w
        Analytical Methods Approved for Compliance Monitoring under

\      the Enhanced  Surface Water Treatment Rule
        Analysis for the following contaminants shall be conducted in accordance with the methods in the following table or their equivalent as determined by EPA.
^      The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74. Additional methods are listed in Appendix A to Subpart
?       C of Part 141.

        The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods
        information published in 40 CFR 141. If you find discrepancies, please notify The Safe Drinking Water Hotline (800-426-4791) so that EPA can correct the
        table.
   Contaminant

     Method     Organization   Reference Title
                                       Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D Standard
Methods
4500-Cl D-00 Standard
Methods
Online
4500-Cl F Standard
Methods
If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
Rock Hill, SC 29730.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Revised June 2008
              Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                  Page 1 of 20

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   Contaminant

     Method      Organization   Reference Title
                            Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl F Standard
Methods
4500-Cl F Standard
Methods
4500-Cl F Standard
Methods
4500-Cl F-00 Standard
Methods
Online
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
4500-Cl G Standard
Methods
If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
If approved by the State, free chlorine may be measured using ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
Rock Hill, SC 29730.
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                       Page 2 of 20

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   Contaminant

     Method      Organization   Reference Title
                            Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Free Chlorine
4500-Cl G-00 Standard
Methods
Online
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H Standard
Methods
4500-Cl H-00 Standard
Methods
Online
If approved by the State, residual disinfectant concentrations f
If approved by the State, free chlorine may be measured using
Chlorine Species (HOC1- and OC1-) by Test Strip," Revision 3
Rock Hill, SC 29730.
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
D1253-03 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
D1253-86 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
ar free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
ITS free chlorine test strips. Use of the test strips is described in Method D99-003, "Free
.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St.,
http://www.standardmethods.org/
Standard Methods
Standard Methods
Standard Methods
Standard Methods
http://www.standardmethods.org/

http://www.astm.org
http://www.astm.org
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                       Page 3 of 20

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Contaminant
Method
Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method

Disinfectants

Total Chlorine
4500-Cl D
4500-C1 D
4500-Cl D
4500-Cl D
4500-Cl D-00
4500-Cl E
4500-Cl E
4500-Cl E
4500-Cl E
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
If approved by the State, residual disinfectant
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
1992 Standard Methods
1 995 Standard Methods
1 998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/
1 992 Standard Methods
1995 Standard Methods
1998 Standard Methods
2005 Standard Methods
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 4 of 20

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Contaminant
Method
Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method

Disinfectants

Total Chlorine
4500-Cl E-00
4500-C1 F
4500-Cl F
4500-Cl F
4500-Cl F
4500-Cl F-00
4500-Cl G
4500-Cl G
4500-Cl G
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Standard
Methods
Online
Standard
Methods
Standard
Methods
Standard
Methods
If approved by the State, residual disinfectant
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
http://www.standardmethods.org/

1 992 Standard Methods
1995 Standard Methods
1 998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/
1992 Standard Methods
1 995 Standard Methods
1998 Standard Methods
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 5 of 20

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Contaminant
Method Organization Reference Title
EPA Publication
Publication Order
Date Number Number Source of Method

Disinfectants
Total Chlorine
4500-Cl G Standard
Methods
4500-Cl G-00 Standard
Methods
Online
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl I Standard
Methods
4500-Cl 1-00 Standard
Methods
Online
If approved by the State, residual disinfectant
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
D1253-03 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
D1253-86 ASTM Annual Book of ASTM Standards, Vol.
International 11.01
concentrations for free chlorine and combined chlorine may be measured using DPD colorimetric test kits.
2005 Standard Methods
http://www.standardmethods.org/
1992 Standard Methods
1 995 Standard Methods
1998 Standard Methods
2005 Standard Methods
http://www.standardmethods.org/

http://www.astm.org

http://www.astm.org
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 6 of 20

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   Contaminant

     Method      Organization   Reference Title
                            Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Disinfectants
Chlorine Dioxide
327 Rev 1.1 EPA
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C Standard
Methods
4500-C1O2 C-00 Standard
Methods
Online
4500-C1O2 D Standard
Methods
4500-C1O2 D Standard
Methods
4500-C1O2 D Standard
Methods
Determination of Chlorine Dioxide and May 2005 EPA 815-R-05-008 http://www.epa.gov/safewater/methods/sourcalt.html
Chlorite Ion in Drinking Water Using
Lissamine Green B and Horseradish
Peroxidase with Detection by Visible
Spectrophotometry
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                       Page 7 of 20

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   Contaminant

     Method      Organization   Reference Title
                            Date
EPA            Publication
Publication      Order
Number         Number      Source of Method
Disinfectants
Chlorine Dioxide
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E Standard
Methods
4500-C1O2 E-00 Standard
Methods
Online

Ozone
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
4500-O3 B Standard
Methods
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Online version of Standard Methods for http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                       Page 8 of 20

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    Contaminant
      Method        Organization    Reference Title
                                                       Date
                                                  EPA
                                                  Publication
                                                  Number
                                Publication
                                Order
                                Number
 Source of Method
Disinfectants
   Ozone
     4500-O3 B-97
Standard
Methods
Online
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits.  This is the only online
version that is approved.
                                                http://www.standardmethods.org/
Microbial Contaminants
   Total Coliforms
     9221 A
Standard
Methods
     9221 A
Standard
Methods
     9221 A
                                     The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
Standard Methods
                                              No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                              Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                              this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                              negative rate for total coliform, using lactose broth, is less than 10 percent.
                                              Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
                                              No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                              Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                              this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                              negative rate for total coliform, using lactose broth, is less than 10 percent.
                                              Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard          Standard Methods for the Examination
Methods          of Water and Wastewater, 20th Edition
                                     1998
                                                Standard Methods
                                              No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                              Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                              this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                              negative rate for total coliform, using lactose broth, is less than 10 percent.
                                              Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
                     Approved Methods for the Surface Water Treatment Rule -  National Primary Drinking Water Regulations
                                                                                                                     Page 9 of 20

-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
     9221 A
Standard
Methods
     9221 A-99
Standard
Methods
Online
     9221 B
Standard
Methods
     9221 B
                                       The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
                                      2005
                                                 Standard Methods
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
      No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
      Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
      this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
      negative rate for total coliform, using lactose broth, is less than 10 percent.
      Media should cover inverted tubes at least one-half to two-thirds after the sample is added.

Online version of Standard Methods for                                                     http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
      No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
      Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
      this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
      negative rate for total coliform, using lactose broth, is less than 10 percent.
      Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
                                                                                        Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard           Standard Methods for the Examination
Methods           of Water and Wastewater, 19th Edition
                                       1995
                                                 Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                        Page 10 of 20

-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
                                       The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
     9221 B
Standard
Methods
     9221 B
Standard
Methods
     9221 C
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
2005
Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.

     9221 B-99        Standard           Online version of Standard Methods for                                                    http://www.standardmethods.org/
                       Methods           the Examination of Water and
                       Online             Wastewater. Approval year by Standard
                                          Methods Committee is designated by
                                          last 2 digits.  This is the only online
                                          version that is approved.
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard           Standard Methods for the Examination
Methods           of Water and Wastewater, 18th Edition
                                       1992
                                                  Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                        Page 11 of 20

-------
Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
                                       The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
     9221 C
     9221 C
     9221 C
Standard
Methods
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Standard           Standard Methods for the Examination
Methods           of Water and Wastewater, 21 st Edition
                                      2005
                                                 Standard Methods
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.

     9221 C-99        Standard           Online version of Standard Methods for                                                    http://www.standardmethods.org/
                       Methods           the Examination of Water and
                       Online             Wastewater. Approval year by Standard
                                          Methods Committee is designated by
                                          last 2 digits.  This is the only online
                                          version that is approved.
                                               No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes.
                                               Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between
                                               this medium and lauryl tryptose broth using the water normally tested, and this comparison demonstrates that the false-positive rate and false-
                                               negative rate for total coliform, using lactose broth, is less than 10 percent.
                                               Media should cover inverted tubes at least one-half to two-thirds after the sample is added.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                        Page 12 of 20

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Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
     9222 A
Standard
Methods
     9222 A
Standard
Methods
     9222 A
Standard
Methods
     9222 A
                                      The time from sample collection to initiation of analysis may not exceed 8 hours.  Systems must hold samples below 10°C during transit.
Standard Methods for the Examination    1992
of Water and Wastewater, 18th Edition
                                                Standard Methods
                                              MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at:  http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
                                              MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at:  http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
                                              MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at:  http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Standard          Standard Methods for the Examination
Methods          of Water and Wastewater, 21 st Edition
                                      2005
                                                Standard Methods
                                              MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at:  http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                      Page 13 of 20

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Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
     9222 A-97
Standard
Methods
Online
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.


   Online version of Standard Methods for                                                   http://www.standardmethods.org/
   the Examination of Water and
   Wastewater. Approval year by Standard
   Methods Committee is designated by
   last 2 digits.  This is the only online
   version that is approved.
         MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
         coliform andEscherichia coli in water" by Brenner, K.P., etal,  1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
         at:  http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
         Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
         IN 46527-0340.
     9222 B
Standard          Standard Methods for the Examination
Methods          of Water and Wastewater, 18th Edition
     9222 B
Standard
Methods
     9222 B
Standard
Methods
                                         1992
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at: http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
   Standard Methods for the Examination    1995
   of Water and Wastewater, 19th Edition
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at: http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
   Standard Methods for the Examination    1998
   of Water and Wastewater, 20th Edition
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at: http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                         Page 14 of 20

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Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
                                      The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
     9222 B
Standard
Methods
     9222 C
Standard
Methods
     9222 C
Standard
Methods
Standard Methods for the Examination    2005
of Water and Wastewater, 21st Edition
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at:  http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.

     9222 B-97         Standard           Online version of Standard Methods for                                                    http://www.standardmethods.org/
                       Methods           the Examination of Water and
                       Online             Wastewater. Approval year by Standard
                                         Methods Committee is designated by
                                         last 2 digits. This is the only online
                                         version that is approved.
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at:  http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
Standard Methods for the Examination    1992
of Water and Wastewater, 18th Edition
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at:  http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
Standard Methods for the Examination    1995
of Water and Wastewater, 19th Edition
Standard Methods
                                               MI agar also may be used.  Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                               coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                               at:  http://www.epa.gov/nerlcwww/online.htm.  Verification of colonies is not required.
                                               Coliscan® is approved as a modification of MI under the ATP program. It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                               IN 46527-0340.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                      Page 15 of 20

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Contaminant
Method Organization Reference Title
EPA
Publication
Date Number
Publication
Order
Number
Source of Method

Microbial Contaminants
   Total Coliforms
     9222 C
     9222 C
     9223
                                      The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard
Methods
Standard
Methods
Standard
Methods
Standard Methods for the Examination    1998
of Water and Wastewater, 20th Edition
                                                Standard Methods
                                              MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform andEscherichia coli in water" by Brenner, K.P., etal, 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 21st Edition
2005
Standard Methods
                                              MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.

     9222 C-97         Standard          Online version of Standard Methods for                                                   http://www.standardmethods.org/
                       Methods          the Examination of Water and
                       Online            Wastewater. Approval year by Standard
                                        Methods Committee is designated by
                                        last 2 digits. This is the only online
                                        version that is approved.
                                              MI agar also may be used. Preparation and use of MI agar is set forth in the article, "New medium for the simultaneous detection of total
                                              coliform and Escherichia coli in water" by Brenner, K.P., etal., 1993, Appl. Environ. Microbiol. 59:3534-3544. EPA/600/J-99/225. Available
                                              at: http://www.epa.gov/nerlcwww/online.htm. Verification of colonies is not required.
                                              Coliscan® is approved as a modification of MI under the ATP program.  It is available from Micrology Laboratories, P.O. Box 340, Goshen,
                                              IN 46527-0340.
Standard Methods for the Examination
of Water and Wastewater, 18th Edition
1992
Standard Methods
                                              The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
     9223
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 19th Edition
1995
Standard Methods
                                              The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
Revised June 2008
                      Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                                     Page 16 of 20

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   Contaminant
      Method       Organization    Reference Title
                                                   Date
                                              EPA
                                              Publication
                                              Number
                              Publication
                              Order
                              Number
 Source of Method
Microbial Contaminants
   Total Coliforms
                                   The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
     9223
Standard
Methods
Standard Methods for the Examination
of Water and Wastewater, 20th Edition
1998
Standard Methods
                                           The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
     9223
Standard          Standard Methods for the Examination    2005
Methods          of Water and Wastewater, 21 st Edition
                      The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
                                                                               Standard Methods
     9223 B-97        Standard         Online version of Standard Methods for
                     Methods         the Examination of Water and
                     Online           Wastewater. Approval year by Standard
                                     Methods Committee is designated by
                                     last 2 digits. This is the only online
                                     version that is approved.
                                           The ONPG-MUG Test is also known as the Autoanalysis Colilert System.
                                                                                               http://www.standardmethods.org/
Fecal Coliforms
9221 E Standard
Methods
9221 E Standard
Methods
9221 E Standard
Methods
9221 E Standard
Methods
The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10°C during transit.
Standard Methods for the Examination 1 992 Standard Methods
of Water and Wastewater, 18th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 995 Standard Methods
of Water and Wastewater, 19th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 998 Standard Methods
of Water and Wastewater, 20th Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 2005 Standard Methods
of Water and Wastewater, 21st Edition
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Revised June 2008
                    Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                           Page 17 of 20

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Contaminant
Method Organization Reference Title Date
EPA Publication
Publication Order
Number Number Source of Method

Microbial Contaminan
Fecal Coliforms
9221 E-99 Standard
Methods
Online
9222 D Standard
Methods
9222 D Standard
Methods
9222 D Standard
Methods
9222 D Standard
Methods
9222 D-97 Standard
Methods
Online

Heterotrophic Bacteria
9215 B Standard
Methods
9215 B Standard
Methods
ts
The time from sample collection to initiation of analysis may
Online version of Standard Methods for
not exceed 8 hours. Systems must hold samples below 10°C during transit.
http://www.standardmethods.org/
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C.
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
The time from sample collection to initiation of analysis may
Standard Methods for the Examination 1 992
of Water and Wastewater, 18th Edition
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods
Standard Methods
Standard Methods
Standard Methods




http://www.standardmethods.org/
not exceed 8 hours. Systems must hold samples below 10°C
Standard Methods
Standard Methods
during transit.

Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
Page 18 of 20

-------
    Contaminant
      Method       Organization   Reference Title
                                                    Date
                                                  EPA
                                                  Publication
                                                  Number
Publication
Order
Number
Source of Method
Microbial Contaminants
   Heterotrophic Bacteria
     9215 B
Standard
Methods
The time from sample collection to initiation of analysis may not exceed 8 hours.  Systems must hold samples below 10°C during transit.


   Standard Methods for the Examination    1998                                         Standard Methods
   of Water and Wastewater, 20th Edition
     9215 B
Standard          Standard Methods for the Examination    2005
Methods          of Water and Wastewater, 21 st Edition
                                                                                  Standard Methods
     9215B-00        Standard          Online version of Standard Methods for
                     Methods          the Examination of Water and
                     Online            Wastewater. Approval year by Standard
                                      Methods Committee is designated by
                                      last 2 digits. This is the only online
                                      version that is approved.
                                                                                                http://www.standardmethods.org/
     SimPlate®
IDEXX
Laboratories,
Inc.
   IDEXX SimPlate TM HPC Test        November
   Method for Heterotrophs in Water       2000
               IDEXX Laboratories, Inc.
 Water Quality Parameters
   Turbidity
              Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g. Hach StablCal™ or equivalent) are acceptable
              substitutes for formazin.
     10133 Rev. 2.0    Hach Co.
                 Hach Filter Track Method,              January 2000
                 "Determination of Turbidity by Laser
                 Nephelometry," Revision 2.0
                                                                                  Hach Company
     180.1 Rev 2.0     EPA
                 In Methods for the Determination of     August 1993  EPA/600/R-93/100   PB94-120821   http://www.nemi.gov
                 Inorganic Substances in Environmental
                 Samples
     2130 B
Standard
Methods
   Standard Methods for the Examination    1992
   of Water and Wastewater, 18th Edition
               Standard Methods
Revised June 2008
                    Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                                                                                               Page 19 of 20

-------
   Contaminant

    Method     Organization  Reference Title
                        Date
EPA
Publication
Number
Publication
Order
Number
Source of Method
Water Quality Parameters
Turbidity
2130B Standard
Methods
21 SOB Standard
Methods
21 SOB Standard
Methods
2130B-01 Standard
Methods
Online
Styrene divinyl benzene beads (e.g. AMCO-AEPA-1 or equivalent) and stabilized formazin (e.g
substitutes for formazin.
Standard Methods for the Examination 1 995
of Water and Wastewater, 19th Edition
Standard Methods for the Examination 1 998
of Water and Wastewater, 20th Edition
Standard Methods for the Examination 2005
of Water and Wastewater, 21st Edition
Online version of Standard Methods for
the Examination of Water and
Wastewater. Approval year by Standard
Methods Committee is designated by
last 2 digits. This is the only online
version that is approved.
Method 2 Great Lakes GLI Method 2, "Turbidity" November 2,
Instruments, 1992
Inc.
. Hach StablCal™ or equivalent) are acceptable
Standard Methods
Standard Methods
Standard Methods
http://www.standardmethods.org/
Great Lakes Instruments, Inc.
 Contact information for methods that are not available on the Internet are summarized in the report titled
 "Sources of Approved Analytical Methods for National Drinking Water Regulations."
Revised June 2008
Approved Methods for the Surface Water Treatment Rule - National Primary Drinking Water Regulations
                                               Page 20 of 20

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