Proceedings of a Symposium
      March 10-12,1975
      Washington, DC.
U.5. Environmental Protection Agency
      Office of Water and
      Hazardous Materials
               \
           ms
             of

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 THE INTEGRITY OF WATER
 a symposium
 SYMPOSIUM COORDINATORS:
 R. Kent Ballentine and Leonard J. Guarraia
 Water Quality Criteria Staff, EPA
 Washington, D.C.
OPENING SESSION
Chairman: Kenneth M.  Mackenthun, Acting Di-
    rector, Technical Standards Division, Office of
    Water and Hazardous Materials, EPA, Wash-
    ington, D.C.
Speakers: Kenneth M. Mackenthun
  James L. Agee, Assistant Administrator, Office
    of Water and  Hazardous  Materials,  EPA,
    Washington, D.C.
  Thomas Jorling, Director, Center for Environ-
    mental Studies, Williamstown, Massachusetts
  Donald Squires, Director, State  University of
    New York Sea Grant Program, Albany, New
    York

CHEMICAL INTEGRITY

Chairman: Dwight C. Ballinger, National Environ-
    mental  Research Center,  EPA, Cincinnati,
    Ohio
Speakers: Bostwick  Ketchum,  Director,  Woods
    Hole Oceanographic Institute,  Woods Hole,
    Massachusetts
  Arnold Greenberg, Chief, Chemical and Radio-
    logical   Laboratories,   State  of  California
    Department of Public Health, Berkeley, Cali-
    fornia
  Jay Lehr,  Executive Secretary, National Water
    Well Association, Columbus, Ohio

PHYSICAL INTEGRITY
Chairman: Richard K. Ballentine, Water Quality
    Criteria Staff, EPA, Washington, D.C.
Speakers: Donald J.  O'Connor,  Professor of
    Environmental Engineering, Manhattan Col-
    lege, New York, New York
  Donald  R. F.  Harleman, Professor  of  Civil
    Engineering and Director, Parson':! Labora-
    tory  for Water Resources, Massachusetts
    Institute  of Technology, Cambridge, Massa-
    chusetts
  John M. Wilkinson, A.  D. Little,  Inc.,  Cam-
    bridge, Massachusetts
 BIOLOGICAL INTEGRITY—
 A QUALITATIVE APPRAISAL
 Chairman:  Leonard J. Guarraia,  Water Quality
    Criteria Staff, EPA, Washington, D.C.
 Speakers:  David G.  Frey,  Indiana  University,
    Bloomington, Indiana
  George Wood well, Brookhaven National Labora-
    tories, Upton, Long Island, New York
  Charles Coutant,  Oak Ridge National Labora-
    tory, Oak Ridge, Tennessee
  Ruth Patrick,  Chief, Curator  of  Limnology,
    Academy of Natural Sciences, Philadelphia,
    Pennsylvania

 BIOLOGICAL INTEGRITY—
 A QUANTITATIVE DETERMINATION
 Chairman:  David G.  Frey,  Indiana  University,
    Sloomington, Indiana
 Speakers: Ray Johnson, National Science Founda-
    tion, Washington, D.C.
  John Cairns, Virginia Polytechnic Institute and
    State University, Blacksburg, Virginia
  Gerald  T.   Orlob,   Resource  Management
    Associates, Lafayette, California
  J. P. H. Batteke, Chief, Social Sciences Division,
    Environment Canada, Burlington, Ontario

 INTEGRITY—AN INTERPRETATION
 Chairman:  Martha Sager, Effluent Standards and
    Water Quality Information Advisory Commit-
    tee, EPA, Washington, D.C.
  Ronald B. Robie, Director, Department of Water
    Resources,  The  Resources  Agency,  Sacra-
    mento, California
  Ronald B. Outen, National  Resources Defense
    Council, Washington, D.C.
  R. M.  Billings, Director of Environmental  Con-
    trol,  Kimberly-Clark, Neenah, Wisconsin
  Gladwin  Hill,  National  Environmental  Corre-
    spondent, New York Times, New York

  Following each presentation, Symposium partici-
pants were encouraged to question the speaker.
 These discussions were recorded by a professional
reporting service and appear at  the conclusion of
each paper. They have been minimally edited, sim-
ply for clarification of the spoken word in print.
                                                                                          in

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 FOREWORD
   "The Integrity of Water" results from the formal
 papers and comments presented at an invitational
 symposium by recognized water experts represent-
 ing a variety of  disciplines  and societal interests.
 The focus of the symposium was on the definition
 and  interpretation of water quality integrity as
 viewed and discussed by representatives of State
 governments,  industry,  academia,  conservation
 and environmental groups, and others of the gen-
 eral public. The symposium was structured to ad-
 dress quantitative and qualitative characteristics of
 the physical, chemical, and biological properties of
 surface and ground waters.
  It is  recognized that streams, lakes, estuaries,
 and coastal marine waters vary in size and configu-
 ration, geologic features, and flow characteristics,
 and are influenced by climate  and  meteorological
 events, and the type and extent of human impact.
The natural integrity of such waters may be deter-
mined partially by consulting historical records of
water quality and species composition where avail-
able, by conducting ecological investigations of the
area or of  a comparable ecosystem,  and through
modeling studies  that provide an estimation of the
 natural ecosystem based upon  information avail-
 able. Appropriate water quality criteria present
 quality goals that will provide for the protection of
 aqyatic and associated  wildlife, man  and other
 users of water', and consumers of the aquatic life.
 ' This  volume adds another dimension to our re-
 corded  knowledge on water quality. It brings into
 sharp focus one of the basic issues associated with
 the  protection and management of this  Nation's
 valued aquatic resource. It highlights, once again,
 our unqualified dependence upon controlling water
 pollution if we are to continue to have a viable and
 complex society.  The Congress has  provided us
 with strong and  comprehensive water pollution
 control  laws. In accordance with the advances in
 research and development and with our increased
 knowledge about the environment, these laws will
 receive further congressional  consideration  and
 modification  as appropriate.  It is  through  the
efforts of those who participated in making this vol-
ume possible that attention is focused once again on
the basic goals of water quality to support the dy-
namic needs of this generation and of others to
come.
           Douglas M. Costle, Administrator
           U.S. Environmental Protection Agency
           June, 1977

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CONTENTS
The Integrity of Water, a Symposium
Foreword
Douglas M. Costle
OVERVIEW


    The  Problem
    James L. Agee
    Legislative  Requirements
    Kenneth M. Mackenthun
    Incorporating   Ecological  Interpretation   into
    Basic  Statutes
    Thomas Jorling
    Integrity of the Water Environment
    Donald F. Squires
CHEMICAL INTEGRITY
   The Water  Environment	
   BostwickH. Ketchum

   The Chemical Integrity of Surface Water
   ArnoldE. Greenherg

   The Integrity of Ground Water
   Jay H. Lehr and Wayne A. Pettyjohn
 15
                                                 25
 33
 41
        BIOLOGICAL INTEGRITY—
        A QUALITATIVE APPRAISAL
           Biological  Integrity of Water—an Historical Ap-
           proach                                       127
           David G. Frey

           Biological  Integrity—1975                      141
           G. M. Woodwell

           Representative Species Concept-                149
           Charles Content

           Identifying Integrity Through Ecosystem Study .    155
           Ruth Patrick
                                                        BIOLOGICAL INTEGRITY—
                                                        A QUANTITATIVE DETERMINATION
 Fisheries..                         	  165
 Ray Johnson

 Quantification of Biclogical Integrity	         171
 John Cairns, Jr.

 Modeling of Aquatic Ecosystems               189
 Gerald T. Orlob

 The Watershed as a Management Concept- -      203
 J. P. H. Batteke
                                                       INTEGRITY—AN INTERPRETATION
PHYSICAL INTEGRITY
   The Effect of Hydrology and Hydrography  on
   Water Quality
   Donald J. O'Connor
   Effect of Physical Factors on Water Quality
   Donald R. F. Harleman
   Channelization
   JohnM. Wilkinson
 61
105
117
                                                          The States' View
                                                          Ronald B. Robie
A Conservationist's View
Ronald Outen
Industry's View
R. M. Billings

The Public's View
Gladwin Hill
                                                       211
                                                       215
                                                      221
                                                      227
                                                                                                       vii

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 BIOLOGICAL INTEGRITY  OF WATER-
 AM  HISTORICAL APPROACH
 DAVID G.  FREY
 Department of Zoology
 Indiana University
 Bloornington, Indiana
   To a considerable extent the topics assigned to us
 have unreasonably artificial boundaries, because an
 eeologist cannot talk about the physics, chemistry,
 or biology of water separately, nor about the quali-
 tative aspects  of  water and its biotas separately
 from the quantitative aspects. Moreover, in A meet-
 ing such as  this of  persons with  disparate; back-
 grounds and  interests, effective communication can
 be a problem. We all tend to use words that have
 special meanings within our own disciplines and we
 assume a certain understanding of premises, princi-
 ples, and laws when we use them. To make certain
 that we all are operating on the same wave length I
 shall present several principles of ecology that must
 guide our thinking about water, its management,
 and the potential effects on it of various manipula-
 tive processes, then give my own definition of the
 integrity of  water, and finally  address the topic
 assigned me. The principles to be discussed apply
 to all  aquatic systems, but  the examples I  shall
 present  will  be concerned chiefly  with lakes, as
 they, along with the oceans,  provide in their sedi-
 ments the only record of past events not covered by
 written observations or the memory of persons still
 living.
   (1) Lakes and rivers are integral parts of larger
 systems—the watersheds or catchment areas that
 are drained  by the riveni  or drain through  the
 lakes. Besides water itself, the catchment area con-
 tributes dissolved  and participate substances, both
 mineral  and  organic. In  addition, usually lesser
'quantities of various substances are contributed
 directly to the water from the atmosphere by pre-
 cipitation and  dry fallout.   Together with such
 process-regulating variables as light, temperature,
 current  velocity,  et  cetera,  these various:  sub-
 stances comprise the abiotic portion of the aquatic
 environment  and  help  control the diversity and
 abundance of aquatic organisms.
   (2) Those substances  that  are used  directly by
 aquatic  organisms and  are  necessary  in  their
 metabolism—usually   called   essential   nutri-
 ents—are recycled in  the  system  by biological
 mechanisms. Storage in living biomass, in wood or
 sediments, or in the deep water of a stratified lake
 can delay the reutilization of these nutrients for
 varying periods of time.  Because inputs and out-
 puts, including storage, are generally in balance, an
 aquatic system to remain functional requires a con-
 tinuous input of nutrients. The quantities of nutri-
 ents and other substances contributed  by a water-
 shed vary with the geological  nature  of the sub-
 strate and its  overlying  soils,  the vegetational
 cover  of the land,  and climate. Since  all of these
 tend to form regional patterns, it is not surprising
 that rivers and lakes also tend to form regional pat-
 terns or clusters in their chemistry, productivity,
 and biotic diversity.
   (3) Besides nutrients there must also be a source
 of fixed energy, mostly in organic compounds. The
 latter  derive both from  photosynthesis accom-
 plished within the aquatic part of the  system and
 from organic materials, such as leaves,  pollen, and
 leachates produced in the terrestrial  part of the
 system. In some systems, such as lakes with small,
 nonforested watersheds, virtually 100 percent  of
 the  available  energy derives from autochthonous
 photosynthesis,  whereas in other systems, such  as
 small,  headwater  streams  in  heavily forested
 regions, almost  all  the fixed energy derives  from
 organic detritus of terrestrial origin. But whatever
 its origin, the fixed  energy in organic substances is
 the driving force that enables the organisms pres-
 ent to metabolize and carry on their life processes.
 As  the energy is used in metabolism  it is trans-
 formed into heat and dissipated from the system.
 Hence, unlike nutrients, energy cannot be recycled.
 It is a one-way street, but like nutrients there must
 be  a continuous supply   for the  ecosystem to
 function.
  (4) Taking into consideration regional  differences
 in water chemistry and nutrient supply and differ-
ences between water bodies in energy  availability
and  efficiency of nutrient recycling, each aquatic
system has accumulated over time a  diversified
biota consisting  of many species of organisms ad-
                                                                                             127

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128
THE INTEGRITY OF WATER
justed to the particular  set of conditions  in the
water body in question. For purposes of analysis
and construction of models, these organisms are
often clustered into such  functional groups  as pri-
mary  producers, herbivores, detritivores,  carni-
vores, decomposers, et cetera, but all are inter-
related.  That particular species  occur in a given
lake or river is partly a matter of the species  pool of
the  region  and the  dispersal  capabilities  of the
individual species,  partly a function  of the biotic
and abiotic relationships  in  the  water body. Al-
though we consider these systems to be in a  steady
state,  intuitively we expect the biota to adjust  to
long term changes  in climate, vegetation, soil de-
velopment,  and internal trends within the system
itself,  and we also expect the system  to be able  to
accommodate  and  eventually recover from such
short term natural stresses as scouring flushouts in
rivers, episodes of  volcanism,  landslides, and  so
forth. Homeostasis is restored.
  This, to me, is what is meant by the integrity  of
water—the capability of supporting and maintain-
ing a balanced, integrated, adaptive community  of
organisms having a composition and diversity com-
parable to that of the natural habitats of the region.
Such a community can accommodate the repetitive
stresses of the changing seasons. It can accept nor-
mal variations in input of nutrients and other mate-
rials without disruptive consequences. It displays a
resistance to change and at the same time a  capac-
ity to recover from even quite major disruptions.
  My assignment is to consider what history tells
us about  the response of aquatic systems. Anything
that happened in the past is  history. Even the
words I speak become a part of history as soon  as
they are  spoken. But most of history is unrecorded
and  hence unavailable for interpretation. In the
case  of  aquatic  systems  there  are anecdotal
accounts of particular events or conditions that may
have some comparative value. There  may be time
series  of accumulated data for particular rivers  or
lakes that document what happened during these
intervals. And, in the case of lakes (and oceans), the
accumulated sediments constitute an historical rec-
ord of changing climate and  watershed conditions
and  the  integrated response of the lake  to these
changes. Where no previous studies on particular
lakes exist and likewise no isolated anecdotes about
particular  events,  the only means  we  have  of
interpreting previous conditions is from  the sedi-
ments. For rivers this possibility does not exist  at
all, as there is no long term sequential accumulation
of sediments.  Hence, here we are completely de-
pendent on the written record, except for the geo-
morphic  and hydrologic changes that can be inter-
preted from the landscape and  residual sediments
              of the valley.
                I do not intend to say much about rivers. Their
              response to  point source additions of domestic and
              industrial wastes is the establishment of a longi-
              tudinal gradient involving a succession of chemical
              processes and organisms, which for organic wastes
              is sufficiently predictive that a series of zones—the
              sabrobic  system—has been set up to help describe
              and interpret the process of recovery. Other zone
              designations have been devised for various kinds of
              industrial wastes and the responses they elicit.
                Organisms vary  greatiy.'in their  sensitivity to
              environmental  changes  accompanying pollution.
              Fishes together with a majority of insects and mol-
              luscs are most sensitive. Blue-green algae and a few
              miscellaneous  animals  from  several groups  are
              most resistant. These differences in tolerance lead
              to a greatly simplified community at the  point of
              maximum impact, with the organisms tolerating
              the conditions here often occurring in tremendous
              numbers, and then to a gradual buildup in diversity
              of species and equitability in numbers of individuals
              downstream. Various diversity indices have been
              proposed to help quantify these changes. Diatoms
              are particularly useful in stream studies and their
              truncated log-normal distributions  are useful in
              assessing the severity of pollution. The experienced
              investigator can often determine quite easily from
              the macroinvertebrates present what the stage of
              recovery is, and can also detect residual effects of
              pollution, as from lead mines in Wales, that are no
              longer detectable chemically.
                Lakes are fundamentally different from rivers in
              a number of respects that affect the mtegrity of
              water as I have defined it. In the first place, their
              water movements are not gravity-controlled, unidi-
              rectional flows which continually flush out the chan-
              nel with  new water from above, but rather wind-
              induced circulations. Typically  in summer, when
              the wind is  not  adequate to overcome the differ-
              ences in  density set up by surface warming, the
              lake becomes divided into an upper circulating epi-
              limnion and  a lower zone,  the hypolimnion, cut off
              from the  surface by a steep density gradient and as
              a result subject to generally much  weaker water
              movements than the epilimnion. During periods of
              calm weather those lakes that circulate continu-
              ously   over   summer  can  become  temporarily
              stratified and even the epilimnion of the others can
              develop secondary stratifications under these cir-
              cumstances. Regardless of the  duration  of such
              stratification, the hypolimnion, or its equivalent in
              temporary  stratification,  experiences  cumulative
              chemical  changes,  most important of which is the
              gradual depletion of dissolved oxygen by biological
              activity.  The longer the duration of stratification

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                           BIOLOGICAL INTEGRITY—A QUALITATIVE APPRAISAL
                                              129
  and the greater the amount of biological activity,
  the more severe will be the oxygen depletion with
  its attendant stresses  on organisms requiring cer-
  tain levels of dissolved oxygen for their survival.
    Unlike rivers, lakes accumulate sediments pro-
  gressively and sequentially. One effect of these sed-
  iments is gradually to reduce the volume of the
  hypolimnion over time and hence the total volume
  of dissolved oxygen it contains when stratification
  becomes established in spring or summer. Conse-
  quently, even without any increase in  biological
  activity, the hypolimnion will experience a gradual
  reduction in oxygen concentration over time, which
  brings  about the extinction  and replacement of
  various deepwater organisms as their tolerances
  for low oxygen are exceeded.
   The sediments constitute a storage for energy
  and nutrients. Some ol this is utilized by bacteria
  which can continue  their activity even to consider-
  able depths in the sediments, or by various animals,
  which because of their need for molecular oxygen
 are confined generally to the uppermost few centi-
 meters. Whether the  sediments are functioning
 chiefly as a  sink or as  a reservoir for nutrients is
 important in problems concerning  eutrophication
 and its management.
   The sediments also constitute a chronolojjical rec-
 ord of processes in  the lake and conditions in its
 watershed, including climate. A perceptive reading
 of the record—its chemistry,  physics, and paleon-
 tology—gives  us much  insight into the stability of
 lake systems when  subjected to various stresses,
 including those resulting from man's activities, and
 their rates of recovery.
  A third major difference between rivers and
 lakes is that the water  in lakes has  a certain  resi-
 dence  time, up to 100 years or more in some of the
 large  lakes,  determined by the relationship be-
 tween the input of water from the catchment  area
 and direct precipitation  and the total volume of the
 lake. This allows for the recycling of  nutrients in
 the same place, subject to the constraints imposed
 by stratification, and the buildup of a diverse com-
 munity of small floating organisms—the plankton.
 And even apart from any  storage function of the
 sediments, the residence or  replacement  time
 means that there is an inherent lag in response of
 the system to any increase or decrease in inputs of
 nutrients or  other  substances having biological
 effects. In streams the  response  to input changes
 can be almost immediate. Any storages in the sedi-
 ments are mostly temporary, as the sediments can
 be swept downstream during the next flood stage.
  WTiat I should  like to do now  is present a  few
examples of the kinds of responses made by lakes to
various stresses.
    It was almost axiomatic in limnology until quite
  recently  that lakes increase in productivity over
  time through natural causes,  a process that has
  been  termed  natural  eutrophication. This  idea
  seemed to be substantiated by some early studies in
  paleolimnology which showed that the organic con-
  tent of the sediments increased exponentially over
  time from a very  low  level initially to a certain
  plateau level—the trophic equilibrium—which was
  then  maintained .essentially unchanged  almost to
  the present. The trophic equilibrium was regarded
  as a state in which production was no longer limited
  by  nutrient supply but rather by such factors as
  light penetration that affect the efficiency of utiliza-
  tion and recycling of nutrients within the system.
   The sedimentary chlorophyll  degradation prod-
  ucts (SCDP) in sediments originate almost entirely
  from  photosynthetic plants, chiefly algae, in the
  lake itself. Present evidence suggests that these
  organic compounds are  relatively stable in sedi-
  ments. Hence, the quantitative changes over time
  of these  substances can give an indication of the
  magnitude and changes in productivity experienced
  by a lake. One core from Pretty Lake, Ind., (Figure
  1), shows low SCDP and hence low productivity in
  late glacial time and then an exponential increase to
 a maximum, maintained essentially at plateau level
 almost to the present. This corresponds  to  the
 classical interpretation of the trophic equilibrium in
 lake ontogeny. But the second core from shallower
 water shows a decline in SCDP after the maximum
 following the exponential increase, which does not
 fit the model.
   We now know from  this and other studies in
 paleolimnology that change  in productivity over
 time is not unidirectional from low to high in all
 lakes, but that some lakes had a period of high pro-
 ductivity  initially and then became less productive
 subsequently.  Others  had  discrete episodes of
 higher  productivity  from  whatever  cause.  For
 example,   Lake  Trummen  in  southern  Sweden
 (Digerfeldt, 1972} had high accumulation rates of
 organic matter, nitrogen, and phosphorus at the
 beginning of postglacial time approximately 10,000
 years  ago. These subsequently  declined and re-
 mained low up to very recent time, when industrial
 organic effluents completely changed the character
 of the lake (Figure 2).  These relationships are
 interpreted as reflecting the high early availability
 of nutrients from the youthful soils of the regional
 till sheets, with the subsequent decline resulting
 from the progressive impoverishment of the soil by
 leaching and by the reduction of subsurface inflow
into  the lake as basin-sealing sediments accumu-
lated.
  Hence, the productive status of a lake is depend-

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  130
THE INTEGRITY OF WATER
  2000
  10OO
  6000
- 80OO
  10000
  12000
  14000
                 so          100
                 SCDP/G  ORGANIC  MATTER

                      FIGURE 1
  ent on the magnitude of its nutrient inputs, subject
  to the internal controls of the system. If we can de-
  crease the nutrient supply, we can expect a more or
  less commensurate decrease in productivity. Vari-
  ous attempts are being made to model the magni-
  tude of the response expected from any reduction in
  nutrient loading, but the rate of response  is still
  unpredictable. The rapid reduction of phosphorus
  and productivity in Lake Washington following the
  elimination   of   secondary   sewage   effluents
  (Edmondson, 1972) is encouraging, although some
  other components of the system,  such as nitrogen,
  did not behave  in the same dramatic way. Other
  examples to be presented suggest that the response
  time of the total system, or perhaps better the re-
  bound time from a stressed condition, can be much
  longer than in Lake Washington.
    The responses of a lake to the decreasing oxygen
  concentration of the hypolimnion  over time are in-
  structive and significant. Western Lake Erie is so
  shallow that it stratifies only  temporarily in sum-
              mer  during calm weather. Already by 1953 the
              oxygen demand of the sediments had become such
              that during a brief period of temporary stratifica-
              tion in late summer the oxygen content of the water
              overlying the bottom was sufficiently reduced to
              cause the wholesale death of the nymphs of the bur-
              rowing mayfly, one of the most abundant organisms
              here and a very important fish food {Britt, 1955).
              The mayflies never reestablished their populations
              but they have been replaced by smaller oligochaete
              worms capable of enduring quite  low  concentra-
              tions of dissolved oxygen. Thus, a single event, al-
              though obviously with antecedent conditions, led to
              a complete  change  in one portion of  the biotic
              community.
                The cisco is another case in point, although per-
              haps less spectacular.  If we want to talk about
              endangered species, or at least endangered popula-
              tions, this is one. It is a fish that lives in deep water
              with  requirements for both low temperature and
              high oxygen. If either of these limits is exceeded,
              the fish perishes. As the summer oxygen concen-
              tration of the hypolimnion gradually decreases over
              time, the cisco, in order to meet its oxygen needs,
              is forced upward into strata  with progressively
              higher temperatures.  Eventually the combination
              of low oxygen in deep water and high temperatures
              toward the surface eliminates the habitat suitable
              for the cisco and the population is extinguished. In
              1952 Indiana had 41 lakes with known cisco popula-
              tions (Frey, 1955a). It is certain that a  number of
              these populations have been completely  extirpated
              since then, and it is not at all certain how long the
              others will survive.
                The species of midge larvae associated with deep-
              water sediments have different requirements for
              dissolved oxygen, so that as the oxygen content of
              the hypolimnion gradually declines over time, the
              composition of  the  midge  community  likewise
              changes progressively in favor of species capable of
              tolerating lower oxygen concentrations. This led
              early in limnology to the establishment of a series of
              lake  types  based on  the dominant species of off-
              shore midges and presumably representing stages
              in a successional series.  Fortunately the head cap-
              sules of the midge larvae, which are well preserved
              in lake sediments, suffice to identify the organisms
              to the generic and sometimes to the species levels.
              In general,  the pattern of succession in an  in-
              dividual lake corresponds to the  model, with spe-
              cies requiring high levels of oxygen occurring early
              in the history of the  lake; these subsequently are
              replaced  by species  more  tolerant of  reduced
              oxygen;  they in turn are replaced  by species still
              more tolerant, and so on until the only species left is
              a mosquito-like larva Chaoborus, which can endure

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BIOLOGICAL INTEGRITY—A QUALITATIVE APPRAISAL
131
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132
                                    THE INTEGRITY
 anaerobiosis for a while, but eventually even it is
 eliminated if conditions continue to deteriorate
   The most incisive study to date is that of Hot-
 mann (1971) on Schohsee m northern Germany. A
 midge community associated with moderate oligo-
 trophy  dominated the offshore  community  until
 early sub-Boreal time about 1500 B.C. IMS was r-
 lowed by a  transitional community las ing perhaps
 2,500 years, and this in turn by  a eutropinc com-
 munity for the last 1 000 ^^^^
 much more  complex than indicated by this too briet
 summary in that throughout the 10,000 years of
 lake history there were migrations of originally
 shallow-water  species into deep  water  extinction
 of deepwater species, and successional  dominance
 of one  species or another as conditions graduaHy
 changed. Actual quantitative studies of the benthos
 in 1964-67  compared with similar studies m 1924
 show that the populations are still changing (F.gure
 3) . In this  interval the populat.on of chironomids,
 especially  Chironomus, has  declined drastically,
 being replaced by an increasing population of ohgo-
 chaetes. Chaoborus remained *«^"»-£*
 situation is reminiscent of  western Lake  Erie,
 where oligochaetes took over after the big killof f of

 m S^tJerTfir^moved into the Bay of Quinte region
 of Lake Ontario about 1784  Government reporte
 describe the devastation of thousands of acres by
 lumbering  and the erosion problems resulting. The
 initial  impact  of this land disturbance on the Bay
 was to change the deepwater sediment from silt
 dominance to  clay dominance and to bring about a
 marked decrease in organic  content through ddu-
 tion by clay (Warwick, 1975). Subsequently, the or-
 ganic  content increased gradually,  although it  is
 still less than pre-impact level, but now there is a
 pronounced decline in oxygen content of the deep
  water in summer. The initial response of the midge
 community was  somewhat surprising; it becam
  more oligotrophic than it had been before but then
  it proceeded through several successional Phases to
  a quite strongly eutrophic stage at present (Figure
  4). Unlike previous investigators, Warwick pe-
  lieves that the earliest stages in midge succession
  are controlled by food supply  more than by the
  minimum  annual concentration of oxygen  m the
  hypolimnion.  The latter * important chiefly m the
  later stages of succession. Besides the shift in Jithol
  ogy from  silt to clay, the sediments deriving ^rom
  the impact period are marked by the appearan. e ol
  the pollen of Ambrosia (ragweed), the abundance of
OF WATER
America for forest clearance and the initiation of
      Qfect on our water resources is nothing re-
  ^. ™   5  fa     Uen diagram Of Langsee in
     J^ff^T (F?ey  1955b), a lake that pres-
southern Austria ^y  ^  ^            ^

jf^j^ti^lte of the rest of the
, " F.  *JL_ and autumn-a condition known as
lake m spnng «,d ^umn^      &        ^
part al c™^™™    M h fa obvious in the dia.
^^e ^esTde5; changes in the non-tree pol-
gra  'inciudjng  the appearance of various agricul-
lens  ncin            g^ of such cultivated
                          , as well as a disruption
                              vegetation. At this
                   bands of clay Star, separated
          reduced sediment completely unlike the
                    sited prior to this but identical
                     Quite obviously, this is when
                        region about 2,300 years
                         ^ clearance of the land
                                 fc ^

                    lake, triggering the condition
 of pi? acTrculation, now maintained by biological
 «par         the sudden import of large amounts
 means. «*J»^ can haye ^ferent consequences
                    i a small volcanic lake in cen-
                   km from Rome, had an initial
             of   ^t^ty  when  formed about
                * then &       hase of ,ow prod c.
 £>»" 'T   ^^      when the construction of a
 « _y ^  v^ Cassia  ^ m  R  c   compieteiy
      •          t rf nutrients and other substances
      ^ ^^ ^atershed (Hutchinson, et al. 1970).
 ^ ]ake re    ded by dramatic increases in pro-
 ductivit  and sedimentation  rates  which did not
      unta ^^^ 1QQQ years after the disturbance
 [pi     6)   Since tnen, productivity, as inferred
 j^ the accumulation rates of SUch substances as
 oreanic matter, nitrogen, et cetera, has subsided to
   B   ^ much      ter than that  existing before
      disturbance. The lag in response  and the long
 duration Q{ the response are probably related to the
          Monterosi is a closed basin with no perma-
      streams draining its very small watershed and
 withoutputoniyvia seepage.
                         lafee fa northern Gemany
    Gros ser no             ^ ^^ ^      d

  tamo           Thienemann and his associates. In
  thereby ^ gu^ ^^^ ^ ^ ^ raised

  i                            overflooding much
                                             the


-------
                BIOLOGICAL INTEGRITY—A QUALITATIVE APPRAISAL
                     SCHOHSEE BENTHOS 1924 AND 1964/67
133
 NUMBERS/I^
103
1fl2
I01
P
1924
-
	 1

12-
1964/67
l£lm
UHIHUNOF

WIDAE/CHIRO
1924

DEI
1964/67
^^^^^™
24m
>TH
NOMUS

1924
>2t

1964/67
lm
           CHIRONOMIDAE



           CHIRONOMUS
NUMBERS/M2
 102  _
 101
 10* E
 10*
                          CHAOBORUS FLAVICANS
                                                         1964/67
=
IERS/M
1924
12-1
2
1964/67
9m


1924
20-
DEF
1964/67
24m
•TH


1924
>2

4m


•
-
12-1
^"
9m


TUBIFI
1924
^•^^^-™
20-:
DEP
CIDAE
1964/67
^^_^^_
24m
TH


1924
^•^MBI^H
>2'
^_.«
Im



-------
134
                                      THE INTEGRITY OF WATER
           PHASES IN THE GL.ENORA-B  CORE
UJ
u.
o
UJ
0-
5-
IO-
15-
20-
25-
30-
35-
4O-
50-
S5-
90-
115-
140-
164-
Ihe MODERATELY
EUTROPHIC
PHASE
The MESOTROPHIC
PHASE
Ihe IMBALANCED
OLIGOTROPHIC
PHASE
Ihe INITIAL IMPACT
PHASE
Ihe PRE -SETTLEMENT
OLIGOTROPHIC
PHASE
Cr.ifprtofnus spp
PfOCtOdjyS Is S 1 SPP
Phqenppwclfo cl corrKitip ( Ztir }
Bfjir.o sp Jfingjfajcfl ly&e
Sv/icfl^octajius ^gmifirers Hit;',
CfiirtMorm* spp
Aficrppjtfil^) SPP
Toriylorsini Type H
'>itWfiidJ 4Z«rf J
At^npd'OmfSQ depecfinow Saftrn
WoJC'Olriiloclodivs cfiang' Scelli
Poroctotfopcl/np SP gb^cum Jype
Phaeflopsgc^o cf cflfpcino I Ze;i )
                      FIGURE 4
                                     mm per year at the beginning of postglacial time to
                                     only about 0.5 mm per year 9,000 years later, sud-
                                     denly jumped 20-fold to more than 10 mm per year.
                                     This has resulted in half the 15 meters of sediment
                                     in the lake deriving from only the last 700 years of
                                     the lake's existence. The big increases at this time
                                     in  the accumulation rates of such substances as
                                     zinc, copper,  cobalt, and aluminum reflect the in-
                                     creased input of mineral substances to deep water
                                     from the overflooded land and probably from the
                                     watershed' also. Correspondingly big increases in
                                     the accumulation rates of  organic matter, chloro-
                                     phyll derivatives, and diatom silica reflect the big
                                     increase in production within  the system resulting
                                     from this changed regime (Figure 7).
                                        The lake level had been raised to power a mill
                                     dam, as was common in northern Germany at this
                                     time,  and also to facilitate the production of eels,
                                     but the concomitant flooding of valuable agricul-
                                      tural lands resulted in a long-continuing strife be-
      BLACK REDUCED


      SAPROPEL a TH
        CD      10 5  5      2O


             MIXED OAK  FOftEST


GYTTJ6 • SAPROPEL


ICK CL1T
i  Mtromictlc Phase
STABLE FIN6LV-lAtll»*TE[> 6TTTTJA


SILT
                                                Halomictic Plait
                                                FIGURES

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                           BIOLOGICAL INTEGRITY—A QUALITATIVE APPRAISAL
                                             135
                                                                              EStiM«T(0»»'[ 0>
                                                                              OF ic*iiABi.c ORGAN
                                                                              10TTED ACA.NSIOE
                                              FIGURE 6
 tween the mill operators and fishermen on the one
 hand and  the  manor owners and farmers on the
 other. Finally,  in 1882 the lake level was lowered by
 1.14 m. Up to  this time the accumulation rates of
 most mineral substances had been declining irregu-
 larly, and likewise the indicators of biological activ-
 ity. The sudden  lowering in lake level resulted in
 the erosion and  deposition offshore of sediments
 that had accumulated in shallow water, yielding a
 discrete  horizon  of coarse-grained sediments  and
 associated sharp  peaks of various mineral constitu-
 ents. Accumulation rates of chlorophyll derivatives
 and diatom silica declined  at  this time, perhaps
 through light limitation of production by increased
 inorganic turbidity.  The large increase in chloro-
 phyll  derivatives in very  recent time, reflecting
 high productivity, is attributed to the heavy use of
 agricultural fertilizers and phosphate detergents
 and to the draining of the  surrounding wetlands.
 Such an increase of organic matter and other indi-
 cators of production toward the surface is common-
 place  among lakes being  stressed  by man, fre-
 quently resulting in a completely different type of
 sediment than anything deposited earlier.
  Grosser Plo'ner See is but one of a number of in-
stances where the productivity of a lake has been
markedly increased by raising its water level. The
present high productivity of Grosser Plb'ner See is
shared by  many lakes  of  the  region,  all accom-
 plished within the past few decades in direct re-
 sponse to man's increasing impact on the systems.
 Ohle  (1973) has used the term "rasante Eutro-
 phierung" (racing eutrophication) for this rapid re-
 sponse of lakes to cultural influences, in contrast to
 the generally slow, balanced development occur-
 ring naturally.
   The most abundant animal remains in lake sedi-
 ments are the exoskeletal fragments of the Clado-
 cera,  particularly  the family  Chydoridae (Frey,
 1964). They are abundant enough for the construc-
 tion of close-interval stratigraphies similar to those
 of pollen and diatoms and for the calculation of vari-
 ous diversity indices and distribution  functions.
 Since  the deepwater sediments represent an inte-
 gration over time and habitat, the population of re-
 mains recovered from the sediments is partly artifi-
 cial, in that all the species represented probably did
 not co-occur at the same time and place. Yet the di-
 versity indices of the chydorids do show certain
 demonstrable relationships to such  variables as
 productivity and transparency  and,  as  shown in
 Figure 8, the relative abundance of the various spe-
 cies in an unstressed situation conforms almost pre-
 cisely to the MacArthur broken stick model for con-
tiguous  but  non-overlapping   niches   (Goulden,
 1969a). Hence, the species distribution predicted by
this model can be used to assess the extent of imbal-
ance in the system.

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136
                                        THE INTEGRITY OF WATER
   15 .
                       g AI/m2«Q




     0      5      tt      15      20      25      30
    5 _
 I
   10 „
   15 _
                                                             15.
                                                                             gDiot Si02/n/«o
g Chlorophyll/
                                                  FIGURE?

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                         BIOLOGICAL INTEGRITY-A QUALITATIVE APPRAISAL
                                                                                             137
  In a series  of 21  lakes in Denmark for  which
measurements of annual phytoplankton photosyn-
thesis  by  radiocarbon  uptake   are  available
(Whiteside, 1969), there is; a direct relationship be-
tween species diversity and transparency and an in-
direct relationship between species diversity and
productivity. There is also an inverse relationship
between transparency and productivity. The inter-
pretation of these relationships is that as lakes be-
come more productive, they become less transpar-
ent from the development of larger phytoplankton
populations, and with higher productivity the chy-
dorid community is  thrown out of balance, quite
possibly  from  a reduction  of habitat diversity
through the curtailment of species diversity and
areal extent of the aquatic plants which form the
major habitat of the chydorids. And since the chy-
dorids are but one component of a complex commu-
nity, one can assume that the community as a whole
has been stressed by an increase in productivity.
  In another study in Denmark,  Whiteside (1970)
attempted to establish the  predictive value  of
chydorid  communities  for  lake  type, and then
attempted to use  these results  in interpreting
changes in lake type in postglacial time in response
to climate and vegetational patterns of the  water-
sheds. A hard water, eutrophic lake  (Esrom Srf)
was sufficiently buffered internally that it went
placidly about its business during postglacial time
almost regardless of external stresses  that would
be expected to have repercussions on  the system,
whereas  a soft  water,  oligotrophic lake  (Grane
Langstf) reacted nervously to even  small external
stresses. Thus, the response to a  given stress can
be expected to vary greatly from lake to lake de-
pending on its particular suite of ecological condi-
tions and balances.
  The MacArthur predictive model has been used
to assess community stresses resulting from the
rapid climatic  change of  the  last  interstadial
(Goulden, 1969a), from episodes of Mayan agricul-
ture in Central America (Goulden, 1966), and from
volcanic ash falls in a lake in Japan {Tsukada, 1967).
The last  study (Figure 9) is  interesting in showing
that a single instantaneous  but massive perturba-
tion, as from an aslifall, can  have marked and long-
lasting effects on the community  structure of a
lake.
  There  are  quite a few other studies on the re-
sponses of lakes to stresses that might be cited, but
I should like to give just one  more. The paleolimnol-
ogy of North Pond in northwestern Massachusetts
is being studied intensively by  Tom Crisman, a
graduate student at  Indiana University. Many ma-
jor changes, almost as precipitous as those in Gros-
ser Ploner See,  occurred in the lake shortly after
the pine forest represented by pollen zone B was re-
placed by deciduous hardwoods. Productivity in the
lake, as  evidenced by the quantity of chlorophyll
derivatives in the sediments, increased dramatic-
ally at that  time, along with nitrogen and  phos-
phorus. A species of  planktonic Cladocera, Bosmina
coregoni, which is usually considered characteristic
of more oligotrophic situations,  was  replaced al-
most instantaneously  by  Bosmina longirostris,
characteristic of more eutrophic situations (Figure
10). Since there is no clear  evidence for any major
fluctuation in water level and since it is unlikely the
Amerindians could have modified the watershed to
any appreciable extent, the only correlate and pos-
sible cause is the shift in forest composition. But
this is difficult to reconcile  with  the data, because
watershed studies to date have demonstrated that
deciduous forests are more parsimonious than co-
niferous  forests in  releasing nutrients from the
system.
   Let me attempt to summarize some of the major
points developed. Lakes change biologically during
their existence  from changing inputs of nutrients
and energy and from changing internal control
mechanisms, associated in  part with stratification
and depletion of oxygen content in deep water. The
biological changes in many instances have been
 gradual, although in others  they have been sudden,
 associated   with  natural   catastrophes,   major
 changes in water level, or even changes in the dom-
 inant vegetation type in the watershed.
   Lakes vary in their sensitivity to external stress
 and in their rapidity and magnitude of response.
 Man's chief  impact  is to stress the systems so se-
 verely that they are thrown out of balance and the

-------
 138
                        THF:
                                                     OF WATER
           I   2349   10 13
               SPECIES FJNK.
                       •J  -4  .6  .B "7.0  10X SO  50   70   90  0  20 40  60*loi/c~
                            coo
PERCCTT  Of

   FICI.-RE 10
                         TOTAL BOSWNi
rate of change is accelerated—what  Ohle calls
"rasante Butrophierung." Both for  natural  and
man-induced stresses, the response of the total sys-
tem may be fast or slow, and likewise the rate of re-
 covery. The lag may be considerably greater than
 predicted  from  the  water  replacement  time,
 amounting to hundreds of years even fn small lakes
 if our examples from the past have  been correctly
 interpreted. Hopefully, the response time,  particu-
 larly of recovery, wall be fairly short, but faced with
 the  unpredictability of  the  response  time,  we
 should be much more solicitous about the stresses
 placed on our lakes, as even with massive engineer-
 ing input they may not recover as rapidly as hoped.
   Eutrophication occurs naturally, but so does the
 contrary process of oligotrophication.  That is,  a
 lake can become less productive with time, if its nu-
 trient budget is decreased, Paleolimnology has not
 yet been able to resolve what the major controls of
 productivity have been in the past for any particu-
 lar lake, except by inference from our knowledge of
 present controls.  But since phosphorus, more than
 any other single substance, is the dominant control
of productivity in temperate lakes, it is essential to
 keep phosphorus  inputs at. a minimum if we are to
have any hope at all of maintaining the integrity of
our lakes.

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                             BIOLOGICAL INTEGRITY—A QUALITATIVE APPRAISAL
                                                                                                       139
^REFERENCES

  Hritt N. W. 1955. Stratification in western Lake Erie in summer
   of 1953: effects on the Hexagenia (Ephemeroptera) population.
I'  Ecology 36:239-244.
  Dieerfeldt, Gunnar. 1972. The postglacial development of Lake
   Trununen. Regional vegetation history, water level changes
   and paleolimnology. Folia Limnol. Scandinavica 18:1 104.
I Mjjjondsion, W. T. 1972. Nutrients and phytoplankton in Lake
   Washington. Pages 172-188 in G. E. Likens, ed. Nutrients and
   eutrophication. Amer. Soc. Limnol. Oceanogr.  Spec. Symp. It
   x, 328 pp.
  Frey. D. G. 1955a. Distributional ecology of the cisco (Coregonus
   artedul in Indiana. Invest.  Indiana Lakes & Streams 7:177
   228.
  _,	.. iii55b.  Langsee: a history of meromixis.  Mem. 1st, Ital.
   Idrobiok Suppl. 8:141-164.
  ';	     1964. Remains of animals in Quaternary lake and bog
  - sediments and their interpretation. Ergebnisse der Limnolo-
   gie2:l 114.
   .	. 1974. Paleolimnology. Mitt. Internal. Verein. Limnol.
   20:95 1£3.
 :<3oulden, (!. E. 1966. La Aguada de Santa Ana Vieja: an inter-
   pretative study of the cladoceran microfossils. Arch. Hydro-
   biol. 62:5(73-405.
        19<>9a.  Interpretative  studies of cladoceran microfossils
   in lake s-jdiments. Pages 43 55 in D. G. Frey, ed. Symposium
   on paleolimnology. Mitt. Internet. Verein. Limnol. 17:1-448.
   __, I909b.  Temporal changes in diversity. Pages 96-100 in
   6. M. WoodweU and H. H. Smith, eds. Diversity and stability
   in ecological  systems. Brookha-'en Symposia  in  Biology, 22:
 !-yi!.264P3.
 •jBWmann, Wolfgang. 1971. Die postglaziale Entwicklung der
   phirunonriidenund Chaoborus-Fauna (Dept.) des Schohsees.
 ' .Arch. Hy irobiol. Suppl. 40(1/2): I 74.
  flutehwson, G. E. et al. 1970, lannia: an account of the history
   ;"«ad development of the Lago di Monterosi, Latium  Italy.
 V ''lt*aa. Ainer. Phil. Soc. N.S. 60(4):1 -178.
  !OfcJe, Walcemar.  1972.  Die  Sedimente des Grossen Ploner
   •8ees als .Dokumente der Zivilization. Jahrb. f. Heimatkunde
   (Him) 2:7-27.
  gfc . 1973. Die rasante Eutrophierung des Grossen Ploner
   •Sew in J-Yiihgeschichtlicher Zeit.  Die Naturwissenschaften
   iHl):47.
         Matsuo. 1967.  Fossil Cladocera  in Lake Nojiri and
               r. Quaternary Res. 6(3):101  110. In Japanese.
        . Bill.  1975. The impact cf man on the  Bay of Quinte,
   [ask* Ontiirio, as shown by the subfossil chironomid succession
   tttdronociidae, Diptera). Proc. Int. Assoc. Limnol. Vol. 19. In

         , M. C. 1969. Chydorid (Cladocera) remains in surficial
          ist in Danish lakes and their significance to paleolimno-
        interpretation. Pages 193-201 in D. G. Frey, ed. Sym-
          on paleolimnology. Mitt.  Internal. Verein. Limnol.
        M8.
        1970. Danish chydorid Cladocera: modern  ecology and
    118.
     HUSSION
    Cottai-ent: Your definition of the integrity of wa-
            to be the capability of maintaining and
              a composition of organisms  that can
     t i& its natural state. In your discussion you de-
         Varying natural states and change of proc-
ess. How does that translate to a useful definition
today?
   Dr. Frey: We had an example a couple of weeks
ago when two young Ph.D.'s who were modeling
ecosystems gave seminars at Indiana University.
They had linear models which didn't allow for any
change over time. However, in any particular lake
there will  be  changes  over  time,  induced  by
changes in climate  and  vegetation,  soil  develop-
ment, and  so forth.  The response of the aquatic
community to these'changes will probably be adap-
tive adjustments in species composition and in the
relative abundance of species, controlled in part by
the mobility of the species. I think a definition of in-
tegrity has to include the concept of a balanced, in-
tegrated, adaptive community.
   Comment: Did you go into these?
   Dr. Frey: No, they are objectives.
   Comment: Have you made any comparisons with
other organisms over an historical period? Would
you be able to use changes in the plankton popula-
tion to .detect changes in the water, as you pointed
out is possible  with fish  populations? Would  the
changes be subtle or quite apparent?
   Dr. Frey: I  didn't go into any of the long term
studies, because even the best of these are less than
100 years old. I know,  for example, that there are
records for the Chicago water supply which docu-
ment the kinds and quantities of plankton in south-
ern Lake Michigan over many decades. Probably
the longest and most nearly continuous record of all
is that for  Lake Zurich in Switzerland. Here  the
deepwater  sediments have  been  accumulating as
discrete annual layers since the late 1800's. As the
lake became more eutrophic under man's influence,
various species of algae invaded the lake and devel-
oped to bloom proportions. These are documented
by studies  of  the   plankton.  Significantly,  the
blooms of the  various species, particularly the dia-
toms, are also recorded in the appropriate annual
layers, so that Lake Zurich constitutes to some ex-
tent a calibration system for the interpretation of
real events and changes in a lake from what is re-
coverable from the sediments.
   Most of these long term data series have been re-
ported elsewhere at various times. I didn't attempt
to summarize them, but instead concentrated on
the kinds of interpretations that can be made from
the sedimentary record.
   Comment: I'd like to ask you a philosophical ques-
tion stemming from your definition of integrity. In
your opinion,  are efforts to reverse a naturally oc-
curring trend toward eutrophication counter to the
integrity of that lake?
   Dr. Frey: I had to leave out a number of pages of
my prepared text because of time limitations (but

-------
140
                                     THE INTEGRITY OF WATER
these are included in the published paper). For the
chydorid cladocerans, which are well represented
in lake sediments, the species diversity of the com-
munity declines as the productivity of the lake in-
creases,  indicating  that  the system  is  being
stressed. This should not be interpreted to mean
that Jill productive lakes are out of balance because
the rate of change is probably the important consid-
eration. Where the increased productivity is the re-
sult of man or of some  essentially  instantaneous
event such as a volcanic ash fall, the rate of change
in nutrient budgets or other environmental condi-
tions is so great that the community cannot keep
pace with orderly and adaptive adjustments. But
where the forcing variables change slowly over
time the aquatic biota is able to maintain an internal
balance. Hence, I  am in favor of either reversing
the trend toward increasing productivity in our nat-
ural waters,  except where this is specifically de-
sired, or at least sufficiently reducing the rate at
which eutrophication is occurring so that the sys-
tem is not stressed unduly.

-------