ENVIRONMENTAL TECHNOLOGY VERIFICATION
Canada
DANETV
£EPA
WATER* ENVIRONMENT* HEALTH
Baiteiie
The Business of Innovation
ETV Joint Verification Statement
TECHNOLOGY TYPE: Handheld luminometer
APPLICATION:
PRODUCT NAME:
COMPANY:
ADDRESS:
WEB SITE:
E-MAIL:
Toxicity testing of effluent wastewater.
ECLOX Handheld Luminometer with LUMIStherm
Thermoblock and LUMISsoft4 Software
HACH-LANGE GmbH
Willstatterstrasse 11 PHONE: +49 211 5288 0
D-40549 Diisseldorf, Germany
www.hach-lange.de
elmar.grabert@hach-lange.de
Testing and verification of a handheld luminometer for detecting toxicity of wastewater effluent
was conducted as a joint verification project with the Danish Centre for Verification of Climate and
Environmental Technologies (DANETV), ETV Canada, and the United States Environmental
Protection Agency (U.S. EPA) Environmental Technology Verification Program (ETV). The testing
and verification satisfied the requirements of the Canadian ETV program, U.S. ETV program and
the ETV scheme currently being established by the European Union (EU ETV).
Environment Canada established the Canadian ETV program to provide credible information for
promoting the commercialization and market deployment of new environmental technologies, thus
helping to address environmental challenges efficiently, effectively and economically. Information
on the Canadian ETV program is available at www.etvcanada.ca.
Page 1 of 6
-------
The U.S. ETV program was established to facilitate the deployment of innovative or improved
environmental technologies through performance verification and dissemination of information.
The goal of U.S. ETV is to further environmental protection by accelerating the acceptance and use
of improved and cost-effective technologies. U.S. ETV seeks to achieve this goal by providing
high-quality, peer-reviewed data on technology performance to those involved in the design,
distribution, financing, permitting, purchase, and use of environmental technologies. Information
and U.S. ETV documents are available at www.epa.gov/etv.
DANETV was established by four independent Danish research and technology organizations and
is supported by the Danish Agency for Science, Technology and Innovation under the Danish
Ministry of Science, Technology and Innovation to provide environmental technology verification
for vendors of innovative water technologies. Information and DANETV documents are available at
www. etv-denmark. com.
DHI as DANETV Water Center performed the testing and verification in collaboration with ETV
Canada and the ETV Advanced Monitoring Systems (AMS) Center, managed by Battelle through a
cooperative agreement with the U.S. EPA.
VERIFICATION AND TEST DESCRIPTION
Testing and verification of the ECLOX Handheld Luminometer took place between January and
April 2010. DHI personnel, with support from the vendor, HACH-LANGE, coordinated and
supervised the testing and verification procedure. The testing took place in the DHI laboratories,
H0rsholm, Denmark. DHI operated the luminometer during the testing and verification procedure.
HACH-LANGE provided the product (luminometer, thermal block and software), user manuals,
and operating instructions for the tests. The Eclox verification test was performed simultaneously
with the verification test of LUMIStox 300 Bench Top Luminometer also from HACH-LANGE.
ETV Canada, Battelle and DHI jointly produced the verification protocol, test plan, process
document and report documents including this Verification Statement with input from HACH-
LANGE and an independent expert group.
The test program was designed to comply with ISO standard 11348-3: "Water quality -
Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri
(Luminescent bacteria test)" as well as the instrument manuals. However, the ECLOX with
LUMIStherm Thermal block and LUMISsoft4 Software does not fulfill all ISO 11348-3
requirements.
Stock solutions were prepared in 2% NaCl MilliQ water. Solid NaCl was added to wastewater to
obtain the salt concentration required for testing with the saltwater bacteria, Vibrio fischeri. Dilution
series were prepared with dilute saltwater (2% NaCl).
Ten test series in total were performed as outlined in the joint test plan, with deviations mentioned
in the report. Each test series provided information on specified performance parameters, as shown
in Table 1. One test series was performed on wastewater to provide performance information on
wastewater toxicity.
Page 2 of 6
-------
Table 1 Test design and associated performance parameters.
Performance parameters
Range, Repeatability, Agreement with accepted values
Criterion of detection
Robustness, effect of start concentration on repeatability
Reproducibility
Robustness, sample temperature at field use*
Robustness, sample temperature at laboratory use
Robustness, pH
Robustness, color
Robustness, turbidity
Robustness, matrix
Matrix
2% NaCI MilliQ
X
X
X
X
X
X
X
X
X
Waste water
X
* For this test the ECLOX was not connected to LUMIStherm Thermoblock and LUMISsoft4 Software. ECLOX firmware was used
instead of the software. This test was not in compliance with ISO 11348-3.
The operational conditions during testing are shown in Table 2.
Table 2 Operational parameters evaluated during testing.
Temperature of
thermoblock
14.0- 16.1 "C
pH in sample
6.0-8.5
Color correction
Colored samples
Turbid samples
Temperature at
field use
5 - 23°C
Wastewater types
Domestic
Industrial
Complete information on wastewater quality parameters is included in the verification report.
Quality Assurance
DHI provided internal review of documents and an audit of test performance. Battelle and ETV
Canada ensured that the verification and tests were planned and conducted to satisfy the
requirements of U.S. ETV and ETV Canada, including input and concurrence from their
stakeholder groups. Battelle, U.S. EPA, and ETV Canada quality assurance staff conducted a data
quality audit of at least 10% of the test data. Battelle conducted an on-site technical systems audit.
Three technical experts provided an independent review of the test plan, and four technical experts
reviewed the verification report. This statement reflects the verification results after performing the
above quality assurance actions.
TECHNOLOGY AND PRODUCT DESCRIPTION
The following description of the ECLOX Handheld Luminometer is
based on information provided by the vendor and does not represent
verified information.
The ECLOX is a portable instrument designed to provide data
appropriate for risk assessments in the event of environmental
releases, emergency situations, preventive security measures, and
regulatory monitoring.
The ECLOX is designed in particular to be used for the Luminescent
Bacteria Toxicity Test and to be used with a Chemiluminescence
Toxicity Test. Both tests will provide results in a short-term and
Page 3 of 6
-------
simple manner in the field or in the laboratory. The ECLOX used in the field gives values of
percent inhibition.
The ECLOX can be used to deliver luminescence values by connecting the thermoblock
LUMIStherm to a computer program (LUMISsoft4). This approach is used for testing and recording
data according to ISO 11348-3 for calculation of the Lowest Ineffective Dilution causing less than
20% inhibition (LID) and Effective Concentration causing 50% inhibition (ECso). In this
verification results have been obtained as ECso values or directly as percentage of inhibition.
VERIFICATION RESULTS
Table 3 Description of matrix and effect for ECLOX.
Matrix
Effect
ECLOX is applied for wastewater; river and lake
water; leachate from soils and waste; or directly in
effluent chemicals. Verification testing was
conducted on domestic and industrial wastewater
effluents.
Measurement of toxicity as indicated by inhibition
of luminescent bacteria by a variety of compounds
including metal ions, organic pesticides, inorganic
and organic pollutants, and surfactants.
Additional parameters included:
User manual quality, product cost, environmental
health and safety.
Performance Parameters
The performance parameters listed in Table 4 are results for ECso values or percent inhibition
generated after 30 minutes. Only 30-minute results are included here since they represent the
standard length of time for reporting ECso results.
Table 4 ECLOX performance parameter summary.
ECLOX
Compound
General
Zn2+
Cr2072-
Triclosan
Cyanide
SDS
CTAB
Criterion
of
detection
inhibition
5.5
Range of
application
mg/L
8.2-130
37 - 590
1.1 -17
35 - 570
2.0 - 32
1.9-31
Precision
Repeat-
ability
4.9
24
2.2
16
38
1.2
Reproduc-
ibility
51
Agreement
with accepted
values
186
96
190
99
Robustness
pH, color,
turbidity,
laboratory
temperature11
85-115
Field
temperature
(15 min)
11 -171
73-105
Waste-
water
matrix11
22-125
(-20) -13
101 -141
35-101
61 -73
1)For colored samples, robustness results are presented after the use of color correction. For the BaSO4-turbid samples, robustness
results are presented without the_use of color correction. For domestic wastewater, adjustment was made to account for the negative
inhibition from the wastewater; if color correction was used the robustness was 101% to 130%. The values listed in table are the best
achievable robustness.
Page 4 of 6
-------
General observations included the following:
• The user manual and other instructions were found to be complete.
• The results showed that the use of color correction is essential when testing colored samples,
while the results for turbid BaSC>4 samples showed that the color correction function is not
applicable. The applicability of color correction for other types of turbid samples was not tested.
• No additional risks for occupational health and environment were noted compared to
conventional effluent wastewater testing or analysis.
• The purchase price for the ECLOX, LUMIStherm, theLUMISoft4 software at the time of testing
was 6,500 Euro (9,600 $U.S.). Additional equipment such as cuvettes, bacteria and chemicals
on a cost-per-sample basis as used for testing for ECso according to the ISO 11348-3 was 18
Euro(23$U.S.).
• The product costs for one laboratory day are listed in Table 5. Shelf life of the dried bacteria is
one year, and the lifetime of the rehydrated bacteria is four hours, making it possible to perform
an ECso-test according to the ISO 11348-3 on three samples plus associated controls and
standards.
Table 5 List of capital cost items and operation and maintenance cost items per product unit.
Item type
Capital
Buildings and land
Equipment
Utility connections
Installation
Start up/training
Operation and maintenance
Materials, including chemicals
Utilities, including water and
energy
Labor
Number/duration and item
1 laboratory facility
1 ECLOX, 1 LUMIStherm, 1 PC with LUMISsoft4
3 power supplies
Can be done by operator/laboratory technician on
1 day
Training of laboratory technician, done in 1 day
1 bacterial batch, 20 cuvettes per sample (=60), 1 bottle of reconstitution
solution, 1 bottle of 2% NaCI solution, 1 bottle of solid NaCI
Power (PC and screen ~6 kWh, ECLOX 4 AA batteries)
One laboratory technician for 1 day
Original signed by Mette Tjener Andersson 27/4-11
Verification responsible
Head of DHIDANETV Water Center
Original signed by J0rn Rasmussen
Director, Group R&D and Quality Management, DHI
Original signed by Tracy Stenner
Manager, Environmental Solutions Product Line
Battelle
Original signed by Sally Gutierrez
Director, National Risk Management Research Laboratory
Office of Research and Development, U.S. EPA
Original signed by Mona El Hallak
Director Technology Assessment
And Quality Assurance Services
OCETA (ETV Canada)
Original signed by Kevin Jones
President and CEO
OCETA (ETV Canada)
Page 5 of 6
-------
NOTICE: ETV verifications are based on an evaluation of technology performance under specific,
predetermined operational conditions and parameters, and the appropriate quality assurance
procedures. DANETV, DHI, ETV Canada, U.S. EPA and Battelle make no expressed or implied
warranties as to the performance of the technology and do not certify that a technology will always
operate as verified. The end user is solely responsible for complying with any and all applicable
regulatory requirements. Mention of commercial product names does not imply endorsement.
Page 6 of 6
------- |