United States       Prevention, Pesticides      EPA712-C-08-009
         Environmental Protection    And Toxic Substances       October 2008
         Agency        (7101)
&EPA   Fate, Transport and
         Transformation Test
         Guidelines
         OPPTS 835.1230
         Adsorption/Desorption
         (Batch Equilibrium)

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                            INTRODUCTION
      This guideline is one of a series of test guidelines that have been
developed by the Office of Prevention, Pesticides and Toxic Substances
(OPPTS), United States Environmental Protection Agency for use in the testing
of pesticides and toxic substances, and the development of test data to meet the
data requirements of the Agency under the Toxic Substances Control Act (TSCA)
(15 U.S.C. 2601), the Federal Insecticide, Fungicide and Rodenticide Act
(FIFRA) (7 U.S.C. 136, et seq.), and section 408 of the Federal Food, Drug and
Cosmetic (FFDCA) (21 U.S.C. 346a).

      OPPTS developed this guideline through a process of harmonization of
the testing guidance and requirements that existed for the Office of Pollution
Prevention and Toxics (OPPT) in Title 40, Chapter I, Subchapter R of the Code
of Federal Regulations (CFR), the Office of Pesticide Programs (OPP) in
publications of the National Technical Information Service (NTIS) and in the
guidelines published  by the Organization for Economic Cooperation and
Development (OECD).

      For additional  information about OPPTS harmonized guidelines and to
access this and other guidelines, please go to http://www.epa.gov/oppts and
select "Test Methods & Guidelines" on the  left side menu.

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OPPTS 835.1230 Adsorption/desorption (batch equilibrium)
       (a)  Scope—(1) Applicability.   This guideline is intended for use in  meeting testing
requirements of the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) (7 U.S.C. 136,
et seq.) and for testing pursuant to the Toxic Substances Control Act (TSCA) (15 U.S.C. 2601, et
seq.)  It describes procedures that, if followed, would result in data that would generally be of
scientific merit for the purposes described in paragraph (b) of this guideline.

       (2)  Background. The source materials used in developing this harmonized OPPTS test
guideline are OPP  163-1 Leaching and adsorption/desorption,  EPA report 540/9-82-021, CFR
796.2750 Sediment and soil adsorption isotherm, OECD Guideline for Testing of Chemicals 106
Adsorption-Desorption Using a Batch Equilibrium Method (adopted January 2000), and OPPTS
5.1220 Sediment and Soil Adsorption/Desorption Isotherm.

       (b)    Purpose   (l)Adsorption/desorption   studies    are   used    to   estimate    the
adsorption/desorption behavior of a substance on soils. The goal is to obtain a  sorption value which
can be used to predict partitioning  under a variety of environmental conditions; to this end,
equilibrium adsorption coefficients for a chemical on various soils are determined as a function of
soil characteristics (e.g., organic carbon content, clay content,  soil texture, and pH). Different soil
types have  to be used in order to cover as widely as possible the interactions  of a given substance
with naturally occurring soils.

       (2)  Adsorption/desorption  studies generate  essential information  on  the mobility  of
chemicals and their distribution in the soil, water and  air compartments of our  biosphere,  (see
paragraphs  (j)(l)  through (j)(10) °f this  guideline).   They  can  be used  in the prediction  or
estimation,  for example, of the  availability of a chemical  for degradation (see paragraphs 0)01)
and 0)(12)  of this  guideline), transformation and uptake by organisms (see paragraph (j)(13) of this
guideline; leaching through the  soil profile (see paragraphs 0)5), (j)(7), 0)09, 0)(10) and 0)04)
through 0)07)  of this guideline), volatility from soil (see paragraphs 0)(10), 0)18) and 0)19) of
this guideline); and run-off from land surfaces into natural waters (see paragraphs 0)(7), 0)(20) and
(j)(21) of this guideline) as well as for comparative and modeling purposes (see paragraphs 0)09
and 0)(22) through 0)(24) of this guideline)..  The distribution of a chemical between soil and
aqueous phases is a complex process depending on a number of different factors: the chemical
nature  of the substance (see paragraphs 0)(1)  and 0)(25) through 0)(29) of this guideline),  the
characteristics of the soil (see paragraphs 0)(30), 0)(1) through (0)(3) and 0)(31) through 0)(39) of
this guideline), and climatic factors such as rainfall, temperature, sunlight and wind.

       (c) Definitions and symbols—(1) Definitions

       Adsorption ratio, Kd is the amount of test chemical  adsorbed by a sediment or soil (i.e., the
solid phase) divided by the amount of test chemical in the solution phase, which is in equilibrium
with the solid phase, at a fixed solid/solution ratio.

       Cation exchange capacity (CEC) is the sum total of exchangeable cations that a sediment
or soil can  absorb.  The CEC is expressed in milliequivalents of negative charge per 100 grams
(meq/lOOg) or milliequivalents of negative charge per gram of soil or sediment.

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       Clay mineral analysis is the estimation or determination of the kinds of clay-size minerals
and the amount present in a sediment or soil.

       Organic matter is the organic fraction of the sediment or soil; it includes plant and animal
residues  at various stages of decomposition, cells and tissues of soil organisms, and  substances
synthesized by the microbial population.

       Particle size analysis is the determination of the various amounts of the different particle
sizes  in  a sample (i.e., sand,  silt, clay),  usually  by sedimentation,  sieving,  micrometry, or
combinations of these methods.  The  names and diameter range  commonly used  in the United
States are shown in Table 1 following.

          Table 1. Particle size distribution names and ranges used in the United States.
Name
Very coarse sand
Coarse sand
Medium sand
Fine sand
Very fine sand
Silt
Clay
Diameter range
2.0 to 1.0mm
1.0 to 0.5 mm
0.5 to 0.25 mm
0.25 to 0.125 mm
0.125 to 0.05 mm
0.05 to 0.002 mm
< 0.002 mm
       pH of a sediment or soil is the negative logarithm to the base ten of the hydrogen ion
activity of the sediment or soil  suspension.  It is usually measured by a suitable sensing electrode
coupled with a suitable reference electrode at a 1/1 solid/solution ratio by weight.

       Sediment is the unconsolidated  inorganic and  organic  material that is suspended in and
being transported by surface water, or has settled out and has deposited into beds.

       Soil is the unconsolidated mineral material on the immediate surface of the earth that serves
as a natural medium for the  growth of land plants.  Its formation and properties are determined by
various factors such as parent material, climate, macro-  and microorganisms, topography, and time.

       Soil aggregate is the combination or arrangement of soil separates (sand, silt, clay) into
secondary units.  These units may be arranged in the soil profile  in a distinctive characteristic
pattern that can be classified according to size, shape, and degree of distinctness into classes, types,
and grades.

       Soil classification is  the systematic arrangement of soils into groups or categories.  Broad
groupings are based on general soil characteristics while subdivisions are based on more detailed
differences in specific properties.  The soil classification system used in this guideline and the one
used today in the United States is the 7th Approximation-Comprehensive System. The ranking of
subdivisions under this system is: Order, Suborder, Great group, Family, and Series.

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       Soil horizon is a layer of soil approximately parallel to the land surface. Adjacent layers
differ in physical, chemical, and biological properties such as color, structure, texture, consistency,
kinds and numbers  of organisms present, and degree of acidity or alkalinity.

       Soil Order  is the  broadest category of soil classification and is based on the general
similarities of soil physical/chemical properties.  The formation of soil  by similar general genetic
processes causes these similarities.  The Soil Order found in the United  States are:  Alfisol,
Aridisol, Entisol, Histosol, Inceptisol, Mollisol, Oxisol, Spodosol, Ultisol, and Vertisol.

       Soil series is the basic unit of soil classification and is  a subdivision of a family. A series
consists of soils that were  developed under comparable climatic and vegetational conditions.  The
soils comprising a  series  are essentially alike in all major profile characteristics except  for the
texture of the "A" horizon  (i.e., the surface layer of soil).

       Soil texture  is a classification of soils that is based on the relative proportions of the various
soil separates present. The soil textural classes are:  clay, sandy clay,  silty clay, clay loam,  silty
clay loam, sandy clay loam, loam, silt loam, silt, sandy loam, loamy sand, and sand.

       (2) Symbols for equations. The weight of soil sample in the equations of this guideline refer
to the oven dry weight.

                     Table 2. Symbols for equations used in this guideline
Symbol
At,
Aeq
mfa.)
mf(Atl)
mf(eq)
mo
m?(ti)
ads / \
maq (eq)
nw
cst
C0
Oo
Definition
adsorption percentage at the time point tj
adsorption percentage at adsorption equilibrium
Mass of the test substance adsorbed on the soil at the time ti
mass of the test substance adsorbed on the soil during the time
interval At;
mass of the substance adsorbed on the soil at adsorption
equilibrium
mass of the test substance in the test tube, at the beginning of
the adsorption test
mass of the substance measured in an aliquot ( v A ) at the time
point ti
mass of the substance in the solution at adsorption
equilibrium
quantity of the soil phase, expressed in dry mass of soil
Mass concentration of the stock solution of the substance
initial mass concentration of the test solution in contact with the
soil
mass concentration of the substance in the aqueous phase at the
time ti that the analysis is performed
Units
%
%
Hg
M«
Hg
Hg
M«
M«
g
|ag cm"3
lag cm'3
ug cm"3

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Symbol

Cf(eq)
C?(eq)
V0
A
va
Kd
Koc
Kom
Kads
F
1/n
Dt,
°At,
Kdes
Kdes
F
mJCti)
m£(Ati)
mf(eq)
mj(eq)
mf(Atl)
<
CfCeq)
Ca>q)
.VT
VR
Definition

content of the test substance adsorbed on soil at adsorption
equilibrium
mass concentration of the substance in the aqueous phase at
adsorption equilibrium
initial volume of the aqueous phase in contact with the soil
during the adsorption test
volume of the aliquot in which the test substance is measured
distribution coefficient for adsorption
organic carbon normalized adsorption coefficient
organic matter normalized distribution coefficient
Freundlich adsorption coefficient
Freundlich exponent
desorption percentage at a point time ti
desorption percentage corresponding to a time interval At;
apparent desorption coefficient
Freundlich desorption coefficient
Mass of the test substance desorbed from soil at the time ti
mass of the test substance desorbed from soil during the time
Ati
mass of substance determined analytically in the aqueous phase
at desorption equilibrium
Total mass of test substance desorbed at desorption equilibrium
mass of the substance remaining adsorbed on the soil after the
time interval A ti
mass of the test substance left over from the adsorption
equilibrium due to incomplete volume replacement
content of the test substance remaining adsorbed on the soil at
desorption equilibrium
mass concentration of the substance in the aqueous phase at
desorption equilibrium
total volume of the aqueous phase in contact with the soil
during the desorption kinetics experiment performed with the
serial method
volume of the supernatant removed from the tube after the
attainement of adsorption equilibrium and replaced by the same
volume of a 0.01 M CaC12 solution
Units

ugg1
lag cm'3
cm3
cm3
cm3 g"1
cm3 g1
cm3 g"1
^(cnrYV

%
%
cm3 g"1
Mg^cmW1
ug
ug
ug
ug
M«
Hg
ugg"1
ug cm"3
cm3
cm3

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Symbol
D
va
v;
vrF
MB
mE
vrec
-*- ow
pKa
sw
Definition
volume of the aliquot sampled for analytical purpose from the
tube (i), during the desorption kinetics experiment performed
with the serial method
volume of the solution taken from the tube (i) for the
measurement of the test substance, in desorption kinetics
experiment (parallel method)
volume of the solution taken from the tube for the measurement
of the test substance, at desorption equilibrium
Mass balance
total mass of test substance extracted from the soil and walls of
the test vessel in two steps
volume of the supernatant recovered after the adsorption
equilibrium
octanol/water partition coefficient
dissociation constant
water solubility
Units
cm3
cm3
cm3
%
ug
cm3


gr1
       (d) Principle of the test.  (1) In this guideline, adsorption represents the process of the
binding of a chemical to surfaces of soils; it does not distinguish between  different adsorption
processes (physical and chemical adsorption) and such processes as surface catalyzed degradation,
bulk adsorption or chemical reaction. Adsorption that will occur on colloid particles (diameter <
0.2 |j,m) generated by the soils is not fully taken into account.

       (2) Known volumes  of solutions of the test substance,  non-labeled or radiolabeled,  at
known concentrations in 0.01 M CaCb are added to soil samples of known dry weight which have
been pre-equilibrated in 0.01  M CaCl2.  The mixture is agitated for an appropriate time. The soil
suspensions  are  then  separated by centrifugation,  which  may also  include  filtration,  with
subsequent analysis of the aqueous  phase.  The amount  of test substance adsorbed on the soil
sample is calculated as the difference between  the amount of test substance initially  present in
solution and the amount remaining at the end of the experiment (indirect method).

       (3) The amount of test substance adsorbed  can also be directly determined by analysis of
soil. Although this makes the analytical procedure more tedious, involving stepwise soil extraction
with an  appropriate  solvent, it is recommended in cases where the difference  in the  solution
concentration of the substance cannot be  accurately determined. Examples of such  cases are:
adsorption of the test substance on surfaces of the  test vessels; instability of the test substance in
the time  scale of the experiment; weak adsorption giving  only small concentration  change in the
solution; and strong adsorption yielding low concentration  which cannot be accurately determined.
If a radiolabeled substance is  used, soil extraction may be avoided by analysis of the soil phase by
combustion  and  liquid  scintillation counting.    However,  liquid  scintillation  counting  is  a
nonspecific technique which  cannot differentiate between the test chemical and  its transformation
products; therefore it should be used only if the test  chemical is  stable for the duration of the study.

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       (4) Testing is designed to evaluate the adsorption of a chemical on different soil types with
a varying range of organic carbon content, clay content and soil texture, and pH. Methods for the
preliminary  study and the main study are generally the same, exceptions are mentioned  where
relevant. It comprises three tiers.  The tiers are as follows:

       (i) Preliminary study.  In order to determine the soil/solution ratio, the equilibration time
for adsorption and the amount of test substance adsorbed at equilibrium and the adsorption of the
test substance on the surfaces of the test vessels and the stability of the test substance during the
test period.
       (ii) Screening test.  The adsorption is studied in five different soil types by means of
adsorption kinetics at a single concentration and determination of distribution  coefficients for
adsorption (Kj) and organic carbon normalized adsorption distribution coefficient (Koc).

       (iii)  Main study:  Determination of Freundlich adsorption isotherms to determine the
influence of concentration on the extent of adsorption on soils. Study of desorption by means of
desorption kinetics/Freundlich desorption isotherms is shown in the following Figure 1.

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Fig. 1.—Testing scheme.
                      Set up of a sui|a.ble analytical
                                     T
                                  Available?
                                    \Ye;
                                     \y
                                      1
                                                         No further test
                                Preliminary
Selection of
optimal
soil/solution ratios

Determination of
equilibrium time

Check for
adsorption on the
vessel surface

Check of the stability
by means of parental
mass balance
                       Equilibrium plateau is achieved?
                                       J
                               Adsorption kinetic.?
                                                              If mass balance < 90 %, test
                                                              substance is unstable in the time
                                                              scale of the test. The test can
                                                              continue by analyzing both phases
                                                              (soil and aqueous solution).
rs

P
                                     5 soils
                     (indirect method: measurement of solution depletion)
                                          or
                     (direct method: measurement of both phases)
                                             as appropriate
    CO
                       isothertns
                                                          1
                                                  Desorption kinetics
                                                      Desorption isotherms

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        (e) General considerations—(1) The soil parameters that are believed most important for
adsorption are: organic carbon content (see paragraphs (j)(30) and (j)(40), (j)(l) through ((j)(3),
(j)(31) and (j)(33) through (j)(38) of this guideline); clay content and soil texture (see paragraphs
(j)(30) and (j)(40), and G)(32) through (j)(38) of this guideline); and pH for ionizable compounds
(see paragraphs (j)(30), (j)(32) and (j)(40) of this guideline). Other soil parameters which may have
an impact on the adsorption/desorption of a particular substance are the effective cation exchange
capacity (ECEC), the content of amorphous  iron and aluminum oxides,  particularly for volcanic
and tropical soils (see paragraph (j)(30) of this guideline), as well as the specific surface  (see
paragraph (j)(39) of this guideline).
        (2) Influence of analytical method. The key parameters that can influence the accuracy of
sorption measurements include the  accuracy of the analytical  method in analysis  of both  the
solution and adsorbed phases, the stability and  purity of the test substance,  the  attainment of
sorption equilibrium, the magnitude of the solution concentration change, the  soil/solution ratio,
and changes in the soil structure during the equilibration process (see Table 3). The reliability of
the analytical method used should be checked at the concentration range which is likely to occur
during the  test.  The  experimenter  should feel  free to develop  an appropriate  method with
appropriate accuracy, precision, reproducibility, detection limits and recovery.

       (3) From the following (see reference in paragraph (j)(41)) it becomes obvious that when
the difference between the initial mass (nio =110 jig) and equilibrium concentration (m*  (eq) =
100 jig) of the test substance in the solution is very small, an error of 5% in the measurement of
equilibrium concentration results in an  error of  50% in the calculation  of the mass of  the
substance adsorbed in soil (mfs(eq)) and of 52.4% in the calculation of the Kd.


                            Amount of soil msoll  = 10 g

                            Volume of solution V0 = 100 cm3

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          Table 3. Influence of accuracy of analytical method and concentration change
on accuracy of adsorption results (m0 = 110 ug or C0 = 1.100 M9/cm




"a
o
~tob
=J.
o
o
,-H'
II
0
U
IH
O
bO
O
II
o
£


mj(eq) Cj(eq) R
(ug) (ug cm"3)
For A = 9%
100 1.000 true value
101 1.010 1%
105 1.050 5%
109 1.090 9%
For A = 55%
50.0 0.500 true value
50.5 0.505 1%
52.5 0.525 5%
55.0 0.550 10%
For A = 99%
1.100 0.011 true value
1.111 0.01111 1%
1.155 0.01155 5%
1.21 0.0121 10%
mfCeq)* Cf(eq) R*
(ug) *(ugg-1)

10 1.00 true value
9 0.90 10%
5 0.50 50%
1 0.10 90%

60.0 6.00 true value
59.5 5.95 0.8%
57.5 5.75 4.0%
55.0 5.50 8.3%
108.9 10.89 true value
108.889 10.88 0.01%
108.845 10.8845 0.05%
108.790 10.8790 0.10%

Kd* R*

1
0.891 10.9%
0.476 52.4%
0.092 90.8%

12.00
11.78 1.8%
10.95 8.8%
10.00 16.7%
990
980 1.0%
942 4.8%
899 9.2%
    mf(eq)
                m0-i(eq),  Cf (eq) =
                                              [C0  -Qeq)]vo
Kd =
                                                                         ads
                                                                           /  \  -* T
                                                                           (eg)  V0
mf(eq)

mj(eq)

Cf(eq)
C?(eq)

R
                    mass of the test substance in the soil phase at equilibrium, ug;
                    mass of the test substance in the aqueous phase at equilibrium, ug;

                    content of the test substance in the soil phase at equilibrium, ug g"1;
                    mass concentration of the test substance in the aqueous phase at equilibrium, ug cm"3.
                    analytical error in the determination of the m
                    calculated error due to the analytical error R.
       (4) The test is applicable to chemical substances for which an analytical method with
sufficient accuracy is available.  An important parameter that can influence the reliability of the
results, especially when the indirect method is followed (see paragraph (d)(2) of this guideline),
is  the stability of the test  substance in the time  scale of the test.   The stability of the test
substance should be determined  in a preliminary study; if a transformation in the time scale of

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the test is observed, the main study should be performed by analyzing both soil and aqueous
phases.

       (5)  Difficulties  may  arise in conducting  this test for test  substances with low  water
solubility (Sw  <10"4 g  I"1), as well  as  for highly charged substances, due to the fact that the
concentration in the aqueous phase cannot be measured analytically with sufficient accuracy.  In
these cases, additional steps have to be taken.  Guidance on  how to deal with these problems is
given in the relevant sections of this Guideline.

       (6) When testing volatile substances, care should be taken to avoid losses during the
study.

       (7)  The following  information  on the  test substance should be available: solubility in
water, vapor pressure and Henry's law  constant; abiotic hydrolysis as a function of pH;  n-
octanol/water  partition  coefficient;  ready  biodegradability   or  aerobic  and   anaerobic
transformation  in soil; pKa of ionizable substances; and direct photolysis in water (i.e., UV-Vis
absorption spectrum in water, quantum yield) and photodegradation on soil.

        (f)  Test method—(1) Test substance. The test substance may  be radiolabeled with
known composition and radiopurity or, if unlabelled substance is used, it  should be of known
composition and at least 95%  purity.

        (2) Test conditions,   (i) All experiments are done at  laboratory ambient temperature and,
if possible, at a  constant temperature between 20 °C and 25 °C.

        (ii) Centrifugation conditions should allow the removal  of particles larger than 0.2 |j,m
from  the solution.  This value triggers the  smallest  sized particle that is considered as a  solid
particle, and is the limit between solid and colloid particles. Correct centrifugation conditions can
be determined by  referring to the equipment manufacturer's instructions.   If the centrifugation
facilities cannot  ensure that particles  larger  than  0.2  |j,m are removed,  a combination  of
centrifugation and filtration with 0.2 |j,m filters could  be used.  These filters should be made of a
suitable inert material to avoid any losses of the test substance on them. In any case, it should be
demonstrated that no losses of the test substance occur during filtration.

        (3) Soils—(i) The soils should be characterized by  parameters considered to be largely
responsible for the adsorptive capacity: organic carbon, clay content, pH,  textural composition
(percent sand, silt,  and clay), and textural class.  Other physico-chemical properties of the soil may
have an impact on the adsorption/desorption of a particular substance and should be considered in
such cases.

        (ii) The methods used for  soil  characterization  are very  important and can have  a
significant influence on the results.  Soil pH should be measured in a solution of 0.01 M CaCl2
(that is the  solution used in  adsorption/desorption testing) according  to the corresponding ISO
method (as referenced in (j)(42) of this guideline).The other relevant soil properties should also be
determined according to standard methods to permit the  analyses of sorption data to be based on
globally  standardized soil  parameters.   Some guidance for  existing  standard  methods of soil
                                            10

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analysis and characterization is  given  in references G)(43) through  (j)(45) of this  guideline,  or
calibration of soil test methods, the use of reference soils may be considered.

       (iii)  Studies should be conducted with  soils that are  representative of agricultural areas
where the  pesticide  will  be used.  Soils  should be  characterized according  to the USDA
classification system. At least one of the soils selected should have an organic matter content less
than or equal to one percent (sand or sandy loam preferred).

       (iv)  Soil from foreign sources may be used, providing the foreign soil will have the same
characteristics  as soil in the United  States common to the proposed  use area.  Additional
information  on use  of foreign soils may be  obtained from  the document  "Guidance  for
Determining the Acceptability of Environmental Fate Studies Conducted with Foreign Soils," at
the U.S. Environmental Protection Agency's Environmental Fate and Effects Division, Office of
Pesticides (see paragraph (j)(46). If the pesticide is intended  for an aquatic use or for an aquatic
impact use  involving direct discharges of treated water into outdoor aquatic  sites, an aquatic
sediment obtained from or representative of the proposed use  should be included.

       (4) Soil collection.  The sampling site should be exactly defined by UTM (Universal
Transversal  Mercator-Projection/European Horizontal Datum)  or geographical coordinates in order
to allow re-collection of a particular soil in the future or could help in defining soil under various
classification systems used in different countries.  Only the A horizon up to a maximum depth of
20 cm should be collected.

       (5) Soil transportation.  The  soil  samples should be transported using containers and
under temperature conditions which guarantee that the initial  soil properties are not significantly
altered.

       (6) Soil storage. The use of soils freshly taken from  the  field is preferred.  If this is not
possible, soil can be stored  at ambient temperatures and should be kept air-dried.  No limit on the
storage time is recommended, but soils stored for more than three years should be re-analyzed prior
to the use with respect to their organic carbon content, pH and CEC.

       (7) Soil preparation—(i)  Soils are to be air-dried at ambient temperature (preferably
between 20-25  °C). Disaggregation should be performed with minimal force, so that the original
texture of the soil will be changed as little as possible.  The soils are sieved to a particle size <2
mm;  recommendations  of the ISO  standard  on  soil  sampling  (see paragraph  (j)(42) of this
guideline) should be  followed with  respect to the sieving process.   Careful homogenization is
recommended, as this enhances the reproducibility of the results.

       (ii) Moisture content of each soil is  determined on three aliquots with heating at 105 °C
until there is no significant change in weight (approx. 12h).  For all calculations the mass of soil
refers to oven dry mass,  i.e., the weight of soil corrected for moisture content.

       (8) Test substance preparation, (i) The test substance is dissolved in a 0.01 M solution of
calcium chloride (CaC^) in distilled or de-ionized water; the CaC^ solution is used as the aqueous
solvent phase to improve centrifugation and minimize cation exchange.  The concentration of the
                                            11

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stock solution should preferably be three orders of magnitude higher than the detection limit of the
analytical method used.  The stock solution concentration should be below water solubility of the
test substance.  The stock solution should be prepared just before application to soil  samples and
should be kept closed in the dark at 4 °C.  Storage time  depends on the  stability of the test
substance and its concentration in the solution.

       (ii) For poorly soluble substances (Sw < 10"4 g L"1), an appropriate solubilizing agent should
be used  when it is difficult to dissolve the test substance.  This  solubilizing agent should be
miscible with water, such as methanol or acetonitrile.  Its concentration should not exceed  1% of
the total volume of the stock solution and should constitute less than that in the solution of the test
substance which will come  in contact with the soil (preferably less than 0.1%).  The solubilizing
agent should not be a surfactant or undergo solvolytic reactions with the test chemical

       (iii) Another alternative for poorly soluble substances is to add the test substance to the test
system by spiking: the test substance is dissolved in an organic  solvent, an aliquot of which is
added to the system of soil and 0.01 M solution of CaCl2 in distilled  or de-ionized water.  The
content of organic  solvent in the aqueous phase should be kept as low as possible, normally not
exceeding 0.1%.  However, spiking from an  organic solution may  suffer from volume changes,
leading to an additional error  as  the test substance and co-solvent concentration would not be
exactly the same in all tests.

       (9) Soil/solution ratios, (i) Selection of appropriate soil to solution ratios  for  sorption
studies depends  on the distribution coefficient Kd and the relative degree of adsorption desired.
The  change of substance concentration in the solution determines the  statistical accuracy  of the
measurement based on the form of adsorption equation and the limit of the analytical methodology,
in detecting the concentration of the chemical in  solution.  Therefore, in general practice it is useful
to settle  on  a few fixed  ratios, for which the percentage adsorbed is above 20%, and preferably
>50% (see reference in paragraph (j)(47), while care should be taken to keep the test substance
concentration in the aqueous phase high enough to be measured accurately.  This is particularly
important in the case of high adsorption percentages.

       (ii) A convenient approach to selecting the appropriate soil/water  ratios  is based  on an
estimate of the Kd value  either by  preliminary studies or by established estimation techniques (see
paragraph (h)(l) of this guideline.  Selection of an appropriate ratio can then be made based on a
plot  of soil/solution ratio versus Kd for  fixed percentages of adsorption (see the following Figure
2).
                                             12

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       Figure 2.—Relationship between soil to solution ratios and Kd at various percentages of
adsorbed test substance.
        10
    as
   <*f
    c
   .2
    «•*
   _3
    »M^
   X
_
1*

0,1
                          1
                                          10
100
1000
                        Distribution coefficient K  (cm3
       (A) In this plot it is assumed that the adsorption equation is linear (according to equation 1).
Equation 1
                                  „ ads .  .    -,,   „ ads
                                  Cs  («1)  = Kd 'Caq
       (B) The applicable relationship is obtained by rearranging equation (4) of the
form of equation 2.
                                                                                   in the
Equation 2
                                           mr
                               ni
                                                  -1)KC
or in its logarithmic form assuming that
Equation 3
                                                       ads
                          R = msoii/Vo and Aeq%/100 =
                                                         (eq)
                                           13

-------
then

Equation 4

                                              (Aea%/100)
                                                 q
                       log R = - log Kd + log
                                             (1.Aeqo/o/100)
       (iii) Figure 2 shows soil/solution ratios as a function of Kd for different levels of adsorption.
For example, with a soil/solution ratio of 1:5 and a Kd of 20, approximately 80% adsorption would
occur. To obtain 50% adsorption for the same Kd, a 1:25 ratio should be used.  This approach to
selecting  the appropriate  soil/solution ratios  gives the  investigator  the flexibility  to meet
experimental needs.

       (iv)  Where  low  adsorption of test  substance  occurs,  a  1:1   soil/solution  ratio  is
recommended, although for some very organic soil types smaller ratios may be necessary to obtain
a slurry.  In any case, care should be taken with the analytical methodology to measure small
changes in solution concentration;  otherwise the adsorption measurement will be inaccurate.  On
the other hand, at very high distribution coefficients Kd, one can go up to a 1:100 soil/solution ratio
in order to leave  a significant amount of chemical in solution.  However, care should be taken to
ensure good mixing,  and adequate time should be allowed for the system to equilibrate.  An
alternative approach to deal with these extreme cases when adequate analytical methodology is
missing, is to predict the Kd value  applying estimation  techniques based, for example, on Pow
values (see  paragraph (h)(l)  of  this  guideline).   This  could  be  useful especially  for low
adsorbed/polar chemicals with Pow < 20 and for lipophilic/highly sorptive chemicals with Pow >
104.
       (10) Analytical method,  (i) An appropriate volume of 0.01 M CaCb, e.g.,  100 cm3, is
agitated during 4 h with a weight of soil,  e.g., 20 g, of high adsorbability, i.e. with high organic
carbon and clay content; these weights and volumes may vary depending on analytical needs, but
a soil/solution  ratio of 1:5 is a convenient starting point.  The mixture is centrifuged  and the
aqueous phase may be filtrated.  A certain volume of the test substance stock solution is added to
the latter to reach a nominal concentration within the concentration range which is likely to occur
during the test. This volume should not exceed 10% of the final volume of the aqueous phase, in
order to change as little as possible the nature of the pre-equilibration solution.  The solution is
analyzed.

       (ii)  One blank run consisting of the system soil + CaCb solution (without test substance) is
included, in order to check for artifacts in the analytical method and for matrix effects  caused by
the soil.

       (iii) Analytical methods which can be used  for sorption measurements include  gas-liquid
chromatography (GLC), high-performance liquid  chromatography (HPLC), spectrometry (e.g.,
GC/mass   spectrometry, HPLC/mass  spectrometry)  and liquid  scintillation  counting  (for
radiolabeled substances).  Independent  of the  analytical method used, recoveries should be
between 90% and 110% of the nominal value.  In order to  allow for detection and evaluation  after
                                            14

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partitioning has taken place, the detection limits of the analytical method should be at least two
orders of magnitude below the nominal concentration.

       (iv) The characteristics and detection limits of the analytical method available for carrying
out adsorption studies play  an important role  in defining the test conditions  and the whole
experimental performance of the test. This guideline  provides recommendations and guidance for
alternative solutions where the analytical method and laboratory facilities may impose limitations.

       (g) Test sequence—(1) Preliminary study—Tier 1 (i) Selection of optimal  soil/solution
ratios. (A) Two soil types and three soil/solution ratios (six experiments) are used. One soil type
has high organic carbon  and low clay content, and the other low organic carbon and high clay
content.  The following soil to  solution ratios are suggested. However, the absolute  mass of soil
and volume of aqueous  solution corresponding  to these ratios can  be different with respect to
laboratory facilities:
       (1) 50 g soil and 50 cm3 aqueous solution of the test substance (ratio 1/1).
       (2) 10 g soil and 50 cm3 aqueous solution of the test substance (ratio 1/5).
       (3) 2 g soil and 50 cm3 aqueous solution of the test substance (ratio 1/25).

       (B) The minimum amount of soil on which the experiment can be carried out depends on
the laboratory facilities  and the  performance  of  analytical  method used.   However, it  is
recommended to use at least 1 g, and preferably 2 g, in order to obtain reliable results from the test.

       (C) One control sample with only the test substance in 0.01 M CaCb solution (no soil) is
subjected to precisely the same steps as the test systems, in order to check the stability of the test
substance in CaCb solution and its possible adsorption on the surfaces  of the test vessels.

       (D) A blank run per soil with the same amount of soil and total volume of 50 cm3 0.01 M
CaCb solution (without test substance) is  subjected to the same test  procedure.  This serves as a
background control during the analysis to detect interfering compounds or contaminated soils.

       (E) All  experiments, including  controls  and blanks, should be performed at  least  in
duplicate.  The  total number of the  samples which should be prepared for the study can be
calculated with respect to the methodology which will be followed (see paragraph (g)(l)(i)(J) of
this guideline).
   3
       (F) The air-dried soil samples are equilibrated by shaking with a minimum volume of 45
cnr* of 0.01 M CaCb overnight (12 h) before the day of the experiment.  Afterwards, a  certain
volume of the stock solution of the test substance is added in order to adjust the final volume to 50
cm3.  This volume of the stock  solution added:  (a) should not exceed 10%  of the final  50 cm3
volume of the  aqueous phase in order to  change as little as possible the nature of the pre-
equilibration solution;   and (b) should preferably  result  in an  initial concentration of the test
substance being in contact with the soil (Co) at least two orders of magnitude higher than the
detection limit of the analytical method. This threshold safeguards the ability  to perform accurate
measurements even when strong adsorption occurs (> 90%) and to determine later the adsorption
isotherms.  It is also recommended, if possible,  that the initial  substance  concentration (Co) not
exceed half of its solubility limit.
                                            15

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       (G) An example of how to calculate the concentration of the stock solution (Cst) is as
follows:  a detection limit of 0.01 |j,g cm"3 and  90% adsorption  are assumed;  thus,  the initial
concentration of the test substance in contact with the soil should preferably be 1 |j,g cm"3 (two
orders of magnitude higher than the detection limit).  Supposing that the maximum recommended
volume of the stock solution is added, i.e. 5 to 45 cm3 0.01 M CaCb equilibration solution (= 10%
of the stock solution to  50  cm3 total volume  of aqueous phase),  the concentration of the stock
solution should be 10 |j,g cm"3;  this is three orders of magnitude higher than the detection limit of
the analytical method.

       (H) The pH of the aqueous phase should be measured before and after contact with the  soil
since it  plays  an  important  role in the whole adsorption process,  especially  for ionizable
substances.

       (I) The mixture is shaken until adsorption equilibrium is reached.  The equilibrium time in
soils is highly variable, depending on the chemical  and the  soil;  a period of 24 h is generally
sufficient (see paragraph (j)(48) in this  guideline).  .In the preliminary  study, samples may be
collected sequentially over a 48 h period of mixing (for example at 4, 8, 24, 48 h). However, times
of analysis should be considered with flexibility of the work schedule of the laboratory.

       (J) There are two options for the analysis of the test substance in the aqueous solution:  the
parallel  method and the serial method.   Although the parallel method is experimentally more
tedious,  the  mathematical treatment of  the  results is simpler (see paragraph  (h)(2) of  this
guideline).

       (1) Parallel method.  Sufficient samples with the same soil/solution ratio are prepared to
accommodate designated time intervals to study the adsorption kinetics.  After centrifugation or
filtration, the aqueous phase of the first tube is recovered as completely as possible and is measured
after, for example, 4 h, that of the second tube after 8 h, that of the third after 24 h, etc.

       (2) Serial method. A duplicate sample is prepared for each soil/solution ratio. At defined
time intervals the mixture is centrifuged  to separate  the phases. A small aliquot of the aqueous
phase is  immediately analyzed for the test substance; then the experiment continues with  the
original mixture. If filtration is applied after centrifugation, the laboratory should have facilities to
handle filtration of small aqueous aliquots. It is recommended that the total volume of the aliquots
taken not exceed 1% of the total volume of the solution, in order  not to change significantly the
soil/solution ratio or decrease the mass of solute available for adsorption during the test.

       (K) The percentage adsorption At. is calculated at each time point (t;) on the basis of the
nominal  initial concentration and the measured concentration at the sampling time (t;), corrected
for the value of the blank.  Plots of the At. versus time (see Fig. 4 of paragraph (h)(2)(ii)(A)(3j
of this guideline) are generated in order to estimate the achievement of equilibrium plateau. Plots
of the concentration of the test substance in the aqueous phase (C^s) versus time could also be
used  to  estimate  the  achievement of  the  equilibrium plateau  (see  Fig.  5  of paragraph
(h)(2)(ii)(A)(3j of this guideline). The Kd value at equilibrium is  also calculated.  Based  on  this
                                             16

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Kd value, appropriate soil/solution ratios are selected from Fig. 4 in paragraph (h)(2)(ii)(A)(3y) of
this guideline, so that  percent adsorption is greater than 20% and preferably greater than 50%
(see reference in paragraph (j)(49) of this guideline).  All applicable equations and principles of
plotting are given in paragraphs (h)(l-7 inclusive) of this guideline).

       (ii)  Determination of adsorption equilibration time and of the amount of test substance
adsorbed at equilibrium. (A) As mentioned in paragraph (g)(l)(i)(K) of this guideline, plots of At.
or C**  versus time permit estimation of the achievement of the adsorption equilibrium  and the
amount of test substance adsorbed at equilibrium.  Figures 4 and 5  in paragraph (h)(2) of this
guideline show examples of such plots.  Equilibration time is the time the system needs to reach  a
plateau.

       (B) If, with a particular soil, no plateau but a steady increase is found, this may be due to
complicating factors such as biodegradation or slow diffusion. Biodegradation can be shown by
repeating the experiment with a sterilized sample of the soil.  If no plateau is achieved even in this
case, the experimenter should search for other phenomena that could  be involved. This could be
done with appropriate modifications of the experimental conditions (temperature, shaking times,
soil/solution ratios).  The experimenter may decide whether to continue the test procedure in spite
of a possible failure to achieve equilibrium.

       (iii) Adsorption  on the surface of the test vessel and stability of the test substance. (A)
Some information on the adsorption of the test substance on the surface of test vessels, as well as
its stability, can be derived by analyzing the control samples.  If a depletion exceeding the standard
error of the analytical method is observed, abiotic degradation and/or adsorption on the surface of
the test vessel could be involved. Distinction between these two phenomena could be achieved by
thoroughly washing the walls of the vessel with a known volume  of  an  appropriate  solvent and
subjecting the wash solution to analysis  for the test substance.  If no adsorption on the surface of
the test vessels is observed, the depletion demonstrates abiotic unstability of the test substance. If
adsorption  is found, changing  the material of the test vessels is necessary. However, data on the
adsorption  on the surface of the  test vessels gained from this experiment cannot be  directly
extrapolated to soil/solution experiment.  The presence of soil will generally reduce this  adsorption.

       (B) Additional information on  the  stability of the test  substance can be  derived  by
determination of the parental  mass balance  over time.  This means that the aqueous phase and
extracts of the soil and test vessel walls are analyzed for the test substance.  The difference
between the  mass of the test chemical added and the  sum of the test chemical masses in the
aqueous phase  and extracts  of the soil and  test  vessel walls is equal to the mass degraded and
volatilized  and  not extracted.  In order to perform a mass balance determination, the adsorption
equilibrium should have been reached within the time period of the experiment.

       (C) The mass balance is conducted on both soils and for one soil/solution ratio per soil that
gives  a  depletion greater than 20% and preferably  greater than 50% at equilibrium.  When the
ratio-finding  experiment is completed with the analysis of the last sample of the aqueous phase
after 48 h,  the phases are separated by centrifugation and,  if so wished, filtration.  The aqueous
phase is recovered as much as possible, and a suitable extraction solvent (extraction coefficient of
                                            17

-------
at least 95%) is added to the soil to extract the test substance.  At least two successive extractions
are recommended.  The amount of test substance in the soil and test vessel extracts is determined
and the mass balance is calculated ( see equation  10, Data and Reporting section in paragraph
(h)(2)(ii)(B)(7) of this guideline).   If it is less than 90%,  the test substance is  considered to be
unstable in the time scale of the test. However, studies could still be continued, taking into account
the instability of the test substance. In this case, both phases in the main study should be analyzed.

       (2) Adsorption  kinetics at one concentration. Tier 2  (i) Five soils are used using the
guidance given in Table 1. There is an advantage to including some or all of the soils used in the
preliminary study among these five soils. In this case, Tier 2 does not have to be  repeated for soils
used in the preliminary  study.

       (ii) The equilibration time, the soil/solution ratio, the weight of the soil sample, the volume
of the aqueous phase in contact with the soil and the concentration of the test substance in the
solution are chosen based on preliminary study results. Analysis should be initiated after about
2, 4,  6, 8  (possibly also  10) and  24 h contact  time;  the agitation time may be extended to a
maximum  of 48  h in case a  chemical takes longer to equilibrate with respect to  ratio-finding
results; however, times of analysis could be considered with flexibility.

       (iii) Each experiment (one  soil  and one  solution)  is done at least in duplicate to allow
estimation of the  variance of the results.  In every experiment  one blank is run.  It consists of the
soil and 0.01 M CaC^  solution, without test substance, and of weight and volume,  respectively,
identical to those  of the  experiment. A control sample with only the test substance in 0.01 M CaCb
solution (without soil) is  subjected to the same test procedure, serving to safeguard against the
unexpected.  The test runs are described in paragraphs (g)(l)(i)(F)  through (g)(l)(i)(K) of this
guideline.

       (iv) The percentage adsorption is calculated at each time point At and/or time interval
AAt (according to the needs of the study) and is plotted over time. The distribution coefficient Kd
at equilibrium, as well as  the organic carbon normalized adsorption coefficient Koc (for non-polar
organic chemicals), are also calculated.

       (v) Results and discussion of the adsorption kinetics test.

       (A) The linear Kd value is generally accurate to describe sorptive  behavior in soil (see
paragraphs (j)(24) and (j)(50) of this guideline) and represents an expression of inherent mobility
of chemicals in soil. For  example, in general chemicals with Ki < 1 cm3 g"1 are considered to be
qualitatively mobile.  Similarly, a mobility classification scheme based on Koc values has been
developed by McCall et al. (j)(5).  Additionally, leaching classification schemes exist based on a
relationship between Koc  and  the  degradation time  for 50% of the test substance (DT-50) (see
paragraphs (j)(20) and (j)(21) of this guideline).

       (B) According to  error analysis studies referenced in (j)(49), Kd values below 0.3 cm3 g"1
cannot be estimated  accurately from a decrease in concentration in the aqueous phase, even when
the most favorable (from point of view of accuracy) soil/solution ratio is applied, i.e. 1:1.  In this
case analysis of both phases, soil and solution, is recommended.
                                             18

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       (C) With respect to the above remarks, it is recommended that the study of  adsorptive
behavior of a chemical in soil and its potential mobility be continued by determining Freundlich
adsorption isotherms for these systems, for which an accurate determination of Kd is possible with
the experimental protocol followed in this guideline.

       (3) Adsorption isotherms and desorption  kinetics/desorption isotherms — Tier 3.  (i)
Adsorption isotherms. (A) Five test substance concentrations  are used, covering preferably two
orders of magnitude;  in the choice of these concentrations the water solubility and the resulting
aqueous equilibrium concentrations should be taken  into account. The same soil/solution ratio per
soil should be  kept along the study.  With one exception, the adsorption  test is performed as
described in paragraphs (g)(l)(i)(B) through  (g)(l)(i)(J) of this guideline. The exception is  the
aqueous phase is analyzed only once at the time necessary to reach equilibrium as determined in
Tier 2. The equilibrium concentrations in the solution are  determined and the amount adsorbed is
calculated from the depletion of the test substance in the solution or with the direct method.  The
adsorbed mass per unit mass of soil is plotted as a function of the equilibrium concentration of the
test substance.

       (B) Results  from the adsorption  isotherms  experiment.    Among  the mathematical
adsorption models proposed so far, the Freundlich  isotherm is the one most frequently used to
describe adsorption processes. More detailed information  on the interpretation and importance of
adsorption models  is provided in references  (j)(31), (J)(35),  and (j)(51) through (j)(53)of this
guideline). It should be mentioned that  a comparison of KF (Freundlich adsorption coefficient)
values for different substances is only possible if these KF values are expressed in the same units
       (ii) Desorption kinetics. (A) The purpose of this experiment is to determine if a chemical is
reversibly or irreversibly adsorbed on a soil.  This information is important, since the desorption
process is key in the behavior of a chemical in field soil.  Moreover, desorption data are useful
inputs in the computer modeling of leaching and dissolved run-off simulation.   If a desorption
study is desired, it is recommended that the study described below be conducted  on  each system
for which an accurate determination of Kd in the preceding adsorption kinetics experiment was
possible.

       (B) As with the adsorption kinetics study, there are two options for the desorption kinetics
experiment: the parallel method and the serial method. The choice of the methodology is left to the
experimenter who should consider the available laboratory facilities and recourses, and remarks
made in paragraph (g)(l)(i)(J) of this guideline.

       (1) Parallel method:  For each soil chosen  for the desorption study,  sufficient samples with
the same  soil/solution ratio are  prepared, to accommodate the   time intervals desired to  study
desorption kinetics. Preferably, the same time intervals as in the adsorption kinetics experiment
should be used;  however, the total time may be extended as appropriate in order for the system to
reach desorption equilibrium.  In every experiment (one soil, one solution) one blank is run.  It
consists of the soil and 0.01  M CaCb solution, without test substance,  and of weight and volume,
respectively, identical to those of the experiment. As a control sample the test substance in 0.01 M
                                            19

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CaCb solution (without soil) is subjected to the same test procedure. Each mixture of soil with
solution is agitated until adsorption equilibrium is reached (as determined in Tier 2).  Then, the
phases are separated by centrifugation and the aqueous phases removed as much as possible. The
volume of solution removed  is replaced by  an  equal volume of 0.01 M  CaCl2  without test
substance and the new mixtures are agitated again.  The  aqueous phase of the  first tube  is
recovered as completely as possible and is measured after, for example, 2 h, that of the second tube
after 4 h, that of the third after 6 h, etc until the desorption equilibrium is  reached.

       (2) Serial method:  After the adsorption kinetics experiment, the mixture is centrifuged and
the aqueous phase is removed as much as possible. The volume of solution removed is replaced by
an equal volume of 0.01 M CaCb without test substance. The new mixture is agitated until the
desorption equilibrium is reached. During this time period, at defined time intervals, the mixture is
centrifuged to separate the phases.  A small aliquot of the aqueous phase is immediately analyzed
for the test substance;  then, the experiment continues with the original mixture. The  volume of
each individual aliquot should be less than 1% of the total volume.  The same quantity of fresh
0.01  M CaCl2 solution is added to the mixture  to maintain the soil to solution ratio, and the
agitation continues until the next time interval.

       (C) The  percentage desorption is calculated at  each  time point Dt   and/or time interval
DAt  (according to the needs of the study) and is plotted over time. The desorption  coefficient
Kdes at equilibrium is also calculated. All applicable equations are given in sections (h)(3) through
(h)(8) inclusive.

       (D) Results from desorption kinetics experiment.  Common  plots  of the percentage
desorption Dt and adsorption  At   versus time,  allow  estimation of the reversibility of the
adsorption process. If the desorption equilibrium is attained even within twice the time of the
adsorption equilibrium,  and  the total desorption is more than 75% of the amount adsorbed , the
adsorption is considered to be reversible.

       (E) Desorption isotherms. Freundlich desorption isotherms are determined on the soils used
in the  adsorption isotherms experiment.  The desorption test is performed as described in the
section "Desorption kinetics" (see paragraphs (g)(3)(ii)(B)(7j or (g)(3)(ii)(B)(%) of this guideline)
with the  only difference  being that the aqueous  phase is analyzed but  once, at  desorption
equilibrium.  The amount of the test  substance desorbed  is  calculated.  The content of test
substance remaining adsorbed on soil  at desorption equilibrium is plotted as a function of the
equilibrium concentration of the test substance in solution

       (h) Considerations  for data  reporting—(1) Estimation  techniques  for  Ka.  (i)
Estimation techniques permit prediction of  Kd based on correlations with, for example, Pow
values (see paragraphs   (j)(l)> G)(28)  and,  (j)(55)  through (j)(60)  in this guideline),  water
solubility data (see paragraphs ((j)(l), (j)(10),G)(8), G)(28), G)(33) and (j)(60) through (j)(64) of
this  guideline),  or polarity data derived  by application of HPLC on reversed  phase ( see
paragraphs (j)(65) through  (j)(67)  of this guideline).  (  See also  Draft OECD Test Guideline
"Estimation  of  Adsorption Coefficient (Koc)  on  Soil and  on  Sewage  Sludge  using  High
Performance  Liquid Chromatography (HPLC)" ,  July  1997 (j)(68)).  As shown in Table 4 and
                                            20

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Table 5 in paragraph (h)(l)(iii) of this guideline, is the K0
equations and then, indirectly, the Kdfrom the equations:
                                                          or Kom that are calculated from these
Equation 5
                                 Koc    Kd
                                              100
                                              %oc
(cm3 g1)
and
Equation 6
                                         Kd
                                                100        3  -K
                               "um     1.724  '  to   (Cm § }

       (ii) The concept of these correlations is based on two assumptions: It is the organic matter
of the soil that mainly influences the adsorption of a substance and the interactions involved are
mainly non-polar. As a result, these correlations are not, or are only to some extent, applicable to
polar substances, and are not applicable in cases where the organic matter content of the soil is
very small (j)(l)- In addition, although satisfactory correlations have been found between Pow and
adsorption (j)(8), the same cannot be said  for the relationship between water solubility and extent
of adsorption (j)(8) and (j)(10);  so far the studies are  contradictory.

       (iii) Some examples of correlations between the adsorption coefficient and the octanol-
water partition coefficient, as well as water solubility are given in Tables 5 and 6, respectively.

             Table 4.  Examples of correlations between the adsorption distribution
                     coefficient and the octanol-water partition coefficient	
        Compounds
        Substituted ureas
        Aromatic chlorinated

        Various pesticides

        Aromatic  hydrocarbons
                              Correlations
                              Kom = 0.69+ 0.52 log Pow
                              KoC =-0.779+0.904 log P0,

                              log Kom = 4.4 + 0.72 log POT

                              Koo = -2.53+ 1.15 log POT
 Authors
 Briggs (reference (j)(28))
 Chiou et al. (reference (j)(57))

 Gerstl and Mingelgrin (reference (j)(58))

 Vowles and Mantoura (reference (j)(59))
                                              21

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                   Table 5.  Examples of correlations between the adsorption
                  	distribution coefficient and water solubility	
Compounds
Correlations
Authors
 Various pesticides

 Aliphatic, aromatic chlorinated
 substances
 a-naphtol
 Cyclic, aliphatic aromatic substances

 Various compounds
 log Kom= 3.8-0.561 log Sw

 Kom = (4.040 +/- 0.038) - (0.557 +/- 0.012)
 logSw
 log Koc = 4.273 - 0.686 log Sw
 log Koc = - 1.405 - 0.921 log Sw - 0.00953
 (mp-25)
 Kom = 2.75-0.45 log Sw
 Gerstl   and  Mingelgrin
 G)(58)
 Chiouetal. (j)(62)

 Hasset et al. (j)(63)
 Karickhoff ((j)(33)

 Moreale van Blade (j)(64)
       (2) Calculation of adsorption  A(%) and  desorption  D(%).  (i) Time scheme.  The time
scheme of the procedure is shown in Figure 3 :

Fig. 3. — Time Scheme for calculation of adsorption A(%) and desorption D(%).
                 Ati     At2                      Atari      AtB            ^

                                                                  T
                                                        ta-1
                                                                          Time t
       (Note. For all the calculations it is assumed that the test substance is stable and does not
adsorb significantly to the container walls.)

       (ii) Adsorption A(%). (A) Parallel method. (1) The percentage adsorption is calculated for
each test tube (i) at each time point (t;), according to the equation:
Equation 7
=
                                  =  mf (Q.100  „,
                                          mo
(2) The terms of this equation may be calculated as follows:
Equation 7A
                                 mo = Co • V0 (//g)
                           act ft.  \ _        ri ads /.  \   T 7   / .,  \
                          is  t*,)-  mo  - C   (tt) •  Vo  C"g)
where:
                                              22

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Mo

Co
V0
   =   adsorption percentage (%) at the time point t;;

   =   mass of test substance on soil at the time t; that the analysis is performed (|ig);

   =   mass of test substance in the test tube, at the beginning of the test (|ig);
   =   initial mass concentration of the test solution in contact with the soil (jig cm"3);

   =   mass  concentration of the substance in the aqueous phase at the time  t; that the
       analysis is performed (jig cm"3); this concentration is analytically determined taking
       into account the values given by the blanks.
   =   initial volume of the test solution in contact with the soil (cm3).
       (3) The values of the adsorption percentage At or C^t;) are plotted versus time and the
time after which the  sorption equilibrium is attained is determined.  Examples of such plots are
given in Figures 4 and 5,  respectively.
      •c
     o
    •c
     a.
     o
     VI
    -a
     4*
100-

 90-

 80-

 70-

 60-

 50-

 40-

 30-

 20-

 10-
                    10    15   20    25   30   35   40
                        Equilibration time t. (h)
                                                    45
                                                         50
Fig. 4 Adsorption Equilibrium Plot
                                             23

-------


CO
s

W)
o"
u5
=
o
•^
i.,
4*
W
o
U
100 -;
90-
i
80-

70-
60-
50-
40-
30-
20-

10-
0-

.
» « — c
. 0
1

1
X
X ;
^». C*ds(eq) -
*" 	 .^^ aq v '••
	 *• 	 	 ««»«— •"- - :

-

           0    5    10   15   20   25   30   35   40   45   50
                       Equilibration time t. (h)

Fig. 5 Mass concentration of the test substance in the aqueous phase (Caq) versus time

       (B) Serial method. (1) The  following equations take into  account  that the adsorption
procedure is carried  out by measurements of the test substance in small aliquots of the aqueous
phase at specific time intervals.

       (2) During each time interval the amount of the substance adsorbed on the soil is calculated
as follows:


       -for the first time interval Ati  = ti - to


Equation 8
       - for the second time interval At2 = t2 -
Equation 9
                                        vn
                                       .v.
%-vf
                                            24

-------
       - for the third time interval Ats =


Equation 10
       - for the n* time interval Atn = tn - tn-i
Equation 11
                                                      .
                                                                    A
       (3j  The percentage of adsorption at  each time interval, AAt. ,  is  calculated using the

following equation:




Equation 12


       (This equation is applicable to both direct and indirect methods.  All other equations are

applicable only to indirect method.)
                             AAI =
                                      m0
while the percentage of adsorption ( At ) at a time point t; is given by equation 13.
Equation 13



(This equation applicable to both direct and indirect methods. All other equations are applicable

only to indirect method.)



                                     At;
                                     ^ i   ads /• \


                             At  =^	100
                                1       m
                                            25

-------
       (4) The values of the adsorption At  or AAt  (with respect to the needs of the study) are
plotted versus time and the time after which the sorption equilibrium is attained is determined.
       (5) At the equilibration time teq:


       - the mass of the test substance adsorbed on the soil is shown as equation 14:


Equation 14

       (This equation is applicable to both direct and indirect methods.  All other equations are
applicable only to indirect method.)


                               mru(eq)=5>1«u(Ati)
                                         Ati=l
       - the mass of the test substance in the solution is shown as equation 15.


  Equation 15


       (This equation  applicable to both direct and  indirect methods.   All other equations are
applicable only to indirect method.)
                                            At:=l
       - and the percentage of adsorption at equilibrium is shown as equation 16:
Equation 16

       (This equation is applicable to both direct and indirect methods.  All other equations are
applicable only to indirect method.)
                                     mf(eq)
                                       m0
       - The parameters used above are defined as
                                            26

-------
mads (Atj), mads (At2),...  , mads (Atn)   =  Mass of the substance adsorbed on the soil during the
                                        time intervals Ati, At2,..., Atn respectively (ng);
mads(t ), mads(t ),... ,mads(t )        =  Mass of the substance measured in an aliquot (v^) at
                                        the time points ti, t2,..., tn respectively (ng);
mads(eq)                             =  Mass of the substance adsorbed on the soil at
                                        adsorption equilibrium (ng);
mads(eq)                             =  mass of the substance in the solution at adsorption
                                        equilibrium (|j,g);
VA                                 =  volume of the aliquot in which the test substance is
                                        measured (cm3);
AAt                                =  percentage of adsorption corresponding at a time
                                        interval At; (%);
Aeq                                 =  percentage of adsorption at adsorption equilibrium
       (iii) Desorption  D(%). The time to that the desorption kinetics experiment begins, is
considered as the moment that the maximal recovered volume of the test substance solution (after
that the adsorption equilibrium is attained) is replaced by an  equal volume of 0.01 M CaCb
solution.
       (A) Parallel method. (1) At a time point t;, the mass of the test substance is measured in
the aqueous phase taken from the tube i (Vr'), and the mass desorbed is calculated according to
equation 17.
Equation 17


                             des        des    fVQ^


At desorption equilibrium t; = teq and therefore m*s (t;) = m^s (eq).

The mass of the test substance desorbed during a time interval (At;) is given by equationlS.


Equation 18
       (2) The percentage of desorption is calculated:
                                            27

-------
       - at a time point t; from equation 19.


Equation 19
   1
       - and during a time interval (At;) from equation 20:


Equation 20
                                    mf(eq)

where:


Dt.          =  desorption percentage at a time point t; (%);

DAt.         =  desorption percentage corresponding to a time interval At; (%);

mdes (t )      =  mass of the test substance desorbed at a time point t; (|ig);
  3(J V 1 /

m*s (At;)    =  mass of the test substance desorbed during a time intervals At;
mdes(t x      =  mass of the test substance analytically measured at a time t; in a solution volume
                Vr', which is taken for the analysis (|j,g);
  A         =  mass of the test substance left over from the adsorption equilibrium due to
  aq            incomplete volume replacement (|j,g) is shown as equation 21;

Equation 21
                               aq
  ads
  aq
(eq)    ~ mass of the test substance in the solution at adsorption equilibrium
VR          =  volume of the supernatant removed from the tube after the attainment of adsorption
                equilibrium and replaced by the same volume of a 0.01 M CaCb solution (cm3);
V'          =  volume of the solution taken from the tube (i) for the measurement of the test
                substance, in desorption kinetics experiment (cm3).
The values of the desorption Dt.  or DAt  (according to the needs of the study) are plotted versus
time and the time after which the desorption equilibrium is attained is determined.

       (B) Serial method. (1) The following  equations take into account that the adsorption
procedure, which was preceded, was carried out by measurements  of the test substance in small
                                           28

-------
aliquots ( v^ ) of the aqueous phase.  It is assumed that: a) the volume of the supernatant removed
from the tube after the adsorption kinetics experiment was replaced by the same volume of 0.01 M
CaCb solution (VR  ) and b) the total volume of the aqueous phase in contact with the soil (Vx)
during the desorption kinetics experiment remains constant and is given by equation 22.
Equation 22
       (2) At a time point t; :
       - the mass of the test substance is measured in a small aliquot ( vf ) and the mass desorbed
is calculated, according to equation 23 :
Equation 23
       - At desorption equilibrium t; = teq and therefore m^s (t; ) = m^s (eq) .
       - The percentage of desorption Dt  is calculated, from equation 24:
Equation 24
                              D
                                     des
                                   mf(eq)
       (3) At a time interval (At;):
       - During each time interval the amount of the substance desorbed is calculated as follows:

          •   for the first time interval Ati = ti - to

Equation 25

and
Equation 26
                                           29

-------
              for the second time interval At2 = t2 - ti, shown in equation 27:
Equation 27
                             VT
                                                 VT
                                                         -m
                                                             aq
and equation 28


Equation 28
                     msdes(t2) = mf (eq)-[mJs(At1)
                                             VT
          •   for the nfc time interval Atn = tn - tn_i (equation 29)
Equation 29
  des
 maq (Atn) =
deS/i ,   VT    _ A
D I - maq
                                  (vT-(n-i).v°)
des
aq (
and equation 30


Equation 30
       - Finally,  the percentage  of desorption at each time interval, DAt.  is calculated using

equation 31:
                                           30

-------
Equation 31
                            DAt  =   aaV  '  -100   r/o
while the percentage of desorption Dt at a time point t; is given by equation 32:
Equation 32
                           At:
                           mads(eq)
                                    -•100  =
            msads(eq)
where the above used parameters are defined as:

mf^Atj), msdes(At2),... ,msdes(Atn)   = mass of the substance remaining adsorbed on the soil after
                                    the time intervals Ati, At2,..., Atn respectively (ng);
md^(Atj), md^s(At2),...  ,md^(Atn)    = mass of the test substance desorbed during the time
                                    intervals Ati, At2,..., Atn respectively (ng);
m^^), mdes(t2),...  ,mdes(tn)     = mass of the substance measured in an aliquot (v°) at time
                                    points ti, t2,..., tn , respectively (ng);
VT
m
  aq
=  total volume of the aqueous phase in contact with the soil
   during the desorption kinetics experiment performed with
   the serial method (cm3);
=  mass of the test substance left over from the adsorption
   equilibrium due to incomplete volume replacement (|j,g)
   (equation 33)
Equation 33
                                    m
                                      aq
                                               v0->X(i)
                                                    z_
                                                    1=1
VR
   volume of the supernatant removed from the tube after the
   attainment of adsorption equilibrium and replaced by the
   same volume of a 0.01 M CaCb solution (cm3);

-------
v°                               = volume of the aliquot sampled for analytical purpose from
                                     the tube (i), during the desorption kinetics experiment
                                     performed with the serial method (cm3);
                                     v° < 0.02 •  VT


       (3) Adsorption (i) The adsorption At. is  defined as the percentage of substance adsorbed
on the soil related to the quantity present at the beginning of the test, under the test conditions.  If
the test substance is stable and does not adsorb significantly to the container wall, At. is calculated
at each time point t;, according to equation 34.

Equation 34

A  = mfft.) • 100
             mo

      where:

       At.       =   Adsorption percentage at the time point t; (%);
       msads (t.)  =   mass of test substance adsorbed on the soil at the time t; (jig);
       MO       =   mass of test substance in the test tube, at the beginning of the test (jig).
       Detailed information how to calculate the  percentage of adsorption At. for the parallel and
serial methods is given in paragraph (h)(2) of this guideline.

       (ii).The distribution coefficient Kd is the ratio between the content of the substance in the
soil phase and the mass concentration of the  substance in the aqueous solution, under the test
conditions, when adsorption equilibrium is reached, as shown in equation 35:

Equation 35


^  _  Cf(eq)   mads(eq)  V0      -   -
        Cads(eq)   mads(eq) msoil
       where:
       Cads (eq)    =   content of the substance adsorbed on the soil at adsorption equilibrium (jig
                      g1);
       Cads(eq)    =   mass concentration of the substance in the aqueous phase at adsorption
                      equilibrium (jig cm"3); this concentration is analytically determined taking
                      into account the values given by the blanks.
       mads (eq)    =   mass of the test substance adsorbed on the soil at adsorption equilibrium
                                            32

-------
       mf8 (eq)   =   mass of the test substance in the solution at adsorption equilibrium (jig);
       msoii       =   quantity of the soil phase, expressed in dry mass of soil (g);
       Vo         =   initial volume of the aqueous phase in contact with the soil (cm3).

       (iii) The relation between Aeq and Kd is given by equation 36.

Equation 36

                         Kd  =     Aeq     .  -5^_   (cmV)
                                100 - Aeq    msoil

       where:

       Aeq        =   percentage of adsorption at adsorption equilibrium, %.
       (iv)  The organic  carbon normalized adsorption coefficient Koc  relates  the  distribution
coefficient Kd to the content of organic carbon of the soil sample is shown in equation 37.

Equation 37
                                         100   .   3  K
                                 —  iy           / ^»«^»J -TT. 1\
                              oc  ~~  JVd
                                         %oc

where:

       %oc       =   percentage of organic carbon in the soil sample (g g"1).

          (v) Koc coefficient represents a single value which characterizes the partitioning mainly
of non-polar organic chemicals between the organic carbon in the soil or sediment and water.  The
adsorption of these compounds is correlated with the organic content of the sorbing solid  (see
paragraph (j)(69) of this guideline); thus, Koc values depend on the specific characteristics of the
humic fractions which differ considerably in sorption capacity, due to differences in origin,
genesis, etc.

       (4) Adsorption isotherms, (i) The Freundlich adsorption isotherms equation relates the
amount of the  test substance adsorbed to the  concentration of the  test substance in solution  at
equilibrium (equation 38).
       These data are treated as under "Adsorption" and, for each test tube, the content of the test
substance adsorbed on the soil after the adsorption test (Cads(ecl); elsewhere denoted as x/m)  is
calculated.  It  is assumed that  equilibrium has been  attained and that Cads(eq) represents the
equilibrium value:
                                             33

-------
Equation 38
                               m
                                 soil
       (ii) The Freundlich adsorption equation is shown in equation 39:
Equation 39
                        Cfs(eq)  =
                                                 l/n
       - or in the linear form (equation 40):
Equation 40
where:
                          r (eq) = log Kf + l/n - logCJs (eq)
       K
         ads
                                                                       3  -1
N
          = Freudlich  adsorption coefficient;  its dimension is cm g" only if l/n = 1;  in
                   all other cases, the slope l/n is introduced in the dimension of K* s (|j,g
                                                                                          l-l/n
                = Regression  constant; l/n generally ranges between 0.7-1.0,  indicating that
                   sorption data is frequently slightly nonlinear.

       (iii) Equations (39) and (40) are plotted and the values of Kpdsand l/n are calculated by
regression analysis using the equation 40. The correlation coefficient r2 of the log equation is also
calculated. An example of such plots is given in Fig. 6.
Fig. 6  Freundlich Adsorption Plot, normal (left) and linearized
                                             34

-------
       (5) Mass balance, (i) The mass balance (MB) is defined as the percentage of substance
which can be  analytically  recovered after an  adsorption test versus the nominal  amount of
substance at the beginning of the test.
       (ii) The treatment of data will differ if the solvent is completely miscible with water. In the
case of water-miscible solvent, the treatment of data described under "Desorption" may be applied
to determine the amount of substance recovered by solvent extraction.   If the solvent is less
miscible with water, the determination of the amount recovered has to be made.
       (iii) The mass balance MB for the adsorption is calculated in equation 41. It is assumed that
the term (mE)  corresponds to the sum of the test chemical masses extracted from the soil  and
surface of the test vessel with an organic solvent:
       Equation 41
                      MB  =       -
                                       Vo •  Co
       where:

       MB      =  mass balance (%):
       mE       =  total mass of test substance extracted from the soil and walls of the test vessel
                    in two steps (jig);

       Co       =  initial mass concentration of the test solution in contact with the
                    soil (|ig cm"3);

       Vrec      =  volume of the supernatant recovered after the adsorption equilibrium (cm3).

       (6) Desorption. (i) The desorption is defined as the percentage of the test substance which
is desorbed, related to the quantity of substance previously  adsorbed, under the test conditions
(equation 42).  Detailed information on how to calculate the percentage of desorption Dt; for the
parallel and serial methods is given in paragraph (h)(2) of this guideline.
Equation 42
                              D, =
                                 '
where:
       Dt         =   Desorption percentage at a time point t;  (%);

       mdes (t )    =   mass of the test substance desorbed from soil at a time point t;, (|ig);
         tKJ V 1 s
       m»ds (eq)   =   mass of the test substance adsorbed on soil at adsorption equilibrium (jig).
                                            35

-------
        (ii)  The apparent desorption coefficient (Kdes) is, under the test conditions, the ratio
between the content of the substance remaining in the soil phase and the mass concentration of the
desorbed substance in the aqueous solution, when desorption equilibrium is reached (see equation
43).
Equation 43

                             m:ds(eq)-m;s(eq)   VT    .   3  ,
                     Kdes  =  	d	—  (cm g  )

       where:

       Kdes        =   Desorption coefficient (cm3 g"1);

       mdes (eq    =   total mass of the test substance desorbed from soil at desorption equilibrium
       VT         =   total volume of the aqueous phase in contact with the soil during the desorption
                      kinetics test (cm3).

       (iii)  Guidance  for calculating them*s(eq) is given  in  paragraph  (h)(2)(iii)  of this
guideline under the heading "Desorption".

       Note: If the adsorption test which was preceded, was performed with the parallel method
the volume VT in equation 43 is considered to be equal to VQ.

       (7)  Desorption isotherms, (i) The Freundlich desorption isotherms equation relates the
content of the  test substance remaining adsorbed  on the soil to the concentration of the test
substance in solution at desorption equilibrium (equations 44, 45 and 46).

       (ii)  For each test  tube, the  content of the substance remaining adsorbed on the soil at
desorption equilibrium is calculated as follows:
Equation 44
where m^s (eq) is defined as:

Equation 45

                        m

where:
                                            36

-------
       Csdes (eq)   =    content of the test substance remaining adsorbed on the soil at desorption
                      equilibrium (jig g"1);

       m ^ (eq)  =    mass of substance determined analytically in the aqueous phase at desorption
                      equilibrium (jig);

       mA       =    mass of the test substance left over from the adsorption equilibrium due to
                      incomplete volume replacement (ng);
         ads
         aq
(eq)  ~   mass of the substance in the solution at adsorption equilibrium (|J,g);
      Eq. 46


                                                      'Vn-'
                                                      V   Vo   )

       VF        =   volume of the solution taken from the tube for the measurement of the test
         r                                                 o
                     substance, at desorption equilibrium (cm );
       VR        =   volume of the supernatant removed from the tube after the attainement of
                     adsorption equilibrium and replaced by the same volume of a 0.01 M CaCb
                     solution (cm3);
       (iii) The Freundlich desorption equation is shown in Equation 47:


Equation 47

  Cf(eq)= Kf . Cj(eq)1/n  fag1)

       - or in the linear form as equation 48:
Equation 48


  logCdes(eq)  =
where:
       Kdes      =   Freundlich desorption coefficient;

       N         =   regression constant;
                                           37

-------
       C*s (eq)   =   mass concentration of the substance in the aqueous phase at desorption
                      equilibrium (|ig cm"3).

       (iv)  Equations 47 and 48 can be plotted and the value of KFes  and 1/n are calculated by
regression analysis using equation 48.

       Note: If the Freundlich adsorption or desorption exponent 1/n is  equal to 1, the Freundlich
adsorption  or desorption binding constant  (Kpds  andKpes) will  be  equal to the  adsorption  or
desorption equilibrium constants (Kd and Kdes) respectively, and plots of Cs vs Caq will be linear.  If
the exponents are not equal to  1, plots of Cs vs Caq will  be nonlinear and the adsorption and
desorption constants will vary along the isotherms.

       (8) Additional data reporting considerations, (i) Methods for the preliminary study and
the main study are generally the same, exceptions are mentioned where relevant in this guideline.

       (ii) For the purpose of the test, it is considered that the weight of 1 cm3 of aqueous solution
is Ig. The soil/solution ratio may be expressed in units of w/w or w/vol with the same figure.

       (iii)  The weight  of soil  samples in  the equations of the guideline refer to the oven dry
weight.

       (i) Test report (1) Detailed information on the history of the field site including; location,
vegetation  cover, treatments  with pesticides and/or fertilizers, biological additions or accidental
contamination.   Recommendations  of  the  ISO  standard  on soil sampling   (j)(42) should be
followed with respect to the description of the sampling site.

       (2) Complete identification of the soil samples used including:

       (i) Geographical reference of the site (latitude, longitude).

       (ii) Date of sampling.

       (iii) Use pattern (e.g.,  agricultural soil, forest, etc.).

       (iv) Depth of sampling.

       (v) Sand/silt/clay content.

       (vi) pH values (in 0.01 M CaCl2).

       (vii) Organic carbon content.

       (viii) Organic matter content.

       (ix) Nitrogen content.

       (x) C/N ratio.
                                             38

-------
       (xi) Cation Exchange Capacity (meq/100 g).

       (xii) All information relating to the collection and storage of soil samples.

        (xiii)  Where  appropriate,   all  relevant  information  for  the  interpretation  of  the
adsorption/desorption of the test substance.

       (xiv) Reference of the methods used for the determination of each parameter.

       (3) Information on the test substance as appropriate.

       (4) Temperature of the experiments.

       (5) Centrifugation conditions.

       (6) Analytical procedure used  to analyze the test substance.

       (7) Justification for any use of solubilizing agent for the preparation of the stock solution of
the test substance.

       (8) Explanations of corrections made in the calculations, if relevant.

       (9) All information and observations helpful for the interpretation of the test results.

       (10) Data according to the following suggested form sheets and graphical presentations:
                                             39

-------
Adsorption-Desorption in Soils: Data Reporting Sheets
Substance tested:
Soil tested:
Dry mass content of the soil (105 °C, 12 h):	%
Temperature:	°C
                          Table 6. Suitability of the analytical method
Weighed soil
Soil: dry mass
Volume of CaQ2 sol.
Nominal cone, final sol.
Analytical cone. Final sol.
g
g
cm3
lag cm'3
lag cm"3





Principle of the analytical method used:
Calibration of the analytical method:
                                            40

-------
Substance tested:
Soil tested:
Dry mass contest of the soil (105 °C, 12 h):
Temperature:	°C

Analytical methodology followed:

   o  Indirect
   o  Direct
   o  Parallel
   o  Serial
                            Table 7 Adsorption test: test samples

Tube No.
Weighed soil
Soil: dry mass
Water vol. in weighed
soil (calculated)
Vol. 0.01MCaCl2to
equilibrate soil
Vol. stock solution
Total volume of aq.
phase in contact with
soil
Initial concentration
Test solution
Mass test subst. at
beginning of test
Symbol

-
msoii
Vws


V0
Co
Mo
Units

g
g
cm3
cm3
cm3
cm3
lag cm"3
M«
Equilibration
Time


















Equilibration
Time


















Equilibration
Time


















Equilibration
Time


















After agitation and centrifugation
Indirect Method
Parallel method
Cone, test subst. aq.
phase Blank correction
included
Oi)
lag cm"3








Serial method
Measured mass test
subst. in aliquot vf
Direct method
Mass test substance
adsorbed on soil
Calculation of adsorj
Adsorption
Means
Adsorption coefficient
Means
Adsorption coefficient
m^Cti)

mfao
Hg

Hg
























)tion
At,
AAt,

Kd

Koc
%
%

cm3 g"1

cm3 g"1








































                                            41

-------
Means
                                             42

-------
Substance tested:
Soil tested:
Dry mass content of the soil (105 °C, 12 h):
Temperature:	°C
                         Table 8. Adsorption test: blanks and control

Tube No.
Weighed soil
Water amount in weighed soil
(calculated)
Volume of 0.0 !MCaCl2
solution added
Volume of the stock solution
of the test substance added
Total volume of aq. phase
(calculated)
Initial concentration of the test
substance in aqueous phase
Symbol







Units

g
cm3
cm3
cm3
cm3
lag cm"3
Blank




0







0


Blank















Control

0
-


-



0
-


-

After agitation and eentrifugation
Concentration in aqueous
phase

lag cm"3






Remark:  Add columns if necessary
                                             43

-------
Substance tested:
Soil tested:
Dry mass Content of the soil (105 °C, 12 h):	%
Temperature:	°C
                                  Table 9. Mass balance

Tube No.
Weighed soil
Soil: dry mass
Water volume in weighed soil
(calculated)
Vol. 0.01 M CaCl2 sol. to equilibrate soil
Volume of stock solution
Total vol. of aq. phase in contact with soil
Initial concentration test solution
Equilibration time
After agitation and centrifugation
Cone, test subst. aq. phase at adsorption
equilibrium blank correction included
Equilibration time

Removed volume aq. phase
Added volume of solvent
1st extraction with solvent
Signal analyzer in solvent
Cone, test substance in solvent
Mass subst extracted from soil & vessel walls
2nd dilution with solvent
Removed volume of solvent
Added volume of solvent
2nd extraction with solvent
Signal analyzer solvent phase
Symbol

-
nisoii
Vws


V0
Co
-

Oeq)
teq

vrec
AV

SEI
CEI
niEi

AVS
AV
SE2
Units

g
g
ml
ml
cm3
cm3
lag cm"3
h

lag cm"3
h

cm3
cm3

var.
lag cm"3
M«

cm3
cm3
var.
































































































                                           44

-------

Cone, test subst. in solvent
Mass subst extracted from soil & vessel walls
Total mass test subst. extracted in two
steps
Mass balance
Symbol
CE2
mE2
mE
MB
Units
lag cm"3
Hg
Hg
%




















45

-------
Substance tested:
Soil tested:
Dry mass content of the soil (105 °C, 12 h):
Temperature:	°C
                               Table 10. Adsorption isotherms

Tube No.
Weighed soil
Soil: dry mass
Water volume in
weighed soil (calculated)
Volume 0.0 !MCaCl2
sol. to equilibrate the soil
Volume of stock solution
added
Total volume of aq.
phase in contact with soil
(calculated)
Concentration solution
Equilibration time
Symbol

-
nisoii
Vws


V0
C0
-
Units

g
g
cm3
cm3
cm3
cm3
lag cm"3
h
















































































After agitation and centrifugation
Cone. Test substance in
aqueous phase, blank
correction included
Temperature
Adsorbed mass per unit
soil
.^ads, x
Caq (eq)

/^ads/ s
Cs (eq)
lag cm"3
°C
Hgg1
























Regression analysis:
value of K:
value of 1/n:
regression coefficient r2:
                                            46

-------
Substance tested:
Soil tested:
Dry mass content of the soil (105°C, 12 h):
Temperature:	°C
Analytical methodology followed:
    o  Indirect
    o  Parallel
    o  Serial
                                  Table 11.  Desorption test

Symbol
Units
Tube No. coming from adsorption step
Time
interval

Time
interval

Time
interval

Time
interval


Mass of substance adsorbed on
soil at adsorption equilibrium
Removed volume aq. phase,
replaced by 0.01 MCaCl2
Total volume of aq.
phase
in contact with soil
PM
SM
Mass test subst. left over the
adsorption equilibrium due to
incomplete volume replacement
ads .- x
ms (eq)
VR
V0
VT
<
Hg
cm3
cm3
cm3
M«
















                                            47

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Desorption kinetics
Measured mass of substance
desorbed from soil at time ti
Volume of solution
taken from the tube (i)
for
the measurement of the
test substance
PM
SM
Mass of substance desorbed
from soil at time ti (calculated)
Mass of substance desorbed
from soil during time interval At;
(calculated)
Symbol
=£(0
v;
v°
des / \
maqOj
des / \
maq(AtJ
Units
Hg
cm3
cm3
Hg
M'g
Time
interval





Time
interval





Time
interval





Time
interval





Desorption percentage
Desorption at time ^
Desorption at time interval At;
Apparent desorption coefficient
Dt,
DAt,
Kdes
%
%













PM: Parallel method
SM: Serial method
       (j) References.  The following references should be consulted for additional background
information on this guideline.

       (1).  Calvet, R. (1989). Evaluation of adsorption coefficients and the prediction of the
mobilities of pesticides in soils. In Methodological Aspects of the Study of Pesticide Behavior in
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       (2).  Calvet, R (1980), "Adsorption-Desorption Phenomena" in Interactions between
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       (3).  Hasset J.J., and Banwart W.L., (1989), "The sorption of nonpolar organics by soils
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       (4).  van Genuchten M. Th., Davidson J.M., and Wierenga P.J., (1974), "An evaluation of
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       (5).  McCall, P.J., D.A. Laskowski, R.L. Swann , and HJ Dishburger (1981). Measurement
of sorption coefficients of organic chemicals and their use in environmental fate analysis. In Test
                                           48

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protocols for environmental fate and movement of toxicants. Proceedings of AOAC Symposium,
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                                           49

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                                           50

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                                          51

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