OSWER 9240.0-47
                                                          EPA 540-R-09-03

                                                             January 2011
Office of Superfund Remediation and Technology Innovation
                 Contract Laboratory Program
                  Guidance for Field Samplers
Disclaimer: This version of the document replaces any prior versions of the document in their entirety.

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                                             Foreword

This guidance document is designed to provide users with general information regarding environmental sample collection
for the United States Environmental Protection Agency's (USEPA) Contract Laboratory Program (CLP). This document
provides minimum CLP requirements, an explanation of the general sampling process sequence of events, and any related
information.  The appendices contain  useful reference information and checklists to aid  in planning and documenting
sampling activities.

CLP users also are encouraged to review the Introduction to the Contract Laboratory Program document that contains a
general overview of the  CLP, how it works, and how to access the  program. The  CLP requires samplers to use the
functionality provided by either the Field Operations and Records Management System (FORMS) II Lite™ software or
Scribe software, which are the preferred means of creating CLP sample documentation. For guidance in using the software
to record and submit sampling data, users should reference the FORMS II Lite User's Guide  or Scribe reference materials.

The  FORMS II Lite User's Guide, software, and training module can be downloaded from the CLP Web site at the
following address:

                            http://www.epa.qov/superfund/proqrams/clp/f2lite.htm

The Scribe software can be accessed from the USEPA Environmental Response Team (ERT) at the following address:

                                http://www.ertsupport.orq/scribe home.htm

The ERT User Manual for Scribe, reference, and training materials can be accessed from the Scribe Support Web site at the
following address:

                                        http://www.epaosc.orq/scribe

Both the Introduction to the  Contract Laboratory Program and the Contract Laboratory Program Guidance for Field
Samplers can be downloaded from the  CLP Web site at the following address:

                          http://www.epa.qov/superfund/proqrams/clp/quidance.htm

For more information regarding the CLP or this guide, please contact Eric Reynolds via email at revnolds.eric(@,epa.gov or
via telephone at (703) 603-9928.
                                             Key Information

                                Text in     and underlined indicates an external
                                link to information outside of this document.
                                The images below are located throughout the
                                document to draw attention to important
                                information and each are labeled accordingly:
                                                     Important
                                                     Note

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                                            Table of Contents
1.0   INTRODUCTION	1
      1.1   About this Guide	1
      1.2   Overview of the CLP	1
            1.2.1  Key Players Within the CLP	1
      1.3   Overview of the Sampling Process	3
            1.3.1  Procedures Must Be Consistent	3
            1.3.2  Analytical Data Must Be Accurate and Defensible	3
            1.3.3  Sampling Procedures and Guidelines Must Meet Minimum Requirements	4
      1.4   Overview of Sampling Documentation Requirements	4
            1.4.1  FORMS II Lite	4
            1.4.2  Scribe	5
            1.4.3  CLP Documentation Requirements	5
2.0   PRE-FIELD ACTIVITIES	9
      2.1   Prepare for a Sampling Event	9
      2.2   Communicate During a Sampling Event	10
      2.3   Review Project Plans Containing Regional Requirements	10
      2.4   Plan to Meet Documentation Requirements	11
            2.4.1  Request Scheduling of Analysis, SMO-assigned CLP Case Numbers, CLP Sample
                  Numbers, and Laboratory Contact Information	11
            2.4.2  Prepare Sample Cooler Return Documentation	13
      2.5   Obtain Municipal Permits, Licenses, and Clearances	13
            2.5.1  Request Access to County, State, Tribal, Military, and/or Federal Property	13
            2.5.2  Contact Private Property Owners	14
            2.5.3  Contact Utility Companies	14
      2.6   Identify and Obtain Sampling Materials	14
            2.6.1  Procure Appropriate Equipment and Supplies	14
            2.6.2  Procure Sample Containers	15
            2.6.3  Procure Shipping Supplies	16
      2.7   Comply with Transportation and Shipping Requirements	16
      2.8   Provide Shipment Notification	16
      2.9   Perform Readiness Review/Dry Run	17
3.0   IN-FIELD ACTIVITIES	19
      3.1   Collect Samples	19
            3.1.1  Determine Types of Samples to be Collected	19
            3.1.2  Meet Volume, Preservation, and Holding Time  Requirements	22
      3.2   Complete Documentation	27
            3.2.1  Identify a Sample with a CLP Sample Number and SMO-assigned CLP Case Number	27
            3.2.2  Complete TR/COC Records	27
            3.2.3  Complete and Attach Custody Seals	39
            3.2.4  Complete and Attach Sample Labels	39
            3.2.5  Complete and Attach Sample Tags	40
      3.3   Provide Sample Receipt	41
      3.4   Pack and Ship Samples	42
            3.4.1  Sample Containers	42
            3.4.2  Inventory of Samples and Documentation	43
            3.4.3  Shipping Regulations	43
            3.4.4  Sample Packaging for Shipment	43
            3.4.5  Shipment Notification	46
            3.4.6  Return Sample Shipping Coolers	47
Appendix A: Functions within a Sampling Project	A-l
Appendix B: CLP Sample Collection Guidelines for VOAs in Soil by SW-846 Method 5035A	B-l
Appendix C: General CLP  Sample Collection Guidelines VO As in Water	C-l
Appendix D: Sampling Techniques and Considerations	D-l
Appendix E: Sampling Checklists	E-l
             Appendix E-l: Personnel Preparation Checklist	E-l
             Appendix E-2: General Sample Collection Checklist	E-2

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                                         Table of Contents (Cont.)
              Appendix E-3: Completing Field Logbook Checklist	E-3
              Appendix E-4: Completing Handwritten Sample Labels Checklist	E-4
              Appendix E-5: Completing Handwritten Sample Tags & Custody Seals Checklists	E-5
              Appendix E-6: Packing Sample Container Checklist	E-6
              Appendix E-7: Packing Shipping Container Checklist	E-7
              Appendix E-8: Shipping & Reporting CLP Samples Checklist	E-8
Appendix F: Glossary	F-l
Appendix G: FORMS II Lite Analysis Codes	G-l
Appendix H: Scribe CLP Analysis Codes	H-l
                                              List of Figures

Figure 3-1. Packaged Sample with Identification and Chain-of-Custody Documentation (Excluding TR/COC
           Record)	27
Figure 3-2. FORMS II Lite Organic Traffic Report & Chain of Custody Record (Laboratory Copy)	30
Figure 3 -3. FORMS II Lite Inorganic Traffic Report & Chain of Custody Record (Laboratory Copy)	31
Figure 3-4. FORMS II Lite Organic Traffic Report & Chain of Custody Record (Region Copy)	32
Figure 3-5. FORMS II Lite Inorganic Traffic Report & Chain of Custody Record (Region Copy)	33
Figure 3-6. Scribe Organics Chain of Custody Record (Laboratory Copy)	34
Figure 3-7. Scribe Organics Chain of Custody Record (Region Copy)	35
Figure 3-8. Scribe Inorganics Chain of Custody Record (Laboratory Copy)	36
Figure 3-9. Scribe Inorganics Chain of Custody Record (Region Copy)	37
Figure 3-10. Custody Seal	39
Figure 3-11. Completed Sample Tag	40
Figure 3-12. Sample Receipt Created Using the FORMS II Lite Software	41
Figure 3-13. Sample Receipt Created Using the Scribe  Software	42
Figure 3-14. Sample Cooler with Attached TR/COC Record, PES Instructions (if applicable), and Cooler Return
           Documentation	44
Figure 3-15. FORMS II Lite Sample Weight Log	45
Figure 3-16. Scribe Sample Weight Log	45
Figure 3-17. Shipping Cooler with Custody Seals	46
	List of Tables	
Table 1-1. Participants in the CLP Sampling Process	2
Table 2-1. CLP Sample Number Letter Codes	12
Table 2-2. Sample Container Type Specifications	15
Table 3-1. Sample Types and CLP Submission Requirements	20
Table 3-2. Sample Collection Requirements for CLP SOW SOM01.X(VOAs)	23
Table 3-3. Sample Collection Requirements for CLP SOW SOM01.X (SVGAs, Pesticides and Aroclors)	24
Table 3-4. Sample Collection Requirements for CLP SOW ISM01.X	26
Table 3-5. Completing and Attaching a Custody Seal	39
Table 3-6. Completing and Attaching a Handwritten Sample Tag	40
Table 3-7. Packing Samples for Shipment	44
Table D-l. Mixing a Sample and Filling Sample Containers	D-2

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                                            List of Acronyms
ARO
ASB
CERCLA
CLP
CLPPO
CRQL
CVAA
DOT
DQO
dbf
ESDS
ERT
ET
FORMS II Lite™
FSP
HCN
IATA
ICP-AES
ICP-MS
MA
MS
MSB
NaHSO4
NPL
OSC
OSHA
OSRTI
OSWER
PAHs
PCBs
PE
ppb
ppt
PRP
PT
PTFE
PVC
QA
QAPP
QASPER
QATS
QC
RAS
RPM
RSCC
SAM
SAP
SARA
SBG
SMC
Aroclor
Analytical Services Branch
Comprehensive Environmental Response, Compensation, and Liability Act
Contract Laboratory Program
CLP Project Officer
Contract Required Quantitation Limit
Cold Vapor Atomic Absorption
Department of Transportation
Data Quality Objective
Database Format File
Electronic Sample Documentation System
Environmental Response Team (USEPA)
Eastern Time
Field Operations Records Management System II Lite
Field Sampling Plan
Hydrocyanic acid
International Air Transport Association
Inductively Coupled Plasma-Atomic Emission Spectroscopy
Inductively Coupled Plasma-Mass Spectrometry
Modified Analysis
Matrix Spike
Matrix Spike Duplicate
Sodium Bisulfate
National Priorities List
On-scene/on-site Coordinator
Occupational Safety and Health Administration
Office of Superfund Remediation and Technology Innovation
Office of Solid Waste and Emergency Response
Fob/cyclic Aromatic Hydrocarbons
Polychlorinated Biphenyls
Performance Evaluation
Parts-Per-Billion
Parts-Per-Trillion
Potentially Responsible Party
Proficiency Testing
Polytetrafluoroethylene
Polyvinyl Chloride
Quality Assurance
Quality Assurance Project Plan
Quality Assurance Sampling Plan for Environmental Response
Quality Assurance Technical Support
Quality Control
Routine Analytical Services
Remedial Project Manager
Regional Sample Control Center Coordinator
Site Assessment Manager
Sampling Analysis Plan
Superfund Amendments and Reauthorization Act
Sample Delivery Group
System Monitoring Compound

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                                           List of Acronyms
SMO                Sample Management Office
SOP                 Standard Operating Procedure
SOW                Statement of Work
SVGA               Semivolatile Organic Analyte
TR/COC             Traffic Report/Chain of Custody
txt                   Text File
UN                  United Nations
USEPA              United States Environmental Protection Agency
VGA                Volatile Organic Analyte
XML                extensible Markup Language
                                                   IV

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                                                                      Chapter 1 - Introduction to this Guide
1.0    INTRODUCTION


1.1    About this Guide

        This document describes the important organizational roles and responsibilities for those who plan and conduct
        environmental sample collection projects for analysis through the  Superfund's Contract Laboratory Program
        (CLP). This chapter  introduces the structure and purpose of this  document.  Chapter 2, Pre-field Activities,
        addresses pre-field planning activities that the sampling team could complete prior to the actual sampling event.
        Chapter 3, In-field Activities, addresses those activities that need to be completed during the sampling event.

        Appendix A describes the functions within a sampling project which are taken from the Quality Assurance Project
        Plan (QAPP) requirements. Appendix B  and Appendix C contain the  sample collection guidelines for Volatile
        Organic  Analytes (VOAs)  in soil  and in water. Appendix D recommends sampling  techniques. Appendix E
        contains  checklists to help the sampler ensure that all necessary steps are completed.   Appendix F contains the
        glossary  of terms.  Appendix G contains the FORMS II Lite analysis codes. Appendix H contains the  Scribe
        analysis codes.

               A project and site-specific QAPP providing Regional guidance will override guidance given within this
               document.

1.2    Overview of the CLP

        The CLP is  a national network of USEPA personnel, commercial laboratories,  and support contractors whose
        fundamental mission is  to provide data of known and documented  quality.  The CLP  supports USEPA's
        Superfund program which was established under the Comprehensive Environmental Response, Compensation,
        and Liability Act (CERCLA) of 1980 and presently exists under the Superfund Amendments and Reauthorization
        Act (SARA) of 1986. The CLP is directed by the USEPA Analytical  Services  Branch (ASB) from within the
        Office of Superfund Remediation and Technology Innovation  (OSRTI) in the  Office of Solid Waste and
        Emergency Response (OSWER).

        The CLP primary service is the provision of analytical data of known and documented quality to CLP customers
        through its routine and modified chemical analytical services.  The CLP has implemented supporting services to
        ensure that  data of known and documented quality  is provided to  CLP users.  Because of  its  supportive
        infrastructure, the CLP is able to provide all services in a cost-effective and efficient manner. To achieve this
        goal, the CLP has established strict Quality Control (QC) procedures and detailed documentation  requirements.
        Current CLP users include the USEPA Regions,  States and Tribal governments, and other Federal agencies. CLP
        users also are encouraged to review the Introduction to the Contract Laboratory Program document that contains
        a general overview of the CLP, how it works,  and how to access the program.

       1.2.1   Key Players Within the CLP

               In coordinating  Superfund sampling efforts, the  ASB is supported by the Sample Management  Office
               (SMO)  contractor,  Regional CLP  Project Officers  (CLP POs), Regional  Sample Control  Center
               Coordinators (RSCCs), Site Assessment Managers (SAMs), On-scene/On-site Coordinators (OSCs), and
               Remedial Project Managers (RPMs). Samplers may work directly with the RSCC, and/or  an OSC from
               the Site Support Personnel during a sampling event. See Table 1-1 for a brief description of the functions
               performed by key participants (functions may vary by Region).
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Chapter 1 - Introduction to this Guide
                              Table 1-1. Participants in the CLP Sampling Process
         Participants
                                  Responsibilities
Analytical Services Branch
USEPA ASB directs the CLP from within the Office of Superfund Remediation and Technology
Innovation (OSRTI) in the Office of Solid Waste  and Emergency Response (OSWER). ASB
responsibilities include:
•  Development of the Statements of Work (SOWs) that define required analytical methods
   (including QC, detection/quantitation limits, and holding times) for the analytical services
   procured under the CLP;
•  Development and  implementation of  policies  and budgets  for  Superfund analytical
   operations;
•  Development of information management policies and products for analytical data;
•  Management of SMO and Quality Assurance Technical Support (QATS) contracts;
•  National administration, evaluation, and management of the CLP; and
•  Direction of CLP Quality Assurance (QA) activities  in coordination with overall OSWER
   QA activities.
To obtain the most current ASB contact list, refer to  the following Web site:
            http://www.epa.qov/superfund/proqrams/clp/contacts.htmtfASB
CLP Sample Management Office
The contractor-operated SMO provides necessary management, operations, and administrative
support to the CLP.  SMO receives Regional analytical requests, coordinates and schedules
sample  analyses,  and tracks sample  shipments.  SMO also receives  and checks data for
completeness and compliance, processes laboratory  invoices, and maintains a repository of
sampling records and program data.
CLP Contract Laboratories
The contractor-operated laboratories within CLP provide necessary analytical services for the
isolation, detection, and quantitation of the CLP's target compounds and analytes. To obtain the
most current list of CLP Contract Laboratories, refer to the following Web site:
           http://www.epa.qov/superfund/proqrams/clp/download/lablist.pdf
Regional CLP Project Officer
The CLP PO monitors the technical performance of the contract laboratories in each Region.
The CLP PO works closely with ASB Program Managers to identify and resolve laboratory
technical issues, and leads laboratory on-site evaluations. To obtain the most current CLP PO
contact list, refer to the following Web site:
                http://www.epa.qov/superfund/proqrams/clp/reqlist.htm
Regional Sample Control Center
Coordinator
In most Regions, the RSCC coordinates sampling efforts and serves as the central point-of-
contact for sampling questions and problems. The RSCC works with SMO to schedule sample
shipments to laboratories. In addition, the RSCC's activities may include: informing  SMO of
sample shipment, cancellations, special instructions, and sampling issues. To  obtain the most
current RSCC contact list, refer to the following Web site:
                http://www.epa.qov/superfund/proqrams/clp/reqlist.htm
Site Support Personnel
The Site Support Personnel consists of the USEPA personnel responsible for developing the
Quality Assurance Project Plan (QAPP) and Sampling Plan for the sampling episode at the site,
such as  the sampling team, Quality Assurance personnel, OSC, SAM, and Remedial  Project
Manager (RPM). In most Regions, the Site  Support Personnel develops Standard Operating
Procedures (SOPs) for field sampling and related procedures, and assists sampling teams in
following those SOPs. The sampling team determines what type(s) of CLP  services  will  be
required for a particular sampling event. The Site Support Personnel reviews Sampling Analysis
Plans (SAPs) prepared by sampling teams and oversees sampling teams in the field.
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                                                                        Chapter 1 - Introduction to this Guide
1.3    Overview of the Sampling Process

        Once  USEPA has  determined  that  physical,  chemical,  and/or
        biological testing of a site is necessary, samples of material from the
        site area must be  collected.  The type of material  that must  be
        collected and the analytical method to be used  depends upon the
        physical location  of the site, detection level(s), site history (previous
        sampling), and known or unknown conditions and contaminants. The
        sampling process  includes carefully planned and consistently applied
        procedures that produce accurate and legally defensible data. The
        sampling team should consider the procedures and plans presented in
        this guide as minimum sampling process guidelines to maintain
At-a-Glance:
Overview of the Sampling Process

•/  Procedures must be consistent.
•S  Analytical data must be accurate and
    defensible.
•S  Procedures  must  meet  minimum
    requirements.
        sample integrity and identity.  Samples should be collected according to the approved project and site-specific
        QAPP and SAP. This document does not define specific sampling procedures because specific sampling protocols
        depend on individual site conditions, Regional requirements, and acceptance  and performance criteria.  Since
        Regions may have their own specific requirements for individual sampling programs, they are responsible for
        generating Region-specific sampling SOPs.

       1.3.1   Procedures Must Be Consistent

                The purpose of sampling is to collect representative portions from a suspected contaminated site. Sample
                collection is critical to determining the presence, type, concentration, and extent of environmental
                contamination by hazardous substances; thus it is a  crucial part of every sampling and environmental
                testing effort. Sampling procedures must be consistently written and followed to mitigate risk of error and
                the expense of re-sampling.

                Failure to follow proper sampling and shipping procedures could result in samples that are contaminated,
                broken, mislabeled, lost during shipping, or unusable because of a missed holding time. If procedures are
                inconsistently or improperly followed, any resultant analytical data may be inaccurate and may not be
                defensible in a court of law.
                         If re-sampling is needed due to improper sampling, the sampling team may incur the cost.
       1.3.2   Analytical Data Must Be Accurate and Defensible

                The data gathered during sampling activities helps to accurately characterize contaminated waste sites so
                that the impact on human health and the environment can be properly evaluated. Acquiring accurate and
                defensible data that will be accepted in a court of law is the CLP's primary objective; therefore, the
                sampler must collect samples according to strict sampling procedures, plans, and guidelines. USEPA and
                many other Federal agencies use data resulting from analytical testing of samples to:

                •   Determine if a site is contaminated with organic and/or inorganic compounds
                •   Identify pollution sources and Potentially Responsible Parties (PRPs)
                •   Validate remedial design methodologies
                •   Assess response and remedial priorities
                •   Assess risk to human health and the environment
                •   Determine appropriate cleanup actions
                •   Determine cleanup achievements
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Chapter 1 - Introduction to this Guide
       1.3.3   Sampling  Procedures and Guidelines Must Meet Minimum Requirements

               It is imperative that samplers be aware of the minimum CLP and Regional requirements that directly
               impact and define how a sampling event will take place. It is important to note that the procedures and
               guidelines set forth in this document are considered minimum CLP requirements.  Samplers should
               reference the following sections within this document that specifically address important requirements
               that must be met for a successful sampling event:

               •   Section 1.4.3 CLP Documentation Requirements
               •   Section 2.4.1 Request Scheduling of Analysis, SMO-assigned CLP Case Numbers, CLP Sample
                   Numbers, and Laboratory Contact Information
               •   Section 2.7 Comply with Transportation and Shipping Requirements
               •   Section 2.8 Provide Shipment Notification
               •   Section 3.1 Collect Samples
               •   Section 3.2 Complete Documentation

1.4    Overview of Sampling Documentation Requirements
       The sampler must properly  document samples collected for
       analysis in order to uniquely identify each sample and ensure
       adequate chain-of-custody procedures. When collecting samples,
       the sampler should always keep  in  mind that any samples
       collected may be used in  future litigation. This is  especially
       important when samples are from privately owned property. If
       sampling on privately owned property, samplers  should also
       provide the property owner with a receipt for samples collected
       and removed from that owner's property. Samplers may also be
       required by a Region to use a sample label, sample tag, or field
       operations  records  documenting information  such as  daily
       activities, equipment and materials used, personnel involved, site
       security, etc. These types of documentation help ensure proper
       sample  identification and provide additional chain-of-custody
       records.

       The documentation required by a Region for a sampling event is
       outlined in project plans such as the QAPP, SAP,  and Field
       Sampling Plan (FSP).
At a Glance:
Overview of the Sampling Document
Requirements

•S   Must use FORMS II Lite or Scribe to create
    sample documentation. Analytical data must
    be accurate and defensible.
•S   CLP documentation requirements:
    -  CLP Sample number
    -  SMO-assigned CLP Case number
    -  Traffic Report/Chain of Custody
      (TR/COC) record
    -  Sample labels
    -  Sample tags
    -  Custody seals
    -  Field operation records
                 Under no circumstances should the site name appear on any documentation that is sent to the laboratory
                 (for the CLP).
       The two sampling documentation software tools prescribed and used by the CLP are: Field Operations Records
       Management System II Lite (FORMS II Lite) and Scribe.

       1.4.1   FORMS II Lite

               In an effort to automate sample documentation in the field, ASB has developed a stand-alone, Windows-
               based software application that  samplers  can use  to  automatically create and  generate sample
               documentation. The  FORMS II Lite software allows  users to enter information prior to and during
               sampling events.  It allows users to multi-task and  electronically create, edit, and print documentation
               associated with sampling activities.  Users  can customize data entry  screens throughout the  entire
               documentation process.  Users can also customize  the format and content of  sample labels based on
               specific requirements.
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                                                                       Chapter 1 - Introduction to this Guide
                The program simplifies and accelerates the tedious manual sample documentation process by reducing
                the generation of handwritten documents by almost 70%. The FORMS II Lite software enables samplers
                to:

                •    Augment CLP-provided Sample numbers or manually assign their own unique, project-specific non-
                    CLP Sample numbers
                •    Input the SMO-assigned CLP Case Number into the appropriate field
                •    Create sample labels, sample tags, TR/COC Records, Sample Weight forms, and receipts for samples
                    taken from a site
                •    Track samples from the field to the laboratory
                •    Electronically capture sample information into databases
                •    Export electronic data as a  database  format (.dbf) file, text (.txt), or extensible Markup Language
                    (.xml) file

                For assistance with obtaining or using the FORMS II Lite software, please contact the FORMS II Lite
                Help Desk at 703-818-4200 from 9:00 AM - 5:00 PM Eastern Time (ET). For additional information
                regarding FORMS II Lite use and training, please refer to the following Web site:

                                    http://www.epa.qov/superfund/proqrams/clp/f2lite.htm

       1.4.2   Scribe

                In an effort to automate sample  documentation in the field, the USEPA Environmental Response Team
                (ERT) has developed  a stand-alone,  Windows-based  desktop application that samplers can use to
                automatically create  and generate sample documentation.  The Scribe  software allows users  to enter
                information prior to and during  sampling events. It allows users to multi-task and electronically create,
                edit, and print documentation associated with sampling activities. Users can customize data entry screens
                throughout the entire documentation process. Users can also customize the format and content of sample
                labels based on specific requirements.

                The Scribe application simplifies and accelerates the tedious manual sample  documentation process by
                reducing the generation of handwritten documents.  Scribe enables samplers to:

                •    Augment CLP-provided Sample numbers or manually assign their own unique, project-specific non-
                    CLP Sample numbers
                •    Input the SMO-assigned CLP Case Number into the appropriate field
                •    Electronically capture sample information into databases
                •    Create sample labels, sample tags, COC Records, and receipts for samples taken from a site
                •    Track samples from the field to the laboratory
                •    Electronically capture laboratory results, property details and sample location details
                •    Export electronic data as a database format (.mdb) file, text (.txt), or extensible Markup Language
                    (.xml) file

                The Scribe CLP Analysis Codes provided in Appendix H may cause a delay in processing at the CLP
                laboratories based on interpretation.

                          Samplers are advised to use the analysis codes found in Appendix G: FORMS II Lite
                          Analysis Codes for CLP analysis.

                Scribe users should contact the  ERT Software Support Help Desk for assistance in adding the custom
                analysis onto Scribe. For assistance with obtaining or using Scribe  software, please contact the ERT
                Software Support Help Desk at 800-999-6990. For  additional information  regarding Scribe  use  and
                training materials, please refer to the following Web site:

                                        http://www.epaosc.orq/Scribe

       1.4.3   CLP Documentation Requirements

                Samplers must:

                1)  Record the CLP Sample Number and the SMO-assigned CLP Case Number on each sample bottle

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Chapter 1 - Introduction to this Guide
               2)  Complete the Traffic Report/Chain of Custody (TR/COC) record using the FORMS II Lite or Scribe
                   software, making sure to indicate on the TR/COC record if the samples require the use of a Modified
                   Analysis
               3)  Complete and attach sample labels
               4)  Complete and attach sample tags to meet Regional requirements (if applicable)
               5)  Complete and attach custody seals to meet Regional requirements
               6)  Complete field operations records,  as necessary

               Please contact your RSCC (see Table 1-1)  for information regarding CLP Sample numbers, SMO-
               assigned CLP Case numbers, TR/COC records, and chain of custody seals for sampling events.

               1.4.3.1   CLP  Sample Number

                         A CLP Sample Number is unique per sampling location, is used to identify and track samples
                         throughout the sampling and analytical processes, and is recorded on many types of sampling
                         documentation (e.g.,  TR/COC  Records, sample  labels,  and  sample tags).  CLP Sample
                         Numbers are provided to samplers by their RSCC or SMO. The CLP  Sample Number should
                         not contain the letters "I," "O," "U," and "V."

                         Samplers must contact their RSCC (or designee)  to obtain CLP Sample numbers for their
                         sampling event. Samplers must correctly assign the CLP Sample numbers to the appropriate
                         sample bottle or container. Please refer to  Section 3.2.1 for more  detailed  information
                         regarding the use of CLP Sample Numbers.


                                Contact the RSCC with any questions regarding the assignment of CLP sample and
                                SMO-assigned Case numbers.


               1.4.3.2  SMO-assigned CLP  Case Number

                         SMO-assigned CLP Case  numbers  are  used to  track groups  of samples throughout the
                         sampling and analytical processes and are recorded on many types of sampling documentation
                         (e.g., TR/COC records, sample labels,  and/or sample tags). Samplers must correctly assign the
                         SMO-assigned CLP Case number to the appropriate sample bottle or container.  To obtain a
                         SMO-assigned CLP Case number, samplers may contact their RSCC (or designee) to obtain
                         their laboratory assignment notification or they may be provided by SMO.

               1.4.3.3  Laboratory Assignment

                         Samplers are responsible for shipping samples to the appropriate SMO-assigned laboratory for
                         analysis. Samplers may contact their RSCC (or designee) to obtain their laboratory assignment
                         notification or they may be provided by SMO.

               1.4.3.4  TR/COC Record

                         The TR/COC record  is used as physical evidence  of sample  custody and functions as a
                         permanent record of each sample collected.

                         Per CLP documentation requirements,  each cooler must contain a TR/COC record that lists all
                         the samples contained therein.

               1.4.3.5  Chain-of-Custody Seals

                         A chain-of-custody seal is any adhesive label or tape that can be used to seal a sample bottle,
                         container, plastic bag, or shipping cooler such that if it is opened or tampered with, the seal
                         will be broken.  Custody seals must be placed on each sample bottle, container, or bag (as
                         appropriate) and each shipping cooler  or container. The custody seal is an excellent means of
                         maintaining a  record  of chain-of-custody, as well as  guarding against possible sample
                         contamination or tampering  during shipping.

               1.4.3.6  Sample Labels

                         A sample label is a sticker attached to a sample bottle or container that contains a field sample
                         or QC sample. Sample labels are affixed to each sample container as samples are collected in

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                                                                        Chapter 1 - Introduction to this Guide
                         the field or affixed prior to going in the field. A sample label must contain, at a minimum, a
                         CLP  Sample number so that they can be  associated with, and listed  on, the associated
                         TR/COC record. The sample label should also include the required analysis, Case number, and
                         preservative used (to eliminate confusion at the  laboratory). Samplers should  refer to their
                         project plans for Region-specific sample label requirements.

                1.4.3.7 Sample Tags

                         A sample tag identifies a sample bottle or  container that contains a sample.  The tag also
                         provides specific  analytical direction and proof that a sample existed. To support the  use of
                         sample data in potential enforcement actions, samples with other than in situ measurements
                         (e.g., pH, temperature, conductivity) can  be identified with a  sample tag. A  CLP Sample
                         number and SMO-assigned CLP Case number must be recorded on a sample tag to indicate
                         that the sample container comprises the whole sample in the case where there is just one
                         container  of sample, or part of the indicated  sample  in the case of multiple  containers of
                         sample.  Samplers should refer to their project  plans  for Region-specific  sample tag
                         requirements.

                1.4.3.8 Field Operation Records

                         Samplers  should  maintain complete, accurate, and  legible field operations records as they
                         perform a sampling activity.  The following records are included: field logbooks; Corrective
                         Action reports; Sampling trip reports; supplemental standardized forms; logs; and records such
                         as maps or photographs that document each step of the work performed in the field. Samplers
                         should refer to their project  plans for Region-specific field operations record requirements.
                         These records are very important tools because they are considered part of the official project
                         file when legal issues arise.

                1.4.3.9 Weight Logs

                         A sample weight log identifies the tared,  sample  and final weights per bottle  for  VOA
                         samples. In order to support Method 503 5A for VOAs, samplers should enter tared and final
                         weights per bottle in the CLP Sample Weight Log.
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                                                                               Chapter 2 - Pre-field Activities
2.0   PRE-FIELD ACTIVITIES

        This chapter provides instructions for completing the suggested pre-
        field activities that samplers  could complete prior to performing
        sampling activities. These important pre-field  activities  will  save
        time and help the sampler to better prepare for the sampling event.
        Samplers should be aware of issues that routinely arise during the
        sampling process so that they can avoid making the same mistakes or
        having the same problems that could adversely affect their sampling
        event.  Samplers  are also expected to review all pertinent project
        plans and meet both CLP and Regional requirements that  directly
        impact the structure and purpose of a sampling event.

        The project plans provide information such as the types and numbers
        of samples to be collected, the analytical methods to be used based
        on the desired level of quantitation, and the necessary equipment and
        supplies. The plans also describe the sampling method which may
        require  different  specific  sample  volumes/masses,  containers,
        preservation, shipping, and handling to maintain the integrity of the
        samples without degradation or contamination.
At a Glance:
Pre-field Activities

v'   Prepare for and communicate during a
    sampling event.
v'   Review project plans containing
    Regional requirements.
v'   Plan to meet documentation
    requirements.
v'   Obtain any necessary permits, licenses,
    and clearances.
v'   Identify and obtain sampling materials.
•^   Comply with transportation and
    shipping requirements.
•S   Provide shipment notification.
•S   Perform Readiness Review/Run-
    through.
        In addition to reviewing project plans, samplers should determine if the sampling site is privately or publicly
        owned and obtain the necessary permission to access the sampling site. If the site is privately owned, samplers
        should make sure to have receipts available to provide to the owner for all samples collected and removed from the
        property. Samplers must also prepare to identify and obtain sampling materials, prepare to meet documentation
        requirements by obtaining and learning to use the required software, comply with transportation and shipping
        requirements, and perform a readiness review/dry run of the sampling process.

2.1    Prepare for a Sampling Event

        Samplers must prepare to meet CLP  and Regional requirements for a sampling event, appropriately use the CLP
        Sample number and SMO-assigned CLP Case number, complete the TR/COC record using the FORMS II Lite or
        Scribe software, and complete  and attach the custody seal(s). It is very important that the sampler include  the
        correct CLP Sample number on each sample. It is  also imperative that the TR/COC record be accurately
        completed and submitted with the sample(s). Finally, the sampler must accurately and legibly complete and attach
        a custody seal to  each sample container, or  plastic sample bag  (as appropriate), and each shipping cooler or
        container.

        However, meeting the sampling requirements requires more  than just the proper application of a CLP  Sample
        number on each sample, completion  of the TR/COC record, and use of a custody seal.  The actual collection of
        samples, packaging, and shipping of those samples are equally important to a successful sampling event.

        For example, if a sampler collects insufficient volume of a sample, the laboratory may not be able to perform the
        requested analysis. Insufficient sample volumes may also result in a laboratory being unable to perform laboratory
        quality control,  such as  Matrix Spike (MS),  Matrix Spike Duplicate (MSD), and Duplicates  sample analysis.
        Additionally, if the laboratory receives a sample that is either unpreserved or the sample pH is outside of the
        required range, the sample cannot be properly analyzed.

        Unfortunately, improper shipping and labeling processes and procedures often result in:

        •   Samples being shipped to the wrong laboratory
        •   Broken or empty samples being received at the laboratory
        •   Custody seals or sealant tape that is missing or broken on sample bottles, containers, plastic bags, or shipping
            coolers shipped to the laboratories
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Chapter 2 - Pre-field Activities
        The importance of completing the paperwork associated with a sampling event cannot be overemphasized.
        Samplers  must make a conscientious effort to accurately complete the TR/COC  record since this is the main
        document used to derive vital information about a particular sample. The person completing a TR/COC record
        must be careful to avoid errors, such as the appropriate sample(s) not being listed, or the wrong samples being
        listed.  In an effort to eliminate such errors and the confusion that can be associated with handwritten TR/COC
        records, samplers must use either the FORMS II Lite or Scribe software to complete the TR/COC record and other
        associated sampling documentation.

        It is extremely important that QC samples, including field sample duplicates, field  samples for Matrix Spike and
        Matrix Spike Duplicate analyses,  and Proficiency Testing (PT) samples, also known as Performance Evaluation
        (PE) samples, be designated and labeled per Regional guidance by samplers in  the field.  Mislabeling of QC
        samples can result in improper and/or inaccurate analysis of a sample at the laboratory.

2.2    Communicate  During a Sampling  Event

        Communication is a key element in planning, administrating,  and conducting a sampling event. It is extremely
        important that all  parties involved  in  a sampling event be in contact throughout the sampling process. The
        procedures and recommendations outlined in this guide are based on more than 20 years of experience. It has been
        demonstrated that approximately 50% of all sampling efforts have been negatively affected by incorrect sampling
        procedures and poor communication among participants.

        The key elements of communication for a sampling event include the relationship between the RSCC, SMO, the
        samplers in the field, and the laboratories who will be accepting the samples. For instance, the samplers must
        contact the RSCC to start the process for setting up a sampling event. The RSCC will in turn contact SMO who
        will schedule  the  sampling event, establish laboratory availability, and arrange  for the laboratory to accept
        projected  samples. For MAs requesting tissue analysis, it  is also important to notify the laboratory if they should
        be expecting whole fish, fish fillet or other types of tissues for analysis. After scheduling, SMO will communicate
        the laboratory assignment to the Region and possibly the sampler.

        SMO provides SMO-assigned CLP Case and CLP Sample Numbers in time for the sampling event. SMO also
        schedules a laboratory and makes sure the laboratory will not have any capacity problems.  Communication is also
        important because if there is a change in the sampling event due to a cancellation or an increase or decrease in the
        number of samples that will be sent to the laboratory, the sampler can contact the RSCC who can work with SMO
        to remedy potential capacity, availability, or overbooking problems.

2.3    Review Project Plans Containing Regional  Requirements

        In addition to meeting CLP requirements, the sample collection process must fulfill  numerous Regional
        requirements. These requirements are  determined by a  variety of factors that affect how  samples should be
        collected for an individual sampling event. These factors include:

        •    The type of samples being collected (organic/inorganic, water, soil/sediment, etc.)
        •    The method by which the samples will be analyzed
        •    The acceptance or performance criteria [i.e., Data Quality Objectives (DQOs)]
        •    The type of data needed
        The QAPP for each sampling project is written to meet requirements outlined in the documents EPA Requirements
       for Quality Assurance Project Plans (QA/R-5), EPA Guidance on Quality Assurance Project Plans (G-5), and
        Regional QAPP preparation documents. The QAPP is prepared in  advance  of field activities and is used by
        samplers to develop any subsequent plans such as the SAP or the FSP. Samplers should review  the QAPP and any
        subsequent project plans for information outlining the basic components of a sampling activity.  QAPP and project
        plans should be finalized and approved by appropriate Regional QA personnel, the OSC, SAM,  or the RPM before
        providing them to the sampling team. This should be done prior to the  start of field activities. Appendix A explains
        the functions within a sampling project (as these functions relate to  a sampling event) and the elements of that
        function as described in a typical QAPP. Copies of all project plans and relevant SOPs should be maintained in the
        field for the duration of the sampling project.
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                                                                           Chapter 2 - Pre-field Activities
2.4    Plan to Meet Documentation Requirements
        Sampling events require a variety of accurate and complete   ^^^^^^^^^^^^_^^^^^^^^^^^^_
        documentation. Samplers should review their project plans   At a Qjance.
        to determine the  types  of  documentation that must be   Pian to meet documentation requirements.
        completed for a  sampling project and to ensure that the
        appropriate documentation will be on-hand in the field. The   ^  Re1uest SMO-assigned CLP Case and CLP
        CLP documentation requirements include the CLP Sample       Sample Numbers.
        Number,  the  SMO-assigned  CLP  Case Number,  the   ^  Prepare sample cooler return documentation.
        TR/COC Record,  sample labels, custody seals, and  field   ^  Prepare to use the FORMS II Lite or  Scribe
        operations records (as necessary). Samplers need to request       software.	
        SMO-assigned CLP  Case and CLP Sample Numbers for
        each sampling event prior to starting field activities. Samplers also need to make sure that the correct TR/COC
        Records (Organic TR/COC Record for organic analysis or Inorganic TR/COC Record for inorganic analysis) are
        being used within the FORMS II Lite or Scribe software. Finally, samplers should be prepared to complete the
        appropriate shipping cooler return documentation.

        Samplers are required to use the FORMS II Lite or Scribe software to prepare and submit sampling project
        documentation and maintain sample chain-of-custody. Samplers must have access to FORMS II Lite or Scribe
        generated TR/COC Records at sampling events.  FORMS II Lite or Scribe software users must be familiar with all
        emergency backup procedures that should be followed in the event of a system failure. In the event of a system
        crash, samplers must  have backup hardcopies of FORMS II Lite  or Scribe TR/COC Records. For information
        regarding emergency backup procedures, please refer to the following Web site:

                               http://www.epa.qov/superfund/proqrams/clp/trcoc.htm

        For assistance while using the FORMS II Lite software, please contact the FORMS II Lite Help Desk at 703-818-
        4200 from 9:00 AM - 5:00 PM ET.

        For assistance while using the Scribe software, please contact the ERT Software Support Help Desk at 800-999-
        6990 from 9:00 AM - 5:00 PM ET.  Refer to the following web site for additional  information on the use and
        training of Scribe:

                                          http://www.epaosc.orq/Scribe

       2.4.1   Request Scheduling of Analysis, SMO-assigned CLP Case Numbers, CLP
               Sample Numbers, and Laboratory Contact Information

               SMO-assigned CLP Case Numbers are  assigned based on a request for CLP Routine Analytical  Services
               (RAS), which is processed though the RSCC (or designee).  The sampler  must request the RSCC to
               schedule CLP RAS  analysis. The sampler should specify the  number of samples, analyses, etc., being
               shipped each week when requesting the RSCC to schedule CLP RAS analyses.  The CLP does  have the
               capacity to schedule sampling on an emergency basis, however the sampler must contact the RSCC (or
               designee) to obtain details regarding how to handle  such a situation. When scheduling a sampling event
               that will last for more than one week, it is recommended that the sampler contact the RSCC (or designee)
               on a weekly basis to provide updates.  This contact between the sampler, the RSCC (or designee), and
               SMO is very important because it will ensure better availability of laboratory capacity.

               In addition to SMO-assigned CLP Case and  CLP Sample numbers, samplers should make sure to have
               accurate laboratory contact information, such as:

               •   Laboratory name
               •   Laboratory address
               •   Contact name
               •   Laboratory phone number

               This information which is provided on the Regional Lab Assignment Notification Form is used for both
               TR/COC records and chain-of-custody documentation and shipping paperwork such as address labels and
               airbills.
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Chapter 2 - Pre-field Activities
                The SMO-assigned CLP Case number is used to track groups of samples throughout the sampling and
                analytical processes.  Samplers must correctly indicate the  assigned Case number on the appropriate
                sample bottle or container.

                         The RSCC  (or designee) provides the CLP Case numbers and Sample numbers for each
                         sampling event to samplers.  Once the CLP Sample  numbers have been  provided to the
                         sampler, the sampler can use FORMS II Lite or Scribe to print them onto sample labels.

                A CLP Sample number is defined as a number that is unique per sampling location and identifies each
                CLP sample (see Section 1.4.3.1). Since samples must be identified per analytical program (either organic
                or  inorganic), there are two types of TR/COC Records and two  letter codes to denote organic vs.
                inorganic analysis.

                A CLP sample is defined as one discrete portion  of material to be analyzed that is contained at one
                concentration level, from one station location for each individual  or set of analyses ~  provided the
                analyses are all requested for the same CLP analytical service (i.e., organic or inorganic), and identified
                by a unique Sample number.

                         When samples are collected from several station  locations to form a composite sample, the
                         sample should be assigned either a number from one of the station locations used during
                         collection, or a unique number that represents the composite sample, for tracking purposes. The
                         numbering scheme used internally at a  sampling event  for identifying composite samples
                         should also be documented appropriately (e.g., in the field logs).

                Organic  CLP Sample numbers  begin  with the Regional letter  code, followed by four letters and/or
                numbers. Inorganic CLP Sample numbers begin with "M" followed by the Regional letter code and then
                four letters and/or numbers. See Table 2-1 for Region and letter codes for each sample type (i.e., organic
                or inorganic).

                                 Table 2-1. CLP Sample Number Letter Codes
Region
1
2
3
4
5
6
7
8
9
10
Letter Code
Organic
A
B
C
D
E
F
G
H
Y
J
Inorganic
MA
MB
MC
MD
ME
MF
MG
MH
MY
MJ
                According to CLP guidelines, each individual inorganic water sample may be analyzed for total metals or
                filtered metals, but not both. Therefore, water samples collected for total metal and filtered metal analyses
                from the same sampling location must be assigned separate (unique) CLP Sample numbers. A sampler
                can use the same CLP Sample Number for an inorganic soil or water sample collected for total metals,
                mercury and cyanide analyses.

                Organic soil and water samples may be collected for analysis under the SOM01.X SOW to detect:
                •   Aroclors
                •   Semivolatile Organic Analytes (SVGAs)
                •   SVGA Selective Ion Monitoring (SIM)
                •   Pesticides
                •   Volatile Organic Analytes (VOAs)
                •   Trace Volatile Organic Analytes (Trace VOAs)
                •   Trace VGA SIM
12
January 2011

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                                                                              Chapter 2 - Pre-field Activities
               Inorganic soil and water samples may be collected for analysis under the ISM01.X SOW to detect:
               •   Cyanide using Distillation/Colorimetry
               •   Metals using Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES), Inductively
                   Coupled Plasma-Mass Spectrometry (ICP-MS)
               •   Mercury using Cold Vapor Atomic Absorption (CVAA)

               Inorganic wipes  and air filter samples may be collected for metals  using ICP-AES under ISM01.X
               SOW.

       2.4.2  Prepare Sample Cooler Return  Documentation

               CLP laboratories must routinely return sample shipping coolers to the appropriate sampling office within
               14 calendar days following receipt of shipment from the  sampler. For sample coolers to be returned, the
               sampler must complete the appropriate cooler documentation and work with Regions and government
               agencies to provide  a cost-effective mechanism for laboratories to return the empty coolers to the
               appropriate sampling office. The sampling cooler return documentation should be prepared in advance
               and provided to samplers before field activities begin.  The sampler (not  the CLP  laboratory) is
               responsible for paying for the  return of the cooler and should also include  shipping airbills bearing
               the sampler's account number, as well as a return address, to allow for cooler return.

               To maintain consistency among  cooler transportation programs, samplers should:

               •   Minimize the use of multiple transportation carriers to avoid confusion
               •   Use multiple-copy labels so the laboratory and the sampling team can each retain a copy for their
                   records
               •   Prepare labels in advance so that the laboratory can simply affix a completed shipping label on the
                   cooler
               •   Include third-party billing  information (i.e.,  their shipping account number) on labels so the
                   laboratory will not be billed by the transportation carrier
               •   Confirm that the laboratory  knows which transportation carrier to use
               •   Include the SMO-assigned CLP Case Number on return information

2.5    Obtain  Municipal   Permits,   Licenses,   	
        and Clearances
                                                                   Obtain permits, licenses, and clearances.

        Before  starting a sampling event,  samplers must make sure to   ^  Request access to County, State, Tribal,
        obtain the proper municipal  permits, accesses to the property,       military, and/or Federal property.
        and any government clearances, if required. The sampler must   ^  Contact private property owner(s).
        also contact any appropriate utility  companies to ascertain where   •/  Contact utility companies.
        any underground pipes, cables, etc., may be located.               ^^^^^^^^^^^^^^^^^^^^_
       2.5.1    Request Access to County, State, Tribal, Military, and/or Federal Property

                Proper access to perform sampling activities is important not only for legal reasons, but also to eliminate
                delays in work and possible refusal to allow sampling to take place. It  is  crucial that  the appropriate
                permits, licenses, and clearances be secured to obtain access for sampling activities that will be performed
                on County, State, Tribal, military, and/or Federal property. The sampler must contact  the appropriate
                government offices or personnel well in advance to determine what kinds of approval are required. Pre-
                approval may  be  required for specific types of sample collection such  as drilling or excavation.  For
                example, drilling on a military base requires pre-approval. Base security  may require clearances for all
                members of the sampling team, including subcontractors. This process may take two or more days.

                If arrangements are not made in advance,  the team may not  be allowed  to enter the site until their
                clearances are  processed and the team has been approved to drill. As a result, the sampling schedule is
                delayed, costing extra time and money.
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Chapter 2 - Pre-field Activities
       2.5.2  Contact Private Property Owners

               The sampler must obtain written permission from the private property owner(s) before sampling on their
               property, even if verbal permission has been granted.  It is recommended that  samplers obtain verbal
               permission prior to their arrival at the sampling location, but written permission can be obtained on the
               day of sampling. If a property owner refuses to  grant access to their property, it may be necessary for
               sampling participants to contact the appropriate authorities for assistance.

       2.5.3  Contact Utility Companies

               The sampler should contact local utility companies (e.g., power, phone, gas, cable, sanitation, etc.) at
               least one week prior to the sampling event to have underground cables, lines, and pipes flagged and
               marked. This is required by law. A national one-call directory can be found at:
                                                  http://www.call811 .com.

               This will eliminate potential safety hazards  and service disruption. For example, soil sampling in a
               residential area may require digging below the soil's surface. It is very important to know where utility
               lines and pipes are located so that samplers do not hit live electrical wires or rupture gas lines. Samplers
               should follow Regional or  other appropriate program procedures  for the procurement of such services.
               The utility service(s) disruption dates should be confirmed at least two days prior to sampling activities.
                        Pre-payment of survey fees to local utility companies may be required.


2.6    Identify and Obtain Sampling Materials
                                                                     Identify and obtain sampling materials.
        Samplers must  be prepared for  a sampling project  with the   ,  Procure appropnate equipment and
        appropriate  sampling  materials  (equipment,  supplies,  sample       supplies
        containers,  packing materials, and shipping materials).  The   j  procure sample containers
        equipment and supplies must be properly cleaned, calibrated, and   ^  Procure shipping supplies.
        tested as necessary to meet the needs of the sampling project.         	
       2.6.1    Procure Appropriate Equipment and Supplies

                Each sampling event requires the procurement of equipment and materials to collect, document, identify,
                pack, and ship samples. The proper field sampling equipment is vital to a successful sample collection.
                Regional or other samplers should obtain, and arrange in advance, all of the equipment and supplies
                required for each sampling event.  Samplers  should review the project plans to verify that the proper
                equipment is being used for sample collection.

                At a minimum, the following materials are generally required during a sampling event:

                •    Sample storage containers
                •    Packing material
                •    Sample containers
                •    Shipping containers
                •    Access to the FORMS II Lite or  Scribe software for creating sample labels,  stickers, tags,  and
                    TR/COC Records
                •    Custody seals
                •    Sampling equipment such as bowls, augers, pumps, etc

                Sampling events may also require specific items such as:

                •    Cooler temperature blanks
                •    Trip blanks for VOA analysis
                •    Preservation supplies (e.g., ice or acid)
                •    Specially prepared sample vials (e.g., for SW-846 Method 5035A)
                •    Utensils or equipment in handling tissue samples requested by modified analysis

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                                                                               Chapter 2 - Pre-field Activities
       2.6.2   Procure Sample Containers

                The analytical protocol(s) to be used for sample analysis often requires the use of a particular type of
                sample container. The type of container also may depend on the sample  matrix  and analysis. It  is
                recommended that samplers use borosilicate glass containers,  which are inert to most materials, when
                sampling for pesticides and/or other organics.  Conventional polyethylene  is  recommended when
                sampling for metals because of the lower cost and absorption rate of metal ions.

                Using the wrong container may result in breakage, gathering of an insufficient volume needed to perform
                sample analysis, or the container material may interfere with  the analysis. Therefore, samplers should
                identify and use the correct sample containers for each sampling event.
                         Have extra containers readily available for each sampling event in case of breakage, loss, or
                         contamination.
                Containers procured for a sampling event are usually pre-cleaned and shipped ready for use from the
                manufacturer to the sampling site. Regardless of the type of container used, samplers must ensure that the
                containers have been analyzed or certified clean to levels below concern for the project. These containers
                must meet the USEPA container type specifications listed in Table 2-2.
                          Samplers should document the lot numbers for every lot of cleaned containers used for each
                          project and maintain corresponding certificates of analysis on file and available upon request.
                                     Table 2-2. Sample Container Type Specifications
Reference
Number
1
2
3
4
5
6
1
8
9
Container Type
40 mL amber glass vial, 24
mm neck finish.
1 L high density polyethylene,
cylinder-round bottle, 28 mm
neck finish.
8 oz short, wide mouth,
straight- sided, glass jar, 70
mm neck finish.
4 oz (120 mL) tall, wide
mouth, straight- sided, glass
jar, 48 mm neck finish.
1 L amber round glass bottle,
33 mm pour-out neck finish.
500 mL high density
polyethylene, cylinder-round
bottle, 28 mm neck finish.
Coring tool used as a transport
device (e.g., 5 g Sampler).
250 mL high density
polyethylene, cylinder-round
bottle, 28 mm neck finish.
1 qt polymer zip-top bag
Specifications
Closure
Polypropylene or phenolic, open-top
screw-cap, 15 cm opening, 24-400
size.
Polyethylene cap, ribbed, 28-410
size; F217 polyethylene liner.
Polypropylene or phenolic cap, 70-
400 size; 0.015 in. PTFE liner.
Polypropylene or phenolic cap, 48-
400 size; 0.015 in. PTFE liner.
Polypropylene or phenolic cap, 33-
430 size; 0.015 in. PTFE liner.
Polypropylene cap, ribbed, 28-410
size; F217 polyethylene liner.
Has built-in closing mechanism.
N/A
Has built-in closing mechanism.
Septum
24 mm disc of 0.005 in.
Porytetrafiuoroethylene
(PTFE) bonded to 0.120 in.
silicone for a total thickness
of 0.125 in.
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
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Chapter 2 - Pre-field Activities
                                    Table 2-2. Sample Container Type Specifications
Reference
Number
10
11
Container Type
Filter cassette used as
transport device
Heavy duty aluminum foil
Specifications
Closure
N/A
N/A
Septum
N/A
N/A
               The information contained in this table is also cross-referenced in the sample collection parameters
               discussed in Chapter 3. The container Reference Numbers are used in Tables 3-2 to 3-5 under the
               Containers column. For example, samples collected for low-level soil VOA  analysis using SW-846
               Method 503 5A may require the sampler to use pre-prepared, tared closed-system purge-and-trap vials
               with a preservative (refer to Appendix B).  Refer to the Regional QAPP and Appendix D for additional
               references.

       2.6.3   Procure Shipping Supplies

               Samples should be correctly packaged into the appropriate shipping containers to reduce the risk of
               breakage or leakage, and the shipping containers should be appropriately prepared for shipment. Before
               heading into the field, samplers should refer to the appropriate project plans to determine  the types of
               samples that will be taken during the sampling project so that samplers will have the proper packaging
               materials at the site for all pertinent sample container types and sample matrices.
        0)
Samplers should also make  sure to  obtain the appropriate shipping paperwork (e.g.,  shipping forms
required by the delivery service).

  The CLP strongly discourages the use of vermiculite and cat litter as sources for packing material.
  These materials interfere with labeling and documentation and are difficult to remove from sample
  containers and shipping containers.
2.7    Comply with Transportation and Shipping Requirements

        Samplers are expected to review the applicable project plans to be aware of all State, Federal, Department of
        Transportation (DOT), and International Air Transport Association (IATA) regulations governing environmental
        and hazardous sample packaging. The person who ships the samples is responsible for being in compliance with
        applicable packaging, labeling, and shipping requirements.

                 Samplers should request  and receive USDA soil permits for soil samples shipped from outside the
                 continental United States,  prior to shipping.

        Additional information can be obtained on Hazardous Materials Safety Program regulations from the DOT's
        Research and Special Programs Administration. Federal transportation regulations can be found in 49-CFR Parts
        100-185, and are available on the Internet at:
                                  http://www.phmsa.dot.qov/hazmat/reqs

2.8    Provide Shipment Notification

        Some Regions may require that samplers notify their RSCC (or designee) when samples are shipped, and some
        Regions allow samplers to contact SMO directly to provide shipment notification. It is recommended that samplers
        contact the RSCC of sample  origin to verify if such notification is necessary. If samplers are shipping samples
        after 5:00 PM ET, samplers must notify the RSCC (or designee) and SMO by 8:00 AM ET on the following
        business day.
16
                                                                            January 2011

-------
                                                                             Chapter 2 - Pre-field Activities
                 For Saturday delivery at the laboratory, samplers MUST notify the RSCC (or designee) and SMO as
                 soon as possible so that SMO will receive the delivery information by 3:00 PM ET on the Friday prior
                 to delivery.


2.9    Perform Readiness  Review/Dry Run

        A readiness review/dry run is a  test run of the proposed sampling event. This is a recommended practice since it
        gives samplers a chance to  check  all plans, documentation software (i.e., FORMS  II Lite  or Scribe), and
        equipment lists for accuracy  and completeness prior to  sampling activities. It also provides an opportunity to
        consult with sampling team members to make sure all the elements are in place and everyone understands their
        task before actually going out to the field. Sampling project managers should provide the readiness review or dry
        run dates and schedules to samplers so that samplers can prepare accordingly.
January 2011                                                                                          17

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18                                                                      January 2011

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                                                                                Chapter 3 - In-field Activities
3.0   IN-FIELD ACTIVITIES

        This chapter addresses the in-field activities a sampler will focus on during a
        sampling event  such as  determining the type of samples to be  collected;    At-a-Glance:
        collecting  the samples;  meeting volume, preservation,  and holding time    In-field Activities
        requirements; completing documentation; and packing and shipping samples.     ^

        When performing a  sampling event, the  sampler  is expected  to follow    ^   Complete documentation
        prescribed sampling techniques.  The sampler should also be aware of any    ^   Provide sample receipts
        special  sampling  considerations,   contamination   issues,  and  sample    ^   Procure shipping
        compositing  and mixing methods that could affect  their sampling efforts.    S   Pack and ship samples
        Please refer to Appendix D for more detailed information.                     ^^^^^^_^^^^^^_
                  Appropriate Regional guidance and procedures should be consulted for detailed sample collection,
                  preservation, handling and storing, equipment decontamination, and QA/QC procedures.
3.1    Collect Samples
        CLP RAS are generally used to analyze samples from Superfund sites. The matrices can be water, soil, sediment,
        filter, or wipe. Additional matrices requested under modified analysis may include oil, sludge, ash, biosolid or
        tissue.

        A CLP sample consists of all sample aliquots (portions), provided that the analyses are all requested from the same
        CLP analytical program:

        •   for each individual or set of analytical analyses
        •   from one station location
        •   for one sample matrix
        •   at one concentration level
        •   for one laboratory
        •   for one analytical program

        In some instances, a mixed-matrix sample may be collected which contains either a supernate (for a sediment/soil
        sample) or a precipitate  (for a water sample). In this event, samplers should consult their sampling plans and/or
        discuss the  required procedures with the RPM (or designee). In general, it is recommended that two individual
        samples be  collected by separating the aqueous layer from the solid/precipitate layer at the point of collection if
        possible. If the phases or layers cannot be separated effectively in the field at the point of collection, arrangements
        should  be  made  to separate  the layers  under controlled conditions  at the receiving laboratory. In this case,
        additional sample numbers will be needed for the  separate phases. They may be assigned two different sample IDs
        (e.g., Sample IDs ABC124 and ABC125 for Sample ID ABC123), along with a note in the field sample log or
        tracking system that the sample IDs are derived or related to the same sample ID, to ensure correct follow-up upon
        receipt of results from the laboratory.

       3.1.1    Determine Types  of Samples to be Collected

        Samplers may be required to take  several types of samples or sample aliquots during a sampling event. They
        should refer to their project plans to determine the types of samples or aliquots to be taken, the volumes needed of
        each sample or aliquot, and the preservation needed for each sample. For an explanation of the various sample
        types and the requirements for collecting and submitting each particular type, refer to Table 3-1.
January2011                                                                                           19

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 Chapter 3 - In-field Activities
                             Table 3-1. Sample Types and CLP Submission Requirements
 Sample Type
           Purpose
                                                                    Collection1
                                                         CLP Sample Number
Field Duplicate
To check reproducibility of
laboratory and field procedures.
To indicate non-homogeneity.
Collect from areas that are known or suspected to be
contaminated.
Collect one sample per week or 10% (Regions may
vary) of all field samples per matrix, whichever is
greater.
Assign two separate (unique) CLP
Sample numbers (i.e., one number to the
field sample and one to the duplicate).
Submit blind to the laboratory.
Field Blank
                 To check cross-contamination
                 during sample collection,
                 preservation, and shipment, as
                 well as in the laboratory. Also to
                 check sample containers and
                 preservatives.
                                 Collect for each group of samples of similar matrix
                                 with the frequency specified in the QAPP and
                                 Sampling Plan.
                                 Organics - Use water (demonstrated to be free of the
                                 contaminants of concern).
                                 Inorganics - Use metal-free (deionized or distilled)
                                 water or a single clean wipe or filter.
                                                 Assign separate CLP Sample numbers to
                                                 the field blanks.
Temperature
Blank
To provide an accurate
measurement of field sample
upon arrival to the laboratory.
Also to establish whether the
temperature range has been
maintained while in transit.
Collect for each shipping container with the
frequency specified in the QAPP and Sampling Plan.
Shipped together with samples from the
field to the laboratory.
Trip Blank
(Volatile
Organic
Analysis Only)
To check contamination of VOA
samples during handling, storage,
and shipment from field to
laboratory.
Prior to going into the field, prepare and seal one trip
blank sample per shipment per matrix. Trip blanks
should be matched with respect to matrix and
volume of the preservatives used. Before going into
the field prepare trip blank samples with the same
laboratory grade methanol and sodium bisulfate
solution or reagent water used for field sampling.
Carry each through the same sampling and handling
protocols used for field samples. Aqueous trip blank
samples should be prepared using water
demonstrated to be free of the contaminants of
concern (deionized water is appropriate).
Place one trip blank sample for each matrix in each
cooler used to ship VOA samples.
Assign separate CLP Sample numbers to
the trip blanks.
Equipment
Blank or
Rinsate Blank
To check field decontamination
procedures.
Collect when sampling equipment is decontaminated
and reused in the field or when a sample collection
vessel (bailer or beaker) will be used. Use blank
water (water demonstrated to be organic-free,
deionized or distilled for inorganics) and rinse water
into the sample containers.
Assign separate CLP Sample numbers to
the equipment blanks.
Matrix Spike
(MS) and
Matrix Spike
Duplicate
(MSD) (Organic
Analysis Only)
To check accuracy and precision
of organic analyses in specific
sample matrices.
Collect from areas that are known or suspected to be
contaminated. For smaller sampling events (i.e., 20
samples or less), MS/MSD additional volume should
be collected in the first round of sampling and
included in the first shipment of samples to the
laboratory.
Collect triple volume2 for aqueous samples and soil
VOA samples designated for MS/MSD analyses.
Collect double volume for soil samples requiring
SVOA, Pesticide, and/or Aroclor analysis and
MS/MSD. See Table 3-2 and Appendix B for VOA
collection volumes.
Assign the same CLP Sample number to
the field sample and the extra volume for
MS/MSD.
Identify the sample designated for
MS/MSD on the TR/COC record.
Matrix Spike
(MS) and
Duplicate
(Inorganic
Analysis Only)
To check accuracy and precision
of inorganic analyses in specific
sample matrices.
Collect from areas that are known or suspected to be
contaminated. For smaller sampling events (i.e., 20
samples or less), Matrix Spike and Duplicates should
be collected in the first round of sampling and
included in the first shipment of samples to the
laboratory.
Additional sample volume may be required for
inorganic analysis.3
Assign the same CLP Sample number to
the field sample and extra volume (if
collected).
Identify the sample(s) designated for
Matrix Spike and Duplicates on the
TR/COC record.
 20
                                                                                                     January 2011

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                                                                                         Chapter 3 - In-field Activities
                          Table 3-1. Sample Types and CLP Submission Requirements
  Sample Type
          Purpose
                                                            Collection1
                                                  CLP Sample Number
 PE Samples
Specially-prepared QC samples
used to evaluate a laboratory's
analytical proficiency.
The PE samples contain analytes with concentrations
unknown to the laboratory. Designated Regional or
authorized personnel (depending on Regional policy)
arrange for Case-specific CLP PE samples to be
prepared and shipped by the QATS contractor. The
PE samples can be shipped to the site, or shipped per
Regional direction. QATS provides the appropriate
preparation instructions and chain-of-custody
materials.
Samplers must order PE samples and
ship them to the laboratory if required by
the Region.
 Consult Regional or Project Manager Guidance for field QC sample frequencies; laboratory QC sample frequencies are generally fixed in the laboratory
 subcontracts or specified in analytical methods.
2 An aqueous sample for SVGA analysis would require the field sampler to collect at least 2 L of field samples and at least 2 L each for the MS and MSD
 samples for a total volume of 6 L. If Pesticide or Aroclor MS/MSD analyses are required for the same sample, an additional 6 L must be collected for each
 analysis method. Collect additional volume for MS/MSD samples to allow for sufficient volume for these analyses in the event sample volume is lost as a
 result of samples breaking, leaking, or laboratory accidents.
3 Double volume should be sent for inorganic aqueous MS and Duplicate samples to allow for sufficient volume for these analyses in the event sample
 volume is lost as a result of samples breaking, leaking, re-extraction/redigestion, reanalysis, or laboratory accidents.  Additional soil volume is not necessary
 for inorganic samples.

                   3.1.1.1   Collect Field QC Samples

                              Field QC samples are designed to assess variability of the media being  sampled and to detect
                              contamination  and sampling error in the field. The types  of field QC  samples that are
                              generally collected  include field duplicates and field blanks (such as equipment, trip, or rinse
                             blanks). Generally,  field duplicate samples should  remain "blind" to the laboratory (i.e., they
                              should have separate CLP Sample  numbers).

                   3.1.1.2  Collect Laboratory  QC Samples

                              A laboratory QC  sample  is  an additional  analysis of  a field sample, as required by the
                              laboratory's contract. There are three types of laboratory QC samples:

                              •   MS (for organic and inorganic samples)
                              •   MSD  (for organic samples only)
                              •   Duplicates (for inorganic samples only)
                                      Samplers should obtain Regional guidance regarding the collection of laboratory
                                      QC  samples (especially for organics analyses). These are not required for wipes
                                      and air filter samples.
                              Samplers should select one sample per matrix per 20 samples as a "laboratory QC" sample.
                              Designated organic laboratory QC samples should be noted on the Organic TR/COC record.
                              Designated inorganic  laboratory  QC  samples  should be noted on the Inorganic  TR/COC
                              record. The sample(s)  designated for laboratory QC  should be noted  in both "QC Type"
                              column and the "Sample(s) to be used for laboratory QC" fields on the Organic and Inorganic
                              TR/COC records.

                              The sampler should select a field sample as the  laboratory QC sample. QC samples should be
                              sent in the same cooler as the field samples when possible. If the sampler fails to designate a
                              laboratory QC, this may cause a delay in the analyses of the samples.
                                      In  the event  of  multiple  sample  shipments during  a  sampling  event,  it  is
                                      recommended  that the sampler submit laboratory QC samples in the first sample
                                      shipment,  and as necessary in subsequent shipments to meet laboratory contract
                                      requirements.
  January 2011
                                                                                                    21

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Chapter 3 - In-field Activities
       3.1.2   Meet Volume, Preservation, and Holding Time Requirements

                Samplers  should refer to their project plans to obtain the specific sample volumes to be collected, the
                preservation needed for those samples, and the technical holding times under which they must submit
                samples to the scheduled  CLP  laboratory. Sample  collection parameters  (including  sample volumes,
                preservatives, and technical holding times) for organic collection and analysis are listed in Tables 3-2 and
                3-3. Sample collection parameters for inorganic analysis and collection are listed in Table 3-4 and 3-5.

                3.1.2.1  Collect Sample Volume

                         Collecting  sufficient  sample  volume is critical. There  must be sufficient physical  sample
                         volume for the analysis of all required parameters  and completion of all QC determinations.
                         The type of analytical procedure(s) to be performed will often dictate the sample volume to
                         collect. For example,  each water sample collected for VOA analysis by CLP SOW SOM01.X
                         requires a minimum of three vials, each filled completely to a 40 mL capacity. See Appendix
                         C for information regarding the  collection of VOAs in water. When sampling for VOAs in
                         soils,  samplers  must  use SW-846 Method 503 5A guidelines included in Appendix B. It is
                         extremely important that samplers refer to their specific project plans to identify and collect
                         the correct sample volume during each sampling event.

                                 If  a  modified  analysis  requesting tissue samples  required  processing  or
                                 homogenizing, it should be performed at  a sample processing facility under clean
                                 room condition  to  reduce  potential  contamination. Tissue samples  should be
                                 packed and  cooled on ice  immediately. Tissue samples should never be sent on
                                 Friday for Monday delivery.

                3.1.2.2  Preserve Samples

                         Degradation of some contaminants may occur naturally  (e.g., VOAs).  The sampler  must
                         chemically preserve  some water samples for certain analytes before shipping them to the
                         laboratory.  Any visible reaction between the sample and added chemical preservative should
                         be noted in the field record.

                         The sampler should preserve  and immediately cool all  water samples to 4°C  (±2°C) upon
                         collection and samples should remain cooled until the time of analysis (do not freeze water
                         samples). Preservation techniques vary among Regions so the sampler should obtain Region-
                         specific instructions and review the appropriate project plans and SOPs. See Appendix C for
                         information regarding the collection of VOAs in water.

                3.1.2.3  Ship within Holding Times

                         Samplers should ship samples to scheduled CLP  laboratories as soon as possible after
                         collection. Daily shipment of samples to  CLP laboratories is preferred whenever possible. If
                         samples cannot be shipped on a daily basis, they must be properly preserved and maintained to
                         meet CLP-specified temperatures, holding times, and custody requirements.

                         The technical holding times are the maximum time allowed between a sample collection and
                         the completion of the sample  extraction and/or analysis. In  contrast, contractual holding times
                         are the maximum lengths of time  that the CLP  laboratory can hold the sample prior to
                         extraction and/or analysis. The contractual holding time is the elapsed time expressed in days
                         from the date of receipt of the  sample by the laboratory until the date of its analysis, as
                         described in the appropriate CLP SOW. Contractual holding times are generally  set to be two
                         days less than the technical holding times to allow for sample packing and shipping.

                                 If samplers are shipping samples after 5:00 PM ET, they must notify the RSCC (or
                                 designee) and SMO by 8:00 AM ET on the following business day. When making
                                 a Saturday delivery,  samplers must notify the  RSCC (or designee) and SMO as
                                 soon as possible so that SMO will receive the delivery information by 3:00 PM ET
                                 on the Friday prior to delivery.


22                                                                                           January 2011

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                                                                                                                                            Chapter 3 - In-field Activities
                                         Table 3-2. Sample Collection Requirements for CLP SOW SOM01.X (VOAs)
Matrix

Water
Soil/
Sediment
Sample
Type

Samples
Only
Samples
with SIM
Samples
with
MS/MSD
Samples
Only
Samples
with
MS/MSD
Samples
Only
Samples
with
MS/MSD
Samples
Only
Samples
with
MS/MSD
Container Type1

40 mL amber glass
vial, 24 mm neck
finish. See Table 2-
2, Reference Number
1.
OPTION 1
Closed-system 40 mL
amber glass vial
containing magnetic
stirrer, 24 mm neck
finish.
See Table 2-2,
Reference Number 1 .
OPTION 2
Closed-system 40 mL
amber glass vial
containing magnetic
stirrer, 24 mm neck
finish and 5 mL
water.
See Table 2-2,
Reference Number 1 .
OPTION 3
Coring tool used as a
transport device. See
Table 2-2, Reference
Number?.
Minimum Number of Containers
Needed
with
Water
-
-
-
-
-
2
6
-
-
Dry
-
-
-
3
9
1
1
3
9
%
Moisture
-
-
-
1
1
1
1
1
1
TOTAL
3
5
9
4
10
4
8
4
10
Minimum
Volume/Mass2

Fill to capacity
5g
5g
5g
Important Notes

Containers/vials must
be filled to capacity
with no headspace or
air bubbles.
Refer to Appendix C
for samples requiring
QC analyses.
Place samples on side
prior to being iced.
Refer to Appendix B
for samples requiring
QC analyses.
Place samples on side
prior to being iced.
Refer to Appendix B
for samples requiring
QC analyses.
Refer to Appendix B
for samples requiring
QC analysis.
Place samples on side
prior to being iced.
Preservative3

Preserve to a pH of
2 with HC1 and
cool to 4°C (±2°C)
immediately after
collection. DO
NOT FREEZE
water samples.
Frozen to (-D7°C
to-Dl5°C)
OR
Iced to 4° (±2°C).
Frozen to (-D7°C
to-Dl5°C)
OR
Iced to 4° (±2°C).
Frozen to (-D7°C
to-Dl5°C)
OR
Iced to 4° (±2°C).
Technical Holding
Time4

14 days
14 days
OR
48 hours
(unpreserved)5
14 days
OR
48 hours
(unpreserved)5
14 days
OR
48 hours
(unpreserved)5
   Vials for soil analysis are typically pre-labeled and tared vials for water analysis are not pre-labeled or tared.
   Minimum volume/mass to be collected in order to ensure sample analysis can be performed. Collect additional volume for MS/MSD samples to allow for sufficient volume for these
   analyses in the event sample volume is lost as a result of samples breaking, leaking, or laboratory accidents.
   Check Regional guidance regarding use of acid as a preservative of samples that may contain carbonates, residual chlorine, and other oxidants.
   Technical holding time is calculated from the time of sample  collection to sample extraction, and determined as 14 days for preserved (frozen or iced) samples and 48 hours for non-
   preserved (iced) samples. Sample extracts are to be analyzed within 40 days of extraction. It is recommended that samplers ship samples to the laboratory on the same day that they are
   collected, or as soon as possible thereafter.
   Unpreserved soil samples can be frozen or iced at the time of receipt by the laboratory to increase holding time.
January 2011
23

-------
  Chapter 3 - In-field Activities
                     Table 3-3. Sample Collection Requirements for CLP SOW SOM01.X (SVGAs, Pesticides and Aroclors)
Analysis
SVGAs
SVGA SIM
Matrix
Water3
Soil/
Sediment4
Water3
Soil/
Sediment4
Sample Type
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Container Type
1 L amber round glass bottle, 33 mm pour-
out neck finish. See Table 2-2, Reference
NumberS.
1 L amber round glass bottle, 33 mm pour-
out neck finish. See Table 2-2, Reference
Number 5.
One 8 oz short, wide mouth, straight-sided,
glass jar, 70 mm neck finish or two 4 oz tall,
wide mouth, straight-sided, glass jar, 48 mm
neck finish. See Table 2-2, Reference
Numbers 3 and 4.
Two 8 oz short, wide mouth, straight- sided,
glass jars, 70 mm neck finish or 4 oz tall,
wide mouth, straight- sided, glass jar, 48 mm
neck finish. See Table 2-2, Reference
Numbers 3 and 4.
1 L amber round glass bottle, 33 mm pour-
out neck finish. See Table 2-2, Reference
NumberS.
1 L amber round glass bottle, 33 mm pour-
out neck finish. See Table 2-2, Reference
NumberS.
One 8 oz short, wide mouth, straight-sided,
glass jar, 70 mm neck finish or two 4 oz tall,
wide mouth, straight-sided, glass jar, 48 mm
neck finish. See Table 2-2, Reference
Numbers 3 and 4.
Two 8 oz short, wide mouth, straight- sided,
glass jars, 70 mm neck finish or four 4 oz
tall, wide mouth, straight-sided, glass jar, 48
mm neck finish. See Table 2-2, Reference
Numbers 3 and 4.
Minimum
Volume/Mass1
2L
6L
150 grams
300 grams
2L
10L
150 grams
300 grams
Important Notes
If amber containers are
not available, the samples
should be protected from
light.

If amber containers are
not available, the samples
should be protected from
light.

Preservative/
Collection
Cool all samples to
4°C (±2°C)
immediately after
collection. DO NOT
FREEZE water
samples.
Cool all samples to
4°C (±2°C)
immediately after
collection.
Cool all samples to
4°C (±2°C)
immediately after
collection. DO NOT
FREEZE water
samples.
Cool all samples to
4°C (±2°C)
immediately after
collection.
Technical
Holding
Time2
7 days
14 days
7 days
14 days
24
January 2011

-------
                                                                                                                                           Chapter 3 - In-field Activities
                   Table 3-3. Sample Collection Requirements for CLP SOW SOM01.X (SVGAs, Pesticides and Aroclors) (Continued)
Analysis
Pesticides
Aroclors
Matrix
Water3
Soil/
Sediment4
Water3
Soil/
Sediment4
Sample Type
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Samples Only
Samples with
MS/MSD
Container Type
1 L amber round glass bottle, 33 mm pour-out neck
finish. See Table 2-2, Reference Number 5.
1 L amber round glass bottle, 33 mm pour-out neck
finish. See Table 2-2, Reference Number 5.
One 8 oz short, wide mouth, straight-sided, glass jar,
70 mm neck finish or four 4 oz tall, wide mouth,
straight-sided, glass jar, 48 mm neck finish. See
Table 2-2, Reference Numbers 3 and 4.
Two 8 oz short, wide mouth, straight-sided, glass
jars, 70 mm neck finish or four 4 oz tall, wide
mouth, straight- sided, glass jar, 48 mm neck finish.
See Table 2-2, Reference Numbers 3 and 4.
1 L amber round glass bottle, 33 mm pour-out neck
finish. See Table 2-2, Reference Number 5.
1 L amber round glass bottle, 33 mm pour-out neck
finish. See Table 2-2, Reference Number 5.
One 8 oz short, wide mouth, straight-sided, glass jar,
70 mm neck finish or 4 oz tall, wide mouth, straight-
sided, glass jar, 48 mm neck finish. See Table 2-2,
Reference Numbers 3 and 4.
Two 8 oz short, wide mouth, straight-sided, glass
jars, 70 mm neck finish or four 4 oz tall, wide mouth,
straight- sided, glass jar, 48 mm neck finish. See
Table 2-2, Reference Numbers 3 and 4.
Minimum
Volume/Mass1
2L
6L
150 grams
300 grams
2L
6L
150 grams
300 grams
Important Notes
If amber containers
are not available,
the samples should
be protected from
light.

If amber containers
are not available,
the samples should
be protected from
light.

Preservative/
Collection
Cool all samples to
4°C (±2°C)
immediately after
collection. DO NOT
FREEZE water
samples.
Cool all samples to
4°C (±2°C)
immediately after
collection.
Cool all samples to
4°C (±2°C)
immediately after
collection. DO NOT
FREEZE water
samples.
Cool all samples to
4°C (±2°C)
immediately after
collection.
Technical Holding
Time2
7 days
14 days
7 days
14 days
Notes
   Minimum volume/mass to be collected in order to ensure sample analysis can be performed.
   This technical holding time is calculated from the time of sample collection to sample extraction. Sample extracts are to be analyzed within 40 days of extraction. It is recommended that
   samplers ship samples to the laboratory on the same day that they are collected, or as soon as possible thereafter.
   An aqueous sample for SVOA analysis would require the field sampler to collect at least 2 L of field samples and at least 2 L each for the MS andMSD samples for a total volume of 6 L. If
   Pesticide or Aroclor MS/MSD analyses are required for the same sample, an additional 6 L must be collected for each analysis method. Collect additional volume for MS/MSD samples to
   allow for sufficient volume for these analyses in the event sample volume is lost as a result of samples breaking, leaking, or laboratory accidents.
   If one or two extractable analyses are required for soil/sediment, only a single 8 oz. jar is required. If three extractable analyses are required, two 8 oz. jars are required. The number of jars
   should be doubled if MS/MSD is required.
January 2011
25

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  Chapter 3 - In-field Activities
                                                    Table 3-4. Sample Collection Requirements for CLP SOW ISM01.X

Analysis
Metals/ICP-AES,
Mptnk/TPP MS nnH/or
Mercury by CVAA


Metals/ICP-AES6



Cyanide/
Spectrophotometric
Determination




Matrix
Water

Soil/
Sediment5
Wipe
Filter


Water



Soil/
Sediment5


Sample Type
Samples Only
Samples with
MS/Duplicate

Samples Only
Samples with
MS/Duplicate



Samples Only
Sample with
MS/Duplicate



Samples Only
Samples with
MS/Duplicate

Container Type
1 L high density polyethylene, cylinder-round
bottle, 28 mm neck finish. See Table 2-2,
Reference Number 2.

One 8 oz short, wide mouth, straight-sided,
glass jar, 70 mm neck finish. See Table 2-2,
Reference Number 3.
1 qt polymer zip-top bag. See Table 2-2,
Reference Number 9.
Filter cassette (25 or 37 mm) as transport
device. See Table 2-2, Reference Number 10.


1 L high density polyethylene, cylinder-round
bottle, 28 mm neck finish. See Table 2-2,
Reference Number 2.


One 8 oz short, wide mouth, straight-sided,
glass jar, 70 mm neck finish. See Table 2-2,
Reference Number 3.


Volume/
Mass1
1L
2L

Fill to
capacity
N/A
N/A

1L

2L


Fill to
capacity


Important
Notes
DO NOT
FREEZE
water
samples.







DO NOT
FREEZE
samples.





Preservative/ Collection2
Acidify to pH < 2 with
HNO3 and cool to 4°C
(±2°C) immediately after
collection.4

Cool to 4°C (±2°C)
immediately after
collection.
Store at room temperature.
Store at room temperature.
To neutralize residual
chlorine, add 0.6 g
of sample collected,
immediately upon
collection
Add NaOH until pH>12
and cool to 4°C (±2°C)
immediately after
collection.
Cool to 4°C (±2°C)
immediately after
collection.


Technical
Holding Time3
6 months for all
metals except
Mercury (28
days)


6 months
6 months
6 months


14 days



14 days

   Minimum volume/mass to be collected in order to ensure sample analysis can be performed.
   Check Regional guidance regarding use of acid as a preservative of samples that may contain carbonates, residual chlorine, and other oxidants.
   This technical holding time is calculated from the time of sample collection to sample extraction. Sample extracts are to be analyzed within 40 days of extraction.
   Water samples collected for total metal and filtered metal analyses from the same sampling location must be assigned separate (unique) CLP Sample Numbers.
   Only one 8 oz. jar is needed for soil/sediment when all metals (including mercury) and cyanide analyses are required for soil/sediment samples.
   Filter method is intended for 25 mm or 37 mm mixed cellulose ester (MCE) filters in cassettes. Wipe materials have varied from laboratory tissues (e.g., Kimwipes®) to pre-moistened "baby
   wipes" from the nearest store.
   Samplers must test for sulfide and oxidizing agents (e.g., chlorine) in aqueous samples in the field upon collection. Please refer to the SAP and Appendix C for guidance. Sulfides adversely
   affect the analytical procedure. The following can be done to test for and neutralize sulfides. Place a drop of the sample on lead acetate test paper to detect the presence of sulfides. If sulfides are
   present, treat 25 mL more of the sample than that required for the cyanide determination with powdered cadmium carbonate or lead carbonate. Yellow cadmium sulfide or black lead sulfide
   precipitates if the sample contains sulfide. Repeat this operation until a drop of the treated sample solution does not darken the lead acetate test paper. Filter the  solution through a dry filter paper
   into a dry beaker, and from the filtrate measure the sample to be used for analysis. Avoid a large excess of cadmium carbonate and a long contact time in order to minimize a loss by
   complication or occlusion of cyanide on the precipitated material. Sulfide removal should be performed in the field, if practical, prior to pH adjustment with NaOH.
26
January 2011

-------
                                                                             Chapter 3 - In-field Activities
3.2    Complete Documentation

        Samplers must complete all documentation, including the recording of the CLP Sample Number on the sample
        container or bottle, sample labels, and chain-of-custody seals (as appropriate), the TR/COC record, and the field
        operations records (as necessary).

        Samplers should use the FORMS II Lite or Scribe software to create and print sample labels and the TR/COC
        record. Samplers can create and print out two copies of a sample label and attach one to the sample container or
        bottle, and place the other on the sample tag that may be attached to the sample container or bottle.

        Samplers are expected to review their project plans to determine what documentation they are expected to include
        during a sampling event. It is highly recommended that samplers provide documentation, even if the Region does
        not require it.

                Under no  circumstances should the site name  appear on  any documentation being  sent to the
                laboratory, unless the laboratory is  a Regional USEPA laboratory.  Then the Region copy of the
                TR/COC record shall be sent to the USEPA laboratory.
       An example of a packaged sample is shown in Figure 3-1. A description of each type of documentation and
       instructions for accurate completion are included in the following sections.
                                 Custody.
                                  Seal
                               Sample
                              Container
Clear plastic
   bag
                                                                 Sample
                                                                 Sample
                                                                   Tag
   Figure 3-1. Packaged Sample with Identification and Chain-of-Custody Documentation (Excluding
                                           TR/COC Record)

       3.2.1   Identify a  Sample with a  CLP Sample Number and  SMO-assigned CLP
               Case Number
               The analysis method, CLP Sample number, and SMO-assigned CLP Case number must be recorded on
               each sample taken during a sampling event (see Section 1.4.3). Samplers record these numbers on the
               sample tag, bottle or container label using permanent ink. The numbers must also be recorded on the
               sample tag, if required.

                      Filtered metal samples and total metal samples taken from the same sampling location cannot
                      have the same CLP  Sample Number because two different sets of data will be generated.
                      Water samples collected for total metal and filtered metal analyses from the same sampling
                      location must be assigned separate (unique) CLP Sample numbers.
       3.2.2   Complete TR/COC Records

               A Traffic Report is used as physical evidence of sample custody and as a permanent record for each
               sample collected. A chain-of-custody record documents the exchange and transportation of samples from
               the field to the laboratory.

               ASB requires samplers to use the FORMS II Lite or Scribe software to create documentation for all CLP
               sampling efforts. For assistance with obtaining or using the FORMS II Lite software, please contact the
               FORMS II Lite Help Desk at 703-818-4200 from 9:00 AM - 5:00 PM ET.  For assistance with obtaining
January 2011
                                         27

-------
Chapter 3 - In-field Activities
               or using the Scribe software, please contact ERT Software Support Help Desk at 800-999-6990 from 9:00
               AM-5:OOPMET.

               To  meet CLP sample documentation and chain-of-custody requirements, the sampler must attach a
               separate TR/COC record to each cooler they ship. The TR/COC record must document each sample
               within the cooler. TPJCOC records should be separated and shipped in the coolers with the samples listed
               on them. Do  not ship samples in a cooler without the corresponding TPJCOC record. This practice
               maintains the chain-of-custody for all samples in case of incorrect shipment.

               If more than one TPJCOC record is used for the samples within one cooler, all of the records must have
               complete header information and original signatures. Samplers are responsible for the care and custody of
               samples from the time of collection to the time of shipment to the laboratories for analysis. A sample is
               considered under custody if:

               •   It is in possession or in view after being in possession
               •   It was in possession and then secured or sealed to prevent tampering
               •   It was in possession when placed in a secured area

               Each time the custody of samples is turned over to another person, the TPJCOC record must be signed off
               by the former custodian  and accepted by the new custodian. Samplers are, therefore, responsible  for
               properly completing any  forms or other Region-required documentation used to establish the chain-of-
               custody for each sample during a sampling event.

               3.2.2.1  Complete a TR/COC Record Using the  FORMS II  Lite Software

                         Once the sampler inputs sample collection information into FORMS II Lite, a TR/COC record
                         will be generated electronically. The software automatically displays only the information to
                        be entered by the sampler. FORMS II Lite then generates a laboratory and a Regional copy of
                         the TR/COC record (see Figures 3-2 through 3-5). The sampler can print out multiple copies
                         of the TR/COC record as necessary. The sampler must sign and submit original copies of the
                         TR/COC record as appropriate.

                         An electronic  TR/COC  record created using the FORMS II  Lite  software contains basic
                         header  information;  however,  the sampler can  also  include some  additional detailed
                         information. For example, not only is the sample  matrix listed on the electronic TR/COC
                         record, but the name of the sampler taking the sample can also be  entered. Samplers should
                         note that certain information will not appear  on the electronic TR/COC record (e.g.,  matrix
                         and preservative descriptions).

               3.2.2.2  Complete a COC Record Using the Scribe Software

                         Once the sampler inputs sample collection information into Scribe, a Chain-of-Custody (COC)
                         record  will be generated electronically.  The  software automatically displays only  the
                         information to be entered by the sampler. Scribe then generates a laboratory and a Regional
                         copy of the COC record (see Figures 3-6 through  3-9).  The sampler can print out multiple
                         copies of the COC record as necessary.  The sampler must sign and submit original copies of
                        the COC record as appropriate.

                         An electronic COC record created  using  the  Scribe software contains basic header
                         information; however, the sampler can also include  some additional detailed information. For
                         example, not only is the  sample matrix listed  on the electronic  COC record, but the name of
                        the  sampler taking the  sample  can also be entered.  Samplers  should note  that certain
                         information will not appear on the electronic  COC record (e.g., matrix and  preservative
                         descriptions).

               3.2.2.3  Indicate Modified Analysis on  FORMS II Lite TR/COC Records

                         When completing a TR/COC record using FORMS II Lite, the sampler should identify any
                         samples that will be analyzed using a CLP Modified Analysis. Samplers should indicate use of
                         a Modified Analysis by creating a new analysis within the FORMS II Lite Wizard or through
                         the  FORMS  II  Lite  reference  tables. This newly-created  analysis  should  contain  the
                         Modification Reference Number within the name assigned to the analysis. For example, if a
                         Region submits  a  Modified Analysis  for an additional  analyte, and SMO  assigns  the
                         Modification Reference Number 1301.0, the FORMS II Lite analysis could be named "VOA

28                                                                                         January 2011

-------
                                                                              Chapter 3 - In-field Activities
                         by M.A. 1301.0." The associated abbreviation for this analysis could be "VOA M.A." If you
                         have any questions regarding identification of Modified Analysis using FORMS II Lite, please
                         contact the FORMS II Lite Help Desk at 703-818-4200 from 9:00 AM - 5:00 PM ET.

               3.2.2.4  Indicate Modified Analysis on Scribe COC Records

                         When completing a COC record using Scribe, the sampler should identify any samples that
                         will be analyzed using a CLP Modified Analysis. Samplers should indicate use of a Modified
                         Analysis (MA) by creating a new analysis within the Scribe Analyses table or at the time of
                         entering the  Analyses  for the  sample.  This  newly-created analysis  should contain the
                         Modification Reference Number  within the name assigned to the analysis. For example, if a
                         Region submits a MA for an additional analyte, and SMO assigns the Modification Reference
                         Number 1301.0, the  Scribe Analyses could be named "CLP VOA by  M.A. 1301.0."  The
                         associated abbreviation for this analysis  could be "VOA M.A." If you have any questions
                         regarding identification of Modified Analysis using Scribe,  please contact the ERT Software
                         Support Help Desk at 800-999-6990.

               3.2.2.5  Make Manual Edits to Printed  FORMS II Lite TR/COC  Records

                         If a FORMS II Lite TR/COC Record has  been printed and deletions or edits need to be made
                         by the sampler, the following procedures must be followed:

                         •  If corrections occur after shipment, adhere to Region-specific procedures and guidelines
                            on handling hard copy TR/COC records.
                         •  If making a deletion,  manually cross  out the information  to be disregarded from the
                            TR/COC record, initial and date the deletion.
                         •  If making an addition, enter the new information and initial,  sign and  date the newly
                            added information.

                                All modifications made on a printed TR/COC record must be initialed and dated.


               3.2.2.6  Make Manual Edits to Printed  Scribe COC Records

                         If a Scribe COC Record has been printed and deletions or edits need to be made by the
                         sampler, the following procedures must be followed:

                         •  If corrections occur after shipment, adhere to Region-specific procedures and guidelines
                            on handling hard copy COC records.
                         •  If making a deletion, correct the deletion in Scribe and  reprint the COC record. Discard
                            the original.
                         •  If making an addition, enter  the new information in Scribe and reprint the COC record.
                            Discard the original.
January 2011                                                                                         29

-------
Chapter 3 - In-field Activities
<** I^C]M\ USEPA Contract Laboratory Program
^Ky -dii.r^ Organic Traffic Report & Chain of Custody Record
Data Shipped;
Carrier Name;
Airbill;
Shipped to:
ORGANIC
SAMPLE No.
1 1/9/2 QG9


1234 Smith Drive
(123) 456-7890

Chain of Custody Record
Relinquished By (Date / Time)
1
2.
3.
4
Sampler
Signature;
Received By (Date / Time)




Case No: 39400
DAS No; DAS90QQ
SDG No:
L
For Lab Use Only
Lab Contract No:
Unit Price:
Transfer To:
Lab Contract No:
UnR Price:
MATRIX' CONG,' ANALYSES' TAG No./ STATION SAMPLE COLLECT 1 NORGANIC FOR LAB USE ONLY
SAMPLER TYPE TURNAROUND PRESERVATIVE/ Bottles LOCATION DATETOME SAM PLE No Sample Condition On Receipt
                  C3TK2



                  C3TK4


                  C3TK5


                  C3TK6



                  C3TK7
Surface Water/
BOBBY
SAMPLER

Surface Water/
DAN SAMPLER
Surface Waler/
DAN SAMPLER
Surface Water/
JOHN SAMPLER
Surface Water/
JOHN SAMPLER
                                Surface Water/
                                JOHN SAMPLER
                 /G
  1310.0 (21), BNA  6-2119002, 6-2119003,
(14), CLP ARO (14). 6-2119004 (ice Only).
CLP P6ST(14). VOA 6-2119005 (Ice Only).
       (14)        6-2119006,6-2119008(6)
BNA{14), CLP ARO 6-2119010, 6-2119011,
(14), CLP P6ST (14). 6-2119012 (Ice Only),
     VOA(14)      6-2119013 (Ice Only),
                  6-2119014(5)
    1310,0(21)     6-2119026(1)
 BNA (14), CLP ARO
(14), CLP PEST (14).
     VOA (14)
  1310.0(21). BNA
 (14), CLP ARO (14).
CLP PEST (14),  VOA
       (14)
  1310.0(21). BNA
 (14). CLP ARO (14),
CLP PEST (14),  VOA
       (14)
6-2119027 (lee Only),
6-2119028 (Ice Only) (5)

6-2119033. 6-2119034,
6-2119035 (Ice Only).
6-2119036 (Ice Only),
6-2119037, 6-2119039(6)
6-2119041, 6-2119042,
6-2119043 (Ice Only),
6-2119044 (Ice Only).
6-2119045. 6-2119047(6)
                                                                                                 LOCATION ONE
                                                                                                 LOCATION TWO
                                                                 LOCATION FIVE
                                                                                                  LOCATION SIX
                                                                                                                    S: 11/6/2009
                                                                                                                    S: 11/9/2009
                                                                LOCATION THREE    S:  11/9/2009
                                                                 LOCATION FOUR    S:  11/9/2009
                                                                                    S:  11/9/2009
                                                                                                                    S: 11/9/2009
14:57     MC3TK1



8:13     MC3TK2



8:14     MC3TK4


8:14


8:14     MC3TK6



8:14     MC3TK7
Shipment for Case
CompieteTN
Analysis Key:
1310.0= VOA by MA 13
Sampfe(s) to be used for laboratory QC:
C3TK1
Additional Samptet Signatures);
Cooler Temperature
Upon Receipt:
Concentration: L =Low.M = Low/Meaium . H = High Type/Designate: Composite *C. Grab = G
Chain of Custody Seal Number:
Custody Seal Intact? 	 Shipment teed?
10.0. BNA= CLPTCL Semi vol a tries, CLP ARO = CLP TCL PCB (Arocicrs), CLP PEST = CLP TCL Pesticides, VOA = CLP TCL Volatile 5
              TR Number:      3-043013577-050310-0004                                                              I
              PR provides preliminary results. Requests for preliminary results vUll increase analytical costs.
              Send Copy to: Sample Management Office. Ann: HeaSher Bauer. CSC, 15000 Conference Center Dr.. Chantilly. VA 20151-3819: Ptione 703/818-4200: Fax
              703/818-4602
                                                                                                                               F2V5.1. 047 paoe 1 of 1
                               Figure 3-2. FORMS II Lite Organic Traffic Report & Chain of Custody Record (Laboratory Copy)
30
                                                                                                                                       January 2011

-------
                                                                                                                             Chapter 3 - In-field Activities
SEPA

Date Shipped:
Carrier Name:
nit DIM:
snipped to:
INORGANIC
SAMPLE No.
MC3TK1
MC3TK2
MC3TK4
MC3TKS
MC3TK7


USEPA Contract Laboratory Program
Inorganic Traffic Report & Chain of Custody Record
1 1/9/2009
FedEx
123456789937654321
Inorganic Laboratory
1234 Wats en Drive
AnywhereNC 123456
(123)456-7832
MATRIX! CONCf
SAMPLER TYPE
Surface Water/ A3
BOBBY
SAMPLER
Surface Water/ /G
DAN SAMPLER
Surface Water/ /G
DAN SAMPLER
Surface Water/ /G
JOHN SAMPLER
Surface Water/ /G
JOHN SAMPLER
Chain of Custody Record
Relinquished By (Date 1 Time)
1
2.
3.
4.
ANALYSIS! TAS NoJ
TURNAROUND PRESERVATIVE! Bottles
Sarr^iler
Signature:
Received By (Date 1 Time)




Case No: 39400
DAS No: OAS9000 1
SDONO: *—
For Lab Use Only
Lab Contract No:
Unit price:
Transfer To:
Lao contract No:
Unit Price:
STATION SAMPLE COLLECT ORGANIC FOR LAB USE ONLY
LOCATION BATE/TIME SAM RLE No sample condition on Receipt

1705.0(21). AES 1-4 6-2118001.5-2119007(21 LOCATION ONE 3:11/6/2008 14:57 C3TK1
(14>
AES1-4(14) 6-2119009(1) LOCATION TWO S: 11/9/2009 8:13 C3TK2
1705.0(21) 6-2119025(1) LOCATION THREE S: 11/9/2008 8:14 C3TK4
1705.0(21), AES 1-4 6-2119032. 6-2119038(2) LOCATION FIVE 8:11/9/2009 8:14 C3TK6
(14)
1705.0(21), AES 1-4 6-2119040. 8-2119046(2) LOCATION SIX 3:11/9/2009 8:14 C3TK7
(14)
Shipment for Case
corr^jleteTY
Analysis Kay:
1705.0 = TCLP Metals a
Sample(s) to be used for laboratory QC:
MC3TK1
Additional Sampler signatures}:
Coorier Tanperature
Upon Receipt:
Concentration: L = Low. M = Lew/MedliLim . H = H^gh Type/Design ate; CompasEta = C, Grab = G
Chain of Custody Seal Numbtv:
Custody Seal Intact? Shipment Iced?
mdHg by 1705.0, AES 1-4 =AES-Ba, Ca, Cr. Al
   TR Number:
                    3-043013577-050310-0003
   PR provides prellmln^By results. Requests for preliminary results vdll increase analytical costs.
   Send Copy to: Sample Management Office, Altn: Heather Bauer, CSC. 15000 Conference Center Dr.. Chantiily, VA 201 51-3819; Phone 703/818-4200; Fax
   703/818-4602
F2V5.1. 047 pafle ! Of !
                    Figure 3-3. FORMS II Lite Inorganic Traffic Report & Chain of Custody Record (Laboratory Copy)
January 2011
                        31

-------
Chapter 3 - In-field Activities
fj PZDA USEPA Contract Laboratory Program
1Gr tiif"\ Organic Traffic Report & Chain of Custody Record
Region:
Project code:
Account Code:
CERCLIS ID:
Spll ID:
Site Name/State
Project Leader:
Action:
Sampling Co:
ORGANIC
SAMPLE No.
C3TK1



C3TK2



C3TK4

C3TK5


C3TK6



C3TK7



3
QW-1 23
ACCTOOO

|f\->
IUo
REAL SITEA/A
DAN SAMPLER
Combined Rt/FS
SMITH CO,
MATRIX/ CONCI
SAWLER TYPE
Surface Water/ /G
BOBBY
SAMPLER

Surface Water/ /G
DAN SAMPLER


Surface Water/ /G
DAN SAMPLER
Surface Water/ /G
JOHN
SAMPLER
Surface Water/ /G
JOHN
SAMPLER

Surface Water/ /G
JOHN
SAMPLER










ANALYSIS/
TURNAROUND
1310.0(21), SNA
(14). CLP ARO (14),
CLP PEST (14), VOA
(14)
BNA(14), CLP ARO
(14). CLP PEST (14).
VOA (14)

13100(21)

SNA (14), CLP ARO
(14). CLP PEST (14).
VOA (14)
1310 0(21), SNA
(14). CLP ARO(14).
CLP PEST (14), VOA
(14)
13100(21), SNA
(14).CLPARO<14),
CLPPEST<14). VOA
(14)
Date shipped: 11/9/2009 Chain of Custody R

Airbill: 9876S43211234SB789 Relinquished By

Shipped to: Organic Laboratory 1
Anywhere AR 123456 2
3.

4.

Case No: 39400 D
DAS No: DAS9000
KOrd Sampler
grume
{Date /Time) Received By (Date /Time)
















TAONOJ STATION SAMPLE COLLECT INORGANIC «C
PRESERVATIVE/ Bottles LOCATION DATBT1ME SAMPLE No Type
6-2119002.6-2119003, LOCATION ONE S: 11/6/2009
6-2119004 (Ice Only).
6-2119005 (Ice Only),
6-2119006.6-2119008(6)
6-2119010.6-2119011, LOCATION TWO 8:11/9/2009
6-2119012 (Ice Only),
6-2119013 (Ice Only),
6-2119014 (5)
6-2119026(1) LOCATION THREE 8:11/9/2009

6-2119027 (Ice Only). LOCATION FOUR 8:11/9/2009
6-21 1 9028 (Ice Only) (5)

6-2119033,6-2119034, LOCATION FIVE S: 11/9/2009
6-2119035 (Ice Only).
6-2119036 (Ice Only),
6-2119037, 6-2119039(6)
6-2119041,6-2119042, LOCATION SIX 3:11/9/2009
6-2119043 (Ice Only).
6-2119044 (Ice Only).
6-2119045. 6-2119047(6)
14:57 MC3TK1 LabQC



8:13 MC3TK2 Trip Bank



8:14 MC3TK4

8:14 Rinsate


8:14 MC3TK6 PE



8:14 MC3TK7 Field Duplicate



Shipmert for Case
Complete? N
Analysis Key:

Samplejsj to be used for laboratory QC:
C3TK1
Concentration: L = Low. M = Low^edlum. H = High

Additional Sanpler Signatures):
Type/Designate: Composrte = c, Gfab = G

Chain of Custody Seal Wumfaer:
Shipment le«d?

               TR Number:
                                3-043013577-050310-0004
               PR provides prettmfnary results. Requests for prellmtnafy results will increase analytical costs.
               Send Copy to: Sample Management Office, Attn: Heather Bauer, CSC, 15000 Conference Center Dr . ChantiHy. VA 20151-3819: Phone 703/818-4200: Fax
               703/818-4602
F2VS.1.M7 page 1 of 1
                             Figure 3-4. FORMS II Lite Organic Traffic Report & Chain of Custody Record (Region Copy)
32
       January 2011

-------
                                                                                                                                  Chapter 3 - In-field Activities


•fffrFPA. USEPA Contract Laboratory Program
mF tl, r\ inorganjc Traffic Report & Chain of Custody Record


Region:
Project Code:
Account Code:
CERCLIS C
Spin ID:
Sits NamefSI Jt8
Project Leader:
Action:
Sampling Co:
INORGANIC
SAMPLE No.
MC3TK1
MC3TK2
MC3TK4
MC3TK6
MC3TK7
3
OW-123
ACCTOOO
IDS
REAL SITE/VA
DAN SAMPLER
Combined RI/FS
SMITH CO.
MATRIX! COHCI
SAWLER TYPE
Surface Water/ /G
BOBBY
SAMPLER
Surface Water/ /G
DAN SAMPLER
Surface Water/ /G
DAN SAMPLER
Surface Water/ /G
JOHN
SAMPLER
Surface Water/ IG
JOHN
SAMPLER

ANALYSIS)
TURNAROUND
1705.0<21). AES 1-4
(14)
AES 1-4(14)
1705.0(21)
1705.0(21), AES 1-4
(14)
1705,0(21), AES 1-4
(14)
Date Shipped
Carrier Name
Airbill:
Shipped to:
: 11/9/2009
FedEx
123456789987654321
Inorganic Laboratory
1 234 Watson Drive
Anywhere NC 123456
(123)456-7892

Case No: 39400 D
DAS No: DAS9000
Chain of Custody Record
Relinquished By
{Date /Time)
1
2,
3,
4.
Sampler
Signature:
Received By (Date /Time)





TAG No.,' STATION SAMPLE COLLECT ORGANIC QC
PRESERVATIVE/ Bottles LOCATION DATE/TIME SAMPLE No Tyl>e
6-2119001,6-2119007(2) LOCATION ONE 3:11/6/2009 14:57 C3TK1 LabQC
6-2119009(1) LOCATION TWO 3:11/9/2009 8:13 C3TK2 Trip Blank
6-2119025(1) LOCATION THREE 3:11/9/2009 8:14 C3TK4
6-2119032,6-2119038(2) LOCATION FIVE 3:11/9/2009 8:14 C3TK6 PE
6-2119040,6-2119046(2) LOCATION SIX 3:11/9/2009 8:14 C3TK7 Field Duplicate
Shipment for case
Complete? Y
Analysis Key;
1705.0 =TCLP Metals a
Sarrplejsj to be used for laboratory OC :
MC3TK1
Additional Sartfrter Signatures}:
Concentration; f. = LOW, M = UwAfcdlum, H = High Type/Designate: CcmpC'Slte = C. Grab = G
Chain of Custody Seal Number :
Shipment Iced?

na Hg Dy i/uti.o. A^S 1-4 = Ahi>-ba, ca, cr, AJ
          TR Number:
                           3-043013577-050310-0003
          PR provides preliminary results. Requests for preliminary results will increase analytical costs.
          Send Copy to: Sample Management Office, Aim: Heather Bauer, CSC, 15000 Conference Center Dr., Chantilly. VA 20151-3819; Phone 703/S18-4200: Fax
          703/818-4602
F2WS.1.H7 Pagal of 1
                        Figure 3-5. FORMS II Lite Inorganic Traffic Report & Chain of Custody Record (Region Copy)
January 2011
                            33

-------
Chapter 3 - In-field Activities
Page 1 cf 1

US-iPACLP Organic® COC (LAS COPY| ChWN Of CUSTOCW RBCOM> No: 39428-01 raBil (M5Q1 6
D8KtSNpp«l 2yi?2£nO<
CamerN-artte F«dEx
AiltatNa JJ41321 272121















Organic MatrrrfSBipplor Coll.
S-ampI* W MvtiKxf
B7260 Hn '-twV GilD
67261 SKvnerV GraB
B72B2 SeJffiierV GfBE)
iLiAt
67283 So . niwr'tf Grab
B7264 SfO.rnwV GraB
B7266 hu'irtiwv Grio
5EIMS
67296 " ftcintrtf " Grab
SCRAS
672B7 Sei'tYit*-tf Grab
B726S Pu'nwf Gf»h
B7309 Sip-irnf'-V' Grab
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BNAJTSl. CLP TCL PCBs(14)
CLPTCLPestiades|14i
BNA(14j. CLPTCtPCBajU).
CLP TCLPesfiades(i4i
BNA| Mi. CLP TCt PCBs^ 14),
CLP TCL Peiliade»|14i
BNAjWi, CLP TCL FCQ^14»
CLPTCLPesdcides{14i
BNA|14|. CLP TCL PCB«14).
CLP TCL Pesdades(l4|
SNA(Hi, CL' TCLPCElX'4).
CLP TCL Beifieidesi 14 1
BNAj14i, Q.nTCl rCE^U)
CLPTCLPOS!lClOC5|1+i
BNAfMi. a P TCI PCB* -4).
CLP TCL PeHHideMMl
CLP TCL Df5Hcif1csj1*i
Bf'WWl, CL^ Tgl. PCB9 ' i),
CLP TCL oesnades) I4|

.<5pWBl tnshiditn.
Analysis Key EHA=CtP TCL Ssinivolalits
ll.eiiis«f?€ason I Ra*i(|usn«l by -Date Hecaved bj Date
C^s^S 123^ Lsb. G^ti^c O^'^sflic (j3t>
Cootef * 4 L* Contact Mr lafe Contact
LatiPtioie 565-555-1234
l-octtlon
1&87. t5BS tS«9<3l ' ERT4-3-A
I69fl. isae f 597(3| ' ERT44-6
1600. T902 t6a3 (3 i ' ERT4-3-C
160$. '90S !SCS(3i ERT4-3-D
1612 1614 »61S<3| " ERT4-3-E
i$1fl. 1639 162M31 ERT4-.J-F
1624 1626 «27 (31 * ERT4-3-G
16» 1332 16330) E»T*->H
16», t«3» i8»(3| ' EHT4-3-I
164J 1544 '646(31 " ERT*-3~J
	 1645 ' SCO teOl73| ' 	 ERT4TK 	
CiHlartdd lnesg
-------
                                                                                                                          Chapter 3 - In-field Activities
    Page 1 of 1
     US EPA CLP Qr
     GamerNamc: FedEx
     Aimilfsto. S41S2127212I
CHAIN Of CWSTODY
      Scribs CLP Site
        Cooler #. 4
Mo: 3S428-01«i«f10-0016
    i^b Generic Organic l^t)
 Let) Contact. Mr Lab Contact
    Lab Pfwim 555-556-1234
Organic
B72W
B7261
B72E?
B7263
B72B4
B72I55
B7266
B72E7
B7268
B7268
B727Q
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8-ERAS
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TCLPCBs(14l §WA£14|
BNA| 141. CLP TCL PCBS 14).
CLP TCL PMtidiJW1 14)
BMAI14). CLP TCL PCBSI141.
CLP TCL P*tbddM(14)
BUM 141. CLPTCLPCBSi14).
CLP TCL PMbddK( 14)
BMA| 141. CLP TCL PCB*| 14).
CLP TCL P«B€l(J*S[ 14)
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CLP TCL P«tKld«| 14)
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CLP TCL PestWdealU)
BMfli 14| CLP TCL PCBSj 1 4).
CLP TCL P«bdd«|14)
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CLP TCL PftSbddWj 14)
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CLP TCL P«IKiessi 14)
Tagrt9 f taw waiivB/ Bottle*
1687, 15S8. 1589<3>
1594, 1596. 1597<3>
1600, 1807. 1600(3)
1606,10)6, 1600(3)
1612, 1614 1815 ;3J
161B, 162O 1621 (3)
1624, 1625 1627<3f
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164B 165D. 1651 <3f
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l:RT4-3-C
l:RT4-3-D
ERT4-3-E
ERT4-3-F
ERT4-3-G
ERT4-3-H
ERT4-3-I
ERT4--J-J
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Colioot«d
01/29^3310 11 40
01/29^2010 11 *0
01/39^2010 11 <0
01J7»^J10 1140
omaraiia 11 *o
01/2SV201Q 11 "40
D1/2»3D10 11-40
Di/29raaia 11 -40
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Inuryjnic $antplo Typo
MB? 260 neid Sample
f^872§1 Field SawpliS
MS7'?K? Held Sample
M&7263 Reid Sample
MB7264 Fisld Sarti|il*
MS7255 Flrtd Sample
M 87 256 Find Sampl»
MB7267 Field Sample
MB7268 Field Sample
M87268 Flrid Sample
MB7Z70 Field sample
     Sp«dat Inttudions,

     Analysts Key- BNA^ClP TCL SefnivoWHes
                                         Shipment for Casa Complst*? H
                                         Siirnpk'i; Tiun-jti>ir L-d FrtMll Cliuln of Custody y D&W R^Ci^^d t?V Dlftfr

,
Tim* U»ri«fR««sofi | R«linqui»«d By


P9$t RitO^V*fd by D^l^f Tirtwif

.
                                     Figure 3-7. Scribe Organics Chain of Custody Record (Region Copy)
January 2011
                                                                                       35

-------
Chapter 3 - In-field Activities
            P»g« J 
-------
                                                                                                                                 Chapter 3 - In-field Activities
      Paige 2 of 3
      USSR A CLP inorganics COC (RiGtON COPY)
      OsteStiipped: 2f 1/2010
      C-arrierNwne: FedEx
      AjrfcillNo: 8418212*218?
 CHAIN OF CUSTODY RiCORD
       Scribe CLP Site
        Casefr 123-45
         Coder #. 2
                             No: 39428-01 ,f2ai 0-0012
                                L*: Genenc inorganic Lab
                              Lab Contact Mr Lab Contact
                                Lab Phone: 565-555-1234
Inorganic
Sample #
MB7268
MB7MS
MB72?0
MB7271
MB7272
MB7273
MB7274
MB7275
MB7276
MB727?
MB727S
Matrixi'Sampler
SERAS
Sediment/
SFRAti
SERAS
Sedineti!/
SERAS
JSERAS
SERAS
SERAS
ScdHenV
SERAS
Sediment
SERAS
SediBWi!/
SERAS
SERAS
Coil.
Mel bod
Grab
Crab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
AnatygisiTurnaround \ Tag'Pieservativa.'Bottles
CUP TAL/HflCt-i; 14} | 1 635 ( 1 )
CLPTAL/HgOJ(14t i 1641(1)
CLP TAUH#CN(14J j 164? (1)
CLP TAL/HgO4{14} i 1666(1)
CLPTAUHg/CN(14) i 1659(1)
CLP TAL/HgC^14| j 1665(1)
CiP T AUHgC-M 141 | 1 87 1 ( 1 )
CLf>TAL/H8)CK(14l | 1677(1)
CtPTAUHg/CN(14) | 1683(1)
CLP TALMgCN 14| | 1 689 ( 1 )
CtJPTAUH9CN(14| i 1685(1)
Stali an
Location
ERH-3-1
ERT4-W
ERT4-3-K
ERT3-3-A
ERT3-3-B
ERT3-3-C
ERT3-3-D
ERTW-6
ERTS-:^
ERT3-3-G
ERT3-3-H
Collected
(J1/2W3310 11 40
01/2W»10 1140
01C9CT10 1140
01G9#2S10 11.00
01/29f331Q 1100
D1/2af2010 11 OQ
01^9/2010 11.00
01/29f3DtO 11.00
01/2W2010 11:00
D1/2W2Q1Q 11 00
01/2*201011:00
Organic Sample Type
Sample rt
57245.^ F«iJ Swnpl-o
R7?69 Fisl Sampifl
B i? '*o (-iftd feampfs
B?2'"1 hrtd Sample
072 r; r,e3Gamp.e
6 ??73 Fi«s.1 Sdmpla
Si274 hts-j SdinplS
B72T5 TieJ Sampis
87276 Raj Sample
67278 Re 3 Sample
      Samplers) to be used lor Lab QC  MB7276

      Analysis Key: TM-CLP IAL Total  Metas. CN=CiP CN

        items/Reason  j  Heunquisned oy  '   Date      Received Oy
                                           5(u|irrmnl for Cjnm Ct>ni|ilulu'.' N
                                           Samples Transferfed Frarn Chain ol Custody
Lwte     Time
                 Ilerns/Keaiori
«elinefs.4ish«j BY  '   Dale
                                                          Received sy
                                                                         Dale    Time
                                      Figure 3-9. Scribe Inorganics Chain of Custody Record (Region Copy)
January 2011
                                                                                             37

-------
Chapter 3 - In-field Activities
                           THIS PAGE INTENTIONALLY LEFT BLANK
38                                                                              January 2011

-------
                                                                                 Chapter 3 - In-field Activities
       3.2.3   Complete and Attach Custody Seals

                Custody seals are usually pre-printed stickers that are signed (or initialed) and dated by the sampler after
                sample collection and placed on sample bottles or containers and/or shipping coolers or containers (see
                Figure 3-10). The custody seals document the person who sealed the sample container and verify that the
                sample has not been tampered with. The seals must be placed such that they  will break if the sample
                bottle or container or the shipping cooler or container is tampered with or opened after leaving custody of
                samplers. Custody seals can also be used to maintain custody of other items such as envelopes containing
                videotapes of the sample collection process. Instructions for completing and attaching a custody seal are
                included in Table 3-5.

                The use and type of custody  seals can vary  by Region or collecting organization. Samplers should obtain
                the appropriate custody seals and specific instructions for correctly attaching them from the RSCC.

                        Custody seals should never be placed directly onto a coring tool used as a transport device
                        (e.g., 5  g Sampler) or tared, 40 mL closed-system vials. The seals must be placed on the bag
                        for the coring tool used as a transport device, or on the bag used to enclose the vials. Refer to
                        Appendix B for details.

^lost,, UNITED STATES
.» _. ** ENVIRONMENTAL PROTECTION AGENCY
JB A \ OFFICIAL SAMPLE SEAL
yssf
\*x

SAMPLE NO-
DATE
SIGNATURE
PRINT NAME AND TITLE
SEAL BROKEN BY
uj
s


                                         Figure 3-10. Custody Seal

                           Table 3-5. Completing and Attaching a Custody Seal
Step
1
2
3
4
5
6
Action
Record the CLP Sample number.
Record the month, day, and year of sample collection.
Sign the seal in the signature field.
Print your name and title in the "Print Name and Title"
field.
Place the custody seal over the edge of the sample bottle or
container such that it will break if tampered with.
If possible, cover the custody seal with clear plastic tape to
protect it.
Important Notes
The space for the CLP Sample number does not need to be
completed on custody seals being placed on the opening of a
cooler, only on those being placed on the opening of sample
bottles or containers.



Custody seals can be placed directly on any sample container
except for coring tools used as a transport device (e.g., 5 g
Samplers) and tared VOA bottles. If packing coring tools
used as a transport device or tared VOA bottles, place them in
a clear plastic bag and place the custody seal on the outside of
the bag.
Take special care to not place the protective tape over the seal
in such a way that it can be removed and then re-attached
without signs of tampering.
       3.2.4   Complete and Attach Sample Labels

                Samplers affix sample labels to each sample container. A sample label must contain the associated CLP
                Sample Number (either written or pre-printed), SMO-assigned CLP Case Number,  and the preservative
                used. It  must also denote the  analysis. Samplers may also include additional information such as  the
                station location or the date/time of collection. Samplers should use FORMS II Lite or Scribe to create and
                print sample labels. The sampler can print two labels and attach one to the sample container or bottle, and
                place the other label on the sample tag that should also be attached to the sample container or bottle. The
                labels should then be covered with clear packaging tape  to protect the label and maintain legibility. If
January 2011
39

-------
Chapter 3 - In-field Activities
               handwriting a sample label, the sampler should complete the label information using waterproof ink,
               place the label on the outside of the sample bottle or container, then cover the label with clear packaging
               tape to protect the label and maintain legibility (see Figure 3-1).
                       Do not attach labels to tared VOA sample vials. Avoid wrinkles in the tape and labels.
       3.2.5  Complete and Attach Sample Tags

               To support use of sample data in potential enforcement actions, sample characteristics other than on-site
               measurements (e.g., pH, temperature, conductivity) can be identified with a sample tag. Typically, site-
               specific information is written on the tags using waterproof ink. The use and type of sample tags may
               vary by Region. For each sampling event,  samplers should contact the RSCC concerning the use  of
               sample tags and the type of information required for the tags. An example of a completed sample  tag is
               included in Figure 3-11 below:


Project Code 2
OO-030
£ £
8 ^
O r-
O E
O •
2 w
tu
z
o
Station No. 3
Mo./Day/Year 4
01 /J (0/2(0(04
Station Location T
3J
3
m
u>
1



w
o
01
3
o'
in

Pesticides

VOA organics
X
DO




Time 5
08:45

Designate: 6
Comp.
Sampler's (Signature)
/(^in/Smttfv


o
0)
3



5.
2.
ST


Phenolics

COD, TOO, Nutrients

O~t>
3!
cow
"coo.
— u>

Grab
X
8
ANALYSES ®
Preservative:
Ym NCH «
©


                                   Figure 3-11. Completed Sample Tag

               The sampler can use FORMS II Lite or Scribe  to create and print out multiple sample labels, one of
               which can be attached to the sample tag and then covered with clear packaging tape to protect the label
               and maintain legibility.  If FORMS II Lite- or Scribe-created sample labels are not available, a detailed
               set of instructions for completing and attaching a handwritten sample tag are included in Table 3-6.

                          Table 3-6. Completing and Attaching a Handwritten Sample Tag
Step
1
1
3
4
5
6
1
8
9
10
Action
Under the "Remarks" heading, record the CLP Sample Number and SMO-assigned CLP Case Number in a
legible manner.
Record the project code (e.g., Contract number, Work Assignment number, Interagency Agreement
number, etc.) assigned by USEPA.
Enter the station number assigned by the sampling team coordinator.
Record the month, day, and year of sample collection.
Enter the military time of sample collection (e.g., 13:01 for 1:01 PM).
Identify the designate and place an "X" in either the composite (Comp.) or grab (Grab) sample box.
Record the station location.
Sign the sample tag in the signature area.
Place an "X" in the box next to Yes or No to indicate if a preservative was added to the sample.
Under "Analyses", place an "X" in the box next to the parameters for which the sample is to be analyzed.
40
January 2011

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                                                                                    Chapter 3 - In-field Activities

Table 3-6. Completing and Attaching a Handwritten Sample Tag
Step
11
12
Action
Leave the box for "Laboratory Sample Number" blank.
It is recommended that the sample tag be attached to the neck of the sample bottle or container using regular
string, stretch string, or wire.
3.3    Provide Sample Receipt

        After samples have been taken from private property, the sampler should prepare a receipt for these samples and
        provide this receipt to the property owner. This is especially important when sampling on private property since
        these samples could be used during future litigation and the receipt will verify that the owner granted approval for
        the removal of the samples from the property. An example of a sample receipt created using FORMS II Lite is
        shown in Figure 3-12. An example of a sample receipt created using Scribe is shown in Figure 3-13.
                     Regions
             U.S. ENVIRONMENTAL PROTECTION AGENCY
                                                  RECEIPT FOR SAMPLES
            PROJECT NO,
            SAMPLERS; (SIGNATURES)
                                                 EXAMPLE SI H-,
            STATION NO.
                      LOCATION /DESCRIPTION
                                                                   SPLIT
                                                           NO. OP iPA  SAMPLE
                                         DATE    TIME  Camp/Grab CONTAINERS   V Oft N EPA SAMPLE TA6 MO.'S
            STATION ONE
            STATION ONF
            SIATION TWO
LOCATION CfiE
i COTTON TWO
LOCATION CflE
2/20/2X1   15:55  Ci
?1p'?0/7'3Cl   Ifi'U  C
2/2G/20C1   lfc:C2  f
SPLIT SAMPLES TRANSFERRED BY:
BMHtfTS
""
DATE
TIME
SPLIT SAMPLES HiCIIVID 8Y C OR DECLINED BY D
sawn
DATE/TIME
TfLfPHOKf
                                                                                         F»S.I.«I fjy, i „( |
                  Figure 3-12. Sample Receipt Created Using the FORMS II Lite Software
January 2011
                                                                                       41

-------
Chapter 3 - In-field Activities
  Pays 1 of J
   Project No: C45R020
   Sampies Transferred:
   Samples Received By:
   Receipt for Samples
Samples Residential Sampling


  Project Name: Demc Project
  Signature:
  Signature:
                                                                                               WA Number 0003
Samples Signature:
S-ample #
Sample Date
EventlD
Locafeon
Property! D
Matrix
Collection Method
Sample Type
Analyses
Tag
Container
COC
Lab
Remarks

SS-0002
6W2QIO
Front Yard Soi* Sampling
H002-F
H002
Sot-
Grab
Fieid Sampie
TAL-Metals-801QB
A
Zipioc
SsiOOl
ACME Environmental LaD


SS-0002
6/8/2010
Front Yard Soil Samp-iing
H002-F
HOD2
Sos
Grab
Field Sampie
CLP TCL Vdatiles
1003
40 m' Vial
CLP Ohain-0001
ABC Special Lab


SS-0002
8)9/2010
Front Yard Soii Sampling
H002-F
H002
Soil
Grab
Field Sample
CLP TCL Semwotatrles
1002
4az Glass
CLPChain-0001
ABC Special Lab


SS-OOD8
6,1912010
PCS Sampling
H002-F
H002
Sol
Grab
Field Sample
PCBs
A
1 8 oz glass
So.i-002
ABC Special Lab


Sample #
Sample Date
EtfefitID
Location
Property! D
Matrix
Collection Method
Sample Type
Analyses
Tag
Container
COC
Lab
Remarks

ss-0009
6/9/2010
PCS Sampling
H002-R
H002
So*
Grab
Fiefc Sample
PCBs
A
1 6 oz glass
Scifi-002
ABC Special Lab


SS-0017
6/9/2010
E-acfc Yard Soil Samp^ng
H002-R
H002
Soi
Grab
Field Sample
TAL-Metals-eo10B
A
ZipSoc
St}t!-GQ3
ACME Environmental Laa


SS-0017
M,'2010
Back Yard Soi) SampSng
HOD2-R
H002
Saii
Grab
Field Sample
CLP TCL Vclaales
1013
40 ml Vial
S-O8251 0-1 35425-0004
ABC Special Lab


SS-0017
8.OQ010
Bac^ Yard Soi! Sa.^pS*ng
HCM2-R
H002
Sod
Grab
Field Sample
CiP TCL Semiraatiles
1012
4oz Glass
6-062510-135425-0004
ABC Special Lab


                      Figure 3-13. Sample Receipt Created Using the Scribe Software

3.4    Pack and Ship Samples

        Once the samples have been collected,  it is very important that the sampler properly  package the samples for
        shipment and ensure that the samples are sent to the appropriate laboratory as quickly as possible. Prompt and
        proper packaging of samples will:

        •   Protect the integrity  of samples from changes in composition or concentration caused by bacterial growth or
            degradation from increased temperatures;
        •   Reduce the chance of leaking or breaking of sample containers that would result in loss of sample volume,
            loss of sample integrity, and exposure of personnel to toxic substances; and
        •   Help ensure compliance with shipping regulations.

       3.4.1    Sample Containers

                Once samples are collected, they must be stored in conditions that maintain sample integrity. All samples
                should be placed in shipping containers or other suitable containers with ice to reduce the temperature as
                soon as possible after collection.

                Ideally, all samples should be shipped the day of collection for overnight delivery to the laboratory. If
                samples cannot be shipped on the day of collection, the sample temperature should be maintained at 4°C
                (±2°C) until they are shipped to the laboratory.
42
                                                   January 2011

-------
                                                                               Chapter 3 - In-field Activities
               One CLP RAS sample may be contained in several bottles and vials. For example, one water sample may
               consist of all containers needed for three of the analytical analyses available under this service (i.e.,
               SVGA analysis, Pesticide  analysis, and Aroclor analysis),  even though the analyses are collected in
               separate containers. Therefore, the analysis to be performed  and the matrix type will determine the type
               of container(s) that will be  used, as well as the volume that  must be collected for that particular sample
               analysis.

       3.4.2  Inventory of Samples and Documentation

               Prior to shipment, samplers  should conduct an inventory  of the contents of the shipping cooler or
               container against the corresponding TR/COC Record when packing for shipment to laboratories. An
               inventory will ensure that the proper number of containers have  been collected for each analysis of the
               samples, that the required PE and QC samples and cooler temperature blanks are included, and the correct
               Sample Numbers and analyses have been assigned to each sample. Refer to Appendix G and Appendix H
               when referencing the CLP analysis codes.

       3.4.3  Shipping Regulations

               Sample shipping personnel are legally  responsible for ensuring  that the sample shipment will comply
               with all applicable shipping regulations. If dry ice is used for shipping tissue samples, special packaging
               precautions are required before shipping by aircraft to comply with U.S. DOT regulations. Refer to the
               Code of Federal Regulations (49CFR 173.217) classified dry ice as Hazard Class 9 UN 1845 (Hazardous
               Material).  For  example, hazardous  material samples  must  be packaged, labeled, and shipped in
               compliance with all IATA Dangerous  Goods regulations or DOT regulations and USEPA guidelines.
               Refer to Appendix B for detailed shipping guidelines when using SW-846 Method 5035A to preserve and
               ship samples.

       3.4.4  Sample Packaging for Shipment

               Samplers are responsible for the proper packaging  of samples  for shipment. To ensure that samples are
               appropriately packaged  (e.g.,  to avoid breakage and/or contamination) the sampler should consult their
               respective project plans to determine the proper packing and shipping procedures. The sampler  must
               determine the sample type, pack the shipping containers correctly, include necessary paperwork,  label
               and seal shipping containers or coolers, and ship the samples.

               3.4.4.1   Determine the Sample Type and Container

                         Samplers should know what kinds of samples they  are handling to ensure proper packaging.
                         Samplers should refer to their appropriate project plans to  determine which type of sample
                         container should be used for each type of sample being taken during the sampling event.

                                  Please follow Regional guidance with  reference to samples containing dioxin or
                                  radioactive waste.

               3.4.4.2  Pack Shipping Containers

                         It is imperative  that samples are  correctly and  carefully packed in shipping containers to
                         prevent the sample containers from breaking or leaking.  Samplers must prepare and pack a
                         shipping cooler or container according to the instructions outlined in Table 3-7.
January 2011                                                                                           43

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Chapter 3 - In-field Activities
                              Table 3-7. Packing Samples for Shipment
Step
1
2
3
4
5
6
7
8
9
10
11
Action
Seal all drain holes in the shipping container, both inside and out, to
prevent leakage in the event of sample breakage.
Check all lids/caps to make sure the samples are tightly sealed and
will not leak.
Wipe loose soil residue from containers.
Seal samples within a clear plastic bag.
Fully chill samples to 4°C (±2°C) prior to placement within suitable
packing materials.
Prior to placing samples within the shipping cooler, it is
recommended that samplers line shipping containers with non-
combustible, absorbent packing material.
Place samples in CLEAN, sealed, watertight shipping containers
(metal or hard plastic coolers).
Conduct an inventory of the contents of the shipping cooler/container
against the corresponding TR/COC Record.
Cover samples in double-bagged ice to prevent water damage to
packing materials.
It is recommended that a temperature blank be included in an area
within each shipping container which will allow for easy access by
the laboratory upon opening the shipping container.
Ensure that the site name or other site-identifying information does
not appear on any documentation being sent to the laboratory.
Important Notes



Custody seals can be placed directly on any sample
container except for coring tools used as a transport
device (e.g., 5 g Samplers) and tared VOA bottles.
If packing coring tools used as a transport device or
tared VOA bottles, place them in a clear plastic bag
and place the custody seal on the outside of the bag.

The CLP strongly discourages the use of
vermiculite and cat litter as sources for packing
material. These materials interfere with labeling
and documentation and are difficult to remove from
sample containers and shipping containers.
All soil/sediment samples known to contain dioxin
should be securely enclosed in metal cans (e.g.,
paint cans) with the lids sealed.

Do NOT pour loose ice directly into the sample
cooler. The ice is used to maintain the temperature
of the samples within the shipping cooler.
The temperature blank is generally a 40 mL vial
filled with water and labeled "temperature blank"
but does not have a Sample Number.

              3.4.4.3  Include Necessary Paperwork

                       Samplers must properly place the necessary paperwork in the shipping cooler or acceptable
                       container. All paperwork must be placed in a plastic bag or pouch and then secured to the
                       underside of the shipping container lid (see Figure 3-14).
                  SAMPLE DOCUMENTATION
    Figure 3-14. Sample Cooler with Attached TR/COC Record, PES Instructions (if applicable), and
                                   Cooler Return Documentation
44
January 2011

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                                                                              Chapter 3 - In-field Activities
                         Necessary paperwork includes TR/COC Records and sample weight logs (see Figures 3-15
                         and 3-16), if required (for VOA samples). If PE samples are included in the cooler, the PE
                         instruction sheets must be included with the paperwork. Samplers should contact their RSCC
                         (or designee) for specific paperwork requirements.
USEPA Contract Laboratory Program
Sample Weight Log

shipped to: AAA Testing Laboratory
1700 Mill Avenue
Houston TX 77099
(281)983-1234
Case No. 39563
DAS No. DAS34
Date Shipped: 9/29/2003

Sample No. Matrix Analysis Preservative Bottle/ Tared Weight Final Weight Sample Weigh! Laboratory Traffic Report
Tag Number (g) (g) (g) Weight No.

C0036 Subsurface CLP TCL Volatiles Ice Only 199548
Soil (>1 2")
C0036 Subsurface CLP TCL Volatiles Ice Only 199547
Soil (>1 2")
C0036 Subsurface CLP TCL Volatiles Ice Only 199549
Soil (>f 2")
C0037 Surface Soil CLP TCL Volatiles Ice Only 199552
C0037 Surface Soil CLP TCL Volatiles Ice Only 199551
C0037 Surface Soil CLP TCL Volatiles Ice Only 199550
Completed By:
All weights are measured in grams
3280 37.20 4.40 3-103018225-092903-0001
32.10 38.30 6.20 3-103018225-092903-0001
31.20 38.60 7.40 3-103018225-092903-0001
3200 36.90 4.90 3-103018225-092903-0001
32.40 37.10 4.70 3-103018225-092903-0001
31.90 35.90 4.00 3-103018225-092903-0001
Date:


                            Figure 3-15. FORMS II Lite Sample Weight Log
Page 1 of 1
Sarrple Weight Log
Chain of Custody - Additional Info - VQA. Samples
Shipped To: ABC Special Lab Completed By. Case Ho. 12346
123 Main Street DAS Ho. DAS123
Anyiu here , US A Date: Date Shipped : 1 2/24CQ 1 0
CLP Sample #
Matrix
Analyses
Preservative
Tag
Tared Weight (g)
Rnal Weight (g)
Sample Weight (g)
Laboratory Weight
coca

YQ004
Soil
CLP TCL Volatile*
be
1011
32 SO
37 20
4.4

9-062510-135425-
0004
•moos
Soil
CLP TCL Volatile*
Ice
1013
32.10
38.30
6.2

9-062510-135425-
0004
Y0006
Soil
CLPTCLVblatiles
Ice
1015
3120
38 BO
7.4

9-0625 10- 135425-
0004
YDQD7
Soil
CLP TCL Vblatiles
Ice
1017
32.00
36.90
4.9

9-062510-135425-
0004
Y0008
Soil
CLPTCLVblatiles
Ice
1019
3130
3530
4

9-062510-135425-
0004
                                 Figure 3-16. Scribe Sample Weight Log

               3.4.4.4  Label and Seal Sample Shipping Coolers

                         After samples are packaged within shipping coolers, samplers must carefully secure the top
                         and bottom of the coolers with tape,  place return address labels clearly on the outside of the
                         cooler, and attach the required chain-of-custody seals (see Figure 3-17).
January 2011
45

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Chapter 3 - In-field Activities
            CUSTODY SEALS
46
                      Figure 3-17. Shipping Cooler with Custody Seals

                  If more than one cooler is being delivered to a laboratory, samplers should mark each cooler
                  as "1 of 2", "2 of 2", etc. In addition, samplers must accurately complete and attach shipping
                  airbill paperwork for shipment of the samples to the laboratory. An airbill, addressed to the
                  Sample Custodian of the  receiving laboratory, should be completed for each cooler shipped.
                  Samplers should receive the correct name, address, and telephone number of the laboratory to
                  which they must ship samples from the RSCC or SMO. To avoid delays in analytical testing,
                  samplers  should make sure they are sending the  correct types of samples to the correct
                  laboratory when collecting  samples  for multiple types  of analysis.  For example, inorganic
                  samples may be shipped to one laboratory for analysis, while organic samples may need to be
                  shipped to another laboratory.

        3.4.4.5  Ship Samples

                  The sampling contractor  should  ensure that samplers know the shipping company's name,
                  address, and telephone  number. In addition, they should be aware of the shipping company's
                  hours of operation, shipping schedule, and pick-up/drop-off requirements.

                 Overnight Delivery

                  It is imperative that samples be sent via overnight delivery. Delays caused by longer shipment
                  times may cause technical holding times to expire, which in turn may destroy sample integrity
                  or require the recollection of samples for analysis.

                 Saturday Delivery

                  For shipping samples for Saturday delivery, the sampler MUST notify the RSCC (or their
                  designee) and SMO as  soon as possible so that SMO will receive the delivery information by
                  3:00 PM ET on the Friday prior to delivery.

3.4.5   Shipment Notification

        When samples are shipped to CLP Laboratories, samplers must immediately report all sample shipments
        to the  RSCC (or  designee) or to SMO. Under no circumstances should the  sampler contact  the
        laboratory directly. If samplers are  shipping samples after 5:00 PM ET, they must notify  the RSCC (or
        designee) or SMO by 8:00 AM ET on the following business day. Samplers should receive the name and
        phone number of the appropriate SMO coordinator to contact from the Region/RSCC.
        Samplers must provide the following information to the RSCC (or designee) or to SMO:

        •   Name and phone  number at  which they can easily be reached (preferably  closest  on-site phone
            number if still in the field)
        •   SMO-assigned CLP Case Number (see Section 2.4.1)
        •   Number, concentration, matrix and analysis of samples being shipped, and MA number (if required);
        •   Name of laboratory (or laboratories) to which the samples were shipped
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                                                                                Chapter 3 - In-field Activities
                •   Airbill number(s)
                •   Date of shipment
                •   Case status (i.e., whether or not the Case is complete)
                •   Problems encountered, special comments, or any unanticipated issues
                •   When to expect the next anticipated shipment
                •   An electronic export of the TR/COC Record must be sent as soon as possible after sample shipment
                   via the Electronic Sample Documentation System  (ESDS) to  transmit FORMS II Lite and Scribe
                   files to SMO. For information regarding electronic export of TR/COC Records, refer to the following
                   Web site:

                          http://www.epa.qov/superfund/proqrams/clp/download/esdsprocedures.pdf

                       For Saturday  delivery, samplers MUST notify the RSCC (or designee) and SMO as soon as
                       possible so that  SMO will receive the delivery  information by 3:00 PM ET on the Friday prior
                       to delivery.

                Samplers should be aware if their Region requires them to notify the RSCC (or designee) and/or SMO of
                sample shipment.

       3.4.6   Return Sample Shipping Coolers

                CLP laboratories must  routinely return  sample shipping coolers within 14 calendar  days following
                shipment receipt. Therefore,  the sampler  should also include cooler return  instructions with each
                shipment. The sampler  (not the CLP laboratory) is responsible for paying for return of the cooler and
                should also include shipping airbills bearing the sampler's account number, as well as a return address to
                allow for cooler return. Samplers should use the least expensive option possible.
January 2011                                                                                           47

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                                                                              Appendix A
              Appendix A: Functions within a Sampling Project
The following table describes Quality Assurance Project Plan (QAPP) requirements taken from EPA Requirements for
Quality Assurance Project Plans (EPA QA/R-5).
Functions Within a
Sampling Project
Elements of that Function
Project Management
Project/Task Organization
Problem Definition/Background
Project/Task Description
Quality Objectives and Criteria
Special Training/Certification
Documents and Records
Identifies the individuals or organizations participating in the project and defines their specific
roles and responsibilities.
States the specific problem to be solved or decision to be made and includes sufficient
background information to provide a historical and scientific perspective for each particular
project.
Describes the work to be performed and the schedule for implementation to include:
X Measurements to be made during the course of the project
X Applicable technical, regulatory, or program-specific quality standards, criteria, or objectives
X Any special personnel and equipment requirements; assessment tools needed
X A work schedule and any required project and quality records, including types of reports
needed
Describes the project quality objectives and measurement performance criteria.
Ensures that any specialized training for modified field sampling techniques, field analyses,
laboratory analyses, or data validation should be specified.
X Itemizes the information and records that must be included in the data report package and
specifies the desired reporting format for hard copy and electronic forms, when used.
X Identifies any other records and/or documents applicable to the project such as audit reports,
interim progress reports, and final reports that will be produced.
X Specifies or references all applicable requirements for the final disposition of records and
documents, including location and length of retention period.
Data Generation and Acquisition
Sampling Process Design
(Experimental Design)
Sampling Methods
Sample Handling and Custody
X Describes the experimental design or data collection design for the project.
X Classifies all measurements as critical or non-critical.
X Describes the procedures for collecting samples and identifies sampling methods and
equipment. Includes any implementation requirements, support facilities, sample
preservation requirements, and materials needed.
X Describes the process for preparing and decontaminating sampling equipment to include the
disposal of decontamination by-products, selection and preparation of sample containers,
sample volumes, preservation methods, and maximum holding times for sampling,
preparation, and/or analysis.
X Describes specific performance requirements for the method.
X Addresses what to do when a failure in sampling occurs, who is responsible for corrective
action, and how the effectiveness of the corrective action shall be determined and
documented
X Describes the requirements and provisions for sample handling and custody in the field,
laboratory, and transport, taking into account the nature of the samples, the maximum
allowable sample holding times before extraction and analysis, and the available shipping
options and schedules.
X Includes examples of sample labels, custody forms, and sample custody logs.
January 2011
A-1

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Appendix A
     Functions Within a
       Sampling Project
                                                          Elements of that Function
 Analytical Methods
                                     Identifies  the  analytical methods and equipment  required, including sub-sampling  or
                                     extraction methods, waste disposal requirements (if any), and specific method performance
                                     requirements.
                                     Identifies analytical methods by number, date, and regulatory citation (as appropriate). If a
                                     method allows the user to  select from various options, the  method citations should state
                                     exactly which options are being selected.
                                     Addresses what to do when a failure in the analytical system  occurs, who is responsible for
                                     corrective action, and how the effectiveness of the corrective action shall be determined and
                                     documented.
                                     Specifies the laboratory turnaround time needed, if important to the project schedule.
                                     Specifies whether a field sampling and/or laboratory analysis Case Narrative is required to
                                     provide a complete description of any difficulties encountered  during sampling or analysis.
 Quality Control (QC)
                                     Identifies required measurement QC checks for both the field and laboratory.
                                     States the frequency of analysis for each type of QC check, and the spike compounds sources
                                     and levels.
                                  X  States or references the required control limits for each QC check and corrective action
                                     required when control limits are exceeded and how the effectiveness of the corrective action
                                     shall be determined and documented.
                                  X  Describes or references the procedures to be used to calculate each of the QC statistics.
Instrument/Equipment Testing,
Inspection, and Maintenance
                                      Describes  how inspections  and  acceptance  testing  of environmental  sampling  and
                                      measurement systems and their components will be performed and documented. Identifies
                                      and discusses the procedure by which final acceptance  will be performed by independent
                                      personnel.
                                      Describes how deficiencies are to be resolved and when re-inspection will be performed.
                                      Describes  or  references how  periodic  preventative  and  corrective  maintenance  of
                                      measurement or test equipment shall be performed.
                                      Identifies the equipment and/or system requiring periodic maintenance.
                                      Discusses how the  availability  of spare  parts identified in the operating guidance and/or
                                      design specifications of the systems will be assured and maintained.
 Instrument/Equipment
 Calibration and Frequency
                                     Identifies all tools, gauges, instruments, and other sampling, measuring, and test equipment
                                     used for data collection activities affecting quality that must be controlled, and at specific
                                     times, calibrated to maintain performance within specified limits.
                                     Identifies the certified equipment and/or standards used for calibration.
                                     Describes or references how calibration will be conducted using certified equipment and/or
                                     standards with known valid relationships to nationally recognized performance standards. If
                                     no such standards exist, documents the basis for calibration.
                                     Indicates how records of calibration shall be maintained and traced to the instrument.
 Inspection/Acceptance of
 Supplies and Consumables
                                     Describes how and by whom supplies and consumables shall be inspected and accepted for
                                     use in the project.
                                     States acceptance criteria for such supplies and consumables.
 Non-direct Measurements
                                     Identifies any types of data needed for project implementation or decision-making that are
                                     obtained from non-measurement sources (e.g., computer databases, programs, literature files,
                                     historical databases).
                                     Describes the intended use of data.
                                     Defines the acceptance criteria for the use of such data in the project.
                                     Specifies any limitations on the use of the data.
 Data Management
                                     Describes the project data management scheme, tracing the data path from generation in the
                                     field or laboratory to their final use or storage.
                                     Describes or references the standard record-keeping procedures, document control system,
                                     and the approach used for data storage and retrieval on electronic media.
A-2
                                                                                                          January 2011

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	Appendix B

   Appendix B: CLP Sample Collection  Guidelines for VOAs in Soil by

                                    SW-846  Method 5035A


A.    Preferred Options for the Contract Laboratory Program (CLP) are Options 1, 2, and 3:

     This method employs sample vials that are filled and weighed in the field and never opened during the analytical
     process. As a result, sampling personnel should be equipped with a portable balance capable of weighing to 0.0 Ig.


                Soil  samples must be placed on their sides  prior to being frozen or placed on ice.  Dry ice or field
                freezers are the only options.


        Option  1.

            Closed-system Vials:
            Container -  tared or preweighed 40 mL VOA vial  containing a magnetic stir bar.

            Collect 5 g of soil per vial (iced or frozen in the field). Check the pre-tared weight of the (dry)  VOA vials
            prior to departure for the sampling  event under controlled conditions. Weigh vials  and soil samples to the
            nearest 0.01 g. This check is to ensure that the original weight was properly recorded.

            Regular Samples          3 Vials - Dry (5 g soil per vial)
                                     1 Vial - Dry (filled with soil, no headspace)
                                     4 Total Vials

           Regular Samples           9 Vials - Dry (5 g soil per vial)
           Requiring QC Analysis      1 Vial - Dry (filled with soil, no headspace)
                                     10 Total Vials

        Option  2.

            Closed-system Vials Containing Water:
            Container -  tared or pre-weighed 40 mL VOA vial containing a magnetic stir bar and 5 mL
            water.

            Collect 5 g of soil per vial (iced or frozen in the field). Weigh vials and soil samples to the nearest 0.01 g.

            Regular Samples          2 Vials with water added (5 g soil and 5 mL water per vial)
                                     1 Vial - Dry (5 g soil in vial)
                                     1 Vial - Dry (filled with soil, no headspace)	
                                     4 Total Vials (2 with water and 2 dry)

           Regular Samples           6 Vials with water added (5 g soil and 5 mL water per vial)
           Requiring QC Analysis      1 Vials - Dry (5 g soil per vial)
                                     1 Vial - Dry (filled with soil, no headspace)	
                                     8 Total Vials (6 with water and 2 dry)

        Option 3.

            Container - 5 g Samplers or equivalent and coring tool used as a transport device.


                   All Samplers should be iced or frozen in the field and bagged individually.


            Regular Samples           3 Samplers (5 g soil per Sampler)
                                     1 Vial - Dry (filled with soil, no headspace)
                                     4 Total (3 Samplers and 1 Vial)


January 2011                                                                                       B-1

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Appendix B	


           Regular Samples            9 Samplers (5 g soil per Sampler)
           Requiring QC Analysis      1 Vial - Dry (filled with soil, no headspace)
                                      10 Total (9 Samplers and 1 Vial)

B.   Options 4, 5, and 6 are NOT preferred options for the CLP:

        Option 4.

            Closed-system Vials:
            Container -  tared  or  preweighed 40 mL VOA  vial  containing  a magnetic  stir  bar  and
            preservative.
            Collect 5 g of soil per vial and add Sodium bisulfate (NaHSO4) preservative (5 mL water + 1 g NaHSO4) -
            iced in the field.

            Caution:      This option is NOT a  Preferred Option for the CLP because:

                          NaHSO4 preservation creates low pH conditions that will cause the destruction of certain CLP
                          target analytes (e.g., vinyl chloride,  trichloroethene, trichlorofluoromethane, cis-  and trans-
                          1,3-dichloropropene). Projects requiring the quantitation of these analytes should consider
                          alternative sample preservation methods. NaHSO4 also cannot be used on carbonaceous soils.
                          Check the soil before using this method of collection!  Soil can be checked by placing a test
                          sample in a clean vial, then adding several drops of NaHSO4 solution. If the soil bubbles, use
                          Option 4b and note this issue on the TR/COC Record.

        Option 4a.    Samples preserved in the field

            Regular Samples           2 Vials with NaHSO4 preservative added (5g soil per vial)
                                      1 Vial without NaHSO4 preservative added (5g soil per vial)
                                      1 Vial - Dry (filled with soil, no headspace)	
                                      4 Total Vials (2 with NaHSO4 preservative and 2 without)

           Regular Samples            6 Vials with NaHSO4 preservative added (5g soil per vial)
           Requiring QC Analyses     3 Vials without NaHSO4 preservative added (5 g soil per vial)
                                      1 Vial - Dry (filled with soil, no headspace)	
                                      10 Total Vials (6 with NaHSO4 and 4 without)

        Option 4b.    Samples are preserved by the laboratory (No NaHSO4 preservative is added to these samples in the
                      field).

            Regular Samples           3 Vials - Dry (5 g soil per vial)
                                      1 Vial - Dry (filled with soil, no headspace)
                                      4 Total Vials

           Regular Samples            9 Vials - Dry (5 g soil per vial)
           Requiring QC Analyses     1 Vial - Dry (filled with soil, no headspace)
                                      10 Total Vials

        Option 5.

            Methanol Preservation (medium-level analysis only):
            Container - tared or pre-weighed 40 mL VOA vials containing 5 mL methanol.

            Collect 5 g of soil per vial (iced in the field).

            Caution:     This is NOT a preferred option for the CLP because:

            Samples preserved with methanol can only be analyzed by the  medium-level method. Low-level Contract
            Required Quantitation Limit (CRQLs) cannot be achieved when samples are preserved this way. If this soil
B-2                                                                                          January 2011

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          	Appendix B

            option is used, then samples for low-level analysis by one of the other options should also be collected and
            accompany the medium-level soil.

            Additional problems associated with use of methanol as a preservative in the field include:

            •   Possible contamination of the methanol by sampling-related activities (e.g., absorption of diesel fumes
                from sampling equipment);
            •   Leakage of methanol from the sample vials during shipping, resulting in loss of VOAs prior to analysis.

            Regular Samples           2 Vials (5 g soil and 5 mL methanol per vial)
                                       1 Vial - Dry (filled with soil, no headspace)	
                                       3 Total Vials (2 with methanol and 1 dry)

           Regular Samples            6 Vials (5 g soil and 5 mL methanol per vial)
           Requiring QC Analyses      1 Vial -Dry (filled with soil, no headspace)	
                                       7 Total Vials (6 with methanol and 1 dry)


                    If  shipping samples  containing methanol as a preservative, a shipping  label must be used to
                    indicate methanol. This label must also contain the United Nations (UN) identification number for
                    methanol (UN 1230), and indicate Limited Quantity.

        Option 6.

            Glass Containers filled with sample - No Headspace:
            Container - 4 oz  Glass Jars.

            Glass container filled with soil with no headspace and iced.

            Caution:     This is NOT a preferred option for the CLP because:
                          Samples collected in this manner lose most of their volatile analytes prior to analysis when the
                          sample containers are opened and sub-sampled in the laboratory. This option is only available
                          due to Regional requirements.
            Regular Samples           2 Glass Jars (4 oz) filled with sample, no headspace
                                       1 Vial - Dry (filled with soil, no headspace)	
                                       3 Total Containers

           Regular Samples            2 Glass Jars (4 oz) filled with sample, no headspace
           Requiring QC Analyses      1 Vial - Dry (filled with soil, no headspace)	
                                       3 Total Containers

C.    Caution:

      1.  Extreme care must be taken to ensure that frozen or iced samples do not break during shipment.

      2.  Before adding soil to pre-weighed vials containing a stir bar, weigh the vials to confirm the tared weight. If the
         weight varies by more than 0.1 g, record the new weight on the label and the sample documentation. Do NOT
         add labels to these vials once the tared weight has been determined or confirmed.

D.    Dry Samples:

      All options include taking a sample in a dry 40 mL VOA vial (or a 4 oz  wide mouth jar) with no headspace. No
      water, NaHSO4,  or methanol is added to this sample. This sample is taken to determine moisture content; therefore, it
      does not need to  be tared or have a stir bar.

E.    Iced or Frozen Samples:

      1.  Iced means cooled to 4°C (±2°C) immediately after collection.

      2.  Frozen means cooled to between -7°C and -15°C immediately after collection.

      3.  Dry ice is not a long-term freezing agent and may contaminate samples.

January 2011                                                                                            B-3

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Appendix B	

F.    Sample Delivery:

      CLP strongly recommends that all samples reach the laboratory by COB the next day after sample collection.

G.    Notes:

      1.  For Options 2, 4, and 5, check the weight of the pre-tared VOA vials plus liquid in the field due to the possibility
         that liquid leaked out during packing, transit, or deployment in the field just prior to sampling. This check is to
         ensure that the original weight is properly recorded.
      2.  For Option 4, samples can be preserved with NaHSO4 either:
          •   In the field; or
          •   In the laboratory upon  receipt. In this case,  the sampler should put the  following information in the
              Preservation Column of the TR/COC Record - "To  be  preserved at lab with NaHSO4".  This Regional
              request should also be communicated to SMO so that the laboratory can be notified.

      3.  Regional  QAPPs may require the use of Option 5. Please note that this  option is  for medium-level analysis
         ONLY.

      4.  If water,  methanol, or NaHSO4 preservative is added to  the vials in the field, a  field blank containing the
         appropriate liquid used in the vials should be sent to the laboratory for analysis.
B-4                                                                                             January 2011

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           	Appendix C
            Appendix C: General CLP Sample  Collection  Guidelines
                                           VOAs in Water
        Regional guidance and/or specific Project Plan requirements will supersede the guidelines listed below.


Collect the following:

  •   At least three 40 mL glass containers with polytetrafluoroethylene (PTFE)-lined septa and open top screw-caps that
      are filled to capacity with no air bubbles, preserved to a pH of 2 with HC1, and cooled to 4°C (±2°C) immediately
      after collection. DO NOT FREEZE THE SAMPLES.

        Regular Samples           3 vials  (40 ml filled to capacity with no headspace or air bubbles)

        Regular Samples           3 vials for Sample (40 ml filled to capacity with no headspace or air bubbles)
        Requiring QC Analysis     3 vials for MS  (40 ml filled to capacity with no headspace or air bubbles)
                                  3 vials for MSD (40 ml filled to capacity with no headspace or air bubbles)

  •   If Selected Ion Monitoring (SIM)  analysis is requested, at least two additional 40 mL glass containers with PTFE-
      lined septa and open top screw-caps that are filled to capacity with no air bubbles, preserved to a pH of 2 with HC1,
      and cooled to 4°C (±2°C) immediately after collection.

        Regular Samples           3 vials for Sample (40 ml filled to capacity with no headspace or air bubbles)
        With SIM Analysis         2 vials  (40 ml filled to capacity with no headspace or air bubbles)


Test for Carbonates, Residual Chlorine, Oxidants,  and Sulfides:

  •   It is very important that samplers obtain Regional guidance when testing and ameliorating for:
      •    Carbonates;
      •    Residual chlorine (e.g., municipal waters or industrial waste waters that are treated with chlorine prior to use or
          discharge); or
      •    Oxidants.
  •   VOA samples  containing  carbonates react with the acid preservative causing effervescence  (due  to formation of
      carbon dioxide), which can cause loss of volatile analytes.
  •   Residual  chlorine present  in VOA samples can continue to  react with dissolved organic matter. This continuous
      reaction may lead to inaccurate quantitation of certain analytes present in the sample at the time of collection.
  •   Residual  chlorine and oxidants present in VOA samples can cause degradation of certain volatile analytes (e.g.,
      styrene).

Perform the following for Pre-Preserved Vials:

  1.   Pour the sample slowly down the edge of the sample vial to avoid excess aeration or agitation of the sample during
      filling.

  2.   Fill the vial completely so that a reverse (convex) meniscus is present and ensure that there are no air bubbles present
      (either in the body or especially at the top of the vial).

  3.   Place the septum on the vial so that the PTFE side is in contact with the sample, and then firmly tighten the cap.

  4.   Gently flip the vial a few times to ensure that the sample is mixed with the acid preservative.

  5.   While holding the vial upright,  gently tap the sample to check for air bubbles (either in the body or especially at the
      top of the vial).

  6.   If air bubbles are present, discard the sample  and select a new vial in which to recollect a new sample. Repeat Steps 1
      to 5 above.

  7.   Do NOT mix or composite samples for VOAs.


January 2011                                                                                           C-1

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Appendix C	

  8.   Cool sample to a temperature of 4°C (±2°C). Samplers should begin the cooling process in the field as samples are
      being collected. Double-bagged ice should be used. DO NOT FREEZE WATER SAMPLES.

  9.   Immediately transfer the vial to the sample shuttle (device that contains a "set" of VOA vials) once it has been
      collected. Do NOT allow ice to touch the vials.

Perform the Following for Empty Vials:

  1.   Rinse the vial with sample water prior to actual sample collection and preservation.

             Regions vary in their approach to pre-rinsing and/or re-using sample vials (e.g., some Regions do not
             recommend  pre-rinsing and/or re-use  of pre-cleaned containers using sample water). Be  sure  to follow
             Regional guidance.

  2.   Add  1-2 mL of acid preservative to  the  vial.  Check to ensure that the  sample you  are collecting  requires a
      preservative (follow Regional guidance).

  3.   Pour the sample slowly down the edge of the sample vial to avoid excess aeration and agitation of the sample.

  4.   Fill the vial completely so that a reverse (convex) meniscus is present and ensure that there are no air bubbles present
      (either in the body or especially at the top of the vial).

  5.   Place the septum on the vial so that the PTFE side is in contact with the sample, and then firmly tighten the cap.
  6.   Gently flip the vial a few times to ensure that the sample is mixed with the acid preservative.

  7.   While holding the vial upright, gently tap the vial to check for air bubbles (either in the body or especially at the top
      of the vial).

  8.   If air bubbles are present, discard the sample and recollect a new sample using the same sample vial. Repeat Steps 1 -
      7 above.

  9.   Check the re-collected sample for air bubbles. If air bubbles are present, additional sample water may be added to the
      vial to eliminate air  bubbles.  If there are air bubbles after three consecutive attempts to eliminate air bubbles by the
      addition of sample water, the  entire sample and sample vial should be discarded and a new sample collected.

  10.  Do NOT mix or composite samples for VOAs.

  11.  Cool sample to a temperature of 4°C (±2°C). Samplers should begin the cooling process in the field as samples are
      being collected. Double-bagged ice should be used. DO NOT FREEZE WATER SAMPLES.

  12.  Immediately transfer the vial to the sample shuttle (device which contains a "set" of VOA vials) once it has been
      collected. Do NOT allow ice to touch the vials.

Things to Remember:

  •   Samples must be shipped as  soon as possible, preferably on the same day as sample collection to avoid exceeding
      sample holding times. If overnight transit is not possible, samples should be maintained at 2  - 4°C until they are
      shipped to the laboratory.
  •   If samples are not preserved (a requirement for certain analytes), the technical holding time is shortened to 7 days.
C-2                                                                                             January 2011

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	Appendix D

             Appendix D:  Sampling Techniques and Considerations


During a sampling event, the sampler is expected to follow prescribed sampling techniques. The sampler should also be
aware of any special sampling considerations, contaminant issues, and sample compositing and mixing methods that could
affect their sampling efforts.


        Regional guidance will take precedence over any of the techniques and considerations listed below.


D. 1  General Sampling Techniques

     Information regarding  surface water, sediment, soil, and groundwater sampling can be found in many documents
     including, but not limited to, the following sources:

      •   Compendium of ERT Surface Water and Sediment Sampling Procedures, EPA/540/P-91/005
      •   Compendium of ERT Soil Sampling and Surface Geophysics Procedures, EPA/540/P-91/006
      •   Compendium of ERT Groundwater Sampling Procedures, EPA/540/P-91/007
      •   Lead in Surface Wipe Samples, NIOSH Method 9100, August 15, 1994
      •   Elements on Wipes, NIOSH Method 9102, March 15, 2003
      •   Surface Wipe Sampling Procedure,  IH75190,  Brookhaven National Laboratory, Industrial  Hygiene Group,
          February 9, 2009
      •   General Air Sampling Guidelines, US EPA ERT, SOP 2008, November 16, 1994
      •   Quality Assurance Sampling Plan for Environmental Response (QASPER) software, Version 4.1, ERT
      •   Requirements for the Preparation of Sampling and Analysis Plans', United States Army Corps  of Engineers,
          February  1, 2001, EM 200-1-3

     When working with potentially  hazardous  materials,  samplers should follow USEPA and OSHA  requirements,
     specific health and safety procedures, and DOT requirements.

D.2  Special Sampling Considerations

     Samplers  should refer  to Regionally-developed SOPs  to obtain  specific procedures for properly collecting and
     preserving samples  in  the  field. For additional guidance  regarding sampling for VOAs  in soil and water, see
     Appendices B and C. Samplers should obtain Regional guidance when testing and ameliorating for:

      •   Carbonates in VOA soil and water
      •   Residual chlorine in VOA soil and water, or cyanide water
      •   Oxidants in VOA soil and water
      •   Sulfides in cyanide

D.3  Contaminant Sampling

     Certain compounds can be detected in the parts-per-billion (ppb) and/or parts-per-trillion (ppt) range.  Extreme care
     MUST be taken to prevent cross-contamination of these samples. The following precautions should be taken when
     trace contaminants are a concern:

      •   Disposable gloves should be worn each time a different location is sampled.
      •   When collecting both surface water and sediments, surface water samples should be collected first. This reduces
          the chance of sediment dispersal into surface water, and the resulting loss of surface water sample integrity.
      •   Sampling should  occur in a progression from the least to the most contaminated area, if this information is
          known to the sampling team.
      •   Samplers should use equipment constructed of PTFE, stainless steel, or glass that has been properly pre-cleaned
          for the collection of samples for trace organic and/or inorganic analyses. Equipment constructed of plastic or
          polyvinyl chloride (PVC) should NOT be used to collect samples for trace organic compound analyses.
      •   Equipment constructed of stainless steel should NOT be used to collect samples for trace metals analyses.

D.4  Sample Compositing

     Sample compositing is a site-specific activity that must be conducted according to the SAP. Compositing is typically
     used for large  sites under investigation to improve the precision (i.e., lower the variance) of the estimated average

January 2011                                                                                         D-1

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Appendix D	

     contaminant  concentrations. Samples for VGA  analysis  should NOT be  composited  to  minimize  loss of
     VOAs/analytes.

     Composite samples consist of a series of discrete grab samples that are mixed together to characterize the  average
     composition of a given material. The discrete samples are usually of equal volume, but may be weighted to reflect an
     increased flow or volume.  Regardless, all discrete samples must be collected in an identical manner and the  number
     of grab samples forming a composite should be consistent. There are several compositing techniques  that  may be
     required such as:
      •   Flow-proportioned -  Collected proportional to the flow rate during the compositing period by either  a time-
          varying/constant volume or a time-constant/varying volume method. This technique is usually associated with
          wastewater or storm water runoff sampling.
      •   Time  - Composed of a varying number of discrete samples collected at equal time intervals during  the
          compositing period. This technique is typically used to sample wastewater and streams, and  in some air
          sampling applications.
      •   Areal - Collected from individual grab samples collected in an area or on a cross-sectional basis.  Areal
          composites are comprised of equal volumes of grab  samples where all grabs are collected in  an identical
          manner. This  technique is typically used for estimating average  contaminant concentrations  in soils or
          sediments. This technique is useful when contaminants are present in nugget form (i.e., TNT chunks, lead shot,
          etc.), thus exhibiting large differences in concentration over a small sample area.
      •   Vertical - Collected from individual grab samples but taken from a vertical cross section. Vertical composites
          are comprised of equal volumes of grab samples where all grab  samples are collected in an identical manner.
          Examples would include vertical profiles of a soil borehole or sediment columns.
      •   Volume - Collected from discrete samples whose aliquot volumes are proportional to the volume of sampled
          material. Volume composites are usually associated with hazardous waste bulking operations where the sample
          represents combined or bulked waste.
     When compositing solid or tissue samples (i.e., sediment,  soil, or sludge) for analysis of compounds present  in trace
     quantities, use a stainless steel or PTFE bowl and spatula.

D.5  Sample Mixing and Homogenizing

     Mixing of the  sample for the remaining parameters is necessary  to  create a  representative sample  media. It is
     extremely  important that  solid samples be  mixed  as thoroughly as possible to  ensure that the sample  is as
     representative as possible of the sample location. Please refer to the project-specific SAP regarding instructions on
     removal of any  extraneous materials (e.g., leaves, sticks, rocks, etc.). The mixing technique will depend on the
     physical characteristics of the solid material (e.g., particle size, moisture content, etc.). Grinding and homogenization
     of tissue is easier when it is partially frozen. The mixing container should be large enough to hold the sample  volume
     and accommodate  the procedures without spilling. Both the mixing  container  (generally a  bowl or tray)  and the
     mixing implement should  be properly decontaminated before use.  Samples  should be homogenized according to
     procedures listed in the project-specific SAP.  Table D-l provides a  short procedure for mixing a soil sample with a
     small particle size (less than 1/4 in) and filling sample containers in the field.

                           Table D-l. Mixing a Sample and Filling Sample Containers
Step
1
2
3
4
5
6
7
8
9
Action
Roll the contents of the compositing container to the middle of the container and mix.
Quarter the sample and move to the sides of the container.
Mix each quarter individually, then combine and mix OPPOSITE quarters, then roll to the middle of the
container.
Mix the sample once more, and then quarter the sample again.
Mix each quarter individually, then combine and mix ADJACENT corners, then roll to the middle of the
container. The goal is to achieve a consistent physical appearance before sample containers are filled.
Flatten piled material into an oblong shape.
Using a flat-bottomed scoop, collect a strip of soil across the entire width of the short axis and place it into a
sample container.
Repeat Step 7 at evenly-spaced intervals until the sample containers are filled.
Record the approximate quantity of each subsample in the field log book.
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                                                                    Appendix E
                     Appendix E: Sampling Checklists
                   Appendix E-1: Personnel Preparation Checklist
                                  (Page 1 of 1)
Personnel Briefing
1 . Did you review sampling team responsibilities and identify individual(s) responsible
for corrective actions?
2. Did you ensure that you have met the appropriate personal safety and protection
requirements?
3. Did you identify sampling locations and receive permission to access them, as
appropriate?
4. Did you contact the appropriate utility companies PRIOR to the start of sampling?
Bv law. utility companies must be contacted prior to the start of
;y'"n digging/sampling so that any underground utilities (gas lines, water
' tJ lines, electrical lines, etc.) can be marked. A list of one-call centers for
each state may be found at: http://www.call81 1 .com.
5. If sampling on private property, do you have sample receipts to provide to the
property owner for all samples taken and removed from the property?
6. Have you determined the number and type of samples to be collected?
7. Did you review sample collection methods?
8. Have you reviewed sample container requirements?
9. Did you review decontamination requirements, procedures, and locations?
10. Did you determine holding times and conditions?
1 1 . Did you determine Performance Evaluation (PE) and Quality Control (QC) sample
requirements?
12. Have you obtained shipping cooler temperature blanks, if required?
1 3 . Did you review sample label and tag requirements?
14. Did you review Traffic Report/Chain of Custody (TR/COC) record and custody seal
requirements?
15. Have you obtained the laboratory name, shipping addresses, and telephone number?
1 6 . Did you review cooler return instructions?
17. Have you obtained shipping company information (name, telephone number,
account number, pickup schedule)?
18. Have you obtained shipping schedules?
19. Did you review shipment reporting requirements and the appropriate contact names
and telephone numbers for reporting?
20. Have you included any sampler comments regarding sampling issues (e.g., low
volumes, matrix, suspected concentrations based on field measurements)?
Yes




















No




















Comments




















January 2011
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Appendix E
                    Appendix E-2: General Sample Collection Checklist
                                       (Page 1 of 1)
General Sample Collection
1. Did you identify and mark the sampling location with buoys, flags, or stakes
according to the sampling plans, maps, and grids?
2. If the sampling location is inaccessible, did you contact the appropriate field or
Regional personnel for instructions?
3. Did you use the correct sampling equipment?
4. Did you follow the correct decontamination procedures?
5 . Did you follow the correct collection procedures?
6. Did you use the correct sample containers for each sample collected?
7. Did you use certified clean containers for all samples? Are certificates kept on
record?
8. Did you use certified clean water for all field, trip, equipment and rinsate blanks?
Are certificates kept on record?
9. Did you collect the correct volume for each sample?
10. Did you collect the correct type of sample, including primary samples and Quality
Control (QC) samples?
1 1 . Did you properly preserve each sample collected?
12. Did you correctly document and label each sample with all necessary information?
Under no circumstances should the site name appear on any
Jrj*"4* documentation being sent to the laboratory, unless the laboratory is a
ijjfj Regional USEPA laboratory. Then the Region copy of the TR/COC shall
be sent to the USEPA laboratory.
13. If sampling on private property, did you provide a sample receipt to the owner of the
property for all samples taken and removed from the property?
Yes













No













Comments













E-2
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                                                                             Appendix E
                    Appendix E-3: Completing Field Logbook Checklist
                                       (Page 1 of 1)
Completing Field Logbook
1 . Did you use waterproof ink when writing in the field logbook?
2. Did you document sampling project information such as:
X Project name, ID, and location
X Names of samplers
X Geological observations, including maps
X Atmospheric conditions
X Field measurements
X Sampling dates, times, and locations?
Under no circumstances should the site name appear on any
JrT% documentation being sent to the laboratory, unless the laboratory is a
\^/ Regional USEPA laboratory. Then the Region copy of the TR/COC shall
be sent to the USEPA laboratory.
3. Did you record sampling activity information such as:
X Sampling dates and times
X Sample identifications
X Sample matrices
X Sample descriptions (e.g., odors and/or colors)
X Number of samples taken
X Sampling methods/equipment
X Description of QC samples?
4. Did you document any and all deviations from the sampling plan?
5. Did you document any and all difficulties in sampling and/or any unusual
circumstances?
6. Were all errors corrected by crossing a line through the error, initialing the error,
dating the error, and then adding the correct information?
Yes






No






Comments






January 2011
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Appendix E
              Appendix E-4: Completing Handwritten Sample Labels Checklist
                                      (Page 1 of 1)
Completing Handwritten Sample Labels
1. Did the Region provide CLP Sample numbers and SMO-assigned CLP Case
numbers?
2. If additional CLP Sample numbers were needed, did you contact the appropriate
Regional personnel?
3. Were the CLP Sample numbers and SMO-assigned CLP Case numbers on the
labels correct? Organic CLP Sample numbers begin with the Regional letter code,
followed by letters and numbers. Inorganic CLP Sample Numbers begin with "M,"
followed by the Regional letter code, and then letters and numbers.
4. Were samples uniquely numbered and designated to only one sample?
Samples collected for total metal and dissolved metal analyses must
IJ^ J receive separate, unique, CLP Sample numbers.
5. Were Quality Control (QC) samples numbered accordingly?
6. Were the specific requirements followed for total and dissolved metals analysis,
QC and Performance Evaluation (PE) samples, and SW-846 Method 5035A?
7. Were all temperature blanks labeled with "TEMPERATURE BLANK"?
8. Was a sample label containing the CLP Sample number, SMO-assigned CLP Case
number, location, concentration, preservative, and the analysis, attached to each
sample bottle or container as the sample was collected?
Under no circumstances should the site name appear on any
ff*\ documentation being sent to the laboratory, unless the laboratory is a
ijyj Regional USEPA laboratory. Then, the Region copy of the TR/COC
record shall be sent to the USEPA laboratory.
9. Was clear tape placed over the sample labels to protect the labels from moisture
and to help the labels adhere to the sample bottle?
Use only CLEAR tape over the sample labels and avoid wrinkles in the
l^jj tape and the sample labels.
10. Were all errors corrected by crossing a line through the error, initialing the error,
dating the error, and then adding the correct information?
Yes










No










Comments










E-4
January 2011

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                                                                           Appendix E
      Appendix E-5: Completing Handwritten Sample Tags & Custody Seals Checklists
                                      (Page 1 of 1)
Completing Handwritten Sample Tags
1 . Was waterproof ink used on the sample tags?
2. If Regionally required for individual sample containers, was the project code on the
sample tag completed?
3. Was the station number on the sample tag completed?
4 Was the date filled in using the format MM/DD/YYYY?
5 . Was the time of sample collection indicated in military time format HH:MM?
6. Was the box checked indicating composite or grab sample?
7. Was the station location on the sample tag completed?
8. Did you indicate whether or not the sample was preserved by checking "yes" or "no?"
9. Was the appropriate analysis indicated on the sample tag?
10. Were the appropriate CLP Sample number and SMO-assigned CLP Case number
indicated and cross-referenced with the numbers on the sample label?
1 1 . Did you sign the sample tags?
12 Did you attach the sample tag to the neck of the sample bottle with string, stretch
string, or wire?
C I J ^° NOT use wire to attach a sample tag to a metal sample.
13. Were all errors corrected by crossing a line through the error, initialing the error,
dating the error, and then adding the correct information?
Completing Custody Seals
1 . Did you sign and date the custody seal?
2. Did you attach a completed custody seal to the sample bottle, container, or plastic
bag, placing the seal over the cap or lid of each sample bottle or container or on the
bag opening such that it will be broken if the sample bottle, container, or bag is
opened or tampered with?
3. As appropriate, did you attach the completed custody seal to the sample shipping
container or cooler, placing the seal such that it will be broken if the container or
cooler is opened or tampered with?
4. Were all errors corrected by crossing a line through the error, initialing the error,
dating the error, and then adding the correct information?
Yes













Yes




No













No




Comments













Comments:




January 2011
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Appendix E
                    Appendix E-6: Packing Sample Container Checklist
                                       (Page 1 of 1)
Packing Sample Container
1.
2.
3.
4.
5.
6.
1.
Did you follow all State, Federal, Department of Transportation (DOT), and
international Air Transportation Association (IATA) regulations governing the
packaging of environmental and hazardous samples?
,.,.!5"k/j If samples contain methanol preservation (e.g., samples to be analyzed
* (*»n J by SW-846 Method 5035A), refer to the packaging instructions in

Were all CLP Sample numbers, SMO-assigned CLP Case numbers, analyses, labels,
tags, and custody seals attached to the correct sample containers?
Is modified analysis indicated if requested?
Was an inventory conducted of CLP Sample numbers, SMO-assigned CLP Case
numbers, analyses, and containers, and verified against the TR/COC records?
Were the correct number and type of Performance Evaluation (PE) and Quality
Control (QC) samples collected?
Were all sample containers sealed in clear plastic bags with the sample label and tag
visible through the packaging?
Were all soil/sediment samples known or suspected to contain dioxin securely
enclosed in metal cans (e.g., paint cans) with the lids sealed?
8. Was suitable absorbent packing material placed around the sample bottles or
containers?
9.
Were the outsides of metal containers labeled properly with the CLP Sample
number, SMO-assigned CLP Case number, and the analysis of the sample inside?
Yes









No









Comments









E-6
January 2011

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                                                                               Appendix E
                    Appendix E-7: Packing Shipping Container Checklist
                                       (Page 1 of 1)
Packing Shipping Container
1 . Were you shipping samples in a clean waterproof metal or hard plastic ice chest or
cooler in good condition?
2. Were all non-applicable labels from previous shipments removed from the container?
3. Were all inside and outside drain plugs closed and covered with suitable tape (e.g.,
duct tape)?
4. Was the inside of the cooler lined with plastic (e.g., large heavy-duty garbage bag)?
5. Was the lined shipping cooler packed with noncombustible absorbent packing
material?
6. Were sample containers placed in the cooler in an upright position not touching one
another?
7. Was a sample shipping cooler temperature blank included in the cooler?
8. Did the documentation in the cooler only address the samples in that cooler?
9. Was the site name absent from all documentation?
Under no circumstances should the site name appear on any
jP|*<% documentation being sent to the laboratory, unless the laboratory is a
iJJP Regional USEPA laboratory. Then the Region copy of the TR/COC shall
be sent to the USEPA laboratory.
10. Was there sufficient packing material around and in between the sample bottles and
cans to avoid breakage during transport?
11. If required, was double-bagged ice placed on top and around sample bottles to keep
the samples cold at 4°C (± 2° C)?
Cl J ^° not Pac^ l°ose ice mto me co°ler-
12. Was the top of the plastic liner fastened and secured with tape?
13. Was a completed custody seal placed around the top of the fastened plastic liner (if
required by the Region)?
14. Were all sample documents enclosed within the cooler (e.g., TR/COC record and
cooler return instructions) in a waterproof plastic bag?
15. Was the plastic bag, containing the documentation, taped to the underside of the
cooler lid?
16. Were cooler return instructions and airbills taped to the underside of the cooler lid?
17. Was the return address of the cooler written with permanent ink on the underside of
the cooler lid?
18. Was tape placed around the outside of the entire cooler and over the hinges?
19. Were the completed custody seals placed over the top edge of the cooler so the cooler
cannot be opened without breaking the seals?
20. Was the return address label attached to the top left comer of the cooler lid?
21. Were instructional labels attached to the top of the cooler, as necessary (e.g., "This
End Up," "Do Not Tamper With," or "Environmental Laboratory Samples")?
22. Have all US D.O.T. regulations been met for the shipment when shipping hazardous
samples?
23. If shipping samples containing methanol as a preservative (e.g., samples to be
analyzed by SW-846 Method 5035A), was a label used to indicate methanol, the
United Nations (UN) identification number for methanol (UN 1230), and Limited
Quantity?
Yes























No























Comments























January 2011
E-7

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Appendix E
                Appendix E-8: Shipping & Reporting CLP Samples Checklist
                                      (Page 1 of 1)
Shipping CLP Samples
1. Did you follow all State, Federal, Department of Transportation (DOT), and
International Air Transportation Association (IATA) regulations governing the
shipment of environmental and hazardous samples?
2. Was a separate airbill filled out for each cooler being shipped?
3. Was the airbill filled out completely, including correct laboratory name, address, and
telephone number, identification of recipient as "Sample Custodian," and appropriate
delivery option (e.g., overnight or Saturday)?
4. Was the completed airbill attached to the top of the cooler with the correct laboratory
address?
5. If more than one cooler was being shipped to the same laboratory, were they marked
as"l of 2," "2 of 2," etc.?
6. Were the samples being shipped "overnight" through a qualified commercial carrier?
Reporting CLP Samples
1 . Did you contact the RSCC (or designee) or the Contract Laboratory Program Sample
Management Office (SMO) on the same day samples were shipped?
2. If the samples were shipped after 5:00 PM Eastern Time (ET), were they reported to
the RSCC (or designee) or to SMO by 8:00 AM ET the following business day?
3 . Did you notify the RSCC (or designee) or SMO so that SMO will receive the delivery
information by 3:00 PM ET on Friday for sample shipments that will be delivered to
the laboratory on Saturday?
4. Did you provide the RSCC (or designee) or SMO with:
X Your name, phone number, and Region number;
X Case Number of the project;
X Modified Analysis Number, if requested;
X Exact number of samples, matrix(ces), concentration(s), and type of analysis;
X Laboratory(ies) to which the samples were shipped;
X Carrier name and airbill number;
X Date of shipment;
X Date of next shipment; and
X Any other information pertinent to the shipment?
Yes






Yes




No






No




Comments:






Comments:




E-8
January 2011

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                                     	Appendix F

                                     Appendix F: Glossary
Analyte ~ The element,  compound, or ion that is  determined in an analytical procedure;  the substance or chemical
constituent of interest.

Analytical Services Branch (ASB) - Directs the Contract Laboratory Program (CLP) from within the United States
Environmental Protection Agency's (USEPA's) Office of Superfund Remediation and Technology Innovation (OSRTI) in
the Office of Solid Waste and Emergency Response (OSWER).

Aroclor - Fob/chlorinated biphenyls (PCBs) or a class of organic compounds with 1 to 10 chlorine atoms attached to
biphenyl and a general chemical formula of Ci2H10-xClx. PCBs, commercially produced as complex mixtures containing
multiple isomers at different degrees of chlorination, were marketed in North America under the trade name Aroclor.

Case ~ A finite, usually predetermined, number of samples collected over a given time period from a particular site. Case
numbers are assigned by the Sample Management Office (SMO). A Case consists of one or more Sample Delivery Groups
(SDGs).

Comprehensive  Environmental Response, Compensation,  and Liability  Act (CERCLA)  ~ First  authorized by
Congress in December 1980, and amended in 1986, CERCLA provided broad Federal authority to respond directly to the
release or possible release or hazardous substances that may endanger human health or the environment.  CERCLA also
established a Trust Fund  to provide for cleanup when no responsible party could be identfied; hence, CERCLA  is
commonly referred to as "Superfund."

Contract Laboratory Program (CLP) - A national program of commercial  laboratories under contract to support the
USEPA's nationwide efforts to clean up designated  hazardous waste sites by providing a range of chemical analytical
services to produce environmental  data of known  and documented quality.  This program is directed  by  USEPA's
Analytical Services Branch (ASB).

Contract Laboratory Program Project Officer (CLP PO) - Monitors technical performance of the contract laboratories
in each Region.

Contract Laboratory Program Sample Management Office (CLP SMO) ~ A contractor-operated facility operated
under the  CLP,  awarded  and administered by the  USEPA, which provides  necessary management, operations, and
administrative support to the CLP. SMO coordinates and schedules sample analyses, tracks sample shipments and analyses,
receives and tracks data for completeness and compliance, and processes laboratory invoices.

Custody Seal - An adhesive label or tape that is used to seal a  sample bottle or container that maintains chain-of-custody
and that will break if the sample bottle or container is opened or tampered with.

Cyanide (Total) ~ Cyanide ion and complex cyanides converted to hydrocyanic acid (HCN) by reaction in a reflux system
of a mineral acid in the presence of magnesium ion.

Data Quality Objective (DQO) - The requirements established to maintain the quality of the data being collected.

Data Validation ~ Data  validation is based on Region-defined criteria and limits, professional judgment of the data
validator, and (if available) the Quality Assurance Project Plan (QAPP) and Sampling and Analysis Plan (SAP).

Duplicate  ~ Sample required by the laboratory's contract to check the accuracy and precision of inorganic analyses. It is a
second aliquot of the same sample to determine the precision of the method.

Equipment Blank ~ A sample used to check field decontamination procedures. See Field Blank.

Field Blank ~ Any blank sample that is submitted from the field. Each field blank is assigned its own unique USEPA
Sample number.  A field blank checks for cross-contamination during sample  collection,  sample  shipment, and in the
laboratory. A field blank includes trip blanks, rinse blanks, equipment blanks, etc.

Field Duplicate ~ Checks reproducibility of laboratory and field procedures and indicates non-homogeneity.

Field  Operations and  Record  Management  System (FORMS)  II Lite ~  A stand-alone, Windows-based software
application that enables samplers to  automatically create and generate sample documentation both prior to and during a
sampling event.

Field QC Sample ~ Used  to detect for contamination or error in the field.
January 2011                                                                                          F-1

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Appendix F	

Field Sample - Primary sample material taken out in the field from which other samples,  such as duplicates or split
samples are derived. A field sample can be prepared in the field and sent for analysis in one or multiple containers, and is
identified by a unique USEPA Sample number.

Field Sampling Plan (FSP) - Developed to outline the actual steps and requirements pertaining to a particular sampling
event, and explains, in detail, each component of the event to all involved samplers.

Holding Time ~ The elapsed time expressed in hours, days, or months from the date of collection of the sample until the
date of its analysis.

    Contractual -  The maximum lengths of time that the CLP laboratory can hold samples prior to extraction and/or
    analysis, and are described in the CLP analytical services Statements of Work (SOWs).

    Technical ~ The maximum lengths of time that  samples may be held from time of collection to time of preparation
    and/or analysis and still be considered valid.

Laboratory Blank - See Method Blank.

Laboratory Duplicate - A sample required by the laboratory's contract to check the precision of inorganic analyses.

Laboratory QC Sample ~ An additional volume of an existing sample, as required by the laboratory's contract, used to
detect contamination or error in the laboratory's practices.

Matrix - The predominant material of which a sample to be  analyzed is composed.

Matrix Spike (MS) -  Sample required by  the laboratory's contract to  check the  accuracy of organic and  inorganic
analyses. It is an aliquot of a sample (water or soil) that is fortified (spiked) with known quantities of a specific compound
and subjected to the entire analytical procedure. See Matrix Spike Duplicate.

Matrix Spike Duplicate (MSD) ~ Sample required  by the laboratory's contract to check the accuracy and precision of
organic analyses.  It is a second aliquot of the same matrix as  the Matrix  Spike (MS) that  is spiked to determine the
precision of the method.  See Matrix Spike.

Method Blank ~ An analytical control consisting of all reagents, internal standards and surrogate standards [or System
Monitoring Compounds  (SMCs) for volatile organic  analysis], that is carried throughout the entire analytical procedure.
The method blank is used to  define the level of laboratory, background, and reagent contamination, also referred to as
laboratory blank when defining the level of laboratory contamination.

Modified Analysis - A change in the technical requirements  that fall outside the scope of the Statement of Work (SOW).

Performance Evaluation  (PE) Sample ~ A sample of known composition provided by the USEPA for contractor
analysis. Used by USEPA to evaluate contractor performance.

Pesticides ~  Substances intended to repel, kill, or  control any species designated a "pest," including weeds, insects,
rodents, fungi, bacteria,  and  other organisms. Under the  CLP, only organochlorine pesticides are analyzed  (e.g., DDT,
Dieldrin, Endrin, etc.).

Polychlorinated Biphenyls (PCBs) ~ A group of toxic, persistent chemicals  used in electrical transformers and capacitors
for insulating purposes, and in gas pipeline  systems as a lubricant. The sale and new use of PCBs were banned by law in
1979.

Quality Assurance  (QA) ~ An  integrated system of management activities  involving  planning,  implementation,
assessment, reporting, and quality improvement to ensure that a process, item, or service is of the type and quality needed
and expected by the customer.

Quality Assurance Project  Plan (QAPP)  ~ Document written to meet requirements outlined  in the document EPA
Guidance for Quality Assurance Project Plans (EPA QA/R-5).  Prepared in advance of field activities and used by samplers
to develop any subsequent plans such as the Sampling Analysis Plan (SAP) or the Field Sampling Plan (FSP).

Quality Control (QC)  ~ The overall system of technical  activities that  measures the attributes and performance of a
process, item, or service against  defined standards to verify  that they meet the stated requirements established by the
customer; operational techniques and activities that are used to fulfill requirements for quality.

Regional Sample Control Center (RSCC) Coordinator ~ In most Regions, coordinates sampling efforts and serves as
the central point-of-contact for sampling questions  and  problems. Also  assists in coordinating  the level  of Regional
sampling activities to correspond with the monthly projected  demand for analytical services.

Regional Site Manager - Coordinates the development of data quality objectives and oversees  project-specific remedial or
removal contractors, State officials, or private parties conducting site sampling efforts.

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	Appendix F

Rinse Blank - A sample used to check decontamination procedures. Also see Field Blank.

Routine Analytical Service (RAS) ~ The standard inorganic and organic analyses available through the CLP.

Sample - A discrete portion of material to be analyzed that is contained in single or multiple containers, and identified by a
unique Sample number.

Sample Delivery Group (SDG) - A unit within a sample Case that is used to identify a group of samples for delivery. An
SDG is defined by the following, whichever is most frequent:
  •   Each Case of field samples received; or
  •   Each 20 field samples (excluding PE samples) within a Case; or
  •   Each 7 calendar day period (3 calendar day period for 7-day turnaround) during which field samples in a Case are
      received (said period beginning with the receipt of the first sample in the SDG).
In addition, all samples and/or sample analyses assigned to an SDG must have been scheduled under the same contractual
turnaround time. Preliminary Results have no impact on defining the SDG. Sample may be assigned to SDGs by matrix
(e.g., all soil samples in one SDG, all water samples in another) at the discretion of the laboratory.

Sample Label ~ An identification label attached to a sample bottle or container to identify the sample.

Sample Number ~ A unique number used to identify and track a sample. This number can be recorded on a sample label
or written on the sample bottle or container using indelible ink.

Sample Tag ~ A tag attached to a sample that identifies the sample and maintains chain-of-custody.

Sampling Analysis Plan (SAP) - A document that explains how samples are to be collected and analyzed for a particular
sampling event.

Scribe ~ A stand-alone Windows-based desktop application that samplers can use to automatically create and generate
sample documentation prior to and during sampling events.

Semivolatile Organic Analyte  (SVGA) ~ A compound amenable to analysis by extraction of the sample using an organic
solvent.

Standard Operating Procedure  (SOP) - A written document that details the method for an operation, analysis, or action
with thoroughly prescribed techniques and steps,  and that is officially approved as the methods for performing certain
routine or repetitive tasks.

Statement of Work (SOW) ~ A document that specifies how laboratories analyze samples under a particular Contract
Laboratory Program (CLP) analytical program.

Superfund ~  The program operated under the legislative authority of the Comprehensive  Environmental Response,
Compensation, and Liability Act (CERCLA) and Superfund Amendments and Reauthorization Act (SARA), that funds and
carries out USEPA removal and remedial  activities at hazardous waste sites. These activities include  establishing the
National Priorities List (NPL), investigating sites for inclusion on the list, determining their  priority, and conducting and/or
supervising cleanup and other remedial actions.

Superfund  Amendments  and  Reauthorization  Act (SARA)  ~  The  1986  amendment to the  Comprehensive
Environmental Response, Compensation, and Liability Act (CERCLA).

Traffic Report/Chain of Custody (TR/COC) Record ~ A record that is functionally  similar to  a packing slip that
accompanies a shipment of goods. Used as physical evidence of sample custody and functions as a permanent record for
each sample collected.

Trip Blank ~ A sample used to  check for  contamination during sample handling and shipment from field to laboratory.
Also see Field Blank.

Volatile  Organic  Analyte (VGA) - A compound amenable to  analysis by  the  purge-and-trap technique. Used
synonymously with the term purgeable compound.
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                                                              Appendix G
             Appendix G: FORMS II Lite Analysis Codes
Analysis Name
Aluminum
Antimony
Arsenic
Barium
Beryllium
Cadmium
Calcium
Chromium
CLP TAL Total Metals ICP-MS
CLP TAL Total Metals/ICP-AES
CLP TCL Aroclors
CLP TCL Pesticides
CLP TCL Semivolatiles
CLP TCL Semivolatiles-SIM
CLP TCL Trace Volatiles
CLP TCL Trace Volatiles-SIM
CLP TCL Volatiles
Cobalt
Copper
Cyanide
Iron
Lead
Magnesium
Manganese
Mercury
Nickel
Potassium
Selenium
Silver
Sodium
Thallium
Vanadium
Zinc
Abbreviation
Al
Sb
As
Ba
Be
Cd
Ca
Cr
ICP/MS
ICP-AES
CLPARO
CLP PEST
CLP SVGA
CLP SVSIM
CLP TVOA
CLP TVSIM
CLP VGA
Co
Cu
CN
Fe
Pb
Mg
Mn
Hg
Ni
K
Se
Ag
Na
Tl
V
Zn
January 2011
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                                                                                       Appendix H
                     Appendix H: Scribe CLP Analysis Codes
Analysis Name
CLP Aluminum
CLP Antimony
CLP Arsenic
CLP Barium
CLP Beryllium
CLP Cadmium
CLP Calcium
CLP Chromium
CLP Cobalt
CLP Copper
CLP Iron
CLP Lead
CLP Magnesium
CLP Manganese
CLP Nickel
CLP Potassium
CLP Selenium
CLP Silver
CLP Sodium
CLP TAL Dissolved Metals
CLP TAL Total Metals
CLP TAL Total Metals (No Hg)
CLP TAL Total Metals and Cyanide
CLP TAL Total Metals ICP/MS
CLP Thallium
CLP Vanadium
CLP Zinc
CLP TCL Pesticide/PCBs
CLP TCL Semivolatiles
CLP TCL Semivolatiles and Pesticides/PCBs
CLP TCL Volatiles
Abbreviation
Al
Sb
As
Ba
Be
Cd
Ca
Cr
Co
Cu
Fe
Pb
Mg
Mn
Ni
K
Se
Ag
Na
DM
TM
TM (No Hg)
TM/CN
ICP/MS
Tl
V
Zn
PEST
BNA
BNA/PEST
VGA
            The Scribe CLP Analysis Codes provided may cause a delay in processing at the CLP laboratories
            based on interpretation.  Samplers are advised to use the analysis codes provided in Appendix
            G: FORMS II Lite Analysis Codes for CLP analysis.

            Scribe users are advised to contact the ERT Software Support Help Desk at 800-999-6990 for
            assistance in adding the custom analysis onto Scribe, please  contact or refer to the following Web
            site:

                                      http://www.epaosc.orq/Scribe
January 2011
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