&EPA
   MED   in    Review
  www.epa.gov
                        EPA/600/N 07/004
                                                                 Fall 2007
United States Environmental
Protection Agency

•  Office of Research and
  Development

  •  National Health  and
    Environmental Effects
    Research Laboratory

    • Mid-Continent Ecology
     Division, Duluth,
     Minnesota
     In this Issue:

     From the Director

     Research Events

    Featured Research

      Current Events

     New Publications

      MED Seminars

         Awards

         People
   MED in Review Editor
     Mary Ann Starus
      (218)529-5075
 starus.maryann@epa.gov

  MED in Review Design
      FAIR II Contract
     WO68-W-01-032
From the Director, Carl Richards

Welcome to MED in Review. This is the first edition of a new publication designed to
highlight recent and ongoing research activities at the Mid-Continent Ecology Division of the
National Health and Environmental Effects Research Laboratory of ORD.  Our aim is to
provide a snapshot of interesting developments in MED's research portfolio in a friendly
format that both informs on the scope of our activities and encourages more in-depth
communications with our staff. We believe that you will find these editions useful over time
and hope you will give us any thoughtful suggestions on how we might improve the
functionality of this newsletter for your needs. Please feel free to contact us at any time; that's
why we're here.


Research Events
PEER REVIEW OF THE MID-CONTINENT
ECOLOGY DIVISION

On April 17-20, EPA's National Health and
Environmental Effects Research Laboratory
held a peer review of the Division's research
program. A panel of senior scientists was
convened to review the Division's research
programs and accomplishments for the
period 2002-2007. Panel members included
Dr. Tom Burton (Michigan State
University), Dr. Patricia Chow-Fraser
(McMaster University), Dr. Joel Coats (Iowa
State University), Dr. Alan Covich
(University of Georgia), Dr. Peter Hodson
(Queen's University), Dr. Barry Lesht
(Argonne National Laboratory), and
Dr. Charles Pittinger (ARCADIS US, Inc).
During the review week, Division Branch
Chiefs and Senior Scientists provided
overviews of the research conducted under
ORD's multi-year plans. Each briefing also
included a poster session that involved most
of the Division's science staff; over 50
posters were presented. The panel returned
a very helpful and constructive report. The
Division is in the process of providing a
response and series of action items.
Contact: Janet Keough (218) 529-5025.
UNIVERSITY OF MINNESOTA DULUTH
COOPERATIVE TRAINING AGREEMENT

In February, the Division formalized a
cooperative training agreement with the
University of Minnesota after a competitive
proposal process involving several other
large universities. Through this agreement,
EPA will provide funds to the University to
recruit and train both undergraduate and
graduate students as well as postdoctoral
scientists in environmental sciences. The
opportunity to work with senior scientists at
one of EPA's premier environmental
laboratories will enhance the educational
experience of students as they strive to
become leaders within the scientific
community.
                                                                     The University of Minnesota Duluth campus
                                                                                    Continued on next page..
       MED in Review
       Mid-Continent Ecology Division • Duluth, MN

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COOPERATIVE TRAINING AGREEMENT -
CONTINUED

While EPA provides funding and the University
administers the actual recruitment, use of the
funds, and the program overall, EPA scientists will
have a direct involvement with U of M faculty in
selecting successful candidates and providing
mentoring both on and off MED's facilities. Much
of the students' work will take place at MED. This
is yet another tool, like the Student Services
Contract program and summer internships, to train
and mentor future scientists for their careers -
perhaps even in the public service field. MED
scientists are urged to investigate this opportunity
whenever they have the potential to provide funds
toward student training and assistance.

This effort was the brainchild of Dr. Janet Keough
who served as the project writer and coordinator
until proposals were received. At that point, the
Project Officer responsibilities were turned over to
Roger Parsons, who will remain in that capacity,
and can be contacted at (218) 529-5030.
               NORTHLAND CHAPTER OF THE SOT, SPRING MEETING
                             Society of
                             Toxicology
               The spring meeting of the Northland Chapter of the Society of Toxicology
               was held May 3 in MED's conference center. Titled "Down the Drain and
               Out of Our Minds," the meeting focused on contemporary issues in
               wastewater management, with an emphasis on Pharmaceuticals and "personal
               care" products.  Invited speakers gave four presentations:

                   Medaka: A good fish model for assessing hazards associated -with
                   waterborne contaminants: Dr. Rodney Johnson, EPA/MED;

                   The evolving landscape of-waste-water regulation over the last 35 years:
                   Tim Scherkenbach, Minnesota Pollution Control Agency;

                   Photochemical cycling oftriclosan: Degradation and the formation of
                   toxic products: Dr. Doug Latch, Seattle University;

                   Exposures, effects,  and risks of agricultural pharmaceuticals in surface
                   •waters and soil: Dr. Keith Solomon, University of Guelph.

               Contact: John Nichols  (218) 529-5160.
INTERNATIONAL SCIENCE FORUM ON COMPUTATIONAL TOXICOLOGY
 UNITED STATES ENVIRONMENTAL PROTECTION AQfcNGY
Two MED researchers participated
in the Forum, held at EPA's Main
Campus in Research Triangle Park,
North Carolina, on May 21-23.
Designed for public, private sector,
and academic researchers active in
the field as well as risk assessors
and risk managers, the Forum
highlighted progress in the  use of
modern molecular, biological, and
chemical based tools on the
assessment of exposure, hazard,
and risk of environmental
chemicals. Approximately 50
invited speakers from around the
world presented the latest advances
in computational toxicology.
Plenary sessions led by prominent
keynote speakers examined
development of virtual tissues and
organs, models of ecosystem
function, and gene-environment
interactions. Dan Villeneuve of
            MED's Toxic Effects
            Characterization
            Research Branch co-
            chaired the plenary
session "Computational Tools for
Ecological Risk Assessment." He
also presented Mining minnows and
building models: An integrated
systems biology approach to link
mechanism of action to
ecologically-relevant outcomes.
Matthew Etterson, National
Research Council Post-Doc at
MED, presented Toxicology versus
ecology in population-level risk
assessment for-wildlife: What data
does your modeler really need?
Concurrent sessions covering
related topics followed two tracks -
computational models, and tools for
hazard prediction. An evening
poster session featured contributed
research focused on similar themes.
Contacts: Matt Etterson
(218)529-5158 and
Dan Villeneuve (218) 529-5217.
ORD SCIENTISTS PARTICIPATE IN FISH
TOXICOGENOMICS WORKSHOP

The UK National Environment Research Council
(NERC), through the University of Birmingham,
sponsored a workshop entitled "Fish
Toxicogenomics: Advancing Practical
Implementation" May 24-26 in Aveiro, Portugal.
Invited scientists from around the world met to
identify needs and develop experimental projects to
focus NERC funding to support the application of
genomics to ecological risk assessment.  Specific
topical areas included bioinformatics,
transcriptomics, metabolomics, and data
interpretation (toxic  vs. compensatory responses).
Three ORD scientists, Drs. Gerald Ankley
(NHEERL/MED), Tim Collette (NERL/ERD) and
William H. Benson (NHEERL/GED and Office of
the Science Advisor) were among the
approximately 40 invitees, as were two scientists
receiving funding from EPA, via the STAR
program - Dr. Nancy Denslow (University of
Florida) and via an interagency agreement - Dr.
Ann Miracle (Pacific Northwest Laboratories,
DOE). Contacts:  Gerald Ankley (218) 529-5147,
Tim Collette (706) 355-8211, or William Benson
(850)934-9208.
          MED in Review
          Mid-Continent Ecology Division • Duluth, MN

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VISITING EXPERT FROM UNIVERSITY OF UTRECHT

For three months this summer, Division researchers learned a Solid Phase
Microextraction (SPME) technique from Dr. Thomas ter Laak, a scientist
from the University of Utrecht, the Netherlands. SPME measures the free
concentration of a compound in an experimental system, and can be
applied to solutions, tissues, sediments, and other matrices. The resulting
information can be used to estimate sorption coefficients and may
contribute to the interpretation of in vitro and in vivo test data.  Specific
applications of the SPME method that are being pursued include the
effect of chemical binding on hepatic metabolism in an isolated perfused
fish liver preparation and determination of free chemical concentrations
in an in vitro estrogen receptor binding assay.
Contact: John Nichols (218) 529-5160.
PELLSTON WORKSHOP

EPA scientists from NHEERL (Russ Erickson and Dave Mount, MED
and Mace Barren, GED) and OPP (Keith Sappington, EFED)
participated in a Pellston Workshop of the Society of Environmental
Toxicology on the "Tissue Residue Approach to Ecotoxicity
Assessment." The workshop, held June 7-12 in Leaven worth,
Washington, included invited experts in ecotoxicology and environmental
chemistry from North America, Europe, and Asia. Participants examined
the theory and practice of using organism-based dose metrics (e.g.,
whole-body, organ, tissues) to assess ecological risks, and to
conceptualize methods to translate residue-response data into regulatory
guidelines for tissue,  sediment, and water quality.  Individual working
groups focused on scientific underpinnings of the approach,
mixtures/mechanisms, metals and metal compounds, organic compounds,
and application to risk assessment. Contacts: Russ Erickson
(218) 529-5157 and Dave Mount (218) 529-5169.
                                       MED HOSTS DISCUSSIONS ON WATER QUALITY
                                       CRITERIA FOR EDCs

                                                                        On July 23-26,
                                                                        MED hosted
                                                                        meetings with
                                                                        managers and
                                                                        staff from the
                                                                        Office of Water's
                                                                        Office of Science
                                       and Technology (OW/OST). On July 23, senior
                                       managers from OW/OST met with MED senior staff
                                       to discuss research across all GPRA goals with
                                       relevance to OW science priorities, including
                                       endocrine disruption, Pharmaceuticals and other
                                       emerging contaminants, techniques for toxicological
                                       extrapolation across species and chemicals, "omics"
                                       techniques, biological condition assessment and
                                       diagnosis, and nutrient and habitat effects on
                                       ecological condition.  On July 24-26, there were
                                       technical meetings of OW/OST and ORD scientists
                                       which focused on developing supplemental methods
                                       and guidance for deriving water quality criteria
                                       (WQC) for endocrine-disrupting compounds (EDCs)
                                       and other chemicals with properties that are more
                                       difficult to properly assess using existing WQC
                                       derivation approaches. This meeting is a step in a
                                       larger ongoing technical assistance effort.  Scientists
                                       from NERL-Cincinnati specializing in detection and
                                       analysis of EDC chemicals are also part of the
                                       workgroup and attended this meeting. Division
                                       attendees were Gary Ankley, Rick Bennett,
                                       Russ Erickson, Dale Hoff, Dave Mount,
                                       Chris Russom, Chuck Stephan, and Joe  Tietge.
                                       Contact: Dale Hoff (218) 529-5386.
Rod Booth (MED Facilities Mgr.), Carl
Richards (MED Director), & Hal Zenick
NHEERL DIRECTOR, DR. HAL ZENICK, VISITS THE DIVISION

This August 27 - 28 visit proved to be an excellent opportunity to brief Dr. Zenick on MED's
research programs, since he had been unable to attend our peer review in April. We always
appreciate visits from the NHEERL  "Mother Ship," and Hal shared his perspectives on
programmatic futures within the Laboratory and ORD. The Division held a "potluck and brats"
luncheon, followed by an all-hands briefing by Dr. Zenick and discussion with the staff. Several
awards were presented.
2007 McKiM CONFERENCE ON PREDICTIVE TOXICOLOGY

The Division participated in the "McKim Conference on
Predictive Toxicology" held in Duluth on September 25-27'.
The Conference was organized by the International QSAR
Foundation and convened about 80 scientists to develop the
science needed for hypothesis-driven testing strategies. The
Conference was inspired by and named in honor of the late
James M. McKim III, long-time MED employee, whose
pioneering contributions in the field of aquatic toxicology
                                          International

                                   The McKim Conferences-

                             provided the science necessary to develop sound ecological risk
                             assessment principles. The McKim Conference is not a forum
                             for the presentation of individual scientific research, but rather a
                             forum for discussion of specific issues which are framed by
                             invited speakers.
                                                                   Continued next page..
          MED in Review
          Mid-Continent Ecology Division • Duluth, MN

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McKiM CONFERENCE - CONTINUED

Last year, the McKim Conference discussed the paradigm shift
occurring in numerous regulatory agencies, and outlined three
major areas where science currently limits a more strategic
paradigm in safety assessment. The 2006 conferees urged that
future meetings be focused on specific gaps in predictive
toxicology and chemistry that limited extrapolation of existing
data across chemicals, species, and test endpoints. Important
gaps included the models and dose metrics  used for chronic
non-cancer endpoints including reproductive and developmental
impairment. Accordingly, the organizers of the 2007 Conference
invited speakers who gave thought-starter papers in systems
biology for the brain-pituitary-gonad axis and illustrated how
these studies guide the development of specific reproductive
models for different toxicity pathways. The conferees discussed
methods  of incorporating these models into different chemical
categories to fill data gaps within specific regulatory domains.

Conference topics:
• Chemical Categories and Predictive Toxicology
• New Reference Points for Hazard Identification
• Using  Systems Approaches to Strategic Testing
•  Special QSAR Toolbox Training Session
  The QSAR Application Toolbox -was developed by OECD and
  the Laboratory of Mathematical Chemistry to facilitate the use
  of chemical categories and QSAR in the international hazard
  assessment of chemicals.

ORD presentations made at the conference:
Bioinformatics and systems biology - Imran Shah/NCCT
Predicting estrogen receptor binding within categories -
  Rick Kolanczyk/MED
Dose-response for neurological effects in narcosis -
  Phil Bushnell/NTD
Dose-response for reproductive effects of ER binders -
  Pat Schmieder/MED
Systems biology in small fish models - Dan Villeneuve/MED
Bridging from individuals to populations - Matt Etterson/MED
Systems biology in amphibian models - John Nichols/MED
Opportunities for strategic planning using systems models -
  Gary Ankley/MED
Defining reproductive or neurological hazards -
  Pat Schmieder/MED, Phil Bushnell/NTD

Contact: Pat Schmieder (218) 529-5161.
MED SCIENTIST WORKING WITH NHEERL/ETD, REGION 8, AND OSWER TO SUPPORT EPA's LIBBY ACTION PLAN
                      NHEERL and OSWER representatives were invited to Libby, Montana, for meetings on September 26-27', to
                      discuss research addressing the health effects of asbestos contamination in that community. Vermiculite ore
                      mined for 70 years near the town contained substantial amounts of amphibole asbestos and related mineral
                      fibers.  A research plan written by NHEERL with the assistance of EPA Region 8, ORD NCEA, and OSWER
                      was developed to improve the scientific basis for the risk assessment in Libby, and is available on the
                      Region 8 website at http://www.epa.gov/region8/superfund/libby/ amphibole.html.

At the meeting in Libby, Doug Ammon (Chief, Science Policy Branch, Office of Superfund Remediation and Technology Innovation,
OSWER)  described the role of OSWER. Dr. Linda Birnbaum (Experimental Toxicology Division Director) provided an overview of
NHEERL and its capabilities. Dr. Phil Cook/MED described the NHEERL historical asbestos research database and plans for in vitro
fiber dissolution studies. Dr. Stephen Gavett/ETD discussed plans for in vitro toxicity studies and subchronic inhalation exposure
studies in rats.  Dr. Urmila Kodavanti/ETD discussed plans for comparative toxicity and intratracheal instillation studies in rodents.
Annie Jarabek (ORD/NCEA, on detail to NHEERL/ETD) described plans for developing a dosimetry model and its relevance for risk
assessment methodology. Participants also toured the remediation sites, had discussions on future research plans, and attended a
public session to discuss how the science will benefit the public. Contact: Dr. Stephen Gavett (919) 541-2555.
Featured Research
MED AND EPA REGION 5 PARTNERSHIP IN RESEARCH AND REMEDIATION CULMINATES IN TRENTON, MICHIGAN

On June 18, the first Great Lakes Legacy Act remediation site, the Black Lagoon in Trenton,
Michigan, was re-dedicated as Ellias Cove, named after the donor of the adjacent land known as
Meyer-Ellias Park.  At the ribbon-cutting on the restored shoreline, an announcement was made that
a long-awaited marina would be developed at the site. The City of Trenton hosted the event with
numerous partners:  participating were high-level officials from EPA, represented by
Dr. Carl Richards, MED Director; US Fish and Wildlife Service (USFWS); US Army Corps of
Engineers (Corps);  the Michigan Departments of Environmental Quality (MDEQ) and Natural
Resources; and many political dignitaries.
                                                                   Continued on next page...
          MED in Review
          Mid-Continent Ecology Division • Duluth, MN

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ELLIAS COVE, TRENTON, MICHIGAN -
CONTINUED

Other key project partners are:
Metropolitan Affairs Coalition's Greater
Detroit American Heritage River
Initiative, Michigan Sea Grant, Detroit
River Remedial Action Plan, JJR, the
Ellias family, and the Great Lakes Basin
Program for Soil Erosion and Sediment
Control.

The two-acre Black Lagoon lies within
the USFWS Detroit River International
Wildlife Refuge, the first of its kind in
North America. MED, Region 5, and
their partners have had a long history at
this site. In the mid 1980s, EPA's ORD
staff in Grosse He, Michigan (now part of
MED) conducted the primary assessment
of the site under the Upper Great Lakes
Connecting Channels Study and the
Detroit River In-Place Pollutants Study.
A  series of toxicity and mutagenicity
tests, benthic invertebrate investigations,
chemical analyses, and mathematical
modeling were conducted. The lagoon
was characterized as one of the most
severely contaminated (by PCBs,
mercury, oil, grease, lead, and zinc) and
impacted sites in the Detroit River and
for all of the Great Lakes. (The name
"Black Lagoon" was coined because
extremely contaminated black sediments
were trapped in this backwater
embayment as they moved downstream
from Detroit-area industries.)
Subsequently, a series of other
characterizations were completed.

In 2004-2005, Region 5's Great Lakes
National Program Office (GLNPO), the
EPA Office of Solid Waste and
Emergency Response, MDEQ, and the
Corps completed dredging at the site
under the Great Lakes Legacy Act. In
total, $9.3 million - EPA 65%, MDEQ
35% - was spent to remove 115,000
cubic yards of sediment and dispose of it
in a special containment cell at the Pointe
Mouillee Confined Disposal Facility.
Since that time, the City of Trenton and
USFWS, in cooperation with the Detroit
River International Wildlife Refuge, have
completed the shoreline restoration
project. The USFWS awarded funds
through the Boating Infrastructure  Grant
to the City of Trenton with an appropriate
match, to begin construction of a
much-needed marina to stimulate the
local economy.

Shoreline changes at the cove include
new depth where the sediment was
removed, and boulder and native plant
installation to provide three types of new
habitat for various returning wildlife. At
least seven pairs of bald eagles nest near
the wildlife refuge. Ospreys and peregrine
falcons also enjoy the area, as in the days
before contamination. Fish expected to
return and thrive are whitefish, sturgeon,
salmon, yellowbelly perch, and walleye.

This project is an excellent example of
research, modeling, and remediation
coming together to help restore a portion
of the Detroit River, a region that is
becoming internationally known for its
public-private partnerships for outdoor
recreation, conservation, and quality of
life. The river is one of only 14 in the US
to be honored with an American Heritage
River designation. In 2001, it also
received a Canadian Heritage River
designation, making it the first
international heritage river system in
North America.

This landmark restoration marks a
significant demonstration of the way a
sound science and research foundation
can lead to restoration of ecological
services and economic vitality.
Contact: Russell G. Kreis Jr. (734)
692-7615.
Current  Events
MAKING A GREAT LAKE SUPERIOR, OCT. 29-31
                         Organized by the Lake Superior Binational Program and a large number of federal, state, tribal,
                         educational, and other organizations within the Lake Superior Basin, "Making a Great Lake Superior" is a
                         conference on Lake Superior science, management, and education. The conference is being held at the
                         Duluth Entertainment and Convention Center, and features presentations on the science, management, and
                         education of topics such as invasive species, human health, Areas of Concern, sources and types of
                         pollution, economic and environmental sustainability, climate change, water level change, watershed
                         stewardship, and fisheries and aquatic ecology. The conference features many "green" practices, such as
                         mass transportation, use of recycled materials, energy conservation, and volunteer activities.
                         Dr. Carl Richards, MED Director, is giving one of the keynote addresses in a session on "Past and Future
                         States of Lake Superior."  Several MED scientists are making oral and poster presentations. For more
                         information, go to: http://www.seagrant.umn.edu/superior2007/.
          MED in Review
          Mid-Continent Ecology Division • Duluth, MN

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New  Publications since 3/13/2007
Albers, P.H., M.T. Koterba, R. Rossmann, W.A. Link, J.B. French,
  R.S. Bennett, and W.C. Bauer. 2007. Effects of methyl mercury
  on reproduction in American kestrels. Environmental
  Toxicology and Chemistry 26:1856-1866.

Ankley, G.T., K.M. Jensen, M.D. Kahl, E.A. Makynen, L.S. Blake,
  K.J. Greene, R.D. Johnson, and D.L. Villeneuve. 2007.
  Ketoconazole in the fathead minnow (Pimephalespromelas)'.
  Reproductive toxicity and biological compensation.
  Environmental Toxicology andChemistry 26:1214-1223.

Blystone, C.R., C.S. Lambright, K.L. Howdeshell, J. Furr,
  R.M. Sternberg, B.C. Butterworth, E.J. Durhan, E.A. Makynen,
  G.T. Ankley, V.S. Wilson, G.A. LeBlanc, and J.L.E. Gray.
  2007. Sensitivity of fetal rat testicular steroidogenesis to
  maternal prochloraz exposure and the underlying mechanism of
  inhibition. ToxicologicalSciences 97:512-519.
Brazner, J.C., N. Danz, G.J. Niemi, R.R. Regal, A. Trebitz,
  R. Howe, J. Hanowski, L. Johnson, J. Ciborowski, C. Johnson,
  E. Reavie, V.J. Brady, and G. Sgro. 2007. Evaluation of
  geographic, geomorphic and human influences on Great Lakes
  wetland indicators:  A multi-assemblage approach. Ecological
  Indicators 7:610-635.

Breen, M.S., D.L. Villeneuve, M. Breen, G.T. Ankley, and
  R.B. Conolly. 2007. Mechanistic computational model of
  ovarian steroidogenesis to predict biochemical responses to
  endocrine active compounds. Annals ofBiomedical
  Engineering 35:970-981.

Danz, N., G.J. Niemi, R.R. Regal, T. Hollenhorst, L. Johnson,
  J. Hanowski, R.P. Axler, J.  Ciborowski, T. Hrabik,  V.J. Brady,
  J.R. Kelly, J.A. Morrice, J.C. Brazner, R. Howe, C.A. Johnson,
  and G.E. Host. 2007. Integrated measures of anthropogenic
  stress in the U.S. Great Lakes Basin. Environmental
  Management 39:631-647.

Ekman, D., Q. Teng, K. Jensen, D. Martinovic, D. Villeneuve,
  G. Ankley, and T. Collette.  2007. NMR analysis of male fathead
  minnow urinary metabolites: A potential approach for studying
  impacts of chemical exposures. Aquatic Toxicology
  85:104-112.

Erickson, R.J. 2007. Quantification of toxic effects for water
  concentration-based aquatic life criteria - Part A. U.S.
  Environmental Protection  Agency, EPA/600/R-07/065.
Etterson, M.A., J.R. Etterson, and F. J. Cuthbert. 2007. A robust
  new method for analyzing community change and an example
  using  83 years of avian response to  forest succession.
  Biological Conservation 138:381-389.

Etterson, M.A., L.R. Nagy, and T.R. Robinson. 2007.  Partitioning
  risk among different causes of nest failure. The Auk
  124:432-443.

Etterson, M.A., B. Olsen, and R. Greenberg. 2007. The analysis of
  covariates in multi-fate Markov chain nest failure models.
  Studies in Avian Biology 34:55-64.

Fitzsimmons, P.N., G.J. Lien, and J.W. Nichols. 2007. A
  compilation of in vitro rate  and affinity values for xenobiotic
  biotransformation in fish, measured under physiological
  conditions. Comparative Biochemistry and Physiology Part C
  145:485-506.
Helbing, C.C., C.M. Bailey, L. Ji, M. Gunderson, F. Zhang,
  N. Veldhoen, R.C. Skirrow, R. Mu, M. Lesperance,
  G.W. Holcombe, P.A. Kosian, J.E. Tietge, J.J. Korte, and
  S.J. Degitz. 2007. Identification of gene expression indicators
  for thyroid axis disruption in aXenopus laevis metamorphosis
  screening assay. Part 1. Effects on the brain. Aquatic
  Toxicology 82:227-241.

Helbing, C.C., L. Ji, C.M. Bailey, N. Veldhoen,  F. Zhang,
  G.W. Holcombe, P.A. Kosian, J.E. Tietge, J.J. Korte, and
  S.J. Degitz. 2007. Identification of gene expression indicators
  for thyroid axis disruption in aXenopus laevis metamorphosis
  screening assay.  Part 2. Effects on the tail and hindlimb.
  Aquatic Toxicology 82:215-226.

Hoffman, J.C., D.A. Bronk, and J.E. Olney. 2007. Tracking
  nursery habitat use in the York River estuary, Virginia by young
  American shad using stable isotopes. Transactions of the
  American Fisheries Society 136:1285-1297.

Larkin, P., D.L. Villeneuve, I. Knoebl, A.L. Miracle, B.J. Carter,
  L. Liu, N.D. Denslow, and G.T. Ankley. 2007. Development and
  validation of a 2,000 gene microarray in the fathead minnow
  (Pimephales promelas). Environmental Toxicology and
  Chemistry 26:1497-1506.

Nichols, J.W., P.N. Fitzsimmons, and L.P. Burkhard. 2007. In
  vitro-in vivo extrapolation of quantitative hepatic
  biotransformation data for fish.  II. Modeled effects on chemical
  bioaccumulation. Environmental Toxicology and Chemistry
  26:1304-1319.

Smith, P.N., G.P. Cobb, C. Godard-Codding, D. Hoff,
  S.T. McMurry, T.R. Rainwater, and  K.D. Reynolds.
  Contaminant exposure in terrestrial vertebrates. 2007.
  Environmental Pollution doi:W.W16/j.envpo\.2007.06.009.

Trebitz, A.S., J.C. Brazner, V.J. Brady, R.P. Axler, and
  D.K. Tanner. 2007. Turbidity tolerances of Great Lakes coastal
  wetland fishes. North American Journal of Fisheries
  Management 27:619-633.

Villeneuve, D.L., G.T. Ankley, E.A. Makynen, L.S. Blake,
  K.J. Greene, E.B. Higley, J.L. Newsted, J.P. Giesy, and
  M. Hecker. 2007. Comparison of fathead minnow ovary explant
  and H295R cell-based steroidogenesis assays for identifying
  endocrine-active chemicals. Ecotoxicology and Environmental
  Safety 68:20-32.
Villeneuve, D.L., L.S. Blake, J.D. Brodin, K.J. Greene, I. Knoebl,
  A.L. Miracle, D. Martinovic, and G.T.  Ankley. 2007.
  Transcription of key genes regulating gonadal steroidogenesis in
  control and ketoconazole- or vinclozolin-exposed fathead
  minnows.  Toxicological Sciences 98:395-407.

Watanabe, K.H., K.M. Jensen, E.F. Orlando, and G.T. Ankley.
  2007. What is normal? A characterization of the values and
  variability in reproductive endpoints of the fathead minnow,
  Pimephales promelas.  Comparative Biochemistry and
  Physiology Part C 146:348-356.
          MED in Review
          Mid-Continent Ecology Division • Duluth, MN

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MED Seminars
RECENT AND UPCOMING SEMINARS
Aug. 27
Ross Lunetta, EPA/National Exposure
Research Lab Landscape Branch
• Landscape change detection and
  characterization project for the Great
  Lakes basin

Sept. 4
Stephen Edwards, EPA National Health
and Environmental Effects Research Lab
• Causal gene networks and systems
  biology at the EPA
Sept. 12
Marinus L. Otte, North Dakota State
University
• Biogeochemistry and metal tolerance in
  wetland plants - Implications for the
  evolution of metal tolerance in all
  plants

Oct. 3
Dr. Jay Austin, UMD Large Lakes
Observatory
• Climate change signals from Lake
  Superior
Oct. 17
Allen Olmstead, MED
• Xenopus Tropicalis reproductive
  toxicology

Dec. 12
• MED Division-wide poster event

Feb. 6
David Miller, MED
• Lower food web ecosystem
  productivity modeling applied to Lake
  Michigan
Awards
EPA NATIONAL HONOR AWARD
   Sulfate Criteria Team, Region 5 - Charles E. Stephan (and Region 5/OW recipients)
      For protecting aquatic life by exemplary application of sound science and effective multi-party collaboration to develop and
      apply sulfate -water quality criteria for Illinois.

ORD 2006 BRONZE MEDALS
   Christine L. Russom
      For innovation -within the ECOTOX Knowledge System to improve access and utility of high quality chemical toxicity
      information for risk assessment and research.
   Richard S.  Bennett
      Recognizing exceptional efforts to provide consultation and guidance to EPA Program and Regional Offices in the area of
      •wildlife risk assessment and population modeling techniques.
   Anne M. Cotter, Colleen M. Elonen, Brian H. Hill, Terri M. Jicha, John R. Kelly, Michael L. Knuth, John A.  Morrice,
   Gregory S. Peterson, Michael E. Sierszen, Debra L. Taylor, Jo A. Thompson, Anett S. Trebitz, and Corlis W.  West (and
   other members of the Nutrients Research Team)
      For scientific leadership in research to improve technical guidance for establishing nutrient criteria for estuarine, coastal, and
      Great Lakes -waters.
   Teresa J. Norberg-King (and other members of the ORD Microtox Team)
      For exceptional technical assistance efforts by the Microtox Team to  ensure that EPA 's -whole effluent toxicity test methods are
      appropriately validated.

EPA's SUSTAINABLE FACILITIES PRACTICES  BRANCH AWARDS
   BTU Buster Award  Rodney H. Booth, MED Facilities Manager
      In  recognition of reduction in energy use by more than 12.5 % in FY  2006, through  diligent management of laboratory
      operations, including manually resetting cooling and heating set points on the facility's building automation system, as -well as
      completing a variable air volume system installation.

   First Annual Green Thumb Award - Landscaping Team: Rodney H. Booth, Alex D. Hoffman, Janet R. Keough,
   Patricia A.  Kosian, Elizabeth A. Makynen, Christine L. Russom, Mary Ann Starus, Debra L. Taylor, and Corlis W. West
      For the implementation of on site sustainable landscaping, by planting native grasses,
      wild/lowers, and shrubs, reducing mo-wing and -water runoff. Team members also started a
      public education campaign, creating an insert for the visitor brochure and a poster in the
      building lobby that explain the landscaping project.
         MED in Review
         Mid-Continent Ecology Division • Duluth, MN

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NHEERL QUALITY ASSURANCE AWARD
   Anne M. Cotter
      In recognition of the implementation of software for sample tracking using bar codes.

NHEERL GOAL 1 AWARD: SUPPORT THE AGENCY'S MISSION
   Brian H. Hill, Mary F. Moffett (and other recipients in GED/AED/WED)
      In recognition of outstanding efforts in the research and operational support for aquatic monitoring programs.

REGIONAL SETAC BEST POSTER AWARD
   Lindsey Blake
   Blake, L.S., D.L. Villeneuve, K.M. Jensen, D. Martinovic, M.D. Kahl, J.D. Brodin, E.A. Makynen, E.J. Durban, and G.T. Ankley.
   Effects of trilostane on the fathead minnow (Pimephales promelas).
      Lindsey Blake, former MED Student Contractor Services (SSC), -won the best student poster award at the Mid-west regional
      SETAC meeting, March 14-16, at Argonne National Laboratory in Chicago. She presented some of the gene expression -work
      she has done on the Small Fish CT project. This -was a joint meeting -with the Chicago Chapter of the Society for Risk Analysis,
      so there -was added competition. The award covers the student's travel costs to the national SETAC meeting in Milwaukee in
      November. Lindsey is now enrolled in graduate studies at the University of Minnesota Duluth, and Gary Ankley/MED is her
      advisor.
People
WELCOME PAULINE!
FRANK MOVES TO HEALTH AND SAFETY
                       In June, Pauline Davies joined us as a
                       Senior Environmental Employee
                       (SEE) receptionist.  She is a Duluth
                       native, and her family includes two
                       daughters and two Bichons. Pauline
                       has experience in publishing, and
                       owns a  small manufacturing business.
                       She plays the violin, cross country
                       skis at her cabin, and is a fan of UMD
                       men's hockey.
GREG LIEN AND RITA MARROW RETIRE
                        After a long and illustrious career,
                        starting in 1978, Aquatic Biologist
                        Greg Lien retired on April 30.
After about 20 years in the Air
Force, Rita worked at MED as
General Supply Specialist since
1998, and retired July 21.
                        Frank Puglisi, retired MED
                        Analytical/Research Chemist, is the
                        new SEE Occupational Safety and
                        Health technician. Frank will also
                        work with MED researchers on
                        pesticide metabolism, and will
                        become another expert on our
                        chemical inventory database, with
                        monitor Eric Mead,  SHEM Program
                        Manager.
CRAIG JOHNSON AND MARIE NICHOLS-JOHNSON MOVE TO
NCEA/ClNCINNATI
                          Craig Johnson was our Acquisition
                          Manager since 1994. He has taken
                          a position at the EPA/ORD
                          National Center for Environmental
                        i  Assessment (NCEA), as a Senior
                          Program Analyst and Quality
                          Assurance Coordinator.
Marie Nichols-Johnson came to
MED in 2001, and served as
Program Analyst. She is now a
Contract Support Specialist at
NCEA.
/
         MED in Review
         Mid-Continent Ecology Division • Duluth, MN

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