are
being collected. Improved laboratory methods will be required to furnish the
quality assured data.
APPROACH: Evaluate and validate protocols and procedures to assure legally
defensible and scientifically accurate data for hazardous waste measurement.
This includes protocols for both organic and inorganic analyses. Protocols
in "Test Methods for Evaluating Solid Waste" Document (SW-846) will be
evaluated for precision, accuracy, and reliability on a wide variety of solid
waste matrices.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
The major portion of work under this project is conducted extramurally. Two
contractors, Battelle-Columbus and S-Cubed, are responsible for performing
evaluation and single laboratory validation (development of accuracy and
precision data) of the SW-846 solid waste methods. The contracts are flexible
task order type contracts covering inorganic and organic chemical and physical
methods.
GAS CHROMATOGRAPH (GC)/MASS SPECTROMETER (MS) SUITABILITY STUDIES FOR METHODS
8240 AND 8270--Previous reports have identified 39 additional compounds that
could be chromatographed with 31 priority pollutants using the conditions
specified in 8240 and 148 compounds that could be added to the priority
pollutants that chromatograph using the conditions of 8270 (Item # 2153[A]).
The final report on this work is planned for delivery 9/87.
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PURGEABLES BY METHODS 8010, 8015, AND 8020--In response to a request by the
Office of Solid Waste, Battelle prepared a special report for inclusion into
the public record for the Appendix IX final regulation. The revised final
report is expected early in the fourth quarter. A request will be made to
modify the delivery date.
CAPILLARY COLUMN UPDATE OF METHOD 8240--Experimental work has been completed
but the draft final report has been delayed by the need to prepare pesentations
for the Solid Waste Symposium. The draft report is expected in August.
SINGLE-LABORATORY VALIDATION OF METHOD 8240/5030--A task to generate single
laboratory validation data for Method 8240 with a wide-bore capillary column has
been initiated. Preliminary retention time and response factor data have been
gathered and matrix validation has begun. The project is expected to stay on
schedule.
EVALUATION OF SW-846 METHODS FOR VOLATILES IN SOLID MATRICES--Due to emergency
personal leave taken by the principal investigator, start of the project is
delayed until October.
EVALUATION OF METHOD 3510/3520 WITH GC--Those Appendix VIII/Michigan List
compounds that could be chromatographed using the Superfund conditions for
Method 8270 were screened for extractability and aqueous stability to provide
information to support Appendix IX. The revised final report was received in
early June. The project summary is due in July.
EVALUATION OF ALTERNATE EXTRACTION pH LEVELS--A comparative study of the four
extraction procedures (pH variations) is being conducted on the Appendix IX
list of compounds. The extraction procedures being examined are: (1)
Acid/Neutral + Bases (A/N+B) - Initial extraction at pH 2 followed by
extraction at pH 11; (2) Base/Neutral + Acids (B/N+A) - Initial extraction at
pH 11 followed by extraction at pH 2; (3) Neutral + Acids (N/A)- Initial
extraction at pH 7 followed by extraction at pH 2; (4) Extraction at pH 4 only.
The results will be statistically evaluated. A status report has been
written on existing data for presentation at the Solid Waste Symposium in
Washington, D.C. in July. Data to complete the report will be collected in the
fourth quarter.
EVALUATION OF GEL PERMEATION CHROMATOGRAPHY (GPC) CLEANUP-Elution profiles of
the Appendix VIII compounds were completed. Acetone showed a dramatic influence
on the gel as it causes shrinkage. Other ketones and similar laboratory
solvents will be tested for column effects in the next quarter.
EVALUATION OF SW-846 EXTRACTION METHOD FOR SEMIVOLATILES IN SOLIDS--(Item
#2155{A]) The Appendix VIII and Michigan List analytes will be spiked into
solid Resource Conservation and Recovery Act (RCRA) wastes and extracted to
determine the accuracy and precision of the method(s) in SW-846. The original
plans to conduct this activity in parallel with the validation of 8270 in water
have been modified due to the delays in establishing the final pH for
extraction of water and the need to complete the 8270 validation in a timely
manner. It will now be a follow-on task at S-Cubed to commence in October, when
the 8270 study will be finished.
EVALUATION OF GENERIC HEATED PURGE METHOD--(Item #2154[A]) METHODS 8320 AND
8330--Single laboratory testing of the GC/MS method for selected Appendix VIII
analytes has been completed and will be incorporated into a report at the
Solid Waste Symposium. The draft final report is to be submitted in August.
FORMALDEHYDE AND OTHER ALDEHYDES--The task to complete referee validation of
the method was discontinued until final experiments testing effects of filtra-
ion on waste samples using adsorption cartridges are completed. Experiments in
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Page 47 of _65^
comparing extractions using MTBE and methylene chloride for monitoring
background levels using carbon filtered tap water and commercially available
water matrices have been completed. Spiked real-world samples were also
analyzed.
STATUS OF LABORATORY OUTPUTS: 1695[C] GC/MS Suitability Testing of RCRA
Appendix VIII and Michigan List Analytes--DUE: 4/87 STATUS: To be delivered July
1987. 1695[D] Validation of SW-846 Methods 8010, 8015, and 8020--DUE: 5/87
STATUS: To be delivered 9/87. 1695[E] Screening of Semivolatile Organic
Compounds for Extractability by EPA-Method 3510 and Aqueous Stabi1i ty--DUE:
5/87 STATUS: To be delivered 9/87.
STATUS AND SCHEDULE OF DELIVERABLES:
2153 DUE: 09/30/87 REVISED: 05/30/88 COMPLETED:
FINAL REPORT ON SINGLE LAB VALIDATION OF METHODS 8240 AND 8270 FOR APPENDIX
IX COMPOUNDS
2154 DUE: 12/30/87 REVISED: COMPLETED:
FINAL REPORT ON SINGLE LAB VALIDATION OF METHOD 8240 AND HEATED PURGE
AND TRAP METHODS FOR APPENDIX VIII PLUS THE MICHIGAN COMPOUNDS LIST
2155 DUE: 09/30/88 REVISED: COMPLETED:
FINAL REPORT ON SINGLE LAB VALIDATION OF METHOD 8270 FOR APPENDIX VIII
PLUS THE MICHIGAN COMPOUND LIST
1695
DUE: 12/30/86 REVISED: COMPLETED: 12/22/86
PROGRESS REPORT - GENERIC GC/MS METHODS FOR VOLATILE AND SEMI-
VOLATILE ORGANIC COMPOUNDS
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CLIENT OFFICE
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
D109 HAZARDOUS WASTE
I WASTE IDENTIFICATION
14 DEVELOP NEW METHODS FOR SW 846
PPA (S)
RC
PROJECT
PROJECT OFFICER
DEVELOP METHODS
A EMSL CINN
20 DEVELOP NEW METHOD FOR SW-846
J. J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 10/01/82 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Develop methodologies and protocols for analyzing and monitoring
hazardous wastes including Appendix VIII compounds incorporating advanced
techniques for more rapid and accurate methods. Develop and evaluate extract-
ive procedures for organic wastes. Develop GC(LC)MS and GC fourier transform
infrared/MS for cost effective analysis of groundwater and wastes.
RATIONALE: Monitoring methods require developing, adopting and assessing
technologies applicable to monitoring groundwater and complex wastes contain-
ing a significant portion of nonvolatile components. A broad spectrum of
volatile, semivolatile compounds and landfill leachates containing intact,
biodegraded or chemically altered components. Various wastes experience
storage, treatment and disposal differences which require rapid, cost effective
and/or unique monitoring methods.
APPROACH: Evaluate techniques and procedures for extraction, isolation, and
measurement for rapid screening of organic chemicals in solid wastes. Compare
existing methods for cost effectiveness.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
Cornell University, under a cooperative agreement, has a project titled "The
Determination of Intractable Organic Compounds by Direct Liquid Injection
(DLI)/Thermospray Liquid Chromatography (LC)/Mass Spectrometry (MS) and
LC/MS/MS". In-house efforts consist of research to develop analytical
procedures to identify and to measure nonvolatile and non-gas chromatographable
hazardous compounds in environmental samples.
The monoclonal antibody immunoassay for chlorinated dibenzodioxins (CDDs) and
chlorinated dibenzofurans (CDFs) was extensively tested using spiked California
soils and contaminated Missouri soils provided by Region 7. During this
testing unacceptably variable results were obtained, and it was necessary to
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Page 49 of 65
find the source of the problem before field testing could begin. The sample
extraction and preparation procedure was reviewed and modified to eliminate
sources of variability and to allow automation of the procedure to further
reduce variability. The testing resumed late in the quarter and will continue
until consistent assays with good precision can be obtained.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2074 DUE: 12/30/87 REVISED: COMPLETED:
ORGANIC CHEMICAL SCREENING METHOD EVALUATION
1676 DUE: 12/30/86 REVISED: COMPLETED: 12/22/86
REPORT ON DEVELOPMENT OF ANALYTICAL METHODS FOR DIOXINS AND
FURANS
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Page . 50 of 65
CLIENT OFFICE 063
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
•k'kicieielcieieieicicieicicie'k'kieieic'k'kieic'k'k'k'k'k'kit'k'k'k'kic^k'k'ieieieie
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
Y105 HAZARDOUS SUBSTANCES
A PROVIDE TECHNIQUES&PROCEDURES FOR SITE&SITUATION ASSESSMENT
04 TECHNIQUE FOR SITE ASSESSMENT
PPA (S)
RC
PROJECT
PROJECT OFFICER
SITE ASSESSMENT
A EMSL CINN
21 Monitoring for Site Assessment
J. Lichtenberg
PHONE: 008-684-7306
******************************************
PLANNED START: 06/01/81 PLANNED END: 12/30/99
******************************************
PROJECT DESCRIPTION:
GOAL: The success of removal and remedial actions depends on an accurate
definition of the kinds and severity of the problem. The latest protocols,
techniques, instrumentation, for sampling and analysis and remote monitoring
must be applied to provide the decision maker with scientifically accurate
information.
RATIONALE: Methods modifications and evaluations of methods are needed to
increase the efficiency of site.and situation assessment and cost-effective-
ness. Systems are required to enhance the quality of data acquisition and the
speed and reliability of data analysis. Water monitoring represents over 50%
of the analytical work at most uncontrolled hazardous waste sites.
APPROACH: Protocols, techniques and methods will be prepared, tested, modi-
fied, and documented. Emphasis will be on automated methods to ensure cost
effectiveness, speed, consistency, and reliability. Water and water related
media will be given high priority along with CERCLA analytes published in the
Federal Register.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
Work began on a multilaboratory test of a gas chromatography (GC)/mass
spectrometry (MS) method for semi volatiles, pesticides, and polychlorinated
biphenyls (PCBs) in a single extraction and analysis. Included in the method is
a modified pH adjustment sequence and a gel permeation chromatography cleanup
step with detailed directions and required quality control. Included in the
multilaboratory test is the use of software to automate the interpretation of
some of the data, software to automate all the computations, and software to
place all the results in the proposed Agency standard format for delivery of
monitoring data on computer readable media. The participating laboratories,
which include seven Superfund contract laboratories, Region 7, and Battelle
Columbus, received spiked water and sediments, sediments environmentally
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Page 51 of 65
contaminated, all calibration solutions, and all software. The results will be
evaluated during the last quarter.
STUDY TO OPTIMIZE EXTRACTION PROCEDURES--Preliminary results of these tests
revealed recoveries that were lower than expected. Problems in the extraction
procedures were identified and the affected tests are being repeated. This
study in reagent water is scheduled for completion next quarter.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
1623 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
MONITORING METHODS FOR CERCLA SITE ASSESSMENT -- FY 86
2200 DUE: 11/30/87 REVISED: COMPLETED:
SUMMARY REPORT - MONITORING METHODS FOR CERCLA SITE ASSESSMENT
0501 DUE: 09/30/87 REVISED: COMPLETED: 09/28/87
PRESENTATION ON RESULTS OF STUDY TO OPTIMIZE EXTRACTION PROCEDURES FOR
CERCLA ANALYTES USING THE CLP GC/MS METHOD
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Page 52 of _65
CLIENT OFFICE 063
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY: Y105
ISSUE:
PPA (L):
F
06
HAZARDOUS SUBSTANCES
PROVIDE TECHNICAL SUPPORT TO ENFORCEMENT, PROGRAM & REGIONS
MONIT. FOR ENFORCE. & TECH. SUPPORT
PPA (S): TECH SUPPORT
RC: A EMSL CINN
PROJECT: 22 Monitoring for Enforcement and Other Technical Support
PROJECT OFFICER: J. Lichtenberg PHONE: 008-684-7306
******************************************
PLANNED START: 06/01/81 PLANNED END: 12/30/99
******************************************
PROJECT DESCRIPTION:
GOAL: All projects under CERCLA have potential enforcement requirements.
RATIONALE: The ORD is frequently required to provide scientific data for case
preparation.
APPROACH: The ORD will continue to provide oversight on settlement agreements
for example, Hyde Park, Love Canal. Services covering sampling, analytical
techniques, quality assurance samples, and review of monitoring plans will be
provided.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
Inhouse: The Quality Assurance (QA) Branch of EMSL-Cincinnati provides
performance evaluation (PE) samples to the officials in charge of the Hyde
Park-USEPA agreement, periodically, upon request. Hooker Chemical Company is
required to analyze for 21 target compounds in the PE samples to prove
proficiency in their analytical techniques used in managing the dump site. The
manager in Region 2 and the New York Department of Health officials. Inhouse
and extramural activities are conducted to cover sampling, analytical service
needs, and review of monitoring" plans.
ACCOMPLISHMENTS AND RESULTS TO DATE:
Sampling and Analytical Techniques (Bill Budde): Specifications were provided,
in response to a request from the Office of Emergency and Remedial Response
(OEER), for a format for inorganic data deliverables on computer readable
media. These specifications are compatible with the proposed Agency standard
format for delivery of monitoring data on computer readable media. In response
to another request, a file containing 44,205 Chemical Abstracts Service
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Page 53 of 65
Registry Numbers and corresponding chemical nomenclature was made available on
the National Computer Center mainframe at Research Triangle Park. This file is
needed by the contract laboratory program to verify information provided by
contract laboratories. A revised reference data base of 44,205 electron
ionization mass spectra was prepared and distributed. This collection contains
1944 new spectra, and 1700 old spectra were replaced with higher quality
spectra. Participated on a peer review panel to evaluate 28 proposals received
in response to an U.S. Environmental Protection Agency (USEPA) request for
applications for grants to develop measurement and monitoring methods for
Superfund sites. This Superfund research effort by non-profit research
organizations and education institutions was authorized under the Superfund
Amendments and Reauthorization Act of 1986. The peer review panel evaluated
the proposals according to their technical merit, and the probability that the
research could produce results that would support USEPA's hazardous waste site
clean-up program.
Evaluation of Performance/Hyde Park Litigation (Harold Clements): Samples were
analyzed by Occidental Chemical Company and data were returned to the QA Branch
for evaluation last quarter. All data were acceptable. No new activity on this
project this quarter.
STATUS OF LABORATORY OUTPUTS:
Output 1632[B] Annual Report of PE on Hyde Park Remedial Project of Contractor/
Enforcement Performance, due 12/86 was completed.
STATUS AND SCHEDULE OF DELIVERABLES:
1632 DUE: 11/30/86 REVISED: COMPLETED: 11/26/86
INTERNAL REPORT Water Monitoring Support Programs
2199 DUE: 11/30/87 REVISED: COMPLETED:
SUMMARY REPORT - ANALYTICAL TECHNICAL ASSISTANCE TO WATER MONITORING
SUPPORT PROGRAMS
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CLIENT OFFICE 063
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
Y105 HAZARDOUS SUBSTANCES
Q PROVIDE QUALITY ASSURANCE - SUPERVISE PROGRAM REQUIREMENTS
01 QUALITY ASSURANCE SUPPORT FOR CERCLA
PPA (S)
RC
PROJECT
PROJECT OFFICER
QUALITY ASSURANCE
A EMSL CINN
23 Quality Assurance Support for CERCLA
Ed Berg PHONE: 008-684-7325
******************************************
PLANNED START: 06/01/81 PLANNED END: 12/30/99
******************************************
PROJECT DESCRIPTION:
GOAL: The success of removal and remedial actions depends on an accurate
definition of the kinds and severity of the problem. The latest protocols,
techniques, instrumentation, quality assurance for sampling and analysis and
remote monitoring must be applied to provide the decision maker with
scientifically accurate information. A quality assurance program is needed to
provide support to the National Contract Laboratory Program which is
responsible for all contract chemical analyses under Superfund.
RATIONALE: Quality control samples and reference calibration standards are.
provided to Superfund contractors as independent checks on the analyst's
technique and application of the analytical procedure. Additionally
performance of Superfund contractors evaluated quarterly to assure the legal
defensibility of their monitoring data.
APPROACH: Quality control and performance evaluation samples are prepared and
distributed to Superfund contractors by Bionetics, Inc. Reference calibration
standards are prepared and distributed by Northrop Services, Inc. Quality
control samples and reference calibration standards are verified by referee
laboratories.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati provides quality assurance (QA) support to EMSL-Las Vegas which
is the lead laboratory responsible for QA support to the Superfund program.
EMSL-Cincinnati's QA program portion follows.
Quarterly performance evaluation (PE) studies are conducted on the Contract
Laboratory Program (CLP) laboratories which are providing analytical support
for Superfund site assessments. Bionetics, Inc., was awarded a new contract on
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Page 55 of 65
July 1, 1985, and assigned this task. A unit was set up to provide full-volume
PE samples containing about 20 volatiles, semi-volatiles, and pesticides.
Samples are prepared, packed in coolers, iced, and shipped to regional
personnel who label the samples and then mail them to the CLP laboratories as
double-blind samples. Bionetics, Inc., analyzes each sample set and reports
the true concentration and the analyzed concentration of each analyte to EMSL-
Las Vegas. CLP laboratory data are returned to EMSL-Las Vegas for evaluation.
Comprehensive Environmental Response Compensation and Liability Act (CERCLA)
compounds. Northrop Services operates the Repository for Toxic and Hazardous
Materials for EMSL-Cincinnati, which provides calibration standards for the
same list. Referee laboratories verify the true concentrations of analytes in
the QC samples and calibration standards.
Special series of sample concentrates and full-volume PE samples are produced
and distributed by Bionetics, Inc., to evaluate contract laboratories
performing analyses on samples from the Ground Water Monitoring Survey of
hazardous waste sites being conducted by the Office of Solid Waste and
Emergency Response (OSWER). Approximately 58 sites will be sampled in FY86 and
FY87.
ACCOMPLISHMENTS AND RESULTS TO DATE:
Evaluation of Superfund Contractors (Ed Berg): Approximately 70-80 sets of PE
samples are currently being prepared for the fourth quarter evaluation of CLP
laboratories.
USEPA Repository for Toxic and Harardous Materials (Harry Kolde): Eleven new
CERCLA chemicals were prepared as analytical standards and two were re-ampuled
to replenish low stocks. The Repository distributed 20,400 ampuls to 420
USEPA regional, state, local, and USEPA contract laboratories this quarter.
Ten sets of mixed polychlorinated biphenyl (PCB) congener standards were
supplied to support the New Bedford Harbor Study. Ten CERCLA chemicals were
sent to referee laboratories for purity verification.
QC Samples (Ray Wesselman): A total of 46,330 samples were distributed to
USEPA, USEPA contract, state, and local laboratories. Due to depletion of
inventories, 6200 ampuls of chlorinated hydrocarbon pesticides and 5900
PCBs Aroclor 1260 were produced and are being verified.
Conduct Referee Analyses (Harry Kolde): Third quarter analyses were as
follows: (1) spiking/calibration solutions for Superfund compouds, 187
ordered, none completed; (2) QC and PE samples, 813 ordered, 249 completed; and
(3) surrogates for USEPA GC/MS Methods, 9 ordered, none completed. Future
work - continue verification analyses.
Ground Water Monitoring PE Samples (Ed Berg): No activity for this quarter.
STATUS OF LABORATORY OUTPUTS:
Output 1626[B] Annual Summary Report - QA to Support CLP - PE Studies due 2/87
was completed. Output 1628[B] Annual Report on Referee Analytical Services due
12/86 was completed. Output 1626[C] Status Report - PE Study to Ground Water
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Page 56 of 65
Monitoring Program due 2/87 was coirmleted. Output 1628[C] Annual Report -
Repository for CERCLA Hazardous and Toxic Compounds due 12/86 was completed.
STATUS AND SCHEDULE OF DELIVERABLES:
1628 DUE: 12/31/86 REVISED: COMPLETED: 12/19/86
Annual Report - Quality Assurance to Support CERCLA
0509 DUE: 12/30/87 REVISED: COMPLETED:
ANNUAL REPORT - QUALITY CONTROL SAMPLE PROGRAM FOR CERCLA (FY 87)
0510 DUE: 12/30/87 REVISED: COMPLETED:
ANNUAL REPORT -- QUALITY ASSURANCE TO SUPPORT CONTRACT LAB PROGRAM (FY 87)
0511
DUE: 12/30/87 REVISED: COMPLETED:
ANNUAL REPORT - USEPA REPOSITORY FOR TOXIC AND HAZARDOUS MATERIALS,
ACTIVITIES FOR CERCLA (FY 87)
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Page 57 of 65
CLIENT OFFICE 083
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
L104 TOXIC CHEMICAL TESTING f ASSESSMENT
A TEST METHOD DEVELOPMENT
02 ENVIRONMENTAL HAZARD ANALYSIS FOR TOXIC SUBSTANCES
PPA (S)
RC
PROJECT
PROJECT OFFICER
HAZARD ANALYSIS
A EMSL CINN
24 ENVIRONMENTAL HAZARD ANALYSIS FOR TOXIC SUBSTANCES
J. J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 10/01/80 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: To evaluate chemicals which are of specific interest to the regulatory
programs in the Office of Toxic Substances. Improving the quality of
scientific and technical assessment is a major goal of the Office of Toxic
Substances.
RATIONALE: Application of new or improved analytical methods to identify and
quantitate chemicals of concern in the environment and to develop reliable
methods for routine monitoring associated with regulatory reviews is an
important component of OTS regulatory programs. This research provides
biological and chemical methods for identification and quantification of
of chemical compounds being reviewed by OTS.
APPROACH: Sampling and analytical procedures used to identify and quantitate
the presence of chemical compounds in the environment and the biota will be
assessed to meet regulatory needs. "Application of immunochemical techniques
including monoclonal antibodies will be investigated. Influence of extraction
and clean-up procedures for multi-residue methods will be evaluated.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
Cornell University, under a cooperative agreement, has a project titled "The
Determination of Intractable Organic Compounds by Direct Liquid Injection
(DLI)/Thermospray Liquid Chromatography (LC)/Mass Spectrometry (MS) and
LC/MS/MS". The first budget period of the three-year cooperative agreement was
extended to September 1986. This first budget period was funded in FY 85 with
L104 research and development resources to investigate methods for azodyes. FY
87 funds are now available for continuation of this project.
ACCOMPLISHMENTS AND RESULTS TO DATE:
During the quarter a funding package was provided for a cooperative agreement
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Page 58 of 65
with Cornell University, and funds ($49K) were obligated (Item #2190[A]). The
principal investigator at Cornell began the search for a post doctoral research
associate.
Two requests for information on the analytical chemistry of several large
volume commercial chemicals were received from the Office of Toxic Substances,
and brief status reports were provided within one week in each case. A
presentation was made at the program review for the Chemical Testing and
Assessment Research Committee.
STATUS AND SCHEDULE OF DELIVERABLES-:
2190 DUE: 09/30/87 REVISED: COMPLETED: 09/25/87
REPORT ON ADVANCED ANLAYTICAL TECHNIQUES APPLIED TO MONITORING TOXIC
SUBSTANCES
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Paige 59 of 65
CLIENT OFFICE 083
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
L104 TOXIC CHEMICAL TESTING f ASSESSMENT
J SUPPORT
28 QUALITY ASSURANCE FOR TOXIC SUBSTANCES
PPA (S)
RC
PROJECT
PROJECT OFFICER
QUALITY ASSURANCE
A EMSL CINN
25 QUALITY ASSURANCE FOR TOXIC SUBSTANCES
JOHN WINTER
PHONE: 513-684-7325
A*****************************************
PLANNED START: 10/01/80 PLANNED END: 12/01/99
******************************************
PROJECT DESCRIPTION:
GOAL: Provide quality assurance to support implementation of sections 4 and 6
of the Toxic Substances Control Act
RATIONALE: Quality assurance support for biological monitoring networks is
needed to ensure their application to regulatory reviews. Such data are
needed to conduct various analyses including exposure and risk assessments
required under the Toxic Substances Control Act.
APPROACH: Quality control samples and calibration standards will be prepared
and distributed for chemicals of interest to the Office of Toxic Substances.
Traceability to NBS will be addressed under the current IAG.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
EMSL-Cincinnati is responsible for the production and distribution of quality
control (QCJ and performance evaluation (PE) samples and calibration standards
to support the quality assurance (QA) program of the Office of Toxic Substances
(OTS). Contracts were awarded to Bionetics, Inc., to produce and distribute QC
and PE samples, to Northrop Services, Inc., tc produce and distribute cali-
bration standards and to eight laboratories to serve as organic and inorganic
referee laboratories. The National Bureau of Standards (NBS) is on the second
year of an interagency agreement (IAG) to establish traceability for QA
products where practical.
ACCOMPLISHMENTS AND RESULTS TO DATE:
USEPA Repository for Toxic and Hazardous Materials (Harry Kolde): A total of
total of 20,400 ampuls were distributed to 420 USEPA, USEPA contract, state,
and local laboratories. Four 13C-polychlorinated biphenyl (PCB) calibration
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Page 60 of 65
standards were sent to OTS contractors.
Traceability to NBS (Ed Berg): A priority list of PE and QC samples requiring
NBS analyses was agreed upon. NBS will begin production of 3,000 sets of
PCB congeners for USEPA. Analyses have begun on selenium in fish samples.
QC Samples (Ray Wesselman): A total of 46,330 QC samples were distributed to
USEPA, USEPA contract, state, and local laboratories. To replace depleted
inventories, 24,000 ampuls of chlorinated hydrocarbon pesticides, PCBs, and
PCBs in oils were produced and analyzed.
STATUS OF LABORATORY OUTPUTS:
Output 1941[B] Annual Report on Traceability to NBS due 12/86 was completed.
Output 1941[C] Annual Report on Referee Analytical services due 12/86 was
completed.
STATUS AND SCHEDULE OF DELIVERABLES:
2094 DUE: 12/30/88 REVISED: 12/30/87 COMPLETED:
ANNUAL REPORT ON QC SAMPLE PROGRAM -- EPA REPOSITORY FOR HAZARDOUS
MATERIALS (FY 87)
1941 DUE: 12/30/86 REVISED: COMPLETED: 12/19/86
ANNUAL REPORT QC SAMPLE PROGRAM EPA REPOSITORY FOR TOXIC AND
HAZARDOUS MATERIALS (FY 86)
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Page 61- of 65
CLIENT OFFICE
FOURTH QUARTER STATUS REPORT" ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE
TITLE
BUDGET SUB-ACTIVITY
H109
INTERMEDIA
ISSUE
N
MANAGE THE MANDATORY QUALITY ASSURANCE PROGRAM
PPA (L)
01
MANAGEMENT SUPPORT FOR MANDATORY AGENCYWIDE QA PROGRAM
PPA (S)
MGMT SUPPORT FOR QA
RC
A
EMSL CINN
PROJECT
26
MANAGEMENT SUPPORT FOR MANDATORY AGENCY-WIDE QA PROGRAM*
PROJECT OFFICER
* *********
DANIEL
* * * * *
BENDER PHONE; 513-684-7301
***************************
PLANNED START: 10/01/86 PLANNED END: 01/01/00
***********>*******************************
PROJECT DESCRIPTION:
GOAL: To provide the Administrator with central management support for the
Mandatory Agency-wide Quality Assurance (QA) Program.
RATIONALE: It is necessary to develop policy, management procedures, and
technical procedures; coordination and direction for program implementation
and to review, evaluate, and audit Agency-wide QA activities in order to
respond to the QA mandate.
APPROACH: As the developmental mode proceeds to completion, the implementation
and evaluation activities will be increased via reviewing program plans,
conducting management system audits, reviewing QA audits, developing and
improving technical guidance, reviewing standard methods of various media,
assuring Agency allocation of adequate resources for Agency-wide QA programs,
and preparing a QA program status report for senior Agency management.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
PROJECT STATUS
This project is an ongoing support function involving the supplying of
technical and policy information and technical services such as document
review, committee and work group (WG) membership, document and report
preparation, and the coordination of the input into these activities of
regional, headquarters, and national laboratory Quality Assurance Officers
(QAOs).
ACCOMPLISHMENTS AND RESULTS TO DATE-
Policy statements concerning quality assurance (QA) positions of the Office of
Acid Deposition, Environmental Monitoring, and Quality Assurance (0ADEMQA) and
its four laboratories were written and/or reviewed. Progress toward the
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¦Page 62 of _65
implementation of these policies will be reported at an OADEMQA QAO meeting
during the first quarter of fiscal year (FY) 1988. Another round of regional,
national program office, and Office of Research and Development (ORD) QAOs
conference calls were held to plan for the national QAO meeting to be held in
early FY88,
Information concerning the routinely-used measurement method (RUMM) project was
sent to the contractor for the Section 518 report. The Physical and Chemical
Methods Branch performance in studies WP018 and WS020 was examined. An audit of
EMSL-Cincinnati sample handling and.analysis procedures of the pilot study for
the National Pesticide Survey was performed.
The regional QAOs were contacted regarding the responses of the Environmental
Services Divisions Director to a memorandum from the Quality Assurance
Management Staff (QAMS) concerning the RUMM project.
Reviews were made of manuscripts concerning management systems audits in ORD
laboratories, QA Program Plan Guidance, Intralaboratory Quality Control, Data
Quality Objectives in ORD laboratories, the LaMotte Residual Chlorine
Titratjon Kit as an alternate test procedure, and the EMSL-Cincinnati
recommendation for an analytical method for waters disinfected by chlorine
dioxide.
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
NO DELIVERABLES AVAILABLE
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Page 63 of 65
CLIENT OFFICE 070
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
******************************************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
N104 MULTI-MEDIA ENERGY
E UNDERSTAND & QUANTIFY AQUATIC EFFECTS
01 NATIONAL SURFACE WATER SURVEY
PPA (S)
RC
PROJECT
PROJECT OFFICER
NATL SURF WATER SRVY
A EMSL CINN
27 EVALUATION OF ANALYTICAL METHODOLOGY FOR ALUMINUM
J. LICHTENBERG
PHONE: 513-684-7306
******************************************
PLANNED START: 09/01/86 PLANNED END: 09/01/89
*** ***************************************
PROJECT DESCRIPTION:
GOAL: To evaluate existing methods used to determine the various species
of aluminum.
RATIONALE: The potential for aluminum toxicity is related to the chemical
form of aluminum present in freshwater systems. Current methods may not
be measuring the various species. Consequently, knowledge as to which
methods produce data for these species is needed.
APPROACH: Sampling and handling protocols and the existing methods will be
compared using developed standard reference materials and real world samples.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
This project appears in the work plans of the Environmental Monitoring Systems
Laboratory - Research Triangle Park (EMSL-Research Triangle Park) and an [A]
deliverable is listed in their Project Output Plan. The development and
evaluation of "Methods for the Analysis of Ions in Atmospheric Deposition" is
being conducted under a cooperative agreement with Illinois State Water Survey.
The contractor is responsible for acquiring available published methods and
delivering them to the Environmental Monitoring and Support Laboratory -
Cincinnati (EMSL-Cincinnati) for review prior to selection of methods for
evaluation and validation.
EVALUATION OF METHODS FOR REACTIVE ALUMINUM--(Item #2111[A]) The second
year funding package was prepared for the cooperative agreement to evaluate
methods for determination of aluminum in acid rain.
Jack Pfaff attended the ASTM meeting of ASTM Committee D-22 on Atmosphere (Acid
Rain) in Cincinnati on April 29-30. He chaired the discussion on the method to
determine calcium, magnesium, potassium, and sodium by atomic absorption in acid
rain samples.
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Page 64 of 65
STATUS OF LABORATORY OUTPUTS: None.
STATUS AND SCHEDULE OF DELIVERABLES:
2111 DUE: 12/30/87 REVISED: COMPLETED:
REPT ON EVALUATION OF ALUMINUM SPECIATION USING SYNTHETIC AND NATURAL
SAMPLES
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Page 65 of 65
CLIENT OFFICE 070
FOURTH QUARTER STATUS REPORT ON FY'87 PROJECT DESCRIPTIONS
Office of Acid Deposition, Environmental Monitoring and Quality Assurance
EMSL CINN: JULY - SEPTEMBER 1987
********************** ** ******************
CODE TITLE
BUDGET SUB-ACTIVITY
ISSUE
PPA (L)
N104 MULTI-MEDIA ENERGY
E UNDERSTAND & QUANTIFY AQUATIC EFFECTS
08 EFFECTS OF SNOWMELT & STORM EPISODES ON SURFACE WATER ACIDIF
PPA (S)
RC
PROJECT
PROJECT OFFICER
EPISODIC RESEARCH
A EMSL CINN
28 EFFECTS OF SNOWMELT AND STORM EPISODES ON SURFACE WATER
CORNELIUS I. WEBER
PHONE:
-684-7337
******************************************
PLANNED START: 10/01/86 PLANNED END: 09/30/92
******************************************
PROJECT DESCRIPTION:
GOAL: Provide information on the effects of acidification of surface
waters during snowmelt and storm episodes on fish and other aquatic life.
RATIONALE: Episodic acidification of surface waters from snowmelt and
storms has been cited as a major factor affecting aquatic organisms.
Although considerable data exist regarding the extent of chronic acidifi-
cation, there are very little data as to the extent, magnitude, duration
and frequency of short-term, episodic acidification in potentially sensitive
regions, and the biological effects of episodic acidification.
APPROACH: Field and laboratory studies of the aquatic biology effects of
episodic acidification on fish and other aquatic life will begin in FY-87 at
single site in New England or the mid-Atlantic states. Toxicity tests of
surface waters containing acidic runoff from manipulated catchments will be
performed with indigenous and/or surrogate fish and invertebrates on-site in a
mobile laboratory and in at EMSL-Cincinnati. Field studies of the standing
crop, species composition, and condition of indigenous fish and invertebrates
will be carried out prior to and following application of acid materials to
experimental catchments. Biological efects will be related to pH, aluminum,
calcium, alkalinity and other relevant water quality parameters.
PROJECT STATUS AND ACCOMPLISHMENTS TO DATE:
A contract has been awarded for development of the detailed work plan for the
field bioassay portion of the Episodic Response Project.
STATUS OF LABORATORY OUTPUTS: None
STATUS AND SCHEDULE OF DELIVERABLES:
2184 DUE: 08/30/87 REVISED: 09/30/87 COMPLETED: 09/28/87
FIELD BIOASSAY PLAN FOR THE EPISODIC RESEARCH PLAN (ERP) (TO OADEMQA)
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