&EPA United States Environmental Protection Agency Office of Water 4305T Washington, DC 20460 EPA-823-R-02-005 Quality Assurance Project Plan for Sample Collection Activities for a National Study of Chemical Residues in Lake Fish Tissue Prepared/or: Leanne Stahl, National Study Manager U.S. Environmental Protection Agency, Office of Science and Technology 1200 Pennsylvania Avenue, NW (MC 4305T) Washington, D.C. 20460 Prepared by: Tetra Tech, Inc. 10045 Red Run Boulevard, Suite 110 Owings Mills, MD 21117 May 2000 ------- ------- Quality Assurance Project Plan for Sample Collection Activities for a National Study of Chemical Residues in Lake Fish Tissue Prepared/or: Leanne Stahl, Work Assignment Manager U.S. Environmental Protection Agency, Office of Science and Technology 1200 Pennsylvania Avenue, NW (MC 4305) Washington, D.C. 20460 Prepared by: Tetra Tech, Inc. 10045 Red Run Boulevard, Suite 110 Owings Mills, MD 21117 May 22, 2000 Final This quality assurance project plan (QAPP) has been prepared according to guidance provided in the document EPA Requirements for Quality Assurance Project Plans (EPA QA/R-5, U.S. Environmental Protection Agency, Quality Assurance Division, Washington, DC, Interim Final, November 1999) to ensure that environmental and related data collected, compiled, and/or generated for this project are complete, accurate, and of the type, quantity, and quality required for their intended use. The work conducted by Tetra Tech will be in conformance with the quality assurance program described in the quality management plan for Tetra Tech's Fairfax Group and with the procedures detailed in this QAPP. Approvals: Esther C. Peters, Ph.D. Quality Assurance Officer Tetra Tech, Inc. Date Elaine D. Snyder !Y Work Assignment Leader Tetra Tech, Inc. 'Date William Telliard Quality Assurance Manager USEPA, Office of Science & Technology Leanne Stahl Work Assignment Manager USEPA, Office of Science & Technology Date ------- ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page iii of xv CONTENTS List of Tables v List of Figures v Distribution List vi A Project Management 1.0 Project/Task Organization 1 2.0 Problem Definition/Background 4 3.0 Project/Task Description 6 4.0 Quality Objectives and Criteria for Measurement Data 9 4.1 Project Quality Objectives 9 4.2 Measurement Performance Criteria 10 5.0 Special Training Requirements/Certification 13 6.0 Documentation and Records 13 B Data Acquisition 7.0 Sampling Process Design 16 7.1 Sample Type 16 7.2 Sampling Period 17 7.3 Sample Frame 17 7.4 Selection of Lakes for Sampling 18 7.5 Nontarget Population, Inaccessible Lakes, and Lakes for Which Access is Denied 19 7.6 Reserve Sample of Lakes 20 8.0 Sampling Methods 21 8.1 Target Species 21 8.2 Composite Sampling 22 8.3 Sample Collection 23 9.0 Sample Handling and Custody Requirements 25 9.1 Sample Handling 25 9.2 Sample Integrity 26 9.3 Custody Requirements 27 ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page iv of xv 10.0 Analytical Methods Requirements 30 11.0 Quality Control Requirements 30 12.0 Instrument/Equipment Testing, Inspection, and Maintenance Requirements . 30 13.0 Instrument Calibration and Frequency 31 14.0 Inspection/Acceptance Requirements for Supplies and Consumables 31 15.0 Data Acquisition Requirements (Nondirect Measurements) 31 16.0 Data Management 31 C Assessment/Oversight 17.0 Assessment and Response Actions 32 18.0 Reports to Management 34 D Data Validation and Usability 19.0 Data Review, Validation, and Verification Requirements 34 20.0 Validation and Verification Methods 34 21.0 Reconciliation with Data Quality Objectives 35 Literature Cited 36 Appendices A Randomly Selected List of Target Lakes B Standard Operating Procedure: Fish Tissue Sample Collection Procedures for a National Study of Chemical Residues in Lake Fish Tissue C Standard Operating Procedure: pH Measurements D Field Data Element Dictionary ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page v of xv LIST OF TABLES Table 1 Project Time Line for Milestones Associated with Fish Tissue Sample Collection Activities 8 Table 2 Types of Field Data to Be Collected in Association with Fish Tissue Sample Collection 10 Table 3 Numbers of Lakes by Size Category in Sample Frame (Based on RF3) 18 Table 4 Number of Lakes Selected for Sampling by Size Category and Year 19 Table 5 Number of Lakes (by Size Category and Year) Selected as a Reserve Sample .... 20 Table 6 Recommended Target Species for Inland Freshwaters (in Order of Preference) 22 Table 7 Equipment and Supply List for Fish Tissue Sampling 24 LIST OF FIGURES Figure 1 Organizational Diagram for the National Study of Chemical Residues in Lake Fish Tissue 2 ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page vi of xv DISTRIBUTION LIST James Amrhein WI Department of Natural Resources P.O. Box 7921 Madison, WI 53707 608/266-5325 (phone) 608/266-2244 (fax) Tom Armitage USEPA/OST (4305) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-5388 (phone) 202/260-9830 (fax) Alan Auwarter EPA Region 4 Science and Ecosystem Support Division 980 College Station Road Athens, GA 30605-2720 706/355-8704 (phone) 706/355-8726 (fax) John Biagi GADNR Wildlife Resources Division The Stream Team 2425 Marbin Farms Road Mansfield, GA 30055 770/784-3126 (phone) 770-784-3129 (fax) William Botts PADEP 909 Elmerton Avenue Harrisburg,PA17110 717/705-4776 (phone) 717/705-4760 (fax) Mark A. Briggs MN Department of Natural Resources 500 Lafayette Road, Box 25 St. Paul, MN 55155-4025 651/215-0316 (phone) 651/296-1811 (fax) Robert Brodberg CA Environmental Protection Agency Office of Environmental Health Hazard Assessment 301 Capitol Mall, Rm 205 Sacramento, CA 95814-4327 916/323-4763 (phone) 916/327-7320 (fax) Alan Buchanan Missouri Department of Conservation 1110 S. College Ave. Columbia, MO 65201 573/882-9880 X3257 (phone) 573/882-4517 (fax) Lawrence Burkhard USEPA Environmental Effects Research Lab Mid Continent Ecology Division/ORD 6201 Congdon Boulevard Duluth, MN 55804 218/529-5164 (phone) 218/529-5003 (fax) Dan Butler OKCC 413 Northwest 12th Street Oklahoma City, OK 73103-3706 405/979-2206 (phone) 405/979-2212 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page vii of xv Michael Callam NE Department of Environmental Quality 1200 N Street, Suite 400 Atrium Bldg. Lincoln, NE 68509-8922 402/471-4249 (phone) 402/471-2909 (fax) Bud Cann NJDEP P.O. Box 427, 35 Arctic Parkway Trenton, NJ 08625 609/292-0427 (phone) 609/633-1095 (fax) Robert W. Cooner AL Dept. of Environmental Management P.O. Box 301463 Montgomery, AL 36130-1463 334/260-2746 (phone) 334/272-8131 (fax) Sarah Conrad DynCorp, Inc. 6101 Stevenson Avenue Alexandria, VA 22304 703/461-2373 (phone) Dave Courtemanch MEDEP State House #17 Augusta, ME 04333 207/287-7789 (phone) 207/287-7191 (fax) Dave Crane CA Department of Fish and Game Water Pollution Control Lab 2005 Nimbus Road Rancho Cordova, CA 95670 916/358-2859 (phone) 916/985-4301(fax) Steve Cringan KS Department of Health & Environment Division of Environment Forbes Field Topeka, KS 66620 785/296-5571 (phone) Phil Crocker USEPA, Region 6 Fountain Place/6WQ-EW 1445 Ross Avenue Suite 1200 Dallas, TX 75202-2733 214/665-6644 (phone) 214/665-6689 (fax) Marc Dahlberg Arizona Game and Fish Department 2221 W. Greenway Phoenix, AZ 85023 602/789-3260 (phone) 602/789-3265 (fax) Bob Day MI Department of Environmental Quality Surface Water Quality Division Knapps Centre P.O. Box 30273 Lansing, MI 48909-7773 517/335-3314 (phone) 517/373-9958 (fax) Richard Denton UT Department of Environmental Quality Division of Water Quality P.O. Box 144870 Salt Lake City, UT 84114-4870 801/538-6055 (phone) 801/538-6016 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page viii of xv Kevin Dorman AR Department of Environmental Quality P.O. Box 8912 Little Rock, AR 72219-8912 501/682-0666 (phone) 501/682-0910 (fax) Don Dycus Tennessee Valley Authority 1101 Market Street, CST17D Chattanooga, TN 37402 423/751-7322 (phone) 423/751-7648 (fax) Robert Estabrook NHDES 6 Hazen Drive Concord, NH 03301 603/271-3357 (phone) 603/271-7899 (fax) Mike Ell ND Department of Health Division of Water Quality 1200 Missouri Ave., Rm 203 Bismarck, ND 58506 701/328-5214 (phone) 701/328-5200 (fax) John Ford MO Department of Natural Resources P.O. Box 176 Jefferson City, MO 65102 573/751-7428 (phone) 573/751-7024 (fax) Gene Foster OR Department of Environmental Quality Water Quality Division 811 SW 6th Avenue Portland, OR 97204 503/229-5358 (phone) 503/229-6124 (fax) Robert Frey PA Department of Environmental Protection Bureau of Watershed Conservation P.O. Box 8555 Harrisburg, PA 17105-8555 7177787-9637 (phone) 717/787-9549 (fax) Mike George UtahDEQ 288 N, 1460 W Salt Lake City, UT 84114-4870 801/538-9325 (phone) 801/538-6016 (fax) Al Gibson MS DEQ, Field Services Division Biological Services Section 1542 Old Whirfield Road Pearl, MS 39208 601/664-3963 (phone) 601/664-3938 (fax) Jim Green US EPA Wheeling Field Office 1060 Chapline Street, Suite 303 Wheeling, WV 26003-2995 304/234-0243 (phone) 304/234-0260 (fax) Jean Gregory VA Department of Environmental Quality P.O. Box 10009 Richmond, VA 23240-0009 804/698-4113 (phone) Mark Hale NC DENR, Division of Water Quality Ecosystems Analysis Unit 4401 Reedy Creek Road Raleigh, NC 27607 919/733-6946 x240 (phone) 919/733-9959 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page ix of xv Robert Hanten SD Game, Fish, and Parks 523 East Capitol Street Pierre, SD 57501 605/223-7702 (phone) 605/773-3399 (fax) Scott Hardin FL Game & Freshwater Fish Commission Farris Bryant Building 620 S. Meridian Street Tallahassee, FL 32399-1600 850/488-4068 (phone) Chris Harman AL DEM, Montgomery Branch Field Operations Division 1890 Dickinson Drive, Building A Montgomery, AL 36109 334/260-2751 (phone) Linda Harn GA Department of Natural Resources Environmental Protection Division Watershed Planning & Monitoring Program 7 Martin Luther King Jr. Drive, Suite 643 Atlanta, GA 30330 404/656-4905 (phone) 404/657-7031 (fax) Janet Hashimoto USEPA, Region 9 75 Hawthorne Street San Francisco, CA 94105 415/744-1933 (phone) 415/744-1235 (fax) Brian Hoelscher IDDEQ 1410 N.Hilton Boise, ID 83706 208/3 73-0117 (phone) 208/373-0143 (fax) Thomas Hornshaw IL Environmental Protection Agency 1021 North Grand Avenue East Springfield, IL 62794-9276 217/785-0830 (phone) 217/782-1431 (fax) Bill Horton ID Department of Fish & Game P.O. Box 25 Boise, ID 83707-0025 208/334-3791 (phone) Peter Husby USEPA Region 9 Laboratory 1337 S. 46th Street Building 201 Richmond, CA 94804-4698 510/412-2331 (phone) Gary Isbell Ohio Department of Natural Resources Division of Wildlife Fountain Square, G-3 Columbus, OH 53224-1837 Rachel Jablonka USEPA, Region 2 290 Broadway New York, NY 10007-1866 212/637-3853 (phone) 212/637-3889 (fax) Matt Kadlec WA Department of Ecology Environmental Assessment Program P.O. Box 47600 Olympia, WA 98504-7600 360/407-6771 (phone) 360/407-6884 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page x of xv Henry Kahn USEPA/OST (4303) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-5408 (phone) 202/260-7185 (fax) Chuck Kanetsky USEPA, Region 3 1650 Arch Street/3ES 10 Philadelphia, PA 19103-2029 215/814-2735 (phone) 215/814-2782 (fax) Ronald Klauda MD Department of Natural Resources Tawes State Office Building, B-2 580 Taylor Avenue Annapolis, MD 21401 410/974-3782 (phone) 410/974-2680 (fax) Kellie Kubena USEPA, Region 10 1200 Sixth Avenue/OEA-095 Seattle, WA 98101 206/553-1904 (phone) 206/553-0119 (fax) James P. Kurtenbach EPADESA 2890 Woodbridge Avenue Edison, NJ 08837 732/321-6695 (phone) 732/321-6616 (fax) Richard Langdon VT DEC Lab 103 S. Main Street Waterbury, VT 05676 802/241-1379 (phone) 802/241-3008 (fax) Dore LaRosta EPADESA 2890 Woodbridge Avenue Edison, NJ 08837 732/321-6686 (phone) 732/321-6616 (fax) Robert Maietta MA Department of Environmental Protection Office of Watershed Management 627 Main Street (2nd Floor) Worcester, MA 01608 508/767-2793 (phone) 508/791-4131 (fax) Randy Manning GADNR Environmental Protection Division 745 Gaines School Road Athens, GA 30605 706/369-6376 (phone) 706/369-6398 (fax) Dave McKinney Tennessee Wildlife Resources Agency P.O. Box 40747 Nashville, TN 37204 615/781-6500 (phone) Chris Moore DynCorp, Inc. 6101 Stevenson Avenue Alexandria, VA 22304 703/461-2360 (phone) Barry Mower ME Department of Environmental Protection 17 Statehouse Station Augusta, ME 04333 207/287-7777 (phone) 207/287-7191 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page xi of xv Denise Moyer KY Department of Environmental Protection Division of Water Ecological Support Section 14 Reilly Road Frankfort, KY 40601 502/564-3401 XI56 (phone) 502/564-0111 (fax) Pete Nolan New England Regional Lab 60 Westview Street Lexington, MA 02421 781/860-4343 (phone) 781/860-4397 (fax) Kevin Novo-Gradac MD Department of the Environment 2500 Broening Hwy Baltimore, MD 21224 410/631-3 906 (phone) Scott Nykerk CO Division of Wildlife 6060 Broadway Denver, CO 80216 303/291-7323 (phone) 303/291-7456 (fax) Tony Olsen National Health and Environmental Effects Laboratory/ORD Western Ecology Division 200 SW 35th Street Corvallis, OR 97333-4902 541/754-4790 (phone) 541/754-4716 (fax) John Olson Iowa Department of Natural Resources Wallace State Office Building 900 E. Grand Avenue DesMoines, IA50319 515/281-8905 (phone) 515/281-8895 (fax) Toney Ott USEPA, Region 8 999 18th Street (8EPR-EP) Suite 500 Denver, CO 80202-2466 303/312-6909 (phone) Jimmie Overton NC DENR, Division of Water Quality Ecosystems Analysis Unit 4401 Reedy Creek Road Raleigh, NC 26707 919/733-9960 (phone) 919/733-9959 (fax) Randy Parham OKDEQ P.O. Box 1677 Oklahoma City, OK 73101-1677 405/702-1026 (phone) 405/702-1001 (fax) Steve Paulsen National Health and Environmental Effects Laboratory/ORD Western Ecology Division 200 SW 35th Street Corvallis, OR 97333-4902 541/754-4428 (phone) 541/754-4716 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page xii of xv Ken Paxton Ohio DNR 1840 Belcher Drive Columbus, OH 43221 614/265-6348 (phone) 614/262-1143 (fax) Eric Pearson ND Department of Health Division of Water Quality 1200 Missouri Ave., Rm 203 Bismarck, ND 58506 701/328-5230 (phone) 701/328-5200 (fax) John Persell Minnesota Chippewa Tribe P.O. Box 217 Cass Lake, MN 56633-0217 218/335-6303 (phone) 218/335-8187 (fax) Esther Peters Tetra Tech, Inc. 10306 Easton Place, Suite 340 Fairfax, VA 22030 703/385-6000 (phone) 703/385-6007 (fax) David Petrick TX Natural Resource Conservation Commission Standards and Assessment Section Water Quality Division P.O. Box 13087 Austin, TX 78711-3087 512/239-4598 (phone) 512/23 9-4420 (fax) Ernest Pizzuto CT Department of Environmental Protection Bureau of Water 79 Elm Street Hartford, CT 06106 860/424-3715 (phone) 860/424-4055 (fax) Tom Proch, PADEP/SWRO 400 Waterfront Dr. Pittsburgh, PA 15222 412/442-4051 (phone) 412/442-4325 (fax) Walter (Pete) Redmon USEPA Region 5 77 W. Jackson Blvd./WT15J Chicago, IL 60604-3507 312/886-6110 (phone) 312/886-0168 (fax) Amanda Richardson Tetra Tech, Inc. 10045 Red Run Blvd., Suite 110 Owings Mills, MD 21117 410/356-8993 (phone) 410/356-9005 (fax) Robert Richardson RIIDEM 235 Promenade Street Providence, RI 02908 401/222-4700 x7240 (phone) 401/521-4230 (fax) Lynn Riddick DynCorp, Inc. 6101 Stevenson Avenue Alexandria, VA 22304 703/461-2060 (phone) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page xiii of xv Bruce Ruppel NJ Department of Environmental Protection Division of Science and Research P.O. Box 409 Trenton, NJ 08625-409 609/984-6548 (phone) 609/292-7340 (fax) Gary Schiffmiller NMED 1190 St. Francis Drive Santa Fe, NM 87502 505/827-2470 (phone) 505/827-0160 (fax) Lorenzo Sena EPA Region 7 901 North 5th Street Kansas City, KS 66101 913/551-7017 (phone) 913/551-7948 (fax) Dave Serdar WADept. of Ecology 300 Desmond Drive Olympia, WA 98504-7600 360/407-6772 (phone) 360/407-6884 (fax) Tony Shaw PA DEP/NERO 400 Market Street Harrisburg, PA 17105 7177787-9637 (phone) 717/787-9549 (fax) Cindy Simbanin USEPA/OST (4303) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-5019 (phone) 202/260-7185 (fax) Don Skaar MT Department of Fish, Wildlife and Parks 1420 East 6th Avenue Helena, MT 59620-0701 406/444-5686, 406/444-2449 (phone) 406/444-4952 (fax) Larry Skinner NYS Dept of Environmental Conservation Bureau of Habitat 50 Wolf Road, Rm 576 Albany, NY 12233-4756 518/457-0751 (phone) 518/485-8424 (fax) Janice E. Smithson, Wildlife Biologist HI Department of Environmental Protection Division of Water Resources 1201 Greenbrier Street Charleston, WV 25311 Phone: (304) 558-2108 Fax: (304)558-2780 jsmithson@mail.dep.wv.state.us Elaine Snyder Tetra Tech, Inc. 10045 Red Run Blvd., Suite 110 Owings Mills, MD 21117 410/356-8993 (phone) 410/356-9005 (fax) Patrick Snyder Dept of Environment and Natural Resources 523 East Capitol Avenue Pierre, SD 57501 605/773-4729 (phone) Betsy Southerland USEPA/OST (4305) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-3966 (phone) 202/260-9830 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page xiv of xv JimStahl IN Department of Environmental Management OWM Shade 65-40-2 P.O. Box 6015 Indianapolis, IN 46206-6015 317/308-3187 (phone) 317/308-3219 (fax) Leanne Stahl USEPA/OST (4305) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-7055 (phone) 202/260-9830 (fax) Tom Stauffer PADEP/NERO 2 Public Square, Suite 151 Wilkes-Barre, PA 18711-0790 570/820-4923 (phone) 570/830-3016 (fax) Bill Telliard USEPA/OST (4303) 1200 Pennsylvania Avenue, NW Washington, DC 20460 202/260-7134 (phone) 202/260-7185 (fax) Will Tucker LADEQ 100 AsmaBlvd., Suite 151 Lafayette, LA 70508 318/262-5585 (phone) 318/262-5593 (fax) Dennis Unkenholz SD Department of Game, Fish & Parks 523 East Capitol Avenue Pierre, SD 57501 605/773-4508 (phone) Mark Warren NV Division of Wildlife 1100 Valley Road Reno, NV 89520 775/688-1535 (phone) 775/688-1595 (fax) Gary Welker EPA Region 7 901 N. 5th Street Kansas City, KS 66101 913/551-7177 (phone) 913/551-8752 (fax) Bob Williamson ILDNR 524 S. 2nd Street Springfield, IL 62701 217/782-6424 (phone) 217/785-8262 (fax) Sherry Wills PADEP 2 Public Square Wilkes-Barre, PA 18711-0790 570/826-2553 (phone) 570/830-3016 (fax) Steve Wolff Wyoming Game & Fish Department 5400 Bishop Blvd. Cheyenne, WY 82006 307/777-4673 (phone) 307/777-4677 (fax) John Woodling CO Division of Wildlife 6060 Broadway Denver, CO 80216 303/291-7224 (phone) 303/291-7456 (fax) ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page xv of xv Bill Yake WADept. of Ecology 300 Desmond Drive Olympia, WA 98504-7600 360/407-6778 (phone) 360/407-6884 (fax) Edward Younginer SCDHEC Bureau of Water Monitoring Assessment & Protection Division 2600 Bull Street Columbia, SC 29201 803/734-5401 (phone) 803/734-4435 (fax) ------- ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 1 of 36 A. PROJECT MANAGEMENT 1.0 PROJECT/TASK ORGANIZATION This Quality Assurance Project Plan (QAPP) describes the quality assurance (QA) and quality control (QC) activities/procedures that will be used while collecting samples for the National Study of Chemical Residues in Lake Fish Tissue (hereafter referred to as the National Fish Tissue Study) from 1999 through 2002. The purpose of this document is to present the methods and procedures that will be used for the collection offish tissue from lakes and reservoirs throughout the United States and the quality assurance procedures that will be employed. This document addresses only the sample collection effort of the National Fish Tissue Study. This QAPP was prepared according to guidance presented in the document EPA Requirements for Quality Assurance Project Plans, EPA QA/R-5 (USEPA 1999a). Reference to the QAPP elements described in the guidance document are included herein. The sample collection methods, procedures and protocols follow the guidelines and recommendations of Guidance For Assessing Chemical Contaminant Data For Use in Fish Advisories. Volume I: Fish Sampling and Analysis, Second Edition (USEPA 1995 )[or sub sequent updates]. The project team organization provides the framework for conducting the sample collection task to meet study objectives. The organizational structure and function also facilitate project performance and adherence to QC procedures and QA requirements. Key roles are filled by those persons responsible for ensuring the collection and processing of valid data and for routinely assessing the data for precision and accuracy, as well as the persons responsible for approving and accepting final products and deliverables. The project and QA personnel include staff from USEPA and other participating federal agencies, selected state resource agencies, Native American tribes, and Tetra Tech. The project organizational chart is presented in Figure 1, and includes relationships and lines of communication among key project team members. The USEPA Project Manager is Leanne Stahl, who will supervise the assigned project personnel to provide for their efficient utilization by directing their efforts either directly or indirectly. As Project Manager she will also have the following responsibilities: providing oversight for study design, site selection, and adherence to design objectives, reviewing and approving the project work plan, QAPP, and other materials developed to support the project, and coordinating with contractors, reviewers and USEPA Regions/States/Tribes to ensure technical quality and contract adherence. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 2 of 36 USEPA Office of Science and Technology Director William Telliard USEPA QA Manager Esther Peters Tetra Tech QA Officer Tetra Tech Field Sampling Team(s) Lea USEPA P Blaii Tetra Tec reject Manager 1 i : I ! 1 | ie Snyder USEPA State and Tribal Sampling Field Sampling Teams Teams USEPA Regional/State/Tribal Fish Sampling Coordinators Key Authority Lines of Communication Figure 1. Organizational Diagram for the National Study of Chemical Residues in Lake Fish Tissue. The USEPA Quality Assurance Manager is William Telliard, who will be responsible for reviewing and approving all Quality Assurance Project Plans (QAPPs). Additional USEPA QA Manager responsibilities include the following: reviewing and evaluating field procedures, conducting external performance and system audits of the procedures, and participating in Agency QA reviews of the study. The Tetra Tech Task Leader is Blaine Snyder, who will participate in study design and site selection processes. Other specific responsibilities of the Task Leader include the following: coordinating project assignments in establishing priorities and scheduling, ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 3 of 36 ensuring completion of high-quality projects within established budgets and time schedules, providing guidance, technical advice, and performance evaluations to those assigned to the project, implementing corrective actions and providing professional advice to staff, preparing and/or reviewing preparation of project deliverables, and providing support to USEPA in interacting with the project team (including the sample control center), technical reviewers, and USEPA Regions/States/Tribes to ensure technical quality requirements are met in accordance with project design objectives. The Tetra Tech Quality Assurance (QA) Officer is Esther Peters, whose primary responsibilities include the following: monitoring quality control (QC) activities to determine conformance, reviewing the QAPP for completeness and noting inconsistencies, providing support to USEPA and the Tetra Tech Task Leader in preparation of the work plan and QAPP and in their distribution, and approving the QAPP. The Regional Fish Sampling Coordinators or QA/QC Field Officers will be responsible for performing evaluations to ensure that QA/QC protocols are maintained throughout the sample collection and preparation processes. The evaluations will include reviewing all required documentation for completeness and seeing that any problems encountered outside normal operating conditions are documented and addressed, and verifying all other QA/QC procedures identified in the QAPP are followed. The USEPA Project Manager and the Tetra Tech Task Leader will coordinate and oversee the orientation of the Regional Fish Sampling Coordinators or QA/QC Field Officers responsible for USEPA Regional/State/Tribal Field Sampling Teams. Field Sampling Teams will be composed of: USEPA field staff, and/or State and Tribal field personnel, and/or Contractor-affiliated field staff (including subcontracted organizations or universities). ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 4 of 36 The Task Leader will direct and supervise the contractor-affiliated Field Sampling Teams and provide for their efficient utilization by directing their efforts. Both agency and contractor-affiliated field personnel are responsible for performing the field work, including collection, preparation, and shipment offish tissue samples and completion of field sampling records. The Field Sampling Teams will include scientific staff with specialization and technical competence in field sampling activities to effectively and efficiently perform the required work. They must perform all work in adherence with the project work plan and QAPP, including maintenance of sample custody and related documentation. Custody procedures are required to ensure the integrity of the samples with respect to prevention of contamination and maintenance of proper sample identification during handling. In this role, Field Sampling Teams are responsible for: receiving and inspecting the sample containers, completing and signing appropriate field records, assigning tracking numbers to each sample, verifying the completeness and accuracy of chain-of-custody documentation, controlling and monitoring access to samples while in their custody, and initiating shipment of the samples to appropriate destinations. 2.0 PROBLEM DEFINITION/BACKGROUND The USEPA Office of Water conducted a national screening-level investigation in 1987 (USEPA 1992) to determine the prevalence of selected bioaccumulative pollutants in fish and to correlate elevated fish tissue contaminant levels with pollutant sources. Gamefish and bottom-dwelling fishes were collected from 388 locations across the country thought to be influenced by various point and nonpoint sources. These fish tissue samples were analyzed to determine levels of 60 target analytes, including dioxins and furans, PCBs, pesticides and herbicides, mercury, and several other organic compounds. Results of the 1987 study indicated that target analytes were present in fish tissue at many of the sampling sites, and some of the contaminants (e.g., PCBs, dieldrin, mirex, and combined chlordane) occurred at levels posing potential human health risks. The Office of Science and Technology (OST) within the Office of Water has initiated work on a new four-year national study of chemical residues in fish tissue, which is designed to expand the scope of the 1987 study. In October 1998, USEPA convened a two-day workshop of more than 50 scientists from state, federal, and tribal agencies to obtain technical input on sampling design, target analytes, sampling methods and data management. Input from scientists at the workshop and other technical experts that participated in numerous study planning meetings was used to develop a final study design (USEPA 1999b). The contemporary study is statistically designed and will provide screening-level data on fish tissue contaminants from a greater number of waterbodies than were sampled in 1987. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 5 of 36 This study broadens the scope of the 1987 study (USEPA 1992) which focused on chemical residues in fish tissue near point source discharges. The new study will: provide information on the national distribution of selected persistent, bioaccumulative, and toxic (PBT) chemical residues in gamefish and bottom-dwelling fish in lakes and reservoirs of the coterminous United States (excluding the Great Lakes and the Great Salt Lake), include lakes and reservoirs selected according to a probability design, involve the collection offish from those randomly selected lakes and reservoirs over a four-year survey period (1999-2002), not be used to set fish consumption advisories; however, states and Native American tribes may choose to initiate a detailed fish study in a particular lake based on the screening contaminant concentrations provided by the national study, and include the analysis offish tissue for PBT chemicals selected from USEPA's multimedia candidate PBT list of 451 chemicals and from a list of 130 chemicals from several contemporary fish and bioaccumulation studies. A final target analyte list of 274 PBT chemicals (including breakdown products and PCB congeners) was compiled based on input from study design workshop participants and a review team of analytical experts convened in October 1998 and March 1999, respectively. Lakes and reservoirs were chosen as the target population because they: are accumulative environments where contamination is detectable, provide important sport fisheries nationwide, offer other recreational (non-fishing) access and opportunities, and occur in agricultural, urban, and less-developed areas, so that associations with each primary use may be determined. Lakes and reservoirs are the focus of this study rather than other waterbody types because: Fish consumption advisories represent 15.8% of the Nation's total lake acres (plus 100% of the Great Lakes), compared to 6.8% of the Nation's total river miles (USEPA 1999c). [Note: The Great Lakes will not be included in this study because substantial fish tissue contaminant information is available and continues to be collected in ongoing Great Lakes monitoring programs.] ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 6 of 36 Estuaries are currently being studied by USEPA's Environmental Monitoring and Assessment Program (EMAP). EMAP has sampled fish from East and Gulf Coast estuaries, and will include fish contamination in its Year 2000 initiative on West Coast estuaries. The specific objective of the new National Fish Tissue Study is to estimate the national distribution of the mean levels of selected persistent, bioaccumulative, and toxic chemical residues in fish tissue from lakes and reservoirs of the continental United States. In so doing, the study will provide the following types of information: information to meet objectives of the President's Clean Water Action Plan (CWAP) and to specifically respond to the following action item: CWAP Key Action #1: USEPA and NOAA will conduct a national survey of mercury and other contaminants in fish and shellfish throughout the country, and will coordinate the effort with states and tribes to maximize geographic coverage. The shellfish survey will be based on the data obtained by NOAA's ongoing Mussel Watch Project. information about persistent, bioaccumulative, and toxic chemicals (PBTs) for the Agency's PBT Initiative that addresses the following objective: The PBT Initiative seeks to identify areas of concern for human and/or ecological health. Study offish tissue may reveal where PBTs not previously considered a problem are present at levels of concern. data to answer important questions concerning the national occurrence of fish tissue contamination, such as the following: What is the national extent of selected chemical contaminants in fish from lakes and reservoirs of the coterminous United States (excluding the Great Lakes)? Are contaminant levels in fish high enough to warrant further investigation? 3.0 PROJECT/TASK DESCRIPTION The study design reflects the study goal and objectives defined by USEPA. The study goal can be stated simply to determine the extent to which fish in waters of the United States are contaminated with persistent, bioaccumulative, and toxic chemicals (PBTs). The project field sampling task presented and discussed in this document involves only those methods and ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 7 of 36 procedures used to collect and ship fish tissue samples for the National Fish Tissue Study. The Analytical Activities QAPP for the National Fish Tissue Study discusses the following study topics and tasks: sample preparation, compositing and homogenization; target analytes; analytical methods; and sample analysis. In consultation with the USEPA Office of Science and Technology, Tetra Tech will coordinate with USEPA headquarters and regional staff, state resource agencies, and Native American tribes to collect fish tissue samples from randomly selected lakes and reservoirs in the continental United States. With a combined network of partners and contractors, USEPA anticipates the sampling of approximately 500 lakes across the country (Appendix A) during the four-year sampling duration of the study (1999- 2002). The fish tissue samples will be collected based on a probability design to provide information on national distribution of the mean levels of contaminants in fish. This random selection of lakes and reservoirs is important for fulfilling the study design objectives, but adds complexity to field sampling logistics. Sampling Teams will need to be prepared to mobilize and sample fish from lakes in all parts of the country. The following elements will also add to the complexity of the field effort, and must be considered when planning field logistics: Field teams should consist of (at a minimum) one experienced fisheries biologist, one field technician, and a quality control specialist, all of whom must have experience with the array of fisheries sampling gear types to be used. In some cases the senior fisheries biologist may serve in dual capacities, assuming responsibility for site quality control (QC). The national study will include two groups of target fishes predator/gamefish and bottom-dwelling fish species (Section 8.1). Samples must consist of a composite offish (e.g., 5 individuals that will collectively provide greater than 560 grams of edible tissue for predators and 560 grams of total body tissue for bottom-dwellers) of the same target species and be the same relative size from each sampling location (Section 8.2). The optimum sampling window may be restricted due to biological, physical, and meteorological conditions and factors (Section 7.2). Each Sampling Team, in the combined network of samplers, will collect, prepare for shipment, and ship all fish tissue samples to a designated location according to the methods and procedures described in this QAPP and approved by the USEPA Project Manager. The USEPA Project Manager will be notified immediately by the Tetra Tech Task Leader and/or the USEPA Regional/State/Tribal Fish Sampling Coordinators of any problems related to successful completion of field efforts. Field sampling activities began in the fall of 1999, will continue in 2000 and 2001 during the summer and fall, and will conclude in the fall of 2002. Due to the effort required to initiate the ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 8 of 36 study (e.g., project coordination and development of study materials such as the study design document, QAPP, and training materials), fewer lakes were sampled in year one than will be sampled during other sampling years. In addition, some states indicated that they were unable to participate in sampling during the first year, but will have adequate time to prepare for sampling in subsequent years of the study. Data summaries for the four-year period of study and the final study report are scheduled to be completed in 2003. Implementation of the field sampling task will proceed with several milestones, as presented in Table 1. All activities associated with fish tissue sample collection will be conducted consistent with the requirements and procedures specified in this QAPP as approved by the USEPA Project Manager. Annual sampling activities will conclude with the development of a field collection effort summary (i.e., detailed listing of all sampling participants, sampling locations, and specimens collected) by Tetra Tech and reviewed by the USEPA Project Manager. The summaries will be used to document and report back to USEPA Regional/State/Tribal participants the collective sampling progress for each study year. Table 1. Project Time Line for Milestones Associated with Fish Tissue Sample Collection Activities. Activities and Milestones (1999 - 2002) Develop QAPP and project sampling plan for field efforts Conduct orientation for sampling and field QC personnel in EPA Regions Sampling of lakes and shipment of samples to analytical laboratory Field collection effort summaries and report-back to regions, states, and tribes ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 9 of 36 4.0 QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT DATA 4.1 Project Quality Objectives Data of known and documented quality are essential to the success of any monitoring or sampling program. Data quality objectives (DQOs) are qualitative and quantitative statements that clarify the intended use of the data, define the type of data needed to support the decision, identify the conditions under which the data should be collected, and specify tolerable limits on the probability of making a decision error due to uncertainty in the data. DQOs are developed by data users to specify the data quality needed to support specific decisions. Sources of error or uncertainty include the following: Sampling error: The difference between sample values and in situ true values from unknown biases due to collection methods and sampling design, Measurement error: The difference between sample values and in situ true values associated with the measurement process, Natural variation: Natural spatial heterogeneity and temporal variability in population abundance and distribution, and Error sources or biases associated with compositing, sample handling, storage, and preservation. This QAPP addresses only fish tissue sample collection activities, so the relevant quality objectives are primarily related to sample handling issues. One exception involves the measurement of lake pH. Study DQOs for pH will require that meters are calibrated to a known standard as per manufacturer's specifications (Appendix C). Types of field sampling data needed for this project are listed in Table 2. Discussion of conventional data quality indicators, i.e., precision, accuracy, completeness, representativeness, and comparability, follows in this section. Methods and procedures described in this document are intended to reduce the magnitude of the sources of uncertainty (and their frequency of occurrence) by applying the following approaches: use of standardized sample collection and handling procedures, and use of trained scientists to perform the sample collection and handling activities. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 10 of 36 Table 2. Types of Field Data to Be Collected in Association with Fish Tissue Sample Collection. Data Type Fish specimen Fish length Composite classification PH Estimated maximum lake depth Measurement Endpoint(s) or Units Species-level taxonomic identification Millimeters (mm), total length Predator or bottom-dwelling species nearest 0. 1 pH units Meters 4.2 Measurement Performance Criteria Measurement performance criteria are quantitative statistics that are used to interpret the degree of acceptability or utility of the data to the user. These criteria, also known as data quality indicators (DQIs), include the following: precision, accuracy, representativeness, completeness, and comparability. Precision Precision is a measure of internal method consistency. It is demonstrated by the degree of agreement between individual measurements (or values) of the same property of a sample, measured under similar conditions. As the analytical testing is beyond the scope of this QAPP, no specific criteria are required for this parameter. However, sufficient sample volumes (i.e., the five-fish composites described in Section 8.2) will be collected to allow for the assessment of precision during analytical laboratory testing. For this study, all fish in a lake cannot be sampled, and the laboratory analytical process is not perfect. The combined variability introduced by the sampling at a lake, the compositing offish, the subsampling of the composite for analysis, and the chemical analysis itself can be considered the "index" variability. The detection limits and analytical precision are one part of the analytical process that can be specified ahead of time (however analytical processes are not part of this QAPP). The orientation and training of sampling crews, and the process that they use to collect fish from a lake can also be standardized. Besides standardizing training, this dimension of variability cannot be reduced. The general rule of thumb is that if the combined index variability is less than 10% of the total variability, it will have little impact on the ability to estimate status. For this study the best way to develop an estimate of index variability is to simply revisit a subset, 10% of the sites, and repeat the ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 11 of 36 lake sampling procedure, compositing and analytical analyses. Sampling teams should plan to obtain duplicate fish samples from 10% of the target lakes and reservoirs in their state during the four-year study period. Accuracy Accuracy is defined as the degree of agreement between an observed value and an accepted reference or true value. For example, accuracy of pH meters used for this study will be assured through proper calibration to known standards, i.e., buffer solutions (Appendix C). Accuracy is a combination of random error (precision) and systematic error (bias), introduced during sampling and analytical operations. Bias is the systematic distortion of a measurement process that causes errors in one direction, so that the expected sample measurement is always greater or lesser to the same degree than the sample's true value. As mentioned previously, since analytical testing is beyond the scope of this QAPP, no accuracy criteria are identified here. However, proper sample handling procedures (Section 9.1) will be followed to minimize sample contamination. Representativeness Representativeness expresses the degree to which data accurately and precisely represent a characteristic of a population, parameter, variations at a sampling point, a process condition, or an environmental condition. Representativeness of the target species (Section 8.1) for this fish tissue sampling effort was established based on: the recommendation of USEPA's Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second Edition (USEPA 1995), the input from federal, state, and tribal scientists on the draft design of the National Study of Chemical Residues in Lake Fish Tissue, obtained primarily during the October 1998 workshop (USEPA 1999b), and approval by the USEPA Proj ect Manager. The representative goal for the sample collection effort will be satisfied by using experienced field biologists to ensure that the sample types and locations specified for the study are the samples actually collected. Completeness Completeness is defined as the percentage of measurements made that are judged to be valid according to specific criteria and entered into the data management system. To optimize completeness, every ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 12 of 36 effort is made to avoid sample and/or data loss. Accidents during sample transport or lab activities that cause the loss of the original samples will result in irreparable loss of data, which will reduce the ability to perform analyses, integrate results, and prepare reports. Samples will be stored and transported in unbreakable (plastic) containers (i.e., insulated ice chests). All sample processing (i.e., compositing, filleting, homogenization) will occur in a controlled environment within the laboratory, not in the field. The assignment of a set of specific sample numbers (Section 6.0) that have undergone chain-of-custody inspection makes it less likely for the sample preparation laboratory to overlook samples when preparing them for processing. Percent completeness (%C) for measurement parameters can be defined as follows: Where v = the number of measurements judged valid and T = the total number of measurements. Completeness, in the case of this project, is the number of valid samples collected relative to the number of samples that are planned to be collected. The completeness goal for this project is 90%. It should be noted that sample locations and numbers may change over the course of the four-year study, based on local conditions (e.g., accessibility of target lakes) and the availability of target fishes (e.g., natural biological abundance or distribution). Any and all changes must be approved by the USEPA Project Manager, and approved changes must be considered when assessing completeness. The completeness goal is achieved when 90% or more of the available samples from the final list of target lakes found to contain target fishes are collected and shipped with no errors in documentation or sample handling procedures. Comparability Comparability is an expression of the confidence with which one data set can be compared with another. Comparability is dependent on the proper design of the sampling program and on adherence to accepted sampling techniques, standard operating procedures, and quality assurance guidelines. For the fish tissue collection task, comparability of data will be accomplished by standardizing the sampling season, the field sampling methods, and the field training as follows: All samples will be collected during the late summer-fall (August-November). All samples will be collected and prepared for shipment according to standard operating procedures contained in this QAPP. These procedures are consistent with the recommendations of USEPA's Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second Edition (USEPA 1995 )[or sub sequent updates]. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 13 of 36 All field personnel involved with sampling will have adequate training and appropriate experience (Section 5.0). 5.0 SPECIAL TRAINING REQUIREMENTS/CERTIFICATION Each Field Sampling Team is required to have the necessary knowledge and experience to perform all field activities. This includes both knowledge and experience in the collection and identification of fishes, in the use of fisheries sampling gear specified for the study and in the operation of small boats. It also includes training in project-specific sample collection and handling procedures. The field sampling crews will be primarily composed of state, tribal, and regional fisheries biologists or contracted biologists with a strong technical background in fisheries sampling activities. Each Field Sampling Team should consist of (at a minimum) one experienced fisheries biologist, one field technician, and a quality control specialist, all of whom must have experience with the array of fisheries sampling gear types to be used. In some cases the senior fisheries biologist may serve in dual capacities, assuming responsibility for site quality control. This field sampling QAPP, the field sampling plan, and orientation materials will be distributed to all USEPA Regional/State/Tribal Fish Sampling Coordinators, who will, in turn, distribute it to all sampling personnel. Project orientation sessions will be set up by EPA Regions to distribute and discuss training materials. Materials will include detailed instructions for each field procedure (i.e., sampling of target fish, proper handling of the sample, shipping, and chain of custody) and visual training tools based on information from this QAPP. The focus of the orientation will be on sample collection methods, specific details of sample preparation, and strict adherence to the study's protocols. USEPA Regional/State/Tribal Fish Sampling Coordinators and Field Team Leaders will be required to view the training materials, read the QAPP, and verify in writing that they read or viewed the materials and understood the procedures and requirements. If sampling personnel change (i.e., new USEPA Regional/State/Tribal Fish Sampling Coordinators or new Field Team Leaders) during the course of the four-year study, the orientation process will have to be reinitiated for that particular new team. 6.0 DOCUMENTATION AND RECORDS Thorough documentation of all field sample collection and handling activities is necessary for proper processing in the laboratory and, ultimately, for the interpretation of study results. Field sample collection and handling will be documented in writing (for each sampling site) using the following forms and labels: a Field Record Form that contains information about each individual specimen and lake site (Appendix B), a Sample Identification Label that accompanies and identifies each sample (Appendix B), ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 14 of 36 a Chain-of-Custody Label that seals each sample container (provided by the sample control center), and a Chain-of-Custody Form that provides constant tracking information for all samples (Appendix B). A detailed description of each sample collected by each Field Sampling Team will be recorded on a Field Record Form (Appendix B). The form will document the sampling date, time, sampler's name, sampling site location/description, and sample description (count and length of each specimen). Also, the 10% subset of lakes and reservoirs that are sampled as duplicates will be noted as such on the form. The Field Record Form will also contain a unique tracking code (i.e., composite sample identification code) that will be used to identify each record. The ten-character code will include: state of collection (two-character abbreviation), year of collection (two-number abbreviation), lake identification number (four-digit code from Appendix A), composite type (one character P = predator species; B = bottom-dwelling species), and sample type (one character - S = standard sample, D = duplicate sample). The Field Record Form will be produced as a four-page carbonless copy form, with one copy retained by the sampler, and the other three included in the sample shipment to the laboratory (i.e., one for the sample preparation laboratory, one for the sample control center, and one for the Tetra Tech Task Leader). All entries will be made in ink and no erasures will be made. If an incorrect entry is made, the information will be crossed out with a single strike mark, which is initialed and dated by the sampler/recorder. A Sample Identification Label will be completed (Appendix B) to accompany each sample throughout the chain of custody. The label will document the project name, sampling site location, sampling date and time, the sampler's name, the ten-character tracking code, and the specimen number (e.g., 01 through 05). All entries will be made in indelible ink and will coincide with specimen and sample information on the Field Record Form. Descriptions and definitions of all field data elements required in the Field Record Form and Sample Identification Label are provided in Appendix D. Proper chain-of-custody procedures are necessary for tracking sample possession from field to laboratory. Chain-of-Custody Forms (Appendix B) will accompany each shipment of samples and will document sample identity (coinciding with information on the field record), sampler relinquishment date and time, and laboratory receipt date and time. Chain-of-Custody Forms will be produced as five- ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 15 of 36 page carbonless copies, with one copy for the sampler, and four for shipment to the laboratory (i.e., one for the sample preparation laboratory, one for the sample control center, one for the Tetra Tech Task Leader, and one for duplication and distribution to the analytical laboratories). Chain-of-Custody Labels will seal each sample container following packing operations in the field, and will include the signature of the sampler and the date and time sealed. All Chain-of-Custody Label and Form entries will be made in ink. Field sampling teams must notify the sample control center (DynCorp) by telephone (Chris Moore 703/461-2360 or Chris Maynard 703/461-2395) of an incoming shipment. Samples will be shipped from the field to the sample preparation laboratory via priority, overnight express delivery service. Copies of all shipping airbills will be retained by the sample control center. Specification for retention of field samples by the receiving location are outside the scope of this document. While in storage, it is recommended that samples held for analysis be stored with the original labeling materials. Annual sampling activities will conclude with the development of a field collection effort summary (i.e., detailed listing of all sampling participants, sampling locations, and specimens collected) by Tetra Tech and review of the summary by the USEPA Project Manager. Following USEPA Project Manager approval, the summaries will be used to document and report back to USEPA Regional/State/Tribal participants the collective sampling progress for each study year. Tetra Tech will maintain a file as a repository for information used in the preparation of the annual field collection summaries throughout the duration of the study. The following information will be included: any documents prepared for the study, contract and work assignment information, project QAPP, results of technical reviews, data quality assessments, and audits, communications (memoranda; internal notes; telephone conversation records; letters; meeting minutes; and all written correspondence between Tetra Tech, USEPA, and other project team personnel, subcontractors, suppliers, or others), maps, photographs, and drawings, and studies, reports, and documents pertaining to the project. If any change(s) in this QAPP is(are) required during the study, a memo will be sent to each person on the distribution list describing the change(s), following approval by the USEPA Project Manager. Any and all memos announcing changes must be attached to the QAPP. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 16 of 36 All documents and records prepared for this project will be maintained by USEPA and Tetra Tech during the project, and retained for a period of two years following completion of the project (unless otherwise directed by USEPA). B. DATA ACQUISITION 7.0 SAMPLING PROCESS DESIGN The objective of the National Fish Tissue Study is to estimate the national distribution of the mean levels of selected persistent, bioaccumulative, and toxic chemical residues in fish tissue from lakes and reservoirs of the continental United States. In so doing, the study will provide the following types of information: information to meet objectives of the President's Clean Water Action Plan (CWAP), information about persistent bioaccumulative toxic chemicals (PBTs) for the Agency's PBT Initiative, and data to answer important questions concerning the national occurrence of fish tissue contamination. For the purposes of this study design, the target population will be all lakes and reservoirs within the coterminous United States excluding the Laurentian Great Lakes and the Great Salt Lake. This study defines a lake as a permanent body of water of at least one hectare (2.47 acres) in surface area with a minimum of 1,000 m2 of open (unvegetated) water and a minimum depth of one meter. The lakes in this study must also have a permanent fish population. Approximately 500 locations will be sampled over the course of four years based on projections of available resources. 7.1 Sample Type To meet the study objectives, the National Fish Tissue Study will include composite sampling offish fillets for predator/gamefish species and whole fish for bottom-dwelling species from each sample lake. Five individuals per composite will be collected, all of which will be large enough to provide sufficient tissue for analysis of the group of target analytes. It has been determined that at least 560 grams of edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers will be required from the composites to allow for analysis of all target analytes. Based on the recommendations of USEPA's Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 17 of 36 Volume 1: Fish Sampling and Analysis, Second Edition (USEPA 1995), fish used in a composite sample must meet the following criteria: all be of the same species, satisfy any legal requirements of harvestable size or weight, or at least be of consumable size if no legal harvest requirements are in effect, be of similar size so that the smallest individual in a composite is no less than 75% of the total length of the largest individual, be collected at the same time (i.e., collected as close to the same time as possible but no more than 1 week apart) [Note: This assumes that a sampling crew was unable to collect all fish needed to prepare the composite sample on the same day. If organisms used in the same composite are collected on different days (no more than 1 week apart), individual fish will be frozen until all the fish to be included in the composite are available for delivery to the laboratory.], and be collected in sufficient numbers (five per composite) and of adequate size (five harvestable size adult specimens that collectively will provide greater than 560 grams of edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers) to allow analysis of recommended target analytes. Individual organisms used in composite samples must be of the same species because of notable differences in the species-specific bioaccumulation potential. Accurate taxonomic identification is essential in preventing the mixing of closely related species with the target species. Under no circumstance should individuals from different species be used in a composite sample. 7.2 Sampling Period Field sampling will be conducted during the period when water and weather conditions are conducive to safe and efficient field sampling, and when the target species are most frequently harvested by anglers. For most inland freshwaters, the most desirable sampling period is from late summer to early fall, since lipid content is usually highest and water levels are usually lowest at that time. Sampling should not occur during the spawning period of the particular target species being sought. With these recommendations in mind, and considering the geographic extent of the study area (i.e., range of latitudes and longitudes) the field sampling period will begin in August and last through November (and possibly into December in warmer regions). 7.3 Sample Frame For the purposes of this study, the target population will be all lakes and reservoirs within the coterminous United States excluding the Laurentian Great Lakes and the Great Salt Lake. For this ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 18 of 36 study, a lake is defined as a permanent body of water of at least one hectare (2.47 acres) in surface area with a minimum of 1,000 m2 of open (unvegetated) water, and a minimum depth of one meter. The lakes in this study must also have a permanent fish population. Examples of nonpermanent fish populations are lakes that are subject to annual fish winterkill, or are recently stocked with fingerlings. Stocked lakes with adult fish are defined as having a permanent fish population. The River Reach File Version 3 (RF3) was used to generate the list of lakes in the target population. RF3 constitutes the sample frame, and includes almost all lakes in the target population for this study. Noted exclusions are newly constructed reservoirs. However, RF3 is the best known national GIS coverage for lakes, so it was used in this study. To ensure the sample frame included all lakes and reservoirs with an area greater than 5,000 ha, a list from multiple sources of such lakes was constructed. The list was sent to USEPA Regional Offices and subsequently to each state to verify that each lake on the list was greater than 5,000 ha and to add any lakes greater than 5,000 ha that were not on the list. The corrected list of lakes was integrated into the RF3 list of lakes before sample selection was initiated. Table 3 summarizes the number of lakes in the sample frame used for sample selection. Table 3. Numbers of Lakes by Size Category in Sample Frame (Based on RF3). Lake area (ha) >l-5 >5-10 >10-50 >50-500 >500-5000 >5000 Number of Lakes 172,747 44,996 40,016 11,228 1,500 274 Frequency (%) 63.8 16.6 14.8 4.1 0.6 0.1 Cumulative Number of Lakes 172,747 217,743 257,759 268,987 270,387 270,761 Cumulative Frequency (%) 63.8 80.4 95.2 99.3 99.9 100.0 7.4 Selection of Lakes for Sampling The procedures described by Olsen et al. (1998) were used to select an unequal probability sample of lakes. The probability of selection for a lake depends on its area as given by RF3. In Table 4 the expected weight is the reciprocal of the probability of selection (inclusion probability). The inclusion probability was determined by the goal of obtaining approximately an equal number of lakes to sample in each size category. A higher percentage of the lakes in the smaller size categories would include lakes not meeting the target population definition of a lake. The probability of selection was adjusted so that the smaller size categories had a greater sample size. No adjustment was required for size categories 50-500 hectares, 500-5000 hectares, or > 5000 hectares. The adjustments for the remaining size categories were as follows: for 1-5 hectares, increase by 40%; for 5-10 hectares, increase by 30%; and for 10-50 hectares, increase by 20%. These adjustments were based on limited information from the EMAP northeastern lake survey. It is not known yet how well these will apply to ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 19 of 36 other regions of the country. The impact of an incorrect adjustment will be that the number of lakes actually sampled by size category will not be equal. Although it was not a requirement for the statistical survey design, study planners decided to select the sample by allocating the lakes to be sampled in each year of the study. It is recommended that the lakes be sampled in the year specified. The advantage of adhering to this approach is that if any year- to-year differences exist in fish tissue contaminants, then the sample will be balanced across years. In the event that the study must be stopped before all lakes can be sampled, sampling all lakes from a subset of the years (e.g., 1999-2001) results in a legitimate unequal probability sample of all lakes. The expected weights must be adjusted to account for the years not sampled. Table 4. Number of Lakes Selected for Sampling by Size Category and Year. Lake area (ha) >l-5 >5-10 >10-50 >50-500 >500-5000 >5000 Total 1999 39 44 32 34 36 40 225 2000 41 40 47 37 30 30 225 2001 47 47 46 29 31 25 225 2002 47 46 25 34 41 32 225 All Years 174 177 150 134 138 127 900 Expected Weight 938.84 261.61 256.51 85.06 11.36 2.21 7.5 Nontarget Population, Inaccessible Lakes, and Lakes for which Access is Denied A critical element of the statistical survey design is the determination of the status of each lake in the sample. This means that each lake is checked to determine if it meets the definition of a lake for the study (Section 7.3). In many cases, a field visit is not necessary to confirm that the lake meets the definition. In other cases, it may be necessary to actually visit the lake to determine if it meets the definition. Regardless, it is essential that a complete record of this information be reported to the USEPA Project Manager, since this information is required to complete the survey estimation procedures. Two other situations can occur that will result in a lake not being sampled. First, the lake may be on private land and require landowner permission to visit the lake. If a landowner refuses access to a lake selected for the study, then this needs to be recorded. Second, a lake may occasionally be physically inaccessible. If there are logistical or safety constraints that make a lake inaccessible, then the reason why the lake is inaccessible needs to be recorded and reported to the USEPA Project Manager and/or the Tetra Tech Task Leader. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 20 of 36 Information that must be determined during pre-sampling reconnaissance of each lake includes the following: Does the lake meet the definition of the target population (Section 7.3)? If the lake does not meet the definition, what are the reasons? For example: lake < 1 ha in surface area lake < 1 m depth lake < 1000 m2 of open water (unvegetated) saline lake with no fish population lake has no annual fish population (winterkill lake) other (list specific reasons) Has the landowner denied access to lake? (Record landowner information) Is the lake physically inaccessible during sampling period of study? If so, state why. 7.6 Reserve Sample of Lakes As a contingency, a second sample of lakes has been selected as a reserve. Table 5 summarizes the sample sizes for the reserve sample. This sample could be used if the initial sample is determined to have a larger than expected number of nontarget population lakes, resulting in an insufficient sample size. Alternatively, if additional funding is received to allow a larger sample size, the reserve sample of lakes could be used. Decisions regarding use of the reserve sample of lakes (or subsets of the reserve sample) will be made only by the USEPA Project Manager. Table 5. Number of Lakes (by Size Category and Year) Selected as a Reserve Sample. Lake area (ha) >l-5 >5-10 >10-50 >50-500 >500-5000 >5000 Total 1999 47 45 36 36 38 23 225 2000 48 52 39 26 29 31 225 2001 48 40 42 40 30 25 225 2002 49 42 41 22 37 34 225 All Years 192 179 158 124 134 113 900 Expected Weight 938.84 261.61 256.51 85.06 11.36 2.21 ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 21 of 36 8.0 SAMPLING METHODS 8.1 Target Species Field sampling procedures will follow the recommendations of USEPA's Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Volume I: Fish Sampling and Analysis, Second Edition (USEPA 1995) [or subsequent updates]. According to the guidance, the primary criteria for selecting target fishes is that the species: are commonly consumed in the study area, may potentially accumulate high concentrations of chemicals, and have a wide geographic distribution. Secondarily, the target species should be: easy to identify, abundant, easy to capture, and large enough to provide adequate tissue for analysis (i.e., harvestable size adult specimens that as a five-fish composite will provide at least 560 grams of edible tissue for analysis). Two distinct ecological groups offish, bottom-dwellers and predators, will be included as target fishes for this study. This permits monitoring of a wide variety of habitats, feeding strategies, and physiological factors that might result in differences in bioaccumulation of contaminants. Suggested target species are listed in Table 6 in order of preference (adapted from USEPA 1995). Additional target species may be added to the list of preferred targets on an as-needed basis, following discussion with the USEPA Project Manager and/or the Tetra Tech Task Leader. For example, additional Salmonid species (such as cutthroat trout or kokanee salmon) and Catostomid species (such as longnose sucker, largescale sucker, or bridgelip sucker) may need to be added to the target species for lakes in the northwestern United States. State personnel, with their knowledge of site-specific fisheries and human consumption patterns, will aid in the determination of the availability of target fishes. The criteria listed above must be considered when selecting target species other than those listed in Table 6. Every effort will be made to collect the desired species and number (Section 8.2) offish; however, the outcome of field sampling efforts will ultimately depend on the natural diversity and abundance offish in the study lakes. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 22 of 36 8.2 Composite Sampling The National Fish Tissue Study will involve composite sampling of predator/gamefish species and bottom-dwelling species (to be prepared as fillet composites and whole-body composites, respectively, by the sample preparation laboratory). Composite samples are cost-effective for estimating average tissue concentrations of target analytes in target species populations, and compositing ensures adequate sample mass for analysis of all target analytes. Table 6. Recommended Target Species for Inland Freshwaters (in Order of Preference). 11 If I -2- 1 2 E 3 Ł o 2Ł Family name Centrarchidae Percidae Percichthyidae Esocidae Salmonidae Cyprinidae Ictaluridae Catostomidae Common name Largemouth bass Smallmouth bass Black crappie White crappie Walleye Yellow perch White bass Northern pike Lake trout Brown trout Rainbow trout Brook trout Common carp Channel catfish Blue catfish Brown bullhead Yellow bullhead White sucker Scientific name Micropterus salmoides Micropterus dolomieu Pomoxis nigromaculatus Pomoxis annularis Stizostedion vitreum Percaflavescens Morone chrysops Esox lucius Salvelinus namaycush Salmo trutta Oncorhynchus mykiss Salvelinus fontinalis Cyprinus carpio Ictalurus punctatus Ictalurus furcatus Ameiurus nebulosus Ameiurus natalis Catostomus commersoni One predator/gamefish composite and one bottom-dwelling species composite will be collected from each target lake (Note: The USEPA Project Manager and/or the Tetra Tech Task Leader need to be notified if one of the ecological groups offish are not present or available from a target lake). Each composite will consist of five fish of adequate size (i.e., adult specimens that collectively will provide at least 560 grams of edible tissue for predators, and 560 grams of total body tissue for bottom-dwellers) to allow analysis of the target analytes. Fish retained for a composite sample must meet the following criteria: all be of the same species, satisfy any legal requirements of harvestable size (or weight), or at least be of consumable size if no legal harvest requirements are in effect, ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 23 of 36 be of similar size so that the smallest individual in a composite is no less than 75% of the total length of the largest individual, and be collected at the same time, i.e., collected as close to the same time as possible, but no more than one week apart (Note: Individual fish may have to be frozen until all fish to be included in the composite are available for delivery to the sample preparation laboratory). Accurate taxonomic identification is essential in assuring and defining the organisms that have been composited and submitted for analysis. Under no circumstances should individuals from different species be used in a single composite sample. Ideally, the target species composite should focus on the larger individuals commonly harvested by the local population. 8.3 Sample Collection Fish collection methods can be divided into two major categories, active and passive. Each has advantages and disadvantages. Active collection methods employ a wide variety of sampling devices including electrofishing units, seines, trawls, and angling equipment (hook and line). Although active collection requires greater fishing effort, it is usually more efficient than passive collection for covering a large number of sites and catching the relatively small number of individuals needed from each site for tissue analysis. The active collection methods generally require more field personnel and more expensive equipment than passive collection methods. Passive collection methods employ a wide array of sampling devices, including gill nets, fyke nets, trammel nets, hoop nets, pound nets, and d-traps. Passive collection methods generally require less fishing effort than active methods, but normally yield a much greater catch than would be required for a contaminant monitoring program. They are also time consuming to deploy. Passive collection devices (e.g., gill nets) must be checked frequently (e.g., at least once every 24 hours) to ensure a limited time lag between fish entrapment and sample preparation/preservation. Sampling Teams dedicated to the National Fish Tissue Study will be equipped with an array of both active and passive gears to ensure the collection of the desired target numbers and species offish. Selection of the most appropriate gear type(s) for a particular target lake will be at the discretion of the experienced on-site fisheries biologist. USEPA Regional/State/Tribal Sampling Teams and Contractor- affiliated Sampling Teams will be responsible for providing fisheries sampling gear and sampling vessels. The sample control center will provide sample packaging and shipping supplies. A list of equipment and expendable supplies is provided in Table 7. Sample collection, packaging, and shipment methods are presented as Appendix B, Standard Operating Procedure. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 24 of 36 Table 7. Equipment and Supply List for Fish Tissue Sampling. 1. Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment}"' 2. Electrofishing equipment - OPTIONAL (including variable voltage pulsator unit, generator, electrodes, wiring cables, dip nets, protective gloves, protective boots, and all necessary safety equipment}"' 3. Nets - OPTIONAL (including trawls, seines, gill nets, fyke nets, trammel nets, hoop nets, pound nets, trap nets)1"1 4. Angling equipment - OPTIONAL (including fishing rods, reels, line, terminal tackle, trot lines)1"' 5. Coast Guard-approved personal floatation devices 6. Maps of target lakes and access routes 7. Global Positioning System (GPS) unit - OPTIONAL <"> pH meter (including associated calibration supplies)("' 9. Livewell and/or buckets 10. Measuring board (millimeter scale) 11. Ice chests"' 12. Aluminum foil (solvent-rinsed and baked)* 13. Heavy-duty food grade polyethylene tubing*1 14. Large plastic (composite) bags( 15. Knife or scissors 16. Clean nitrile gloves * 17. Field Record Forms*' 18. Sample Identification Labels *' 19. Chain-of-Custody Forms *> 20. Chain-of-Custody Labels* 21. Scientific collection permit 22. Dry ice* 23. Black ballpoint pens and/or waterproof markers 24. Clipboard 25. Packing/strapping tape 26. Overnight courier airbills *' 27. Plastic cable ties*' 28. Plastic bubble-wrap*' 29. First aid kit and emergency telephone numbers ^ Selection and exact specifications at the discretion of the experienced on-site fisheries biologist. (b) Provided by the sample control center. As soon as fish are obtained via active collection methods, or removed from passive collection devices, they should be identified to species. Species identification should be conducted only by experienced personnel knowledgeable of the taxonomy of species in the waterbodies included in the fish contaminant monitoring program. Nontarget species, collected by the field team should be returned to the water. Individuals of the selected target species will be rinsed in ambient water to remove any ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 25 of 36 foreign material from the external surface, should be handled using clean nitrile gloves (provided by the sample control center), and placed in clean holding containers (livewell, buckets, etc.) to prevent contamination. Each fish of the selected target species should be measured to determine total body length (mm). Maximum body length should be measured, i.e., the length from the anterior-most part of the fish to the tip of the longest caudal finray (when the lobes of the caudal fin are depressed dorsoventrally). When sufficient numbers of the target species have been identified to make up a suitable composite sample (i.e., five individuals meeting the size criteria presented in Section 8.2), the species name, specimen lengths, and all other site and sampling information should be recorded on the Field Record Form (Appendix B). The field objective is for sampling teams to obtain a representative composite sample for both a predator and a bottom-dwelling species from each lake or reservoir selected for the National Fish Tissue Study. Each composite must consist of all the same species, individual fish must be of similar size (i.e., all within 75% of the length of the largest fish), and the composite must be able to deliver 560 grams offish tissue (fillets for predators, and whole bodies for bottom-dwellers) for chemical analysis. To obtain a representative sample of the targeted species in lakes and reservoirs (and particularly in large waterbodies), field teams should consider factors such as habitat and presence of contaminant gradients in planning sampling locations for the target lake. Ideally, the habitats suitable for target species would be determined for the lake, and up to three locations of that habitat would be randomly selected for sampling in the lake. If a contamination gradient may be present in the waterbody, then three locations across the gradient should be selected for sampling. For example, in reservoirs, the three locations may be in habitat near the inflow, middle, and outflow of the reservoir. The composite is intended to estimate the mean fish tissue contaminant concentration for the lake or reservoir. Given the diversity of lakes and reservoirs in the study, and given the multiple species that must be used, the study must rely on the local knowledge of the field teams in the selection of the representative composite sample. 9.0 SAMPLE HANDLING AND CUSTODY REQUIREMENTS 9.1 Sample Handling Clean nitrile gloves (provided by the sample control center) should be worn during the entire sample handling process, beginning with removing the fish from the sampling gear. Individuals of the selected target species should be rinsed in ambient water to remove any foreign material from the external surface. After initial processing to determine species and size, each of the five fish found to be suitable for the composite sample will be individually wrapped in extra heavy-duty aluminum foil (provided by the sample control center as solvent-rinsed, oven-baked sheets). For specimens with sharp fins, spines may be broken (via gloved hands or with the use of a tool covered with the aluminum foil provided by the sample control center) to prevent perforation of the wrapping materials. The broken section of the fins should be included with the fish sample. A Sample Identification Label (Appendix B) will be prepared for each aluminum foil-wrapped specimen. Each foil-wrapped fish will be placed into a waterproof plastic tubing that will be cut to size to fit the specimen (i.e., heavy duty food grade polyethylene tubing provided by the sample control center), and each end of the tubing will be sealed ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 26 of 36 with a plastic cable tie. The completed Sample Identification Label will be affixed to the cable tie, and the entire specimen package will be "double-bagged" (i.e., placed inside a large plastic bag with all the specimens of the same species from that site and sealed with another cable tie). Once packaged, samples should be immediately placed on dry ice for shipment. If samples will be carried back to a laboratory or other facility to be frozen before shipment, wet ice can be used to transport wrapped and bagged fish samples in the coolers to that laboratory or facility. If possible, all of the specimens in a composite sample should be kept together in the same shipping container (ice chest) for transport. Sampling Teams have the option, depending on site logistics, of: shipping the samples packed on dry ice (in sufficient quantities to keep samples frozen for up to 48 hours), via priority overnight delivery service (i.e., Federal Express), so that they arrive at the sample preparation laboratory within less than 24 hours from the time of sample collection, or freezing the samples within 24 hours of collection (at <-20°C), and storing the frozen samples until shipment within 1 week of sample collection (frozen samples will subsequently be packed on dry ice and shipped to the sample preparation laboratory via priority overnight delivery service to arrive within less than 24 hours from time of shipment). The time of sample collection, relinquishment by the sample team, and time of their arrival at the sample preparation laboratory must be recorded on the Chain-of-Custody Form (Appendix B). Field Sampling Teams should avoid shipping samples for weekend delivery to the sample preparation laboratory unless prior plans for such a delivery have been agreed upon with the sample control center. 9.2 Sample Integrity A critical requirement of the National Fish Tissue Study is the maintenance of sample integrity from the time of collection to the shipment and arrival at the final destination. Sample integrity is maintained by preventing the loss of contaminants that might be present in the sample and by taking precautions to avoid possible introduction of contaminants during handling. The loss of contaminants can be prevented in the field by ensuring that the sample collected remains intact, i.e., sample collection procedures should be performed with the intention of minimizing the laceration offish skin. Once a sample is collected, sample integrity is maintained through careful and controlled sample handling, storage, and preservation procedures (Section 9.1). Preventable sources of extraneous contamination can include the sampling gear, oils and greases on boats, spilled fuel, skin contact, contact with soil or sand, boat motor exhaust, and other potential sources. All potential sources should be identified before the onset and during sample collection, and ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 27 of 36 appropriate measures should be taken to minimize or eliminate them. Examples of preventative measures include the following: Collection nets should be free of any potential contaminants. The use of tarred collection nets is prohibited. Boats should be positioned so that engine exhaust does not fall on the deck area where samples are being handled. Ice chests and other sample storage containers should be scrubbed clean with detergent and rinsed with distilled water prior to use (containers originating from the sample control center will be prewashed and rinsed). Samples should not be placed directly on dry ice, but should be stored inside foil, plastic tubing (i.e., heavy-duty food grade polyethylene tubing as per Section 9.1), and plastic garbage bags first. Proper gloves (clean nitrile gloves) should be used when handling samples. 9.3 Custody Requirements As soon as possible following collection, the Sampling Team will begin the process of identifying, labeling, packaging, and storing the sample(s). Each sample will be identified and tracked with a unique numbering scheme as described in Section 6.0. This ten-character composite code followed by a two- digit specimen number will identify each sample on all documentation and records including the following: Field Record Form, Sample Identification Label, and Chain-of-Custody Form. Each sample (i.e., individual fish) will be labeled by affixing a Sample Identification Label (Appendix B) as per the instructions in Section 9.1. All sample label entries will be made with black indelible ink. The sample label will accompany each sample throughout the chain-of-custody. Each sample label will include the following information: project name (USEPA National Fish Tissue Study), site identification (lake name), ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 28 of 36 sample number (01 through 05), composite code (ten-digit code as in Section 6.0), date of sample (month/day/year), time of collection (military time), preservative used (dry ice or frozen), and collector's name (field team leader). Detailed documentation of the samples collected in the field (for shipment to the sample preparation laboratory) and information about the collection location will be recorded on a Field Record Form (Appendix B). One form must be completed for each sample composite. One page of the four-page carbonless copy form (Section 6.0) will be retained by the sampler, and the other copies will be included with sample shipment to the sample preparation laboratory. (The sample preparation laboratory will retain one copy, and be responsible for forwarding one copy to the sample control center and one copy to the Tetra Tech Task Leader.) All entries will be made in black ink and no erasures will be made. Each form will have the proper entry requirements, which includes the following information: composite code (ten digits as per Section 6.0), sampling date (month/day/year), time of collection (military time), collection method (e.g., gill net), collector's name (printed and signed), collector's affiliation, address, and telephone number, site name (lake name), site description (location of lake and area of lake sampled), lake type (e.g., natural lake), estimated maximum depth (meters), ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 29 of 36 fish species (common name), length (mm) of each specimen, location, date and time of collection for each specimen, and a simple sketch of the sampling site and sample collection points. All samples and composites will be transferred to the receiving laboratory (i.e., sample preparation laboratory) under chain of custody. The Chain-of-Custody Form (Appendix B) acts as a record of sample shipment and a catalog of the contents of each shipment (coinciding with information on the field record). The forms will be produced as five-page carbonless copies with one copy retained by the sampler and four for shipment to the laboratory (i.e., one for the sample preparation laboratory, one for the sample control center, one for the Tetra Tech Task Leader, and one for duplication and distribution to the analytical laboratories). The latter four copies will be placed in a waterproof plastic bag (provided by the sample control center) and sealed inside the shipping container. All Chain-of-Custody Form entries will be made in black ink and will include: the USEPA Proj ect Manager's name, address and telephone number (refer to the QAPP cover page), sampler's name and telephone number, project name (USEPA National Fish Tissue Study), page number (e.g., 1 of 1), sample location (lake name), collection date and time, composite code (ten-digit) and sample number (two-digit), preservative (dry ice or frozen), number of containers, type of analysis required (USEPA 274 PBT target analytes [including breakdown products and PCB congeners]), sampler's signature, sample date, and time, sampler relinquishment date and time, ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 30 of 36 laboratory recipient signature, and laboratory receipt date and time. Immediately following the packing of each shipping container (Section 9.1), each container (ice chest) will be secured with packaging tape and sealed with a Chain-of-Custody Label (provided by the laboratory). The Chain-of-Custody Label must contain the signature of the sampler and the date and time written in ink. The seal must be affixed such that the shipping container cannot be opened without breaking the seal (e.g., label adhered across the ice chest latch), so as to protect and document the integrity of the contents from field to laboratory. 10.0 ANALYTICAL METHODS REQUIREMENTS Samples will be shipped (Section 9.1) under chain of custody to locations designated by the USEPA Project Manager for processing and analytical testing. Sample processing and analytical testing and methods are outside the scope of this QAPP and therefore are not addressed herein, but will be discussed in the Analytical Activities QAPP. 11.0 QUALITY CONTROL REQUIREMENTS Data quality is addressed, in part, by consistent performance of valid procedures documented in the standard operating procedures (Appendix B). It is enhanced by the training and experience of project staff (Section 5.0) and documentation of project activities (Section 6.0). This QAPP, a field sampling plan, and training materials will be distributed to all USEPA/Regional/State/ Tribal Fish Sampling Coordinators, and, in turn, to sampling personnel. Orientation sessions will be set up by EPA Regions to distribute and discuss project materials (Section 5.0). USEPA Regional/State/Tribal Fish Sampling Coordinators and Field Team Leaders will be required to view the training materials, read the QAPP, and verify in writing that they read or viewed the materials and understood the procedures and requirements. 12.0 INSTRUMENT/EQUIPMENT TESTING, INSPECTION, AND MAINTENANCE REQUIREMENTS All field equipment will be inspected prior to sampling activities to ensure that proper use requirements are met (e.g., boats or electrofishers are operating correctly, nets are without defects, pH meter properly calibrated). Inspection of field equipment will occur well in advance of the field operation to allow time for replacement or repair of defective equipment, and the field team will be equipped with proper backup equipment to prevent lost time on site. One member of each field team should gather and inspect all equipment on the equipment and supply list (Table 7) prior to each sampling event. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 31 of 36 13.0 INSTRUMENT CALIBRATION AND FREQUENCY All pH meters used by field teams will be calibrated according to the manufacturer's operating instructions, on a daily basis, while in use (Appendix C). 14.0 INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPLIES AND CONSUMABLES Careful and thorough planning is necessary to ensure the efficient and effective completion of the field sample collection task. A general checklist of field equipment and supplies is provided in Table 7. Sampling gear will be provided by each field team, and most sample preparation and shipment supplies will be provided by the sample control center. It will be the responsibility of each field team to gather and inspect the necessary sampling gear prior to the sampling event and to inspect the sample packaging and shipping supplies received from the laboratory. Defective packaging and shipping supplies (e.g., torn or damaged polyethylene sample tubing) will be discarded, and, if necessary, the field team will contact the sample control center to obtain replacement supplies. 15.0 DATA ACQUISITION REQUIREMENTS (NONDIRECT MEASUREMENTS) Nondirect measurements will include identification and/or verification of each sample lake location (i.e., latitude and longitude). Coordinates of the target lakes are provided in Appendix A as decimal degrees and conventional degrees, minutes, and seconds. USEPA Regional/State/ Tribal Fish Sampling Coordinators or Field Sampling Teams having corrections to the Appendix A coordinates for a particular target lake (based on USGS quadrangle, or equivalent, map verification), need to report those corrections to the USEPA Project Manager (telephone 202/260-7055) or Tetra Tech Task Leader (telephone 410/356-8993). 16.0 DATA MANAGEMENT Samples will be documented and tracked via Sample Identification Labels, Field Record Forms, and Chain-of-Custody Forms (Section 6.0). Since the sampling effort is a cooperative one involving many different partner agencies and groups, the diligence of the Field Sampling Teams in completion of the proper records is essential. Field team leaders will be responsible for reviewing all completed field forms. Any corrections should be noted, initialed, and dated by the reviewer (Section 6.0). As mentioned in Section 6.0, Field Record Forms and Chain-of-Custody Forms will each be prepared and replicated in the field, via multiple page "carbonless copy" forms. The sampler will retain one copy each of the Field Record and Chain-of-Custody Forms, and the remaining copies will be delivered to the sample preparation laboratory with the samples. Shipment of samples to the sample preparation laboratory (Section 9.1) must be conducted by a delivery service that provides constant tracking of shipments (e.g., Federal Express). Laboratory sample log-in and data management procedures are beyond the scope of this QAPP. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 32 of 36 The sample preparation laboratory will retain one copy of each Field Record Form and Chain-of- Custody Form, and will forward a copy of each to the Tetra Tech Task Leader. All form copies obtained by Tetra Tech will be maintained in a project file during the active phase of the project, and for a period of 2 years following completion of the project (unless otherwise directed by USEPA). Upon completion of annual sampling activities, Tetra Tech will develop a field collection effort summary (i.e., a detailed listing of all sampling participants, sampling locations, and specimens collected) based on information recorded by all Sampling Teams on the Field Record Forms. The Field Record Form data will be entered into an ExcelŽ spreadsheet to create the annual summary. All data entries will be checked for errors in transcription and computer input by a minimum of two persons. If there is any indication that requirements for sample integrity or data quality have not been met, the Tetra Tech QA Officer will be notified immediately (with an accompanying explanation of the problems encountered). All computer files associated with the project will be stored in a project subdirectory by Tetra Tech, and will be copied to disk for archive for the two years subsequent to project completion (unless otherwise directed by the USEPA Project Manager). C. ASSESSMENT/OVERSIGHT 17.0 ASSESSMENT AND RESPONSE ACTIONS Assessment activities and corrective response actions have been identified to ensure that sample collection activities are conducted as prescribed and that the measurement quality objectives and data quality objectives established by USEPA are met. The QA program under which this project will operate includes performance and system audits with independent checks of the data obtained from sampling activities. Either type of audit could indicate the need for corrective action. The essential steps in the program are as follows: identify and define the problem, assign responsibility for investigating the problem, investigate and determine the cause of the problem, assign and accept responsibility for implementing appropriate corrective action, establish effectiveness of and implement the corrective action, and verify that the corrective action has eliminated the problem. Immediate corrective actions form part of normal operating procedures and are noted on project Field Record Forms. Problems not solved this way require more formalized, long-term ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 33 of 36 corrective action. In the event that quality problems requiring attention are identified, the Tetra Tech Task Leader and/or Tetra Tech QA Officer will determine whether attainment of acceptable data quality requires either short- or long-term actions. Failure in an analytical system (e.g., performance requirements are not met) and corrective actions for those failures are beyond the scope of this QAPP. Communication and oversight will proceed from Field Sampling Team Leaders (e.g., senior fisheries biologist) to the Tetra Tech Task Leader and the USEPA Regional/State/Tribal Fish Sampling Coordinators. The Tetra Tech Task Leader will be on-call throughout the entire sampling period (Section 7.2) to address questions and receive communications of sampling status from the Field Sampling Teams. The Tetra Tech Task Leader will communicate the status of the sampling activities to the USEPA Project Manager on a weekly basis (at a minimum). The USEPA Regional/State/Tribal Fish Sampling Coordinators and Tetra Tech Task Leader will immediately consult with the Tetra Tech QA Officer and USEPA Project Manager regarding any difficulties encountered during sample collection activities. The Tetra Tech QA Officer will initiate the corrective action system described above, documenting the nature of the problem and ensuring that the recommended corrective action is carried out. The USEPA Project Manager and/or the Tetra Tech QA Officer will work with the USEPA Regional/ State/Tribal Fish Sampling Coordinators and Tetra Tech Task Leader to determine the best way to rectify the problem and obtain accurate and useable data. When corrective actions have been taken and a sufficient time period has elapsed that allows a response, the response will be compared with project goals by the USEPA Project Manager. The Tetra Tech QA Officer will verify that the corrective action has been appropriately addressed to eliminate the problem. The USEPA QA Manager has the authority to stop work on the project if problems affecting data quality are identified that will require extensive effort to resolve. The USEPA Project Manager will consult with the USEPA QA Manager regarding any and all corrective actions and stop work orders. Performance audits are qualitative checks on different segments of project activities, and are most appropriate for sampling, analysis, and data processing activities. Field audits will be conducted periodically in accordance with Agency requirements and availability of resources. Performance audit techniques include checks on sampling equipment, measurements, and the analysis of data quality using QC and spiked samples. Analytical performance audits are beyond the scope of this QAPP. The USEPA Regional/State/Tribal Fish Sampling Coordinators and/or the Tetra Tech Task Leader will be responsible for overseeing work as it is performed, and periodically conducting QC checks during the sample collection phase of this project. System audits are qualitative reviews of project activity to check that the overall quality program is functioning and that the appropriate QC measures identified in the QAPP are being implemented. The Tetra Tech QA Officer will conduct one internal system audit during the project and report the results to the USEPA Project Manager on Tetra Tech's standard Audit Report Form. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 34 of 36 18.0 REPORTS TO MANAGEMENT Following completion of the system audit, the Tetra Tech QA Officer will prepare an Audit Report Form and submit copies to both the USEPA Project Manager and the USEPA QA Officer. Upon completion of weekly sampling activities, the Tetra Tech Task Leader will contact the USEPA Project Manager to summarize Field Sampling Team progress for the preceding week and submit a weekly progress report detailing the sampling activities. Following completion of annual field sampling activities, Tetra Tech will prepare an annual field collection effort summary (i.e., detailed listing of all sampling participants, sampling locations, and specimens collected) for review by the USEPA Project Manager. Following incorporation of USEPA Project Manager comments and final approval, the summary will be used to report back to USEPA Regional/State/Tribal participants to document collective sampling progress for each study year. D. DATA VALIDATION AND USABILITY 19.0 DATA REVIEW, VALIDATION, AND VERIFICATION REQUIREMENTS Data validation and review services provide a method for determining the usability and limitations of data, and provide a standardized data quality assessment. All Field Record Forms and Chain-of- Custody records will be reviewed by the Tetra Tech Task Leader (assisted by the QA Officer, as needed) for completeness and correctness. Tetra Tech will be responsible for reviewing data entries and transmittals for completeness and adherence to QA requirements. Data quality will be assessed by comparing entered data to original data or by comparing results with the measurement performance criteria summarized in Section 4.2 to determine whether to accept, reject, or qualify the data. Results of the review and validation processes will be reported to the USEPA Project Manager. 20.0 VALIDATION AND VERIFICATION METHODS All Field Record Forms and Chain-of-Custody records will be reviewed by the Tetra Tech Task Leader. The Tetra Tech QA Officer will review a minimum of five percent of the Field Record Forms and Chain-of-Custody records. Any discrepancies in the records will be reconciled with the appropriate associated field personnel and will be reported to the USEPA Project Manager. Analytical validation and verification methods are outside of the scope of this QAPP. The submission of samples to the sample preparation laboratory will include Field Record Forms and Chain-of- Custody Forms documenting sampling time and date. This information will be checked by the receiving laboratory to ensure that holding times (Section 9.1) have not been exceeded. Violations of holding times will be reported (by the laboratory) to the USEPA Project Manager and the Tetra Tech Task Leader via the USEPA Sample Analysis Manager, and the USEPA Project Manager will discuss with the USEPA Sample Analysis Manager whether or not to issue a stop work order for analysis of that particular sample. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 35 of 36 21.0 RECONCILIATION WITH DATA QUALITY OBJECTIVES As soon as possible following completion of the sample collection task, precision, accuracy, and completeness measures will be assessed by Tetra Tech and compared with the criteria discussed in Section 4.0. This will represent the final determination of whether the data collected are of the correct type, quantity, and quality to support their intended use for this project. Any problems encountered in meeting the performance criteria (or uncertainties and limitations in the use of the data) will be discussed with the USEPA Project Manager, and will be reconciled, if possible. ------- National Study of Chemical Residues in Lake Fish Tissue QAPP Final Date: 22 May 2000 Page 36 of 36 LITERATURE CITED NOAA. 1993. Sampling and Analytical Methods of the National Status and Trends Program National Benthic Surveillance and Mussel Watch Projects 1984-1992, Volumes I-IV. National Oceanic and Atmospheric Administration, Coastal Monitoring and Bioeffects Assessment Division, Office of Ocean Resources Conservation and Assessment, National Ocean Service, Silver Spring, MD. NOAA Technical Memorandum NOS ORCA 71. Reference No. 024. Olsen, A.R., D.L. Stevens, Jr., and D. White. 1998. Application of global grids in environmental sampling. Computing Science and Statistics. 30:279-284. U.S. Environmental Protection Agency (USEPA). 1992. National Study of Chemical Residues in Fish. U.S. Environmental Protection Agency, Office of Science and Technology, Washington, D.C. EPA 823-R-92-008. U.S. Environmental Protection Agency (USEPA). 1995. Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Volume 1: Fish Sampling and Analysis, Second Edition. U.S. Environmental Protection Agency, Office of Water, Washington, D.C. EPA 823-R-95-007. U.S. Environmental Protection Agency (USEPA). 1999a. EPA Requirements for Quality Assurance Project Plans. U.S. Environmental Protection Agency, Quality Assurance Division, Washington, D.C. Interim Final. EPA/QA/R-5. U.S. Environmental Protection Agency (USEPA). 1999b. National Study of Chemical Residues in Lake Fish Tissue: Study Design. U.S. Environmental Protection Agency, Office of Science and Technology, Washington, D.C. U.S. Environmental Protection Agency (USEPA). 1999c. Update: Listing of Fish and Wildlife Advisories. Fact Sheet. U.S. Environmental Protection Agency, Office of Water, Washington, D.C. EPA 823-F-99-005. ------- Appendix A Randomly Selected List of Target Lakes ------- ------- OWOW99 Site-ID OWOW99-0022 OWOW99-0072 OWOW99-0136 OWOW99-0147 OWOW99-0161 OWOW99-0197 OWOW99-0486 OWOW99-0511 OWOW99-0547 OWOW99-0560 OWOW99-0622 OWOW99-0647 OWOW99-0923 OWOW99-0947 OWOW99-0961 OWOW99-1072 OWOW99-1436 OWOW99-1472 OWOW99-1497 OWOW99-0047 OWOW99-0097 OWOW99-0122 OWOW99-0143 OWOW99-0171 OWOW99-0222 OWOW99-0497 Long. DD -87.3315 -85.0969 -87.1320 -86.0285 -87.0398 -87.3823 -86.3385 -85.5705 -87.1202 -85.1396 -85.3245 -85.7285 -87.2961 -87.4428 -86.2978 -86.7524 -85.9938 -86.0533 -86.3959 -93.9120 -91.9745 -91.3425 -92.2420 -91.7495 -90.3498 -93.8325 Lat. DD 33.9487 31.9344 34.0809 31.1530 34.6639 32.0983 33.3199 34.0993 33.3243 32.4487 31.1539 32.4403 31.4475 32.8863 34.1229 32.3890 33.6132 32.3590 33.1694 35.2279 34.9647 35.7756 36.4063 34.1479 34.8895 35.5319 Lake Name WALTER F GEORGE RES Lewis Smith Lake Wheeler Lake Clark's lake Pine Lake Kelley Lake Payne Lake Jones Bluff Lake Candles Lake NORFOLK L St. AL AL AL AL AL AL AL AL AL AL AL AL AL AL AL AL AL AL AL AR AR AR AR AR AR AR County WALKER BARBOUR WINSTON GENEVA WILCOX TALLADEGA CHEROKEE JEFFERSON RUSSELL HOUSTON MACON MONROE HALE MARSHALL LOWNDES CALHOUN MONTGOMERY TALLADEGA LOGAN LONOKE INDEPENDENCE BAXTER JEFFERSON CRITTENDEN FRANKLIN Lake Area (ha) 4 15282 8793 6 27143 4738 16 48 7 3 3 3 2 46 3 5063 7 1 26 6 3 2 7546 5 2 166 Yr. 1 1 1 1 1 1 2 2 2 2 2 2 3 3 3 3 4 4 4 1 1 1 1 1 1 2 Longitude Deg 87 85 87 86 87 87 86 85 87 85 85 85 87 87 86 86 85 86 86 93 91 91 92 91 90 93 Min 19 5 7 1 2 22 20 34 7 8 19 43 17 26 17 45 59 3 23 54 58 20 14 44 20 49 Sec 53.40 48.84 55.20 42.60 23.21 56.28 18.60 13.80 12.72 22.56 28.20 42.60 45.96 34.08 52.08 8.64 37.68 11.88 45.24 43.20 28.20 33.00 31.20 58.20 59.28 57.00 Latitude Deg 33 31 34 31 34 32 33 34 33 32 31 32 31 32 34 32 33 32 33 35 34 35 36 34 34 35 Min 56 56 4 9 39 5 19 5 19 26 9 26 26 53 7 23 36 21 10 13 57 46 24 8 53 31 Sec 55.32 3.84 51.24 10.80 49.93 53.88 11.64 57.48 27.48 55.32 14.04 25.08 51.00 10.68 22.44 20.40 47.52 32.40 9.84 40.44 52.92 32.16 22.68 52.44 22.20 54.84 1 of 35 ------- OWOW99 Site-ID OWOW99-0523 OWOW99-0571 OWOW99-0623 OWOW99-0922 OWOW99-0971 OWOW99-1022 OWOW99-1046 OWOW99-1371 OWOW99-1396 OWOW99-1398 OWOW99-1447 OWOW99-1449 OWOW99-1493 OWOW99-1522 OWOW99-0045 OWOW99-0569 OWOW99-0595 OWOW99-1020 OWOW99-1044 OWOW99-1520 OWOW99-0001 OWOW99-0002 OWOW99-0018 OWOW99-0026 OWOW99-0027 OWOW99-0051 Long. DD -93.7021 -92.1631 -93.1037 -91.7543 -91.0572 -90.7207 -92.5217 -93.3895 -91.3933 -94.0040 -91.6630 -93.2374 -93.9496 -90.3370 -111.2923 -109.5274 -110.0558 -114.6362 -109.4212 -114.3657 -120.4193 -119.2349 -114.5414 -123.7864 -119.7812 -122.1163 Lat. DD 33.2410 35.5610 34.1847 34.7737 34.2542 34.7570 34.5720 34.6169 34.4663 33.7506 34.9565 34.2571 36.3670 34.9306 33.5876 34.1152 34.3240 35.4539 33.9097 34.5009 41.9579 36.8747 33.5724 40.0640 38.1627 37.7861 Lake Name LOUCHITA Millwood Lake Degray Lake BEAVER RES Aoache Lake Carnero Lake White Lake L MOJAVE Crescent Lake L HAVASU Goose Lake St. AR AR AR AR AR AR AR AR AR AR AR AR AR AR AZ AZ AZ AZ AZ AZ CA CA CA CA CA CA County LAFAYETTE CLEBURNE CLARK LONOKE PHILLIPS LEE SALINE GARLAND ARKANSAS LITTLE RIVER PRAIRIE CLARK BENTON CRITTENDEN MARICOPA APACHE NAVAJO MOHAVE APACHE MOHAVE FRESNO RIVERSIDE HUMBOLDT TUOLUMNE ALAMEDA Lake Area (ha) 21 4803 152 7 2 1 4 15816 24 9668 6 4576 8311 872 888 27 10 10446 64 7223 0 2337 7 25 3 309 Yr. 2 2 2 3 3 3 3 4 4 4 4 4 4 4 1 2 2 3 3 4 1 1 1 1 1 1 Longitude Deg 93 92 93 91 91 90 92 93 91 94 91 93 93 90 111 109 110 114 109 114 120 119 114 123 119 122 Min 42 9 6 45 3 43 31 23 23 0 39 14 56 20 17 31 3 38 25 21 25 14 32 47 46 6 Sec 7.56 47.16 13.32 15.48 25.92 14.52 18.12 22.20 35.88 14.40 46.80 14.64 58.56 13.20 32.28 38.64 20.88 10.32 16.32 56.52 9.59 5.64 29.04 11.04 52.32 58.68 Latitude Deg 33 35 34 34 34 34 34 34 34 33 34 34 36 34 33 34 34 35 33 34 41 36 33 40 38 37 Min 14 33 11 46 15 45 34 37 27 45 57 15 22 55 35 6 19 27 54 30 57 52 34 3 9 47 Sec 27.60 39.60 4.92 25.32 15.12 25.20 19.20 0.84 58.68 2.16 23.40 25.56 1.20 50.16 15.36 54.72 26.40 14.04 34.92 3.24 28.58 28.92 20.64 50.40 45.72 9.96 2 of 35 ------- OWOW99 Site-ID OWOW99-0077 OWOW99-0118 OWOW99-0126 OWOW99-0128 OWOW99-0151 OWOW99-0201 OWOW99-0452 OWOW99-0468 OWOW99-0476 OWOW99-0477 OWOW99-0503 OWOW99-0551 OWOW99-0577 OWOW99-0601 OWOW99-0603 OWOW99-0651 OWOW99-0953 OWOW99-0977 OWOW99-1002 OWOW99-1026 OWOW99-1076 OWOW99-1118 OWOW99-1351 OWOW99-1378 OWOW99-1402 OWOW99-1418 Long. DD -120.4489 -116.8157 -122.7705 -118.1459 -121.3599 -122.1881 -118.6846 -116.7810 -122.3975 -119.0280 -121.1275 -120.5188 -120.8588 -121.1105 -118.1823 -121.1289 -119.4818 -118.9776 -120.8496 -120.1551 -121.8182 -115.8314 -120.4928 -120.8460 -117.9516 -118.0490 Lat. DD 40.5446 34.0550 39.0266 35.0290 39.5799 41.4196 37.1583 32.9124 40.8253 38.0050 37.0439 40.2272 37.1225 38.4797 34.2971 41.5180 36.0562 37.3797 37.8529 38.9911 38.0663 33.3079 39.4114 35.3417 36.1876 33.6996 Lake Name Clear Lake Lake Oroville SHASTA L Mono Lake San Luis Res Salton Sea St. CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA CA County LASSEN SAN BERNARDINO LAKE KERN BUTTE SISKIYOU FRESNO SAN DIEGO SHASTA MONO MERCED PLUMAS MERCED SACRAMENTO LOS ANGELES MODOC TULARE FRESNO STANISLAUS EL DORADO SOLANO IMPERIAL NEVADA SAN LUIS OBISPO INYO ORANGE Lake Area (ha) 11 74 15956 1 1730 294 2 590 5468 16302 5214 2 6 2 33 55 44 755 719 8 476 94543 89 858 687 6 Yr. 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 Longitude Deg 120 116 122 118 121 122 118 116 122 119 121 120 120 121 118 121 119 118 120 120 121 115 120 120 117 118 Min 26 48 46 8 21 11 41 46 23 1 7 31 51 6 10 7 28 58 50 9 49 49 29 50 57 2 Sec 56.04 56.52 13.80 45.24 35.64 17.16 4.56 51.60 51.00 40.80 39.00 7.68 31.68 37.80 56.28 44.04 54.48 39.36 58.56 18.36 5.52 53.04 34.08 45.60 5.76 56.40 Latitude Deg 40 34 39 35 39 41 37 32 40 38 37 40 37 38 34 41 36 37 37 38 38 33 39 35 36 33 Min 32 3 1 1 34 25 9 54 49 0 2 13 7 28 17 31 3 22 51 59 3 18 24 20 11 41 Sec 40.56 18.00 35.76 44.40 47.64 10.56 29.88 44.64 31.08 18.00 38.04 37.92 21.00 46.92 49.56 4.80 22.32 46.92 10.44 27.96 58.68 28.44 41.04 30.12 15.36 58.56 3 of 35 ------- OWOW99 Site-ID OWOW99-1426 OWOW99-1427 OWOW99-1502 OWOW99-1526 OWOW99-1528 OWOW99-1551 OWOW99-0019 OWOW99-0028 OWOW99-0078 OWOW99-0176 OWOW99-0469 OWOW99-0478 OWOW99-0552 OWOW99-0903 OWOW99-0969 OWOW99-0994 OWOW99-1003 OWOW99-1394 OWOW99-1428 OWOW99-1569 OWOW99-0117 OWOW99-0938 OWOW99-1117 OWOW99-1538 OWOW99-0025 OWOW99-0050 Long. DD -122.7696 -119.4148 -121.9981 -120.7485 -120.4040 -120.1479 -102.3823 -104.7065 -107.3337 -108.0405 -102.2763 -105.0226 -106.2063 -104.4631 -106.5172 -103.7030 -105.0564 -107.8886 -105.3511 -104.8543 -71.9430 -73.4959 -72.9549 -72.7665 -81.8469 -81.9264 Lat. DD 40.8951 37.6232 37.4396 38.7982 34.9692 41.7342 37.4862 40.0127 39.7020 39.0011 40.0854 40.5558 40.0177 40.8024 37.4731 38.1887 40.7888 37.8946 40.8820 39.6397 42.0115 41.3409 41.9704 41.5074 28.4754 28.6951 Lake Name Claire Engle Upper Alkali Lake (dry) Quinebaug River Rainbow Lake Barkhamsted Reservoir Foster Lake St. CA CA CA CA CA CA CO CO CO CO CO CO CO CO CO CO CO CO CO CO CT CT CT CT FL FL County TRINITY MADERA SANTA CLARA EL DORADO SANTA BARBARA MODOC BACA WELD GARFIELD DELTA YUMA LARIMER GRAND WELD RIO GRANDE CROWLEY LARIMER SAN MIGUEL LARIMER ARAPAHOE WINDHAM FAIRFIELD LITCHFIELD NEW HAVEN LAKE LAKE Lake Area (ha) 6757 4 61 9 941 11196 22 6 9 6 7 3 546 1 6 2239 10 2 108 347 11 15 891 4 78 10 Yr. 4 4 4 4 4 4 1 1 1 1 2 2 2 3 3 3 3 4 4 4 1 3 3 4 1 1 Longitude Deg 122 119 121 120 120 120 102 104 107 108 102 105 106 104 106 103 105 107 105 104 71 73 72 72 81 81 Min 46 24 59 44 24 8 22 42 20 2 16 1 12 27 31 42 3 53 21 51 56 29 57 45 50 55 Sec 10.56 53.28 53.16 54.60 14.40 52.44 56.28 23.40 1.32 25.80 34.68 21.36 22.68 47.16 1.92 10.80 23.04 18.96 3.96 15.48 34.80 45.24 17.64 59.40 48.84 35.04 Latitude Deg 40 37 37 38 34 41 37 40 39 39 40 40 40 40 37 38 40 37 40 39 42 41 41 41 28 28 Min 53 37 26 47 58 44 29 0 42 0 5 33 1 48 28 11 47 53 52 38 0 20 58 30 28 41 Sec 42.36 23.52 22.56 53.52 9.12 3.12 10.32 45.72 7.20 3.96 7.44 20.88 3.72 8.64 23.16 19.32 19.68 40.56 55.20 22.92 41.40 27.24 13.44 26.64 31.44 42.36 4 of 35 ------- OWOW99 Site-ID OWOW99-0060 OWOW99-0075 OWOW99-0100 OWOW99-0125 OWOW99-0135 OWOW99-0150 OWOW99-0160 OWOW99-0175 OWOW99-0200 OWOW99-0225 OWOW99-0475 OWOW99-0498 OWOW99-0500 OWOW99-0510 OWOW99-0525 OWOW99-0535 OWOW99-0550 OWOW99-0574 OWOW99-0600 OWOW99-0610 OWOW99-0625 OWOW99-0650 OWOW99-0675 OWOW99-0925 OWOW99-0960 OWOW99-0972 Long. DD -82.3394 -81.5695 -82.3507 -80.9404 -81.8437 -80.7960 -80.5882 -82.2463 -84.9271 -82.2637 -81.6036 -86.3279 -81.6221 -80.9802 -81.1501 -81.2028 -80.6610 -81.9198 -82.2659 -81.6416 -80.2610 -82.5276 -81.8420 -81.8724 -81.4955 -85.4548 Lat. DD 30.0367 24.7544 28.9242 27.9389 29.1804 27.1752 27.7803 27.5928 30.6812 28.3406 28.5265 30.4826 28.6191 28.2704 26.4247 29.5902 24.9913 29.0737 27.9661 29.4031 26.0262 29.4948 27.8500 28.9253 28.6981 30.7352 Lake Name Tsala Apopka Lake Lake Okeechobee Lake Apopka St. FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL County UNION MONROE OSCEOLA MARION OKEECHOBEE INDIAN RIVER MANATEE JACKSON PASCO ORANGE WALTON ORANGE OSCEOLA HENDRY FLAGLER MONROE MARION HILLSBOROUGH PUTNAM BROWARD ALACHUA POLK LAKE ORANGE WASHINGTON Lake Area (ha) 363 24 0 2 140 4830 3 2 3 62 76 2 12439 5 24 111 53 7 22 3 5 7 130 9 23 5 Yr. 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 Longitude Deg 82 81 82 80 81 80 80 82 84 82 81 86 81 80 81 81 80 81 82 81 80 82 81 81 81 85 Min 20 34 21 56 50 47 35 14 55 15 36 19 37 58 9 12 39 55 15 38 15 31 50 52 29 27 Sec 21.84 10.20 2.52 25.44 37.32 45.60 17.52 46.68 37.56 49.32 12.96 40.44 19.56 48.72 0.36 10.08 39.60 11.28 57.24 29.76 39.60 39.36 31.20 20.64 43.80 17.28 Latitude Deg 30 24 28 27 29 27 27 27 30 28 28 30 28 28 26 29 24 29 27 29 26 29 27 28 28 30 Min 2 45 55 56 10 10 46 35 40 20 31 28 37 16 25 35 59 4 57 24 1 29 51 55 41 44 Sec 12.12 15.84 27.23 20.04 49.44 30.72 49.08 34.08 52.32 26.16 35.40 57.36 8.76 13.44 28.92 24.72 28.68 25.32 57.96 11.16 34.32 41.28 0.00 31.08 53.16 6.72 5 of 35 ------- OWOW99 Site-ID OWOW99-0975 OWOW99-1000 OWOW99-1025 OWOW99-1050 OWOW99-1060 OWOW99-1075 OWOW99-1100 OWOW99-1125 OWOW99-1385 OWOW99-1400 OWOW99-1425 OWOW99-1450 OWOW99-1475 OWOW99-1485 OWOW99-1500 OWOW99-1525 OWOW99-1535 OWOW99-1550 OWOW99-1575 OWOW99-0011 OWOW99-0036 OWOW99-0061 OWOW99-0086 OWOW99-0111 OWOW99-0185 OWOW99-0186 Long. DD -81.4995 -81.3723 -82.6637 -82.3075 -81.8922 -85.7628 -82.3138 -81.0849 -81.4253 -81.8260 -81.4320 -81.7713 -83.5613 -82.1732 -80.9613 -81.9134 -80.8242 -81.8328 -81.7677 -82.0992 -84.6784 -83.8010 -85.1335 -83.2689 -82.4576 -82.7802 Lat. DD 27.7380 28.2325 28.2997 27.4795 29.6284 30.4994 26.8617 28.1410 28.9124 26.8045 28.1606 28.8391 30.0024 29.4535 25.3195 27.7037 28.9187 26.3086 27.9878 32.5078 31.9560 34.6489 33.0623 31.5345 31.0359 34.0842 Lake Name L TOHOPEKALIGA Orange Lake West Point Lake St. FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL FL GA GA GA GA GA GA GA County POLK OSCEOLA PASCO MANATEE PUTNAM WASHINGTON CHARLOTTE OSCEOLA LAKE CHARLOTTE OSCEOLA LAKE TAYLOR MONROE POLK VOLUSIA COLLIER POLK CANDLER STEWART WHITE TROUP IRWIN WARE ELBERT Lake Area (ha) 1400 7643 8 593 18 20 16 7 3 6 57 2 6 5142 50 113 4 13 259 8 1 16 9215 3 4 2 Yr. 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 1 1 1 1 1 1 1 Longitude Deg 81 81 82 82 81 85 82 81 81 81 81 81 83 82 80 81 80 81 81 82 84 83 85 83 82 82 Min 29 22 39 18 53 45 18 5 25 49 25 46 33 10 57 54 49 49 46 5 40 48 8 16 27 46 Sec 58.20 20.28 49.32 27.00 31.92 46.08 49.68 5.64 31.08 33.60 55.20 16.68 40.68 23.66 40.68 48.24 27.12 58.08 3.72 57.12 42.24 3.60 0.60 8.04 27.36 48.72 Latitude Deg 27 28 28 27 29 30 26 28 28 26 28 28 30 29 25 27 28 26 27 32 31 34 33 31 31 34 Min 44 13 17 28 37 29 51 8 54 48 9 50 0 27 19 42 55 18 59 30 57 38 3 32 2 5 Sec 16.80 57.00 58.92 46.20 42.24 57.84 42.12 27.60 44.64 16.20 38.16 20.76 8.64 12.46 10.20 13.32 7.32 30.96 16.08 28.08 21.60 56.04 44.28 4.20 9.24 3.12 6 of 35 ------- OWOW99 Site-ID OWOW99-0461 OWOW99-0586 OWOW99-0636 OWOW99-0661 OWOW99-0911 OWOW99-0950 OWOW99-0986 OWOW99-1011 OWOW99-1035 OWOW99-1085 OWOW99-1097 OWOW99-1 1 1 1 OWOW99-1360 OWOW99-1386 OWOW99-1411 OWOW99-1461 OWOW99-1511 OWOW99-1547 OWOW99-1561 OWOW99-0082 OWOW99-0165 OWOW99-0540 OWOW99-0615 OWOW99-0907 OWOW99-0965 OWOW99-1040 Long. DD -82.2751 -83.1705 -83.2274 -82.7082 -83.4105 -84.3761 -81.6357 -84.6197 -84.6319 -82.5690 -83.8326 -82.7243 -84.9227 -85.2008 -83.8231 -82.3983 -83.2477 -84.9136 -83.2858 -95.7467 -93.6948 -93.7902 -95.8102 -94.8276 -92.3738 -93.7316 Lat. DD 32.5185 31.6930 34.0646 32.8112 33.0359 30.6983 32.9502 32.3871 34.1368 31.0989 30.8728 31.2453 33.6541 34.2992 31.1853 33.6589 31.8803 30.7852 33.2307 43.3695 42.8389 40.8780 40.7771 43.4883 40.9740 41.7532 Lake Name J Strom Thurmond Res LSEMINOLE Lake Sinclair Morse Lake Unnamed lake Percival Lake Eagle Lake Sand pit Saylorville Lake St. GA GA GA GA GA GA GA GA GA GA GA GA GA GA GA GA GA GA GA IA IA IA IA IA IA IA County EMANUEL BEN HILL MADISON WASHINGTON JONES GRADY SCREVEN MARION CHEROKEE WARE THOMAS ATKINSON CARROLL FLOYD COLQUITT COLUMBIA WILCOX SEMINOLE PUTNAM OSCEOLA WRIGHT DECATUR FREMONT EMMET WAPELLO POLK Lake Area (ha) 6 3 33 13 1 13 2 10 4661 3 5 3 6 12 4 10307 40 5138 2071 6 41 3 6 82 13 2041 Yr. 2 2 2 2 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 1 1 2 2 3 3 3 Longitude Deg 82 83 83 82 83 84 81 84 84 82 83 82 84 85 83 82 83 84 83 95 93 93 95 94 92 93 Min 16 10 13 42 24 22 38 37 37 34 49 43 55 12 49 23 14 54 17 44 41 47 48 49 22 43 Sec 30.36 13.80 38.64 29.52 37.80 33.96 8.52 10.92 54.84 8.40 57.36 27.48 21.72 2.88 23.16 53.88 51.72 48.96 8.88 48.12 41.28 24.72 36.72 39.36 25.68 53.76 Latitude Deg 32 31 34 32 33 30 32 32 34 31 30 31 33 34 31 33 31 30 33 43 42 40 40 43 40 41 Min 31 41 3 48 2 41 57 23 8 5 52 14 39 17 11 39 52 47 13 22 50 52 46 29 58 45 Sec 6.60 34.80 52.56 40.32 9.24 53.88 0.72 13.56 12.48 56.04 22.08 43.08 14.76 57.12 7.08 32.04 49.08 6.72 50.52 10.20 20.04 40.80 37.56 17.88 26.40 11.52 7 of 35 ------- OWOW99 Site-ID OWOW99-1090 OWOW99-1432 OWOW99-0079 OWOW99-0127 OWOW99-0177 OWOW99-0553 OWOW99-0554 OWOW99-0627 OWOW99-0904 OWOW99-1028 OWOW99-1452 OWOW99-0015 OWOW99-0041 OWOW99-0091 OWOW99-0115 OWOW99-0140 OWOW99-0190 OWOW99-0491 OWOW99-0515 OWOW99-0916 OWOW99-0990 OWOW99-1065 OWOW99-1115 OWOW99-1390 OWOW99-1415 OWOW99-1441 Long. DD -94.2594 -94.8721 -117.0784 -111.1113 -112.9475 -114.6393 -116.8576 -111.3329 -115.9208 -115.8469 -111.5860 -88.7835 -88.6600 -89.2082 -89.8931 -90.6031 -89.3736 -87.5327 -89.5855 -89.3615 -89.8377 -88.9741 -90.2936 -89.9905 -90.0621 -88.2174 Lat. DD 41.5498 42.9460 44.6758 43.2436 42.7355 43.7787 48.5679 42.0037 45.0938 45.0996 42.9042 37.7733 41.6475 41.0500 39.4512 41.4549 38.5090 41.6645 40.5838 41.2039 38.2833 38.0812 40.1154 40.3401 38.9224 41.2945 Lake Name Diamondhead Lake Rush Lake Brownlee Reservoir PALISADES RES Priest Lake Bear Lake Blackfoot Reservoir Unnamed lake Buck Lake Unnamed lake Otter Lake Unnamed lake Unnamed lake Wolf Lake Unnamed lake Unnamed lake Unnamed lake Rend Lake Crane Lake McHarry Pond Unnamed lake Unnamed lake St. IA IA ID ID ID ID ID ID ID ID ID IL IL IL IL IL IL IL IL IL IL IL IL IL IL IL County GUTHRIE PALO ALTO WASHINGTON BONNEVILLE POWER ELAINE BONNER VALLEY VALLEY CARIBOU WILLIAMSON DEKALB MARSHALL MACOUPIN ROCK ISLAND CLINTON COOK TAZEWELL PUTNAM ST CLAIR FRANKLIN MASON MASON MADISON WILL Lake Area (ha) 40 178 6071 6062 6 8 9454 28329 3 3 6475 6 4 2 126 2 7 323 17 31 4 833 73 6 5 25 Yr. 3 4 1 1 1 2 2 2 3 3 4 1 1 1 1 1 1 2 2 3 3 3 3 4 4 4 Longitude Deg 94 94 117 111 112 114 116 111 115 115 111 88 88 89 89 90 89 87 89 89 89 88 90 89 90 88 Min 15 52 4 6 56 38 51 19 55 50 35 47 39 12 53 36 22 31 35 21 50 58 17 59 3 13 Sec 33.84 19.56 42.35 40.68 50.86 21.44 27.50 58.48 14.81 48.88 9.67 0.60 36.00 29.52 35.16 11.16 24.96 57.72 7.80 41.40 15.72 26.76 36.96 25.80 43.56 2.64 Latitude Deg 41 42 44 43 42 43 48 42 45 45 42 37 41 41 39 41 38 41 40 41 38 38 40 40 38 41 Min 32 56 40 14 44 46 34 0 5 5 54 46 38 2 27 27 30 39 35 12 16 4 6 20 55 17 Sec 59.28 45.60 32.74 36.96 7.94 43.39 4.37 13.32 37.50 58.45 15.01 23.88 51.00 60.00 4.32 17.64 32.40 52.20 1.68 14.04 59.88 52.32 55.44 24.36 20.64 40.20 8 of 35 ------- OWOW99 Site-ID OWOW99-1465 OWOW99-1565 OWOW99-0141 OWOW99-0191 OWOW99-0216 OWOW99-0466 OWOW99-0590 OWOW99-0616 OWOW99-0940 OWOW99-0941 OWOW99-1416 OWOW99-1516 OWOW99-1541 OWOW99-0044 OWOW99-0119 OWOW99-0168 OWOW99-0218 OWOW99-0568 OWOW99-0619 OWOW99-0668 OWOW99-0943 OWOW99-0993 OWOW99-1019 OWOW99-1119 OWOW99-1368 OWOW99-1519 Long. DD -88.5078 -89.4784 -86.7549 -85.5530 -86.3262 -85.8336 -87.5286 -85.9425 -87.2352 -85.6889 -86.3382 -85.0236 -86.9530 -97.7495 -96.7013 -94.7718 -95.8411 -95.9745 -98.4462 -96.2702 -96.5009 -98.0562 -99.2180 -95.6010 -95.1619 -98.2340 Lat. DD 37.7370 37.8187 39.7330 40.7298 39.0996 41.2229 39.0672 39.9282 39.0350 38.7726 41.6112 41.6268 40.0349 38.4030 39.4570 38.0787 38.2688 38.5312 37.9214 38.4313 37.3790 37.9161 39.5027 39.5024 38.5113 39.1122 Lake Name Unnamed lake Kinkaid Lake Baire Lake Winona Lake Turtle Creek Reservoir Geist Reservoir Round Lake Hardy Lake (gravel pit) Fox Lake Unnamed lake Tuttle Creek Lake St. IL IL IN IN IN IN IN IN IN IN IN IN IN KS KS KS KS KS KS KS KS KS KS KS KS KS County SALINE JACKSON PUTNAM HUNTINGTON BROWN KOSCIUSKO SULLIVAN HAMILTON GREENE SCOTT ST JOSEPH STEUBEN MONTGOMERY MCPHERSON POTTAWATOMIE LINN COFFEY LYON RENO LYON ELK RENO ROOKS JACKSON FRANKLIN LINCOLN Lake Area (ha) 8 972 3 102 59 216 606 683 8 316 18 53 5 12 2153 2 3 2 6 1 2 2 2 5 10 3 Yr. 4 4 1 1 1 2 2 2 3 3 4 4 4 1 1 1 1 2 2 2 3 3 3 3 4 4 Longitude Deg 88 89 86 85 86 85 87 85 87 85 86 85 86 97 96 94 95 95 98 96 96 98 99 95 95 98 Min 30 28 45 33 19 50 31 56 14 41 20 1 57 44 42 46 50 58 26 16 30 3 13 36 9 14 Sec 28.08 42.24 17.64 10.80 34.32 0.96 42.96 33.00 6.72 20.04 17.52 24.96 10.80 58.20 4.68 18.48 27.96 28.20 46.32 12.72 3.24 22.32 4.80 3.60 42.84 2.40 Latitude Deg 37 37 39 40 39 41 39 39 39 38 41 41 40 38 39 38 38 38 37 38 37 37 39 39 38 39 Min 44 49 43 43 5 13 4 55 2 46 36 37 2 24 27 4 16 31 55 25 22 54 30 30 30 6 Sec 13.20 7.32 58.80 47.28 58.56 22.44 1.92 41.52 6.00 21.36 40.32 36.48 5.64 10.80 25.20 43.32 7.68 52.32 17.04 52.68 44.40 57.96 9.72 8.64 40.68 43.92 9 of 35 ------- OWOW99 Site-ID OWOW99-1568 OWOW99-0037 OWOW99-0465 OWOW99-0640 OWOW99-0641 OWOW99-1012 OWOW99-1062 OWOW99-1361 OWOW99-0024 OWOW99-0074 OWOW99-0124 OWOW99-0149 OWOW99-0173 OWOW99-0174 OWOW99-0199 OWOW99-0224 OWOW99-0474 OWOW99-0549 OWOW99-0575 OWOW99-0599 OWOW99-0649 OWOW99-0674 OWOW99-0999 OWOW99-1048 OWOW99-1074 OWOW99-1099 Long. DD -95.6120 -86.1933 -88.4942 -85.6473 -84.7146 -85.2710 -84.7791 -88.1218 -89.8980 -92.3439 -90.1274 -93.4958 -93.3868 -93.4135 -93.0480 -89.8645 -89.7188 -91.9919 -89.5451 -91.6058 -91.5507 -89.2912 -90.5717 -93.7341 -93.4158 -92.0605 Lat. DD 37.8871 37.3077 37.2822 37.7978 37.6850 37.2335 36.9740 37.0234 29.6683 31.5566 30.1925 32.1731 32.4381 30.8376 29.9418 30.6719 30.1291 31.6677 29.4416 30.7783 29.9396 29.9980 29.9208 32.3496 30.2565 32.5879 Lake Name Unknown Unknown Lake Ponchartrain Unknown Lake Bisteneau Unknown Unknown Unknown Lake St. Catherine? Unknown Allen Bay Unknown Unknown Indian Mound Bay? Lac des Allemands Unknown Unknown Unknown St. KS KY KY KY KY KY KY KY LA LA LA LA LA LA LA LA LA LA LA LA LA LA LA LA LA LA County WOODSON EDMONSON LIVINGSTON NELSON BOYLE ADAIR PULASKI LYON PLAQUEMINES GRANT RED RIVER WEBSTER BEAUREGARD CAMERON WASHINGTON ORLEANS CATAHOULA PLAQUEMINES POINTE COUPEE ST MARY ST BERNARD CADDO CALCASIEU OUACHITA Lake Area (ha) 2 808 13 3 1084 3191 231 8 4 11 ##### 8 6283 5 602 34 3109 19 16 55 10 82 5957 8 64 6 Yr. 4 1 2 2 2 3 3 4 1 1 1 1 1 1 1 1 2 2 2 2 2 2 3 3 3 3 Longitude Deg 95 86 88 85 84 85 84 88 89 92 90 93 93 93 93 89 89 91 89 91 91 89 90 93 93 92 Min 36 11 29 38 42 16 46 7 53 20 7 29 23 24 2 51 43 59 32 36 33 17 34 44 24 3 Sec 43.20 35.88 39.12 50.28 52.56 15.60 44.76 18.48 52.80 38.04 38.46 44.88 12.48 48.60 52.80 52.20 7.68 30.84 42.36 20.88 2.52 28.32 18.05 2.76 56.88 37.80 Latitude Deg 37 37 37 37 37 37 36 37 29 31 30 32 32 30 29 30 30 31 29 30 29 29 29 32 30 32 Min 53 18 16 47 41 14 58 1 40 33 11 10 26 50 56 40 7 40 26 46 56 59 55 20 15 35 Sec 13.56 27.72 55.92 52.08 6.00 0.60 26.40 24.24 5.88 23.76 33.14 23.16 17.16 15.36 30.48 18.84 44.76 3.72 29.76 41.88 22.56 52.80 14.95 58.56 23.40 16.44 10 of 35 ------- OWOW99 Site-ID OWOW99-1124 OWOW99-1374 OWOW99-1375 OWOW99-1424 OWOW99-1474 OWOW99-1499 OWOW99-1548 OWOW99-1549 OWOW99-0017 OWOW99-0043 OWOW99-0467 OWOW99-0493 OWOW99-0567 OWOW99-0592 OWOW99-0992 OWOW99-1017 OWOW99-1443 OWOW99-0563 OWOW99-0564 OWOW99-1439 OWOW99-0042 OWOW99-0092 OWOW99-0166 OWOW99-0192 OWOW99-0210 OWOW99-0217 Long. DD -89.3347 -92.3550 -89.6034 -89.8663 -91.7324 -91.5497 -91.9289 -91.2212 -71.1075 -71.0101 -70.0928 -71.9988 -72.3087 -71.8687 -71.6047 -70.6276 -71.7692 -76.3694 -75.8240 -79.3215 -69.8635 -68.2270 -68.0095 -70.5879 -69.3678 -70.8049 Lat. DD 29.8557 30.7519 29.5372 29.6994 30.7120 31.4483 32.8645 32.0874 41.7031 42.6178 41.7239 42.5272 42.4015 42.1353 42.2435 41.3523 42.5272 38.8416 38.0482 39.5043 45.1845 44.8762 44.9731 44.1533 45.8203 44.0539 Lake Name Christmas Camp Lake? Miller's Lake California Bav? Unknown Unknown Old River Lake Bussy Brake Lake Bruin North Watuppa Pond Prichards Pond Seymour Pond Bents Pond QUABBIN RES Carbuncle Pond Westboro Reservoir Big Homer Pond Rockwell Pond Holligans Snooze Inlet Annemessex River Deep Creek Lake Heald Ponds Lower Middle Branch Pond Stiles Lake Little Pond Raaaed Lake Moose Pond St. LA LA LA LA LA LA LA LA MA MA MA MA MA MA MA MA MA MD MD MD ME ME ME ME ME ME County ST BERNARD EVANGELINE PLAQUEMINES PLAQUEMINES POINTE COUPEE CONCORDIA MOREHOUSE TENSAS BRISTOL ESSEX BARNSTABLE WORCESTER WORCESTER WORCESTER WORCESTER DUKES WORCESTER QUEEN ANNES SOMERSET GARRETT SOMERSET HANCOCK HANCOCK OXFORD PISCATAQUIS CUMBERLAND Lake Area (ha) 21 1246 1302 6 16 3 848 7 674 5 69 9 9536 4 1 13 4 10 1710 1449 9 104 17 11 1047 679 Yr. 3 4 4 4 4 4 4 4 1 1 2 2 2 2 3 3 4 2 2 4 1 1 1 1 1 1 Longitude Deg 89 92 89 89 91 91 91 91 71 71 70 71 72 71 71 70 71 76 75 79 69 68 68 70 69 70 Min 20 21 36 51 43 32 55 13 6 0 5 59 18 52 36 37 46 22 49 19 51 13 0 35 22 48 Sec 4.92 18.00 12.24 58.68 56.64 58.92 44.04 16.32 27.00 36.36 34.08 55.68 31.32 7.32 16.92 39.36 9.12 9.84 26.40 17.40 48.60 37.20 34.20 16.44 4.08 17.64 Latitude Deg 29 30 29 29 30 31 32 32 41 42 41 42 42 42 42 41 42 38 38 39 45 44 44 44 45 44 Min 51 45 32 41 42 26 51 5 42 37 43 31 24 8 14 21 31 50 2 30 11 52 58 9 49 3 Sec 20.52 6.84 13.92 57.84 43.20 53.88 52.20 14.64 11.16 4.08 26.04 37.92 5.40 7.08 36.60 8.28 37.92 29.76 53.52 15.48 4.20 34.32 23.16 11.88 13.08 14.04 11 of 35 ------- OWOW99 Site-ID OWOW99-0492 OWOW99-0516 OWOW99-0566 OWOW99-0617 OWOW99-0635 OWOW99-0642 OWOW99-0660 OWOW99-0667 OWOW99-0917 OWOW99-0935 OWOW99-0966 OWOW99-0967 OWOW99-1041 OWOW99-1067 OWOW99-1366 OWOW99-1391 OWOW99-1392 OWOW99-1442 OWOW99-1460 OWOW99-1560 OWOW99-0009 OWOW99-0014 OWOW99-0016 OWOW99-0116 OWOW99-0159 Long. DD -69.7220 -67.8207 -68.4982 -70.3992 -68.7143 -69.4533 -69.2984 -70.8288 -67.4489 -69.0874 -67.6423 -70.8544 -68.9015 -70.0304 -69.1132 -68.3404 -70.8790 -70.2829 -69.0628 -69.8704 -85.0115 -83.4149 -86.3480 -85.3849 -87.0669 Lat. DD 45.6786 45.1594 44.6483 44.0212 47.1056 44.0098 46.1063 44.8868 44.7863 46.5076 45.6216 43.9330 45.6875 44.4858 44.2628 45.6371 43.6124 45.6192 46.3064 45.9150 45.3005 43.0991 41.9271 42.3976 46.3338 Lake Name Moosehead Lake Little River Lake Green Lake Middle Range Pond Wallagrass Lakes McCurdy Pond Cuxabexis Lake Mooselookmeguntic Lake Hadley Lake Peaked Mountain Pond Spednik Lake Unnamed Pemadumcook Lake Parker Pond Megunticook Lake Unnamed Mud Pond Wood Pond Chandler Pond Seboomook Lake Walloon Lake West Lake #1 Lake Chapin Torch Lake Dorsev Lake St. ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME ME Ml Ml Ml Ml Ml County WASHINGTON HANCOCK ANDROSCOGGIN AROOSTOOK LINCOLN PISCATAQUIS OXFORD WASHINGTON PISCATAQUIS WASHINGTON OXFORD PISCATAQUIS KENNEBEC WALDO AROOSTOOK YORK SOMERSET PISCATAQUIS SOMERSET EMMET LAPEER BERRIEN KALAMAZOO ALGER Lake Area (ha) 30308 29 1267 15 100 80 247 6597 680 5 5571 7 7453 611 574 5 8 819 52 2571 1832 1 220 13 16 Yr. 2 2 2 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 4 1 1 1 1 1 Longitude Deg 69 67 68 70 68 69 69 70 67 69 67 70 68 70 69 68 70 70 69 69 85 83 86 85 87 Min 43 49 29 23 42 27 17 49 26 5 38 51 54 1 6 20 52 16 3 52 0 24 20 23 4 Sec 19.09 14.52 53.52 57.12 51.48 11.88 54.24 43.68 56.04 14.64 32.28 15.84 5.40 49.44 47.52 25.44 44.40 58.44 46.08 13.44 41.40 53.64 52.80 5.64 0.84 Latitude Deg 45 45 44 44 47 44 46 44 44 46 45 43 45 44 44 45 43 45 46 45 45 43 41 42 46 Min 40 9 38 1 6 0 6 53 47 30 37 55 41 29 15 38 36 37 18 54 18 5 55 23 20 Sec 43.10 33.84 53.88 16.32 20.16 35.28 22.68 12.48 10.68 27.36 17.76 58.80 15.00 8.88 46.08 13.56 44.64 9.12 23.04 54.00 1.80 56.76 37.56 51.36 1.68 12 of 35 ------- OWOW99 Site-ID OWOW99-0184 OWOW99-0189 OWOW99-0209 OWOW99-0214 OWOW99-0459 OWOW99-0464 OWOW99-0509 OWOW99-0534 OWOW99-0539 OWOW99-0589 OWOW99-0591 OWOW99-0609 OWOW99-0634 OWOW99-0639 OWOW99-0659 OWOW99-0664 OWOW99-0934 OWOW99-0939 OWOW99-0984 OWOW99-0989 OWOW99-1016 OWOW99-1064 OWOW99-1116 OWOW99-1384 OWOW99-1414 OWOW99-1459 Long. DD -85.5683 -85.3889 -85.9940 -84.7831 -84.6654 -83.5643 -88.0520 -88.8515 -85.2538 -84.2805 -86.1248 -89.1549 -85.3152 -84.7165 -84.7507 -84.2082 -86.0872 -86.1309 -83.5097 -83.5418 -85.5184 -85.3592 -85.5206 -87.9626 -84.2279 -86.0181 Lat. DD 44.5887 44.0069 46.2242 42.5946 45.4599 42.6691 46.5487 46.8884 43.3313 44.4161 42.2524 46.1527 44.9782 44.3499 45.6852 42.1467 46.5590 44.0559 45.0968 42.6689 41.8123 43.1538 42.1948 46.6857 41.7327 44.8708 Lake Name Hackman Lake Boot Lake TPool Unnamed Burt Lake White Lake Mud Lake Lake Roland Spring Lake Horseshoe Lake Dyer Lake Birch Lake Torch Lake Houghton Lake Lake Paradise Norvell Lake Cloverleaf Lake Mud Lake Seven Mile Pond Grass Lake Tamarack Lake Little Wabasis Lake Long Lake Lake Margaret Glen Lake St. Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml Ml County GRAND TRAVERSE OSCEOLA SCHOOLCRAFT EATON CHEBOYGAN OAKLAND MARQUETTE HOUGHTON MONTCALM OGEMAW VAN BUREN GOGEBIC ANTRIM ROSCOMMON EMMET JACKSON ALGER MASON ALPENA OAKLAND ST JOSEPH KENT KALAMAZOO MARQUETTE LENAWEE LEELANAU Lake Area (ha) 7 3 95 6 0 198 2 107 22 14 12 84 7503 8068 767 12 5 2 556 15 52 14 198 52 6 560 Yr. 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 Longitude Deg 85 85 85 84 84 83 88 88 85 84 86 89 85 84 84 84 86 86 83 83 85 85 85 87 84 86 Min 34 23 59 46 39 33 3 51 15 16 7 9 18 42 45 12 5 7 30 32 31 21 31 57 13 1 Sec 5.88 20.04 38.40 59.16 55.58 51.48 7.20 5.40 13.68 49.80 29.28 17.64 54.72 59.40 2.52 29.52 13.92 51.24 34.92 30.48 6.24 33.12 14.16 45.36 40.44 5.16 Latitude Deg 44 44 46 42 45 42 46 46 43 44 42 46 44 44 45 42 46 44 45 42 41 43 42 46 41 44 Min 35 0 13 35 27 40 32 53 19 24 15 9 58 20 41 8 33 3 5 40 48 9 11 41 43 52 Sec 19.32 24.84 27.12 40.56 35.78 8.76 55.32 18.24 52.68 57.96 8.64 9.72 41.52 59.64 6.72 48.12 32.40 21.24 48.48 8.04 44.28 13.68 41.28 8.52 57.72 14.88 13 of 35 ------- OWOW99 Site-ID OWOW99-1464 OWOW99-1489 OWOW99-1534 OWOW99-1559 OWOW99-1564 OWOW99-0005 OWOW99-0010 OWOW99-0031 OWOW99-0032 OWOW99-0033 OWOW99-0035 OWOW99-0055 OWOW99-0057 OWOW99-0081 OWOW99-0083 OWOW99-0085 OWOW99-0106 OWOW99-0110 OWOW99-0130 OWOW99-0132 OWOW99-0155 OWOW99-0157 OWOW99-0180 OWOW99-0182 OWOW99-0183 OWOW99-0205 Long. DD -83.5503 -84.5111 -89.5863 -83.6934 -83.8948 -95.1647 -90.9426 -94.9994 -96.3941 -93.5870 -91.1740 -93.5770 -95.6236 -95.9084 -94.2868 -90.4943 -95.4026 -92.8239 -92.1057 -95.8216 -94.2687 -94.6157 -94.2727 -93.5168 -92.7562 -94.5317 Lat. DD 43.1634 43.1184 46.5082 46.0584 42.5038 47.3415 48.0215 46.8863 45.5205 45.4859 48.0688 47.1810 45.7808 46.7805 46.3518 47.8652 47.1965 48.5579 47.3197 45.6412 46.4872 45.3749 46.9586 44.7412 46.3802 47.4232 Lake Name Unnamed Goaebic Lake Chenango Lake (Lime Lake) LaSalle Mora Long Unnamed Cantlin Kekekabic Pokeaama Lake Unnamed Fox White Sand Dick Glanders Namakan Lake Linwood Lake?? Unnamed Hubert Rice Woman O'Dowd Sturaeon Cass Lake St. Ml Ml Ml Ml Ml MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN County GENESEE GRATIOT CHIPPEWA LIVINGSTON HUBBARD COOK HUBBARD BIG STONE SHERBURNE LAKE ITASCA DOUGLAS BECKER CROW WING COOK CLEARWATER ST LOUIS STEVENS CROW WING STEARNS CASS SCOTT PINE Lake Area (ha) 2 1375 5170 29 12 90 94 784 6 41 691 6313 8 56 159 53 20 5686 3 8 511 618 2396 118 666 12050 Yr. 4 4 4 4 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Longitude Deg 83 84 89 83 83 95 90 94 96 93 91 93 95 95 94 90 95 92 92 95 94 94 94 93 92 94 Min 33 30 35 41 53 9 56 59 23 35 10 34 37 54 17 29 24 49 6 49 16 36 16 31 45 31 Sec 1.08 39.96 10.50 36.24 41.28 52.92 33.36 57.84 38.76 13.20 26.40 37.20 24.96 30.24 12.48 39.48 9.36 25.93 20.52 17.76 7.32 56.52 21.72 0.48 22.32 53.94 Latitude Deg 43 43 46 46 42 47 48 46 45 45 48 47 45 46 46 47 47 48 47 45 46 45 46 44 46 47 Min 9 7 30 3 30 20 1 53 31 29 4 10 46 46 21 51 11 33 19 38 29 22 57 44 22 25 Sec 48.24 6.24 29.56 30.24 13.68 29.40 17.40 10.68 13.80 9.24 7.68 51.60 50.88 49.80 6.48 54.72 47.40 28.51 10.92 28.32 13.92 29.64 30.96 28.32 48.72 23.48 14 of 35 ------- OWOW99 Site-ID OWOW99-0207 OWOW99-0455 OWOW99-0460 OWOW99-0481 OWOW99-0482 OWOW99-0483 OWOW99-0485 OWOW99-0505 OWOW99-0507 OWOW99-0530 OWOW99-0532 OWOW99-0555 OWOW99-0556 OWOW99-0557 OWOW99-0559 OWOW99-0581 OWOW99-0583 OWOW99-0585 OWOW99-0605 OWOW99-0607 OWOW99-0630 OWOW99-0632 OWOW99-0633 OWOW99-0655 OWOW99-0905 OWOW99-0906 Long. DD -93.2741 -93.5638 -91.5581 -95.5381 -96.0942 -94.0019 -92.0778 -96.0091 -94.1022 -92.9112 -94.5663 -95.8735 -94.4671 -95.3693 -90.6578 -95.7940 -92.8785 -93.1006 -92.2737 -95.3151 -93.9130 -93.1093 -92.8199 -94.8418 -95.9718 -95.4222 Lat. DD 43.7920 47.5288 47.6143 47.0775 45.3626 45.5746 48.2927 46.3972 45.3217 46.8136 45.5608 46.6991 43.6875 46.0890 47.9947 47.4786 45.2609 48.2787 46.9391 45.5396 46.4961 44.8687 46.3146 47.6093 48.3293 46.3986 Lake Name Geneva Pickerel South McDouaal Many Point Unnamed Unnamed LAC LA CROIX Wolf Bass Moberg Lauer Unnamed Buffalo Unnamed Trurno Unnamed Unnamed Unnamed Fish Lake Reservoir Swenoda Agate Unnamed First Fox Mud Lake East Leaf St. MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN County FREEBORN ITASCA LAKE BECKER SWIFT BENTON ST LOUIS OTTER TAIL WRIGHT ST LOUIS STEARNS OTTER TAIL MARTIN DOUGLAS COOK MAHNOMEN WASHINGTON KOOCHICHING ST LOUIS POPE CROW WING DAKOTA PINE BELTRAMI OTTER TAIL Lake Area (ha) 694 16 113 677 11 2 5769 16 86 14 5 5 103 7 14 8 21 1 1214 117 66 2 31 64 9591 170 Yr. 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 Longitude Deg 93 93 91 95 96 94 92 96 94 92 94 95 94 95 90 95 92 93 92 95 93 93 92 94 95 95 Min 16 33 33 32 5 0 4 0 6 54 33 52 28 22 39 47 52 6 16 18 54 6 49 50 58 25 Sec 26.76 49.68 29.16 17.16 39.12 6.84 40.08 32.76 7.92 40.32 58.68 24.60 1.56 9.48 28.08 38.40 42.60 2.16 25.32 54.36 46.80 33.48 11.64 30.48 18.48 19.92 Latitude Deg 43 47 47 47 45 45 48 46 45 46 45 46 43 46 47 47 45 48 46 45 46 44 46 47 48 46 Min 47 31 36 4 21 34 17 23 19 48 33 41 41 5 59 28 15 16 56 32 29 52 18 36 19 23 Sec 31.20 43.68 51.48 39.00 45.36 28.56 33.72 49.92 18.12 48.96 38.88 56.76 15.00 20.40 40.92 42.96 39.24 43.32 20.76 22.56 45.96 7.32 52.56 33.48 45.55 54.96 15 of 35 ------- OWOW99 Site-ID OWOW99-0908 OWOW99-0910 OWOW99-0930 OWOW99-0932 OWOW99-0933 OWOW99-0955 OWOW99-0957 OWOW99-0980 OWOW99-0985 OWOW99-1006 OWOW99-1008 OWOW99-1010 OWOW99-1030 OWOW99-1032 OWOW99-1034 OWOW99-1055 OWOW99-1057 OWOW99-1080 OWOW99-1082 OWOW99-1110 OWOW99-1355 OWOW99-1357 OWOW99-1359 OWOW99-1380 OWOW99-1382 OWOW99-1410 Long. DD -94.6005 -90.9841 -95.5579 -91.6562 -93.6431 -95.9853 -95.0638 -95.0251 -91.2914 -96.1832 -93.0521 -91.7537 -96.2475 -93.6363 -93.0806 -94.3916 -93.8774 -93.4365 -95.6831 -92.3073 -95.8561 -94.4259 -94.9752 -95.7993 -94.8427 -91.9695 Lat. DD 46.2537 48.0774 47.6234 44.0415 46.2381 46.0809 45.2362 47.9620 47.8110 47.6754 45.1318 47.8983 46.8293 44.9095 48.5981 47.1557 44.2542 47.2130 45.8481 47.8681 46.5908 44.9814 49.3608 46.3063 45.1832 48.0107 Lake Name Shamineau Howard Lake Unnamed Winona Miles Lacs Four Mile Florida Lower Red Lake Isabella Unnamed Amelia White Iron Unnamed Minnetonka Leech Lake Washington Unnamed Unnamed Vermilion Lake McCollume Belle North Turtle Diamond Slim St. MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN MN County MORRISON COOK POLK WINONA MILLE LACS GRANT KANDIYOHI LAKE POLK ANOKA LAKE CLAY HENNEPIN ST LOUIS LESUEUR ITASCA DOUGLAS OTTER TAIL MEEKER LAKE OF THE WOODS OTTER TAIL KANDIYOHI ST LOUIS Lake Area (ha) 548 69 13 32 51700 79 211 0 667 7 50 2404 12 1700 1 44280 582 7 14 19875 6 362 114 601 626 131 Yr. 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 Longitude Deg 94 90 95 91 93 95 95 95 91 96 93 91 96 93 93 94 93 93 95 92 95 94 94 95 94 91 Min 36 59 33 39 38 59 3 1 17 10 3 45 14 38 4 23 52 26 40 18 51 25 58 47 50 58 Sec 1.80 2.76 28.44 22.32 35.16 7.08 49.68 30.29 29.04 59.52 7.56 13.32 51.00 10.68 50.16 29.69 38.64 11.40 59.16 26.17 21.96 33.24 30.72 57.48 33.72 10.20 Latitude Deg 46 48 47 44 46 46 45 47 47 47 45 47 46 44 48 47 44 47 45 47 46 44 49 46 45 48 Min 15 4 37 2 14 4 14 57 48 40 7 53 49 54 35 9 15 12 50 52 35 58 21 18 10 0 Sec 13.32 38.64 24.24 29.40 17.16 51.24 10.32 43.02 39.60 31.44 54.48 53.88 45.48 34.20 53.16 20.48 15.12 46.80 53.16 5.20 26.88 53.04 38.88 22.68 59.52 38.52 16 of 35 ------- OWOW99 Site-ID OWOW99-1430 OWOW99-1431 OWOW99-1435 OWOW99-1455 OWOW99-1457 OWOW99-1458 OWOW99-1480 OWOW99-1482 OWOW99-1506 OWOW99-1508 OWOW99-1510 OWOW99-1530 OWOW99-1532 OWOW99-1555 OWOW99-0040 OWOW99-0215 OWOW99-0490 OWOW99-0518 OWOW99-0543 OWOW99-0618 OWOW99-0665 OWOW99-0912 OWOW99-0915 OWOW99-0968 OWOW99-1015 OWOW99-1043 Long. DD -95.2037 -95.7495 -91.0591 -93.7461 -92.3071 -92.5169 -94.7573 -93.7620 -95.6548 -93.7467 -91.4933 -93.5769 -95.3562 -95.1158 -91.0894 -89.6157 -92.1693 -92.9303 -93.3961 -93.6900 -91.3675 -89.1714 -91.6021 -91.5418 -90.4555 -94.3974 Lat. DD 48.9700 46.4793 48.1618 46.6502 44.5155 46.2546 47.1668 44.4942 46.9790 45.1509 47.9748 47.4910 45.9641 47.7441 38.8129 37.2404 38.9230 38.5295 36.5590 37.3760 38.0854 36.7866 39.2285 37.7095 38.7693 38.7750 Lake Name Lake of the Woods Dead Unnamed Blind Lake Pepin Unnamed Kabekona Thomas Flat Charlotte Ennis Spider Carlos Unnamed TABLE ROCK L St. MN MN MN MN MN MN MN MN MN MN MN MN MN MN MO MO MO MO MO MO MO MO MO MO MO MO County OTTER TAIL LAKE AITKIN GOODHUE PINE HUBBARD LESUEUR BECKER WRIGHT LAKE ITASCA DOUGLAS BELTRAMI WARREN CAPE GIRARDEAU BOONE MORGAN STONE DADE CRAWFORD MISSISSIPPI AUDRAIN DENT ST LOUIS CASS Lake Area (ha) ##### 2988 2 120 5075 10 975 48 741 94 9 546 1040 6 10 128 9 4 12410 3 2 8 8 2 2 12 Yr. 4 4 4 4 4 4 4 4 4 4 4 4 4 4 1 1 2 2 2 2 2 3 3 3 3 3 Longitude Deg 95 95 91 93 92 92 94 93 95 93 91 93 95 95 91 89 92 92 93 93 91 89 91 91 90 94 Min 12 44 3 44 18 31 45 45 39 44 29 34 21 6 5 36 10 55 23 41 22 10 36 32 27 23 Sec 13.25 58.20 32.76 45.96 25.56 0.84 26.28 43.20 17.28 48.12 35.88 36.84 22.32 56.88 21.84 56.52 9.48 49.08 45.96 24.00 3.00 17.04 7.56 30.48 19.80 50.64 Latitude Deg 48 46 48 46 44 46 47 44 46 45 47 47 45 47 38 37 38 38 36 37 38 36 39 37 38 38 Min 58 28 9 39 30 15 10 29 58 9 58 29 57 44 48 14 55 31 33 22 5 47 13 42 46 46 Sec 12.07 45.48 42.48 0.72 55.80 16.56 0.48 39.12 44.40 3.24 29.28 27.60 50.76 38.76 46.44 25.44 22.80 46.20 32.40 33.60 7.44 11.76 42.60 34.20 9.48 30.00 17 of 35 ------- OWOW99 Site-ID OWOW99-1068 OWOW99-1393 OWOW99-1437 OWOW99-1440 OWOW99-1490 OWOW99-1515 OWOW99-0071 OWOW99-0098 OWOW99-0146 OWOW99-0172 OWOW99-0472 OWOW99-0521 OWOW99-0522 OWOW99-0546 OWOW99-0572 OWOW99-0621 OWOW99-0624 OWOW99-0672 OWOW99-0949 OWOW99-0997 OWOW99-1047 OWOW99-1096 OWOW99-1122 OWOW99-1372 OWOW99-1397 OWOW99-1422 Long. DD -94.5328 -93.5719 -89.4497 -91.7100 -92.0686 -91.9827 -91.1601 -88.5441 -89.8375 -90.8814 -88.1104 -90.4070 -90.1623 -90.9335 -89.5842 -90.2024 -90.7826 -89.7129 -89.0588 -89.8621 -88.9294 -89.7340 -88.7112 -90.3281 -90.1054 -89.7740 Lat. DD 37.2897 38.1700 36.5535 39.5129 40.0317 38.9651 33.5082 32.5750 33.4770 32.7220 34.8551 34.0076 32.2687 33.2578 31.6186 33.5276 32.0304 34.4486 30.8977 34.1474 32.8226 33.8319 33.7215 32.2211 32.5856 34.0039 Lake Name TRUMAN RES Mark Twain Lake Sardis Lake Enid Lake Grenada Lake St. MO MO MO MO MO MO MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS County JASPER ST CLAIR NEW MADRID RALLS KNOX CALLAWAY WASHINGTON LAUDERDALE CARROLL SHARKEY TISHOMINGO TALLAHATCHIE RANKIN WASHINGTON COVINGTON LEFLORE CLAIBORNE STONE NESHOBA MONROE HINDS MADISON YALOBUSHA Lake Area (ha) 14 9246 10 3551 4 9 47 12 50 10 636 75 4 6 3 1016 37 23684 1 11230 4 26154 5 2 19 6 Yr. 3 4 4 4 4 4 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 4 4 4 Longitude Deg 94 93 89 91 92 91 91 88 89 90 88 90 90 90 89 90 90 89 89 89 88 89 88 90 90 89 Min 31 34 26 42 4 58 9 32 50 52 6 24 9 56 35 12 46 42 3 51 55 44 42 19 6 46 Sec 58.08 18.84 58.92 36.00 6.96 57.72 36.36 38.76 15.00 53.04 37.44 25.20 44.28 0.60 3.12 8.64 57.36 46.48 31.68 43.45 45.84 2.36 40.32 41.16 19.44 26.40 Latitude Deg 37 38 36 39 40 38 33 32 33 32 34 34 32 33 31 33 32 34 30 34 32 33 33 32 32 34 Min 17 10 33 30 1 57 30 34 28 43 51 0 16 15 37 31 1 26 53 8 49 49 43 13 35 0 Sec 22.92 12.00 12.60 46.44 54.12 54.36 29.52 30.00 37.20 19.20 18.36 27.36 7.32 28.08 6.96 39.36 49.44 55.03 51.72 50.68 21.36 54.80 17.40 15.96 8.16 14.04 18 of 35 ------- OWOW99 Site-ID OWOW99-1448 OWOW99-1546 OWOW99-1572 OWOW99-1574 OWOW99-0029 OWOW99-0053 OWOW99-0054 OWOW99-0084 OWOW99-0104 OWOW99-0129 OWOW99-0153 OWOW99-0178 OWOW99-0181 OWOW99-0454 OWOW99-0479 OWOW99-0558 OWOW99-0579 OWOW99-0604 OWOW99-0909 OWOW99-0952 OWOW99-1004 OWOW99-1029 OWOW99-1079 OWOW99-1104 OWOW99-1358 OWOW99-1404 Long. DD -89.3108 -90.0304 -90.0714 -89.5315 -111.2044 -108.1039 -113.7489 -106.7435 -107.4776 -113.3498 -113.1950 -104.6747 -104.5234 -113.9012 -111.5479 -106.4908 -111.5445 -115.2348 -108.5948 -111.2497 -111.9909 -115.6653 -113.8897 -115.4069 -106.0834 -113.2146 Lat. DD 31.3542 33.5995 34.7557 30.3291 48.3775 45.1706 48.6154 47.7335 47.1298 48.8880 44.9973 45.6234 47.2791 47.5570 47.4160 47.6953 46.3699 48.5864 48.3476 44.7870 48.0219 48.2210 48.1628 47.2692 47.8205 47.0855 Lake Name BIGHORN L FORT PECK RES L KOOCANUSA Hebgen Lake St. MS MS MS MS MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT MT County FORREST CARROLL DE SOTO HANCOCK LIBERTY BIGHORN GLACIER VALLEY GARFIELD GLACIER BEAVERHEAD CARTER DAWSON MISSOULA CASCADE GARFIELD BROADWATER LINCOLN ELAINE TETON LINCOLN FLATHEAD MINERAL MCCONE POWELL Lake Area (ha) 30 8 3249 2 1076 6943 1 98766 6 6 10 8 2055 23 20 2 196 11463 59 0 9 52 9 3 5 2 Yr. 4 4 4 4 1 1 1 1 1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 4 4 Longitude Deg 89 90 90 89 111 108 113 106 107 113 113 104 104 113 111 106 111 115 108 111 111 115 113 115 106 113 Min 18 1 4 31 12 6 44 44 28 20 11 40 31 54 32 29 32 14 35 14 59 39 53 24 5 12 Sec 38.88 49.44 17.04 53.40 15.84 14.04 56.04 36.60 39.36 59.28 42.00 28.92 24.24 4.32 52.44 26.88 40.20 5.28 41.28 58.74 27.24 55.08 22.92 24.84 0.24 52.56 Latitude Deg 31 33 34 30 48 45 48 47 47 48 44 45 47 47 47 47 46 48 48 44 48 48 48 47 47 47 Min 21 35 45 19 22 10 36 44 7 53 59 37 16 33 24 41 22 35 20 47 1 13 9 16 49 5 Sec 15.12 58.20 20.52 44.76 39.00 14.16 55.44 0.60 47.28 16.80 50.28 24.24 44.76 25.20 57.60 43.08 11.64 11.04 51.36 13.02 18.84 15.60 46.08 9.12 13.80 7.80 19 of 35 ------- OWOW99 Site-ID OWOW99-1429 OWOW99-1434 OWOW99-1504 OWOW99-1529 OWOW99-0062 OWOW99-0137 OWOW99-0139 OWOW99-0162 OWOW99-0164 OWOW99-0537 OWOW99-0611 OWOW99-0612 OWOW99-0962 OWOW99-1037 OWOW99-1112 OWOW99-1387 OWOW99-1389 OWOW99-0006 OWOW99-0030 OWOW99-0034 OWOW99-0105 OWOW99-0109 OWOW99-0131 OWOW99-0156 OWOW99-0456 Long. DD -112.4335 -107.2259 -111.6821 -114.0767 -81.4559 -80.4275 -76.4601 -79.0165 -78.0190 -80.9698 -83.3703 -78.9274 -79.8803 -78.4574 -77.2761 -78.6858 -77.2762 -100.0630 -98.8053 -103.9666 -98.9742 -100.2730 -100.9038 -99.9637 -99.0408 Lat. DD 47.9460 48.7055 45.4309 48.8717 35.3010 35.7108 35.7687 35.7732 36.5410 35.3508 35.4456 35.1360 35.7551 35.2586 34.7116 36.0778 36.1907 46.7390 48.2210 48.6934 48.2524 48.8584 46.9226 47.4209 47.1242 Lake Name Kings Mt. Reservoir Impoundment/ Grants Creek Phelps Lake B Everett Jordan Lake Lake Gaston Mt. Island Lake Impoundment/Oconoluft ee River Smith Lake Unnamed Unnamed Unnamed Unnamed Unnamed Long Lake Devils Lake St. MT MT MT MT NC NC NC NC NC NC NC NC NC NC NC NC NC ND ND ND ND ND ND ND ND County TETON PHILLIPS MADISON FLATHEAD CLEVELAND ROWAN CHATHAM WARREN MECKLENBURG SWAIN CUMBERLAND RANDOLPH SAMPSON ONSLOW GRANVILLE BERTIE KIDDER RAMSEY DIVIDE RAMSEY BOTTINEAU BURLEIGH WELLS STUTSMAN Lake Area (ha) 1296 231 1491 1 552 6 6718 5787 7951 1404 7 34 1 24 6 1 6 1300 7120 9 2196 5 20 37 3 Yr. 4 4 4 4 1 1 1 1 1 2 2 2 3 3 3 4 4 1 1 1 1 1 1 1 2 Longitude Deg 112 107 111 114 81 80 76 79 78 80 83 78 79 78 77 78 77 100 98 103 98 100 100 99 99 Min 26 13 40 4 27 25 27 0 1 58 22 55 52 27 16 41 16 3 48 57 58 16 54 57 2 Sec 0.60 33.24 55.56 36.12 21.24 39.00 36.18 59.40 8.40 11.28 13.08 38.64 49.08 26.64 33.96 8.88 34.32 46.80 19.08 59.76 27.12 22.80 13.68 49.32 26.88 Latitude Deg 47 48 45 48 35 35 35 35 36 35 35 35 35 35 34 36 36 46 48 48 48 48 46 47 47 Min 56 42 25 52 18 42 46 46 32 21 26 8 45 15 42 4 11 44 13 41 15 51 55 25 7 Sec 45.60 19.80 51.24 18.12 3.60 38.88 7.36 23.52 27.60 2.88 44.16 9.60 18.36 30.96 41.76 40.08 26.52 20.40 15.60 36.24 8.64 30.24 21.36 15.24 27.12 20 of 35 ------- OWOW99 Site-ID OWOW99-0480 OWOW99-0484 OWOW99-0506 OWOW99-0580 OWOW99-0584 OWOW99-0606 OWOW99-0656 OWOW99-0956 OWOW99-0981 OWOW99-1005 OWOW99-1009 OWOW99-1081 OWOW99-1105 OWOW99-1106 OWOW99-1109 OWOW99-1381 OWOW99-1405 OWOW99-1406 OWOW99-1409 OWOW99-1456 OWOW99-1505 OWOW99-1509 OWOW99-1531 OWOW99-0003 OWOW99-0065 OWOW99-0094 Long. DD -98.1807 -103.4168 -99.1740 -100.2759 -102.3091 -100.3287 -98.9907 -99.7841 -99.2248 -99.8568 -102.0806 -101.4909 -97.8659 -97.8346 -100.2276 -99.5410 -99.0504 -99.1631 -99.9233 -99.4723 -100.1783 -101.9556 -102.4403 -99.1880 -96.3294 -96.7822 Lat. DD 47.8124 48.2622 47.1297 47.8418 48.5432 47.7739 46.0214 47.0430 46.6055 48.5667 48.3434 47.9278 47.5268 46.7275 48.9943 47.2677 47.6458 46.9102 48.8529 46.1183 48.1937 48.2697 47.9289 42.2988 42.2335 41.2891 Lake Name St. ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND NE NE NE County NELSON WILLIAMS STUTSMAN SHERIDAN MOUNTRAIL SHERIDAN DICKEY KIDDER LOGAN ROLETTE MOUNTRAIL WARD STEELE BARNES BOTTINEAU KIDDER EDDY STUTSMAN ROLETTE MCINTOSH PIERCE MOUNTRAIL MOUNTRAIL HOLT THURSTON SAUNDERS Lake Area (ha) 13 60 11 24 6 8 12 1356 4 49 4 52 5 13 20 64 16 12 5 204 10 55 2 37 6 3 Yr. 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 1 1 1 Longitude Deg 98 103 99 100 102 100 98 99 99 99 102 101 97 97 100 99 99 99 99 99 100 101 102 99 96 96 Min 10 25 10 16 18 19 59 47 13 51 4 29 51 50 13 32 3 9 55 28 10 57 26 11 19 46 Sec 50.52 0.48 26.40 33.24 32.76 43.32 26.52 2.76 29.28 24.48 50.16 27.24 57.24 4.56 39.36 27.60 1.44 47.16 23.88 20.28 41.88 20.16 25.08 16.80 45.84 55.92 Latitude Deg 47 48 47 47 48 47 46 47 46 48 48 47 47 46 48 47 47 46 48 46 48 48 47 42 42 41 Min 48 15 7 50 32 46 1 2 36 34 20 55 31 43 59 16 38 54 51 7 11 16 55 17 14 17 Sec 44.64 43.92 46.92 30.48 35.52 26.04 17.04 34.80 19.80 0.12 36.24 40.08 36.48 39.00 39.48 3.72 44.88 36.72 10.44 5.88 37.32 10.92 44.04 55.68 0.60 20.76 21 of 35 ------- OWOW99 Site-ID OWOW99-0103 OWOW99-0453 OWOW99-0494 OWOW99-0578 OWOW99-0594 OWOW99-0919 OWOW99-0978 OWOW99-1103 OWOW99-1356 OWOW99-1403 OWOW99-1419 OWOW99-1444 OWOW99-1540 OWOW99-0142 OWOW99-0167 OWOW99-0517 OWOW99-0918 OWOW99-1367 OWOW99-0013 OWOW99-0463 OWOW99-0638 OWOW99-1063 OWOW99-1413 OWOW99-1563 OWOW99-0095 OWOW99-0169 Long. DD -102.7355 -103.4950 -100.4095 -102.3409 -98.1846 -96.9633 -101.7811 -102.5531 -98.1129 -101.8481 -95.6776 -101.5539 -96.3740 -71.1640 -71.3410 -71.7674 -71.7401 -71.9652 -74.8627 -74.2645 -74.2684 -74.2473 -75.2780 -74.9980 -103.6595 -107.6105 Lat. DD 42.0064 41.9337 40.9410 42.0069 40.0087 41.4518 42.4001 42.2118 42.7988 41.2503 40.3263 40.4322 41.3094 43.0991 43.6026 43.6595 43.0228 43.3070 39.7848 39.7364 41.1211 40.8269 39.3922 40.9226 35.0939 36.5178 Lake Name L MCCONAUGHY Unnamed Lake Winneoesaukee Newfound Lake Unnamed Loch Lyndon Reservoir Arch Hurly Conservency Lake Mavajo Reservoir St. NE NE NE NE NE NE NE NE NE NE NE NE NE NH NH NH NH NH NJ NJ NJ NJ NJ NJ NM NM County BOX BUTTE SCOTTS BLUFF LINCOLN GARDEN NUCKOLLS COLFAX CHERRY SHERIDAN KNOX KEITH NEMAHA CHASE DOUGLAS ROCKINGHAM BELKNAP GRAFTON HILLSBOROUGH MERRIMACK CAMDEN OCEAN PASSAIC ESSEX CUMBERLAND WARREN QUAY RIOARRIBA Lake Area (ha) 8 784 226 10 7 5 10 7 28 11464 2 652 9 5 18545 1718 8 47 4 18 23 5 3 6 39 1892 Yr. 1 2 2 2 2 3 3 3 4 4 4 4 4 1 1 2 3 4 1 2 2 3 4 4 1 1 Longitude Deg 102 103 100 102 98 96 101 102 98 101 95 101 96 71 71 71 71 71 74 74 74 74 75 74 103 107 Min 44 29 24 20 11 57 46 33 6 50 40 33 22 9 20 46 44 57 51 15 16 14 16 59 39 36 Sec 7.80 42.00 34.20 27.24 4.56 47.88 51.96 11.16 46.44 53.16 39.36 14.04 26.40 50.40 27.60 2.64 24.36 54.72 45.72 52.20 6.24 50.28 40.80 52.80 34.20 37.80 Latitude Deg 42 41 40 42 40 41 42 42 42 41 40 40 41 43 43 43 43 43 39 39 41 40 39 40 35 36 Min 0 56 56 0 0 27 24 12 47 15 19 25 18 5 36 39 1 18 47 44 7 49 23 55 5 31 Sec 23.04 1.32 27.60 24.84 31.32 6.48 0.36 42.48 55.68 1.08 34.68 55.92 33.84 56.76 9.36 34.20 22.08 25.20 5.28 11.04 15.96 36.84 31.92 21.36 38.04 4.08 22 of 35 ------- OWOW99 Site-ID OWOW99-0519 OWOW99-1369 OWOW99-1445 OWOW99-0501 OWOW99-0652 OWOW99-0902 OWOW99-0926 OWOW99-0951 OWOW99-1102 OWOW99-1376 OWOW99-1451 OWOW99-1552 OWOW99-0038 OWOW99-0063 OWOW99-0067 OWOW99-0088 OWOW99-0113 OWOW99-0114 OWOW99-0138 OWOW99-0488 OWOW99-0538 OWOW99-0542 OWOW99-0562 OWOW99-0588 OWOW99-0593 OWOW99-0613 Long. DD -105.0750 -104.3510 -103.9262 -118.3701 -114.3731 -119.5533 -115.4695 -118.3443 -116.0292 -118.3889 -117.1533 -116.2257 -75.5268 -74.7667 -74.5002 -76.9186 -75.4139 -79.3778 -73.5965 -74.1190 -74.5199 -74.4799 -78.8773 -75.2920 -73.7070 -73.7041 Lat. DD 36.8757 32.6128 33.0108 38.5316 36.2826 40.0220 40.1724 38.9805 38.9549 38.8915 41.4146 36.3573 42.5710 41.7675 44.1914 42.6277 44.2527 42.1331 42.1441 42.2356 41.6999 44.2537 42.6172 44.1313 43.7154 41.5026 Lake Name No name playa lake Brantley Reservoir No name plava lake LAKE MEAD Pyramid Lake Ruby Lake Chimney Reservoir Mead Pond Lake Barnabee Tuooer Lake Seneca Lake Sylvia Lake Chatauqua Lake Copake Lake Colgate Lake Unnamed Little Wolf Pond Unnamed Unnamed Brant Lake Southern South Lake St. NM NM NM NV NV NV NV NV NV NV NV NV NY NY NY NY NY NY NY NY NY NY NY NY NY NY County COLFAX CHAVES MINERAL CLARK WASHOE ELKO MINERAL NYE MINERAL HUMBOLDT NYE CHENANGO SULLIVAN FRANKLIN YATES ST LAWRENCE COLUMBIA GREENE SULLIVAN FRANKLIN ERIE LEWIS WARREN PUTNAM Lake Area (ha) 6 8498 1 82 39373 44233 38 84 269 277 881 138 5 2 2584 17413 125 5438 158 11 7 65 2 1 572 4 Yr. 2 4 4 2 2 3 3 3 3 4 4 4 1 1 1 1 1 1 1 2 2 2 2 2 2 2 Longitude Deg 105 104 103 118 114 119 115 118 116 118 117 116 75 74 74 76 75 79 73 74 74 74 78 75 73 73 Min 4 21 55 22 22 33 28 20 1 23 9 13 31 46 30 55 24 22 35 7 31 28 52 17 42 42 Sec 30.00 3.46 34.32 12.36 23.16 11.88 10.20 39.48 45.12 20.04 11.88 32.52 36.48 0.12 0.72 6.96 50.04 40.12 47.40 8.40 11.64 47.64 38.28 31.20 25.20 14.76 Latitude Deg 36 32 33 38 36 40 40 38 38 38 41 36 42 41 44 42 44 42 42 42 41 44 42 44 43 41 Min 52 36 0 31 16 1 10 58 57 53 24 21 34 46 11 37 15 7 8 14 41 15 37 7 42 30 Sec 32.52 46.19 38.88 53.76 57.36 19.20 20.64 49.80 17.64 29.40 52.56 26.28 15.60 3.00 29.04 39.72 9.72 59.20 38.76 8.16 59.64 13.32 1.92 52.68 55.44 9.36 23 of 35 ------- OWOW99 Site-ID OWOW99-0913 OWOW99-1013 OWOW99-1018 OWOW99-1362 OWOW99-1438 OWOW99-1463 OWOW99-1488 OWOW99-1492 OWOW99-1513 OWOW99-1518 OWOW99-1542 OWOW99-0066 OWOW99-0163 OWOW99-0513 OWOW99-0541 OWOW99-0963 OWOW99-1038 OWOW99-1066 OWOW99-1091 OWOW99-1114 OWOW99-1363 OWOW99-1388 OWOW99-1466 OWOW99-1491 OWOW99-1514 OWOW99-0023 Long. DD -75.9898 -74.1704 -73.6822 -77.1845 -76.2675 -72.3292 -73.9587 -74.5445 -74.8449 -73.5952 -73.9226 -82.5221 -81.7622 -82.2338 -83.1276 -83.5746 -81.6191 -84.0402 -84.1174 -83.6802 -81.2526 -81.1403 -84.7258 -82.2990 -80.5713 -96.3983 Lat. DD 43.4336 43.2291 43.3046 43.2367 43.2846 40.9420 41.1617 44.3974 43.8336 43.8205 44.5617 39.1898 40.1524 39.9526 40.6223 41.0512 41.0094 39.8466 39.5665 41.6072 41.3496 40.7884 39.2000 39.7671 41.3069 33.9319 Lake Name Whitney Pond Northville Lake Unnamed Unnamed Pennellville Pond Goldfish Pond DeForest Lake Little Rock Pond Moose Lake Grizzle Ocean Mud Pond Lake Rupert Unnamed Unnamed Unnamed Branch Lake#1 Unnamed Unnamed Unnamed Unnamed Lake Hidden Valley Lake Unnamed Lake Clouse Lake Unnamed Lake St. NY NY NY NY NY NY NY NY NY NY NY OH OH OH OH OH OH OH OH OH OH OH OH OH OH OK County OSWEGO FULTON WARREN WAYNE OSWEGO SUFFOLK ROCKLAND FRANKLIN HERKIMER ESSEX CLINTON VINTON MUSKINGUM LICKING MARION HANCOCK SUMMIT GREENE WARREN LUCAS GEAUGA STARK HAMILTON PERRY TRUMBULL BRYAN Lake Area (ha) 32 8 7 1 7 1 94 7 507 8 45 133 1542 2 2 1 3 9 4 5 2 2 5 13 2 5 Yr. 3 3 3 4 4 4 4 4 4 4 4 1 1 2 2 3 3 3 3 3 4 4 4 4 4 1 Longitude Deg 75 74 73 77 76 72 73 74 74 73 73 82 81 82 83 83 81 84 84 83 81 81 84 82 80 96 Min 59 10 40 11 16 19 57 32 50 35 55 31 45 14 7 34 37 2 7 40 15 8 43 17 34 23 Sec 23.28 13.44 55.92 4.20 3.00 45.12 31.32 40.20 41.64 42.72 21.36 19.56 43.92 1.68 39.36 28.56 8.76 24.72 2.64 48.72 9.36 25.08 32.88 56.40 16.68 53.88 Latitude Deg 43 43 43 43 43 40 41 44 43 43 44 39 40 39 40 41 41 39 39 41 41 40 39 39 41 33 Min 26 13 18 14 17 56 9 23 50 49 33 11 9 57 37 3 0 50 33 36 20 47 12 46 18 55 Sec 0.96 44.76 16.56 12.12 4.56 31.20 42.12 50.64 0.96 13.80 42.12 23.28 8.64 9.36 20.28 4.32 33.84 47.76 59.40 25.92 58.56 18.24 0.00 1.56 24.84 54.84 24 of 35 ------- OWOW99 Site-ID OWOW99-0049 OWOW99-0068 OWOW99-0069 OWOW99-0099 OWOW99-0193 OWOW99-0194 OWOW99-0219 OWOW99-0499 OWOW99-0544 OWOW99-0643 OWOW99-0644 OWOW99-0669 OWOW99-0924 OWOW99-0944 OWOW99-1023 OWOW99-1024 OWOW99-1069 OWOW99-1093 OWOW99-1123 OWOW99-1423 OWOW99-1468 OWOW99-1469 OWOW99-1494 OWOW99-1524 OWOW99-1543 OWOW99-1544 Long. DD -94.8975 -95.5922 -98.4909 -95.4239 -95.3515 -95.8195 -96.3680 -94.6797 -97.5291 -95.3119 -95.2973 -96.7935 -96.2380 -97.9921 -98.0545 -95.1480 -98.6438 -95.1917 -96.5470 -97.6357 -94.9559 -96.5172 -99.3117 -94.8057 -95.6455 -98.1776 Lat. DD 34.9442 36.5821 35.1982 34.0857 35.9778 35.3984 36.2481 34.2803 34.9868 36.0564 35.4847 36.6135 34.5780 35.5221 34.3044 35.2651 35.4976 36.4339 34.4187 34.5867 35.7116 35.1970 34.9257 35.2691 36.5462 36.7337 Lake Name OOLOGAH L Hugo Lake KEYSTONE L BROKEN BOW L TENKILLER FERRY L St. OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK OK County LE FLORE ROGERS CADDO CHOCTAW WAGONER MCINTOSH PAWNEE MCCURTAIN MCCLAIN WAGONER MUSKOGEE OSAGE COAL CANADIAN STEPHENS HASKELL CUSTER MAYES JOHNSTON STEPHENS CHEROKEE SEMINOLE KIOWA LE FLORE ROGERS ALFALFA Lake Area (ha) 1 6100 1654 4950 634 590 5455 5342 12 799 1 2 159 63 2076 6 4 8 41 15 5350 145 1810 1 99 4041 Yr. 1 1 1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 4 4 4 Longitude Deg 94 95 98 95 95 95 96 94 97 95 95 96 96 97 98 95 98 95 96 97 94 96 99 94 95 98 Min 53 35 29 25 21 49 22 40 31 18 17 47 14 59 3 8 38 11 32 38 57 31 18 48 38 10 Sec 51.00 31.92 27.24 26.04 5.40 10.20 4.80 46.92 44.76 42.84 50.28 36.60 16.80 31.56 16.20 52.80 37.68 30.12 49.20 8.52 21.24 1.92 42.12 20.52 43.80 39.36 Latitude Deg 34 36 35 34 35 35 36 34 34 36 35 36 34 35 34 35 35 36 34 34 35 35 34 35 36 36 Min 56 34 11 5 58 23 14 16 59 3 29 36 34 31 18 15 29 26 25 35 42 11 55 16 32 44 Sec 39.12 55.56 53.52 8.52 40.08 54.24 53.16 49.08 12.48 23.04 4.92 48.60 40.80 19.56 15.84 54.36 51.36 2.04 7.32 12.12 41.76 49.20 32.52 8.76 46.32 1.32 25 of 35 ------- OWOW99 Site-ID OWOW99-0076 OWOW99-0101 OWOW99-0451 OWOW99-0576 OWOW99-0629 OWOW99-0901 OWOW99-0929 OWOW99-0976 OWOW99-1001 OWOW99-1101 OWOW99-1353 OWOW99-1401 OWOW99-1454 OWOW99-1501 OWOW99-0039 OWOW99-0089 OWOW99-0188 OWOW99-0213 OWOW99-0489 OWOW99-0663 OWOW99-0988 OWOW99-1014 OWOW99-1088 OWOW99-1113 OWOW99-1417 OWOW99-1517 Long. DD -123.2389 -119.2216 -122.0948 -119.1225 -120.5315 -122.1189 -123.8054 -120.0245 -123.2441 -120.2574 -117.3510 -122.0095 -123.3889 -121.7221 -78.6659 -77.8121 -74.9514 -76.3888 -79.4857 -76.2884 -75.6005 -80.4247 -75.2502 -76.7346 -71.5789 -71.4594 Lat. DD 44.5527 43.4362 42.9494 43.2360 45.7258 44.8230 46.1084 42.1399 43.3729 42.6017 43.4992 42.2938 45.4452 43.6916 41.1581 39.9451 41.2504 41.9443 40.6822 41.2899 40.5611 41.2928 41.4692 40.1593 41.1687 41.7052 Lake Name Crater Lake Harney Lake Lake Umatilla Lake Abert Lake Sabula unnamed pond Pike Lake #3 unnamed pond Crooked Creek Lake Luzerne Lake #6 Lehigh Lake #7 Shenango River Reservoir Wayne-Whitney Lake York Haven Dam Fresh Pond Gorton Pond? St. OR OR OR OR OR OR OR OR OR OR OR OR OR OR PA PA PA PA PA PA PA PA PA PA Rl Rl County LINN HARNEY KLAMATH KLICKITAT MARION CLATSOP LAKE DOUGLAS LAKE MALHEUR KLAMATH WASHINGTON DESCHUTES CLEARFIELD FRANKLIN PIKE BRADFORD ARMSTRONG LUZERNE LEHIGH MERCER WAYNE DAUPHIN WASHINGTON KENT Lake Area (ha) 7 57 5318 9844 11698 26 10 767 6 16397 4577 1498 81 4110 13 2 6 10 151 18 2 1491 46 1596 2526 22 Yr. 1 1 2 2 2 3 3 3 3 3 4 4 4 4 1 1 1 1 2 2 3 3 3 3 4 4 Longitude Deg 123 119 122 119 120 122 123 120 123 120 117 122 123 121 78 77 74 76 79 76 75 80 75 76 71 71 Min 14 13 5 7 31 7 48 1 14 15 21 0 23 43 39 48 57 23 29 17 36 25 15 44 34 27 Sec 20.11 17.87 41.10 21.14 53.54 7.97 19.33 28.20 38.72 26.60 3.67 34.13 19.97 19.67 57.24 43.56 5.04 19.68 8.52 18.24 1.80 28.92 0.72 4.56 44.04 33.84 Latitude Deg 44 43 42 43 45 44 46 42 43 42 43 42 45 43 41 39 41 41 40 41 40 41 41 40 41 41 Min 33 26 56 14 43 49 6 8 22 36 29 17 26 41 9 56 15 56 40 17 33 17 28 9 10 42 Sec 9.54 10.28 57.84 9.67 32.92 22.87 30.06 23.64 22.48 6.08 57.08 37.82 42.61 29.87 29.16 42.36 1.44 39.48 55.92 23.64 39.96 34.08 9.12 33.48 7.32 18.72 26 of 35 ------- OWOW99 Site-ID OWOW99-1567 OWOW99-0012 OWOW99-0112 OWOW99-0211 OWOW99-0212 OWOW99-0462 OWOW99-0536 OWOW99-0662 OWOW99-0936 OWOW99-0937 OWOW99-0987 OWOW99-1061 OWOW99-1087 OWOW99-1412 OWOW99-1486 OWOW99-1537 OWOW99-1562 OWOW99-0007 OWOW99-0056 OWOW99-0107 OWOW99-0203 OWOW99-0206 OWOW99-0457 OWOW99-0531 OWOW99-0582 OWOW99-0628 Long. DD -71.4065 -80.1384 -79.1741 -80.9912 -80.9015 -80.1292 -82.5805 -80.7032 -81.3848 -80.2755 -81.4667 -81.6083 -81.6246 -79.2482 -83.1017 -81.9264 -80.8089 -98.0560 -102.2547 -97.1801 -101.3716 -99.8458 -96.4624 -103.2273 -97.5077 -103.8945 Lat. DD 41.9839 34.5133 34.3024 33.2641 33.6414 34.3049 34.6606 34.3094 33.6076 33.5042 34.0877 33.8239 34.8750 33.3649 34.5784 34.8039 34.4193 43.7564 45.7699 44.8678 44.1562 45.7071 44.4955 45.1155 45.6993 44.2735 Lake Name Arnolds Mills Reservoir L MURRAY HARTWELL RES Lake Wateree St. Rl SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SC SD SD SD SD SD SD SD SD SD County PROVIDENCE CHESTERFIELD DILLON BAMBERG CALHOUN DARLINGTON ANDERSON KERSHAW AIKEN CLARENDON NEWBERRY AIKEN CHEROKEE GEORGETOWN OCONEE SPARTANBURG KERSHAW DAVISON PERKINS CODINGTON HAAKON CAMPBELL BROOKINGS BUTTE MARSHALL LAWRENCE Lake Area (ha) 6 3 159 37 18 36 3 2 18 3 19602 6 6 4 6881 3 5548 284 959 1124 5 6 616 4 83 681 Yr. 4 1 1 1 1 2 2 2 3 3 3 3 3 4 4 4 4 1 1 1 1 1 2 2 2 2 Longitude Deg 71 80 79 80 80 80 82 80 81 80 81 81 81 79 83 81 80 98 102 97 101 99 96 103 97 103 Min 24 8 10 59 54 7 34 42 23 16 28 36 37 14 6 55 48 3 15 10 22 50 27 13 30 53 Sec 23.40 18.24 26.76 28.32 5.40 45.12 49.80 11.52 5.28 31.80 0.12 29.88 28.56 53.52 6.12 35.04 32.04 21.60 16.92 48.36 17.76 44.88 44.64 38.28 27.72 40.20 Latitude Deg 41 34 34 33 33 34 34 34 33 33 34 33 34 33 34 34 34 43 45 44 44 45 44 45 45 44 Min 59 30 18 15 38 18 39 18 36 30 5 49 52 21 34 48 25 45 46 52 9 42 29 6 41 16 Sec 2.04 47.88 8.64 50.76 29.04 17.64 38.16 33.84 27.36 15.12 15.72 26.04 30.00 53.64 42.24 14.04 9.48 23.04 11.64 4.08 22.32 25.56 43.80 55.80 57.48 24.60 27 of 35 ------- OWOW99 Site-ID OWOW99-0631 OWOW99-0653 OWOW99-0657 OWOW99-0928 OWOW99-0931 OWOW99-0982 OWOW99-1007 OWOW99-1031 OWOW99-1056 OWOW99-1107 OWOW99-1407 OWOW99-1453 OWOW99-1481 OWOW99-1507 OWOW99-1553 OWOW99-1556 OWOW99-1557 OWOW99-0087 OWOW99-0187 OWOW99-0487 OWOW99-0561 OWOW99-0587 OWOW99-0597 OWOW99-1036 OWOW99-1086 OWOW99-1487 Long. DD -103.4072 -100.0315 -99.3955 -103.4528 -99.1439 -101.0124 -97.2311 -98.2920 -100.5331 -97.5925 -97.1505 -103.7237 -102.1920 -97.4514 -103.4179 -97.2857 -99.7300 -86.5603 -83.8330 -85.2748 -88.4150 -86.7644 -89.3677 -88.0792 -84.7785 -83.3651 Lat. DD 45.8542 43.3437 44.6185 44.8720 45.9093 44.3660 45.1795 43.4149 44.8741 44.4791 44.1469 45.0254 45.2425 45.3803 43.3078 45.7157 43.9121 36.0991 36.3113 36.5651 35.5581 36.4128 35.2595 36.4316 35.6188 35.9973 Lake Name OAHE RES J PERCY PRIEST L Morris Lake Dale Hollow Lake KENTUCKY L DOUGLAS L St. SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD SD TN TN TN TN TN TN TN TN TN County HARDING TRIPP HYDE BUTTE MCPHERSON STANLEY DAY DOUGLAS DEWEY KINGSBURY LAKE BUTTE PERKINS DAY FALL RIVER MARSHALL LYMAN DAVIDSON UNION CLAY HENDERSON ROBERTSON FAYETTE HENRY MEIGS JEFFERSON Lake Area (ha) 9 3 3 5 129 25 83 38 61520 119 95 7 43 940 1742 54 3 5370 3749 10726 184 5 5 46342 2 11139 Yr. 2 2 2 3 3 3 3 3 3 3 4 4 4 4 4 4 4 1 1 2 2 2 2 3 3 4 Longitude Deg 103 100 99 103 99 101 97 98 100 97 97 103 102 97 103 97 99 86 83 85 88 86 89 88 84 83 Min 24 1 23 27 8 0 13 17 31 35 9 43 11 27 25 17 43 33 49 16 24 45 22 4 46 21 Sec 25.92 53.40 43.80 10.08 38.04 44.64 51.96 31.20 59.16 33.00 1.80 25.32 31.20 5.04 4.44 8.52 48.00 37.08 58.80 29.28 54.00 51.84 3.72 45.12 42.60 54.36 Latitude Deg 45 43 44 44 45 44 45 43 44 44 44 45 45 45 43 45 43 36 36 36 35 36 35 36 35 35 Min 51 20 37 52 54 21 10 24 52 28 8 1 14 22 18 42 54 5 18 33 33 24 15 25 37 59 Sec 15.12 37.32 6.60 19.20 33.48 57.60 46.20 53.64 26.76 44.76 48.84 31.44 33.00 49.08 28.08 56.52 43.56 56.76 40.68 54.36 29.16 46.08 34.20 53.76 7.68 50.28 28 of 35 ------- OWOW99 Site-ID OWOW99-1512 OWOW99-1536 OWOW99-0020 OWOW99-0021 OWOW99-0046 OWOW99-0048 OWOW99-0070 OWOW99-0073 OWOW99-0096 OWOW99-0120 OWOW99-0121 OWOW99-0123 OWOW99-0145 OWOW99-0148 OWOW99-0170 OWOW99-0195 OWOW99-0196 OWOW99-0198 OWOW99-0220 OWOW99-0221 OWOW99-0223 OWOW99-0470 OWOW99-0471 OWOW99-0473 OWOW99-0495 OWOW99-0496 Long. DD -86.4277 -84.2180 -95.3874 -100.5777 -97.4428 -98.3790 -98.0258 -94.6013 -97.8593 -97.3485 -99.2766 -97.1985 -96.2522 -94.7224 -100.9665 -94.9041 -99.6494 -95.6121 -95.6834 -97.9284 -96.0107 -98.1155 -99.5140 -96.7899 -100.3492 -96.8272 Lat. DD 36.0389 35.6086 30.1851 31.9371 31.9740 33.7103 30.4154 32.8300 32.0622 26.2842 33.9259 33.0425 29.4629 33.2888 32.4319 29.8449 28.9065 33.4843 31.1694 28.2013 32.9492 26.1452 32.0370 33.8561 34.4613 32.0145 Lake Name Tellico Lake Roaers Lake E V Spence Reservoir Lake Arrowhead Lake Travis Flaa Branch Lake Unnamed lake Santa Rosa Lake Bullock Lake Lake Corpus Christ! Lake Tawakoni Lake Coleman L TEXOMA Lake Childress Unnamed lake St. TN TN TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX County RUTHERFORD MONROE MONTGOMERY COKE BOSQUE CLAY TRAVIS MARION BOSQUE CAMERON WILBARGER DENTON WHARTON MORRIS MITCHELL CHAMBERS ZAVALA LAMAR HOUSTON LIVE OAK HUNT HIDALGO COLEMAN GRAYSON CHILDRESS NAVARRO Lake Area (ha) 714 6639 9 6055 2208 6561 7240 557 11 35 660 380 2 1239 2 4 5 6 23 7831 15333 8 705 23549 121 12 Yr. 4 4 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 Longitude Deg 86 84 95 100 97 98 98 94 97 97 99 97 96 94 100 94 99 95 95 97 96 98 99 96 100 96 Min 25 13 23 34 26 22 1 36 51 20 16 11 15 43 57 54 38 36 41 55 0 6 30 47 20 49 Sec 39.72 4.80 14.64 39.72 34.08 44.40 32.88 4.68 33.48 54.60 35.76 54.60 7.92 20.64 59.40 14.76 57.84 43.56 0.24 42.24 38.52 55.80 50.40 23.64 57.12 37.92 Latitude Deg 36 35 30 31 31 33 30 32 32 26 33 33 29 33 32 29 28 33 31 28 32 26 32 33 34 32 Min 2 36 11 56 58 42 24 49 3 17 55 2 27 17 25 50 54 29 10 12 56 8 2 51 27 0 Sec 20.04 30.96 6.36 13.56 26.40 37.08 55.44 48.00 43.92 3.12 33.24 33.00 46.44 19.68 54.84 41.64 23.40 3.48 9.84 4.68 57.12 42.72 13.20 21.96 40.68 52.20 29 of 35 ------- OWOW99 Site-ID OWOW99-0520 OWOW99-0524 OWOW99-0545 OWOW99-0548 OWOW99-0570 OWOW99-0573 OWOW99-0596 OWOW99-0598 OWOW99-0620 OWOW99-0645 OWOW99-0646 OWOW99-0648 OWOW99-0670 OWOW99-0671 OWOW99-0673 OWOW99-0920 OWOW99-0921 OWOW99-0945 OWOW99-0946 OWOW99-0948 OWOW99-0970 OWOW99-0973 OWOW99-0974 OWOW99-0995 OWOW99-0996 OWOW99-0998 Long. DD -96.0261 -94.1918 -95.0674 -94.3946 -98.2822 -95.5984 -99.0068 -96.6693 -98.3474 -97.6087 -98.4200 -96.6480 -94.8510 -97.8103 -95.4881 -101.8108 -97.5738 -95.9137 -99.1748 -96.5444 -96.6798 -94.3321 -94.1659 -96.2954 -101.1158 -96.0390 Lat. DD 28.7684 30.8490 29.5604 32.2129 32.6120 33.8270 32.7753 33.3078 29.8943 31.0062 28.3815 32.6450 30.4974 28.1089 32.1860 35.4638 31.1665 31.2285 27.9740 33.1304 28.3294 33.2844 32.0277 30.1495 30.1436 32.0818 Lake Name B A Steinhaqen Lake Clear Lake Shelby Lake Lake Pat Mayse HUBBARDCRRES ASCS Lake Riser 638 Stillhouse Hollow Lake L PALESTINE Lake Belton Unnamed lake Lake Lavon Wriqht Patman Lake Sabine River overflow St. TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX County MATAGORDA HARRIS PANOLA PALO PINTO LAMAR STEPHENS COLLIN COMAL BELL MCMULLEN DALLAS POLK SAN PATRICIO HENDERSON POTTER BELL LEON WEBB COLLIN CALHOUN BOWIE PANOLA WASHINGTON VAL VERDE HENDERSON Lake Area (ha) 18 5549 812 5 7 2390 5960 7 19 2664 6 6 6 7 9533 655 1052 2 20 81 2 11360 5 2 51 10 Yr. 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 Longitude Deg 96 94 95 94 98 95 99 96 98 97 98 96 94 97 95 101 97 95 99 96 96 94 94 96 101 96 Min 1 11 4 23 16 35 0 40 20 36 25 38 51 48 29 48 34 54 10 32 40 19 9 17 6 2 Sec 33.96 30.59 2.64 40.56 55.92 54.24 24.48 9.48 50.64 31.32 12.00 52.80 3.60 37.08 17.16 38.88 25.68 49.32 29.28 39.84 47.28 55.56 57.24 43.44 56.88 20.40 Latitude Deg 28 30 29 32 32 33 32 33 29 31 28 32 30 28 32 35 31 31 27 33 28 33 32 30 30 32 Min 46 50 33 12 36 49 46 18 53 0 22 38 29 6 11 27 9 13 58 7 19 17 1 8 8 4 Sec 6.24 56.29 37.44 46.44 43.20 37.20 31.08 28.08 39.48 22.32 53.40 42.00 50.64 32.04 9.60 49.68 59.40 42.60 26.40 49.44 45.84 3.84 39.72 58.20 36.96 54.48 30 of 35 ------- OWOW99 Site-ID OWOW99-1021 OWOW99-1045 OWOW99-1049 OWOW99-1070 OWOW99-1071 OWOW99-1073 OWOW99-1094 OWOW99-1095 OWOW99-1098 OWOW99-1120 OWOW99-1121 OWOW99-1370 OWOW99-1373 OWOW99-1395 OWOW99-1399 OWOW99-1420 OWOW99-1421 OWOW99-1446 OWOW99-1470 OWOW99-1471 OWOW99-1473 OWOW99-1495 OWOW99-1496 OWOW99-1498 OWOW99-1521 OWOW99-1523 Long. DD -98.6771 -98.5051 -94.5522 -95.3163 -100.9560 -95.5320 -101.6812 -98.0244 -95.5161 -97.8470 -98.9015 -96.8215 -94.1256 -98.0162 -93.7712 -99.2401 -99.2335 -96.2172 -96.5598 -98.6921 -96.9868 -95.5053 -100.1961 -96.3628 -100.3713 -97.6110 Lat. DD 33.3955 32.0189 31.5542 28.9617 29.6535 33.1012 35.6553 30.3621 32.5682 27.1988 33.7473 32.2412 32.7491 28.9365 31.5230 26.8182 31.3159 31.9798 30.7472 28.6395 33.1494 29.6160 28.5733 33.1896 33.0911 33.4887 Lake Name Lake Proctor CADDO L Toledo Bend Reservoir Richland Reservoir Lake Lewisville What Is It Pond St. TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX TX County YOUNG COMANCHE NACOGDOCHES BRAZORIA VAL VERDE HOPKINS MOORE TRAVIS SMITH KENEDY ARCHER ELLIS MARION KARNES SABINE ZAPATA MCCULLOCH ROBERTSON ATASCOSA DENTON FORT BEND MAVERICK COLLIN STONEWALL MONTAGUE Lake Area (ha) 9 1913 3 2 588 5 593 225 6 5 3 9 10794 8 67141 116 6 18124 2 1 8590 1 3 9 7 5 Yr. 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Longitude Deg 98 98 94 95 100 95 101 98 95 97 98 96 94 98 93 99 99 96 96 98 96 95 100 96 100 97 Min 40 30 33 18 57 31 40 1 30 50 54 49 7 0 46 14 14 13 33 41 59 30 11 21 22 36 Sec 37.56 18.36 7.92 58.68 21.60 55.20 52.32 27.84 57.96 49.20 5.40 17.40 32.16 58.32 16.32 24.36 0.60 1.92 35.28 31.56 12.48 19.08 45.96 46.08 16.68 39.60 Latitude Deg 33 32 31 28 29 33 35 30 32 27 33 32 32 28 31 26 31 31 30 28 33 29 28 33 33 33 Min 23 1 33 57 39 6 39 21 34 11 44 14 44 56 31 49 18 58 44 38 8 36 34 11 5 29 Sec 43.80 8.04 15.12 42.12 12.60 4.32 19.08 43.56 5.52 55.68 50.28 28.32 56.76 11.40 22.80 5.52 57.24 47.14 49.92 22.20 57.84 57.60 23.88 22.56 27.96 19.32 31 of 35 ------- OWOW99 Site-ID OWOW99-1545 OWOW99-1570 OWOW99-1571 OWOW99-1573 OWOW99-0102 OWOW99-0144 OWOW99-0152 OWOW99-0526 OWOW99-0626 OWOW99-0927 OWOW99-1027 OWOW99-1051 OWOW99-1352 OWOW99-1476 OWOW99-0064 OWOW99-0090 OWOW99-0512 OWOW99-0514 OWOW99-0614 OWOW99-0914 OWOW99-0964 OWOW99-1039 OWOW99-1089 OWOW99-1364 OWOW99-1462 OWOW99-1539 Long. DD -97.3344 -95.5857 -99.3188 -95.5930 -113.7755 -110.8572 -110.2791 -111.8377 -112.6432 -111.8883 -110.1109 -111.1449 -110.1033 -111.8073 -77.8438 -77.3122 -79.0903 -78.5318 -77.3104 -77.7039 -76.5704 -75.9664 -78.9541 -76.3460 -79.4531 -77.6024 Lat. DD 30.0141 30.4678 26.9216 32.6230 37.2618 37.3143 40.6966 39.0707 40.8812 41.8315 40.7634 40.1871 40.3998 40.2025 38.0644 37.9672 36.6820 36.5703 37.4233 38.8207 36.8671 37.4214 36.7873 37.0230 36.7772 37.2620 Lake Name Lake Conroe INTERNATIONAL FALCON RES Strawberry Reservoir Utah Lake Lake Anna unnamed Big Lake Griggs Pond unnamed Lone Star Lake Banister Lake unnamed pond Lake Chesdin St. TX TX TX TX UT UT UT UT UT UT UT UT UT UT VA VA VA VA VA VA VA VA VA VA VA VA County BASTROP MONTGOMERY ZAPATA SMITH WASHINGTON KANE DUCHESNE SAN PETE TOOELE CACHE DUCHESNE WASATCH DUCHESNE LOUISA CAROLINE HALIFAX MECKLENBURG HENRICO PRINCE WILLIAM SUFFOLK NORTHAMPTON HALIFAX HAMPTON PITTSYLVANIA CHESTERFIELD Lake Area (ha) 4 8030 15802 3 101 912 4 15 921 7 10 3172 2 0 5254 11 10 10 6 3 13 628 154 170 1 1316 Yr. 4 4 4 4 1 1 1 2 2 3 3 3 4 4 1 1 2 2 2 3 3 3 3 4 4 4 Longitude Deg 97 95 99 95 113 110 110 111 112 111 110 111 110 111 77 77 79 78 77 77 76 75 78 76 79 77 Min 20 35 19 35 46 51 16 50 38 53 6 8 6 48 50 18 5 31 18 42 34 57 57 20 27 36 Sec 3.84 8.52 7.68 34.80 31.80 25.92 44.76 15.72 35.52 17.88 39.24 41.64 11.88 26.21 37.68 43.92 25.08 54.48 37.44 14.04 13.44 59.04 14.76 45.60 11.16 8.64 Latitude Deg 30 30 26 32 37 37 40 39 40 41 40 40 40 40 38 37 36 36 37 38 36 37 36 37 36 37 Min 0 28 55 37 15 18 41 4 52 49 45 11 23 12 3 58 40 34 25 49 52 25 47 1 46 15 Sec 50.76 4.08 17.76 22.80 42.48 51.48 47.76 14.52 52.32 53.40 48.24 13.56 59.28 8.86 51.84 1.92 55.20 13.08 23.88 14.52 1.56 17.04 14.28 22.80 37.92 43.20 32 of 35 ------- OWOW99 Site-ID OWOW99-0093 OWOW99-0942 OWOW99-1042 OWOW99-1092 OWOW99-1467 OWOW99-0004 OWOW99-0080 OWOW99-0154 OWOW99-0179 OWOW99-0202 OWOW99-0204 OWOW99-0504 OWOW99-0529 OWOW99-0654 OWOW99-0954 OWOW99-0979 OWOW99-1054 OWOW99-1354 OWOW99-1379 OWOW99-1479 OWOW99-1554 OWOW99-0008 OWOW99-0058 OWOW99-0059 OWOW99-0108 OWOW99-0133 Long. DD -72.8915 -72.0592 -72.4259 -73.2782 -72.3081 -121.3595 -117.5813 -120.9586 -119.5883 -123.7674 -122.8397 -120.3321 -121.1618 -121.3833 -119.4764 -122.4597 -119.3222 -117.2925 -118.8874 -118.9817 -121.6659 -89.2323 -92.2221 -89.0859 -92.6510 -90.9843 Lat. DD 42.8282 44.7480 43.6486 44.8277 44.8707 47.3342 47.7677 48.5632 46.9819 48.0848 46.9928 48.0261 46.6403 47.5843 48.9145 48.0215 46.9868 48.4300 48.0631 46.0048 47.6052 44.0659 45.7970 46.0610 45.2943 45.8117 Lake Name Lake Whitingham Lake Willouqhby Lake Pinneo Kidder Pond Keechelus Lake Dry Lake Unnamed/Vulcan Frenchman Hills Lake Cresent Lake unnamed Lake Chelan Rimrock Lake Lake Dorothy Mud Lake Lone Lake Potholes Reservoir Buffalo Lake LakeWallula Calligan Lake Iroqami (Fish) Lake Warner Lake Noseeum Lake Osceola Lake Lake Winter St. vr vr vr vr vr WA WA WA WA WA WA WA WA WA WA WA WA WA WA WA WA Wl Wl Wl Wl Wl County WINDHAM ORLEANS WINDSOR GRAND ISLE ORLEANS KITTITAS SPOKANE SKAGIT GRANT CLALLAM THURSTON CHELAN YAKIMA KING OKANOGAN ISLAND GRANT PEND OREILLE OKANOGAN BENTON KING WAUSHARA BURNETT VILAS POLK SAWYER Lake Area (ha) 1565 670 7 3406 6 955 5 3 138 1995 6 13091 952 102 4 34 11333 936 226 12961 117 116 71 5 17 110 Yr. 1 3 3 3 4 1 1 1 1 1 1 2 2 2 3 3 3 4 4 4 4 1 1 1 1 1 Longitude Deg 72 72 72 73 72 121 117 120 119 123 122 120 121 121 119 122 119 117 118 118 121 89 92 89 92 90 Min 53 3 25 16 18 21 34 57 35 46 50 19 9 22 28 27 19 17 53 58 39 13 13 5 39 59 Sec 29.40 33.12 33.24 41.52 29.16 34.06 52.61 30.82 17.77 2.71 22.88 55.38 42.44 59.88 34.97 34.81 19.99 33.07 14.50 54.16 57.17 56.28 19.56 9.24 3.60 3.48 Latitude Deg 42 44 43 44 44 47 47 48 46 48 46 48 46 47 48 48 46 48 48 46 47 44 45 46 45 45 Min 49 44 38 49 52 20 46 33 58 5 59 1 38 35 54 1 59 25 3 0 36 3 47 3 17 48 Sec 41.52 52.80 54.96 39.72 14.52 2.94 3.58 47.63 54.88 5.32 34.19 33.96 25.08 3.41 52.24 17.47 12.48 48.00 47.02 17.21 18.54 57.24 49.20 39.60 39.48 42.12 33 of 35 ------- OWOW99 Site-ID OWOW99-0134 OWOW99-0158 OWOW99-0208 OWOW99-0458 OWOW99-0508 OWOW99-0533 OWOW99-0565 OWOW99-0608 OWOW99-0658 OWOW99-0666 OWOW99-0958 OWOW99-0959 OWOW99-0983 OWOW99-0991 OWOW99-1033 OWOW99-1058 OWOW99-1059 OWOW99-1083 OWOW99-1084 OWOW99-1108 OWOW99-1365 OWOW99-1383 OWOW99-1408 OWOW99-1433 Long. DD -89.5894 -90.5273 -89.6991 -89.9860 -89.5351 -91.5771 -91.1565 -90.1691 -89.6562 -88.4157 -91.3394 -89.3112 -89.1146 -89.0375 -91.2826 -91.8659 -89.1653 -92.3092 -89.5527 -91.3828 -90.8791 -89.8021 -90.4251 -92.6653 Lat. DD 45.8619 46.2912 44.8103 43.9352 45.6967 45.7591 43.3500 46.0857 45.2854 44.0020 46.1505 45.8418 44.5307 43.9647 46.3544 45.4135 45.5817 45.6438 46.1376 45.2325 43.1321 45.9586 44.1284 45.4906 Lake Name Sweeney Lake Maki Lake L DUBAY/Big Eau Pleine Res Castle Lake Bob's Lake unnamed Turtle Flambeau Flowage Black Alder Lake Lake Winnebago Pacwawong Lake Boa Lake Hatch Lake unnamed Flynn Lake Yellow River Reservoir Mars (Seguilla) Lake Crooked Lake Big Gibson Lake Calkins North Lake unnamed Bolton Lake Unnamed I Beede Lake St. Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl Wl County ONEIDA IRON MARATHON JUNEAU ONEIDA WASHBURN CRAWFORD LINCOLN WINNEBAGO SAWYER ONEIDA WAUPACA GREEN LAKE BAYFIELD BARRON ONEIDA POLK VILAS CHIPPEWA CRAWFORD VILAS MONROE POLK Lake Area (ha) 78 17 5356 5010 8 4 59 0 5 53757 76 20 46 7 27 21 16 8 48 5 5 57 6 2 Yr. 1 1 1 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4 Longitude Deg 89 90 89 89 89 91 91 90 89 88 91 89 89 89 91 91 89 92 89 91 90 89 90 92 Min 35 31 41 59 32 34 9 10 39 24 20 18 6 2 16 51 9 18 33 22 52 48 25 39 Sec 21.84 38.28 56.76 9.60 6.36 37.56 23.40 8.72 22.32 56.52 21.84 40.32 52.56 15.00 57.36 57.24 55.08 33.12 9.72 58.08 44.76 7.56 30.36 55.08 Latitude Deg 45 46 44 43 45 45 43 46 45 44 46 45 44 43 46 45 45 45 46 45 43 45 44 45 Min 51 17 48 56 41 45 21 5 17 0 9 50 31 57 21 24 34 38 8 13 7 57 7 29 Sec 42.84 28.32 37.08 6.72 48.12 32.76 0.00 8.52 7.44 7.20 1.80 30.48 50.52 52.92 15.84 48.60 54.12 37.68 15.36 57.00 55.56 30.96 42.24 26.16 34 of 35 ------- OWOW99 Site-ID OWOW99-1483 OWOW99-1484 OWOW99-1533 OWOW99-1558 OWOW99-1566 OWOW99-0637 OWOW99-0052 OWOW99-0502 OWOW99-0527 OWOW99-0528 OWOW99-0602 OWOW99-1052 OWOW99-1053 OWOW99-1077 OWOW99-1078 OWOW99-1377 OWOW99-1477 OWOW99-1478 OWOW99-1503 OWOW99-1527 Long. DD -92.2409 -88.2877 -91.2291 -88.8840 -88.3072 -80.8542 -109.3330 -106.9291 -109.3050 -109.2586 -110.6277 -110.3633 -106.2468 -109.6685 -110.3662 -106.2790 -109.3615 -106.7534 -106.1244 -110.8467 Lat. DD 45.9427 45.2655 45.5345 45.1419 43.0729 38.2409 43.0084 42.6746 42.8726 44.4925 44.2998 43.4642 43.1947 43.3312 44.4549 41.3601 42.8049 44.4843 42.7497 43.8054 Lake Name Upper Loon Lake (Phernetton) Mirror Lake McGee Lake Pewaukee Lake Summersville Lake Yellowstone Lake St. Wl Wl Wl Wl Wl wv WY WY WY WY WY WY WY WY WY WY WY WY WY WY County BURNETT MARINETTE RUSK LANGLADE WAUKESHA NICHOLAS FREMONT NATRONA FREMONT PARK TETON TETON NATRONA FREMONT ALBANY SUBLETTE JOHNSON NATRONA TETON Lake Area (ha) 24 4 1617 8 985 844 4 1 73 1385 1116 35 24 40 0 5 3 821 8 4 Yr. 4 4 4 4 4 2 1 2 2 2 2 3 3 3 3 4 4 4 4 4 Longitude Deg 92 88 91 88 88 80 109 106 109 109 110 110 106 109 110 106 109 106 106 110 Min 14 17 13 53 18 51 19 55 18 15 37 21 14 40 21 16 21 45 7 50 Sec 27.24 15.72 44.76 2.40 25.92 15.12 58.80 44.76 18.00 30.96 39.72 47.88 48.48 6.60 58.43 44.40 41.40 12.24 27.84 48.12 Latitude Deg 45 45 45 45 43 38 43 42 42 44 44 43 43 43 44 41 42 44 42 43 Min 56 15 32 8 4 14 0 40 52 29 17 27 11 19 27 21 48 29 44 48 Sec 33.72 55.80 4.20 30.84 22.44 27.24 30.24 28.56 21.36 33.00 59.28 51.12 40.92 52.32 17.53 36.36 17.64 3.48 58.92 19.44 35 of 35 ------- ------- Appendix B Standard Operating Procedure: Fish Tissue Sample Collection Procedures for a National Study of Chemical Residues in Lake Fish Tissue ------- ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 1 of 9 Standard Operating Procedure 1444444444444444444444444444444444 Fish Tissue Sample Collection Procedures for a National Study of Chemical Residues in Lake Fish Tissue 444444444444444444444444444444444444444444444444444444444444444444444444444444 Scope and Applicability: This Standard Operating Procedure (SOP) must be followed by all Field Sample Collection Teams involved with the USEPA Office of Water's National Study of Chemical Residues in Lake Fish Tissue. Adherence to the SOP will ensure that field sampling activities will be performed the same way every time, i.e., are standardized, for all sampling participants. Fish tissue sample collection procedures are presented below as sequential steps, and include specific equipment, materials, and methods required to perform field sampling activities only. Responsibility and Personnel Qualifications: This procedure may be used by any Field Sampling Teams that have been authorized by the USEPA Project Manager or the USEPA Regional/State/ Tribal Fish Sampling Coordinators to collect fish for the National Study of Chemical Residues in Lake Fish Tissue. References: U.S. Environmental Protection Agency (USEPA). 1995. Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories. Volume 1: Fish Sampling and Analysis. Second Edition. U.S. Environmental Protection Agency, Office of Water, Washington, D.C. EPA 823-R-95-007. U.S. Environmental Protection Agency (USEPA). 1997. Quality Assurance Project Plan for the Cook Inlet Contaminant Study Sampling. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, D.C. U.S. Environmental Protection Agency (USEPA). 1997. Sampling Plan for Conducting Field Sampling and Chemical Analysis for the Cook Inlet Contaminant Study. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, D.C. U.S. Environmental Protection Agency (USEPA). 1999a. EPA Requirements for Quality Assurance Project Plans. U.S. Environmental Protection Agency, Quality Assurance Division, Washington, D.C. Interim Final. EPA QA/R-5. U.S. Environmental Protection Agency (USEPA). 1999b. National Study of Chemical Residues in Lake Fish Tissue: Study Design. U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology, Washington, D.C. ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 2 of 9 Precautions: Follow usual safety precautions for working in the field. Boats and/or electrofishing equipment should only be operated by qualified, experienced operators trained in their proper use. Each vessel must be equipped with the appropriate Coast Guard-required safety equipment (including personal floatation devices for each field team member). If electrofishing equipment is used for sample collection, each team member must be insulated from the water, boat, and electrodes via rubber boots and gloves. Equipment/Materials: Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment)(a) Electrofishing equipment - OPTIONAL (including variable voltage pulsator unit, generator, electrodes, wiring cables, dip nets, protective gloves, protective boots, and all necessary safety equipment)(a) Nets - OPTIONAL (including trawls, seines, gill nets, fyke nets, trammel nets, hoop nets, pound nets, trap nets)(a) Angling equipment - OPTIONAL (including fishing rods, reels, line, terminal tackle, trot lines/3-1 Coast Guard-approved personal floatation devices Maps of target lakes and access routes Global Positioning System (GPS) unit - OPTIONAL (a) pH meter (including associated calibration supplies)(a) Livewell and/or buckets Measuring board (millimeter scale) Ice chests(b) Aluminum foil (solvent-rinsed and baked)(b) Heavy-duty food grade polyethylene tubing(b) Large plastic (composite) bags(b) Knife or scissors Clean nitrile gloves(b) Field Record Forms(b) Sample Identification Labels(b) Chain-of-Custody Forms(b) Chain-of-Custody Labels(b) Scientific collection permit Dry Ice(b) Black ballpoint pens and/or waterproof markers Clipboard Packing/strapping tape ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 3 of 9 Overnight courier airbills(b) Plastic cable ties(b) Plastic bubble-wrap(b) First aid kit and emergency telephone numbers (a) Selection and exact specifications at the discretion of the experienced on-site fisheries biologist. Provided by the sample control center. Procedures: 1. Identify the target lake to be sampled using the USEPA Office of Water's Target Lake List. Locate the target lake via the coordinates provided in the Target Lake List and USGS topographic maps (or equivalent maps). 2. Based on site reconnaissance, determine whether the target lake meets the definition of a suitable lake for sampling for the purposes of this study, i.e., each lake must: be a permanent body of water of at least one hectare in surface area, have a minimum of 1,000 m2 of open (unvegetated) water, be greater than 1 meter deep, and have a permanent fish population (e.g., no annual winterkill, not recently stocked with young fish). If the target lake meets all of the above criteria, and if in the case of private property, the landowner allows access/permission to sample the lake, proceed with Step 3. If the lake does not meet the definition of a lake and/or if a private landowner denies access, record the problem and contact the USEPA Project Manager and/or the Terra Tech Task Leader. 3. Assemble an array of both active and passive gear types, to ensure the collection of the desired target numbers and species of fish. Selection of the most appropriate gear type(s) for a particular target lake will be at the discretion of the experienced on-site fisheries biologist. Detailed procedures for use or deployment of all possible gear types are not included here. However, if passive collection devices (e.g., gill nets) are used, they must be checked frequently (e.g., several times daily if possible, but at least every 24 hours) to ensure a limited time lag between fish entrapment and sample preparation. Sampling Teams must be qualified, experienced, and/or trained on the safe and effective use of each gear type selected. 4. Sampling gear will be selected and deployed to obtain samples of both predator species and bottom-dwelling species. Suggested target species, listed in order of preference, are as follows: ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 4 of 9 11 If I Ł 1 2 E I Ł 1 S Ł Family name Centrarchidae Percidae Percichthyidae Esocidae Salmonidae Cyprinidae Ictaluridae Catostomidae Common name Largemouth bass Smallmouth bass Black crappie White crappie Walleye Yellow perch White bass Northern pike Lake trout Brown trout Rainbow trout Brook trout Common carp Channel catfish Blue catfish Brown bullhead Yellow bullhead White sucker Scientific name Micropterus salmoides Micropterus dolomieu Pomoxis nigromaculatus Pomoxis annularis Stizostedion vitreum Percaflavescens Morone chrysops Esox lucius Salvelinus namaycush Salmo trutta Oncorhynchus mykiss Salvelinus fontinalis Cyprinus carpio Ictalurus punctatus Ictalurus furcatus Ameiurus nebulosus Ameiurus natalis Catostomus commersoni 5. As soon as fish have been obtained via active collection methods (or removed from passive collection devices) they must be identified to species. Clean nitrile gloves must be worn during the sample handling process. Potential target species/individuals will be rinsed in ambient water to remove any foreign material from the external surface and placed in clean holding containers (e.g., livewells, buckets). Nontarget fishes or small specimens are returned to the lake. 6. One predator and one bottom-dwelling species composite will be retained from each target lake. Each composite must consist of five fish of adequate size (i.e., adult specimens that collectively will provide greater than 560 grams of edible tissue for predators and 560 grams of total body tissue for bottom dwellers) for analysis. Select fish for each composite based on the following criteria: all are of the same species, all satisfy legal requirements of harvestable size (or weight), or at least be of consumable size if no legal harvest requirements are in effect, all are of similar size, so that the smallest individual in a composite is no less than 75% of the total length of the largest individual, and ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 5 of 9 all are collected at the same time, i.e., collected as close to the same time as possible, but no more than one week apart (Note: Individual fish may have to be frozen until all fish to be included in the composite are available for delivery to the sample preparation laboratory). Accurate taxonomic identification is essential in assuring and defining the organisms that have been composited and submitted for analysis. Under no circumstances should individuals from different species be used in a single composite sample. 7. Following selection of five fish for each of the two composites that meet the above-listed criteria for compositing, measure each to determine total body length. Measure total length of each specimen in millimeters, from the anterior-most part of the fish to the tip of the longest caudal finray (when the lobes of the caudal fin are depressed dorsoventrally). 8. Record species retained, specimen length, location collected and sampling date and time on the Field Record Form (Figure 1) in black ink. Complete site location description portions of the form, and draw a simple sketch of the sampling area in the space provided. One Field Record Form will be completed for each composite collected from the target lake. 9. Assign the unique ten-character composite sample ID number to each composite as directed on the Field Record Form (Figure 1): state of collection (two-character abbreviation), year of collection (two-number abbreviation), lake identification number (four-digit code from Appendix A), composite type (one character P = predator species; B = bottom-dwelling species), and sample type (one character S = standard sample; D = duplicate sample). 10. Sign and date the Field Record Form. 11. Remove each fish retained for analysis from the clean holding container(s) (e.g., livewell) using clean nitrile gloves. Dispatch each fish using a clean wooden bat (or equivalent wooden device). 12. Wrap each fish in extra heavy-duty aluminum foil (provided by the sample control center as solvent-rinsed, oven-baked sheets). 13. Prepare a Sample Identification Label (Figure 2) (in black ink) for each sample, ensuring that the label information matches the information recorded on the Field Record Form. 14. Cut a length of food grade tubing (provided by sample control center) that is long enough to ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 6 of 9 contain each individual fish and to allow extra length on each end to secure with cable ties. Place each foil-wrapped specimen into the appropriate length of tubing. Seal each end of the tubing with a plastic cable tie, and attach the appropriate Sample Identification Label. 15. Double-bag each entire specimen package, that is, place inside a large plastic bag with all specimens of the same species from that site and seal with another cable tie. 16. As soon as each sample is packaged, place it immediately on dry ice for shipment. If samples will be carried back to a laboratory or other facility to be frozen before shipment, wet ice can be used to transport wrapped and bagged fish samples in the coolers to a laboratory or other interim facility. 17. If possible, keep all (five) specimens designated for a particular composite in the same shipping container (ice chest) for transport. 18. Samples may be stored on dry ice for a maximum of 24 hours. Sampling teams have the option, depending on site logistics, of: shipping the samples packed on dry ice in sufficient quantities to keep samples frozen for up to 48 hours, via priority overnight delivery service (e.g., Federal Express), so that they arrive at the sample preparation laboratory within less than 24 hours from the time of sample collection, or freezing the samples within 24 hours of collection at <-20°C, and storing the frozen samples until shipment within 1 week of sample collection (frozen samples will subsequently be packed on dry ice and shipped to the sample preparation laboratory via priority overnight delivery service). 19. Complete a Chain-of-Custody Form (Figure 3). All entries must be in black ink and coincide with specimen/sample information on the Sample Identification Labels and Field Record Forms. 20. Retain one copy of the Chain-of-Custody Form and Field Record Form, place and seal all other copies in a waterproof bag, and enclose the sealed forms in the shipping container (ice chest). 21. Pack each shipping container (completely) with dry ice, secure each container with packaging tape, and seal it (e.g., across the latch of the ice chest) with a Chain-of-Custody Label (provided by the sample control center). Include the signature of the sampler and the date/time sealed (in black ink) on each Chain-of-Custody Label. 22. Ship each container to the laboratory via priority overnight express delivery service, as directed by the USEPA Project Manager or Terra Tech Task Leader. Monitor sample holding time, and factor time required for shipment/delivery to ensure that the preservation and holding criteria described in Step 18 have been met. ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 7 of 9 Figure 1. Field record for fish samples Field Record for National Study of Chemical Residues in Lake Fish Tissue Composite Sample ID: (State) (Year) (Lake ID Number) (Composite type (Sample type Sampling Date : Collection Method(s):. P=predator or 3=standard or B=bottom-dweller) D=duplicate) Collector Name (print and sign): Affiliation: Address: Phone: Site Location Latitude: County: Longitude:. Site Name: Site Description:. Circle one: natural lake, modified natural lake, human-made reservoir, other. Estimated maximum lake depth meters pH surface mid (optional) bottom (optional). Sample Description Fish Species: Specimen # Length (mm)* Location Total Number of Individuals: Date/Time Notes 01 02 03 04 05 Additional Comments: *minimum individual size should be no less than 75% maximum individual size Sampling Site Diagram FORM DISTRIBUTION: White - Tetra Tech Task Leader Yellow & Pink - Sample Prep Lab Gold - Sampler EPA Sample Number (to be assigned by Prep Lab)'. ------- Figure 2. Sample identification label(a). SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 8 of 9 1 Project Name Site Identification Specimen # Composite Sample ID # Date Time Preservative Collected by (a) See Appendix D for key to complete Sample Identification Label. ------- SOP Fish Tissue Sample Collection Final Date: 22 May 2000 Page 9 of 9 FigureS. Chain-of-Custody Form. Q CC O O Q O 1 o re re o> (A re o '5) Ł o m o c o 0) 0) Shaded area for Tt use only: Type of Analyses Requested iz _i o o "o. 1 0 (S) Q H d. Cond/Salinity Chlorine Appearance Log Number O T3 GŁ i-s S5 SJ9UIB1UOD 10 JSdlUnN (M/JO 8AI1BAJ8S8.M ent Contact: Project Manager or Cl Address/Phone: Contact Name/Phone: oi E ro -G '2 D. (D J2 -z. CL Sample Location: O D) ro D. Sample Identification/Station E i- 1o Q Date/Time: Received by: (signature) Date/Time: Relinquished by: (signature) Date/Time: Sampled by: (signature) Date/Time: Received by: (signature) Date/Time: Received by: (signature) Date/Time: Received by: (signature) Q. (0 -e o Q. Ol CD O H CQ Dd (f) Q O reproduced electronically ------- ------- Appendix C Standard Operating Procedure: pH Measurements ------- ------- SOP pH Measurements Final Date: 22 May 2000 Page 1 of 3 Standard Operating Procedure pH Measurement Procedures for a National Study of Chemical Residues in Lake Fish Tissue 444444444444444444444444444444444444444444444444444444444444444444444444444444 Scope and Applicability: This Standard Operating Procedure (SOP) must be followed by all Field Sample Collection Teams involved with the USEPA Office of Water's National Study of Chemical Residues in Lake Fish Tissue. Adherence to the SOP will ensure that field pH measurements will be performed the same way every time, i.e., are standardized, for all sampling participants. Procedures for field pH measurement are presented as sequential steps in the SOP to follow, and include equipment, materials, and methods required to perform field measurements only. Responsibility and Personnel Qualifications: This procedure may be used by any Field Sampling Teams that have been authorized by the USEPA Project Manager or the USEPA Regional/State/ Tribal Fish Sampling Coordinators to collect fish for the National Study of Chemical Residues in Lake Fish Tissue. References: American Public Health Association (APHA). 1995. Part 4500 Ff: pH Value. Pages 4-65 - 4-69 in APHA. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Precautions: Follow usual safety precautions for working in the field. Boats should only be operated by qualified, experienced operators trained in their proper use. Each vessel must be equipped with the appropriate Coast Guard-required safety equipment (including personal floatation devices for each field team member). Caution must be used when deploying pH probes overboard, and deployment should only occur when vessels are stable and not under power. Equipment/Materials: Sampling vessel (including boat, motor, trailer, oars, gas, and all required safety equipment)(a) Coast Guard-approved personal floatation devices Maps of target lakes and access routes Field Record Forms(b) Clipboard pH meter with pH electrode/probe(a) Standard buffer solutions (pH 4, 7, and 10) Calibration cup or beaker Distilled water Calibration log book(a) (a) Selection and exact specifications at the discretion of the experienced on-site fisheries biologist. Provided by the sample control center. ------- SOP pH Measurements Final Date: 22 May 2000 Page 2 of 3 Procedures: GENERAL Procedures provided herein focus on pH probe deployment and pH measurement, not specific component assembly or operation since selection of a particular meter for use is at the discretion of the individual field teams. Field teams are urged to read, understand, and follow the manufacturer's instructions for their pH meter of choice for this study. CALIBRATION 1. Prior to deployment (i.e., at a minimum once daily during field use), the pH meter must be calibrated as per manufacturer's specifications. Calibration procedures presented here are generalized and are not water-specific. 2. Rinse the calibration cup (or beaker) and pH sensor with distilled, deionized water. 3. Pour off the deionized water and fill the calibration cup with pH 4.0 buffer solution, making sure that the pH sensor is completely immersed in buffer solution. 4. Turn the meter display on and monitor pH. When the pH reading has stabilized to a consistent value, note the reading in a calibration log book or field notebook. 5. If the displayed value is different from the buffer/standard, adjust the meter (e.g., via a calibration setting) to match the buffer value. Record the final calibrated pH reading (which must match the buffer value) in the calibration log book. 6. Decant the 4.0 buffer solution from the calibration cup, and rinse the pH sensor and calibration cup with distilled water. 7. Fill the calibration cup with 7.0 buffer solution and repeat Steps 4 through 5. 8. Decant the pH 7.0 buffer solution and rinse the pH sensor and calibration cup with distilled water. Fill the calibration cup with pH 10.0 buffer solution and repeat Steps 4 through 5. 9. Decant the buffer solution and rinse the pH sensor with distilled water. 10. Once the meter is successfully calibrated, lake pH can be measured. Water sample temperature must be similar in temperature to that of the calibration standards (or use and adjust the temperature compensation feature of the meter). ------- SOP pH Measurements Final Date: 22 May 2000 Page 3 of 3 DEPLOYMENT AND SAMPLE MEASUREMENT 11. Deploy the pH sensor by gently lowering the probe into the water. Be sure that the sensor is completely immersed. Specific deployment and measurement instructions for the National Fish Tissue Study requires only one measurement location (with an optional vertical profile) per target lake, and are as follows: a. In waters < 2 meters deep, measure pH at approximately 30 cm (approximately 1 ft) below the surface. b. In waters > 2 meters deep, measure pH at approximately 30 cm below the surface, at mid-depth, and at 30 cm above lake bottom substrates; however if multiple measurements or vertical profiles are not feasible (e.g., due to lake depth, meter probe cord length, etc.), record a single measurement at 30 cm below the surface. 12. When the pH reading has stabilized to a consistent value, record the reading on the Field Record Form (provided by the sample control center) to the nearest 0.1 unit. 13. Repeat Step 12 for mid-depth and near bottom measurements, as appropriate and if feasible (refer to Step 11). 14. Follow manufacturer's instructions for pH meter and electrode storage and maintenance. ------- ------- Appendix D Field Data Element Dictionary ------- ------- Field Data Element Definitions and Instructions Associated with Field Sample Collection Activities for the National Study of Chemical Residues in Lake Fish Tissue Element Affiliation: The Affiliation field (on the Field Record Form) contains the agency, group, or company name of those persons conducting the field effort. Collected by: Collection Method: Collector Name: Composite Sample ID: EPA Sample Number Estimated Maximum Lake Depth: Fish Species: The Collected by field on the Sample Identification Label is synonymous with the Collector Name field on the Field Record Form, and contains the name of the Field Team Leader. The Collection Method field on the Field Record Form contains the listing of sampling gear types used to collect samples. The Collector Name field on the Field Record Form is synonymous with the Collected by field on the Sample Identification Label, and contains the name of the Field Team Leader. The Composite Sample ID field on the Field Record Form and the Sample Identification Label is composed of a ten-character code including state of collection (two-character abbreviation), year of collection (two-number abbreviation), lake identification number (four-digit USEPA code), composite type (one character P = predator species, B = bottom-dwelling species), and qualifier for standard or duplicate composite samples (one character S = standard sample, D = duplicate sample). Please leave this space blank on the Field Record Form. The EPA Sample Number is an analytical tracking number that will be assigned by the sample preparation laboratory to each individual aliquot offish tissue prepared for analysis. The Estimated Maximum Lake Depth field on the Field Record Form stores a depth estimate in meters for the deepest portion of the target lake. Estimates will be made by the Field Team and may be based on sources such as lake maps, depth sounder readings, anchor line lengths, etc. The Fish Species field on the Field Record Form stores the common name of the fish retained for analysis. Scientific name entries are optional. ------- Length: Location: Preservative: Project Name: Sampling Date: Site Description: Site Identification: Site Name: Specimen #: Time: The Length field on the Field Record Form contains the individual Total Length (in millimeters) of each fish retained for analysis. Total length of each specimen is measured from the anterior-most part of the fish to the tip of the longest caudal finray (when the lobes of the caudal fin are depressed dorsoventrally) and recorded to the nearest mm. The Location field on the Field Record Form stores a brief description of the area in the lake where each fish was collected. The Preservative field on the Sample Identification Label stores information on how the samples were preserved for shipment, i.e., either on dry ice or frozen. The Project Name field on the Sample Identification Label contains the designation "USEPA National Fish Tissue Study". The Sampling Date field on the Field Record Form and Sample Identification Label stores the numerical month/date/year (e.g., 10/02/99) of sample collection. The Site Description field on the Field Record Form contains a brief written description of the location of the lake (e.g., road or town landmarks) and area of lake sampled (e.g., east portion of the lake). The Site Identification field on the Sample Identification Label is synonymous with the Site Name field on the Field Record Form, and contains the lake name. The Site Name field on the Field Record Form is synonymous with the Site Identification field on the Sample Identification Label and contains the lake name. The Specimen # field on the Field Record Form and the Sample Identification Label consists of a two-digit number from 01 through 05. The Time field on the Sample Identification Label and Field Record Form stores time of sample collection recorded in military time (i.e., four digits). Time fields on the Field Record Form include collection times for each individual specimen. ------- |