EPA/600/R-09/074 June 2010
United States
Environmental Protection
Agency
Sample Collection Information Document
Companion to Standardized Analytical Methods for
Environmental Restoration Following Homeland
Security Events (SAM) - Revision 5.0
Office of Research and Development
National Homeland Security Research Center
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Sample Collection Information
Document
Companion to Standardized Analytical
Methods for Environmental Restoration
Following Homeland Security Events
(SAM) - Revision 5.0
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
Office of Research and Development, National Homeland Security Research Center,
Cincinnati, OH 45268
Office of Research and Development
National Homeland Security Research Center, Threat and Consequence Assessment Division
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Acknowledgements
This document was developed by the U.S. Environmental Protection Agency's (EPA) National Homeland
Security Research Center (NHSRC) within EPA's Office of Research and Development as a companion
to NHSRC's Standardized Analytical Methods for Environmental Restoration Following Homeland
Security Events (SAM). We wish to acknowledge the external peer reviews conducted by Matthew
Arduino of the U.S. Centers for Disease Control and Prevention (CDC), Larry Burchfield of the
Radiochemistry Society, Jordan Peccia of Yale University, and Fred Lee of G. Fred Lee & Associates,
whose thoughtful comments contributed greatly to the quality of the information. The document was
prepared by Computer Sciences Corporation (CSC) under EPA Contract No. EP-W-06-046.
Disclaimer
Mention of trade names or commercial products in this document does not constitute endorsement or
recommendation for use.
Questions concerning this document or its application should be addressed to:
Romy Lee
National Homeland Security Research Center
Office of Research and Development (NG16)
U.S. Environmental Protection Agency
26 West Martin Luther King Drive
Cincinnati, OH 45268
(513)569-7016
lee.romy@epa.gov
June 2010
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Foreword
Following the events of September 11, 2001, EPA's mission was expanded to account for critical needs
related to homeland security. Presidential Directives identified EPA as the primary federal agency
responsible for the country's water supplies and for decontamination following a chemical, biological,
and/or radiological (CBR) attack. To provide scientific and technical support to help EPA meet this
expanded role, EPA's National Homeland Security Research Center (NHSRC) was established. The
NHSRC research program is focused on conducting research and delivering products that improve the
capability of the Agency to carry out its homeland security responsibilities.
One specific focus area of NHSRC's research is to support the Environmental Response Laboratory
Network (ERLN), a nationwide association of federal, state, local, and commercial environmental
laboratories, established by EPA. The ERLN can be deployed in response to a large-scale environmental
disaster by providing consistent analytical capabilities, capacities, and quality data in a systematic,
coordinated manner. Toward this end, NHSRC has worked with experts from across EPA and other
federal agencies to develop a compendium of analytical methods to be used in support of remediation
following national homeland security related incidents. For specific analytes that have been determined
to be of concern during a homeland security related event, analytical methods have been chosen to
measure levels of contamination in different environmental matrices. The results of these efforts have
been published in EPA's Standardized Analytical Methods for Environmental Restoration Following
Homeland Security Events (SAM), available at http://www.epa.gov/sam.
In identifying and selecting appropriate analytical methods to be used in such instances, the need to
provide information regarding the collection of samples for analysis by these agreed upon methods
became apparent. The information in this document addresses this need, in part, by providing
recommendations on appropriate sample containers, packaging, preservation, and sources for
additional information supporting sample collection procedures. Updates will be provided as new
information is developed regarding sampling or analytical procedures.
NHSRC has made this publication available to assist in preparing for and recovering from disasters
involving chemical, radiochemical, and biological contamination; it specifically represents an important
next step in supporting the ERLN. We value your comments as we move toward the development of an
efficient process to manage environmental samples and move EPA one step closer to achieving its
homeland security mission and its overall mission of protecting human health and the environment while
supporting sustainable solutions.
Cynthia Sonich-Mullin, Acting Director
National Homeland Security Research Center
June 2010
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Table of Contents
ACKNOWLEDGEMENTS i
LIST OF ATTACHMENTS iii
ACRONYMS AND ABBREVIATIONS iv
1.0 BACKGROUND 1
2.0 SCOPE AND APPLICATION 1
2.1 SAMPLE COLLECTION INFORMATION TABLES 2
2.2 DOCUMENT DEVELOPMENT. 2
2.3 LIMITATIONS 3
3.0 HEALTH AND SAFETY CONSIDERATIONS 4
3.1 HEALTH AND SAFETY PLANS 4
3.2 PERSONAL PROTECTIVE EQUIPMENT 4
3.3 TRAINING 5
4.0 PREPARATION FOR SAMPLE COLLECTION 5
4.1 FIELD SAMPLING EQUIPMENT AND SUPPLIES 5
4.2 FIELD DATA DOCUMENTATION 5
5.0 PREPARATION OF SAMPLE CONTAINERS 6
5.1 SAMPLE CONTAINER LABELS 6
5.2 PREPARING SAMPLE CONTAINERS FOR PACKAGING 6
5.3 GENERAL SAMPLE SHIPMENT GUIDELINES 6
5.4 CHAIN-OF-CUSTODY FORMS 6
5.5 CUSTODY SEALS/TAMPER-EVIDENT BAGS 7
6.0 DEFINITIONS 7
7.0 REFERENCES 8
List of Attachments
Attachment A: Sample Collection Information for the Chemical Analytes Listed in SAM
Attachment B: Sample Collection Information for the Radiochemical Analytes Listed in SAM
Attachment C: Sample Collection Information for the Pathogens Listed in SAM
Attachment D: Sample Collection Information for the Biotoxin Analytes Listed in SAM
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Acronyms and Abbreviations
AOAC Association of Official Analytical Chemists
ASTM American Society for Testing and Materials
AWWA American Water Works Association
CDC Centers for Disease Control and Prevention
CFR Code of Federal Regulations
Ch. Chapter
CLP Contract Laboratory Program
COC Chain-Of-Custody
CSC Computer Sciences Corporation
°C Degrees Celcius
DGR Dangerous Goods Regulations
DHS U.S. Department of Homeland Security
DIMP Diisopropyl methylphosphonate
DOE U.S. Department of Energy
DOL U.S. Department of Labor
DOT U.S. Department of Transportation
EA2192 Diisopropylaminoethyl methyl thiolophosphonate
ED Ethyldichloroarsine
EDEA N-Ethyldiethanolamine
EMPA Ethylmethyl phosphonate
EMSL Environmental Monitoring and Support Laboratory
EPA U.S. Environmental Protection Agency
FDA U.S. Food and Drug Administration
FRMAC Federal Radiological Monitoring and Assessment Center
g gram(s)
gal Gallon
GC Gas chromatography
GPS Global positioning system
HASP Health and Safety Plan
HOPE High-density polyethylene
HMTD Hexamethylenetriperoxidediamine
hr Hour
i.d. Inner diameter
IATA International Air Transportation Association
IO Inorganic
ISO International Organization for Standardization
kg Kilogram
kPa Kilopascal
L Liter
Ibs Pounds
LRN Laboratory Response Network
Ltd. Limited
MARLAP Multi-Agency Radiological Laboratory Protocols
MCE Mixed cellulose ester
MDEA Methyldiethanolamine
mg Milligram
mL Milliliter
MPA Methylphosphonic acid
N Normal
NB Nitrobenzene
NEMI National Environmental Methods Index
IV
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NHSRC National Homeland Security Research Center
NIOSH National Institute for Occupational Safety and Health
NIST National Institute of Standards and Technology
n.o.s. Not otherwise specified (as used in 49 CFR Part 172)
NOS Not otherwise specified (as used in EPA's Standardized Analytical Methods for
Environmental Restoration Following Homeland Security Events)
NRC U.S. Nuclear Regulatory Commission
o.d. Outer diameter
ORISE Oak Ridge Institute for Science and Education
OSHA Occupational Safety and Health Administration
OSWER Office of Solid Waste and Emergency Response
OVS OSHA versatile sampler
OW Office of Water
PETN Pentaerythritol tetranitrate
PHMSA Pipeline and Hazardous Materials Safety Administration
ppb Parts per billion
PPE Personal protective equipment
ppm Parts per million
psi Pound-force per square inch
psig Pound-force per square inch gauge
PTFE Polytetrafluoroethylene (Teflon®)
PUF Polyurethene foam
PVC Polyvinylchloride
QC Quality control
qty Quantity
Rev. Revision
RDX Hexahydro-l,3,5-trinitro-l,3,5-triazine
SAM Standardized Analytical Methods for Environmental Restoration Following Homeland
Security Events
SCID Sample Collection Information Document
SCP Sample Collection Plan
SM Standard Method
spp. Species
SW Solid waste
TBD To be determined
TDG Thiodiglycol
TEA Triethanolamine
TI Transport Index
TNB Trinitrobenzene
TNT Trinitrotoluene
TO Toxic organic
UN United Nations
USGS United States Geological Survey
VCSB Voluntary Consensus Standards Body
VOA Volatile organic analyte
VOC Volatile organic compound
Vol. Volume
WEF Water Environment Federation
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Sample Collection Information Document
[Companion to Standardized Analytical Methods for Environmental
Restoration Following Homeland Security Events (SAM)]
1.0 Background
The U.S. Environmental Protection Agency's (EPA's) National Homeland Security Research
Center (NHSRC) has worked with experts from across EPA and its sister agencies since 2003 to
develop a compendium of analytical methods to be used when responding to national homeland
security related incidents. Analytical methods have been selected for chemical, radiochemical,
pathogen, and biotoxin analytes of concern for the types of environmental sample matrices that
are anticipated in such incidents. The results of these efforts have been published in several
revisions of EPA's Standardized Analytical Methods for Environmental Restoration Following
Homeland Security Events (SAM), available at http://www.epa.gov/sam. NHSRC periodically
reviews and updates the SAM document to address the needs of homeland security, reflect
improvements in analytical methods and new technologies, and incorporate changes in target
analytes.
During development of the SAM documents, EPA recognized the need for a companion
document to provide information regarding collection of samples for analysis by the methods
listed in SAM. This document is intended to address this need, in part, by providing information
regarding sample containers, preservation, size, packaging, and sources for additional
information supporting collection of samples to be analyzed using the methods listed in SAM
Revision 5.0. As with SAM, NHSRC plans to update the information in this document
periodically, to reflect changes to the analytes and/or methods listed in SAM.
The information contained in this document is intended to support and be used with the
methods listed in SAM Revision 5.0. The information will be reviewed and updated
periodically, along with the SAM document, to reflect advances in technologies, results of
method evaluation and validation studies, and additional analytes or matrices.
2.0 Scope and Application
This Sample Collection Information Document (SCID) provides general information for use by
EPA and its contractors when collecting samples during environmental remediation following a
homeland security event. The document is intended to be used with SAM, and to provide
information needed for collection of samples to be analyzed using the specific methods and
procedures listed in SAM Revision 5.O.* Where possible, the information provided was obtained
from the sample collection requirements and guidelines included in the SAM Revision 5.0
analytical methods. Where this information was not available, additional sources were used (see
Section 7.0).
The information in this document is intended to be used during site assessment, remediation, and
clearance activities following a homeland security event; it assumes that samples will be collected
by personnel trained in collection of environmental samples containing the target analytes and in
dealing with the corresponding safety concerns. Information is included regarding containers,
* SAM Revision 5.0 and its methods are available at: www.epa.gov/sam/pdfs/reportSAM092909.pdf
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collection volume or weight, preservation, holding times, and packaging of samples representing
the matrices listed in SAM, for measurement of the analytes listed in SAM, Revision 5.0.
Collection, preservation, and shipment procedures and information for samples containing
chemical warfare agents (CWAs) listed in SAM are currently being evaluated by EPA and,
therefore, are not included in this document at this time.
NOTE: It is possible that some of the information in this document should be modified to address
site- or event-specific data needs; for example, additional sample volume may be needed for quality
control (QC) or in cases when a low concentration of analyte is suspected. Sample Collection Plans
(SCPs) should be in place and consulted for specific sample collection requirements prior to initiation
of sample collection activities. Site- or event-specific SCPs include information regarding laboratory
capacity, the extent of contamination, target analytes, data quality objectives, sample locations, and the
number and type of samples needed.
2.1 Sample Collection Information Tables
This document contains the following four tables listing information for collection of
samples that will be analyzed for measurement of the analytes listed in SAM:
• Attachment A: Sample Collection Information for the Chemical Analytes Listed in
SAM
• Attachment B: Sample Collection Information for the Radiochemical Analytes
Listed in SAM
• Attachment C: Sample Collection Information for the Pathogens Listed in SAM
• Attachment D: Sample Collection Information for the Biotoxin Analytes Listed in
SAM
Each table provides the sample size that should be collected to support sample analysis,
the preservatives and/or temperature needed to maintain sample integrity prior to
analysis, the maximum amount of time that should elapse between sample collection and
the initiation of analytical procedures (e.g., sample analysis, digestion, inoculation), the
appropriate type of container, the sample label and packaging procedures needed for
sample shipment, and the source(s) used to provide the information.
2.2 Document Development
EPA developed a hierarchy of references to prioritize the documents and resources that
were used to identify the information that is included in this document. The first sources
consulted were the methods listed in SAM Revision 5.0. If those methods included
sample collection information, the information was evaluated and, if appropriate,
included in the sample collection information tables. The second sources consulted were
EPA procedures for collection of samples addressing the specific analyte/matrix pair. If
there were no EPA procedures available, other federal agency or Voluntary Consensus
Standards Body (VCSB) methods were consulted. If no procedures were identified for
collection of a particular analyte/matrix combination, methods for that analyte in other
matrices were considered, followed by procedures described and supported by data in
research literature, such as journal articles. The following agencies, organizations, and
publications were used:
• U.S. EPA - United States Environmental Protection Agency
• AOAC - AOAC International (formerly Association of Official Analytical Chemists)
• ASTM - ASTM International (formerly American Society for Testing and Materials)
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• CDC - Centers for Disease Control and Prevention
• CFR - Code of Federal Regulations
• U.S. Army
• U.S. DHS - United States Department of Homeland Security
• U.S. DOL - United States Department of Labor
• U.S. DOT - United States Department of Transportation
• U.S. DOE - United States Department of Energy
• U.S. FDA - United States Food and Drug Administration
• USGS - United States Geological Survey
• IATA - International Air Transport Association
• ISO - International Organization for Standardization
• LRN - Laboratory Response Network
• Minnesota Department of Agriculture
• NEMI - National Environmental Methods Index
• NIOSH - National Institute for Occupational Safety and Health
• Organisation for the Prohibition of Chemical Weapons
• OSHA - Occupational Safety and Health Administration
• Standard Methods for the Examination of Water and Wastewater
• Washington State Department of Ecology
• Journals: Analyst, Analytical Letters, Applied and Environmental Microbiology,
Atmospheric Environment, Biomedical Chromatography, Chromatographia, Current
Protocols in Microbiology, FEMS Microbiology Letters, Journal of Chromatography
A, Journal of Chromatography B, Journal of Forensic Sciences, Journal of
Virological Methods, Public Health Reports
Sample collection information was not identified for all the analyte/matrix pairs listed in
SAM. In some cases, "TBD" (to be determined) is used to identify gaps of information
that have yet to be determined. In other cases, a footnote is used to identify information
that is considered to be applicable to a given analyte/matrix combination because the
information is specific to a similar analyte or matrix.
2.3 Limitations
This document provides summary information only regarding collection of samples to be
analyzed for measurement of the target analytes listed in SAM. Although at this time
much of the information has not been tested for a particular analyte (e.g., analytes not
explicitly identified in the method) or matrix, the information listed is considered to be
the most appropriate currently available information. For example, research is needed to
determine appropriate preservation and holding times for many of the chemical and
biological agents. Many of the target analytes listed in this document have only recently
become an environmental concern, and EPA is actively pursuing development and
validation of appropriate sample collection procedures.
Sample Collection Plans must be consulted for site- or event-specific requirements,
including QC and reporting. The information sources cited in this document also should
be consulted for additional details regarding sample collection, including QC
requirements, sample handling, packaging, shipping, and safety procedures. Samplers
should check with the incident commanders for special instructions regarding evidentiary
matters prior to sample collection.
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3.0 Health and Safety Considerations
This document assumes that a site- or event-specific Health and Safety Plan (HASP) is in place
that includes the safety concerns and requirements regarding the specific types of hazards that
should be considered during a sample collection event. This section provides general guidelines
regarding health and safety concerns when sampling environmental material in response to a
homeland security event. At a minimum, all sampling team members should be trained in
Occupational Safety and Health Administration (OSHA) requirements for hazardous waste
operations and emergency response at 29 CFR 1910.120 or 29 CFR 1926.65 and have current
medical screening.
3.1 Health and Safety Plans
Health and Safety Plans (HASPs) will vary depending on the site, the sampling phase
(site assessment, remediation, or final status determination), and the responsible
organization. The purpose of these plans is to ensure maximum protection to workers,
the environment, and surrounding communities, in a way that is consistent with
requirements needed to perform operational activities. At a minimum, HASPs should
include instructions and guidelines regarding:
• Names, positions, and contact information of key personnel and health and safety
personnel
Site- or event-specific risk assessment addressing sample collection activities
• Training requirements
Personal protective equipment (PPE) on site and usage requirements
• Medical screening requirements (maintain confidential documents properly and
securely)
Site or event control
• Emergency response plan, containing off-site emergency contact information such as
local hazardous materials response teams or additional trained rescue personnel (29
CFR 1910.38)
• Entry procedures
Spill containment
Decontamination procedures
NOTE: Entry and decontamination procedures should address personnel monitoring and
decontamination during entry and egress.
3.2 Personal Protective Equipment
Each site or event also will dictate the level of PPE that will be required. Selection of
protective clothing is dependent on site conditions and sample collection requirements
included in the SCP. Specific guidance for selection of PPE is provided in 29 CFR
1910.120, Appendix B. Factors that should be considered during selection include:
contaminant identification, routes of exposure (i.e., inhalation, skin absorption, ingestion,
and injection), performance of equipment in protecting against exposure, activity
duration, and stress induced by work requirements. Because the use of PPE can also
cause hazards to workers (e.g., heat stress, impaired vision and mobility), care should be
taken to provide a level of protection that is sufficient to prevent exposure yet is not too
high so as to create other unnecessary hazards.
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3.3 Training
Sample collectors must be trained in collection and handling of samples suspected of
containing the contaminants of concern (see Attachments A through D), must be up to
date regarding medical screening requirements, and must be approved for site entry.
Additionally, sample collectors must be trained in the following:
Ability to select and work with the appropriate level of PPE
Decontamination procedures
• Prevention of sample cross-contamination
4.0 Preparation for Sample Collection
It is highly recommended that sampling kits be used during sample collection, and that these kits
be properly equipped, maintained, and organized before deployment of sample collection
personnel. Sample collectors should consult with project managers and the SCP to determine
what equipment and materials should be assembled. Sample kits should contain all sample
containers, materials, supplies, and forms needed to perform sample collection, decontamination,
documentation, and field packaging activities.
4.1 Field Sampling Equipment and Supplies
Before starting field sampling activities, all necessary equipment and supplies should be
identified and available. The following is a preliminary list of equipment that needs to be
specified and available (from U.S. Geological Survey [USGS], National Institute of
Standards and Technology [NIST], U.S. Nuclear Regulatory Commission [NRC], and
Multi-Agency Radiological Laboratory Protocols [MARLAP]):
Sampling devices (e.g., air filters, soil samplers, water samplers, air filter samplers)
• Sample preservation equipment (e.g., acids, dechlorinating reagents)
Sample volumetric measuring devices and/or weighing devices
Sample containers and packaging equipment
• PPE
Record keeping devices (e.g., logs, Chain-of-Custody [COC] forms, writing
instruments)
• Site maps, global positioning system (GPS) recorders, etc.
Sample location markers
• Pre-labeled and pre-weighed sampling containers
• Shipping containers, shipping forms, and shipping labels
4.2 Field Data Documentation
All data collected in the field should be adequately documented. Documented
information should include (for example):
Names of field sampling personnel
• Sample Collection Plan (SCP)
Sample location(s)
Sampling depth
• Physical and meteorological conditions
Date and time of sampling
Sample medium
• Expected radionuclides (if applicable)
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Sample identification number
Sample size (weight, volume), sample duration (air filters), air volume, etc.
Sample handling precautions
5.0 Preparation of Sample Containers
5.1 Sample Container Labels
Each sample container has a label that provides information uniquely identifying and
describing the sample. A single, unique label is affixed to each individual sample
container, sample information added in waterproof ink, and the label is covered with
clear tape. Alternatively, a pre-prepared bar code that tracks the sample information can
be affixed to the container. Sample container labels or bar codes include information
regarding the date and time of sample collection; date, time and type of sample
preservation; sample identification numbers; and the names and signatures of sample
collectors.
5.2 Preparing Sample Containers for Packaging
Once samples have been collected and preserved as specified, the containers are prepared
for shipment to the analytical laboratory. Sample containers should be labeled, sealed,
and cleaned or disinfected prior to packing into transport or shipping containers.
Summary procedures for cleaning and sealing sample containers are provided in the
tables included in Attachments A through D. General information regarding packing
samples into transport containers outside the contaminated area is provided as a footnote
to each table.
5.3 General Sample Shipment Guidelines
Samplers are responsible for ensuring compliance with DOT and the IATA regulations
regarding the transfer of hazardous substances and environmental samples. These
regulations, 49 CFR 172 and 173 or DOT (organized by separate subparts as references
in Section 7.0) and the Dangerous Goods Regulations (DGR) for IATA, provide specific
details regarding proper marking, labeling, placarding, packaging, and shipment of
hazardous materials, substances and wastes, and regulatory exceptions and must be
consulted prior to preparation of or planning for sample shipment. Summary information
regarding the appropriate labeling and packaging of sample transport containers is
provided in the sample collection information tables included in Attachments A through
D.
5.4 Chain-of-Custody Forms
Chain-of-Custody (COC) forms create a written record that can be used to trace the
creation, possession, and handling of the sample from the moment of its collection
through analysis. A COC form accompanies each sample or group of samples as custody
of the sample(s) is transferred from one custodian to another. One copy of the form is
retained by the original sample collector. If multiple laboratories are receiving a sample,
individual COCs are provided to each individual laboratory, each COC representing the
contents of the sample shipment. Sample collectors are responsible for the initial
maintenance and completion of COC forms. Although COC forms vary in style, format,
and detail, the forms should contain the same minimal information required to identify
the sample. At a minimum, sample collectors are responsible for providing the following
information on the COC form:
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• General incident information (sample owners, contact information, site name)
Sample information (e.g., sample identification number, sample type [matrix],
whether grab or composite, number and type of sample containers, and date/time
sample was collected)
Date and time the sample was relinquished
Signature of persons transferring and receiving the samples
5.5 Custody Seals / Tamper-evident Bags
Custody seals are attached over the cap of each sample container to ensure the sample has
not been opened or tampered with after collection and packaging. A custody seal also
can be placed over the shipping or transport container, making it impossible to open the
container without ripping the seal. Typically, there is one seal per sample container and
two seals placed on opposite sides of the shipping container. Custody seals contain the
signature of the person responsible for packing the container and the date sealed. The
seal must be sturdy to resist incidental contact but able to break when the cap or lid is
moved. Sample collectors should:
Sign and date the sample custody seal, usually a 1- by 3-inch white paper label with
black lettering and an adhesive backing. The custody seal is part of the COC process
and is used to prevent or identify tampering with samples.
Place the custody seal across the container lid so that the seal would be broken if a
container were to be opened. This often requires multiple seals covering any
opening. If a cooler is used, ensure that the water drainage point is secure.
Alternatively, sample containers may be placed into a tamper-evident bag. Bags/seals
with a unique identifier must be associated with the person collecting the sample through
logbook entries, preservation of tear-off strips in the logbook, or similar means. When
using a tape-type tamper-evident seal, the initials of the person securing the container and
the date that it was secured must be recorded on the seal.
6.0 Definitions
The following definitions are provided to describe the information listed in the sample collection
tables:
Analyte - The compound or class of compounds that will be measured in the sample
collected. The analytes in this document are identical to those listed in SAM Revision 5.0.
Container - The type of container (e.g., bottle, bag) that must be used to hold the sample.
The container must be sufficient to maintain sample integrity and be composed of materials
that will remain inert when in contact with the sample.
• Holding Time - The maximum amount of time allowable from sample collection until
sample analysis, extraction, or inoculation.
• Matrix - The principal material of which the sample is composed. The matrices in this
document are identical to the matrices listed for each analyte in SAM Revision 5.0.
Packaging - Sample container packaging requirements for shipment of sample to the
laboratory.
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Preservation - Conditions and/or chemicals used to maintain the integrity of a sample.
Some common preservatives include nitric acid, hydrochloric acid, sodium thiosulfate, and
temperatures < 4°C (chemical analytes) or < 10°C and above freezing (biological analytes).
Sample Size - The minimum amount of sample that should be collected to support analysis
of a single sample. Volume and weight requirements depend on the target analyte(s), the
analytical method that will be used, and the data requirements.
Shipping Label - U.S. DOT shipping label requirements under 49 CFR 172 and 173.
Source - The reference(s) supporting the information that is provided in the table.
7.0 References
Analytical methods listed in Attachments A through D can be accessed through SAM at
http://www.epa.gov/sam/. In addition to these methods, the following resources were used to
prepare this document:
AOAC International. Method 993.06: "Staphylococcal Enterotoxins in Selected Foods."
Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, Volume I.
• Centers for Disease Control and Prevention. "Emergency Preparedness and Response,
Bioterrorism Agents and Diseases." http://www.bt.cdc.gov/agent/agentlist-category.asp
(accessed October 7, 2009).
• Duchaine, C., Thorne, P.S., Meriaux, A., Grimard, Y., Whitten, P., and Cormier, Y. 2001.
"Comparison of Endotoxin Exposure Assessment by Bioaerosol Impinger and Filter-
Sampling Methods" Applied and Environmental Microbiology. 67(6): 2775-2780.
• Fout, G.S., Martinson, B.C., Moyer, M.W.N., and Dahling, D.R. June 2003. "A Multiplex
Reverse Transcription-PCR Method for Detection of Human Enteric Viruses in
Groundwater." Applied and Environmental Microbiology. 69(6): 3158-3164.
• Hiemstra, M., de Kok, A. 2007. "Comprehensive Multi-residue Method for the Target
Analysis of Pesticides in Crops Using Liquid Chromatography-tandem Mass Spectrometry."
Journal of Chromatography A. 1154(1): 3-25.
Huq, A., Grim, C., Colwell, R., and Nair, G.B. September 2006. "Detection, Isolation, and
Identification of Vibrio cholerae from the Environment." Current Protocols in Microbiology.
6A.5.1-6A.5.3.8.
Hunt, M.E. and Rice, E.W. 2005. Part 9000, "Microbiological Examination." E.F. Askew and
R-K Smith. Part 4000, "Inorganic Nonmetallic Constituents." In Eaton, A.D., Clesceri, L.S.,
Rice, E.W., Greenberg, A.E., and M.A.H. Franson (eds.). Standard Methods for the
Examination of Water and Wastewater, 21st Edition. American Public Health Association,
American Water Works Association, and Water Environment Federation.
• International Air Transport Association. 2009. "Guidance Document - Infectious
Substances." International Civil Aviation Organization Dangerous Goods Panel.
http://www.iata.org/NR/rdonlvres/9C7E382B-2536-47CE-84B4-
9A883ECFA040/0/GuidanceDoc62DGR 50.pdf (accessed October 6, 2009).
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James, K.J., and Sherlock, I.R., 1996. Determination of the Cyanobacterial Neurotoxin,
Anatoxin-A, by Derivatization Using 7-Fluoro-4-Nitro-2,l,3-Benzoxadiazole (NBF-F) and
HPLC Analysis with Fluorimetric Detection. Biomedical Chromatography. 10: 46 - 47.
Johnson, B.J., Emerson, D.W., Song, L., Floyd, J., and Tadepalli, B. 1999. "Determination of
Active Chlorine in Air by Bonded Phase Sorbent Collection and Spectophotometric
Analysis." Analyst. 124(12): 1853-1857.
Jothikumar, N., Kang, G., and Hill, V.R. 2009. "Broadly Reactive TaqMan® Assay for Real-
Time RT-PCR Detection of Rotavirus in Clinical and Environmental Samples." Journal of
Virological Methods. 155: 126-131.
Kruszyna, R., Smith, R.P., and Kruszyna, H. 1998. "Determining Sodium Azide
Concentration in the Blood by Ion Chromatography." Journal of Forensic Sciences. 43(1):
200-202.
Lawton, L.A., Edwards, C., and Codd, G.A. 1994. "Extraction and High-Performance Liquid
Chromatographic Method for the Determination of Microcystins in Raw and Untreated
Waters." Analyst. 119(7): 1525-1530.
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-------
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idx?c=ecfr&sid=4a9f058a58f4bfdc9f73305868931710&rgn=div8&view=text&node=49:2.1.
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(poisonous materials)." http://ecfr.gpoaccess.gov/cgi/t/text/text-
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Jdx?c=ecfr;sid=6825997bfe550b32bd2e47d3 lcbe7802:rgn=div5;view=text:node=49%3A2.1.
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Packaging" and "Packages and Additional General Requirements for Non-bulk Packaging
and Packages." http://ecfr.gpoaccess.gov/cgi/t/text/text-
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Jdx?c=ecfr:sid=6825997bfe550b32bd2e47d3 lcbe7802:rgn=div5:view=text:node=49%3A2.1.
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October 9, 2009).
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Jdx?c=ecfr:sid=6825997bfe550b32bd2e47d3 lcbe7802:rgn=div5:view=text:node=49%3A2.1.
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idx?c=ecfr&sid=5799d0619c2f5904440fa51d93e208c6&rgn=div9&view=text&node=40:8.0.
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(accessed October 6, 2009).
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"Simultaneous Determination of two Accute Poisoning Rodenticides Tetramine and
Fluoroacetamide with a Coupled Column in Poisoning Cases." Journal of Chromatography
B. 876(1): 103-108.
12 June 2010
-------
Attachment A
Sample Collection Information for the Chemical Analytes
Listed in SAM
Sample Collection Information Document June 2010
-------
Attachment A: Sample Collection Information for the Chemical Analytes Listed in SAM
Air Samples
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)
Aldicarb sulfone
Aldicarb sulfoxide
Allyl alcohol'51
4-Aminopvridine
Sample
Volume'11
TBD
120L(at1
L/min)
120L(at1
L/min)
240 - 480 L
240 - 480 L
240 - 480 L
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
Sample Container
250-mL PTFE centrifuge tube
Filter / solid sorbent tube (OVS-7
tube: 13mm quartz fiber filter,
XAD-7[270mg/140mg])
Filter / solid sorbent tube (OVS-7
tube: 13mm quartz fiber filter,
XAD-7[270mg/140mg])
Filter / solid sorbent tube (OVS-2
tube: 13mm quartz fiber filter,
XAD-2[270mg/140mg])
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Holding Time
TBD
Stable at least 4
days at
temperatures up
to 45°C.
Stable at least 4
days at
temperatures up
to 45°C.
Stable at least 30
days at negative
(-) 12°Corat
least 7 days at
24°C.
TBD
TBD
Under conditions
of normal use,
most VOCs can
be recovered
from canisters
near their original
concentrations
after storage
times of up to 30
days.
Sample
Preservation
or Preparation
TBD
None
None
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
None
Packaging Requirements
TBD
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Ship canister in container provided by laboratory.
Shipping Label'2'
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Acrylamide, solid, 6.1,
Poison, UN2074
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid, 6.1,
Poison, UN1093
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Allyl alcohol, 3,
Flammable liquid, 6.1,
Poison, UN1098.
Source/SAM
Method'3'
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
PV2004
(OSHA)
PV2004
(OSHA)
5601
(NIOSH)
5601
(NIOSH)
5601
(NIOSH)
TO- 15
(EPAORD)
Not of concern in this sample type
Sample Collection Information Document
A1 -1
June 2010
-------
Air Samples
Analyte
Ammonia
Ammonium metavanadate
(analyze as total vanadium)
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Arsine
Asbestos
Boron trifluoride
Brodifacoum
Bromadiolone
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)
Carfentanil
Sample
Volume'11
0.1 (at 50 ppm) -
96 L
Up to 1700m3
Up to 1700m3
Up to 1700m3
0.1 L (at 0.05
ppm) - 1 0 L
500 - 5000 L
Up to 480 L
Sample Container
Solid sorbent tube (sulfuric acid-
treated silica gel)
Filter (quartz, silica, or cellulose
fiber)
Filter (quartz, silica, or cellulose
fiber)
Filter (quartz, silica, or cellulose
fiber)
Solid sorbent tube (coconut shell
charcoal [100 mg/50 mg])
Polycarbonate or cellulose ester
filter
Midget glass bubbler containing
10-mL of 0.1 M NH4F; sample
solution transferred to 20-mL
glass vial with PTFE cap
Holding Time
1 day after
desorption.
6 months
6 months
6 months
6 days
None
TBD
Sample
Preservation
or Preparation
After desorption,
adjust pH of the
desorption solution
to between 5 - 6.5
with NaOH.
None
None
None
25°C
None
TBD
Packaging Requirements
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps immediately after sampling. Place
tubes in double plastic bags and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps immediately after sampling. Place
tubes in double plastic bags and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND (if
greater than 10%
ammonia by volume)
Ammonia solution, 8,
Corrosive, UN2672
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Aresnic trioxide, 6.1 ,
Poison, UN1561
Standard carrier
shipping label AND
Arsine, 2.3 (2.1),
Poisonous gas,
UN2190
Standard carrier
shipping label AND
Asbestos, 9, Class 9,
NA2212
Standard carrier
shipping label AND
Boron trifluoride, 2.3,
Poisonous gas,
UN1008
Source/SAM
Method'31
6015
(NIOSH)
10-3.1 /IO-3.4/IO
3.5
(EPAORD)
10-3.1 /IO-3.4/IO
3.5
(EPAORD)
IO-3.1 /IO-3.4/IO
3.5
(EPAORD)
6001
(NIOSH)
10312:1995
(ISO)
ID216SG
(OSHA)
Not of concern in this sample type
Not of concern in this sample type
Up to 1700m3
240 - 480 L
Filter (quartz, silica, or cellulose
fiber)
Filter / solid sorbent tube (OVS-2
tube: 13mm quartz fiber filter,
XAD-2[270mg/140mg])
6 months
Stable at least 30
days at negative
(-) 12°Corat
least 7 days at
24°C.
None
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
10-3.1 /IO-3.4/IO
3.5
(EPAORD)
5601
(NIOSH)
Not of concern in this sample type
Sample Collection Information Document
A1 -2
June 2010
-------
Air Samples
Analyte
Carbon disulfide
Chlorfenvinphos
Chlorine'51
2-Chloroethanol
3-Chloro-1,2-propanediol'5'
Chloropicrin'5'
Chlorpyrifos
Chlorpyrifos oxon
Crimidine
Sample
Volume'11
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
240 - 7200 L
(at 1 - 5 L/min)
8 L (at 0.1 ppm)
-360 L
2 (at 5 ppm) - 35
L (at 0.01 -0.2
L/min)
240 - 7200 L
(at 1 - 5 L/min)
5 L (at 0.2 L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sample Container
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Prefilter and filter (PTFE, 0.5 |jm
and silver membrane, 25 mm,
0.45 |jm)
Solid sorbent tube (petroleum
charcoal [100 mg/50 mg])
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Solid sorbent tube (XAD-4 [100
mg/50 mg])
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Holding Time
Under conditions
of normal use,
most VOCs can
be recovered
from canisters
near their original
concentrations
after storage
times of up to 30
days.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Up to 30 days at
25°C.
TBD
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample
Preservation
or Preparation
None
Store at < 4°C.
Protect from light.
None
Store at < 4°C.
Room temperature
in the dark.
Store at < 4°C.
Store at < 4°C.
Packaging Requirements
Ship canister in container provided by laboratory.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid,
UN1131
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Chlorine, 2.3, 5.1,
Oxidizer, 8, Poison
gas, UN1017
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'3'
TO- 15
(EPAORD)
TO-10A
(EPAORD)
Adapted from
Analyst, 124(12):
1853-1857/4500-
Cl G (SM)
2513(NIOSH)
TO-10A
(EPAORD)
PV2103
(OSHA)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
Sample Collection Information Document
A1 -3
June 2010
-------
Air Samples
Analyte
Cyanide, Amenable to
chlorination
Cyanide, Total'51
Cyanogen chloride'5'
1,2-Dichloroethane
(degradation product of HD)
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl
methylphosphonate (DIMP)
(degradation product of
GB)<5>
Sample
Volume'11
Sample Container
Holding Time
Sample
Preservation
or Preparation
Packaging Requirements
Shipping Label'2'
Source/SAM
Method'3'
Not of concern in this sample type
2 (at 5 ppm) - 90
L
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Solid sorbent tube (soda lime
[600 mg/200 mg])
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Stable at least 2
weeks at 25°C.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Under conditions
of normal use,
most VOCs can
be recovered
from canisters
near their original
concentrations
after storage
times of up to 30
days.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Store at 25°C.
None
None
Store at < 4°C.
Store at < 4°C.
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Ship canister in container provided by laboratory.
Ship canister in container provided by laboratory.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label AND
Cyanides, inorganic,
solid, n.o.s, 6.1,
Poison, UN1588
Standard carrier
shipping label AND
Cyanogen chloride,
2.3, Poisonous gas,
UN1589
Standard carrier
shipping label AND
Ethylene dichloride, 6,
Poison, UN1184
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
6010
(NIOSH)
TO- 15
(EPAORD)
TO- 15
(EPAORD)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
240 - 7200 L
(at 1 - 5 L/min)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Store at < 4°C.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label
TO-10A
(EPAORD)
Sample Collection Information Document
A1 -4
June 2010
-------
Air Samples
Analyte
Dimethylphosphite151
Dimethylphosphoramidic
acid (degradation product of
GA)<51
Diphacinone
Disulfoton
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of HD)
EA2192
[Diisopropylaminoethyl
methyl thiolophosphonate]
(hydrolysis product of VX)|S|
Ethyl methylphosphonic
acid (EMPA)
(degradation product of
VX)'5'
Sample
Volume'11
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sample Container
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample
Preservation
or Preparation
Store at < 4°C.
Store at < 4°C.
Packaging Requirements
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Source/SAM
Method'31
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
12- 240 L
12- 240 L
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
10daysat25°C
or
30 days at 0°C.
10daysat25°C
or
30 days at 0°C.
10 days at 25°C or
30 days at 0°C.
10daysat25°Cor
30 days at 0°C.
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
5600
(NIOSH)
5600
(NIOSH)
Not of concern in this sample type
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Store at < 4°C.
Store at < 4°C.
TBD(7)
Air-tight container containing activated carbon
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
TBD(7)
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
TO-10A
(EPAORD)
TO-10A
(EPAORD)/
Footnote (8)
Sample Collection Information Document
A1 -5
June 2010
-------
Air Samples
Analyte
Ethyldichloroarsine (ED)'5'
N-Ethyldiethanolamine
(EDEA) (degradation
product of HN-1)'5'
Ethylene oxide
Fenamiphos'5'
Fentanyl
Fluoride
Fluoroacetamide
Fluoroacetic acid and
fluoroacetate salts (analyze
for fluoroacetate ion)'5'
2-Fluoroethanol
Sample
Volume'11
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
240 - 7200 L
(at 1 - 5 L/min)
One to four 1-L
or 6-L samples
(depending on
suspected
concentration),
each in a
separate
canister'6'
240 - 7200 L
(at 1 - 5 L/min)
Sample Container
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Specially prepared 1-L or 6-L
stainless steel canister
For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the sample
is collected using a pump and
flow control arrangement to
achieve a typical 101 - 202 kPa
(15-30psig)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Under conditions
of normal use,
most VOCs can
be recovered
from canisters
near their original
concentrations
after storage
times of up to 30
days.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample
Preservation
or Preparation
None
Store at < 4°C.
None
Store at < 4°C.
Packaging Requirements
Ship canister in container provided by laboratory.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Ship canister in container provided by laboratory.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Ethyldichloroarsine,
6.1, Poison, UN1892
Standard carrier
shipping label
Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to 40
CFR 172.101 for
more information)
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
TO- 15
(EPAORD)
TO-10A
(EPAORD)
TO- 15
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
Not of concern in this sample type
TBD
60 |JL (300.1)-
480 L (at 1 .5 -
2.0 L/min) (S301-
1)
2 (at 5 ppm) - 35
L (at 0.01 -0.2
L/min)
TBD
Cellulose ester membrane filter /
cleaned and rinsed glass or
plastic bottles
Solid sorbent tube (petroleum
charcoal [100 mg/50 mg])
TBD
28 days
TBD
TBD
None
None
TBD
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Fluoroacetic acid, 6.1 ,
Poison, UN2642
Standard carrier
shipping label
Adapted from
Journal of
Chromatography
6,876(1): 103-
108
S301-1 (NIOSH)/
300.1, Rev 1.0
(EPA OW)
2513
(NIOSH)
Sample Collection Information Document
A1 -6
June 2010
-------
Air Samples
Analyte
Formaldehyde
Gasoline Ranqe Orqanics
Hexahydro-1 ,3,5-trinitro-
1,3,5-triazine(RDX)
Hexamethylenetriperoxide-
diamine (HMTD)
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide
Hydrogen fluoride
Hydrogen sulfide
Isopropyl methylphosphonic
acid (IMPA) (degradation
product of GB)'S|
Kerosene
Lead arsenate
(analyze for total arsenic)
Sample
Volume'11
1 L (at 0.25
mg/m3) - 1 5 L (at
2.5 mg/m3)
Sample Container
Dinitrophenylhydrazine (DNPH)-
coated silica gel cartridge
Holding Time
At least 34 days
at 5°C.
Sample
Preservation
or Preparation
Store at 5°C.
Packaging Requirements
Place end caps onto the cartridge and seal
sampler in an envelope. Protect from heat. Place
cartridge in double plastic bags and wrap with
bubble wrap. Ship samples on ice. Pack samples
as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to 40
CFR 172.101 for
more information).
Source/SAM
Method'31
2016
(NIOSH)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
3-100L
3-100L
2 - 90 L (at 5
ppm)
3-100L
1 .2 (at 1 0 ppm) -
40 L
240 - 7200 L
(at 1 - 5 L/min)
Solid sorbent tube (washed silica
gel [400 mg/200 mg] with glass
fiber filter plug)
Solid sorbent tube (washed silica
gel [400 mg/200 mg] with glass
fiber filter plug)
Solid sorbent tube (soda lime
[600 mg/200 mg])
Solid sorbent tube (washed silica
gel [400 mg/200 mg] with glass
fiber filter plug)
Filter and solid sorbent tube
(Zefluor, 0.5 |jm; coconut shell
charcoal [400 mg/200 mg])
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
At least 21 days
at 25°C.
At least 21 days
at 25°C.
At least 21 days
at 25°C.
At least 21 days
at 25°C.
At least 21 days
at 25°C.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Store at 25°C.
Store at 25°C.
Store at 25°C.
Store at 25°C.
Store at 25°C.
Store at < 4°C.
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Place filter or cleaned sorbent tube in double
plastic bags, and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label AND
Hydrogen bromide,
2.3, Poisonous gas,
UN1048
Standard carrier
shipping label AND
Hydrogen chloride,
2.3, Poisonous gas,
UN1050
Standard carrier
shipping label AND
Hydrogen cyanide,
2.3, Poisonous gas,
UN1956
Standard carrier
shipping label AND
Hydrogen fluoride,
2.3, Poisonous gas,
Corrosive, UN1052
Standard carrier
shipping label AND
Hydrogen sulfide, 2.3,
Poisonous gas,
Corrosive, UN1053
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
7903
(NIOSH)
7903
(NIOSH)
6010
(NIOSH)
7903
(NIOSH)
6013
(NIOSH)
TO-10A
(EPAORD)
Not of concern in this sample type
Up to 1700m3
Filter (quartz, silica, or cellulose
fiber)
6 months
None
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
IO-3.1 /IO-3.4/IO
3.5
(EPAORD)
Sample Collection Information Document
A1 -7
June 2010
-------
Air Samples
Analyte
Lewisite oxide
(degradation product of
Lewisite)'51
Mercuric chloride (analyze
for total mercury)
Mercury, Total
Methamidophos
Methomyl
Methoxyethylmercuric
acetate (analyze for total
mercury)'5'
Methyl acrylonitrile
Methyl fluoroacetate
(analyze for fluoroacetate
ion)'51
Sample
Volume'11
Up to 1700m3
Sample Container
Filter (quartz, silica, or cellulose
fiber)
Holding Time
6 months
Sample
Preservation
or Preparation
None
Packaging Requirements
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Arsenic compounds,
solid, n.o.s, 6.1,
Poison, UN1557
Source/SAM
Method'3'
10-3.1 /IO-3.4
/ IO-3.5
(EPAORD)/
Footnote (8)
Not of concern in this sample type
For vapor phase
Hg, the volume
of air sampled is
determined from
the average of
the flow rate
measurements
and the sample
duration.
Typical flow rate
is 30 L/minfor 12
-24 hours.
TBD
12- 480 L
For vapor phase
Hg, the volume
of air sampled is
determined from
the average of
the flow rate
measurements
and the sample
duration.
Typical flow rate
of 30 L/minfor 12
-24 hours.
120 L (at 1
L/min)
Up to 480 L (at
1.5 -2.0 L/min)
Sampling pumps specially
designed for trace-level pollutant
sampling; vapor phase Hg
collected using two gold traps in
series; the two traps are attached
to a PTFE filter pack.
250-mL PTFE centrifuge tube
Filter / solid sorbent tube (OVS-2
tube: 13mm quartz fiber filter,
XAD-2[270mg/140mg])
Sampling pumps specially
designed for trace-level pollutant
sampling; vapor phase Hg
collected using two gold traps in
series; the two traps are attached
to a PTFE filter pack.
Filter / solid sorbent tube (OVS-7
tube: 13mm quartz fiber filter,
XAD-7[270mg/140mg])
Cellulose ester membrane filter /
cleaned and rinsed glass or
plastic bottles
Vapor phase Hg
samples can be
stored up to 1
week at 4°C.
Particle-phase
Hg filters can be
stored indefinitely
in a freezer at
negative (-)
40°C.
Analyze
immediately or
store at negative
(-) 18°C.
Stable 30 days at
negative (-) 12°C
or at least 7 days
at 24°C.
Vapor phase Hg
samples can be
stored up to 1
week at 4°C.
Particle-phase
Hg filters can be
stored indefinitely
in a freezer at -
negative (-)
40°C.
Stable at least 4
days at
temperatures up
to 45°C.
28 days
Store vapor phase
Hg samples at 4°C.
Store particle-
phase Hg filters
indefinitely at
negative (-) 40°C.
TBD
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Store vapor phase
Hg samples at 4°C.
Store particle-
phase Hg filters
indefinitely at
negative (-) 40°C.
None
None
Wipe the outside of each trap clean, cap both
ends. Place trap in double plastic bags and wrap
with bubble wrap. Pack samples as described in
Footnote (4).
TBD
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Wipe the outside of each trap clean, cap both
ends. Place trap in double plastic bags and wrap
with bubble wrap. Pack samples as described in
Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Standard carrier
shipping label AND
Mercury, 8,
Corrosive, UN2809
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Mercury compounds,
solid, n.o.s, 6.1,
Poison, UN2025
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
IO-5
(EPAORD)
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
5601
(NIOSH)
IO-5
(EPAORD)
PV2004
(OSHA)
S301-1 (NIOSH)/
300.1, Rev 1.0
(EPA OW)
Sample Collection Information Document
A1 -8
June 2010
-------
Air Samples
Analyte
Methyl hydrazine
Methyl isocyanate
Methyl paraoxon
Methyl parathion
Methylamine
N-Methyldiethanolamine
(MDEA) (degradation
product of HN-2)'51
Methylphosphonic acid
(MPA)
(degradation product of VX,
GB, & GD)'51
Mevinphos
Monocrotophos
Sample
Volume'11
3 (at 0.2 ppm) -
20 L
15 L (at 0.05
L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
10 L (at 0.2
L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sample Container
Bubbler (0.1 M hydrochloric acid)
Solid sorbent tube (XAD-7 tubes
coated with 0.03 mg of 1-(2-
pyridyl)piperazine [1-2PP])
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Solid sorbent tubes containing
XAD-7 resin coated with 10%
NBD chloride by weight
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Holding Time
At least 5 days at
25°C.
Can be stored at
room
temperature or
refrigerated for
up to 18 days.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
TBD
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample
Preservation
or Preparation
Store at 25°C.
Store at reduced
temperature out of
sunlight until
analysis.
Store at < 4°C.
Store at < 4°C.
TBD
Store at < 4°C.
Store at < 4°C.
Store at < 4°C.
Store at < 4°C.
Packaging Requirements
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap each glass container
with bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Cap ends of tube and wrap
with OSHA seal. Seal the container with non-
reactive tape or film. Wrap each glass container
with bubble wrap. Pack samples as described in
Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Cap ends of tube and wrap
with OSHA seal. Seal the container with non-
reactive tape or film. Wrap each glass container
with bubble wrap. Pack samples as described in
Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Methyl hydrazine, 6.1,
Poison, UN1244
Standard carrier
shipping label AND
Methyl hydrazine, 6.1,
Poison, UN2480
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND,
Methylamine,
anhydrous, 2.1,
Flammable gas,
UN1061
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
3510
(NIOSH)
OS HA 54
TO-10A
(EPAORD)
TO-10A
(EPAORD)
OS HA 40
TO-10A
(EPAORD)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Sample Collection Information Document
A1 -9
June 2010
-------
Air Samples
Analyte
Nicotine compounds
(analyze as nicotine)
Octahydro-1 ,3,5,7-tetranitro-
1,3,5,7-tetrazocine (HMX)
Organophosphate
pesticides, NOS
Osmium tetraoxide
(analyze for total osmium)'5'
Oxamyl
Paraquat
Paraoxon
Parathion
Pentaerythritol tetranitrate
(PETN)
Phencyclidine151
Phenol'51
Phorate
Sample
Volume'11
Sample Container
Holding Time
Sample
Preservation
or Preparation
Packaging Requirements
Shipping Label'2'
Source/SAM
Method'3'
Not of concern in this sample type
Not of concern in this sample type
12- 240 L
TBD
240 - 480 L
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
High volume filter material (may
be associated with either the TSP
or PM10 sampler)
Filter / solid sorbent tube (OVS-2
tube: 13mm quartz fiber filter,
XAD-2 [270mg/1 40 mg])
10daysat25°C
or
30 days at 0°C.
TBD
Stable 30 days at
negative (-) 12°C
or at least 7 days
at 24°C.
10daysat25°Cor
30 days at 0°C.
TBD
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Osmium tetroxide,
6.1, Poison, UN2471
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
5600
(NIOSH)
10-3.1 /IO-3.4
(EPAORD)
5601
(NIOSH)
Not of concern in this sample type
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Store at < 4°C.
Store at < 4°C.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Store at < 4°C.
Store at < 4°C.
Store at < 4°C.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Phenol, solid, 6.1,
Poison, UN1671
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
TO-10A
(EPAORD)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Sample Collection Information Document
A1 -10
June 2010
-------
Air Samples
Analyte
Phorate sulfone
Phorate sulfoxide
Phosgene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl
phosphonic acid (PMPA)
(degradation product of
GD)<51
Propylene oxide151
Semivolatile organic
compounds, NOS
Sample
Volume'11
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
240 L (at 1
L/min)
240 - 7200 L
(at 1 - 5 L/min)
1 (at 0.3 ppm) -
16L
1 1 (at 0.5 ppm) -
100 L
240 - 7200 L
(at 1 - 5 L/min)
0.5 (at 100 ppm)
-5L
240 - 7200 L
(at 1 - 5 L/min)
Sample Container
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
4-mm i.d. x 6-mm o.d. x 1 1-cm
long silane-treated glass tubes
packed with 1 50 mg/75 mg of
pretreated XAD-2 adsorbent
coated with 2- HMP (2-
[hydroxymethyl] piperidine)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent tube (Hg(CN)2 coated
silica gel [300 mg/150 mg])
Bubbler (1 5 mLH2O)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Solid sorbent tube (coconut shell
charcoal [100 mg/50 mg])
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Holding Time
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Can be stored at
ambient
temperature for
up to 19 days.
Desorbed
samples remain
stable for at least
16 hours.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
7 days at 25°C.
TBD
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
TBD
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample
Preservation
or Preparation
Store at < 4°C.
Store at < 4°C.
Store at ambient
temperature.
Store at < 4°C.
Store at room
temperature.
TBD
Store at < 4°C.
Refrigerate
Store at < 4°C.
Packaging Requirements
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap each glass container
with bubble wrap. Pack samples as described in
Footnote (4). Air-tight container containing
activated carbon.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap each glass container
with bubble wrap. Pack samples as described in
Footnote (4). Air-tight container containing
activated carbon.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Phosgene, 2.3,
Poisonous gas,
UN1076
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Phosphine, 2.3,
Poisonous gas,
UN2199
Standard carrier
shipping label AND
Phosphorus
trichloride, 6.1,
Poison, UN1809
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable, UN1280.
Shipping label is
compound
dependent. May
require further
analysis.
Source/SAM
Method'31
TO-10A
(EPAORD)
TO-10A
(EPAORD)
OSHA61 /
Footnote (8)
TO-10A
(EPAORD)
6002
(NIOSH)
6402 (NIOSH)/
Footnote (8)
TO-10A
(EPAORD)
1612
(NIOSH)
TO-10A
(EPAORD)
Sample Collection Information Document
A1 -11
June 2010
-------
Air Samples
Analyte
Sodium arsenite (analyze for
total arsenic)151
Sodium azide (analyze as
azide ion)
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl pyrophosphate151
Tetramethylene-
disulfotetramine151
Thallium sulfate (analyze for
total thallium)
Thiodiglycol (TDG)
(degradation product of
HDf>
Thiofanox
1,4-Thioxane
(degradation product of HD)
Sample
Volume'11
Up to 1700m3
5 L (at 1 L/min)
Sample Container
Filter (quartz, silica, or cellulose
fiber)
Filter / Sorbent tube (PVC or
quartz filter with silica coated
sorbent [150 mg/75 mg])
Holding Time
6 months
Analyze samples
stored at room
temperature
within 10 days.
Sample
Preservation
or Preparation
None
Refrigerate when
not in transit.
Packaging Requirements
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
TBD
Shipping Label'2'
Standard carrier
shipping label AND
Sodium arsenite,
solid, 6.1, Poison,
UN2027
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
Source/SAM
Method'31
10-3.1 /IO-3.4
/ IO-3.5
(EPAORD)
ID-211
(OSHA)
Not of concern in this sample type
4 L (at 5 ppm) -
200 L
TBD
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Up to 1700m3
240 - 7200 L
(at 1 - 5 L/min)
12-240 L (at 0.1
- 1 .0 L/min)
Filter and treated filter (cellulose
and Na2CO3; preceded by 0.8-|jm
cellulose ester membrane)
Filter (quartz)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Filter (quartz, silica, or cellulose
fiber)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Filter / solid sorbent tube (OVS-2
tube: 13 mm quartz filter, XAD-2,
[270mg/140mg])
2 weeks
TBD
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
6 months
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
TBD
None
TBD
Store at < 4°C.
Store at < 4°C.
None
Store at < 4°C.
Store refrigerated
and tightly sealed.
Analyze at room
temperature.
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
TBD
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Place filter in protective covering. Wipe outside of
each sorbent tube clean using a damp, then dry
cloth. Cap both ends of the tube with plastic caps.
Place protected filter and/or tubes in double plastic
bags, and wrap with bubble wrap. Pack samples
as described in Footnote (4).
Standard carrier
shipping label AND
Sulfur dioxide, 2.3,
Poisonous gas,
UN1079
Standard carrier
shipping label AND
Sulfur trioxide, 6.1,
Poison, 8, Corrosive,
UN1829
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Pesticides, solid,
toxic, n.o.s, 6.1,
Poison, UN2588
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
solid, n.o.s, UN3335
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
6004
(NIOSH)
Method 8
(EPA OAQPS)
TO-10A
(EPAORD)
TO-10A
(EPAORD)
10-3.1 /IO-3.4/IO
3.5
(EPAORD)
TO-10A
(EPAORD)
5601
(NIOSH)
Not of concern in this sample type
Sample Collection Information Document
A1 -12
June 2010
-------
Air Samples
Analyte
Titanium tetrachloride
(analyze for total Titanium)
Triethanolamine (TEA)
(degradation product of HN-
3)(5>
Trimethyl phosphite151
1,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide
(analyze for total vanadium)
White phosphorus
Sample
Volume'11
Sample Container
Holding Time
Sample
Preservation
or Preparation
Packaging Requirements
Shipping Label'2'
Source/SAM
Method'31
Not of concern in this sample type
240 - 7200 L
(at 1 - 5 L/min)
240 - 7200 L
(at 1 - 5 L/min)
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Sorbent cartridge containing PUF
or PUF in combination with other
solid sorbent
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Store at < 4°C.
Store at < 4°C.
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Remove PUF cartridge from sampler, wrap with
aluminum foil used to store cartridge, and place in
a sealed container. Place container in double
plastic bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
TO-10A
(EPAORD)
TO-10A
(EPAORD)
Not of concern in this sample type
Not of concern in this sample type
Up to 1700m3
5 L (at 0.1
mg/m3) - 1 00 L
Filter (quartz, silica, or cellulose
fiber)
Solid sorbent tube (Tenax GC
[100mg/50mg])
6 months
7 days at 25°C.
None
None
Place filter in protective covering. Place protected
filter in double plastic bags, and wrap with bubble
wrap. Pack samples as described in Footnote (4).
Wipe outside of each sorbent tube clean using a
damp, then dry cloth. Cap tubes with plastic (not
rubber) caps. Place tubes in double plastic bags
and wrap with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381
10-3.1 /IO-3.4/IO
3.5
(EPAORD)
7905
(NIOSH)
Sample Collection Information Document
A1 -13
June 2010
-------
Aqueous/Liquid Samples
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)
Aldicarb sulfone
Aldicarb sulfoxide
Allyl alcohol
4-Aminopyridine
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
40 -160 ml
(one to four
VOA vials)
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Analyze samples
within 7 days of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Analyze samples
within 7 days of
collection.
Analyze samples
within 7 days of
collection.
Analyze samples
within 7 days of
collection.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Store at 4 (±2)°C.
Store at 4 (±2)°C.
Store at 4 (±2)°C.
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Acrylamide solution,
6.1, Poison, UN3426
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid,
6.1, Poison, UN1093
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Allyl alcohol, 3,
Flammable liquid,
6.1, Poison, UN1098.
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671
Source/SAM
Method'31
Adapted from
Chromatographia,
63(5/6): 223-237
Ch. 4/8316
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
MS014
(EPA CRL)
MS014
(EPA CRL)
MS014
(EPA CRL)
Ch. 4/5030C/
8260C
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
Sample Collection Information Document
A2-1
June 2010
-------
Aqueous/Liquid Samples
Analyte
Ammonia
Ammonium metavanadate
(analyze as total vanadium)
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Arsine'51
Asbestos
Boron trifluoride
Brodifacoum
Sample
Volume'11
0.5 -2 L
120 -480 ml
120 -480 ml
120 -480 ml
120 -480 ml
Sample Container
Polyethylene or glass container
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
Holding Time
Analyze within 28
days. Analyze
immediately if not
preserved.
Preserve with
acid within 2
weeks of
collection; can be
held 6 months
with acidification.
If not acid
preserved,
analyze
immediately.
Preserve with
acid within 2
weeks of
collection; can be
held 6 months
with acidification.
If not acid
preserved,
analyze
immediately.
Preserve with
acid within 2
weeks of
collection; can be
held 6 months
with acidification.
If not acid
preserved,
analyze
immediately.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Sample Preservation or
Preparation
Acidify to pH < 2 with
H2SO4 and refrigerate.
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (41.
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND (if
greater than 10%
ammonia by volume)
Ammonia solution, 8,
Corrosive. UN2672
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561
Standard carrier
shipping label AND
Arsine, 2.3 (2.1),
Poisonous gas,
UN2189
Source/SAM
Method'31
4500- NH3B/ 4500-
NH3G(SM)
200.7 / 200.8
(EPA OW)
200.7 / 200.8
(EPA OW)
200.7 / 200.8
(EPA OW)
200.7 / 200.8
(EPA OW)
Not of concern in this sample type
Not of concern in this sample type
1 -4L
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Ch.4/3520C/
3535A/8321B
(EPA SW-846)
Sample Collection Information Document
A2-2
June 2010
-------
Aqueous/Liquid Samples
Analyte
Bromadiolone'5'
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)
Carfentanil
Carbon disulfide
Chlorfenvinphos
Chlorine
Sample
Volume'11
1 -4L
120 -480 ml
1 -4L
1 -4L
40 -160 ml
(One to four
VOA vials)
1 -4L
0.5- 2 L
Sample Container
Amber glass container with PTFE-lined
septum or lid.
Plastic or glass (special cleaning
needed)'11'
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass bottle
Holding Time
Analyze samples
within 7 days of
collection.
Preserve with
acid within 2
weeks of
collection; can be
held 6 months
with acidification.
If not acid
preserved,
analyze
immediatelv.
Analyze samples
within 7 days of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Analyze
immediately
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Avoid excessive light and
agitation. Do not store
samples.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label
Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid,
UN1131.See
Footnote (1 0) when
bisulfate is used.
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Chlorine, 2.3,5.1,
Oxidizer, 8, Poison
gas, UN1017
Source/SAM
Method'31
MS014
(EPA CRL)
200.7 / 200.8
(EPA OW)
MS014
(EPA CRL)
Ch.4/3520C/
3535A/8321B
(EPA SW-846)
Ch. 4/5030C/
8260C
(EPA SW-846)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846
4500-CI G
(SM)
Sample Collection Information Document
A2-3
June 2010
-------
Aqueous/Liquid Samples
Analyte
2-Chloroethanol
3-Chloro-1,2-propanediol|s|
Chloropicrin151
Chlorpyrifos
Chlorpyrifos oxon
Crimidine151
Cyanide, Amenable to
chlorination
Sample
Volume'11
40 -160 ml
(One to four
VOA vials)
1 -4L
120 -240 ml
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
60-mL screw cap glass vials with PTFE-
lined septum or lid.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Glass or polyethylene bottle (1-L size)
Holding Time
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract all
samples within 14
days of collection
and analyze
within 14 days
following
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
14 days
Sample Preservation or
Preparation
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to 4°C and store
extracts at < negative (-)
10°C until analysis.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Add NaOH until pH > 12
at time of collection; cool
to 4°C.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label. See
Footnote (1 0) when
bisulfate is used.
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label AND
Cyanide solutions
n.o.s, 6.1, Poison,
UN1935
Source/SAM
Method'31
Ch. 4/5030C/
8260C
(SW-846)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
551.1
(EPA OW)
Ch.4/3520C/
3535A / 8270D
(EPA SW-846)
Ch.4/3520C/
3535A / 8270D
(EPA SW-846)
Ch.4/3535A/
8270D
(EPA SW-846)
3135.21
(EPA RLAB)
Sample Collection Information Document
A2-4
June 2010
-------
Aqueous/Liquid Samples
Analyte
Cyanide, Total
Cyanogen chloride151
1,2-Dichloroethane
(degradation product of HD)
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl
methylphosphonate (DIMP)
(degradation product of
GB)(5>
Sample
Volume'11
0.5 -2 L
40 -160 ml
(one to four
VOA vials)
40 -160 ml
(one to four
VOA vials)
1 -4L
1 -4L
1 -4L
TBD
Sample Container
Glass or polyethylene bottle
40-mL VOA glass screw-cap vials with
PTFE septa
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
1 80 days
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Analyze samples
within 1 day of
collection.
Sample Preservation or
Preparation
Acidify to pH < 2 with
nitric acid immediately
after collection; cool to 4
(±2)°C from time of
collection until time of
digestion.'12'
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Store at 4 (±2)°C.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Cyanide solutions
n.o.s, 6.1, Poison,
UN1580
Standard carrier
shipping label AND
Cyanogen chloride,
2.3, Poisonous gas,
UN1589. See
Footnote (1 0) when
bisulfate is used.
Standard carrier
shipping label AND
Ethylene dichloride.
3, Flammable liquid,
6, Poison, UN1184.
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label
Source/SAM
Method'31
ILM05.3CN
(EPA CLP)
Ch. 4/5030C/
8260C
(EPA SW-846) /
Footnote (8)
Ch. 4/5030C/
8260C
(EPA SW-846)
Ch.4/3535A/
8270D
(EPA SW-846)
Ch.4/3535A/
8270D
(EPA SW-846)
Ch. 4/3520C/
3535A/8015C
(EPA SW-846)
MS017
(EPA CRL)
Sample Collection Information Document
A2-5
June 2010
-------
Aqueous/Liquid Samples
Analyte
Dimethylphosphite'51
Dimethylphosphoramidic
acid (degradation product
GA)'5'
Diphacinone
Disulfoton
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of
HD)<5>
EA2192
[Diisopropylaminoethyl
methylthiolophosphonate]
(hydrolysis product of VX)(S|
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract
immediately after
collection and
preservation and
store at 4 C.
Analyze extracts
within 30 days.
Extract
immediately after
collection and
preservation and
store at 4°C.
Analyze extracts
within 30 days.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Sample Preservation or
Preparation
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.*9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Cool to 4°C. Add sodium
sulfite to remove residual
chlorine from treated
wastewater samples and
then adicify to pH < 2
with 6N HCI.(9)
Cool to 4°C. Add sodium
sulfite to remove residual
chlorine from treated
wastewater samples and
then adicify to pH < 2
with 6N HCI.(9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. <9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.*9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
TBD(7)
Air-tight container containing
activated carbon
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
TBD(7)
Source/SAM
Method'31
Ch. 4/3535A/
8270D
(EPA SW-846) /
Footnote (8)
Ch. 4/3535A/
8321 B
(EPA SW-846)
Ch.4/3520C/
3535A/8321B
(EPA SW-846)
525.2
(EPA OW)
525.2
(EPA OW)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8321 B
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A2-6
June 2010
-------
Aqueous/Liquid Samples
Analyte
Ethyl methylphosphonic acid
(EMPA)
(degradation product of
vxf
Ethyldichloroarsine (ED)'51
N-Ethyldiethanolamine
(EDEA) (degradation product
of HN-1)'51
Ethylene oxide
Fenamiphos151
Fentanyl
Fluoride
Fluoroacetamide
Sample
Volume'11
1 -4L
1 -4L
1 -4L
40 -160 ml
(one to four
VOA vials)
1 -4L
1 -4L
40 ml (one
VOA vial)
TBD
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA vial
TBD
Holding Time
Analyze samples
within 1 day of
collection.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Analyze samples
within 7 days of
collection.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
28 days
TBD
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Store at 4 (±2)°C.
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
None
TBD
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
TBD
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Ethyldichloroarsine,
6.1, Poison, UN1892
Standard carrier
shipping label
Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to
40CFR172.101 for
more information).
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label
Source/SAM
Method'31
MS017
(EPA CRL)
Ch. 4/3535A/
8270D
(EPA SW-846)
MS016
(EPA CRL)
Ch. 4/5030C/
8260C
(EPA SW-846)
Ch.4/3520C/
3535A / 8270D
(EPA SW-846)
Ch.4/3520C/
3535A/8321B
(EPA SW-846)
300.1, Rev 1.0
(EPA OW)
Adapted from
Journal of
Chromatography B,
876(1): 103-108
Sample Collection Information Document
A2-7
June 2010
-------
Aqueous/Liquid Samples
Analyte
Fluoroacetic acid and
fluoroacetate salts (analyze
for fluoroacetate ion)151
2-Fluoroethanol
Formaldehyde
Gasoline Range Organics
Hexahydro-1,3,5-trinitro-1,3,5
triazine (RDX)
Hexamethylenetriperoxide-
diamine (HMTD)'51
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide15'
Hydrogen fluoride
Hydrogen sulfide
Sample
Volume'11
40 ml (one
VOA vial)
100 -400 ml
100 -400 ml
40 -160 ml
(One to four
VOA vials)
1 -4L
1 -4L
Sample Container
40-mL VOA vial
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
28 days
14 days
Derivatized and
extracted within 3
days of collection.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
None
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples. <9)
Refrigerate at 4°C.
Preserve to pH 5.0 to
minimize the formation of
artifact formaldehyde.
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples. (9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label'21
Standard carrier
shipping label AND
Fluoroacetic acid,
6.1, Poison, UN2642
Standard carrier
shipping label
Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to
40CFR172.101 for
more information).
Standard carrier AND
Gasoline
identification set, 2.3,
Poison gas, NA9035
shipping label. See
Footnote (1 0) when
bisulfate is used.
Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483
Forbidden
Source/SAM
Method'31
300.1, Rev 1.0
(EPA OW)
Ch. 4/5030C/
8260C
(SW-846)
Ch.4/8315A
(EPA SW-846)
Ch. 4/5030C/
801 5C
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Sample Collection Information Document
A2-8
June 2010
-------
Aqueous/Liquid Samples
Analyte
Isopropyl methylphosphonic
acid (IMPA) (degradation
product of GB)'51
Kerosene'51
Lead arsenate
(analyze for total arsenic)
Lewisite oxide
(degradation product of
Lewisite)'51
Mercuric chloride (analyze
for total mercury)
Mercury, Total
Methamidophos
Sample
Volume'11
1 -4L
1 -4L
120 -480 ml
120 -480 ml
400 ml
400 ml
TBD
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
PTFE, plastic or glass
PTFE, plastic or glass
TBD
Holding Time
Analyze samples
within 1 day of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Preserve with
acid within 2
weeks of
collection; can be
held 6 months
with acidification.
If not acid
preserved,
analyze
immediately.
Samples should
be preserved with
acid within 2
weeks of
collection, and
can be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
28 days
28 days
TBD
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Acidify to pH < 2 with
nitric acid.
Acidify to pH < 2 with
nitric acid.
TBD
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal
the container with non-reactive tape
or film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
TBD
Shipping Label'21
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
Standard carrier
shipping label AND
Arsenic compounds,
liquid, n.o.s, 6.1,
Poison, UN1556
Standard carrier
shipping label AND
Mercuric chloride,
6.1, Poison, UN1624
Standard carrier
shipping label AND
Mercury, 8,
Corrosive, UN2809
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
MS017
(EPA CRL)
Ch. 4/5030C/
801 5C
(EPA SW-846)
200.7 / 200.8
(EPA OW)
200.7 / 200.8
(EPA OW) /
Footnote (8)
Ch. 3 / 7473
(EPA SW-846)
Ch. 3 / 7473
(EPA SW-846)
Adapted from
Chromatographia,
63(5/6): 233-237
Sample Collection Information Document
A2-9
June 2010
-------
Aqueous/Liquid Samples
Analyte
Methomyl
Methoxyethylmercuric
acetate (analyze for total
mercury)151
Methyl acrylonitrile
Methyl fluoroacetate
(analyze for fluoroacetate
ion)'51
Methyl hydrazine151
Methyl isocyanate
Methyl paraoxon
Methyl parathion
Methylamine
N-Methyldiethanolamine
(MDEA) (degradation
product of HN-2)(S|
Sample
Volume'11
1 -4L
400 ml
1 -4L
40 ml (one
VOA vial)
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
PTFE, plastic or glass
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA vial
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Analyze samples
within 7 days of
collection.
28 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
28 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Acidify to pH < 2 with
nitric acid
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
None
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label'21
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Mercury compounds,
liquid, n.o.s, 6.1,
Poison, UN2024
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
Standard carrier
shipping label AND
Methyl hydrazine,
6.1, Poison, UN1244
Source/SAM
Method'31
MS014
(EPA CRL)
Ch. 3 / 7473
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
300.1, Rev 1.0
(EPA OW)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
1 -4L
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. <9)
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Analyze samples
within 7 days of
collection.
Store at 4 (±2)°C.
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label
MS016
(EPA CRL)
Sample Collection Information Document
A2-10
June 2010
-------
Aqueous/Liquid Samples
Analyte
Methylphosphonic acid
(MPA)
(degradation product of VX,
GB, & GD)(S|
Mevinphos
Monocrotophos
Nicotine compounds
(analyze as nicotine)
Octahydro-1,3,5,7-tetranitro-
1,3,5,7-tetrazocine (HMX)
Organophosphate
pesticides, NOS
Osmium tetraoxide (analyze
for total osmium)151
Oxamyl
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
20 - 80 ml
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Plastic or glass (special cleaning
needed)01'
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Analyze samples
within 1 day of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
6 months
Analyze samples
within 7 days of
collection.
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples.'9'
Cool to 4°C.
Preserve samples to pH
< 2 with HCI upon
collection.'12'
Store at 4 (±2)°C.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Nicotine compounds,
liquid, n.o.s, 6.1,
Poison, UN3144
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Osmium tetroxide,
6.1, Poison, UN2471
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Source/SAM
Method'31
MS017
(EPA CRL)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
614
(EPA OW)
252.2
(EPA OW)
MS014
(EPA CRL)
Sample Collection Information Document
A2-11
June 2010
-------
Aqueous/Liquid Samples
Analyte
Paraquat
Paraoxon
Parathion
Pentaerythritol tetranitrate
(PETN)
Phencyclidine
Phenol
Phorate
Phorate sulfone
Sample
Volume'11
250-1000
ml
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
Polyvinylchloride (PVC) high-density or
silanized amber glass bottle fitted with
screw caps
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within 21
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
Refrigerate at 4°C from
time of collection until
extraction. Add 100
mg/L sodium thiosulfate
to samples containing
residual chlorine. Keep
protected from light.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.*9'
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples. (9)
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples. <9)
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples. <9)
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples. <9)
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Bipyridilium
pesticides, liquid, 6.1,
Toxic, UN3016
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Forbidden
Standard carrier
shipping label
Standard carrier
shipping label AND
Phenol solutions, 6.1,
Poison, UN2821
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
549.2
(EPA OW)
Ch. 4/3520C/
3535A / 8270D
(SW-846)
Ch. 4/3520C/
3535A / 8270D
(SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Sample Collection Information Document
A2-12
June 2010
-------
Aqueous/Liquid Samples
Analyte
Phorate sulfoxide
Phosqene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl phosphonic
acid (PMPA) (degradation
product of GD)'51
Propylene oxide151
Semivolatile organic
compounds, NOS
Sodium arsenite (analyze for
total arsenic)151
Sodium azide (analyze as
azide ion)
Sample
Volume'11
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
Ch. 4/3535A/
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples. (9)
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
TBD
40 -160 ml
(one to four
VOA vials)
1 -4L
120 -480 ml
40 ml (one
VOA vial)
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
40-mL VOA glass screw-cap vials with
PTFE septa
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Plastic or glass (special cleaning
needed)'11'
40-mL VOA vial
Analyze samples
within 1 day of
collection.
14 days
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Samples should
be preserved with
acid within 2
weeks of
collection, and
can be held 6
28 days
Store at 4 (±2)°C.
Cool to 4°C and adjust to
pH < 2 with H2SO4, HCI,
or solid NaHSO4. Store
samples in capped vials,
with no headspace in an
area free of solvent
fumes. Add sodium
thiosulfate to remove
residual chlorine from
treated wastewater
samples.*9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
None
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable liquid,
UN1280. See
Footnote (1 0) when
bisulfate is used.
Shipping label is
compound
dependent. May
require further
analysis.
Standard carrier
shipping label AND
Sodium arsenite,
aqueous solutions,
6.1, Poison, UN1686
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
MS017
(EPA CRL)
Ch. 4/5030C/
8260C
(EPA SW-846)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
200.7 / 200.8
(EPA OW)
Adapted from J. of
Forensic Sciences,
43(1): 200-202 /
300.1, Rev 1.0
(EPA OW)
Sample Collection Information Document
A2-13
June 2010
-------
Aqueous/Liquid Samples
Analyte
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl pyrophosphate151
Tetramethylene-
disulfotetramine151
Thallium sulfate (analyze for
total thallium)
Thiodiglycol (TDG)
(degradation product of
HD)<5>
Thiofanox
1,4-Thioxane
(degradation product of
HD)<5>
Titanium tetrachloride
(analyze for total titanium)
Sample
Volume'11
1 -4L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Sample Preservation or
Preparation
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Strychnine, 6.1,
Poison, UN1692
Source/SAM
Method'31
Ch. 4/3535A/
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 -4L
1 -4L
120 -480 ml
1 -4L
1 -4L
1 -4L
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Plastic or glass (special cleaning
needed)'11'
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Samples should
be preserved with
acid within 2
weeks of
collection, and
can be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Analyze samples
within 7 days of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Pesticides, liquid,
toxic, n.o.s, 6.1,
Poison, UN2902
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
liquid, n.o.s, UN3334
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3520C/
3535A / 8270D
(EPA SW-846)
200.7 / 200.8
(EPA OW)
MS015
(EPA CRL)
Ch. 4/3520C/
3535A/8321B
(EPA SW-846)
Ch. 4/3535A/
8270D
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A2-14
June 2010
-------
Aqueous/Liquid Samples
Analyte
Triethanolamine (TEA)
(degradation product of HN-
3)(5>
Trimethyl phosphite151
1,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide
(analyze for total vanadium)
White phosphorus
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
120 -480 ml
0.5 -2 L
Sample Container
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Amber glass or PTFE container with
PTFE-lined septum or lid. When PTFE-
lined septa or lids are not available,
solvent-rinsed aluminum foil may be
used as a liner.
Plastic or glass (special cleaning
needed)'11'
Wide-mouth glass or PTFE container
with PTFE-lined septum or lid; fill to
overflowing to eliminate headspace
Holding Time
Analyze samples
within 7 days of
collection.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Extract samples
within 7 days of
collection;
analyze within 40
days of
extraction.
Samples should
be preserved with
acid within 2
weeks of
collection, and
can be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Extracts with
isooctane should
be analyzed
within 30 days.
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add
sodium thiosulfate to
remove residual chlorine
from wastewater
samples.'9'
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples.'9'
Cool to < 6°C and store
in the dark. Add sodium
thiosulfate to remove
residual chlorine from
wastewater samples.'9'
Acidify to pH < 2 with
nitric acid and store at
4°C. If arsenic species
are to be determined,
remove unpreserved
aliquot prior to sample
preservation.'12'
Cool to 4°C and store in
the dark.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381
Source/SAM
Method'31
MS016
(EPA CRL)
Ch. 4/3535A/
8270D
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
Ch. 4/3535A/
8330B
(EPA SW-846)
200.7 / 200.8
(EPA OW)
7580
(EPA SW-846)
Sample Collection Information Document
A2-15
June 2010
-------
Drinking Water Samples
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)
Aldicarb sulfone
Aldicarb sulfoxide
Allyl alcohol
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
40 -160 ml
(one to four
VOA vials)
Sample Container
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
40-mL VOA glass screw-cap vials
with PTFE septa
Holding Time
Analyze
samples within 7
days of
collection.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
28 days
28 days
28 days
14 days
Sample Preservation or
Preparation
Store at 4 (±2)°C.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Cool to 4°C and adjust to pH <
2withH2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Acrylamide solution,
6.1, Poison, UN3426
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid,
6.1, Poison, UN1093
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Allyl alcohol, 3,
Flammable liquid,
6.1, Poison, UN1098.
See Footnote (1 0)
when bisulfate is
used.
Source/SAM
Method'31
Adapted from
Chromatographia,
65(5/6): 233-237
Ch. 4/8316
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
531.2
(EPA OW)
531.2
(EPA OW)
531.2
(EPA OW)
Ch. 4 / 5030C /
8260C
(EPA SW-846)
Sample Collection Information Document
A3-1
June 2010
-------
Drinking Water Samples
Analyte
4-Aminopyridine
Ammonia
Ammonium metavanadate
(analyze as total vanadium)
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Sample
Volume'11
1 -4L
400-1600
ml
120-480
ml
120-480
ml
120-480
ml
Sample Container
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Pastic or glass bottles
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
Plastic or glass (special cleaning
needed)'11'
Holding Time
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
28 days
Preserve with
acid within 2
weeks of
collection; can
be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Preserve with
acid within 2
weeks of
collection.
Samples can be
held 6 months
with
acidification; if
not acid
preserved,
analyze
Preserve with
acid within 2
weeks of
collection; can
be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Sample Preservation or
Preparation
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Cool to 4°C and adjust to pH <
2 with H2SO4. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671
Standard carrier
shipping label AND (if
greater than 10%
ammonia by volume)
Ammonia solution, 8,
Corrosive, UN2672
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561
Source/SAM
Method'31
Ch. 4 / 3535A /
8330B
(EPA SW-846)
350.1
(EPA OW)
200.7 / 200.8
(EPA OW)
200.7/200.8
(EPA OW)
200.7 / 200.8
(EPA OW)
Sample Collection Information Document
A3-2
June 2010
-------
Drinking Water Samples
Analyte
Arsine'51
Asbestos
Boron trifluoride
Brodifacoum
Bromadiolone151
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)
Carfentanil
Sample
Volume'11
120-480
ml
Sample Container
Plastic or glass (special cleaning
needed)01'
Holding Time
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Sample Preservation or
Preparation
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Arsine, 2.3(2.1),
Poisonous gas,
UN2189
Source/SAM
Method'31
200.7 / 200.8
(EPA OW)
Not of concern in this sample type
Not of concern in this sample type
1 -4L
1 -4L
120-480
ml
25 -100 ml
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with screw-caps with
PTFE-lined septa or lids. If PTFE
is not available, solvent rinsed
aluminum foil may be used as a
liner.
Plastic or glass (special cleaning
needed)'11'
Amber 40- or 60-mL glass bottles
with PTFE-lined screw caps.
Preservative should be added to
bottle before sample collection.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Preserve with
acid within 2
weeks of
collection; can
be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
28 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label
Ch.4 / 3520C /
3535A / 8321 B
(EPA SW-846)
Ch. 4 / 3520C /
3535A / 8321 B
(EPA SW-846)
200.7 / 200.8
(EPA OW)
531.2
(EPA OW)
Ch. 4 / 3520C /
3535A / 8321 B
(EPA SW-846)
Sample Collection Information Document
A3-3
June 2010
-------
Drinking Water Samples
Analyte
Carbon disulfide
Chlorfenvinphos
Chlorine
2-Chloroethanol
3-Chloro-1,2-propanediol'51
Chloropicrin151
Sample
Volume'11
25 -100 ml
1 -4L
0.5 -2 L
40 -160 ml
(one to four
VOA vials)
1 -4L
120-240
ml
Sample Container
40-mL to 120-mL screw cap vials
each equipped with a PTFE-lined
silicone septum (Special cleaning
required). <13) Fill sample bottles to
overflowing.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass bottle
40-mL VOA glass screw-cap vials
with PTFE septa
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
60-mL screw cap glass vials with
PTFE-lined septum or lid.
Holding Time
14 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Analyze
immediately.
14 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract all
samples within
14 days of
collection and
analyze within
14 days
following
extraction.
Sample Preservation or
Preparation
If residual chlorine is present,
add ~25 mg of ascorbic acid per
40 ml of sample to the sample
bottle before filling. Adjust to
pH < 2 at time of collection, but
after dechlorination, with 2
drops of 1:1 HCI for each 40 ml
of sample. Store samples at £
4°C until analysis. The sample
storage area must be free of
organic solvent vapors and
direct or intense light.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Avoid excessive light and
agitation.
Cool to 4°C and adjust to pH <
2 with H2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated
wastewater samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to 4°C and store extracts
at < negative (-) 10°C until
analysis.
Packaging Requirements
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Shipping Label121
Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid,
UN1131.See
Footnote (10) when
bisulfate is used.
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Chlorine, 2.3, 5.1,
Oxidizer, 8, Poison
gas, UN1017
Standard carrier
shipping label. See
Footnote (10) when
bisulfate is used.
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Source/SAM
Method'31
524.2
(EPA OW)
Ch. 4 / 3520C /
3535A / 8270D
(EPA SW-846)
4500-CI G
(SM)
Ch. 4 / 5030C /
8260C
(EPA SW-846)
Ch. 4 / 3520C/
3535A / 8270D
(EPA SW-846)
551.1
(EPA OW)
Sample Collection Information Document
A3-4
June 2010
-------
Drinking Water Samples
Analyte
Chlorpyrifos
Chlorpyrifos oxon
Crimidine151
Cyanide, Amenable to
chlorination
Cyanide, Total
Cyanogen chloride151
1,2-Dichloroethane
(degradation product of HD)
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
50 - 200 ml
40 -160 ml
(one to four
VOA vials)
25 -100 ml
Sample Container
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Glass or polyethylene bottle (1-L
size)
Plastic or glass
40-mL VOA glass screw-cap vials
with PTFE septa
40-mL to 120-mL screw cap vials
each equipped with a PTFE-lined
silicone septum (special cleaning
required). (13) Fill sample bottles to
overflowing.
Holding Time
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
14 days
14 days
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
14 days
Sample Preservation or
Preparation
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Add NaOH until pH > 12 at time
of collection; cool to 4°C.
Adjust to pH a 12 with sodium
hydroxide; cool to 4°C at time of
collection. If feasible, analyze
samples immediately upon
receipt at laboratory.
Cool to 4°C and adjust to pH <
2withH2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
If residual chlorine is present
add ~25 mg of ascorbic acid per
40 ml of sample to the sample
bottle before filling. Adjust to
pH < 2 at time of collection, but
after dechlorination, with 2
drops of 1:1 HCI for each 40 ml
of sample. Seal the sample
bottle.
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4). (Air-tight container
containing activated carbon)
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label AND
Cyanide solutions
n.o.s, 6.1, Poison,
UN1935
Standard carrier
shipping label AND
Cyanide solutions
n.o.s, 6.1, Poison,
UN1580
Standard carrier
shipping label AND
Cyanogen chloride,
2.3, Poisonous gas,
UN1589. See
Footnote (10) when
bisulfate is used.
Standard carrier
shipping label AND
Ethylene dichloride.
3, Flammable liquid,
6, Poison, UN1184.
See Footnote (1 0)
when bisulfate is
used.
Source/SAM
Method'31
Ch.4 /3520C/
3535A / 8270D
(EPA SW-846)
Ch.4 /3520C/
3535A / 8270D
(EPA SW-846)
3535A / 8270D
(EPA SW-846)
3135.21
(EPA RLAB)
335.4
(EPA OW)
Ch. 4 / 5030C /
8260C
(EPA SW-846) /
Footnote (8)
524.2
(EPA OW)
Sample Collection Information Document
A3-5
June 2010
-------
Drinking Water Samples
Analyte
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl
methylphosphonate (DIMP)
(degradation product of
GB)<51
Dimethylphosphite151
Dimethylphosphoramidic
acid (degradation product
GAf
Diphacinone
Disulfoton
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
1-L or 1-quart amber glass
bottles fitted with PTFE-lined
screw caps (amber bottles are
preferred)
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
1-L or 1-quart amber glass
bottles fitted with PTFE-lined
screw caps (amber bottles are
preferred)
Holding Time
Extract samples
within 14 days of
collection;
analyze within
30 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 14 days of
collection;
analyze within
30 days of
extraction.
Sample Preservation or
Preparation
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.(9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated water
samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.(9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid,
toxic, 6.1, Poison,
UN3023
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
525.2
(EPA OW)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3520C /
3535A/8015C
(EPA SW-846)
Ch. 4 / 3535A /
832 1B
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3535A /
832 1B
(EPA SW-846)
Ch. 4 / 3520C /
3535A / 8321 B
(EPA SW-846)
525.2
(EPA OW)
Sample Collection Information Document
A3-6
June 2010
-------
Drinking Water Samples
Analyte
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of
HD)<51
EA2192
[Diisopropylaminoethyl
methyl thiolophosphonate]
(hydrolysis product of VX)(S|
Ethylmethyl phosphonate
(EMPA)
(degradation product of
VX)'5'
Ethyldichloroarsine (ED)(S|
N-Ethyldiethanolamine
(EDEA) (degradation product
of HN-1)(S|
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
1-L or 1-quart amber glass
bottles fitted with PTFE-lined
screw caps (amber bottles are
preferred)
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection;
analyze within
30 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.(9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
TBD(7)
Air-tight container containing
activated carbon
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
TBD(7)
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Ethyldichloroarsine,
6.1, Poison, UN1892
Standard carrier
shipping label
Source/SAM
Method'31
525.2
(EPA OW)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3535A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3535A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
3520C / 3535A /
832 1B
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A3-7
June 2010
-------
Drinking Water Samples
Analyte
Ethylene oxide
Fenamiphos
Fentanyl
Fluoride
Fluoroacetamide
Fluoroacetic acid and
fluoroacetate salts (analyze
for fluoroacetate ion)151
2-Fluoroethanol
Sample
Volume'11
40 -160 ml
(one to four
VOA vials)
1 -4L
1 -4L
40 ml (one
VOA vial)
TBD
60 uL
100-400
ml
Sample Container
40-mL VOA glass screw-cap vials
with PTFE septa
Amber glass bottles fitted with
PTFE-lined screw caps
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
40-mL VOA vial
TBD
Plastic (HOPE) or glass bottles
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
14 days
Extract
immediately;
extracts may be
stored for up to
30 days after
sample
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
28 days
TBD
28 days
14 days
Sample Preservation or
Preparation
Cool to 4°C and adjust to pH <
2 with H2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.(9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
None
TBD
None
Cool to 4°C and adjust to pH <
2withH2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated water
samples. (9)
Packaging Requirements
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
TBD
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to
40CFR 172.101 for
more information).
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label
Source/SAM
Method'31
Ch. 4 / 5030C /
8260C
(EPA SW-846)
525.2
(EPA OW)
3520C / 3535A /
832 1B
(EPA SW-846)
300.1, Rev 1.0
(EPA OW)
Adapted from
Journal of
Chromatography B,
876(1): 103-108
300.1, Rev 1.0
(EPA OW)
5030C / 8260C
(EPA SW-846)
Sample Collection Information Document
A3-8
June 2010
-------
Drinking Water Samples
Analyte
Formaldehyde
Gasoline Range Organics
Hexahydro-1,3,5-trinitro-
1,3,5-triazine(RDX)
Hexamethylenetriperoxide-
diamine (HMTD)'51
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide151
Hydrogen fluoride
Hydrogen sulfide
Isopropyl methylphosphonic
acid (IMPA) (degradation
product of GB)'51
Kerosene'51
Sample
Volume'11
100-400
ml
40 -160 ml
(One to four
VOA vials)
1 -4L
1 -4L
Sample Container
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
40-mL VOA glass screw-cap vials
with PTFE septa
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Derivatize and
extract within 3
days of
collection.
14 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Buffer to pH 5.0 and store at
4°C.
Cool to 4°C and adjust to pH <
2 with H2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Packaging Reguirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label'21
Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to
40CFR 172.101 for
more information).
Standard carrier AND
Gasoline
identification set, 2.3,
Poison gas, NA9035.
See Footnote (1 0)
when bisulfate is
used.
Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483
Forbidden
Source/SAM
Method'31
831 5A
(EPA SW-846)
Ch. 4 / 5030C /
801 5C
(EPA SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
1 -4L
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
14 days
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to 4°C and adjust to pH <
2withH2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (1 0)
when bisulfate is
used.
Ch. 4 / 3535A /
832 1B
(EPA SW-846)
5030C/8015C
(EPA SW-846)
Sample Collection Information Document
A3-9
June 2010
-------
Drinking Water Samples
Analyte
Lead arsenate
(analyze for total arsenic)
Lewisite oxide
(degradation product of
Lewisite)'51
Mercuric chloride (analyze
for total mercury)
Mercury, Total
Methamidophos
Methomyl
Sample
Volume'11
120-480
ml
120-480
ml
100-400
ml
100-400
ml
TBD
25 -100 ml
Sample Container
Plastic or glass (special cleaning
needed)01'
Plastic or glass (special cleaning
needed)'11'
BOD bottle or ground glass
stoppered flask
BOD bottle or ground glass
stoppered flask
TBD
Amber 40- or 60-mL glass bottles
with PTFE-lined screw caps.
Preservative should be added to
bottle before sample collection.
Holding Time
Preserve with
acid within 2
weeks of
collection; can
be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Preserve with
acid within 2
weeks of
collection.
Samples can be
held 6 months
with
acidification; if
not acid
preserved,
analyze
28 days
28 days
TBD
28 days
Sample Preservation or
Preparation
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Preserve samples to pH < 2
with nitric acid upon collection
and hold for 16 hours before
analyzing. If dissolved mercury
is to be determined, filter the
sample prior to preservation.'12'
Preserve samples to pH < 2
with nitric acid upon collection
and hold for 16 hours before
analyzing. If dissolved mercury
is to be determined, filter the
sample prior to preservation.'12'
TBD
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
TBD
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label'21
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
Standard carrier
shipping label AND
Arsenic compounds,
liquid, n.o.s, 6.1,
Poison, UN1556
Standard carrier
shipping label AND
Mercuric chloride,
6.1, Poison, UN1624
Standard carrier
shipping label AND
Mercury, 8,
Corrosive, UN2809
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Source/SAM
Method'31
200.7 / 200.8
(EPA OW)
200.7 / 200.8
(EPA OW) /
Footnote (8)
245.1
(EPA OW)
245.1
(EPA OW) /
40 CFR 136.3
Adapted from
Chromatographia,
63(5/6): 233-237
531.2
(EPA OW)
Sample Collection Information Document
A3-10
June 2010
-------
Drinking Water Samples
Analyte
Methoxyethylmercuric
acetate (analyze for total
mercury)151
Methyl acrylonitrile
Methyl fluoroacetate
(analyze for fluoroacetate
ion)151
Methyl hydrazine151
Methyl isocyanate
Methyl paraoxon
Methyl parathion
Methylamine
N-Methyldiethanolamine
(MDEA) (degradation
product of HN-2)'51
Methylphosphonic acid
(MPA)
(degradation product of VX,
GB, & GD)'51
Sample
Volume'11
100-400
ml
1 -4L
40 ml (one
VOA vial)
1 -4L
Sample Container
PTFE or glass
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
40-mL VOA vial
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
28 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
28 days
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Preserve samples to pH < 2
with nitric acid upon collection.
If mercury species are to be
determined, remove a sample
aliquot prior to preservation. <12)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated water
samples. (9)
None
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Mercury compounds,
liquid, n.o.s, 6.1,
Poison, UN2024
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
Standard carrier
shipping label AND
Methyl hydrazine,
6.1, Poison, UN1244
Source/SAM
Method'31
245.1
(EPA OW) /
40 CFR 136.3
Ch. 4/8316
(EPA SW-846)
300.1, Rev 1.0
(EPA OW)
Ch. 4 / 3520C /
3535A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
3535A / 8270D
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Ch. 4 / 3520C /
3535A / 8321 B
(EPA SW-846)
Ch. 4 / 3535A /
832 1B
(EPA SW-846)
Sample Collection Information Document
A3-11
June 2010
-------
Drinking Water Samples
Analyte
Mevinphos
Monocrotophos
Nicotine compounds
(analyze as nicotine)
Octahydro-1,3,5,7-tetranitro-
1,3,5,7-tetrazocine(HMX)
O rganophosphate
pesticides, NOS
Osmium tetraoxide (analyze
for total osmium)151
Oxamyl
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
25 -100 ml
Sample Container
Amber glass bottles fitted with
PTFE-lined screw caps
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Samples must be collected in
glass containers and should not
be prerinsed before sampling
Plastic or glass (special cleaning
needed)'11'
Amber 40- or 60-mL glass bottles
with PTFE-lined screw caps.
Preservative should be added to
bottle before sample collection.
Holding Time
Extract samples
within 14 days of
collection;
analyze within
30 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 14 days of
collection;
analyze within
1 4 - 28 days of
extraction
(depending on
the analyte).
6 months
28 days
Sample Preservation or
Preparation
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.<9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C and store in the
dark. Add sodium thiosulfate to
remove residual chlorine from
treated drinking water
samples.'9'
If residual chlorine is present,
add 80 mg of sodium thiosulfate
per liter of sample. Ice or
refrigerate at 4°C away from
light from the time of collection
until and after extraction.
Acidify to pH < 2 with HCL.(12)
Preserve with potassium
dihydrogen citrate and sodium
thiosulfate (if residual chlorine is
present)'9'. After collecting the
sample, cap carefully, and
agitate for 1 minute. Store at <
6°C and protect from light until
analysis. Do not freeze.
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label'21
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Nicotine compounds,
liquid, n.o.s, 6.1,
Poison, UN3144
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Osmium tetroxide,
6.1, Poison, UN2471
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Source/SAM
Method'31
525.2
(EPA OW)
3535A / 8270D
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
507
(EPA OW)
252.2
(EPA OW) /
40 CFR 136.3
531.2
(EPA OW)
Sample Collection Information Document
A3-12
June 2010
-------
Drinking Water Samples
Analyte
Paraquat
Paraoxon
Parathion
Pentaerythritol tetranitrate
(PETN)
Phencyclidine
Phenol
Phorate
Phorate sulfone
Sample
Volume'11
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
1 -4L
Sample Container
High density PVC or silanized
amber glass bottle (1-L) fitted
with screw cap.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within
21 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Refrigerate at 4°C from time of
collection until extraction. Add
100 mg/L sodium thiosulfate to
samples containing residual
chlorine. Keep protected from
light.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from wastewater
samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from wastewater
samples. (9)
Cool to < 6°C and store in the
dark. Add sodium thiosulfate to
remove residual chlorine from
treated drinking water
samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Bipyridilium
pesticides, liquid, 6.1,
Toxic, UN3016
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Forbidden
Standard carrier
shipping label
Standard carrier
shipping label AND
Phenol solutions, 6.1,
Poison, UN2821
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
549.2
(EPA OW)
3520C / 3535A /
8270D
(EPA SW-846)
3520C / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3520C /
3535A / 8270D
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
3535A / 8270D
(EPA SW-846)
Sample Collection Information Document
A3-13
June 2010
-------
Drinking Water Samples
Analyte
Phorate sulfoxide
Phosgene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl phosphonic
acid (PMPA) (degradation
product of GD)'51
Propylene oxide151
Semivolatile organic
compounds, NOS
Sodium arsenite (analyze for
total arsenic)151
Sample
Volume'11
1 -4L
Sample Container
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Source/SAM
Method'31
3535A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from wastewater
samples. (9)
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
3520C / 3535A /
82701 D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 -4L
40 -160 ml
(One to four
VOA vials)
1 -4L
120-480
ml
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
40-mL VOA glass screw-cap vials
with PTFE septa
Amber glass bottles fitted with
PTFE-lined screw caps
Plastic or glass (special cleaning
needed)01'
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
14 days
Extract samples
within 14 days of
collection;
analyze within
30 days of
extraction.
Preserve with acid
within 2 weeks of
collection.
Samples can be
held 6 months
with acidification;
if not acid
preserved,
analyze
immediately.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to 4°C and adjust to pH <
2 with H2SO4, HCI, or solid
NaHSO4. Store samples in
capped vials, with no
headspace in an area free of
solvent fumes. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. <9)
Cool to 4°C. Add sodium sulfite
to remove residual chlorine from
treated water samples and then
adicify to pH < 2 with 6N HCI.(9)
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation. (12)
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean
using a damp, then dry cloth. Seal
the vial with non-reactive tape or
film. Wrap glass vials with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
liquid, n.o.s, 6.1,
Poison, UN3278
Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable liquid,
UN1280. See
Footnote (10) when
bisulfate is used.
Shipping label is
compound
dependent. May
require further
analysis.
Standard carrier
shipping label AND
Sodium arsenite,
aqueous solutions,
6.1, Poison, UN1686
Ch. 4 / 3535A /
832 1B
(EPA SW-846)
Ch. 4 / 5030C /
8260C
(EPA SW-846)
525.2
(EPA OW)
200.7/200.8
(EPA OW)
Sample Collection Information Document
A3-14
June 2010
-------
Drinking Water Samples
Analyte
Sodium azide (analyze as
azide ion)
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl pyrophosphate151
Tetramethylene-
disulfotetramine151
Thallium sulfate (analyze for
total thallium)
Thiodiglycol (TDG)
(degradation product of
HD)<51
Sample
Volume'11
40 ml (one
VOA vial)
1 -4L
Sample Container
40-mL VOA vial
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
28 days
Extract samples
within 7 days
and analyze
extracts within
40 days of
extraction.
Sample Preservation or
Preparation
None
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
Standard carrier
shipping label AND
Strychnine, 6.1,
Poison, UN1692
Source/SAM
Method'31
Adapted from J. of
Forensic Sciences,
43(1): 200-202 /
300.1, Rev 1.0
(EPA OW)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 -4L
1 -4L
120-480
ml
1 -4L
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Plastic or glass (special cleaning
needed)01'
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Samples should
be preserved
with acid within 2
weeks of
collection, and
can be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Minimize
transport and
storage time; if
feasible, analyze
or extract
immediately
upon receipt at
the laboratory.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation. <12)
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples. (9)
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated
carbon)
Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018
Standard carrier
shipping label AND
Pesticides, liquid,
toxic, n.o.s, 6.1,
Poison, UN2902
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
liquid, n.o.s, UN3334
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3520C /
3535A / 8270D
(EPA SW-846)
200.7 / 200.8
(EPA OW)
Ch. 4 / 3535A /
832 1B
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A3-15
June 2010
-------
Drinking Water Samples
Analyte
Thiofanox
1,4-Thioxane
(degradation product of
HDf1
Titanium tetrachloride
(analyze for total titanium)
Triethanolamine (TEA)
(degradation product of HN-
3)(5)
Trimethyl phosphite151
1,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide
(analyze for total vanadium)
Sample
Volume'11
1 -4L
1 -4L
Sample Container
Amber 40- or 60-mL glass bottles
with PTFE-lined screw caps.
Preservative should be added to
bottle before sample collection.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Sample Preservation or
Preparation
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from wastewater
samples.'9'
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Packaging Requirements
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal
the bottle with non-reactive tape or
film. Wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Carbamate
pesticides, liquid,
toxic, 6.1, Poison,
UN2992
Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993
Source/SAM
Method'31
531.2
(EPA OW)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4L
1 -4L
1 -4L
1 -4L
120-480
ml
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Plastic or glass (special cleaning
needed)'11'
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Extract samples
within 7 days of
collection;
analyze within
40 days of
extraction.
Samples should
be preserved
with acid within 2
weeks of
collection, and
can be held 6
months with
acidification. If
not acid
preserved,
analyze
immediately.
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Cool to < 6°C. Add sodium
thiosulfate to remove residual
chlorine from treated drinking
water samples.'9'
Cool to < 6°C and store in the
dark. Add sodium thiosulfate to
remove residual chlorine from
treated drinking water
samples.'9'
Cool to < 6°C and store in the
dark. Add sodium thiosulfate to
remove residual chlorine from
treated drinking water
samples.'9'
Acidify to pH < 2 with nitric acid
and store at 4°C. If arsenic
species are to be determined,
remove unpreserved aliquot
prior to sample preservation.'12'
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
3520C / 3535A /
832 1B
(EPA SW-846)
Ch. 4 / 3535A /
8270D
(EPA SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
Ch. 4 / 3535A /
8330B
(EPA SW-846)
200.7 / 200.8
(EPA OW)
Sample Collection Information Document
A3-16
June 2010
-------
Drinking Water Samples
Analyte
White phosphorus
Sample
Volume'11
0.5 -2 L
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined septum
or lid. Fill to overflowing to
eliminate headspace.
Holding Time
Extracts with
isooctane should
be analyzed
within 30 days.
Sample Preservation or
Preparation
Cool to 4°C and store in the
dark.
Packaging Requirements
Wipe outside of each container
clean using a damp, then dry cloth.
Seal the container with non-reactive
tape or film. Wrap glass containers
with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Source/SAM
Method'31
7580
(EPA SW-846)
Sample Collection Information Document
A3-17
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)(s|
Aldicarb sulfone
Aldicarb sulfoxide
Allyl alcohol
4-Aminopyridine
Ammonia
Ammonium metavanadate
(analyze as total vanadium)
Sample
Size111
TBD
20 - 80 g
20 - 80 g
20 - 80 g
20 - 80 g
20 - 80 g
1-4g
10-40g
Sample Container
250-mL PTFE centrifuge tube
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
VOA vial (with septum) and
magnetic stirring bar
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Analyze immediately
or store at negative (-)
18°C.
Extract samples within
14 days of collection;
analyze within 40
days of extraction.
Extract samples within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
14 days
Extract samples within
7 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH 4-5with0.1N
chloroacetic acid
immediately after collection.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH4-5with0.1N
chloroacetic acid
immediately after collection.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH 4-5with0.1N
chloroacetic acid
immediately after collection.
Store with no headspace.
Cool to 4°C or freeze at <
negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Cool to < 6°C. Add sodium
thiosulfate to remove
residual chlorine from
treated drinking water
samples.'9'
Packaging Requirements
TBD
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each bottle clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in
Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Acrylamide, 6.1,
Poison, UN3426
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid, 6.1,
Poison, UN1093
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Standard carrier
shipping label AND
Allyl alcohol, 3,
Flammable liquid, 6.1,
Poison, UN1098.
Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671
Source/SAM
Method'31
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
Ch. 4/8316
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
831 8A
(EPA SW-846)
831 8A
(EPA SW-846)
831 8A
(EPA SW-846)
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Not of concern in this sample type
200 g
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). <11)
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
None
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Sample Collection Information Document
A4-1
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Arsine
Asbestos
Boron trifluoride
Brodifacoum
Bromadiolone151
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)151
Carfentanil
Carbon disulfide
Sample
Size111
200 g
200 g
Sample Container
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). <11)
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). (11)
Holding Time
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Sample Preservation or
Preparation
None
None
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561
Source/SAM
Method'31
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
50 - 200 g
50 - 200 g
200 g
20 - 80 g
20 - 80 g
1 -4g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). (11)
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
VOA vial (with septum) and
magnetic stirring bar
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
14 days
Cool to < 6°C.
Cool to < 6°C.
None
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH 4-5with0.1N
chloroacetic acid
immediately after collection.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH4-5with0.1N
chloroacetic acid
immediately after collection.
Cool to 4°C or freeze at
< negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Standard carrier
shipping label
Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid,
UN1131
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
831 8A
(EPA SW-846)
831 8A
(EPA SW-846)
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Sample Collection Information Document
A4-2
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Chlorfenvinphos
Chlorine
2-Chloroethanol
3-Chloro-1,2-propanediol|51
Chloropicrin151
Chlorpyrifos
Chlorpyrifos oxon
Crimidine151
Cyanide, Amenable to
chlorination
Cyanide, Total
Sample
Size111
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
1-4g
1-4g
1-4g
1-4g
1-4g
1-4g
VOA vial (with septum) and
magnetic stirring bar
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
14 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Store with no headspace.
Cool to 4°C or freeze at
< negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3580A /
8270D
(EPA SW-846) /
Ch. 4 / 3580A /
8270D
(EPA SW-846) /
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Sample Collection Information Document
A4-3
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Cyanogen chloride'5'
1,2-Dichloroethane
(degradation product of HD)
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl
methylphosphonate (DIMP)
(degradation product of
GB)(5>
Dimethylphosphite151
Sample
Size111
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
Sample Container
VOA vial (with septum) and
magnetic stirring bar
VOA vial (with septum) and
magnetic stirring bar
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
14 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Sample Preservation or
Preparation
Store with no headspace.
Cool to 4°C or freeze at
< negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Store with no headspace.
Cool to 4°C or freeze at
< negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
(Air-tight container containing activated
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Shipping Label121
Standard carrier
shipping label AND
Cyanogen chloride,
2.3, Poisonous gas,
UN1589
Standard carrier
shipping label AND
Ethylene dichloride, 6,
Poison, UN1184
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Source/SAM
Method'31
Ch. 4 / 3585 /
8260C
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
801 5C
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A4-4
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Dimethylphosphoramidic
acid (degradation product
GA)(5)
Diphacinone
Disulfoton
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of
HDf1
EA2192
[Diisopropylaminoethyl
methyl thiolophosphonate]
(hydrolysis product of VX)(S|
Ethylmethyl phosphonate
(EMPA)
(degradation product of
VX)(5>
Sample
Size111
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Cool to < 6°C.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
TBD(7)
Air-tight container containing activated
carbon
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
TBD(7)
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Source/SAM
Method'31
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Sample Collection Information Document
A4-5
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Ethyldichloroarsine (ED)(S|
N-Ethyldiethanolamine
(EDEA) (degradation product
of HN-1)(S|
Ethylene oxide
Fenamiphos151
Fentanyl
Fluoride
Fluoroacetamide
Fluoroacetic acid and
fluoroacetate salts (analyze
for fluoroacetate ion)151
2-Fluoroethanol
Formaldehyde
Sample
Size111
1 -4g
1 -4g
1-4g
1-4g
1-4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
VOA vial (with septum) and
magnetic stirring bar
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
14 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Cool to 4°C or freeze at
< negative (-) 7°C. (Frozen
vials should be placed on
their side.)
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Shipping Label121
Standard carrier
shipping label AND
Ethyldichloro-arsine,
6.1, Poison, UN1892
Standard carrier
shipping label
Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to 40
CFR 172.101 for more
information).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Source/SAM
Method'31
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846) /
Not of concern in this sample type
TBD
1 g per
analysis
1-4g
TBD
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
VOA vial (with septum) and
magnetic stirring bar
TBD
28 days
14 days
TBD
Cool to 4°C.
Store with no headspace.
Cool to 4°C or freeze at
< negative -7°C. (Frozen
vials should be placed on
their side.)
TBD
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label
Adapted from
Journal of
Chromatography
6,876(1): 103-
Adapted from
Analytical Letters,
27(14): 2703-2718
/ 300.1, Rev 1.0
(EPA OW)
3585 / 8260C
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A4-6
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Gasoline Range Organics
Hexahydro-1,3,5-trinitro-1,3,5
triazine (RDX)
Hexamethylenetriperoxide-
diamine (HMTD)'51
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide
Hydrogen fluoride
Hydrogen sulfide
Isopropyl methylphosphonic
acid (IMPA) (degradation
product of GB)'51
Kerosene151
Lead arsenate
(analyze for total arsenic)
Lewisite oxide
(degradation product of
Lewisite)'51
Sample
Size111
1 -4g
10-40g
10-40g
Sample Container
VOA vial (with septum) and
magnetic stirring bar
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
14 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool to 4°C or freeze at
< negative -7°C. (Frozen
vials should be placed on
their side.)
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
Packaging Requirements
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier AND
Gasoline identification
set, 2.3, Poison gas,
NA9035
Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483
Forbidden
Source/SAM
Method'31
Ch. 4 / 3585 /
801 5C
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
1-4g
1-4g
200 g
200 g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). (11)
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). <11)
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Cool to < 6°C.
Cool to < 6°C.
None
None
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (2)
when adding liquid
preservatives such as
methanol or bisulfate
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (2)
when adding liquid
preservatives such as
methanol or bisulfate
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
Standard carrier
shipping label AND
Arsenic compounds,
solid, n.o.s, 6.1,
Poison, UN1557
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3585 /
801 5C
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A4-7
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Mercuric chloride (analyze
for total mercury)
Mercury, Total
Methamidophos
Methomyl'51
Methoxyethylmercuric
acetate (analyze for total
mercury)
Methyl acrylonitrile
Methyl fluoroacetate
(analyze for fluoroacetate
ion)'5'
Methyl hydrazine151
Methyl isocyanate
Methyl paraoxon
Methyl parathion
Methylamine
Sample
Size111
Sample Container
Holding Time
Sample Preservation or
Preparation
Packaging Requirements
Shipping Label121
Source/SAM
Method'31
Not of concern in this sample type
Not of concern in this sample type
15g
20 - 80 g
250-mL PTFE centrifuge tube
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
Analyze immediately
or store at negative (-)
18°C.
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
TBD
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH4-5with0.1N
chloroacetic acid
immediately after collection.
TBD
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
831 8A
(EPA SW-846)
Not of concern in this sample type
20 - 80 g
1 g per
analysis
1-4g
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples within
14 days of collection;
analyze within 40
days of extraction
28 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Cool to < 6°C.
Cool to 4°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
Standard carrier
shipping label AND
Methyl hydrazine, 6.1,
Poison, UN1244
Ch. 4/8316
(EPA SW-846)
Adapted from
Analytical Letters,
27(14): 2703-2718
/ 300.1, Rev 1.0
(EPA OW)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4g
1 -4g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A4-8
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
N-Methyldiethanolamine
(MDEA) (degradation
product of HN-2)(S|
Methylphosphonic acid
(MPA)
(degradation product of VX,
GB, & GD)(S|
Mevinphos
Monocrotophos
Nicotine compounds
(analyze as nicotine)
Octahydro-1,3,5,7-tetranitro-
1,3,5,7-tetrazocine(HMX)
O rganophosphate
Pesticides, NOS
Osmium tetraoxide (analyze
for total osmium)
Sample
Size111
1 -4g
1 -4g
1 -4g
1 -4g
1 -4g
10-40g
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Nicotine compounds,
solid, n.o.s, 6.1,
Poison, UN1655
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A4-9
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Oxarnyl'51
Paraquat
Paraoxon
Parathion
Pentaerythritol tetranitrate
(PETN)
Phencyclidine
Phenol
Phorate
Sample
Size111
20 - 80 g
Sample Container
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
Holding Time
Extract sample within
7 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Store at 4 (±2)°C and out of
direct sunlight. Acidify to
pH 4-5with0.1N
chloroacetic acid
immediately after collection.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Source/SAM
Method'31
831 8A
(EPA SW-846)
Not of concern in this sample type
1 -4g
1 -4g
10-40g
1 -4g
1 -4g
1 -4g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Forbidden
Standard carrier
shipping label
Standard carrier
shipping label AND
Phenol, solid, 6.1,
Poison, UN1671
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Sample Collection Information Document
A4-10
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Phorate sulfone
Phorate sulfoxide
Phosgene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl phosphonic
acid (PMPA) (degradation
product of GD)'S|
Propylene oxide'5'
Semivolatile Organic
Compounds, NOS
Sodium arsenite (analyze for
total arsenic)151
Sample
Size111
1 -4g
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
1-4g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1-4g
1-4g
1-4g
200 g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
VOA vial (with septum) and
magnetic stirring bar
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). <11)
Extract samples within
14 days of collection;
analyze within 40
days of extraction.
14 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Cool to < 6°C.
Cool to 4°C or freeze at <
negative (-) 7°C. (Frozen
vials should be set on their
side.)
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
None
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial
with non-reactive tape or film. Wrap
glass vials with bubble wrap. Pack
samples as described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable, UN1280
Shipping label is
compound dependent.
May require further
analysis.
Standard carrier
shipping label AND
Sodium arsenite,
solid, 6.1, Poison,
UN2027
Ch. 4 / 3580A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3585 /
8260C
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Sample Collection Information Document
A4-11
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
Sodium azide (analyze as
azide ion)
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl pyrophosphate'5'
Tetramethylene-
disulfotetramine151
Thallium sulfate (analyze for
total thallium)
Thiodiglycol (TDG)
(degradation product of
HD)(5>
Thiofanox
Sample
Size111
1 g per
analysis
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
28 days
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool to 4°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
Standard carrier
shipping label AND
Strychnine, 6.1,
Poison, UN1692
Source/SAM
Method'31
Ch. 4 / 3580A
(SW-846)/ 300.1,
Rev 1.0 (EPA
OW)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 -4g
1 -4g
200 g
1-4g
1-4g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). (11)
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
None
Cool to < 6°C.
Cool to < 6°C.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Pesticides, solid,
toxic, n.o.s, 6.1,
Poison, UN2588
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
solid, n.o.s, UN3335
Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Ch. 4 / 3580A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Sample Collection Information Document
A4-12
June 2010
-------
Non-aqueous Liquid/Organic Solid Samples'14'
Analyte
1,4-Thioxane
(degradation product of
HD)(5>
Titanium tetrachloride
(analyze for total Titanium)
Triethanolamine (TEA)
(degradation product of HN-
3)(5>
Trimethyl phosphite151
1,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide
(analyze for total vanadium)
White phosphorus
Sample
Size111
1 -4g
Sample Container
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993
Source/SAM
Method'31
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Not of concern in this sample type
1 -4g
1 -4g
10-40g
10-40g
200 g
40- 160 g
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special
cleaning needed). <11)
Wide-mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-
lined septa or lids are not
available, solvent rinsed
aluminum foil may be used as a
liner.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Digest samples as
soon as possible after
arrival; analyze within
180 days of digestion.
Can be held
indefinitely in the dark
if sealed tightly to
eliminate moisture
loss. Extracts with
isooctane should be
analyzed within 30
days.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
Store samples and extracts
at < 4°C in the dark. After
air drying, soil and solid
samples can be stored at
room temperature.
None
Cool to 4°C and store in the
dark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or
film. Wrap glass containers with
bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381
Ch. 4 / 3580A /
832 1B
(EPA SW-846)
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
7580
(EPA SW-846)
Sample Collection Information Document
A4-13
June 2010
-------
Solid Samples
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)
Aldicarb sulfone
Aldicarb sulfoxide
Allyl alcohol
Sample
Size111
TBD
20 - 80 g
20 - 80 g
20 - 80 g
20 - 80 g
20 - 80 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
Sample Container
250-mL PTFE
centrifuge tube
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
Clear, wide-mouth
glass jar with PTFE-
lined lid
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
40-mL VOA vial (with
septum) and
magnetic stirring bar
Holding Time
Analyze immediately
or store at negative
(-)18°C.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
14 days
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Store at 4 (±2)°C and out of
direct sunlight.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to pH 4
- 5 with 0.1N chloroacetic acid
immediately after collection.
Store at 4 (±2)°C and out of
direct sunlight. Acidify to pH 4
- 5 with 0.1N chloroacetic acid
immediately after collection.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Packaging Requirements
TBD
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Acrylamide, solid,
6.1, Poison, UN2074
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid,
6.1, Poison, UN1093
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Allyl alcohol, 3,
Flammable liquid,
6.1, Poison, UN1098.
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Source/SAM
Method'31
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
Ch. 4/8316
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
Ch. 4/831 8A
(EPA SW-846)
Ch. 4/831 8A
(EPA SW-846)
Ch. 4/831 8A
(EPA SW-846)
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Sample Collection Information Document
A5-1
June 2010
-------
Solid Samples
Analyte
4-Aminopyridine
Ammonia
Ammonium metavanadate
(analyze as total vanadium)
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Arsine'51
Asbestos
Boron trifluoride
Sample
Size111
10-40g
Sample Container
Amber glass or PTFE
container with PTFE-
lined septum or lid.
When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Packaging Requirements
Wipe outside of each bottle clean using a
damp, then dry cloth. Seal the bottle with
non-reactive tape or film. Wrap glass
bottles with bubble wrap. Pack samples
as described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671
Source/SAM
Method'31
Ch. 4 / 8330B
(EPA SW-846)
Not of concern in this sample type
200 g
200 g
200 g
1 - 8 g (wet
weight) or 1 -
4g (dry
weight)
200 ml for
soft surfaces;
wipe for hard
surfaces
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).(11)
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
Plastic or glass
(special cleaning
needed)'11'
Wipe (hard surfaces);
25- or 37-mm air
sampling cassette
transferred to
aqueous suspension
of 200 ml (soft
surfaces)
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
None
None
None
None
None
None
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561
Standard carrier
shipping label AND
Arsine, 2.3(2.1),
Poisonous gas,
UN2188
Standard carrier
shipping label
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
Ch. 3 / 3050B /
7010
(EPA SW-846)
D5755-03 (soft
surfaces-
microvac)
(ASTM)
Not of concern in this sample type
Sample Collection Information Document
A5-2
June 2010
-------
Solid Samples
Analyte
Brodifacoum
Bromadiolone15'
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)
Carfentanil
Carbon disulfide
Sample
Size111
50 - 200 g
50 - 200 g
200 g
20 - 80 g
50 - 200 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
Sample Container
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).(11)
Clear, wide mouth
glass jar with PTFE-
lined lid
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Extract within 7 days
of collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
14 days
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
None
Store at 4 (±2)°C and out of
direct sunlight.
Cool to < 6°C.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label
Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid,
UN1131.See
Footnote (11) when
methanol or bisulfate
is used.
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846)
831 8A
(EPA SW-846)
Ch, 4 / 3541 /
3545A / 8321 B
(EPA SW-846)
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Sample Collection Information Document
A5-3
June 2010
-------
Solid Samples
Analyte
Chlorfenvinphos
Chlorine
2-Chloroethanol
3-Chloro-1,2-propanediol|51
Chloropicrin151
Chlorpyrifos
Sample
Size111
1-4g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Not of concern in this sample type
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
30- 120 g
30- 120 g
30- 120 g
40-mL VOA vial (with
septum) and
magnetic stirring bar
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
14 days
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label. See
Footnote (2) when
adding liquid
preservatives such
as methanol or
bisulfate
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Sample Collection Information Document
A5-4
June 2010
-------
Solid Samples
Analyte
Chlorpyrifos oxon
Crimidine151
Cyanide, Amenable to
chlorination
Cyanide, Total
Cyanogen chloride151
1,2-Dichloroethane
(degradation product of HD)
Sample
Size111
30- 120 g
30- 120 g
30- 120 g
1-5g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Glass or polyethylene
bottle (1-L size)
Glass or polyethylene
bottles
40-mL VOA vial (with
septum) and
magnetic stirring bar
40-mL VOA vial (with
septum) and
magnetic stirring bar
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
14 days
12 days after sample
receipt
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
14 days
Sample Preservation or
Preparation
Cool to < 6°C.
Cool to < 6°C.
Add NaOH until pH > 12 at
time of collection; cool to 4°C.
Protect from light and ice or
refrigerate at 4 (±2)°C from
collection until digestion.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Preserve to pH £ 2 with
NaHSO4 (low analyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4). (Air-tight
container containing activated carbon)
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Standard carrier
shipping label AND
Cyanide inorganic
solid n.o.s, 6.1,
Poison, UN1588
Standard carrier
shipping label AND
Cyanides, inorganic,
solid, n.o.s, 6.1,
Poison, UN1588
Standard carrier
shipping label AND
Cyanogen chloride,
2.3, Poisonous gas,
UN1589. See
Footnote (11) when
methanol or bisulfate
is used.
Standard carrier
shipping label AND
Ethylene dichloride,
6, Poison, UN1184.
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
3135.21
(EPA RLAB)
ILM05.3CN
(EPA CLP)
Ch. 4 / 5035A /
8260C
(EPA SW-846) /
Footnote (8)
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Sample Collection Information Document
A5-5
June 2010
-------
Solid Samples
Analyte
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl methylphosphonate
(DIMP)
(degradation product of GB)|S|
Dimethylphosphite151
Dimethylphosphoramidic acid
(degradation product GA)|S|
Sample
Size111
30- 120 g
30- 120 g
30- 120 g
30- 120 g
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Cool to 4°C.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Source/SAM
Method'31
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3541 /
3545A/8015C
(EPA SW-846)
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Sample Collection Information Document
A5-6
June 2010
-------
Solid Samples
Analyte
Diphacinone
Disulfoton
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of HD)|S|
EA2192 [Diisopropylaminoethyl
methyl thiolophosphonate]
(hydrolysis product of VX)(S|
Ethylmethyl phosphonate
(EMPA)
(degradation product of VX)(S|
Sample
Size111
30- 120 g
30- 120 g
30- 120 g
30- 120 g
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Sample Preservation or
Preparation
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Cool to < 6°C.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
TBD(7)
Air-tight container containing activated
carbon
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label. See
Footnote (2) when
adding liquid
preservatives such
as methanol or
bisulfate
TBD(7)
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Source/SAM
Method'31
3541 / 3545A /
832 1B
(EPA SW-846)
3541 / 3545A /
8270D
(EPA SW-846)
3541 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
832 1B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Sample Collection Information Document
A5-7
June 2010
-------
Solid Samples
Analyte
Ethyldichloroarsine (ED)(S|
N-Ethyldiethanolamine (EDEA)
(degradation product of HN-1)(S|
Ethylene oxide
Fenamiphos151
Fentanyl
Fluoride
Fluoroacetamide
Sample
Size111
30- 120 g
30- 120 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
14 days
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool to < 6°C.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
-7°C. (Frozen vials should be
placed on their side.)
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Cool to < 6°C.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Ethyldichloroarsine,
6.1, Poison, UN1892
Standard carrier
shipping label
Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to
40CFR 172.101 for
more information).
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Source/SAM
Method'31
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846) /
Footnote (8)
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846) /
Not of concern in this sample type
TBD
TBD
TBD
TBD
TBD
Standard carrier
shipping label
Adapted from
Journal of
Chromatography
6,876(1): 103-
Sample Collection Information Document
A5-8
June 2010
-------
Solid Samples
Analyte
Fluoroacetic acid and
fluoroacetate salts (analyze for
fluoroacetate ion)151
2-Fluoroethanol
Formaldehyde
Gasoline Range Organics
Hexahydro-1,3,5-trinitro-1,3,5-
triazine (RDX)
Sample
Size111
30- 120 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
25- 100 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
10-40g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Analyze samples
within 28 days.
14 days
Extract and analyze
within 30 days of
collection. All
derivatized sample
extracts should be
analyzed within 3
days after
preparation.
14 days
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
None
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-)
7°C. (Frozen vials should be
placed on their side.)
Refrigerate at 4°C (solid
extracts should be buffered to
pH 5.0 to minimize the
formation of artifact
formaldehyde).
Preserve to pH £ 2 with
NaHSO4 (low analyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-)
7°C. (Frozen vials should be
placed on their side.)
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to
40CFR 172.101 for
more information).
Standard carrier AND
Gasoline
identification set, 2.3,
Poison gas, NA9035.
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483
Source/SAM
Method'31
Adapted from
Analytical Letters,
27(14): 2703-2718
/ 300.1, Rev 1.0
(EPA OW)
5035A / 8260C
(EPA SW-846)
831 5A
(EPA SW-846)
Ch. 4 / 5035A /
801 5C
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Sample Collection Information Document
A5-9
June 2010
-------
Solid Samples
Analyte
Hexamethylenetriperoxide-
diamine (HMTD)'51
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide
Hydrogen fluoride
Hydrogen sulfide
Isopropyl methylphosphonic acid
(IMPA) (degradation product of
GB)<51
Kerosene151
Lead arsenate
(analyze for total arsenic)
Lewisite oxide
(degradation product of
Lewisite)'51
Sample
Size111
10-40g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label'21
Forbidden
Source/SAM
Method'31
Ch. 4 / 8330B
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
30- 120 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
200 g
200 g
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
14 days
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Cool to < 6°C.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
None
None
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
Standard carrier
shipping label AND
Arsenic compounds,
solid, n.o.s, 6.1,
Poison, UN1557
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Ch. 4 / 5035A /
801 5C
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846) /
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846) /
Footnote (8)
Sample Collection Information Document
A5-10
June 2010
-------
Solid Samples
Analyte
Mercuric chloride (analyze for
total mercury)
Mercury, Total
Methamidophos
Methomyl
Methoxyethylmercuric acetate
(analyze for total mercury)151
Methyl acrylonitrile
Methyl fluoroacetate (analyze for
fluoroacetate ion) |s|
Methyl hydrazine151
Methyl isocyanate
Sample
Size111
200 g
200 g
15g
20 - 80 g
200 g
20 - 80 g
30- 120 g
30- 120 g
Sample Container
PTFE, plastic or glass
PTFE, plastic or glass
250-mL PTFE
centrifuge tube
Clear, wide mouth
glass jar with PTFE-
lined lid
PTFE, plastic or glass
250-mL wide-mouth
glass container with
PTFE-lined septa or
lid
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
28 days
28 days
Analyze immediately
or store at negative
(-)18°C.
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
28 days
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction
Analyze samples
within 28 days.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Acidify to pH < 2 with nitric
acid.
Acidify to pH < 2 with nitric
acid.
TBD
Store at 4 (±2)°C and out of
direct sunlight.
Acidify to pH < 2 with nitric
acid.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
TBD
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
bottle with non-reactive tape or film.
Wrap glass bottles with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Mercuric chloride,
6.1, Poison, UN1624
Standard carrier
shipping label AND
Mercury, 8,
Corrosive, UN2809
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Mercury compounds,
solid, n.o.s, 6.1,
Poison, UN2025
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
Standard carrier
shipping label AND
Methyl hydrazine,
6.1, Poison, UN1244
Source/SAM
Method'31
Ch. 3 / 7473
(EPA SW-846)
Ch. 3 / 7473
(EPA SW-846)
Adapted from
Journal of
Chromatography
A, 11 54(1): 3-25
831 8A
(EPA SW-846)
Ch. 3 / 7473
(EPA SW-846)
Ch. 4/8316
(EPA SW-846)
Adapted from
Analyical Letters,
27(14): 2703-2718
/ 300.1, Rev 1.0
(EPA OW)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A5-11
June 2010
-------
Solid Samples
Analyte
Methyl paraoxon
Methyl parathion
Methylamine
N-Methyldiethanolamine (MDEA)
(degradation product of HN-2)(S|
Methylphosphonic acid (MPA)
(degradation product of VX, GB,
& GD)(S|
Mevinphos
Sample
Size111
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract sample within
14 days of collection;
analyze within 40
days of extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3580A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Not of concern in this sample type
30- 120 g
30- 120 g
30- 120 g
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846)
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Sample Collection Information Document
A5-12
June 2010
-------
Solid Samples
Analyte
Monocrotophos
Nicotine compounds (analyze as
nicotine)
Octahydro-1,3,5,7-tetranitro-
1,3,5,7-tetrazocine(HMX)
Organophosphate pesticides,
NOS
Osmium tetraoxide (analyze for
total osmium)151
Oxamyl
Paraquat
Sample
Size111
30- 120 g
30- 120 g
10-40g
30- 120 g
200 g
20 - 80 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).01'
Clear, wide mouth
glass jar with PTFE-
lined lid.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Extract samples
within 7 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
None
Store at 4 (±2)°C and out of
direct sunlight.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Nicotine compounds,
solid, n.o.s, 6.1,
Poison, UN1655
Standard carrier
shipping label
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid,
n.o.s, 6.1, Poison,
UN2783
Standard carrier
shipping label AND
Osmium tetroxide,
6.1, Poison, UN2471
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Source/SAM
Method'31
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 3 / 3050B /
601 OC
(EPA SW-846)
831 8A
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A5-13
June 2010
-------
Solid Samples
Analyte
Paraoxon
Parathion
Pentaerythritol tetranitrate
(PETN)
Phencyclidine
Phenol
Phorate
Sample
Size111
30- 120 g
30- 120 g
10-40g
30- 120 g
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Forbidden
Standard carrier
shipping label
Standard carrier
shipping label AND
Phenol, solid, 6.1,
Poison, UN1671
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Sample Collection Information Document
A5-14
June 2010
-------
Solid Samples
Analyte
Phorate sulfone
Phorate sulfoxide
Phosgene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl phosphonic
acid (PMPA) (degradation
product of GD)(S|
Propylene oxide151
Sample
Size111
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Source/SAM
Method'31
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Not of concern in this sample type
30- 120 g
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
30- 120 g
5-20g
When
collected
without
preservative,
fill the
container,
leaving no
headspace.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
40-mL VOA vial (with
septum) and
magnetic stirring bar.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Stable for 7 days on
ice and 14 days with
chemical
preservatives.
Analyze as soon as
practical.
Cool to < 6°C.
Preserve to pH £ 2 with
NaHSO4(lowanalyte
concentrations) or methanol
(high analyte concentrations)
and cool to 4°C. Freeze
unpreserved samples at
negative (-) 7°C. (Frozen vials
should be placed on their
side.)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each vial clean using a
damp, then dry cloth. Seal the vial with
non-reactive tape or film. Wrap glass
vials with bubble wrap. Pack samples as
described in Footnote (4).
Standard carrier
shipping label AND
Organophosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable, UN1280.
See Footnote (2)
when adding liquid
preservatives such
as methanol or
bisulfate
Ch. 4 / 3545A /
832 1B
(EPA SW-846)
Ch. 4 / 5035A /
8260C
(EPA SW-846)
Sample Collection Information Document
A5-15
June 2010
-------
Solid Samples
Analyte
Semivolatile organic compounds,
NOS
Sodium arsenite (analyze for
total arsenic)151
Sodium azide (analyze as axide
ion)
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl pyrophosphate151
Sample
Size111
30- 120 g
200 g
30- 120 g
30- 120 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Analyze samples
within 28 days.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
None
Cool to 4°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Shipping label is
compound
dependent. May
require further
analysis.
Standard carrier
shipping label AND
Sodium arsenite,
solid, 6.1, Poison,
UN2027
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
Standard carrier
shipping label AND
Strychnine, 6.1,
Poison, UN1692
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846)
Adapted from J. of
Forensic
Sciences, 43(1):
200-202/300.1,
Rev 1.0 (EPA
OW)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
30- 120 g
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Sample Collection Information Document
A5-16
June 2010
-------
Solid Samples
Analyte
Tetramethylene-
disulfotetramine151
Thallium sulfate (analyze for total
thallium)
Thiodiglycol (TDG)
(degradation product of HD)|S|
Thiofanox
1,4-Thioxane
(degradation product of HD)|S|
Titanium tetrachloride (analyze
for total titanium)151
Sample
Size111
30- 120 g
200 g
30- 120 g
30- 120 g
30- 120 g
200 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).'11'
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).(11)
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time; if
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Sample Preservation or
Preparation
Cool samples to < 6°C and
store extracts at negative (-)
10°Cinthedark.
None
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
None
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Pesticides, solid,
toxic, n.o.s, 6.1,
Poison, UN2588
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
solid, n.o.s, UN3335
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993
Standard carrier
shipping label AND
Titanium
tetrachloride, 8,
Corrosive, UN1838
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8270D
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846)
Ch. 4 / 3545A /
832 1B
(EPA SW-846) /
Footnote (8)
3541 / 3545A /
832 1B
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 3 / 3050B /
6010C/6020A
(EPA SW-846)
Sample Collection Information Document
A5-17
June 2010
-------
Solid Samples
Analyte
Triethanolamine (TEA)
(degradation product of HN-3)(S|
Trimethyl phosphite151
1,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide (analyze for
total vanadium)
White phosphorus
Sample
Size111
30- 120 g
30- 120 g
10-40g
10-40g
200 g
40- 160 g
Sample Container
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
PTFE, plastic or
glass. If using 6020A,
only PTFE or
fluorocarbon
containers are
recommended.
(special cleaning
needed).01'
Wide mouth glass or
PTFE container with
PTFE-lined septum or
lid. When PTFE-lined
septa or lids are not
available, solvent
rinsed aluminum foil
may be used as a
liner.
Holding Time
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Extract samples
within 14 days of
collection; analyze
within 40 days of
extraction.
Digest samples as
soon as possible
after arrival; analyze
within 180 days of
digestion.
Soil / sediment
samples can be held
indefinitely if sealed
tightly to eliminate
moisture loss.
Extracts with
isooctane should be
analyzed within 30
days.
Sample Preservation or
Preparation
Cool to < 6°C.
Cool samples to < 6°C and
store extracts at negative (-)
10°C in the dark.
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
Store samples and extracts at
< 4°C in the dark. After air
drying, soil and solid samples
can be stored at room
temperature.
None
Cool to 4°C and store in the
dark.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Pack samples as described in Footnote
(4).
Shipping Label121
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381
Source/SAM
Method'31
Ch. 4 / 3541 /
3545A / 8321 B
(EPA SW-846)
Ch. 4 / 3545A /
8270D
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 4 / 8330B
(EPA SW-846)
Ch. 3 / 3031 /
6010C/6020A
(EPA SW-846)
7580
(EPA SW-846)
Sample Collection Information Document
A5-18
June 2010
-------
Wipe Samples
Analyte
Acephate
Acrylamide
Acrylonitrile
Aldicarb (Temik)
Aldicarb sulfone
Aldicarb sulfoxide
Allvl alcohol
4-Aminopyridine
Ammonia
Ammonium
metavanadate
(analyze as total
vanadium)
Sample
Size111
TBD (Type
and
number of
wipes
TBD)
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
Sample Container
250-mL PTFE centrifuge tube
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
Clear, wide mouth glass jar with
PTFE-lined lid
Clear, wide mouth glass jar with
PTFE-lined lid
Clear, wide mouth glass jar with
PTFE-lined lid
Holding Time
Analyze immediately or
store at negative (-) 18 C.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Sample Preservation
or Preparation
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Store at 4 (±2)°C and
out of direct sunlight.
Store at 4 (±2)°C and
out of direct sunlight.
Store at 4 (±2)°C and
out of direct sunlight.
Packaging Requirements
TBD
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Shipping Label'2'
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Acrylamide, solid,
6.1, Poison, UN2074
Standard carrier
shipping label AND
Acrylonitrile,
stabilized, 3,
Flammable liquid, 6.1,
Poison, UN1093
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Source/SAM
Method'31
Adapted from Journal
of Chromatography A,
11 54(1): 3-25
Ch. 4 / 3570 / 8290A
Appendix A/ 831 6
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 831 6
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 831 8A
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 831 8A
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 831 8A
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671
Ch. 4 / 3570 / 8290A
Appendix A / 8330B
(EPA SW-846)
Not of concern in this sample type
TBD
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
None
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859
9102(NIOSH)/Ch. 3
/ 6010C/6020A
(EPA SW-846)
Sample Collection Information Document
A6-1
June 2010
-------
Arsenic, Total
Arsenic trioxide
(analyze for total arsenic)
Arsine'51
Asbestos
Boron trifluoride
Brodifacoum
Bromadiolone'5'
Calcium arsenate
(analyze as total arsenic)
Carbofuran (Furadan)
Carfentanil
Carbon disulfide
TBD
TBD
TBD
1 particle-
free wipe
per 100
cm2
PTFE, plastic or glass. If using
6020A only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
PTFE, plastic or glass. If using
6020A only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
Plastic or glass (special cleaning
needed)'1 1)
Clean, scalable container
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Minimize transport and
storage time; if feasible,
analyze or extract
immediately upon receipt at
the laboratory.
None
None
None
None
None
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558
Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561
Standard carrier
shipping label AND
Arsine, 2.3(2.1),
Poisonous gas,
UN2188
Standard carrier
shipping label
9102(NIOSH)/Ch. 3
/ 6010C/6020A
(EPA SW-846)
9102(NIOSH)/Ch. 3
/ 6010C/6020A
(EPA SW-846)
9102(NIOSH)/7010
(EPA SW-846)
D6480-05 (hard
surfaces-wipes)
(ASTM)
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
TBD
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
PTFE, plastic or glass. If using
6020A only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
Clear, wide mouth glass jar with
PTFE-lined lid
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool to < 6°C.
Cool to < 6°C.
None
Store at 4 (±2)°C and
out of direct sunlight.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Calcium arsenate,
6.1, Poison, UN1573
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A(EPA
SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 831 8A
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Not of concern in this sample type
Sample Collection Information Document
A6-2
June 2010
-------
Chlorfenvinphos
Chlorine
2-Chloroethanol
3-Chloro-1,2-
propanediol'5'
Chloropicrin'5'
Chlorpyrifos
Chlorpyrifos oxon
Crimidine151
Cyanide, Amenable to
chlorination
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
TBD
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Glass or polyethylene bottle (1-L
size)
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
1 4 days
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool to 4°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN1580
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Standard carrier
shipping label AND
Cyanide inorganic
solid n.o.s, 6.1,
Poison, UN1588
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
3135.21
(EPA RLAB)
Sample Collection Information Document
A6-3
June 2010
-------
Cyanide, Total
Cyanogen chloride'5'
1,2-Dichloroethane
Dichlorvos
Dicrotophos
Diesel Range Organics
Diisopropyl
methylphosphonate
(DIMP)
(degradation product of
GB)(5>
Dimethylphosphite151
Dimethylphosphoramidic
acid (degradation
product GA)(S|
TBD
Glass or polyethylene bottles
12 days after sample receipt
Protect from light and
ice or refrigerate at 4
(±2)°C from collection
until digestion.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4). (Air-tight container containing
activated carbon)
Standard carrier
shipping label AND
Cyanides, inorganic,
solid, n.o.s, 6.1,
Poison, UN1588
ILM05.3 CN
(EPA CLP)
Not of concern in this sample type
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Standard carrier
shipping label
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 801 5C
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Sample Collection Information Document
A6-4
June 2010
-------
Diphacinone
Disulfoton
Disulfoton sulfoxide
1,4-Dithiane
(degradation product of
HD)(5>
EA2192
[Diisopropylaminoethyl
methyl
thiolophosphonate]
(hydrolysis product of
VX)(51
Ethylmethyl phosphonate
(EMPA)
(degradation product of
VX)(5>
Ethyldichloroarsine
(ED)(51
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
TBD
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool to < 6°C.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool to < 6°C.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
TBD(7)
Air-tight container containing activated carbon
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid,
toxic, 6.1, Poison,
UN3026
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label. See
Footnote (2) when
adding liquid
preservatives such as
methanol or bisulfate
TBD(7)
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Ethyldichloroarsine,
6.1, Poison, UN1892
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
9102(NIOSH)/Ch. 4
/ 8270D
(EPA SW-846)
Sample Collection Information Document
A6-5
June 2010
-------
N-Ethyldiethanolamine
(EDEA) (degradation
product of HN-1)(S|
Ethylene oxide
Fenamiphos151
Fentanyl
Fluoride
Fluoroacetamide
Fluoroacetic acid and
fluoroacetate salts
(analyze for fluoroacetate
ion)'51
2-Fluoroethanol
Formaldehyde
Gasoline Range Organics
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846) /
Footnote (8)
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label
Ch. 4 / 3570 / 8290A
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Not of concern in this sample type
TBD
1 wiper per
24in2
TBD
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
TBD
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
TBD
Cool to < 6°C.
TBD
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label
Adapted from Journal
of Chromatography B,
876(1): 103-108
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(SW-846)/ 300.1,
Rev 1 .0 (EPA OW)
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
40-mL VOA vial (with septum)
and magnetic stirring bar
Extract and analyze within
30 days of collection. All
derivatized sample extracts
should be analyzed within 3
days after preparation.
14 days
Cool to 4°C.
Cool to 4°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to
40CFR172.101 for
more information).
Standard carrier AND
Gasoline
identification set, 2.3,
Poison gas, NA9035.
See Footnote (2)
when adding liquid
preservatives such as
methanol or bisulfate
Ch. 4 / 3570 / 8290A
Appendix A/ 831 5A
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 801 5C
(EPA SW-846)
Sample Collection Information Document
A6-6
June 2010
-------
Hexahydro-1 ,3,5-trinitro-
1,3,5-triazine(RDX)
Hexamethylenetriperoxid
e-diamine (HMTD)(S|
Hydrogen bromide
Hydrogen chloride
Hydrogen cyanide
Hydrogen fluoride
Hydrogen sulfide
Isopropyl
methylphosphonic acid
(IMPA) (degradation
product of GB)(S|
Kerosene'51
Lead arsenate
(analyze for total arsenic)
Lewisite oxide
(degradation product of
Lewisite)151
Mercuric chloride
(analyze for total
mercury)
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483
Forbidden
Ch. 4 / 3570 / 8290A
Appendix A / 8330B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A / 8330B
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
TBD
TBD
TBD
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
40-mL VOA vial (with septum)
and magnetic stirring bar
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed).(11)
PTFE, plastic or glass
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
14 days
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Minimize transport and
storage time; if feasible,
analyze or extract
immediately upon receipt at
the laboratory.
28 days
Cool to < 6°C.
Cool to 4°C.
None
None
None
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223.
See Footnote (2)
when adding liquid
preservatives such as
methanol or bisulfate
Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617
Standard carrier
shipping label AND
Arsenic compounds,
solid, n.o.s, 6.1,
Poison, UN1557
Standard carrier
shipping label AND
Mercuric chloride,
6.1, Poison, UN1624
Ch. 4 / 3570 / 8290A
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290A
Appendix A/ 801 5C
(EPA SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A(EPA
SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A(EPA
SW-846)
9102(NIOSH)/7473
(EPA SW-846)
Sample Collection Information Document
A6-7
June 2010
-------
Mercury, Total
Methamidophos
Methomyl
M eth oxyeth y 1 m ercu ri c
acetate (analyze for total
mercury)151
Methyl acrylonitrile
Methyl fluoroacetate
(analyze for fluoroacetate
ion)151
Methyl hydrazine'51
Methyl isocvanate
Methyl paraoxon
TBD
TBD
1 wiper per
24in2
TBD
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
PTFE, plastic or glass
250-mL PTFE centrifuge tube
Clear, wide mouth glass jar with
PTFE-lined lid
PTFE, plastic or glass
250-mL wide-mouth glass
container with PTFE-lined septa
or lid
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
28 days
Analyze immediately or
store at negative (-) 18°C
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
28 days
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Analyze samples within 28
days.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
None
TBD
Store at 4 (±2)°C and
out of direct sunlight.
None
Cool to < 6°C.
None
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
TBD
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Mercury, 8,
Corrosive, UN2809
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Standard carrier
shipping label AND
Mercury compounds,
solid, n.o.s, 6.1,
Poison, UN2025
Standard carrier
shipping label AND
Methyl acryonitrile,
3.0, Flammable
Liquid, 6.1, Poison,
UN3079
Standard carrier
shipping label
Standard carrier
shipping label AND
Methyl hydrazine, 6.1,
Poison, UN1244
9102(NIOSH)/7473
(EPA SW-846)
Adapted from Journal
of Chromatography A,
11 54(1): 3-25
Ch. 4 / 3570 / 8290
Appendix A/ 831 8A
(EPA SW-846)
9102(NIOSH)/7473
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A/ 831 6
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A (EPA SW-
846) / 300.1, Rev 1.0
(EPA OW)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Sample Collection Information Document
A6-8
June 2010
-------
Methyl parathion
Methylamine
N-Methyldiethanolamine
(MDEA) (degradation
product of HN-2)(S|
Methylphosphonic acid
(MPA)
(degradation product of
VX, GB, & GD)(S|
Mevinphos
Monocrotophos
Nicotine compounds
(analyze as nicotine)
Octahydro-1, 3,5,7-
tetranitro-1, 3,5,7-
tetrazocine (HMX)
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool to < 6°C.
Cool to < 6°C.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Nicotine compounds,
solid, n.o.s, 6.1,
Poison, UN1655
Standard carrier
shipping label
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8330B
(EPA SW-846)
Sample Collection Information Document
A6-9
June 2010
-------
Organophosphate
pesticides, NOS
Osmium tetraoxide
(analyze for total
osmium)151
Oxamyl
Paraquat
Paraoxon
Parathion
Pentaerythritol
tetranitrate (PETN)
Phencyclidine
1 wiper per
24in2
TBD
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed).(11)
Clear, wide mouth glass jar with
PTFE-lined lid
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
None
Store at 4 (±2)°C and
out of direct sunlight.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Osmium tetroxide,
6.1, Poison, UN2471
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
9102(NIOSH)/Ch. 3
/ 601 OC (EPA SW-
846)
Ch. 4 / 3570 / 8290
Appendix A/ 831 8A
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Forbidden
Standard carrier
shipping label
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8330B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Sample Collection Information Document
A6-10
June 2010
-------
Phenol
Phorate
Phorate sulfone
Phorate sulfoxide
Phosgene
Phosphamidon
Phosphine
Phosphorus trichloride
Pinacolyl methyl
phosphonic acid (PMPA)
(degradation product of
GD)(5>
Propylene oxide'5'
Semivolatile organic
compounds, NOS
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Phenol, solid, 6.1,
Poison, UN1671
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Orga no phosphorus
compound, toxic,
solid, n.o.s, 6.1,
Poison, UN3464
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Not of concern in this sample type
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Shipping label is
compound
dependent. May
require further
analysis.
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Sample Collection Information Document
A6-11
June 2010
-------
Sodium arsenite (analyze
for total arsenic)151
Sodium azide (analyze as
axide ion)
Strychnine
Sulfur dioxide
Sulfur trioxide
Tetraethyl
pyrophosphate'5'
Tetramethylene-
disulfotetramine'5'
Thallium sulfate (analyze
for total thallium)
Thiodiglycol (TDG)
(degradation product of
HD)(5>
Thiofanox
TBD
TBD
1 wiper per
24in2
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
Filter / Sorbent tube (PVC or
quartz filter with silica coated
sorbent [1 50 mg/75 mg])
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Analyze samples stored at
room temperature within 10
days.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
None
Refrigerate when not
in transit.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
TBD
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Sodium arsenite,
solid, 6.1, Poison,
UN2027
Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN1687
Standard carrier
shipping label AND
Strychnine, 6.1,
Poison, UN1 692
9102(NIOSH)/Ch. 3
/6010C/6020A(EPA
SW-846)
ID-211 (OSHA)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Not of concern in this sample type
Not of concern in this sample type
1 wiper per
24in2
1 wiper per
24in2
TBD
1 wiper per
24in2
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed).'11'
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
None
Cool to < 6°C.
Cool to < 6°C.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4). (Air-tight container containing
activated carbon)
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4). (Air-tight container containing
activated carbon)
Standard carrier
shipping label AND
Orga no phosphorus
pesticides, solid, 6.1,
Poison, UN2783
Standard carrier
shipping label AND
Pesticides, solid,
toxic, n.o.s, 6.1,
Poison, UN2588
Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707
Standard carrier
shipping label AND
Aviation regulated
solid, n.o.s, UN3335
Standard carrier
shipping label AND
Carbamate
pesticides, solid,
toxic, 6.1, UN2757
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Sample Collection Information Document
A6-12
June 2010
-------
1,4-Thioxane
(degradation product of
HD)(5>
Titanium tetrachloride
(analyze for total
titanium)'51
Triethanolamine (TEA)
(degradation product of
HN-3)(S|
Trimethyl phosphite'5'
1 ,3,5-Trinitrobenzene
(1,3,5-TNB)
2,4,6-Trinitrotoluene
(2,4,6-TNT)
Vanadium pentoxide
(analyze for total
vanadium)
1 wiper per
24in2
TBD
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
1 wiper per
24in2
TBD
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed).(11)
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
Amber glass or PTFE container
with PTFE-lined septum or lid.
When PTFE-lined septa or lids
are not available, solvent rinsed
aluminum foil may be used as a
liner.
PTFE, plastic or glass. If using
6020A, only PTFE or
fluorocarbon containers are
recommended, (special cleaning
needed). (11)
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 14
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Extract samples within 7
days of collection; analyze
within 40 days of extraction.
Digest samples as soon as
possible after arrival;
analyze within 180 days of
digestion.
Cool samples to < 6°C
and store extracts at
negative (-) 10°C in
the dark.
None
Cool to < 6°C.
Cool samples to < 6°C
and store extracts at
negative (-) 10°Cin
the dark.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
Store samples and
extracts at < 4°C in
the dark. After air
drying, soil and solid
samples can be stored
at room temperature.
None
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993
Standard carrier
shipping label AND
Titanium
tetrachloride, 8,
Corrosive, UN1838
Standard carrier
shipping label AND
Ethanolamine
solutions, 8,
Corrosive, UN2491
Standard carrier
shipping label AND
Trimethyl phosphite,
3, Flammable,
UN2329
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene
mixtures, 1.1,
Explosive, UN0388
Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A/ 8321 B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8270D
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8330B
(EPA SW-846)
Ch. 4 / 3570 / 8290
Appendix A / 8330B
(EPA SW-846)
9102(NIOSH)/Ch. 3
/6010C/6020A
(EPA SW-846)
Sample Collection Information Document
A6-13
June 2010
-------
White phosphorus
1 wiper per
24in2
Wide mouth glass or PTFE
container with PTFE-lined
septum or lid. When PTFE-lined
septa or lids are not available,
solvent rinsed aluminum foil may
be used as a liner.
Soil / sediment samples can
be held indefinitely if sealed
tightly to eliminate moisture
loss. Extracts with isooctane
should be analyzed within
30 days.
Cool to 4°C and store
in the dark.
Wipe outside of each container clean using a
damp, then dry cloth. Seal the container with non-
reactive tape or film. Wrap glass containers with
bubble wrap. Pack samples as described in
Footnote (4).
Standard carrier
shipping label AND
Phosphorus, white
(dry or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381
Ch. 4 / 3570 / 8290
Appendix A / 7580
(EPA SW-846)
Footnotes:
(1) Sample sizes are based on a range covering the minimum amount needed for analysis of a single sample up to additional sample amounts that may be requested or needed for laboratory quality control analyses (e.g.,
duplicates, matrix spikes) or for measurement of low analyte concentrations. It is also recommended that additional samples be collected in case of the need for reanalysis due to sample spillage or unforseen analytical
difficulties.
(2) Environmental samples are shipped as nonhazardous cargo if they do not exhibit one of the following U.S. DOT hazard classes: explosive, corrosive, flammable, or poisonous. Only a standard shipping carrier's label is
needed in these cases. If any sample is listed under one of these classes, DOT labeling is required. The DOT label must indicate, PSN (49 CFR 172.301), the UN Number (49 CFR 172.301), appropriate hazard class and
descriptor (49 CFR 172.101), and "Limited Quantity" when a quantity exception is used (49 CFR 172.101). When the quantity limit for a given substance is exceeded, the phrase "Limited Quantity" should be excluded
from the label. Note that there are no "exceptions" for substances belonging to DOT Division 2.1 or Division 2.3. Exception quantities include:
• Exception for Class 3, flammable and combustible liquids: Ensure total net quantity of Class 3 substances does not exceed 1 L (0.3 gal) (49 CFR 173.150)
• Exception for Class 6.1, poisonous liquids: Ensure the total net quantity of Class 6.1 liquids does not exceed 4 L (1 gal) of liquid or 5 kg (11 Ibs) of solid (49 CFR 173.153)
• Exception for Class 8, corrosive materials: Ensure total net quantity of Class 8 materials does not exceed 5 L (1.3 gal) of liquid or 5 kg (11 Ibs) of solid (49 CFR 173.154)
• Shipment of samples containing reactive levels (>12%) of energetic material residues using domestic carriers is prohibited. If samples are known or suspected to
contain >12% of energetic material residues, ship and label according to DOT requirements for secondary explosive materials.
(3) SAM methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center (NHSRC), Standardized Analytical Methods for Environmental Restoration
Following Homeland Security Events, Revision 5.0, September, 2009
(4) Pack sample transport containers outside the contaminated area. Samples must be packed in a manner that protects the integrity of the samples and provides temperature conditions required for sample preservation.
Samples should be surrounded by shock- and water-absorbent packing materials or ice (if required for preservation) and shipped in a cooler to ensure sample temperatures do not exceed preservation requirements. If the
target analytes are unknown, samples should be maintained at < 4°C, but above freezing. Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around,
among, and on top of the sample containers.
(5) Currently, no information is available for this analyte in this matrix. Until such time that analyte-specific information is available, collection procedures described for a similar analyte are considered to be appropriate.
(6) This method applies to ambient concentrations of VOCs above 0.5 ppbv and typically requires VOC enrichment by concentrating up to 1 L of sample volume; however, when using current technologies, quantifications of
approximately 100 pptv have been achieved with 0.5-L sample volumes.
(7) DOT regulations currently do not address labeling, packaging, and shipping of chemical warfare agents (CWAs) or this degradation product. Shipment of samples suspected of containing these compounds should be
determined by the overseeing regulatory authority and must be in compliance with U.S. Department of Defense and/or Department of Homeland Security regulations. Under current U.S. Army regulations (AR 50-6, AR
385-61 and DA PAM 385-61), samples are analyzed and decontaminated on site, and are not removed until CWAs are no longer detected.
(8) In addition to the SAM method listed, the "Organisation for the Prohibition of Chemical Weapons, Quality System Documents" was also used as a source of information.
(9) Sodium thiosulfate or sodium sulfite should be added to treated drinking water or wastewater samples containing residual chlorine. For sodium thiosulfate, an amount equivalent to the following is recommended: 3 mL of a
10% sodium thiosulfate solution per gallon of sample (or 0.008%). For sodium sulfite, an amount equivalent to the following is recommended: to 1-L sample, add 40 - 50 mg of solid sodium sulfite with stiring or shaking.
This solution can be added to the sample container prior to collection or after collection and prior to sample preservation.
(10) When samples are preserved with methanol or sodium bisulfate, quantity limitations apply. To avoid samples to be considered DOT hazardous material, ensure each container holds less than a net of 1 L (0.3 gal) of
methanol or aqueous bisulfate. If these volumes are exceeded, the outer container must be marked as: "Methanol, 3, UN1230, Ltd Qty." In addition, a DOT label marked "Flammable Liquid, Class 3" should be affixed to
the outer container. In the case of sodium bisulfate preservative, the outer container also should be marked "Bisulfate, aqueous solution, 8, UN2837, Ltd Qty" and a DOT label marked "Corrosive material, Class 8" is
required.
(11) Use glassware cleaning instructions found in Chapter 3 of SW-846. Rinse with detergent, followed by tap water, 1:1 nitric acid tap water, 1:1 hydrochloric acid:tap water, and reagent water.
(12) If sample collectors are not able to preserve samples in the field (e.g., acid preservatives are not available), the samples may be preserved in the laboratory. If preserved in the laboratory, however, samples must be held
for at least 24 hours prior to analysis.
(13) Prior to use, wash vials and septa with detergent and rinse with tap and distilled water. Allow the vials and septate air dry at room temperature, place in a 105°C oven for 1 hr, then remove and allow to cool in an area
known to be free of organics.
(14) An organic solid sample is a solid that completely dissolves in an organic solvent and leaves no solid residue.
Sample Collection Information Document
A6-14
June 2010
-------
Sample Collection Information Document A6 -15 June 2010
-------
Attachment B
Sample Collection Information for the Radiolchemical
Analytes Listed in SAM
Sample Collection Information Document June 2010
-------
Attachment B: Sample Collection Information for the Radiochemical Analytes Listed in SAM
Air Filters
Analyte
Gross Alpha
Gross Beta
Gamma
Sample Size111
1 filter (limited to
counting
geometry size)
1 filter (limited to
counting
geometry size)
1 filter (limited to
counting
geometry size)
Sample Container'2'
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'2'
Maximum 6 months
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation'2'
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'3'
Vol. 2, pg. 33
(FRMAC)
Vol. 2, pg. 33
(FRMAC)
Ga-01-R
(HASL-300)
Air Filters — Qualitative Determination
Analyte
Americium-241
Californium-252
Curium-244
Plutonium-238
Plutonium-239
Radium-226
Uranium-238
Uranium-234
Uranium-235
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
Sample Size111
1 filter
1 filter
1 filter
Sample Container'2'
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'2'
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
-125 of 59.4 days
Sample Preservative or
Preparation'2'
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'3'
D3084 (ASTM)
Ga-01-R
(HASL-300)
Procedure #9(9)
(ORISE)
Sample Collection Information Document
B -1
June 2010
-------
Air Filters — Qualitative Determination
Analyte
lodine-131
lridium-192
Molybdenum-99
Polonium-210
Selenium-75
Strontium-90
Technetium-99
Sample Size111
1 filter
1 filter
1 filter
1 filter
1 filter
1 filter
1 filter
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R(9)
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Method 1 1 1
(EPA)(6)
Ga-01-R
(HASL-300)
Sr-03-RC
(HASL-300)
APS
(ORISE)
Sample Collection Information Document
B -2
June 2010
-------
Air Filters — Confirmatory
Analyte
Americium-241
Californium-252
Curium-244
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Molybdenum-99
Plutonium-238
Plutonium-239
Uranium-238
Uranium-234
Uranium-235
Sample Size111
1 filter
1 filter
1 filter
1 filter
1 filter
1 filter
1 filter
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 170 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Am-04-RC
(HASL-300)
Ga-01-R
(HASL-300)
Procedure #9(9)
(ORISE)
Ga-01-R(9)
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
EMSL
pg. 33 (EPA)(7)
Sample Collection Information Document
B -3
June 2010
-------
Air Filters — Confirmatory
Analyte
Polonium-210
Radium-226
Selenium-75
Strontium-90
Technetium-99
Gross Alpha
Sample Size111
1 filter
1 filter
1 filter
1 filter
1 filter
1 L (solids
limited)
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Method 1 1 1
(EPA)(6)
EMSL-pg.19
(EPA)(7)
Ga-01-R
(HASL-300)
Sr-03-RC
(HASL-300)
APS
(ORISE)
7110B(SM)
Sample Collection Information Document
B -4
June 2010
-------
Aqueous & Liquid Phase Samples
Analyte
Gross Beta
Gamma
Sample Size111
1 L (solids
limited)
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container'2'
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'2'
Maximum 170 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation'2'
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'3'
7110B(SM)
Ga-01-R
(HASL-300)
Aqueous & Liquid Phase — Qualitative Determination
Analyte
Americium-241
Californium-252
Curium-244
Plutonium-238
Plutonium-239
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
Sample Size111
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container'2'
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Holding Time'2'
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
-125 of 59.4 days
Sample Preservative or
Preparation'2'
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific-activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'3'
D3084 (ASTM)
7120(SM)
Procedure #9
(ORISE)
Sample Collection Information Document
B -5
June 2010
-------
Aqueous & Liquid Phase — Qualitative Determination
Analyte
lodine-131
lridium-192
Molybdenum-99
Polonium-210
Radium-226
Selenium-75
Sample Size111
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
2.5 L
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 8 days
based on half-life of 1
131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
acid to pH < 2. Samples
arriving unpreserved
should be allowed to rest
for 16 hours with
preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
acid to pH < 2. Samples
arriving unpreserved
should be allowed to rest
for 16 hours with
preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R
(HASL-300)
7120(SM)
Ga-01-R
(HASL-300)
Po-02-RC
(HASL-300)
7500-Ra B (SM)
7120(SM)
Sample Collection Information Document
B -6
June 2010
-------
Aqueous & Liquid Phase — Qualitative Determination
Analyte
Strontium-90
Technetium-99
Tritium (Hydrogen-3)
Uranium-238
Uranium-234
Uranium-235
Sample Size111
0.5L-4L
1 L
1 L
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
7500-Sr B (SM)
Tc-02-RC
(HASL-300)
906.0 (EPA)(8)
7500-U B (SM)
Aqueous & Liquid Phase — Confirmatory
Analyte
Americium-241
Californium-252
Curium-244
Sample Size111
0.1 -1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Am-04-RC
(HASL-300)
Sample Collection Information Document
B -7
June 2010
-------
Aqueous & Liquid Phase — Confirmatory
Analyte
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Plutonium-238
Plutonium-239
Molybdenum-99
Sample Size111
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 6 months
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
7120(SM)
Procedure #9
(ORISE)
Ga-01-R
(HASL-300)
7120(SM)
EMSL
pg. 33 (EPA)(7)
Ga-01-R
(HASL-300)
Sample Collection Information Document
June 2010
-------
Aqueous & Liquid Phase — Confirmatory
Analyte
Polonium-210
Radium-226
Selenium-75
Strontium-90
Technetium-99
Sample Size111
2.5 L
0.5L-4L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
0.5L-4L
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
acid to pH < 2. Samples
arriving unpreserved
should be allowed to rest
for 16 hours with
preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific-activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific-activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Po-02-RC
(HASL-300)
7500-Ra C (SM)
7120(SM)
7500-Sr B (SM)
Tc-02-RC
(HASL-300)
Sample Collection Information Document
B -9
June 2010
-------
Aqueous & Liquid Phase — Confirmatory
Analyte
Tritium (Hydrogen-3)
Uranium-238
Uranium-234
Uranium-235
Sample Size111
1 L
1 L
Sample Container121
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
906.0 (EPA)(8)
7500-U C (SM)
Drinkinq Water Samples
Analyte
Gross Beta
Gross Beta
Gamma
Sample Size111
1 L (solids
limited)
1 L (solids
limited)
Volume
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container'2'
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
900.0 (EPA)(8)
900.0 (EPA)(8)
901.1 (EPA)(8)
Sample Collection Information Document
B-10
June 2010
-------
Drinking Water — Qualitative Determination
Analyte
Americium-241
Californium-252
Curium-244
Plutonium-238
Plutonium-239
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Molybdenum-99
Sample Size111
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
D3084 (ASTM)
901.1 (EPA)(8)
Procedure #9
(ORISE)
901.1 (EPA)(8)
901.1 (EPA)(8)
901.1 (EPA)(8)
Sample Collection Information Document
B-11
June 2010
-------
Drinking Water — Qualitative Determination
Analyte
Polonium-210
Radium-226
Selenium-75
Strontium-90
Technetium-99
Tritium (Hydrogen-3)
Sample Size111
2.5 L
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
0.5L-4L
1 L
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
acid to pH < 2. Samples
arriving unpreserved
should be allowed to rest
for 16 hours with
preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific-activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Po-02-RC
(HASL-300)
903.0 (EPA)(8)
901.1 (EPA)(8)
7500-Sr B (SM)
Tc-02-RC
(HASL-300)
906.0 (EPA)(8)
Sample Collection Information Document
B-12
June 2010
-------
Drinking Water — Qualitative Determination
Analyte
Uranium-238
Uranium-234
Uranium-235
Sample Size111
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
908.0 (EPA)(8)
Drinking Water — Confirmatory
Analyte
Americium-241
Californium-252
Curium-244
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Sample Size111
0.1 -1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
-125 of 59.4 days
Maximum 8 days
based on half-life of
-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Am-04-RC
(HASL-300)
901.1 (EPA)(8)
Procedure #9
(ORISE)
901.1 (EPA)(8)
901.1 (EPA)(8)
Sample Collection Information Document
B-13
June 2010
-------
Drinking Water — Confirmatory
Analyte
Molybdenum-99
Plutonium-238
Plutonium-239
Polonium-210
Radium-226
Selenium-75
Sample Size111
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
1 L
2.5 L
1 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
acid to pH < 2. Samples
arriving unpreserved
should be allowed to rest
for 16 hours with
preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
901.1 (EPA)(8)
EMSL
pg. 33 (EPA)(7)
Po-02-RC
(HASL-300)
903.1 (EPA)(8)
901.1 (EPA)(8)
Sample Collection Information Document
B-14
June 2010
-------
Drinking Water — Confirmatory
Analyte
Strontium-90
Technetium-99
Tritium (Hydrogen-3)
Uranium-238
Uranium-234
Uranium-235
Sample Size111
0.5L-4L
1 L
1 L
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
No preservation required.
Preserve sample with nitric
or hydrochloric acid to pH
< 2. Samples arriving
unpreserved should be
allowed to rest for 16 hours
with preservative prior to
sample preparation, time
permitting.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
7500-Sr B (SM)
Tc-02-RC
(HASL-300)
906.0 (EPA)(8)
D3972 (ASTM)
Soil & Sediment Samples
Analyte
Gross Alpha
Sample Size111
0.1 g
Sample Container'2'
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
AP1 (ORISE)
Sample Collection Information Document
B-15
June 2010
-------
Soil & Sediment Samples
Analyte
Gross Beta
Gamma
Sample Size111
0.1 g
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh.
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
AP1 (ORISE)
Ga-01-R
(HASL-300)
Soil & Sediment — Qualitative Determination
Analyte
Americium-241
Californium-252
Curium-244
Radium-226
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
Sample Size111
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
1 kg
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container'2'
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Am-02-RC
(HASL-300)
D3084 (ASTM)
Ga-01-R
(HASL-300)
Sample Collection Information Document
B-16
June 2010
-------
Soil & Sediment — Qualitative Determination
Analyte
lodine-125
lodine-131
lridium-192
Molybdenum-99
Plutonium-238
Plutonium-239
Polonium-210
Sample Size111
10g
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
10g
1-5g
Sample Container121
Borosilicate glass
wide mouth jar.
Borosilicate glass
wide mouth jar.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Procedure #9
(ORISE)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
D3084 (ASTM)
Po-02-RC
(HASL-300)
Sample Collection Information Document
B-17
June 2010
-------
Soil & Sediment — Qualitative Determination
Analyte
Selenium-75
Strontium-90
Technetium-99
Tritium (Hydrogen-3)
Uranium-238
Uranium-234
Uranium-235
Sample Size111
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Based on gross
beta analysis,
weigh out
sufficient sample
to obtain 10-100
times background
activity
10g
50 g
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Borosilicate glass
wide mouth jar.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
No preservation required.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R
(HASL-300)
Sr-03-RC
HASL-300)
APS
(ORISE)
AP2
(ORISE)
D3084 (ASTM)
Sample Collection Information Document
B-18
June 2010
-------
Soil & Sediment — Confirmatory
Analyte
Americium-241
Californium-252
Curium-244
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Molybdenum-99
Sample Size111
1 kg
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
10g
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Sample Container121
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Borosilicate glass
wide mouth jar.
Borosilicate glass
wide mouth jar.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh. Samples with
organic contamination may
require muffling at 500°C
for 4 hours.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Am-01-RC
(HASL-300)
Ga-01-R
(HASL-300)
Procedure #9
(ORISE)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Sample Collection Information Document
B-19
June 2010
-------
Soil & Sediment — Confirmatory
Analyte
Plutonium-238
Plutonium-239
Polonium-210
Radium-226
Selenium-75
Strontium-90
Sample Size111
1 kg
1-5g
19
Volume or mass
equivalent to
calibration
standard, approx.
0.5-4 L
Based on gross
beta analysis,
weigh out
sufficient sample
to obtain 10-100
times background
activity
Sample Container121
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Plastic
(polypropylene or
polyethylene) or
borosilicate glass
small-mouth bottle
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh. Samples with
organic contamination may
require muffling at 500°C
for 4 hours.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°Cfor4
hours and grinding or
sieving to a minimum 100
mesh. Samples with
organic contamination may
require muffling at 500°C
for 4 hours.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Wrap glass containers with bubble wrap.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
EMSL
pg. 33 (EPA)(7)
Po-02-RC
(HASL-300)
EMSL
pg.19(EPA)(7)
Ga-01-R
(HASL-300)
Sr-03-RC
(HASL-300)
Sample Collection Information Document
B-20
June 2010
-------
Soil & Sediment — Confirmatory
Analyte
Technetium-99
Tritium (Hydrogen-3)
Uranium-238
Uranium-234
Uranium-235
Sample Size111
10g
50 g
1 L
Sample Container121
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Borosilicate glass
wide mouth jar.
Plastic
(polypropylene or
polyethylene) large-
mouth bottle, jar, or
plastic bag.
Borosilicate glass
wide mouth jar may
also be used.
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
Homogenize sample at
collection. No preservation
required. Analyze as is
without preparation.
No preservation required.
Homogenize sample at
collection. No preservation
required. Prepare sample
by drying at 105°C for 4
hours and grinding or
sieving to a minimum 100
mesh. Samples with
organic contamination may
require muffling at 500°C
for 4 hours.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe sample containers with a clean,
damp cloth and seal with tape. Wrap glass
containers in bubble wrap. Determine the
radiation-specific activity of the sample(s)
and pack samples outside the
contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
APS
(ORISE)
AP2
(ORISE)
EMSL
pg.33 (EPA)(6)
Surface Wipes
Analyte
Gross Alpha
Gross Beta
Sample Size111
1 wipe (limited to
counting
geometry size)
1 wipe (limited to
counting
geometry size)
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 6 months
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Vol. 2, pg. 33
(FRMAC)
Vol. 2, pg. 33
(FRMAC)
Surface Wipes — Qualitative Determination
Analyte
Gamma
Sample Size111
1 wipe (limited to
counting
geometry size)
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R
(HASL-300)
Sample Collection Information Document
B-21
June 2010
-------
Surface Wipes — Qualitative Determination
Analyte
Americium-241
Californium-252
Curium-244
Plutonium-238
Radium-226
Uranium-238
Uranium-234
Uranium-235
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
lodine-125
lodine-131
lridium-192
Molybdenum-99
Plutonium-239
Sample Size111
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
Twelve 2" wipes
or three 4" wipes
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 6 months
Maximum 6 months
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
D3084 (ASTM)
Ga-01-R
(HASL-300)
Procedure #9
(ORISE)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
D3084 (ASTM)
Sample Collection Information Document
B-22
June 2010
-------
Surface Wipes — Qualitative Determination
Analyte
Polonium-210
Selenium-75
Strontium-90
Americium-241
Californium-252
Curium-244
Technetium-99
Tritium (Hydrogen-3)
Sample Size111
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 6 months
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Method 1 1 1
(EPA)(6)
Ga-01-R
(HASL-300)
Sr-03-RC
(HASL-300)
Am-04-RC
(HASL-300)
APS
(ORISE)
AP2
(ORISE)
Surface Wipes — Confirmatory
Analyte
Cesium-137
Cobalt-60
Europium-154
Ruthenium-103
Ruthenium-106
Sample Size111
1 wipe
Sample Container'2'
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R
(HASL-300)
Sample Collection Information Document
B-23
June 2010
-------
Surface Wipes — Confirmatory
Analyte
lodine-125
lodine-131
lridium-192
Molybdenum-99
Plutonium-238
Plutonium-239
Uranium-238
Uranium-234
Uranium-235
Polonium-210
Radium-226
Sample Size111
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
1 wipe
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 59 days
based on half-life of
1-1 25 of 59.4 days
Maximum 8 days
based on half-life of
1-131 of 8.03 days
Maximum 1 70 days
based on half-life of
lr-192of 173 days
Maximum 65 hours
based on half-life of
Mo-99 of 65.94 hours
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 1 73. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Procedure #9
(ORISE)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
Ga-01-R
(HASL-300)
EMSL - pg. 33
(EPA(7))
Method 1 1 1
(EPA)(6)
EMSL
pg. 19(EPA)(7)
Sample Collection Information Document
B-24
June 2010
-------
Surface Wipes — Confirmatory
Analyte
Selenium-75
Strontium-90
Technetium-99
Tritium (Hydrogen-3)
Sample Size111
1 wipe
1 wipe
1 wipe
1 wipe
Sample Container121
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Glassine envelope,
polyacrylic plastic
Petri dish, or
polyethylene plastic
bag
Holding Time'21
Maximum 120 days
based on half-life of
Se-75of 120 days
Maximum 6 months
Maximum 6 months
Maximum 6 months
Sample Preservative or
Preparation121
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Analyze as is without
preparation.
No preservation required.
Packaging Requirements
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Wipe outside of each container clean
using a damp, then dry cloth. Seal the
container with non-reactive tape or film.
Determine the radiation-specific activity of
the sample(s) and pack samples outside
the contaminated area as described in
Footnote (4).
Shipping Label
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Shipping labels per requirements of
49 CFR 173. Determine the
radiation levels on the surface and
at 1 meter from the surface of the
package, and label. Refer to
information in Footnote (5).
Source/SAM
Method'31
Ga-01-R
(HASL-300)
Sr-03-RC
(HASL-300)
APS
(ORISE)
AP2
(ORISE)
Footnotes:
(1) The sample sizes listed are based on the amount needed for analysis of a single sample. If requested by the laboratory, additional samples(s) must be collected for laboratory quality control analyses (e.g.,
duplicates, matrix spikes). It is also recommended that additional sample(s) be collected in case of the need for reanalysis due to sample spillage or unforseen analytical difficulties.
(2) Sample container, holding times, preservation, and sample preparation information has been derived from requirements stated in the analytical methods or documents cited under "SAM/Source Method" or in the
documents cited in Footnotes 6 through 8.
(3) SAM methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center (NHSRC), Standardized Analytical Methods for Environmental
Restoration following Homeland Security Events, Revision 5.0, September 2009 (www.epa.gov/sam/).
(4) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers. Samples should be surrounded by shock
absorbing and liquid absorbent packing materials (49CFR 173.24 and 173.453). Check the radiation-specific activity from the packaged samples (49 CFR 173.403).
(5) Although the package required for transporting radioactive material is based on the activity INSIDE the package, the label required on the transport package is based on the radiation hazard OUTSIDE the
package. Radioactive material is the only hazardous material that has three possible labels, depending on the relative radiation levels external to the package. Labels for radioactive material also require a Tl
number which indicates the highest radiation level measured at 1 meter from the surface of the package. The three possible labels are commonly called White 1, Yellow 2, and Yellow 3, referring to the color of the
label and the number prominently displayed. A specific label is required if the surface radiation limit and the limit at 1 meter satisfy the requirements of 49 CFR 172.403.
(6) 40 CFR 61, Appendix B.
(7) EMSL LV053917 Radiochemical Analytical Procedures for Analysis of Environmental Samples, March 1979, US Department of Energy.
(8) EPA 600/4-80-032 Prescribed Procedures for Measurement of Radioactivity in Drinking Water, August 1980, US Environmental Protection Agency.
(9) This procedure should be used only for filters specifically designed for iodine.
Sample Collection Information Document
B-25
June 2010
-------
Attachment C
Sample Collection Information for the Pathogens
Listed in SAM
Sample Collection Information Document June 2010
-------
Attachment C: Sample Collection Information for the Pathogens Listed in SAM
Solid (Soil, Powder) — Bacteria
Analyte(s)
Bacillus anthracis
[Anthrax]
Brucella spp.
[Brucellosis]
Burkholderia mallei
[Glanders]'51
Burkholderia
pseudomallei
[Melioidosis]'51
Campylobacterjejuni
[Campylobacteriosis]'5'
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
50- 100 g
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label'31
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Public Health Reports. 92(2): 176-186
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 G
Sample Collection Information Document
C1 -1
June 2010
-------
Solid (Soil, Powder) — Bacteria
Analyte(s)
Chlamydophila psittaci
(formerly known as
Chlamydia psittaci )
[Psittacosis]'51
Coxiella bumetii
[Q-fever](51
Escherichia coli
0157:H7(S|
Francisella tularensis
[Tularemia]'51
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Sample Size111
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Journal of Clinical Microbiology. 38:
10-1093
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sample Collection Information Document
C1 -2
June 2010
-------
Solid (Soil, Powder) — Bacteria
Analyte(s)
Leptospira spp.
(L. interrogans serovars:
L. icteroheamorrhagiae,
L. autralis, L. balum, L.
bataviae, L. sejro, L.
pomona)
[Leptospirosis]
Listeria monocytogenes
[Listeriosis]'51
Non-typhoidal
Salmonella
[Salmonellosis]'51
Salmonella Typhi
[Typhoid fever]'51
Container
Small,
tightly
sealed
sterile bottle
or plastic
bag. A
small
amount of
sterile
de ionized
water may
be added to
prevent
drying.
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
A small amount of
sterile deionized
water should be
present in
container to
prevent drying.
Room temperature
within 72 hours of
collection; if
longer, keep on
ice packs (or
secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice). If
sample is already
frozen, do not
thaw until
analysis.
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
10-20g
At least 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
possible, analyze
sample within 72
hours of collection.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 I
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
FDA/Bacteriological Analytical Manual,
Ch. 1 &1 0,2003
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060
Sample Collection Information Document
C1 -3
June 2010
-------
Solid (Soil, Powder) — Bacteria
Analyte(s)
Shigella spp.
[Shigellosis]'51
Staphylococcus
aureus{i}
Vibrio cholerae 01 and
O139
[Cholera]'51
Yersinia pestis
[Plague]'51
Container
Sterile
plastic bags
or glass or
plastic
bottles
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice (e.g.,
ice packs, secure
double-bagged
ice) if longer.
Store at room
temperature. Do
not ship on ice.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Sample Size'11
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Process samples as
soon as possible
after collection
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laborator.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory
Packaging Requirements'21
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label'31
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substancess
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Standard Methods 9060 and 9260 E
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Current Protocols in Microbiology Chapter
6: 6A.5.1 - 6A.5.38
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sample Collection Information Document
C1 -4
June 2010
-------
Solid (Soil, Powder) — Viruses
Analyte(s)
Adenovi ruses:
Enteric and non-enteric
(A-F)<5>
Astroviruses151
Caliciviruses:
Norovirus151
Caliciviruses:
Sapo virus151
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
Sample Collection Information Document
C1 -5
June 2010
-------
Solid (Soil, Powder) — Viruses
Analyte(s)
Coronaviruses: SARS-
associated human
coronavirus151
Hepatitis E virus (HEV)'51
Influenza H5N1 virus'51
Picornaviruses:
Enteroviruses151
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006;
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sample Collection Information Document
C1 -6
June 2010
-------
Solid (Soil, Powder) — Viruses
Analyte(s)
Picornaviruses:
Hepatitis A virus (HAV)'51
Reo viruses:
Rotavirus (Group A)
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
50- 100 g
50- 100 g
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for Virology,
Ch. 14, 2006
Solid (Soil, Powder) — Protozoa
Analyte(s)
Cryptosporidium spp.
[Cryptosporidiosis]
Container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Sample Size111
50- 100 g
Holding Time
Samples must be
shipped via
overnight service on
the day they are
collected. Sample
processing should
be completed as
soon as possible by
the lab. Sample
elution must be
initiated within 96
hours of sample
collection or
filtration.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1622 and 1623, EPA-821-
R-01-026, 2001
Sample Collection Information Document
C1 - 7
June 2010
-------
Solid (Soil, Powder) — Protozoa
Analyte(s)
Entamoeba /i/sto/ytfca '*'
Giardia spp.
[Giardiasis]'51
Toxoplasma gondii
[Toxoplasmosis]151
Container
Sterile,
sealed,
leakproof
container
Sterile,
leakproof
container
Sterile,
sealed,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Sample Size111
50- 100 g
50- 100 g
50- 100 g
Holding Time
Transport specimen
as soon as
possible. If
transport is delayed
over one hour,
refrigerate
specimen.
Samples must be
shipped via
overnight service on
the day they are
collected. Sample
processing should
be completed as
soon as possible by
the lab. Sample
elution must be
initiated within 96
hours of sample
collection or
filtration.
Transport specimen
as soon as
possible. If
transport is delayed
over one hour,
refrigerate
specimen.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1622 and 1623, EPA-821-
R-01-026, 2001
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1623, EPA-821-R-01-026,
2001
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1622 and 1623, EPA-821-
R-01-026, 2001
Solid (Soil, Powder) — Helminthes
Analyte(s)
Baylisascaris procyonis
[Raccoon round worm
infection]
Container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice). Store
at 2 - 5°C at
laboratory; do not
freeze samples.
Sample Size111
300 - 600 g
Holding Time
Ship to laboratory
for analysis within
24 hours of sample
collection.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air)
with list of contents, shipper, and
recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B - Infectious
Substances
DOT, PHMSA, Transporting Infectious
Substances Safely
Environmental Regulations and
Technology: Control of Pathogens and
Vector Attraction in Sewage Sludge,
Appendix I (2003). EPA/625/R92/013
Sample Collection Information Document
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Bacteria
Analyte(s)
Bacillus anthracis
[Anthrax]
Brucella spp.
[Brucellosis]'51
Burkholderia mallei
[Glanders]'51
Burkholderia
pseudomallei
[Melioidosis]'51
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Room temperature if
held for 1 hour or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements'21
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label'31
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
Sample Collection Information Document
C2-1
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Bacteria
Analyte(s)
Campy/o/3 acterjejuni
[Campylobacteriosis]151
Chlamydophila psittaci
(formerly known as
Chlamydia psittaci )
[Psittacosis]'51
Coxiella burnetii
[Q-fever](51
Escherichia coli
0157.-H7™
Francisella tularensis
[Tularemia]151
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Room temperature if
held for 1 hour or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Journal of Clinical Microbiology. 38:
10-1093
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
Sample Collection Information Document
C2-2
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Bacteria
Analyte(s)
Leptospira spp.
(L. interrogans serovars:
L. icteroheamorrhagiae,
L. autralis, L. balum, L.
bataviae, L. sejro, L.
pomona )
[Leptospirosis]'5'
Listeria monocytogenes
[Listeriosis]151
Non-typhoidal Salmonella
[Salmonellosis]
Salmonella Typhi
[Typhoid Fever]'51
Shigella spp.
[Shigellosis]'51
Container
Sterile,
leakproof
container. A
small
amount of
sterile
de ionized
water may
be added to
prevent
drying.
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
A small amount of
sterile deionized
water should be
present in container
to prevent drying.
Ambient temperature
within 72 hours of
collection; if longer,
keep on ice packs (or
secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice). If
sample is frozen, do
not thaw until
analysis.
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
possible analyze
sample within 72
hours of
collection.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Process samples
as soon as
possible after
collection.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
FDA/Bacteriological Analytical
Manual, Ch. 1 & 10, 2003
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
Sample Collection Information Document
C2-3
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Bacteria
Analyte(s)
Staphylococcus aureus'5'
Vibrio cholerae 01 and
O1 39 [Cholera]'51
Yersinia pestis
[Plague]'51
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Store at room
temperature. Do not
ship on ice.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if longer.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements'21
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label'3'
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'4'
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Standard Methods 9060 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Current Protocols in Microbiology
Chapter 6: 6A.5.1 - 6A.5.38
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA, Draft Environmental
Sampling Guidelines and Analytical
Approach for Biological Response
Plans, 2006
Particulate (Swab, Wipe, Dust Socks) — Viruses
Analyte(s)
Adenoviruses:
Enteric and non-enteric
(A-F)<5>
Astro viruses'5'
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label'3'
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'4'
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
Sample Collection Information Document
C2-4
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Viruses
Analyte(s)
Caliciviruses: Norovirus'5'
Caliciviruses: Sapovirus'5'
Coronaviruses: SARS-
associated human
coronavirus151
Hepatitis E virus (HEV)'51
Influenza H5N1 virus'51
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
Sample Collection Information Document
C2-5
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Viruses
Analyte(s)
Picornavi ruses:
Entero viruses'5'
Picornavi ruses:
Hepatitis A virus (HAV)'5'
Reoviruses:
Rotavirus (Group A)'5'
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label13'
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'4'
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
Particulate (Swab, Wipe, Dust Socks) — Protozoa
Analyte(s)
Cryptosporidium spp.
[Cryptosporidiosis]151
Container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice); do not
freeze.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Samples must be
shipped via
overnight service
on the day they
are collected.
Sample
processing should
be completed as
soon as possible.
Sample elution
must be initiated
within 96 hours of
sample collection
or filtration.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label13'
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'4'
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Method 1622 and 1623,
EPA-821-R-01 -026, 2001
Sample Collection Information Document
C2-6
June 2010
-------
Particulate (Swab, Wipe, Dust Socks) — Protozoa
Analyte(s)
Entamoeba histolytica '5'
Giardia spp.
[Giardiasis]'51
Toxoplasma gondii
[Toxoplasmosis]151
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice); do not
freeze.
Keep on ice packs
(or secure double-
bagged ice); do not
freeze.
Keep on ice packs
(or secure double-
bagged ice); do not
freeze.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Transport
specimen as soon
as possible. If
transport is
delayed over one
hour, refrigerate
specimen.
Samples must be
shipped via
overnight service
on the day they
are collected.
Sample
processing should
be completed as
soon as possible.
Sample elution
must be initiated
within 96 hours of
sample collection
or filtration.
Transport sample
as soon as
possible. If
transport is
delayed over one
hour, refrigerate
sample.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Method 1622 and 1623,
EPA-821-R-01 -026, 2001
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Method 1623, EPA-821-R-
01-026, 2001
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
USEPA Method 1622 and 1623,
EPA-821-R-01 -026, 2001
Particulate (swab, wipe, dust socks) — Helminthes
Analyte(s)
Baylisascaris procyonis '5'
[Raccoon roundworm
inffection]
Container
Sterile,
leakproof
container
Preservation
Keep on ice packs
(or secure double-
bagged ice). Store at
2 - 5°C at laboratory.
Do not freeze
samples.
Sample Size
At least 2 sterile,
synthetic, and
moistened wipes
or swabs, or dust
socks
Holding Time
Ship to laboratory
for analysis within
24 hours of
sample collection.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper, and
recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely, 2006
Environmental Regulations and
Technology: Control of Pathogens
and Vector Attraction in Sewage
Sludge, Appendix I (2003).
EPA/625/R92/013
Sample Collection Information Document
C2- 7
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Bacillus anthracis
[Anthrax]
Brucella spp.
[Brucellosis]
Burkholderia mallei
[Glanders]
Burkholderia
pseudomallei
[Melioidosis]'51
Campylobacterjejuni
[Campylobacteriosis]
i/ater(e| (filter, a
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
rab^ — Bacterial
Preservation
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
A minimum of
200 mL
A minimum of
100mL
A minimum of
100mL
A minimum of
100ml
1 to several
liters; 1 L for
turbid waters
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Process sample
immediately after
collection or store
and process as
soon as possible.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Public Health Reports. 92(2): 176-186
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 G
Sample Collection Information Document
C3-1
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Chlamydophila
psittaci (formerly
known as Chlamydia
psittaci) [Psittacosis]'51
Coxiella bumetii
[Q-fever](51
Escherichia coli
0157:H7
Francisella tularensis
[Tularemia]
i/ater'61 (filter, a
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
rab^ — Bacterial
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Keep on ice (e.g.,
ice packs, secure
double-bagged
ice) if longer.
Room temperature
if held for 1 hour
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Sample Size111
A minimum of
100mL
A minimum of
100mL
A minimum of
100ml
A minimum of
100ml
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sample Collection Information Document
C3-2
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Leptospira spp.
(L. interrogans
serovars: L.
icteroheamorrhagiae,
L. autralis, L. balum, L.
bataviae, L. sejro, L.
pomona)
[Leptospirosis]
Listeria
monocytogenes
[Listeriosis]'51
Non-typhoidal
Salmonella
[Salmonellosis]
Salmonella Typhi
[Typhoid fever]
i/ater(e| (filter, a
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
rab^ — Bacterial
Preservation
A small amount of
sterile deionized
water should be
present in
container to
prevent drying.
Ambient
temperature within
72 hours of
collection; if
longer, keep on
ice packs (or
secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice). If
sample is already
frozen do not thaw
until analysis.
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
100mL to 1 L;
multiple samples
are usually
required; agitate
water to bring
sediment to
surface to collect
50-100mL
Cheese cloth
swab; or 2 L
water for
diatomaceous
earth filter, 20 L
for borosilicate
glass filtration,
or at least 1 L
for membrane
filtration
Cheese cloth
swab; or 2 L
water for
diatomaceous
earth filter, 20 L
for borosilicate
glass filtration,
or at least 1 L
for membrane
filtration
Holding Time
If possible,
analyze sample
within 72 hours of
collection.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Maximum
transport and
storage time
allowable is 6
hours.
Maximum
transport and
storage time
allowable is 6
hours.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 I
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
FDA/Bacteriological Analytical Manual,
Ch. 1 & 10, 2003
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 B
Sample Collection Information Document
C3-3
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Shigella spp.
[Shigellosis]
Staphylococcus
aureus
Vibrio cholerae 01 and
0139
[Cholera]
Yersinia pestis
[Plague]
i/ater'61 (filter, a
Container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
Sterile,
leakproof
container
rab^ — Bacterial
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Store at room
temperature. Do
not ship on ice.
Room temperature
if held for 2 hours
or less; keep on
ice (e.g., ice
packs, secure
double-bagged
ice) if longer.
Sample Size111
1 L
A minimum of
100mL
Moore swab, or
a minimum of
100mL
A minimum of
100mL
Holding Time
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Minimize
transport and
storage time. If
feasible, analyze
or extract
immediately upon
receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9260 E
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Standard Methods 9060 and 9213 B
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Current Protocols in Microbiology
Chapter 6: 6A.5.1 - 6A.5.38
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sample Collection Information Document
C3-4
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Adenovi ruses:
Enteric and non-
enteric (A-F)(S|
Astroviruses151
Caliciviruses:
Norovirus'5'
Caliciviruses:
Sapo virus'5'
i/ater(61 (filter, a
Container
Positively
charged
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
rab^ — Viruses
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
Filter apparatus
should be
allowed to run
overnight.
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200 - 300 L
surface/
recreational
water
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
Holding Time
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Packaging Requirements121
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
Sample Collection Information Document
C3-5
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Coronaviruses: SARS-
associated human
coronavirus151
Hepatitis E virus
(HEV)'51
Influenza H5N1 virus'51
Picornaviruses:
Enteroviruses151
i/ater'61 (filter, a
Container
Positively
charged
1MDS
cartridge filter
Double layer
142 mm
diameter
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
rab^ — Viruses
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
10 L surface
water
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
recreational
water'3'
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
Filter apparatus
should be
allowed to run
overnight
Holding Time
Filters should be
eluted within 24
hours of start of
the sample
collection, and
must be eluted
within 72 hours of
start of the
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
start of the
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
start of the
sample collection.
Packaging Requirements121
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Applied and Environmental
Microbiology. 69(6): 3158-3164
USEPA Manual of Methods for
Virology, Ch. 14,2006
Sample Collection Information Document
C3-6
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Picornaviruses:
Hepatitis A virus
(HAV)'51
Reo viruses:
Rotavirus (Group A)'51
Linuiri/Water/Drinkinn V
Analyte(s)
Cryptosporidium spp.
[Cryptosporidiosis]
i/ater'61 (filter, a
Container
Positively
charged
1MDS
cartridge filter
Positively
charged
1MDS
cartridge filter
i/ater(61 (filter, a
Container
Sterile
leakproof
containers
OR
Filter in sterile
leakproof
container
rab^ — Viruses
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
Filter apparatus
should be
allowed to run
overnight
1 500- 2000 L
drinking water/
ground water;
2-20L
wastewater;
200- 300 L
surface/
recreational
water
Filter apparatus
should be
allowed to run
overnight
Holding Time
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Filters should be
eluted within 24
hours of the start
of sample
collection, and
must be eluted
within 72 hours of
the start of
sample collection.
Packaging Requirements121
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Cover ends of filter module with
aluminum foil and place in labeled, sealed,
decontaminated, leakproof primary sample
container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Applied and Environmental
Microbiology. 69(6): 3158-3164
USEPA Manual of Methods for
Virology, Ch. 14,2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Applied and Environmental
Microbiology. 69(6): 3158-3164
(Culture)
Journal of Virological Methods. 155(2):
126-1 31 (PCR)
USEPA Manual of Methods for
\/:..AIA~.,, ou -t A onno
rab> — Protozoa
Preservation
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Sample Size111
10-15L
Holding Time
Samples must be
shipped via
overnight service
on the day of
collection.
Sample
processing should
be completed as
soon as possible.
Sample elution
must be initiated
within 96 hours of
sample collection
or filtration.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1623, EPA-821-R-01-
026, 2001
Sample Collection Information Document
C3- 7
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Entamoeba
histolytica(y>
Giardia spp.
[Giardiasis]
Toxoplasma gondii
[Toxoplasmosis]'5'
i/ater(61 (filter, a
Container
Polypropylene
barrels
Polypropylene
barrels
OR
Filter in sterile
leakproof
container
Sterile
leakproof
containers
OR
Filter in sterile
leakproof
container
OR
Polypropylene
barrels
rab^ — Protozqg
Preservation
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Keep on ice packs
(or secure double-
bagged ice); do
not freeze.
Sample Size111
1 00 L into ten 10
L containers
1 00 L into ten 10
L containers
OR
Up to 1000L
through filter
cartridge
1 00 L into ten 10
L containers
OR
4650 L for filter
cartridge
Holding Time
Samples must be
shipped via
overnight service
on the day of
collection.
Sample
processing should
be completed as
soon as possible.
Samples must be
shipped via
overnight service
on the day of
collection.
Sample
processing should
be completed as
soon as possible.
Sample elution
must be initiated
within 96 hours of
sample collection
or filtration.
Samples must be
shipped via
overnight service
on the day of
collection.
Sample
processing should
be completed as
soon as possible.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents; or cover ends of filter
module with aluminum foil and place in
labeled, sealed, decontaminated, leakproof
primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents; or cover ends of filter
module with aluminum foil and place in
labeled, sealed, decontaminated, leakproof
primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents; or cover ends of filter
module with aluminum foil and place in
labeled, sealed, decontaminated, leakproof
primary sample container
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper,
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Applied and Environmental
Microbiology. 70(7): 4035-4039
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
USEPA Method 1623, EPA-821-R-01-
026, 2001
Applied and Environmental
Microbiology. 70(7): 4035-4039
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Applied and Environmental
Microbiology. 70(7): 4035-4039
Emerging Infectious Diseases. 12(2):
326-329
Sample Collection Information Document
June 2010
-------
Linuiri/Water/Drinkinn V
Analyte(s)
Baylisascaris
procyonis
[Raccoon round worm
infection]'51
i/ater'61 (filter, a
Container
Sterile
leakproof
container
rabl — Helminthes
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Store at 2 - 5°C at
laboratory. Do not
freeze samples.
Sample Size'11
At least 1 L
Holding Time
Ship to laboratory
for analysis within
24 hours of
sample collection.
Packaging Requirements'21
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material
to absorb contents
(2) Leakproof secondary container
(capable of withstanding an internal
pressure of 14 psi if shipped by air) with
list of contents, shipper, and recipient
(3) Rigid outer packaging
Shipping Label'31
Label outer packaging with:
(1) Biological Substance, Category B
(2) Full name and address of shipper
and recipient
(3) Name and telephone number of
responsible person (shipper)
Attach diamond-shaped UN# 3373 label
Source/SAM Method'41
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting Infectious
Substances Safely, 2006
Environmental Regulations and
Technology: Control of Pathogens and
Vector Attraction in Sewage Sludge,
Appendix I (2003). EPA/625/R92/013
Sample Collection Information Document
C3-9
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Bacteria
Analyte(s)
Bacillus anthracis
[Anthrax]
Brucella spp.
[Brucellosis]
Burkholderia mallei
[Glanders]'51
Burkholderia
pseudomallei
[Melioidosis]'51
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room temperature if
held for 15 minutes
or less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room temperature if
held for 15 minutes
or less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room temperature if
held for 15 minutes
or less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size'11
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient.
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
Sample Collection Information Document
C4-1
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Bacteria
Analyte(s)
Campylobacterjejuni
[Campylobacteriosis]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Chlamydophila psittaci
(formerly known as
Chlamydia psittaci )
[Psittacosis]'51
Coxiella bumetii
[Q-fever]'51
Escherichia coli
0157:H7
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Keep on ice (e.g.,
ice packs, secure
double-bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Room temperature if
held for 15 minutes
or less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Keep on ice (e.g.,
ice packs, secure
double-bagged ice).
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container,
wrapped with enough absorbent material to
absorb contents
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 2060
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060
Sample Collection Information Document
C4-2
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Bacteria
Analyte(s)
Francisella tularensis
[Tularemia]
Leptospira spp.
(L. interrogans
serovars: L.
icteroheamorrhagiae,
L. autralis, L. balum, L.
bataviae, L. sejro, L.
pomona)
[Leptospirosis]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Listeria
monocytogenes
[Listeriosis]151
Non-typhoidal
Salmonella
[Salmonellosis]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Keep on ice (e.g.,
ice packs, secure
double-bagged ice) .
Keep on ice packs
(or secure double-
bagged ice). If
sample is already
frozen do not thaw
until analysis.
Room temperature if
held for 15 minutes
or less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, May 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060 and 9260
I
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
FDA/Bacteriological Analytical
Manual, Ch. 10, 2003
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060
Sample Collection Information Document
C4-3
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Bacteria
Analyte(s)
Salmonella Typhi
[Typhoid fever]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Shigellaspp.
[Shigellosis]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Staphylococcus
aureus '5'
Vibrio cholerae 01 and
O1 39 [Cholera]
Unlikely to be viable —
samples should be
collected only for PCR
analyses
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter(8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Keep on ice (e.g.,
ice packs, secure
double-bagged ice).
Keep on ice (e.g.,
ice packs, secure
double-bagged ice).
Keep on ice (e.g.,
ice packs, secure
double-bagged ice).
Store at room
temperature. Do not
ship on ice.
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Standard Methods 9060
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
Current Protocols in Microbiology
Chapter 6: 6A.5.1 -6A.5.38
Sample Collection Information Document
C4-4
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Bacteria
Analyte(s)
Yersinia pestis
[Plague]
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA, Draft Environmental
Sampling Guidelines and
Analytical Approach for Biological
Response Plans, 2006
Aerosol (growth media, filter, liguid) — Viruses
Analyte(s)
Adenovi ruses:
Enteric and non-enteric
(A-F)<5>
Astroviruses151
Caliciviruses:
Norovirus151
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements'2'
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
Sample Collection Information Document
C4-5
June 2010
-------
Aerosol (growth media, filter, liquid) — Viruses
Analyte(s)
Caliciviruses:
Sapo virus'5'
Coronaviruses: SARS-
associated human
coronavirus'5'
Hepatitis E virus
(HEV)'51
Influenza H5N1 virus'51
Sampling
Device/Medium
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size'11
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements'21
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label'31
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
Sample Collection Information Document
C4-6
June 2010
-------
Aerosol (Growth Media, Filter, Liquid) — Viruses
Analyte(s)
Picornaviruses:
Enteroviruses'5'
Picornaviruses:
Hepatitis A virus
(HAV)(S|
Reo viruses:
Rotavirus (Group A)|s|
Sampling
Device/Medium
Sterile MCE/PTFE
filter(8), gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Sterile MCE/PTFE
filter'8', gel filter,
impinger, and/or
impactor (agar
plate)
Preservation
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Keep on ice packs
(or secure double-
bagged ice).
Sample Size111
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Depends on method:
120-960Lfor
MCE/PTFE filter; 40
- 1 35 L for gel filter;
750 - 6000 L for
impinger'7'; or 84.9 -
849 L for impactor
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging Requirements121
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Triple packaging consisting of:
(1) Labeled, sealed, decontaminated,
leakproof primary sample container. Place
sealed agar plates in primary container and
ship upside down. For gel filters, add 10
mL sterile distilled water to container to
prevent desiccation.
(2) Leakproof secondary container (capable
of withstanding an internal pressure of 14
psi if shipped by air) with list of contents,
shipper, and recipient
(3) Rigid outer packaging
Shipping Label131
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Label outer packaging with:
(1) Biological Substance,
Category B
(2) Full name and address of
shipper and recipient
(3) Name and telephone
number of responsible
person (shipper)
Attach diamond-shaped UN#
3373 label
Source/SAM Method'41
49 CFR 173.1 99, Category B-
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 173.199, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
49 CFR 1 73.1 99, Category B -
Infectious Substances
DOT, PHMSA, Transporting
Infectious Substances Safely,
2006
USEPA Manual of Methods for
Virology, Ch. 14, 2006
Aerosol (Growth Media, Filter, Liquid) — Protozoa
Cryptosporidium spp.
[Cryptosporidiosis]
Entamoeba histolytica
Giardia spp.
[Giardiasis]
Toxoplasma gondii
[Toxoplasmosis]
Unlikely to be found
Unlikely to be found
Unlikely to be found
Unlikely to be found
Aerosol (Growth Media, Filter, Liquid) — Helminthes
Baylisascaris
procyonis
[Raccoon round worm
infection]
Unlikely to be found
Sample Collection Information Document
C4- 7
June 2010
-------
Footnotes:
(1) The sample sizes listed are based on the amount needed for analysis of a single sample. If requested by the laboratory, additional sample(s) must be collected for laboratory qualitiy control analyses (e.g., duplicates,
matrix spikes). It is also recommended that additional sample(s) be collected in case of the need for reanalysis due to sample spillage or unforseen analytical difficulties.
(2) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample
preservation. Primary receptables should be leakproof with a volumetric capacity of not more than 500 mL (liquid) or 4 kilograms (solid). If several individual primary containers are placed in a single secondary packaging,
they must be individually wrapped or separated so as to prevent contact between them. Secondary packaging should be leakproof and surrounded by shock- and water-absorbent packing materials or ice (if required for
preservation) and shipped in a cooler to ensure sample temperatures do not exceed preservation requirements. Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags
placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 (http://edocket.access.gpo.gov/cfr_2002/octqtr/pdf/49cfr173.199.pdf) and 42 CFR 72 and 73
(http://oig.hhs.gov/authorities/docs/05/032905FRselectagents.pdf).
(3) U.S. DOT and IATA labeling requirements apply to materials that are known to contain, or are suspected of containing, an infectious substance and reflect the most recent changes, effective October 1, 2006. Further
guidance on these changes and lists of substances considered to be either category A (not listed in this document) or category B can be obtained from DOT, PHMSA at
http://www.phmsa.dot.gov/staticfiles/PHMSA/DownloadableFiles/Files/Transporting_lnfectious_Substances_brochure.pdf. Definitions and exceptions for Class 6, Division 6.2 infectious substances are described in 49 CFR
173.134.
(4) SAM methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center (NHSRC), Standardized Analytical Methods for Environmental Restoration
following Homeland Security Events, Revision 5.0, September 2009 (www.epa.gov/sam/).
(5) Currently, no information is available for this analyte in this sample type. Until such time that analye-specific information is available, collection procedures described for a similar analyte/sample type are considered to be
appropriate.
(6) For collection of aqueous samples containing residual chlorine, add a stock solution of filter-sterilized 10% sodium thiosulfate at 0.5 mL/L
(7) If using impingers that do not replenish the liquid as it is evaporated by the air stream, the maximum recommended sampling volume is 200 L (Applied and Environmental Microbiology, Duchaine et al, 2001, 67(6): 2775-
2780).
(8) MCE and PTFE filters are available as cassettes.
Sample Collection Information Document C4 - 8 June 2010
-------
Sample Collection Information Document C4-9 June 2010
-------
Attachment D
Sample Collection Information for the Biotoxin Analytes
Listed in SAM
Sample Collection Information Document June 2010
-------
Attachment D: Sample Collection Information for the Biotoxin Analytes Listed in SAM
Aerosol (Filter/Cassette)'11 — Protein
Analyte(s)
Abrin'51
Botulinum neurotoxins
(Serotoypes A, B, E, F)(s|
Ricin'51
(Ricinine: ricin marker)
Shiga and Shiga-like
Toxins
(Stx, Stx-1,Stx-2)'51
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group III
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN#3172
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN#3172
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN#3172
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D1 -1
June 2010
-------
Aerosol (Filter/Cassette)111 — Protein
Analyte(s)
Staphylococcal
enterotoxins (SEE)'51
Staphylococcal
enterotoxins (SEA,
SEC)'51
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnote Footnotes
(7) and (6).
Belongs to Packing Group I
Shipping Label'31
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
993.06 (AOAC)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
993.06 (AOAC)
Aerosol (Filter/Cassette)'11 — Small Molecule
Analyte(s)
Aflatoxin(TypeBI)'51
a-Amanitin'5'
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store in the
dark at -20°C.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D1 -2
June 2010
-------
Aerosol (Filter/Cassette)111 — Small Molecule
Analyte(s)
Anatoxin-a|51
Brevetoxins
(B form)'51
a-Conotoxin'5'
Cylindrospermopsin'5'
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
-20 °C.
Ship and store at
-20°C.
Ship and store at
negative (-) 20°C.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnote (6).
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnote (6).
Shipping Label'31
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN# 281 1
Label outer package
with the proper
shipping name:
"Toxic solids,
organic, n.o.s." AND
UN# 281 1
This substance is
considered to be non-
hazardous for
transport.
This substance is
considered to be non-
hazardous for
transport.
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D1 -3
June 2010
-------
Aerosol (Filter/Cassette)111 — Small Molecule
Analyte(s)
Diacetoxyscirpenol
(DAS)'51
Microcystin
Isoforms: LA, LR, YR,
LW, RR, YR(S|
Picrotoxin151
Saxitoxins151
Isoforms: Saxitoxin
(SIX), Neosaxitoxin
(NEOSTX), Gonyautoxin
(GTX),
Decarbamoylgonyautoxi
n (dcGTX),
Decarbamoylsaxi toxin
(dcSTX)
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Ship and store in the
dark on ice packs
(or secure double-
bagged ice).
Ship and store at
negative (-) 20°C.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 281 1
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN33172
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D1 -4
June 2010
-------
Aerosol (Filter/Cassette)111 — Small Molecule
Analyte(s)
T-2 Mycotoxin'51
Tetrodotoxin'51
Sampling Device/
Medium
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Sterile MCE or PTFE
filter. Place the filter
in a sterile leakproof
container and seal.
Preservation
Ship and store at
negative (-) 20°C.
Room temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size121
120-960Lfor
MCE or PTFE filter
120-960Lfor
MCE or PTFE filter
Holding Time
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport
and storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Aircraft: cargo only
Decontaminate and label the
exterior of the container. Place in
a cooler and package the samples
outside the contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging for
Solid Hazardous Materials
USEPA, Draft Environmental Sampling Guidelines
and Analytical Approach for Biological Response
Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D1 -5
June 2010
-------
Solid (Soil, Powder) — Protein
Analyte(s)
Abrin'5'
Botulinum neurotoxins
(Serotoypes A, B, E,
F)(5)
Ricin'51
(Ricinine: ricin marker)
Shiga and Shiga-like
Toxins
(Stx, Stx-1 , Stx-2)'51
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
-20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group III
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
U.S. FDA, Bacteriological Analytical Manual
Online, Ch. 17,2001
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D2-1
June 2010
-------
Solid (Soil, Powder) — Protein
Analyte(s)
Staphylococcal
enterotoxins (SEE)'51
Staphylococcal
enterotoxins (SEA,
SEC)'51
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
50- 100 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
993.06 (AOAC)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
993.06 (AOAC)
Solid (Soil, Powder) — Small Molecule
Analyte(s)
Aflatoxin(TypeB1)(51
a-Amanitin'5'
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store in
the dark at -20°C.
Sample Size |21
50 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D2-2
June 2010
-------
Solid (Soil, Powder) — Small Molecule
Analyte(s)
Anatoxin-a'5'
Brevetoxins
(B form)'51
a-Conotoxin'5'
Cylindrospermopsin151
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Ship and store at
negative (-) 20°C.
Ship and store at
negative (-) 20°C.
Sample Size |21
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Shipping Label'31
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 2811
Label outer package with
the proper shipping name:
"Toxic solids, organic,
n.o.s." AND UN# 2811
This substance is
considered to be non-
hazardous for transport
This substance is
considered to be non-
hazardous for transport.
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D2-3
June 2010
-------
Solid (Soil, Powder) — Small Molecule
Analyte(s)
Diacetoxyscirpenol
(DAS)'51
Microcystin
Isoforms: LA, LR, YR,
LW, RR, YR'51
Picrotoxin151
Saxi toxins151
Isoforms: Saxitoxin
(SIX), Neosaxitoxin
(NEOSTX), Gonyautoxin
(GTX),
Decarbamoylgonyautox
in (dcGTX),
Decarbamoylsaxitoxi n
lrtr*GT Y\
(dcSTA)
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Sample Size |21
50- 100 g
50- 100 g
50- 100 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 2811
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D2-4
June 2010
-------
Solid (Soil, Powder) — Small Molecule
Analyte(s)
T-2 Mycotoxin151
Tetrodotoxin151
Container
Sterile, sealed,
leakproof
container
Sterile, sealed,
leakproof
container
Preservation
Ship and store at
negative (-) 20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
50- 100 g
50- 100 g
Holding Time
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Minimize transport and
storage time. If feasible,
analyze or extract
immediately upon receipt
at the laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Aircraft: cargo only
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label'31
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D2-5
June 2010
-------
Particulate (Swabs, Wipes, Dust Socks) — Protein
Analyte(s)
Abrin'51
Botulinum
neuro toxins
(Serotoypes A, B, E,
F)(5)
Ricin'51
(Ricinine: ricin marker)
Shiga and Shiga-like
Toxins
(Stx, Stx-1,Stx-2)'51
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Ship and store at
negative (-) 20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group III
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
U.S. FDA, Bacteriological Analytical Manual
Online, Ch. 17,2001
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D3-1
June 2010
-------
Particulate (Swabs, Wipes, Dust Socks) — Protein
Analyte(s)
Staphylococcal
enterotoxins (SEE)'51
Staphylococcal
enterotoxins (SEA,
SEC)'51
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
993.06 (AOAC)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
993.06 (AOAC)
Particulate (Swabs, Wipes, Dust Sock) — Small Molecule
Analyte(s)
Aflatoxin(TypeBI)'51
a-Amanitin|51
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store in
the dark at
negative (-) 20°C.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group II
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D3-2
June 2010
-------
Particulate (Swabs, Wipes, Dust Sock) — Small Molecule
Analyte(s)
Anatoxin-a'5'
Brevetoxins
(B form)'51
a-Conotoxin'5'
Cylindrospermopsin151
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Ship and store at
negative (-) 20°C.
Ship and store at
negative (-) 20°C.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 2811
Label outer package with
the proper shipping name:
"Toxic solids, organic,
n.o.s." AND UN# 2811
This substance is
considered to be non-
hazardous for transport.
This substance is
considered to be non-
hazardous for transport.
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D3-3
June 2010
-------
Particulate (Swabs, Wipes, Dust Sock) — Small Molecule
Analyte(s)
Diacetoxyscirpenol
(DAS)(S|
Microcystin
Isoforms: LA, LR, YR,
LW, RR, YR(S|
Picrotoxin151
Saxi toxins151
Isoforms: Saxitoxin
(SIX), Neosaxitoxin
(NEOSTX),
Gonyautoxin (GTX),
Decarbamoylgonyauto
xin (dcGTX),
Decarbamoylsaxitoxi n
(dcSTX)
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Ship and store at
negative (-) 20°C.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 2811
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, liquid,
n.o.s." AND UN# 31 72
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D3-4
June 2010
-------
Particulate (Swabs, Wipes, Dust Sock) — Small Molecule
Analyte(s)
T-2 Mycotoxin151
Tetrodotoxin151
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Ship and store at
negative (-) 20°C.
Room
temperature if
held for 2 hours or
less; keep on ice
(e.g., ice packs,
secure double-
bagged ice) if
longer.
Sample Size |21
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
At least 2 sterile,
synthetic, and
moistened wipes or
swabs, or dust
socks
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Aircraft: cargo only
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7)
and (6).
Belongs to Packing Group 1
Shipping Label131
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Label outer package with
the proper shipping name:
"Toxins, extracted from
living sources, solid,
n.o.s." AND UN# 3462
Source/SAM Method'41
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
49 CFR 173.211 -213, Non-bulk Packaging
for Solid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for
Biological Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-
center.html (accessed September 12, 2009)
Sample Collection Information Document
D3-5
June 2010
-------
Liquid/Drinking Water181 — Protein
Analyte(s)
Abrin'51
Botulinum neurotoxins
(Serotoypes A, B, E, F)(s|
Ricin'51
(Ricinine: ricin marker)
Shiga and Shiga-like
Toxins
(Stx, Stx-1,Stx-2)(51
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Sample Size |21
A minimum of 100
mL
A minimum of 100
mL
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group III
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN33172
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN#3172
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
U.S. FDA, Bacteriological Analytical Manual
Online, Ch. 17
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D4-1
June 2010
-------
Liquid/Drinking Water181 — Protein
Analyte(s)
Staphylococcal
enterotoxins (SEE)'51
Staphylococcal
enterotoxins (SEA,
SEC)'51
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Sample Size |21
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Liciuid/Drinkinq Water181 — Small Molecule
Analyte(s)
Aflatoxin(TypeBI)'51
a-Amanitin'5'
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Ship and store at -20°C
in the dark.
Sample Size |21
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D4-2
June 2010
-------
Liquid/Drinking Water181 — Small Molecule
Analyte(s)
Anatoxin-a'5'
Brevetoxins
(B form)'51
a-Conotoxin'5'
Cylindrospermopsin151
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Ship and store at
negative (-) 20°C.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Ship and store at
negative (-) 20°C.
Sample Size |21
A minimum of 10
mL
A minimum of 100
mL
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnote (6).
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN# 281 1
Label outer package
with the proper
shipping name:
"Toxic solids,
organic, n.o.s." AND
UN# 281 1
This substance is
considered to be
non-hazardous for
transport.
This substance is
considered to be
non-hazardous for
transport.
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D4-3
June 2010
-------
Liquid/Drinking Water181 — Small Molecule
Analyte(s)
Diacetoxyscirpenol
(DAS)(S|
Microcystin
Isoforms: LA, LR, YR,
LW, RR, YR(S|
Picrotoxin151
Saxitoxins151
Isoforms: Saxitoxin
(SIX), Neosaxitoxin
(NEOSTX), Gonyautoxin
(GTX),
Decarbamoylgonyautoxi
n (dcGTX),
Decarbamoylsaxi toxin
(dcSTX)
Container
Sterile, sealed,
leakproof container
2.5 L glass bottles
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Ship on ice packs (or
secure double-bagged
ice); use immediately.
Ship and store in the
dark on ice packs (or
secure double-bagged
ice).
Ship and store at
negative (-) 20°C.
Sample Size |21
A minimum of 100
mL
2L
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group II
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 281 1
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
liquid, n.o.s." AND
UN#3172
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Sample Collection Information Document
D4-4
June 2010
-------
Liquid/Drinking Water181 — Small Molecule
Analyte(s)
T-2 Mycotoxin151
Tetrodotoxin151
Container
Sterile, sealed,
leakproof container
Sterile, sealed,
leakproof container
Preservation
Ship and store at
negative (-) 20°C.
Room temperature if
held for 2 hours or less;
keep on ice (e.g., ice
packs, secure double-
bagged ice) if longer.
Sample Size |21
A minimum of 100
mL
A minimum of 100
mL
Holding Time
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Minimize transport and
storage time. If
feasible, analyze or
extract immediately
upon receipt at the
laboratory.
Packaging
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Aircraft: cargo only
Decontaminate and label the
exterior of the container.
Place in a cooler and package
the samples outside the
contaminated area as
described in Footnotes (7) and
(6).
Belongs to Packing Group I
Shipping Label131
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Label outer package
with the proper
shipping name:
"Toxins, extracted
from living sources,
solid, n.o.s." AND
UN# 3462
Source/SAM Method'41
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
49 CFR 1 73.201 - 203, Non-bulk Packaging for
Liquid Hazardous Materials
USEPA, Draft Environmental Sampling
Guidelines and Analytical Approach for Biological
Response Plans, 2006
Sigma-Aldrich Chemical Co., St. Louis, MO,
Material Safety Data Sheet
http://www.sigmaaldrich.com/safety-center.html
(accessed September 12, 2009)
Footnotes:
(1) MCE and PTFE filters are also available as cassettes.
(2) The sample sizes listed are based on the amount needed for analysis of a single sample. If requested by the laboratory, additional sample(s) must be collected for laboratory qualitiy control analyses (e.g., duplicates,
matrix spikes). It is also recommended that additional sample(s) be collected in case of the need for reanalysis due to sample spillage or unforseen analytical difficulties.
(3) The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package
as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities
exception for Division 6.1 (poisonous) materials if: (1) The maximum quantity of material per inner receptacle or article is limited to—(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I,
Hazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Hazard Zone A or B
material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 -Definitions) do not require hazard labeling. For packages using dry
ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper
and recipient.
(4) SAM methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center (NHSRC), Standardized Analytical Methods for Environmental Restoration
Following Homeland Security Events, Revision 5.0, September 2009 (www.epa.gov/sam/).
(5) Currently, no information is available for this analyte in this sample type. Until such time that analye-specific information is available, collection procedures described for a similar analyte/sample type are considered to be
appropriate.
(6) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample
preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to
ensure sample temperatures do not exceed temperature requirements. Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the
sample containers. If dry ice is used, the outer packaging must be appropriately labeled (Footnote (3)).
(7) Hazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for
liquids, and at 173.211 - 173.213 for solids For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4.
(8) For collection of aqueous samples containing chlorine residual, add a stock solution of filter-sterilized 10% sodium thiosulfate at 0.5 mL/L.
Sample Collection Information Document
D4-5
June 2010
-------
United States
Environmental Protection
Agency
PRESORTED STANDARD
POSTAGE & FEES PAID
EPA
PERMIT NO. G-35
Office of Research and Development
National Homeland Security Research Center
Cincinnati, OH 45268
------- |