&EPA
United States
Environmental Protection
Agency
Office of Water
4305T
Washington, DC 20460
EPA-823-R-02-005
Quality Assurance Project Plan for
Sample Collection Activities for a
National Study of Chemical Residues in
Lake Fish Tissue
Prepared/or:
Leanne Stahl, National Study Manager
U.S. Environmental Protection Agency, Office of Science and Technology
1200 Pennsylvania Avenue, NW (MC 4305T)
Washington, D.C. 20460
Prepared by:
Tetra Tech, Inc.
10045 Red Run Boulevard, Suite 110
Owings Mills, MD 21117
May 2000
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Quality Assurance Project Plan
for Sample Collection Activities for a
National Study of Chemical Residues in Lake Fish Tissue
Prepared/or:
Leanne Stahl, Work Assignment Manager
U.S. Environmental Protection Agency, Office of Science and Technology
1200 Pennsylvania Avenue, NW (MC 4305)
Washington, D.C. 20460
Prepared by:
Tetra Tech, Inc.
10045 Red Run Boulevard, Suite 110
Owings Mills, MD 21117
May 22, 2000
Final
This quality assurance project plan (QAPP) has been prepared according to guidance
provided in the document EPA Requirements for Quality Assurance Project Plans (EPA
QA/R-5, U.S. Environmental Protection Agency, Quality Assurance Division,
Washington, DC, Interim Final, November 1999) to ensure that environmental and
related data collected, compiled, and/or generated for this project are complete,
accurate, and of the type, quantity, and quality required for their intended use. The
work conducted by Tetra Tech will be in conformance with the quality assurance
program described in the quality management plan for Tetra Tech's Fairfax Group and
with the procedures detailed in this QAPP.
Approvals:
Esther C. Peters, Ph.D.
Quality Assurance Officer
Tetra Tech, Inc.
Date
Elaine D. Snyder !Y
Work Assignment Leader
Tetra Tech, Inc.
'Date
William Telliard
Quality Assurance Manager
USEPA, Office of Science &
Technology
Leanne Stahl
Work Assignment Manager
USEPA, Office of Science &
Technology
Date
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page iii of xv
CONTENTS
List of Tables v
List of Figures v
Distribution List vi
A Project Management
1.0 Project/Task Organization 1
2.0 Problem Definition/Background 4
3.0 Project/Task Description 6
4.0 Quality Objectives and Criteria for Measurement Data 9
4.1 Project Quality Objectives 9
4.2 Measurement Performance Criteria 10
5.0 Special Training Requirements/Certification 13
6.0 Documentation and Records 13
B Data Acquisition
7.0 Sampling Process Design 16
7.1 Sample Type 16
7.2 Sampling Period 17
7.3 Sample Frame 17
7.4 Selection of Lakes for Sampling 18
7.5 Nontarget Population, Inaccessible Lakes, and Lakes for Which
Access is Denied 19
7.6 Reserve Sample of Lakes 20
8.0 Sampling Methods 21
8.1 Target Species 21
8.2 Composite Sampling 22
8.3 Sample Collection 23
9.0 Sample Handling and Custody Requirements 25
9.1 Sample Handling 25
9.2 Sample Integrity 26
9.3 Custody Requirements 27
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
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10.0 Analytical Methods Requirements 30
11.0 Quality Control Requirements 30
12.0 Instrument/Equipment Testing, Inspection, and Maintenance Requirements . 30
13.0 Instrument Calibration and Frequency 31
14.0 Inspection/Acceptance Requirements for Supplies and Consumables 31
15.0 Data Acquisition Requirements (Nondirect Measurements) 31
16.0 Data Management 31
C Assessment/Oversight
17.0 Assessment and Response Actions 32
18.0 Reports to Management 34
D Data Validation and Usability
19.0 Data Review, Validation, and Verification Requirements 34
20.0 Validation and Verification Methods 34
21.0 Reconciliation with Data Quality Objectives 35
Literature Cited 36
Appendices
A Randomly Selected List of Target Lakes
B Standard Operating Procedure: Fish Tissue Sample Collection Procedures for a National Study
of Chemical Residues in Lake Fish Tissue
C Standard Operating Procedure: pH Measurements
D Field Data Element Dictionary
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
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Page v of xv
LIST OF TABLES
Table 1 Project Time Line for Milestones Associated with Fish Tissue Sample
Collection Activities 8
Table 2 Types of Field Data to Be Collected in Association with Fish Tissue Sample
Collection 10
Table 3 Numbers of Lakes by Size Category in Sample Frame (Based on RF3) 18
Table 4 Number of Lakes Selected for Sampling by Size Category and Year 19
Table 5 Number of Lakes (by Size Category and Year) Selected as a Reserve Sample .... 20
Table 6 Recommended Target Species for Inland Freshwaters (in Order of
Preference) 22
Table 7 Equipment and Supply List for Fish Tissue Sampling 24
LIST OF FIGURES
Figure 1 Organizational Diagram for the National Study of Chemical Residues
in Lake Fish Tissue 2
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page vi of xv
DISTRIBUTION LIST
James Amrhein
WI Department of Natural Resources
P.O. Box 7921
Madison, WI 53707
608/266-5325 (phone)
608/266-2244 (fax)
Tom Armitage
USEPA/OST (4305)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-5388 (phone)
202/260-9830 (fax)
Alan Auwarter
EPA Region 4
Science and Ecosystem Support Division
980 College Station Road
Athens, GA 30605-2720
706/355-8704 (phone)
706/355-8726 (fax)
John Biagi
GADNR
Wildlife Resources Division
The Stream Team
2425 Marbin Farms Road
Mansfield, GA 30055
770/784-3126 (phone)
770-784-3129 (fax)
William Botts
PADEP
909 Elmerton Avenue
Harrisburg,PA17110
717/705-4776 (phone)
717/705-4760 (fax)
Mark A. Briggs
MN Department of Natural Resources
500 Lafayette Road, Box 25
St. Paul, MN 55155-4025
651/215-0316 (phone)
651/296-1811 (fax)
Robert Brodberg
CA Environmental Protection Agency
Office of Environmental Health Hazard
Assessment
301 Capitol Mall, Rm 205
Sacramento, CA 95814-4327
916/323-4763 (phone)
916/327-7320 (fax)
Alan Buchanan
Missouri Department of Conservation
1110 S. College Ave.
Columbia, MO 65201
573/882-9880 X3257 (phone)
573/882-4517 (fax)
Lawrence Burkhard
USEPA Environmental Effects Research
Lab
Mid Continent Ecology Division/ORD
6201 Congdon Boulevard
Duluth, MN 55804
218/529-5164 (phone)
218/529-5003 (fax)
Dan Butler
OKCC
413 Northwest 12th Street
Oklahoma City, OK 73103-3706
405/979-2206 (phone)
405/979-2212 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Page vii of xv
Michael Callam
NE Department of Environmental Quality
1200 N Street, Suite 400
Atrium Bldg.
Lincoln, NE 68509-8922
402/471-4249 (phone)
402/471-2909 (fax)
Bud Cann
NJDEP
P.O. Box 427, 35 Arctic Parkway
Trenton, NJ 08625
609/292-0427 (phone)
609/633-1095 (fax)
Robert W. Cooner
AL Dept. of Environmental Management
P.O. Box 301463
Montgomery, AL 36130-1463
334/260-2746 (phone)
334/272-8131 (fax)
Sarah Conrad
DynCorp, Inc.
6101 Stevenson Avenue
Alexandria, VA 22304
703/461-2373 (phone)
Dave Courtemanch
MEDEP
State House #17
Augusta, ME 04333
207/287-7789 (phone)
207/287-7191 (fax)
Dave Crane
CA Department of Fish and Game
Water Pollution Control Lab
2005 Nimbus Road
Rancho Cordova, CA 95670
916/358-2859 (phone)
916/985-4301(fax)
Steve Cringan
KS Department of Health & Environment
Division of Environment
Forbes Field
Topeka, KS 66620
785/296-5571 (phone)
Phil Crocker
USEPA, Region 6
Fountain Place/6WQ-EW
1445 Ross Avenue
Suite 1200
Dallas, TX 75202-2733
214/665-6644 (phone)
214/665-6689 (fax)
Marc Dahlberg
Arizona Game and Fish Department
2221 W. Greenway
Phoenix, AZ 85023
602/789-3260 (phone)
602/789-3265 (fax)
Bob Day
MI Department of Environmental Quality
Surface Water Quality Division
Knapps Centre
P.O. Box 30273
Lansing, MI 48909-7773
517/335-3314 (phone)
517/373-9958 (fax)
Richard Denton
UT Department of Environmental Quality
Division of Water Quality
P.O. Box 144870
Salt Lake City, UT 84114-4870
801/538-6055 (phone)
801/538-6016 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Page viii of xv
Kevin Dorman
AR Department of Environmental Quality
P.O. Box 8912
Little Rock, AR 72219-8912
501/682-0666 (phone)
501/682-0910 (fax)
Don Dycus
Tennessee Valley Authority
1101 Market Street, CST17D
Chattanooga, TN 37402
423/751-7322 (phone)
423/751-7648 (fax)
Robert Estabrook
NHDES
6 Hazen Drive
Concord, NH 03301
603/271-3357 (phone)
603/271-7899 (fax)
Mike Ell
ND Department of Health
Division of Water Quality
1200 Missouri Ave., Rm 203
Bismarck, ND 58506
701/328-5214 (phone)
701/328-5200 (fax)
John Ford
MO Department of Natural Resources
P.O. Box 176
Jefferson City, MO 65102
573/751-7428 (phone)
573/751-7024 (fax)
Gene Foster
OR Department of Environmental Quality
Water Quality Division
811 SW 6th Avenue
Portland, OR 97204
503/229-5358 (phone)
503/229-6124 (fax)
Robert Frey
PA Department of Environmental Protection
Bureau of Watershed Conservation
P.O. Box 8555
Harrisburg, PA 17105-8555
7177787-9637 (phone)
717/787-9549 (fax)
Mike George
UtahDEQ
288 N, 1460 W
Salt Lake City, UT 84114-4870
801/538-9325 (phone)
801/538-6016 (fax)
Al Gibson
MS DEQ, Field Services Division
Biological Services Section
1542 Old Whirfield Road
Pearl, MS 39208
601/664-3963 (phone)
601/664-3938 (fax)
Jim Green
US EPA Wheeling Field Office
1060 Chapline Street, Suite 303
Wheeling, WV 26003-2995
304/234-0243 (phone)
304/234-0260 (fax)
Jean Gregory
VA Department of Environmental Quality
P.O. Box 10009
Richmond, VA 23240-0009
804/698-4113 (phone)
Mark Hale
NC DENR, Division of Water Quality
Ecosystems Analysis Unit
4401 Reedy Creek Road
Raleigh, NC 27607
919/733-6946 x240 (phone)
919/733-9959 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Page ix of xv
Robert Hanten
SD Game, Fish, and Parks
523 East Capitol Street
Pierre, SD 57501
605/223-7702 (phone)
605/773-3399 (fax)
Scott Hardin
FL Game & Freshwater Fish Commission
Farris Bryant Building
620 S. Meridian Street
Tallahassee, FL 32399-1600
850/488-4068 (phone)
Chris Harman
AL DEM, Montgomery Branch
Field Operations Division
1890 Dickinson Drive, Building A
Montgomery, AL 36109
334/260-2751 (phone)
Linda Harn
GA Department of Natural Resources
Environmental Protection Division
Watershed Planning & Monitoring Program
7 Martin Luther King Jr. Drive, Suite 643
Atlanta, GA 30330
404/656-4905 (phone)
404/657-7031 (fax)
Janet Hashimoto
USEPA, Region 9
75 Hawthorne Street
San Francisco, CA 94105
415/744-1933 (phone)
415/744-1235 (fax)
Brian Hoelscher
IDDEQ
1410 N.Hilton
Boise, ID 83706
208/3 73-0117 (phone)
208/373-0143 (fax)
Thomas Hornshaw
IL Environmental Protection Agency
1021 North Grand Avenue East
Springfield, IL 62794-9276
217/785-0830 (phone)
217/782-1431 (fax)
Bill Horton
ID Department of Fish & Game
P.O. Box 25
Boise, ID 83707-0025
208/334-3791 (phone)
Peter Husby
USEPA
Region 9 Laboratory
1337 S. 46th Street
Building 201
Richmond, CA 94804-4698
510/412-2331 (phone)
Gary Isbell
Ohio Department of Natural Resources
Division of Wildlife
Fountain Square, G-3
Columbus, OH 53224-1837
Rachel Jablonka
USEPA, Region 2
290 Broadway
New York, NY 10007-1866
212/637-3853 (phone)
212/637-3889 (fax)
Matt Kadlec
WA Department of Ecology
Environmental Assessment Program
P.O. Box 47600
Olympia, WA 98504-7600
360/407-6771 (phone)
360/407-6884 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page x of xv
Henry Kahn
USEPA/OST (4303)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-5408 (phone)
202/260-7185 (fax)
Chuck Kanetsky
USEPA, Region 3
1650 Arch Street/3ES 10
Philadelphia, PA 19103-2029
215/814-2735 (phone)
215/814-2782 (fax)
Ronald Klauda
MD Department of Natural Resources
Tawes State Office Building, B-2
580 Taylor Avenue
Annapolis, MD 21401
410/974-3782 (phone)
410/974-2680 (fax)
Kellie Kubena
USEPA, Region 10
1200 Sixth Avenue/OEA-095
Seattle, WA 98101
206/553-1904 (phone)
206/553-0119 (fax)
James P. Kurtenbach
EPADESA
2890 Woodbridge Avenue
Edison, NJ 08837
732/321-6695 (phone)
732/321-6616 (fax)
Richard Langdon
VT DEC Lab
103 S. Main Street
Waterbury, VT 05676
802/241-1379 (phone)
802/241-3008 (fax)
Dore LaRosta
EPADESA
2890 Woodbridge Avenue
Edison, NJ 08837
732/321-6686 (phone)
732/321-6616 (fax)
Robert Maietta
MA Department of Environmental Protection
Office of Watershed Management
627 Main Street (2nd Floor)
Worcester, MA 01608
508/767-2793 (phone)
508/791-4131 (fax)
Randy Manning
GADNR
Environmental Protection Division
745 Gaines School Road
Athens, GA 30605
706/369-6376 (phone)
706/369-6398 (fax)
Dave McKinney
Tennessee Wildlife Resources Agency
P.O. Box 40747
Nashville, TN 37204
615/781-6500 (phone)
Chris Moore
DynCorp, Inc.
6101 Stevenson Avenue
Alexandria, VA 22304
703/461-2360 (phone)
Barry Mower
ME Department of Environmental Protection
17 Statehouse Station
Augusta, ME 04333
207/287-7777 (phone)
207/287-7191 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page xi of xv
Denise Moyer
KY Department of Environmental Protection
Division of Water
Ecological Support Section
14 Reilly Road
Frankfort, KY 40601
502/564-3401 XI56 (phone)
502/564-0111 (fax)
Pete Nolan
New England Regional Lab
60 Westview Street
Lexington, MA 02421
781/860-4343 (phone)
781/860-4397 (fax)
Kevin Novo-Gradac
MD Department of the Environment
2500 Broening Hwy
Baltimore, MD 21224
410/631-3 906 (phone)
Scott Nykerk
CO Division of Wildlife
6060 Broadway
Denver, CO 80216
303/291-7323 (phone)
303/291-7456 (fax)
Tony Olsen
National Health and Environmental Effects
Laboratory/ORD
Western Ecology Division
200 SW 35th Street
Corvallis, OR 97333-4902
541/754-4790 (phone)
541/754-4716 (fax)
John Olson
Iowa Department of Natural Resources
Wallace State Office Building
900 E. Grand Avenue
DesMoines, IA50319
515/281-8905 (phone)
515/281-8895 (fax)
Toney Ott
USEPA, Region 8
999 18th Street (8EPR-EP)
Suite 500
Denver, CO 80202-2466
303/312-6909 (phone)
Jimmie Overton
NC DENR, Division of Water Quality
Ecosystems Analysis Unit
4401 Reedy Creek Road
Raleigh, NC 26707
919/733-9960 (phone)
919/733-9959 (fax)
Randy Parham
OKDEQ
P.O. Box 1677
Oklahoma City, OK 73101-1677
405/702-1026 (phone)
405/702-1001 (fax)
Steve Paulsen
National Health and Environmental Effects
Laboratory/ORD
Western Ecology Division
200 SW 35th Street
Corvallis, OR 97333-4902
541/754-4428 (phone)
541/754-4716 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page xii of xv
Ken Paxton
Ohio DNR
1840 Belcher Drive
Columbus, OH 43221
614/265-6348 (phone)
614/262-1143 (fax)
Eric Pearson
ND Department of Health
Division of Water Quality
1200 Missouri Ave., Rm 203
Bismarck, ND 58506
701/328-5230 (phone)
701/328-5200 (fax)
John Persell
Minnesota Chippewa Tribe
P.O. Box 217
Cass Lake, MN 56633-0217
218/335-6303 (phone)
218/335-8187 (fax)
Esther Peters
Tetra Tech, Inc.
10306 Easton Place, Suite 340
Fairfax, VA 22030
703/385-6000 (phone)
703/385-6007 (fax)
David Petrick
TX Natural Resource Conservation
Commission
Standards and Assessment Section
Water Quality Division
P.O. Box 13087
Austin, TX 78711-3087
512/239-4598 (phone)
512/23 9-4420 (fax)
Ernest Pizzuto
CT Department of Environmental
Protection
Bureau of Water
79 Elm Street
Hartford, CT 06106
860/424-3715 (phone)
860/424-4055 (fax)
Tom Proch,
PADEP/SWRO
400 Waterfront Dr.
Pittsburgh, PA 15222
412/442-4051 (phone)
412/442-4325 (fax)
Walter (Pete) Redmon
USEPA Region 5
77 W. Jackson Blvd./WT15J
Chicago, IL 60604-3507
312/886-6110 (phone)
312/886-0168 (fax)
Amanda Richardson
Tetra Tech, Inc.
10045 Red Run Blvd., Suite 110
Owings Mills, MD 21117
410/356-8993 (phone)
410/356-9005 (fax)
Robert Richardson
RIIDEM
235 Promenade Street
Providence, RI 02908
401/222-4700 x7240 (phone)
401/521-4230 (fax)
Lynn Riddick
DynCorp, Inc.
6101 Stevenson Avenue
Alexandria, VA 22304
703/461-2060 (phone)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page xiii of xv
Bruce Ruppel
NJ Department of Environmental Protection
Division of Science and Research
P.O. Box 409
Trenton, NJ 08625-409
609/984-6548 (phone)
609/292-7340 (fax)
Gary Schiffmiller
NMED
1190 St. Francis Drive
Santa Fe, NM 87502
505/827-2470 (phone)
505/827-0160 (fax)
Lorenzo Sena
EPA Region 7
901 North 5th Street
Kansas City, KS 66101
913/551-7017 (phone)
913/551-7948 (fax)
Dave Serdar
WADept. of Ecology
300 Desmond Drive
Olympia, WA 98504-7600
360/407-6772 (phone)
360/407-6884 (fax)
Tony Shaw
PA DEP/NERO
400 Market Street
Harrisburg, PA 17105
7177787-9637 (phone)
717/787-9549 (fax)
Cindy Simbanin
USEPA/OST (4303)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-5019 (phone)
202/260-7185 (fax)
Don Skaar
MT Department of Fish, Wildlife and Parks
1420 East 6th Avenue
Helena, MT 59620-0701
406/444-5686, 406/444-2449 (phone)
406/444-4952 (fax)
Larry Skinner
NYS Dept of Environmental Conservation
Bureau of Habitat
50 Wolf Road, Rm 576
Albany, NY 12233-4756
518/457-0751 (phone)
518/485-8424 (fax)
Janice E. Smithson, Wildlife Biologist HI
Department of Environmental Protection
Division of Water Resources
1201 Greenbrier Street
Charleston, WV 25311
Phone: (304) 558-2108
Fax: (304)558-2780
jsmithson@mail.dep.wv.state.us
Elaine Snyder
Tetra Tech, Inc.
10045 Red Run Blvd., Suite 110
Owings Mills, MD 21117
410/356-8993 (phone)
410/356-9005 (fax)
Patrick Snyder
Dept of Environment and Natural Resources
523 East Capitol Avenue
Pierre, SD 57501
605/773-4729 (phone)
Betsy Southerland
USEPA/OST (4305)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-3966 (phone)
202/260-9830 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page xiv of xv
JimStahl
IN Department of Environmental
Management
OWM Shade 65-40-2
P.O. Box 6015
Indianapolis, IN 46206-6015
317/308-3187 (phone)
317/308-3219 (fax)
Leanne Stahl
USEPA/OST (4305)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-7055 (phone)
202/260-9830 (fax)
Tom Stauffer
PADEP/NERO
2 Public Square, Suite 151
Wilkes-Barre, PA 18711-0790
570/820-4923 (phone)
570/830-3016 (fax)
Bill Telliard
USEPA/OST (4303)
1200 Pennsylvania Avenue, NW
Washington, DC 20460
202/260-7134 (phone)
202/260-7185 (fax)
Will Tucker
LADEQ
100 AsmaBlvd., Suite 151
Lafayette, LA 70508
318/262-5585 (phone)
318/262-5593 (fax)
Dennis Unkenholz
SD Department of Game, Fish & Parks
523 East Capitol Avenue
Pierre, SD 57501
605/773-4508 (phone)
Mark Warren
NV Division of Wildlife
1100 Valley Road
Reno, NV 89520
775/688-1535 (phone)
775/688-1595 (fax)
Gary Welker
EPA Region 7
901 N. 5th Street
Kansas City, KS 66101
913/551-7177 (phone)
913/551-8752 (fax)
Bob Williamson
ILDNR
524 S. 2nd Street
Springfield, IL 62701
217/782-6424 (phone)
217/785-8262 (fax)
Sherry Wills
PADEP
2 Public Square
Wilkes-Barre, PA 18711-0790
570/826-2553 (phone)
570/830-3016 (fax)
Steve Wolff
Wyoming Game & Fish Department
5400 Bishop Blvd.
Cheyenne, WY 82006
307/777-4673 (phone)
307/777-4677 (fax)
John Woodling
CO Division of Wildlife
6060 Broadway
Denver, CO 80216
303/291-7224 (phone)
303/291-7456 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
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Page xv of xv
Bill Yake
WADept. of Ecology
300 Desmond Drive
Olympia, WA 98504-7600
360/407-6778 (phone)
360/407-6884 (fax)
Edward Younginer
SCDHEC
Bureau of Water Monitoring
Assessment & Protection Division
2600 Bull Street
Columbia, SC 29201
803/734-5401 (phone)
803/734-4435 (fax)
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 1 of 36
A. PROJECT MANAGEMENT
1.0 PROJECT/TASK ORGANIZATION
This Quality Assurance Project Plan (QAPP) describes the quality assurance (QA) and quality control
(QC) activities/procedures that will be used while collecting samples for the National Study of Chemical
Residues in Lake Fish Tissue (hereafter referred to as the National Fish Tissue Study) from 1999
through 2002. The purpose of this document is to present the methods and procedures that will be
used for the collection offish tissue from lakes and reservoirs throughout the United States and the
quality assurance procedures that will be employed. This document addresses only the sample
collection effort of the National Fish Tissue Study.
This QAPP was prepared according to guidance presented in the document EPA Requirements for
Quality Assurance Project Plans, EPA QA/R-5 (USEPA 1999a). Reference to the QAPP elements
described in the guidance document are included herein. The sample collection methods, procedures
and protocols follow the guidelines and recommendations of Guidance For Assessing Chemical
Contaminant Data For Use in Fish Advisories. Volume I: Fish Sampling and Analysis, Second
Edition (USEPA 1995 )[or sub sequent updates].
The project team organization provides the framework for conducting the sample collection task to
meet study objectives. The organizational structure and function also facilitate project performance and
adherence to QC procedures and QA requirements. Key roles are filled by those persons responsible
for ensuring the collection and processing of valid data and for routinely assessing the data for precision
and accuracy, as well as the persons responsible for approving and accepting final products and
deliverables. The project and QA personnel include staff from USEPA and other participating federal
agencies, selected state resource agencies, Native American tribes, and Tetra Tech. The project
organizational chart is presented in Figure 1, and includes relationships and lines of communication
among key project team members.
The USEPA Project Manager is Leanne Stahl, who will supervise the assigned project personnel to
provide for their efficient utilization by directing their efforts either directly or indirectly. As Project
Manager she will also have the following responsibilities:
providing oversight for study design, site selection, and adherence to design objectives,
reviewing and approving the project work plan, QAPP, and other materials developed
to support the project, and
coordinating with contractors, reviewers and USEPA Regions/States/Tribes to ensure
technical quality and contract adherence.
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National Study of Chemical Residues in Lake Fish Tissue QAPP
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Date: 22 May 2000
Page 2 of 36
USEPA Office of Science and
Technology Director
William Telliard
USEPA QA Manager
Esther Peters
Tetra Tech QA Officer
Tetra Tech Field
Sampling Team(s)
Lea
USEPA P
Blaii
Tetra Tec
reject Manager
1
i
:
I
!
1
|
ie Snyder
USEPA State and Tribal
Sampling Field Sampling
Teams Teams
USEPA
Regional/State/Tribal
Fish Sampling
Coordinators
Key
Authority
Lines of Communication
Figure 1. Organizational Diagram for the National Study of Chemical Residues in Lake Fish
Tissue.
The USEPA Quality Assurance Manager is William Telliard, who will be responsible for reviewing
and approving all Quality Assurance Project Plans (QAPPs). Additional USEPA QA Manager
responsibilities include the following:
reviewing and evaluating field procedures,
conducting external performance and system audits of the procedures, and
participating in Agency QA reviews of the study.
The Tetra Tech Task Leader is Blaine Snyder, who will participate in study design and site selection
processes. Other specific responsibilities of the Task Leader include the following:
coordinating project assignments in establishing priorities and scheduling,
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ensuring completion of high-quality projects within established budgets and time
schedules,
providing guidance, technical advice, and performance evaluations to those assigned to
the project,
implementing corrective actions and providing professional advice to staff,
preparing and/or reviewing preparation of project deliverables, and
providing support to USEPA in interacting with the project team (including the sample
control center), technical reviewers, and USEPA Regions/States/Tribes to ensure
technical quality requirements are met in accordance with project design objectives.
The Tetra Tech Quality Assurance (QA) Officer is Esther Peters, whose primary responsibilities
include the following:
monitoring quality control (QC) activities to determine conformance,
reviewing the QAPP for completeness and noting inconsistencies,
providing support to USEPA and the Tetra Tech Task Leader in preparation of the
work plan and QAPP and in their distribution, and
approving the QAPP.
The Regional Fish Sampling Coordinators or QA/QC Field Officers will be responsible for
performing evaluations to ensure that QA/QC protocols are maintained throughout the sample
collection and preparation processes. The evaluations will include reviewing all required documentation
for completeness and seeing that any problems encountered outside normal operating conditions are
documented and addressed, and verifying all other QA/QC procedures identified in the QAPP are
followed. The USEPA Project Manager and the Tetra Tech Task Leader will coordinate and oversee
the orientation of the Regional Fish Sampling Coordinators or QA/QC Field Officers responsible for
USEPA Regional/State/Tribal Field Sampling Teams.
Field Sampling Teams will be composed of:
USEPA field staff, and/or
State and Tribal field personnel, and/or
Contractor-affiliated field staff (including subcontracted organizations or universities).
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The Task Leader will direct and supervise the contractor-affiliated Field Sampling Teams and provide
for their efficient utilization by directing their efforts. Both agency and contractor-affiliated field
personnel are responsible for performing the field work, including collection, preparation, and shipment
offish tissue samples and completion of field sampling records. The Field Sampling Teams will include
scientific staff with specialization and technical competence in field sampling activities to effectively and
efficiently perform the required work. They must perform all work in adherence with the project work
plan and QAPP, including maintenance of sample custody and related documentation. Custody
procedures are required to ensure the integrity of the samples with respect to prevention of
contamination and maintenance of proper sample identification during handling. In this role, Field
Sampling Teams are responsible for:
receiving and inspecting the sample containers,
completing and signing appropriate field records,
assigning tracking numbers to each sample,
verifying the completeness and accuracy of chain-of-custody documentation,
controlling and monitoring access to samples while in their custody, and
initiating shipment of the samples to appropriate destinations.
2.0 PROBLEM DEFINITION/BACKGROUND
The USEPA Office of Water conducted a national screening-level investigation in 1987 (USEPA
1992) to determine the prevalence of selected bioaccumulative pollutants in fish and to correlate
elevated fish tissue contaminant levels with pollutant sources. Gamefish and bottom-dwelling fishes
were collected from 388 locations across the country thought to be influenced by various point and
nonpoint sources. These fish tissue samples were analyzed to determine levels of 60 target analytes,
including dioxins and furans, PCBs, pesticides and herbicides, mercury, and several other organic
compounds. Results of the 1987 study indicated that target analytes were present in fish tissue at many
of the sampling sites, and some of the contaminants (e.g., PCBs, dieldrin, mirex, and combined
chlordane) occurred at levels posing potential human health risks.
The Office of Science and Technology (OST) within the Office of Water has initiated work on a new
four-year national study of chemical residues in fish tissue, which is designed to expand the scope of the
1987 study. In October 1998, USEPA convened a two-day workshop of more than 50 scientists from
state, federal, and tribal agencies to obtain technical input on sampling design, target analytes, sampling
methods and data management. Input from scientists at the workshop and other technical experts that
participated in numerous study planning meetings was used to develop a final study design (USEPA
1999b). The contemporary study is statistically designed and will provide screening-level data on fish
tissue contaminants from a greater number of waterbodies than were sampled in 1987.
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This study broadens the scope of the 1987 study (USEPA 1992) which focused on chemical residues
in fish tissue near point source discharges. The new study will:
provide information on the national distribution of selected persistent, bioaccumulative,
and toxic (PBT) chemical residues in gamefish and bottom-dwelling fish in lakes and
reservoirs of the coterminous United States (excluding the Great Lakes and the Great
Salt Lake),
include lakes and reservoirs selected according to a probability design,
involve the collection offish from those randomly selected lakes and reservoirs over a
four-year survey period (1999-2002),
not be used to set fish consumption advisories; however, states and Native American
tribes may choose to initiate a detailed fish study in a particular lake based on the
screening contaminant concentrations provided by the national study, and
include the analysis offish tissue for PBT chemicals selected from USEPA's multimedia
candidate PBT list of 451 chemicals and from a list of 130 chemicals from several
contemporary fish and bioaccumulation studies. A final target analyte list of 274 PBT
chemicals (including breakdown products and PCB congeners) was compiled based on
input from study design workshop participants and a review team of analytical experts
convened in October 1998 and March 1999, respectively.
Lakes and reservoirs were chosen as the target population because they:
are accumulative environments where contamination is detectable,
provide important sport fisheries nationwide,
offer other recreational (non-fishing) access and opportunities, and
occur in agricultural, urban, and less-developed areas, so that associations with each
primary use may be determined.
Lakes and reservoirs are the focus of this study rather than other waterbody types because:
Fish consumption advisories represent 15.8% of the Nation's total lake acres (plus
100% of the Great Lakes), compared to 6.8% of the Nation's total river miles
(USEPA 1999c). [Note: The Great Lakes will not be included in this study because
substantial fish tissue contaminant information is available and continues to be collected
in ongoing Great Lakes monitoring programs.]
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Estuaries are currently being studied by USEPA's Environmental Monitoring and
Assessment Program (EMAP). EMAP has sampled fish from East and Gulf Coast
estuaries, and will include fish contamination in its Year 2000 initiative on West Coast
estuaries.
The specific objective of the new National Fish Tissue Study is to estimate the national distribution
of the mean levels of selected persistent, bioaccumulative, and toxic chemical residues in fish
tissue from lakes and reservoirs of the continental United States.
In so doing, the study will provide the following types of information:
information to meet objectives of the President's Clean Water Action Plan (CWAP)
and to specifically respond to the following action item:
CWAP Key Action #1: USEPA and NOAA will conduct a national survey of
mercury and other contaminants in fish and shellfish throughout the country, and
will coordinate the effort with states and tribes to maximize geographic
coverage. The shellfish survey will be based on the data obtained by NOAA's
ongoing Mussel Watch Project.
information about persistent, bioaccumulative, and toxic chemicals (PBTs) for the
Agency's PBT Initiative that addresses the following objective:
The PBT Initiative seeks to identify areas of concern for human and/or
ecological health. Study offish tissue may reveal where PBTs not previously
considered a problem are present at levels of concern.
data to answer important questions concerning the national occurrence of fish tissue
contamination, such as the following:
What is the national extent of selected chemical contaminants in fish from lakes
and reservoirs of the coterminous United States (excluding the Great Lakes)?
Are contaminant levels in fish high enough to warrant further investigation?
3.0 PROJECT/TASK DESCRIPTION
The study design reflects the study goal and objectives defined by USEPA. The study goal can be
stated simply to determine the extent to which fish in waters of the United States are contaminated
with persistent, bioaccumulative, and toxic chemicals (PBTs). The project field sampling task
presented and discussed in this document involves only those methods and
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procedures used to collect and ship fish tissue samples for the National Fish Tissue Study. The
Analytical Activities QAPP for the National Fish Tissue Study discusses the following study topics and
tasks: sample preparation, compositing and homogenization; target analytes; analytical methods; and
sample analysis.
In consultation with the USEPA Office of Science and Technology, Tetra Tech will coordinate with
USEPA headquarters and regional staff, state resource agencies, and Native American tribes to collect
fish tissue samples from randomly selected lakes and reservoirs in the continental United States. With a
combined network of partners and contractors, USEPA anticipates the sampling of approximately 500
lakes across the country (Appendix A) during the four-year sampling duration of the study (1999-
2002). The fish tissue samples will be collected based on a probability design to provide information on
national distribution of the mean levels of contaminants in fish. This random selection of lakes and
reservoirs is important for fulfilling the study design objectives, but adds complexity to field sampling
logistics. Sampling Teams will need to be prepared to mobilize and sample fish from lakes in all parts
of the country. The following elements will also add to the complexity of the field effort, and must be
considered when planning field logistics:
Field teams should consist of (at a minimum) one experienced fisheries biologist, one
field technician, and a quality control specialist, all of whom must have experience with
the array of fisheries sampling gear types to be used. In some cases the senior fisheries
biologist may serve in dual capacities, assuming responsibility for site quality control
(QC).
The national study will include two groups of target fishes predator/gamefish and
bottom-dwelling fish species (Section 8.1).
Samples must consist of a composite offish (e.g., 5 individuals that will collectively
provide greater than 560 grams of edible tissue for predators and 560 grams of total
body tissue for bottom-dwellers) of the same target species and be the same relative
size from each sampling location (Section 8.2).
The optimum sampling window may be restricted due to biological, physical, and
meteorological conditions and factors (Section 7.2).
Each Sampling Team, in the combined network of samplers, will collect, prepare for shipment, and ship
all fish tissue samples to a designated location according to the methods and procedures described in
this QAPP and approved by the USEPA Project Manager. The USEPA Project Manager will be
notified immediately by the Tetra Tech Task Leader and/or the USEPA Regional/State/Tribal Fish
Sampling Coordinators of any problems related to successful
completion of field efforts.
Field sampling activities began in the fall of 1999, will continue in 2000 and 2001 during the summer
and fall, and will conclude in the fall of 2002. Due to the effort required to initiate the
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study (e.g., project coordination and development of study materials such as the study design
document, QAPP, and training materials), fewer lakes were sampled in year one than will be sampled
during other sampling years. In addition, some states indicated that they were unable to participate in
sampling during the first year, but will have adequate time to prepare for sampling in subsequent years
of the study. Data summaries for the four-year period of study and the final study report are scheduled
to be completed in 2003. Implementation of the field sampling task will proceed with several
milestones, as presented in Table 1.
All activities associated with fish tissue sample collection will be conducted consistent with the
requirements and procedures specified in this QAPP as approved by the USEPA Project Manager.
Annual sampling activities will conclude with the development of a field collection effort summary (i.e.,
detailed listing of all sampling participants, sampling locations, and specimens collected) by Tetra Tech
and reviewed by the USEPA Project Manager. The summaries will be used to document and report
back to USEPA Regional/State/Tribal participants the collective sampling progress for each study year.
Table 1. Project Time Line for Milestones Associated with Fish Tissue Sample Collection
Activities.
Activities and
Milestones
(1999 - 2002)
Develop QAPP and
project sampling plan
for field efforts
Conduct orientation
for sampling and field
QC personnel in EPA
Regions
Sampling of lakes
and shipment of
samples to analytical
laboratory
Field collection effort
summaries and
report-back to
regions, states, and
tribes
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4.0 QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT DATA
4.1 Project Quality Objectives
Data of known and documented quality are essential to the success of any monitoring or sampling
program. Data quality objectives (DQOs) are qualitative and quantitative statements that clarify the
intended use of the data, define the type of data needed to support the decision, identify the conditions
under which the data should be collected, and specify tolerable limits on the probability of making a
decision error due to uncertainty in the data. DQOs are developed by data users to specify the data
quality needed to support specific decisions. Sources of error or uncertainty include the following:
Sampling error: The difference between sample values and in situ true values from
unknown biases due to collection methods and sampling design,
Measurement error: The difference between sample values and in situ true values
associated with the measurement process,
Natural variation: Natural spatial heterogeneity and temporal variability in population
abundance and distribution, and
Error sources or biases associated with compositing, sample handling, storage, and
preservation.
This QAPP addresses only fish tissue sample collection activities, so the relevant quality objectives are
primarily related to sample handling issues. One exception involves the measurement of lake pH.
Study DQOs for pH will require that meters are calibrated to a known standard as per manufacturer's
specifications (Appendix C). Types of field sampling data needed for this project are listed in Table 2.
Discussion of conventional data quality indicators, i.e., precision, accuracy, completeness,
representativeness, and comparability, follows in this section. Methods and procedures described in
this document are intended to reduce the magnitude of the sources of uncertainty (and their frequency
of occurrence) by applying the following approaches:
use of standardized sample collection and handling procedures, and
use of trained scientists to perform the sample collection and handling activities.
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Table 2. Types of Field Data to Be Collected in Association with Fish Tissue Sample
Collection.
Data Type
Fish specimen
Fish length
Composite classification
PH
Estimated maximum lake depth
Measurement Endpoint(s) or Units
Species-level taxonomic identification
Millimeters (mm), total length
Predator or bottom-dwelling species
nearest 0. 1 pH units
Meters
4.2 Measurement Performance Criteria
Measurement performance criteria are quantitative statistics that are used to interpret the degree of
acceptability or utility of the data to the user. These criteria, also known as data quality indicators
(DQIs), include the following:
precision,
accuracy,
representativeness,
completeness, and
comparability.
Precision
Precision is a measure of internal method consistency. It is demonstrated by the degree of agreement
between individual measurements (or values) of the same property of a sample, measured under similar
conditions. As the analytical testing is beyond the scope of this QAPP, no specific criteria are required
for this parameter. However, sufficient sample volumes (i.e., the five-fish composites described in
Section 8.2) will be collected to allow for the assessment of precision during analytical laboratory
testing.
For this study, all fish in a lake cannot be sampled, and the laboratory analytical process is not perfect.
The combined variability introduced by the sampling at a lake, the compositing offish, the subsampling
of the composite for analysis, and the chemical analysis itself can be considered the "index" variability.
The detection limits and analytical precision are one part of the
analytical process that can be specified ahead of time (however analytical processes are not part of this
QAPP). The orientation and training of sampling crews, and the process that they use to collect fish
from a lake can also be standardized. Besides standardizing training, this dimension of variability cannot
be reduced. The general rule of thumb is that if the combined index variability is less than 10% of the
total variability, it will have little impact on the ability to estimate status. For this study the best way to
develop an estimate of index variability is to simply revisit a subset, 10% of the sites, and repeat the
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lake sampling procedure, compositing and analytical analyses. Sampling teams should plan to obtain
duplicate fish samples from 10% of the target lakes and reservoirs in their state during the four-year
study period.
Accuracy
Accuracy is defined as the degree of agreement between an observed value and an accepted reference
or true value. For example, accuracy of pH meters used for this study will be assured through proper
calibration to known standards, i.e., buffer solutions (Appendix C). Accuracy is a combination of
random error (precision) and systematic error (bias), introduced during sampling and analytical
operations. Bias is the systematic distortion of a measurement process that causes errors in one
direction, so that the expected sample measurement is always greater or lesser to the same degree than
the sample's true value. As mentioned previously, since analytical testing is beyond the scope of this
QAPP, no accuracy criteria are identified here. However, proper sample handling procedures (Section
9.1) will be followed to minimize sample contamination.
Representativeness
Representativeness expresses the degree to which data accurately and precisely represent a
characteristic of a population, parameter, variations at a sampling point, a process condition, or an
environmental condition.
Representativeness of the target species (Section 8.1) for this fish tissue sampling effort was established
based on:
the recommendation of USEPA's Guidance for Assessing Chemical Contaminant
Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second
Edition (USEPA 1995),
the input from federal, state, and tribal scientists on the draft design of the National
Study of Chemical Residues in Lake Fish Tissue, obtained primarily during the October
1998 workshop (USEPA 1999b), and
approval by the USEPA Proj ect Manager.
The representative goal for the sample collection effort will be satisfied by using experienced field
biologists to ensure that the sample types and locations specified for the study are the samples actually
collected.
Completeness
Completeness is defined as the percentage of measurements made that are judged to be valid according
to specific criteria and entered into the data management system. To optimize completeness, every
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effort is made to avoid sample and/or data loss. Accidents during sample transport or lab activities that
cause the loss of the original samples will result in irreparable loss of data, which will reduce the ability
to perform analyses, integrate results, and prepare reports. Samples will be stored and transported in
unbreakable (plastic) containers (i.e., insulated ice chests). All sample processing (i.e., compositing,
filleting, homogenization) will occur in a controlled environment within the laboratory, not in the field.
The assignment of a set of specific sample numbers (Section 6.0) that have undergone chain-of-custody
inspection makes it less likely for the sample preparation laboratory to overlook samples when
preparing them for processing.
Percent completeness (%C) for measurement parameters can be defined as follows:
Where v = the number of measurements judged valid and
T = the total number of measurements.
Completeness, in the case of this project, is the number of valid samples collected relative to the
number of samples that are planned to be collected. The completeness goal for this project is 90%. It
should be noted that sample locations and numbers may change over the course of the four-year study,
based on local conditions (e.g., accessibility of target lakes) and the availability of target fishes (e.g.,
natural biological abundance or distribution). Any and all changes must be approved by the USEPA
Project Manager, and approved changes must be considered when assessing completeness. The
completeness goal is achieved when 90% or more of the available samples from the final list of target
lakes found to contain target fishes are collected and shipped with no errors in documentation or
sample handling procedures.
Comparability
Comparability is an expression of the confidence with which one data set can be compared with
another. Comparability is dependent on the proper design of the sampling program and on adherence
to accepted sampling techniques, standard operating procedures, and quality assurance guidelines. For
the fish tissue collection task, comparability of data will be accomplished by standardizing the sampling
season, the field sampling methods, and the field training as follows:
All samples will be collected during the late summer-fall (August-November).
All samples will be collected and prepared for shipment according to standard
operating procedures contained in this QAPP. These procedures are consistent with
the recommendations of USEPA's Guidance for Assessing Chemical Contaminant
Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second
Edition (USEPA 1995 )[or sub sequent updates].
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All field personnel involved with sampling will have adequate training and appropriate
experience (Section 5.0).
5.0 SPECIAL TRAINING REQUIREMENTS/CERTIFICATION
Each Field Sampling Team is required to have the necessary knowledge and experience to perform all
field activities. This includes both knowledge and experience in the collection and identification of
fishes, in the use of fisheries sampling gear specified for the study and in the operation of small boats. It
also includes training in project-specific sample collection and handling procedures. The field sampling
crews will be primarily composed of state, tribal, and regional fisheries biologists or contracted
biologists with a strong technical background in fisheries sampling activities. Each Field Sampling Team
should consist of (at a minimum) one experienced fisheries biologist, one field technician, and a quality
control specialist, all of whom must have experience with the array of fisheries sampling gear types to
be used. In some cases the senior fisheries biologist may serve in dual capacities, assuming
responsibility for site quality control.
This field sampling QAPP, the field sampling plan, and orientation materials will be distributed to all
USEPA Regional/State/Tribal Fish Sampling Coordinators, who will, in turn, distribute it to all sampling
personnel. Project orientation sessions will be set up by EPA Regions to distribute and discuss training
materials. Materials will include detailed instructions for each field procedure (i.e., sampling of target
fish, proper handling of the sample, shipping, and chain of custody) and visual training tools based on
information from this QAPP. The focus of the orientation will be on sample collection methods, specific
details of sample preparation, and strict adherence to the study's protocols. USEPA
Regional/State/Tribal Fish Sampling Coordinators and Field Team Leaders will be required to view the
training materials, read the QAPP, and verify in writing that they read or viewed the materials and
understood the procedures and requirements. If sampling personnel change (i.e., new USEPA
Regional/State/Tribal Fish Sampling Coordinators or new Field Team Leaders) during the course of the
four-year study, the orientation process will have to be reinitiated for that particular new team.
6.0 DOCUMENTATION AND RECORDS
Thorough documentation of all field sample collection and handling activities is necessary for proper
processing in the laboratory and, ultimately, for the interpretation of study results. Field sample
collection and handling will be documented in writing (for each sampling site) using the following forms
and labels:
a Field Record Form that contains information about each individual specimen and lake
site (Appendix B),
a Sample Identification Label that accompanies and identifies each sample (Appendix
B),
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a Chain-of-Custody Label that seals each sample container (provided by the sample
control center), and
a Chain-of-Custody Form that provides constant tracking information for all samples
(Appendix B).
A detailed description of each sample collected by each Field Sampling Team will be recorded on a
Field Record Form (Appendix B). The form will document the sampling date, time, sampler's name,
sampling site location/description, and sample description (count and length of each specimen). Also,
the 10% subset of lakes and reservoirs that are sampled as duplicates will be noted as such on the
form. The Field Record Form will also contain a unique tracking code (i.e., composite sample
identification code) that will be used to identify each record. The ten-character code will include:
state of collection (two-character abbreviation),
year of collection (two-number abbreviation),
lake identification number (four-digit code from Appendix A),
composite type (one character P = predator species; B = bottom-dwelling species),
and
sample type (one character - S = standard sample, D = duplicate sample).
The Field Record Form will be produced as a four-page carbonless copy form, with one copy retained
by the sampler, and the other three included in the sample shipment to the laboratory (i.e., one for the
sample preparation laboratory, one for the sample control center, and one for the Tetra Tech Task
Leader). All entries will be made in ink and no erasures will be made. If an incorrect entry is made, the
information will be crossed out with a single strike mark, which is initialed and dated by the
sampler/recorder.
A Sample Identification Label will be completed (Appendix B) to accompany each sample throughout
the chain of custody. The label will document the project name, sampling site location, sampling date
and time, the sampler's name, the ten-character tracking code, and the specimen number (e.g., 01
through 05). All entries will be made in indelible ink and will coincide with specimen and sample
information on the Field Record Form. Descriptions and definitions of all field data elements required in
the Field Record Form and Sample Identification Label are provided in Appendix D.
Proper chain-of-custody procedures are necessary for tracking sample possession from field to
laboratory. Chain-of-Custody Forms (Appendix B) will accompany each shipment of samples and will
document sample identity (coinciding with information on the field record), sampler relinquishment date
and time, and laboratory receipt date and time. Chain-of-Custody Forms will be produced as five-
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page carbonless copies, with one copy for the sampler, and four for shipment to the laboratory (i.e.,
one for the sample preparation laboratory, one for the sample control center, one for the Tetra Tech
Task Leader, and one for duplication and distribution to the analytical laboratories). Chain-of-Custody
Labels will seal each sample container following packing operations in the field, and will include the
signature of the sampler and the date and time sealed. All Chain-of-Custody Label and Form entries
will be made in ink. Field sampling teams must notify the sample control center (DynCorp) by
telephone (Chris Moore 703/461-2360 or Chris Maynard 703/461-2395) of an incoming shipment.
Samples will be shipped from the field to the sample preparation laboratory via priority, overnight
express delivery service. Copies of all shipping airbills will be retained by the sample control center.
Specification for retention of field samples by the receiving location are outside the scope of this
document. While in storage, it is recommended that samples held for analysis be stored with the
original labeling materials.
Annual sampling activities will conclude with the development of a field collection effort summary (i.e.,
detailed listing of all sampling participants, sampling locations, and specimens collected) by Tetra Tech
and review of the summary by the USEPA Project Manager. Following USEPA Project Manager
approval, the summaries will be used to document and report back to USEPA Regional/State/Tribal
participants the collective sampling progress for each study year. Tetra Tech will maintain a file as a
repository for information used in the preparation of the annual field collection summaries throughout the
duration of the study. The following information will be included:
any documents prepared for the study,
contract and work assignment information,
project QAPP,
results of technical reviews, data quality assessments, and audits,
communications (memoranda; internal notes; telephone conversation records; letters;
meeting minutes; and all written correspondence between Tetra Tech, USEPA, and
other project team personnel, subcontractors, suppliers, or others),
maps, photographs, and drawings, and
studies, reports, and documents pertaining to the project.
If any change(s) in this QAPP is(are) required during the study, a memo will be sent to each person on
the distribution list describing the change(s), following approval by the USEPA Project Manager. Any
and all memos announcing changes must be attached to the QAPP.
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All documents and records prepared for this project will be maintained by USEPA and Tetra Tech
during the project, and retained for a period of two years following completion of the project (unless
otherwise directed by USEPA).
B. DATA ACQUISITION
7.0 SAMPLING PROCESS DESIGN
The objective of the National Fish Tissue Study is to estimate the national distribution of the mean levels
of selected persistent, bioaccumulative, and toxic chemical residues in fish tissue from lakes and
reservoirs of the continental United States.
In so doing, the study will provide the following types of information:
information to meet objectives of the President's Clean Water Action Plan (CWAP),
information about persistent bioaccumulative toxic chemicals (PBTs) for the Agency's
PBT Initiative, and
data to answer important questions concerning the national occurrence of fish tissue
contamination.
For the purposes of this study design, the target population will be all lakes and reservoirs within the
coterminous United States excluding the Laurentian Great Lakes and the Great Salt Lake. This study
defines a lake as a permanent body of water of at least one hectare (2.47 acres) in surface area with a
minimum of 1,000 m2 of open (unvegetated) water and a minimum depth of one meter. The lakes in
this study must also have a permanent fish population. Approximately 500 locations will be sampled
over the course of four years based on projections of available resources.
7.1 Sample Type
To meet the study objectives, the National Fish Tissue Study will include composite sampling offish
fillets for predator/gamefish species and whole fish for bottom-dwelling species from each sample lake.
Five individuals per composite will be collected, all of which will be large enough to provide sufficient
tissue for analysis of the group of target analytes. It has been determined that at least 560 grams of
edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers will be required
from the composites to allow for analysis of all target analytes. Based on the recommendations of
USEPA's Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories,
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Volume 1: Fish Sampling and Analysis, Second Edition (USEPA 1995), fish used in a composite
sample must meet the following criteria:
all be of the same species,
satisfy any legal requirements of harvestable size or weight, or at least be of consumable
size if no legal harvest requirements are in effect,
be of similar size so that the smallest individual in a composite is no less than 75% of the
total length of the largest individual,
be collected at the same time (i.e., collected as close to the same time as possible but
no more than 1 week apart) [Note: This assumes that a sampling crew was unable to
collect all fish needed to prepare the composite sample on the same day. If organisms
used in the same composite are collected on different days (no more than 1 week
apart), individual fish will be frozen until all the fish to be included in the composite are
available for delivery to the laboratory.], and
be collected in sufficient numbers (five per composite) and of adequate size (five
harvestable size adult specimens that collectively will provide greater than 560 grams of
edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers) to
allow analysis of recommended target analytes.
Individual organisms used in composite samples must be of the same species because of notable
differences in the species-specific bioaccumulation potential. Accurate taxonomic identification is
essential in preventing the mixing of closely related species with the target species. Under no
circumstance should individuals from different species be used in a composite sample.
7.2 Sampling Period
Field sampling will be conducted during the period when water and weather conditions are conducive
to safe and efficient field sampling, and when the target species are most frequently harvested by
anglers. For most inland freshwaters, the most desirable sampling period is from late summer to early
fall, since lipid content is usually highest and water levels are usually lowest at that time. Sampling
should not occur during the spawning period of the particular target species being sought. With these
recommendations in mind, and considering the geographic extent of the study area (i.e., range of
latitudes and longitudes) the field sampling period will begin in August and last through November (and
possibly into December in warmer regions).
7.3 Sample Frame
For the purposes of this study, the target population will be all lakes and reservoirs within the
coterminous United States excluding the Laurentian Great Lakes and the Great Salt Lake. For this
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study, a lake is defined as a permanent body of water of at least one hectare (2.47 acres) in surface
area with a minimum of 1,000 m2 of open (unvegetated) water, and a minimum depth of one meter.
The lakes in this study must also have a permanent fish population. Examples of nonpermanent fish
populations are lakes that are subject to annual fish winterkill, or are recently
stocked with fingerlings. Stocked lakes with adult fish are defined as having a permanent fish
population.
The River Reach File Version 3 (RF3) was used to generate the list of lakes in the target population.
RF3 constitutes the sample frame, and includes almost all lakes in the target population for this study.
Noted exclusions are newly constructed reservoirs. However, RF3 is the best known national GIS
coverage for lakes, so it was used in this study.
To ensure the sample frame included all lakes and reservoirs with an area greater than 5,000 ha, a list
from multiple sources of such lakes was constructed. The list was sent to USEPA Regional Offices and
subsequently to each state to verify that each lake on the list was greater than 5,000 ha and to add any
lakes greater than 5,000 ha that were not on the list. The corrected list of lakes was integrated into the
RF3 list of lakes before sample selection was initiated. Table 3 summarizes the number of lakes in the
sample frame used for sample selection.
Table 3.
Numbers of Lakes by Size Category in Sample Frame (Based on RF3).
Lake area (ha)
>l-5
>5-10
>10-50
>50-500
>500-5000
>5000
Number of Lakes
172,747
44,996
40,016
11,228
1,500
274
Frequency (%)
63.8
16.6
14.8
4.1
0.6
0.1
Cumulative
Number of Lakes
172,747
217,743
257,759
268,987
270,387
270,761
Cumulative
Frequency (%)
63.8
80.4
95.2
99.3
99.9
100.0
7.4 Selection of Lakes for Sampling
The procedures described by Olsen et al. (1998) were used to select an unequal probability sample of
lakes. The probability of selection for a lake depends on its area as given by RF3. In Table 4 the
expected weight is the reciprocal of the probability of selection (inclusion probability). The inclusion
probability was determined by the goal of obtaining approximately an equal number of lakes to sample
in each size category. A higher percentage of the lakes in the smaller size categories would include
lakes not meeting the target population definition of a lake. The probability of selection was adjusted so
that the smaller size categories had a greater sample size. No adjustment was required for size
categories 50-500 hectares, 500-5000 hectares, or > 5000 hectares. The adjustments for the
remaining size categories were as follows: for 1-5 hectares, increase by 40%; for 5-10 hectares,
increase by 30%; and for 10-50 hectares, increase by 20%. These adjustments were based on limited
information from the EMAP northeastern lake survey. It is not known yet how well these will apply to
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other regions of the country. The impact of an incorrect adjustment will be that the number of lakes
actually sampled by size category will not be equal.
Although it was not a requirement for the statistical survey design, study planners decided to select the
sample by allocating the lakes to be sampled in each year of the study. It is recommended that the
lakes be sampled in the year specified. The advantage of adhering to this approach is that if any year-
to-year differences exist in fish tissue contaminants, then the sample will be balanced across years. In
the event that the study must be stopped before all lakes can be sampled, sampling all lakes from a
subset of the years (e.g., 1999-2001) results in a legitimate unequal probability sample of all lakes. The
expected weights must be adjusted to account for the years not sampled.
Table 4.
Number of Lakes Selected for Sampling by Size Category and Year.
Lake area
(ha)
>l-5
>5-10
>10-50
>50-500
>500-5000
>5000
Total
1999
39
44
32
34
36
40
225
2000
41
40
47
37
30
30
225
2001
47
47
46
29
31
25
225
2002
47
46
25
34
41
32
225
All Years
174
177
150
134
138
127
900
Expected
Weight
938.84
261.61
256.51
85.06
11.36
2.21
7.5 Nontarget Population, Inaccessible Lakes, and Lakes for which Access is Denied
A critical element of the statistical survey design is the determination of the status of each lake in the
sample. This means that each lake is checked to determine if it meets the definition of a lake for the
study (Section 7.3). In many cases, a field visit is not necessary to confirm that the lake meets the
definition. In other cases, it may be necessary to actually visit the lake to determine if it meets the
definition. Regardless, it is essential that a complete record of this information be reported to the
USEPA Project Manager, since this information is required to complete the survey estimation
procedures. Two other situations can occur that will result in a lake not being sampled. First, the lake
may be on private land and require landowner permission to visit the lake. If a landowner refuses
access to a lake selected for the study, then this needs to be recorded. Second, a lake may
occasionally be physically inaccessible. If there are logistical or safety constraints that make a lake
inaccessible, then the reason why the lake is inaccessible needs to be recorded and reported to the
USEPA Project Manager and/or the Tetra Tech Task Leader.
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Information that must be determined during pre-sampling reconnaissance of each lake includes the
following:
Does the lake meet the definition of the target population (Section 7.3)? If the lake
does not meet the definition, what are the reasons? For example:
lake < 1 ha in surface area
lake < 1 m depth
lake < 1000 m2 of open water (unvegetated)
saline lake with no fish population
lake has no annual fish population (winterkill lake)
other (list specific reasons)
Has the landowner denied access to lake? (Record landowner information)
Is the lake physically inaccessible during sampling period of study? If so, state why.
7.6 Reserve Sample of Lakes
As a contingency, a second sample of lakes has been selected as a reserve. Table 5 summarizes the
sample sizes for the reserve sample. This sample could be used if the initial sample is determined to
have a larger than expected number of nontarget population lakes, resulting in an insufficient sample
size. Alternatively, if additional funding is received to allow a larger sample size, the reserve sample of
lakes could be used. Decisions regarding use of the reserve sample of lakes (or subsets of the reserve
sample) will be made only by the USEPA Project Manager.
Table 5.
Number of Lakes (by Size Category and Year) Selected as a Reserve Sample.
Lake area (ha)
>l-5
>5-10
>10-50
>50-500
>500-5000
>5000
Total
1999
47
45
36
36
38
23
225
2000
48
52
39
26
29
31
225
2001
48
40
42
40
30
25
225
2002
49
42
41
22
37
34
225
All Years
192
179
158
124
134
113
900
Expected Weight
938.84
261.61
256.51
85.06
11.36
2.21
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8.0 SAMPLING METHODS
8.1 Target Species
Field sampling procedures will follow the recommendations of USEPA's Guidance for Assessing
Chemical Contaminant Data for Use in Fish Advisories, Volume I: Fish Sampling and Analysis,
Second Edition (USEPA 1995) [or subsequent updates]. According to the guidance, the primary
criteria for selecting target fishes is that the species:
are commonly consumed in the study area,
may potentially accumulate high concentrations of chemicals, and
have a wide geographic distribution.
Secondarily, the target species should be:
easy to identify,
abundant,
easy to capture, and
large enough to provide adequate tissue for analysis (i.e., harvestable size adult
specimens that as a five-fish composite will provide at least 560 grams of edible tissue
for analysis).
Two distinct ecological groups offish, bottom-dwellers and predators, will be included as target fishes
for this study. This permits monitoring of a wide variety of habitats, feeding strategies, and physiological
factors that might result in differences in bioaccumulation of contaminants. Suggested target species are
listed in Table 6 in order of preference (adapted from USEPA 1995). Additional target species may
be added to the list of preferred targets on an as-needed basis, following discussion with the USEPA
Project Manager and/or the Tetra Tech Task Leader. For example, additional Salmonid species (such
as cutthroat trout or kokanee salmon) and Catostomid species (such as longnose sucker, largescale
sucker, or bridgelip sucker) may need to be added to the target species for lakes in the northwestern
United States. State personnel, with their knowledge of site-specific fisheries and human consumption
patterns, will aid in the determination of the availability of target fishes. The criteria listed above must be
considered when selecting target species other than those listed in Table 6. Every effort will be made to
collect the desired species and number (Section 8.2) offish; however, the outcome of field sampling
efforts will ultimately depend on the natural diversity and abundance offish in the study lakes.
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8.2 Composite Sampling
The National Fish Tissue Study will involve composite sampling of predator/gamefish species and
bottom-dwelling species (to be prepared as fillet composites and whole-body composites, respectively,
by the sample preparation laboratory). Composite samples are cost-effective for
estimating average tissue concentrations of target analytes in target species populations, and
compositing ensures adequate sample mass for analysis of all target analytes.
Table 6.
Recommended Target Species for Inland Freshwaters (in Order of Preference).
11
If
I -2-
1 2
E 3
Ł o
2Ł
Family name
Centrarchidae
Percidae
Percichthyidae
Esocidae
Salmonidae
Cyprinidae
Ictaluridae
Catostomidae
Common name
Largemouth bass
Smallmouth bass
Black crappie
White crappie
Walleye
Yellow perch
White bass
Northern pike
Lake trout
Brown trout
Rainbow trout
Brook trout
Common carp
Channel catfish
Blue catfish
Brown bullhead
Yellow bullhead
White sucker
Scientific name
Micropterus salmoides
Micropterus dolomieu
Pomoxis nigromaculatus
Pomoxis annularis
Stizostedion vitreum
Percaflavescens
Morone chrysops
Esox lucius
Salvelinus namaycush
Salmo trutta
Oncorhynchus mykiss
Salvelinus fontinalis
Cyprinus carpio
Ictalurus punctatus
Ictalurus furcatus
Ameiurus nebulosus
Ameiurus natalis
Catostomus commersoni
One predator/gamefish composite and one bottom-dwelling species composite will be collected from
each target lake (Note: The USEPA Project Manager and/or the Tetra Tech Task Leader need to be
notified if one of the ecological groups offish are not present or available from a target lake). Each
composite will consist of five fish of adequate size (i.e., adult specimens that collectively will provide at
least 560 grams of edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers)
to allow analysis of the target analytes. Fish retained for a composite sample must meet the following
criteria:
all be of the same species,
satisfy any legal requirements of harvestable size (or weight), or at least be of
consumable size if no legal harvest requirements are in effect,
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be of similar size so that the smallest individual in a composite is no less than 75% of the
total length of the largest individual, and
be collected at the same time, i.e., collected as close to the same time as possible, but
no more than one week apart (Note: Individual fish may have to be frozen until all fish
to be included in the composite are available for delivery to the sample preparation
laboratory).
Accurate taxonomic identification is essential in assuring and defining the organisms that have been
composited and submitted for analysis. Under no circumstances should individuals from different
species be used in a single composite sample. Ideally, the target species composite should focus on the
larger individuals commonly harvested by the local population.
8.3 Sample Collection
Fish collection methods can be divided into two major categories, active and passive. Each has
advantages and disadvantages. Active collection methods employ a wide variety of sampling devices
including electrofishing units, seines, trawls, and angling equipment (hook and line). Although active
collection requires greater fishing effort, it is usually more efficient than passive collection for covering a
large number of sites and catching the relatively small number of individuals needed from each site for
tissue analysis. The active collection methods generally require more field personnel and more
expensive equipment than passive collection methods. Passive collection methods employ a wide array
of sampling devices, including gill nets, fyke nets, trammel nets, hoop nets, pound nets, and d-traps.
Passive collection methods generally require less fishing effort than active methods, but normally yield a
much greater catch than would be required for a contaminant monitoring program. They are also time
consuming to deploy. Passive collection devices (e.g., gill nets) must be checked frequently (e.g., at
least once every 24 hours) to ensure a limited time lag between fish entrapment and sample
preparation/preservation.
Sampling Teams dedicated to the National Fish Tissue Study will be equipped with an array of both
active and passive gears to ensure the collection of the desired target numbers and species offish.
Selection of the most appropriate gear type(s) for a particular target lake will be at the discretion of the
experienced on-site fisheries biologist. USEPA Regional/State/Tribal Sampling Teams and Contractor-
affiliated Sampling Teams will be responsible for providing fisheries sampling gear and sampling vessels.
The sample control center will provide sample packaging and shipping supplies. A list of equipment
and expendable supplies is provided in Table 7. Sample collection, packaging, and shipment methods
are presented as Appendix B, Standard Operating Procedure.
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Table 7. Equipment and Supply List for Fish Tissue Sampling.
1. Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment}"'
2. Electrofishing equipment - OPTIONAL (including variable voltage pulsator unit, generator, electrodes, wiring cables, dip
nets, protective gloves, protective boots, and all necessary safety equipment}"'
3. Nets - OPTIONAL (including trawls, seines, gill nets, fyke nets, trammel nets, hoop nets, pound nets, trap nets)1"1
4. Angling equipment - OPTIONAL (including fishing rods, reels, line, terminal tackle, trot lines)1"'
5. Coast Guard-approved personal floatation devices
6. Maps of target lakes and access routes
7. Global Positioning System (GPS) unit - OPTIONAL <">
pH meter (including associated calibration supplies)("'
9. Livewell and/or buckets
10. Measuring board (millimeter scale)
11. Ice chests"'
12. Aluminum foil (solvent-rinsed and baked)*
13. Heavy-duty food grade polyethylene tubing*1
14. Large plastic (composite) bags(
15. Knife or scissors
16. Clean nitrile gloves *
17. Field Record Forms*'
18. Sample Identification Labels *'
19. Chain-of-Custody Forms *>
20. Chain-of-Custody Labels*
21. Scientific collection permit
22. Dry ice*
23. Black ballpoint pens and/or waterproof markers
24. Clipboard
25. Packing/strapping tape
26. Overnight courier airbills *'
27. Plastic cable ties*'
28. Plastic bubble-wrap*'
29. First aid kit and emergency telephone numbers
^ Selection and exact specifications at the discretion of the experienced on-site fisheries biologist.
(b) Provided by the sample control center.
As soon as fish are obtained via active collection methods, or removed from passive collection devices,
they should be identified to species. Species identification should be conducted only by experienced
personnel knowledgeable of the taxonomy of species in the waterbodies included in the fish
contaminant monitoring program. Nontarget species, collected by the field team should be returned to
the water. Individuals of the selected target species will be rinsed in ambient water to remove any
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foreign material from the external surface, should be handled using clean nitrile gloves (provided by the
sample control center), and placed in clean holding containers (livewell, buckets, etc.) to prevent
contamination. Each fish of the selected target species should be measured to determine total body
length (mm). Maximum body length should be measured, i.e., the length from the anterior-most part of
the fish to the tip of the longest caudal finray (when the lobes of the caudal fin are depressed
dorsoventrally). When sufficient numbers of the target species have been identified to make up a
suitable composite sample (i.e., five individuals meeting the size criteria presented in Section 8.2), the
species name, specimen lengths, and all other site and sampling information should be recorded on the
Field Record Form (Appendix B).
The field objective is for sampling teams to obtain a representative composite sample for both a
predator and a bottom-dwelling species from each lake or reservoir selected for the National Fish
Tissue Study. Each composite must consist of all the same species, individual fish must be of similar
size (i.e., all within 75% of the length of the largest fish), and the composite must be able to deliver 560
grams offish tissue (fillets for predators, and whole bodies for bottom-dwellers) for chemical analysis.
To obtain a representative sample of the targeted species in lakes and reservoirs (and particularly in
large waterbodies), field teams should consider factors such as habitat and presence of contaminant
gradients in planning sampling locations for the target lake. Ideally, the habitats suitable for target
species would be determined for the lake, and up to three locations of that habitat would be randomly
selected for sampling in the lake. If a contamination gradient may be present in the waterbody, then
three locations across the gradient should be selected for sampling. For example, in reservoirs, the
three locations may be in habitat near the inflow, middle, and outflow of the reservoir. The composite is
intended to estimate the mean fish tissue contaminant concentration for the lake or reservoir. Given the
diversity of lakes and reservoirs in the study, and given the multiple species that must be used, the study
must rely on the local knowledge of the field teams in the selection of the representative composite
sample.
9.0 SAMPLE HANDLING AND CUSTODY REQUIREMENTS
9.1 Sample Handling
Clean nitrile gloves (provided by the sample control center) should be worn during the entire sample
handling process, beginning with removing the fish from the sampling gear. Individuals of the selected
target species should be rinsed in ambient water to remove any foreign material from the external
surface. After initial processing to determine species and size, each of the five fish found to be suitable
for the composite sample will be individually wrapped in extra heavy-duty aluminum foil (provided by
the sample control center as solvent-rinsed, oven-baked sheets). For specimens with sharp fins, spines
may be broken (via gloved hands or with the use of a tool covered with the aluminum foil provided by
the sample control center) to prevent perforation of the wrapping materials. The broken section of the
fins should be included with the fish sample. A Sample Identification Label (Appendix B) will be
prepared for each aluminum foil-wrapped specimen. Each foil-wrapped fish will be placed into a
waterproof plastic tubing that will be cut to size to fit the specimen (i.e., heavy duty food grade
polyethylene tubing provided by the sample control center), and each end of the tubing will be sealed
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with a plastic cable tie. The completed Sample Identification Label will be affixed to the cable tie, and
the entire specimen package will be "double-bagged" (i.e., placed inside a large plastic bag with all the
specimens of the same species from that site and sealed with another cable tie). Once packaged,
samples should be immediately placed on dry ice for shipment. If samples will be carried back to a
laboratory or other facility to be frozen before shipment, wet ice can be used to transport wrapped and
bagged fish samples in the coolers to that laboratory or facility. If possible, all of the specimens in a
composite sample should be kept together in the same shipping container (ice chest) for transport.
Sampling Teams have the option, depending on site logistics, of:
shipping the samples packed on dry ice (in sufficient quantities to keep samples frozen
for up to 48 hours), via priority overnight delivery service (i.e., Federal Express), so
that they arrive at the sample preparation laboratory within less than 24 hours from the
time of sample collection, or
freezing the samples within 24 hours of collection (at <-20°C), and storing the frozen
samples until shipment within 1 week of sample collection (frozen samples will
subsequently be packed on dry ice and shipped to the sample preparation laboratory
via priority overnight delivery service to arrive within less than 24 hours from time of
shipment).
The time of sample collection, relinquishment by the sample team, and time of their arrival at the sample
preparation laboratory must be recorded on the Chain-of-Custody Form (Appendix B). Field
Sampling Teams should avoid shipping samples for weekend delivery to the sample preparation
laboratory unless prior plans for such a delivery have been agreed upon with the sample control center.
9.2 Sample Integrity
A critical requirement of the National Fish Tissue Study is the maintenance of sample integrity from the
time of collection to the shipment and arrival at the final destination. Sample integrity is maintained by
preventing the loss of contaminants that might be present in the sample and by taking precautions to
avoid possible introduction of contaminants during handling. The loss of contaminants can be prevented
in the field by ensuring that the sample collected remains intact, i.e., sample collection procedures
should be performed with the intention of minimizing the laceration offish skin. Once a sample is
collected, sample integrity is maintained through careful and controlled sample handling, storage, and
preservation procedures (Section 9.1).
Preventable sources of extraneous contamination can include the sampling gear, oils and greases on
boats, spilled fuel, skin contact, contact with soil or sand, boat motor exhaust, and other potential
sources. All potential sources should be identified before the onset and during sample collection, and
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appropriate measures
should be taken to minimize or eliminate them. Examples of preventative measures include the
following:
Collection nets should be free of any potential contaminants.
The use of tarred collection nets is prohibited.
Boats should be positioned so that engine exhaust does not fall on the deck area where
samples are being handled.
Ice chests and other sample storage containers should be scrubbed clean with detergent
and rinsed with distilled water prior to use (containers originating from the sample
control center will be prewashed and rinsed).
Samples should not be placed directly on dry ice, but should be stored inside foil,
plastic tubing (i.e., heavy-duty food grade polyethylene tubing as per Section 9.1), and
plastic garbage bags first.
Proper gloves (clean nitrile gloves) should be used when handling samples.
9.3 Custody Requirements
As soon as possible following collection, the Sampling Team will begin the process of identifying,
labeling, packaging, and storing the sample(s). Each sample will be identified and tracked with a unique
numbering scheme as described in Section 6.0. This ten-character composite code followed by a two-
digit specimen number will identify each sample on all documentation and records including the
following:
Field Record Form,
Sample Identification Label, and
Chain-of-Custody Form.
Each sample (i.e., individual fish) will be labeled by affixing a Sample Identification Label (Appendix B)
as per the instructions in Section 9.1. All sample label entries will be made with black indelible ink.
The sample label will accompany each sample throughout the chain-of-custody. Each sample label will
include the following information:
project name (USEPA National Fish Tissue Study),
site identification (lake name),
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sample number (01 through 05),
composite code (ten-digit code as in Section 6.0),
date of sample (month/day/year),
time of collection (military time),
preservative used (dry ice or frozen), and
collector's name (field team leader).
Detailed documentation of the samples collected in the field (for shipment to the sample preparation
laboratory) and information about the collection location will be recorded on a Field Record Form
(Appendix B). One form must be completed for each sample composite. One page of the four-page
carbonless copy form (Section 6.0) will be retained by the sampler, and the other copies will be
included with sample shipment to the sample preparation laboratory. (The sample preparation
laboratory will retain one copy, and be responsible for forwarding one copy to the sample control
center and one copy to the Tetra Tech Task Leader.) All entries will be made in black ink and no
erasures will be made. Each form will have the proper entry requirements, which includes the following
information:
composite code (ten digits as per Section 6.0),
sampling date (month/day/year),
time of collection (military time),
collection method (e.g., gill net),
collector's name (printed and signed),
collector's affiliation, address, and telephone number,
site name (lake name),
site description (location of lake and area of lake sampled),
lake type (e.g., natural lake),
estimated maximum depth (meters),
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fish species (common name),
length (mm) of each specimen,
location, date and time of collection for each specimen, and
a simple sketch of the sampling site and sample collection points.
All samples and composites will be transferred to the receiving laboratory (i.e., sample preparation
laboratory) under chain of custody. The Chain-of-Custody Form (Appendix B) acts as a record of
sample shipment and a catalog of the contents of each shipment (coinciding with information on the field
record). The forms will be produced as five-page carbonless copies with one copy retained by the
sampler and four for shipment to the laboratory (i.e., one for the sample preparation laboratory, one for
the sample control center, one for the Tetra Tech Task Leader, and one for duplication and distribution
to the analytical laboratories). The latter four copies will be placed in a waterproof plastic bag
(provided by the sample control center) and sealed inside the shipping container. All Chain-of-Custody
Form entries will be made in black ink and will include:
the USEPA Proj ect Manager's name, address and telephone number (refer to the
QAPP cover page),
sampler's name and telephone number,
project name (USEPA National Fish Tissue Study),
page number (e.g., 1 of 1),
sample location (lake name),
collection date and time,
composite code (ten-digit) and sample number (two-digit),
preservative (dry ice or frozen),
number of containers,
type of analysis required (USEPA 274 PBT target analytes [including breakdown
products and PCB congeners]),
sampler's signature, sample date, and time,
sampler relinquishment date and time,
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laboratory recipient signature, and
laboratory receipt date and time.
Immediately following the packing of each shipping container (Section 9.1), each container (ice chest)
will be secured with packaging tape and sealed with a Chain-of-Custody Label (provided by the
laboratory). The Chain-of-Custody Label must contain the signature of the sampler and the date and
time written in ink. The seal must be affixed such that the shipping container cannot be opened without
breaking the seal (e.g., label adhered across the ice chest latch), so as to protect and document the
integrity of the contents from field to laboratory.
10.0 ANALYTICAL METHODS REQUIREMENTS
Samples will be shipped (Section 9.1) under chain of custody to locations designated by the USEPA
Project Manager for processing and analytical testing. Sample processing and analytical testing and
methods are outside the scope of this QAPP and therefore are not addressed herein, but will be
discussed in the Analytical Activities QAPP.
11.0 QUALITY CONTROL REQUIREMENTS
Data quality is addressed, in part, by consistent performance of valid procedures documented in the
standard operating procedures (Appendix B). It is enhanced by the training and experience of project
staff (Section 5.0) and documentation of project activities (Section 6.0). This QAPP, a field sampling
plan, and training materials will be distributed to all USEPA/Regional/State/ Tribal Fish Sampling
Coordinators, and, in turn, to sampling personnel. Orientation sessions will be set up by EPA Regions
to distribute and discuss project materials (Section 5.0). USEPA Regional/State/Tribal Fish Sampling
Coordinators and Field Team Leaders will be required to view the training materials, read the QAPP,
and verify in writing that they read or viewed the materials and understood the procedures and
requirements.
12.0 INSTRUMENT/EQUIPMENT TESTING, INSPECTION, AND MAINTENANCE
REQUIREMENTS
All field equipment will be inspected prior to sampling activities to ensure that proper use requirements
are met (e.g., boats or electrofishers are operating correctly, nets are without defects, pH meter
properly calibrated). Inspection of field equipment will occur well in advance of the field operation to
allow time for replacement or repair of defective equipment, and the field team will be equipped with
proper backup equipment to prevent lost time on site. One member of each field team should gather
and inspect all equipment on the equipment and supply list (Table 7) prior to each sampling event.
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 31 of 36
13.0 INSTRUMENT CALIBRATION AND FREQUENCY
All pH meters used by field teams will be calibrated according to the manufacturer's operating
instructions, on a daily basis, while in use (Appendix C).
14.0 INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPLIES AND
CONSUMABLES
Careful and thorough planning is necessary to ensure the efficient and effective completion of the field
sample collection task. A general checklist of field equipment and supplies is provided in Table 7.
Sampling gear will be provided by each field team, and most sample preparation and shipment supplies
will be provided by the sample control center. It will be the responsibility of each field team to gather
and inspect the necessary sampling gear prior to the sampling event and to inspect the sample
packaging and shipping supplies received from the laboratory. Defective packaging and shipping
supplies (e.g., torn or damaged polyethylene sample tubing) will be discarded, and, if necessary, the
field team will contact the sample control center to obtain replacement supplies.
15.0 DATA ACQUISITION REQUIREMENTS (NONDIRECT MEASUREMENTS)
Nondirect measurements will include identification and/or verification of each sample lake location (i.e.,
latitude and longitude). Coordinates of the target lakes are provided in Appendix A as decimal degrees
and conventional degrees, minutes, and seconds. USEPA Regional/State/ Tribal Fish Sampling
Coordinators or Field Sampling Teams having corrections to the Appendix A coordinates for a
particular target lake (based on USGS quadrangle, or equivalent, map verification), need to report
those corrections to the USEPA Project Manager (telephone 202/260-7055) or Tetra Tech Task
Leader (telephone 410/356-8993).
16.0 DATA MANAGEMENT
Samples will be documented and tracked via Sample Identification Labels, Field Record Forms, and
Chain-of-Custody Forms (Section 6.0). Since the sampling effort is a cooperative one involving many
different partner agencies and groups, the diligence of the Field Sampling Teams in completion of the
proper records is essential. Field team leaders will be responsible for reviewing all completed field
forms. Any corrections should be noted, initialed, and dated by the reviewer (Section 6.0). As
mentioned in Section 6.0, Field Record Forms and Chain-of-Custody Forms will each be prepared
and replicated in the field, via multiple page "carbonless copy" forms. The sampler will retain one copy
each of the Field Record and Chain-of-Custody Forms, and the remaining copies will be delivered to
the sample preparation laboratory with the samples. Shipment of samples to the sample preparation
laboratory (Section 9.1) must be conducted by a delivery service that provides constant tracking of
shipments (e.g., Federal Express). Laboratory sample log-in and data management procedures are
beyond the scope of this QAPP.
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 32 of 36
The sample preparation laboratory will retain one copy of each Field Record Form and Chain-of-
Custody Form, and will forward a copy of each to the Tetra Tech Task Leader. All form copies
obtained by Tetra Tech will be maintained in a project file during the active phase of the project, and
for a period of 2 years following completion of the project (unless otherwise directed by USEPA).
Upon completion of annual sampling activities, Tetra Tech will develop a field collection effort summary
(i.e., a detailed listing of all sampling participants, sampling locations, and specimens collected) based
on information recorded by all Sampling Teams on the Field Record Forms. The Field Record Form
data will be entered into an ExcelŽ spreadsheet to create the annual summary. All data entries will be
checked for errors in transcription and computer input by a minimum of two persons. If there is any
indication that requirements for sample integrity or data quality have not been met, the Tetra Tech QA
Officer will be notified immediately (with an accompanying explanation of the problems encountered).
All computer files associated with the project will be stored in a project subdirectory by Tetra Tech,
and will be copied to disk for archive for the two years subsequent to project completion (unless
otherwise directed by the USEPA Project Manager).
C. ASSESSMENT/OVERSIGHT
17.0 ASSESSMENT AND RESPONSE ACTIONS
Assessment activities and corrective response actions have been identified to ensure that sample
collection activities are conducted as prescribed and that the measurement quality objectives and data
quality objectives established by USEPA are met. The QA program under which this project will
operate includes performance and system audits with independent checks of the data obtained from
sampling activities. Either type of audit could indicate the need for corrective action. The essential
steps in the program are as follows:
identify and define the problem,
assign responsibility for investigating the problem,
investigate and determine the cause of the problem,
assign and accept responsibility for implementing appropriate corrective action,
establish effectiveness of and implement the corrective action, and
verify that the corrective action has eliminated the problem.
Immediate corrective actions form part of normal operating procedures and are noted on project Field
Record Forms. Problems not solved this way require more formalized, long-term
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 33 of 36
corrective action. In the event that quality problems requiring attention are identified, the Tetra
Tech Task Leader and/or Tetra Tech QA Officer will determine whether attainment of acceptable data
quality requires either short- or long-term actions. Failure in an analytical system (e.g., performance
requirements are not met) and corrective actions for those failures are beyond the scope of this QAPP.
Communication and oversight will proceed from Field Sampling Team Leaders (e.g., senior fisheries
biologist) to the Tetra Tech Task Leader and the USEPA Regional/State/Tribal Fish Sampling
Coordinators. The Tetra Tech Task Leader will be on-call throughout the entire sampling period
(Section 7.2) to address questions and receive communications of sampling status from the Field
Sampling Teams. The Tetra Tech Task Leader will communicate the status of the sampling activities to
the USEPA Project Manager on a weekly basis (at a minimum). The USEPA Regional/State/Tribal
Fish Sampling Coordinators and Tetra Tech Task Leader will immediately consult with the Tetra Tech
QA Officer and USEPA Project Manager regarding any difficulties encountered during sample
collection activities. The Tetra Tech QA Officer will initiate the corrective action system described
above, documenting the nature of the problem and ensuring that the recommended corrective action is
carried out.
The USEPA Project Manager and/or the Tetra Tech QA Officer will work with the USEPA Regional/
State/Tribal Fish Sampling Coordinators and Tetra Tech Task Leader to determine the best way to
rectify the problem and obtain accurate and useable data. When corrective actions have been taken
and a sufficient time period has elapsed that allows a response, the response will be compared with
project goals by the USEPA Project Manager. The Tetra Tech QA Officer will verify that the
corrective action has been appropriately addressed to eliminate the problem. The USEPA QA
Manager has the authority to stop work on the project if problems affecting data quality are identified
that will require extensive effort to resolve. The USEPA Project Manager will consult with the USEPA
QA Manager regarding any and all corrective actions and stop work orders.
Performance audits are qualitative checks on different segments of project activities, and are most
appropriate for sampling, analysis, and data processing activities. Field audits will be conducted
periodically in accordance with Agency requirements and availability of resources. Performance audit
techniques include checks on sampling equipment, measurements, and the analysis of data quality using
QC and spiked samples. Analytical performance audits are beyond the scope of this QAPP. The
USEPA Regional/State/Tribal Fish Sampling Coordinators and/or the Tetra Tech Task Leader will be
responsible for overseeing work as it is performed, and periodically conducting QC checks during the
sample collection phase of this project.
System audits are qualitative reviews of project activity to check that the overall quality program is
functioning and that the appropriate QC measures identified in the QAPP are being implemented. The
Tetra Tech QA Officer will conduct one internal system audit during the project and report the results
to the USEPA Project Manager on Tetra Tech's standard Audit Report Form.
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 34 of 36
18.0 REPORTS TO MANAGEMENT
Following completion of the system audit, the Tetra Tech QA Officer will prepare an Audit Report
Form and submit copies to both the USEPA Project Manager and the USEPA QA Officer.
Upon completion of weekly sampling activities, the Tetra Tech Task Leader will contact the USEPA
Project Manager to summarize Field Sampling Team progress for the preceding week and submit a
weekly progress report detailing the sampling activities. Following completion of annual field sampling
activities, Tetra Tech will prepare an annual field collection effort summary (i.e., detailed listing of all
sampling participants, sampling locations, and specimens collected) for review by the USEPA Project
Manager. Following incorporation of USEPA Project Manager comments and final approval, the
summary will be used to report back to USEPA Regional/State/Tribal participants to document
collective sampling progress for each study year.
D. DATA VALIDATION AND USABILITY
19.0 DATA REVIEW, VALIDATION, AND VERIFICATION REQUIREMENTS
Data validation and review services provide a method for determining the usability and limitations of
data, and provide a standardized data quality assessment. All Field Record Forms and Chain-of-
Custody records will be reviewed by the Tetra Tech Task Leader (assisted by the QA Officer, as
needed) for completeness and correctness. Tetra Tech will be responsible for reviewing data entries
and transmittals for completeness and adherence to QA requirements. Data quality will be assessed by
comparing entered data to original data or by comparing results with the measurement performance
criteria summarized in Section 4.2 to determine whether to accept, reject, or qualify the data. Results
of the review and validation processes will be reported to the USEPA Project Manager.
20.0 VALIDATION AND VERIFICATION METHODS
All Field Record Forms and Chain-of-Custody records will be reviewed by the Tetra Tech Task
Leader. The Tetra Tech QA Officer will review a minimum of five percent of the Field Record Forms
and Chain-of-Custody records. Any discrepancies in the records will be reconciled with the
appropriate associated field personnel and will be reported to the USEPA Project Manager.
Analytical validation and verification methods are outside of the scope of this QAPP. The submission
of samples to the sample preparation laboratory will include Field Record Forms and Chain-of-
Custody Forms documenting sampling time and date. This information will be checked by the receiving
laboratory to ensure that holding times (Section 9.1) have not been exceeded. Violations of holding
times will be reported (by the laboratory) to the USEPA Project Manager and the Tetra Tech Task
Leader via the USEPA Sample Analysis Manager, and the
USEPA Project Manager will discuss with the USEPA Sample Analysis Manager whether or not to
issue a stop work order for analysis of that particular sample.
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National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 35 of 36
21.0 RECONCILIATION WITH DATA QUALITY OBJECTIVES
As soon as possible following completion of the sample collection task, precision, accuracy, and
completeness measures will be assessed by Tetra Tech and compared with the criteria discussed in
Section 4.0. This will represent the final determination of whether the data collected are of the correct
type, quantity, and quality to support their intended use for this project. Any problems encountered in
meeting the performance criteria (or uncertainties and limitations in the use of the data) will be discussed
with the USEPA Project Manager, and will be reconciled, if possible.
-------
National Study of Chemical Residues in Lake Fish Tissue QAPP Final
Date: 22 May 2000
Page 36 of 36
LITERATURE CITED
NOAA. 1993. Sampling and Analytical Methods of the National Status and Trends Program
National Benthic Surveillance and Mussel Watch Projects 1984-1992, Volumes I-IV.
National Oceanic and Atmospheric Administration, Coastal Monitoring and Bioeffects
Assessment Division, Office of Ocean Resources Conservation and Assessment, National
Ocean Service, Silver Spring, MD. NOAA Technical Memorandum NOS ORCA 71.
Reference No. 024.
Olsen, A.R., D.L. Stevens, Jr., and D. White. 1998. Application of global grids in environmental
sampling. Computing Science and Statistics. 30:279-284.
U.S. Environmental Protection Agency (USEPA). 1992. National Study of Chemical Residues
in Fish. U.S. Environmental Protection Agency, Office of Science and Technology,
Washington, D.C. EPA 823-R-92-008.
U.S. Environmental Protection Agency (USEPA). 1995. Guidance for Assessing Chemical
Contaminant Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second
Edition. U.S. Environmental Protection Agency, Office of Water, Washington, D.C. EPA
823-R-95-007.
U.S. Environmental Protection Agency (USEPA). 1999a. EPA Requirements for Quality Assurance
Project Plans. U.S. Environmental Protection Agency, Quality Assurance Division,
Washington, D.C. Interim Final. EPA/QA/R-5.
U.S. Environmental Protection Agency (USEPA). 1999b. National Study of Chemical Residues in
Lake Fish Tissue: Study Design. U.S. Environmental Protection Agency, Office of Science and
Technology, Washington, D.C.
U.S. Environmental Protection Agency (USEPA). 1999c. Update: Listing of Fish and Wildlife
Advisories. Fact Sheet. U.S. Environmental Protection Agency, Office of Water, Washington,
D.C. EPA 823-F-99-005.
-------
Appendix A
Randomly Selected List
of Target Lakes
-------
-------
OWOW99 Site-ID
OWOW99-0022
OWOW99-0072
OWOW99-0136
OWOW99-0147
OWOW99-0161
OWOW99-0197
OWOW99-0486
OWOW99-0511
OWOW99-0547
OWOW99-0560
OWOW99-0622
OWOW99-0647
OWOW99-0923
OWOW99-0947
OWOW99-0961
OWOW99-1072
OWOW99-1436
OWOW99-1472
OWOW99-1497
OWOW99-0047
OWOW99-0097
OWOW99-0122
OWOW99-0143
OWOW99-0171
OWOW99-0222
OWOW99-0497
Long. DD
-87.3315
-85.0969
-87.1320
-86.0285
-87.0398
-87.3823
-86.3385
-85.5705
-87.1202
-85.1396
-85.3245
-85.7285
-87.2961
-87.4428
-86.2978
-86.7524
-85.9938
-86.0533
-86.3959
-93.9120
-91.9745
-91.3425
-92.2420
-91.7495
-90.3498
-93.8325
Lat. DD
33.9487
31.9344
34.0809
31.1530
34.6639
32.0983
33.3199
34.0993
33.3243
32.4487
31.1539
32.4403
31.4475
32.8863
34.1229
32.3890
33.6132
32.3590
33.1694
35.2279
34.9647
35.7756
36.4063
34.1479
34.8895
35.5319
Lake Name
WALTER F GEORGE
RES
Lewis Smith Lake
Wheeler Lake
Clark's lake
Pine Lake
Kelley Lake
Payne Lake
Jones Bluff Lake
Candles Lake
NORFOLK L
St.
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AL
AR
AR
AR
AR
AR
AR
AR
County
WALKER
BARBOUR
WINSTON
GENEVA
WILCOX
TALLADEGA
CHEROKEE
JEFFERSON
RUSSELL
HOUSTON
MACON
MONROE
HALE
MARSHALL
LOWNDES
CALHOUN
MONTGOMERY
TALLADEGA
LOGAN
LONOKE
INDEPENDENCE
BAXTER
JEFFERSON
CRITTENDEN
FRANKLIN
Lake
Area
(ha)
4
15282
8793
6
27143
4738
16
48
7
3
3
3
2
46
3
5063
7
1
26
6
3
2
7546
5
2
166
Yr.
1
1
1
1
1
1
2
2
2
2
2
2
3
3
3
3
4
4
4
1
1
1
1
1
1
2
Longitude
Deg
87
85
87
86
87
87
86
85
87
85
85
85
87
87
86
86
85
86
86
93
91
91
92
91
90
93
Min
19
5
7
1
2
22
20
34
7
8
19
43
17
26
17
45
59
3
23
54
58
20
14
44
20
49
Sec
53.40
48.84
55.20
42.60
23.21
56.28
18.60
13.80
12.72
22.56
28.20
42.60
45.96
34.08
52.08
8.64
37.68
11.88
45.24
43.20
28.20
33.00
31.20
58.20
59.28
57.00
Latitude
Deg
33
31
34
31
34
32
33
34
33
32
31
32
31
32
34
32
33
32
33
35
34
35
36
34
34
35
Min
56
56
4
9
39
5
19
5
19
26
9
26
26
53
7
23
36
21
10
13
57
46
24
8
53
31
Sec
55.32
3.84
51.24
10.80
49.93
53.88
11.64
57.48
27.48
55.32
14.04
25.08
51.00
10.68
22.44
20.40
47.52
32.40
9.84
40.44
52.92
32.16
22.68
52.44
22.20
54.84
1 of 35
-------
OWOW99 Site-ID
OWOW99-0523
OWOW99-0571
OWOW99-0623
OWOW99-0922
OWOW99-0971
OWOW99-1022
OWOW99-1046
OWOW99-1371
OWOW99-1396
OWOW99-1398
OWOW99-1447
OWOW99-1449
OWOW99-1493
OWOW99-1522
OWOW99-0045
OWOW99-0569
OWOW99-0595
OWOW99-1020
OWOW99-1044
OWOW99-1520
OWOW99-0001
OWOW99-0002
OWOW99-0018
OWOW99-0026
OWOW99-0027
OWOW99-0051
Long. DD
-93.7021
-92.1631
-93.1037
-91.7543
-91.0572
-90.7207
-92.5217
-93.3895
-91.3933
-94.0040
-91.6630
-93.2374
-93.9496
-90.3370
-111.2923
-109.5274
-110.0558
-114.6362
-109.4212
-114.3657
-120.4193
-119.2349
-114.5414
-123.7864
-119.7812
-122.1163
Lat. DD
33.2410
35.5610
34.1847
34.7737
34.2542
34.7570
34.5720
34.6169
34.4663
33.7506
34.9565
34.2571
36.3670
34.9306
33.5876
34.1152
34.3240
35.4539
33.9097
34.5009
41.9579
36.8747
33.5724
40.0640
38.1627
37.7861
Lake Name
LOUCHITA
Millwood Lake
Degray Lake
BEAVER RES
Aoache Lake
Carnero Lake
White Lake
L MOJAVE
Crescent Lake
L HAVASU
Goose Lake
St.
AR
AR
AR
AR
AR
AR
AR
AR
AR
AR
AR
AR
AR
AR
AZ
AZ
AZ
AZ
AZ
AZ
CA
CA
CA
CA
CA
CA
County
LAFAYETTE
CLEBURNE
CLARK
LONOKE
PHILLIPS
LEE
SALINE
GARLAND
ARKANSAS
LITTLE RIVER
PRAIRIE
CLARK
BENTON
CRITTENDEN
MARICOPA
APACHE
NAVAJO
MOHAVE
APACHE
MOHAVE
FRESNO
RIVERSIDE
HUMBOLDT
TUOLUMNE
ALAMEDA
Lake
Area
(ha)
21
4803
152
7
2
1
4
15816
24
9668
6
4576
8311
872
888
27
10
10446
64
7223
0
2337
7
25
3
309
Yr.
2
2
2
3
3
3
3
4
4
4
4
4
4
4
1
2
2
3
3
4
1
1
1
1
1
1
Longitude
Deg
93
92
93
91
91
90
92
93
91
94
91
93
93
90
111
109
110
114
109
114
120
119
114
123
119
122
Min
42
9
6
45
3
43
31
23
23
0
39
14
56
20
17
31
3
38
25
21
25
14
32
47
46
6
Sec
7.56
47.16
13.32
15.48
25.92
14.52
18.12
22.20
35.88
14.40
46.80
14.64
58.56
13.20
32.28
38.64
20.88
10.32
16.32
56.52
9.59
5.64
29.04
11.04
52.32
58.68
Latitude
Deg
33
35
34
34
34
34
34
34
34
33
34
34
36
34
33
34
34
35
33
34
41
36
33
40
38
37
Min
14
33
11
46
15
45
34
37
27
45
57
15
22
55
35
6
19
27
54
30
57
52
34
3
9
47
Sec
27.60
39.60
4.92
25.32
15.12
25.20
19.20
0.84
58.68
2.16
23.40
25.56
1.20
50.16
15.36
54.72
26.40
14.04
34.92
3.24
28.58
28.92
20.64
50.40
45.72
9.96
2 of 35
-------
OWOW99 Site-ID
OWOW99-0077
OWOW99-0118
OWOW99-0126
OWOW99-0128
OWOW99-0151
OWOW99-0201
OWOW99-0452
OWOW99-0468
OWOW99-0476
OWOW99-0477
OWOW99-0503
OWOW99-0551
OWOW99-0577
OWOW99-0601
OWOW99-0603
OWOW99-0651
OWOW99-0953
OWOW99-0977
OWOW99-1002
OWOW99-1026
OWOW99-1076
OWOW99-1118
OWOW99-1351
OWOW99-1378
OWOW99-1402
OWOW99-1418
Long. DD
-120.4489
-116.8157
-122.7705
-118.1459
-121.3599
-122.1881
-118.6846
-116.7810
-122.3975
-119.0280
-121.1275
-120.5188
-120.8588
-121.1105
-118.1823
-121.1289
-119.4818
-118.9776
-120.8496
-120.1551
-121.8182
-115.8314
-120.4928
-120.8460
-117.9516
-118.0490
Lat. DD
40.5446
34.0550
39.0266
35.0290
39.5799
41.4196
37.1583
32.9124
40.8253
38.0050
37.0439
40.2272
37.1225
38.4797
34.2971
41.5180
36.0562
37.3797
37.8529
38.9911
38.0663
33.3079
39.4114
35.3417
36.1876
33.6996
Lake Name
Clear Lake
Lake Oroville
SHASTA L
Mono Lake
San Luis Res
Salton Sea
St.
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
CA
County
LASSEN
SAN BERNARDINO
LAKE
KERN
BUTTE
SISKIYOU
FRESNO
SAN DIEGO
SHASTA
MONO
MERCED
PLUMAS
MERCED
SACRAMENTO
LOS ANGELES
MODOC
TULARE
FRESNO
STANISLAUS
EL DORADO
SOLANO
IMPERIAL
NEVADA
SAN LUIS OBISPO
INYO
ORANGE
Lake
Area
(ha)
11
74
15956
1
1730
294
2
590
5468
16302
5214
2
6
2
33
55
44
755
719
8
476
94543
89
858
687
6
Yr.
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
3
3
3
3
3
3
4
4
4
4
Longitude
Deg
120
116
122
118
121
122
118
116
122
119
121
120
120
121
118
121
119
118
120
120
121
115
120
120
117
118
Min
26
48
46
8
21
11
41
46
23
1
7
31
51
6
10
7
28
58
50
9
49
49
29
50
57
2
Sec
56.04
56.52
13.80
45.24
35.64
17.16
4.56
51.60
51.00
40.80
39.00
7.68
31.68
37.80
56.28
44.04
54.48
39.36
58.56
18.36
5.52
53.04
34.08
45.60
5.76
56.40
Latitude
Deg
40
34
39
35
39
41
37
32
40
38
37
40
37
38
34
41
36
37
37
38
38
33
39
35
36
33
Min
32
3
1
1
34
25
9
54
49
0
2
13
7
28
17
31
3
22
51
59
3
18
24
20
11
41
Sec
40.56
18.00
35.76
44.40
47.64
10.56
29.88
44.64
31.08
18.00
38.04
37.92
21.00
46.92
49.56
4.80
22.32
46.92
10.44
27.96
58.68
28.44
41.04
30.12
15.36
58.56
3 of 35
-------
OWOW99 Site-ID
OWOW99-1426
OWOW99-1427
OWOW99-1502
OWOW99-1526
OWOW99-1528
OWOW99-1551
OWOW99-0019
OWOW99-0028
OWOW99-0078
OWOW99-0176
OWOW99-0469
OWOW99-0478
OWOW99-0552
OWOW99-0903
OWOW99-0969
OWOW99-0994
OWOW99-1003
OWOW99-1394
OWOW99-1428
OWOW99-1569
OWOW99-0117
OWOW99-0938
OWOW99-1117
OWOW99-1538
OWOW99-0025
OWOW99-0050
Long. DD
-122.7696
-119.4148
-121.9981
-120.7485
-120.4040
-120.1479
-102.3823
-104.7065
-107.3337
-108.0405
-102.2763
-105.0226
-106.2063
-104.4631
-106.5172
-103.7030
-105.0564
-107.8886
-105.3511
-104.8543
-71.9430
-73.4959
-72.9549
-72.7665
-81.8469
-81.9264
Lat. DD
40.8951
37.6232
37.4396
38.7982
34.9692
41.7342
37.4862
40.0127
39.7020
39.0011
40.0854
40.5558
40.0177
40.8024
37.4731
38.1887
40.7888
37.8946
40.8820
39.6397
42.0115
41.3409
41.9704
41.5074
28.4754
28.6951
Lake Name
Claire Engle
Upper Alkali Lake (dry)
Quinebaug River
Rainbow Lake
Barkhamsted Reservoir
Foster Lake
St.
CA
CA
CA
CA
CA
CA
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CT
CT
CT
CT
FL
FL
County
TRINITY
MADERA
SANTA CLARA
EL DORADO
SANTA BARBARA
MODOC
BACA
WELD
GARFIELD
DELTA
YUMA
LARIMER
GRAND
WELD
RIO GRANDE
CROWLEY
LARIMER
SAN MIGUEL
LARIMER
ARAPAHOE
WINDHAM
FAIRFIELD
LITCHFIELD
NEW HAVEN
LAKE
LAKE
Lake
Area
(ha)
6757
4
61
9
941
11196
22
6
9
6
7
3
546
1
6
2239
10
2
108
347
11
15
891
4
78
10
Yr.
4
4
4
4
4
4
1
1
1
1
2
2
2
3
3
3
3
4
4
4
1
3
3
4
1
1
Longitude
Deg
122
119
121
120
120
120
102
104
107
108
102
105
106
104
106
103
105
107
105
104
71
73
72
72
81
81
Min
46
24
59
44
24
8
22
42
20
2
16
1
12
27
31
42
3
53
21
51
56
29
57
45
50
55
Sec
10.56
53.28
53.16
54.60
14.40
52.44
56.28
23.40
1.32
25.80
34.68
21.36
22.68
47.16
1.92
10.80
23.04
18.96
3.96
15.48
34.80
45.24
17.64
59.40
48.84
35.04
Latitude
Deg
40
37
37
38
34
41
37
40
39
39
40
40
40
40
37
38
40
37
40
39
42
41
41
41
28
28
Min
53
37
26
47
58
44
29
0
42
0
5
33
1
48
28
11
47
53
52
38
0
20
58
30
28
41
Sec
42.36
23.52
22.56
53.52
9.12
3.12
10.32
45.72
7.20
3.96
7.44
20.88
3.72
8.64
23.16
19.32
19.68
40.56
55.20
22.92
41.40
27.24
13.44
26.64
31.44
42.36
4 of 35
-------
OWOW99 Site-ID
OWOW99-0060
OWOW99-0075
OWOW99-0100
OWOW99-0125
OWOW99-0135
OWOW99-0150
OWOW99-0160
OWOW99-0175
OWOW99-0200
OWOW99-0225
OWOW99-0475
OWOW99-0498
OWOW99-0500
OWOW99-0510
OWOW99-0525
OWOW99-0535
OWOW99-0550
OWOW99-0574
OWOW99-0600
OWOW99-0610
OWOW99-0625
OWOW99-0650
OWOW99-0675
OWOW99-0925
OWOW99-0960
OWOW99-0972
Long. DD
-82.3394
-81.5695
-82.3507
-80.9404
-81.8437
-80.7960
-80.5882
-82.2463
-84.9271
-82.2637
-81.6036
-86.3279
-81.6221
-80.9802
-81.1501
-81.2028
-80.6610
-81.9198
-82.2659
-81.6416
-80.2610
-82.5276
-81.8420
-81.8724
-81.4955
-85.4548
Lat. DD
30.0367
24.7544
28.9242
27.9389
29.1804
27.1752
27.7803
27.5928
30.6812
28.3406
28.5265
30.4826
28.6191
28.2704
26.4247
29.5902
24.9913
29.0737
27.9661
29.4031
26.0262
29.4948
27.8500
28.9253
28.6981
30.7352
Lake Name
Tsala Apopka Lake
Lake Okeechobee
Lake Apopka
St.
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
County
UNION
MONROE
OSCEOLA
MARION
OKEECHOBEE
INDIAN RIVER
MANATEE
JACKSON
PASCO
ORANGE
WALTON
ORANGE
OSCEOLA
HENDRY
FLAGLER
MONROE
MARION
HILLSBOROUGH
PUTNAM
BROWARD
ALACHUA
POLK
LAKE
ORANGE
WASHINGTON
Lake
Area
(ha)
363
24
0
2
140
4830
3
2
3
62
76
2
12439
5
24
111
53
7
22
3
5
7
130
9
23
5
Yr.
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
3
3
3
Longitude
Deg
82
81
82
80
81
80
80
82
84
82
81
86
81
80
81
81
80
81
82
81
80
82
81
81
81
85
Min
20
34
21
56
50
47
35
14
55
15
36
19
37
58
9
12
39
55
15
38
15
31
50
52
29
27
Sec
21.84
10.20
2.52
25.44
37.32
45.60
17.52
46.68
37.56
49.32
12.96
40.44
19.56
48.72
0.36
10.08
39.60
11.28
57.24
29.76
39.60
39.36
31.20
20.64
43.80
17.28
Latitude
Deg
30
24
28
27
29
27
27
27
30
28
28
30
28
28
26
29
24
29
27
29
26
29
27
28
28
30
Min
2
45
55
56
10
10
46
35
40
20
31
28
37
16
25
35
59
4
57
24
1
29
51
55
41
44
Sec
12.12
15.84
27.23
20.04
49.44
30.72
49.08
34.08
52.32
26.16
35.40
57.36
8.76
13.44
28.92
24.72
28.68
25.32
57.96
11.16
34.32
41.28
0.00
31.08
53.16
6.72
5 of 35
-------
OWOW99 Site-ID
OWOW99-0975
OWOW99-1000
OWOW99-1025
OWOW99-1050
OWOW99-1060
OWOW99-1075
OWOW99-1100
OWOW99-1125
OWOW99-1385
OWOW99-1400
OWOW99-1425
OWOW99-1450
OWOW99-1475
OWOW99-1485
OWOW99-1500
OWOW99-1525
OWOW99-1535
OWOW99-1550
OWOW99-1575
OWOW99-0011
OWOW99-0036
OWOW99-0061
OWOW99-0086
OWOW99-0111
OWOW99-0185
OWOW99-0186
Long. DD
-81.4995
-81.3723
-82.6637
-82.3075
-81.8922
-85.7628
-82.3138
-81.0849
-81.4253
-81.8260
-81.4320
-81.7713
-83.5613
-82.1732
-80.9613
-81.9134
-80.8242
-81.8328
-81.7677
-82.0992
-84.6784
-83.8010
-85.1335
-83.2689
-82.4576
-82.7802
Lat. DD
27.7380
28.2325
28.2997
27.4795
29.6284
30.4994
26.8617
28.1410
28.9124
26.8045
28.1606
28.8391
30.0024
29.4535
25.3195
27.7037
28.9187
26.3086
27.9878
32.5078
31.9560
34.6489
33.0623
31.5345
31.0359
34.0842
Lake Name
L TOHOPEKALIGA
Orange Lake
West Point Lake
St.
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
FL
GA
GA
GA
GA
GA
GA
GA
County
POLK
OSCEOLA
PASCO
MANATEE
PUTNAM
WASHINGTON
CHARLOTTE
OSCEOLA
LAKE
CHARLOTTE
OSCEOLA
LAKE
TAYLOR
MONROE
POLK
VOLUSIA
COLLIER
POLK
CANDLER
STEWART
WHITE
TROUP
IRWIN
WARE
ELBERT
Lake
Area
(ha)
1400
7643
8
593
18
20
16
7
3
6
57
2
6
5142
50
113
4
13
259
8
1
16
9215
3
4
2
Yr.
3
3
3
3
3
3
3
3
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
Longitude
Deg
81
81
82
82
81
85
82
81
81
81
81
81
83
82
80
81
80
81
81
82
84
83
85
83
82
82
Min
29
22
39
18
53
45
18
5
25
49
25
46
33
10
57
54
49
49
46
5
40
48
8
16
27
46
Sec
58.20
20.28
49.32
27.00
31.92
46.08
49.68
5.64
31.08
33.60
55.20
16.68
40.68
23.66
40.68
48.24
27.12
58.08
3.72
57.12
42.24
3.60
0.60
8.04
27.36
48.72
Latitude
Deg
27
28
28
27
29
30
26
28
28
26
28
28
30
29
25
27
28
26
27
32
31
34
33
31
31
34
Min
44
13
17
28
37
29
51
8
54
48
9
50
0
27
19
42
55
18
59
30
57
38
3
32
2
5
Sec
16.80
57.00
58.92
46.20
42.24
57.84
42.12
27.60
44.64
16.20
38.16
20.76
8.64
12.46
10.20
13.32
7.32
30.96
16.08
28.08
21.60
56.04
44.28
4.20
9.24
3.12
6 of 35
-------
OWOW99 Site-ID
OWOW99-0461
OWOW99-0586
OWOW99-0636
OWOW99-0661
OWOW99-0911
OWOW99-0950
OWOW99-0986
OWOW99-1011
OWOW99-1035
OWOW99-1085
OWOW99-1097
OWOW99-1 1 1 1
OWOW99-1360
OWOW99-1386
OWOW99-1411
OWOW99-1461
OWOW99-1511
OWOW99-1547
OWOW99-1561
OWOW99-0082
OWOW99-0165
OWOW99-0540
OWOW99-0615
OWOW99-0907
OWOW99-0965
OWOW99-1040
Long. DD
-82.2751
-83.1705
-83.2274
-82.7082
-83.4105
-84.3761
-81.6357
-84.6197
-84.6319
-82.5690
-83.8326
-82.7243
-84.9227
-85.2008
-83.8231
-82.3983
-83.2477
-84.9136
-83.2858
-95.7467
-93.6948
-93.7902
-95.8102
-94.8276
-92.3738
-93.7316
Lat. DD
32.5185
31.6930
34.0646
32.8112
33.0359
30.6983
32.9502
32.3871
34.1368
31.0989
30.8728
31.2453
33.6541
34.2992
31.1853
33.6589
31.8803
30.7852
33.2307
43.3695
42.8389
40.8780
40.7771
43.4883
40.9740
41.7532
Lake Name
J Strom Thurmond Res
LSEMINOLE
Lake Sinclair
Morse Lake
Unnamed lake
Percival Lake
Eagle Lake
Sand pit
Saylorville Lake
St.
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
GA
IA
IA
IA
IA
IA
IA
IA
County
EMANUEL
BEN HILL
MADISON
WASHINGTON
JONES
GRADY
SCREVEN
MARION
CHEROKEE
WARE
THOMAS
ATKINSON
CARROLL
FLOYD
COLQUITT
COLUMBIA
WILCOX
SEMINOLE
PUTNAM
OSCEOLA
WRIGHT
DECATUR
FREMONT
EMMET
WAPELLO
POLK
Lake
Area
(ha)
6
3
33
13
1
13
2
10
4661
3
5
3
6
12
4
10307
40
5138
2071
6
41
3
6
82
13
2041
Yr.
2
2
2
2
3
3
3
3
3
3
3
3
4
4
4
4
4
4
4
1
1
2
2
3
3
3
Longitude
Deg
82
83
83
82
83
84
81
84
84
82
83
82
84
85
83
82
83
84
83
95
93
93
95
94
92
93
Min
16
10
13
42
24
22
38
37
37
34
49
43
55
12
49
23
14
54
17
44
41
47
48
49
22
43
Sec
30.36
13.80
38.64
29.52
37.80
33.96
8.52
10.92
54.84
8.40
57.36
27.48
21.72
2.88
23.16
53.88
51.72
48.96
8.88
48.12
41.28
24.72
36.72
39.36
25.68
53.76
Latitude
Deg
32
31
34
32
33
30
32
32
34
31
30
31
33
34
31
33
31
30
33
43
42
40
40
43
40
41
Min
31
41
3
48
2
41
57
23
8
5
52
14
39
17
11
39
52
47
13
22
50
52
46
29
58
45
Sec
6.60
34.80
52.56
40.32
9.24
53.88
0.72
13.56
12.48
56.04
22.08
43.08
14.76
57.12
7.08
32.04
49.08
6.72
50.52
10.20
20.04
40.80
37.56
17.88
26.40
11.52
7 of 35
-------
OWOW99 Site-ID
OWOW99-1090
OWOW99-1432
OWOW99-0079
OWOW99-0127
OWOW99-0177
OWOW99-0553
OWOW99-0554
OWOW99-0627
OWOW99-0904
OWOW99-1028
OWOW99-1452
OWOW99-0015
OWOW99-0041
OWOW99-0091
OWOW99-0115
OWOW99-0140
OWOW99-0190
OWOW99-0491
OWOW99-0515
OWOW99-0916
OWOW99-0990
OWOW99-1065
OWOW99-1115
OWOW99-1390
OWOW99-1415
OWOW99-1441
Long. DD
-94.2594
-94.8721
-117.0784
-111.1113
-112.9475
-114.6393
-116.8576
-111.3329
-115.9208
-115.8469
-111.5860
-88.7835
-88.6600
-89.2082
-89.8931
-90.6031
-89.3736
-87.5327
-89.5855
-89.3615
-89.8377
-88.9741
-90.2936
-89.9905
-90.0621
-88.2174
Lat. DD
41.5498
42.9460
44.6758
43.2436
42.7355
43.7787
48.5679
42.0037
45.0938
45.0996
42.9042
37.7733
41.6475
41.0500
39.4512
41.4549
38.5090
41.6645
40.5838
41.2039
38.2833
38.0812
40.1154
40.3401
38.9224
41.2945
Lake Name
Diamondhead Lake
Rush Lake
Brownlee Reservoir
PALISADES RES
Priest Lake
Bear Lake
Blackfoot Reservoir
Unnamed lake
Buck Lake
Unnamed lake
Otter Lake
Unnamed lake
Unnamed lake
Wolf Lake
Unnamed lake
Unnamed lake
Unnamed lake
Rend Lake
Crane Lake
McHarry Pond
Unnamed lake
Unnamed lake
St.
IA
IA
ID
ID
ID
ID
ID
ID
ID
ID
ID
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
IL
County
GUTHRIE
PALO ALTO
WASHINGTON
BONNEVILLE
POWER
ELAINE
BONNER
VALLEY
VALLEY
CARIBOU
WILLIAMSON
DEKALB
MARSHALL
MACOUPIN
ROCK ISLAND
CLINTON
COOK
TAZEWELL
PUTNAM
ST CLAIR
FRANKLIN
MASON
MASON
MADISON
WILL
Lake
Area
(ha)
40
178
6071
6062
6
8
9454
28329
3
3
6475
6
4
2
126
2
7
323
17
31
4
833
73
6
5
25
Yr.
3
4
1
1
1
2
2
2
3
3
4
1
1
1
1
1
1
2
2
3
3
3
3
4
4
4
Longitude
Deg
94
94
117
111
112
114
116
111
115
115
111
88
88
89
89
90
89
87
89
89
89
88
90
89
90
88
Min
15
52
4
6
56
38
51
19
55
50
35
47
39
12
53
36
22
31
35
21
50
58
17
59
3
13
Sec
33.84
19.56
42.35
40.68
50.86
21.44
27.50
58.48
14.81
48.88
9.67
0.60
36.00
29.52
35.16
11.16
24.96
57.72
7.80
41.40
15.72
26.76
36.96
25.80
43.56
2.64
Latitude
Deg
41
42
44
43
42
43
48
42
45
45
42
37
41
41
39
41
38
41
40
41
38
38
40
40
38
41
Min
32
56
40
14
44
46
34
0
5
5
54
46
38
2
27
27
30
39
35
12
16
4
6
20
55
17
Sec
59.28
45.60
32.74
36.96
7.94
43.39
4.37
13.32
37.50
58.45
15.01
23.88
51.00
60.00
4.32
17.64
32.40
52.20
1.68
14.04
59.88
52.32
55.44
24.36
20.64
40.20
8 of 35
-------
OWOW99 Site-ID
OWOW99-1465
OWOW99-1565
OWOW99-0141
OWOW99-0191
OWOW99-0216
OWOW99-0466
OWOW99-0590
OWOW99-0616
OWOW99-0940
OWOW99-0941
OWOW99-1416
OWOW99-1516
OWOW99-1541
OWOW99-0044
OWOW99-0119
OWOW99-0168
OWOW99-0218
OWOW99-0568
OWOW99-0619
OWOW99-0668
OWOW99-0943
OWOW99-0993
OWOW99-1019
OWOW99-1119
OWOW99-1368
OWOW99-1519
Long. DD
-88.5078
-89.4784
-86.7549
-85.5530
-86.3262
-85.8336
-87.5286
-85.9425
-87.2352
-85.6889
-86.3382
-85.0236
-86.9530
-97.7495
-96.7013
-94.7718
-95.8411
-95.9745
-98.4462
-96.2702
-96.5009
-98.0562
-99.2180
-95.6010
-95.1619
-98.2340
Lat. DD
37.7370
37.8187
39.7330
40.7298
39.0996
41.2229
39.0672
39.9282
39.0350
38.7726
41.6112
41.6268
40.0349
38.4030
39.4570
38.0787
38.2688
38.5312
37.9214
38.4313
37.3790
37.9161
39.5027
39.5024
38.5113
39.1122
Lake Name
Unnamed lake
Kinkaid Lake
Baire Lake
Winona Lake
Turtle Creek Reservoir
Geist Reservoir
Round Lake
Hardy Lake
(gravel pit)
Fox Lake
Unnamed lake
Tuttle Creek Lake
St.
IL
IL
IN
IN
IN
IN
IN
IN
IN
IN
IN
IN
IN
KS
KS
KS
KS
KS
KS
KS
KS
KS
KS
KS
KS
KS
County
SALINE
JACKSON
PUTNAM
HUNTINGTON
BROWN
KOSCIUSKO
SULLIVAN
HAMILTON
GREENE
SCOTT
ST JOSEPH
STEUBEN
MONTGOMERY
MCPHERSON
POTTAWATOMIE
LINN
COFFEY
LYON
RENO
LYON
ELK
RENO
ROOKS
JACKSON
FRANKLIN
LINCOLN
Lake
Area
(ha)
8
972
3
102
59
216
606
683
8
316
18
53
5
12
2153
2
3
2
6
1
2
2
2
5
10
3
Yr.
4
4
1
1
1
2
2
2
3
3
4
4
4
1
1
1
1
2
2
2
3
3
3
3
4
4
Longitude
Deg
88
89
86
85
86
85
87
85
87
85
86
85
86
97
96
94
95
95
98
96
96
98
99
95
95
98
Min
30
28
45
33
19
50
31
56
14
41
20
1
57
44
42
46
50
58
26
16
30
3
13
36
9
14
Sec
28.08
42.24
17.64
10.80
34.32
0.96
42.96
33.00
6.72
20.04
17.52
24.96
10.80
58.20
4.68
18.48
27.96
28.20
46.32
12.72
3.24
22.32
4.80
3.60
42.84
2.40
Latitude
Deg
37
37
39
40
39
41
39
39
39
38
41
41
40
38
39
38
38
38
37
38
37
37
39
39
38
39
Min
44
49
43
43
5
13
4
55
2
46
36
37
2
24
27
4
16
31
55
25
22
54
30
30
30
6
Sec
13.20
7.32
58.80
47.28
58.56
22.44
1.92
41.52
6.00
21.36
40.32
36.48
5.64
10.80
25.20
43.32
7.68
52.32
17.04
52.68
44.40
57.96
9.72
8.64
40.68
43.92
9 of 35
-------
OWOW99 Site-ID
OWOW99-1568
OWOW99-0037
OWOW99-0465
OWOW99-0640
OWOW99-0641
OWOW99-1012
OWOW99-1062
OWOW99-1361
OWOW99-0024
OWOW99-0074
OWOW99-0124
OWOW99-0149
OWOW99-0173
OWOW99-0174
OWOW99-0199
OWOW99-0224
OWOW99-0474
OWOW99-0549
OWOW99-0575
OWOW99-0599
OWOW99-0649
OWOW99-0674
OWOW99-0999
OWOW99-1048
OWOW99-1074
OWOW99-1099
Long. DD
-95.6120
-86.1933
-88.4942
-85.6473
-84.7146
-85.2710
-84.7791
-88.1218
-89.8980
-92.3439
-90.1274
-93.4958
-93.3868
-93.4135
-93.0480
-89.8645
-89.7188
-91.9919
-89.5451
-91.6058
-91.5507
-89.2912
-90.5717
-93.7341
-93.4158
-92.0605
Lat. DD
37.8871
37.3077
37.2822
37.7978
37.6850
37.2335
36.9740
37.0234
29.6683
31.5566
30.1925
32.1731
32.4381
30.8376
29.9418
30.6719
30.1291
31.6677
29.4416
30.7783
29.9396
29.9980
29.9208
32.3496
30.2565
32.5879
Lake Name
Unknown
Unknown
Lake Ponchartrain
Unknown
Lake Bisteneau
Unknown
Unknown
Unknown
Lake St. Catherine?
Unknown
Allen Bay
Unknown
Unknown
Indian Mound Bay?
Lac des Allemands
Unknown
Unknown
Unknown
St.
KS
KY
KY
KY
KY
KY
KY
KY
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
LA
County
WOODSON
EDMONSON
LIVINGSTON
NELSON
BOYLE
ADAIR
PULASKI
LYON
PLAQUEMINES
GRANT
RED RIVER
WEBSTER
BEAUREGARD
CAMERON
WASHINGTON
ORLEANS
CATAHOULA
PLAQUEMINES
POINTE COUPEE
ST MARY
ST BERNARD
CADDO
CALCASIEU
OUACHITA
Lake
Area
(ha)
2
808
13
3
1084
3191
231
8
4
11
#####
8
6283
5
602
34
3109
19
16
55
10
82
5957
8
64
6
Yr.
4
1
2
2
2
3
3
4
1
1
1
1
1
1
1
1
2
2
2
2
2
2
3
3
3
3
Longitude
Deg
95
86
88
85
84
85
84
88
89
92
90
93
93
93
93
89
89
91
89
91
91
89
90
93
93
92
Min
36
11
29
38
42
16
46
7
53
20
7
29
23
24
2
51
43
59
32
36
33
17
34
44
24
3
Sec
43.20
35.88
39.12
50.28
52.56
15.60
44.76
18.48
52.80
38.04
38.46
44.88
12.48
48.60
52.80
52.20
7.68
30.84
42.36
20.88
2.52
28.32
18.05
2.76
56.88
37.80
Latitude
Deg
37
37
37
37
37
37
36
37
29
31
30
32
32
30
29
30
30
31
29
30
29
29
29
32
30
32
Min
53
18
16
47
41
14
58
1
40
33
11
10
26
50
56
40
7
40
26
46
56
59
55
20
15
35
Sec
13.56
27.72
55.92
52.08
6.00
0.60
26.40
24.24
5.88
23.76
33.14
23.16
17.16
15.36
30.48
18.84
44.76
3.72
29.76
41.88
22.56
52.80
14.95
58.56
23.40
16.44
10 of 35
-------
OWOW99 Site-ID
OWOW99-1124
OWOW99-1374
OWOW99-1375
OWOW99-1424
OWOW99-1474
OWOW99-1499
OWOW99-1548
OWOW99-1549
OWOW99-0017
OWOW99-0043
OWOW99-0467
OWOW99-0493
OWOW99-0567
OWOW99-0592
OWOW99-0992
OWOW99-1017
OWOW99-1443
OWOW99-0563
OWOW99-0564
OWOW99-1439
OWOW99-0042
OWOW99-0092
OWOW99-0166
OWOW99-0192
OWOW99-0210
OWOW99-0217
Long. DD
-89.3347
-92.3550
-89.6034
-89.8663
-91.7324
-91.5497
-91.9289
-91.2212
-71.1075
-71.0101
-70.0928
-71.9988
-72.3087
-71.8687
-71.6047
-70.6276
-71.7692
-76.3694
-75.8240
-79.3215
-69.8635
-68.2270
-68.0095
-70.5879
-69.3678
-70.8049
Lat. DD
29.8557
30.7519
29.5372
29.6994
30.7120
31.4483
32.8645
32.0874
41.7031
42.6178
41.7239
42.5272
42.4015
42.1353
42.2435
41.3523
42.5272
38.8416
38.0482
39.5043
45.1845
44.8762
44.9731
44.1533
45.8203
44.0539
Lake Name
Christmas Camp Lake?
Miller's Lake
California Bav?
Unknown
Unknown
Old River
Lake Bussy Brake
Lake Bruin
North Watuppa Pond
Prichards Pond
Seymour Pond
Bents Pond
QUABBIN RES
Carbuncle Pond
Westboro Reservoir
Big Homer Pond
Rockwell Pond
Holligans Snooze Inlet
Annemessex River
Deep Creek Lake
Heald Ponds
Lower Middle Branch
Pond
Stiles Lake
Little Pond
Raaaed Lake
Moose Pond
St.
LA
LA
LA
LA
LA
LA
LA
LA
MA
MA
MA
MA
MA
MA
MA
MA
MA
MD
MD
MD
ME
ME
ME
ME
ME
ME
County
ST BERNARD
EVANGELINE
PLAQUEMINES
PLAQUEMINES
POINTE COUPEE
CONCORDIA
MOREHOUSE
TENSAS
BRISTOL
ESSEX
BARNSTABLE
WORCESTER
WORCESTER
WORCESTER
WORCESTER
DUKES
WORCESTER
QUEEN ANNES
SOMERSET
GARRETT
SOMERSET
HANCOCK
HANCOCK
OXFORD
PISCATAQUIS
CUMBERLAND
Lake
Area
(ha)
21
1246
1302
6
16
3
848
7
674
5
69
9
9536
4
1
13
4
10
1710
1449
9
104
17
11
1047
679
Yr.
3
4
4
4
4
4
4
4
1
1
2
2
2
2
3
3
4
2
2
4
1
1
1
1
1
1
Longitude
Deg
89
92
89
89
91
91
91
91
71
71
70
71
72
71
71
70
71
76
75
79
69
68
68
70
69
70
Min
20
21
36
51
43
32
55
13
6
0
5
59
18
52
36
37
46
22
49
19
51
13
0
35
22
48
Sec
4.92
18.00
12.24
58.68
56.64
58.92
44.04
16.32
27.00
36.36
34.08
55.68
31.32
7.32
16.92
39.36
9.12
9.84
26.40
17.40
48.60
37.20
34.20
16.44
4.08
17.64
Latitude
Deg
29
30
29
29
30
31
32
32
41
42
41
42
42
42
42
41
42
38
38
39
45
44
44
44
45
44
Min
51
45
32
41
42
26
51
5
42
37
43
31
24
8
14
21
31
50
2
30
11
52
58
9
49
3
Sec
20.52
6.84
13.92
57.84
43.20
53.88
52.20
14.64
11.16
4.08
26.04
37.92
5.40
7.08
36.60
8.28
37.92
29.76
53.52
15.48
4.20
34.32
23.16
11.88
13.08
14.04
11 of 35
-------
OWOW99 Site-ID
OWOW99-0492
OWOW99-0516
OWOW99-0566
OWOW99-0617
OWOW99-0635
OWOW99-0642
OWOW99-0660
OWOW99-0667
OWOW99-0917
OWOW99-0935
OWOW99-0966
OWOW99-0967
OWOW99-1041
OWOW99-1067
OWOW99-1366
OWOW99-1391
OWOW99-1392
OWOW99-1442
OWOW99-1460
OWOW99-1560
OWOW99-0009
OWOW99-0014
OWOW99-0016
OWOW99-0116
OWOW99-0159
Long. DD
-69.7220
-67.8207
-68.4982
-70.3992
-68.7143
-69.4533
-69.2984
-70.8288
-67.4489
-69.0874
-67.6423
-70.8544
-68.9015
-70.0304
-69.1132
-68.3404
-70.8790
-70.2829
-69.0628
-69.8704
-85.0115
-83.4149
-86.3480
-85.3849
-87.0669
Lat. DD
45.6786
45.1594
44.6483
44.0212
47.1056
44.0098
46.1063
44.8868
44.7863
46.5076
45.6216
43.9330
45.6875
44.4858
44.2628
45.6371
43.6124
45.6192
46.3064
45.9150
45.3005
43.0991
41.9271
42.3976
46.3338
Lake Name
Moosehead Lake
Little River Lake
Green Lake
Middle Range Pond
Wallagrass Lakes
McCurdy Pond
Cuxabexis Lake
Mooselookmeguntic
Lake
Hadley Lake
Peaked Mountain Pond
Spednik Lake
Unnamed
Pemadumcook Lake
Parker Pond
Megunticook Lake
Unnamed
Mud Pond
Wood Pond
Chandler Pond
Seboomook Lake
Walloon Lake
West Lake #1
Lake Chapin
Torch Lake
Dorsev Lake
St.
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
ME
Ml
Ml
Ml
Ml
Ml
County
WASHINGTON
HANCOCK
ANDROSCOGGIN
AROOSTOOK
LINCOLN
PISCATAQUIS
OXFORD
WASHINGTON
PISCATAQUIS
WASHINGTON
OXFORD
PISCATAQUIS
KENNEBEC
WALDO
AROOSTOOK
YORK
SOMERSET
PISCATAQUIS
SOMERSET
EMMET
LAPEER
BERRIEN
KALAMAZOO
ALGER
Lake
Area
(ha)
30308
29
1267
15
100
80
247
6597
680
5
5571
7
7453
611
574
5
8
819
52
2571
1832
1
220
13
16
Yr.
2
2
2
2
2
2
2
2
3
3
3
3
3
3
4
4
4
4
4
4
1
1
1
1
1
Longitude
Deg
69
67
68
70
68
69
69
70
67
69
67
70
68
70
69
68
70
70
69
69
85
83
86
85
87
Min
43
49
29
23
42
27
17
49
26
5
38
51
54
1
6
20
52
16
3
52
0
24
20
23
4
Sec
19.09
14.52
53.52
57.12
51.48
11.88
54.24
43.68
56.04
14.64
32.28
15.84
5.40
49.44
47.52
25.44
44.40
58.44
46.08
13.44
41.40
53.64
52.80
5.64
0.84
Latitude
Deg
45
45
44
44
47
44
46
44
44
46
45
43
45
44
44
45
43
45
46
45
45
43
41
42
46
Min
40
9
38
1
6
0
6
53
47
30
37
55
41
29
15
38
36
37
18
54
18
5
55
23
20
Sec
43.10
33.84
53.88
16.32
20.16
35.28
22.68
12.48
10.68
27.36
17.76
58.80
15.00
8.88
46.08
13.56
44.64
9.12
23.04
54.00
1.80
56.76
37.56
51.36
1.68
12 of 35
-------
OWOW99 Site-ID
OWOW99-0184
OWOW99-0189
OWOW99-0209
OWOW99-0214
OWOW99-0459
OWOW99-0464
OWOW99-0509
OWOW99-0534
OWOW99-0539
OWOW99-0589
OWOW99-0591
OWOW99-0609
OWOW99-0634
OWOW99-0639
OWOW99-0659
OWOW99-0664
OWOW99-0934
OWOW99-0939
OWOW99-0984
OWOW99-0989
OWOW99-1016
OWOW99-1064
OWOW99-1116
OWOW99-1384
OWOW99-1414
OWOW99-1459
Long. DD
-85.5683
-85.3889
-85.9940
-84.7831
-84.6654
-83.5643
-88.0520
-88.8515
-85.2538
-84.2805
-86.1248
-89.1549
-85.3152
-84.7165
-84.7507
-84.2082
-86.0872
-86.1309
-83.5097
-83.5418
-85.5184
-85.3592
-85.5206
-87.9626
-84.2279
-86.0181
Lat. DD
44.5887
44.0069
46.2242
42.5946
45.4599
42.6691
46.5487
46.8884
43.3313
44.4161
42.2524
46.1527
44.9782
44.3499
45.6852
42.1467
46.5590
44.0559
45.0968
42.6689
41.8123
43.1538
42.1948
46.6857
41.7327
44.8708
Lake Name
Hackman Lake
Boot Lake
TPool
Unnamed
Burt Lake
White Lake
Mud Lake
Lake Roland
Spring Lake
Horseshoe Lake
Dyer Lake
Birch Lake
Torch Lake
Houghton Lake
Lake Paradise
Norvell Lake
Cloverleaf Lake
Mud Lake
Seven Mile Pond
Grass Lake
Tamarack Lake
Little Wabasis Lake
Long Lake
Lake Margaret
Glen Lake
St.
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
Ml
County
GRAND
TRAVERSE
OSCEOLA
SCHOOLCRAFT
EATON
CHEBOYGAN
OAKLAND
MARQUETTE
HOUGHTON
MONTCALM
OGEMAW
VAN BUREN
GOGEBIC
ANTRIM
ROSCOMMON
EMMET
JACKSON
ALGER
MASON
ALPENA
OAKLAND
ST JOSEPH
KENT
KALAMAZOO
MARQUETTE
LENAWEE
LEELANAU
Lake
Area
(ha)
7
3
95
6
0
198
2
107
22
14
12
84
7503
8068
767
12
5
2
556
15
52
14
198
52
6
560
Yr.
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
3
3
3
3
3
3
3
4
4
4
Longitude
Deg
85
85
85
84
84
83
88
88
85
84
86
89
85
84
84
84
86
86
83
83
85
85
85
87
84
86
Min
34
23
59
46
39
33
3
51
15
16
7
9
18
42
45
12
5
7
30
32
31
21
31
57
13
1
Sec
5.88
20.04
38.40
59.16
55.58
51.48
7.20
5.40
13.68
49.80
29.28
17.64
54.72
59.40
2.52
29.52
13.92
51.24
34.92
30.48
6.24
33.12
14.16
45.36
40.44
5.16
Latitude
Deg
44
44
46
42
45
42
46
46
43
44
42
46
44
44
45
42
46
44
45
42
41
43
42
46
41
44
Min
35
0
13
35
27
40
32
53
19
24
15
9
58
20
41
8
33
3
5
40
48
9
11
41
43
52
Sec
19.32
24.84
27.12
40.56
35.78
8.76
55.32
18.24
52.68
57.96
8.64
9.72
41.52
59.64
6.72
48.12
32.40
21.24
48.48
8.04
44.28
13.68
41.28
8.52
57.72
14.88
13 of 35
-------
OWOW99 Site-ID
OWOW99-1464
OWOW99-1489
OWOW99-1534
OWOW99-1559
OWOW99-1564
OWOW99-0005
OWOW99-0010
OWOW99-0031
OWOW99-0032
OWOW99-0033
OWOW99-0035
OWOW99-0055
OWOW99-0057
OWOW99-0081
OWOW99-0083
OWOW99-0085
OWOW99-0106
OWOW99-0110
OWOW99-0130
OWOW99-0132
OWOW99-0155
OWOW99-0157
OWOW99-0180
OWOW99-0182
OWOW99-0183
OWOW99-0205
Long. DD
-83.5503
-84.5111
-89.5863
-83.6934
-83.8948
-95.1647
-90.9426
-94.9994
-96.3941
-93.5870
-91.1740
-93.5770
-95.6236
-95.9084
-94.2868
-90.4943
-95.4026
-92.8239
-92.1057
-95.8216
-94.2687
-94.6157
-94.2727
-93.5168
-92.7562
-94.5317
Lat. DD
43.1634
43.1184
46.5082
46.0584
42.5038
47.3415
48.0215
46.8863
45.5205
45.4859
48.0688
47.1810
45.7808
46.7805
46.3518
47.8652
47.1965
48.5579
47.3197
45.6412
46.4872
45.3749
46.9586
44.7412
46.3802
47.4232
Lake Name
Unnamed
Goaebic Lake
Chenango Lake (Lime
Lake)
LaSalle
Mora
Long
Unnamed
Cantlin
Kekekabic
Pokeaama Lake
Unnamed
Fox
White Sand
Dick
Glanders
Namakan Lake
Linwood Lake??
Unnamed
Hubert
Rice
Woman
O'Dowd
Sturaeon
Cass Lake
St.
Ml
Ml
Ml
Ml
Ml
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
County
GENESEE
GRATIOT
CHIPPEWA
LIVINGSTON
HUBBARD
COOK
HUBBARD
BIG STONE
SHERBURNE
LAKE
ITASCA
DOUGLAS
BECKER
CROW WING
COOK
CLEARWATER
ST LOUIS
STEVENS
CROW WING
STEARNS
CASS
SCOTT
PINE
Lake
Area
(ha)
2
1375
5170
29
12
90
94
784
6
41
691
6313
8
56
159
53
20
5686
3
8
511
618
2396
118
666
12050
Yr.
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Longitude
Deg
83
84
89
83
83
95
90
94
96
93
91
93
95
95
94
90
95
92
92
95
94
94
94
93
92
94
Min
33
30
35
41
53
9
56
59
23
35
10
34
37
54
17
29
24
49
6
49
16
36
16
31
45
31
Sec
1.08
39.96
10.50
36.24
41.28
52.92
33.36
57.84
38.76
13.20
26.40
37.20
24.96
30.24
12.48
39.48
9.36
25.93
20.52
17.76
7.32
56.52
21.72
0.48
22.32
53.94
Latitude
Deg
43
43
46
46
42
47
48
46
45
45
48
47
45
46
46
47
47
48
47
45
46
45
46
44
46
47
Min
9
7
30
3
30
20
1
53
31
29
4
10
46
46
21
51
11
33
19
38
29
22
57
44
22
25
Sec
48.24
6.24
29.56
30.24
13.68
29.40
17.40
10.68
13.80
9.24
7.68
51.60
50.88
49.80
6.48
54.72
47.40
28.51
10.92
28.32
13.92
29.64
30.96
28.32
48.72
23.48
14 of 35
-------
OWOW99 Site-ID
OWOW99-0207
OWOW99-0455
OWOW99-0460
OWOW99-0481
OWOW99-0482
OWOW99-0483
OWOW99-0485
OWOW99-0505
OWOW99-0507
OWOW99-0530
OWOW99-0532
OWOW99-0555
OWOW99-0556
OWOW99-0557
OWOW99-0559
OWOW99-0581
OWOW99-0583
OWOW99-0585
OWOW99-0605
OWOW99-0607
OWOW99-0630
OWOW99-0632
OWOW99-0633
OWOW99-0655
OWOW99-0905
OWOW99-0906
Long. DD
-93.2741
-93.5638
-91.5581
-95.5381
-96.0942
-94.0019
-92.0778
-96.0091
-94.1022
-92.9112
-94.5663
-95.8735
-94.4671
-95.3693
-90.6578
-95.7940
-92.8785
-93.1006
-92.2737
-95.3151
-93.9130
-93.1093
-92.8199
-94.8418
-95.9718
-95.4222
Lat. DD
43.7920
47.5288
47.6143
47.0775
45.3626
45.5746
48.2927
46.3972
45.3217
46.8136
45.5608
46.6991
43.6875
46.0890
47.9947
47.4786
45.2609
48.2787
46.9391
45.5396
46.4961
44.8687
46.3146
47.6093
48.3293
46.3986
Lake Name
Geneva
Pickerel
South McDouaal
Many Point
Unnamed
Unnamed
LAC LA CROIX
Wolf
Bass
Moberg
Lauer
Unnamed
Buffalo
Unnamed
Trurno
Unnamed
Unnamed
Unnamed
Fish Lake Reservoir
Swenoda
Agate
Unnamed
First
Fox
Mud Lake
East Leaf
St.
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
County
FREEBORN
ITASCA
LAKE
BECKER
SWIFT
BENTON
ST LOUIS
OTTER TAIL
WRIGHT
ST LOUIS
STEARNS
OTTER TAIL
MARTIN
DOUGLAS
COOK
MAHNOMEN
WASHINGTON
KOOCHICHING
ST LOUIS
POPE
CROW WING
DAKOTA
PINE
BELTRAMI
OTTER TAIL
Lake
Area
(ha)
694
16
113
677
11
2
5769
16
86
14
5
5
103
7
14
8
21
1
1214
117
66
2
31
64
9591
170
Yr.
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
3
3
Longitude
Deg
93
93
91
95
96
94
92
96
94
92
94
95
94
95
90
95
92
93
92
95
93
93
92
94
95
95
Min
16
33
33
32
5
0
4
0
6
54
33
52
28
22
39
47
52
6
16
18
54
6
49
50
58
25
Sec
26.76
49.68
29.16
17.16
39.12
6.84
40.08
32.76
7.92
40.32
58.68
24.60
1.56
9.48
28.08
38.40
42.60
2.16
25.32
54.36
46.80
33.48
11.64
30.48
18.48
19.92
Latitude
Deg
43
47
47
47
45
45
48
46
45
46
45
46
43
46
47
47
45
48
46
45
46
44
46
47
48
46
Min
47
31
36
4
21
34
17
23
19
48
33
41
41
5
59
28
15
16
56
32
29
52
18
36
19
23
Sec
31.20
43.68
51.48
39.00
45.36
28.56
33.72
49.92
18.12
48.96
38.88
56.76
15.00
20.40
40.92
42.96
39.24
43.32
20.76
22.56
45.96
7.32
52.56
33.48
45.55
54.96
15 of 35
-------
OWOW99 Site-ID
OWOW99-0908
OWOW99-0910
OWOW99-0930
OWOW99-0932
OWOW99-0933
OWOW99-0955
OWOW99-0957
OWOW99-0980
OWOW99-0985
OWOW99-1006
OWOW99-1008
OWOW99-1010
OWOW99-1030
OWOW99-1032
OWOW99-1034
OWOW99-1055
OWOW99-1057
OWOW99-1080
OWOW99-1082
OWOW99-1110
OWOW99-1355
OWOW99-1357
OWOW99-1359
OWOW99-1380
OWOW99-1382
OWOW99-1410
Long. DD
-94.6005
-90.9841
-95.5579
-91.6562
-93.6431
-95.9853
-95.0638
-95.0251
-91.2914
-96.1832
-93.0521
-91.7537
-96.2475
-93.6363
-93.0806
-94.3916
-93.8774
-93.4365
-95.6831
-92.3073
-95.8561
-94.4259
-94.9752
-95.7993
-94.8427
-91.9695
Lat. DD
46.2537
48.0774
47.6234
44.0415
46.2381
46.0809
45.2362
47.9620
47.8110
47.6754
45.1318
47.8983
46.8293
44.9095
48.5981
47.1557
44.2542
47.2130
45.8481
47.8681
46.5908
44.9814
49.3608
46.3063
45.1832
48.0107
Lake Name
Shamineau
Howard Lake
Unnamed
Winona
Miles Lacs
Four Mile
Florida
Lower Red Lake
Isabella
Unnamed
Amelia
White Iron
Unnamed
Minnetonka
Leech Lake
Washington
Unnamed
Unnamed
Vermilion Lake
McCollume
Belle
North Turtle
Diamond
Slim
St.
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
County
MORRISON
COOK
POLK
WINONA
MILLE LACS
GRANT
KANDIYOHI
LAKE
POLK
ANOKA
LAKE
CLAY
HENNEPIN
ST LOUIS
LESUEUR
ITASCA
DOUGLAS
OTTER TAIL
MEEKER
LAKE OF THE
WOODS
OTTER TAIL
KANDIYOHI
ST LOUIS
Lake
Area
(ha)
548
69
13
32
51700
79
211
0
667
7
50
2404
12
1700
1
44280
582
7
14
19875
6
362
114
601
626
131
Yr.
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
4
4
4
4
4
4
Longitude
Deg
94
90
95
91
93
95
95
95
91
96
93
91
96
93
93
94
93
93
95
92
95
94
94
95
94
91
Min
36
59
33
39
38
59
3
1
17
10
3
45
14
38
4
23
52
26
40
18
51
25
58
47
50
58
Sec
1.80
2.76
28.44
22.32
35.16
7.08
49.68
30.29
29.04
59.52
7.56
13.32
51.00
10.68
50.16
29.69
38.64
11.40
59.16
26.17
21.96
33.24
30.72
57.48
33.72
10.20
Latitude
Deg
46
48
47
44
46
46
45
47
47
47
45
47
46
44
48
47
44
47
45
47
46
44
49
46
45
48
Min
15
4
37
2
14
4
14
57
48
40
7
53
49
54
35
9
15
12
50
52
35
58
21
18
10
0
Sec
13.32
38.64
24.24
29.40
17.16
51.24
10.32
43.02
39.60
31.44
54.48
53.88
45.48
34.20
53.16
20.48
15.12
46.80
53.16
5.20
26.88
53.04
38.88
22.68
59.52
38.52
16 of 35
-------
OWOW99 Site-ID
OWOW99-1430
OWOW99-1431
OWOW99-1435
OWOW99-1455
OWOW99-1457
OWOW99-1458
OWOW99-1480
OWOW99-1482
OWOW99-1506
OWOW99-1508
OWOW99-1510
OWOW99-1530
OWOW99-1532
OWOW99-1555
OWOW99-0040
OWOW99-0215
OWOW99-0490
OWOW99-0518
OWOW99-0543
OWOW99-0618
OWOW99-0665
OWOW99-0912
OWOW99-0915
OWOW99-0968
OWOW99-1015
OWOW99-1043
Long. DD
-95.2037
-95.7495
-91.0591
-93.7461
-92.3071
-92.5169
-94.7573
-93.7620
-95.6548
-93.7467
-91.4933
-93.5769
-95.3562
-95.1158
-91.0894
-89.6157
-92.1693
-92.9303
-93.3961
-93.6900
-91.3675
-89.1714
-91.6021
-91.5418
-90.4555
-94.3974
Lat. DD
48.9700
46.4793
48.1618
46.6502
44.5155
46.2546
47.1668
44.4942
46.9790
45.1509
47.9748
47.4910
45.9641
47.7441
38.8129
37.2404
38.9230
38.5295
36.5590
37.3760
38.0854
36.7866
39.2285
37.7095
38.7693
38.7750
Lake Name
Lake of the Woods
Dead
Unnamed
Blind
Lake Pepin
Unnamed
Kabekona
Thomas
Flat
Charlotte
Ennis
Spider
Carlos
Unnamed
TABLE ROCK L
St.
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MN
MO
MO
MO
MO
MO
MO
MO
MO
MO
MO
MO
MO
County
OTTER TAIL
LAKE
AITKIN
GOODHUE
PINE
HUBBARD
LESUEUR
BECKER
WRIGHT
LAKE
ITASCA
DOUGLAS
BELTRAMI
WARREN
CAPE GIRARDEAU
BOONE
MORGAN
STONE
DADE
CRAWFORD
MISSISSIPPI
AUDRAIN
DENT
ST LOUIS
CASS
Lake
Area
(ha)
#####
2988
2
120
5075
10
975
48
741
94
9
546
1040
6
10
128
9
4
12410
3
2
8
8
2
2
12
Yr.
4
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
2
2
2
2
2
3
3
3
3
3
Longitude
Deg
95
95
91
93
92
92
94
93
95
93
91
93
95
95
91
89
92
92
93
93
91
89
91
91
90
94
Min
12
44
3
44
18
31
45
45
39
44
29
34
21
6
5
36
10
55
23
41
22
10
36
32
27
23
Sec
13.25
58.20
32.76
45.96
25.56
0.84
26.28
43.20
17.28
48.12
35.88
36.84
22.32
56.88
21.84
56.52
9.48
49.08
45.96
24.00
3.00
17.04
7.56
30.48
19.80
50.64
Latitude
Deg
48
46
48
46
44
46
47
44
46
45
47
47
45
47
38
37
38
38
36
37
38
36
39
37
38
38
Min
58
28
9
39
30
15
10
29
58
9
58
29
57
44
48
14
55
31
33
22
5
47
13
42
46
46
Sec
12.07
45.48
42.48
0.72
55.80
16.56
0.48
39.12
44.40
3.24
29.28
27.60
50.76
38.76
46.44
25.44
22.80
46.20
32.40
33.60
7.44
11.76
42.60
34.20
9.48
30.00
17 of 35
-------
OWOW99 Site-ID
OWOW99-1068
OWOW99-1393
OWOW99-1437
OWOW99-1440
OWOW99-1490
OWOW99-1515
OWOW99-0071
OWOW99-0098
OWOW99-0146
OWOW99-0172
OWOW99-0472
OWOW99-0521
OWOW99-0522
OWOW99-0546
OWOW99-0572
OWOW99-0621
OWOW99-0624
OWOW99-0672
OWOW99-0949
OWOW99-0997
OWOW99-1047
OWOW99-1096
OWOW99-1122
OWOW99-1372
OWOW99-1397
OWOW99-1422
Long. DD
-94.5328
-93.5719
-89.4497
-91.7100
-92.0686
-91.9827
-91.1601
-88.5441
-89.8375
-90.8814
-88.1104
-90.4070
-90.1623
-90.9335
-89.5842
-90.2024
-90.7826
-89.7129
-89.0588
-89.8621
-88.9294
-89.7340
-88.7112
-90.3281
-90.1054
-89.7740
Lat. DD
37.2897
38.1700
36.5535
39.5129
40.0317
38.9651
33.5082
32.5750
33.4770
32.7220
34.8551
34.0076
32.2687
33.2578
31.6186
33.5276
32.0304
34.4486
30.8977
34.1474
32.8226
33.8319
33.7215
32.2211
32.5856
34.0039
Lake Name
TRUMAN RES
Mark Twain Lake
Sardis Lake
Enid Lake
Grenada Lake
St.
MO
MO
MO
MO
MO
MO
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
County
JASPER
ST CLAIR
NEW MADRID
RALLS
KNOX
CALLAWAY
WASHINGTON
LAUDERDALE
CARROLL
SHARKEY
TISHOMINGO
TALLAHATCHIE
RANKIN
WASHINGTON
COVINGTON
LEFLORE
CLAIBORNE
STONE
NESHOBA
MONROE
HINDS
MADISON
YALOBUSHA
Lake
Area
(ha)
14
9246
10
3551
4
9
47
12
50
10
636
75
4
6
3
1016
37
23684
1
11230
4
26154
5
2
19
6
Yr.
3
4
4
4
4
4
1
1
1
1
2
2
2
2
2
2
2
2
3
3
3
3
3
4
4
4
Longitude
Deg
94
93
89
91
92
91
91
88
89
90
88
90
90
90
89
90
90
89
89
89
88
89
88
90
90
89
Min
31
34
26
42
4
58
9
32
50
52
6
24
9
56
35
12
46
42
3
51
55
44
42
19
6
46
Sec
58.08
18.84
58.92
36.00
6.96
57.72
36.36
38.76
15.00
53.04
37.44
25.20
44.28
0.60
3.12
8.64
57.36
46.48
31.68
43.45
45.84
2.36
40.32
41.16
19.44
26.40
Latitude
Deg
37
38
36
39
40
38
33
32
33
32
34
34
32
33
31
33
32
34
30
34
32
33
33
32
32
34
Min
17
10
33
30
1
57
30
34
28
43
51
0
16
15
37
31
1
26
53
8
49
49
43
13
35
0
Sec
22.92
12.00
12.60
46.44
54.12
54.36
29.52
30.00
37.20
19.20
18.36
27.36
7.32
28.08
6.96
39.36
49.44
55.03
51.72
50.68
21.36
54.80
17.40
15.96
8.16
14.04
18 of 35
-------
OWOW99 Site-ID
OWOW99-1448
OWOW99-1546
OWOW99-1572
OWOW99-1574
OWOW99-0029
OWOW99-0053
OWOW99-0054
OWOW99-0084
OWOW99-0104
OWOW99-0129
OWOW99-0153
OWOW99-0178
OWOW99-0181
OWOW99-0454
OWOW99-0479
OWOW99-0558
OWOW99-0579
OWOW99-0604
OWOW99-0909
OWOW99-0952
OWOW99-1004
OWOW99-1029
OWOW99-1079
OWOW99-1104
OWOW99-1358
OWOW99-1404
Long. DD
-89.3108
-90.0304
-90.0714
-89.5315
-111.2044
-108.1039
-113.7489
-106.7435
-107.4776
-113.3498
-113.1950
-104.6747
-104.5234
-113.9012
-111.5479
-106.4908
-111.5445
-115.2348
-108.5948
-111.2497
-111.9909
-115.6653
-113.8897
-115.4069
-106.0834
-113.2146
Lat. DD
31.3542
33.5995
34.7557
30.3291
48.3775
45.1706
48.6154
47.7335
47.1298
48.8880
44.9973
45.6234
47.2791
47.5570
47.4160
47.6953
46.3699
48.5864
48.3476
44.7870
48.0219
48.2210
48.1628
47.2692
47.8205
47.0855
Lake Name
BIGHORN L
FORT PECK RES
L KOOCANUSA
Hebgen Lake
St.
MS
MS
MS
MS
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
MT
County
FORREST
CARROLL
DE SOTO
HANCOCK
LIBERTY
BIGHORN
GLACIER
VALLEY
GARFIELD
GLACIER
BEAVERHEAD
CARTER
DAWSON
MISSOULA
CASCADE
GARFIELD
BROADWATER
LINCOLN
ELAINE
TETON
LINCOLN
FLATHEAD
MINERAL
MCCONE
POWELL
Lake
Area
(ha)
30
8
3249
2
1076
6943
1
98766
6
6
10
8
2055
23
20
2
196
11463
59
0
9
52
9
3
5
2
Yr.
4
4
4
4
1
1
1
1
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
3
4
4
Longitude
Deg
89
90
90
89
111
108
113
106
107
113
113
104
104
113
111
106
111
115
108
111
111
115
113
115
106
113
Min
18
1
4
31
12
6
44
44
28
20
11
40
31
54
32
29
32
14
35
14
59
39
53
24
5
12
Sec
38.88
49.44
17.04
53.40
15.84
14.04
56.04
36.60
39.36
59.28
42.00
28.92
24.24
4.32
52.44
26.88
40.20
5.28
41.28
58.74
27.24
55.08
22.92
24.84
0.24
52.56
Latitude
Deg
31
33
34
30
48
45
48
47
47
48
44
45
47
47
47
47
46
48
48
44
48
48
48
47
47
47
Min
21
35
45
19
22
10
36
44
7
53
59
37
16
33
24
41
22
35
20
47
1
13
9
16
49
5
Sec
15.12
58.20
20.52
44.76
39.00
14.16
55.44
0.60
47.28
16.80
50.28
24.24
44.76
25.20
57.60
43.08
11.64
11.04
51.36
13.02
18.84
15.60
46.08
9.12
13.80
7.80
19 of 35
-------
OWOW99 Site-ID
OWOW99-1429
OWOW99-1434
OWOW99-1504
OWOW99-1529
OWOW99-0062
OWOW99-0137
OWOW99-0139
OWOW99-0162
OWOW99-0164
OWOW99-0537
OWOW99-0611
OWOW99-0612
OWOW99-0962
OWOW99-1037
OWOW99-1112
OWOW99-1387
OWOW99-1389
OWOW99-0006
OWOW99-0030
OWOW99-0034
OWOW99-0105
OWOW99-0109
OWOW99-0131
OWOW99-0156
OWOW99-0456
Long. DD
-112.4335
-107.2259
-111.6821
-114.0767
-81.4559
-80.4275
-76.4601
-79.0165
-78.0190
-80.9698
-83.3703
-78.9274
-79.8803
-78.4574
-77.2761
-78.6858
-77.2762
-100.0630
-98.8053
-103.9666
-98.9742
-100.2730
-100.9038
-99.9637
-99.0408
Lat. DD
47.9460
48.7055
45.4309
48.8717
35.3010
35.7108
35.7687
35.7732
36.5410
35.3508
35.4456
35.1360
35.7551
35.2586
34.7116
36.0778
36.1907
46.7390
48.2210
48.6934
48.2524
48.8584
46.9226
47.4209
47.1242
Lake Name
Kings Mt. Reservoir
Impoundment/ Grants
Creek
Phelps Lake
B Everett Jordan Lake
Lake Gaston
Mt. Island Lake
Impoundment/Oconoluft
ee River
Smith Lake
Unnamed
Unnamed
Unnamed
Unnamed
Unnamed
Long Lake
Devils Lake
St.
MT
MT
MT
MT
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
NC
ND
ND
ND
ND
ND
ND
ND
ND
County
TETON
PHILLIPS
MADISON
FLATHEAD
CLEVELAND
ROWAN
CHATHAM
WARREN
MECKLENBURG
SWAIN
CUMBERLAND
RANDOLPH
SAMPSON
ONSLOW
GRANVILLE
BERTIE
KIDDER
RAMSEY
DIVIDE
RAMSEY
BOTTINEAU
BURLEIGH
WELLS
STUTSMAN
Lake
Area
(ha)
1296
231
1491
1
552
6
6718
5787
7951
1404
7
34
1
24
6
1
6
1300
7120
9
2196
5
20
37
3
Yr.
4
4
4
4
1
1
1
1
1
2
2
2
3
3
3
4
4
1
1
1
1
1
1
1
2
Longitude
Deg
112
107
111
114
81
80
76
79
78
80
83
78
79
78
77
78
77
100
98
103
98
100
100
99
99
Min
26
13
40
4
27
25
27
0
1
58
22
55
52
27
16
41
16
3
48
57
58
16
54
57
2
Sec
0.60
33.24
55.56
36.12
21.24
39.00
36.18
59.40
8.40
11.28
13.08
38.64
49.08
26.64
33.96
8.88
34.32
46.80
19.08
59.76
27.12
22.80
13.68
49.32
26.88
Latitude
Deg
47
48
45
48
35
35
35
35
36
35
35
35
35
35
34
36
36
46
48
48
48
48
46
47
47
Min
56
42
25
52
18
42
46
46
32
21
26
8
45
15
42
4
11
44
13
41
15
51
55
25
7
Sec
45.60
19.80
51.24
18.12
3.60
38.88
7.36
23.52
27.60
2.88
44.16
9.60
18.36
30.96
41.76
40.08
26.52
20.40
15.60
36.24
8.64
30.24
21.36
15.24
27.12
20 of 35
-------
OWOW99 Site-ID
OWOW99-0480
OWOW99-0484
OWOW99-0506
OWOW99-0580
OWOW99-0584
OWOW99-0606
OWOW99-0656
OWOW99-0956
OWOW99-0981
OWOW99-1005
OWOW99-1009
OWOW99-1081
OWOW99-1105
OWOW99-1106
OWOW99-1109
OWOW99-1381
OWOW99-1405
OWOW99-1406
OWOW99-1409
OWOW99-1456
OWOW99-1505
OWOW99-1509
OWOW99-1531
OWOW99-0003
OWOW99-0065
OWOW99-0094
Long. DD
-98.1807
-103.4168
-99.1740
-100.2759
-102.3091
-100.3287
-98.9907
-99.7841
-99.2248
-99.8568
-102.0806
-101.4909
-97.8659
-97.8346
-100.2276
-99.5410
-99.0504
-99.1631
-99.9233
-99.4723
-100.1783
-101.9556
-102.4403
-99.1880
-96.3294
-96.7822
Lat. DD
47.8124
48.2622
47.1297
47.8418
48.5432
47.7739
46.0214
47.0430
46.6055
48.5667
48.3434
47.9278
47.5268
46.7275
48.9943
47.2677
47.6458
46.9102
48.8529
46.1183
48.1937
48.2697
47.9289
42.2988
42.2335
41.2891
Lake Name
St.
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
NE
NE
NE
County
NELSON
WILLIAMS
STUTSMAN
SHERIDAN
MOUNTRAIL
SHERIDAN
DICKEY
KIDDER
LOGAN
ROLETTE
MOUNTRAIL
WARD
STEELE
BARNES
BOTTINEAU
KIDDER
EDDY
STUTSMAN
ROLETTE
MCINTOSH
PIERCE
MOUNTRAIL
MOUNTRAIL
HOLT
THURSTON
SAUNDERS
Lake
Area
(ha)
13
60
11
24
6
8
12
1356
4
49
4
52
5
13
20
64
16
12
5
204
10
55
2
37
6
3
Yr.
2
2
2
2
2
2
2
3
3
3
3
3
3
3
3
4
4
4
4
4
4
4
4
1
1
1
Longitude
Deg
98
103
99
100
102
100
98
99
99
99
102
101
97
97
100
99
99
99
99
99
100
101
102
99
96
96
Min
10
25
10
16
18
19
59
47
13
51
4
29
51
50
13
32
3
9
55
28
10
57
26
11
19
46
Sec
50.52
0.48
26.40
33.24
32.76
43.32
26.52
2.76
29.28
24.48
50.16
27.24
57.24
4.56
39.36
27.60
1.44
47.16
23.88
20.28
41.88
20.16
25.08
16.80
45.84
55.92
Latitude
Deg
47
48
47
47
48
47
46
47
46
48
48
47
47
46
48
47
47
46
48
46
48
48
47
42
42
41
Min
48
15
7
50
32
46
1
2
36
34
20
55
31
43
59
16
38
54
51
7
11
16
55
17
14
17
Sec
44.64
43.92
46.92
30.48
35.52
26.04
17.04
34.80
19.80
0.12
36.24
40.08
36.48
39.00
39.48
3.72
44.88
36.72
10.44
5.88
37.32
10.92
44.04
55.68
0.60
20.76
21 of 35
-------
OWOW99 Site-ID
OWOW99-0103
OWOW99-0453
OWOW99-0494
OWOW99-0578
OWOW99-0594
OWOW99-0919
OWOW99-0978
OWOW99-1103
OWOW99-1356
OWOW99-1403
OWOW99-1419
OWOW99-1444
OWOW99-1540
OWOW99-0142
OWOW99-0167
OWOW99-0517
OWOW99-0918
OWOW99-1367
OWOW99-0013
OWOW99-0463
OWOW99-0638
OWOW99-1063
OWOW99-1413
OWOW99-1563
OWOW99-0095
OWOW99-0169
Long. DD
-102.7355
-103.4950
-100.4095
-102.3409
-98.1846
-96.9633
-101.7811
-102.5531
-98.1129
-101.8481
-95.6776
-101.5539
-96.3740
-71.1640
-71.3410
-71.7674
-71.7401
-71.9652
-74.8627
-74.2645
-74.2684
-74.2473
-75.2780
-74.9980
-103.6595
-107.6105
Lat. DD
42.0064
41.9337
40.9410
42.0069
40.0087
41.4518
42.4001
42.2118
42.7988
41.2503
40.3263
40.4322
41.3094
43.0991
43.6026
43.6595
43.0228
43.3070
39.7848
39.7364
41.1211
40.8269
39.3922
40.9226
35.0939
36.5178
Lake Name
L MCCONAUGHY
Unnamed
Lake Winneoesaukee
Newfound Lake
Unnamed
Loch Lyndon Reservoir
Arch Hurly Conservency
Lake
Mavajo Reservoir
St.
NE
NE
NE
NE
NE
NE
NE
NE
NE
NE
NE
NE
NE
NH
NH
NH
NH
NH
NJ
NJ
NJ
NJ
NJ
NJ
NM
NM
County
BOX BUTTE
SCOTTS BLUFF
LINCOLN
GARDEN
NUCKOLLS
COLFAX
CHERRY
SHERIDAN
KNOX
KEITH
NEMAHA
CHASE
DOUGLAS
ROCKINGHAM
BELKNAP
GRAFTON
HILLSBOROUGH
MERRIMACK
CAMDEN
OCEAN
PASSAIC
ESSEX
CUMBERLAND
WARREN
QUAY
RIOARRIBA
Lake
Area
(ha)
8
784
226
10
7
5
10
7
28
11464
2
652
9
5
18545
1718
8
47
4
18
23
5
3
6
39
1892
Yr.
1
2
2
2
2
3
3
3
4
4
4
4
4
1
1
2
3
4
1
2
2
3
4
4
1
1
Longitude
Deg
102
103
100
102
98
96
101
102
98
101
95
101
96
71
71
71
71
71
74
74
74
74
75
74
103
107
Min
44
29
24
20
11
57
46
33
6
50
40
33
22
9
20
46
44
57
51
15
16
14
16
59
39
36
Sec
7.80
42.00
34.20
27.24
4.56
47.88
51.96
11.16
46.44
53.16
39.36
14.04
26.40
50.40
27.60
2.64
24.36
54.72
45.72
52.20
6.24
50.28
40.80
52.80
34.20
37.80
Latitude
Deg
42
41
40
42
40
41
42
42
42
41
40
40
41
43
43
43
43
43
39
39
41
40
39
40
35
36
Min
0
56
56
0
0
27
24
12
47
15
19
25
18
5
36
39
1
18
47
44
7
49
23
55
5
31
Sec
23.04
1.32
27.60
24.84
31.32
6.48
0.36
42.48
55.68
1.08
34.68
55.92
33.84
56.76
9.36
34.20
22.08
25.20
5.28
11.04
15.96
36.84
31.92
21.36
38.04
4.08
22 of 35
-------
OWOW99 Site-ID
OWOW99-0519
OWOW99-1369
OWOW99-1445
OWOW99-0501
OWOW99-0652
OWOW99-0902
OWOW99-0926
OWOW99-0951
OWOW99-1102
OWOW99-1376
OWOW99-1451
OWOW99-1552
OWOW99-0038
OWOW99-0063
OWOW99-0067
OWOW99-0088
OWOW99-0113
OWOW99-0114
OWOW99-0138
OWOW99-0488
OWOW99-0538
OWOW99-0542
OWOW99-0562
OWOW99-0588
OWOW99-0593
OWOW99-0613
Long. DD
-105.0750
-104.3510
-103.9262
-118.3701
-114.3731
-119.5533
-115.4695
-118.3443
-116.0292
-118.3889
-117.1533
-116.2257
-75.5268
-74.7667
-74.5002
-76.9186
-75.4139
-79.3778
-73.5965
-74.1190
-74.5199
-74.4799
-78.8773
-75.2920
-73.7070
-73.7041
Lat. DD
36.8757
32.6128
33.0108
38.5316
36.2826
40.0220
40.1724
38.9805
38.9549
38.8915
41.4146
36.3573
42.5710
41.7675
44.1914
42.6277
44.2527
42.1331
42.1441
42.2356
41.6999
44.2537
42.6172
44.1313
43.7154
41.5026
Lake Name
No name playa lake
Brantley Reservoir
No name plava lake
LAKE MEAD
Pyramid Lake
Ruby Lake
Chimney Reservoir
Mead Pond
Lake Barnabee
Tuooer Lake
Seneca Lake
Sylvia Lake
Chatauqua Lake
Copake Lake
Colgate Lake
Unnamed
Little Wolf Pond
Unnamed
Unnamed
Brant Lake
Southern South Lake
St.
NM
NM
NM
NV
NV
NV
NV
NV
NV
NV
NV
NV
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
County
COLFAX
CHAVES
MINERAL
CLARK
WASHOE
ELKO
MINERAL
NYE
MINERAL
HUMBOLDT
NYE
CHENANGO
SULLIVAN
FRANKLIN
YATES
ST LAWRENCE
COLUMBIA
GREENE
SULLIVAN
FRANKLIN
ERIE
LEWIS
WARREN
PUTNAM
Lake
Area
(ha)
6
8498
1
82
39373
44233
38
84
269
277
881
138
5
2
2584
17413
125
5438
158
11
7
65
2
1
572
4
Yr.
2
4
4
2
2
3
3
3
3
4
4
4
1
1
1
1
1
1
1
2
2
2
2
2
2
2
Longitude
Deg
105
104
103
118
114
119
115
118
116
118
117
116
75
74
74
76
75
79
73
74
74
74
78
75
73
73
Min
4
21
55
22
22
33
28
20
1
23
9
13
31
46
30
55
24
22
35
7
31
28
52
17
42
42
Sec
30.00
3.46
34.32
12.36
23.16
11.88
10.20
39.48
45.12
20.04
11.88
32.52
36.48
0.12
0.72
6.96
50.04
40.12
47.40
8.40
11.64
47.64
38.28
31.20
25.20
14.76
Latitude
Deg
36
32
33
38
36
40
40
38
38
38
41
36
42
41
44
42
44
42
42
42
41
44
42
44
43
41
Min
52
36
0
31
16
1
10
58
57
53
24
21
34
46
11
37
15
7
8
14
41
15
37
7
42
30
Sec
32.52
46.19
38.88
53.76
57.36
19.20
20.64
49.80
17.64
29.40
52.56
26.28
15.60
3.00
29.04
39.72
9.72
59.20
38.76
8.16
59.64
13.32
1.92
52.68
55.44
9.36
23 of 35
-------
OWOW99 Site-ID
OWOW99-0913
OWOW99-1013
OWOW99-1018
OWOW99-1362
OWOW99-1438
OWOW99-1463
OWOW99-1488
OWOW99-1492
OWOW99-1513
OWOW99-1518
OWOW99-1542
OWOW99-0066
OWOW99-0163
OWOW99-0513
OWOW99-0541
OWOW99-0963
OWOW99-1038
OWOW99-1066
OWOW99-1091
OWOW99-1114
OWOW99-1363
OWOW99-1388
OWOW99-1466
OWOW99-1491
OWOW99-1514
OWOW99-0023
Long. DD
-75.9898
-74.1704
-73.6822
-77.1845
-76.2675
-72.3292
-73.9587
-74.5445
-74.8449
-73.5952
-73.9226
-82.5221
-81.7622
-82.2338
-83.1276
-83.5746
-81.6191
-84.0402
-84.1174
-83.6802
-81.2526
-81.1403
-84.7258
-82.2990
-80.5713
-96.3983
Lat. DD
43.4336
43.2291
43.3046
43.2367
43.2846
40.9420
41.1617
44.3974
43.8336
43.8205
44.5617
39.1898
40.1524
39.9526
40.6223
41.0512
41.0094
39.8466
39.5665
41.6072
41.3496
40.7884
39.2000
39.7671
41.3069
33.9319
Lake Name
Whitney Pond
Northville Lake
Unnamed
Unnamed
Pennellville Pond
Goldfish Pond
DeForest Lake
Little Rock Pond
Moose Lake
Grizzle Ocean
Mud Pond
Lake Rupert
Unnamed
Unnamed
Unnamed
Branch Lake#1
Unnamed
Unnamed
Unnamed
Unnamed Lake
Hidden Valley Lake
Unnamed Lake
Clouse Lake
Unnamed Lake
St.
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
NY
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OH
OK
County
OSWEGO
FULTON
WARREN
WAYNE
OSWEGO
SUFFOLK
ROCKLAND
FRANKLIN
HERKIMER
ESSEX
CLINTON
VINTON
MUSKINGUM
LICKING
MARION
HANCOCK
SUMMIT
GREENE
WARREN
LUCAS
GEAUGA
STARK
HAMILTON
PERRY
TRUMBULL
BRYAN
Lake
Area
(ha)
32
8
7
1
7
1
94
7
507
8
45
133
1542
2
2
1
3
9
4
5
2
2
5
13
2
5
Yr.
3
3
3
4
4
4
4
4
4
4
4
1
1
2
2
3
3
3
3
3
4
4
4
4
4
1
Longitude
Deg
75
74
73
77
76
72
73
74
74
73
73
82
81
82
83
83
81
84
84
83
81
81
84
82
80
96
Min
59
10
40
11
16
19
57
32
50
35
55
31
45
14
7
34
37
2
7
40
15
8
43
17
34
23
Sec
23.28
13.44
55.92
4.20
3.00
45.12
31.32
40.20
41.64
42.72
21.36
19.56
43.92
1.68
39.36
28.56
8.76
24.72
2.64
48.72
9.36
25.08
32.88
56.40
16.68
53.88
Latitude
Deg
43
43
43
43
43
40
41
44
43
43
44
39
40
39
40
41
41
39
39
41
41
40
39
39
41
33
Min
26
13
18
14
17
56
9
23
50
49
33
11
9
57
37
3
0
50
33
36
20
47
12
46
18
55
Sec
0.96
44.76
16.56
12.12
4.56
31.20
42.12
50.64
0.96
13.80
42.12
23.28
8.64
9.36
20.28
4.32
33.84
47.76
59.40
25.92
58.56
18.24
0.00
1.56
24.84
54.84
24 of 35
-------
OWOW99 Site-ID
OWOW99-0049
OWOW99-0068
OWOW99-0069
OWOW99-0099
OWOW99-0193
OWOW99-0194
OWOW99-0219
OWOW99-0499
OWOW99-0544
OWOW99-0643
OWOW99-0644
OWOW99-0669
OWOW99-0924
OWOW99-0944
OWOW99-1023
OWOW99-1024
OWOW99-1069
OWOW99-1093
OWOW99-1123
OWOW99-1423
OWOW99-1468
OWOW99-1469
OWOW99-1494
OWOW99-1524
OWOW99-1543
OWOW99-1544
Long. DD
-94.8975
-95.5922
-98.4909
-95.4239
-95.3515
-95.8195
-96.3680
-94.6797
-97.5291
-95.3119
-95.2973
-96.7935
-96.2380
-97.9921
-98.0545
-95.1480
-98.6438
-95.1917
-96.5470
-97.6357
-94.9559
-96.5172
-99.3117
-94.8057
-95.6455
-98.1776
Lat. DD
34.9442
36.5821
35.1982
34.0857
35.9778
35.3984
36.2481
34.2803
34.9868
36.0564
35.4847
36.6135
34.5780
35.5221
34.3044
35.2651
35.4976
36.4339
34.4187
34.5867
35.7116
35.1970
34.9257
35.2691
36.5462
36.7337
Lake Name
OOLOGAH L
Hugo Lake
KEYSTONE L
BROKEN BOW L
TENKILLER FERRY L
St.
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
County
LE FLORE
ROGERS
CADDO
CHOCTAW
WAGONER
MCINTOSH
PAWNEE
MCCURTAIN
MCCLAIN
WAGONER
MUSKOGEE
OSAGE
COAL
CANADIAN
STEPHENS
HASKELL
CUSTER
MAYES
JOHNSTON
STEPHENS
CHEROKEE
SEMINOLE
KIOWA
LE FLORE
ROGERS
ALFALFA
Lake
Area
(ha)
1
6100
1654
4950
634
590
5455
5342
12
799
1
2
159
63
2076
6
4
8
41
15
5350
145
1810
1
99
4041
Yr.
1
1
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
3
3
4
4
4
4
4
4
4
Longitude
Deg
94
95
98
95
95
95
96
94
97
95
95
96
96
97
98
95
98
95
96
97
94
96
99
94
95
98
Min
53
35
29
25
21
49
22
40
31
18
17
47
14
59
3
8
38
11
32
38
57
31
18
48
38
10
Sec
51.00
31.92
27.24
26.04
5.40
10.20
4.80
46.92
44.76
42.84
50.28
36.60
16.80
31.56
16.20
52.80
37.68
30.12
49.20
8.52
21.24
1.92
42.12
20.52
43.80
39.36
Latitude
Deg
34
36
35
34
35
35
36
34
34
36
35
36
34
35
34
35
35
36
34
34
35
35
34
35
36
36
Min
56
34
11
5
58
23
14
16
59
3
29
36
34
31
18
15
29
26
25
35
42
11
55
16
32
44
Sec
39.12
55.56
53.52
8.52
40.08
54.24
53.16
49.08
12.48
23.04
4.92
48.60
40.80
19.56
15.84
54.36
51.36
2.04
7.32
12.12
41.76
49.20
32.52
8.76
46.32
1.32
25 of 35
-------
OWOW99 Site-ID
OWOW99-0076
OWOW99-0101
OWOW99-0451
OWOW99-0576
OWOW99-0629
OWOW99-0901
OWOW99-0929
OWOW99-0976
OWOW99-1001
OWOW99-1101
OWOW99-1353
OWOW99-1401
OWOW99-1454
OWOW99-1501
OWOW99-0039
OWOW99-0089
OWOW99-0188
OWOW99-0213
OWOW99-0489
OWOW99-0663
OWOW99-0988
OWOW99-1014
OWOW99-1088
OWOW99-1113
OWOW99-1417
OWOW99-1517
Long. DD
-123.2389
-119.2216
-122.0948
-119.1225
-120.5315
-122.1189
-123.8054
-120.0245
-123.2441
-120.2574
-117.3510
-122.0095
-123.3889
-121.7221
-78.6659
-77.8121
-74.9514
-76.3888
-79.4857
-76.2884
-75.6005
-80.4247
-75.2502
-76.7346
-71.5789
-71.4594
Lat. DD
44.5527
43.4362
42.9494
43.2360
45.7258
44.8230
46.1084
42.1399
43.3729
42.6017
43.4992
42.2938
45.4452
43.6916
41.1581
39.9451
41.2504
41.9443
40.6822
41.2899
40.5611
41.2928
41.4692
40.1593
41.1687
41.7052
Lake Name
Crater Lake
Harney Lake
Lake Umatilla
Lake Abert
Lake Sabula
unnamed pond
Pike Lake #3
unnamed pond
Crooked Creek Lake
Luzerne Lake #6
Lehigh Lake #7
Shenango River
Reservoir
Wayne-Whitney Lake
York Haven Dam
Fresh Pond
Gorton Pond?
St.
OR
OR
OR
OR
OR
OR
OR
OR
OR
OR
OR
OR
OR
OR
PA
PA
PA
PA
PA
PA
PA
PA
PA
PA
Rl
Rl
County
LINN
HARNEY
KLAMATH
KLICKITAT
MARION
CLATSOP
LAKE
DOUGLAS
LAKE
MALHEUR
KLAMATH
WASHINGTON
DESCHUTES
CLEARFIELD
FRANKLIN
PIKE
BRADFORD
ARMSTRONG
LUZERNE
LEHIGH
MERCER
WAYNE
DAUPHIN
WASHINGTON
KENT
Lake
Area
(ha)
7
57
5318
9844
11698
26
10
767
6
16397
4577
1498
81
4110
13
2
6
10
151
18
2
1491
46
1596
2526
22
Yr.
1
1
2
2
2
3
3
3
3
3
4
4
4
4
1
1
1
1
2
2
3
3
3
3
4
4
Longitude
Deg
123
119
122
119
120
122
123
120
123
120
117
122
123
121
78
77
74
76
79
76
75
80
75
76
71
71
Min
14
13
5
7
31
7
48
1
14
15
21
0
23
43
39
48
57
23
29
17
36
25
15
44
34
27
Sec
20.11
17.87
41.10
21.14
53.54
7.97
19.33
28.20
38.72
26.60
3.67
34.13
19.97
19.67
57.24
43.56
5.04
19.68
8.52
18.24
1.80
28.92
0.72
4.56
44.04
33.84
Latitude
Deg
44
43
42
43
45
44
46
42
43
42
43
42
45
43
41
39
41
41
40
41
40
41
41
40
41
41
Min
33
26
56
14
43
49
6
8
22
36
29
17
26
41
9
56
15
56
40
17
33
17
28
9
10
42
Sec
9.54
10.28
57.84
9.67
32.92
22.87
30.06
23.64
22.48
6.08
57.08
37.82
42.61
29.87
29.16
42.36
1.44
39.48
55.92
23.64
39.96
34.08
9.12
33.48
7.32
18.72
26 of 35
-------
OWOW99 Site-ID
OWOW99-1567
OWOW99-0012
OWOW99-0112
OWOW99-0211
OWOW99-0212
OWOW99-0462
OWOW99-0536
OWOW99-0662
OWOW99-0936
OWOW99-0937
OWOW99-0987
OWOW99-1061
OWOW99-1087
OWOW99-1412
OWOW99-1486
OWOW99-1537
OWOW99-1562
OWOW99-0007
OWOW99-0056
OWOW99-0107
OWOW99-0203
OWOW99-0206
OWOW99-0457
OWOW99-0531
OWOW99-0582
OWOW99-0628
Long. DD
-71.4065
-80.1384
-79.1741
-80.9912
-80.9015
-80.1292
-82.5805
-80.7032
-81.3848
-80.2755
-81.4667
-81.6083
-81.6246
-79.2482
-83.1017
-81.9264
-80.8089
-98.0560
-102.2547
-97.1801
-101.3716
-99.8458
-96.4624
-103.2273
-97.5077
-103.8945
Lat. DD
41.9839
34.5133
34.3024
33.2641
33.6414
34.3049
34.6606
34.3094
33.6076
33.5042
34.0877
33.8239
34.8750
33.3649
34.5784
34.8039
34.4193
43.7564
45.7699
44.8678
44.1562
45.7071
44.4955
45.1155
45.6993
44.2735
Lake Name
Arnolds Mills Reservoir
L MURRAY
HARTWELL RES
Lake Wateree
St.
Rl
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SD
SD
SD
SD
SD
SD
SD
SD
SD
County
PROVIDENCE
CHESTERFIELD
DILLON
BAMBERG
CALHOUN
DARLINGTON
ANDERSON
KERSHAW
AIKEN
CLARENDON
NEWBERRY
AIKEN
CHEROKEE
GEORGETOWN
OCONEE
SPARTANBURG
KERSHAW
DAVISON
PERKINS
CODINGTON
HAAKON
CAMPBELL
BROOKINGS
BUTTE
MARSHALL
LAWRENCE
Lake
Area
(ha)
6
3
159
37
18
36
3
2
18
3
19602
6
6
4
6881
3
5548
284
959
1124
5
6
616
4
83
681
Yr.
4
1
1
1
1
2
2
2
3
3
3
3
3
4
4
4
4
1
1
1
1
1
2
2
2
2
Longitude
Deg
71
80
79
80
80
80
82
80
81
80
81
81
81
79
83
81
80
98
102
97
101
99
96
103
97
103
Min
24
8
10
59
54
7
34
42
23
16
28
36
37
14
6
55
48
3
15
10
22
50
27
13
30
53
Sec
23.40
18.24
26.76
28.32
5.40
45.12
49.80
11.52
5.28
31.80
0.12
29.88
28.56
53.52
6.12
35.04
32.04
21.60
16.92
48.36
17.76
44.88
44.64
38.28
27.72
40.20
Latitude
Deg
41
34
34
33
33
34
34
34
33
33
34
33
34
33
34
34
34
43
45
44
44
45
44
45
45
44
Min
59
30
18
15
38
18
39
18
36
30
5
49
52
21
34
48
25
45
46
52
9
42
29
6
41
16
Sec
2.04
47.88
8.64
50.76
29.04
17.64
38.16
33.84
27.36
15.12
15.72
26.04
30.00
53.64
42.24
14.04
9.48
23.04
11.64
4.08
22.32
25.56
43.80
55.80
57.48
24.60
27 of 35
-------
OWOW99 Site-ID
OWOW99-0631
OWOW99-0653
OWOW99-0657
OWOW99-0928
OWOW99-0931
OWOW99-0982
OWOW99-1007
OWOW99-1031
OWOW99-1056
OWOW99-1107
OWOW99-1407
OWOW99-1453
OWOW99-1481
OWOW99-1507
OWOW99-1553
OWOW99-1556
OWOW99-1557
OWOW99-0087
OWOW99-0187
OWOW99-0487
OWOW99-0561
OWOW99-0587
OWOW99-0597
OWOW99-1036
OWOW99-1086
OWOW99-1487
Long. DD
-103.4072
-100.0315
-99.3955
-103.4528
-99.1439
-101.0124
-97.2311
-98.2920
-100.5331
-97.5925
-97.1505
-103.7237
-102.1920
-97.4514
-103.4179
-97.2857
-99.7300
-86.5603
-83.8330
-85.2748
-88.4150
-86.7644
-89.3677
-88.0792
-84.7785
-83.3651
Lat. DD
45.8542
43.3437
44.6185
44.8720
45.9093
44.3660
45.1795
43.4149
44.8741
44.4791
44.1469
45.0254
45.2425
45.3803
43.3078
45.7157
43.9121
36.0991
36.3113
36.5651
35.5581
36.4128
35.2595
36.4316
35.6188
35.9973
Lake Name
OAHE RES
J PERCY PRIEST L
Morris Lake
Dale Hollow Lake
KENTUCKY L
DOUGLAS L
St.
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
SD
TN
TN
TN
TN
TN
TN
TN
TN
TN
County
HARDING
TRIPP
HYDE
BUTTE
MCPHERSON
STANLEY
DAY
DOUGLAS
DEWEY
KINGSBURY
LAKE
BUTTE
PERKINS
DAY
FALL RIVER
MARSHALL
LYMAN
DAVIDSON
UNION
CLAY
HENDERSON
ROBERTSON
FAYETTE
HENRY
MEIGS
JEFFERSON
Lake
Area
(ha)
9
3
3
5
129
25
83
38
61520
119
95
7
43
940
1742
54
3
5370
3749
10726
184
5
5
46342
2
11139
Yr.
2
2
2
3
3
3
3
3
3
3
4
4
4
4
4
4
4
1
1
2
2
2
2
3
3
4
Longitude
Deg
103
100
99
103
99
101
97
98
100
97
97
103
102
97
103
97
99
86
83
85
88
86
89
88
84
83
Min
24
1
23
27
8
0
13
17
31
35
9
43
11
27
25
17
43
33
49
16
24
45
22
4
46
21
Sec
25.92
53.40
43.80
10.08
38.04
44.64
51.96
31.20
59.16
33.00
1.80
25.32
31.20
5.04
4.44
8.52
48.00
37.08
58.80
29.28
54.00
51.84
3.72
45.12
42.60
54.36
Latitude
Deg
45
43
44
44
45
44
45
43
44
44
44
45
45
45
43
45
43
36
36
36
35
36
35
36
35
35
Min
51
20
37
52
54
21
10
24
52
28
8
1
14
22
18
42
54
5
18
33
33
24
15
25
37
59
Sec
15.12
37.32
6.60
19.20
33.48
57.60
46.20
53.64
26.76
44.76
48.84
31.44
33.00
49.08
28.08
56.52
43.56
56.76
40.68
54.36
29.16
46.08
34.20
53.76
7.68
50.28
28 of 35
-------
OWOW99 Site-ID
OWOW99-1512
OWOW99-1536
OWOW99-0020
OWOW99-0021
OWOW99-0046
OWOW99-0048
OWOW99-0070
OWOW99-0073
OWOW99-0096
OWOW99-0120
OWOW99-0121
OWOW99-0123
OWOW99-0145
OWOW99-0148
OWOW99-0170
OWOW99-0195
OWOW99-0196
OWOW99-0198
OWOW99-0220
OWOW99-0221
OWOW99-0223
OWOW99-0470
OWOW99-0471
OWOW99-0473
OWOW99-0495
OWOW99-0496
Long. DD
-86.4277
-84.2180
-95.3874
-100.5777
-97.4428
-98.3790
-98.0258
-94.6013
-97.8593
-97.3485
-99.2766
-97.1985
-96.2522
-94.7224
-100.9665
-94.9041
-99.6494
-95.6121
-95.6834
-97.9284
-96.0107
-98.1155
-99.5140
-96.7899
-100.3492
-96.8272
Lat. DD
36.0389
35.6086
30.1851
31.9371
31.9740
33.7103
30.4154
32.8300
32.0622
26.2842
33.9259
33.0425
29.4629
33.2888
32.4319
29.8449
28.9065
33.4843
31.1694
28.2013
32.9492
26.1452
32.0370
33.8561
34.4613
32.0145
Lake Name
Tellico Lake
Roaers Lake
E V Spence Reservoir
Lake Arrowhead
Lake Travis
Flaa Branch Lake
Unnamed lake
Santa Rosa Lake
Bullock Lake
Lake Corpus Christ!
Lake Tawakoni
Lake Coleman
L TEXOMA
Lake Childress
Unnamed lake
St.
TN
TN
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
County
RUTHERFORD
MONROE
MONTGOMERY
COKE
BOSQUE
CLAY
TRAVIS
MARION
BOSQUE
CAMERON
WILBARGER
DENTON
WHARTON
MORRIS
MITCHELL
CHAMBERS
ZAVALA
LAMAR
HOUSTON
LIVE OAK
HUNT
HIDALGO
COLEMAN
GRAYSON
CHILDRESS
NAVARRO
Lake
Area
(ha)
714
6639
9
6055
2208
6561
7240
557
11
35
660
380
2
1239
2
4
5
6
23
7831
15333
8
705
23549
121
12
Yr.
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
Longitude
Deg
86
84
95
100
97
98
98
94
97
97
99
97
96
94
100
94
99
95
95
97
96
98
99
96
100
96
Min
25
13
23
34
26
22
1
36
51
20
16
11
15
43
57
54
38
36
41
55
0
6
30
47
20
49
Sec
39.72
4.80
14.64
39.72
34.08
44.40
32.88
4.68
33.48
54.60
35.76
54.60
7.92
20.64
59.40
14.76
57.84
43.56
0.24
42.24
38.52
55.80
50.40
23.64
57.12
37.92
Latitude
Deg
36
35
30
31
31
33
30
32
32
26
33
33
29
33
32
29
28
33
31
28
32
26
32
33
34
32
Min
2
36
11
56
58
42
24
49
3
17
55
2
27
17
25
50
54
29
10
12
56
8
2
51
27
0
Sec
20.04
30.96
6.36
13.56
26.40
37.08
55.44
48.00
43.92
3.12
33.24
33.00
46.44
19.68
54.84
41.64
23.40
3.48
9.84
4.68
57.12
42.72
13.20
21.96
40.68
52.20
29 of 35
-------
OWOW99 Site-ID
OWOW99-0520
OWOW99-0524
OWOW99-0545
OWOW99-0548
OWOW99-0570
OWOW99-0573
OWOW99-0596
OWOW99-0598
OWOW99-0620
OWOW99-0645
OWOW99-0646
OWOW99-0648
OWOW99-0670
OWOW99-0671
OWOW99-0673
OWOW99-0920
OWOW99-0921
OWOW99-0945
OWOW99-0946
OWOW99-0948
OWOW99-0970
OWOW99-0973
OWOW99-0974
OWOW99-0995
OWOW99-0996
OWOW99-0998
Long. DD
-96.0261
-94.1918
-95.0674
-94.3946
-98.2822
-95.5984
-99.0068
-96.6693
-98.3474
-97.6087
-98.4200
-96.6480
-94.8510
-97.8103
-95.4881
-101.8108
-97.5738
-95.9137
-99.1748
-96.5444
-96.6798
-94.3321
-94.1659
-96.2954
-101.1158
-96.0390
Lat. DD
28.7684
30.8490
29.5604
32.2129
32.6120
33.8270
32.7753
33.3078
29.8943
31.0062
28.3815
32.6450
30.4974
28.1089
32.1860
35.4638
31.1665
31.2285
27.9740
33.1304
28.3294
33.2844
32.0277
30.1495
30.1436
32.0818
Lake Name
B A Steinhaqen Lake
Clear Lake
Shelby Lake
Lake Pat Mayse
HUBBARDCRRES
ASCS Lake Riser 638
Stillhouse Hollow Lake
L PALESTINE
Lake Belton
Unnamed lake
Lake Lavon
Wriqht Patman Lake
Sabine River overflow
St.
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
County
MATAGORDA
HARRIS
PANOLA
PALO PINTO
LAMAR
STEPHENS
COLLIN
COMAL
BELL
MCMULLEN
DALLAS
POLK
SAN PATRICIO
HENDERSON
POTTER
BELL
LEON
WEBB
COLLIN
CALHOUN
BOWIE
PANOLA
WASHINGTON
VAL VERDE
HENDERSON
Lake
Area
(ha)
18
5549
812
5
7
2390
5960
7
19
2664
6
6
6
7
9533
655
1052
2
20
81
2
11360
5
2
51
10
Yr.
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
3
3
3
3
3
3
3
3
3
3
3
Longitude
Deg
96
94
95
94
98
95
99
96
98
97
98
96
94
97
95
101
97
95
99
96
96
94
94
96
101
96
Min
1
11
4
23
16
35
0
40
20
36
25
38
51
48
29
48
34
54
10
32
40
19
9
17
6
2
Sec
33.96
30.59
2.64
40.56
55.92
54.24
24.48
9.48
50.64
31.32
12.00
52.80
3.60
37.08
17.16
38.88
25.68
49.32
29.28
39.84
47.28
55.56
57.24
43.44
56.88
20.40
Latitude
Deg
28
30
29
32
32
33
32
33
29
31
28
32
30
28
32
35
31
31
27
33
28
33
32
30
30
32
Min
46
50
33
12
36
49
46
18
53
0
22
38
29
6
11
27
9
13
58
7
19
17
1
8
8
4
Sec
6.24
56.29
37.44
46.44
43.20
37.20
31.08
28.08
39.48
22.32
53.40
42.00
50.64
32.04
9.60
49.68
59.40
42.60
26.40
49.44
45.84
3.84
39.72
58.20
36.96
54.48
30 of 35
-------
OWOW99 Site-ID
OWOW99-1021
OWOW99-1045
OWOW99-1049
OWOW99-1070
OWOW99-1071
OWOW99-1073
OWOW99-1094
OWOW99-1095
OWOW99-1098
OWOW99-1120
OWOW99-1121
OWOW99-1370
OWOW99-1373
OWOW99-1395
OWOW99-1399
OWOW99-1420
OWOW99-1421
OWOW99-1446
OWOW99-1470
OWOW99-1471
OWOW99-1473
OWOW99-1495
OWOW99-1496
OWOW99-1498
OWOW99-1521
OWOW99-1523
Long. DD
-98.6771
-98.5051
-94.5522
-95.3163
-100.9560
-95.5320
-101.6812
-98.0244
-95.5161
-97.8470
-98.9015
-96.8215
-94.1256
-98.0162
-93.7712
-99.2401
-99.2335
-96.2172
-96.5598
-98.6921
-96.9868
-95.5053
-100.1961
-96.3628
-100.3713
-97.6110
Lat. DD
33.3955
32.0189
31.5542
28.9617
29.6535
33.1012
35.6553
30.3621
32.5682
27.1988
33.7473
32.2412
32.7491
28.9365
31.5230
26.8182
31.3159
31.9798
30.7472
28.6395
33.1494
29.6160
28.5733
33.1896
33.0911
33.4887
Lake Name
Lake Proctor
CADDO L
Toledo Bend Reservoir
Richland Reservoir
Lake Lewisville
What Is It Pond
St.
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
TX
County
YOUNG
COMANCHE
NACOGDOCHES
BRAZORIA
VAL VERDE
HOPKINS
MOORE
TRAVIS
SMITH
KENEDY
ARCHER
ELLIS
MARION
KARNES
SABINE
ZAPATA
MCCULLOCH
ROBERTSON
ATASCOSA
DENTON
FORT BEND
MAVERICK
COLLIN
STONEWALL
MONTAGUE
Lake
Area
(ha)
9
1913
3
2
588
5
593
225
6
5
3
9
10794
8
67141
116
6
18124
2
1
8590
1
3
9
7
5
Yr.
3
3
3
3
3
3
3
3
3
3
3
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
Longitude
Deg
98
98
94
95
100
95
101
98
95
97
98
96
94
98
93
99
99
96
96
98
96
95
100
96
100
97
Min
40
30
33
18
57
31
40
1
30
50
54
49
7
0
46
14
14
13
33
41
59
30
11
21
22
36
Sec
37.56
18.36
7.92
58.68
21.60
55.20
52.32
27.84
57.96
49.20
5.40
17.40
32.16
58.32
16.32
24.36
0.60
1.92
35.28
31.56
12.48
19.08
45.96
46.08
16.68
39.60
Latitude
Deg
33
32
31
28
29
33
35
30
32
27
33
32
32
28
31
26
31
31
30
28
33
29
28
33
33
33
Min
23
1
33
57
39
6
39
21
34
11
44
14
44
56
31
49
18
58
44
38
8
36
34
11
5
29
Sec
43.80
8.04
15.12
42.12
12.60
4.32
19.08
43.56
5.52
55.68
50.28
28.32
56.76
11.40
22.80
5.52
57.24
47.14
49.92
22.20
57.84
57.60
23.88
22.56
27.96
19.32
31 of 35
-------
OWOW99 Site-ID
OWOW99-1545
OWOW99-1570
OWOW99-1571
OWOW99-1573
OWOW99-0102
OWOW99-0144
OWOW99-0152
OWOW99-0526
OWOW99-0626
OWOW99-0927
OWOW99-1027
OWOW99-1051
OWOW99-1352
OWOW99-1476
OWOW99-0064
OWOW99-0090
OWOW99-0512
OWOW99-0514
OWOW99-0614
OWOW99-0914
OWOW99-0964
OWOW99-1039
OWOW99-1089
OWOW99-1364
OWOW99-1462
OWOW99-1539
Long. DD
-97.3344
-95.5857
-99.3188
-95.5930
-113.7755
-110.8572
-110.2791
-111.8377
-112.6432
-111.8883
-110.1109
-111.1449
-110.1033
-111.8073
-77.8438
-77.3122
-79.0903
-78.5318
-77.3104
-77.7039
-76.5704
-75.9664
-78.9541
-76.3460
-79.4531
-77.6024
Lat. DD
30.0141
30.4678
26.9216
32.6230
37.2618
37.3143
40.6966
39.0707
40.8812
41.8315
40.7634
40.1871
40.3998
40.2025
38.0644
37.9672
36.6820
36.5703
37.4233
38.8207
36.8671
37.4214
36.7873
37.0230
36.7772
37.2620
Lake Name
Lake Conroe
INTERNATIONAL
FALCON RES
Strawberry Reservoir
Utah Lake
Lake Anna
unnamed
Big Lake
Griggs Pond
unnamed
Lone Star Lake
Banister Lake
unnamed pond
Lake Chesdin
St.
TX
TX
TX
TX
UT
UT
UT
UT
UT
UT
UT
UT
UT
UT
VA
VA
VA
VA
VA
VA
VA
VA
VA
VA
VA
VA
County
BASTROP
MONTGOMERY
ZAPATA
SMITH
WASHINGTON
KANE
DUCHESNE
SAN PETE
TOOELE
CACHE
DUCHESNE
WASATCH
DUCHESNE
LOUISA
CAROLINE
HALIFAX
MECKLENBURG
HENRICO
PRINCE WILLIAM
SUFFOLK
NORTHAMPTON
HALIFAX
HAMPTON
PITTSYLVANIA
CHESTERFIELD
Lake
Area
(ha)
4
8030
15802
3
101
912
4
15
921
7
10
3172
2
0
5254
11
10
10
6
3
13
628
154
170
1
1316
Yr.
4
4
4
4
1
1
1
2
2
3
3
3
4
4
1
1
2
2
2
3
3
3
3
4
4
4
Longitude
Deg
97
95
99
95
113
110
110
111
112
111
110
111
110
111
77
77
79
78
77
77
76
75
78
76
79
77
Min
20
35
19
35
46
51
16
50
38
53
6
8
6
48
50
18
5
31
18
42
34
57
57
20
27
36
Sec
3.84
8.52
7.68
34.80
31.80
25.92
44.76
15.72
35.52
17.88
39.24
41.64
11.88
26.21
37.68
43.92
25.08
54.48
37.44
14.04
13.44
59.04
14.76
45.60
11.16
8.64
Latitude
Deg
30
30
26
32
37
37
40
39
40
41
40
40
40
40
38
37
36
36
37
38
36
37
36
37
36
37
Min
0
28
55
37
15
18
41
4
52
49
45
11
23
12
3
58
40
34
25
49
52
25
47
1
46
15
Sec
50.76
4.08
17.76
22.80
42.48
51.48
47.76
14.52
52.32
53.40
48.24
13.56
59.28
8.86
51.84
1.92
55.20
13.08
23.88
14.52
1.56
17.04
14.28
22.80
37.92
43.20
32 of 35
-------
OWOW99 Site-ID
OWOW99-0093
OWOW99-0942
OWOW99-1042
OWOW99-1092
OWOW99-1467
OWOW99-0004
OWOW99-0080
OWOW99-0154
OWOW99-0179
OWOW99-0202
OWOW99-0204
OWOW99-0504
OWOW99-0529
OWOW99-0654
OWOW99-0954
OWOW99-0979
OWOW99-1054
OWOW99-1354
OWOW99-1379
OWOW99-1479
OWOW99-1554
OWOW99-0008
OWOW99-0058
OWOW99-0059
OWOW99-0108
OWOW99-0133
Long. DD
-72.8915
-72.0592
-72.4259
-73.2782
-72.3081
-121.3595
-117.5813
-120.9586
-119.5883
-123.7674
-122.8397
-120.3321
-121.1618
-121.3833
-119.4764
-122.4597
-119.3222
-117.2925
-118.8874
-118.9817
-121.6659
-89.2323
-92.2221
-89.0859
-92.6510
-90.9843
Lat. DD
42.8282
44.7480
43.6486
44.8277
44.8707
47.3342
47.7677
48.5632
46.9819
48.0848
46.9928
48.0261
46.6403
47.5843
48.9145
48.0215
46.9868
48.4300
48.0631
46.0048
47.6052
44.0659
45.7970
46.0610
45.2943
45.8117
Lake Name
Lake Whitingham
Lake Willouqhby
Lake Pinneo
Kidder Pond
Keechelus Lake
Dry Lake
Unnamed/Vulcan
Frenchman Hills Lake
Cresent Lake
unnamed
Lake Chelan
Rimrock Lake
Lake Dorothy
Mud Lake
Lone Lake
Potholes Reservoir
Buffalo Lake
LakeWallula
Calligan Lake
Iroqami (Fish) Lake
Warner Lake
Noseeum Lake
Osceola Lake
Lake Winter
St.
vr
vr
vr
vr
vr
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
WA
Wl
Wl
Wl
Wl
Wl
County
WINDHAM
ORLEANS
WINDSOR
GRAND ISLE
ORLEANS
KITTITAS
SPOKANE
SKAGIT
GRANT
CLALLAM
THURSTON
CHELAN
YAKIMA
KING
OKANOGAN
ISLAND
GRANT
PEND OREILLE
OKANOGAN
BENTON
KING
WAUSHARA
BURNETT
VILAS
POLK
SAWYER
Lake
Area
(ha)
1565
670
7
3406
6
955
5
3
138
1995
6
13091
952
102
4
34
11333
936
226
12961
117
116
71
5
17
110
Yr.
1
3
3
3
4
1
1
1
1
1
1
2
2
2
3
3
3
4
4
4
4
1
1
1
1
1
Longitude
Deg
72
72
72
73
72
121
117
120
119
123
122
120
121
121
119
122
119
117
118
118
121
89
92
89
92
90
Min
53
3
25
16
18
21
34
57
35
46
50
19
9
22
28
27
19
17
53
58
39
13
13
5
39
59
Sec
29.40
33.12
33.24
41.52
29.16
34.06
52.61
30.82
17.77
2.71
22.88
55.38
42.44
59.88
34.97
34.81
19.99
33.07
14.50
54.16
57.17
56.28
19.56
9.24
3.60
3.48
Latitude
Deg
42
44
43
44
44
47
47
48
46
48
46
48
46
47
48
48
46
48
48
46
47
44
45
46
45
45
Min
49
44
38
49
52
20
46
33
58
5
59
1
38
35
54
1
59
25
3
0
36
3
47
3
17
48
Sec
41.52
52.80
54.96
39.72
14.52
2.94
3.58
47.63
54.88
5.32
34.19
33.96
25.08
3.41
52.24
17.47
12.48
48.00
47.02
17.21
18.54
57.24
49.20
39.60
39.48
42.12
33 of 35
-------
OWOW99 Site-ID
OWOW99-0134
OWOW99-0158
OWOW99-0208
OWOW99-0458
OWOW99-0508
OWOW99-0533
OWOW99-0565
OWOW99-0608
OWOW99-0658
OWOW99-0666
OWOW99-0958
OWOW99-0959
OWOW99-0983
OWOW99-0991
OWOW99-1033
OWOW99-1058
OWOW99-1059
OWOW99-1083
OWOW99-1084
OWOW99-1108
OWOW99-1365
OWOW99-1383
OWOW99-1408
OWOW99-1433
Long. DD
-89.5894
-90.5273
-89.6991
-89.9860
-89.5351
-91.5771
-91.1565
-90.1691
-89.6562
-88.4157
-91.3394
-89.3112
-89.1146
-89.0375
-91.2826
-91.8659
-89.1653
-92.3092
-89.5527
-91.3828
-90.8791
-89.8021
-90.4251
-92.6653
Lat. DD
45.8619
46.2912
44.8103
43.9352
45.6967
45.7591
43.3500
46.0857
45.2854
44.0020
46.1505
45.8418
44.5307
43.9647
46.3544
45.4135
45.5817
45.6438
46.1376
45.2325
43.1321
45.9586
44.1284
45.4906
Lake Name
Sweeney Lake
Maki Lake
L DUBAY/Big Eau
Pleine Res
Castle Lake
Bob's Lake
unnamed
Turtle Flambeau Flowage
Black Alder Lake
Lake Winnebago
Pacwawong Lake
Boa Lake
Hatch Lake
unnamed
Flynn Lake
Yellow River Reservoir
Mars (Seguilla) Lake
Crooked Lake
Big Gibson Lake
Calkins North Lake
unnamed
Bolton Lake
Unnamed I
Beede Lake
St.
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
Wl
County
ONEIDA
IRON
MARATHON
JUNEAU
ONEIDA
WASHBURN
CRAWFORD
LINCOLN
WINNEBAGO
SAWYER
ONEIDA
WAUPACA
GREEN LAKE
BAYFIELD
BARRON
ONEIDA
POLK
VILAS
CHIPPEWA
CRAWFORD
VILAS
MONROE
POLK
Lake
Area
(ha)
78
17
5356
5010
8
4
59
0
5
53757
76
20
46
7
27
21
16
8
48
5
5
57
6
2
Yr.
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
3
3
3
4
4
4
4
Longitude
Deg
89
90
89
89
89
91
91
90
89
88
91
89
89
89
91
91
89
92
89
91
90
89
90
92
Min
35
31
41
59
32
34
9
10
39
24
20
18
6
2
16
51
9
18
33
22
52
48
25
39
Sec
21.84
38.28
56.76
9.60
6.36
37.56
23.40
8.72
22.32
56.52
21.84
40.32
52.56
15.00
57.36
57.24
55.08
33.12
9.72
58.08
44.76
7.56
30.36
55.08
Latitude
Deg
45
46
44
43
45
45
43
46
45
44
46
45
44
43
46
45
45
45
46
45
43
45
44
45
Min
51
17
48
56
41
45
21
5
17
0
9
50
31
57
21
24
34
38
8
13
7
57
7
29
Sec
42.84
28.32
37.08
6.72
48.12
32.76
0.00
8.52
7.44
7.20
1.80
30.48
50.52
52.92
15.84
48.60
54.12
37.68
15.36
57.00
55.56
30.96
42.24
26.16
34 of 35
-------
OWOW99 Site-ID
OWOW99-1483
OWOW99-1484
OWOW99-1533
OWOW99-1558
OWOW99-1566
OWOW99-0637
OWOW99-0052
OWOW99-0502
OWOW99-0527
OWOW99-0528
OWOW99-0602
OWOW99-1052
OWOW99-1053
OWOW99-1077
OWOW99-1078
OWOW99-1377
OWOW99-1477
OWOW99-1478
OWOW99-1503
OWOW99-1527
Long. DD
-92.2409
-88.2877
-91.2291
-88.8840
-88.3072
-80.8542
-109.3330
-106.9291
-109.3050
-109.2586
-110.6277
-110.3633
-106.2468
-109.6685
-110.3662
-106.2790
-109.3615
-106.7534
-106.1244
-110.8467
Lat. DD
45.9427
45.2655
45.5345
45.1419
43.0729
38.2409
43.0084
42.6746
42.8726
44.4925
44.2998
43.4642
43.1947
43.3312
44.4549
41.3601
42.8049
44.4843
42.7497
43.8054
Lake Name
Upper Loon Lake
(Phernetton)
Mirror Lake
McGee Lake
Pewaukee Lake
Summersville Lake
Yellowstone Lake
St.
Wl
Wl
Wl
Wl
Wl
wv
WY
WY
WY
WY
WY
WY
WY
WY
WY
WY
WY
WY
WY
WY
County
BURNETT
MARINETTE
RUSK
LANGLADE
WAUKESHA
NICHOLAS
FREMONT
NATRONA
FREMONT
PARK
TETON
TETON
NATRONA
FREMONT
ALBANY
SUBLETTE
JOHNSON
NATRONA
TETON
Lake
Area
(ha)
24
4
1617
8
985
844
4
1
73
1385
1116
35
24
40
0
5
3
821
8
4
Yr.
4
4
4
4
4
2
1
2
2
2
2
3
3
3
3
4
4
4
4
4
Longitude
Deg
92
88
91
88
88
80
109
106
109
109
110
110
106
109
110
106
109
106
106
110
Min
14
17
13
53
18
51
19
55
18
15
37
21
14
40
21
16
21
45
7
50
Sec
27.24
15.72
44.76
2.40
25.92
15.12
58.80
44.76
18.00
30.96
39.72
47.88
48.48
6.60
58.43
44.40
41.40
12.24
27.84
48.12
Latitude
Deg
45
45
45
45
43
38
43
42
42
44
44
43
43
43
44
41
42
44
42
43
Min
56
15
32
8
4
14
0
40
52
29
17
27
11
19
27
21
48
29
44
48
Sec
33.72
55.80
4.20
30.84
22.44
27.24
30.24
28.56
21.36
33.00
59.28
51.12
40.92
52.32
17.53
36.36
17.64
3.48
58.92
19.44
35 of 35
-------
-------
Appendix B
Standard Operating Procedure:
Fish Tissue Sample Collection Procedures for a
National Study of Chemical Residues in
Lake Fish Tissue
-------
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 1 of 9
Standard Operating Procedure
1444444444444444444444444444444444
Fish Tissue Sample Collection Procedures for a National Study of
Chemical Residues in Lake Fish Tissue
444444444444444444444444444444444444444444444444444444444444444444444444444444
Scope and Applicability: This Standard Operating Procedure (SOP) must be followed by all Field
Sample Collection Teams involved with the USEPA Office of Water's National Study of Chemical
Residues in Lake Fish Tissue. Adherence to the SOP will ensure that field sampling activities will be
performed the same way every time, i.e., are standardized, for all sampling participants.
Fish tissue sample collection procedures are presented below as sequential steps, and include specific
equipment, materials, and methods required to perform field sampling activities only.
Responsibility and Personnel Qualifications: This procedure may be used by any Field Sampling
Teams that have been authorized by the USEPA Project Manager or the USEPA Regional/State/ Tribal
Fish Sampling Coordinators to collect fish for the National Study of Chemical Residues in Lake Fish
Tissue.
References: U.S. Environmental Protection Agency (USEPA). 1995. Guidance for Assessing
Chemical Contaminant Data for Use in Fish Advisories. Volume 1: Fish Sampling and
Analysis. Second Edition. U.S. Environmental Protection Agency, Office of Water,
Washington, D.C. EPA 823-R-95-007.
U.S. Environmental Protection Agency (USEPA). 1997. Quality Assurance Project
Plan for the Cook Inlet Contaminant Study Sampling. U.S. Environmental Protection
Agency, Office of Water, Office of Science and Technology, Washington, D.C.
U.S. Environmental Protection Agency (USEPA). 1997. Sampling Plan for Conducting
Field Sampling and Chemical Analysis for the Cook Inlet Contaminant Study. U.S.
Environmental Protection Agency, Office of Water, Office of Science and Technology,
Washington, D.C.
U.S. Environmental Protection Agency (USEPA). 1999a. EPA Requirements for
Quality Assurance Project Plans. U.S. Environmental Protection Agency, Quality
Assurance Division, Washington, D.C. Interim Final. EPA QA/R-5.
U.S. Environmental Protection Agency (USEPA). 1999b. National Study of Chemical
Residues in Lake Fish Tissue: Study Design. U.S. Environmental Protection Agency,
Office of Water, Office of Science and Technology, Washington, D.C.
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 2 of 9
Precautions: Follow usual safety precautions for working in the field. Boats and/or electrofishing
equipment should only be operated by qualified, experienced operators trained in their proper use. Each
vessel must be equipped with the appropriate Coast Guard-required safety equipment (including personal
floatation devices for each field team member). If electrofishing equipment is used for sample collection,
each team member must be insulated from the water, boat, and electrodes via rubber boots and gloves.
Equipment/Materials:
Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment)(a)
Electrofishing equipment - OPTIONAL (including variable voltage pulsator unit, generator, electrodes,
wiring cables, dip nets, protective gloves, protective boots, and all necessary safety equipment)(a)
Nets - OPTIONAL (including trawls, seines, gill nets, fyke nets, trammel nets, hoop nets, pound nets,
trap nets)(a)
Angling equipment - OPTIONAL (including fishing rods, reels, line, terminal tackle, trot lines/3-1
Coast Guard-approved personal floatation devices
Maps of target lakes and access routes
Global Positioning System (GPS) unit - OPTIONAL (a)
pH meter (including associated calibration supplies)(a)
Livewell and/or buckets
Measuring board (millimeter scale)
Ice chests(b)
Aluminum foil (solvent-rinsed and baked)(b)
Heavy-duty food grade polyethylene tubing(b)
Large plastic (composite) bags(b)
Knife or scissors
Clean nitrile gloves(b)
Field Record Forms(b)
Sample Identification Labels(b)
Chain-of-Custody Forms(b)
Chain-of-Custody Labels(b)
Scientific collection permit
Dry Ice(b)
Black ballpoint pens and/or waterproof markers
Clipboard
Packing/strapping tape
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 3 of 9
Overnight courier airbills(b)
Plastic cable ties(b)
Plastic bubble-wrap(b)
First aid kit and emergency telephone numbers
(a) Selection and exact specifications at the discretion of the experienced on-site fisheries biologist.
Provided by the sample control center.
Procedures:
1. Identify the target lake to be sampled using the USEPA Office of Water's Target Lake List.
Locate the target lake via the coordinates provided in the Target Lake List and USGS
topographic maps (or equivalent maps).
2. Based on site reconnaissance, determine whether the target lake meets the definition of a suitable
lake for sampling for the purposes of this study, i.e., each lake must:
be a permanent body of water of at least one hectare in surface area,
have a minimum of 1,000 m2 of open (unvegetated) water,
be greater than 1 meter deep, and
have a permanent fish population (e.g., no annual winterkill, not recently stocked with
young fish).
If the target lake meets all of the above criteria, and if in the case of private property, the
landowner allows access/permission to sample the lake, proceed with Step 3. If the lake does not
meet the definition of a lake and/or if a private landowner denies access, record the problem and
contact the USEPA Project Manager and/or the Terra Tech Task Leader.
3. Assemble an array of both active and passive gear types, to ensure the collection of the desired
target numbers and species of fish. Selection of the most appropriate gear type(s) for a particular
target lake will be at the discretion of the experienced on-site fisheries biologist. Detailed
procedures for use or deployment of all possible gear types are not included here. However, if
passive collection devices (e.g., gill nets) are used, they must be checked frequently (e.g., several
times daily if possible, but at least every 24 hours) to ensure a limited time lag between fish
entrapment and sample preparation. Sampling Teams must be qualified, experienced, and/or
trained on the safe and effective use of each gear type selected.
4. Sampling gear will be selected and deployed to obtain samples of both predator species and
bottom-dwelling species. Suggested target species, listed in order of preference, are as follows:
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 4 of 9
11
If
I Ł
1 2
E I
Ł 1
S Ł
Family name
Centrarchidae
Percidae
Percichthyidae
Esocidae
Salmonidae
Cyprinidae
Ictaluridae
Catostomidae
Common name
Largemouth bass
Smallmouth bass
Black crappie
White crappie
Walleye
Yellow perch
White bass
Northern pike
Lake trout
Brown trout
Rainbow trout
Brook trout
Common carp
Channel catfish
Blue catfish
Brown bullhead
Yellow bullhead
White sucker
Scientific name
Micropterus salmoides
Micropterus dolomieu
Pomoxis nigromaculatus
Pomoxis annularis
Stizostedion vitreum
Percaflavescens
Morone chrysops
Esox lucius
Salvelinus namaycush
Salmo trutta
Oncorhynchus mykiss
Salvelinus fontinalis
Cyprinus carpio
Ictalurus punctatus
Ictalurus furcatus
Ameiurus nebulosus
Ameiurus natalis
Catostomus commersoni
5. As soon as fish have been obtained via active collection methods (or removed from passive
collection devices) they must be identified to species. Clean nitrile gloves must be worn during
the sample handling process. Potential target species/individuals will be rinsed in ambient water
to remove any foreign material from the external surface and placed in clean holding containers
(e.g., livewells, buckets). Nontarget fishes or small specimens are returned to the lake.
6. One predator and one bottom-dwelling species composite will be retained from each target lake.
Each composite must consist of five fish of adequate size (i.e., adult specimens that collectively
will provide greater than 560 grams of edible tissue for predators and 560 grams of total body
tissue for bottom dwellers) for analysis. Select fish for each composite based on the following
criteria:
all are of the same species,
all satisfy legal requirements of harvestable size (or weight), or at least be of consumable
size if no legal harvest requirements are in effect,
all are of similar size, so that the smallest individual in a composite is no less than 75% of
the total length of the largest individual, and
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 5 of 9
all are collected at the same time, i.e., collected as close to the same time as possible, but
no more than one week apart (Note: Individual fish may have to be frozen until all fish to
be included in the composite are available for delivery to the sample preparation
laboratory).
Accurate taxonomic identification is essential in assuring and defining the organisms that have
been composited and submitted for analysis. Under no circumstances should individuals from
different species be used in a single composite sample.
7. Following selection of five fish for each of the two composites that meet the above-listed criteria
for compositing, measure each to determine total body length. Measure total length of each
specimen in millimeters, from the anterior-most part of the fish to the tip of the longest caudal
finray (when the lobes of the caudal fin are depressed dorsoventrally).
8. Record species retained, specimen length, location collected and sampling date and time on the
Field Record Form (Figure 1) in black ink. Complete site location description portions of the form,
and draw a simple sketch of the sampling area in the space provided. One Field Record Form
will be completed for each composite collected from the target lake.
9. Assign the unique ten-character composite sample ID number to each composite as directed on
the Field Record Form (Figure 1):
state of collection (two-character abbreviation),
year of collection (two-number abbreviation),
lake identification number (four-digit code from Appendix A),
composite type (one character P = predator species; B = bottom-dwelling species), and
sample type (one character S = standard sample; D = duplicate sample).
10. Sign and date the Field Record Form.
11. Remove each fish retained for analysis from the clean holding container(s) (e.g., livewell) using
clean nitrile gloves. Dispatch each fish using a clean wooden bat (or equivalent wooden device).
12. Wrap each fish in extra heavy-duty aluminum foil (provided by the sample control center as
solvent-rinsed, oven-baked sheets).
13. Prepare a Sample Identification Label (Figure 2) (in black ink) for each sample, ensuring that the
label information matches the information recorded on the Field Record Form.
14. Cut a length of food grade tubing (provided by sample control center) that is long enough to
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 6 of 9
contain each individual fish and to allow extra length on each end to secure with cable ties.
Place each foil-wrapped specimen into the appropriate length of tubing. Seal each end of the
tubing with a plastic cable tie, and attach the appropriate Sample Identification Label.
15. Double-bag each entire specimen package, that is, place inside a large plastic bag with all
specimens of the same species from that site and seal with another cable tie.
16. As soon as each sample is packaged, place it immediately on dry ice for shipment. If samples
will be carried back to a laboratory or other facility to be frozen before shipment, wet ice can be
used to transport wrapped and bagged fish samples in the coolers to a laboratory or other interim
facility.
17. If possible, keep all (five) specimens designated for a particular composite in the same shipping
container (ice chest) for transport.
18. Samples may be stored on dry ice for a maximum of 24 hours. Sampling teams have the option,
depending on site logistics, of:
shipping the samples packed on dry ice in sufficient quantities to keep samples frozen for
up to 48 hours, via priority overnight delivery service (e.g., Federal Express), so that they
arrive at the sample preparation laboratory within less than 24 hours from the time of
sample collection, or
freezing the samples within 24 hours of collection at <-20°C, and storing the frozen
samples until shipment within 1 week of sample collection (frozen samples will
subsequently be packed on dry ice and shipped to the sample preparation laboratory via
priority overnight delivery service).
19. Complete a Chain-of-Custody Form (Figure 3). All entries must be in black ink and coincide with
specimen/sample information on the Sample Identification Labels and Field Record Forms.
20. Retain one copy of the Chain-of-Custody Form and Field Record Form, place and seal all other
copies in a waterproof bag, and enclose the sealed forms in the shipping container (ice chest).
21. Pack each shipping container (completely) with dry ice, secure each container with packaging
tape, and seal it (e.g., across the latch of the ice chest) with a Chain-of-Custody Label (provided
by the sample control center). Include the signature of the sampler and the date/time sealed (in
black ink) on each Chain-of-Custody Label.
22. Ship each container to the laboratory via priority overnight express delivery service, as directed by
the USEPA Project Manager or Terra Tech Task Leader. Monitor sample holding time, and
factor time required for shipment/delivery to ensure that the preservation and holding criteria
described in Step 18 have been met.
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 7 of 9
Figure 1. Field record for fish samples
Field Record for National Study of Chemical Residues in Lake Fish Tissue
Composite Sample ID:
(State) (Year) (Lake ID Number) (Composite type (Sample type
Sampling Date :
Collection Method(s):.
P=predator or 3=standard or
B=bottom-dweller) D=duplicate)
Collector Name (print and sign):
Affiliation:
Address:
Phone:
Site Location
Latitude:
County:
Longitude:.
Site Name:
Site Description:.
Circle one: natural lake, modified natural lake, human-made reservoir, other.
Estimated maximum lake depth meters
pH surface mid (optional)
bottom (optional).
Sample Description
Fish Species:
Specimen
#
Length
(mm)*
Location
Total Number of Individuals:
Date/Time Notes
01
02
03
04
05
Additional Comments:
*minimum individual size should be no less than 75% maximum individual size
Sampling Site Diagram
FORM DISTRIBUTION: White - Tetra Tech Task Leader Yellow & Pink - Sample Prep Lab Gold - Sampler
EPA Sample Number (to be assigned by Prep Lab)'.
-------
Figure 2. Sample identification label(a).
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 8 of 9
1
Project Name
Site Identification
Specimen #
Composite Sample ID #
Date
Time
Preservative
Collected by
(a) See Appendix D for key to complete Sample Identification Label.
-------
SOP Fish Tissue Sample Collection
Final
Date: 22 May 2000
Page 9 of 9
FigureS. Chain-of-Custody Form.
Q
CC
O
O
Q
O
1
o
re
re
o>
(A
re
o
'5)
Ł
o
m
o
c
o
0)
0)
Shaded area for Tt use only:
Type of Analyses Requested
iz
_i
o
o
"o.
1 0
(S) Q
H
d.
Cond/Salinity
Chlorine
Appearance
Log Number
O T3
GŁ
i-s
S5
SJ9UIB1UOD 10 JSdlUnN
(M/JO 8AI1BAJ8S8.M
ent Contact:
Project Manager or Cl
Address/Phone:
Contact Name/Phone:
oi
E
ro
-G
'2
D.
(D
J2
-z.
CL
Sample Location:
O
D)
ro
D.
Sample Identification/Station
E
i-
1o
Q
Date/Time:
Received by:
(signature)
Date/Time:
Relinquished by:
(signature)
Date/Time:
Sampled by:
(signature)
Date/Time:
Received by:
(signature)
Date/Time:
Received by:
(signature)
Date/Time:
Received by:
(signature)
Q.
(0
-e
o
Q.
Ol
CD
O
H
CQ
Dd
(f)
Q
O
reproduced electronically
-------
-------
Appendix C
Standard Operating Procedure:
pH Measurements
-------
-------
SOP pH Measurements
Final
Date: 22 May 2000
Page 1 of 3
Standard Operating Procedure
pH Measurement Procedures for a National Study of Chemical
Residues in Lake Fish Tissue
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Scope and Applicability: This Standard Operating Procedure (SOP) must be followed by all Field
Sample Collection Teams involved with the USEPA Office of Water's National Study of Chemical
Residues in Lake Fish Tissue. Adherence to the SOP will ensure that field pH measurements will be
performed the same way every time, i.e., are standardized, for all sampling participants.
Procedures for field pH measurement are presented as sequential steps in the SOP to follow, and include
equipment, materials, and methods required to perform field measurements only.
Responsibility and Personnel Qualifications: This procedure may be used by any Field Sampling
Teams that have been authorized by the USEPA Project Manager or the USEPA Regional/State/ Tribal
Fish Sampling Coordinators to collect fish for the National Study of Chemical Residues in Lake Fish
Tissue.
References: American Public Health Association (APHA). 1995. Part 4500 Ff: pH Value.
Pages 4-65 - 4-69 in APHA. Standard Methods for the Examination of Water and
Wastewater. APHA, Washington, D.C.
Precautions: Follow usual safety precautions for working in the field. Boats should only be operated by
qualified, experienced operators trained in their proper use. Each vessel must be equipped with the
appropriate Coast Guard-required safety equipment (including personal floatation devices for each field
team member). Caution must be used when deploying pH probes overboard, and deployment should only
occur when vessels are stable and not under power.
Equipment/Materials:
Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment)(a)
Coast Guard-approved personal floatation devices
Maps of target lakes and access routes
Field Record Forms(b)
Clipboard
pH meter with pH electrode/probe(a)
Standard buffer solutions (pH 4, 7, and 10)
Calibration cup or beaker
Distilled water
Calibration log book(a)
(a) Selection and exact specifications at the discretion of the experienced on-site fisheries biologist.
Provided by the sample control center.
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SOP pH Measurements
Final
Date: 22 May 2000
Page 2 of 3
Procedures:
GENERAL
Procedures provided herein focus on pH probe deployment and pH measurement, not specific component
assembly or operation since selection of a particular meter for use is at the discretion of the individual
field teams. Field teams are urged to read, understand, and follow the manufacturer's instructions for
their pH meter of choice for this study.
CALIBRATION
1. Prior to deployment (i.e., at a minimum once daily during field use), the pH meter must be
calibrated as per manufacturer's specifications. Calibration procedures presented here are
generalized and are not water-specific.
2. Rinse the calibration cup (or beaker) and pH sensor with distilled, deionized water.
3. Pour off the deionized water and fill the calibration cup with pH 4.0 buffer solution, making sure
that the pH sensor is completely immersed in buffer solution.
4. Turn the meter display on and monitor pH. When the pH reading has stabilized to a consistent
value, note the reading in a calibration log book or field notebook.
5. If the displayed value is different from the buffer/standard, adjust the meter (e.g., via a
calibration setting) to match the buffer value. Record the final calibrated pH reading (which
must match the buffer value) in the calibration log book.
6. Decant the 4.0 buffer solution from the calibration cup, and rinse the pH sensor and calibration
cup with distilled water.
7. Fill the calibration cup with 7.0 buffer solution and repeat Steps 4 through 5.
8. Decant the pH 7.0 buffer solution and rinse the pH sensor and calibration cup with distilled
water. Fill the calibration cup with pH 10.0 buffer solution and repeat Steps 4 through 5.
9. Decant the buffer solution and rinse the pH sensor with distilled water.
10. Once the meter is successfully calibrated, lake pH can be measured. Water sample temperature
must be similar in temperature to that of the calibration standards (or use and adjust the
temperature compensation feature of the meter).
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SOP pH Measurements
Final
Date: 22 May 2000
Page 3 of 3
DEPLOYMENT AND SAMPLE MEASUREMENT
11. Deploy the pH sensor by gently lowering the probe into the water. Be sure that the sensor is
completely immersed.
Specific deployment and measurement instructions for the National Fish Tissue Study requires
only one measurement location (with an optional vertical profile) per target lake, and are as
follows:
a. In waters < 2 meters deep, measure pH at approximately 30 cm (approximately 1 ft)
below the surface.
b. In waters > 2 meters deep, measure pH at approximately 30 cm below the surface, at
mid-depth, and at 30 cm above lake bottom substrates; however if multiple measurements
or vertical profiles are not feasible (e.g., due to lake depth, meter probe cord length, etc.),
record a single measurement at 30 cm below the surface.
12. When the pH reading has stabilized to a consistent value, record the reading on the Field Record
Form (provided by the sample control center) to the nearest 0.1 unit.
13. Repeat Step 12 for mid-depth and near bottom measurements, as appropriate and if feasible
(refer to Step 11).
14. Follow manufacturer's instructions for pH meter and electrode storage and maintenance.
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Appendix D
Field Data Element Dictionary
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Field Data Element Definitions and Instructions
Associated with Field Sample Collection Activities for the
National Study of Chemical Residues in Lake Fish Tissue
Element
Affiliation:
The Affiliation field (on the Field Record Form) contains the
agency, group, or company name of those persons conducting
the field effort.
Collected by:
Collection Method:
Collector Name:
Composite Sample ID:
EPA Sample Number
Estimated Maximum Lake Depth:
Fish Species:
The Collected by field on the Sample Identification Label is
synonymous with the Collector Name field on the Field Record
Form, and contains the name of the Field Team Leader.
The Collection Method field on the Field Record Form
contains the listing of sampling gear types used to collect
samples.
The Collector Name field on the Field Record Form is
synonymous with the Collected by field on the Sample
Identification Label, and contains the name of the Field Team
Leader.
The Composite Sample ID field on the Field Record Form and
the Sample Identification Label is composed of a ten-character
code including state of collection (two-character abbreviation),
year of collection (two-number abbreviation), lake identification
number (four-digit USEPA code), composite type (one character
P = predator species, B = bottom-dwelling species), and
qualifier for standard or duplicate composite samples (one
character S = standard sample, D = duplicate sample).
Please leave this space blank on the Field Record Form. The
EPA Sample Number is an analytical tracking number that will
be assigned by the sample preparation laboratory to each
individual aliquot offish tissue prepared for analysis.
The Estimated Maximum Lake Depth field on the Field
Record Form stores a depth estimate in meters for the deepest
portion of the target lake. Estimates will be made by the Field
Team and may be based on sources such as lake maps, depth
sounder readings, anchor line lengths, etc.
The Fish Species field on the Field Record Form stores the
common name of the fish retained for analysis. Scientific name
entries are optional.
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Length:
Location:
Preservative:
Project Name:
Sampling Date:
Site Description:
Site Identification:
Site Name:
Specimen #:
Time:
The Length field on the Field Record Form contains the
individual Total Length (in millimeters) of each fish retained for
analysis. Total length of each specimen is measured from the
anterior-most part of the fish to the tip of the longest caudal
finray (when the lobes of the caudal fin are depressed
dorsoventrally) and recorded to the nearest mm.
The Location field on the Field Record Form stores a brief
description of the area in the lake where each fish was collected.
The Preservative field on the Sample Identification Label
stores information on how the samples were preserved for
shipment, i.e., either on dry ice or frozen.
The Project Name field on the Sample Identification Label
contains the designation "USEPA National Fish Tissue Study".
The Sampling Date field on the Field Record Form and Sample
Identification Label stores the numerical month/date/year (e.g.,
10/02/99) of sample collection.
The Site Description field on the Field Record Form contains a
brief written description of the location of the lake (e.g., road or
town landmarks) and area of lake sampled (e.g., east portion of
the lake).
The Site Identification field on the Sample Identification Label
is synonymous with the Site Name field on the Field Record
Form, and contains the lake name.
The Site Name field on the Field Record Form is synonymous
with the Site Identification field on the Sample Identification
Label and contains the lake name.
The Specimen # field on the Field Record Form and the Sample
Identification Label consists of a two-digit number from 01
through 05.
The Time field on the Sample Identification Label and Field
Record Form stores time of sample collection recorded in
military time (i.e., four digits). Time fields on the Field Record
Form include collection times for each individual specimen.
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