QUARTERLY
PROGRESS
REPORT
PACIFIC NORTHWEST
WATER LABORATORY
CORVALLIS, OREGON
JULY1 —SEPTEMBER 30,1971
ENVIRONMENTAL PROTECTION AGENCY
ENVIRONMENTAL PROTECTION AGENCY
NATIONAL ENVIRONMENTAL RESEARCH CENTER
200 S.W. 35th STREET
CORVALLIS, OREGON 97330


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PACIFIC NORTHWEST WATER LABORATORY
QUARTERLY REPORT
July 1 through September 31, 1971
Environmental Protection Agency
National Environmental Research Center
200 S.W. 35th Street
Corvallis, Oregon 97330
October 1971

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CONTENTS
NATIONAL THERMAL POLLUTION RESEARCH PROGRAM 		1
NATIONAL COASTAL POLLUTION RESEARCH PROGRAM 		8
NATIONAL EUTROPHICATION RESEARCH PROGRAM 		17
NATIONAL WASTE TREATMENT RESEARCH PROGRAM 		23
CONSOLIDATED LABORATORY SERVICES PROGRAM 		37

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NATIONAL THERMAL POLLUTION RESEARCH PROGRAM
PPB 1613
Status of Projects and Significant Accomplishments
Work Plan ZBA: Improvement in Evaporative Cooling Methods
Dr. Garton is continuing literature survey and in-house research
on constituents, effects, and control of cooling tower blowdown.
Laboratory experiments are being conducted and initial results
have been obtained for effects of constituents in cooling tower
blowdown on algae, using Selenastrum capricornutum, and for fish
using juvenile steelhead for test organisms.
Work Plan ZFD: Heat Transport and Behavior in Large Hydrologic Systems
Meteorological and stream data collected during the 1969 study
on the Little Deschutes River were analyzed to determine the evaporative
heat loss. Energy budget computations failed to provide accurate
evaporation rates due to insufficient information on stream travel
time and inadequate stream temperature measurements. The final report
for this project is being prepared.
Work Plan ZFF: Engineering and Cost Aspects of Heat Dissipation
Environmental thermal testing of a simulated reflective particle
pond was completed. Draft of a working paper covering the reflective
pond investigation has been prepared.

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Work Plan ZFC: Heat Transport and Behavior in the Mixing Zone
A comprehensive workbook on mixing zone problems was initiated
early this summer. The objective is to compile results of in-house,
grant, contract, and outside sources into computer programs and
readily usable charts for the prediction of plume trajectory and
temperature for cooling water discharges. The book will be published
in two parts. The first part will be devoted to submerged outfalls
and the second part to surface discharges.
The first part is sixty percent complete. All charts and
correlation plots for no-current and stratified ambient conditions
for single or multiple diffuser outfalls have been completed.
Experimental data on crossflow and co-flow jets in ambient currents
are available for developing the remaining portion of the first
part. Analytical methods are being explored to fill the gap between
crossflow and co-flow discharges to complete the picture.
Plans are made for providing experimental data to check these
analytical models. A towing channel designed for this purpose is
now nearly completed. Assembly of the parts will begin within two
weeks at the Fluid Dynamics Laboratory, Pacific Northwest Water
Laboratory.
Work Plan ZFI: Advanced Power Generation
Inacti ve.

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Work Plan ZFD: Heat Transport and Behavior in Large Hydrologic
Sys terns
"Heat and Water Vapor Exchange between Water Surface and
Atmosphere," Cornell University, 16130 DIP, has been printed and
two-hundred copies received by NTPRP.
"Controlling Thermal Pollution in Small Streams," 16130 FOK,
Oregon State University is continuing.
Work Plan ZFJ: Beneficial Uses of Heated Water
The 1970 Annual Report of our grant with Eugene Water and Electric
Board, 16130 EIK, "Thermal Water Demonstration Project," was received.
Request for grant continuation was reviewed and continued support
highly recommended as the more productive phases of the project are
forthcomi ng.
Consulting Services
Work Plan ZFL: Consultation and Advisory Services
Mr. Christianson attended a meeting in Washington, D.C., for
technical review of work done under the Industrial Waste Studies
Program. A summary presentation was given on the Steam Generation-
Power Industry category. Comments and suggestions were offered by
the review group, which consisted of consultants and numerous EPA
personnel. Work progressed throughout the quarter on the draft
final report and reference guides covering this industrial category.
Assistance was provided Region VII in their evaluation of
Union Electric's proposed Rush Island power plant. Mississippi

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River temperature records were analyzed by Rainwater and Christianson
to determine critical periods with respect to anticipated operational
and discharge characteristics of the plant. Suggestions were made to
enable compliance with water quality standards.
Dr. Tichenor presented testimony on the cost of alternative
cooling systems for thermal power plants near Lake Michigan at two
public hearings: Wisconsin Department of Natural Resources, Madison,
Wisconsin, August 13, and Indiana Stream Pollution Control Board,
Indianapolis, Indiana, August 23.
Mr. Rainwater presented testimony in the form of a Statement
before the State Water Resources Control Board of California regarding
water temperature standards for the State.
Information was provided the Assistant Administrator for Research
and Monitoring on EPA-AEC relationships in thermal research and
Mr. Rainwater traveled to Washington, D.C., to attend a meeting
between EPA staff and representatives of AEC regarding future thermal
research activities and designation of R&D responsibilities -- EPA
and AEC.
Mr. Rainwater provided the Temperature Standards Work Group with
comments on New York State water quality standards, thermal criteria.
Information was provided all EPA regions on the subject, "Environ-
mental Impact of Nuclear Power Plants," in connection with the July 23,
1971, decision of the U.S. Court of Appeals of the District of Columbia,
in the case of the Calvert Cliff's Coordinating Committee vs the Atomic
Energy Commission.

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Mr. Rainwater and Dr. Tichenor met with staff of the Division
of Meteorology, Air Pollution Research, EPA, Research Triangle Park,
North Carolina, to coordinate air and water research relative to
electric power generation. Four interfaces and/or areas of potential
coordination were identified and personal contacts established.
Reports, Papers, and Presentations
1.	Dr. Shirazi attended the Annual Specialty Conference of
ASCE Hydraulic's Division, held at Iowa Institute of Hydraulic Research.
While there he met with Dr. McQuivey, USGS, to discuss the final
report of the EPA-USGS cooperative study.
2.	Mr. Rainwater presented a paper, "Recent Developments in
Thermal Waste Control," to the Intersociety Energy Conversion
Conference, Boston, Massachusetts, August 3-6, 1971,
3.	Larry Winiarski completed the final draft of a working paper
covering the Reflective Pond investigation.
4.	Dr. Shirazi was a guest speaker at a meeting of the Willamette
Chapter of ASME. The subject of his talk was dry cooling towers.
With cooperation from the Training Branch of the Laboratory, he
arranged for a tour of the Pacific Northwest Water Laboratory.
5.	Dr. Shirazi submitted a paper, "Dry Cooling Towers for Steam
Electric Power Plants in Arid Regions," for presentation at the Sixth
Conference of the International Association on Water Pollution Research
to be held in Jerusalem, Israel, June 1972.

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Plans for Second Quarter, FY 1972
Work Plan ZBA: Improvement in Evaporative Cooling Methods
Continue work on toxicity of blowdown from cooling towers. Dr.
Garton will be preparing a paper on the subject for presentation to
American Institute of Chemical Engineers, Dallas, Texas, February 1972.
Work Plan ZFL: Consultation and Advisory Services
Complete work on Reference Guide and final report on steam
generation for Industrial Waste Studies Program.
Work Plan ZFC: Heat Transport and Behavior in Mixing Zone
Continue analytical phase of workbook on mixing zones. Shakedown
equipment and Instrumentation for flume studies.

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NATIONAL COASTAL POLLUTION RESEARCH PROGRAM
PPB 1607
Status of Projects and Significant Accomplishments
Work Plan DBH: Estuarine and Ocean Outfalls
Selection of Safe Sites for Discharge. A draft of a report on
oceanographic sampling methods in nearshore waters was completed.
The report relates coastal oceanographic effects to the fate of
marine discharges.
Two two-dimensional models of current structure were run with
an eye to evaluating their efficacy in coastal pollution problems.
For a small grid (5x10) approximately one minute of CDC 3300 (CPU
plus print) is required to simulate one hour of 'real' time with
a 1 1/2 minute time step. The results of such purely numerical
experimentation and the input requirements of the models will be
incorporated as a part of a larger report on coastal oceanography.
ERTS Study. A joint proposal (EPA and Bendix Corporation)
on utilization of the Earth Resources Technology Satellite (ERTS)
(NASA) in coastal oceanography was submitted to NASA for consideration.
At this writing no awards have been announced.
Work Plan ZAH: PCB, Biocides, and Other Persistent Organic Compound
Distributions and Fates in Coastal Waters
A Finnegan gas chromatograph/mass spectrometer (GC/MS) has been
delivered. An organic chemist was hired and attended GC/MS operational

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training courses at San Jose, Athens, and Cincinnati. Current
efforts are centered around setting up lab facilities to conduct
research on persistence, accumulation, and distribution of harmful
organics in the marine environment.
Work Plan XAA: Barge Dump Disposal of Wastes in Ocean and Coastal
Waters
A polarographic system is being assembled to analyze for
extremely low concentrations of lead and other materials in ocean
and coastal waters.
Analysis of other trace materials (Hg, Cd, Se, As) continues
by computer-based analysis of Ge(Li) spectra taken by a multichannel
Pulse Height Analyzer (PHA) available to the Program at the Radiation
Center of Oregon State University approximately 2 to 3 days per month.
Work Plan ZAI: Mercury, Cadmium, Arsenic, and Other Heavy Metal
Distributions and Fates in Coastal Waters
A mercury uptake estimation experiment was conducted using
radioactive mercury in the environmental simulator at the Marine
Science Center, Newport, Oregon. The subjects during a ten-day
203
exposure to O.ly gm Hg (Hg )/gm water in a 25 1 tank were algae,
oysters, clams, and Dungeness crabs; preliminary results indicate
that the algae and/or bacteria took up Hg rapidly.
Grant and Contract Research
Work Plan DBH: Estuarine and Ocean Outfalls
16070 FJV: Coastal Pollution: Literature Search, Indexing
and Abstracting. At monthly intervals abstracts of papers relevant

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to coastal pollution are submitted by the grantee (Pollution Abstracts,
Inc.) to the Water Resources Scientific Information Center, and to
headquarters, NCPRP. WRSIC publishes the abstracts in its twice-
monthly publication, Selected Water Resources Abstracts, and NCPRP,
upon receipt of the abstracts, forwards copies to laboratories and
regional offices of EPA for immediate use.
16070 DGY: Dispersion in Hydrologic and Coastal Environments.
The grantee has begun writing the final report for this project. This
is expected to be finished during the next quarter. The reports
received during the quarter prepared by the grantee and his staff
are listed under "Reports, Papers and Presentations."
Work Plan XCF: Tidal Flats Influence on Estuarine Water Quality
16070 DG0: Tidal Flats in Estuarine Water Quality Analysis.
Laboratory studies were continued at Oregon State University in order
to better understand sulfate reduction. The results indicate a more
rapid decomposition of organics than had previously been antacipated.
Field measurements of sulfate profiles within the deposits will be
conducted during the next grant period. In addition, the use of
S-35 to better measure sulfate reduction over a short period of time
is being investigated.
Work Plan XAA: Barge Dump Disposal of Wastes in Ocean and Coastal
Waters
16070 EKZ: Oxidation of Organic Matter in the Seabed. The
University of Washington continues its work on the second year of
the grant. The grantee made two more cruises to Puget Sound to

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increase its coverage to 36 stations. The additional stations close
to population centers of Seattle and Tacoma did not show higher
rates of oxygen uptake than previous stations, but intertidal
stations at the mouth of the Snohomish River in Everett had rates
of uptake as high as four times the highest rates ever measured
in Puget Sound. Dr. Pamatmat, the principal investigator, will go
back to this location in two weeks to repeat the measurements at the
same stations and go farther up the river for in situ measurements
on the riverbed. The long-term laboratory experiment on organic
matter oxidation was begun recently.
Proposals Sought
Mr. Gil Jaffe, Director of the National Oceanographic Instrumen-
tation Center, visited the Program Chief to discuss a proposal for
the NOIC to evaluate equipment and instruments designed for coastal
pollution measurements. The grant would cover basically two activities:
1.	direct laboratory or field evaluation of specific items of
equipment recommended for study by our program, and
2.	collaboration between NOIC and NCPRP staffs on evaluation
of research and demonstration projects awarded to a third party.
Consulting Services
Work Plan ZAR: Technical Consultation on Coastal Pollution Problems
1. Dispersal of Reserve Mining Taconite Tailings Waste in Lake
Superior. Actual field work commenced on July 26. Four current

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meter stations were established and sediment traps set up at two
positions. Equipment was forwarded to the Duluth site for use in
investigating turbid water areas. Primary measurements utilized
horizontal and vertical transmissometer transects, backed up with
dye traces and water samples.
Official assignment of the responsibility to NCPRP for conduct
of the Lake Superior taconite tracing study (under the Great Lakes
Region) was received September 14. Four employees were subsequently
deployed for temporary duty in the Lake Superior area, two to remain
until the completion of the study. A Coulter counter was taken to
the site to analyze samples immediately after receipt, and a one-
week cruise on the Coast Guard Cutter WOODRUSH provided samples for
study. An investigation to determine resuspension of settled tailings
fines began in our laboratory, and we initiated a review of the
basic data and computations used by Reserve Mining to estimate the
volume of their waste in the delta and on the bottom of the lake.
Recent studies have concentrated on tracing the layer of turbid
water near the bottom of the lake. Proprietary transmissometers
performed very poorly during the field studies. The sediment traps
built especially for this study performed very well.
In addition to field measurements on tailings transport,
detailed analytical work on noxious trace elements (Cd, As, Se, Hg)
is being conducted by neutron activation analysis at Corvallis.
2. Three conductivity meters were loaned to the Oregon
Institute of Marine Biology (of the University of Oregon) in
Charleston for its work in Coos Bay.

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3.	At the request of the Biological Methods Manual Committee,
the Program Chief prepared a critique of the bioassay section of the
proposed manual. A staff member attended a three-day meeting in
Denver for the purpose of reviewing the final draft.
4.	Chief, Chemical and Biological Oceanography, attended a
second meeting in Washington, D.C., of the committee to prepare a
request for proposals on the "Fate and Persistence of Oil in Marine
Waters." The draft RFP was later reviewed by NCPRP personnel and
extensive revisions made for the final form.
5.	Mr. Vic McCauley, EPA headquarters, requested an outline
of our capabilities for analysis of marine samples. He was provided
a list of parameters which can be routinely analyzed in our laboratory
and a list of specialized equipment and analytical capabilities
which are not routinely employed for marine analysis, but which
can be used for special purposes.
6.	Program Chief attended the Summer Study on the Environmental
Quality Program of the International Decade of Ocean Exploration
at Durham, New Hampshire. The study was organized by the National
Scientific Committee on Oceanography with the endorsement of the
Ocean Affairs Board.
Reports, Papers and Presentations
1. "Applications of Some Numerical Models to Pacific Northwest
Estuaries," by R. J. Callaway, was published in the Proceedings,
1971 Technical Conference on Estuaries of the Pacific Northwest,
Circular #42, Engineering Experiment Station, Oregon State University,
pp. 29-97.

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2.	Dr. Baumgartner presented a paper entitled, "Capabilities
and Limitations of Models as Estuarine Pollution Control Techniques,"
at the 7th Annual Conference and Exposition of the Marine Technology
Society meeting in Washington, D.C., August 1971.
3.	A report by Baumgartner, D. J., M. H. Feldman, and Carl L.
Gibbons, "A Procedure for Tracing of Kraft Mill Effluent from an
Ocean Outfall by Constituent Fluorescence," was published in Water
Research, Volume 5, Number 8, pp. 533-544.
4.	Papers from grant 16070 DGY, Cal Tech:
Sullivan, Paul J., "Some Data on the Distance-neighbour Function
for Relative Diffusion"
Di tmars, John D., "Mixing of Density Stratified Impoundments
with Buoyant Jets"
List, E. J., "Laminar Momentum Jets in a Stratified Fluid"
Sullivan, Paul J., "The Penetration of a Density Interface by
Heavy Vortex Rings"
Cederwall, Klas, "A Float Diffusion Study" and "Buoyant Slot
Jets into Stagnant or Flowing Environments"
Okoye, Josephat K. , "Characteristics of Transverse Mixing in
Open Channel Flows"
Plans for Second Quarter, FY 1972
Work Plan DBH: Estuarine and Ocean Outfalls
Work will continue on the evaluation of numerical models of
drift flow. A small example area will be chosen for illustration,
as well as several idealized cases. Evaluation of self-contained,

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submerged tide recorders for offshore work will be initiated. These
instruments are required in order to properly specify the incoming
tidal wave in the model.
Work Plan ZAH: PCB, Biocides, and Other Persistent Organic Compound
Distributions and Fates in Coastal Maters
We will continue setting up lab facilities and begin study of
transport, accumulation, and interchange mechanisms. Initial
investigations will be made to determine present general levels of
PCB, DDT, DDE, and other persistent organics in the marine environment.
Work Plan ZAR: Technical Consultation on Coastal Pollution Problems
The Lake Superior taconite tracing study will be completed and
the data from the study presented at the Lake Superior enforcement
conference.
General
The Director, National Environmental Research Center, requested
a list of research programs which would expand the scope of our
program's studies, or which are directed to studies of other
elements of the environment as air pollution. Program Chief
suggested future studies on:
1.	pollution interference with pheromone identification by
marine organisms;
2.	development of autopsy and biopsy procedures for marine
fish, shellfish, and marine birds;
3.	demonstration of available marine bioassay tests to prevent
the discharge of unsatisfactory treated wastes;

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4.	ecosystem assessment techniques;
5.	deep sea disposal of continental wastes;
6.	effects of accumulated pollutants in the Gulf of Mexico;
7.	air-sea interchange in major coastal pollution problem areas;
8.	health aspects of municipal sewage discharge in coastal
regions;
9.	establishment of case studies group;
10.	remote sensing; and
11.	a concentrated program to solve the pollution problem in
the New York Bight.
Presidential interns in science and engineering, sponsored by
the National Science Foundation, were requested for future studies on:
1.	behavior and fate of mercury compounds in the marine
envi ronment;
2.	behavior of pesticides and other persistent chlorinated
hydrocarbons in the marine environment; and
3.	case studies of causes of pollution in New York Harbor
and research needs to effect recovery of water quality.

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Work Plan ZCJ: A Comparative Study of Eutrophication in Two
Recreational Mountain Lakes
Limnological studies were continued on Diamond Lake on a
monthly basis. Carbon-14 primary productivity measurements were made
at the deep hole location at two-week intervals. The lake is decidedly
eutrophic, with dissolved oxygen values less than 2 mg/1 and
significantly increased levels of dissolved phosphorus and ammonia in
the hypolimnion. Extensive littoral weed growth was observed. A
dense diatom bloom occurred in early June, followed later in the
summer by heavy growths of blue-green algae. Very dense populations
of zooplankton, predominantly Daphnia, were sampled in July. The
bottom fauna is dominated by midge larvae. The regular sampling
program was terminated the last week of September owing to the onset
of winter at that elevation. Seven groundwater observation wells were
installed at various locations around the lake. Some intermittent
sampling of the lake is planned for the winter months.
The Waldo Lake groundwater study was resumed in July. Water
levels in the observation wells were low, indicating no discharge
to the lake. Residual rhodamine dye (injected last year into the
septic field) was found at a number of wells, demonstrating poor
flushing characteristics of the aquifer and suggesting that local
buildup of septic tank effluents may be occurring.
A limnological survey of Waldo Lake proper was made in August,
following the procedures observed during the past two years. It
is intended to so survey the lake during August of each year for some
time to come.

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Work Plan ZCH: New Methods for the Removal and/or Inactivation of
Nutrients in Lakes
At Cline's Pond, monitoring of the sodium aluminate inactivation
experiment continued. An Anabaena bloom of short duration was
followed by return of pH values to about 7 and increase of Secchi
disc transparency to 1.4 m. Dissolved oxygen below 1.5 m is quite
low. Review of the data indicates lower total phosphorus, chlorophyll
a_, dissolved iron, dissolved manganese, ammonia, and total Kjeldahl
nitrogen than during pretreatment years. The water transparency and
pH have been more stable than in previous years. The large Daphnia
and tadpole populations which appeared in past years have not appeared.
Phosphorus in Shagawa Lake sediments is being studied using
various leaching solutions including ammonium chloride, sodium
bicarbonate, ammonium acetate, and dilute sulfuric-hydrochloric acid.
Eighteen lakes throughout Oregon were visited as part of a
survey to locate further potential study sites. Water samples were
taken for characterization and evaluation of each lake. A report
will be completed during the next quarter.
Work Plan ZBP: Lake Restoration
The established sampling program at the Shagawa Lake Project
was continued throughout the quarter. Concentrations of ortho and
total phosphorus and Chlorophyll a_ during July of this year were
generally the same as those during the corresponding time last year
at Shagawa Lake. Unlike previous years, the Aphanizomenon bloom

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has not yet developed this summer. Recording flow gages are now in
operation in "Stinky Ditch," the secondary treatment plant outfall,
and the raw wastewater by-pass ahead of the treatment plant.
Automatic samplers are also in operation at the latter two positions.
Development of a mathematical model for Shagawa Lake has
conti nued.
Other Activities
Arnold Gahler attended the national meetings of the American
Chemical Society on September 13-17.
Charles Powers attended an EPA-sponsored meeting in Rochester,
NY, September 8, concerning EPA participation in the International
Field Year--Great Lakes.
Kenneth Malueg attended a meeting at the EPA Regional Office,
Atlanta, to discuss plans for the restoration of Lake Apopka.
Earlier he visited lake restoration sites in Sweden.
Publications and Presentations
Donald Schults presented a seminar on lake restoration to the
Corps of Engineers at Walla Walla, Washington.
Grant and Contract Research
The following research grant and contract proposals were reviewed.
1.	"The Control of Predominating Algal Species"
2.	"Septic Tanks and Lake Eutrophication"
3.	"Study of the Interaction between the Blue-Green Alga,
Plectonema boryanum, and its Cyanophage"

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4.	"Feasibility of Eutrophication Control in Lake Arlington
by Reducing Nutrient Input"
5.	"Removal of Algae from Eutrophic Lakes and Oxidation Ponds
by Polymer-Aided Centrifugation"
6.	"Algal Nuisance Control by Aeration"
7.	"Restoration of Lake Lansing, Meridian Township, Ingham
County, Michigan"
8.	"The Nutritional Ecology of Nuisance Aquatic Plants"
Plans for Second Quarter, FY 1972
Physiological Control
Conduct training course in algal assays.
Continue nationwide algal assay lake survey.
Continue Snake and Columbia River system assay study.
Complete fall quarter Shagawa Lake assay series.
Conduct regular seasonal assays on Cascade and coastal lakes.
Technology Development
Complete data analysis and write reports for Cline's Pond and
Diamond Lake projects. Continue routine monitoring of Cline's Pond.
Conduct further nutrient exchange studies on Diamond Lake
sediments.
Complete report on groundwater studies at Waldo Lake, and on
the survey of new study sites.
Participate in IFYGL - Lake Ontario planning.

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Lake Restoration
Begin construction of AWT plant at Ely, Minnesota.
Continue existing projects.

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NATIONAL WASTE TREATMENT RESEARCH PROGRAM
FOOD WASTES RESEARCH - PPB 1206
Status of Projects and Significant Accomplishments
Work Plan ZAQ: Technical Consultation and Data Dissemination
The Second National Symposium on Food Processing Wastes proceedings
was reproduced and dissemination was initiated.
At the request of the Seattle Regional Office, Mr. Dostal
consulted with EPA Regional personnel and personnel from Oregon's
Department of Environmental Quality on potato processing and waste
treatment.
Mr. Dostal met with key members of the National Canners
Association (NCA) and discussed both ongoing and needed research
in the fruit and vegetable area.
A full-scale waste treatment system, first of its kind on a
salmon cannery, was visited by Mr. Boydston and Mr. Dostal.
Work Plan FGF: Grant Monitoring
Mr. Dostal and Mr. Thompson visited Del Monte Corporation's
Plant No. 3 in San Jose, California, (Grant 12060 HFY) and NCA's
laboratory in Berkeley, California (Grants 12060 PAV and 12060 EDK).
Widmer's Wine Cellars, Inc. in Naples, New York, (Grant 12060
EUZ) was visited by Mr. Taylor and Kent Cheese Co. in Kent, Illinois,
(Grant 12060 EKQ) was visited by Mr. Cochrane. Corn Products Co.
in Pekin, Illinois, (Grant 12060 DPE) and American Distilling Co.
in Pekin (Grant 12060 FLL) were visited by Mr. Taylor and Mr. Cochrane.

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Messrs. Dostal, Burm, and Taylor spent a week in EPA Headquarters
assisting in a review meeting on the industrial "Reference Guides".
Prior to the meeting a "Reference Guide" was developed internally
for the Beet Sugar Processing industry.
Two new grants were awarded:
12060 HCW, Anheuser, Busch, Inc., Houston, Texas
Project Officer - R. Hi Her
12060 HPC, California Department of Agriculture Wine Advisory
Board, Fresno, California
Project Officer - D. W. Taylor
The following grant proposals and preproposals were received
during the quarter:
1.	Seafoods processing wastewater characterization.
2.	Rum distillery waste treatment by anaerobic digestion.
3.	The development and operation of an acid whey fermentation,
demonstration pilot plant.
4.	Reduction of waste flow and organic load from the blanching
process in vegetable canning.
5.	Zero-effluent cane cleaner.
6.	Treatment of total wastes from wet milling industry and
development of economical water reuse techniques.
7.	Technological survey, wastewater and sludge reclamation/reuse
in the frozen food industry.
8.	Waste treatment facilities for Gold Seal Vineyards.

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Work Plan GJJ: New and Improved Treatment Processes for Food Processing
Wastes
The full-scale anaerobic trickling filters which treat starch plant
waste at United Centennial Mills, Spokane, Washington, were monitored
until August 20 at which time an explosion and fire in the processing
plant halted production. On September 15 the processing plant was
placed into operation. BOD and COD removals declined to about 60 percent
prior to the explosion. This was probably due to the large amount of
suspended solids in the influent stream, over 2,000 mg/1.
Work Plan GKI: Secondary Treatment Processes for Highly Seasonal
Wastes
A pilot RBC unit containing 30 discs, two feet in diameter,
was started on a waste made from dehydrated potato granules. The
artificial waste is being used so that the hydraulic and organic
loadings can be varied independently. Initially the hydraulic
loading was set at 0.25 gpd/sq ft with a COD concentration of 1000
mg/1. Thus far, periodic sloughing of the biomass has resulted in
wide removal ranges for both SS and COD, from 30 to 90 percent.
Grant and Contract Research
The current status of monitored grants is described below.
These grants are in the process of being assigned to work plans.
1. 12060 EUZ: Winery Wastewater - Characterization and
Treatment. Most of the mechanical' problems have been taken care
of and the post-construction studies are being initiated.

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2.	12060 EKQ: Kent Cheese Company - Waste Treatment Facility.
Post-construction studies are about 80 percent complete. Effluent
BOD has been in the 20 to 40 mg/1 range with percentage reductions
consistently over 95.
3.	12060 FLL: Activated Sludge: Bio-Disc Treatment of Distilling
Wastes. Construction has been completed and the post-construction
studies are just being initiated.
4.	12060 EHS: Cannery Waste Treatment by Lagoons and Oxidation
Ditch. The final report has been revised and retyped for submittal.
5.	12060 EHV: Aerobic Secondary Treatment of Potato Processing
Wastes with Mechanical Aeration. The final audit has not been
done.
6.	12060 DSI: State-of-the-art, Sugarbeet Processing Waste
Treatment. Following revision, the final report was submitted for
reproduction.
7.	12060 ECF: Water Pollution Abatement in the United States
Seafood Industry-State-of-the-art. Will be completed after a final
audi t.
8.	11060 EZR: Complete Aerobic Treatment of Combined Domestic
and Industrial Wastes with Mechanical'Aeration. The final report was
submitted last quarter and is awaiting reproduction.
9.	12060 EIG: Full-scale Demonstration and Evaluation of Potato
Dry and Wet Caustic Peeling Processes. The project was extended so
that data could be collected during the first half of the 1971-72
processing season.

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10.	WP-01486-01: Status and Research Needs-for Potato Waste
Waters. Grant still not officially terminated.
11.	12060 FAD: Aerobic Treatment of Fruit Processing Wastes.
Questions raised by final audit have not been settled.
12.	WPD 93-04-68: Anaerobic-Aerobic Sugar Beet Waste Treatment.
Final report has been revised and is currently being typed
13.	12060 DXL: Reduction of Salt Content of Food Processing
Liquid Waste Effluent. Final report has been reproduced and
distribution has started.
14.	12060 EDK: Production and Disposal Practices for Liquid
Wastes from Canning and Freezing Fruits and Vegetables. Final
report is being revisedt
15.	12060 EHT: Use of Fungi Imperfecti in Waste Control.
Still waiting for an appendix of data.
16.	12060 EDZ: Pilot Plant Installation for Use of Fungi
Imperfecti on Vegetable Wastes. Revised final report is being typed.
17.	12060 FAK: Concentration of Sugar Beet Wastes for Economic
Treatment with Biological Systems. Revised final report is being
typed.
18.	12060 FQE: Dry Caustic Peeling of Tree Fruit to Reduce
Liquid Waste Volume and Strength. Final report submitted last
quarter to Project Reports System for reproduction.
19.	11060 FJQ: Pollution Abatement and By-Product Recovery
in Shellfish and Fisheries Processing - Phase I. Final report
submitted to Headquarters.

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20.	12060 EGU: State-of-the-art of Dairy Plant Wastes and
Waste-Treatment Systems. Draft of final report being revised.
21.	12060 FDR: Disposal of Rum Distillery Wastes. Termination
of project underway.
22.	12060 ESY: Improvement of Treatment of Food Industry Waste.
Final report still being revised.
23.	12060 EZP: Cannery Waste Treatment by the Kehr Activated
Sludge Process. Project not officially terminated.
24.	WPRD 151-01-68: Integrated Treatment of Liquid Wastes from
Food Canning Operations. Final report is being distributed.
25.	12060 EHU: Reconditioning and Reuse of Food Processing
Brines. Final report has been reproduced and is being distributed.
26.	12060 DQV: Removal and Recovery of Fatty Materials from
Edible Fat and Oil Refinery Effluents. Second draft of final report
reviewed.
27.	12060 DEQ: Elimination of Pollution by the Utilization of
Protein Concentrates (Dried Whey) from Milk Residues of Cheese Making.
Status still unknown.
28.	12060 DPE: Treatment of Wastes from the Wet Milling Industry.
Post-construct!'on studies just initiated.
29.	12060 DSB: Demonstration of a Full-Scale Waste Treatment
System for a Cannery. Final report submitted to Headquarters.
30.	12060 EAE: Evaluation of Controlled Temperature and Forced
Aeration in Trickling Filter Treatment of Food Canning Waste Waters.
Final report is being reproduced.

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29
31.	12060 DXF: Development and Demonstration of an Ultrafiltration
Plant for the Abatement of Pollution from Cottage Cheese Whey. Final
report is being reproduced.
32.	12060 FJK: Acid Emulsion Breaking - Activated Sludge for
Bakery Waste. Construction completed,
33.	12060 ESC: Separation, Dewatering and Disposal of Sugarbeet
Transport Water Solids. No activity until next beet sugar "campaign"
starts.
34.	12060 FUR: Membrane Separation of Soybean Whey for Product
Recovery and Waste Treatment. Testing continues.
35.	12060 FTC: State-of-the-art Study of Water Pollution
Control from the Beverage Industry. About 75 percent of the work
has been completed and the termination date was extended to
September 1, 1971.
36.	11060 DLF: Tertiary Treatment of Combined Domestic-Industrial
Wastes. Post-construction studies ended in August. Data analysis
and final report preparation are underway.
37.	11060 DJB: Controlled Treatment of Combined Potato
Processing - Municipal Wastes by Anaerobic1 Fermentation, Aerobic
Stabilization Process. Construction of facilities nearing completion.
38.	11060 DUJ: Dynamic Process Development for Biological
Treatment of Whey Bearing Wastes. Final report still being revised.
39.	11060 ENF: Vermont Cheese Industry Pollution Abatement.
Project has not been initiated.

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30
40.	12060 PAV: Low Water Volume Enzyme Deactivation of Vegetables
Before Preservation. Blanching runs have been done on asparagus,
peas, snap beans, corn, and beets. Thus far, the results from
the hot air blancher look very promising. The finished products
will be evaluated after a six-month holding period for vitamin and
mineral content.
41.	12060 HFY: Dry Caustic Peeling of Clingstone Peaches on a
Commercial Scale. The full-scale (15 ton per hour) "dry caustic"
peeling line was operated on clingstone peaches until September 24.
Peeling was satisfactory and the peel solids were kept out of the
liquid wastestream. Water use in the rinsing was about 1 gpm per
ton of fruit as compared to 11 gpm per ton of fruit on the
conventional line.
42.	12060 FRW: Water and Waste Management in Sweet Potato
Processing. The existing processing equipment and methods are
being evaluated for water usage and waste discharges prior to
implementing changes.
43.	12060 HCW: Submerged Combustion Evaporation System for
Concentration of Brewery Spent Grain Liquors. A submerged combustion
evaporation will be evaluated on its ability to concentrate spent
grain liquor from screening and pressing operations. Following
concentration the material will be dried with other grain, resulting
in a salable by-product.
44.	12060 HPC: Pilot Scale Treatment of Wine Stillage. Both
activated sludge and anaerobic trickling filter pilot plants will

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31
be operated on brandy stillage to develop design criteria and cost
estimates.
Consulting Services
Listed under work plan ZAQ.
Reports, Papers and Presentations
Listed under work plan ZAQ.
Plans for Second Quarter, FY 1972
1.	Continue grant monitoring.
2.	Monitor full-scale anaerobic filters.
3.	Continue work on development of effluent standards.
4.	Continue operation of RBC pilot plant.
PAPER AMD FOREST INDUSTRIES RESEARCH - 1204, 1210, 1301
Status of Projects and Significant Accomplishments
Work Plan GJG: Technical Consultation and Data Dissemination
Continued major efforts in this area concerned time spent on
Section 13 permit activities; aid to the Regional Office at Seattle
on waste discharge requirements for Scott Paper in Everett, Wash.,
and to the Chicago Regional Office. Mr. Scott attended the Standards
Development meeting in Washington, D.C.
Work Plan FCK: Coliform and Solids Removal from Aerated Lagoon
Effluent
This project has been completed and the data are being consoli-
dated for presentation at an AICHE meeting in Dallas, Texas. Efforts

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32
were .iade to determine the effect of adding polymers and flocculants
to aerated lagoon effluent to reduce waste concentrations in pulp
and paper plant discharges.
Dr. Knittel and a student assistant have joined the staff to
work on fecal coliform problems associated with pulp and paper
wastes. Their activities have centered on establishing a micro-
biological laboratory and starting culture techniques.
Work Plan GJH: Suspended Solids Removal by Mechanical Means
Construction has been completed on this field project at Crown-
Zellerbach's Lebanon mill. A test program has been set up and the
initial phase of treating settled waste in the aeration basin has
begun. Samples are now being taken and data collected on the
microscreen treatment of raw wastes. During the quarter a failure
of the side-hill screen prompted the installation of a tube settler
for use on the unsettled primary wastewater. Bob Shankland and
John Ruppersberger are expending a majority of their time on this
project.
Work Plan ZBI: Series Biological Treatment of Pulp and Paper Wastes
A bench scale multiple unit activated sludge treatment plant
has been set up to study treatment of sulfite pulping wastewater.
After startup operation certain modifications have been made to
obtain dependable results with the fibrous wastes. Supplementary
trials are being conducted with a synthetic sulfite waste using
dried SWL and acetic acid.

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Work Plan ZAX: Pollutional Aspects of Bark Leachate
The lysimeter collection of bark leachate has started again
with the fall rains. This project has studied leachate from four
different bark types over a full year period. Sampling will be
continued through the second winter.
Work Plan FCD: Grant and Contract Monitoring
Grant applications and research proposals reviewed during the
quarter related to the following subjects:
1.	Fate of Nitrogen Fertilizers in Forest Soil: Conversions,
Movement, and Losses.
2.	Effects of Fire on Lakes in a Natural Forest Ecosystem.
3.	Evaluation of Effect of Various Biological Treatment Processes
on Colored Organisms in Pulp and Paper Wastewaters.
4.	Development of a Process Control Computer System for a
Modern Pulp and Paper Mill Wastewater Treatment Plant.
5.	Organic Compounds in Pulp Mill Lagoon Discharge.
6.	A Test Method for Volatile Component Stripping of Wastewater
i n Cooli ng Towers.
7.	Factors Affecting Slime Accumulation in Hardboard Mill
Effluents.
8.	Analysis of Organic Compounds in Pulp Industry Wastewater.
9.	Summary Review of Recovery of Chemicals from Kraft, Sulfite,
and NSSC Pulping and Treatment of Waste Materials.

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34
Grant and Contract Research
Current status on grants monitored by Paper and Forest Industries
Research personnel.
1.	12040 DBD: Color and Mineral removal from Kraft Bleach
Wastes. In the final year of the program examining the use of various
ion exchange resins.
2.	12040 DEH: Studies of Low Molecular Weight Lignin Sulfonates.
At the completion of the last year of this seven year project, isolation
and characterization of certain fractions of sulfonates have been
accomplished. An extension of time has been allowed for the final
report.
3.	12040 DLQ: Slime Growth Evaluation of Treated Pulp Mill
Wastes. Now in the final report preparation for this investigation
which has shown that biologically treated pulp mill waste will not
support slime growths as well as untreated waste.
4.	12040 EEK: Treatment of Selected Internal Kraft Mill Wastes
in Cooling Towers. BOD of condensates can be reduced and cooling
water recycled reducing mill pollution load and water needs. Report
has been completed and is awaiting publication.
5.	12040 EEL: In-Plant Treatment of Dilute Wastes of the Pulping
Industry. The project has completed the fifth and final application
of reverse osmosis to unit process streams in the pulp and paper
industry. Report is being prepared.
6.	12040 EFC: Pollution Abatement by Fiber Modification. Final
report approved for publication. Laboratory study of strengthening
paper fibers by the addition of waste lignin sulfonates.

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35
7.	12040 ELW: Aerated Lagoon Treatment of Sulfite Pulping
Effluent. The final report has been approved for publication.
8.	12040 EMY: Evaluation of Role of High Rate Trickling Filters
and Aeration Devices Separately and in Combination for the Advanced
Biological Treatment of Integrated Kraft Pulp and Paper Mill Effluents.
The final report is being published.
9.	12040 ESV: A Demonstration Plant Evaluation of Four Methods
for Pulp and Paper Mill Sludge Utilization and Disposal. Separate
burning and log fuel addition burning studies continue. Soil
assimilation studies continue.
10.	12040 EXQ: Steam Stripping of Kraft Pulp Mill Effluent
Streams. Progress has continued to the end of the third and final
year. A time extension has been given for project completion and
write up.
11.	12040 FKS: Steam Stripping and Rectification of Kraft Mill
Condensates and Black Liquors for Pollution Control and By-Product
Recovery. Program alteration and plant design completed.
12.	12040 FUB: Closure of Water Use Loop in NSSC Pulp and Paper-
board Mill Utilizing Reverse Osmosis as a Unit Operation. Phase I
pilot operations completed. Report in preparation. Hold period of
3 months on decision for commercial plant. Project alteration
includes emphasis on recycle rather than R.O. treatment.
13.	12040 GQD: Coliform Growth and Control in Aerated
Stabilization Basin. A cooperative study will determine controlling
factors of coliform growth in aerated basins treating weak spent

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36
sulfite liquor and paper machine white water. Study initiated this
quarter.
14.	12040 HDU: Mercury Recovery from Sediments and Sludges.
Six mercury recovery techniques will be evaluated for treatment of
sediments, process sludges, and liquid wastestreams. The optimum
process will be used on a chlor-alkali plant effluent. Study
initiated this quarter.
15.	12100 EBG: Influence of Log Rafting on Water Quality.
Currently working on a four month extension, studies have shown
the effect of log handling and storage in water; a survey of practice
has been completed for the Northwest.
16.	13010 EGA: Studies on Effects of Watershed Practices on
Streams. This study of logging methods has been completed and the
final report submitted for publication.
Plans for Second Quarter, FY 1972
1.	Continue R&D grant and contract monitoring and review.
2.	Provide, on request, consulting services to Headquarters,
regional offices, and states.
3.	Continue with in-house project work.
4.	Initiate work on hardboard wastewater treatment.

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CONSOLIDATED LABORATORY SERVICES PROGRAM
Status of Projects and Significant Accomplishments
General
Final pieces of hardware for field gas chromatograph were
received during the last week of this quarter. Assembly and field
evaluation are programed for the next quarter.
The new SHAVES bookkeeping and bench sheet production program
is functioning efficiently. Scheduling of people for production
line operation has been simplified. The weekly summary alerts
the staff where resources must be applied to most efficiently
handle the samples.
Five people have left through resignation, retirement, or
transfer. They have been replaced by three new employees and a
returning temporary. Optimum productivity continued during personnel
changeover.
The analytical load peaked in the month of September, which
is a change from previous years where the peak was attained in the
month of August. Indications are that the load will continue to
increase through the next quarter.
Automated Analytical Systems
A dual sampling probe has been installed on the Technicon II
pump in the Technicon II system. This dual probe provides the
capability to poogram spiked samples and test samples into the system.

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Atomic Absorption Spectrophotometry
Minor problems still continue to occur with atomic absorption
spectrophotometric analysis. Instrument response- is sluggish after
measurement of high level of constituent. Restandardization is a
necessity after a high concentration has been aspirated through the
flame zone.
Problems occasionally arise when the dissolved concentration
of metal is greater than the total concentration. Studies are being
made to determine the source of this problem.
Specialized Analysis
PBI (Pearl Benson Index), arsenic, selenium, total solids,
dissolved solids, and suspended solids analyses have occupied the
attention of the specialized group this quarter. Some problems
have been encountered in the analysis of selenium in saline waters.
Analyses of selenium by neutron activation analysis have been
performed to check on colorimetric procedures.
Analysis of Carbon
Particulates exiting from the total carbon furnace and the
inorganic carbon furnace have been plugging the switching valve in
the Beckman 915 system. A simple modification consisting of
installation of a micron filter on the exits of both furnaces has
solved the problem of clogging in the switching valve. This
modification has resulted in less downtime of the instrument.
The quartz tubes for the inorganic furnace and the total carbon
furnace are being fabricated locally from a purchased supply of

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39
quartz tubing. The cost saving is appreciable over purchasing
commercial quartz combustion tubes.
Work continues to be done with the Oceanography International
Carbon System. A technician is being trained to permit for more
flexibility in the use of this system.
Kjeldahl Analysis
The backlog is once again beginning to increase in the Kjeldahl
section. Efforts are being made to reduce this backlog.
Computer Services
The Computer Services section concerned itself with thermal
pollution modeling, analysis of data from neutron activation
analysis system, and assistance to Eutrophication in reduction of
algal assay data. A small amount of effort was given to the
ecological modeling of Lake Shagawa — Ely, Minnesota.
Biology
A number of sampling trips was conducted for the Waste
Treatment Research Program. Routine samples were also analyzed
for the Eutrophication Research Program.
Microbi ology
The pollution surveillance program had the only input of routine
samples for microbiological analysis.
Areas of Concern
Planning and priorities still continue to be a source of concern
especially with the changing pattern of peak loads.

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Reports and Papers
None.
Plans for Second Quarter, FY 1972
1.	Continue efforts to reduce turnaround time and backlog in
the laboratory.
2.	Test in the laboratory the CC^, Ng, 0g gas analyzer and
then field test it.
3.	Resubmit paper on oil pollution.
4.	Complete paper on SHAVES.
5.	Continue efforts to produce the report on analysis of
total carbon, inorganic carbon, and particulate inorganic carbon,
and, also, particulate organic carbon.

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