U.S. EPA Environmental Technology Verification (ETV) Program
                  Advanced Monitoring Systems (AMS) Center
                  Water Stakeholder Committee Teleconference
                            Wednesday, May 27, 2009
  Stakeholder Committee Members:
  Joel Allen, EPA
  John Carlton, Alabama Dept. of Environmental Management (retired)
  Tom Gargan, U.S. Army Center for Environmental Health Research
  Christine Kolbe, Texas Commission on Environmental Quality
  Max Lee, Dow Chemical
  Marty Link, Nebraska Department of Environmental Quality
  Alan Mearns, Hazardous Materials Response Division, National Oceanic and
    Atmospheric Administration (NOAA)
  Vito Minei,  Suffolk County Department of Health Services
  Rick Sakaji, East Bay Municipal Utility District
  Ken Wood, DuPont Corporate Environmental Engineering Group

  Dick Burrows, U.S. Army Center for Health Promotion and Preventive Medicine
  Will Myers, Nebraska Department of Environmental Quality
  Dave Schumacher, Nebraska Department of Environmental Quality

  ETV AMS Center Staff:
  Amy Dindal, Battelle
  Teresa Harten, EPA
  Michelle Henderson, EPA
  Ryan James, Battelle
  John McKernan, EPA
  Rachel Sell, Battelle

Rachel Sell, Battelle AMS Center Stakeholder Coordinator, welcomed committee stakeholders
and AMS Center staff, took roll call of the participants in the teleconference, and provided an
overview of the agenda.

ETV Updates and AMS Center News
Dr. John McKernan, AMS Center EPA Project Officer, announced that the ETV AMS Center
has received support from the EPA Environmental Technology Council (ETC) to fund four
proposals for verification of water monitoring technologies:

•  Evaluation of Nitrate Sensors for Groundwater Remediation Monitoring
•  Pathogen Monitors for E. coli and Total Coliforms in Water
•  Testing Toxic Blue-Green Algae for Microcystins in Freshwater Sources
•  Monitoring Technologies for Measuring Stored Carbon Dioxide from Sequestration
These technology categories have been priorities for the AMS Center stakeholders. All four
projects will involve AMS testing of novel and potentially cost-effective monitoring
technologies to provide results in short time frames. The four proposals were selected from more
than 20 monitoring technology proposals submitted to the ETC for consideration.
There already are some vendors  interested in testing.  Oklahoma Department of Agriculture has
sites for the nitrate sensor evaluation, Nebraska Department of Environmental Quality (NDEQ)
for microcystins, and EPA Region 7 for carbon sequestration.

Discussion of New Technology Categories
Dr. Ryan James, Battelle, provided an update on microcystins andE.coli monitoring
technologies. The stakeholders followed along with the presentation on slides received before
the teleconference.  Dr. James covered:
•  Immunoassay Test Kits for Microcystins
   It has been four years since the stakeholders expressed interest in this approach, and now,
   with new funding, testing will be able to start this summer. We will work with Marty Link
   and Dave Schumacher at Nebraska DEQ, which has an  extensive sampling program this
   summer. Marty Link (Nebraska DEQ) said that an incident in 2004, in which several dogs
   died, prompted testing of beach water which showed the presence of microcystins.  Now the
   state samples 49 beaches per week.  Samples are collected on Mondays and Tuesdays, then
   frozen/thawed/run using an Abraxis test kit, with results available by the end of the week.
   We will piggyback on this program,  and probably do tests at their facilities in Nebraska.
   Marty mentioned that their microcystin  health alert level is 20 parts per billion (ppb). Vito
   Minei (Suffolk County Department of Health Services) asked whether this information was
   posted at beaches? Marty  replied that it is.
•  Possible Vendors
   Dr. James is talking with vendors and hopes to firm their commitments in the next few
   weeks. Discussions are on-going with Abraxis,  Beacon  Analytical, and Strategic Diagnostics.
•  Experimental Plan
   Plans have been outlined for both the Laboratory Testing and Environmental Sample Phases.
   The major concern is the LC-MS reference analyses, which are extremely expensive which
   will drive the laboratory testing effort.
•  Schedule
   Once peer review of the draft test/quality assurance plan (TQAP) has been completed, the
   TQAP can be approved. We  are anticipating that testing will proceed in August.
•  Coordination with E. coli and Total Coliform Detection Verification Test
   The AMS Center is considering pulling this into the microcystin immunoassay test. There is
   efficiency in having two similar tests run at the  same time, and takes advantage of co-funding
   and partnering. Right now, there are two technology vendors interested in this test: B2P and
   Early Warning.

Tom Gargan (U.S. Army) asked about the principles behind the B2P technology for E. coli and
Total Coliform monitoring.  Dr. James said there is a color change after filtration and incubation
in the presence of violet red bile agar (VRBA) that originates in the cap of the sample container.
Dr. Gargan also observed that Early Warning picks up both viable and nonviable bacteria.
In reference to the E. coli detection tests, Rick Sakaji (East Bay Municipal Utility District) asked
if the testing will consider EPA's Alternate Test Procedure (ATP) protocols?  Teresa Harten
(EPA) said that she and John McKernan (EPA) recently met with EPA's Office of Ground Water
and Drinking Water (OGWDW) to discuss changes that are in process with the Total Coliform
Rule (TCR). Stakeholders commented that testing would be most useful if is focused more
towards drinking water and the TCR, rather than recreational water.
Vito Minei said that Suffolk Co. is interested in both microcystin and E. coli.  His group is
alarmed at the prevalence of microcystin, and monitors more than 200 beaches.  He also asked
about bacteroides testing. Tom Gargan said that their bacteroides program was no longer active,
and had moved to E. coli and coliform detection in freshwater.
With regards to peer reviewers for this test, Rick Sakaji offered to review the E. coli documents,
provided there is a focus on drinking water. Vito Minei offered to serve as a peer reviewer for
the microcystin test. It was noted that Robin Oshiro of EPA's ATP program would potentially
be interested in serving as a peer reviewer as well and her interest would need to be confirmed.

Testing Results for the BioTector TOC Analyzer
Max Lee, Dow, gave a presentation on why Dow Chemical chooses to use the BioTector. The
stakeholders were able to follow the presentation on slides received before the teleconference.
He covered the benefits of this analyzer, performance testing conducted by Dow, and an
evaluation of instrument cost as compared to other systems.
Update on Current Verification Tests
Amy Dindal, Battelle,  gave a comprehensive update on several on-going verification tests. The
stakeholders were able to follow the presentation on slides received before the teleconference.
She covered:
•  ELISA Test Kits for Endocrine Disrupting Compounds (EDCs) in Water
•  International ETV  - Passive Groundwater Samplers
•  Nitrate Sensors for Groundwater Monitoring
•  Cryptosporidium Monitor
•  Balloon Remote Sensing of Mixing Zones

Vito Minei offered to serve as a peer reviewer for the Nitrate Sensors test, and offered a New
York site for field testing.

Vendor Inquiries
Amy Dindal led the discussion on the following technologies:
•  Chemical Oxygen Demand
   AquaDiagnostics has lost interest.  However, Machery-Nagel, a German vendor that uses
   spectrophotometric treatment of wastewater, is interested in joint testing with the
   international ETV program. One stakeholder commented that an instrument based on
   chromium chemistry can't be used in Europe.

•  Turbidimeters
   The ATP program has expressed interest in developing a joint (ETV/ATP) protocol for
   testing this category of technologies that could be use to generate data for method
   compliance. An existing ETV TQAP for on-line turbidimeters prepared by Battelle would be
   used as a starting point (see http://www.epa.gov/nrmrl/std/etv/vt-ams.htmltfTurbidimeters)
•  Vacuum distillation GC-MS
   Vendor interest in looking at this technology for 1,4-dioxane in water, wastewater, and
•  Pipe locating technology
   Technology to ascertain depth and location of underground pipes of water/wastewater. Alan
   Mearns (NOAA) asked that more information be sent about this technology.
•  Passive groundwater samplers
   At least two vendors have expressed interest in international testing of such samplers.
•  ELISA for PAHs
   This was an inquiry from Alan Mearns. PAHs have been found in oysters, shellfish, and fish
   as an environmental indication of contamination. Should the AMS Center consider
   evaluating ELISA test kits for PAHs (such as the RaPID assay being sold by Strategic

Stakeholders: what's on your radar screen? - Rachel Sell
Ken Wood (DuPont) inquired about on-line monitoring for extremely low levels (parts per
trillion) of mercury in water. Ms. Dindal  replied that all of the mercury monitoring that has been
done by the AMS Center has been in air.  P  S Analytical and Arizona Instruments are some
vendors who have applicability to water.

Recap of Priorities, Action Items,  and Next Meeting - Rachel Sell
•  The next teleconference will take place some time in September/October
•  Microcystins reviewers—Vito Minei and Marty Link
•  E.Coli reviewers - Rick Sakaji and Robin Oshiro (interest to be confirmed)
•  Nitrate sensors—Vito Minei has agreed  to be a peer reviewer; also offered test site if
•  Cryptosporidium monitor—Panel discussion in next 4-6 months. Rick Sakaji/Lisa
   Olsen/Peter Tennant/Geoff Scott interested in serving on technical panel.
•  Balloon remote sensing—Panel discussion in next 4-6 months. Lisa Olsen and Peter Tennant
   are interested in serving on technical panel.
•  AMS Center staff will send out information to stakeholders for the following:
   — COD monitor
   -vacuum distillation
   -pipe locator
   -ELISA for PAHs