w Analytical Methods Approved for Compliance Monitoring under \ the Enhanced Surface Water Treatment Rule Analysis for the following contaminants shall be conducted in accordance with the methods in the following table or their equivalent as determined by EPA. ^ The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74. Additional methods are listed in Appendix A to Subpart ? C of Part 141. The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods information published in 40 CFR 141. If you find discrepancies, please notify The Safe Drinking Water Hotline (800-426-4791) so that EPA can correct the table. Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Disinfectants Free Chlorine 4500-Cl D Standard Methods 4500-Cl D Standard Methods 4500-Cl D Standard Methods 4500-Cl D Standard Methods 4500-Cl D-00 Standard Methods Online 4500-Cl F Standard Methods If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits. If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 1 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Disinfectants Free Chlorine 4500-Cl F Standard Methods 4500-Cl F Standard Methods 4500-Cl F Standard Methods 4500-Cl F-00 Standard Methods Online 4500-Cl G Standard Methods 4500-Cl G Standard Methods 4500-Cl G Standard Methods 4500-Cl G Standard Methods If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits. If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730. Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 2 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Disinfectants Free Chlorine 4500-Cl G-00 Standard Methods Online 4500-Cl H Standard Methods 4500-Cl H Standard Methods 4500-Cl H Standard Methods 4500-Cl H Standard Methods 4500-Cl H-00 Standard Methods Online If approved by the State, residual disinfectant concentrations f If approved by the State, free chlorine may be measured using Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3 Rock Hill, SC 29730. Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination 1 992 of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. D1253-03 ASTM Annual Book of ASTM Standards, Vol. International 11.01 D1253-86 ASTM Annual Book of ASTM Standards, Vol. International 11.01 ar free chlorine and combined chlorine may be measured using DPD colorimetric test kits. ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free .0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., http://www.standardmethods.org/ Standard Methods Standard Methods Standard Methods Standard Methods http://www.standardmethods.org/ http://www.astm.org http://www.astm.org Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 3 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Publication Order Date Number Number Source of Method Disinfectants Total Chlorine 4500-Cl D 4500-C1 D 4500-Cl D 4500-Cl D 4500-Cl D-00 4500-Cl E 4500-Cl E 4500-Cl E 4500-Cl E Standard Methods Standard Methods Standard Methods Standard Methods Standard Methods Online Standard Methods Standard Methods Standard Methods Standard Methods If approved by the State, residual disinfectant Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods for the Examination of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods for the Examination of Water and Wastewater, 21st Edition concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits. 1992 Standard Methods 1 995 Standard Methods 1 998 Standard Methods 2005 Standard Methods http://www.standardmethods.org/ 1 992 Standard Methods 1995 Standard Methods 1998 Standard Methods 2005 Standard Methods Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 4 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Publication Order Date Number Number Source of Method Disinfectants Total Chlorine 4500-Cl E-00 4500-C1 F 4500-Cl F 4500-Cl F 4500-Cl F 4500-Cl F-00 4500-Cl G 4500-Cl G 4500-Cl G Standard Methods Online Standard Methods Standard Methods Standard Methods Standard Methods Standard Methods Online Standard Methods Standard Methods Standard Methods If approved by the State, residual disinfectant Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods for the Examination of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods for the Examination of Water and Wastewater, 20th Edition concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits. http://www.standardmethods.org/ 1 992 Standard Methods 1995 Standard Methods 1 998 Standard Methods 2005 Standard Methods http://www.standardmethods.org/ 1992 Standard Methods 1 995 Standard Methods 1998 Standard Methods Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 5 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Publication Order Date Number Number Source of Method Disinfectants Total Chlorine 4500-Cl G Standard Methods 4500-Cl G-00 Standard Methods Online 4500-Cl I Standard Methods 4500-Cl I Standard Methods 4500-Cl I Standard Methods 4500-Cl I Standard Methods 4500-Cl 1-00 Standard Methods Online If approved by the State, residual disinfectant Standard Methods for the Examination of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods for the Examination of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. D1253-03 ASTM Annual Book of ASTM Standards, Vol. International 11.01 D1253-86 ASTM Annual Book of ASTM Standards, Vol. International 11.01 concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits. 2005 Standard Methods http://www.standardmethods.org/ 1992 Standard Methods 1 995 Standard Methods 1998 Standard Methods 2005 Standard Methods http://www.standardmethods.org/ http://www.astm.org http://www.astm.org Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 6 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Disinfectants Chlorine Dioxide 327 Rev 1.1 EPA 4500-C1O2 C Standard Methods 4500-C1O2 C Standard Methods 4500-C1O2 C Standard Methods 4500-C1O2 C Standard Methods 4500-C1O2 C-00 Standard Methods Online 4500-C1O2 D Standard Methods 4500-C1O2 D Standard Methods 4500-C1O2 D Standard Methods Determination of Chlorine Dioxide and May 2005 EPA 815-R-05-008 http://www.epa.gov/safewater/methods/sourcalt.html Chlorite Ion in Drinking Water Using Lissamine Green B and Horseradish Peroxidase with Detection by Visible Spectrophotometry Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 7 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Publication Order Number Number Source of Method Disinfectants Chlorine Dioxide 4500-C1O2 E Standard Methods 4500-C1O2 E Standard Methods 4500-C1O2 E Standard Methods 4500-C1O2 E Standard Methods 4500-C1O2 E-00 Standard Methods Online Ozone 4500-O3 B Standard Methods 4500-O3 B Standard Methods 4500-O3 B Standard Methods 4500-O3 B Standard Methods Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 8 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Disinfectants Ozone 4500-O3 B-97 Standard Methods Online Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. http://www.standardmethods.org/ Microbial Contaminants Total Coliforms 9221 A Standard Methods 9221 A Standard Methods 9221 A The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Standard Methods for the Examination Methods of Water and Wastewater, 20th Edition 1998 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 9 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms 9221 A Standard Methods 9221 A-99 Standard Methods Online 9221 B Standard Methods 9221 B The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. 2005 Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Standard Methods for the Examination Methods of Water and Wastewater, 19th Edition 1995 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 10 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. 9221 B Standard Methods 9221 B Standard Methods 9221 C Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. 9221 B-99 Standard Online version of Standard Methods for http://www.standardmethods.org/ Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Standard Methods for the Examination Methods of Water and Wastewater, 18th Edition 1992 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 11 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. 9221 C 9221 C 9221 C Standard Methods Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Standard Standard Methods for the Examination Methods of Water and Wastewater, 21 st Edition 2005 Standard Methods No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. 9221 C-99 Standard Online version of Standard Methods for http://www.standardmethods.org/ Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false- negative rate for total coliform, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two-thirds after the sample is added. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 12 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms 9222 A Standard Methods 9222 A Standard Methods 9222 A Standard Methods 9222 A The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Standard Methods for the Examination 1992 of Water and Wastewater, 18th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Standard Methods for the Examination Methods of Water and Wastewater, 21 st Edition 2005 Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 13 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms 9222 A-97 Standard Methods Online The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Online version of Standard Methods for http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. 9222 B Standard Standard Methods for the Examination Methods of Water and Wastewater, 18th Edition 9222 B Standard Methods 9222 B Standard Methods 1992 Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination 1995 of Water and Wastewater, 19th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination 1998 of Water and Wastewater, 20th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 14 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. 9222 B Standard Methods 9222 C Standard Methods 9222 C Standard Methods Standard Methods for the Examination 2005 of Water and Wastewater, 21st Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. 9222 B-97 Standard Online version of Standard Methods for http://www.standardmethods.org/ Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination 1992 of Water and Wastewater, 18th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination 1995 of Water and Wastewater, 19th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 15 of 20 ------- Contaminant Method Organization Reference Title EPA Publication Date Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms 9222 C 9222 C 9223 The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Standard Methods Standard Methods Standard Methods Standard Methods for the Examination 1998 of Water and Wastewater, 20th Edition Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. 9222 C-97 Standard Online version of Standard Methods for http://www.standardmethods.org/ Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required. Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen, IN 46527-0340. Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods The ONPG-MUG Test is also known as the Autoanalysis Colilert System. 9223 Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods The ONPG-MUG Test is also known as the Autoanalysis Colilert System. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 16 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Microbial Contaminants Total Coliforms The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. 9223 Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods The ONPG-MUG Test is also known as the Autoanalysis Colilert System. 9223 Standard Standard Methods for the Examination 2005 Methods of Water and Wastewater, 21 st Edition The ONPG-MUG Test is also known as the Autoanalysis Colilert System. Standard Methods 9223 B-97 Standard Online version of Standard Methods for Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. The ONPG-MUG Test is also known as the Autoanalysis Colilert System. http://www.standardmethods.org/ Fecal Coliforms 9221 E Standard Methods 9221 E Standard Methods 9221 E Standard Methods 9221 E Standard Methods The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Standard Methods for the Examination 1 992 Standard Methods of Water and Wastewater, 18th Edition A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C. Standard Methods for the Examination 1 995 Standard Methods of Water and Wastewater, 19th Edition A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C. Standard Methods for the Examination 1 998 Standard Methods of Water and Wastewater, 20th Edition A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C. Standard Methods for the Examination 2005 Standard Methods of Water and Wastewater, 21st Edition A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 17 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Publication Order Number Number Source of Method Microbial Contaminan Fecal Coliforms 9221 E-99 Standard Methods Online 9222 D Standard Methods 9222 D Standard Methods 9222 D Standard Methods 9222 D Standard Methods 9222 D-97 Standard Methods Online Heterotrophic Bacteria 9215 B Standard Methods 9215 B Standard Methods ts The time from sample collection to initiation of analysis may Online version of Standard Methods for not exceed 8 hours. Systems must hold samples below 10°C during transit. http://www.standardmethods.org/ the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C. Standard Methods for the Examination 1 992 of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. The time from sample collection to initiation of analysis may Standard Methods for the Examination 1 992 of Water and Wastewater, 18th Edition Standard Methods for the Examination 1 995 of Water and Wastewater, 19th Edition Standard Methods Standard Methods Standard Methods Standard Methods http://www.standardmethods.org/ not exceed 8 hours. Systems must hold samples below 10°C Standard Methods Standard Methods during transit. Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 18 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Microbial Contaminants Heterotrophic Bacteria 9215 B Standard Methods The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit. Standard Methods for the Examination 1998 Standard Methods of Water and Wastewater, 20th Edition 9215 B Standard Standard Methods for the Examination 2005 Methods of Water and Wastewater, 21 st Edition Standard Methods 9215B-00 Standard Online version of Standard Methods for Methods the Examination of Water and Online Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. http://www.standardmethods.org/ SimPlate® IDEXX Laboratories, Inc. IDEXX SimPlate TM HPC Test November Method for Heterotrophs in Water 2000 IDEXX Laboratories, Inc. Water Quality Parameters Turbidity Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g. Hach StablCal™ or equivalent) are acceptable substitutes for formazin. 10133 Rev. 2.0 Hach Co. Hach Filter Track Method, January 2000 "Determination of Turbidity by Laser Nephelometry," Revision 2.0 Hach Company 180.1 Rev 2.0 EPA In Methods for the Determination of August 1993 EPA/600/R-93/100 PB94-120821 http://www.nemi.gov Inorganic Substances in Environmental Samples 2130 B Standard Methods Standard Methods for the Examination 1992 of Water and Wastewater, 18th Edition Standard Methods Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 19 of 20 ------- Contaminant Method Organization Reference Title Date EPA Publication Number Publication Order Number Source of Method Water Quality Parameters Turbidity 2130B Standard Methods 21 SOB Standard Methods 21 SOB Standard Methods 2130B-01 Standard Methods Online Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g substitutes for formazin. Standard Methods for the Examination 1 995 of Water and Wastewater, 19th Edition Standard Methods for the Examination 1 998 of Water and Wastewater, 20th Edition Standard Methods for the Examination 2005 of Water and Wastewater, 21st Edition Online version of Standard Methods for the Examination of Water and Wastewater. Approval year by Standard Methods Committee is designated by last 2 digits. This is the only online version that is approved. Method 2 Great Lakes GLI Method 2, "Turbidity" November 2, Instruments, 1992 Inc. . Hach StablCal™ or equivalent) are acceptable Standard Methods Standard Methods Standard Methods http://www.standardmethods.org/ Great Lakes Instruments, Inc. Contact information for methods that are not available on the Internet are summarized in the report titled "Sources of Approved Analytical Methods for National Drinking Water Regulations." Revised June 2008 Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations Page 20 of 20 ------- |