&EPA
              nhsrc
technical  BR
                      Technology  Performance Summary for
                           Chemical Detection Instruments
      Sixteen Instruments Tested to Determine Their Capability to
      Screen Samples Submitted to All Hazards Receipt Facilities
      All Hazards Receipt Facilities (AHRFs) were developed to
      prescreen for chemical, radiochemical, and explosive hazards
      in samples collected during suspected terrorist attacks. The
      technologies (i.e., instruments) used in AHRFs are intended
      to screen samples prior to a full analysis, helping protect
      responders, laboratory workers, and others from potential injury.
      Evaluations of these technologies are summarized in two
      technology evaluation reports:
      1) Testing of Screening Technologies for Detection of Chemical
      Warfare Agents in All Hazards Receipt Facilities (CWAs)
       2) Testing of Screening Technologies for Detection of Toxic
      Industrial Chemicals in All Hazards Receipt Facilities (TICs)
      The chemicals  included in the reports were chosen because
      they might be used during, or develop as a by-product
      from, a terrorist attack.
                                      EPA's National Homeland Security
                                      Research Center (NHSRC) develops
                                      products based on scientific research
                                       and technology evaluations. Our
                                      products and expertise are widely used in
                                      preventing, preparing for, and recovering
                                      from public health and environmental
                                      emergencies that arise from terrorist
                                      attacks. Our research and products
                                      address biological, radiological, or
                                      chemical warfare agents that could affect
                                      indoor areas, outdoor areas, or water
                                      infrastructures. NHSRC rigorously tests
                                      technologies against a wide range of
                                      performance characteristics,
                                      requirements, and specifications.
                                      Technology testing and evaluation is
                                      an effort to provide reliable information
                                      regarding the performance of
                                      commercially available technologies
                                      that may have application for homeland
                                      security.
      The screening technologies are intended:
         • To be rapid and qualitative
         • To be simple to use and of relatively low cost
         • To indicate if samples contain hazardous chemicals of concern.
      Not all of the technologies evaluated were deemed suitable for the AHRF, although they might be
      useful for on scene responders.

      Technology Descriptions
      The screening technologies tested were chosen based on a
      review of commercially available detection devices. From the
      variety of detection instruments reviewed, 16 screening
      technologies were selected for testing based on their
      suitability for use in AHRFs.
      The 16 technologies  ranged from simple test papers, kits,
      and color-indicating tubes to hand-held electronic detectors
      based on ion mobility spectrometry (IMS), photoionization
      detection (PID),  and flame spectrophotometry (FSP). Each
      technology was  tested with three replicate samples for each
      matrix (vapor, liquid,  or on a surface) containing either a
      CWA or TIC. CWAs and TICs were tested at concentrations
         This document does not constitute nor should be construed as an EPA endorsement of any particular product,
         service, or technology.

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 known to be hazardous to humans within a few minutes of exposure (e.g., AEGL = Acute Exposure
 Guide Level (www.epa.gov/opptintr/aegl) and RDT&E = Research, Development, Test, and
 Evaluation Standards (Chemical Surety, Chapter 6: Army Regulation 50-6, 26 June 2001)).
 The following performance parameters were evaluated for each technology:
     •  Identifying the number of false positives/false negatives and the repeatability of test results
     •  Time in which the instrument detected the presence of a chemical (i.e., response time)
     •  Operational information including ease of use and response indication (e.g., color change
       indicating chemical detection)
     •  Cost including initial, sample, and continuing operating costs.
 Technologies were tested to determine their detection capability for the following hazardous
 chemicals in different matrices:
               Hydrogen cyanide
               Cyanogen chloride
                  Phosgene
                   Chlorine
                Hydrogen sulfide
                    Arsine
                    Sarin
                Sulfur mustard
    Cyanide
Hydrogen peroxide
    Fluoride
      Sarin
  Sulfur mustard
 Nerve agent (VX)
Nerve agent (VX)
 Testing Methodologies
 Each technology was tested with one chemical target agent at a time.

 Vapor Testing - Each screening technology was first sampled (or was exposed to) the clean air
 flow, and any response or indication from the screening technology was noted. After this background
 measurement, the 4-way valve was switched to the challenge plenum to deliver the target gas. The
 sequence of exposure to clean air, followed by exposure to the target gas, was carried out three times
 for each screening technology.

 The test apparatus used to evaluate the technologies allowed both the temperature and relative
 humidity (RH) to be adjusted. For each technology, the test sequence of three clean air blanks
 interspersed with three target gases was conducted under four different conditions (i.e., base
 temperature and RH; elevated temperature and RH; low temperature and RH; and base temperature
 and RH with an interferent, a mixture of hydrocarbons representative of polluted  urban air). Testing
 at the base temperature and RH was conducted first, and if a technology failed under this condition,
 then no tests were conducted using the other three conditions.

 Liguid Testing - For CWAs, testing was conducted for technologies and target agents in liquid
 samples that were diluted in isopropyl alcohol (I PA) or deionized (Dl) water. The detection device
 was tested with three blank samples of the solvent used (IPA or Dl water) and three samples of the
 test solution containing the target agent. If a technology detected the chemical in at least one of the
 three samples in the pure solvent, then the challenge was repeated with a hydrocarbon mixture
 interferent (1% of the total volume) added to both the blank and challenge samples.

 For TICs,  samples were prepared in Dl water, in municipal tap water, and in Dl water containing 3.0%
 sodium chloride by weight to simulate potential interfering sample matrices that might  be encountered.

January 2009
EPA/600/S-09/015
   This document does not constitute nor should be construed as an EPA endorsement of any particular product,
   service, or technology.

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 Each screening technology was tested with three blank samples and with three samples containing
 the TICs. If the instrument failed to detect a TIC in all three challenge samples with the Dl water
 matrix, then no tests were conducted with that TIC in tap or salt water.

 Surface Testing - Testing was conducted for each technology using three blank glass coupons and
 three glass coupons spiked with the nerve agent VX. All tests were conducted at room temperature
 and approximately 50% relative humidity. For those technologies that correctly indicated the
 presence of VX in at least one of these three tests, interference tests were then conducted by
 spiking approximately 1 mg of interferent per coupon onto both the blank and VX-spiked coupons.
 Additionally, for these same technologies, the blank  and spiked coupon tests (without interferent)
 were repeated at the same low and high temperature and relative humidity conditions used in the
 vapor testing.

 Test Results
 Table 1 provides a summary of the detection capability of the screening technologies tested.
 The following summarizes the testing information for each matrix form:
 Vapor
     • Draeger Civil  Defense Kit (CDK) detected 6 of 7 chemicals 100% of the time
     • Sensidyne Gas Detector Tubes detected 5 of 5 chemicals 100%  of the time
     • Draeger Chip Measurement System (CMS) Analyzer, MSA Single CWA Sampler Kit,
      and Nextteq Civil Defense Kit (CDK) detected  4 chemicals 100% of the time (out of 4, 5,
      and 5 chemicals tested,  respectively)
     • Anachemia CM256A1, Safety Solutions HazMat Smart-Stripฎ(SS), and Truetech M183A
      detected 2 of 4 chemicals 100% of the time and Proengin AP4C detected 2 of 6 chemicals
      100% of the time
     • Anachemia C2 and RAE Systems MultiRAE Plus detected 1 chemical  100% of the time
      (out of 5 and 8 chemicals tested, respectively)
     • Smiths Detection APD2000ฎ did not detect either of the 2 chemicals tested 100% of the time.

 Liquid
 Due to the lack of acceptable results, samples that were diluted with isopropyl alcohol for CWA testing
 were not factored into the Table 1 summary results.  One explanation for the lack of acceptable results
 may be that the technologies were not designed for application using non-aqueous solvents.
     • Truetech M272 Water Kit detected 3 of 3 chemicals 100% of the  time
     • Severn Trent Services Eclox™ Strip detected 2 of 2 chemicals 100% of the time
     • Proengin AP4C and Safety Solutions HazMat Smart-Stripฎ detected 1  chemical 100% of
      the time (out of 4 and 5 chemicals, respectively)
     • Anachemia C2, Anachemia CM256A1, and Nextteq CDK did not detect any chemical
      100% of the time (3 chemicals tested).

 Surface
     • All of the tested instruments detected the  presence of VX 100% of the time, regardless
      of temperature, relative humidity, or presence of interferent.

 False Negatives and Positives
 False negative results indicate that the screening technology was not able to detect the presence
 of a chemical known to be present. This information  is factored into the test results provided in
 Table 1 and in the summary information above.

January 2009
EPA/600/S-09/015
   This document does not constitute nor should be construed as an EPA endorsement of any particular product,
   service, or technology.

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 Testing for false positive responses was done using "clean" blank samples (i.e., clean air in the vapor
 testing, pure solvents in the liquid testing, and a clean coupon in the surface testing) or interferent
 blank samples (i.e., samples with the hydrocarbon mixture interferent, but without any test chemical
 present). Few false positives occurred. The following summarizes these occurrences:
 Vapor
     •  False positive sarin responses occurred in all three interferent blank samples using Draeger
       CDK and the MSA Single CWA Kit
     •  One false positive sulfur mustard response occurred in the three interferent blank samples
       using Smiths Detection APD2000ฎ.

 Liquid
     •  As indicated, false positives were observed only in the I PA blank samples, which was likely due
       to incompatibility of the screening technologies with that solvent. Proengin AP4C, in particular,
       responded positively to every I PA blank sample.
 Surface
     •  Two false positive responses occurred using the Proengin AP4C at the high temperature
       and relative humidity condition.
 Repeatability
 Repeatability for the presence of TICs was tested for those instruments yielding quantitative results
 (i.e., Draeger CMS Analyzer, RAE Systems MultiRAE Plus, and Sensidyne Gas Detector Tubes).
 Quantitative results were  recorded for each of the triplicate tests, and repeatability was calculated in
 terms of percent relative standard deviation (% RSD). The following summarizes the test information:
     •  32 of the 40 results had less than 15% RSD
     •  Over half of the results (22 of 40) had less than 10% RSD
     •  Several % RSD values exceeded 20% (e.g., Draeger CMS Analyzer for hydrogen
       cyanide and chlorine).

     Note: The PID principle of the MultiRAE Plus was not necessarily expected to respond to TICs or
     CWAs tested as part of this evaluation (see Table 1); however, it was tested based on the
     instrument's promotion as a general toxic compound detector.

 Conclusions from this testing indicate that these instruments can provide reproducible results;
 however, this cannot be assumed to be the case under different environmental conditions
 (i.e., varying temperature and relative humidity)  or with different concentrations.

 Operational Information
 Table 2 provides operational information on the  16 screening technologies tested. Information
 included in the table includes:
     •  Response time information (seconds or minutes to obtain an instrument response)
     •  Ease of use
     •  Response indication (e.g., detection is indicated by color change)
     •  Initial cost.

 Response and Ease of Use Information
 The speed and simplicity  of the vapor screening process varied widely among the tested
 technologies. Ease of use was not necessarily correlated with instruments' detection capabilities. The
 following provides some general highlights on response time and ease of use for each sample matrix:

January 2009
EPA/600/S-09/015
   This document does not constitute nor should be construed as an EPA endorsement of any particular product,
   service, or technology.

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 Vapor
     • Color-indicating tube technologies were simple to use in principle, but differed in the time
      and difficulty of obtaining samples.
        o  The number of manual pump strokes required to draw in the air sample ranged widely,
           as did the manual effort needed for those technologies requiring multiple pump strokes.
        o  Nextteq CDK used an electric air sampling pump that greatly reduced the physical effort
           needed; however, it still required a few minutes to draw the required sample volume.
     • The three real-time technologies tested (RAE Systems MultiRAE Plus, Proengin AP4C, and
      Smiths Detection APD2000ฎ) provided easy and rapid sample analysis for chemicals in vapor;
      however, there was a wide range in instruments' detection capability.
     • Safety Solutions HazMat Smart-Stripฎ was the simplest technology,  requiring only removal of
      a protective film to expose the indicating patches on the card. The detection response occurred
      within seconds.
     • Color-indicating tubes that require the minimum sample volume are  preferable for use in
      AHRFs. Additionally, the use of an electrical sampling pump is helpful if a large numbers
      of samples are to be screened.
 Liquid and Surface
     • For surface samples, M8, M9, and 3-way indicating papers were especially easy to use
      and responses typically occurred within seconds.
     • For liquid samples, Severn Trent Services Eclox™ Strip and Truetech M272 Water
      Kit were relatively easy to use and responses occurred within minutes.
     • Analysis of liquid and surface samples with Proengin AP4C was relatively rapid because
      the detector's attachments were simple to use.

 During homeland security events, it would be important for the technologies to screen for multiple
 chemicals simultaneously.  Technologies using multiple color-indicating tubes at once provide this
 capability. Proengin AP4C provided multi-chemical detection and could be used to detect chemicals
 in vapor, liquid, and surface samples.

 Cost
 The initial cost of the technologies varied substantially, ranging from a few hundred to a few thousand
 dollars. The two exceptions were Proengin AP4C at a discounted cost of nearly $16,000 and Smiths
 Detection APD2000ฎat a cost of $10,000. Comparing purchase prices of different technologies can
 be misleading. Many of the technologies can screen relatively few samples with the originally supplied
 materials. For example, several technologies that rely on color-indicating tubes initially come with
 only enough tubes to screen 10 to 40 samples. Testing larger numbers of  samples requires additional
 tubes. All technologies tested require consumable items such tubes and batteries. Simple test papers
 are the least expensive, with costs estimated at less than $0.50 per sample. Most technologies tested
 had similar costs per sample, typically ranging from $4 to $20 per sample.
 For more information about the technologies evaluated for use in AHRFs,  or by first responders,
 visit the NHSRC Web site at www.epa.qov/nhsrc, or view the full reports, Testing of Screening
 Technologies for Detection of Chemical Warfare Agents in All Hazards Receipt Facilities at
 www.epa.qov/nhsrc/pubs/600r07104.pdf and Testing of Screening Technologies for Detection
 of Toxic Industrial Chemicals in All Hazards Receipt Facilities at
 www.epa.gov/nhsrc/pubs/600r08034.pdf.
 Principal Investigator: Eric Koglin
 Feedback/Questions: Kathy Nickel (513) 569-7955

January 2009
EPA/600/S-09/015
   This document does not constitute nor should be construed as an EPA endorsement of any particular product,
   service, or technology.




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