EPA/600/R-10/004
                                              January 2010
Web-based Interspecies Correlation Estimation

   (Web-ICE) for Acute Toxicity:  User Manual

                     Version  3.1
             \
         http://www.epa.gov/ceampubl/fchain/webice/
Sandy Raimondo, Deborah N. Vivian, and Mace G. Barron

              U.S Environmental Protection Agency
              Office of Research and Development
     National Health and Environmental Effects Research Laboratory
                    Gulf Ecology Division
                   Gulf Breeze, FL 32561

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                            Reference Web-ICE as:
Raimondo, S., D.N. Vivian, and M.G. Barren. 2010. Web-based Interspecies
Correlation Estimation (Web-ICE) for Acute Toxicity: User Manual. Version 3.1.
EPA/600/R-10/004. Office of Research and Development, U. S. Environmental
Protection Agency. Gulf Breeze, FL.
Disclaimers:
The information in this document has been reviewed in accordance with U.S.
Environmental Protection Agency policy and approved for publication. Approval does
not signify that the content reflects the views of the Agency, nor does mention of trade
names or products constitute endorsement or recommendation for use.
Web-ICE models may vary among versions as model data are updated and quality
criteria refined. Please refer to the user manual available with each version for database
descriptions.

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                                Contents

Abstract	3
Introduction	4
Methods	5
      I. Database Development	5
            Aquatic (Fish and Invertebrates)	5
            Wildlife (Birds and Mammals)	6
      II. Model Development	6
      III.  Model Validation	7
Using the Web-ICE Program	8
      I. Working with Web-ICE Aquatic or Web-ICE Wildlife Modules	9
            Selecting Model Taxa	9
            Estimating Toxicity	10
      II. The Species Sensitivity Distribution (SSD) Module	11
            Generating an SSD:	14
      III.  The Endangered Species Module	14
            Producing an Endangered Species Toxicity Report	14
      IV. Accessing  Model Data	16
Guidance for Model Selection and Use	17
      I. Statistical Definitions	17
      II. Selecting a  Model with Low Uncertainty	18
            Rules of Thumb	18
            Surrogate Species Selection: An Example	19
      III.  Evaluating  Model Predictions	19
      IV. Selecting Predicted Toxicity Values for SSDs	20
      V. Applying Web-ICE in Ecological Risk Assessment (ERA)	20
Acknowledgements	22
References	22
Appendices	24

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Appendix I. Summary of acceptance requirements for data included in ICE
models	24
Appendix II. List of Species in Aquatic Database	26
III. List of Species in Wildlife Database	30

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                                  Abstract
      Predictive toxicological models are integral to ecological risk assessment
because data for most species are limited. Web-based Interspecies Correlation
Estimation (Web-ICE) models are least square regressions that predict acute toxicity
(LC50/LD50) of a chemical to a species, genus, or family based on estimates of relative
sensitivity between the taxon of interest and that of a surrogate species. Web-ICE 3.0
includes a total 1440 models for aquatic taxa and 852 models for wildlife taxa. For
aquatic  species within the same family, Web-ICE models predict within 5-fold and 10-
fold of the actual value with 91 and 96% certainty, respectively. For two species within
the same order, aquatic models  predict within 5-fold and 10-fold of the actual value with
86 and 96% certainty, respectively. Overall for wildlife species,  Web-ICE predicts
toxicity within 5-fold of the actual value with 85% certainty and within 10-fold of the
actual value with 95% certainty.  Models predict within 5-fold and 10-fold of the actual
value with 90 and 97% certainty for wildlife surrogate and predicted taxa within the same
order. For both aquatic and wildlife taxa, model certainty increases with decreasing
taxonomic distance. Web-ICE 3.0 improves on earlier versions  with the inclusion of an
endangered species module, improved functionality of the SSD module, and more
rigorous standardization of toxicity data.

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                               Introduction
      Information on the acute toxicity to multiple species is needed for the assessment
of the risks to, and the protection of, individuals, populations, and ecological
communities. However, toxicity data are limited for the majority of species, while
standard test species are generally data rich. To address data gaps in species
sensitivity, the Interspecies Correlation Estimations (ICE) application was developed by
the U.S. Environmental Protection Agency (US EPA) and collaborators to extrapolate
acute toxicity to taxa with little or no acute toxicity data, including threatened and
endangered  species (Asfaw et al. 2003). Web-based Interspecies Correlation
Estimations (Web-ICE) provides interspecies extrapolation models for acute toxicity in a
user-friendly internet platform.
      ICE models estimate the acute toxicity (LC50/LD50) of a chemical to a species,
genus, or family with no test data (the predicted taxon) from the known toxicity of the
chemical to a species with test data (the surrogate species).  ICE models are least
square regressions of the relationship between surrogate and predicted taxon based on
a database of acute toxicity values: median lethal water concentrations for aquatic
species (LC50; ng/L) and median lethal oral doses for wildlife species (LD50; mg/kg
bodyweight). ICE models can be used to estimate acute toxicity when a toxicity is known
for a surrogate species or it can be estimated (e.g., QSAR), and there is an existing ICE
model between the surrogate and taxa of interest (e.g., species-species; species-genus;
species-family).
      In addition to direct toxicity estimation from a surrogate species to predicted taxa,
Web-ICE contains a Species Sensitivity Distribution (SSD) module that estimates the
toxicity of all predicted species available for a common  surrogate. Acute toxicity values
generated by Web-ICE are expressed as a logistic cumulative probability distribution
function in the SSD module to estimate an associated Hazardous Concentration (HC) or
Hazardous Dose (HD) (Dyer et al. 2006). For example,  the HC5 corresponds to the 5th
percentile of the log-logistic species sensitivity distribution and is assumed to be
protective of 95% of tested  species. ICE-generated SSD hazard levels have been
shown to be within an order of magnitude of measured  HC5s (Dyer et al. 2006, Dyer et
al. 2008) and HD5s (Awkerman et al. 2008) and provide additional information for
ecological risk assessment.
      This manual provides step-by-step instructions for using Web-ICE, as well as
information on the expanded databases, model development, model validation, and
user guidance on model selection and interpretation. User guidelines outlined in the
Guidance for Model Selection and Use section  of this manual should be followed to
ensure high confidence and low uncertainty in model predictions used in risk
assessment. Web-ICE 3.0 improves on earlier versions with the inclusion of an
endangered  species module, improved functionality of the SSD module, and more
rigorous standardization of toxicity data.

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                                 Methods
I.  Database Development
Aquatic (Fish and Invertebrates)

      The database of acute toxicity used in development of ICE models included 5501
EC/LC50 values of 180 species and 1266 chemicals. The database was compiled from
the following EPA1 and public domain sources:

   •  US EPA ECOTOX (http://cfpub.epa.gov/ecotox/: accessed February 2009)
   •  US EPA Office of Pesticide Programs ecotoxicity database (accessed January
      2007)
   •  US EPA Office of Water Ambient Water Quality Criteria (US EPA 1986)
   •  US EPA OPPT P re Manufacture Notification (PMN)
   •  US EPA OPPT High Production Volume (HPV) Challenge Program
   •  US EPA Office of Research and Development data sources
   •  Mayer and Ellersieck 1986
   •  Open literature (for list of references, see Raimondo et al. 2008, 2009)

      Data used in model development adhered to standard acute toxicity test condition
requirements of the American Society for Testing and Materials (ASTM 2007, and
earlier editions) and the US EPA Office of Prevention, Pesticides, and Toxic Substances
(US EPA 1996). Data were standardized for test conditions and organism life stage to
reduce variability (Appendix I). In short, selection criteria for aquatic test data were as
follows:

   •  Reported chemical name or structure with chemical active ingredient > 90%
   •  Open-ended toxicity values (i.e. > 100 mg/kg or <100 mg/kg) were excluded
   •  Endpoint was death (LC50) or immobilization (EC50)
   •  48h EC/LC50 for daphnids, midges and mosquitoes; 96h EC/LC50 for fish and all
      other invertebrates
   •  Juvenile only for fish, amphibians, insects, molluscs, decapods; all life stages for
      other groups (Raimondo et al. 2009)
   •  Water quality parameters reported for test condition (e.g., temperature, salinity)
      or confirmation that test conditions met appropriate guideline conditions (e.g.,
      GLP, previously reviewed OPP ecotoxicity data)
   •  Water quality parameters provided for normalization of metals, ammonia and
      pentachlorophenol as directed by Ambient Water Quality Criteria (e.g., AWQC;
      US EPA 1986)
1 All confidential business information (CBI) and data have been censored.

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      When there was more than one toxicity value reported from multiple sources for a
species and chemical, the geometric mean of the values were used. In cases where the
range of minimum and maximum values for a chemical and species were greater than
10-fold, all data records for that chemical were removed for that species due to their
high variability. Toxicity test values for specific compounds were normalized according
to Ambient Water Quality Criteria procedures (e.g., specific metals adjusted to 50 mg/L
hardness; reported on element basis; pentachlorophenol and ammonia were
temperature and pH normalized; US EPA 1986). The resulting aquatic database was
used to develop models to predict toxicity to a species, genus, or family from a surrogate
species (see Appendix II).
Wildlife (Birds and Mammals)

      The wildlife database was comprised of 4329 acute, single oral dose LD50 values
(mg/kg body weight) for 156 species and 951 chemicals. The data were collected from
the open literature (Hudson et al. 1984; Shafer and Bowles 1985, 2004; Shafer et al.
1983; Smith 1987) and from datasets compiled by governmental agencies of the United
States (US  EPA) and Canada (Environment Canada) (Baril et al. 1994; Mineau et al.
2001). Data were standardized by using only data for adult animals and data for
chemicals of technical grade or formulations with > 90% active ingredient. Open-ended
toxicity values (i.e. > 100 mg/kg or <100 mg/kg) and duplicate records among multiple
sources were not included in model development. When data were reported as a range
(ie. 100-200 mg/kg; Hudson et al. 1984) or data were collected from multiple sources for
a species and chemical, the geometric mean of the values was used.  In cases where
the range of minimum and maximum values for a chemical and species were greater
than  10-fold, all data records for that chemical were removed for that species due to
their high variability. Models derived from this wildlife database may be used to predict
toxicity to a species or family from a surrogate species. Genus level models were not
developed from the wildlife database because there were limited genera that had two or
more species (See Appendix III), which is a requirement for development of higher taxa
models.

II. Model  Development

      Models were developed using least squares methodology in which both variables
are independent and subject to measurement error (Asfaw et al. 2003). For species-
level models developed from aquatic and wildlife databases, an algorithm was written in
S-plus (Insightful 2001) to pair every species with every other species by common
chemical. Three or more common chemicals per pair were required for inclusion in the
analysis. For each species pair, a linear model was used to calculate the regression
equation Log-io(predicted toxicity) = a + b*l_og-io(surrogate toxicity), where a and bare
the intercept and slope of the line, respectively. Genus (aquatic only) and family-level
models were similarly developed by pairing each surrogate species with each genus or
family by common chemical. Predicted genera and families required unique toxicity
values for two or more species within the taxon. Toxicity values for the surrogate
species were removed in cases where it was compared to its own genus or family. ICE

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models were only developed between two aquatic taxa or two wildlife taxa; there are no
models to predict toxicity to aquatic taxa from a wildlife species, or vice versa.
      Only models that had a significant relationship (p-value < 0.05) are included in
Web-ICE. The following summarizes the number of significant models developed from
the aquatic and wildlife databases for different taxonomic levels:

      1) Aquatic species: 780 models comparing 77 species to 77 species;
      2) Aquatic genera: 289 models comparing 62 species to 28 genera;
      3) Aquatic family: 374 models comparing 69 species to 27 families;
      4) Wildlife species: 560 models comparing 49 species to 49 species;
      5) Wildlife family: 292 models comparing 49 species to 16 families.
III.  Model Validation

      The uncertainty of each model was assessed using leave-one-out cross-
validation (Insightful 2001). In this method, each pair of acute toxicity values for
surrogate and predicted taxa were systematically removed from the original model. The
remaining data were used to rebuild a model and estimate the toxicity value of the
removed predicted taxa toxicity value from the respective surrogate species toxicity
value. This method could only be used for models with degrees of freedom equal to or
greater than 2 (N > 4). To maintain uniformity among the large number of models
contained within Web-ICE, the "N-fold" difference among each estimated and actual
value was calculated and used to determine the fitness of the estimated toxicity value.
For aquatic species, inter-laboratory variation of acute toxicity test data for a given
species and chemical can be as great as a 5-fold difference (Fairbrother 2008). For
wildlife species, the  average range of multiple toxicity measurements for a specific
chemical and species was determined to be between 4.0 and 6.4 (Raimondo et al.
2007). Thus, a 5-fold difference was deemed a good fit in the validation analysis of both
aquatic and wildlife models.
      The cross-validation success rate was calculated for each model as the
proportion of removed data points that were predicted within 5-fold of the actual value
from models that were statistically significant. In cases where the removal of a xy data
pair resulted in the development of a model that was not significant at the p < 0.05 level,
these replicates were not included in the cross-validation success rate. This is because
models that are not significant at the p<0.05 level have a greater risk of Type I error.
This was only the case for models with low degrees of freedom (<8) and a p-value
between 0.01 and 0.05 in the original model.
      There is a strong relationship between taxonomic distance and cross-validation
success rate, with uncertainty increasing with larger taxonomic distance (Raimondo et
al., 2007). In aquatic species, models predict within 5-fold and 10-fold of the actual value
with 91 and 96% certainty for surrogate and predicted taxa within the same family, and
for 86 and 96% within the same order. In wildlife species, models predict within 5-fold
and 10-fold of the actual value with 90 and 97% certainty for surrogate and predicted
taxa within the same order. Model certainty decreases with increasing taxonomic
distance. A more detailed account of model uncertainty as it relates chemical mode of
action/class is discussed in Raimondo et al. (2007).

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                         Using the  Web-ICE  Program
       The Web-ICE platform contains separate modules that predict acute toxicity to
aquatic (vertebrates and invertebrates) species, genera, or families (ICE Aquatic) and
wildlife (terrestrial birds  and mammals) species or families (ICE Wildlife) (Figure 1). The
Species Sensitivity Distribution Module is available for aquatic and wildlife species and
batch processes species level toxicity from all entered surrogates. The Endangered
Species Module,  also  available for aquatic and wildlife taxa, predicts toxicity to listed
species from all available species, genus, or family level models for the entered
surrogates. Each module is accessible from either the home page or from the blue
navigation bar along the left side of the page. Before working with a Web-ICE module,
you must first decide if you are going to work with aquatic or wildlife taxa, the program
does not contain  models that estimate wildlife toxicity from an aquatic surrogate, or vice
versa.
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      The Web-based Interspecies Correlation Estimation (Web-ICE) apolication estimates acute

      toxicity to aquatic and terrestrial organisms for use in risk assessment Please refer to the

      User Manual for detailed instructions on using Web-ICE
                                                Web-ICE Modules
    ICE Aquatic

Aquatic vertebrates invertebrates
        Suecies
        Genus
        Family
  ICE Wildlife

TerrestriaJ Birds Mammals
      Species
      Family
                                      Species Sensitivity Distribution Module
                                        IC£ Aquatic
                                                                      ICE Wildlife
                                           Endangered Species Module
                                        ICE Aquanc
                                                                      ICE Wildlife
                                       Please address all comments a
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Figure 1. Home page of Web-ICE program

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I. Working with Web-ICE Aquatic or Web-ICE Wildlife Modules
Selecting Model Taxa

   1.  From either the home page or the blue navigation bar, click the link for the
      module with which you will be working (Aquatic species, genus, or family; Wildlife
      species or family).
   2.  You will then be directed to a Taxa Selection Page (Figure 2) which will allow you
      to select your surrogate and predicted taxa for the model.
   3.  You may search for your surrogate and predicted taxa by either common name or
      scientific name by selecting the appropriate option in the Sort by: drop down
      menu. The default is set to common name.
   4.  From the drop down menus, select the surrogate species and predicted taxon.  It
      does not matter which you select first; however, the second choice is limited to
      the models available for the taxon chosen first.
   5.  To change any of your selections, press Reset and start again.
   6.  Click Continue to be directed to the calculator page for toxicity estimation.

      If there is not a model for your predicted species of interest, you will need to use
a genus or family-level model to predict toxicity. The available models may be
determined by browsing through the genus (aquatics only) and family level modules, or
by searching through the spreadsheets of model information available through the
Download Model Data option on the blue navigation bar. The downloadable Microsoft
Excel® spreadsheets provided for each Web-ICE module may be sorted by surrogate
species  or predicted taxa to identify available models.

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                 Aquatic Species - Taxa Selection Page
 Web-ICE Home

 Aquatic Species
 Aquatic Genus
 Aquatic Family
 Wildlife Species
 Wildlife Family

 Species Sensitivity
 Distributions
  ndangered Spec
 Basic Informant)
 Download Model Data
 Bibliography
                  | Rainbow trout (Oncorhynchus mykiss)
Sort By I Common Name H


 Reset I
                                                  Continue
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Figure 2. Taxa selection page
Estimating Toxicity

       The surrogate and predicted species selected from the previous page are listed
at the top of a calculator page (Figure 3). This page is divided into four parts: input,
calculated results, model statistics, and model graphic. The known toxicity for the
surrogate species is entered under Surrogate Acute Toxicity, below which the desired
confidence limits can be selected (Figure 3A). Predicted toxicity estimates and
confidence intervals are displayed under Predicted Acute Toxicity (Figure 3B). The
bottom left side of the page contains the model statistics (Figure 3C). Please refer to
the Statistical Definitions section of this manual for more specific information. The graph
shows the data (LC50/LD50 values) used to develop the model, the regression line
(straight inner line), and 95% confidence intervals  (curved outer lines) (Figure 3D). The
surrogate and predicted taxa are labeled on the X  and Y axes, respectively.  Both the
model statistics and the graph are unique for each model and will change for each
surrogate species and predicted taxon.

    1.  Enter the acute toxicity value in the  box located under Surrogate Acute Toxicity
       (Figure 3A).
    2.  Select your desired confidence interval (90, 95, or 99%) from the drop down
       menu located under Select Confidence Interval (Figure 3A).  The default for the
       confidence intervals is 95%.
                                        10

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    3.  Press Calculate
    4.  The calculated values will appear in the three boxes labeled Predicted Acute
       Toxicity, Lower Limit and Upper limit (Figure 3B).
    5.  Log-transformed values of the surrogate and predicted toxicity values appear in
       parentheses next to the values.
    6.  If the entered surrogate toxicity value is outside the range of toxicity values used
       to develop the model, a pop-up with the warning "This value is outside the x-axis
       range for this model. Continue?" will appear. The user may select "OK" to
       proceed to calculate the toxicity value or hit cancel to enter another value.
    7.  To select a different model, select the link to the desired module in the blue
       navigation bar on left side of the page.
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Calculator - Aquatic Species

               Surrogate Species: Rainbow trout (Oncorhynchus mykiss)

               Predicted Species: Brown trout (Salmo trutta)

Surrogate Acute Toxicity (log value)    Predicted Acute Toxicity (log value)

f?50      yg L(2.17)
                                142-71 ug.LI2.15)
                   Select Confidence Interval:

                   195%
                                                       Upper Limit
                                                  B
                              • 0 jg L
                                       195.65 yg L
                     Calculate
  Download Model Data
  Bibliography
 Model Information

 Intercept:                 0.042271

 Slope:             /"*    0.970642

 Degrees of Freedom (N-2)  ^    17

 R2:                     0.964248

 p-value:                  0.000000

 Average value of surrogate (log value): 1 19.80 (2.07)

 Minimum value of surrogate (log value): 0.1 63864 (-0.78551 5)

I Maximum value of surrogate (log value): 1 7808.08 (4.25)

 Mean Square Error (MSE):        0.079728

 Sum of Squares (Sxx):           38.80

I Cross-validation Success (%):      94.73

I Taxonomic Distance:
                                                         £ &
                                                         '•~f V.<
                                                                       Oncorhynchus mykiss
                                                                          (Log LC50)
Figure 3. Calculator Page

II. The Species  Sensitivity Distribution (SSD) Module

       Species Sensitivity Distributions (SSDs) are probabilistic models that describe
the sensitivity of biological species to a chemical. SSDs generated in Web-lCE are log-
logistic cumulative distribution functions of toxicity values for multiple species (de Zwart
2002) and are used to estimate a hazard level (hazardous concentration (HC) or
hazardous dose (HD)) that is protective of most test species (e.g., 95%) by estimating
                                          11

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the concentration or dose at a corresponding percentile (e.g., 5th) of the distribution
(Dyer et al. 2006).
      The SSD modules for aquatic and wildlife species generate SSDs from Web-ICE
toxicity values estimated from one or more surrogate species. Toxicity values for one or
more surrogate species are used to simultaneously estimate toxicity to all possible
predicted species with existing Web-ICE models. The SSD is then generated using all
estimated toxicity values and the entered toxicity of the surrogate species. Toxicity
values for up to 25 surrogate species may be entered (Figure 4). If more than one
surrogate species estimates toxicity to the same predicted species, Web-ICE selects the
toxicity value with the smallest confidence intervals. If multiple surrogates are used and
a predicted value is estimated for one of the surrogate species, Web-ICE uses the
entered value for that species and excludes the predicted value(s) from the SSD.
      An HC/HD level is automatically calculated from the distribution. The user can
deselect toxicity values for predicted species that they wish to exclude from the SSD by
clicking on the box to the left of the predicted species (Figure 5), and the associated
HC/HD value  is automatically recalculated. An HC/HD drop down menu on the output
page allows the user to specify the hazard level to calculate. HC1/HD1  corresponds to
the 1st percentile, HC5/HD5 corresponds to the 5th percentile, and HC10/HD10
corresponds to the 10th percentile. The default is set to HC5 for aquatic species and
HD5 for wildlife species.

      Web-ICE uses the SSD described by the logistic distribution function of de Zwart
(2002):

                           F(C)=1/(1+exp((a-C)/p))

The logic-transformed environmental concentration (or dose) of the evaluated chemical
is represented by C, the parameter, a, is the sample mean of the log™ -transformed
toxicity values and p is defined as VS/TI * o, where o is the standard deviation of the log-io
-transformed toxicity values (de Zwart 2002). The HC/HD level is determined as the
percentile of interest (e.g., 5  ) of the described distribution. Corresponding SSDs are
also  developed from the upper and lower confidence limits of the predicted toxicity
values and are used to calculate the upper and lower bounds of the HC/HD value at a
given percentile. For example, the lower bound of the HC5 is calculated as the 5th
percentile of the SSD developed from the estimated lower confidence limit of each
predicted toxicity value. Similarly, the upper bound of an HC5 is calculated as the 5th
percentile of the SSD developed from the estimated upper limit of each predicted toxicity
value.
                                    12

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                             Species Sensitivity Distributions  -  Aquatic Species
                             Multiple Surrogate SSD
        Aquatic Species
        Aq  -  ~
        Aquatic Fa
        Wildlife Species
       Species Sensitivity
       Endangered Species
Surrogate:
Sort By | Common Name H
Species

Fathead minnow (Pimeohales promelas

Bluegill (Lepomis macrochirus)

Daphnid (Daphnia magna)

Sheepshead minnow (Cyprinodon

   Calculate SSD  I
                                                             Toxicity
       Download Model Data
s)
lease
|150

|125
ITS

5,lioo
address all com
Remove Species

Remove Species
Remove Species

Remove Species
ments and questions to the A'ets master
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                                                    News by Email  i • ) Widgets
Figure 4. SSD taxa selection page.
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                                                     .posure Assessment » Food Chain » WeblC£ » Aquatic Species » Results

                               Species Sensitivity  Distributions - Aquatic
                                                 Surrogate Species:  Fathead minnow {Pimephales promelas), Bluegill (Lepomis
                                                 macrochirus), Daphnid (Daphnia magna), Sheepshead minnow (Cyprinodon variegatus)
                                                 Input Toxicity: 150, 125, 75, lOOug/L
                                                 |HC5 jj s.44 (jg/L  95% Confidence Interval: 1.44-21.62
                                  Common Name  Scientific          Estimated  95%        Show Data
                                  Sort         Sort              Toxicity   Confidence   Surrogate              ^1
                                                              Sort -     Intervals     sort
                                                                      Sort
                                R Stonefly

                                P" Amphipod

                                W Stonefly

                                W Daphnid
                 PteronarceNa badia  3.66

                 Ceriodaphnia dubia  8.20

                 Gammams lacustris  B.26
                                P Amphipod
                                P Chinook salmon Oncorhynchus
                                              tshawytscha
                                W Amphipod     Gammarus
                                              pseudolimnaeus
                                |7 Shortnose     Acipenser
                                  sturgeon      brevirostrum
                                W Mvsid        Americamvsis bahia  39.30
Figure 5. SSD output page.
"  :; 5     0.460 -4.13 Fathead minnow (Pimephales
                   promelas)
3.58     0.048 -     Daphnid (Daphnia magna)
        266.24
        2.15 - 6.21   Fathead minnow (Pimephales
                   promeias)
        0.769 -     Fathead minnow (Pimephales
        87.56      promelas)
        1.71 - 39.90 Biuegill (Lepomis macrochirus)
1 3.41 Bluegitl (Lepomis macrochirus)

18.39
32.82

39.30
110.04
5.29 - 53 71
8.99 -
119.81
J684-

Bluegill (Lepomis mai
Fathead minnow (Pin
promelas)

:rochirus)
lephales

Daohnid (Dachnia maanai
                                                           13

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Generating an SSD:

   1.  Under the SSD module, select either Aquatic or Wildlife.
   2.  On the SSD taxa selection page, select your surrogate species from the drop
      down menu and click Add to add the species as a surrogate.
   3.  If desired, select additional surrogate species from the drop down menu and click
      Add. A maximum of 25 species can be selected.
   4.  To remove a surrogate species from the list after it is added, click Remove next to
      the species name.
   5.  Enter the known toxicity for the surrogate species,  click Calculate SSD.
   6.  On the SSD output page, the HC/HD level may be  changed from the drop down
      box. The  hazard level is automatically recalculated if the level is changed. The
      default is the HC/HD5.
   7.  The warning "Input toxicity is greater (less) than model maximum (minimum)"
      indicates  if a predicted value was generated from a surrogate species toxicity
      value that was outside the range of toxicity values used to generate that model.
   8.  The user  can unmark the box to the left of a predicted species to exclude it from
      the SSD,  which is automatically recalculated. (NOTE: See Selecting Predicted
      Toxicity Values for SSDs in the Guidance for Model Selection and Use section
      below for guidance on removing estimated toxicity  values).
   9.  The drop  down menu  in the Show Data column provides additional model
      information (surrogate, taxonomic distance, cross-validation success rate,
      degrees of freedom, R2, p-value, or mean square error) for the user to view.
   10. The user  may sort  the ICE-estimated toxicity values by each column by selecting
      the sort tab below the column heading.
III. The Endangered Species Module

      The Endangered Species Module batch processes toxicity values for endangered
species from all species, genus, and family level models available for the entered
surrogates. The list of threatened and endangered species was obtained from the US
Fish and Wildlife Service Threatened and Endangered Species module of
Environmental Conservation Online System (http://ecos.fws.gov/tess_public; Accessed
August 2007), which was linked to Web-ICE species, genus, and family model
databases for aquatic organisms and wildlife. Users may predict to all available
endangered species within a broad taxonomic groups (e.g., Fishes) or a particular
species (e.g., Atlantic Salmon,  Salmo salar] using up to 25 surrogates.
Producing an Endangered Species Toxicitv Report
   1.  Under the Endangered Species module, select either Aquatic or Wildlife.
                                   14

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   2. On the Endangered Species taxa selection page, select either the broad taxa of
      interest (e.g., Fishes) or a particular species of interest from the drop down menu
      (Figure 6).
   3. Select your surrogate species from the drop down menu and click Add to add the
      species as a surrogate. A maximum of 25 species can be selected.
   4. To remove a surrogate species from the list after it is added, click Remove next to
      the species name.
   5. Enter the known toxicity for the surrogate species, click Calculate.
   6. The Endangered species output page provides the estimated toxicity for each
      predicted taxa, the model level (e.g., species), surrogate, and model information
      (Figure 7).
   7. The user may sort the ICE-estimated toxicity values by each column by selecting
      the sort tab below the column heading.
  Interspecies Correlation Estimation
                  You are here: EPA Home » Exposure Assessment » Food Chain » WeolCE » Endangered Species » Aquatic Species
                  Endangered Species Module - Aquatic Species
                  Step 1: select Taxa of Interest
                  *  All Species ** Fishes *  Amphibians *  Crustaceans * Molluscs

                  Species
                  Sort By |Common Name


                  Step 2: select Surrogate(s)

                  Surrogate(s).
                  Sort By | Common Name
                  Fathead minnow (Pimephales promelas) I

                  Daphnid (Daphnia magna)
                                    Please address all comments and questions to Che webmaster
Figure 6. Taxa selection page of Endangered Species module.
                                      15

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 ¥ou  hsre: "4 Hcnis » EKpoiure "-ussssTiei" »*:-"•" ' v- •"• ^

                     -

             Surrogate 5{>e-;t
-------
And confidence intervals as:
     Lower bound = 10A(log(predicted) - ti-a*V[MSE*(1/n + (log(x) - x.ave)A2/Sxx) ])
- ti-a*[MSE*(1/n +
+ t^NIMSE^I/n +
     Upper bound = 10A(log(predicted) + t^NIMSE^I/n + (log(x) - x.ave)A2/Sxx) ])

Where x is the untransformed value of surrogate toxicity, x.ave is the average value of
log-transformed surrogate toxicity values, Sxx is the sum of squared deviations of the
surrogate, MSB is the mean square error, and ti_a is the value of the t distribution
corresponding to the desired level of confidence (ie. 90, 95, 99%).
              Guidance for Model  Selection and Use
I.  Statistical Definitions

      Several statistics are provided with each model and may be used to evaluate the
accuracy and precision of the estimated value. These statistics are shown to the left of
the graph on the calculator page (Figure 3C) and are provided in the spreadsheet of
model information available in the Download Model Data option. The following provides
a basic interpretation of model statistics to help guide users in model selection:

      Intercept - The log™ value of the predicted taxon toxicity when the log™ of the
      surrogate species toxicity is 0.

      Slope - The regression coefficient represents the change in log™ value of the
      predicted taxon toxicity for every change in log™ value of the surrogate species
      toxicity.

      Degrees of Freedom (df,  N - 2) - The number of data points used to build the
      model minus two. Degrees of freedom are related to statistical power; in general,
      the higher the degrees of freedom, the more robust the model.
      R  - The proportion of the data variability that is explained by the model. The
      greater the R2 value and the closer it is to one, the more robust the model is i
      describing the relationship between the predicted and surrogate taxa.
      p-value - The significance level of the linear association and the probability that
      the linear association was a result of random data. Models with lower p-values
      are more robust. Model p-values of <  0.00001 are reported as 0.00000.

      Average value of the surrogate - The average of toxicity values for the surrogate
      species used in  the model. The first number is the actual value and the number in
      parentheses is the log-transformed value.
                                   17

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      Minimum value of the surrogate - The lowest toxicity value for the surrogate
      species used in the model. The first number is the actual value and the number in
      parentheses is the log-transformed value.

      Maximum value of the surrogate - The largest toxicity value for the surrogate
      species used in the model. The first number is the actual value and the number in
      parentheses is the log-transformed value.

      Mean Square Error (MSB) - An unbiased estimator of the variance of the
      regression line.

      Sum of Squares (Sxx) - Sum of squared deviations of the surrogate.

      Cross-validation Success - The percentage of removed data  points that were
      predicted within 5-fold of the actual value. Models with a Cross-validation
      Success of "na" are those that either had df = 1 or where no significant models
      were developed when data points were removed.

      Taxonomic Distance - The taxonomic relationship between the surrogate and
      predicted taxa. Two taxa within the same genus have taxonomic distance of 1;
      within the same family = 2; within the same order = 3; within the same class = 4;
      within the same phylum = 5; within the same kingdom = 6.
II.  Selecting a Model with Low Uncertainty
Rules of Thumb

      Model attributes, such as taxonomic distance of the predicted and surrogate
species, model parameters (listed below) and cross-validation success rate, should be
used to select models with low uncertainty. For best estimates, models should be
selected that possess the following:

                  1.  Relatively low mean square error (MSB) (<0.22)
                  2.  Close taxonomic distance (< 3)
                  3.  High cross-validation success rate (> 85%)
                  4.  High degrees of freedom ( df > 8, N > 10)
                  5.  High R2 value (> 0.6)
                  6.  Low p-values (< 0.01)
                  7.  Narrow confidence bands on the graph

      The best estimations generally occur for surrogate and predicted taxa that are
within the same genus, family, or order and for models with R2  > 0.6 (Raimondo et al.
2007). In general, models with more degrees of freedom (df) have greater statistical
power and choosing a model with df greater than 8 is recommended to reduce model
                                   18

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uncertainty. A priori power analysis determined that linear models with df > 8 have
enough statistical power (1-fi > 0.8) to sufficiently increase the chance of finding a
significant relationship within the data. It is also recommended to choose models with p-
values < 0.01 to further reduce the chance of Type I errors in the toxicity estimations.
      Cross-validation success rate is a conservative estimate of model uncertainty and
should not be interpreted as an exact estimate of model error. Cross-validation removes
data from the original model, potentially causing a large change in the model for small
datasets. Due to changes in a model (i.e. reduced df, altered slope/intercept) during this
validation process, cross-validation success rate should be considered only an estimate
of generalization error. Particularly for models built from small datasets, actual error can
be expected to be lower than cross-validation error.
Surrogate Species Selection: An Example

      In an example of how to select a suitable model, Raimondo et al. (2007) outlined
a selection procedure to find an appropriate surrogate species to estimate the toxicity of
a chemical to red-winged blackbird. In the example, toxicity data for the chemical of
interest was available for northern bobwhite, mallard, Japanese quail, fulvous whistling
duck, common grackle, and house sparrow, making them all potential surrogates. The
common grackle and house sparrow have the closest taxonomic  distance (2, same
family; 3, same order); the other potential surrogates in this example have a taxonomic
distance of 4 (same class). Of the grackle and house sparrow, both have similar MSB
(~0.13), however house sparrow has a higher model R (0.84), higher cross-validation
success rate (95), and greater degrees of freedom (107), and is the best surrogate for
red-winged blackbird in this example. The grackle would also provide good surrogacy,
with high R2 (0.65), high cross-validation success rate (93), and good degrees of
freedom (54). If neither of these species were available surrogates, Japanese quail (R2
= 0.79, MSB = 0.15, df = 135, cross-validation success rate = 91) would be the next best
surrogate, followed by northern bobwhite (R2 = 0.63, MSB = 0.23, df = 45, cross-
validation success rate = 85)  and mallard (R2 = 0.48, MSB = 0.34, df = 80, cross-
validation success rate = 79). Although fulvous whistling duck has the highest model R2,
low degrees of freedom (df =  2) and comparatively higher MSB (0.30) do not make it as
suitable of a surrogate as the other species.
III.  Evaluating Model Predictions

      Uncertainty of model predictions may be evaluated by assessing (1) the
characteristics of the model used in the predictions, and (2) the value of the input data
relative to the data used to generate the model. The former was discussed in the
previous section and the Rules of Thumb should be followed to ensure high confidence
in model selection. Even for robust models, however, model uncertainty increases
outside the range of surrogate species toxicity values that were used to develop the
model.
      Uncertainty may be evaluated by reviewing the confidence intervals calculated
with the predicted value.  Narrow confidence intervals represent higher confidence that
                                    19

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the model fits through the range of datapoints for the entered surrogate species toxicity.
If the surrogate toxicity value entered into an ICE model is outside the range of
surrogate toxicity data used to generate the model, the warning "This value is outside
the x-axis range for this model. Continue?" will appear to alert the user. This warning
alone does not indicate low confidence in the model estimate, but should be used in
conjunction with the calculated confidence  intervals to evaluate the model prediction.
For example, if the upper and lower bounds of the confidence interval are several orders
of magnitude from the predicted value, caution should be used in applying the ICE
estimate in risk assessment.

IV. Selecting Predicted Toxicity Values for SSDs

      The SSD modules of Web-ICE automatically predict toxicity values from all
available models for the selected surrogate species simultaneously. The user has the
discretion to remove predicted toxicity values from the SSD to either customize the SSD
for a particular taxa (e.g., birds only, fish only), or to remove predicted toxicity values
with large confidence intervals. If an estimated toxicity value was derived from an input
value that was outside of the range of surrogate species data used to generate the
model from which it was predicted, a warning appears next to the value indicating the
maximum or minimum value of the model. This warning alone does  not indicate low
confidence in the model estimate, but should be used in conjunction with the calculated
confidence intervals to evaluate the model  prediction.
      Users should also use the confidence intervals around the HC/HD level to guide
the selection of toxicity values to exclude from  the SSD. Cases in which the upper
bound of the SSD is less than the HC/HD level occur when predicted toxicity values with
extremely large confidence intervals are included in the SSD; removal of predicted
toxicity with such confidence intervals results in HC/HD values with adequate
confidence. Users may also refer to the model  information provided  by the Show Data
drop down menu when selecting data to include in SSDs.
V. Applying Web-ICE in  Ecological Risk Assessment (ERA)

      Web-ICE was developed to support both chemical hazard assessment and
ecological risk assessment (ERA) by providing a  method to estimate acute toxicity to
specific taxa, such as endangered species, or to  a larger number of taxa (species,
genera, families) with known uncertainty. Potential applications of acute toxicity values
generated by Web-ICE include the problem formulation phase of an ERA to screen for
contaminants of potential concern and in the analysis phase to characterize effects to a
larger number of species. The estimation of species-specific toxicity values using Web-
ICE is recommended  as an alternative to safety factors typically applied when
extrapolating toxicity or risks to taxa  without chemical and species-specific toxicity data.
Another potential application of the chemical and taxon-specific acute toxicity estimates
generated from ICE models include input into existing exposure and risk models (e.g.,
TREX; EPA 2005). Web-ICE generated toxicity values may also be used in the analysis
                                   20

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of uncertainty and variability in toxicity to ecological receptors in both screening level
and baseline or Tier II ERAs.
      In the absence of taxa-specific ICE models, Web-ICE can be used to generate
SSDs and estimated 1st, 5th or 10th percentile values of the cumulative distribution of
species-specific toxicity values.  These percentile values, expressed as the hazard
concentration (e.g., HC5) or hazardous dose (e.g., HD5),  provide an estimate of toxicity
at a prescribed level of species protection with known uncertainty. Hazard
concentrations could be  used in ERA in place of species-specific toxicity values or as a
component of the uncertainty analysis.
                                    21

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                          Acknowledgements


For database development, the authors would like to thank Sonny Mayer (US EPA,
retired), Thomas Steeger and Brian Montague (US EPA, Office of Pesticide Programs),
Don Rodier (US EPA, Office of Pollution Prevention and Toxics),  Pierre Mineau, Alain
Baril and  Brian Collins (National Wildlife Research Centre, Environment Canada), Chris
Russom and Teresa Norberg-King (US EPA, Mid-Continent Ecology Division), and
Christopher Ingersoll and Ning Wang (Columbia Environmental Research Center, U.S.
Geological Survey). Special thanks to Wally Schwab and  Derek Lane (Computer
Sciences Corporation) for constructing the website, and to Carl Litzinger (US EPA, Gulf
Ecology Division) and David Owens (Computer Sciences Corporation) for their
facilitation of website development. Also, thanks to our support personnel: Marion
Marchetto, Anthony DiGirolamo, Brandon Jarvis, Christel  Chancy, Nathan Lemoine,
Nicole Allard, Laura Dobbins, Cheryl McGill, Sarah Kell, and Crystal Jackson. Peer
review and beta testing of the website were contributed by Larry Goodman,  Michael
Murrell, Raymond Wilhour, and Susan Yee (US EPA, Gulf Ecology Division), Rick
Bennet (US EPA, Mid-Continent Ecology Division), Glen Thursby (US EPA, Atlantic
Ecology Division), and Anne Fairbrother (US EPA, Western Ecology Division).
                                References
American Society for Testing and Materials (ASTM). 2007. Standard guide for
   conducting acute toxicity tests with fishes, macroinvertebrates, and amphibians. E
   729-96(2007). Philadelphia PA.
Asfaw, A., M. R. Ellersieck, and F. L. Mayer. 2003. Interspecies Correlation Estimations
   (ICE) for acute toxicity to aquatic organisms and wildlife. II. User Manual and
   Software. EPA/600/R-03/106. U.S. Environmental Protection Agency, National
   health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf
   Breeze, FL. 14 p.
Awkerman, J., S. Raimondo, and M.G. Barren.2008. Development of Species Sensitivity
   Distributions for wildlife using interspecies toxicity correlation models. Environ. Sci.
   Technol. 42 (9): 3447-3452.
Baril, A., B. Jobin, P. Mineau, and B. T. Collins. 1994. A consideration of inter-species
   variability in the use of the median lethal dose (LDso) in avian risk assessment.
   Technical Report No. 216. Canada Wildlife Service, Headquarters.
De Zwart, D. 2002. Observed regularities in species sensitivity distributions for aquatic
   species. In Species Sensitivity Distributions in Ecotoxicology, L. Posthuma, G.W.
   Suter, T.P.Traas, Eds. Lewis Publishers, Boca Raton, FL. pp133-154.
Dyer, S. D., D. J. Versteeg, S. E. Belanger, J. G. Chaney, and F. L. Mayer. 2006.
   Interspecies correlation estimates predict protective environmental concentrations.
   Environ. Sci. Technol. 40: 3102-3111.
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Dyer, S. D., D. J. Versteeg, S. E. Belanger, J. G. Chaney, S. Raimondo and M. G.
   Barren. 2008. Comparison of Species Sensitivity Distributions Derived from
   Interspecies Correlation Models to Distributions used to Derive Water Quality
   Criteria. Environ. Sci. Technol. 42: 3076-3083.
Fairbrother, A. 2008. Risk Management Safety Factor. In. Encyclopedia of Ecology, vol.
   4. S. E. J0rgensen and B. D. Fath (eds.). Elsevier publishing, pp. 3062-3068.
Hudson, R. H., R. K. Tucker, and M. A. Haegele. 1984. Handbook of toxicity of
   pesticides to wildlife. U.S. Fish and Wildlife Service, Resource Publ. 153,
   Washington  D.C. 90 p.
Insightful. 2001. S-plus 6 Guide to Statistics. Volume 1. Insightful Corporation, Seattle,
   WA.
Mayer, F. L. and M. R. Ellersieck. 1986. Manual of acute toxicity: Interpretation and data
   base for 410 chemicals and 66 species of freshwater animals. US Fish and Wildlife
   Service Resource Publication 160. Washington DC. 579 p.
Mineau, P., A. Baril, B. T. Collins, J. Duffe, G. Joerman, and R. Luttik. 2001. Pesticide
   acute toxicity reference values for birds. Rev. Environ. Contam. Toxicol. 170: 13-74.
Raimondo, S., P. Mineau, and M. G.Barren. 2007. Estimation of chemical toxicity in
   wildlife species using interspecies correlation models. Environ. Sci. Technol. 41:
   5888-5894.
Raimondo, S., D.N. Vivian, C. Delos, M.G. Barren. 2008. Protectiveness of Species
   Sensitivity Distribution Hazard Concentrations for Acute Toxicity Used in
   Endangered Species Risk Assessment. Environ. Toxicol. Chem. 27 (12): 2599-2607.
Raimondo, S., D.N. Vivian, and  M.G. Barren.  2009. Standardizing acute toxicity data for
   use in ecotoxicological models:  influence of test type, life stage, and concentration
   reporting. Ecotoxicology. 18: 918-928.
Shafer, E. W. Jr. and W. A. Bowles Jr. 1985. Acute oral toxicity and repellency of 933
   chemicals to house and deer mice. Arch. Environ. Contam. Toxicol. 14: 111-129.
Shafer, E. W. Jr. and W. A. Bowles Jr. 2004. Toxicity, repellency or phototoxicity of 979
   chemicals to birds, mammals and plants. Research Report No. 04-01. United States
   Department of Agriculture, Fort Collins, CO. 118 p.
Shafer, E. W. Jr., W. A. Bowles Jr. and J. Hurlbut,. 1983. The acute oral toxicity,
   repellency and hazard potential of 998 chemicals to one or more species of wild and
   domestic birds. Arch. Environ. Contam. Toxicol. 12: 355-382.
Smith, G. J. 1987. Pesticide use and toxicology in relation to wildlife: organophosphorus
   and carbamate compounds.  Resource Publication 170. United States Department of
   the Interior, Washington, DC. 171 p.
US Environmental Protection Agency (EPA).  1986. Quality criteria for water. EPA 440/5-
   86-001. Washington,  DC.
US Environmental Protection Agency (EPA).  1996. Ecological Effects Test Guidelines.
   OPPTS 850.1075 Fish Acute Toxicity Test, Freshwater and Marine. EPA 712-C-96-
   118. Washington DC.
US Environmental Protection Agency (EPA). 2005. TREX: Terrestrial Residue
   Exposure model. Office of Pesticide Programs. U.S. Environmental Protection
   Agency.
   http://www.epa.gov/oppefed1/models/terrestrial/trex_usersguide.htm#content4
US Environmental Protection Agency (EPA). 2006. ECOTOX Ecotoxicology Database.
   http://cfpub.epa.gov/ecotox.  Duluth MN.
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                        Appendices
Appendix I. Summary of acceptance requirements for data included in



ICE models
Component
Test organism
Test chemical
Test conditions
Information required
Aquatic taxa tested
Life stage1
Salinity requirements
Test chemical identity
Test chemical purity
Single compound tested
Aqueous exposure
Test duration
Test type
Temperature3
Dissolved oxygen
Salinity3
Acceptance requirements
fish, aquatic invertebrates,
amphibians
species level model: identifiable
to genus and species
genus or family level model:
identifiable to genus or family
juvenile only: fish, amphibians,
insects, mollusks, decapods
all life stages: all other species
identifiable as freshwater (FW)
or saltwater (SW; estuarine or
marine) organism
reported CAS, chemical name
or structure
confirmed name and CAS
>90% or analytical/reagent
grade or equivalent
CAS corresponds to single
compound or element
mixtures excluded except for
chemical salts and specific
congener mixtures
no sediment, dietary or mixed
dose exposures
no phototoxicity results
48 hr: daphnids, midges,
mosquitoes
96 hr: all other species
static, flow-through or static
renewal
species specific (+ 3C)
Test type specific
<1 ppt: FW species
l-5ppt: Cyprinodon bovinus
>15ppt: SW species6
                           24

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Component

Reported toxicity
value
Toxicity value
standardization

Information required
pH or hardness (FW only: required for
specific chemical normalizations)
Acute toxicity endpoint: death (LC50) or
immobilization (EC50)
Concentration units
Concentration units
Chemical specific normalizations'
Element specific normalization7
Acceptance requirements
pH: ammonia,
pentachlorophenol (PCP)
Hardness: Ag, Cu, Cd, Cr(III),
Pb, Ni, Zn
48 hrEC50/LC50: daphnids,
midges, mosquitoes
96 hrEC50/LC50: all other
invertebrates
96 hrLCSO: fish, amphibians
mass/volume or molar units
conversion to ug/L
PCP: pH 6.5
ammonia: pH 8; temperature
dependent
Ag, Cu, Cd, Cr(III), Pb, Ni, Zn:
hardness 50 mg/L
Ag, Al, Cu, Cd, Co, Cr(III),
Cr(VI), Hg, NH4, Ni, Pb, Zn
1 . If life stage not reported, must be determined through reported age/size.
2. Only tests of single compounds; included metal and other chemical salts, and specific
congener mixtures (e.g., standard Aroclors, toxaphene).
3 . Meets ASTM or equivalent test guidelines for test species.
4. Test type specific dissolved oxygen saturation. Static: <48 hr 60-100%; >48 hr 40-100%.
Static renewal or flow-through: 60-100%.
5. FW: test water source identifiable as freshwater, reported salinity <1 ppt, or test species is
a stenohaline freshwater species; only FW salmonid tests.
6. SW: test water identifiable as saltwater, salinity reported to be > 15 ppt, or test species is a
stenohaline saltwater species; only SW striped bass tests were included.
7. Normalized according to AWQC.
25

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Appendix II. List of Species in Aquatic Database
The following species were used to develop Web-ICE aquatic species, genus, or family-
level models.
Invertebrates

Tricladida
      Planariidae  Dugesia tigrina
                                Platyhelminthes
                                   Annelida
Aciculata
      Nereididae  Neanthes virens
Lumbriculida
      Lumbriculidae
                  Lumbriculus variegatus
                                                Flatworm



                                                Polychaete


                                                Polychaete
                                                Short-horned flies

                                                 Midge
                                                 Midge
                                                 Midge
                                    Insecta
Diptera
      Athericidae  Atherix variegata
      Chironomidae
                  Chironomus plumosus
                  Chironomus fen fans
                  Paratanytarsus dissimilis
                  Paratanytarsus parthenogeneticus Midge
Odonata
      Coenagrionidae
                  Ischnura verticalis              Eastern forktail
Plecoptera
      Perlidae     Claassenia sabulosa            Stonefly
      Pteronarcyidae
                  Pteronarcella badia             Stonefly
                  Pteronarcys californica          Stonefly
                                   Crustacea
Diplostraca
      Daphniidae  Ceriodaphnia dubia             Daphnid
                  Daphnia magna                Daphnid
                  Daphnia pulex                 Daphnid
                  Simocephalus serrulatus        Daphnid
Podocopida
      Cyprididae   Cypris subglobosa             Ostracod
Amphipoda
      Crangonyctidae
                  Crangonyx pseudogracilis       Amphipod
                                   26

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      Gammaridae Gammarus fasciatus
      Hyalellidae

Decapoda
      Camba ridae
      Penaeidae

Isopoda
      Asellidae
Mysida
Gammarus lacustris
Gammarus pseudolimnaeus
Allorchestes compressa
Hyalella azteca

Orconectes nais
Farfantepenaeus duorarum
Metapenaeus dobsoni

Asellus aquaticus
Caecidotea brevicauda
Caecidotea intermedia
      Mysidae     Americamysis bahia
Amphipod
Amphipod
Amphipod
Amphipod
Amphipod

Crayfish
Pink shrimp
Kadal shrimp

Isopod
Isopod
Isopod

Mysid
Forcipulatida
      Asteriidae
                                Echinodermata
Aster/as forbesi
                                   Mollusca
Ostreoida
      Ostreidae    Crassostrea virginica
Basommatophora
      Planorbidae  Planorbella trivolvis
Starfish



Eastern oyster

Snail
 Vertebrates
                                    Pisces
Acipenseriformes
      Acipenseridae
                  A cipenser bre virostrum
Atheriniformes
      Atherinopsidae
                  Menidia beryl Una
                  Menidia menidia
Cypriniformes
      Catastomidae
                  Catostomus commersonii
                  Xyrauchen texanus
      Cyprinidae   Carassius auratus
                  Cyprinus carpio
                  Erimonax monachus
                  Gila elegans
                  Notropis mekistocholas
                  Pimephales promelas
                              Shortnose sturgeon
                              Inland silverside
                              Atlantic silverside
                              White sucker
                              Razorback sucker
                              Goldfish
                              Common carp
                              Spotfin chub
                              Bonytail chub
                              Cape fear shiner
                              Fathead minnow
                                  27

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                  Ptychocheilus lucius
Cyprinodontiformes
      Cyprinodontidae
                  Cyprinodon bovinus
                  Cyprinodon variegatus
      Poeciliidae   Gambusia affinis
                  Poecilia reticulata
                  Poeciliopsis occidentalis
Esociformes
      Esocidae    Esox lucius
Gasterosteiformes
      Gasterosteidae
                  Gasterosteus aculeatus
Mugiliformes
      M u g i I i d a e    Che Ion labrosus
Perciformes
      Centrarchidae
                  Lepomis cyanellus
                  Lepomis macrochirus
                  Lepomis microlophus
                  Micropterus dolomieu
                  Micropterus salmoides
                  Pomoxis nigromaculatus
      Channidae   Channa marulius
      Cichlidae
      Percidae
      Sparidae
Salmoniformes
      Salmonidae
Siluriformes
      Ictaluridae
Oreochromis mossambicus
Etheostoma fonticola
Etheostoma lepidum
Perca flavescens
Sander vitreus
Lagodon rhomboides
                  Oncorhynchus clarkii
                  Oncorhynchus gilae
                  Oncorhynchus kisutch
                  Oncorhynchus mykiss
                  Oncorhynchus tshawytscha
                  Sal mo salar
                  Sal mo trutta
                  Salvelinus confluentus
                  Salvelinus fontinalis
                  Salvelinus namaycush
                  Ameiurus met as
                  Ictalurus punctatus
                              Colorado pikeminnow
                              Leon springs pupfish
                              Sheepshead minnow
                              Mosquitofish
                              Guppy
                              Gila topminnow

                              Northern pikeminnow
                              Threespine stickleback

                              Thicklip mullet
Green sunfish
Bluegill
Redear sunfish
Smallmouth bass
Largemouth bass
Black crappie
Bullseye snakehead

Mozambique tilapia
Fountain darter
Greenthroat darter
Yellow perch
Walleye
Pinfish
                              Cutthroat trout
                              Apache trout
                              Coho salmon
                              Rainbow trout
                              Chinook salmon
                              Atlantic salmon
                              Brown trout
                              Bull trout
                              Brook trout
                              Lake trout
                              Black bullhead
                              Channel catfish
                                   28

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                                  Amphibia
Anura
      Bufonidae    Bufo boreas                   Western toad
      Ranidae     Rana sphenocephala           Southern leopard frog
                                  29

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III. List of Species in Wildlife Database
The following species were used to develop Web-ICE wildlife species or family-level
models.
                                    Aves
Anseriformes
      Anatidae
Columbiformes
      Columbidae
                  Anas discors
                  Anas domestica
                  Anas platyrhynchos
                  Anas superciliosa
                  Anas sp.
                  Anas sp.
                  Branta canadensis
                  Dendrocygna bicolor
                  Columba livia
                  Columba oenas
                  Columbina inca
                  Columbina passerina
                  Geopelia cuneata
                  Geopelia humeralis
                  Leptotila verreauxi
                  Streptopelia risoria
                  Streptopelia senegalensis
                  Zenaida asiatica
                  Zenaida auriculata
                  Zenaida macroura
Falconiformes
      Accipitridae
      Falconidae
Galliformes
      Odontophoridae
                  Callipepla californica
                  Callipepla gambelii
                  Colinus  virginianus
      Phasianidae Alectoris chukar
                  Alectoris rufa
                  Centrocercus urophasianus
                  Coturnixjaponica
                  Gal I us gal I us
                  Meleagris gallopa vo
                  Perdix perdix
                  Phasianus colchicus
                  Tympanuchus phasianellus
                  Aquila chrysaetos
                  Falco sparverius
Gruiformes
      Gruidae
                  Grus canadensis
Bluewinged teal
Peking duck
Mallard
Pacific black duck
Pintail
Widgeon
Canada goose
Fulvous whistling duck

Rock dove
Stock dove
Inca dove
Common ground-dove
Diamond dove
Bar-shouldered dove
White-fronted dove
Ringed turtledove
Laughing dove
White-winged dove
Eared dove
Mourning dove

Golden eagle
American kestrel
California quail
Gambel's quail
Northern bobwhite
Chukar
Red partridge
Sage grouse
Japanese quail
Chicken
Turkey
Gray partridge
Ring-necked pheasant
Sharp-tailed grouse

Sandhill crane
                                   30

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Passeriformes
      Corvidae
      Emberizidae
      Fringillidae

      Icteridae
      Passeridae
      Ploceidae
      Sturnidae
      Turdidae
Psittaciformes
      Psittacidae
Strigiformes
Aphelocoma sp.
Corcorax melanorhamphos
Corvus bennetti
Corvus brachyrhynchos
Corvus corax
Corvus coronoides
Corvus frugilegus
Corvus me/for/
Cyanocorax yncas
Pica hudsonia
Pica nuttalli
Junco hyemalis
Spizella pa I I Ida
Volatinia jacarina
Zonotrichia atricapilla
Zonotrichia leucophrys
Carpodacus mexicanus
Serinus sp.
Agelaius phoeniceus
Agelaius tricolor
Euphagus cyanocephalus
Molothrus aeneus
Molothrus ater
Quiscalus major
Quiscalus quiscula
Xanthocephalus xanthocephalus
Neochmia temporal is
Passer domes ticus
Passer luteus
Taeniopygia guttata
Euplectes orix
Ploceus cucullatus
Ploceus taeniopterus
Quelea quelea
Sturnus vulgaris
Turdus migratorius

Aratinga canicularis
Aratinga pertinax
Calyptorhynchus funereus
Melopsittacus undulatus
Myiopsitta monachus
Platycercus elegans
Platycercus eximius
Psephotus haematonotus
Scrub jay
White-winged chough
Little Crow
American crow
Common raven
Australian raven
Rook
Little raven
Green jay
Black-billed magpie
Yellowbilled magpie
Darkeyed junco
Clay-colored sparrow
Blue back grassquit
Golden-crowned sparrow
White-crowned sparrow
House finch
Canary
Red-winged blackbird
Tricolored blackbird
Brewer's blackbird
Bronzed cowbird
Brown-headed cowbird
Boat-tailed grackle
Common grackle
Yellow headed blackbird
Red-browed firetail
House sparrow
Golden sparrow
Zebra finch
Red bishop
Village weaver
Northern masked weaver
Red billed quelea
Starling
American robin

Orange fronted conure
Brown-throated conure
Yellow tailed black cockatoo
Budgerigar
Monk parakeet
Crimson rosella
Eastern  rosella
Red-rumped parrot
                                   31

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      Strigidae    Megascops asio
                              Eastern screech owl
Artiodactyla
      Bovidae

      Cervidae
Carnivora
      Canidae

Lagomorpha
      Leporidae

Rodentia
      Caviidae
      Echimyidae
      Muridae
      Sciuridae
                Mammalia

Capra hircus
Ovis aries
Odocoileus hem/onus

Can is familiaris
Canis latrans

Lepus californicus
Oryctolagus cuniculus

Ca viars poreel I us
Myocastor coy pus
Gerbillus sp.
Microtus californicus
Microtus pinetorum
Microtus sp.
Miscrotus pennsylvanicus
Mus musculus
Oryzomys palustris
Peromyscus maniculatus
Rattus argent/Venter
Rattus exulans
Rattus norvegicus
Rattus rattus
Sigmodon hispidus
Cynomys ludovicianus
Spermophilus beecheyi
Spermophilus lateralis
Spermophilus richardsonii
Domestic goat
Domestic sheep
Mule deer

Dog
Coyote

Blacktailed jackrabbit
Rabbit

Guinea pig
Nutria
Gerbil
Meadow mouse
Pine mouse
Vole
Meadow vole
Mouse
Rice rat
Deer mouse
Ricefield rat
Polynesian rat
Norway rat
Roof rat
Cotton rat
Blacktailed prairie dog
California ground squirrel
Goldenmantled ground squirrel
Richardsons ground squirrel
                                   32

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