&EPA
www.epa.gov/airscience
science   in   ACTION
                                                                CLEAN AIR  RESEARCH
                                                                PROGRAM
      AIR POLLUTION RESEARCH ADDRESSES RELATIVE
      TOXICITY OF DIFFERENT PARTICLE SIZES
      Issue:
      Outdoor air pollution particles are
      currently divided into three
      classes based on their size:

      • Coarse particulate matter (PM)
        consists of particles with a
        diameter between 2.5 and 10
        micrometers (|im) and deposit
        efficiently along the airways.
        Particles larger than 10 jam are
        generally not inhalable into the
        lungs.
      • Fine PM consists of particles
        with a diameter less than 2.5
        Jim and can be inhaled deeply
        into the lungs.
      • Ultrafme particles, the smallest,
        consist of particles with a
        diameter smaller than 0.1 jim
        and  have widespread deposition
        within the respiratory tract.

      It is not well understood whether
      particles with different size
      ranges have different abilities to
      cause adverse health effects.
             The U.S. Environmental
             Protection Agency currently
             regulates PM on the basis of mass
             in both the fine and coarse size
             ranges. More than 150
             epidemiology studies have
             demonstrated an association
             between fine PM and acute
             mortality and morbidity.

             A smaller number of studies have
             linked exposure to coarse PM
             with increased mortality and
             morbidity. However, uncertainty
             about the effects of coarse PM
             derived from different sources has
             led EPA to ask for science to
             address the question of
             differential toxicity from coarse
             PM of urban areas versus PM of
             rural areas. There is also concern
             that because of the high
             concentration of biological
             compounds present in coarse PM,
             compared with smaller size
             fractions, asthmatics may be a
             particularly susceptible
subpopulation to coarse particles.

In addition, there is concern that
there may be adverse health
effects associated with exposure
to ultrafine particles, which, if
established, could potentially lead
EPA to propose a a PM standard
for these smaller particles.

Because of these uncertainties,
regulators and scientists both
believe there is more to learn
about the adverse health effects of
different sized PM, especially as
the composition of these particles
varies.

Scientific Objective:
The Clean Air Research Program
in EPA's Office of Research and
Development is conducting a
comprehensive and integrated
research program to compare the
cardiovascular and pulmonary
responses of humans or
appropriate animal models to
different sizes of PM. Both EPA
                                                                                   continued on back
       U.S. Environmental Protection Agency
       Office of Research and Development

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  &EPA
www.epa.gov/airscience
science   in   ACTION
       CLEAN  AIR  RESEARCH  PROGRAM
       continued from front
       scientists as well as academic
       researchers funded by the Agency
       are engaged in these studies.

       The research program includes
       studies which:

       •  Compare the cardiovascular
         and pulmonary response of
         healthy human volunteers or
         animal models exposed to
         coarse, fine, and ultrafme
         particles.
       •  Determine if people with pre-
         existing cardiovascular or
         pulmonary disease such as
         asthma are especially
         susceptible to one or all of the
         particle sizes.
       •  Compare the cardiovascular
         and pulmonary response to
         coarse PM originating from
         urban and rural areas.
       •  Identify the underlying cellular
         and molecular mechanisms by
         which each size fraction causes
         adverse health effects.
       •  Characterize the relative
         toxicities of coarse, fine, and
         ultrafme PM from several
         different geographical areas
         that have different sources of
         PM pollution. The chemical
         composition of these size
                ranges can simultaneously be
                associated with the health
                outcomes.
              • Characterize the relative
                toxi city of different particle
                sizes from real world sources,
                notably near highly trafficked
                roadways.
              • Conduct epidemiological field
                studies to assess size related
                health impacts on selected
                populations of high risk.
                especially near roadways.

              Application  and Impact:
              Research to expand our
              knowledge about the health
              effects associated with exposure
              to different  size particles will
              provide important information
              that will allow EPA to set PM
              standards which are optimally
              protective of human health.

              The research will help EPA
              regulators:

              • Determine if it is appropriate to
                regulate particles based on the
                number of particles rather than
                size.
              • Assess whether ultrafme
                particles should be regulated.
  Assist with making decisions
  on where to position air quality
  monitors to obtain the data that
  best represents human health
  risks.
REFERENCES
Gilmour, MI, et al. Comparative Toxicity of Size
Fractionated Airborne Particulate Matter Obtained
from Different Cities in the USA. Inhalation
Toxicology, 2007; 1:7-16.

Graff, DW, et al. Assessing the Role of Particulate
Matter Size and Composition on Gene Expression
in Pulmonary Cells. Inhalation Toxicology, 2007;
1:23-8.

Samet, JM, et al. A Comparison of Studies on the
Effects of Controlled Exposure to Fine, Coarse and
Ultrafme Ambient Particulate Matter from a Single
Location. Inhalation Toxicology, 2007; 1:29-32.

Frampton M.W., et al. Inhalation of Ultrafine
Particles alters Blood Leukocyte Expression of
Adhesion Molecules in Humans. Environmental
Health Perspectives, 2006; 114(1): 51-58.

Peng R.D., et al. Coarse particulate matter air
pollution and hospital admissions for
cardiovascular and respiratory diseases among
Medicare patients. Journal of the American
Medical Association, 2008; 299(18): 2172-9.
CONTACTS
Robert Devlin, Ph.D., National Health and
Environmental Effects Research Laboratory,
EPA's Office of Research and Development, 919-
966-6255, devlin.robert@epa.gov

M. Ian Gilmour, Ph.D., National Health and
Environmental Effects Research Laboratory, 919-
541-0015, gilmour.ian@epa.gov

JANUARY 2009
       U.S. Environmental Protection Agency
       Office of Research and Development

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