w
Analytical Methods Approved for Compliance Monitoring under
\ the Enhanced Surface Water Treatment Rule
Analysis for the following contaminants shall be conducted in accordance with the methods in the following table or their equivalent as determined by EPA.
^ The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74. Additional methods are listed in Appendix A to Subpart
? C of Part 141.
The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods
information published in 40 CFR 141. If you find discrepancies, please notify The Safe Drinking Water Hotline (800-426-4791) so that EPA can correct the
table.
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D-00 Standard
Methods
Online
4500-Cl F Standard
Methods
If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
Rock Hill, SC 29730.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 1 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl F Standard
Methods
4500-Cl F Standard
Methods
4500-Cl F Standard
Methods
4500-Cl F-00 Standard
Methods
Online
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
Rock Hill, SC 29730.
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 2 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl G-00 Standard
Methods
Online
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H-00 Standard
Methods
Online
If approved by the State, residual disinfectant concentrations f
If approved by the State, free chlorine may be measured using
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3
Rock Hill, SC 29730.
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
D1253-03 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
D1253-86 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
ar free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
http://www.standardmethods.org/
Standard Methods
Standard Methods
Standard Methods
Standard Methods
http://www.standardmethods.org/
http://www.astm.org
http://www.astm.org
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 3 of 20
-------
Contaminant
Method
Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method
Disinfectants
Total Chlorine
4500-Cl D
4500-C1 D
4500-Cl D
4500-Cl D
4500-Cl D-00
4500-Cl E
4500-Cl E
4500-Cl E
4500-Cl E
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
If approved by the State, residual disinfectant
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
1992 Standard Methods
1 995 Standard Methods
1 998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/
1 992 Standard Methods
1995 Standard Methods
1998 Standard Methods
2005 Standard Methods
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 4 of 20
-------
Contaminant
Method
Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method
Disinfectants
Total Chlorine
4500-Cl E-00
4500-C1 F
4500-Cl F
4500-Cl F
4500-Cl F
4500-Cl F-00
4500-Cl G
4500-Cl G
4500-Cl G
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
If approved by the State, residual disinfectant
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
http://www.standardmethods.org/
1 992 Standard Methods
1995 Standard Methods
1 998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/
1992 Standard Methods
1 995 Standard Methods
1998 Standard Methods
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 5 of 20
-------
Contaminant
Method Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method
Disinfectants
Total Chlorine
4500-Cl G Standard
Methods
4500-Cl G-00 Standard
Methods
Online
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl 1-00 Standard
Methods
Online
If approved by the State, residual disinfectant
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
D1253-03 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
D1253-86 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
2005 Standard Methods
http://www.standardmethods.org/
1992 Standard Methods
1 995 Standard Methods
1998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/
http://www.astm.org
http://www.astm.org
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 6 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Chlorine Dioxide
327 Rev 1.1 EPA
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C-00 Standard
Methods
Online
4500-C1O2 D Standard
Methods
4500-C1O2 D Standard
Methods
4500-C1O2 D Standard
Methods
Determination of Chlorine Dioxide and May 2005 EPA 815-R-05-008 http://www.epa.gov/safewater/methods/sourcalt.html
Chlorite Ion in Drinking Water Using
Lissamine Green B and Horseradish
Peroxidase with Detection by Visible
Spectrophotometry
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 7 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA Publication
Publication Order
Number Number Source of Method
Disinfectants
Chlorine Dioxide
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E-00 Standard
Methods
Online
Ozone
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 8 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Ozone
4500-O3 B-97
Standard
Methods
Online
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
http://www.standardmethods.org/
Microbial Contaminants
Total Coliforms
9221 A
Standard
Methods
9221 A
Standard
Methods
9221 A
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 20th Edition
1998
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 9 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
9221 A
Standard
Methods
9221 A-99
Standard
Methods
Online
9221 B
Standard
Methods
9221 B
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
2005
Standard Methods
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 19th Edition
1995
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 10 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
9221 B
Standard
Methods
9221 B
Standard
Methods
9221 C
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
2005
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
9221 B-99 Standard Online version of Standard Methods for http://www.standardmethods.org/
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 18th Edition
1992
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 11 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
9221 C
9221 C
9221 C
Standard
Methods
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 21 st Edition
2005
Standard Methods
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
9221 C-99 Standard Online version of Standard Methods for http://www.standardmethods.org/
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
negative rate for total coliform, using lactose broth, is less than 10 percent.
Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 12 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
9222 A
Standard
Methods
9222 A
Standard
Methods
9222 A
Standard
Methods
9222 A
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination 1992
of Water and Wastewater, 18th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 21 st Edition
2005
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 13 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
9222 A-97
Standard
Methods
Online
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
9222 B
Standard Standard Methods for the Examination
Methods of Water and Wastewater, 18th Edition
9222 B
Standard
Methods
9222 B
Standard
Methods
1992
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination 1995
of Water and Wastewater, 19th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination 1998
of Water and Wastewater, 20th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 14 of 20
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Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
9222 B
Standard
Methods
9222 C
Standard
Methods
9222 C
Standard
Methods
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
9222 B-97 Standard Online version of Standard Methods for http://www.standardmethods.org/
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination 1992
of Water and Wastewater, 18th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination 1995
of Water and Wastewater, 19th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 15 of 20
-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
9222 C
9222 C
9223
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard
Methods
Standard
Methods
Standard
Methods
Standard Methods for the Examination 1998
of Water and Wastewater, 20th Edition
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
2005
Standard Methods
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
9222 C-97 Standard Online version of Standard Methods for http://www.standardmethods.org/
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
Standard Methods
The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
9223
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 16 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Total Coliforms
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
9223
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
9223
Standard Standard Methods for the Examination 2005
Methods of Water and Wastewater, 21 st Edition
The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
Standard Methods
9223 B-97 Standard Online version of Standard Methods for
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
http://www.standardmethods.org/
Fecal Coliforms
9221 E Standard
Methods
9221 E Standard
Methods
9221 E Standard
Methods
9221 E Standard
Methods
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 17 of 20
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Contaminant
Method Organization Reference Title Date
EPA Publication
Publication Order
Number Number Source of Method
Microbial Contaminan
Fecal Coliforms
9221 E-99 Standard
Methods
Online
9222 D Standard
Methods
9222 D Standard
Methods
9222 D Standard
Methods
9222 D Standard
Methods
9222 D-97 Standard
Methods
Online
Heterotrophic Bacteria
9215 B Standard
Methods
9215 B Standard
Methods
ts
The time from sample collection to initiation of analysis may
Online version of Standard Methods for
not exceed 8 hours. Systems must hold samples below 10°C during transit.
http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
The time from sample collection to initiation of analysis may
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods
Standard Methods
Standard Methods
Standard Methods
http://www.standardmethods.org/
not exceed 8 hours. Systems must hold samples below 10°C
Standard Methods
Standard Methods
during transit.
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 18 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Microbial Contaminants
Heterotrophic Bacteria
9215 B
Standard
Methods
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination 1998 Standard Methods
of Water and Wastewater, 20th Edition
9215 B
Standard Standard Methods for the Examination 2005
Methods of Water and Wastewater, 21 st Edition
Standard Methods
9215B-00 Standard Online version of Standard Methods for
Methods the Examination of Water and
Online Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
http://www.standardmethods.org/
SimPlate®
IDEXX
Laboratories,
Inc.
IDEXX SimPlate TM HPC Test November
Method for Heterotrophs in Water 2000
IDEXX Laboratories, Inc.
Water Quality Parameters
Turbidity
Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g. Hach StablCal™ or equivalent) are acceptable
substitutes for formazin.
10133 Rev. 2.0 Hach Co.
Hach Filter Track Method, January 2000
"Determination of Turbidity by Laser
Nephelometry," Revision 2.0
Hach Company
180.1 Rev 2.0 EPA
In Methods for the Determination of August 1993 EPA/600/R-93/100 PB94-120821 http://www.nemi.gov
Inorganic Substances in Environmental
Samples
2130 B
Standard
Methods
Standard Methods for the Examination 1992
of Water and Wastewater, 18th Edition
Standard Methods
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 19 of 20
-------
Contaminant
Method Organization Reference Title
Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Water Quality Parameters
Turbidity
2130B Standard
Methods
21 SOB Standard
Methods
21 SOB Standard
Methods
2130B-01 Standard
Methods
Online
Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g
substitutes for formazin.
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Method 2 Great Lakes GLI Method 2, "Turbidity" November 2,
Instruments, 1992
Inc.
. Hach StablCal™ or equivalent) are acceptable
Standard Methods
Standard Methods
Standard Methods
http://www.standardmethods.org/
Great Lakes Instruments, Inc.
Contact information for methods that are not available on the Internet are summarized in the report titled
"Sources of Approved Analytical Methods for National Drinking Water Regulations."
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 20 of 20
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