QUARTERLY
PROGRESS
REPORT
PACIFIC NORTHWEST
WATER LABORATORY
CORVALLIS, OREGON
April 1—June 30 1972
ENVIRONMENTAL PROTECTION AGENCY
ENVIRONMENTAL PROTECTION AGENCY
NATIONAL ENVIRONMENTAL RESEARCH CENTER
200 S.W. 35th STREET
CORVALLIS, OREGON 97330

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*1 PRC**-

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PACIFIC NORTHWEST WATER LABORATORY
QUARTERLY REPORT
April 1 through June 30, 1972
Environmental Protection Agency
200 S. W. 35th Street
Corvallis, Oregon 97330
July 1972

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CONTENTS
NATIONAL EUTROPHICATION RESEARCH PROGRAM 		1
NATIONAL EUTROPHICATION SURVEY PROGRAM 		13
NATIONAL WASTE TREATMENT RESEARCH PROGRAM	16
NATIONAL THERMAL POLLUTION RESEARCH PROGRAM	29
NATIONAL COASTAl POLLUTION RESEARCH PROGRAM	37
CONSOLIDATED LABORATORY SERVICES PROGRAM 		44

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NATIONAL EUTROPHICATION RESEARCH PROGRAM
Status of Projects and Significant Accomplishments
During this quarter NERP personnel continued to spend a great
deal of its time assisting and consulting with the National Eutrophication
Survey Program (NESP).
Work Plan ZCB. Assay Procedures for Determining Productivity Responses
The second series of Lake Micnigan samples collected in April 1972,
are being assayed for their algal growth potential. Singular phosphorus
additives, ranging from 0.005 to 0.020 mg P/l, with and without the
addition of nitrogen, trace metals, and iron were made to the lake
water samples. Preliminary results obtained with Lake Michigan Sample
No. 8623 indicated that when phosphorus was added (ranging from 0.005
to 0.020 mg P/l) and the concentrations of the other nutrients kept
constant, there was a corresponding increase in algal productivity
ranging from 0.04 to 9.40 mg dry wt/1 . These data indicate that, at
the time and place of the collection of the sample, phosphorus was
algal growtn-limiting in the lake water.
The marine algal assay research centered around the determination of
the effects of various salinity and phosphorus concentrations upon
the growth response of Dunaliella tertiolecta. Initial results show
excel lent correlation of increases in biomass produced to increasing
concentration of both salinity and phosphorus (i.e., at 5 percent salinity
and .046 mg P/l, 15.3 rriy dry wt/1 biomass was obtained. At 35 percent
salinity and 0.370 mg P/l concentrations the yield of Dunaliella tertiolecta
was increased to 226 mg dry wt/1.
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Isolation of fresh water algae from the spring quarter sampling
trip of the coastal and Cascade lakes is almost completed. Evaluation of
the isolated algae as potential algal assay test organisms should begin
next quarter.
Work Plan ZCA: Determination of the Environmental and Nutritional
Requirements and Physiological Processes of Freshwater
and Estuarine Algae and Plants
Preliminary investigation of selected trace metal requirements of
three species of fresh water algae indicate the following approximate
concentration levels of mercury, zinc, copper and cobalt that are
algistatic to the growth of Selenastrum capri cornutum and Anabaena -
flos-aquae in both Gorham and AAP culture medium. No specific results
are available for Microcystis aeruginosa at this time. The following
table indicates the approximate levels of Hg, Zn, Cu and Co (in yg/1)
which were algistatic to the test algae.
uq Hq/1	uq Zn/1	ug Cu/1	ug Co/1
AAP GORHAM AAP GORHAM AAP GORHAM AAP GORHAM
Test Alga MEDIUM MEDIUM MEDIUM MEDIUM MEDIUM MEDIUM MEDIUM MEDIUM
Selenastrum
capri cornutum 50	70 90 >_ 350 60 140 > 120 > 250
Anabaena
fl os-aquae >30 >80 	 > 250 	 100 	 	
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In all cases the algae grown in Gorham's medium required higher
concentration levels of mercury, zinc, copper and cobalt to produce
algistatic conditions. The algistatic heavy metal concentration
levels were obtained by determining changes in chlorophyll ^content
of the test algae during a seven day incubation period and are considered
to be preliminary.
Full scale studies utilizing the "Algal Assay Procedure: Bottle
Test" are underway. The cultures are being spiked individually
with mercury, copper, and zinc. Growth will be analyzed by cell numbers
(electronic particle counter) and chlorophyll (fluorescence).
Algal photosynthesis and respiration studies will parallel the
"Bottle Test" assays to determine the effects of the heavy metals
upon these aspects of algal physiology.
Work Plan ZBP: Lake Restoration
Completion date of the tertiary treatment plant at Ely, Mn., was
extended to Septentoer 18, 1972, after consideration of the contractor's
request. This request was entirely reasonable and to the advantage
of EPA since authority to provide operating and back-up laboratory
positions are already long overdue. The completion date will have
to be further extended due to a strike of construction workers which
began on June 19.
Assistance on the treatment plant sludge disposal problem
was received from EPA-AWTRL and EPA Solid Waste Disposal, Cincinnati.
Inspection of disposal sites and meetings with State, County, City,
and U. S. Forest Service personnel were held. The most expedient
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approach for EPA from the standpoint of funds and time available will
be sanitary landfill disposal of dewatered combined tertiary and
digester sludges. The state has yet to give official approval.
Potential methods of land surface spreading will also be pursued
with the U. S. Forest Service as a possible future alternative.
Graver Company (process equipment supplier for tertiary plant)
sent a chemist to the Ely laboratory and with cooperation of the staff
of the Ely office, jar tests were carried out on municipal waste water
to evaluate means for tertiary plant operation. The results again
indicated 0.05 mg/1 phosphorus final effluent concentration but very
good process control will be necessary. Graver's report is pending,
however we expect that treatment will involve lime and a polymer in the
first contact unit followed by recarbonization to pH 9.2 and possibly
a coagulant in the second contact unit. Filtration is essential as
has been known.
Observations were made of the flow pattern (under ice conditions)
of Rhodamine WT dye placed in the secondary wastewater outfall on
March 27. Dye movement was first northwestward then eastward. The bulk
of the dye was detected in 7 to 15 foot depths. On May 1, dye was
detected in Shagawa River (lake outlet). No dye was detected in the
west end of the lake.
Ely staff found that the analytical and data evaluation workload
as currently proposed cannot be handled without additional staff as
requested for plant and laboratory operation. Therefore, pending
the reporting of these additional personnel, the schedule workload
was temporarily reduced.
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Total phosphorus in Shagawa Lake increased substantially through
the winter and reached a maximum in mid-April at Brissons Point (BP)
of 0.38 mg/1 and mid-May at east end deep hole (EEDH) of 0.12 mg/1.
Orthophosphate-P increased to a maximum of 0.06 mg/1 at BP on
April 5 and 0.08 mg/1 at EEDH on April 11.
Orthophosphate concentrations in the euphotic zone greatly
diminished after ice-out, presumably due to algal uptake.
Concentrations of orthophosphate have been less than 0.010 mg/1
phosphorus in the epilimnion since ice-out while total phosphorus
has ranged from 0.016 to 0.050 mg/1.
Areal concentrations (average of 3 stations) of chlorophyll a^
2
peaked at 155 mg/m about the first of June then declined to a
2	2
minimum of 98 mg/m the middle of June and increased to 144 mg/m
on July 6. Algae were stratified near the surface at the time of
peak production in May, but not so in late June with wind-mixing
being more frequent.
Algal counts on Shagawa River (lake outlet) at ice-out showed
green flagellates were dominate - mostly Chlaniydomonas and Euglena sp.
Predominate during the spring bloom were Asterionella formosa, Anabaena
circinalis, Me1osira, Chlamydomonas, and Synedra. On July 5, 6 the
phytoplankton population was predominately diatoms: Fragi1aria
crotonensis , Melosira, and Tabellaria. Also conspicuous were
Anabaena circinalis and Aphanizomenon flos-aquae.
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There was a clearly defined thermocline in Shagawa Lake by mid-May
with a 9°C temperature gradient between the 10 ft. and 20 ft.
depths by June 14. The stratification still existed on July 6
even though relatively high winds occurred during June. This is the first
time that stratification such as this has been observed in Shagawa
Lake. Anoxic conditions were observed below 25 ft. on June 13, 14 and
continued to exist on July 6.
Tributary nutrient loading for Shagawa Lake was determined for
the years 1967 thru 1971. Also determined was the contribution from
rainfall, Stinky Ditch and excess city water. Graphs were plotted
for 1imnological, climatological , treatment plant and tributary trends.
Work Plan ZCH: New Methods for the Removal and/or Inactivation
of Nutrients in Lakes.
The Clines Pond sodium aluminate phosphorus inactivation
experiment continues to be monitored every two weeks. Although
marked improvement of the pond was observed throughout the 1971
growing season, this year the pond appears to have reverted to
its pretreatment state. Heavy Anabaena blooms have occurred
during the spring, with average Secchi disc values of about
50 cm. Dissolved oxygen in the bottom waters is low. It is not
possible to determine whether inactivated phosphorus has been
released, but it is known that the drainage basin of the pond
has received agricultural fertilizer, and that commercial fish
food was applied to the pond by the owner. Therefore, it is
possible that the increased algal production this year is the
result of these recently added nutrients.
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Work Plan ZCE: Sediment - Water Plant Nutrient and Toxic Element
Interchange Processes and Control
Leaching experiments on Shagawa Lake sediments from four
locations in the lake, initiated last quarter, were completed.
Phosphorus release rates were much greater (1) under anaerobic
conditions, and (2) from non-mixed sediment-water systems. In a
non-mixed carboy and laminar flow systems phosphorus release was
greater than in a. stirred carboy system.
Work Plan ZCJ: A Comparative Study of Eutrophication in Two Mountain .
Lakes.
The Diamond Lake study was resumed in the last week of May,
two weeks following ice-out. Nutrient levels were about the same
as those encountered on the initial sampling trip last year and a
substantial diatom population was found. The second trip was on
26-28 June. At that time no algal bloom was in evidence, and Secchi
disc values of 25 feet were obtained. Growth of submerged macrophytes
was under way. In summary, so far we have observed no change in lake
conditions from those of last year.
Other Activities
Mr. Maloney visited with the Special Projects staff at Headquarters
to discuss the lake survey program, including tributary sampling and
aerial photography. He also met with the Deputy Assistant Secretary of
Defense for Reserve Affairs and his staff to discuss tributary sampling
by the National Guard and Reserve Units. Following he met with WERL
personnel at Griffis Air Force Base, Rome, New York to discuss the lake
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sampling and with personnel at the Rome Air Development Center to
discuss the use and interpretation of aerial photography.
Ken Malueg attended a meeting in Vancouver, Washington regarding
future restoration plans for Vancouver Lake, Washington. Bob Randall
(Ely Staff Chemist) visited Corvallis to work and discuss with CLS
analytical methodology and instrumentation. Interviews at Ely were
held with reporters from KBAL-TV, Channel 3, Duluth, by the editor
of Wilderness News (Minneapolis) and by the Minneapolis-Tribune newspaper
regarding the Shagawa Lake project.
Mr. Maloney attended a modeling workshop at RTP, North Carolina
and discussed mathematical modeling in the National Eutrophication
Research Program.
Mr. Maloney attended the 6th International Conference on Water
Pollution at Jerusalem, Israel and presented a paper entitled "Use
of Algal Assays in Studying Euthrophication Problems."
Mr. Maloney, accompanied by Mr. Herb Quinn of the office of
International Affairs met in Tunis with the staff of the Institute
National Scientifique Et Technique Oceanographic Et Peche (INSTOP)
to develop a joint research project (under the Special Foreign
Currency Program) between EPA and the Government of Tunisia.
Visitors included Dr. Arlo Fast (Union Carbide Corp) who
discussed aeration as a lake restoration technique and Dr. Eschenroeder
(General Research Corp) who discussed future efforts he would like to
pursue in developing predictive lake models.
William Sanville and Spencer Peterson successfully completed a
course in SCUBA.
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Jevil's, Woahink, Marion, and Paulina Lakes were visited in search
for suitable sites on which to carry out studies of nutrient flux to
lakes from septic tanks. A mobile phosphorus-removal pilot
treatment plant was obtained from Battelle-Northwest for use in lake
restoration studies.
The following manuscripts were reviewed by NERP personnel:
1.	Lake Aeration, by Arlo Fast of Union Carbide Corporation.
2.	Horseshoe Lake Nutrient Inactivation Study, Dy various
Wisconsin investigators.
3.	OECD's "Report of the Planning Group on Measurements
and Monitoring for Eutrophication Studies."
Grant and Contract Research
Tne following research grant and contract proposals were reviewed.
1.	"Blue-Green Algal Bloom Induction"
2.	"A Study of Eutrophication Potentials of Selected Surface
Waters of the Northeast" (Proposed)
3.	"Primary Productivity Limitation in Bear Lake, Utah"
4.	"The Role of Sludge Worms in Nutrient Transport"
5.	"De-eutrophication via Aeration in Cylindrical Macromicrocosms :
Biology, Dynamics, Effects and Their Implications Toward Large Ecosystems.
Part I. Equipment and On-gotng Research, and Part II. Cylinders and
Set-up."
6.	"Stratigraphic Techniques for Determining Baselines of Lake
Pollution."
7.	"tutrophic Lake Reclamation by Physical and Chemical
Manipulations."
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8.	"Studies on the Nitrogen Metabolism of the Blue-Green Algae."
9.	"Aqueous Environmental Chemistry of Nitrogen and Phosphorus in
Lake Mendota."
10.	"Application of Algal Assays to Lake Renewal Projects,"
11.	"Relationship of Algal Extracellular Metabolites to Bloom
Sequence in Fresh Water."
12.	"Systems Studies of Water and Nutrient Transport.1'
13.	"Routine Chemical Methods for Quantifying the Phosphorus Release
Potential of Sediments."
14.	"Relationship Between Chemical Control of Algae or Aquatic
Weeds and Eutrophication."
15.	"Nutrient Inputs to Natural Waters."
16.	"Eutrophication Information Program."
17.	"Synthetic Agricultural Fertilizers." (Proposal)
18.	"National Lake Inventory."
19.	"Nitrogen Transformation in Lake Sediments."
20.	"Eutrophication of Surface Waters - Lake Tahoe (Indian Creek
Reservoi r)."
21.	"Continuous Steady-State Algal Assay Methods for Eutrophication
Studies."
22.	"The Significance of Bacteria in Eutrophi cation of Lakes."
Reports, Papers, and Presentations
Tilstra, J. R., K. W. Malueg and W. C. Larson. "Removal of
Phosphorus and Nitrogen from Wastewater Effluents by Induced Soil
Percolation. J. WPCF. Vol. 44, No. 5, May 1972.
Maloney, T. E., W. E. Miller and N. Blind. "Use of Algal Assays in
Studying Eutrophication Problems." Presented before 6th International
Conference on Water Pollution Research, Jerusalem, June 12, 1972.
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Plans for First Quarter FY 73
Physiological Control
1.	Continue the study of the trace metal requirements of the
three AAP test alga.
2.	Continue the evaluation of new test species for algal assays.
3.	Continue development of an algal assay procedure for marine
and estuarine waters.
4.	A meeting of University and industrial researchers involved
in the evaluation of the algal assay procedure will meet
at this laboratory on August 16-17.
Lake Restoration
1.	Complete nutrient budget of Shagawa Lake for the years 1967
through 1971.
2.	Obtain authorization and hire critically needed personnel
for Shagawa Lake project.
3.	Continue limnological investigation of Shagawa Lake.
4.	Survey and construct bathymetric map of Shagawa Lake.
Technology Development
1.	Continue Diamond Lake study. C-14 productivity will be
measured every two weeks, and a complete survey will be carried out
once each month. A SCUBA survey of weed bed development will be run
the last week in July.
2.	The annual survey of Waldo Lake will take place in August.
3.	Laboratory experiments on nutrient inactivation and sediment-
water interchange will be initiated in July.
4.	Monthly monitoring of Suttle Lake will continue.
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5.	Reports on the Cline's Pond nutrient inactivation experiment;
ground water studies at Waldo Lake; the 1971 Diamond Lake studies; the
Lake Sal lie weed harvesting study; and the Shagawa Lake sediment-
water Pand N interchange experiments should be in completed manuscript
form by the end of the quarter.
6.	The contract with CHgM for an A&E study on experimental pond
design and construction is being negotiated and progress on the
study during the quarter is anticipated.
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NATIONAL EUTROPHICATION SURVEY PROGRAM
Status of Project and Significant Accomplishments
The National Eutrophication Survey Program (NESP) activities
at PNWL accelerated this quarter as the NESP staff began to come on
board, beginning with the Data Coordinator and the Secretary on
May 15. Six junior staff members were added on May 28, and
staffing was essentially completed by June 26 when the Program
Element Direcior and most of the remaining staff reported for duty.
Because of the late staffing of the Survey Program, necessitated
by the limi.ed FY72 budget, continued reliance was placed on
The National Eutrophication Research Program (NERP) and Consolidated
Laboratory Services (CLS) personnel of PNWL, as well as other
NERC-Corvallis personnel, for program assistance.
Lake sampling was begun in May by personnel of the Western
Environmental Research Laboratory, Las Vegas (WERL), with assistance
provided by CLS personnel of PNWL in field sampling analytical
procedures, quality control, and instrumentation. NERC-Corvallis
provided the assistance of limnologists from associate laboratories
for the field effort. On-site inspections of WERL survey procedures
and assistance in analytical techniques were provided by senior staff
members of NERP and CLS.
One of the primary efforts of the NESP staff during the quarter
was directed to assist Headquarters Special Projects Staff in the
selection of 233 study lakes and impoundments in 10 states, with the
concurrence and assistance of Region and State personnel.
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The major effort of the staff was in the selection of 192
tributary sampling sites associated with 28 study lakes and im-
poundments in the New England states, exclusive of Maine. This
effort involved confirmation of selected sites with Region and
State personnel, identification and location of point waste
sources, preparation of maps and site descriptions, and forwarding
of the maps and site descriptions to the Special Projects Staff
for reproduction and distribution to the various National Guard
units that will be involved in tributary sampling. As the quarter
closed, similar effort was being made in tributary sampling site
selection for 205 water bodies in the states of Maine, New York,
Michigan, Wisconsin, and Minnesota.
Staff attention was also given to the problems of obtaining
adequate data on the effluent nutrient levels of the many, frequently
small, wastewater treatment plants situated on tributaries to the
study lakes and impoundments as well as obtaining satisfactory flow
data for tributaries not presently gaged by the U.S. Geological
Survey or by state authorities.
Plans for First Quarter, FY73
The first quarter of FY73 will be devoted to (1) selection of
tributary sampling sites around study lakes in Michigan, Wisconsin,
Minnesota, Maine and New York, (2) training National Guard units and
initiating tributary sampling in the ten states studied in FY72, and
(3) selecting study lakes and tributary sites in Region 4 states in
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which sampling will begin in January 1973. Instructing National
Guard units in tributary sampling procedures will require considerable
assistance from EPA personnel from the National Marine Water Quality
Laboratory in West Kingston, Rhode Island, and other Associate Lab-
oratories in NERC-Corvallis in addition to the instructors from
NESP, and other PNWL personnel.
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NATIONAL WASTE TREATMENT RESEARCH PROGRAM
FOOD WASTES RESEARCH
Status of Projects and Significant Accomplishments
Work Plan AZQ: Technical Consultation and Data Dissemination
The effluent committee of the U. S. Brewers Association met witn
EPA's headquarters staff and Dennis Taylor to discuss future government
effluent restrictions.
On two occasions, Kenneth Dostal and Harold Thompson met with
Mike LaGraff of the headquarter's Effluent Guidelines and Standards
Development Program and representatives of the canned and frozen fruit
and vegetable processors. These meetings were held to establish procedures
that will be utilized to develop a broad data base for determining standard
raw waste loads and current treatment levels for the fruit and vegetable
processing industry.
A meeting between headquarter's enforcement and Refuse Act Permit
Program (RAPP) personnel and representatives of the canned and frozen fruit
and vegetable processors was attended by Kenneth Dostal and Harold Thompson.
RAPP Schedule A&B effluent guidelines for the fruit and vegetable
processing industry were the subject of this meeting.
On May 24th a paper titled "New Fruit and Vegetable Processing Waste
Management Systems" was presented by Harold Thompson at the 72nd National
Meeting of the American Institute of Cnemical Engineers.
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All papers, with one exception, presented at the Third i^tional
Symposium on Food Processing Wastes have been received. The Proceedings
of this symposium will be submitted for reproduction in the near future.
Work Plan FGF: Grant Monitoring
Visits were made to the following grant sites during the fourth
quarter of Fiscal Year 1972:
1.	Crowley Food, Inc., La Fargeville, NY - 12060 DXF
2.	Dairy Research and Development Corp., Vernon, NY - 12060 DEQ
3.	Widmer's Wine Cellars, Naples, NY - 12060 EVZ
4.	Amber Laboratories Division, Juneau, WI - 12060 HRR
5.	Western Potato Service, Inc., Grand Forks, rtD - 12060 EIG
6.	City of Grand Forks, ND - 12130 DJB
Grant proposals and preproposals on tne following subjects were
received during the past quarter:
1.	Sludge disposal at the main plant of a meat packing plant.
2.	Membrane processes in the treatment of potato protein waste
water—reverse osmosis and ultrafiltration.
3.	Azeotropic processing.
4.	A wastewater treatment plant for a soybean products processing
pi ant.
5.	Treatment of concentrated wastewater from the citrus processing
i ndustry.
6.	Pollution aspects of catfish production—review and projections.
7.	Effluent guidelines for specialty food industry.
8.	Cat fish -Fanning wastes.
The current status of grants monitored under the Food Wastes Research
Program is described on the following page:
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1.	12060 EUZ - "Winery Was tewater—Characteri zati on and Treatment.
Post construction studies are continuing.
2.	12060 EKQ - "Kent Cheese Company—Waste Treatment Facility."
First draft of final report has been reviewed and returned to grantee for
revision.
3.	12060 FLL - "Activated Sludge--Bio Disc Treatment of Distilling
Wastes." Data collection and evaluation on the operational parameters of
the waste treatment facility was continued throughout the past quarter.
4.	12060 EHS - "Cannery Waste Treatment by Lagoons and Oxidation
Ditch." The final report is being retyped before submittal for
reproduction.
5.	12060 EHV - "Aerobic Secondary Treatment of Potato Processing
Waste with Mechanical Aeration." Grant has been audited but not official
compl eted.
6.	12060 DSI - "State-of-the-Art, Sugar Beet Processing Waste
Treatment." The final report has been reproduced and initial
distribution completed.
7.	12060 ECF - "Water Pollution Abatement in the United States
Seafood Industry—Stete-of-the-Art." Grant awaiting final audit.
8.	11060 EZR - "Complete Aerobic Treatment of Combined Domestic
and Industrial Wastes with Mechanical Aeration." Grant has been audited
but not officially closed.
9.	12060 EIG - "Full-Scale Demonstration and Evaluation of Potato
Dry and Wet Caustic Peeling Processes." First draft of final report has
been reviewed and returned to grantee for revision.
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10.	WP-01486-01 - "Status and Research Needs for Potato Waste
Waters." This grant has been audited but not officially closed.
11.	12060 FAD - "Aerobic Treatment of Fruit Processing Wastes."
Grant has been audited but not officially closed.
12.	WPD 93-04-68 - "Anaerobic-Aerobic Sugar Beet Waste Treatment."
Final report has been submitted for reproduction and distribution.
13.	12060 DXL - "Reduction of Salt Content of Food Processing
Liquid Waste Effluent." Grant has been audited but not officially closed.
14.	12060 EDK - "Production and Disposal Practices for Liquid Wastes
from Canning and Freezing Fruits and Vegetables." Final report has been
reproduced and distributed.
15.	12060 EHT - "Use of Fungi Imperfecti in Waste Control." Grant
has been audited but not officially closed.
16.	12060 EDZ - "Pilot Plant Installation for Use of Fungi Imperfecti
on Vegetable Wastes." Grant has been audited but not officially closed.
1 7. 12060 FAK - "Concentration of Sugar Beet Wastes for Economic
Treatment with Biological Systems." Grant has been audited but not
officially closed.
18.	12060 FQF - "Dry Caustic Peeling of Tree Fruit to Reduce Liquid
Waste Volume and Strength." Final audit has been conducted but grant has
not been officially closed.
19.	11060 FJQ - "Pollution Abatement and By-Product Recovery in
Shellfish and Fisheries Processing - Phase 1." Final audit requested.
20.	12060 EGU - "State-of-the-Art of Dairy Plant Wastes and Waste
Treatment Systems." Final report has been reproduced and distributed.
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21.	12060 FDR - "Disposal of Rum distillery Wastes." Second draft
on final report has been reviewed and returned to grantee for revision.
22.	12060 ESY - "Improvement of Treatment of Food Industry Waste."
Second draft of final report under review.
23.	12060 EZP - "Cannery Waste Treatment by the Kehr Activated
Sludge Process." Grant has been audited but not officially closed.
24.	WPRD 151-01-68 - "Integrated Treatment of Liquid Wastes from
Food Canning Operations." Final audit has been completed but not
officially closed.
25.	12060 EHU - "Reconditioning and Reuse of Food Processing Brines
Grant has been audited but not officially closed.
26.	12060 DQV - "Removal and Recovery of Fatty Materials from
Edible Fat and Oil Refinery tffluents." First draft of final report is
being revised.
27.	12060 DEQ - "Elimination of Pollution by and Utilization of
Protein Concentrates (Dried Whey) from Milk Residues of Cheese Making."
Full scale waste treatment system has been constructed and data
collection initiated.
28.	12060 DPE - "Treatment of Wastes from the Wet Milling Industry.
Final report is being prepared.
29.	12060 DSB - "Demonstration of a Full-Scale Waste Treatment
System for a Cannery." Final report has been published and distributed.
30.	12060 EAE - "Evaluation of Controlled Temperature and Forced
Aeration in Trickling Filter Treatment of Food Canning Waste Waters."
Final report has been reproduced and distributed. Grant has been audited
but not officially closed.

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31.	12060 DXF - "Development and Demonstration of an Ultrafiltration
Plant for the Abatement of Pollution from Cottage Cheese Whey." Final
report on Phase 1 completed and full-scale construction has been completed
and Phase II data collection initiated.
32.	12060 FJK - "Acid Emulsion Breaking - Activated Sludge for
Bakery Waste." Post-construction studies underway.
33.	12060 ESC - "Separation, Dewatering and Disposal of Sugar Beet
Transport Water Solids." First draft of final report on Phase I has
been received.
34.	12060 FUR - "Membrane Separation of Soybean Whey for Product
Recovery and Waste Treatment." Data collection completed. Evaluation
and final report preparation underway.
35.	12060 FTC - "State-of-the-Art Study of Water Pollution Control
from the Beverage Industry." The final report still under revision.
36.	11060 DLF - "Tertiary Treatment of Combined Domestic-Industrial
Wastes." The final report still under preparation.
37.	11060 DJB - "Controlled Treatment of Combined Potato Processing
Municipal Wastes by Anaerobic Fermentation, Aerobic Stabilization Process."
Post-construction studies underway.
38.	11060 DUJ - "Dynamic Process Development for Biological
Treatment of Whey Bearing Wastes." Final report submitted for reproduction.
39.	11060 ENF - "Vermont Cheese Industry Pollution Abatement."
Original program scope is being revised.
40.	12060 PAV - "Low Water Volume Enzyme Deactivation of Vegetables
Before Preservation." Final report is being prepared by the grantee.
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41.	12060 HFY - "Dry Caustic Peeling of Clingstone Peaches on a
Commercial Scale." First draft of final report is being revised.
42.	12060 FRW - "Water and Waste Management in Sweet Potato
Processing." Grantee's funds have been temporarily suspended until their
processing plant, which was destroyed by fire, is rebuilt.
43.	12060 HCW - "Submerged Combustion Evaporation System for
Concentration of Brewery Spent Grain Liquors," Data collection still
underway.
44.	12060 HPC - "Pilot Scale Treatment of Wine Stillage." Additional
anaerobic units as well as the aerobic pilot plants were placed onstream.
45.	12060 HUQ (801007) - "Seafoods Processing Wastewater Character-
ization." Waste characterization studies underway.
46.	12060 HRR (800747) - "Acid Whey Fermentation Demonstration Pilot
Plant." Data is currently being collected.
Four grants were awarded during the past three months. These grants
are:
47.	800904 - "Shrimp Canning Waste Treatment Study," American
Shrimp Canners Association. The general objectives of this grant are to
characterize and treat (pilot scale) waste water from the processing
of shrimp.
48.	800250 - "Continuous Hot Air Blanching of Vegetables," National
Canners Association. The primary objective of this grant is to demonstrate
the technical and economical feasibility of hot-air blanching of vegetables.
49.	800935 - ,rRum Distillery Waste Treatment by Anaerobic Digestion,"
Bacardi Corporation. Pilot scale anaerobic treatment of rum distillery
wastes will be evaluated by this grant.
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50. 801221 - "Ecostatic Cane Processing System--Pilot Phase,"
County of Hawaii. The primary objective of this grant is to develop
an economical system which will eliminate liquid-borne wastes from
sugar cane processing.
Plans for First Quarter - Fiscal Year 1973
1.	Continue grant monitoring.
2.	Continue work on industrial effluent limitations.
PAPER AND FOREST INDUSTRIES RESEARCH
Status of Projects and Significant Accomplishments
Work Plan GJG: Technical Consultation and Data Dissemination
Information and assistance by the section was continued
to the regional offices and other government agencies. Requests
from San Francisco, Boston, and Seattle Regional Offices for information
were handled. Continued effort was made on the study of wood
preservation wastes. Mr. Scott met with personnel from the Office
of Saline Water in Denver, Colorado. A visit and tour of the
Weyerhaeuser Company's operations at Springfield, Oregon, was
made by the whole PFI section. Mr. Scott attended the annual
Environmental Meeting of TAPPI in Houston, Texas, and will be
serving on next year's meeting corranittee. During an OSU School
of Forestry Young Growth Management short course, Dr. Groman presented
two papers. He also visited the Pressure Treating Lumber Company
in Sheridan, Oregon, along with Mr. Rossman, a new employee in
the section. The seminar on source sampling by the Air Pollution
Control Associates PNWI Section in Portland was attended by
23

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Mr. Rossman. Dr. Gallup from the Industrial Pollution Control
Branch, Washington, DC, visited the lab and was shown projects
in Camas, Washington, and Lebanon, Oregon. A meeting on the Miami
Conservancy District's automated regional treatment facilities
was attended by Dr. Willard in Chicago. He also presented a paper
on air and steam stripping of pulp mill wastes at Purdue University's
Industrial Waste Conference in Lafayette, Indiana. A paper on
a rotating biological treatment system for hardboard wastes was
co-authored by Willard, Scott, and Eckerle for the Forest Product
Research Association's annual meeting in Dallas, Texas. Dr. Knittel
attended a meeting on K1 ebsiella at the Denver National Field
Investigation Office. Present progress of identifying the significance
of this organism in pulp and paper mill effluents was presented.
Work Plan FCK: Paper Mill Influence on Bacterial Quality
Work has continued on the cultural comparison of Klebsiella pneumoniae
isolated from pulp mill effluents and those K_. pneumoni ae obtained from
American type culture collection. This comparison has shown that from a
substrate utilization standpoint there is no difference between these
cultures.
Preliminary results of nucleic acid analysis on DNA isolated from
these cultures also points out that there is, so far, no demonstrable
di fference.
24

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Work Plan GJH: Suspended Solids Removal by Mechanical Means
The program to evaluate microscreening of unbleached sulfite suspended
solids at Crown Zellerbach's Lebanon, Oregon, mill continued throughout
the quarter. The first two phases of the project, microscreening
raw wastewater and aerated lagoon, receiving primary settled waste,
effluent have been completed using 10, 42, and 100 micron screens.
Suspended solids, settleable matter (by weight and volume), BOD,
and soluble BOD were determined. The aeration tank influent
has been shifted from primary effluent to raw wastewater. The
presence of large chips in the raw wastewater has caused some
flow stoppage and delays. The use of larger lines and a coarse
sidehill screen appears to be eliminating the problems at this
ti me.
Work Plan ZEB: Biological Systems for High Efficiency Treatment of
Hardboard Wastewater
The investigation of treating hardboard wastewaters using
a rotating disc device has been carried out at a plant in Corvallis,
Oregon. With the addition of mineral nutrients, a removal of
90% BOD and 70% dissolved organic carbon was attained at a loading
of 0.52 gal/sq ft/day or 0.20 mg BOD applied/mg S.S. The final
effluent contained a flocculent biomass that settled very rapidly.
Current results were presented in a paper given at the Annual
Meeting of Forest Products Research Association in Dallas, Texas,
on June 21.
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Work Plan FCD: Grant and Contract Monitoring
Grant applications and research proposals reviewed during
the quarter related to the following:
Pyrolysis of Industrial Waste for Oil and Activated
Carbon Production
Industrial Waste Study of Wood Preserving Wastes
Water Reuse in a Paper Reprocessing Plant
Two projects were prepared for funding through the NERC-
Corvallis Center: "A Test Method for Volatile Component Stripping
of Wastewater in Cooling Towers" and "Pulping Chemicals Recovery
and External Waste Treatment Comparison."
Four new projects were assigned project officers from the
section. These new grants were:
S 800520 - R. H. Scott - "Closed Process Water Loop in
NSSC Pulp Production" - Green Bay Packaging,
Green Bay, WI.
S 800915 - H. Kirk Willard - "Fate of Nitrogen Fertilizers
in Forest Soil: Conversions, Movement, and
Losses" - Crown Zellerbach, Camas, WA
S 801206 - J. S. Ruppersberger - "Water Reuse in Paper
Processing Plant" - Big Chief Roofing, Ardmore, OK
S 801207 - R. H. Scott - "Treatment of Sulfite Evaporator
Condensates for Recovery of Volatile Components
Institute of Paper Chemistry, Appleton, WI
26

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Other active grants showed the following progress during
the quarter:
1.	12100 EZV - Reactivation and final data analysis. Additional
work is being accomplished to complete this project as determined
in the final report draft review.
2.	12100 EBG - The final report draft was submitted and
reviewed on this log rafting project. A meeting was held on the
required changes and agreements reached on included material and
recommendations.
3.	12040 EEL - "Reverse Osmosis is Concentration of Dilute
Pulp and Paper Effluents." The final report was printed and distributed.
4.	12040 FUB - "Recycle of Papermill Wastewater for Application
of Reverse Osmosis." This final report was printed.
6.	12130 GER - "Optimization of Combined Industrial/Municipal
Waste Treatment through Automation and Reuse." Engineering design,
plans, and specifications have been completed.
7.	12040 GQD - "Coliform Growth and Control in Aerated
Stabilization Basins." Studies on mill scale chlorination have
begun with the completion of pilot plant chlorination studies.
8.	12040 DBD - "Color and Mineral Removal from Kraft Bleach
Wastes." The project has been extended to allow additional sampling
of trials on a new resin.
During the quarter, considerable effort was exerted on planning
the new Research Objective Achievement Plans for the next six years.
Detailed tasks were included which should develop the necessary
technology to operate closed-loop pulp and paper operations.
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Plans for First Quarter, FY 1973
1.	Continue R&D grant monitoring and review for approved ROAP
areas.
2.	Provide consulting services upon request to regional offices,
headquarters and the states.
3.	Continue work on the investigation of screen size effect on
suspended solids removal from sulfite waste and work to determine the
potential health hazard that Klebsiella may present.
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NATIONAL THERMAL POLLUTION RESEARCH PROGRAM
Status of Projects and Significant Accomplishments
Again, as in last quarter, a large percentage of staff effort
has been devoted to review of Environmental Impact Statements on
nuclear power plants, preparation of technical testimony for the
Houston Lighting and Power suit, and consultation to Headquarters
and Regional Offices on discharge permit applications. Preparation
of Research Objective Achievement Plans (ROAPs) took considerable
time.
ROAP 10 BAL: Beneficial and/or Multiple Use of Industrial Water
Involvi ng Coolinq
Guy Nelson began literature review of complete recycle of liquid
streams in power plants. A visit to Centralia Power Plant where
cooling tower blowdown is used as makeup to ash handling and coal
washing facilities is planned.
ROAP 16 ACQ: Wet Closed-Cycle Cooling Systems--Sa1t and Fresh Water
RFP on blowdown control techniques submitted and approved for
publication. Advertising will start on August 1, 1972, with contract
awarded by October 1 , 1972. Literature review for inhouse portion
of report has already begun.
ROAP 21 AJH: Predictive Model for Aquatic Thermal Pollution
The work on the towing channel for simulating thermal plumes
has been going along with measurable success. Discharge tanks and
manifolds as well as instrumentation problems have been worked out.
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Jim Chasse's efforts since he recently joined HTPRP staff have been
exclusively directed to helping Dr. Winiarski on thermal plume
simulation.
Thermal Power Plant Discharge Guidelines
Industrial Waste Studies Program. Work is continuing on
the guidelines in cooperation with Edison Electric Institute. A
meeting was held in Headquarters, April 25, 1972, and Alden
Christianson of our staff attended. This meeting was for the purpose
of devising a questionnaire that EEI has indicated they would
distribute to member power plants to obtain information on
constituent pollutants, treatability, treatment efficiency and
costs. All of this is required for input to the establishment of
effluent guidelines. Follow-up will include contacting other power
groups for possible distribution of questionnaires; line up EPA
consultants and contact design-engineering firms to (1) generate
additional data, and/or (2) review and validate data gathered from
others.
Technical Assistance
The following environmental Impact Statements were reviewed.
1.
Edwi n I. Hatch, Uni ts
1 and 2
2.
Quad Cities, Addendum,
Units 1 & 2
3.
J. M. Stuart Plant

4.
Shoreham

5.
Monti eel lo

6.
Aguirre, No. 1

7.
Wm. H. Zirrener Station

8.
Fort Calhoun, Unit 1

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9.
Browns Ferry--Additional Comments
10.
Joseph M. Farley, Units 1 & 2
11 .
Maine Yankee Atomic Power Plant
12.
Oconee Nuclear Station, Units 1 & 2
13.
Fort St. Vrain
14.
Watts Bar Nuclear Plant
15.
Indian Point, Unit 2
16.
Vermont Yankee Nuclear Power Station
Further
consultation to Headquarters included:
1.	May 18, 1972, Dr. Tichenor traveled to Washington
for a committee meeting on the development of guidelines for
implementing EPA's policy on thermal discharges. This committee
is responsible for preparing a report presenting technical guidelines
to EPA Regional personnel responsible for evaluating thermal
discharges on a case-by-case basis.
2.	Industrial Waste Studies Program. See section on
Thermal Power Plant Discharge Guidelines.
3.	June 7 and 8, Drs. Tichenor and Shirazi participated in
the preparation of a handbook, "Selected Techniques for Case-by-
Case Evaluation of Thermal Discharges." This work was conducted
at the request of the Office of Technical Analysis in the Office of
Enforcement and General Counsel (see 1 above).
4.	Various proposals received and reviewed.
5.	Polish Special Foreign Currency Research Program
reviewed and Mr. Rainwater has been appointed Project Officer. See
trip to Poland under plans for first quarter, FY-73.
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Consultation to Regions:
1.	At the request of Gary Rochelle, Control Systems
Division, NERC, RTP, NTPRP solicited comments from NWQL, NMWQL,
AWTL-Cincinnati, and various persons in Headquarters on Impact of
Air Pollution Control Systems on Water Quality. A compilation of
this information was forwarded to RTP April 1 3, 1972.
2.	Critique on desk-top study of potential for heat
recovery from thermal discharges applied to advanced waste treatment
completed for AWTL, Cincinnati.
3.	Dr. Shirazi traveled to Dallas, June 21-23, to
finalize the report on the use of the Galveston Bay Hydraulic Model
for the Houston Lighting and Power case.
4.	Discussions with Region VII personnel on evaluation of
thermal discharges.
5.	Technical assistance to Region V in the form of an
evaluation of temperature prediction models as applied to the Ohio
River in the vicinity of the J. M. Stuart plant.
6.	Water Modeling Workshop, June 6 & 7 at RTP, at the
request of Dr. Wiser. Mr. Rainwater presented a brief report on
modeling activities within NTPRP.
7.	Mr. Rainwater met with Regional Office personnel,
Region IX, and representatives and consultants for Hawaii Electric
Company re discharge permits for Hawaii Electric.
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Grant and Contract Research
RQAP 21 AJH: Predictive Model for Aquatic Thermal Pollution
1.	Environmental Systems Laboratory, Inc., Grant No. 16130 GSD,
"Study for the Stochastic Calculation in Water Equilibrium
Temperature," Is continuing.
2.	Oregon State University, 16130 FOK, "Controlling
Pollution in Small Streams." Final report received.
3.	Cornell University, 16130 DIP, "Heat and Water Vapor
Exchange between Water Surface and Atmosphere." Final report in
preparation.
4.	Oregon State University, 16130 DGM, "Thermal Plume
Dispersion," is continuing.
5.	MIT, 16130 DJU, revised surface jet model user's manual--
in preparation.
RQAP 16 ACQ: Wet Closed-Cycle Cooling S.ystems--Sal t and Fresh Water
1.	Hittman Associates, 16130 HKK, "Thermal Pollution Control
Nomographs," is continuing.
2.	Washington State University, "Analysis of Engineering
Alternatives for Environmental Protection from Thermal Discharges,"
is continuing--no-cost extension granted.
3.	Environmental Systems Corporation, 16130 GNK, "Explicit
Calibration of the PILLS System," is ongoing.
4.	The Center for the Environment and Man, Contract 14-12-837.
Received final report.
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ROAP 10 BAL; Beneficial and/or Multiple Use of Industrial Water
Involvi nq Cooli nq
1. Mr. Christianson and Mr. Herman Miller, EWEB, Grant #16130
EIK, put together Intended Approach Toward Work Funding, and Completion
of the EWEB demonstration project.
Reports, Papers, Meetings
1.	Paper entitled, "Dry Cooling Towers for Steam Electric
Power Plants in Arid Regions," will be published this year in Journal
of International Water Pollution Research. Final draft has been
reviewed and sent to publisher.
2.	Workbook on Thermal Plume Prediction, Vol. 1: Submerger
Discharges, Dy Mostafa A. Shirazi and Lorin R. Davis, has been
submitted for publication in the Water Pollution Control Research
Series. This report will be used as text for the Workshop on Plume
Prediction, to be held at PNWL July 11-14, 1972. Fifty preliminary
copies were printed for this purpose.
3.	Dr. Shirazi attended a course on Estuarine Modeling at OSU,
June 12-16, 1972. Dr. Leendertse of Rand Corporation lectured on
recent advances on estuarine modeling for tidal dynamics, as well
as for water quality.
4.	Alden Christianson attended the American Nuclear Society,
Special Session on Geothermal Energy, Las Vegas, Nevada, June 19-20,
1972, to keep abreast of current geothermal development and related
pollution potential .
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Visits , Vi s i_tors
1.	On May 3, i'ir. James Mctlroy, meteorologist at KTP,
visited NTPRP to discuss mutual research interest.
2.	Bill Schofield, RTP, visited NTPRP may 5 to discuss EROS,
ROAPs, Work Plans and contract specifications for the salt water
cooling demonstration at Turkey Point.
3.	Mr. Larry Ollinger of EPA, Ft. Lauderdale, Florida, met with
Dr. Shirazi on April 26-27 to discuss plume analysis for an assignment
he had for the Athens Lab.
4.	Dr. Amiran Roffman, Westinghouse Electric Corporation,
visited May 16 re state-of-the-art on cooling systems.
5.	Dr. John Drayley, Argonne National Lab, visited NTPRP
May 16 for discussion of plume analysis.
6.	Dr. Tichenor visited Region VII on June 9 for demonstration
of Cherne Thermal Rotor Cooling System (Cherne Industrial, Inc.).
7.	Dr. Shirazi visited Louisiana State University on June 20
to confer with LSU staff on modeling.
Plans for First Quarter, FY-73
1.	Dr. Tichenor will present a paper, "Evaluating Thermal
Pollution Control Alternative," at the Institute of River Mechanics,
Colorado State University, August 2, 1972.
2.	Mr. Rainwater will present a paper, "Federal Standards and
Thermal Considerations," at the University of Wisconsin's Cooling
Towers Institute, August 9, 1972.
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3.	In September, Mr. Rainwater will travel to Warsaw, Poland to
participate in Special Foreign Currency Workshop for U. S. Project
Officers and Polish principal investigators of new projects; and
to make site visits where work will be performed. Stops are planned
in England, Germany and Sweden to learn more of their work in
Thermal Pollution Control especially with respect to dry cooling
systems.
4.	Continue inhouse portion of state-of-the-art report on
blowdown control.
5.	Review Environmental Impact Statements.
6.	Industrial Waste Survey Guidelines.
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NATIONAL COASTAL POLLUTION RESEARCH PROGRAM
Status of Projects and Significant Accomplishments
Work Plan DBH: Estuarine and Ocean Outfalls
The Interagency Agreement between NCPRP and the Environmental
Prediction Research Facility, U. S. Navy, Monterey, California,
requested last quarter was approved. The agreement concerns
development and testing of a numerical model of pollution
dispersion in the New York Bight area.
Chief, Physical Oceanography Branch served as a coordinator
with the Department of Oceanography and Civil Engineering,
Oregon State University, on a short course held in June. About
20 students attended the course on digital computer techniques
for tidal modeling of well-mixed estuaries and shallow bays.
Equipment for the coastal flushing and circulation experiment
was received, tested, and calibrated. Deployment of the instruments
is scheduled for mid-July. The instruments will remain on the
ocean bottom for about 30 days .
Work on drift flow models continued. As part of the report,
a model developed for use off Florida was examined. Several
serious errors in the report discussing the application of the
model were discovered and brought to the attention of the
originators of the report through the Southeast Region.
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EPA Region X Working Paper #80 entitled "User's Guide and
Documentation for Outfall Plume Model" was revised to improve
the model's sensitivity for low Froude Number Outfalls with small
angles of inclination. Holders of this working paper who have
not received an errata covering this change are requested to
contact this office.
Work Plan ZAH: PCB, Biocides, and Other Persistent Organic
Compound Distributions and Fates in Coastal Waters
Dr. Blazevich attended an interagency meeting of PCBs in the
environment in Duluth, Minnesota on May 22-23, 1972. Presentations
of present efforts were given by fourteen laboratories followed by
a work session concerning recent advances in analytical techniques
used to identify and quantify PCBs.
Dr. Blazevich attended EPA's gas chromatograph/mass
spectrometer club meeting at Dallas, Texas on June 8, 1972. He also
travelled to Berkeley, California on June 14, 1972, to give
technical assistance to Mr. Rittall who was conferring with a
grantee.
A ten week sampling program of Corvallis waste treatment plant
was conducted to estimate the present levels of persistent chlorinated
hydrocarbons in influent, effluent and sludge and to develop a
method to estimate total outputs of persistent organics from a
sewage treatment plant.
Samples of influent, effluent and sludge from West Point and
Renton waste treatment plants in the Puget Sound area were analyzed
to determine levels of persistent chlorinated hydrocarbons. Some
sediment samples from this area were analyzed for the same.
38

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Analysis of sludge samples from New York Bight was initiated.
This work will be done in conjunction with experiments on fates of
barge dumping in the area.
Work Plan ZAI: Mercury, Cadmium, Arsenic, and other Heavy Metals
Distributions and Fates in Coastal Waters
1.	Dr. H. B. Mark, University of Cincinnati, EPA consultant
on instrumentation, advised National Coastal Pollution Research
Program on desirability of certain pulse height analysis equipment
in view of our trace element research needs.
2.	Experimental work on radioactive mercury uptake by crabs,
clams and oysters has been carried out in our environmental simulator.
3.	Work is continuing on a neutron activation procedure
for the analysis of taconite.
Remote Sensing
We received official notification that we were relieved of
responsibility for coordination and participation in the Bendix
and University of Michigan ERTS-A projects.
Consulting Services
Work Plan ZAR: Technical Consultation on Coastal Pollution Problems
Consultations were held with Drs. B. Brungs and D. Mount as to
what radioactive tracer studies work we could handle with our present
equipment and manpower for taconite studies. The need is for a
combined tracer and activation analysis method.
During the last quarter work has continued relative to the
effects of taconite tailings dispersal to the waters of Lake Superior.
39

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Several meeting were attended in which NCPRP personnel assisted in
the planning stages for a separate field venture to be conducted
in 1972 during which a five week shutdown of all plant operations
will occur.
Chief, Physical Oceanography Branch, attended a SEA USE Council
meeting in Seattle. A day was spent reviewing work of the
Environmental Prediction Research Facility on modeling of ocean
and near shore circulation.
Dr. Baumgartner attended a meeting of the Committee on Tidal
Hydraulics at the Waterways Experiment Station in Vicksburg,
Mississippi on June 14-15. While there he also conferred with
the Corps of Engineers Research Committee preparing a research
plan for; water quality, effects of dredging, and dredge spoils
disposal. He recommended to the Committee that the Corps of
Engineers Waterways Experiment Station conduct research on redistribution
of dredge spoils discharged to coastal waters.
Dr. Baumgartner initiated a brief study to update the
documentation of research needs in the Pacific Northwest. Douglas T.
Martin, who joined the Coastal staff June 26, will be working on this
project.
Advice on outfall models was given to the Southeast Region
personnel for use on the east coast of Florida. Several models
were explained and discussed for use in impact statements and their
evaluati on.
40

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It was learned that the State-of-the-Art report on estuarine
modeling is being used at at least seven universities as a reference or
text.
Grant and Contract Research
Work Plan ZAH: PCB, Biocides, and Other Persistent Organic Compound
Distributions and Fates in Coastal Waters
A University of California, Berkeley, grant entitled "The
Significance of Waste Water Floatables in Coastal Waters," was
approved for continuance and has been modified to include
examinations of PCB and pesticide content of surface films which
appear above ocean discharge lines. The first year's effort was
concentrated on the development of an analytical procedure for
the determination of the hexane-extractable portion of such films
and the value of such a procedure as an environmental monitoring
technique. Initial results indicate that the procedure developed
was successful; however, additional field testing is required
before a final evaluation can be made.
Work Plan XAA: Barge Dump Disposal of Wastes in Ocean and Coastal
Waters
The research grant to Tetra Tech Inc. of Pasadena, California,
is progressing on schedule and an elaborate mathematical model
should be operational by September 1972. The model will incorporate
a convective descent, a collapse, and a long term dispersion phase
into one program with considerations of ambient current and density
structures. The wastes will be characterized into several phases
(maximum 8) by density and settling characteristics, and a
resuspension capability will be available.
41

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Work Plan ZAI: Mercury, Cadmium Arsenic and Other Heavy Metals
Distribution and Fates in Coastal Waters
A grant has been made to University of Georgia, Skidaway
Institute of Marine Sciences (H. Windom, Principal Investigator)
to study the input of heavy metals in estuary regions of the
southeast coast and transport in coastal currents to marsh areas.
Work Plan DBH: Estuarine and Ocean Outfalls
The grant with the University of Washington to study the
circulation and flushing of fiords was approved and forwarded to
Washington. It has not yet been funded.
The grant with Dr. Bella of Oregon State University to study
tidal flats in estuarine water quality analysis has been completed
and the final report is being written.
The grant witn Keck Laboratory of Hydraulics and Water Resources,
under the direction of Dr. Brooks, has been completed and is being
written up in final report form.
Other
Dr. Richard C. Swartz, Biological Oceanographer, came on
board July 10. He will be stationed at Newport.
William P. Muellenhoff has been awarded a Graduate Assistant-
ship with the National Coastal Pollution Research Program for the
coming fiscal year.
Douglas T. Martin, Sanitary Engineer, joined the Coastal
Staff on June 26. He also will be stationed at Newport.
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Plans for First Quarter FY 73
Instruments will be implanted and retrieved off the Oregon
Coast for the coastal flushing and circulation project. Initial data
evaluation and processing will begin upon recovery.
The first draft of the drift flow report will be completed.
Final negotiations with the Navy regarding New York Bight
modeling will be scheduled.
As far as possible a combined tracer uptake-neutron activation
analysis methodology will be prepared for the material taconite.
Samples of effluent from various New York Bight will be studied
to evaluate heavy metal sources.
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CONSOLIDATED LABORATORY SERVICES PROGRAM
Status of Projects and Significant Accomplishments
General
The major effort has been devoted to the National Eutrophication
Survey Program in Chemistry, Computer Services, Biology and sample
handli ng.
Efforts of staff is being directed to produce a turnaround of
ten days for all analysis. In selected cases on demand a more rapid
turnaround time can be provided.
SHAVES continues to be an effective tool to assist in managing the
laboratory. A closer attention to backlog information by the staff is
assisting the manager to direct his attention to problems.
Automated Analytical Systems
Significant progress is being made on handling data from Technicon
II. The data originating from the Technicon II system goes to paper
tape and from the paper tape directly into computer storage for
report to a project leader. The necessary AQC constraints have
been programmed.
Problems still plague us in the automated Kjeldahl analysis.
Assistance has been requested from AQCL, in NERC-Cincinnati .
The arsenic interference in the phosphorus analysis has been
eliminated with the addition of meta bisulfite and thiosulfate in
the appropriate steps of the automated flow scheme.
All the Automated Systems nave been renovated in-nouse in
anticipation of the sample load from the National Eutrophication Lake
Survey Program.

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Specialized Analysis
Two systems are being used for total organic carbon analysis. The
Beckman system is being used for analysis of unpreserved samples where
the difference between time of collection and time of analysis is
short. The OIC system is capable of accepting a sample preserved
in the field with mercuric chloride or a sample sealed in the field
in an ampoule.
Work is continuing in analysis of the gases N2> 02, C02, and A2
dissolved in water. CLS has acquired a Van Slyke apparatus,
a Natelson apparatus and use of a saturometer. Comparative studies
will be made using all systems and the gas chromatographic technique.
The staff will evaluate all the techniques and report specific
details found.
Atomic Absorption Spectrophotometry
A great deal of difficulty has been experienced with the
tantalum ribbon flameless sampler. A technician was sent to
the factory development laboratory to discuss the operational
problems. The primary purpose in acquiring the instrument is
the analysis of metals in algal cells.
A more sensitive digestion technique using a heating step is
producing better recoveries and has been reconmended by AQCL as the
most suitable procedure for mercury analysis.
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Computer Services
The computer services group has been primarily concerned with
systems approach for the National Eutrophication Survey Program.
The production of STORET numbers for stations and tags for samples
has been of primary concern.
Analytical Quality
Control charts have been produced from precision and recovery
data on replicates and spikes and accuracy data from standards. The
Hewlett Packard programmable calculator is being used to produce
AQC charts.
Work has continued on placing SHAVES programs on the "Boeing
Computer," (EPA contractor). A primary high priority effort has
been placed on direct assimilation of data by the computer from
Technicon II. The prograrmiing and interface will result in saving
of time and manpower.
Biology
The bioassy toxicity studies on spent sulfite liquor are
continuing using a larval stage of an insect form for the National
Waste Treatment Program.
Routine phytoplankton samples were handled from the Eutophicati
Program.
Assistance was given to National Eutrophication Survey Program
negotiating contract on algal species identification.
Hicrobi ology
Minimum activity and only a few samples were processed during
this quarter.
46

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Accompl ishments
Completed evaluation of the use of powder pillows of MnSO^, KI
and sulfamic acid in field determinations of DO.
Designed and scheduled fabrication of sampler for use in the
sampling of tributaries for the NESP. Assisted in the tributary sampling
program for NESP. Participated in contract negotiations on algal
species identifications for NESP.
Reports, Papers, and Presentations
Krawczyk, 0. F., and By ram, K. "The Use of a Management System in
Operating an Analytical Laboratory" submitted to American Laboratory.
Griffis W. "The Chemistry of Water Pollution: Determination of
Carbon in Environmental Systems" presented at the 30th Two Year College
Chemistry Conference held in conjunction with the ACS - Northwest
Regional Meeting, 6/16/72.
Krawczyk, D. F. "Analytical Quality Control Program in Environmental
Protection Agency" presented at 30th Two Year College Chemistry Conference
held in conjunction with the ACS - Northwest Regional Meeting, 6/16/72.
Krawczyk, D. F. "Interpretation of Chemical Data" presented at
the Training Course Chemical Analysis for Water Quality, 6/29/72.
Krawczyk, D. F. "Sample Collection and Handling Procedures
presented at the Training Course Chemical Analysis for Water Quality,
6/26/72.
Krawczyk, D. F. "Protecting our Environment" presentation made
to 3rd grade class, Lincoln School, 4/03/72.
Annual Summary (FY 72)
The following is a list of samples received and tests performed

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Program
Chemi s try
Samples Tests
Mi crobioloqy	131 ol ogy
Samples Tests Samples
HCPRP
NERP
NWTRP
HTPRP
WETS
Research Total
690
3557
1635
1
380
1039
29669
5493
5
430
343
37
6263 36,636
Faci1i ties
AQC
Region
Total
158
156
1013
336
1702
8670
7590 47,344
3
170
173
270
281
551
382
Total Samples
Total Tests
8145
47,895
Plans for First Quarter FY 73
Analysis of samples from National Eutrophication Survey Program
will continue to carry number one priority.
Calibration data for Turner fluormeter for determination of
chlorophyll a^ wi 11 be obtained using test algal species from NERP program.
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