842-R-08-001
Ocean Survey Vessel Bold
Annual Report 2007

Monitoring and Assessing
the Health of Our Ocean and
Coastal Waters
               ••••inn

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 1  Executive Summary



 3  Introduction



 4  The OSV Bold



 6  Green Activities On-Board the OSV Bold



 8  2007 Survey Highlights



    9   Gulf of Mexico Hypoxia Assessment and Monitoring



    10  Maintaining Depth of Shipping Channels to U.S. Ports:



        Ocean Dredged Material Disposal Site Assessment and Monitoring



    11  Coral Reef Monitoring and Biocriteria Development



    12  Coastal Water, Sediment, and Organism Toxicity Assessments



    13  Fish Waste Disposal Site Assessment and Monitoring



    13  Coastal Eutrophication Assessment



    14  Ocean Outfall Monitoring



    14  Disposal Site Surveys



    15  Support to Federal, State, Territorial, and Academic Partners



    18  Public Education on EPA Oceans and Coastal Programs



    18  Surveys in 2008



19  Appendix 1:  OSV Bold Facilities and Technical Equipment



21  Appendix 2:  OSV Bold Scientific Surveys in 2007


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        The mission of EPA's Ocean Survey Vessel Bold (OSV Bold} is to monitor and assess the
        health of our oceans and coastal waters. The information gathered by the OSV Bold al-
        lows EPA to more effectively prevent effects from numerous pollution sources, whether
from land or from the ocean. Acting as a floating laboratory, the OSV Bold is helping to chart a
healthier course for our oceans, so they may be enjoyed for years to come.
   This is the second OSV Bold Annual Report. It highlights the ship's scientific survey capabili-
ties, accomplishments,  and the unique role  that this vessel plays  in supporting EPA's monitor-
ing and  assessment programs.  In 2007, the OSV Bold supported scientific surveys over a wide
variety of geographic areas and missions, including an assessment of Gulf of Mexico hypoxia,
monitoring of ocean  dredged material disposal sites, monitoring ocean  outfalls, and assess-
ment of critical coral reef  habitats. During this period, the OSV Bold completed a  total of 40
oceanographic surveys, involving hundreds of sampling locations, while spending over 270 days
at sea.
2007 Survey Highlights:
   • Gathered data on two Gulf of Mexico hypoxia surveys in April  and August.
   • Conducted monitoring for 12 ocean dredged material disposal sites and one fish waste site
     managed by EPA.
   • Surveyed coral reef environments in the Caribbean Sea and in Florida waters.
   • Assessed coastal development impacts  on fisheries in New England.
   • Monitored  shellfish waste  disposal impacts to the  marine environment off  the coast
     of Virginia.
   • Assessed coastal eutrophication in the Mid-Atlantic Bight from New York to North Carolina.
   • Monitored five ocean  discharge outfalls for the effects on the marine environment in the
     Caribbean Sea, Mid-Atlantic Bight, and  Florida waters.
   • Supported federal, state, territorial, and academic partners.
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   Various survey partners contributed to the OSV Bold's second year of successful operation,
including U.S. Army Corps of Engineers, U.S. Navy, U.S. Virgin Islands Department of Planning
and Natural  Resources, National Estuary Program, National Oceanic and Atmospheric Adminis-
tration, University of Puerto Rico, University of Connecticut, and Rutgers University.
   In addition to supporting a number of diverse scientific surveys, more than 15 public educa-
tion events were held on the OSV Bold in 2007. The OSV Bold hosted these events in New York,
Connecticut, Rhode Island, Maryland, Florida, the U.S. Virgin Islands, and Puerto Rico. In Puerto
Rico, participants included National  Estuary Program Directors, local newspapers and schools,
and international non-governmental  organizations.
The OSV Bold at dock in Baltimore, Maryland.
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         Our oceans and coasts are unique re-
         sources that support a wide diversity
         of life.  We depend on these complex
ecosystems  to provide us with places  to  live,
play, relax,  and  work.  Our  national economy
is linked  in a number of ways  to the produc-
tivity of our oceans and coasts. For instance,
in 2004, the ocean economy contributed more
than $138 billion to American prosperity,  and
supported more than two million jobs, accord-
ing  to  market data from the National  Ocean
Economics Program. Of no  less value are the
marine resources that are difficult to measure
economically, such  as the beauty of our oceans
and coasts, their cultural significance, and the
vital ecosystem functions they provide.
   Human actions adversely affect our oceans
and coastal waters. Polluted  sediments can sig-
nificantly alter aquatic  ecosystems. Wastewa-
ter discharged from shore or from vessels  that
violate permit requirements can be a threat to
public health and marine life. Exposure to toxic
chemical and pathogenic contamination nega-
tively affects the entire food web. If not man-
aged properly, ocean and coastal resources can
be damaged by habitat modification, dredging,
construction, and other human activities.
   The future health of our ocean and coastal
resources depends on our actions  today. To
restore  and safeguard these resources,  EPA
undertakes many efforts to  identify and con-
trol problems that threaten the health of our
oceans and coastal waters. Gathering informa-
tion and analyzing data to support manage-
ment  decisions  are essential parts of marine
resource protection. The Ocean Survey Vessel
Bold (OSV Bold) surveys oceans and coasts to
protect human health, to support economic and
recreational activities,  and  to influence actions
that safeguard healthy habitat for fish, plants,
and wildlife.
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        The OSV Bold was constructed by the
        Tacoma Boat Building Company of Ta-
        coma, Washington, and was first com-
missioned on October 16, 1989, as the United
States Naval Ship (USNS) Vigorous, a Tactical
Auxiliary General Ocean Survey (T-AGOS) class
vessel. The vessel was later renamed the USNS
Bold and served on many surveillance  missions
in the Pacific Ocean. The Navy decommissioned
the USNS Bold in 2004. EPA acquired the ex-
USNS Bold on March 31, 2004, to replace the
Peter W. Anderson, EPA's previous  ocean sur-
vey vessel. EPA began  scientific surveys with
the OSV Bold on August 8, 2005.
   The OSV Bold underwent dramatic changes
in  her transformation from a military surveil-
lance vessel to an ocean and coastal  monitor-
ing vessel. EPA improved the deck machinery

   Quick Facts	
and added wet and dry laboratories, including
a data acquisition laboratory where information
is transmitted from the sampling equipment to
computers. Sampling equipment includes, but
is not limited to, a side scan sonar that produc-
es digital acoustic images of the ocean floor and
a water profiler that  measures physical water
characteristics in real-time throughout the wa-
ter column. For a detailed list of scientific facili-
ties and technical equipment on the OSV Bold,
see Appendix 1.
  The OSV Bold is managed by EPA's Oceans
and Coastal Protection Division, in the Office of
Water.  The EPA Vessel Manager, Kennard Potts,
provides direction to  Seaward Services,  Inc.
(EPA's contractor) which  handles the operation
and maintenance of the vessel.
  The OSV Bold provides EPA and its partners
     Overall length: 224 feet
     Width: 43 feet
     Draft: 15 feet
     Water displacement: 2300 tons
     Operating speed:  11 knots
     Ship operating crew: 19
     Scientific berths: 20
                                     The OSV Bo/d at sea.
Monitoring and Assessing the Health of Our Oceans and Coastal Waters

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a platform to gather the scientific data needed
to assess the marine environment, and to make
informed decisions to protect these resources
and human  health. EPA's partners in 2007 in-
cluded the U.S. Army Corps of Engineers,  U.S.
Navy, U.S. Virgin Islands Department of Plan-
ning and Natural Resources, National Estuary
Marine Protection, Research, and Sanctuaries
Act. The surveys provide scientific information
and data to support EPA's mission to protect
and enhance ocean and coastal waters through
a variety of programs, including partnerships
and regulatory actions, as well as response
to emergencies.  Surveys are  conducted by
Program,  National Oceanic and Atmospheric
Administration, University of Puerto Rico, Uni-
versity of Connecticut, and Rutgers University.
By the end of 2007, the OSV Bold had sup-
ported surveys along the Atlantic Coast, in the
Gulf of Mexico, and in the Caribbean Sea.
   Scientific surveys conducted on-board the
OSV Bold  address  requirements  of  Federal
statutes such as the Clean Water Act and the
                      The OSV Bold at sea.
scientists from various EPA offices, including
Regional Offices, Headquarters, and the Of-
fice of Research and Development; States and
Territories;  academic institutions; and  other
partners. All surveys are conducted under the
leadership  of EPA-certified  Chief Scientists,
who must  complete  a  rigorous  certification
program before serving in that capacity.
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        The OSV Bold supports EPA's mission to
        protect the ocean and coastal environ-
        ment. Staff and crew are dedicated to
operating the vessel in the most environmental-
ly sustainable way possible. Best management
practices coupled with the latest technologies en-
sure that the ship is not degrading the resources
that we seek to protect.  New technologies and
best management practices are utilized to reduce
the impact of sewage, oil, and hull coatings that
are part of the normal operation of the vessel.

No Discharge Practices
   The discharge of untreated or partially-treat-
ed human waste from vessels can contribute to
high bacteria counts and subsequent increased
human  health  risks.  These  problems can  be
particularly harmful  in lakes, slow-moving riv-
ers, marinas, and other bodies of water with low
flushing rates. Blackwater (sewage) and graywa-
ter (wastewater from showers, sinks, laundries,
and  kitchens) are kept as long as possible in
holding tanks on the OSV Bold. The waste in the
holding tanks is then pumped to an onshore fa-
cility. If a holding tank fills on a voyage, a marine
sanitation device treats and disinfects the waste.
After treatment, waste (containing blackwater
and graywater) is disposed of only beyond three
nautical miles from the shoreline.
   In addition, all shipboard-generated garbage,
cooking oils, and greases are collected  and dis-
posed of at onshore facilities. Any liquid collect-
ed in the bilge is disposed of onshore in special
reception facilities. Strict rules apply to disposal
of all chemicals used in ship laboratories.

Lower Sulfur Dioxide Emissions
   Sulfur dioxide is an air pollutant  that ships
generate and that can travel  over long distanc-
es.  It contributes to respiratory illness and the
formation of acid rain. Whenever available, the
OSV Bold uses an  ultra low-sulfur fuel that sig-
nificantly cuts down on harmful air emissions.

A Better Hull Coating
   Hull  coatings prevent  corrosion  as well as
biological growth, reducing drag and increasing
fuel  efficiency of a vessel. The hull coating on
the OSV Bold does not contain organotin pes-
ticides,  and is  certified as compliant with the
International  Maritime Organization's  Interna-
tional Convention on the control of harmful anti-
foulants. Information collected by EPA and the
Department of Defense (DoD) indicates that the
hull coating used on the OSV Bold has the lowest
copper leach  rate of hull coatings approved for
use on DoD vessels. A lower leach rate means a
lower impact to surrounding waters.
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Non-toxic Firefighting Foam                  Ballast Water Management
   EPA uses a fire-fighting foam that can handle     Ballast water tanks temporarily  hold  water
any possible fires on the ship and  is also envi-  to provide draft (immersion depth of a vessel)
ronmentally safe. This protein-based foam  en-  and stability. Ballast water discharged and trans-
sures efficient fire control.                      ported by vessels may carry organisms from one
                                              waterbody to another. This is a main vector for
Best Management Practices                  introducing and spreading  aquatic invasive spe-
   The OSV Bold is operated with EPA's mission  cies (alien species that can cause harm to the
at the forefront. Best management practices are  environment and to human health). Aquatic in-
employed in daily operations of the  vessel. Oil is  vasive species have affected all coastal waters
carefully collected, and fueling of engines on  rig-  of the U.S., including Alaska,  Hawaii, and the
id-hull inflatable boats is done with the  utmost  Pacific Islands.
care to  avoid spills.  Special absorbent products     As part of standard operating procedure, bal-
and materials  are  used to collect  drips of oil,  last water exchange (emptying and refilling bal-
grease,  or fuel.                                 last tanks) is done at sea to limit transfer of in-
   In addition, EPA supports shipyards that em-  vasive species between ports by the OSV Bold.
ploy creativity and innovation to make their op-
erations more environmentally sustainable.
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     In 2007, the OSV Bold conducted scientific   tions, while spending over 270 days at sea. In
     surveys in the Atlantic Ocean, the Gulf of   addition, the OSV Bold held educational events
     Mexico, and the Caribbean Sea. The OSV   in New York, Connecticut, Rhode Island,  Mary-
Bold completed a total of 40 oceanographic   land, Florida, the U.S. Virgin Islands, and Puer-
surveys, involving hundreds of sampling loca-   to Rico.
Figure 1: Scope of OSV Bold Monitoring and Assessments in 2007.
                                                                          CT  ".Rhode Island Sound
                                                                             Eastern Long Island Sound
                                                                           Historic Area Remediation Site,
                                                                           New Jersey
                                                                         Bethany Beach, DE
                                                                         'Ocean City, MD
                                                                    ipe Canaveral, FL
                                                                    Brevard County, FL
                                                                     ,Palm Beach, FL
                                                                      Boynton/Delray Beach, FL
                                                                     Port Everglades, FL
                                                                     Miami, FL
                                                                   Florida Keys National Marine Sanctuary
                                      Areclbo   San Juan
                                                              St. Croix, USVI
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Gulf Of Mexico Hypoxia                 elusion of an Annual  Operating  Plan and An-
Assessment and Monitoring           nual  Report. These  documents  will  provide
   Since 1985, oxygen-depleted (or hypoxic)   mechanisms for  maintaining  and  tracking
waters  have  been measured  in the  Gulf of   progress  between  reassessments, state-led
Mexico, off the coast of Louisiana and Texas,   nutrient reduction strategies, complementary
The OSV Bold contributes to the assessment   federal strategies, and an outreach plan to en-
and monitoring of hypoxia in the Gulf  by per-   gage stakeholders.
forming surveys of the hypoxic zone.              The Action  Plan calls for expanded  long-
   Hypoxia occurs when  oxygen concentra-   term  monitoring  programs and  enhanced
tions are less than two milligrams per liter, lev-   research and  modeling efforts.  In 2007, the
els that can not sustain most marine life.  The   OSV  Bold supported  this  key action through
size of  the hypoxic zone  varies considerably   research efforts to characterize the magnitude
each year, depending on natural and human-   of, and variability in, physical,  chemical, and
influenced factors. In 2007, the measured size   biological properties and processes in the wa-
of the  hypoxic zone was 20,500 square kilo-   ter column.  These monitoring activities help to
meters (7,900 square miles), about the size of   better define the spatial and temporal  extent
the State of Massachusetts. This was the third   and dynamics of the hypoxic zone.
largest hypoxic zone since measurements be-     Seasonal  data and  information collected
gan in 1985.  Current evidence indicates that   include the  distribution and variability  in dis-
the development, extent, and persistence of   solved   inorganic  nutrient  concentrations;
hypoxia in the Gulf  of  Mexico are primarily   particulate nutrients;  total suspended  solids;
caused  by human-influenced factors including   phytoplankton species biomass and pigments;
nutrient loading  from the  Mississippi-Atchafa-   temperature; salinity; dissolved oxygen; and
laya River Basin.                              light attenuation (decreased light intensity with
   Monitoring  and assessment by  the OSV   increasing water depth). The data will further
Bold supports the Action Plan  for Reducing,   refine a predictive model for Gulf of Mexico hy-
Mitigating, and Controlling Hypoxia  in the Gulf   poxia, and should reduce scientific uncertainty
of Mexico. The 2001 Action Plan was released   about nitrogen and  phosphorus  loads, and the
by the Mississippi River/Gulf of Mexico Water-   formation, extent,  duration, and severity  of
shed Nutrient Task Force, a Federal and State   the hypoxic  zone. This information will support
agency  working  group. A revised Action Plan   and provide adaptive management options to
was released  in  June 2008, and will increase   reduce the five-year average size of the zone
accountability  and specificity through  the  in-   to less than 5,000 square kilometers  (about
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         1,900 square miles) as outlined in the Action
         Plan.  The five-year average for 2003 to 2007
         is  14,644 square  kilometers  (5,600  square
         miles), more than twice the size of the goal.

         Maintaining Depth of Shipping
         Channels to U.S. Ports: Ocean
         Dredged Material Disposal Site
         Assessment and Monitoring
         EPA scientists deploy a rocking chair
         dredge to collect oceanic organisms and
         sediments from the seafloor.
            The nation's  ports, harbors, and  navigable
         waterways  are vital to the U.S. economy and
         national security. Dredging, the removal of sedi-
         ments  to maintain access to those areas, has
         become increasingly important as ships increase
         in size. Dredged material disposal must be con-
         ducted in a  safe  and environmentally acceptable
         manner under the Marine Protection,  Research,
         and Sanctuaries Act (MPRSA), or the Clean Wa-
         ter Act (jurisdiction is determined by the location
                                            of the disposal site). An important mission of the
                                            OSV Bold is to collect environmental data that
                                            can be used to designate new disposal sites and
                                            to perform  the required periodic monitoring of
                                            existing designated ocean  disposal sites. Under
                                            the MPRSA, EPA is responsible for designating
                                            ocean disposal sites, and for reviewing and con-
                                            curring on dredged material ocean disposal per-
                                            mits issued  by the U.S. Army Corps of Engineers.
                                            The MPRSA also requires that a site monitoring
                                            and management plan be prepared for each site
                                            before it may be used for dredged material dis-
                                            posal. An essential component of site monitoring
                                            and management plans is a characterization of
                                            baseline conditions before any disposal  activity
                                            is conducted at the site, so that changes in ben-
                                            thic habitat  resulting from disposal activities can
                                            be identified during future surveys.
                                               EPA regional  scientists used the OSV Bold to
                                            assess and  monitor conditions at a number of
                                            ocean dredged material disposal sites (ODMDS).
                                            For example, the OSV Bold, in conjunction with
                                            the U.S. Army Corps of Engineers'Antilles Office
                                            and University of Puerto Rico (UPR), was used to
                                            sample sediment at the Arecibo Harbor ODMDS.
                                            Samples  of bottom sediment  from various lo-
                                            cations within and around the boundaries of
                                            the site were collected to determine the variabil-
                                            ity of baseline chemical and physical sediment
                                            characteristics.
                                               EPA regional  scientists also use the OSV
                                            Bold to confirm  that  dredged material is dis-
                                            posed of properly at ocean disposal sites, that
                                            the dumping does not unreasonably degrade
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Monitoring and Assessing the Health of Our Oceans and Coastal Waters

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or endanger human health or the  marine  en-
vironment, and that the site  is performing as
it was  designed.  For example, the OSV Bold
was used to measure dissolved organic tin  lev-
els in the water and sediment at two ODMDS in
Florida: Cape Canaveral and Miami. The surveys
confirmed that there are elevated levels  of or-
ganic tins (tributyl, dibutyl, and monobutyl  tin)
at these sites.  Future surveys will be designed
to assess the extent of the areas with elevated
organic tin levels and potential environmental
impacts from increased levels of tin.
   The OSV Bold also  helps in locating and as-
sessing potential new areas to receive dredged
material.  For example, sediment mapping sur-
veys conducted offshore of the  Chandeleur Is-
lands, Louisiana, determined the baseline condi-
tions for the proposed Gulfport Offshore ODMDS.
Dredging  maintains waterways on which  the
U.S. commerce and military depend.  Selection
of appropriate sites to receive suitable dredged
material and continued monitoring of these sites
helps to protect the marine environment.
   The following locations have  ocean dredged
material  disposal sites that were surveyed in
2007:
   • Arecibo Harbor, Puerto Rico
   • Cape Canaveral, Florida
   • Fort  Pierce, Florida
   • Miami, Florida
   • Palm Beach Harbor, Florida
   • Port  Everglades, Florida
   • Dam Neck, Virginia
   • Eastern Long Island Sound, Connecticut
Coral Reef Monitoring and
Biocriteria Development
   Coral reefs are declining  in many areas of
the world, partly because of increasing sea wa-
ter temperatures, as well as effects from local
EPA divers survey coral reef habitat.

sources,  such as excessive nutrient loading,
sedimentation, and  direct physical damage to
coral  populations.  Coral reefs are  important
ecosystems  because they provide habitat for
many fish and invertebrate species. The struc-
ture of a well-developed  and diverse  coral
community  supports  fisheries, tourism, and
research opportunities, and protects shorelines
from erosion by  waves and currents. In addi-
tion, corals are important sensitive sentinels of
water quality and general ecological health.
   A coral condition survey was  conducted in
St.  Croix, U.S.  Virgin  Islands (U.S.V.I.). The
primary purpose of this survey was to assist
the U.S.V.I,  government  in  developing  new
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         strategies and assessment tools to define bio-
         criteria for coral reefs.  Biocriteria are narra-
         tive descriptions or numeric values that repre-
         sent the biological condition of the community.
         Standards  based on biocriteria are  powerful
         management tools because biological commu-
         nities are dependable indicators of the health
         of an aquatic ecosystem. Strategic  observa-
         tion and documentation of the coral conditions
         were  performed using the Stony  Coral Rapid
         Bioassessment Protocol  developed  by  EPA's
         Office of Research and Development.

         Coastal Water, Sediment, and
         Organism Toxicity  Assessments
            The OSV Bold performed numerous surveys
         to assess contaminant concentrations in sedi-
         ments, water, and organisms.
                                               The OSV Bold supported a survey to assess
                                            the importance of near-shore habitats on winter
                                            flounder fisheries off the coast of Rhode Island.
                                            Winter flounder is a commercially and recreation-
                                            ally important fish species whose population has
                                            declined dramatically in the past 25 years. Near-
                                            shore habitats provide critical nursery areas for
                                            winter flounder that develop offshore  into the
                                            adult population. Previous studies conducted by
                                            EPA identified chemical fingerprints (i.e., stable
                                            isotopes and trace metals) of the otoliths (ear
                                            bones)  of juvenile flounder residing  in nursery
                                            areas within Narragansett Bay,  Narrow  River,
                                            and coastal salt ponds. After using the OSV Bold
                                            to collect adult offshore winter flounder, scien-
                                            tists removed the juvenile cores (i.e., calcium
                                            carbonate deposits  in otoliths)  to attempt to
                                            match them to chemical fingerprints  previously
         EPA scientists collect winter flounder, along with starfish and stingrays, aboard
         the OSV Bold.
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Monitoring and Assessing the Health of Our Oceans and Coastal Waters

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identified for nursery areas. Growth patterns in   shell, and blue mussel. Hard substrate commu-
otoliths help us to understand life cycles in fish   nities found on both small gravel and boulder-
populations, and through chemical fingerprint-   size rocks (some of which were adjacent to
ing of these structures, scientists hope to iden-   the  proposed pipeline route) supported  finger
tify what critical habitats support recruitment to   sponges, northern star coral, blue mussel,  and
the adult offshore winter flounder population.     erect bryozoans. The survey team demonstrated
   The  OSV Bold also supported a survey  to   that a combined approach, using acoustic (mul-
identify the natural  bottom features and sedi-   tibeam  sonar and side  scan  sonar)  and video
ment characteristics of significant  benthic habi-   imaging, was useful for characterizing benthic
tats in Long Island Sound. Data from this survey   habitats and communities.
will provide information on the influence of gas
pipelines on these sites. Collaborators  on this   Fish Waste Disposal Site
project included the EPA Long Island Sound Of-   Assessment and Monitoring
fice; Connecticut Department of Environmental     The  OSV Bold supported the annual  survey
Protection; and University of Connecticut's Na-   at a shellfish waste disposal site off the  Virgin-
tional Undersea  Research Center (NURC) and   ia Capes.  The surveys  evaluated the effect of
Department of Marine Sciences. They evaluated   this disposal on the marine environment.  The
approaches to identify critical  benthic habitats   survey  investigated whether the site was being
in Stratford Shoal, which is a  topographic rise   degraded by the placement of shellfish  waste.
in Long Island Sound where  a gas  pipeline is
proposed  for installation. Existing multibeam   Coastal  Eutrophication
sonar and side scan  sonar maps were  used  to   Assessment
select sites for the survey. Using the OSV Bold's     A coastal  eutrophication survey aboard  the
side scan  sonar, the  survey team took  images   OSV Bold  continued a   previously established
of the sediment and determined the spatial dis-   coastal  trend  monitoring plan effort that is ex-
tribution of lobster-attributed "burrow features."   amining eutrophication in the Mid-Atlantic Bight.
The side scan sonar data were collected in con-   Data from the survey is being used to determine
junction with benthic grab samples, as  well  as   if coastal eutrophication, an increase in chemical
video footage from the NURC underwater video   nutrients such as nitrogen  and  phosphorus, off
camera array. The survey team also observed   the coasts of Virginia, Maryland, and Delaware is
general characteristics of benthic habitats and   improving  or declining, and what management
organisms across the varying sediments. Areas   actions  should be considered to improve water
with coarser sediments  were characterized by   quality.
dead mollusk shells such as razor clam, slipper

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         Ocean Outfall Monitoring             the survey will help scientists monitor condi-
            The OSV Bold supported three surveys on  tions of the VIRIL discharge to determine the
         the  impacts  of ocean outfall discharges on  effectiveness of current mitigation practices.
         coastal waters in 2007.
            Off the  coast of Palm  Beach, Florida, the  Disposal Site Surveys
         OSV Bold conducted  a survey to assess the     The Historic Area Remediation Site (HARS),
         potential effect of discharges from the Delray  a site off the coast of New Jersey which was
         Outfall on Gulf Stream reef health. The  OSV  historically used for disposal  of contaminated
         Bold sampled five coral colonies off Horseshoe  dredged material, is being remediated with un-
         Reef in  Palm Beach.   Stress responses of the  contaminated dredged material. A survey, sup-
         coral  to  outfall effluent were measured  and  ported by the OSV Bold, assessed the condition
         monitored. The data were  used  to determine  of the remediated surfaces, including changes
         the relationship between  the outfall  and in-  to the benthic habitat and movement of mate-
         creased harmful cyanobacteria blooms occur-  rials at and around the site. The OSV Bold used
         ring offshore of Palm Beach.                  side scan  sonar and sediment characterization
            Another study using the OSV Bold inves-  data to determine conditions at the site. These
         tigated  waters  in the Mid-Atlantic Bight near  data will be used to identify specific sediment
         three ocean  outfalls  in Delaware, Maryland,  types and to  develop  a benthic map of sedi-
         and Virginia. Levels of bacterial contamination  ment types at the HARS.
         due to Enterococci were  assessed.  The  pur-     In December 2004, the U.S. Coast Guard
         pose  of these efforts was to monitor the ef-  sunk the  60-foot steel-hulled Fishing  Vessel
         feet of the disposal of pollutants in accordance  Miss Dottie (F/V Miss Dottie} because the ship
         with the requirements of section 403(c) of the  was on fire, and drifting towards  the  Florida
         Clean Water Act.                             Keys National  Marine  Sanctuary.  The Coast
            Another ocean outfall  study took place  in  Guard sunk  the  vessel to  minimize damage
         the Caribbean Sea. In a 2003 survey,  the Vir-  to the Sanctuary.  However, after  sinking the
         gin Islands Rum Industries, Ltd. (VIRIL) ocean  vessel, the Coast Guard  determined that the
         outfall discharge  was assessed  and the site  disposal may  have occurred within the  Florida
         was biologically characterized. The survey re-  Keys National Marine Sanctuary's boundaries.
         suits showed potential for aquatic toxicity and  In October 2007, a survey was conducted on
         detrimental  light  attenuation. In 2007,  field  the OSV  Bold to locate the  F/V Miss  Dottie,
         observations, including video footage of the  since the Coast Guard only  provided  an ap-
         outfall discharge and surrounding area, were  proximate location. Side scan sonar images in-
         obtained from the site. The data obtained from  dicated that the vessel was intact and located

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within the Florida  Keys  Particularly Sensitive  the Loiza River (approximately 0.25 to 1 mile
Sea Area's boundaries. Bottom features in the  offshore). This area is composed of cemented
vicinity of the vessel also were observed using  sand dunes with coral and other benthic corn-
side scan sonar. Considering the vessel's loca-  munities. The offshore habitats support a di-
tion and proximity to the Sanctuary, scientists  verse collection of marine and estuarine fish
will assess potential impacts on the marine en-  and invertebrates, some of which are impor-
vironment in the future.                       tant for recreational and commercial fishing. A
                                            side scan sonar survey was performed to pro-
Support to Federal, State,             vide comprehensive benthic imaging of the off-
Territorial, and Academic             shore habitats. The side scan sonar data were
Partners                                  collected in  conjunction  with  video  footage
   From late October to early December 2007,  shot from a remotely-operated vehicle. The
the OSV Bold supported surveys to monitor  data will be used to classify the bottom types
and assess coastal conditions in EPA's Carib-  identified by side scan sonar, and to determine
bean jurisdictions—Puerto  Rico  and U.S. Vir-  the percent cover for  each bottom type found.
gin Islands (U.S.V.I.). A number of partners  In  addition,  conductivity-temperature-depth
contributed to the success of the  Caribbean  measurements were taken beyond the seaward
Initiative 2007 surveys,  including U.S.V.I.  De-  entrance of the San Juan Bay Channel to de-
partment of Planning and  Natural Resources,  scribe river plume behavior (the way in which
University of Puerto Rico,  Rutgers  University,  mixtures of river water, nutrients, sediments,
U.S.  Army Corps of  Engineers, U.S. Depart-  and marine organisms flow into the ocean).
ment of Energy, and National  Oceanic and At-     Sponsored by EPA and U.S.  Department of
mospheric Administration  (NOAA).  In Puerto  Energy,  research groups from UPR and  Rut-
Rico and U.S.V.I., several surveys were under-  gers University Coastal Ocean Observing Lab-
taken to support various  EPA programs, as well  oratory used the OSV Bold to study waters of
as cooperative efforts with federal, common-  the Nona  Passage, separating the islands  of
wealth, and academic partners.                Puerto Rico and Hispaniola.   The Nona  Pas-
   In collaboration with the San Juan Bay Es-  sage is  influenced by complex hydrodynamic
tuary (SJBE) Program and University of Puerto  processes. These processes are believed to in-
Rico (UPR), the OSV Bold conducted a benthic  fluence microbial ecology and light attenuation
habitat and  water  quality assessment of San  in the water column. The research groups also
Juan Bay and the  offshore environment. The  monitored water quality indicators in the Nona
offshore area consists of areas along the SJBE  Passage using  an autonomous  underwater
shoreline, from the mouth  of San Juan Bay to  vehicle.

                                       EPA's Ocean Survey Vessel Bold 2007 Annual Report        15

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         EPA Chief Scientists on the bow of the OSV Bold.
         In Rhode Island Sound, EPA scientists study the aquatic organisms collected during the
         winter flounder survey.
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While at dock in Newport, Rhode Island, the OSV Bold is open to visitors for a public
education event.
The OSV Bold at dock in Long Island, NY.
                                     EPA's Ocean Survey Vessel Bold 2007 Annual Report
17

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            Using the OSV Bold, EPA and NOAA conduct-
         ed a side scan sonar survey to provide compre-
         hensive benthic and shelf reef habitat imaging of
         Lang Bank in St. Croix, U.S.V.I. The data will be
         used to characterize the structure of, and quan-
         tify the extent of, near-shore habitats that sup-
         port important fish species off Lang  Bank.  In
         addition to the survey, NOAA scientists were in-
         vited aboard for collaboration and cross-training
         in  protocols  for coral reef monitoring, such as
         EPA's Stony Coral Rapid Bioassessment Protocol,
         and NOAA's protocols for fish counting and ben-
         thic biological and structural characterization.

         Public Education on EPA Oceans
         and Coastal Programs
         When in  port between scientific surveys, the
         OSV Bold was used for environmental education
         on challenging issues facing the health of marine
         waters. The OSV Bold hosted open visits for the
         public in New York, Connecticut, Rhode Island,
         Maryland, Florida, the U.S.V.I., and Puerto Rico.
                                            In Puerto  Rico, the  OSV Bold held two public
                                            outreach events in November, coordinating with
                                            the San Juan Bay Estuary Program and the Fall
                                            meeting of the Association of National  Estuary
                                            Programs. Participants included National  Estu-
                                            ary Program Directors, local newspapers and
                                            schools, and international  non-governmental
                                            organizations.

                                            Surveys in  2008
                                               In  2008, the OSV Bold is supporting sur-
                                            veys on the West Coast of the United States.
                                            Surveys occurring in 2008 include:
                                               • Maintaining Depth of Shipping Channels to
                                                U.S. Ports:  Ocean Dredged  Material Dis-
                                                posal Site Assessment and Monitoring
                                               • Coastal Water,  Sediment, and Organism
                                                Toxicity Assessments
                                               • Alaska Cruise Ship Survey
                                               • Puget Sound Survey
                                               • Ocean Outfall Monitoring
                                               • Disposal Site Surveys
         The OSV Bold at dock in Baltimore,
         Maryland.
                                            The OSV Bold in the Panama Canal, heading
                                            for the West Coast.
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OSV Bold Facilities and Technical
Equipment
Scientific Facilities
   • Wet Laboratory:  Equipped with sieve station
    (i.e., sieving table and trays), wash station
    with hot and cold freshwater and saltwater,
    ice machine (for sample  preservation), re-
    frigerator, electronic  navigation data ports,
    and electronic navigation  chart display with
    ship's location  and navigation information.
   • Survey  Operations Center:  Equipped with
  refrigerators,  freezers,  sub-zero  freezers,
  distilled water, computers, storage space,
  microscopes, and about 85 linear feet of lab
  benches.
• Microbiology  Laboratory: Equipped with au-
  toclave and incubator.
• Data Acquisition  Center:  Equipped  with
  computer systems to support digital data
  recorded from side scan sonar operations;
  water profiler deployment; and underwater
  video filming.
A new A-frame installed on the stern of the OSV Bold.
                                        EPA's Ocean Survey Vessel Bold 2007 Annual Report
                                                19

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         In Eastern Long Island Sound, technicians
         prepare a Klein 3000 side scan sonar for
         surveying an ocean dredged material
         disposal site.
         Sampling Equipment
            • Klein 3000 Side Scan Sonar: Produces digi-
              tal acoustic images of ocean floor.
            • Conductivity, Temperature, and Depth Wa-
              ter Profiler:  Measures physical water char-
              acteristics in situ in real-time throughout
              the water column.
            • Rosette Water Sampler: Collects water at
              specified depths in the water column.
                                               • Sediment Sampling Equipment: A variety of
                                                grabs and corers are available for the col-
                                                lection of sediments from the ocean floor.
                                               • Dredges: Collect oceanic organisms from
                                                the seafloor and sediments.
                                               • Sampling Nets: Collect oceanic organisms,
                                                such  as  fish and plankton,  from various
                                                depths in the water column.
                                               • A-Frame: Assists the deployment and re-
                                                trieval of the side scan sonar and  sedi-
                                                ment sampling equipment.

                                               Diver Operation Capabilities
                                               • Rigid-Hulled Inflatable Boat (RHIB):  At all
                                                times, the OSV Bold carries two RHIBs to
                                                support dive operations.
                                               • Dive  Locker:  Nitrox/Air  compressor, 31
                                                SCUBA tanks,  diver communication de-
                                                vices (i.e.,  diver-to-diver,  diver-to-sur-
                                                face), diver recall  system for emergency
                                                situations, dry  suits, and full-face masks
                                                available for use by certified diving  per-
                                                sonnel.
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OSV Bold Scientific Surveys in 2007
Survey
Location Month(s)
Gulf of Mexico Hypoxia Assessment and
Monitoring
Maintaining Depth of
Ports: Ocean Dredgei
Assessment and Mon
Shipping Channels to U.S.
1 Material Disposal Site
toring
Ocean Dredged Material Disposal Reference Site
Survey
Coral Reef Monitorinc
Development
Coastal Water,
Sediment, and
Organism Toxicity
Assessments
and Reef Biocriteria
Coastal Fish Tissue
Contaminant Assessment
Fish Waste Disposal Site
Monitoring
Coastal Eutrophication Assessment
Ocean Outfall Monitoring

Disposal Site
Surveys
Support to Federal,
State, Territorial,
and Academic
Partners

Vessel Disposal Site
Assessment
Historical Area
Remediation Site Survey
Hydrodynamic Processes
in the Mona Passage
Structural
Characterization of
Lang Bank
San Juan Bay National
Estuary Program
Gulf of Mexico
Cape Canaveral, Florida
Fort Pierce, Florida
Eastern Long Island Sound,
Connecticut
Dam Neck, Virginia
Miami, Florida
Palm Beach Harbor, Florida
Port Everglades, Florida
Arecibo, Puerto Rico
Brevard County, Florida
Broward and Dade Counties, Florida
Palm Beach County, Florida
Rhode Island Sound
Southern Virginia
Mid-Atlantic Bight
Delray Outfall, Florida
Mid-Atlantic Bight
Virgin Islands Rum Industries
Outfall, U.S. Virgin Islands
Florida Keys
Historic Area Remediation Site, New
Jersey
Mona Passage, Puerto Rico
St. Croix, US Virgin Islands
San Juan, Puerto Rico
April and August
March
March
July
August
October
October
October
November
March
October
March and September
July
August
July-August
March and September
August
November- December
October
June
October-November
November
November
                               EPA's Ocean Survey Vessel Bold 2007 Annual Report
21

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Ocean Survey Vessel Bold
Annual Report 2007

Monitoring and Assessing
the Health of Our Ocean and
Coastal Waters

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