United States
      Environmental Protection
      Agency
                                                             EPA-SAB-11-013 | August 2011 | www.epa.gov/sab
*SfBE?
            ence Advisory Board
            ce of the Administrator

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Cover photos by Eric Vance and other photographers, U.S. EPA. courtesy of U.S. EPA.

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               UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                                     WASHINGTON, DC 20460


                                            August 18, 2011


EPA-SAB-11-013                                                         OFFICE OF THE ADMINISTRATOR
                                                                           SCIENCE ADVISORY BOARD

The Honorable Lisa P. Jackson
Administrator
U.S. Environmental Protection Agency
1200 Pennsylvania Avenue, N. W
Washington, D.C. 20460

   Subject:  Reactive Nitrogen in the United States: An Analysis of Inputs, Flows, Consequences, and Management
           Options - A Report of the EPA Science Advisory Board

Dear Administrator Jackson:

   Excess reactive nitrogen compounds in the environment are associated with many large-scale environmental concerns,
including eutrophication of surface waters, toxic algae blooms, hypoxia, acid rain, nitrogen saturation in forests, and
global warming. In addition, reactive nitrogen is associated with harmful human health effects caused by air pollution
and drinking water contamination. Reactive nitrogen (hereafter referred to as Nr) includes all biologically active,
chemically reactive, and radiatively active nitrogen compounds in the atmosphere and biosphere of the earth, in contrast
to non-reactive gaseous N2. EPA and other federal and state agencies have implemented programs to reduce the risks
posed by excessive Nr, but a more comprehensive and integrated approach is needed to manage the use of Nr in a way
to achieve its benefits, such as fertilizer for food production, and mitigate its damages as it is introduced to and cycles
repeatedly through the environment in different forms and media.
   The Science Advisory Board (SAB) Integrated Nitrogen Committee has conducted a study to analyze sources and
fate of Nr in the United States and provide advice to EPA on integrated nitrogen research and control strategies. We are
pleased to submit the SAB report, Reactive Nitrogen in the United States: An Analysis of Inputs, Flows, Consequences,
and Management Options. Our objectives for this study were to:
   • Identify and analyze from a scientific perspective the problems Nr presents in the environment and the links among
    them;
   • Evaluate the contribution an integrated nitrogen management  strategy could make to environmental protection;
   • Identify additional risk management options for EPA's consideration; and
   • Make recommendations to EPA concerning improvements in nitrogen research to support risk reduction.
The SAB report provides findings and recommendations addressing these study objectives. Assessment of the challenges
and costs to the Agency of implementing the recommendations is beyond the scope of the report.
   In general, the SAB finds that:
   • In the United States, human activities across multiple sources  currently introduce more than five times the Nr into
    the environment than natural processes. The largest U.S. sources of new Nr entering the U.S. environment include:
    the creation and use of synthetic fertilizers, Nr created by legumes, and the combustion of fossil fuels.
   • Much of the Nr used to ensure a plentiful supply of food, fiber and biofuel is released to the environment, as is the
    Nr formed during fossil fuel combustion.
   • The introduction of human created Nr into the environment degrades air and water quality, which can cause harmful
    algae blooms, hypoxia, fish kills, loss of drinking water potability, loss  of biodiversity, forest declines, and human
    health problems resulting in losses of billions of dollars  per year.

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   • Multiple strategies and actions exist to more effectively minimize the inputs of Nr to the environment and maximize
    nitrogen use efficiency.
The SAB provides the following overarching recommendations to improve the management of Nr.
   • The framing of the movement of nitrogen among various environmental reservoirs in terms of the nitrogen
    cascade concept provides a means for tracking nitrogen as it changes form and passes through multiple ecosystems
    and media. Given this complexity, innovative management systems and regulatory structures reflecting these
    characteristics of Nr are required to address the significant environmental and human health damage caused by Nr.
    New institutional structures and relationships that also reflect the multi-media and multi-form character of Nr and its
    flows and transformations through the environment will have to be created for effective control and management.
   • The SAB recommends an integrated approach to the management of Nr. This approach must use a combination
    of implementation mechanisms appropriate to the specific environmental and policy contexts and supported by
    critical research on the specific risks of Nr and on decreasing the risks of Nr. The approach must reflect an integrated
    policy that recognizes the complexity and trade-offs associated with the nitrogen cascade while recognizing that
    intervention points vary in terms of efficiency and cost effectiveness.
   • EPA should form an intra-Agency Nr management task force that will build on the existing breadth of Nr research
    and management capabilities within the Agency. Its objective should be to increase scientific understanding of:
    (1) Nr impacts on terrestrial and aquatic ecosystems, human health, and climate; (2) Nr-relevant monitoring
    requirements; and (3) the most efficient and cost effective means by which to decrease various adverse impacts  of Nr
    loads as they cascade through the environment.
   • Successful Nr management will require changes in the way EPA interacts with other agencies. The SAB
    recommends that EPA convene a reactive nitrogen inter-agency management task force with broad representation
    from other agencies and departments involved with Nr control or utilization. This is essential to coordinate
    federal programs that address Nr concerns and would help ensure clear responsibilities for monitoring, modeling,
    researching, and managing Nr in the environment. Similar efforts at coordination and joint action need to be made
    among and between agencies at both the state and federal level.
   In the context of addressing the specific study objectives, the SAB explored how an estimated 25 percent reduction
in Nr introduced into the environment might be achieved with existing technology in the coming 10 to 20 years through
actions that could be taken by EPA, other management authorities, and other public and private organizations. Specific
actions include increased controls of oxides of nitrogen, improved reactive nitrogen uptake by agricultural crops, large-
scale creation and restoration of wetlands for nitrogen removal in agricultural landscapes with high Nr in surface waters,
decreased loss  of reactive nitrogen from agricultural lands and animal feeding operations, and decreased discharge of
reactive nitrogen from point sources and developed  (urban) lands. However, dealing effectively with reactive nitrogen's
cascade through air, water and land will require an integrated management approach that is multi-media and multi-
stressor as suggested by recent initiatives by EPA's Office of Research and Development.
   The most important task for EPA and allied agencies and departments will be to effectively inform the public of the
costs and dangers of excess Nr. Without strong public support, the widespread efforts necessary to control Nr will not be
possible.
   In closing, we appreciate the opportunity to provide advice on this very important topic, and we look forward to
receiving your response.  The SAB stands ready to provide more information as it may be useful and would be pleased to
assist EPA in the implementation of the report's recommendations, if the EPA would find such support valuable.
                                                  Sincerely,


    /Signed/                               /Signed:/                                  /Signed/
 Dr. Otto C. Doering III                   Dr. James N. Galloway                   Dr. Thomas L. Theis
 Chair                                  Chair (2007-2009)                       Vice-Chair (2008-2009)
 Integrated Nitrogen                      Integrated Nitrogen Committee            Integrated Nitrogen Committee
 Committee                             Science Advisory Board                  Science Advisory Board
 Science Advisory Board

                                             /Signed/
                                        Dr. Deborah L. Swackhamer
                                        Chair
                                        Science Advisory Board

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U.S.  Environmental  Protection  Agency

Science Advisory  Board

Integrated Nitrogen Committee


CHAIR
Dr. Otto C. Doering III, Professor, Department of Agricultural Economics, Purdue University, W. Lafayette, IN

CHAIR (2007-2009)
Dr. James N. Galloway, SidmanP. Poole Professor of Environmental Sciences, Associate Dean for the Sciences,
College and Graduate School of Arts and Sciences, University of Virginia, Charlottesville, VA

VICE-CHAIR (2008-2009)
Dr. Thomas L. Theis, Director, Institute for Environmental Science and Policy, University of Illinois at Chicago,
Chicago, IL

MEMBERS
Dr. Viney Aneja, Professor, Department of Marine, Earth, and Atmospheric Sciences, School of Physical and
Mathematical Sciences, North Carolina State University, Raleigh, NC

Dr. Elizabeth Boyer, Associate Professor, School of Forest Resources and Assistant Director, Pennsylvania State
Institutes of Energy & the Environment, and Director, Pennsylvania Water Resources Research Center, Pennsylvania
State University, University Park, PA

Dr. Kenneth G. Cassman, Professor, Department of Agronomy and Horticulture, Institute of Agriculture and Natural
Resources, University of Nebraska, Lincoln, NE

Dr. Ellis B. Cowling, University Distinguished Professor At-Large Emeritus, Colleges of Natural Resources and
Agriculture and Life Sciences, North Carolina State University, Raleigh, NC

Dr. Russell R. Dickerson, Professor, Department of Atmospheric and Oceanic Science, The University of Maryland,
College Park, MD

Mr. William Herz, Vice President for Scientific Programs, The Fertilizer Institute, Washington, DC

Dr. Donald L. Hey, Executive Director, Wetlands Research, Inc, Chicago, IL

Dr. Richard Kohn, Professor, Animal Sciences Department, University of Maryland, College Park, MD

Dr. JoAnn S. Lighty, Chair and Professor, Chemical Engineering, University of Utah, Salt Lake City, UT

Dr. William Mitsch, Professor, Olentangy River Wetland Research Park, The Ohio State University, Columbus, OH

Dr. William Moomaw, Professor of International Environmental Policy and Director of the Center for International
Environment and Resource Policy, The Fletcher School of Law and Diplomacy, Tufts University, Medford, MA

Dr. Arvin Mosier, Visiting Professor, Agricultural and Biological Engineering Department, University of Florida, Mount
Pleasant, SC

Dr. Hans Paerl, Professor of Marine and Environmental Sciences, Institute of Marine Sciences, University of North
Carolina - Chapel Hill, Morehead City, NC

Dr. Bryan Shaw, Commissioner, Texas Commission on Environmental Quality, Austin, TX

Mr. Paul Stacey, Director, Bureau of Water Management and Land Reuse, Planning and Standards Division,
Connecticut Department of Environmental Protection, Hartford, CT

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Dr. Thomas Armitage, Designated Federal Officer (2009-present), U.S. Environmental Protection Agency,
Washington, DC

Dr. Angela Nugent, Designated Federal Officer (2009), U.S. Environmental Protection Agency, Washington, DC

Ms. Kathleen White, Designated Federal Officer (2007-2008), U.S. Environmental Protection Agency, Washington, DC

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U.S.  Environmental  Protection Agency
Science Advisory  Board

CHAIR
Dr. Deborah L. Swackhamer, Professor and Charles M. Denny, Jr., Chair in Science, Technology and Public Policy and
Co-Director of the Water Resources Center, Hubert H. Humphrey Institute of Public Affairs, University of Minnesota, St.
Paul, MN
SAB MEMBERS
Dr. David T. Allen, Professor, Department of Chemical Engineering, University of Texas, Austin, TX
Dr. Claudia Benitez-Nelson, Full Professor and Director of the Marine Science Program, Department of Earth and
Ocean Sciences, University of South Carolina, Columbia, SC
Dr. Timothy Buckley, Associate Professor and Chair, Division of Environmental Health Sciences, College of Public
Health, The Ohio State University, Columbus, OH
Dr. Patricia Buffler, Professor of Epidemiology and Dean Emerita, Department of Epidemiology, School of Public
Health, University of California, Berkeley, CA
Dr. Ingrid Burke, Director, Haub School and Ruckelshaus Institute of Environment and Natural Resources, University
of Wyoming, Laramie, WY
Dr. Thomas Burke, Professor, Department of Health Policy and Management, Johns Hopkins Bloomberg School of
Public Health, Johns Hopkins University, Baltimore, MD
Dr. Terry Daniel, Professor of Psychology and Natural Resources, Department of Psychology, School of Natural
Resources, University of Arizona, Tucson, AZ
Dr. George Daston, Victor Mills Society Research Fellow, Product Safety and Regulatory Affairs, Procter & Gamble,
Cincinnati, OH
Dr. Costel Denson, Managing Member, Costech Technologies, LLC, Newark, DE
Dr. Otto C. Doering III, Professor, Department of Agricultural Economics, Purdue University, W Lafayette, IN
Dr. David A. Dzombak, Walter J. Blenko Sr. Professor of Environmental Engineering, Department of Civil and
Environmental Engineering, College of Engineering, Carnegie Mellon University, Pittsburgh, PA
Dr. T. Taylor Eighmy, Vice President for Research, Office of the Vice President for Research, Texas Tech University,
Lubbock, TX
Dr. Elaine Faustman, Professor and Director, Institute for Risk Analysis and Risk Communication, School of Public
Health, University of Washington, Seattle, WA
Dr. John P. Giesy, Professor and Canada Research Chair, Veterinary Biomedical Sciences and Toxicology Centre,
University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Dr. Jeffrey K. Griffiths, Professor, Department of Public Health and Community Medicine, School of Medicine, Tufts
University, Boston, MA
Dr. James K. Hammitt, Professor, Center for Risk Analysis, Harvard University, Boston, MA
Dr. Bernd Kali n Professor Emeritus and Associate Director, Environmental Radiation Center, Georgia Institute of
Technology, Atlanta, GA
Dr. Agnes Kane, Professor and Chair, Department of Pathology and Laboratory Medicine, Brown University,
Providence, RI
Dr. Madhu Khanna, Professor, Department of Agricultural and Consumer Economics, University of Illinois at
Urbana-Champaign, Urbana, IL
Dr. Nancy K. Kim, Senior Executive, Health Research, Inc., Troy, NY

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Dr. Catherine Kling, Professor, Department of Economics, Iowa State University, Ames, IA
Dr. Kai Lee, Program Officer, Conservation and Science Program, David & Lucile Packard Foundation, Los Altos, CA
Dr. Cecil Lue-Hing, President, Cecil Lue-Hing & Associates Inc., Burr Pudge, IL
Dr. Floyd Malveaux, Executive Director, Merck Childhood Asthma Network, Inc., Washington, DC
Dr. Lee D. McMullen, Water Resources Practice Leader, Snyder & Associates, Inc., Ankeny, IA
Dr. Judith L. Meyer, Professor Emeritus, Odum School of Ecology, University of Georgia, Lopez Island, WA
Dr. James R. Mihelcic, Professor, Civil and Environmental Engineering, State of Florida 21st Century World Class
Scholar, University of South Florida, Tampa, FL
Dr. Jana Milford, Professor, Department of Mechanical Engineering, University of Colorado, Boulder, CO
Dr. Christine Moe, Eugene J. Gangarosa Professor, Hubert Department of Global Health, Rollins School of Public
Health, Emory University, Atlanta, GA
Dr. Horace Moo-Young, Dean and Professor, College of Engineering, Computer  Science, and Technology, California
State University, Los Angeles, CA
Dr. Eileen Murphy, Grants Facilitator, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ
Dr. Duncan Patten, Research Professor, Hydroecology Research Program, Department of Land Resources and
Environmental Sciences, Montana State University, Bozeman, MT
Dr. Stephen Polasky, Fesler-Lampert Professor of Ecological/Environmental Economics, Department of Applied
Economics, University of Minnesota, St. Paul, MN
Dr. C. Arden Pope III, Professor, Department of Economics, Brigham Young University, Provo, UT
Dr. Stephen M. Roberts, Professor, Department of Physiological Sciences, Director, Center for Environmental and
Human Toxicology, University of Florida, Gainesville, FL
Dr. Amanda Rodewald, Professor of Wildlife Ecology, School of Environment and Natural Resources, The Ohio State
University, Columbus, OH
Dr. Jonathan M. Samet, Professor and Flora L. Thornton Chair, Department of Preventive Medicine, University of
Southern California, Los Angeles, CA
Dr. James Sanders, Director and Professor, Skidaway Institute of Oceanography, Savannah, GA
Dr. Jerald Schnoor, Allen S. Henry Chair Professor, Department of Civil and Environmental Engineering, Co-Director,
Center for Global and Regional Environmental Research, University of Iowa, Iowa City, IA
Dr. Kathleen Segerson, Philip E. Austin Professor of Economics, Department of Economics, University of Connecticut,
Storrs, CT
Dr. Herman Taylor, Director, Principal Investigator, Jackson Heart Study,  University of Mississippi Medical Center,
Jackson, MS
Dr. Barton H. (Buzz) Thompson, Jr., Robert E. Paradise Professor of Natural Resources Law at the Stanford Law
School and Perry L. McCarty Director, Woods Institute for the Environment, Stanford University, Stanford, CA
Dr. Paige Tolbert, Professor and Chair, Department of Environmental Health, Rollins School of Public  Health, Emory
University, Atlanta, GA
Dr. John Vena, Professor and Department Head, Department of Epidemiology and Biostatistics, College of Public
Health, University of Georgia, Athens, GA
Dr. Thomas S. Wallsten, Professor and Chair, Department of Psychology, University of Maryland, College Park, MD
Dr. Robert Watts, Professor of Mechanical Engineering Emeritus, Tulane University, Annapolis, MD
Dr. R. Thomas Zoeller, Professor, Department of Biology, University of Massachusetts, Amherst, MA

Dr. Angela Nugent, Designated Federal Officer, U.S. Environmental Protection Agency, Washington, DC

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Acknowledgements
   The EPA Science Advisory Board Integrated Nitrogen Committee would like to acknowledge many individuals who
provided their perspectives and insights for the Committee's consideration in the development of this report.
   Invited speakers provided perspectives at public meetings of the Committee. These speakers included: the following
individuals from the U.S. EPA - Mr. Robert Bastian, Mr. Gilbert Castellanos, Mr. John Davies, Dr. Robin Dennis, Dr.
Jonathan Garber, Dr. Richard Haeuber, Dr. Alan Hecht, Mr. Rudolph Kapichak, Mr. Gary Lear, Dr. Richard Linthurst,
Dr. Rohit Mathur, Ms. Roberta Parry, and Mr. James Pendergast; the  following individuals from the USDA - Dr. Roger
Claassen, Dr. Stan Daberkow, Dr. Raymond Knighton, and Dr. Mark Walbridge; and the following individuals from other
organizations - Mr. Craig Cox, Soil and Water Conservation Society; Dr. Jan Willem Erisman, Energy Research Centre
of the Netherlands; Dr. Paul Fixen, International Plant Nutrition Institute; Dr. David McNaught, Environmental Defense
Fund; Dr. John Miranowski, Iowa State University; Dr. Martin Petrovic, Cornell University; and Mr. John Sheehan,
LiveFuels.
   The Committee would also like to thank the speakers and participants who attended the SAB Integrated Nitrogen
Committee workshop held on October 20-22, 2008.  These participants included: the following individuals from the
U.S. EPA - Mr. Richard Batiuk, Dr. Jana Compton, Dr. Robin Dennis, Ms. Katie Flahive, Dr. Richard Haeuber, Ms.
Chris Lewiciki, Dr. James Liebman, Ms. Sally Shaver, Ms. Roberta Parry, Mr. Randy Waite, Mr. John Wilson, Mr. Tom
Wirth, and Mr. Marcus Zobrist; the following individuals from the USDA - Dr. Dan Jaynes, Dr. Ray Knighton, and Mr.
Richard  Swenson; and the following individuals from other organizations - Dr. Jan Willem Erisman, Energy Research
Centre of the Netherlands; Mr. Albert Ettinger, Environmental Law and Policy Center; Mr. John Hardin, John Hardin
& Son; Dr. She lie Miller, University of Michigan; Dr. Catherine O'Connor, Metropolitan Water Reclamation District of
Greater Chicago; Mr. Robin O'Malley, The Heinz Center; Mr. Richard Poirot, Vermont Agency of Natural Resources;
Mr. John Quinn, Constellation Energy; Mr. Pat Rice, Nebraska Department of Environmental Quality; Dr. Joe  Rudek,
Environmental Defense Fund; Dr. Ted Russell, Georgia Institute of Technology; Ms. Mindy Selman, World Resources
institute; Dr. Thomas Simpson, University of Maryland; Dr. Richard  Smith,  U.S. Geological Survey; Dr. Clifford Snyder,
International Plant Nutrition Institute; Dr. Robert Summers, Maryland Department of the Environment; Dr. David
Whitall,  National Oceanic and Atmospheric Administration; Ms. Marcia Willhite, Illinois Environmental Protection
Agency; and Mr. James Wurtz, Railway Equipment Company.
   In addition, the Committee thanks the experts who provided independent review of the report in draft form: Dr.
John Day, Louisiana State University; Dr. Elisabeth Holland, National Center for Atmospheric Research; Dr. Gregory
Mclsaac, University of Illinois; Dr. Jerry Melillo, Marine Biological  Laboratory, Woods Hole, Massachusetts;  Dr. Gyles
Randall, University of Minnesota; Dr. James Schauer, University of Wisconsin-Madison; and Dr. Stuart Weiss, Creekside
Center for Earth Observations. The reviewers provided many constructive comments  and suggestions, but were not,
however, asked to endorse the conclusions or recommendations in the report.

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                                                 NOTICE
   This report has been written as part of the activities of the EPA Science Advisory Board (SAB), a public advisory
group providing extramural scientific information and advice to the Administrator and other officials of the Environmental
Protection Agency. The SAB is structured to provide balanced, expert assessment of scientific matters related to problems
facing the Agency. This report has not been reviewed for approval by the Agency and, hence, the contents of this report
do not necessarily represent the views and policies of the Environmental Protection Agency, nor of other agencies in
the Executive Branch of the Federal government, nor does mention of trade names of commercial products constitute a
recommendation for use. Reports of the SAB are posted on the EPA website at http://www.epa.gov/sab.

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Table  of Contents
List of Figures	xi
List of Tables	xiii
List of Boxes	xiv
List of Chemical Abbreviations	xv
List of Acronyms and Abbreviations	xvi
Executive Summary	ES-1
1. Introduction	3
 1.1.  Overview of the Problem - Impacts of Excess Reactive Nitrogen (Nr)
      on Human Health and the Environment	3
 1.2.  The Nitrogen Cascade - Nr Loading, Cycling, and Exposure	3
 1.3.  EPA Activities to Manage Risks Posed by Nr	6
 1.4.  SAB Integrated Nitrogen Committee Study Objectives	7
 1.5.  Study Approach and Structure of the Report	8
2. Sources, Transfer, and Transformation of Nr
  in Environmental Systems	11
 2.1.  Nr Flux in the Environment	11
 2.2.  Sources of New Nr to the Environment	13
   2.2.1.  Nr Formation and Losses to the Environment from Fossil Fuel Combustion	14
   2.2.2.  Nr Inputs and Losses to the Environment from Crop Agriculture	15
   2.2.3.  Nr Inputs and Losses from Animal Agriculture	25
   2.2.4.  Nr Inputs to Residential and Recreational Turf Systems	29
 2.3.  Nr Transfer and Transformations in and Between Environmental Systems	31
   2.3.1.  Input and Transfers ofNr in the United States	31
   2.3.2.  Storage ofNr Within Terrestrial Environmental Systems	35
   2.3.3.  Areas of Uncertainty in Nr Transfer and Transformation	38
3. Impacts of Nr on Aquatic, Atmospheric, and Terrestrial Ecosystems	41
 3.1.  Impacts on Drinking Water, Human Health, and Freshwater Biota	41
 3.2.  Impacts of Airborne or Atmospherically Deposited Nr on Human Health
      and Ecosystems	42
 3.3.  Impacts ofNr on Terrestrial Ecosystems	42
4. Metrics and Current Risk Reduction Strategies for Reactive Nitrogen	43
 4.1.  Measurement ofNr in the Environment	43
 4.2.  Consideration ofNr Impacts in Risk Reduction Strategies	43
 4.3.  Water Quality Regulation and Management	45
 4.4.  Water Quality Monitoring and Assessment	49
 4.5.  Clean Air Act and Air Quality Regulation and Management	50
 4.6.  Thresholds for Excess Nr Effects on Terrestrial Ecosystems	52
 4.7.  Comments on Nr Critical Loads	52
 4.8.  Tradeoffs ofNr Impacts in Risk Reduction Strategies	53
 4.9.  Interactions of the N Cascade and Climate	56

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5. Integrated Risk Reduction Strategies for Nr	59
 5.1.  Importance of Integrated Risk Reduction Strategies	59
 5.2.  Control Strategies for Nr	59
 5.3.  Management Strategies for Nr in the Environment	59
   5.3.1.   Command-and-control	59
   5.3.2.   Direct Allocation of Federal Funds for Conservation Programs	60
   5.3.3.   Market Based Instruments for Pollution Control	61
   5.3.4.   Biophysical and Technical Controls (control points) on Transfer
          and Transformations ofNr in and Between Environmental Systems	62
6. SAB Recommendations for Nr Data Collection, Risk Management, and Research	73
 6.1.  Need for Comprehensive Monitoring of Nr	73
 6.2.  Overarching Recommendations	74
 6.3.  Near-term Management Goals	75
 6.4.  Summary of Specific Findings and Recommendations
      Corresponding to the Four Study Objectives	79
 6.5.  Conclusions and Observations	85
Appendix A: Nitrogen Deposition From the Atmosphere to the Earth's Surface	89
Appendix B: Sources and Cycling of Nr Input
            into Terrestrial Systems in the United States	95
Appendix C: Water Quality Trading in the Illinois River Basin	99
Appendix D: Management of Nr Measures Based
            on the Concept of Critical Loads	103
Appendix E: Technical Annexes	107
Appendix F: Recent Major EPA Mobile Source Rules to Control NOX	109
Appendix G: Impacts of Reactive Nitrogen on Aquatic Systems	Ill
Appendix H: Nr Saturation and Ecosystem Function	121
References	123

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List  of  Figures
Figure ES-1: The nitrogen cascade	ES-3
Figure ES-2: Sources of reactive nitrogen (Nr) introduced into the United States in 2002 (Tg N/yr)	ES-5
Figure 1: The nitrogen cascade	5
Figure 2: Sources of reactive nitrogen (Nr) introduced into the United States in 2002 (Tg N/yr)	11
Figure 3: U.S. NOX emission trends, 1970-2006	14
Figure 4: Percent reductions in NOX emissions, 1990-2002, from different sources
         (off-road, on-road, power generation, etc.)	14
Figure 5: Mobile source NOX emission inventories	15
Figure 6: Fertilizer consumption in the United States, 1960 to 2006	17
Figure 7: Trends in corn grain produced per unit of applied fertilizer N (NFUE) in the United States	19
Figure 8: Synthetic fertilizer and livestock manure N used as fertilizer in Denmark 1985-2003	21
Figure 9: Protein content of cereal grain in Denmark	21
Figure 10: Meat production from 1970 to 2006	25
Figure 11: Milk production from 1970 to 2006	25
Figure 12: U.S. inventory of mature dairy cows and milk production per cow from 1970 to 2006	26
Figure 13: Number of animal operations in the United States from 1970 to 2006	26
Figure 14: Nr input and loss from 16 watersheds in the northeast United States	39
Figure 15: Relative importance of all reactive nitrogen sources released into atmospheric, terrestrial,
          and freshwater media within the Chesapeake Bay Watershed utilizing four different metrics	46
Figure 16: Quantified damage costs (including health impacts) relative to tonnes of reactive nitrogen
          in Chesapeake Bay Watershed	46
Figure 17: Diagram of the nitrification and denitrification processes	54
Figure 18: Combined carbon and nitrogen global cycles	55
Figure 19: Comparisons between Global Warming Potential (GWP) and eutrophication impact
          categories for various bioproducts	56
Figure 20: Probability of given discharge level for nitrate in the watersheds of eastern Iowa	57
Figure 21: Relative nitrogen discharge (Ibs/day) from 79 POTWs	64
Figure 22: Trading ratios for municipalities in Connecticut	65
Figure 23: The likely impact of research investment in increasing N fertilizer use efficiency	68
Figure A-l: Percent change in relative contribution of oxidized (NCV) and reduced (NH4+)
           nitrogen wet deposition from 1994 to 2006	89
Figure A-2: Trend in reported wet deposition of NH4+ and NO3~ for the 48 contiguous  states	90
Figure A-3: Annual NH4+, NCV, and total inorganic N deposition for the year 2007 showing
           spatial patterns of deposition	90
Figure A-4: CMAQ annual average (wet plus dry and oxidized plus reduced) nitrogen deposition
           (in kg-N/ha/yr) across the United States	93
Figure C-l: Distribution of municipal discharge, and industrial dischargers in the
           Illinois River Watershed	100
Figure C-2: Distribution of total nitrogen emissions by sub-watershed	100
Figure C-3: Potential land availability in the  100-year flood zone for nutrient farming in each
           sub-watershed in the Illinois River Watershed	100
Figure C-4: Spring available total nitrogen load by sub-watershed	100

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Figure C-5: Spring marginal cost (price) by watershed	101
Figure C-6: Unrestricted spring credit sales (tons/month) by sub-watershed	101
Figure E-l: Total Nryields (kg/ha/yr) in large rivers of the U.S	108
Figure G-l: U.S. Population (1995) supplied by domestic drinking water wells	112
Figure G-2: Nitrate concentrations in U.S. domestic drinking water wells	113

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List  of  Tables
Table ES-1: Examples of impacts of excess reactive nitrogen on human health and environment	ES-2
Table 1: Nrfluxes for the United States, TgN in 2002	12
Table 2: Examples of multiple sources from states with high NOX emissions based on 2001 data;
        and tons of NOX as NO2	15
Table 3: Types and amount of nitrogen fertilizers used in the United States in 2002	18
Table 4: Estimates of nitrogen input from biological nitrogen fixation from major legume crops,
        hay, and pasture	22
Table 5: N2O emissions in the United States, 2002	23
Table 6: Livestock N excretion per kg production (g/kg) and per total United States (Tg/yr)	28
Table 7: Manure production from animal husbandry in the continental United States, Tg N per year 2002	28
Table 8: Fate of livestock manure nitrogen (Tg N)	29
Table 9: Estimate of fertilizer N used on turf grass in the United States in the year 2000, based on
        total area of 12.6 million ha	30
Table 10: Net annual change in continental U.S. croplands soil N and C, forest C and N, and
         grasslands C and N in 2002	37
Table 11: Ecosystem service and corresponding function categories	44
Table 12: Marginal abatement cost per tonne of Nr by source	47
Table 13: Federal primary ambient air quality standards that involve Nr, effective February 2010	50
Table 14: Advantages and limitations of various approaches to Nr control in forestry and agriculture	60
Table 15: Summary of market-based instruments for pollution control with conceptual examples	63
Table 16: Performance of the Nitrogen Credit Exchange	64
Table 17: Estimates for potential decreases inNH3 emissions from livestock manure in the United States	78
Table A-l: Annual wet deposition of reduced (NH4+), oxidized (NO3~), and total N to the
          48 contiguous states	89
Table A-2: Deposition of N to the eastern United States in units of kg N/ha/yr	91
Table A-3: Results from CMAQ for total deposition in 2002 to the 48 contiguous states of oxidized
          and reduced N	92
Table B-l: Sources of reactive N into terrestrial systems in the United States in 2002
          (from Table 1 data sources; in Tg N/yr)	95
Table B-2: Nr input and flows (Tg N/yr) in the terrestrial portion of the Nitrogen Cascade
          within the continental United States in 2002	96
Table C-l: Nutrient farm market parameters under three trading scenarios	101
Table D-1: Summary of the effects of excess Nr on human health in relation to metrics, current
          international regulations and conventions, and the link to the nitrogen cascade	104
Table D-2: Summary of the effects of excess Nr on ecosystems related to currently used metrics,
          the existence of European regulatory values, and the link to the nitrogen cascade	105
Table D-3: Summary of the effects of excess N on other societal values in relation to metrics and
          regulatory values in current international regulations and conventions and the link to
          the nitrogen cascade	106
Table G-l: Estuaries with nitrogen management plans or TMDLs and percent nitrogen load reduction targets.... 118

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List  of Boxes
Box 1: Hypoxia in the Gulf of Mexico	35
Box 2: Economic impact and metrics for Chesapeake Bay and its watershed	46
Box 3: The impact of climate change on agricultural discharge of reactive nitrogen	57
Box 4: Water quality trading to meet the Long Island Sound wasteload allocation in Connecticut	63
Box G-l: The national criterion for ammonia in fresh water	114
Box G-2: Long Island Sound total maximum daily load: focus on reactive nitrogen	119

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List  of  Chemical Abbreviations
C             Carbon
CFC          Chlorofluorocarbon
DIN          Dissolved inorganic nitrogen
DO           Dissolved oxygen
Fe            Iron
H             Hydrogen
HNO3         Nitric acid
HONO         Nitrous acid
N             Nitrogen
N2            Diatomic (molecular) nitrogen
N2O          Nitrous oxide,
N2Os          Dinitrogen pentoxide (nitric acid anhydride)
NH3          Ammonia
NH4+          Ammonium
NHX          NH3 NH4+
NO           Nitric oxide
NO2          Nitrogen dioxide
NO3"          Nitrate ion
NO3          Nitrate radical
Norg          Organic nitrogen
NOX          Nitrogen oxides (NO + NO2)
NOy          Total reactive oxidized nitrogen
              (NO, NO2, NO3, 2xN2O5, HONO, HNO3, NO3', PAN and other organo-nitrates, RONO2)
Nr            Reactive nitrogen
O2            Oxygen
OH           Hydroxyl radical
P             Phosphorus
PAN          Peroxy acetyl nitrate
PM           Paniculate matter
PM2 5          Particulate matter less than 2.5 microns in diameter
PM10          Particulate matter less than 10 microns in diameter
RONO2        Organic nitrates
Si             Silicon
SO2           Sulfur dioxide
SO42-          Sulfate
TAN          Total ammonical nitrogen

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AAPFCO       Association of American Plant Food Control Officials
AARA         American Reinvestment and Recovery Act
AIRMON       Atmospheric and Integrated Research Monitoring Network
AOB           Ammonia oxidizing bacteria
BL            Boundary layer
BMP           Best management practice
BNF           Biological nitrogen fixation
BNR           Biological nutrient (or nitrogen) removal
CAA           Clean Air Act
CAFO         Concentrated animal feeding operation
CAIR          Clean Air Interstate Rule
CALM         Consolidated Assessment and Listing Methodology
CAST         Council for Agricultural Science and Technology
CASTNET     Clean Air Standards and Trends Network
C-BNF         Cultivation-induced biological nitrogen fixation
CCC           Criterion continuous concentration
CFC           Chlorofluorocarbon
CFR           Code of Federal Regulations
CL            Critical load (threshold of Nr loading at which negative impacts have been documented)
CLAD         Critical Loads Ad-Hoc Committee
CMAQ         Community multiscale air quality
CMC           Criterion maximum concentration
CRP           Conservation Reserve Program
CSO           Combined sewer overflow
CTM           Chemical Transport Models
CWA           Clean Water Act
CWSRF        Clean Water State Revolving Fund (construction grants program under the Clean Water Act)
DOE           U.S. Department of Energy
DOT           U.S. Department of Transportation
EGU           Electricity generating units
EFD           Essential Facilities Doctrine
EGR           Exhaust gas recirculation
EISA           Energy Independence and Security Act
EPA           United States Environmental Protection Agency
EQIP           Environmental Quality Incentives Program
EU            European Union
FAO           Food and Agricultural Organization of the United Nations
FAOSTAT      Food and Agricultural Organization Statistical Database
FOR           Flue-gas recirculation

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ha             Hectare
GHG          Greenhouse gas
GPS           Geographic Positioning System
HAB          Harmful algal bloom
IPCC          Intergovernmental Panel on Climate Change
ISA           Integrated Science Assessments
ITQ           Individual transferable quota
kg             Kilogram
L              Liter
LA            Load allocation
LCA          Life cycle analysis
LISS          Long Island Sound Study
MCL          Maximum contaminant level
MCLG         Maximum contaminant level goal
mg            Milligrams
MOD          Million gallons per day
MJ            Megajoule (one million joules)
Mmt          Million metric tons
MT            metric tons
MOM          Mississippi-Ohio-Missouri
MRB          Mississippi River Basin
MS4           Municipal separate storm sewer system
NAAQS        National Ambient Air Quality  Standards
NADP         National Atmospheric Deposition Program
NASS          National Agricultural Statistics Service Information
NCA          National Coastal Assessment
NCE          Nitrogen Credit Exchange
NCCR         National Coastal Condition Report
NEEA         National Estuarine Eutrophication Assessment
NESCAUM      Northeast States for Coordinated Air Use Management
NFUE         Nitrogen fertilizer use efficiency. Calculated as the ratio of grain yield to
               the quantity of applied N fertilizer (kg grain/kg applied N).
NMP          Nutrient management plan
NOAA         National Oceanic and Atmospheric Administration
NFS           Nonpoint source
NRC          National Research Council
NRCS         Natural Resources Conservation Service
NRD          Natural Resource District
NRI           National Resources Inventory
NTN          National Trends Network

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NUE            Nitrogen use efficiency. NUE is defined as the kg grain produced
                per kg of total N used by the crop, where total N includes N from fertilizer,
                biological N fixation, and soil organic matter mineralization
OTAG          Ozone Transport Assessment Group
OTC            Ozone Transport Commission
PE              Physiological efficiency (physiological efficiency with which the N
                taken up by the crop is used to produce economic yield such as grain or fruit,
                quantified by kg increase in economic yield per kg of N accumulation in above ground crop biomass)
PFP            Partial factor productivity
POTW          Publicly owned treatment works
PSD            Prevention of significant deterioration
RE              Recovery efficiency (kg N uptake per kg N applied)
SAV            Submerged aquatic vegetation
SNCR          Selective non-catalytic reduction
SCR            Selective catalytic reduction
SIP             State Implementation Plan
SOM            Soil organic matter
SPARROW      Spatially Referenced Regressions on Watershed Attributes Model
STP            Sewage treatment plant
SW             Storm water
SWAT          Storm Water Assessment Tool
SWPPP         Stormwater Pollution Prevention Plan
T               Temperature
Tg              Teragram (million metric tons or 1012 grams)
TMDL          Total maximum daily load
TN              Total nitrogen
UFTRS         Uniform Fertilizer Tonnage Reporting System
UNECE         United Nations Economic Commission for Europe
USDA          U.S. Department of Agriculture
USGS          U.S. Geological Survey
USEPA         United States Environmental Protection Agency
WHO           World Health Organization
WLA           Wasteload allocation
WPCA          Water pollution control authorities
WRI            World Resources Institute
WRP            Wetland Reserve Program
WS A           Wadeable Stream Assessment

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Executive  Summary


Introduction
   Nitrogen (N) is an integral component of all proteins,
which are the basic building blocks of life and catalysts
for life-sustaining reactions in organisms. Reactive
nitrogen (Nr), in contrast to non-reactive gaseous N2,
includes all biologically active, chemically reactive, and
radiatively active nitrogen compounds in the atmosphere
and biosphere of the earth.1 Without an adequate supply
of N in any organism's diet, it can't survive. Ironically,
bioavailable N for nutrition is in short supply and indeed
the productivity of most of the world's ecosystems is
often limited by the availability of N. This is certainly
the situation with food production. Without the creation
of N fertilizer by an industrial process (the Haber-Bosch
process) and the increased cultivation of leguminous
crops, the world could not support the current human
population or its projected increase.
   As further discussed in Chapter 2 of this report,
increased anthropogenic input of Nr to the environment
has contributed to large increases in the mass flux of
nitrogen via the nitrogen cycle. Anthropogenic sources
of N now provide enough N, on average, to grow food
for the world's peoples. However, a major consequence
of this nearly inexhaustible supply is that most N used
in food production, and all of the new Nr produced by
fossil fuel combustion, is lost to the environment where
it circulates through the earth's atmosphere, hydrosphere,
geosphere, and biosphere. During this circulation, Nr
contributes to a wide variety of consequences, which
are magnified with time as Nr moves through the
environment.

Impacts of reactive nitrogen on  human
health  and the environment
   Anthropogenic creation of Nr provides essential
benefits for humans - first and foremost in meeting
human dietary  needs. A large fraction of the human
population of the earth could not be sustained if synthetic
nitrogen fertilizers did not significantly augment food
production. Essentially all of the Nr created by human
activities, however, is released to the environment, often
with unintended negative consequences. As summarized
in Table ES-1,  it contributes to a number of adverse
public health and environmental effects, including
photochemical smog, decreased atmospheric visibility,
acidification of terrestrial and aquatic ecosystems,
eutrophication of coastal waters (i.e., harmful algal
blooms, hypoxia), drinking water concerns, freshwater
Nr imbalances, greenhouse gas emissions and subsequent
climate change, and stratospheric ozone depletion.
   In light of the magnitude of the human alteration of the
nitrogen cycle, and the resulting negative consequences
on humans and ecosystems, the National Academy of
Engineering has identified management of the nitrogen as
one of the "grand challenges" facing this country.2
   Nr effects are manifest as direct declines in both
human health (e.g., respiratory and cardiac diseases) and
ecosystem health (e.g., coastal eutrophication and loss in
biodiversity). In addition, there are indirect declines in
human health because the negative impacts on ecosystems
will diminish the services that those  ecosystems provide
people. The effects are often magnified because the
same atom of nitrogen can cause multiple effects in
the atmosphere, in terrestrial ecosystems, in freshwater
and marine systems, and on human health. We call this
sequence of effects the nitrogen cascade.

The nitrogen cascade
   The nitrogen cascade has three dimensions:
biogeochemical, alterations in the environment, and
human and ecosystem consequences.
   The "biogeochemical" dimension of the nitrogen
cascade  involves: Nr creation from N2 as a consequence
of chemical, food, and energy production; Nr use in food
and chemical production; Nr losses to the environment;
changes in Nr species residence times in environmental
reservoirs; Nr transfers among reservoirs; and Nr
conversion back to N2. Alterations to the environment
then result from increased Nr levels  in the environment.
These alterations have negative consequences for
ecosystem and human health at local, regional, national,
and global scales. Because nitrogen is a critical resource
and also a contributor to many of the environmental
concerns facing the U.S. today, it is imperative to
understand how human action has altered N cycling in the
U.S., and the consequences of those  alterations on people
and ecosystems. The overarching question is, how do
we protect and sustain ecosystems that provide multiple
benefits  to society while also providing the interconnected
material, food and energy required by society?
1 Reactive nitrogen (Nr) includes inorganic chemically reduced forms of N (NHX) [e.g., ammonia (NH3) and ammonium ion (NH4+)], inorganic
 chemically oxidized forms of N [e.g., nitrogen oxides (NOX), nitric acid (HNO3), nitrous oxide (N2O), ^05, HONO, peroxy acetyl compounds such
 as peroxyacytyl nitrate (PAN), and nitrate ion (NC>3~)], as well as organic compounds (e.g., urea, amines, amino acids, and proteins).

2 National Academy of Engineering Grand Challenges (http://www.engineeringchallenges.org/cms/challenges.aspx)

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Table ES-1: Examples of impacts of excess reactive nitrogen on human health and environment
Impact

Location Metric
Source Reference

Visibility decrease
Fine participate
matter
National Parks and
wilderness areas
visibility impairment
NOy and NHxfrom
fossil fuels and
agriculture
Malm et al.,
2004; U.S.
EPA Clean Air
Scientific Advisory
Committee,
2004 EPA-
CASAC-09-01 0
LAND - ECOSYSTEMS
Biodiversity loss
Forest decline
Nitrogen deposition
Ozone and acid
deposition
Grasslands and
forests in the United
States receiving N
deposition in excess
of critical load
Eastern and West-
ern United States
Decrease in species
richness of grass-
lands and forests
Decreased timber
growth; increased
susceptibility to
disease and pests
Utilities, traffic, and
animal agriculture
Utilities, traffic, and
animal agriculture
Bobbink et al.,
2010; Fennetal.,
2003.
Johnson &
Siccama, 1983;
MacKenzie &
EI-Ashry, 1990

Crop yield loss
Ozone
Eastern and West-
ern United States
$ 2-5 billion/year
Utilities & traffic
Hecketal., 1984
WATER
Acidification of
surface waters; loss
of biodiversity
Hypoxia of coastal
waters
Harmful Algal
Blooms
Acidification of
soils, streams and
lakes is caused
by atmospheric
deposition of
sulfur, HNO3, NH3
and ammonium
compounds.
Excess nutrient
loading, eutrophi-
cation, variable
freshwater runoff
Excessive nutrient
loading, climatic
variability
Primarily mountain-
ous regions of the
United States
Gulf of Mexico,
other estuarine and
coastal waters
Inland and coastal
waters
Out of 1 ,000 lakes
and thousands of
miles of streams
in the Eastern
United States
surveyed, 75% of
the lakes and 50%
of the streams were
acidified by acid
deposition
Benthic finfish/shell-
fish habitat loss, fish
kills, sulfide toxicity,
costs >$50 million
annually
Fish kills, losses
of drinking and
recreational waters
costs >$100 million
annually
Fossil fuel combus-
tion and agriculture
N, P from energy
and food production
Excess nutrient
(N & P) loading
U.S. EPA, 2008a
http://www.epa.
gov/acidrain
Bricker et al.,
1999; Verity et
al., 2006; U.S.
EPA SAB, 2007;
Rabalais et al.,
1999; Mitschet
al., 2001
Paerl, 1988;
ECOHAB, 1995;
NRC, 2000
HUMAN AND ENVIRONMENTAL HEALTH DAMAGES
Human mortality
Total damage to
public health and
environment
Total damage to
public health and
environment
PM2.5, O3and
related toxins.
NOX into air
NHX and nitrate into
air and water
U.S. urban and
nearby areas
Chesapeake Bay
Watershed
Chesapeake Bay
Watershed
Pollution related
deaths estimated
at 28,000-55,000
per year (a range
of cardiovascular
and respiratory
system effects are
associated with this
pollution).
$3.4 Billion;
200,000 MT
$1.5 Billion;
400,000 MT
NOy and NHxfrom
fossil fuels and
agriculture
Mobile sources
Agriculture
Mokdad et al.,
2004; Ezzati et
al., 2004.
Moomawand
Birch, 2005; Birch
etal.,2011
Moomawand
Birch, 2005

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   Nr inputs to the nation and the world have been
increasing, largely due to human activities associated
with food production and fossil fuel combustion. Despite
the obvious benefits of a plentiful supply of food and
energy, the adverse consequences associated with the
accumulation of Nr in the environment are large, with
implications for human health and the environment.
   The greater the inputs of Nr to the landscape, the
greater the potential for negative effects caused by
greenhouse gas (GHG) production, ground level ozone.
acid deposition, and Nr overload that can contribute
to climate change, degradation of soils and vegetation.
acidification of streams, lakes and rivers, estuarine and
coastal eutrophication, hypoxia, and habitat loss.
   The growing nature of the Nr problem, and the adverse
and intertwined consequences associated with Nr inputs
to air, land, and water as exhibited in the N cascade
underscore the need for researchers and managers to
explore integrated strategies that minimize N inputs.
maximize its use efficiency, promote Nr removal
processes, and protect humans and natural resources.
   The concept of the nitrogen cascade highlights that
once a new Nr molecule is created, it can, in sequence.
travel throughout the environment contributing to major
environmental problems (Galloway et al., 2003). The
         adaptation of the cascade in Figure ES-1 was developed
         by the SAB Integrated Nitrogen Committee (INC)
         to provide a context for considering nitrogen-related
         issues and ecosystem effects in the U.S. To consider
         the cascading effects of Nr in the U.S., we examined
         the various atmospheric, terrestrial, and aquatic
         environmental systems where Nr is stored, and the
         magnitudes of the various flows of N to, from, and within
         them. The nitrogen cascade concept implies the cycling
         of Nr among these systems. The process of denitrification
         is the only mechanism by which Nr is converted to
         chemically inert N2, "closing" the continuous cycle
         (Figure ES-1 shows only flows of reactive nitrogen, not
         N2). Denitrification can occur in any of the indicated
         reservoirs except the atmosphere.
            The "new" N box in the Nitrogen Cascade depicts
         the two primary anthropogenic sources by which Nr
         originates - energy production and food production -
         and where Nr from these sources enters ecosystems.
         Energy production includes both fossil fuel and biofuel
         combustion. Food production includes N fertilizer
         produced in the U.S., cultivation-induced biological N
         (C-BNF) in the U.S., production of animals and crops
         in the U.S. for human consumption, and imports of
         N-containing fertilizer, grain and meat to the U.S.
The Nitrogen Cascade
        New Nitrogen
        Energy Production     NOX
        & combustion of fossil fuels
        Food Production      NMx
        & creation of synthetic fertilizers
                            N0y, NHX, Norg
Atmospheric
                       NOy, NHX, Norg
Terrestrial
                                                    Agricultural
                                                  crops
              animals
  t-J
                                                        soils
                               Vegetated
                                                                           forests     grasslands
                                                                          t-J   soils  L-t
                               Populated
                                                                          people  I-W landscape
                                                                                 S°ilS
                                                   NOX
                                                   NH3
                                                   Norg
                                                                                                   N20
                        N0y, NHX, Norg
                                               Aquatic
                                                                                                   N2O
Figure ES-1: The nitrogen cascade

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   The atmospheric system box in the Figure ES-1
indicates that tropospheric concentrations of both ozone
and paniculate matter are increased due to emissions
of nitrogen oxides3 (NOX) to the atmosphere. The ovals
illustrate that the increase in N2O concentrations, in turn,
contribute to the greenhouse effect in the troposphere and
to ozone depletion in the stratosphere. Except for N2O,
there is limited Nr storage in the atmosphere. Losses of
Nr from the atmospheric system include total oxidized
nitrogen4 (NOy), reduced nitrogen5 (NHX), and organic
nitrogen (Norg) deposition to terrestrial and aquatic
ecosystems of the earth's surface. There is little potential
for conversion of Nr to N2 via denitrification in air.
However, once airborne deposition of Nr occurs it will be
subject to denitrification pathways via soil and water.
   The terrestrial system box in the Figure ES-1 depicts
that Nr enters agricultural lands via food production
and is introduced to the entire terrestrial landscape via
atmospheric deposition. Within agricultural regions there
is cycling among soils, crops and animals, and then a
transfer of Nr as food to populated regions, from which
there are Nr losses to the environment (e.g., sewage,
landfills). The ovals showing ecosystem productivity
and biogeochemical cycling reflect that Nr is actively
transported and transformed within the terrestrial system,
and that as a consequence there are significant impacts
on ecosystem productivity due to fertilization and
acidification, often with resulting losses of biodiversity.
There is ample opportunity for Nr storage in both biomass
and soils. Losses of Nr from this system occur by leaching
and runoff of NOy, NHX and Norg to aquatic ecosystems
and by emissions to the atmospheric system as NOX, NH3,
Norg, and N2O. There is potential for conversion of Nr to
N2 via denitrification in the terrestrial  system.
   The aquatic system box in the Figure ES-1 shows that
Nr is introduced via leaching and runoff from terrestrial
ecosystems and via deposition from atmospheric
ecosystems.  Connected with the hydrological cycle,
there are Nr fluxes downstream with ultimate transport
to coastal systems. Within the aquatic  system, the
ovals highlight two significant impacts of waterborne
Nr acidification of freshwaters and eutrophication of
fresh and coastal waters. Except for Nr accumulation in
groundwater reservoirs, there is limited Nr storage within
the hydrosphere. Losses of Nr from the aquatic system are
primarily via N2O emissions to the atmospheric system.
There is a very large potential for conversion of Nr to N2
via denitrification in water and wetlands.
   NOy, NHX and N2O are all components of Nr, but
a fundamental difference is that the NOy and NHx are
rapidly transferred from the  atmosphere to receiving
ecosystems due to a short atmospheric residence time
(< 10 days) where they continue to contribute to the
N cascade. Because of its longer residence time (-100
years) however, N2O remains in the troposphere where it
contributes to climate change, until it is transferred to the
stratosphere, where it contributes to ozone depletion.

Trends in N  inputs to the United States
   In 2002, humans introduced 29 teragrams (Tg) of
newly formed reactive N into the U.S. through Haber-
Bosch process production of fertilizers and industrial
Nr, cultivation-induced biological nitrogen fixation (i.e.,
conversion of N2 to NH3 by microorganisms associated
with some cultivated crops, for example, legumes), and
fossil fuel combustion (Figure ES-2). By definition, prior
to human presence in the U.S., there was no introduced
anthropogenic Nr. Prior to 1900, no Haber-Bosch Nr
was introduced, fossil fuel combustion introduced very
small amounts relative to today, and cultivation-induced
biological nitrogen fixation created approximately 2
Tg N. Thus, between 1900 and  2002, the amount of Nr
introduced to the U.S.  has increased by approximately
10-fold.

Nitrogen inputs  to the United States
   The EPA Science Advisory Board (SAB) Integrated
Nitrogen Committee ("Committee") evaluated nitrogen
inputs to the U.S. in 2002. At the global scale, human
activities produced approximately twice as much Nr as
did natural processes. In the U.S., however, the amount of
Nr produced by human activities was approximately five
times larger than natural processes. As shown in Figure
ES-2, natural ecosystems in the U.S. introduce about 6.4
Tg of Nr as N per year (Tg N/yr). In contrast, human
activities introduce about 28.5 Tg N/yr.
   Chapter 2 of this report discusses sources, transfer,
and transformation of Nr. Supporting references for
the information presented on this topic are presented in
Chapter 2. The largest single source of Nr in the U.S. is
the Haber-Bosch process, which introduces about 15.2
Tg N/yr: 9.4 Tg N/yr from domestic Nr production and
5.8 Tg N/yr from imports of Nr in fertilizers. The 15.2
Tg N/yr of anthropogenic Nr is used in three ways: 9.9
Tg N/yr is used to produce agricultural crops; 1.1 Tg N/
yr is applied to turf grasses; and 4.2 Tg N/yr is used by
industry for production of nylon, refrigerants, explosives
and other commercial products.
   The second largest source of Nr introduced into the
U.S. is enhancement of biological nitrogen fixation (BNF)
by cultivation of legumes like soybeans and alfalfa that
have nitrogen-fixing symbionts, or by crops like rice
that have nitrogen-fixing bacteria in their rhizosphere.
These Nr fixing crops introduce about 7.7 Tg N/yr. A
3 NOx (oxides of nitrogen) includes NO + NO2
4 NOy (total oxidized nitrogen) includes NO, NO2, NO3, N2O5, HONO, HNO3, NO3-, PAN and other organo-nitrates, RONO2
5 NHx (reduced nitrogen) includes NH3 + NH4

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                                      Natural
         Industry
    Nonfertilizer Haber Bosch N
                          Transportation

              Fossil Fuel
                                                                       Haber Bosch N Fertilizer
                               Cultivation BNF
                                                  Agriculture

Figure ES-2: Sources of reactive nitrogen (Nr) introduced into the United States in 2002 (Tg N/yr).

Figure ES-2 explanatory notes:
 Numerical units: teragram of reactive nitrogen (Nr) per year (Tg N/yr)
 Natural BNF: biological nitrogen fixation in natural grasslands, rangelands, and forests.
 Fossil Fuel-Transportation: combustion in vehicles, trains, airplanes, ships and off-road construction equipment.
 Fossil Fuel-Stationary: combustion of fossil fuels in power plants and industrial boilers.
 Agriculture-cultivation BNF: agricultural augmentation of biological nitrogen fixation - for example by planting of
 nitrogen fixing legumes.
 Agriculture-Haber Bosch N fertilizer: agricultural (including turf production) use of synthetic nitrogen fertilizers
 produced by the Haber Bosch process for converting gaseous N2 to Nr.
 Industry-Haber Bosch N: Industrial sources of Nr produced by the Haber-Bosch process.
 Figure ES-2 documents only the introduction of new Nr in the United States, and not the transfers of existing Nr among
 systems (e.g., Nr in manure).
small amount of additional Nr is also imported in grain
and meat products; in 2002 this source of added Nr was
approximately 0.2 Tg N/yr (not shown in Figure ES-2).
   Fossil fuel combustion is the third largest source of
new Nr. It introduces approximately 5.7 Tg N/yr into
the environment (almost entirely as NOX), that is, 3.8 Tg
N/yr from transportation sources and 1.9 Tg N/yr from
stationary sources such as electric utilities, industrial
boilers and from certain industrial processes.
   In summary, agriculture and domestic use of fertilizers
to produce food, feed, and fiber (including bioenergy
and BNF) and combustion of fossil fuels are the largest
sources of new Nr released into the environment in the
U.S. The percentage distribution of Nr released to the U.S.
environment from human activities in 2002 was: about
65% from agricultural sources (including BNF and turf
production), about 20% from fossil fuel sources, and about
15% from industrial sources (Figure ES-2).

Distribution of reactive nitrogen through
the environment
   Once introduced into the U.S., Nr compounds are
distributed via the atmosphere, hydrosphere, geosphere.
biosphere, and commerce. Distribution in the atmosphere
begins with NOX, NH3, and N2O. NOX and NH3 (and their
reaction products) are distributed on a scale of hundreds
to thousands of kilometers within the U.S. boundaries.
and also distributed to downwind countries and oceans.
Due to its long lifetime (approximately 100 years) in the
atmosphere, N2O accumulates in the U.S. atmosphere

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and is also dispersed throughout the global atmosphere.
All ecosystems in the conterminous U.S. receive
anthropogenic Nr from the atmosphere and for many
ecosystems it is their primary, albeit unintended, source of
Nr. Once deposited, Nr can be stored in soils and biomass
and widely distributed via the stream-river continuum to
inland and coastal waters. Some of the Nr is converted to
N2O or denitrified to N2, primarily in aquatic ecosystems,
including wetlands. Commerce is a major mechanism that
transfers Nr from one place to another in the U.S.; most of
the Nr that is used to produce food (e.g., fertilizer) and in
food products crosses state boundaries via roads, railroads
and the air.
   Putting values to this distribution, of the  6.3 Tg N/
yr of U.S. NOX emissions, 2.7 Tg N/yr are deposited
back onto the land and surface waters of the U.S. Thus,
by difference we estimate that as much as 3.6 Tg N/
yr of the U.S. NOX emissions are advected out of the
U.S. via the atmosphere. Similarly, of the 3.1 Tg N/yr
of NH3 that are emitted into the U.S. atmosphere each
year, about 2.1 Tg N/yr are deposited onto the land
and surface waters of the U.S., and about 1 Tg N/yr is
advected out of the U.S. via the atmosphere. Emissions
of N2O discharge about 0.8 Tg N/yr into the global
atmosphere. In sum, 5.4 Tg N are advected out of the
U.S. from all sources each year either to other nations or
to the global atmospheric or ocean commons.
   Riverine discharges  of Nrto the U.S. coastal zone
account for 4.8 Tg N/yr, while export of N-containing
commodities (e.g., grain) removes another 4.3 Tg N/
yr from the U.S..  Altogether, along with 5.4 Tg N/yr
of atmospheric advection, these total Nr outputs out of
the U.S. continental environment add up to about 14
Tg N/yr, leaving about  21 Tg N/yr unaccounted for. Of
this amount, we estimate that 5 Tg N/yr are stored in
soils, vegetation,  and groundwater and, by difference,
we estimate that about  16 Tg N/yr are denitrified to N2.
Denitrification, a process that microbially converts Nr
to N2 (as well as forming some N2O) requires both a
carbon source and anaerobic conditions, a situation that is
found in wetlands, oxygen-depleted streams, rivers, and
the hypolimnion of reservoirs (or their sediments), soils,
and engineered denitrification systems. This process can
be a major Nr sink in river basins.  There are substantial
uncertainties (+/- 50%) for estimated emission and
deposition and terms that are arrived at by difference (e.g.,
atmospheric advection  and denitrification) - especially
those that involve NHX. The Committee considered
these uncertainties in developing the "Overarching
Recommendations" of this report.

Current EPA Nr risk management and
research programs
   The parts of EPA most directly concerned with
managing or conducting research on Nr are the Office
of Air and Radiation, the Office of Water, and the Office
of Research and Development (ORD). Over a dozen
programs of EPA's Office of Air and Radiation reduce
risks from Nr. These programs and related activities
include: National Ambient Air Quality Standards
standard setting and implementation; emission standards
for industrial stationary sources and area sources; the
Acid Rain Program; the Clean Air Interstate Rule;
and programs that focus on mobile source emissions.
Programs designed to save energy, such as Energy Star,
tend to reduce emissions of Nr as well. EPA's Office of
Water addresses Nr under both the Clean Water Act and
the Safe Drinking Water Act through activities such as:
criteria development and standard setting; total maximum
daily load (TMDL) development; National Pollution
Discharge Elimination System (NPDES) permits;
infrastructure financing through the Drinking Water and
Clean Water State Revolving Funds; watershed planning;
wetlands preservation; and regulation of stormwater and
runoff sources that include municipal separate storm
sewer systems (MS4), and concentrated animal feeding
operations (CAFOs). EPA's Office of Research and
Development aims to conduct leading-edge research and
foster the sound use of science and technology in support
of the Agency's mission. The Office of Research and
Development is well recognized for providing a scientific
basis for the development of the National Ambient Air
Quality  Standards for NOX and paniculate matter (PM).
The Office of Research and Development's Ecosystem
Services Research Program has  been developed to
identify and quantify the positive and negative impacts
on ecosystem services resulting from changes in nitrogen
loadings from major source categories. This research
will support policy and management decisions in EPA's
Offices of Air and Radiation and Water.
  EPA has brought a great variety of risk reduction tools
to bear on Nr: conventional regulation and enforcement;
cap and trade approaches; measurement, monitoring and
place-based approaches; control technology development
and verification; communication and education;
intergovernmental and international cooperation; and
voluntary approaches. The variety  and breadth of EPA
programs addressing Nr reflect the ubiquity of Nr in the
environment, the historical single-medium regulatory
approach, and the lack of a "silver bullet" for reducing
risks from Nr.

Need for an integrated  management
strategy
  The EPA programs discussed above (and the programs
of EPA's predecessor organizations) have been active
in the management of Nr through efforts to: decrease
or transform Nr in sewage; control NOX to decrease
photochemical smog and acid rain; control Nr inputs
to coastal systems; control fine particulates in the
atmosphere; and decrease Nr leaching and runoff from
crop and animal production systems and developed

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lands. As beneficial as those efforts have been, they have
focused on the specific problem without consideration of
the interaction of a particular system with other systems
downstream or downwind. Given the reality of the
nitrogen cascade, this approach may  result in short-term
benefits for a particular system but may only temporarily
delay larger-scale impacts on other systems. Thus there
is a need to integrate N management programs, to ensure
that efforts to lessen the problems caused by N in one area
of the environment do not result in unintended problems
in other areas.
   Biofuels feedstock production provides a good
example of the need for comprehensive and integrated
assessment and management of Nr. Increasing corn
production for ethanol has raised the prospect of increased
Nr losses (i.e., transfer from fertilized land to water) and
degraded water quality. The alternative of cellulosic based
ethanol does not necessarily mitigate the potential for this
negative externality. High yields of cellulosic materials
also require N and the "marginal" land assumed for such
production may be  more susceptible to nutrient leakage.
Another good example is provided in Chapter 4 of this
report (Box 2). This example considers the water impacts
of Nr in the Chesapeake Bay and shows that the total
reduction of damage from excess Nr may rely nearly as
much on stricter enforcement of the Clean Air Act as the
Clean Water Act. This challenges the traditional approach
to regulation, but it is a consequence of comprehensively
examining Nr guided by the nitrogen cascade.
   There can be  many unintended consequences
associated with a focus on managing one pollutant, even
an integrated focus on various forms of N. For example,
as further discussed in Chapter 4 and Appendix G of this
report, numerous lakes, reservoirs, rivers, estuaries (e.g.,
the Gulf of Mexico), and fjords worldwide exhibit N and
phosphorus (P) co-limitation, either simultaneously or
in seasonally-shifting patterns. Therefore, strategies are
needed to  reduce both P and N inputs, and not all control
practices will be effective for dual nutrient reduction.
Synergistic effects on nutrient loss reductions can occur
where combinations of control practices  produce more
or less than the sum of their individual reductions (U.S.
EPA SAB, 2007). An integrated strategy  should take this
into consideration.

Objectives of the SAB Integrated
Nitrogen Committee study
   The EPA Science Advisory Board formed the
Integrated Nitrogen Committee to assist EPA in its
understanding and management of nitrogen-related
air, land, and water pollution issues.  In this report, the
Committee has provided findings and recommendations
addressing the following objectives.  Assessment of
the challenges and costs to EPA of implementing the
recommendations is beyond the scope of the report.

1. Identify and analyze, from a scientific
perspective, the problems Nr presents in the
environment and the links among them.
   To address this objective, the Committee used the
nitrogen cascade framework to determine the major
sources of newly created Nr in the U.S. (Figure ES-1).
The flows of Nr within the food, fiber, feed and bioenergy
production systems and developed lands in the U.S. were
examined, paying special attention to the locations within
each of these systems where Nr is lost to the environment.
The same  process was employed for fossil fuel energy
production but, since all the Nr formed and released
during energy production is lost to the environment, the
Committee identified the important energy producing
sectors that contribute to Nr emissions.
   The Committee next examined the fate of the Nr
lost to the environment, estimated the amount stored in
different systems (e.g., forest soils) and tracked Nr as
it is transferred from one environmental system (e.g.,
the atmosphere) to another (e.g., terrestrial and aquatic
ecosystems).
   Source and fate analyses set the stage for identifying
the environmental and  human health problems Nr presents,
and the links among them. Using the nitrogen cascade, the
Committee identified the impacts Nr has on people and
ecosystem functions as it moves through each system. The
Committee also addressed the alternative metrics that could
be used, including the number of tons of specific forms of
Nr, human health indicators and the economic damage cost,
to assess incommensurable impacts due to environmental
changes (e.g., acid deposition) vs.  impacts due to losses of
ecosystem services (e.g., loss of biodiversity), and trade-
offs among Nr impacts.

2. Evaluate the contribution  an  integrated
nitrogen management strategy6 could make to
environmental protection.
   An integrated management strategy should take
into account the contributions of all Nr sources, and all
chemical species of Nr that adversely impact both human
health and environmental systems. Further, an integrated
strategy should ensure that solving one problem related
to Nr does not exacerbate another problem or diminish
ecosystem services that support societal demands. In
short, the strategy should seek to achieve desirable
benefits of Nr,  while limiting adverse effects.
6 An integrated nitrogen management strategy takes a holistic approach for managing Nr. In the context of the nitrogen cascade, all Nr anthropogenic
 creation and destruction mechanisms and all Nr uses are recognized. The strategy should take account of synergies and trade-offs, to ensure that
 decreasing one problem related to nitrogen does not result in other unintended adverse environmental, economic and societal consequences. By
 identifying relative priorities, assessing cost effectiveness and risks, the strategy should seek to maximize the benefits of Nr, while limiting overall
 adverse effects.

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   To address this challenge, the Committee identified
several actions that could be taken to better manage
Nr in one environmental system and avoid unintended
consequences in another. Examples of "integrative"
management actions that could be taken are highlighted in
that discussion.

3. Identify additional risk management options
for EPA's consideration.
   As further discussed below, the Committee has
identified four major goals for management actions that
collectively have the potential to decrease Nr losses
to the environment by about 25 percent. Decreasing
Nr emissions by these actions will result in further
decreases in Nr-related impacts throughout the nitrogen
cascade. The Committee has suggested several ways to
attain these management goals including conservation
measures, additional regulatory steps, voluntary actions,
application of modern technologies, and end-of-pipe
approaches. These are initial but significant actions;
however, others should be taken once the recommended
actions are completed and assessed, and further
opportunities are explored in an adaptive  management
approach. Thus, the last sections of this report focus on a
better understanding of Nr dynamics and  impacts in the
U.S. that could lead to more cost efficient management,
balancing human and environmental needs.

4. Make recommendations to EPA concerning
improvements in nitrogen research to support
risk reduction.
   In this report, the Committee has provided numerous
recommendations for additional Nr research to support
risk reduction activities. These research recommendations
are discussed in various chapters of the report and
are consolidated in the summary of findings and
recommendations presented in Chapter 6.

Major Findings and  Recommendations
   Throughout the report there are boxes containing
summary statements labeled "Findings." Attached to these
findings are one or more specific "Recommendations" for
actions that could be taken by  EPA or other management
authorities. In each case, the intent is to provide the
scientific foundation regarding a specific Nr-relevant
environmental issue and one or more recommendations
by which EPA acting alone or  in cooperation with other
organizations could use currently available technology to
decrease the amount of Nr lost to the U.S. environment.
The findings and recommendations are consolidated in
Chapter 6 of this report.

Overarching recommendations
   Optimizing the benefits of Nr, and minimizing its
impacts, will require an integrated nitrogen management
strategy that involves action not only on the part of EPA,
but also coordination with other federal agencies, the
states, the private sector, universities,  and the public,
supported by a strong public outreach program. Therefore
the Committee has also provided four overarching
recommendations to assist EPA in its understanding and
management of nitrogen-related air, land, and water
pollution issues:
   Overarching Recommendation 1: The Committee
recommends an integrated approach to the management
of Nr. This approach draws upon a combination of
implementation mechanisms. Each mechanism must
be appropriate to the nature of the problem at hand,
be supported by critical research on decreasing the
risks of excess Nr, and reflect an integrated policy that
recognizes the complexities and tradeoffs associated with
the nitrogen cascade. Management efforts at one point
in the cascade may be more efficient and cost effective
than control or intervention at another point. This is why
understanding the nature and dynamics of the N cascade
is critically important.
   Overarching Recommendation 2: The framing
of the reactive nitrogen cascade provides a means for
tracking nitrogen as it changes form and passes through
multiple ecosystems and media. This complexity
requires the use of innovative management systems and
regulatory structures to address the environmental and
human health implications of the most damaging  forms
and quantities of Nr. It is difficult to create de novo
fully effective regulations for such a complex system
so we recommend utilizing adaptive management to
continuously improve the effectiveness and lower the
cost of implementation policies. This in turn will  require
a monitoring system that will provide feedback on the
effectiveness of specific actions taken to lower fluxes  and
concentrations of Nr.
   Overarching Recommendation 3:  An intra-
Agency Nr management task force within EPA is
recommended to build on existing Nr research and
management capabilities within the Agency. This
task force should be aimed at increasing scientific
understanding of: (1) Nr impacts on terrestrial and
aquatic ecosystems, human health, and climate;  (2)
Nr-relevant monitoring requirements; and (3) the most
efficient and cost-effective means by which to decrease
various adverse impacts of Nr loads as they cascade
through the environment.
   Overarching Recommendation 4:  Successful Nr
management will require changes in the way EPA
interacts with other agencies. Coordinated federal
programs could better address Nr concerns and
help ensure clear responsibilities for monitoring,
modeling, researching and managing Nr in the
environment. Thus, the Committee recommends that
EPA convene an inter-agency Nr management task
force. It is recommended that the members of this
inter-agency task force include at least the following
federal agencies: U.S. Department of Agriculture,

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U.S. Department of Energy, U.S. Department of
Housing and Urban Development, U.S. Department
of Transportation, National Oceanic and Atmospheric
Administration, U.S. Geological Survey, U.S. Forest
Service, and Federal Emergency Management Agency.
The EPA Office of International and Tribal Affairs
should work closely with the Department of State to
ensure that EPA is aware of international efforts to
control Nr and is developing national strategies that
are compatible with international initiatives. Similar
recommendations for coordination and joint action
among and between agencies at both state and federal
levels have been made in the National Research
Council's recent reports on the Mississippi Basin
(NRC, 2008b, 2009). These intra- and inter-agency
Nr management task forces  should take a systems
approach to research, monitoring, and evaluation to
inform public policy related to Nr management, and
implement a systems approach to Nr management, as
recommended by the Committee.

Summary of specific recommendations
by study objective
  The Committee's findings and recommendations
corresponding to each of the four study objectives are
summarized briefly below.

1. Identify and analyze, from a scientific
perspective, the problems Nr presents in the
environment and the links among them.
  The Committee finds that uncertainty associated
with rapid expansion of biofuels, losses of Nr from
grasslands, forests, and urban areas, and the rate and
extent of denitrification have created the need to
measure, model, and report all forms of Nr consistently
and accurately. Addressing this need will decrease
uncertainty in the understanding of the fate of Nr that
is introduced into the environment and lead to a better
understanding of the impacts of excess Nr on the health
of people and ecosystems. This should be accomplished
through a coordinated effort among cognizant federal
and state agencies, and universities.
  In addition, the Committee recommends that EPA
routinely and consistently account for the presence
of Nr in the environment in forms appropriate to the
medium in which they occur (air, land, and water) and
that accounting documents be produced and published
periodically (for example, in a fashion similar to
National Atmospheric Deposition Program summary
reports). The Committee  understands that such an
undertaking will require substantial resources, and
encourages the Agency to develop and strengthen
partnerships with appropriate federal and state
agencies, and private sector organizations, with parallel
interests in advancing the necessary underlying science
of Nr creation, transport and transformation, impacts,
and management.
2. Evaluate the contribution an integrated
nitrogen management strategy could make to
environmental protection.
  The Committee finds that effective management of Nr in
the environment must recognize the existence of tradeoffs
across a number of impact categories involving the cycling
of nitrogen and other elements. In addition, an integrated
multi-media approach to monitoring Nr is needed.
  In that regard, the Committee recommends that:
1. EPA should develop a uniform assessment and
  management framework that considers the effects of
  Nr loading over a range of scales reflecting ecosystem,
  watershed, and regional levels. The framework should
  include all inputs related to atmospheric and riverine
  delivery of Nr to estuaries, their comprehensive effects
  on marine eutrophication dynamics, and their potential
  for management.
2. EPA should examine the full range of traditional and
  ecosystem response categories, including economic and
  ecosystem services, as a basis for expressing Nr impacts
  in the environment, and for building better understanding
  and support for integrated management efforts.
3. Identify additional risk management options
for EPA's consideration.
  The Committee finds that a number of risk
management actions should be considered to reduce Nr
loading and transfer to the environment. These include
farm-level improvements in manure management, actions
to reduce atmospheric emissions of Nr, and interventions
to control Nr in water management programs. As an
example, the Committee recommends that EPA should
reexamine the criteria pollutant "oxides of nitrogen" and
the indicator species NO2 and consider supplementing this
with NHX and NOy as indicators of chemically reactive
nitrogen (Nr without ^O).
4. Make recommendations to EPA concerning
improvements in nitrogen research to support
risk reduction.
  The Committee finds that research is needed in a
number of areas to support Nr risk reduction activities.
These areas include research to advance the understanding
of: the quantity and fate of Nr applied to major crops;
how to accelerate crop yields while increasing N fertilizer
uptake efficiency; agricultural emissions of forms of
Nr;  atmospheric deposition of Nr; and the potential for
amplification of Nr-related climate impacts.

Four recommended management
actions
  Consistent with the overarching and specific
recommendations noted above, the Committee identified
four management actions that could be undertaken in
the near term by applying existing proven science and
technology and determined how those actions could
contribute to the reduction of excess Nr in the environment.

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1. The Committee estimates that if EPA were to expand
  its NOX control efforts for emissions of mobile sources
  and power plants, a 2.0 Tg N/yr decrease in the
  generation of reactive nitrogen could be achieved. Such
  changes can be effected by applying existing, proven
  technology. Emissions from many point sources are
  controlled with low-NOx burners or NOX reduction.
  Such equipment should also be installed on industrial
  boilers and the remaining, uncontrolled power plants.
  NOX controls for modern on-road vehicles are effective
  and these technologies should be applied to off-road
  vehicles, locomotives, ships and other devices with
  internal combustion engines.
2. The Committee estimates that excess flows of Nr into
  streams, rivers, and coastal systems can be decreased
  by approximately 20% (approximately 1 Tg N/yr)
  through improved landscape management and without
  undue disruption to agricultural production. This would
  include activities such as using large-scale wetland
  creation and restoration to provide needed ecosystem
  services of Nr retention and  conversion as well as
  matching cropping systems and intensity of Nr use to
  land characteristics. Improved tile-drainage systems
  and riparian buffers on cropland, and implementing
  stormwater and non-point source management practices
  (e.g., EPA permitting and funding programs) are
  important components. In addition, the Committee
  estimates that crop N-uptake efficiencies can be
  increased by up to 25% over current practices through
  a combination of knowledge-based practices and
  advances in fertilizer technology (such as controlled
  release and inhibition of nitrification). Crop output can
  be increased while decreasing total Nr by up to 20% of
  applied artificial Nr, amounting to -2.4 Tg N/yr below
  current amounts of Nr additions to the environment.
  These are appropriate actions that could be taken with
  today's available technologies and further progress is
  possible.
3. The Committee estimates that livestock-derived NH3
  emissions can be decreased by 30% (a decrease of 0.5
  Tg N/yr) by a combination of BMPs and engineered
  solutions. This is expected to decrease PM2 5 by
  approximately 0.3 micrograms per cubic meter (2.5%),
  and improve health of ecosystems by achieving
  progress towards critical load recommendations.
  Additionally we estimate that NH3 emissions derived
  from fertilizer applications can be decreased by
  20% (decrease by approximately 0.2 Tg N/yr),
  through BMPs that focus on improvements related to
  application rate, timing, and placement.
4. The Committee recommends that a high priority be
  assigned to increasing funding for nutrient management.
  We estimate that adequate financial support for sewage
  treatment infrastructure upgrades to remove nutrients
  could decrease Nr emissions by between 0.5 and 0.8
  Tg N/yr. Additional Nr management from eligible
  stormwater and nonpoint sources could be accomplished
  through increased support.
   Implementing these suggestions will decrease the
amount of Nr introduced into the United States by about
25%, which will similarly decrease the amount of Nr
lost to the atmosphere, soils and waters. The Committee
believes that these represent realistic and attainable
near-term outcomes, however further reductions are
undoubtedly needed for many N-sensitive ecosystems and
to ensure that health-related standards are maintained.

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Reactive Nitrogen
in the United States
AN ANALYSIS OF INPUTS, FLOWS, CONSEQUENCES,
AND MANAGEMENT OPTIONS

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 1
 Introduction
 1.1. Overview of the Problem - Impacts
 of Excess Nr on Human Health and the
 Environment
   Nitrogen is an essential nutrient that governs the
 growth and reproduction of living organisms. Reactive
 nitrogen (Nr), in contrast to non-reactive gaseous N2,
 includes all biologically active, chemically reactive,
 and radiatively active nitrogen (N) compounds in the
 atmosphere and biosphere of the Earth. Anthropogenic
 creation of Nr provides essential benefits for humans -
 first and foremost in meeting human dietary needs. In fact,
 a large proportion of the human population of the earth
 could not be sustained if synthetic nitrogen fertilizers did
 not augment food production significantly all over the
 world.  However, excess releases of Nr to the environment
 from human activities such as fossil fuel combustion and
 agriculture are a major cause of air and water quality
 degradation that has been linked to significant impacts on
 human and ecosystem health.
 Reactive nitrogen (Nr) includes inorganic chemically
 reduced forms of N (NHx) [e.g., ammonia (NH^) and
 ammonium ion (NH^+JJ, inorganic chemically oxidized
forms of N [e.g., nitrogen oxides (NOy),  nitric acid
 (HNO^), nitrous oxide fTV^Oj, A^Oj, HONO, peroxy
 acetyl compounds such as peroxyacytyl nitrate (PAN),
 and nitrate ion (NO'$-)], as well as organic compounds
 (e.g., urea, amines,  amino acids, and proteins).
   The negative consequences of Nr flux in the U.S.
 environment include increases in photochemical smog
 and atmospheric paniculate matter (PM2 5), decreases
 in atmospheric visibility, both increases and decrease
 in productivity of grasslands and forests, acidification
 of soils and freshwaters, accelerating estuarine and
 coastal eutrophication, increases in the emission of
 greenhouse gases (GHG) to the atmosphere, and decreases
 in stratospheric ozone concentrations.  Most of these
 changes in environmental conditions lead to a variety of
 negative impacts on both ecosystem and human health
 (Johnson and Siccama, 1983; Heck et  al., 1984; Paerl,
 1988; MacKenzie andEl-Ashry, 1990; ECOHAB, 1995;
 Bricker et al., 1999; Rabalais et al., 1999; NRC, 2000;
 Mitsch et al., 2001; Fenn et al., 2003; Ezzati et al.,
 2004; Mokad et al., 2004; Verity et al., 2006; U.S. EPA
 Clean Air  Scientific Advisory Committee, 2008; U.S.
 EPA SAB, 2008; Bobbink et al., 2010). In light of the
 magnitude of the human alteration of the nitrogen cycle,
 and the resulting negative consequences on humans
 and ecosystems, the National Academy of Engineering
 has identified management of the nitrogen as one of the
"grand challenges" facing this country (National Academy
of Engineering, 2008).

1.2. The Nitrogen Cascade - Nr Loading,
Cycling, and Exposure
  Approximately 78% of the atmosphere is diatomic
nitrogen (N2), which is unavailable to most organisms
because of the strength of the triple bond that holds the two
N atoms together. Over evolutionary history, only a limited
number of species of bacteria and archaea have evolved
the ability to convert N2 to Nr via biological N fixation.
Thus, even with adaptations to use N efficiently, many
ecosystems of the world are limited by N.

Anthropogenic creation ofNr
  Nitrogen limitation of ecosystem production has
driven humans to use increasingly sophisticated and
energy-intensive measures to obtain Nr to sustain food
production and to produce  other commodities (e.g.,
nylon, explosives). In pre-history, hunters and gatherers
harvested food from natural stocks.  With the advent of
agriculture, local sources of Nr were used (soil stocks,
crop residue, and manures) to  increase productivity
of landscapes. In the nineteenth century, long-range
transport of Nr to sustain food production increased
with the shipment of bird guano from the Pacific Islands
and nitrates from South America to Europe and other
locations. By the beginning of the twentieth century, these
sources were not sufficient to sustain the growing global
population requirements for food.
  This deficiency led to what has been called one of
the world's most important discoveries - how to extract
N2 from the atmosphere and convert it to ammonia
(NH3) - called the Haber-Bosch process (Smil, 2001;
Erisman et al., 2008). Today, this process and cultivation-
induced biological N fixation (C-BNF) introduce over
140 teragrams (Tg) of N per year (hereafter expressed
as Tg N/yr) into the global environment to increase food
production (Galloway et al., 2008). Another 23 Tg N/
yr are introduced by the Haber-Bosch process for the
chemical industry, and 25 Tg N/yr are introduced via the
combustion of fossil fuels (Galloway et al., 2008).
  The total global anthropogenic Nr creation rate is -190
Tg N/yr (2005), substantially larger than the median of
estimates for Nr creation by natural  terrestrial processes
(-100 Tg N/yr) (Galloway et al., 2008). The fact that
humans are more effective than nature in Nr creation
means that on average, humans are less reliant on natural
sources of Nr. However, with global commodity stocks
running at a 58-day supply and food prices increasing

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dramatically, the challenge is to increase the nutrient use
efficiency of Nr in agricultural systems while maintaining
or increasing yields (USDA, ERS/World Agricultural
Outlook Board, July 11, 2008. World Agricultural Supply
and Demand Estimates).
   There are large regional disparities in Nr creation rates
on both absolute and per capita bases. Total Nr creation
is larger in Asia than in any other region. Per capita Nr
creation is largest in North America and Europe. Humans
also redistribute large amounts of Nr among countries
or regions of the world through exports of fertilizers,
feed grains,  and fossil fuels. Nevertheless, there are large
regions of the world with populations approaching one
billion, where there is malnutrition in part due to a lack of
available Nr to  sustain crop production.
   The introduction of Nr into most regions of the United
States by humans has greatly increased food availability.
However, since essentially all the Nr created for food
production and by fossil fuel combustion is lost to the
environment, it has also greatly increased the contribution
of Nr to a wide variety of environmental problems.
Most plants, animals, and microorganisms are adapted
to efficiently use and retain Nr. Addition of Nr to most
ecosystems may first lead to increased uptake, growth,
and storage - and hence to increased biomass, including
food or fiber production. However, further addition of
Nr in excessive amounts often leads to imbalances in the
movement of Nr among reservoirs and potential losses7
to the environment in the form of air emission or water
discharges into other ecosystems where Nr may disrupt
ecosystem functions and have a negative impact on
resources. In essence, the assimilative capacity of the
ecosystem may be insufficient to benefit from increases in
Nr without disruptive changes.
   These changes, which impact air, land, water and the
balance of life in an interrelated fashion, are often referred
to as a cascade of effects from excess Nr8 or the "nitrogen
cascade" (Figure 1). Unlike other element-based pollution
problems, the N cascade links the negative impacts,  where
one N-containing molecule can in sequence contribute to
all the  environmental issues mentioned above.
   The nitrogen cascade has three dimensions:
biogeochemical, alterations in the environment, and
human and ecosystem consequences.
   The "biogeochemical" dimension of the nitrogen
cascade involves: Nr creation from N2 as a consequence
of chemical, food and energy production; Nr use in food
and chemical production; Nr losses to the environment;
changes in Nr species residence times in environmental
reservoirs; Nr transfers among reservoirs; and Nr
conversion back to N2. Alterations to the environment
then result from increased Nr levels in the environment.
These alterations have negative consequences for
ecosystem and human health at local, regional, national
and global scales. Because nitrogen is a critical resource
and also a contributor to many of the environmental
concerns facing the U.S. today, it is imperative to
understand how human action has altered N cycling in the
U.S., and the consequences of those alterations on people
and ecosystems. The overarching question is, how do
we protect and sustain ecosystems that provide multiple
benefits to society while also providing the  interconnected
material, food and energy  required by society?
   Nr inputs to the nation  and the world have been
increasing, largely due to human activities associated
with food production and fossil fuel combustion. Despite
the obvious benefits of a plentiful supply of food and
energy, the adverse  consequences associated with the
accumulation of Nr in the  environment are large, with
implications for human health and the environment.
   The greater the inputs of Nr to the landscape, the
greater the potential for negative effects, caused by
greenhouse gas (GHG) production, ground  level ozone,
acid deposition,  and Nr overload that can contribute
to climate change, degradation of soils and vegetation,
acidification of streams, lakes and rivers, estuarine and
coastal eutrophication, hypoxia and habitat loss.
   The growing nature of the Nr problem, and the adverse
and intertwined consequences associated with Nr inputs
to air, land, and water as exhibited in the N cascade
underscore the need for researchers and managers to
explore integrated strategies that minimize N inputs,
maximize its use efficiency, promote Nr removal
processes and protect humans and natural resources.
   The concept of the nitrogen cascade highlights that
once a new Nr molecule is created, it can, in sequence,
travel throughout the environment contributing to major
environmental problems (Galloway et al., 2003). The
adaptation of the cascade in Figure 1 was developed by
the SAB Integrated Nitrogen Committee (INC) to provide
a context for considering nitrogen-related issues and
ecosystem effects in the U.S. To consider the cascading
effects of Nr in the U.S., we examined the various
atmospheric, terrestrial, and aquatic environmental
  In the context of this report, "losses" refers to transfers among systems and not the conversion of Nr to N2- Whenever N2 formation is discussed, it is
  explicitly stated.

  Excess reactive nitrogen (Nr) is denned as the amount of Nr that is present in, or introduced into, an environmental system (e.g., Nr inputs to the
  atmosphere, Nr inputs to grasslands and forests, Nr inputs to estuaries) from anthropogenic sources that is not incorporated into agricultural and
  other biological products (e.g., food, feed, fuel, and fiber), or stored in long-term storage pools (e.g., cropland soils).Thresholds are used to determine
  the amount of excess Nrthat causes negative effects on ecosystem services and functions, and human health. Thresholds vary by metric (e.g.,
  concentration, loading, etc) and depend on the environmental system (e.g., atmosphere, forest). Examples for specific thresholds are given later in the
  report in relevant sections.

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The  Nitrogen Cascade
        New Nitrogen
        Energy Production     NOX
        & combustion of fossil fuels
        Food Production       ™
        & creation of synthetic fertilizers
                            N0y, NHX, Norg
Atmospheric
                        NOy, NHX, Norg
                                                Terrestrial
                                                     Agricultural
                                                   crops
                                                              animals
  t-J
                                                         soils
                               Vegetated
forests     grasslands
            u
                                                                                  s°iis
                               Populated
people I-M landscape
               i
                                                                                  soils
                        N0y, NHX, Norg
Figure 1: The nitrogen cascade
                                                    NOX
                                                    NH3
                                                    Norg
                                                                                                     N20
systems where Nr is stored, and the magnitudes of
the various flows of N to, from, and within them. The
nitrogen cascade concept implies the cycling of Nr among
these systems. The process of denitrification is the only
mechanism by which Nr is converted to chemically
inert N2, "closing" the continuous cycle (Figure 1 shows
only flows of reactive nitrogen, not N2). Denitrification
can occur in any of the indicated reservoirs except the
atmosphere.
   The "new" N box in Figure 1 depicts the two primary
anthropogenic sources by which Nr originates - energy
production and food production - and where Nr from
these sources enter ecosystems. Energy production
includes both fossil fuel and biofuel combustion. Food
production includes N fertilizer produced in the U.S..
cultivation-induced biological N (C-BNF) in the U.S..
production of animals and crops in the U.S. for human
consumption, and imports of N-containing fertilizer, grain
and meat to the U.S.
   The atmospheric system box in Figure 1 indicates that
         tropospheric concentrations of both ozone and paniculate
         matter are increased due to emissions of nitrogen oxides9
         (NOX) to the atmosphere. The ovals illustrate that the
         increase in N2O concentrations, in turn, contribute to
         the greenhouse effect in the troposphere and to ozone
         depletion in the stratosphere. Except for N2O, there is
         limited Nr storage in the atmosphere. Losses of Nr from
         the atmospheric system include total oxidized nitrogen10
         (NOy), reduced nitrogen11 (NHX), and organic nitrogen
         (Norg) deposition to terrestrial and aquatic ecosystems of
         the earth's surface. There is little potential for conversion
         of Nr to N2 via denitrification in air. However, once
         airborne deposition of Nr occurs it will be subject to
         denitrification pathways via soil and water.
            The terrestrial system box in Figure 1 depicts that
         Nr enters agricultural lands via food production and
         is introduced to the entire terrestrial landscape via
         atmospheric deposition. Within agricultural regions there
         is cycling among soils, crops and animals, and then a
         transfer of Nr as food to populated regions, from which
         there are Nr losses to the environment (e.g., sewage.
9 NOX (oxides of nitrogen) includes NO + NO2

10 NOy (total oxidized nitrogen) includes NO, NO2, NO3, N2O5, HONO, HNO3, NO3% PAN and other organo-nitrates, RONO2

11 NHX (reduced nitrogen) includes NH3 + NH4

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landfills). The ovals showing ecosystem productivity
and biogeochemical cycling reflect that Nr is actively
transported and transformed within the terrestrial system,
and that as a consequence there are significant impacts
on ecosystem productivity due to fertilization and
acidification, often with resulting losses of biodiversity.
There is ample opportunity forNr storage inbothbiomass
and soils. Losses of Nr from this system occur by leaching
and runoff of NOy, NHX and Norg to aquatic ecosystems
and by emissions to the atmospheric system as NOX, NH3,
Norg, and N2O. There is potential for conversion of Nr to
N2 via denitrification in the terrestrial system.
   The aquatic system box in Figure 1 shows that Nr
is introduced via leaching and runoff from terrestrial
ecosystems and via deposition from atmospheric
ecosystems.  Connected with the hydrological  cycle, there
are Nr fluxes downstream with ultimate transport to coastal
systems. Within the aquatic system, the ovals highlight
two significant impacts of waterborne Nr acidification of
freshwaters and eutrophication of fresh and coastal waters.
Except for Nr accumulation in groundwater reservoirs,
there is limited Nr storage within the hydrosphere. Losses
of Nr from the aquatic system are primarily via N2O
emissions to  the atmospheric system. There is a very large
potential for  conversion of Nr to N2 via denitrification in
water and wetlands.
   NOy, NHX and N2O are all components of Nr, but
a fundamental difference is that the NOy and NHX are
rapidly transferred from the atmosphere to receiving
ecosystems due to a short atmospheric residence time
(< 10 days) where they continue to contribute to the N
cascade (Galloway et al., 2004). Because of its  longer
residence time (-100 years) however, N2O remains in the
troposphere where it contributes to climate change, until
it is transferred to the stratosphere, where it contributes to
ozone depletion (Galloway et al., 2004).

1.3. EPA Activities to Manage Risks
Posed by Nr
   EPA activities to manage the risks posed by reactive
nitrogen can be linked to the Agency's broad  strategic
goals. EPA's mission is to protect human health and
the environment. In achieving this mission, EPA is
accountable for addressing five goals given in the 2006 -
2011 EPA Strategic Plan (U.S. EPA, 2006d):
1. Clean air and global climate change
2. Clean and safe water
3. Land preservation and restoration
4. Healthy communities and ecosystems
5. Compliance and environmental stewardship
   The Strategic Plan includes targets for reducing risk
from N. EPA's Report on the Environment (U.S. EPA,
2008c), provides "data on environmental trends," to
determine whether or not EPA is on track to meet its
targets and goals. EPA is responsible and accountable for
reducing at least some risks from Nr.
   As previously discussed, the principal mechanisms
for Nr removal from circulation in the environment are
complete denitrification (re-conversion of Nr back to non-
reactive gaseous N2), and storage in long-term reservoirs
(e.g., soils, sediments, and woody biomass). In some cases,
it may be possible to capture Nr emissions or discharges
and deliver them to food or fiber production areas where
there are nitrogen deficiencies. However, as previously
noted, major challenges in the management of the N cycle
are how to decrease creation of Nr while still meeting
societal needs, promote denitrification of excess Nr
(without producing N2O), and improve the efficiency of use
and reuse of excess Nr in a cost-effective manner. Solving
these challenges will result in less Nr accumulation.
   The parts of EPA most directly concerned with
managing or conducting research on Nr are the Office  of
Air and Radiation, the Office of Water, and the Office of
Research and Development. Programs designed to save
energy, such as Energy Star, tend to reduce emissions of
Nr as well. In over a dozen programs, EPA's Office of Air
and Radiation reduces risks from Nr. These programs and
related activities include:
   National Ambient Air Quality Standards (NAAQS)
   standard setting and implementation;
   Emission standards for industrial stationary sources and
   area sources
   Acid Rain Program
   Clean Air Interstate Rule
   Programs that focus on mobile source emissions
   EPA's Office of Water addresses Nr under both the
Clean Water Act and the  Safe Drinking Water Act through
activities such as:
   Criteria development and standard setting
   Total maximum daily load (TMDL) development
   National Pollution Discharge Elimination System
   (NPDES) permits
   Watershed planning
   Wetlands preservation
   Regulation of concentrated animal feeding operations
   (CAFOs).
   EPA's Office of Research and Development aims to
conduct leading-edge research and foster the sound use of
science and technology in support of EPA's mission. The
Office of Research and Development is well recognized
for providing a scientific basis  for the development of
the National Ambient Air Quality Standards for NOX
and paniculate matter (PM). The Office of Research and
Development's Ecosystem Services Research Program
has been developed to identify and quantify the positive

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and negative impacts on ecosystem services resulting
from changes in nitrogen loadings from major source
categories to support policy and management decisions in
EPA's Offices of Air and Radiation and Water.
   EPA has brought a great variety of risk reduction tools
to bear on reactive N:
  Conventional regulation and enforcement
  Cap and trade approaches
  Measurement, monitoring and place-based approaches
  Control technology development and verification
  Communication and education
  Intergovernmental and international cooperation
  Voluntary approaches
   The variety and breadth of EPA programs addressing
Nr reflect the ubiquity of Nr in the environment, the
historical single medium regulatory approach, and the
lack of a silver bullet for reducing risks from Nr.

Need for an Integrated Nitrogen
Management Strategy
   The EPA programs discussed above (and the programs
of EPA's predecessor organizations) have been active in
the management of Nr through efforts to: decrease the Nr
amount in sewage, control NOX to decrease photochemical
smog and acid rain, control Nr inputs to coastal
systems, control fine particulates in the atmosphere, and
decrease Nr leaching and runoff from crop and animal
production systems. As beneficial as those efforts have
been, they have focused on the specific problem without
consideration of the interaction of their particular system
with other systems downstream or downwind. Given the
reality of the nitrogen cascade, this approach may result
in short-term benefits for a particular system but will also
likely only temporarily delay larger-scale impacts on other
systems. Thus, there is a need to integrate N management
programs, to ensure that efforts to lessen the problems
caused by N in one area of the environment do not result
in unintended problems in other areas.
   Biofuels feedstock production is a good example
of this. Increasing corn production for ethanol raised
the prospect of increased Nr losses and degraded water
quality. The alternative of cellulosic based ethanol does
not necessarily mitigate the potential for this negative
externality. High yields of cellulosic materials also require
N and the "marginal" land assumed for such production
may be more susceptible to nutrient leakage (NRC, 2008a).
   In addition, there can be unintended consequences
associated with a focus on one pollutant, even an integrated
focus on various forms of nitrogen. For example, as further
discussed in Chapter 4 and Appendix G of this report,
numerous lakes, reservoirs, rivers, estuaries (e.g., the Gulf
of Mexico), and fjords worldwide exhibit N and P co-
limitation, either simultaneously or in seasonally-shifting
patterns. Therefore, strategies are needed to reduce both P
and N inputs, and not all control practices will be effective
for dual nutrient reduction. There can be synergistic
effects on nutrient loss reductions where combinations of
control practices can produce more or less than the sum of
their individual reductions (U.S. EPA SAB, 2007) and an
integrated strategy should take this into consideration.

1.4. SAB Integrated  Nitrogen  Committee
Study Objectives
  The EPA Science Advisory Board has previously
provided advice concerning management of nitrogenous
compounds as well as integrated environmental decision
making. In 1973, the Science Advisory Board issued
a report Nitrogenous Compounds in the Environment
(U.S. EPA SAB, 1973). The report addressed sources and
effects of nitrogenous compounds, including those from
air emissions, animal wastes, crop agriculture, industrial
processes, and solid wastes. The SAB concluded that,
"At present, all known trends appear to be ones that
can be managed and kept within control, if appropriate
steps are taken now," and provided recommendations
relating to Nr research and control. In its 2000 report,
Toward Integrated Environmental Decision-Making (U.S.
EPA SAB, 2000) the SAB articulated a framework for
integrated environmental decision-making. In that report,
the  SAB noted that the three-phased structure of the
framework (problem formulation, analysis and decision-
making, followed by implementation and evaluation)
"belies the complexities involved in putting the concept
of integrated decision-making into practice." The SAB's
interests in N science and integrated environmental
protection converged in 2007, when the SAB identified
integrated N research and control strategies as an
important issue facing the Agency and formed the
Integrated Nitrogen Committee (the Committee) to
conduct this study.
  The Committee was charged by the Science Advisory
Board to address the following four objectives:
1. Identify and analyze, from a scientific perspective, the
  problems reactive nitrogen presents in the environment
  and the links among them;
2. Evaluate the contribution an integrated nitrogen
  management strategy12 could make to environmental
  protection;
12 An integrated nitrogen management strategy takes a holistic approach for managing Nr. In the context of the nitrogen cascade, all Nr anthropogenic
  creation and destruction mechanisms and all Nr uses are recognized. The strategy should take account of synergies and trade-offs, to ensure that
  decreasing one problem related to nitrogen does not result in other unintended adverse environmental, economic and societal consequences. By
  identifying relative priorities, assessing cost-effectiveness and risks, the strategy should seek to maximize the benefits of Nr, while limiting overall
  adverse effects.

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3. Identify additional risk management options for EPA's
  consideration; and
4. Make recommendations to EPA concerning
  improvements in nitrogen research to support
  risk reduction.
   In this report the Committee has provided findings
and recommendations addressing the study objectives.
We recognize that there will be challenges and costs
associated with the pursuit of the recommended
management strategies, and that EPA requires
statutory authority to take regulatory action. However,
assessment of the challenges and costs to the Agency of
implementing the recommendations is beyond the scope
of this report.

1.5. Study Approach and Structure of
the Report
   To address the four objectives of this study, the
Committee completed the following activities:
1. The Committee used the  nitrogen cascade framework
  to determine the major sources of newly created Nr
  in the U.S. The flows of Nr within the food, fiber,
  feed, andbioenergy production systems of the U.S.
  were examined, paying special attention to the
  locations in each of these systems where Nr is lost
  to the environment. The same process was employed
  for energy production but, since all of the Nr formed
  during energy production is lost to the environment, the
  Committee identified the important energy producing
  sectors that contribute to  Nr formation.
2. The Committee examined the fate of Nr lost to the
  environment, estimated the amount stored in different
  systems (e.g., forest  soils), and tracked Nr as it is
  transferred from one environmental system (e.g., the
  atmosphere) to another (e.g., terrestrial and aquatic
  ecosystems).
3. Using the nitrogen cascade, the Committee identified
  the impacts Nr has on people and ecosystem functions
  as it moves through different systems.
4. The Committee identified actions that could be
  taken based on available  science and management
  practices to improve the integrative management of
  N. The Committee suggested ways in which each of
  these actions could be accomplished and estimated
  that together they could decrease Nr losses to the
  environment by about 25%.
5. The Committee identified research needed to improve
  the scientific foundation to support specific Nr risk
  reduction activities.
   Four public meetings were held during the course
of the study and briefings were presented to the
Committee by: EPA's Office of Air and Radiation, Office
of International Affairs, and Office of Water; the U.S.
Department of Agriculture's Agricultural Research
Service, Cooperative State Research, Extension and
Education Service, and the Economic Research Service;
and external organizations such as the Energy Research
Centre of the Netherlands, Environmental Defense
Fund, International Plant Nutrition Institute, Iowa
State University, LiveFuels, and the  Soil and Water
Conservation Society.
   Additionally, the Committee invited scientists and
managers from EPA, other federal agencies, states and
localities, academia, non-governmental organizations, and
the private sector to participate in an October 20-22, 2008
workshop and meeting on Nitrogen Risk Management
Integration. The purpose of the workshop was to receive
public input on several subjects: the  Committee's
preliminary assessment of Nr problems, consequences,
and remedies, with emphasis on risk reduction; the
Committee's quantitative estimates of attainable Nr
reductions; and mechanisms whereby the Nr strategy
might be enacted. The Committee took this public input
into consideration as it developed this report.

Structure of the report
   This report contains six chapters.  The report was
developed for a multifaceted audience of scientists and
policy makers and therefore the level of detail varies
in different sections of the document. The introductory
chapter provides an overview of problems caused
by excess reactive nitrogen and describes the study
objectives and approach. Chapters 2-6 discuss how the
Committee has addressed the four study objectives and
present specific findings and recommendations. The
findings and recommendations corresponding to each of
the study objectives are consolidated in Chapter 6.
   Study objective 1 (identification and analysis of the
  problems nitrogen presents in the  environment and
  linkages among these problems) is addressed in
   Chapters 2 and 3. Chapter 2 focuses on the sources,
  transfer, and transformation of reactive nitrogen in
  environmental systems. Chapter 3 describes the impacts
  of reactive nitrogen on aquatic, atmospheric, and
  terrestrial ecosystems.
   Study objective 2 (evaluation of the contribution an
  integrated nitrogen management strategy could make
  to environmental protection) is addressed in Chapters
  4 and 5. Chapter 4 reviews the implications for risk
  reduction  strategies for reactive nitrogen.  Chapter 5
  discusses integrated risk reduction strategies.
   Study objective 3 (identification of additional risk
  management options for EPA's consideration) is
  addressed in Chapters 5 and 6. In  Chapter 6, the
   Committee identifies specific management goals
  for reducing the loss of reactive nitrogen to the
  environment. The Committee believes that these
  represent realistic near-term management goals that
  can be attained using current technology. However, the
   Committee emphasizes that further reduction beyond

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  these goals will be needed to protect many N-sensitive
  ecosystems and to ensure that health-related standards
  are maintained.
  The Committee finds that the management goals could
  be attained by conservation measures, additional
  regulation, and application of modern technologies.
  Study objective 4 (recommendation of improvements
  in reactive nitrogen research to support risk
  reduction) is addressed in all of the report chapters
  and Chapter 6 contains a section describing the need
  for a comprehensive program to monitor Nr in the
  environment.
   Throughout this report there are boxes
containing summary statements labeled "Findings."
Attached to these findings are one or more specific
"Recommendations" for actions that could be taken by
EPA or other management authorities.

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Sources, Transfer,  and  Transformation  of
Nr  in  Environmental  Systems
   The Committee was charged with identifying and
analyzing, from a scientific perspective, the problems Nr
presents in the environment and the links among them.
This chapter addresses two aspects of the Committee's
work. The first aspect is the introduction of Nr into U.S.
environmental systems from fossil fuel combustion
and from food production, and the second aspect is the
fate of Nr after it is emitted to the atmosphere by fossil
fuel combustion or lost to the air, water and soils from
agricultural production systems. The Nr budgets and
calculations for the U.S. exclude Alaska and Hawaii
because data were not available for these areas. Most of
the Nr introduced into the environment comes from the
Haber-Boesch process. Haber-Boesch contributes three to
four times the Nr introduced from fossil fuel combustion
and most of the Haber-Boesch introduction of Nr comes
through agriculture.

2.1. Nr Flux in the Environment
   As stated previously, although N is a major required
nutrient that governs growth and reproduction of living
organisms, Nr losses to the environment from human
sources have a profound effect on air, water and soil
quality. Human consumption of energy to sustain
economic development results in emissions of NOX to
the atmosphere via fossil fuel combustion. Consumption
of food to meet nutritional requirements of a growing
population results in agricultural emissions of NH3, urban
and industrial emissions of NOX and N2O, and losses
of NO3~ and other N compounds to water bodies due to
leaching and runoff. Once released into the atmosphere by
either human or natural processes, these Nr compounds
undergo transformation through atmospheric reactions
(e.g., gas-to-particle conversion), transport associated
with wind, and finally wet and dry deposition. Reactive
nitrogen lost from agricultural and peopled systems can
enter groundwater, streams, lakes, estuaries, and coastal
waters where the Nr can also undergo transformation
mediated by a wide range of biotic and abiotic processes.
The introduction of Nr into agroecosystems provides much
of the world's food. The losses of Nr to the environment
throughout the food production process and during
fossil fuel combustion contribute to many of the major
environmental problems of today. The impacts of Nr on
humans and ecosystems are discussed in the Executive
Summary and Chapter 3 of this report.
  Some key issues concerning management of Nr in
the U.S. environment are the introduction of new Nr
by imports, fertilizer production, cultivation-induced
biological nitrogen fixation (C-BNF), and fossil fuel
combustion. Other important issues are the distribution of
Nr within agricultural systems and populated systems and
redistribution of Nr through losses from those systems to
the environment (Figure  1). National-level values for Nr
fluxes are displayed in Table 1. Fluxes that represent the
introduction of new Nr into the U.S. are marked with an
asterisk and illustrated in Figure  1. In specific sections of
this report these values have been used to more clearly
determine the flux and fate of Nr in the U.S. Figure 2
Illustrates sources of Nr introduced into the United States.
                                      Natural
                                                        Industry
                     Fossil Fuel

                               Stationary
                                                             Haber Bosch N Fertilizer
                                  Cultivation
                                               Agriculture
Figure 2: Sources of reactive nitrogen (Nr) introduced into the United States in 2002 (Tg N/yr).

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                           Table 1: Nr fluxes for the United States, Tg N in 2002.a
                            fiiizviif3iPm»Ti Ti irai i r.i t^Tfei tai IB
             Agriculture - livestock (manure) N2O-N
                                                                                      0.03
             Agriculture - soil management N2O-N
                                                                                       0.5
             Agriculture - field burning agricultural residues
                                                                                      0.001
             Fossil fuel combustion - transportation*
                                                                                       0.1
             Miscellaneous
   NHv-N emissions2
                                                                                       0.1
             Agriculture: livestock
                                                                                       1.6
             Agriculture: fertilizer
                                                                                       0.9
             Agriculture: other NHs-
                                                                                       0.1
             Fossil fuel combustion - transportation *
                                                                                       0.2
             Fossil fuel combustion - utility & industry '
                                                                                      0.03
             Other combustion
                                                                                       0.2
             Miscellaneous
   NOv-N emissions2
             Biogenic from soils
                                                                                       0.1
                                                                                       0.3
             Fossil fuel combustion - transportation *
                                                                                       3.5
             Fossil fuel combustion - utility & industry '
                                                                                       1.9
             Other combustion
                                                                                       0.4
             Miscellaneous
                                                                                       0.2
      Total Atmospheric inputs
                                                                                      10.0
                                                                                                        100
  Nr inputs to the Terrestrial environmental system
  Atmospheric N deposition13
             Organic N3
2.1
             Inorganic NOy-N4
2.7
             lnorganic-NHx-N4
   N fixation in cultivated croplands '
             Soybeans*
2.1
                                                                                       3.3
             Alfalfa*
                                                                                       2.1
             Other leguminous hay *
                                                                                       1.8
             Pasture*
                                                                                       0.5
             Dry beans, peas, lentils '
       -tion in non-cultivated
       lort in commoditi-
  Synthetic N *
             Fertilizer use on farms & non-farms
                                                                                       0.1
                                                                                      10.9
             Non-fertilizer uses
   Manure N prod-
   Human wast
                                                                                       4.2
             Total Terrestrial inputs
                                                                                      43.5
                                                                                                        100
  Nr inputs to the Aquatic environmental syst
  Surface water N flu
Terms with an asterisk indicate Nrthat is created, highlighting where reactive nitrogen is introduced to the environment.

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Table 1 Notes
   a. The Nr estimates in this table are shown with two
significant digits or 0.1 million metric tons N per year
(or Tg N/yr) to reflect their uncertainty; occasionally this
report will show data to more significant digits, strictly
for numerical accuracy. Because numbers are rounded,
the sums of source category inputs do not always equal
the total reported inputs. Obtaining quantitative estimates
of each of the Nr terms and the associated uncertainties
remains a major scientific challenge.
   b. Reducing the uncertainty in total deposition of
atmospheric Nr to the surface of the 48 contiguous United
States remains a scientific and policy priority. Based on
observations and models, we estimate 5.9 (range 4-9)
Tg N/yr total anthropogenic Nr deposition to the entire
48 States. The EPA sponsored Community Multiscale Air
Quality (CMAQ) Model run yielded a value of 4.8 Tg N/
yr. The value shown for the total (6.9 Tg N/yr) reflects the
assumption that organo-nitrogen species should be added
to the model estimate as 30% of the total.
   c. The synthetic N total includes 5.8 Tg N of fertilizer
net imports to the United States (8.25 Tg N imported -
2.41 Tg N exported) plus 9.4 Tg N of fertilizer produced
in the United States in 2002.
   Table 1 Data Sources (all data reflect N fluxes in the
United States in 2002)
   1 Emissions, N2O-N (U.S.  EPA Inventory of U.S.
Greenhouse Gas Emissions and Sinks, 1990-2006)
   2 Emissions, NHX-N; Emissions, NOX-N (U.S. EPA
National Emissions Inventory, release version October
2007)
   3 Atmospheric deposition, organic N (30% of total
atmospheric N deposition, Neff et al. 2002)
   4 Atmospheric deposition, inorganic NOy-N and
NHX-N (U.S. EPA CMAQ model)
   5 N2 fixation in cultivated croplands (USDA census of
agriculture 2002, literature coefficients)
   6 N2 fixation in non-cultivated vegetation (unpublished
data estimate after Cleveland and Asner, 1990)
   7 Net N imports in commodities and fertilizer trade
(FAO - FAOSTAT)
   8 Synthetic N fertilizer use (FAO - FAOSTAT and
Association of American Plant Food Control Officials -
AAPFCO)
   9 Manure N production (USDA census of agriculture,
literature coefficients)
   10 Human waste N (U.S. Census Bureau population
census, literature coefficients)
   11 Surface water N flux (USGS SPARROW model,
after Alexander et al., 2008)
2.2. Sources of New Nr to
the Environment
   Creation of "new" Nr in the environment refers to
Nr that is either newly fixed within or transported into
the United States. This "new" Nr highlights where Nr
is introduced into ecosystems. New Nr arises from
fossil fuel combustion, food production, and materials
production (Table 1).
   Fossil fuel combustion emits Nr (mostly NOX) to
the atmosphere. Fossil fuel combustion introduces 3.5
Tg N/yr and 1.9 Tg N/yr of NOX-N to the atmosphere
from transportation, and utility/other industry sources,
respectively  (Table 1). Another 0.2 Tg N/yr of NH3-N and
0.1 Tg N/yr of N2O-N is emitted from the same sources
(Table 1). Thus the total amount of Nr created by fossil
fuel combustion is 5.7 Tg N/yr,  of which > 90% is in the
formofNOx-N.
   Synthetic Nr fertilizers are typically produced by the
Haber-Bosch process and used primarily in agriculture
to  support food production. Production of fertilizers
within the U.S. introduces Nr into U.S. terrestrial
landscapes at the rate of 9.4 Tg N/yr, and net imports
of fertilizer via world trade introduce 5.8 Tg N/yr. Of
this total (15.2 Tg N/yr), 9.8 Tg N/yr is used as fertilizer
on farms and 1.1 Tg N/yr is used on non-farms (i.e.,
residential and recreational turf-grass and gardens, and
in  explosives used by the mining industry), and 4.2 Tg
N/yr is introduced for non-fertilizer uses, such as for
production of plastics, fibers, resins, and for additives to
animal feed  (Table 1).
   Additional Nr is introduced into the U.S. from C-BNF
by agricultural legume crops such as soybean and alfalfa
(7.7 Tg N/yr), and from imports of N contained in grain
and meat (0.15 Tg N/yr) (Table  1).
   Thus in 2002,  anthropogenic activities introduced a
total of 29 Tg N into the U.S., mostly in support of food
production, although turf production, industrial uses
and fossil fuel combustion were also important sources.
Natural sources of Nr in the U.S. are biological nitrogen
fixation (BNF) in unmanaged landscapes, and lightning.
The former contributes 6.4 Tg N/yr (Table 1) and the
latter 0.1 TgN/yr. Clearly, anthropogenic activities
dominate the introduction of Nr into the U.S.
   Losses of Nr to the environment in the U.S. occur
during fossil fuel combustion and food production.  The
former occurs immediately, as Nr formation during
combustion is inadvertent and the Nr, primarily as NOX,
is emitted directly into the atmosphere. The latter occurs
through all stages of food production and consumption.
The following four Sections (2.2.1-2.2.4) of this report
document the magnitude of the losses of Nr to the
environment from the various components of both energy
and food production.

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2.2.7. Nr Formation and Losses to the
Environment from Fossil Fuel Combustion
   Fossil fuels such as coal, petroleum, and natural gas
provide about 80% of all energy production in the U.S.
(based on year 2000). When these fuels are burned at
high temperatures, NOX is formed. The source of N is
either the N contained in the fossil fuel or the N2 that
makes up about 80% of atmosphere. Fuel-derived N  is
important in the case of burning coal (which contains
N), while atmospheric-derived N2 is transformed to NOX
during higher temperature processes that occur when
gasoline or diesel fuel is burned in motor vehicles (Table
1). As Figure 3 indicates, in the U.S., highway motor
vehicles account for the largest anthropogenic source of
NOX (36%), followed by off-highway vehicles, electric
utilities, and industrial processes.  Emissions from aircraft
make up only about 1% of the U.S. total for NOX, but a
large proportion of this is released in free troposphere
where lifetimes are long and adverse impacts wide-
ranging. As such, continued reductions are encouraged
(e.g., EPA Regulatory Announcement: New Emission
Standards for New Commercial Aircraft Engines, www.
epa.gov/oms/regs/nonroad/aviation/420f05015.htm).
   Figure 3 also illustrates that the amount of NOX (reported
as metric tons of N) released from various fossil fuel sources
has decreased dramatically from 1970. Total emissions were
on the order of 7,400 metric tons in 1970 and decreased
to 5,900 in 2002, with further decreases in 2006 to 5,030
metric tons. Overall this represents a decrease of over 30%.
The top sources (highway vehicles, off-highway vehicles,
electric utilities, and other industrial and combustion
systems) show decreases of 15-30% from 1990 to 2002
(Figure 4). Reductions were the highest for "other" systems
followed by electric utilities. These decreases are most likely
the result of changes in regulations and control technologies
for these stationary systems. More recent preliminary
information provided by EPA indicates that electric power
NOX emissions may have decreased 70% between 1990 and
2009 and that the electric power sector's NOX emissions
now account for about 12% of anthropogenic NOX
emissions in the U.S. (U.S. EPA, 2010b). To a lesser extent,
changes in highway vehicle regulations and the removal
of older fleets from the road has resulted in a decrease of
approximately 15%. This decrease however, is accompanied
by an increase in miles traveled, which suggests that the
actual decrease in a single vehicle is larger. Off-highway
vehicles showed an increase in emissions, potentially due to
better quantification of these sources. Such sources include
locomotives and marine engines. EPA is in the process of
implementing a number of regulations that will reduce NOX
emission from mobile sources (see Appendix F). Figure
5 (provided by EPA) projects decreases in U.S. mobile-
source NOX emissions. The implications of these recent
regulations are not reflected in the quantitative analyses
presented in this report. However, additional control of
these and other sources could further decrease emissions.
In fact, technological development in the locomotive
industry shows that decreases of approximately 70% are
possible. Further decreases would require more innovative,
expensive methods such as selective catalytic reduction
o
z
ID
(3
O

'o
•c
o
E
&
T3
on
                P.rc.rt eh*!*)* I torn 1990 to 2002

Figure 4: Percent reductions in NOX emissions,
1990-2002, from different sources
(off-road, on-road, power generation, etc.)

Data source: www.epa.gov/ttn/chief/trends. More recent
preliminary information (U.S. Environmental Protection
Agency, 201 Ob) indicates that  electric power NOX
emissions may have decreased 70% between 1990 and
2009.

-------
(SCR) with urea injection. Engine manufacturers are also
investigating using SCR systems for diesels. However, it
must be noted that these systems emit small amounts of
NH3 and must be operated properly to avoid trading off
NOX emissions for NE^ emissions.
   It should also be noted that it is difficult to control
nitrogen emissions by regulating one source solely. As seen
in Table 2 (data taken from 2001 for illustrative purposes
have been reported with a higher degree of precision
than is likely to be known), in Texas, fuel combustion
Figure 5: Mobile source NOX emission
inventories
Source: Figure provided by Margaret Zawacki of the U.S.
EPA Office of Transportation and Air Quality. Inventory
data used to develop this figure are available in EPA's final
rule, Control of Emissions from New Marine Compression-
Ignition Engines at or Above 30 Liters per Cylinder (www.
epa.gov/otaq/regs/nonroad/marine/ci/420r09019.pdf),
except for onroad emissions, which EPA generated by
running MOVES2010 (www.epa.gov/otaq/models/moves/
index.htm) at the national-month level.
sources are on the same order as emissions from highway
vehicles. By comparison, in California, highway and
off-highway vehicles are the dominant source of nitrogen
emissions (over 75%). These results can be attributed to
differences in the two states' industries and power plants.
In Texas,almost 40% of the power generation is from
coal-fired plants. On the other hand, California imports
most of its coal-fired power and generates its own power
predominantly from other sources, such as natural gas
(50%), hydro and nuclear (33%). Table 2 also shows
emissions in Florida, Ohio, and Illinois. The emission of
NOX from highway vehicles is likely related to population.
For example, the estimated population of California in
2006 was 36.4 million people versus Ohio and Illinois
which are on the order of 11-12 million.

2.2.2. Nr Inputs and Losses to the Environment
from Crop Agriculture
  Agriculture uses more Nr and accounts for more
Nr losses to the environment than any other economic
sector. Synthetic fertilizers are the largest sources
of Nr input to agricultural systems. The next largest
source is N fixation in cultivated croplands  (Table 1).
The major pathways by which Nr is lost from these
systems include NOs" losses from leaching, runoff and
erosion and gaseous emissions via volatilization of NH3
and NOX and nitrification/denitrification. Similar loss
pathways occur for Nr that cycles through livestock
systems, which also account for a large portion of Nr
flux (predominantly as NH3)  in animal agricultural
systems (Aneja et al., 2006).  Therefore,  assessment of
Nr impacts on the environment and development of
strategies to minimize negative impact should be based
on a thorough understanding  and accurate accounting
    Table 2: Examples of multiple sources from states with high NOX emissions based on 2001 data,
                                         and tons of NOX as NO2

Fuel Combustion - Electric Util.
Fuel Combustion - Industrial
Fuel Combustion - Other
Industrial Processes
Highway Vehicles
Off-Highway Vehicles
Miscellaneous Sources

TOTAL, metric tons
TX CA
91,441
98,978
9,222
25,584
164,937
106,162
4,807

501,151
8,441
31,237
21,407
13,786
182,471
85,064
7,882

350,301
mm
87,489
11,792
5,707
5,933
116,889
38,475
13,110

279,778

93,792
17,300
12,974
8,123
83,593
46,239
1,526

263,561

59,124
26,481
10,894
7,122
78,278
52,797
999

235,817
Source: Data derived from the 2001 information obtained at: www.epa.gov/air/data/.

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of Nr fluxes in both crop and livestock systems, and
the trends in management practices that have greatest
influence on Nr loss to the environment from these
systems (Aneja et al, 2008a,c).
   As previously noted, in the past 60 years N fertilizers
have had a beneficial effect on agriculture both nationally
and globally by increasing crop yields. However, the high
loading of Nr from agricultural nutrient sources has led to
deleterious effects on the environment, such as decreased
visibility from increased aerosol production and elevated
N concentration in the atmosphere, ground, and surface
waters (Galloway et al., 2003).

Nitrogen fertilizer use information
   Obtaining accurate data on fertilizer use is a critical
first step in understanding Nr cycles in agriculture. There
are several sources of data reporting fertilizer usage
but it is not clear whether data quality are sufficient
for assessing environmental impact. Although the
Uniform Fertilizer Tonnage Reporting System (UFTRS)
was developed to collect fees to fund the consumer
protection mission of state chemists and fertilizer
regulatory control officials, it also provides data on
fertilizer sales in many states, which in turn are used by
many agencies and environmental scientists to estimate
consumption and use of nitrogenous fertilizers in the
U.S. The Association of American Plant Food Control
Officials (AAPFCO) tallies and publishes the statewide
fertilizer sales data annually (Terry and Kirby, 2006).
Information published by AAPFCO is a widely used
source of fertilizer use data. It is typically assumed that
fertilizers are used in the  same region in which they
were sold. The annual data published by AAPFCO,
which are based  on commercial fertilizer sold (and taxed
in some but  not all states), is the only state-level data
source available. This state-level data source includes
fertilizer sales for both agricultural and non-agricultural
purposes. These  state-level data must then be allocated
to counties,  regions, or watersheds in the states, and the
algorithms used for this process are based on a number
of assumptions that address dealer/farmer storage,
inventories,  and  cross-state sales issues (personal
communication,  Stan Daberkow, USDA-ERS).
   The U.S.  Department of Agriculture (USD A)
National Agricultural  Statistics Service (NASS)
fertilizer usage data represents another source of
information derived from farmer "agricultural chemical
use" surveys that provide information in six categories:
field crops, fruits and vegetables, nurseries/floriculture,
livestock use, and post-harvest application. NASS
periodically collects fertilizer, pesticide, and pest
management data from a stratified random sample of
farmers at the field level. The NASS report represents a
useful data source but also would require extrapolation
across reported crop acreage  to represent a complete
sample of application rates.
   The UFTRS was not designed to track the source of
inorganic nutrients applied to agricultural land on the
geographic scale needed for watershed modeling. The
system only tracks sales of synthetic fertilizers and not
manure or biosolids applied to farmland. In addition,
geographical data associated with each sale may or may
not be near the actual point of application. However,
given either regulatory or legislative changes (data
reporting is mandated through each state's fertilizer law),
it could be possible to refine the current system used by
each state's department of agriculture to generate more
precise data for improved modeling of watershed-scale
nutrient mass balances. Those changes would help target
interventions and extension programs to improve nutrient
management and reduce nutrient losses. The lack of
potential funding and the necessity to coordinate all the
states involved limit the practicality of such an approach.
   State departments of agriculture have considered ways
to improve the reporting system. Such improvements
could include:
1. An assessment to determine the needs for fertilizer
  usage data, the accuracy of the current data collection
  methods, and whether methods require revision to meet
  highest priority needs
2. Improvements in the database format and web-based
   access
3. The identification of funding sources to support
   development of a more accurate, accessible, and
   comprehensive database system
4. Education and outreach to improve precision of
   reported fertilizer tonnage, including a clear distinction
  between nutrients used in crop, livestock, and non-
   agricultural operations
   In addition, the information could be refined to reflect
site-specific data layers, although that would require
development of a geospatial framework (and legal
authority) to encourage reporting at the retail level where
it is possible to collect geographic information.
   The Chesapeake Bay watershed provides a good
example of the fertilizer data dilemma. While the fertilizer
tonnage that is currently being utilized to calibrate the
Chesapeake Bay Program Watershed Model is relatively
accurate, the county-specific tonnage may have an accuracy
of only ±20 to 50% (Chesapeake Bay Scientific Technical
Advisory Committee, 2007). For example, in a recent
year, 17% of the reported tonnage was reported without an
identified use, and there are indications some tonnage may
have been reported more than once through the distribution
chain (Chesapeake Bay Scientific Technical Advisory
Committee, 2007). It is also possible that fertilizer reported
for crop agriculture may actually have been used for lawn
and turf, forestry, or other non-agriculture applications.
   Nitrogen fertilizer application data collected on a
specific crop-by-crop basis that can be associated with

-------
crop yields and location are essential for assessing both
use patterns and efficiency. As previously mentioned, the
USDA-NASS maintains a database on N fertilizer rates
applied to the major crops (corn, wheat, cotton, soybeans,
and occasionally other crops). The data are gathered from
farmer surveys conducted every other year. These data
represent a source of useful information (Protocols for
Farming Reporting, Mark R. Miller, USD A, NASS).
   Data derived from NASS farmer surveys include six
categories: field crops, fruits and vegetables, nurseries/
floriculture, livestock use, and post-harvest application.
For each group, NASS collects fertilizer, pesticide, and
pest management data on a stratified random sample of
farmers at the field level. One field represents an entire
farm for each sample in the field crops survey. Fruit and
vegetable information are collected for the entire farm. If
the field chosen for sampling has had manure applied in
conjunction with inorganic fertilizer, only the inorganic
portion will be reported because the survey does not ask
about manure.
   Certain core crops are  surveyed every other year on
an even/odd basis and states are selected to cover at least
80% of planted acres. There has been a recent five-year
gap in the collection of NASS data for corn and other
commodity crops.  NASS was scheduled to resume
coverage of corn and other commodity crops in 2010.
This is a critical data gap and it is a problem given the
large changes in corn price and production area during
this period. Those data are needed to make progress in
assessing fertilizer use and efficiency for major crops
in the U.S. USDANASS must resume theiryearly data
  or.
  H
  N
14  n

12  -

10  -
 8  -

 6  -

 4
  '€   0
        1955  1965  1975  1985  1995  2005
Figure 6: Fertilizer consumption in the United
States,  1960 to 2006
Source: Slater et al., 2010. Reprinted with permission
from the Association of American Plant Food Control
Officials.
collection for commodity crops (Chemical Use Survey).
Potential environmental impacts of increased N inputs
associated with expanded corn acreage for biofuel
production cannot be properly evaluated in the absence of
such critical nutrient management data.
   Based on the NASS survey data, the USDA Economic
Research Service released a report on fertilizer use
that provided data on fertilizer consumption and type
of fertilizer used from 1960-2006 (Figure 6) and types
of fertilizers used (Table 3) (USDA, 2008). The share
of crop area receiving fertilizer and fertilizer use per
receiving acre, by nutrient, are presented for the major
producing states for corn, cotton, soybeans, and wheat.
Additional data include fertilizer farm prices and indices
of wholesale fertilizer price.
   Finding 1: Crop agriculture receives 60% of U.S.
annual new Nr inputs from anthropogenic sources (9.8
Tg from N fertilizer, 1.1 Tg from crop BNF versus 29
Tg total) and accounts for 58% (7.6 Tg) of total U.S.
Nr losses from terrestrial systems to air and aquatic
ecosystems. However, current monitoring of fertilizer use
statistics by federal agencies is inadequate to accurately
track trends in quantities and fate of N applied to major
crops and the geospatial pattern by major watersheds.
   Recommendation 1:  The Committee recommends
increasing the specificity and regularity of data
acquisition for fertilizer application to major agricultural
crops in terms of timing and at a sufficiently small
application scale (and also for urban residential and
recreational turf) by county (or watershed) to  better
inform decision-making about policies and mitigation
options for reducing Nr load in these systems,  and to
facilitate monitoring and evaluation of impact from
implemented policies and mitigation efforts.

Nitrogen fertilizer use  efficiency
   Nitrogen fertilizer use efficiency (NFUE) is  critical
because higher use efficiency leaves less N remaining
to create potential environmental problems. Here and
throughout this report we define NFUE as the grain
yield per unit of applied N, which is the product of two
parameters: (1) the proportion of applied N fertilizer that
is taken up by the crop, or N fertilizer recovery  efficiency
[recovery efficiency (RE) in kg N uptake per kg N applied],
and  (2) the physiological efficiency with which the N
taken up by the crop is used to produce economic yield for
crops such as grain or fruit [physiological efficiency (PE)
in kg yield per kg N uptake] (Cassman et al., 2002)13. All
else equal, when higher NFUE is achieved without yield
reduction, the crop takes up more of the applied N and
incorporates it into its biomass, which leaves less of the
applied Nr at risk for losses via leaching, volatilization, or
denitrification. Fixen (2005) reports that there is substantial
opportunity for increasing NFUE through development
13 N fertilizer use efficiency (NFUE) is calculated as the ratio of grain yield to the quantity of applied N fertilizer (kg grain/kg applied N).

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           Table 3: Types and amount of nitrogen fertilizers used in the United States in 2002
Synthetic Nitrogen Fertilizers
Other
Urea
N Solutions
Anhydrous NH3
Ammonium phosphates and N-P-K blends
Ammonium sulfate, aqua ammonia, ammonium
nitrate, and other nitrate and ammonical N
fertilizers
Total *
Tg N/year
0.21
2.21
2.55
2.88
2.28
0.76
10.89
% of total
2
20
23
26
21
7
100
 Data from Terry and Kirby, 2006
 * Because of number rounding, the sum of individual percentages does not equal 100%.
and adoption of more sophisticated nutrient management
decision aids.
   In most cropping systems, RE is the most important
determinant of NFUE. A recent review of RE for cereals
based on field studies around the world, mostly conducted
on "small-plot" experiments at research stations, reported
mean single year RE values for maize, wheat and rice of
65%, 57%,  and 46%, respectively (Ladha et al., 2005).
However, crop RE values based on actual measurements
in production-scale fields are seldom greater than 50%
and often less than 33%. For example, a review of RE in
different cropping systems estimated average recoveries
of 37% for  maize in the north central U.S. (Cassman et
al., 2002). It is also important to note that soil N provides
the majority of the N taken up by most crops  grown on
soils with moderate to good soil fertility. For maize in the
U.S. Corn Belt, for example, 45-77% of total N uptake
was estimated to  come from soil N reserves, based on
experiments from research stations (Sawyer et al., 2006).
Therefore, highest N efficiency and economic return on
N inputs are achieved when the amount and timing of
applied N is synchronized with the  availability of soil
N throughout the growing season to minimize both the
quantity of N input required and the N losses  from soil
and applied N sources.
  However, there are relatively few data that provide
direct measurement of N fertilizer recoveries  by major
field crops under production-scale conditions. Reducing
the uncertainty in estimates of N fertilizer RE is
fundamental for prioritization of research and education
investments, both in the public and private sectors. While
management can substantially improve RE on average,
in any given year weather will always be an uncontrolled
factor that can significantly influence system efficiency.
Weather can influence system efficiency through effects
on crop growth vigor and ability to acquire applied
nutrients and through losses of nutrients due to runoff,
denitrification, and leaching that can occur in periods of
excessive rainfall.
   Although total N fertilizer use in the U.S. has increased
more slowly in the past two decades (Figure 6), yields
of all major crops have continued to increase. Because
crop yields are closely related to N uptake (Cassman et
al., 2002), these trends imply a steady increase in NFUE
and reduced N losses to the environment because more
of the applied N is held in crop biomass and harvested
grain. Greater NFUE has resulted from two factors.
The first factor is a steady  improvement in the stress
tolerance of corn hybrids (Duvick and  Cassman, 1999)
that increases crop growth rates and allows sowing at
higher plant densities, which together accelerate the
establishment of a vigorous root system to intercept and
acquire available N in the  soil profile. The second factor
is the development and adoption of technologies that
may improve the congruence between  crop N demand
and the N supply for indigenous soil resources and
applied N. Examples of such technologies include soil
testing for residual nitrate  and adjusting N fertilizer rates
accordingly, split N fertilizer applications, fertigation
(the application of nutrients through irrigation systems),
site-specific management,  and new fertilizer formulations
(e.g., controlled release, nitrification inhibitors). For
maize, which receives the  largest share of total N fertilizer
in the U.S. (44% in 2005), NFUE decreased markedly
in the 1960s because N fertilizer rates rose more quickly
than maize yields. However, with recognition of negative
impact from over-application of N and associated N
losses to the environment (especially with regard to water
quality) investment in research and education to improve
N fertilizer efficiency resulted in more  than 50% increase
in NFUE from 1974-76 to 2002-05 (Figure 7). Similar

-------
                 70
                 65
                 60
              (U
              Q.
                 45
                 40
                 35
                                        K" •  •
                   1960   1965   1970   1975   1980   1985   1990   1995  2000   2005
Figure 7: Trends in corn grain produced per unit of applied fertilizer N (NFUE) in the United States
Source: Adapted from Fixen and West, 2002 (Figure 6). Adapted with permission; Copyright 2002, Springer
Science+Business Media B.V. on behalf of the Royal Swedish Academy of Sciences.
improvements have been documented for rice production
in Japan and for overall crop production in Canada.
  Despite these steady improvements, current levels
of N fertilizer uptake efficiency appear to be relatively
low, although data from production-scale studies are
few (Cassman et al., 2002). Most farmers do not use
best management practices (BMPs) with regard to
nitrogen fertilizer management. For example, a recent
U.S. Department of Agriculture Economic Research
Service (USDA-ERS) Agricultural Resources and
Environmental Indicators (AREI) report indicates that a
majority of farmers still apply N in the fall, which gives
the lowest fertilizer uptake efficiency and highest Nr
losses compared to application in spring or during the
crop growth period (USDA, ERS, 2006). This situation
suggests substantial potential for improvement in NFUE
and an associated reduction in Nr losses from crop
agriculture, especially for maize in the warmer portions
of the Corn Belt and other southern and southeast areas
where maize is grown. One potential development is
the use of controlled release fertilizers that release N in
congruence with crop demand during the growing season.
Although such fertilizers are already in use on high value
horticultural crops, they are currently too expensive for
lower value commodity grains  such as corn, rice, or
wheat. Control of N release should result in higher NFUE
where there is high risk for N losses in cereal systems
that receive the total amount of applied N in one or two
large doses. Production-scale field studies are needed
to document the benefits of this and other innovative
technologies to improve NFUE.
  As producers have significantly increased yields
in commodity crops over the past 25 years, it is
questionable whether university recommendations for
nutrient applications are still current. Many university
recommendations are now 20 to 25 years old. As a
corollary to this problem, numerous environmental
models of nutrient pollution are still utilizing older yield
estimates, which often underestimate crop nutrient uptake
and overestimate nutrient losses (Burgholzer, 2007).
   A systematic effort needs to be made to update data on
crop yields used to estimate nutrient losses.  The concept
of NFUE should be emphasized as a way to address the
need to balance economic and environmental goals. In
fact, the development and adoption of technologies that
improve nitrogen fertilizer efficiency can contribute to
more profitable cropping systems through a reduction
in fertilizer costs. For example, average NFUE in the
U.S. required 1.0 kg of applied N to produce 43 kg of
grain yield in the 1974-1976 period, whereas that same
amount of N produced 65 kg of grain in the 2003-2005
period (data taken from Figure 7). This gain in efficiency
means that it is possible to achieve the 2004 U.S. average
corn yield of about 150 bushels per acre  (9,444 kg/ha)
with 144 Ibs per acre (161 kg/ha) of applied N fertilizer
(based on the most recent NFUE achieved by U.S. corn
producers) versus about 200 Ibs per acre (224 kg/ha) of
N fertilizer at the 1980 efficiency level.
   Nitrogen costs have become extremely volatile,
mirroring natural gas prices. In late 2008, N  fertilizer
prices were more than double the 2006-2007 N fertilizer
prices. More recently, N fertilizer prices have fallen back
to two thirds of the high following the decline of natural
gas prices. If corn can be sold for $4.00 per bushel (25.5
kg) and N costs $0.40 a pound (0.45 kg),  this is a 10
to 1 price ratio - the same as the $2.00 corn and $0.20
nitrogen ratio that was typical from 2000 to 2005. There
are also other critical factors in a farmer's N application
decisions, such as yield at the margin and weather. In the
Corn Belt, one or two years in five may provide extremely

-------
favorable weather for corn production. A producer may
view applying some extra N, hoping for good weather,
as a reasonable economic gamble. If the yield response
is more than half a bushel (12.7 kg) of corn per pound
(0.45 kg) of N at the margin or if there is more than one
extremely good year in five, the farmer benefits.
   Realistically, few farmers calculate their marginal
returns from additional N in good years versus average,
but the high corn-to-fertilizer price ratio encourages some
farmers to plan for a good year and consider a larger N
application than might otherwise be appropriate for the N
utilization in the four years of lower yield. This presents a
real dilemma if the policy goal is to reduce N transfers to
the environment, especially in the four years of average
or lower yields. Meeting this challenge will require
approaches such as the development of real-time, in-
season, decision-making tools that allow crop producers
to use N fertilizer rates for average yields at planting and
during early vegetative growth, and a final top-dressing
as required to meet any additional N demand above this
amount due to favorable climate and soil conditions
that support higher than average yields (Cassman, 1999;
Cassman et al., 2002). Robust crop simulation models
using real-time climate data at a relatively localized
geographic scale will be  required to develop such tools.
   Another option is to develop new, alternative crop
production systems that  require less N fertilizer. Such
systems may employ legume cover crops, more diverse
crop rotations, and tighter integration between crop and
livestock production to achieve greater reliance on N
inputs from legume N fixation and recycling of N in
manure and compost. At issue, however, is whether such
systems actually reduce  Nr losses to the environment
because the same loss mechanisms and pathways operate
on N from both commercial fertilizer and organic
sources. Also at issue is  the indirect land use change
impact from widespread adoption of these more diverse
cropping systems because they have reduced crop yields
per unit land area compared to more simplified crop
rotations such as corn-soybeans that receive N fertilizer.
Lower yields would require more land in production to
meet food demand. Therefore, a key issue is whether the
tradeoff in reduced N fertilizer inputs to more diverse
crop rotations with organic N inputs would actually
result in less Nr losses to the environment compared to
conventional cropping systems that require less land to
produce the same amount of crop output.
   Another approach to reduce Nr losses from agriculture
would be to shift and/or adjust cropping systems across
the landscape. This would involve changes in land use as
well as crops. This approach for parametric reductions
in nitrogen was analyzed extensively in the Gulf of
Mexico Hypoxia Assessment (Doering et al., 1999). As
part of a modeling exercise to address  opportunities and
consequences for reducing Nr, crop rotations as well as
tillage practices and fertilizer inputs were adjusted to
meet successive constraints on excessive Nr while also
maximizing consumer and producer welfare to the extent
possible. The model favored those crops and cropping
practices that had lower Nr leakage. Where the model
could not find a crop production system at given locations
that allowed positive net returns to the land, that land
was taken out of production. At a 20 percent Nr reduction
scenario, crop acreage was reduced by about 6%. This
analysis was based on crop genetics, rotations, and tillage
practices as of the 1990s.

Unintended impacts of lower application rates of
nitrogen for crop production
   Crop production and environmental quality are
potentially lost or gained at the expense of each other.
Although leakage of N from crop production systems
cannot be eliminated, N losses can be minimized
substantially.  One way to decrease leakage is to apply
less N fertilizer to croplands. For example, Hu et al.
(2007), using the SWAT model, predict that decreasing N
fertilizer application rates by 10 to 50% of those used in
the 1990s in the upper Embrarras River watershed in east
central Illinois would decrease NCV output to the river
by 10 to 43%. This simple "solution" can cause problems
for crop production as yields and crop quality  (protein
content) may  decrease, causing economic loss to the
farmer, decreased food quality for the consumer and, at a
global scale, a reduction in food security.
   Cropping systems managed in a consistent  manner
over time reach a functional equilibrium between nitrogen
inputs and outputs. Because crop yields are closely
linked to the quantity of N accumulation in above ground
biomass at maturity  (Cassman et al., 2002), there would
be a proportional decrease in crop yields in response
to a decrease  in the amount of N fertilizer application.
The magnitude of this yield reduction would depend
on the magnitude of decrease in the rate of applied N
and the efficiency of N uptake from the applied N, as
well as interrelationships with the availability  of other
nutrients. Hence, yield reductions can be mitigated,  or
even eliminated,  if methods and fertilizer  formulations
used in fertilizer-N application increase the efficiency of
N uptake to offset the reduction in the amount of applied
N. It is also important to note that reduced or insufficient
N rates for crop production risk impairment of long-term
soil productivity. Jaynes  and Karlen (2005) reported that
N rates below the agronomic and economic optimum
could degrade the soil resource and decrease soil organic
matter over time. Thus care must be exercised in any N
rate adjustments to protect soil productivity and to support
soil resource sustainability.
   Another concern associated with decreasing N
fertilizer input to cereal crop production is the effect on
crop quality. In Denmark, for example, national efforts
to decrease Nr losses to the environment  resulted in a
large reduction in use of N fertilizer in crop production
(Figure 8). This situation occurred as part of Denmark's

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LJ_


n


n
n



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o

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* • • * *
* * * *
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1986 1990 1994 1998 2
Year
* Synthetic N • Livestock Manure N






002









F/gure 8: Synthetic fertilizer and livestock manure
N used as fertilizer in Denmark 1985-2003
Source: IFA, 2004. Reprinted with permission from the
International Fertilizer Industry Association.

response to the European Union Nitrate Directive.
Synthetic fertilizer N use in Denmark decreased from
approximately 0.4 Tg N in 1991 to 0.2 Tg N in 2002.
Animal manure N application decreased from 0.25 Tg
N to approximately 0.24 Tg N during this time period.
Despite this large decrease in N fertilizer use, cereal
crop yields remained relatively constant (data not
shown). But while grain yields were maintained, there
was a decrease in grain quality as determined by protein
content (Figure 9). Grain protein  content in wheat is
critical for determining its quality for bread. In the
U.S., a grain protein content of 12% is considered the
threshold for good quality bread wheat, and N fertilizer
application rate has a large influence on determining this
trait (Cassman et al.,  1992). As can be seen, grain protein
content has declined from 12 to 10% in Denmark over
the same period of lower fertilizer application rates.
     13.d
  ro

  I
     12.0.
     11.0.

     10.0.
       9 a
               1990
                             1995
                                          2000
Figure 9: Protein content of cereal grain
in Denmark
Source: IFA, 2004. Reprinted with permission from the
International Fertilizer Industry Association.
   Such trends raise several questions if declines
continue or are found to be widespread. What is the cost
to the farmer (considering human nutrition and end-use
value costs)? Do these costs offset the environmental
benefits created by decreasing N flows from crop
production areas? What would be the regional  and global
impact if similar reductions in nitrogen fertilizer inputs
to agriculture were put in place in developed countries
that represent the largest source of grain exports to
international markets?
   As previously discussed, it is possible to increase
crop yields without an increase in N fertilizer input if the
methods used to apply N are modified to improve NFUE
as illustrated by U.S. maize yield trends and fertilizer
use (Figure 7). However, at some point continued yield
increases will require additional N input because of
the tight relationship between yield and crop N uptake
requirements. The goal of N fertilizer management
should therefore be to achieve highest possible NFUE
while also sustaining crop yield increases adequate to
meet food demand without need for a large expansion of
crop production area. In fact, studies that evaluate NFUE
in production agriculture find a wide range in NFUE
among farmers. For farmers who achieve high NFUE, a
reduction in N fertilizer rate would reduce yields.  Hence,
mandating an across-the-board reduction in N fertilizer
use penalizes the good farmers who achieve high NFUE
as their yields would likely fall while it would  have little
effect on yields of farmers who currently over fertilize
(Cassman et al., 2003; Dobermann and Cassman,  2004).
   Finally, if yields were significantly reduced as a result
of lower N fertilization rates, more land may need to
be brought into production. Because nearly all prime
agricultural land is already used for crop production,
expansion of crop area will most likely occur on
more marginal land, such as the land currently  in the
Conservation Reserve Program (CRP). Such conversion
would have two negative results. First, additional  N
losses from these acres would occur due to relatively
low N fertilizer efficiency that typically occurs on
marginal land that has multiple soil constraints to  crop
growth and yield. Second, conversion from CRP to crop
production would result in loss of soil organic  matter that
accumulates under grassland, which in turn would lead to
increased greenhouse gas emissions from agriculture.
   Finding 2: Nr inputs to crop systems are  critical to
sustain crop productivity and soil quality. Moreover,
given limited land and water resources, global population
growth, and rapid economic development in the world's
most populous countries, the challenge is to  accelerate
increases in crop yields on existing farm land while
also  achieving a substantial increase in N fertilizer
uptake efficiency. This process is called "ecological
intensification" because it recognizes the need to meet
future food, feed, fiber, and energy demand of a growing
human population while also protecting environmental

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quality and ecosystem services for future generations
(Cassman, 1999). More diverse cropping systems with
decreased Nr fertilizer input may also provide an option
to increase NFUE on a large scale if the decrease in
Nr losses per unit of crop production in these diverse
systems can be achieved without a decrease in total food
production, which would trigger indirect land use change
to replace the lost production and negate the benefits.
However, crop cultivars and agronomic practices are
changing rapidly, which changes N requirements, but
current efforts in research, extension, and conservation
programs on N management within these rapidly
evolving systems are not adequate to meet the challenge
of providing better information to increase NFUE.
   Recommendation 2: To obtain better information
on Nr inputs and crop productivity, the Committee
recommends that:
   Recommendation 2a: Data on NFUE and N mass
balance,  based on direct measurements from production-
scale fields, should be generated for the major crops
to identify which cropping systems and regions are of
greatest concern with regard to mitigation ofNr load and
to better focus research investments, policy development,
and prioritization of risk mitigation strategies.
   Recommendation 2b: Efforts at  USDA and
universities should be promoted to: (i) investigate means
to increase the rate of gain in crop yields on existing
farm land while increasing N fertilizer uptake efficiency
and (ii) explore the potential for more diverse cropping
systems with lower N fertilizer input requirements so long
as large-scale adoption of such systems would not cause
indirect land use change.
   Recommendation 2c: EPA should work closely with
the USDA, Department of Energy, and the National
Science Foundation, and universities to help identify
research and education priorities to support more efficient
use and better mitigation ofNr applied to agricultural
systems.

Biological fixation in cultivated croplands
   Reactive nitrogen is also introduced to the landscape
in significant quantities via BNF in cultivated crop
lands. Management of biologically fixed N, insofar as
it is possible, is proportionally as critical a task as the
management of synthetic N because Nr from BNF is
prone to the same loss pathways as Nr from commercial
fertilizers. To quantify BNF due to human cultivation of
crops, the Committee calculated the annual agricultural
fixation for 2002 using crop areas and yields reported
by the USDA Census of Agriculture (USDA, 2002). The
Committee multiplied the area planted in leguminous
crop species by the rate of N fixation specific to each
crop type, assigning rates based on a literature review,
as summarized in Table 4 below and  shown relative
to other inputs in Table 1. Annual nitrogen inputs to
cropping system from BNF by legume crops was 7.7
Tg N/yr in 2002, accounting for —15% of the overall Nr
inputs to the terrestrial landscape from all sources and
20% of the agricultural sources (Table 1). Soybean and
alfalfa contributions are the most important agricultural
legumes in terms of nitrogen  input and contribute 69%
of total BNF inputs in U.S. agriculture.

Emissions factors and losses to the environment
from fertilizers and organic nitrogen sources
   Agriculture is a significant contributor ofNr inputs
into the atmosphere. Nitrogen fertilizer losses vary greatly
   Table 4: Estimates of nitrogen input from biological nitrogen fixation from major legume crops,
                                            hay, and pasture
Nr fixation in cultivated croplands

Soybeans
Alfalfa
Other leguminous hay
Western pasture
Eastern pasture
Dry beans, peas, lentils
Total
production area,
Mha
29.3
9.16
15.4
161
22.0
0.88

rate, kg/ha/yr
111
224
117
1
15
90

Tg N/yr
3.25
2.05
1.80
0.16
0.33
0.08
7.67
% of total *
42
27
23
2
4
1
100
Source: Updated estimate for soybean based on a generalized relationship between soybean yield and the quantity of
N fixation (Salvagiotti et al., 2008). Other values are from Boyer et al. (2002).
* Because of number rounding, the sum  of individual percentages does not equal 100%.

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due to differences in soil properties, climate, and the
method, form, amount, timing and placement of applied N
(Cassman et al., 2002). In addition, any factor that affects
crop growth vigor and root system function also affects
the ability of the plant to recover applied N efficiently.
For example,  denitrification can range from 0% to 70% of
applied N (Aulakh et al., 1992). This process is mediated
by heterotrophic, facultative anaerobic soil bacteria that
are most active under warm, wet soil conditions; they
have low activity in dry sandy soils.
   Despite this variation, watershed, regional, and
national assessments of carbon and N cycling often
rely on average values for losses from each pathway.
For example, the Intergovernmental Panel on Climate
Change (IPCC) assumes that 1% of applied N fertilizer
(uncertainty range of 0.3-3.0%) is lost from direct
emissions of N2O at the field level due to nitrification/
denitrification. This assumption is based on analysis
of all appropriate scientific publications that report
these losses for specific crops and cropping systems
(IPCC, 2007a).  The same 1% default emission factor
for field-level N2O emission is applied to other N
inputs from crop residues, organic  amendments such as
manure, and from mineralization of native soil organic
matter. Data from scores of field studies were used to
obtain this average value. A number of recent studies
confirm that N2O losses to the environment during the
growing season at the field level can represent <1%
of the applied nitrogen - even in intensive, high-yield
cropping systems (Adviento-Borbe et al., 2006). Despite
these average values, it is also clear that N2O losses
can vary widely even within the same field and from
year to year due to  normal variation in climate and
crop management (Parkin and Kaspar, 2006; Snyder
et al., 2007). Moreover, the loss of nitrogen from
agricultural watersheds is strongly dependent on climate
change (e.g., rainfall changes). Predicted increases and
decreases in rainfall will likely have a dramatic impact
on nitrogen export from agricultural fields. For example,
precipitation is predicted to increase in the upper
Mississippi watershed and, other factors being equal, N
export  should increase (e.g., Justic et al., 1995a,b).
   Additional indirect N2O emissions result from
denitrification of volatilized NH3 deposited elsewhere
or from NO3~ lost to leaching and runoff as the Nr
cascades through other ecosystems after leaving the
field to which it was applied.  Here the IPCC assessment
protocol assumes that volatilization losses represent 10%
of applied N, and that N2O emissions for these losses
are 1% of this amount; leaching losses are assumed
to be 30% of applied nitrogen and N2O emissions are
0.75%  of that amount (IPCC, 2007a). Therefore, the
IPCC default value for total direct  and indirect N2O
emissions represents about 1.4% of the applied N from
fertilizer. By the same calculations, 1.4% of the N in
applied organic matter, either as manure or compost or in
recycled crop residues, is also assumed to be emitted as
N2O. Recent work funded by EPA used the DAYCENT
model to estimate N2O emissions from cropping systems
(Del Grosso et al., 2005). However, due to the cost of
field validation of such models, there are relatively few
validations across a representative range of cropping
systems and environment. Therefore, it is not clear that
use of such a complex model gives better estimates of
N2O emissions than the more straightforward IPCC
assessment protocol.
   Others have estimated higher average global N2O
losses of 3-5% of applied nitrogen fertilizer based on
historical changes in atmospheric N2O content and
changes in Nr production during the past 50 years
(Crutzen et al., 2008), as opposed  to the field-based
estimates that form the basis of IPCC estimates. Because
N2O is such a potent GHG, and given the more than
two-fold difference in estimates of N2O losses, there is a
critical need to improve understanding and prediction of
N2O losses from agricultural systems. N2O emissions in
the U.S. are estimated to be 0.78 Tg N/yr (Table 5) (U.S.
EPA, 2005b).

       Table 5: A/2O emissions in the United
                   States, 2002

Agricultural Soil
Management
Manure Management
Mobile Combustion
Stationary Combustion
Nitric & Adipic
Acid Production
Wastewater Treatment
Other
Total

0.54
0.03
0.09
0.03
0.05
0.02
0.02
0.78

69
4
12
4
6
2
2
100
* Because of number rounding, the sum of individual
percentages does not equal 100%.
   Biogenic NOX emissions from croplands are on the
order of 0.5% of fertilizer input - much more than this in
sandy soils and less as clay content increases (Aneja et al.
1996; Sullivan et al. 1996; Veldkamp and Keller, 1997;
Civerolo and Dickerson, 1998). However, NOX emissions
by agricultural burning are relatively unimportant.
Ammonia volatilization of N from applied fertilizer
can be the dominant pathway of N loss in rice soils and
can account for 0% to more than 50% of the applied N
depending on water management, soil properties, and
method of application (citations within Peoples et al.,

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1995, 2004). Ammonia volatilization can be of the same
range in upland cropping systems, with largest losses
occurring typically on alkaline soils (Peoples et al.,
1995, 2004). The IPCC (2007a) uses a value of 10% of
synthetic fertilizer N application and 20% of manure N as
estimates of average NH3 volatilization.
   Taken together, N losses from all forms of direct
gaseous emissions from crop production systems can
represent a substantial portion of applied N fertilizer
when soil conditions favor such emissions and there is a
lack of synchrony between the amount of N applied and
the immediate crop demand (Goulding, 2004). Therefore,
achieving greater congruence between crop demand and
the N supply from fertilizer is a key management tactic
to reduce N losses from all sources. Success in reducing
N losses and emissions from agriculture will depend on
increased efforts in research and extension to close gaps
in our understanding of N cycling and management in
crop production, especially as systems further intensify
to meet rapidly expanding demand for food, feed, fiber,
and biofuel.
   Finding 3: Nitrous oxide emissions from the Nr inputs
to cropland from fertilizer, manure, and legume fixation
represent a large proportion of agriculture's contribution
to GHG emissions, and the importance of this source of
anthropogenic GHG will likely increase unless NFUE is
markedly improved in crop production systems. Despite
its importance, there is considerable uncertainty in the
estimates of nitrous oxide emissions from fertilizer and
research should focus on reducing this uncertainty.
   Recommendation 3: The Committee recommends
that EPA ensure that the uncertainty in estimates of
nitrous oxide emissions from crop agriculture be greatly
reduced through the conduct of EPA research and through
coordination of research efforts more generally with other
agencies such as USDA, DOE, NSF and with research
conducted at universities.

Impact of biofuel production capacity on Nr flux
in agriculture
   The enormous use of liquid fuels in the U.S., the
rising demand for petroleum based liquid fuels from
countries like China and India, and the decline in
petroleum discovery all contributed to the recent record
high petroleum prices. In addition, most of the world's
petroleum reserves are located in politically unstable
areas. This has provided strong motivation for policies
promoting investment in biofuels made from corn,
oil crops, and ultimately from cellulosic materials. In
the U.S., ethanol production capacity  from corn more
than doubled from 2006 to 2009 (to a capacity of over
47 billion liters/year in January 2009). The renewable
fuels standard in the 2007 Energy Independence and
Security Act (EISA) will support another 9.5 billion
liters/year of corn-based ethanol by 2015. An additional
79.5 billion liters is to come from cellulosic ethanol
by 2022. Production of biodiesel from vegetable oils
also is encouraged in EISA, but expansion has been
slowed by the high food value of such oils. Brazil
is rapidly expanding its production of relatively low
cost sugarcane ethanol and U.S. policies continue to
be aimed at bringing about increased future biofuel
production in the U.S.
   In 2007  and 2008 petroleum prices pushed ethanol
prices high enough to  draw corn from food and feed
uses into ethanol production and contribute to the
increased price of corn. Because of the increase in
petroleum/ethanol prices and the government subsidy
for ethanol production, 30% of the corn crop was used
to produce  ethanol in 2008 (Abbott et. al., 2008). With
the subsequent collapse in petroleum and ethanol prices,
followed by corn prices, there has been unused capacity
in the U.S.  ethanol industry as the corn/ethanol price
ratio made  ethanol production uneconomic for some
firms. However, EISA is likely to lead to the production
of cellulosic materials and even some expanded corn
production for biofuels once the U.S. gets beyond
the current blending limit for ethanol (Doering and
Tyner, 2009). The higher corn prices of 2007 and 2008
resulted in  more land being planted to corn and higher
N fertilizer requirements. Corn area went from 78.41
million acres (31.73 million hectares) in 2006-2007 to
93.6 million acres (37.88 million hectares) in 2007-
2008. Reduction in soybean production accounted
for 12.01 million acres (4.86 million hectares) of
the corn expansion,  and the remaining new acreage
came primarily from reduced cotton  acreage and from
hay land and pasture. This strong response to high
demand for biofuel feedstock has led to concern about
increased pressure on the environment from biofuels.
One important factor is the increased N necessary for
growing corn and cellulosic materials (Robertson,
et. al. 2008). Biofuels production and consumption
will also  result in NOX emissions to the atmosphere.
Expansion  of corn or cellulosic materials production
into marginal lands can be even more problematic with
respect to nutrient leaching and  soil erosion. Changes
in N fertilizer prices add uncertainty to the additional
amounts  of N that may ultimately be used in biofuel
feedstock production.  Production of large  amounts of
distillers grains co-product is also changing the way
that livestock feed rations are formulated,  which in turn
could have an influence on the cycling of N in cattle
manure (Klopfenstein et al., 2008).
   In February 2010, EPA released its final rule for the
expanded Renewable Fuels  Standard (RFS2) regarding
greenhouse gas emissions from various types of
biofuels based on life-cycle analysis as required by the
2007 EISA. Standards for corn ethanol and soybean
biodiesel are based on studies that include data from
large-scale production systems because these are the
only biofuels currently produced on a large commercial

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scale. In contrast, life cycle analysis (LCA) standards
for cellulosic and other advanced biofuels were based
on data from "pilot- and bench-scale" studies, or in
many cases on hypotheses and rough estimates. While
these initial estimates meet requirements imposed
by 2007 EISA, the science underpinning life cycle
assessments of biofuel systems, including direct and
indirect land use change, are relatively undeveloped
and evolving rapidly.
   Finding 4: Rapid expansion of biofuel production
has the potential to increase N fertilizer use through
expanding corn production and its associated N fertilizer
inputs and extending cultivation for cellulosic materials
that will also need N. Distillers grains are changing
animal diets and affecting N recycling in livestock.
Both have important consequences for effective future
management of Nr, but current models and understanding
of how expanded biofuel production will affect Nr inputs
and outputs from agriculture are inadequate to guide
policy.
   Recommendation 4: EPA should work with  USDA
and universities to improve understanding and prediction
of how expansion of biofuel production, as mandated by
the 2007 EISA, will affect Nr inputs and outputs from
agriculture and livestock systems. Rapid expansion
of biofuel production has the potential to increase N
fertilizer use through expansion of corn production area
and associated N fertilizer inputs, and from extending
cultivation of cellulosic materials that will also need
N inputs. Current models and understanding are not
adequate to guide policy on how to minimize impact of
biofuel expansion on environmental concerns related
to Nr.

2.2.3. Nr Inputs and Losses  from
Animal Agriculture
   In the U.S., domestic animals produce 6.0 Tg N/yr in
manure and are the largest source of atmospheric NH3-N
(1.6 Tg N/yr) (Table 1). Livestock also contribute to
N2O-N emissions, though in much smaller proportions
(-4% of total U.S. N2O-N emissions).

Trends in Animal Agriculture
   While animal production has been increasing  since
World War II, this report emphasizes the period from
1970 to 2006. The production of chicken broilers
increased more than four fold from 1970 to 2006
(Figure 10) and milk production increased by nearly
60% in this time period (Figure 11). Turkey production
doubled and pork production increased about 25%,
while meat from cattle (beef and dairy)  remained
constant (Figure 10).
   Another trend in animal production has been for fewer
animals to produce more animal products. For example,
the 60% increase in the amount of milk produced in 2006
compared to 1970 required 25% fewer cows (Figures  11
and 12). Animal inventories declined by 10% for beef
      1970  1975  1980  1985  1990  1995 2000 2005  201C
                           Year

Figure 10: Meat production from 1970 to 2006
Source: USDA, MASS, 2007 - Census Reports. Data on
cattle were not taken after 1999.
      1970  1975  1980  1985 1990 1995 2000 2005  2010
                          Year

Figure 11: Milk production from 1970 to 2006
Source: USDA, MASS, 2007 - Census Reports

brood cows from 36 million head in 1970 to 33 million
head in 2006, and the inventory of breeder pigs and
market hogs declined 8% from 673 million head to 625
million head in the same period, even with similar or
greater annual meat production. This trend resulted from

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greater growth rates of animals producing more meat in a
shorter amount of time. In 1970, broilers were slaughtered
after 80 days on feed at 1.7 kg live weight, but by 2006
the average weight was 2.5 kg after only 44 days on feed
(USDA, NASS, 2007).
   Another trend in animal agriculture has been the
increased size and smaller number of animal operations,
which results from the mechanization of agricultural

         —»— Dairy cows, millions |  | —a • Milk.'cow (kg/cowtyr) |
    12.5 .	.	,	.	.	,	.	,	, 1 10"
                                               9000
                                               4000
       1970  1975 1980  1965  1990 1995  2000 2005 2010
                          Year

 Figure 12: U.S. inventory of mature dairy cows
 and milk production per cow from  1970 to 2006
 Source: USDA, NASS, 2007 - Census Reports
           •  Swine Operations. 1000s
          —E • Beel Operations. 10OOs
          - (f • Dairy Operations. 1000s
    2000
 £
 I
 o
    1500 -
    1000 —
     500
       1970 1975  1980  1985  1990  1995 2000 2005  201C
                            Year

Figure 13: Number of animal operations in the
United States from 1970 to 2006
Source: USDA,  NASS, 2007 - Census Reports
practices and increased specialization. There were only
7% as many swine operations and 11% as many dairy
operations in 2006 as there were in 1970 (Figure 13).
There were half as many beef operations in 2006 as in
1970, but beef operations also expanded in size while
smaller producers held jobs off the farm.
   All of these trends show an increase in management
and labor efficiency to produce a similar or greater
amount of animal products. Also, because animal
production is more concentrated on fewer farms with
greater specialization, fewer crops are produced on those
farms. As a result, it is increasingly common to have more
manure nutrients produced on a livestock farm than can
be used efficiently as  fertilizer for crops on that farm.
Therefore, unless the  manure is applied over a larger
crop area, the resulting over-application of manure on the
livestock farm can reduce the subsequent efficiency of its
utilization and result in greater nutrient losses.

Impact of livestock production trends on
nitrogen use efficiency
   The trends in livestock production have both positive
and negative environmental impacts. One of the
significant positive impacts is that with smaller animal
inventories producing greater quantities of animal
products, there is an improved efficiency of nitrogen
utilization per product produced. This effect is partly  the
result of effectively reducing maintenance requirements
during production. The requirements for feeding animals
can be divided into two components: maintenance and
production. The maintenance component is the feed
that is used to keep the animal alive and healthy so
that production is possible. The production component
includes the feed that is converted to animal protein and
waste due to the inefficiencies of these conversions. The
maintenance component depends upon the number of
animals, each animal's mass, and the time the animal is
on feed. Thus, the maintenance requirement is diluted by
faster growth rates and greater body weight  at slaughter.
The increases in production rates over time have led
to greater efficiencies in N and P utilization  for animal
production and lower amounts of nutrients excreted per
unit of animal protein produced.
   Public concerns about the potential environmental and
health effect of air emissions from CAFOs expand the
impacts of food production beyond those associated with
traditional agricultural practices (NRC, 2001; Aneja et al.,
2009). Increased emissions of N compounds from animal
agriculture into the atmosphere may lead to  increased
odor and interactions  in atmospheric reactions (e.g.,
gas-to-particle conversion) (Baek et al., 2004a; Baek  and
Aneja, 2004b). These compounds are then transported
by wind and returned to the surface by wet and dry
deposition processes,  which may have adverse effects on
human health and the environment (McMurry et. al, 2004;
Aneja et. al, 2006b, 2008a,b,c; Galloway et. al, 2008).

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   Adverse effects, further discussed in Chapter 3 of
this report, include eutrophication, soil acidification,
loss of biodiversity, and reactions that increase the
mass concentration of atmospheric aerosols (PM2 5).
Aerosol formation occurs when HNOs reacts with basic
compounds, and NH3 reacts with compounds. Ecosystem
acidification can occur when HNO3 is deposited from the
atmosphere. In addition, acidification can also occur when
NHX is deposited due to the production of HNOs from
nitrification via soil microbes. Soil acidification occurs
when HNOs or NH4+ deposits on soils with low buffering
capacity and this can cause growth limitations to sensitive
plant species.  Deposition of NCV or NH4+ also causes
eutrophication (i.e., an over-abundance of nutrients),
which can promote harmful algal growth leading to the
decline of aquatic species. In fact, volatized NCV can
travel hundreds of miles from its source, affecting local
and regional biodiversity far from its origin (Aneja et al.
2008b; James, 2008).
   The potential for reduced environmental impact from
Nr in livestock systems depends on the proportion of
the total intake attributable to maintenance  costs. The
commonly used tables for diet formulation published
periodically by the NRC for various animal commodities
can be used to track diet formulation practices and
assumptions regarding maintenance and production
requirements. About one third of the energy intake
recommended for growing broilers was assumed to
be needed for maintenance (NRC, 1994) but protein
requirements were not divided between maintenance and
production. For example, a dairy cow producing 40 kg
milk per year  would divert about 25% of its energy and
12% of its protein to maintenance (NRC, 2001).
   In terms of nutritional efficiency of a herd or flock,
maintenance of a productive phase (e.g., growth,
lactation) also requires maintenance of a reproductive
phase of the animal's life cycle. In other words, the actual
nutritional maintenance cost of a herd or flock is greater
than it is for productive individuals only. For example,
milk production requires non-lactating cows and heifers
in the herd which do not produce milk but which consume
nutrients. These additional maintenance costs are lower
for broiler flocks than for cattle.
   Finding 5: There are no nationwide monitoring
networks in the United States to quantify agricultural
emissions of greenhouse gases, NO, N2O, reduced sulfur
compounds, volatile organic compounds (VOCs), and
NH3. Satellite observations of agricultural emissions
hold promise for providing a high degree of spatial
coverage and  may complement surface observations
in this network, although robust methods are yet to be
developed. In contrast there is a large network in place to
assess the changes in the chemical climate of the United
States associated with fossil fuel energy production,
i.e., the National Atmospheric Deposition Program/
National Trends Network (NADP/NTN), which has been
monitoring the wet deposition of sulfate (SO42"), NCV,
and NH4+since 1978.
   Recommendation 5: The status and trends of
gases and particulate matter precursors emitted from
agricultural emissions, e.g., NOf and NH^ should be
monitored and assessed utilizing a nationwide network
of monitoring stations. EPA should coordinate and
inform its regulatory monitoring and management of
reactive nitrogen with the multiple efforts of all agencies
including those of the U.S. Department of Agriculture and
NSF supported efforts such as the National Ecological
Observatory Network (NEON) and the Long Term
Ecological Research Network (LTER).
Changes in feeding practices
   From 1970 to 2006, several feeding practices were
changed for diets fed to livestock (NRC, 1977, 1988,
1996, 2001). In 1989 and 1996, the NRC introduced the
idea of dividing the form of protein fed to ruminants into
that which is degraded by rumen microorganisms and that
which passes  through the rumen to be digested directly
in the stomach and small intestine. Feeding ruminants
with attention to rumen degraded and rumen undegraded
protein decreases the amount of protein fed by 10 to 15%
for a given protein requirement. For poultry and swine,
manufactured amino acids were added to diets, decreasing
the need for protein by 30%. Today, two amino acids
(lysine and methionine), coated in a way to prevent
degradation in the rumen, are sometimes added to dairy
cattle diets thereby decreasing protein intake by another
15% (NRC, 2001). Phytase added to swine and poultry
diets in the past decade has decreased phosphorus feeding
by 20 to 50% with some of the decrease attributed simply
to better understanding phosphorus requirements.
   It is difficult to estimate the combined effects
of these changes in feeding practices, but we have
calculated changes in manure N by using USD A NASS
data and assuming improvements in both production
rates and ration formulation (Table 6). In the case of
beef cattle diet formulation, the changes in feeding
practices were determined by comparing the NRC 1976
recommendations with the NRC 1996 recommendations.
Surprisingly, NRC 1996 recommended greater total crude
protein compared to NRC 1976 despite formulating for
rumen degraded and un-degraded protein and considering
amino acid content. Therefore, improved diet formulation
did not decrease N intake for beef in this time range but
the effect of reduced maintenance did improve efficiency
of N utilization.

Reduced nitrogen excretion from
increased efficiency
   Nitrogen excretion as fraction of animal production
decreased from 1970 to 2006 (Table 6). However, in cases
where the total amount of animal production in the U.S.
increased substantially (e.g., broilers), total N excretion
increased. The decrease in N excretion per unit of animal

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      Table 6: Livestock N excretion per kg production (g/kg) and per total United States (Tg/yr)
^^ra ^^B

Milk
Pork, live weight
Broilers, live weight
Beef, live weight

g/kg product
17
57
56
123
Total United
States
0.89
0.56
0.26
1.2

g/kg product
11
42
46
110
Total United
States
0.92
0.54
1.00
1.3
 *Does not include manure produced for reproduction of stock (e.g., growing dairy heifers, breeder pigs).
productivity was estimated by calculating the effects of
changes in feeding practices and reduction of maintenance
as described previously. The data in Table 6 indicate that
there has been an increase in N utilization efficiency for
livestock products.
  For broilers, data are available to more accurately
estimate the effect of changes in feeding and genetics
on N excretion over time. However, these data do not
represent the time period of interest in this report.
Havenstein et al. (1994) compared a 1957 strain of
broiler fed a 1957 diet to a 1991  strain fed a 1991 diet.
Based on the reported N intake and production data,
there was a 51% reduction in N excreted between these
diets (Kohn, 2004).
  Similarly, Kohn (2004) compared N excreted by U.S.
dairy cows in 1944 and 2001. In 1944, the historically
largest herd of dairy cattle in the U. S. (25 million cows)
produced an average of 7 kg milk per cow per day (USDA
NASS, 2007). In 2001, nine million cows produced an
average  of 27 kg milk per cow per day. Assuming the
cows in  1944 and 2001 were fed according to popular
feeding recommendations of the time, the N intakes were
360 and 490 g/day per cow, and N excretion rates (N
intake minus N in milk) were 326 and 364 g/day per cow.
Multiplying by the number of cows in the U.S., shows
that total milk production increased 40% from 52 billion
kg to 73 billion kg, while N excretion decreased 60%
from 3.0 Tg N to 1.2 Tg N, respectively.
  Table 7 provides information on manure production
from animal husbandry in the U.S. For Table 7, manure
N was calculated for all U.S. animal agriculture using
data on animal production from the 2002 Census of
Agriculture (USDA, 2002). For data on livestock
production (cattle, calves, poultry, hogs, and pigs),
manure was calculated by the methods of Moffit and
Lander (1999), following the exact methods they  had used
to compute manure from the 1997 Census of Agriculture,
but using the updated information from the 2002 Census
of Agriculture. For data on production of manure  from
other animals (horses, goats, and  sheep), the table uses
     Table 7: Manure production from animal
   husbandry in the continental United States,
                Tg N per year 2002.
  Cattle & Calves
  Poultry
  Hogs & Pigs
  Horses, Goats & Sheep
  Continental United States*
4.35
 72
0.94
0.53
0.19
6.02
 16
100
* Because of number rounding, the sum of manure
production by category does not equal the reported total
for the continental U.S.

coefficients for manure excretion as a function of average
animal weights and animal inventory, taken from Battye
etal. (1994).

Volatilization of animal waste
   Ammonia volatilization is highly variable and is
influenced by the amount of total ammonical nitrogen
(TAN), temperature, wind speed, pH, chemical and
microbiological activities, diffusive and convective
transport in the manure, and gas phase resistance in the
boundary layer above the source (Arogo  et al., 2006).
   EPA estimates annual manure N excreted in
livestock production in the U.S. for the Inventory of
U.S. Greenhouse  Gas Emissions and Sinks (U.S. EPA,
2007e). For the year 2002, these estimates  indicate that
a total of 6.8 Tg of N was excreted in livestock manure.
Only a fraction of this N (-1.24 Tg) was recovered and
applied directly as a nutrient source for crop production.
Approximately 1.8 Tg N was transferred from the
manure management systems, most likely by ammonia
volatilization. Other loss vectors include leaching and
runoff during treatment, and  storage and transport before
soil application. The remainder of the N was deposited
in pastures and rangeland or in paddocks. This N is also

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susceptible to movement into the atmosphere and aquatic
systems or incorporation into soil organic matter. By a
combination of BMPs and engineered solutions it may be
possible to reduce the emissions and discharge of odors,
pathogens, and nitrogen compounds from agricultural
operations (Aneja et al., 2008b,d).
   Total manure production reported in Table 7 in the
contiguous U.S. was estimated using USDA's method
and yields an estimate of 6.0 Tg N/yr; while EPA's
GHG inventory method in Table 8 yields a total for
the U.S. of 6.8 Tg N/yr  in 2002. The "greenhouse gas"
method suggests 13% higher manure N production. This
difference highlights uncertainty in the calculations.
Although the values in Table 8 include Alaska and Hawaii,
the values in Table 7 do not; however,  given the relatively
small amount of livestock production in those states, that
does not contribute substantially to the difference.
   Finding 6: Farm-level improvements in manure
management can substantially reduce Nr load and
transfer. While the NPDES permitting process for
CAFOs does include nutrient and manure management
plans to limit the transport of land applied nutrients
off farms, substantial off-farm transport of Nr still
occurs, especially via atmospheric transport. There are
currently very few incentives or regulations to decrease
these transfers and loads despite the  existence of
management options to mitigate.
   Recommendation 6: A policy, regulatory, and
incentive framework is needed and should be
developed to improve manure management to reduce
Nr load and ammonia  transfer, taking into account
phosphorus load issues.

2.2.4. Nr Inputs to Residential and Recreational
Turf Systems
   Turf grasses cover 12.6-16.2 million ha across the
continental U.S. (Milesi et al., 2005). The area under
turf grass is roughly the size of the New England states
and occupies an area up to three times larger than that of
irrigated corn (The Lawn Institute, 2007). The majority
of this turf area (approximately 75%) is in residential
lawns. About 80% of all U.S. households have private
lawns (Templeton et al. 1998) that average 0.08 ha in size
(Vinlove and Torla, 1995). Another approximately 15 %
of total turf grass area is in low-maintenance parks and
approximately  10% is in athletic fields and golf courses,
which often receive higher levels of N application due to
hard use conditions.
   Supplemental N fertilization is often necessary to
maintain healthy and aesthetically pleasing turf color,
high shoot density,  and the ability to resist and recover
from stress and damage. Nitrogen also may be derived
from atmospheric deposition or recycled decomposition
of soil and grass clipping organic matter. Whether these
inputs are  sufficient to  maintain lawns of adequate quality
depends on many factors including age of the turf, uses,
and expectations or goals of the homeowner or field
manager. Also, turf grasses are used to stabilize soil,
often with an erosion-prevention matrix such as organic
mats or with hydroseeding. Depending on circumstances,
these turf uses may be  temporary until natural vegetation
succeeds the turf, or may be  low-maintenance turfs that
are seldom fertilized, such as highway medians and
shoulders, grassy swales and buffers.
  Turf grass is maintained under a variety of conditions.
Approximately 50% of all turf grass is not fertilized;
the remainder is fertilized at varied intensities (A.M.
Petrovic, personal communication, June 5, 2007). The
Committee has arrayed the different turf managements
into the following three groups according to the estimated
amount of N-fertilizer applied annually (Table 9):
residential lawns maintained by homeowners (0.73
kg/100 m2), residential lawns cared for by professional
lawn care companies (2.92 [range, 1.95-7.3] kg/100 m2),
and athletic fields and golf courses (3.89 [range, 2.64-
6.64] kg/100 m2). The  estimate of total N-fertilizer used
                            Table 8: Fate of livestock manure nitrogen (Tg N)
Activity
Managed manure N applied to
major crops
Manure N transferred from
management systems
Pasture, range, & paddock
manure N
Total *
1990 1992 1994 1996
1.1
1.5
3.9
6.6
1.2
1.6
4.0
6.7
1.2
1.6
4.1
6.9
1.2
1.6
4.2
7.0

1.2
1.7
3.9
6.9

1.3
1.7
3.8
6.8

1.2
1.8
3.8
6.8
1.3
1.7
3.7
6.7
Source: U.S. EPA, 2007e
* Because of number rounding, the sums of manure production do not always equal the reported totals.

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        Table 9: Estimate of fertilizer N used on turf grass in the United States in the year 2000,
                                  based on a total area of 12.6 million ha
Type of Turf Fertilized Area (Million ha) N rate (kg/ha/yr)* Total N Used (Tg/yr)
Nominal Fertilization
Professional Lawn Care
High Maintenance Areas
(golf/sports)
Total
4.7
0.93
1.26
6.89
73
296(195-488)
390
-
0.35
0.27
0.49
1.11
Source: Data derived from Milesi; et al., 2005 and A.M. Petronic, Personal Communication 2007
*A conversion factor of 1000 m2/ha was used with application rates (kg N/100 m2) of: 0.73 for nominal fertilization, 2.92
for professional lawn care, and 3.89 for high maintenance areas kg N/100 m2
on turf grass in the U.S. is 1.1 Tg N/year, or 9% of the
total average annual N-fertilizer used between 1999 and
2005. Depending on land use patterns, certain areas of the
country, particularly coastal areas where residential and
urban properties prevail, turf fertilizer can be  an important
or even dominant source of nitrogen to surface waters.
   Turf fertilizer N is susceptible to losses to the
atmosphere and surface and ground water when it is not
properly managed. Research on lawns has shown that
leaching of NO3~ can range between 0 and 50% of N
applied (Petrovic, 1990). Nitrogen leaching losses can
be greatly decreased by irrigating lightly and frequently,
using multiple and light applications of fertilizers,
fertilizing at the appropriate times (especially not too
late in the growing season), and using soil tests to ensure
proper balance of non-N soil condition and pH. In a soil
column experiment with turf coverage, the percentage
of N leached (as percentage of N applied) varied from
8% to 14% using light irrigation and from 2% to 37%
with heavy irrigation. Applying fertilizer in appropriate
amounts, avoiding periods when grass is dormant, and
not fertilizing too soon before irrigation or large rainfall
events can all help ensure leaching and runoff will be
minimal without affecting turfgrass color and growth
(Mangiafico and Guillard, 2006).
   Nitrogen runoff losses are poorly quantified but a range
similar to leaching is probable  (A.M. Petrovic, personal
communication). The chemical form of fertilizer N does
not impact leaching/runoff unless the fertilizer is applied in
late autumn (Petrovic, 2004a),  although use of slow release
or organic fertilizers can help reduce runoff and leaching.
Shuman (2002) notes that runoff can be limited by applying
minimum amounts of irrigation following fertilizer
application and avoiding application before intense rain or
when soil is wet. Losses of Nr to the atmosphere can be
significant when urea is applied. Measured denitrification
losses are usually small, but depend upon timing of N
application relative to soil water status, irrigation, and
temperature. Typically 25% of N applied is not accounted
for in runoff, leaching, and uptake/removal, or soil
sequestration (A.M. Petrovic, personal communication).
This suggests that volatilization and denitrification are
important loss vectors. Nitrogen volatilization (Beard and
Kenna, 2008) rates range from 0.9% under light irrigation
to 2.3% under heavy irrigation.
   While under-fertilization can lead to reduced grass
stand and weed encroachment which results in more
leaching and runoff N losses than from well managed
lawns (Petrovic and Larsson-Kovach, 1996; Petrovic,
2004b), Guillard (2008) recommends not fertilizing
lawns of acceptable appearance. Further, prudent
fertilization practices may include using one-third to
one-half (or less) of the recommended  application
rate (i.e.,  application rates below 0.5 kg/100m2) and
monitoring response (Guillard, 2008). Less or no
fertilizer may produce acceptable lawns, especially once
the lawn has matured, provided clippings are  returned
and mowing length is left high.
   As noted above, according to Petrovic (personal
communication) half the lawns in the U.S. may not
receive any fertilizer. Those lawns are presumably
satisfactory to their owners. Further N reductions can
be made if white clover is incorporated  into turf, and
grasses such as fescues (which require little or no N
supplements once mature) are selected for amenable
parts of the country. These practices can potentially
reduce N fertilization (and subsequent leaching risk) on
turf by one third or more, saving 0.4 or  more Tg  N/year.
When properly managed, turf grass provides a variety
of services that include decreasing runoff, sequestering
carbon dioxide, and providing a comfortable environment
in which to live (Beard and Green,  1994).
   Finding 7: Synthetic N fertilizer application to urban
gardens and lawns amounts to approximately 9% of the
total annual synthetic N fertilizer used in the United
States. Even though this N represents a  substantial portion
of total N fertilizer use, the efficiency with which it is
used receives relatively little attention.
   Recommendation 7a: To ensure that urban
fertilizer is used as efficiently as possible,  the Committee

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recommends that EPA work with other agencies such as
USDA as well as state and local extension organizations
to coordinate research to ensure that fertilization
recommendations are accurate and promote awareness of
the issue.
  Recommendation 7b: Through outreach and
education, supported by research,  improved turf
management practices should be promoted, including
improved fertilizer application and formulation
technologies and maintenance techniques that minimize
supplemental Nr needs and losses, use of alternative turf
varieties that require less fertilization, alternative ground
covers in place of turf, and use of naturalistic landscaping
that focuses on native species.

2.3. Nr Transfer and Transformations in
and  Between Environmental Systems
  This section discusses the transfers and flows of Nr
within and between environmental systems (ES), which
include atmospheric, terrestrial, and aquatic environments.
The first Section (2.3.1)  contains information on Nr
deposition from the atmosphere to terrestrial and aquatic
systems, presents estimates of input and recycling of
Nr within terrestrial systems, and discusses movement
of Nr from the terrestrial to the aquatic system. The
second Section (2.3.2) presents an estimate of storage of
Nr within the terrestrial system. Areas of uncertainty in
Nr transfer and transformation are discussed in Section
2.3.3. Section 2.3.3 also contains an example analysis of
Nr input and fate in 16 watersheds in the northeast U.S.
No comprehensive national data are available to assess
the transfer and transformations of Nr in and between
the atmosphere, terrestrial systems and aquatic systems.
The example analysis in Section 2.3.3 shows how an
evaluation of inputs and fate of Nr could be conducted for
a large watershed.

2.3.7. Input and Transfers ofNrin  the
United States
Nitrogen deposition  from the atmosphere to the
earth's surface
  Atmospheric input contributes substantially to the Nr
content of terrestrial and aquatic ecosystems for the U.S.,
but the magnitude and mechanisms of Nr deposition to the
earth's surface remain major unanswered environmental
questions. Along the eastern U.S. coast and eastern
Gulf of Mexico, atmospheric deposition of N currently
accounts for 10% to over 40% of new N loading to
estuaries (Paerl et al., 2002). Other watershed contribution
estimates range widely throughout the U.S., depending
on size of the watershed, the size of the estuary, and the
magnitude of contributing sources of atmospheric N
enrichment. Valigura et al. (2001) identified a median
atmospheric N contribution of about 15% for 42
watersheds located throughout the U.S.,  although the
maximum estimate was 60%.
   NOX, NH3 and their reaction products not deposited
onto continents are generally lofted into the free
troposphere where they can have a wide range
of influence and, in the case of NOX, because of
nonlinearities in the photochemistry, generate substantial
amounts of tropospheric ozone (U.S. EPA, 2006a).
Total N deposition involves both gases and particles,
and both dry and wet (in precipitation) processes. Rates
of deposition for a given species (in units of mass of N
per unit area per unit time) can be measured directly,
inferred from mass balance of the atmospheric budget,
or modeled numerically, but substantial uncertainties
remain with each of these techniques when applied
to deposition of any Nr species. A portion of the Nr
deposited to the earth's surface is re-emitted as NH3,
NO, or N2O (Galbally and Roy, 1978; Kim et al., 1994;
Civerolo and Dickerson, 1998; IPCC,  2007a; Crutzenet
al., 2008). Although  naturally-produced Nr is involved,
anthropogenic Nr dominates over most of the U.S. In
Appendix A we provide a review of the state of the
science concerning the total annual Nr deposition and
trends in that deposition to the contiguous 48 states.
   Deposition involves both oxidized and reduced N
species. Of the oxidized forms of atmospheric N, all the
members of the NOy family (NO, NO2, NO3, N2O5,
HONO, HNO3, NO3-, PAN and other organo-nitrates,
RONO2) can be transferred from the troposphere to the
surface, and some (e.g., NO) undergo bidirectional flux.
Volatile amines are also detected as NOy compounds
(Kashihira et al., 1982; Wyers et al., 1993). Although
a potent GHG, N2O is only emitted, not deposited
and therefore has not been considered in the material
presented here and in Appendix A. Of the reduced forms
of atmospheric nitrogen, NH3 and NH4+ play a major
role. There is also evidence of deposition of organic N
such as amino acids and isoprene nitrates, and recent
observations suggest that these can account for -10%
(possibly as much as 30%) of the U.S. NOX budget,
especially in summer (Keene et al., 2002; Horowitz et al.,
2007; Duce et al., 2008; Sommariva et al., 2008). While
this is a worthy research topic, measurements are still
limited and deposition of organic N compounds  has not
been reviewed in this report. The wide array of relevant
atmospheric compounds makes direct measurement and
accurate load quantification challenging. Appendix A
provides a discussion of wet and dry deposition  of Nr in
the U.S. and the relationship between emissions of Nr and
observed deposition.

Consideration  of NOy as a supplement for
the current NO2 National Ambient Air Quality
Standard
   The six principal (or criteria) pollutants for which
EPA has established National Ambient Air Quality
Standards (NAAQS) include "oxides of nitrogen" (the
sum of NO and NO2 ) or NOX. The specific chemical
compound nitrogen dioxide (NO2) has been selected as

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the indicator for compliance with the NAAQS for NOX.
The levels of primary and secondary standards forNO2
are identical at 0.053 ppm (approximately 100 ug/m3) in
annual arithmetic average, calculated from the one-hour
NO2 concentrations. In forming an integrated policy
for protecting the environment from adverse effects of
reactive nitrogen, it is appropriate to consider whether
the existing criteria pollutants are sufficiently inclusive of
Nr species. EPA's recent Integrated  Science Assessment
for Oxides of Nitrogen and Sulfur - Ecological Criteria
(U.S. EPA, 2008d) evaluated the scientific foundation for
the review of the secondary (welfare-based) NAAQS for
oxides of nitrogen and concluded that:
   The instrumentation deployed at present in the routine
   monitoring networks for determination of gas-phase
   NO2 and SO2 concentrations is likely adequate for
   determining compliance with the current NAAQS. But
   in application for determining environmental effects,
   all these methods have important limitations, which
   make them inadequate for fully characterizing the state
   of the atmosphere at present, correctly representing the
   complex heterogeneity ofN and S deposition across
   the landscape, and for realistically apportioning
   the contributions of reduced and oxidized forms of
   atmospheric N and S in driving observed biological
   effects at a national scale.
   Although the current standard for NO2  is inadequate to
protect welfare, there is a straightforward technical fix to
this problem. NOX (NO + NO2) is a variable, often small
component of reactive oxidized nitrogen (NOy) as has
been noted in EPA's Integrated Science Assessment for
Oxides of Nitrogen and Sulfur - Ecological Criteria and
extensively documented in the reviewed literature (Fahey
et al., 1986; Fehsenfeld et al.,  1987; Doddridge et al.,  1991;
Parrish et al., 1993; Poulida et al., 1994; Ridley et al.,
1994; Emmons et al., 1997; Liang et al., 1998; Munger et
al., 1998; Zhou et al., 2002; Takegawa et al., 2003; Parrish
et al., 2004a,b; Horii et al., 2004, 2006; Dunlea et al.,
2007; Kleinman et al., 2007; Hargrove and Zhang, 2008;
Luria et al., 2008; Zhang et al., 2008; Schwab et al., 2009;
Luke et al., 2010). The standard chemiluminescence NO/
NOX technique measures NO directly via reaction with
O3, and NOX (NO + NO2) by conversion of NO2 to NO
on hot molybdenum. It had been thought that NOX would
make up nearly all of NOy in heavily polluted urban areas,
but results including some from Mexico City (Dunlea et
al., 2007) indicate that this is not the case.  Interferences
were up to 50% of the ambient NO2  concentration even
in a heavily urban area. Recent results from Houston,  TX
(Luke et al., 2010) showed a median NOx/NOy ratio of
-0.63 between 1300 and  1500 local time, a time of rapid
ozone production. NO2 has been used historically  as the
indicator for many health studies, and it is regulated in part
because of studies that directly link it to respiratory effects.
However, given the inadequacy of NO2 as a standard to
protect welfare, the Committee recommends that EPA
reexamine the criteria pollutant oxides of nitrogen and the
indicator species NO2 and consider adding chemically
reactive nitrogen as a criteria pollutant, and NHX and NOy
as indicators to supplement the NO2 National Ambient Air
Quality Standard.
   The references listed above also document that current
suite of NOX monitors are useful for proving compliance
with the current NO2 standard, but suffer substantial
interferences and the extent of these interferences varies
with time and location. Reactive N compounds, especially
HNOs, can be lost on the inlet components. Because the
inlet does not transmit all reactive nitrogen compounds
with high efficiency, commercial NOX monitors may
provide an upper limit for NOX and a lower limit for
NOy, but they measure neither NOX nor NOy precisely.
Numerical simulations do not currently produce consistent
results for the partitioning of NOy species, for example
see Archibald et al., (2010). The data from the current
monitoring network are of limited value for determining
exposure to NO2, for evaluating chemical transport models
(CTMs), or for assessing efficacy of emissions control
strategies (McClenny et al., 2002).
   NOy monitors differ from the NOX monitors currently
in use in the position of the hot molybdenum NO2 to NO
converter and in calibration and operation (e.g., Thermo
Scientific Model 42i-Y). In an NOX monitor the converter
is well downstream of the inlet, behind a filter that
removes paniculate matter, while in an NOy monitor the
converter is at the inlet (Fehsenfeld et al., 1987; Doddridge
et al., 1991; Doddridge et al., 1992; Parrish et al., 1993;
Poulida et al., 1994; Ridley et al., 1994; Emmons et al.,
1997; Liang et al., 1998; Munger et al., 1998; Parrish et
al., 2004b; Dunlea et al., 2007; Hargrove and Zhang, 2008;
Luria et al., 2008; Schwab et al., 2009). Thus the current
monitors could be replaced or retrofitted to measure NOy
with a detection limit of 0.1 ppb at reasonable  expense
for equipment and for training operators. NOy monitors,
like NOX monitors, provide proof of compliance with
NO2 standards. For specific measurement of NO2,
commercial instruments for selective NO2 reduction (e.g.,
Air Quality Design, Inc., Wheat Ridge, CO) or direct
NO2 measurement are beginning to be available (Parrish
and Fehsenfeld, 2000; Castellanos et al., 2009) and these
may offer an alternative approach. Direct monitoring
of NO2 and other Nr species is a long-term goal, but
new techniques will require thorough testing in polluted
environments. NOy monitors using hot molybdenum
compare favorably with other NOy techniques such as
gold-catalyzed CO reduction and  several studies have
concluded that NOy can be reliably measured in suburban
and urban environments (Fehsenfeld et al., 1987;  Crosley,
1996; Williams etal., 1998).

Conclusions on atmospheric deposition of Nr
   As discussed here and in Appendix A, downward
transport from the atmosphere is a major source of
Nr to the earth's surface, but there are uncertainties in

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the characteristics and absolute magnitude of the flux.
Pollutants not deposited are exported from the continent
and alter the composition and radiative balance of the
atmosphere on a large scale. A review of the literature
revealed the following major points concerning the
present state of the science:
1. Measurements from the National Atmospheric
  Deposition Program (NADP) indicate that wet
  deposition of ammonium plus nitrate for the period
  2000-2006 averaged 3.1 kg N/ha/yr over the 48
  contiguous states.
2. The reduced (NH4+) and oxidized (NCV)  forms of
  reactive N contributed about equally to the flux, but
  input to the eastern United States was greater (and less
  uncertain) than to the western U.S.
3. For the U.S. east of the Mississippi River, dry
  deposition data have also been analyzed - the Clean Air
  Standards and Trends Network (CASTNET) monitors
  vapor phase HNOs, as well as paniculate NCV and
  NH4+. These measurements indicate 7.75  kg N/ha/
  yr total deposition (5.46 wet 2.29 dry) over the East.
  Conspicuous by its absence  from this number is dry
  deposition of ammonia.
4. Decreases in NOX emissions appear to have led to
  decreases in NCV deposition. NADP data show a
  national decreasing trend in the wet nitrate deposition
  and some individual sites show statistically significant
  decreases in deposition and correlations with emissions.
5. A thorough review of all published studies of the U.S.
  NOy budget indicates that about 70 % of the NOX
  emitted by the U.S. is deposited onto the continent
  with the remainder exported, although substantial
  uncertainty remains. Major sources of uncertainty
  concerning the deposition of NOy include dry
  deposition of unmonitored members of the NOy
  family, uncertainties in the chemistry of organic N, and
  poorly constrained estimates of convective venting of
  the planetary boundary layer of the atmosphere (i.e.,
  uncertainties concerning the venting of NOy from the
  lowest layer of the atmosphere).
6. Based on observations and model estimates of the
  relative deposition of unmeasured quantities, total
  estimated deposition of all forms of Nr for the period
  2000-2004 is ~11 kg N /ha /yr for the eastern U.S., and
  for the 48 states ~7.5 kg N /ha /yr with a range of 5.5 to
  9.5 kg N/ha/yr.
   Finding 8: Scientific  uncertainty about the origins,
transport, chemistry, sinks, and export of Nr remains high,
but evidence is strong that atmospheric deposition of Nr to
the earth's surface as well as emissions from the surface to
the atmosphere contribute substantially to environmental
and health problems. Nitrogen dioxide, NO2, is often a
small component of NOy, the total of oxidized nitrogen
in the atmosphere. The current NAAQS for NO2, as an
indicator of the criteria pollutant "oxides of nitrogen,"
is inadequate to protect health and welfare. NOy should
be considered seriously as a supplement or replacement
for the NO2 standard and in monitoring. Atmospheric
emissions and concentrations of Nr from agricultural
practices (primarily in the form of NH3) have not been well
monitored,  but NH4+ ion concentration and wet deposition
(as determined by NADP and NTN) appear to be
increasing,  suggesting that NH3 emissions are increasing.
Both wet and dry deposition contribute substantially to
NHX removal from the atmosphere, but only wet deposition
is known with much scientific certainty. Thus consideration
should be given to adding these chemically reduced and
organic forms of Nr to the list of Criteria Pollutants.
   Recommendation 8a: EPA should reexamine the
criteria pollutant "oxides of nitrogen " and the indicator
species NO2 and consider adding chemically reactive
nitrogen as a criteria pollutant, and NHX and NOy as
indicators to supplement the NO 2 National Ambient Air
Quality Standard.
   Recommendation 8b: Monitoring ofNHx and NOy
should begin as soon as possible to supplement the
existing network of NO 2 compliance monitors.
   Recommendation 8c: EPA should pursue the longer-
term goal of monitoring individual components ofNr, such
as NO2 (with specificity), NO and PAN, andHNO^, and
other inorganic and reduced forms, as well as support the
development of new measurement and monitoring methods.
   Recommendation 8d: The scope and spatial coverage
of the Nr concentration and flux monitoring networks
(such as the National Atmospheric Deposition Program
and the Clean Air Status and Trends Network) should be
increased and an oversight review panel for these two
networks should be appointed.
   Recommendation 8e: EPA in coordination with other
federal agencies should pursue research goals including:
   • Measurements of deposition directly both at the
     CASTNET sites and in nearby locations with non-
     uniform surfaces such as forest edges.
   • Improved measurements and models of convective
     venting of the planetary boundary layer and of long
     range transport.
   • Improved analytical techniques and observations
     of atmospheric organic N compounds in vapor,
     particulate, and aqueous phases.
   • Increased quality and spatial coverage of
     measurements of the NH^ flux to the atmosphere
    from major sources especially agricultural practices.
   • Improved measurement techniques for, and numerical
     models ofNOy andNHx species (especially with
     regard to chemical transformations, surface
     deposition and offshore export, and linked ocean-
     land-atmosphere models ofNr).

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Input and recycling of Nr within terrestrial
systems in the United States
   Annual input of newly created Nr onto terrestrial
ecosystems comes primarily from atmospheric
deposition, synthetic fertilizer and BNF in managed
and unmanaged ecosystems (Table  1). Although Nr
deposited from the atmosphere to terrestrial systems is
formed inadvertently during fossil fuel combustion and
from volatilization of NH3 from agricultural activities,
it serves to provide nutrients, along with biological
N fixation and synthetic fertilizer, for food, feed, and
fiber production in the agricultural  sector. Forests and
grasslands use Nr for growth. Home gardens, parks and
recreational areas utilize Nr within the urban landscape.
Approximately 32 Tg of new Nr reached the land of the
48 contiguous states in 2002 (Table 1). An additional
~0.2 Tg of N was imported mainly  as food and drink
products (FAO, 2007). An additional -12 Tg of Nr
was  recycled back to terrestrial and aquatic systems in
livestock excreta (-6 Tg N), human excreta  (-2 Tg N),
and crop residue from the previous year's production (-4
Tg N; U.S. EPA, 2007e). Of this N, - 1.3 Tg (-1.2 from
livestock manure and <0.1 from sewage sludge) was
used as fertilizer for crop production (U.S. EPA, 2007e).
More detailed information describing sources and
cycling of reactive nitrogen input in terrestrial systems is
provided in Appendix B.
   Finding 9: Total N budgets within all terrestrial
systems are highly uncertain. The relative amount of
N losses from terrestrial systems ascribed to leaching,
runoff, and denitrification are as uncertain as the N
budgets themselves.
   Recommendation 9: EPA should join with USDA,
DOE, and universities to work together in efforts to
ensure that the N budgets of terrestrial systems are
properly quantified and that the magnitudes of at least the
major loss vectors are known.

Transfer of Nr to aquatic systems
   Within the nitrogen cascade, Nr flows from the
atmosphere  and terrestrial systems into aquatic systems.
Aquatic systems include groundwater, wetlands,
streams and rivers, lakes and the coastal marine
environment. Nr is deposited directly into surface
aquatic systems from the atmosphere (direct deposition)
and Nr that is not either stored or removed as products
on terrestrial systems eventually moves into aquatic
systems (indirect deposition).
   The area of an airshed generally greatly exceeds that
of a watershed for a specific estuary or coastal region.
For example, the airshed of the Baltic Sea includes
much of western and  central Europe (Asman, 1994;
Hov et al., 1994), while the airsheds of the two largest
U.S. estuarine ecosystems, the Chesapeake Bay and
Albemarle-Pamlico Sound, are 15 to over 30 times the
size of their watersheds (Dennis, 1997). Thus, the airshed
of one region may impact the watershed and receiving
waters of another, making eutrophication a regional-scale
management issue (Galloway and Cowling, 2002; Paerl et
al., 2002). Furthermore, atmospheric N inputs do not stop
at coastal margins. Along the North American Atlantic
continental shelf, atmospheric N inputs more than match
riverine inputs (Jaworski et al., 1997; Paerl et al., 2002),
underscoring the fact that N-driven marine eutrophication
may require regional or even global solutions. Even in
truly oceanic locations (e.g., Bermuda), North American
continental atmospheric N emissions (reduced and
oxidized N) are commonly detected and significant (Luke
and Dickerson, 1987; Prospero et al., 1996). Likewise,
islands in the North Pacific receive N deposition
originating in Asia (Prospero et al., 1989).
  Riverine and atmospheric "new" Nr inputs in the
North Atlantic Ocean basin are at least equal and may
exceed "new" Nr inputs from biological N2 fixation
(Howarth et al. 1996; Paerl and Whitall, 1999; Paerl et
al. 2002). Duce et al. (2008) estimate that up to one-
third of the oceans' external Nr supply enters through
atmospheric deposition. This deposition leads to an
estimated - 3% of new marine biological production and
increased oceanic N2O production. Schlesinger (2009)
estimated that global atmospheric transport of Nr from
land to sea accounts for the movement of almost one
third of the annual terrestrial Nr formation. Therefore,
our understanding of marine eutrophication dynamics,
and their management, needs to consider a range of scales
reflecting these inputs, including ecosystem, watershed,
regional and global levels.
  One example of shifting N inputs is the proliferation
of intensive livestock operations in coastal watersheds,
which has led to large increases and changes in chemical
composition of nitrogenous compounds discharged to
estuarine and coastal waters via runoff, groundwater, and
atmospheric deposition (Paerl, 1997; Howarth, 1998;
Galloway and Cowling, 2002). In general, coastal waters
under the influence of these operations are experiencing
increases in total N loading as well as a shift toward more
reduced N (NH4+, organic N) relative to oxidized N (NO3~
) (Galloway and Cowling, 2002; Howarth et al., 2002).
These increases, combined with increases in hypoxia and
anoxia in receiving waters, are leading to more NH4+-
rich conditions, which will favor those algal groups best
able to exploit this N form, including some harmful
algal bloom (HAB) taxa (Paerl and Whitall, 1999;
Paerl et al., 2007). Similarly, conversion of forest and
agricultural lands to urban lands can alter landscapes and
promote N loading to estuaries by increasing impervious
pathways and removing natural landscape filters for Nr.
Development also destroys wetlands, leading to more
NO3'-enriched conditions, potentially favoring plant taxa
best able to exploit this N form.
  A recent evaluation of decadal-scale changes of
NO3"  concentrations in ground water supplies indicates

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                                   Box 1: Hypoxia in the Gulf of Mexico
     An example of a problem of excess Nr that moves from one part of the U.S. to another is the movement of Nr
  from the states that make up the Mississippi River drainage to the Gulf of Mexico (see discussion in Section 5.3.4).
  Ahypoxic zone covers a significant area of the receiving bottom waters of the continental shelf of the northern
  Gulf of Mexico (details may be gleaned from U.S. EPA SAB, 2007). This is a seasonally severe problem that has
  persisted there for at least the past 20 years.  Between 1993 and 1999 the hypoxia zone ranged in extent from  13,000
  to 20,000 km2 (Rabalais et al. 1996, 1999; Rabalais and Turner, 2001). The hypoxia is most widespread, persistent,
  and severe in June, July, and August, although its extent and timing can vary, in part because of the amplitude and
  timing of flow and subsequent nutrient loading from the Mississippi River Basin. The waters that discharge to the
  Gulf of Mexico originate in the watersheds of the Mississippi, Ohio, and Missouri Rivers (collectively described
  here as the Mississippi River Basin). With a total watershed of 3 million km2, this basin encompasses about 40% of
  the territory of the lower 48 states and accounts for 90% of the freshwater inflow to the Gulf of Mexico (Rabalais et
  al. 1996; Mitsch et al., 2001; U.S. EPA SAB, 2007).
     The December, 2007 report, Hypoxia in the Northern Gulf of Mexico: An update by the EPA Science Advisory
  Board (U.S. EPA SAB, 2007) determined that, "To reduce the size of the hypoxic zone and improve water quality
  in the Basin, the SAB Panel recommends a dual nutrient strategy targeting at least a 45% reduction in riverine total
  nitrogen flux (to approximately 870,000  metric tons/yr) and at least a 45% reduction in riverine total phosphorus
  flux (to approximately 75,000 metric tons/yr). Both of these reductions refer to changes measured against average
  flux over the 1980-1996 time period.  For both nutrients, incremental annual reductions will be needed to achieve the
  45% reduction goals over the long run. For nitrogen, the greatest emphasis should be placed on reducing spring flux,
  the time period most correlated with the  size of the hypoxic zone."
that there has been a significant increase in nitrate
concentrations in well water across the U.S. (Rupert,
2008). This study compared the nitrate content of 495
wells during 1988-1995 with nitrate content found during
2000-2004 as a part of the U.S. Geological Survey,
National Water Quality Assessment Program. In a subset
of wells, ground water recharge was correlated with
historic fertilizer use and it was concluded that nitrate
concentrations in ground water increased in response to
the increase of N fertilizer use.

2.3.2. Storage of Nr with in Terrestrial
Environmental Systems
  According to the nitrogen cascade conceptualization,
terrestrial environmental systems are compartmentalized
into agriculture, populated, and vegetated systems.
Annual input of Nr is greatest in agricultural ecosystems
(farmland, cropland, and grazed pastureland). Annual Nr
inputs to agricultural ecosystems, using 2002 as the base
year, include 9.8 Tg from synthetic fertilizer, 7.7 Tg from
biological N fixation in crops (mainly soybeans), and 1.3
Tg from atmospheric deposition. Nr input into vegetated
systems (mostly forested, but including non-cropland
grasslands and other natural vegetation types as well) comes
mostly from atmospheric deposition (3.2 Tg). Annual input
of Nr into populated systems includes synthetic fertilizer
application to urban turfgrass and recreational areas (~1.1
Tg), and atmospheric deposition 0.2 Tg.
  Much of the annual Nr input into these terrestrial
systems passes through, and is transferred within,
terrestrial systems or atmosphere via NH3, NOX or N2O,
or aquatic environmental systems via NCV and organic N
leaching and runoff or NHX and NOy deposition.
  The largest single reservoir of total N in the terrestrial
environmental system is soil organic matter (SOM).
Approximately 52,000 Tg C and 4,300 Tg N are contained
in the upper 100 cm of soil in the 48 contiguous states (N
is estimated from assumed C/N ratio of 12) (Lai et al.,
1998). For comparison, the total above ground biomass
of U.S. forests of these states contains ~ 15,300 Tg of C
and ~ 59 Tg N (estimated using a C/N ratio of 261), and
15,500 Tg of soil organic matter carbon (SOM-C), and
1,290 Tg total N (estimated using a C/N ratio of 12) (U.S.
EPA, 2007e). Most of this soil organic matter nitrogen
(SOM-N) is bound within complex organic molecules
that remain in the soil for tens to thousands of years. A
small fraction of this SOM is mineralized, converted to
carbon dioxide and Nr annually.  The total N contained
within above and below ground compartments isn't really
of concern.  What is of interest in addressing issues of
Nr, is the change in N stored within the compartments
of terrestrial systems. The pertinent question is whether
N is being retained or released from long-term storage.
The Committee evaluated estimates of annual change
of N storage within important components of terrestrial
systems. The Committee used Carbon stock information
in Chapter 7 of the EPA Inventory of U. S. Greenhouse
Gas Emissions and Sinks 1990-2005 (U.S. EPA, 2007e)
to estimate N storage in U.S. terrestrial systems. Nitrogen
stock change was determined by simply assigning a molar
C/N ratio of 12 for soils and 261 for trees and making the
appropriate conversions from C to N.

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Agricultural systems
   Croplands within the contiguous 48 states occupy
~ 368 million acres (149 million ha) (19%) of the 785
million ha of total land area. In 2002, 126 million ha
of this cropland were cultivated (USDANRCS, 2007).
Croplands are generally found on well drained mineral
soils (organic C content 1-6% in the top 30 cm). Small
areas of drained organic soils are cultivated (organic C
content of 10-20%) in mainly Florida, Michigan and
Minnesota (EPA, 2007e). Organic soils lost -0.69 Tg of
Nr in 2002 while mineral soils accumulated ~1.5 Tg of Nr
(Table 10). Much of the accumulation of SOM-C was due
to the use of conservation tillage and high yielding crop
varieties (U.S. EPA, 2007e). Losses of Nr from organic
soils are due to mineralization of SOM and release of
Nr input. In cultivated soils annual input of new Nr is
approximately 9.7 Tg from fertilizer N, 1.1 Tg from
livestock manure (recycled N), -7.7 Tg from biological
N fixation, and 1.2 Tg from atmospheric deposition.
Assuming that loss of fertilizer N from the small area of
organic soils is a minor fraction of the total, -17% of N
input from synthetic fertilizer, -12% of total N input, is
stored in cropland mineral soils annually.
   According to the U.S. EPA National Greenhouse
Inventory (U.S. EPA, 2007g) the net increase in soil
C stocks over the period from 1990 through 2005 was
largely due to an increase in annual cropland enrolled in
the Conservation Reserve Program, intensification of crop
production by limiting the use of bare-summer fallow in
semi-arid regions, increased hay production, and adoption
of conservation tillage (i.e., reduced- and no-till practices).
It is clear that conversion of marginal crop land to CRP
stores C and reduces erosion and nitrate leaching. Likewise,
use of soil conservation tillage, as opposed to conventional
tillage with a plow or disk, reduces erosion. However,
the impact of conservation tillage on soil C storage and
Nr losses due to leaching or denitrification are much less
certain. For example, although the EPA estimates shown
in Table 10 assume that no-till crop production results
in net carbon sequestration, recent publications indicate
that no-till cropping practices do not result in net carbon
sequestration (Verma et al., 2005; Baker et al. 2007;
Blanco-Canqui and Lai, 2008). Therefore, the estimates of
soil C and N storage in mineral soils in Table 10 that were
derived from U.S.EPA, (2007b) need to be reconsidered.
These new studies and that of David et al. (2009) suggest
that organic C conservation by reduced tillage practices has
been overestimated because soil sampling and analysis has
been confined to the top 30 cm of soil when the top meter
of soil needs to be considered. Baker et al. (2007) and
Verma et al. (2005) also show that long-term, continuous
gas exchange measurements have not detected C gain due
to no-till practices. They concluded that although there are
other good reasons to use no-till, evidence that it promotes
C sequestration is not compelling. These findings highlight
the need for appropriate assessment of ecosystem N storage
in order to confirm or disprove this Committee's conclusion
that only a small part of annual Nr input is stored in
agricultural lands, forests, and grasslands.

Populated systems - urban lands
   Populated or "developed land" (developed land is the
terminology used by the U.S Department of Agriculture's
Natural Resources Conservation Service [NRCS]) occupied
—106 million acres (42.9 million ha) of the U.S. land area
in 2002. This equates to approximately 5.5% of the U.S.
land area (USDANRCS, 2007). The EPA Inventory of U.S.
Greenhouse Gas Emissions and Sinks (U.S. Environmental
Protection Agency, 2007e) indicates that urban areas cover
over 4.4% of the land area with tree canopy covering
27.1% of the urban area. The tree-covered area constitutes
approximately 3% of total tree cover in the continental U.S.
If the NRCS value of 42.9 million ha is used, then trees
cover—27.9 million acres (11.3 million ha) of urban land in
the contiguous 48 states. Another -35.1  million acres (14.2
million ha)  of land is covered by turf grass in parks, golf
courses, and lawns. In both urban forests and turf grass, Nr
storage is dependent upon the age of the  trees or turf. In
young (pre-steady state) systems N is being accumulated,
while at steady state  no net change occurs. Some areas may
be degrading and actually losing biomass and returning N
to the environment. EPA does not estimate carbon changes
in turf grass, but does estimate changes in carbon storage in
urban forests (U.S. EPA, 2007e). Urban  trees sequestered
an estimated net 22 Tg of carbon and 0.12 Tg of N in 2002
(assuming that urban trees are mainly hardwood having
a C/N ratio  of 186) (U.S. EPA, 2007e). Annual fertilizer
N input into the urban landscape is approximately 10%
of total fertilizer N consumption in the U.S. (U.S. EPA,
2007e), or -1 Tg of N in 2002. Another 0.2-1.0 Tg N is
deposited from the atmosphere. In some  areas deposition
can be disproportionately high due to locally high NOy
concentrations. Storage of-0.12 Tg N in urban forests
constituted  approximately 3% of Nr input annually.

Vegetated systems - forests and  grasslands
   Forests cover approximately 164 million ha, -21%
of the land area of the contiguous 48 states (USDA
NRCS, 2007). The forest carbon stocks analysis by
EPA (U.S EPA, 2007e) is based on state surveys that
are conducted  every 1 to 10 years. Annual  averages
are applied to years between surveys.  To determine
changes in C related to the rate  of tree growth, Birdsey
(1992) estimated that there were 52,500 Tg of C above
and below  ground in U.S. forests; soil contained 59%
of total C, 9% was in litter, and 5% in tree  roots. The
EPA estimate for 2002 was 43,600 Tg  of C. To estimate
N storage based on  EPA data (U.S. EPA, 2007e), the
Committee has assumed that forests are 85% softwood
and 15% hardwood with an average C/N ratio of 261.
These estimates indicate that forests and forest products
stored -0.43 Tg of N in 2002 (Table 10).

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   Grasslands, including rangelands and pasturelands,
occupy approximately 213 million ha (27.1%) of the
contiguous 48-state land area. The NRCS divides
these grasslands into pastureland (48.2 million ha) and
rangeland (164 million ha). Pastureland is managed (it
may be fertilized and mown) and rangeland is managed
only to the extent that livestock grazing intensity is
regulated on the land used for such grazing. Changes in
the N status of grasslands are dependent upon changes in
soil organic matter as the above ground biomass produced
annually is either consumed by livestock or decomposed
in the field.  Soil organic C stocks were estimated using
the Century biogeochemical model and data used were
based upon the NRCS/National Resources Inventory
(NRI) survey (U.S. EPA, 2007e). Changes in soil N
content were estimated using a C/N ratio of 12. Nitrogen
input into rangelands is generally only from atmospheric
deposition, which contributes 1.9 Tg N each year to range
production. Rangeland tends to be in relatively remote
areas where atmospheric Nr deposition is low.
   Collectively, forests and grasslands stored -0.74 Tg
of N in 2002. Much of the soil N storage in grasslands is
a result of conversion of croplands to grasslands, mainly
due to the conservation reserve program. Forest soils
      Table 10: Net annual change in continental U.S. croplands soil N and C, forest C and N, and
                                      grasslands C and N in 2002
   Cropland remaining cropland
         Mineral soil
          17
 1.4*
         Organic soil
         -8.3
 -0.69
   Land converted to cropland
         0.8
0.067
                         Total
   Forests
         9.5
 0.80
   Forests and harvested wood products
         Above ground biomass
         85
 0.32
         Below ground biomass
          16
 0.063
         Dead wood
         9.1
0.035
         Litter
         7.2
0.028
         Soil organic matter
         -2.8
 -0.23
         Harvested Wood
         59
 0.22
                         Total
         174
 0.44
   Grasslands
   Grasslands remaining grasslands
         Mineral soil
         -0.8
-0.067
         Organic soil
         -1.3
 -0.11
   Lands Converted to Grasslands
         5.8
 0.48
                         Total
   US Total C & N Storage in 200'
         3.7
 0.31
Measurements in Tg. Negative sign indicates a decrease in storage; positive number indicates increase in storage. Soil
C/N ratio = 12; wood C/N = 261. C storage numbers were obtained from U.S. EPA, 2007e.* See previous discussion of
soil organic matter accumulation in croplands.

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appear to be losing N while overall N storage in forests is
from accumulation in above-ground biomass and that that
remains in forest products that are stored for long periods.

Summary of estimates of Nr stored in terrestrial
systems in 2002
   Although our estimate of N storage in terrestrial
systems is highly uncertain, annual N storage is likely
relatively small. Approximately 1.5 Tg N was stored in
terrestrial systems of the contiguous 48 states in 2001
(Table 10).  Soils were the largest reservoir with croplands
(0.8) and grasslands (0.3) sequestering most of the N.
Annual storage in agricultural, grassland and forest
soil and in forest biomass is approximately 6 to 10% of
annual Nr input.  Estimated total Nr input from synthetic
fertilizer, biological N fixation, and atmospheric deposition
into terrestrial systems within the contiguous 48 states in
2002 was ~32 Tg. All of the input and outflow numbers
are highly uncertain, but N loss through denitrification
appears to be the major loss mechanism. Storage in soils
and trees appears to account for only a small portion of the
annual N input while apparent loss through denitrification
dominates the budget. This is discussed in Section 2.3.3 of
this report and in a recent global Nr review by Schlesinger
(2009). Some small fraction is re-volatilized and exported
from the continent.

2.3.3. Areas of Uncertainty in Nr Transfer
and Transformation
   In considering Nr transfers and transformations in
and between the environmental systems of the nitrogen
cascade, the Committee has encountered a number of
areas where quantities or flows of Nr are highly uncertain.
All of these areas need attention from EPA in conjunction
with other federal and state agencies and universities.
Although most of the following points have been
highlighted in various findings and recommendations
within other chapters of this report, we feel the need to
highlight the following areas:
  Total denitrification in animal feeding operations, in
  soils, and in aquatic systems needs to be quantified
  along with all gaseous products produced and released
  to the atmosphere during nitrification/denitrification.
  These gases include NOx, N2O and N2.
  The amount of Nr transferred to each environmental
  system as dry deposition needs to be quantified and
  monitored.
  The fraction of NOy in the form of organo-nitrates  and
  other organic nitrogen species is poorly quantified, but
  may play a major role in air quality and Nr cycling.
  Rates and amounts of ammonia emissions from
  fertilized soils and animal feeding operations need to be
  quantified and the fate of this ammonia determined.
  The annual change in N  storage in soils (agricultural,
  forest, grassland and urban areas) needs to be quantified
  in conjunction with the change in carbon.
   These areas of high uncertainty are highlighted
because very little information exists in some of the areas.
In other areas, such as denitrification and the relative
release of N2O from soils and aquatic systems, the sparse
data are highly variable and this makes developing
meaningful guidelines for control difficult. An analysis of
Nr input and fate in 16 watersheds in the U.S. is provided
as an example to illustrate how the inputs and fate of Nr
can be evaluated for a large watershed.

Input and fate of Nr in 16 watersheds in the
northeast United States
   There are no comprehensive data available to assess
the transfer and transformations in and between the
atmosphere, terrestrial systems (agriculture, populated,
and vegetated systems) and aquatic systems nationally.
Determining a national N budget is a priority research
area. As there are no national data available, an example
analysis of Nr input and fate in 16 watersheds in the
northeast U.S. (for which data are available) is  used to
show an evaluation of the inputs and fate of Nr for a large
watershed (Van Breemen et al., 2002).
   The watersheds in this study encompass a range of
climatic variability, Maine to Virginia. The watersheds are
a major drainage to the coast of the North Atlantic Ocean.
Using data from the early 1990s, Boyer et al. (2002)
reported the quantification of N inputs to each watershed
from atmospheric deposition, N fertilizers, biological N
fixation, and import of N in agricultural products (food
and feed). They compared inputs with N losses from the
system in riverine export. As a part of the same study,
Van Breemen et al. (2002) analyzed the fate of N inputs
to these watersheds and developed budgets for  each
watershed. The  total area of the watersheds was 32,666
square km with land use categories of forest (72%),
agricultural (19%), urban (3%), wetlands (5%), and
1% other uses. The Nr input into the watersheds (using
weighted averages for all 16 watersheds) was 3,420 kg
per square km per year. Figure 14 shows the Nr sources
and the estimated fate of this Nr as a per cent of the
weighted average Nr input.
   Van Breemen et al. (2002) indicate that Nr inputs and
storages and losses were well correlated (R2 = 0.98).
Denitrification in landscape soils is the most uncertain
estimate because rates are calculated by difference
between total inputs and outputs so they accumulate
errors from all estimates. The denitrification loss term
may also reflect the change in N storage in groundwater.
The net storage of N in the soil (18% of total storage and
losses) indicates that there is a non-steady state condition
in the soil. Increasing storage of Nr on land implies that
drainage and denitrification exports of Nr are likely to
increase when a new steady-state condition is reached.
   These data suggest that Nr research needs to focus on
understanding the "denitrification" loss term in this analysis.
The losses occur in the terrestrial landscape, before Nr

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                 Nr Sources
                   Forest Fixation
                       5%
   Net Import in
   Food & Feed
      24%
                Atmospheric
                 Deposition
                   33%
      Agricultural Fixation
            23%
            Nr Storage &  Loss
                        Ammonia
                       Volatilization
                                 Dentrification
 Denitrification
 in Landscape
    37%
        Soil Storage
          Increase
            9%
          Food
Biomass    Export
Increase     6%
  9%
                 Riverine Export
                     20%
                                     Wood Export
                                         5%
enters the river. Where do these losses occur, within the
agricultural field, in drains and ditches near the agricultural
field, in riparian areas, or wetlands? Understanding this
term may help in the management of Nr in watersheds to
decrease nitrate movement into aquatic systems as well as to
limit N2O emissions to the atmosphere.
   The Van Breemen et al. (2002) study also estimated
that approximately 30% of N input was exported to the
rivers and about two thirds (20% of total N input) of this
N was exported to coastal waters by rivers. The remaining
one-third (11% of total N input) was considered to
have been denitrified in the rivers. These examples
also demonstrate that Nr in the atmosphere, terrestrial
systems and aquatic systems are not separate and must
be considered collectively. Atmospheric deposition is
a variable but important input into aquatic systems that
contributes to Nr enrichment problems. Aquatic and
terrestrial systems process this Nr and return other Nr
gases  (NH3, NOX and N2O to the atmosphere). Nr from
terrestrial systems impacts both the atmosphere and
aquatic systems through emission of NH3, NOX, N2O and
leaching and runoff of NOs".
   Finding 10: Denitrification of Nr in terrestrial and
aquatic systems is one of the most uncertain parts of the
nitrogen cycle. Denitrification is generally considered
to be a dominant N loss pathway in both terrestrial and
aquatic systems, but it is poorly quantified.
   Recommendation 10:  EPA,  USDA, DOE, and
universities should work together to ensure that
denitrification in soils and aquatic systems is properly
quantified, by funding appropriate research.
Figure 14: Nr input and loss from 16 watersheds
in the northeast United States
Source: Van Breeman et al., 2002 (Figure 7, p. 289).
Reprinted with permission; copyright 2002,  Springer
Science+Business Media B.V.

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 Impacts  of  Nr on Aquatic, Atmospheric,
 and  Terrestrial  Ecosystems
   This chapter summarizes knowledge of the impacts
of Nr on freshwater, coastal, atmospheric, and terrestrial
ecosystems. Table ES-1 in the Executive Summary
provides a more detailed presentation of the quantitative
extent of Nr impacts on the environment.

3.1. Impacts on  Drinking Water,  Human
Health, and Freshwater Biota
   A detailed presentation on the impacts of Nr on
various aquatic systems is presented in Appendix G. The
EPA's Office of Water (U.S. EPA, 2007b) has noted the
following impacts caused by excessive Nr in aquatic
systems:
  Excessive nutrients (N and P) can cause negative
  ecological impacts to water bodies on a national scale
  by stimulating harmful algal blooms.
   • Algal blooms block sunlight and result in the
    destruction of submerged aquatic vegetation which
    serves as critically important habitat and food for
    many organisms.
   • Algal blooms eventually die off and consume
    dissolved oxygen from the water column which can
    lead to die off of aquatic organisms.
   • One result of algal blooms is decreased biological
    diversity and populations, including smaller
    populations of game and commercial fish.
   • Some blooms, considered harmful algal blooms or
    HABs, have a toxic effect on living organisms and
    are disruptive of ecosystem structure and transfer of
    energy to higher trophic levels.
  Excessive nutrients also pose public health risks.
   • Algal blooms can cause taste and odor problems in
    drinking water.
   • Hazardous algal blooms can cause respiratory distress
    and neurological problems in swimmers.
   • Excessive nitrates can cause "blue baby syndrome."
  Nutrient pollution is occurring at a national scale and
  has not been completely addressed.
   • 49 states and 4 territories have 303(d) listings due to
    nutrients, and about 50% of the  states have greater
    than 100 water quality impairments due to nutrients.
   • Over 10,000 impairments are a result of nutrient
    pollution.
   Finding 11: There is growing recognition of
eutrophication as a serious problem in aquatic systems
(NRC, 2000). The last comprehensive National Coastal
Condition Report was published in 2004 (U.S. EPA,
2004) and included an overall rating of "fair" for
estuaries, including the Great Lakes, based on evaluation
of more than 2,000 sites. The water quality index, which
incorporates nutrient effects primarily as chlorophyll-a
and dissolved oxygen impacts, was also rated "fair"
nationally. Forty percent of the sites were rated "good"
for overall water quality, while 11% were "poor" and
49% "fair."
   Recommendation 11. The Committee recommends
that EPA develop a uniform assessment and management
framework that considers the effects ofNr loading
over a range of scales reflecting ecosystem, watershed,
and regional levels. The framework should include all
inputs related to atmospheric and riverine delivery
ofNr to estuaries, their comprehensive effects on
marine eutrophication dynamics and their potential for
management.
   Finding 12: Meeting Nr management goals for
estuaries, when a balance should be struck between
economic, societal, and environmental needs, seems
unlikely under current federal law. Enforceable authorities
over nonpoint source, stormwater, air (in terms of critical
loads), and land use are not adequate to support necessary
Nr controls. Funding programs are presently inadequate to
meet existing pollution control needs. Furthermore, new
technologies  and management approaches are required
to meet ambitious Nr control needs aimed at restoring
national water quality.
   Recommendation 12. The Committee recommends  that
EPA reevaluate water quality management approaches,
tools, and authorities to ensure Nr management goals
are attainable, enforceable, and the most cost-effective
available. Monitoring and research programs should
be adapted as necessary to ensure they are responsive
to problem definition and resolution, particularly in
the development and enhancement of nitrogen removal
technologies and best management practices, and continue
to build our level of under standing and increase our ability
to meet management goals.

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3.2. Impacts of Airborne or Atmospheri-
cally Deposited  Nron Human Health and
Ecosystems
   Six major atmospheric effects are associated with
increased NOX and NH3 emissions, and two with N2O
emissions (Galloway et al., 2003). For NOX and NH3
emissions, the effects are:
  Decreases in atmospheric visibility caused by fine PM
  Elevated ozone concentrations that enhance the
  greenhouse potential of the atmosphere
  Serious ozone and fine paniculate matter impacts on
  human health (Pope et al., 1995; Pope, 2000a,b ; Brook
  et al., 2003; Brunekreef et al., 2005; Pope, 2009)
  The important role that NH3 plays in the direct and
  indirect effects of aerosols on radiative forcing and
  thus on global climate change (Penner et al., 1991;
  Seinfeld and Pandis, 1998; Lelieveld et al., 2001;
  Myhre, 2009)
  Decreased productivity of crops, forests, and natural
  ecosystems caused by ozone deposition
  Atmospheric deposition of NH, NH3, NOy, and
  organic forms of Nr that can contribute to ecosystem
  acidification, fertilization, and eutrophication
   For N2O, the effects are the greenhouse effect in the
troposphere and O3 depletion in the stratosphere.

3.3. Impacts of Nr on Terrestrial
Ecosystems
   As previously discussed,  in many terrestrial ecosystems
the supply of biologically available Nr is a key factor
controlling the nature and diversity of plant life, and vital
ecological processes such as plant productivity and the
cycling of carbon and soil minerals. Human activities
have not only increased the supply but enhanced the
global movement of various forms of nitrogen through air
and water. Appendix H presents information on the impact
of Nr saturation on ecosystem function.
   The primary source of excess Nr for most unmanaged
terrestrial ecosystems is atmospheric deposition. This
additional Nr causes a  wide  variety of sometimes
beneficial effects (increased growth and productivity of
forests, natural grasslands, and crops planted in nutrient
deficient soils) and also sometimes adverse effects on
terrestrial and aquatic ecosystems in many parts of the
U.S. Forests and grasslands  exposed to excess Nr can
respond in numerous ways. General effects include the
following (Woodman and Cowling,  1987; Cowling, 1989;
Garner et al., 1989; Cowling et al., 1990; Vitousek et al.,
1997a,b; Cowling et al., 2002):
  Increased productivity of forests soils, most of which
  are Nr-limited throughout the U.S. Nr deficiency  of
  forest soils has been most fully quantified for pine
  forests in 14 southeastern states.
Acidification of forest soils leading to decreased
availability of nutrient cations including calcium,
magnesium, and potassium and aluminum toxicity,
established most clearly in the eastern U.S. and both
central and northern Europe.
Nr saturation of forest soils (which results in increased
Nr release to the water draining the soils), presently
occurring mainly in high-elevation forests of the
eastern U.S. and southeastern Canada.
Ozone-induced predisposition of forest trees to damage
by fungal diseases and insect pests, most clearly
established in the case of root disease and bark beetles
in the pine forests of southern California.
Ozone-induced inhibition of photosynthesis in both
softwood and hardwood tree species most clearly
established in controlled exposure studies in both the
U.S. and Europe at ambient concentrations of ozone
above 60 ppb.  Such concentrations occur frequently
throughout the eastern U.S. and southeastern Canada.
Ozone-induced direct injury to foliage,  most clearly
established in the case of "emergence tip burn" in
eastern white pine.
Acidification-induced decrease in frost hardiness of
high-elevation conifer forests, most clearly established
in the case of red spruce in the northeastern U.S.
Acidification-induced alteration of beneficial symbiotic
relationships in forest soils, especially mycorrhizae,
most clearly established in both northern and central
Europe.
Biodiversity losses in natural grasslands and forest
areas caused by Nr-induced decreases in abundance
of Nr-limited tree and grass species and replacement
by Nr-loving weed species, most clearly established in
both Minnesota and California,  and even more vividly
in The Netherlands.
Decreases invisibility and increased haziness  of the
atmosphere at scenic vistas in national and state parks
and wilderness areas.
More leaching of Nr to aquatic systems via both
groundwater and surface runoff—a cascade effect.

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Metrics  and  Implications  for  Risk Reduction
Strategies for  Reactive  Nitrogen
   It is important to develop risk reduction strategies
for reactive nitrogen that take into consideration the
ways in which Nr is introduced and transformed in
the environment. This chapter reviews current and
historical measurement and risk reduction activities
for Nr and provides specific Committee findings and
recommendations.

4.1. Measurement of Nr in
the Environment
   Although nitrogen is among the most abundant
elements on earth, only a small fraction, Nr, is responsible
for impacts on the environment. Most regulations focus
narrowly on specific chemical forms of nitrogen as they
affect media- or site-specific problems, setting limits or
specifying control technologies without regard to the
ways in which N is transformed once introduced into
the environment. Measurement methods are typically
expressed in terms of mass loadings or concentrations of
a particular form of N (e.g., ppm NOX, mg/L total NHX, or
kg/ha of NO3-).
   Finding 13: The Committee finds that there is a need
to measure, compute, and report the total amount of Nr
present in impacted systems in appropriate units. What
is measured influences what we are able to perceive and
respond to; in the case of Nr, it is especially critical to
measure total amounts and different chemical forms, at
regular intervals over time.
   Recommendation 13: The Committee recommends
that EPA routinely and consistently account for the
presence ofNr in the environment informs appropriate
to the medium in which they occur (air, land, and
water) and that accounting documents be produced and
published periodically (for example, in a fashion similar
to National Atmospheric Deposition Program [NADP]
summary reports). The Committee understands that
such an undertaking will require substantial resources,
and encourages the Agency to develop and strengthen
partnerships with appropriate federal and state agencies
and private-sector organizations having parallel interests in
advancing the necessary underlying science ofNr creation,
transport and transformation, impacts, and management.

4.2. Consideration of Nr Impacts  in  Risk
Reduction Strategies
Historical measurement and impact categories
   The types of impacts ofNr in the environment are
dependent on three general factors: the sources ofNr, the
types of media impacted, and Nr chemical forms. The
magnitude of effects depends on loading and the nature
of the system impacted. As illustrated in Figure 1, the
impacts of a given source of Nr can be multiple as N is
transformed in the environment and transported among
ecosystem components. The nitrogen cascade provides a
comprehensive framework for understanding the role of
Nr in the earth's ecosystems and establishes a framework
for developing and implementing management methods
through which beneficial effects can be enhanced while
minimizing detrimental impacts.
  A management paradigm in which various approaches
are used to limit environmental impacts to "acceptable"
levels of risk is a useful concept for understanding the
environmental impacts that Nr can have. For this purpose,
impacts are divided into several general categories
within which various contaminants have a direct
correlation with damage. Once the nature and type of
impacts are recognized, the risks should be characterized
quantitatively, if possible. This information would then be
used, along with other considerations such as economic,
social and legal factors, to reach decisions regarding risk
reduction strategies and the need for and practicability
of implementing various risk reduction activities. The
regulation of Nr in the environment by EPA follows an
impact-by-impact approach which, with few exceptions,
examines specific N forms  in either aquatic, atmospheric,
or terrestrial systems. As previously discussed, the
principal regulatory authority pertaining to nitrogen is
derived from the CWA and the CAA, although other
legislation such as the Energy Independence and Security
Act (EISA), and the Endangered Species Act (ESA)
contain provisions that could result in regulatory actions
that affect nitrogen management.
  Historically, EPA environmental protection programs
have addressed impacts ofNr such as climate change,
eutrophication, ecotoxicity, human health (cancer
and non-cancer), acidification, smog formation, and
stratospheric ozone depletion, among others (Bare et al.,
2003). Within these categories it is sometimes  possible
to express end points in terms of collective metrics, such
as is done with greenhouse gases in the form of carbon
dioxide equivalents, or acidification as H+ equivalents.
This approach has the considerable advantage  of defining
a straightforward framework within which environmental
standards can be derived that are protective of human
health and the environment - EPA's principal mission.
This approach also encourages evaluation of damage

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from multiple sources, as long as the characterization
metric used is genuinely representative of the impact of
a given contaminant. Thus, for example, the total impact
of acidic gases such as 862 and NOX on the acidification
of watersheds can be expressed as a common metric.
However, metrics for human health are generally not as
simple to characterize nor are the appropriate end points;
thus, the mechanism of toxicity, number of individuals
affected, value of lost workdays, medical treatment costs,
and value of human lives lost may all be used.

Ecosystem functions and services
  A complementary approach to classical impact
characterizations is the use of ecosystem "service" and
"function" categories, in which the impairment of a specific
service provided by one or more ecosystems or impairment
of an ecological function by causative contaminant
emissions is assessed (Costanza, 1997; Millennium
Ecosystem Assessment, 2003). Such an approach is
inherently attractive because of its basis in scientific reality,
i.e., the health of humans is inextricably linked to the health
of the environment. Less clear, in some cases, are ways in
which to measure and monitor such impacts and account
for the effects of a complex array of factors and stressors
that contribute to or damage ecosystem service, function,
and health. Table 11 provides examples of ecosystem
services and corresponding functions.
  The use of ecosystem services in a regulatory context
would be a different approach for the EPA, one with
considerable potential, but one for which experience is
currently lacking. In comparison to the available data on
reactive  nitrogen usage, little is known about the response
of ecosystems and ecosystem services to reactive nitrogen
loads. This is discussed more fully in Sections 4.5, 4.6 and
Appendix D on critical loads. In this context the Committee
                  Table 11: Ecosystem service and corresponding function categories

tcosysiem service
Gas regulation
Climate regulation
Disturbance regulation
Water regulation
Water supply
Erosion control and sediment retention
Soil formation
Nutrient cycling
Waste treatment
Pollination
Biological control
Refugia
Food production
Raw materials
Genetic resources
Recreation
Cultural
•••••••••••H

Regulation of atmospheric chemical composition
Regulation of global temperature, precipitation, and other
biologically mediated climatic processes at global, regional,
and local levels
Capacitance, damping, and integrity of ecosystem response
to environmental fluctuations
Regulation of hydrologic flows
Storage and retention of water
Retention of soil within an ecosystem
Soil formation processes
Storage, internal cycling, processing, and acquisition of
nutrients
Recovery of mobile nutrients, and removal or breakdown of
toxic compounds
Movement of floral gametes
Trophic dynamic regulation of populations
Habitat for resident and transient populations
That portion of gross primary production extractable as food
That portion of gross primary production extractable as raw
materials
Sources of unique biological materials and products
Providing opportunities for recreational activities
Providing opportunities for noncommercial uses
Source: Costanza et al., 1997 (Table 1, p. 254). Reprinted with permission; copyright 1997, Nature Publishing Group.

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supports plans by the EPA to incorporate research on
the services concept, focusing on Nr as the suite of
contaminants of interest, into its future ecological research
plan (U.S. EPA, 2009a). EPA's Ecological Research Plan
was reviewed by the Science Advisory Board (U.S. EPA
SAB, 2008). More recently, the Science Advisory Board
completed a serf-initiated study on "Valuing the Protection
of Ecological Systems and Services" (U.S. EPA SAB,
2009). This report explores the concept of ecosystem
services as a basis for regulatory action and presents a
roadmap for implementing this approach.

Economic measures and impacts
   It is also possible to translate the effects of Nr into
economic terms. Two economic measures that are often
used are the dollar costs of damages and the cost of
remediation or substitution. Another important economic
metric is the cost/ton of remediation for each form of Nr.
Damage costs do not always scale as tons of Nr released
into the environment. If damage costs rather than tons of
nitrogen were utilized as a metric, the full implications of
the cascade and the setting of priorities for intervention
might differ.
   It is important to note that the choice of metric used in
assessing impacts may play an influential role in what and
how one manages. Air and water protection laws state that
the goal is "to protect human health and the environment."
Yet, there is no generally agreed-upon common metric for
measuring the full range of effects (which are complex
and often unknown) or for setting priorities in the
establishment or implementation of policies.
   As noted above, there are multiple metrics for
measuring Nr or any other agent in the environment. The
most common metric utilizes quantitative measures of
the total amount of Nr (and any of its specific chemical
forms)  in different environmental reservoirs and the
mass flux between them. But while providing common
units, typically mass or concentration, these measures
do not distinguish the relative societal costs of health
or environmental consequences of reactive nitrogen of
different forms or places in the cascade. While not all
damages can be turned into economic costs, and the costs
of some damages have not been quantified, enough of the
major damages can be quantified economically to provide
a useful complementary metric for decision-making. (See
the Chesapeake Bay example in Box 2.)
   The advantage of monetizing damages is that it reflects
an integrated value that human society places on lost
ecosystem goods and services in common currency and
illustrates the cascading costs of damages as Nr changes
form and moves between different parts of the ecosystem.
In addition, human health implications can also be
included as the cost of health care treatment, lost work
days and other aspects of morbidity and mortality (e.g.,
economic value of lives lost). A third  metric is to look at
morbidity and/or mortality separately and not monetize
them with a cost value. Of course a concern, particularly
with respect to the economic metric, is that there are a
number of ecosystem services that arguably cannot be
easily monetized, for example the loss of biodiversity and
those ecosystem functions that are affected by climate
change or other stressors. Ecosystem services considered
to be regulating and supporting are particularly difficult
to fit into an economic metric. It is thus essential that
a variety of complementary metrics be used to assess
the impact of anthropogenic Nr on the environment and
human well being.
  There is value in each of the ways that N metrics
are expressed. Traditional categories provide a readily
adaptable framework for regulation, while ecosystem
service and function-based categories provide a richer
context for stating the complex connections among
Nr inputs and transformations and their impacts on
ecosystem health and human well-being. Dollar-based
metrics provide a means of identifying those effects that
have the greatest impacts and costs to society.
  Finding 14: The Committee finds that reliance on only
one approach for categorizing the measurement of Nr is
unlikely to result in the desired outcome of translating
N-induced degradation into the level of understanding
needed to develop support for implementing effective Nr
management strategies.
  Recommendation 14:  It is, therefore, recommended
that the EPA consider the impact of different metrics
and examine the full range of traditional and ecosystem
response categories, including economic and ecosystem
services, as a basis for expressing Nr impacts in the
environment, and for building better understanding and
support for integrated management efforts.

4.3. Water Quality Regulation and
Management
Aquatic thresholds
  In aquatic ecosystems, thresholds at which excess Nr
becomes a problem can be expressed as a management
goal such as a total maximum daily load (TMDL) or as a
critical load (CL). Under the authority of the CWA, EPA
has developed guidance for establishing numeric nutrient
criteria on an eco-regional basis for lakes and reservoirs,
streams and rivers, estuaries and coastal waters, and
wetlands. EPA has proposed specific numbers for lakes
and reservoirs and rivers and streams and protocols for
developing criteria for estuaries and wetlands. Each state is
advised to go through an assessment to determine the best
methodology for implementing numeric criteria (U.S EPA,
2000c, 2000c, 200 Ib, 2007e).  These criteria will identify
impaired waterbodies for which TMDLs may be required.
  The second type of threshold available for aquatic
ecosystems is the critical load (CL).  Unlike the TMDL,
the CL (in the U.S.) has no regulatory framework but
rather sets the threshold of Nr loading at which negative

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Box 2: Economic Impact and Metrics for Chesapeake Bay and Its Watershed

   Recently, the N cycle and the implications of the reactive nitrogen cascade were translated into economic terms
for the case of Chesapeake Bay (Moomaw and Birch, 2005). This approach has recently been updated with more
recent data, and the economic and health impacts of different forms of Nr in multiple ecosystems and media have
been estimated using better modeling methods (Birch et al., 2011). As an illustration, each of these metrics is shown
as a percentage of Nr fluxes in the Chesapeake Bay water and air shed in Figure 15. Abatement costs are summarized
in Table 12. Atmospheric emissions account for 37%
of Nr entering the watershed, but they account for
75% of the dollar damages and 76% of the mortality
(U.S. EPA, 2005c). Mitigation costs per tonne of
atmospherically released Nr are the lowest among the
three sources. Additions of Nr to terrestrial ecosystems
add 60% to the system, but contribute only 24% of the
damage costs and 24% of the mortality, and have the
highest mitigation costs. Freshwater releases, the second
most expensive to mitigate, account for the smallest
portion of Nr contributions to the system by any of the
metrics considered: only 4% of the Nr, 2% of the cost
damages, and none of the mortality losses (Birch et al.,
2011). Costs of Nr damage and health metrics provide
additional economic measures of the cost effectiveness
of actions to reduce a metric ton of Nr.
                                        100%
                                         90%
                                         80%
                                         70%
                                         60%
                                         50%
                                         40%
                                         30%
                                         20%
                                         10%
                                          0%
                                                                                              Atmospheric
                                                                                              Terrestrial
                                                                                              Freshwater
                                        Figure 15: Relative importance of all reactive
                                        nitrogen sources released into atmospheric,
                                        terrestrial, and freshwater media within the
                                        Chesapeake Bay Watershed utilizing four
                                        different metrics
                                        Source: Birch et al., 2011 (Figure 3, p. 173). Reprinted
                                        with permission; copyright 2011, American Chemical
                                        Society.
   The metrics of damage cost and mortality (morbidity
shows a similar pattern to mortality, but is only one-tenth
the damage cost) indicate that controlling emissions
of NOX from combustion and industrial processes
produces greater gains in protecting human health and
the environment than does reducing other Nr releases,
though the two sources are comparable in terms of
reactive forms of Nr released to the watershed. This
difference occurs because emissions to the  air  cascade
through more parts of the watershed ecosystem than
releases directly to the Bay. If human health effects are
monetized, then the economic gains are even greater
from reducing atmospheric emissions (see Figure 16).
   Figure 16 is a scatter plot  of all quantifiable damage
costs (including health impacts) relative to  metric tons
of Nr showing the significant difference in  emphasis
of the two metrics. Note that direct additions to the
environment from agriculture are about 370,000 tonnes
Nr/year, and cause $1.7 billion worth of damage.
Emissions of NOX from mobile sources represent only
180,000 metric tons Nr/year but cause nearly $4.4
billion in damages each year, of which $108 million
is attributable to nitrate loading of the Chesapeake
Bay, $3.9 billion to human morbidity and mortality,
and the remainder to other forms of damage, such as crop and commercial forest damage. Hence the releases of Nr
into the airshed from mobile  sources, which are only half the amount of agricultural releases to the watershed, cause
more than 2.5 times the economic damage of environmental additions from agriculture. This integrated inclusion of
atmospheric, terrestrial, and aquatic additions of Nr is not reflected in today's regulations
   Marginal abatement costs per metric ton Nr by source into each of the three media are provided in Table 12, and
demonstrate that the least costly abatement cost per metric ton of Nr also comes from atmospheric emission controls.
While most legislation constrains how cost for remediation can be considered,  it is useful to know where the lowest
cost options lie in setting priorities.


&
1

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     These multiple metrics provide several ways of looking at the nitrogen cascade and its impact on human health
   and the environment. However, there are many impacts that remain unaccounted for in any of these metrics. Some
   impacts might be quantified, but the necessary data have yet to be collected. Economic losses due to damage to
   commercial fisheries in the Bay are an example that is likely to be significant but has not yet been quantified.
   Similarly, economic losses due to climate change and ozone depletion from N2O emissions have not been fully
   evaluated. Impacts such as loss of biodiversity cannot be readily quantified at all, so it is desirable to consider a set of
   qualitative and non-quantified metrics in addition to the quantitative ones.
     Other parts of the country such as the Mississippi valley or the Central Valley of California are expected to show
   very different patterns of cost damages, with terrestrial and freshwater emissions causing proportionally higher
   damage costs, and emissions to the atmosphere causing a lower percentage of damages. But those very differences
   would assist EPA and the generators of those emissions in setting priorities for mitigation.
     It is important to recognize that Nr is not the only stressor that can affect both human and environmental health.
   Researchers are challenged to comprehensively understand cause-and-effect relationships in a complex environment
   and to balance management actions and costs to ensure that risk-minimizing management strategies are effectively
   implemented.
     As these multiple metrics indicate, decisions about which fluxes of Nr to mitigate depend upon which metric is
   utilized. The cascading economic costs of damage highlight the importance of regulating air emissions because of
   their impacts on human heath as well as their large contribution to the degradation of Chesapeake Bay water quality.
   Hence, if one is interested in reducing water impacts of Nr, the total reduction of damage may rely nearly as much
   on stricter enforcement of the Clean Air Act as the Clean Water Act. This challenges our traditional approach to
   regulation, but that is a consequence of comprehensively examining Nr guided by the nitrogen cascade.
                       Table 12: Marginal abatement cost per tonne ofNrby source
            *ion in the N case'
             where emitted
     Air
    lource/poiiuian
                                           Electric utilities/NOx14
                                              lndustrial/NOx 15
                                          Mobile sources/NOy 16
                                            Non-agricultural/NHs
aiemeni cost per tonne o
                                          $4,800
                                         $22,000
                                         $14,000
                                       No estimate
                                            Agriculture/nitrate 17
                                         $10,000
     Land
                                         Urban and mixed open land
                                               uses/nitrate 17
                                         $96,000
     Fresh water
Point sources/nitrates 17
          $18,000
impacts have been documented. Based extensively on
European work, CLs for aquatic ecosystems are Nr inputs
on the order of 2-15 kg N/ha/yr (Bobbink et al., 2010).
There are numerous locations within the U.S. where
deposition to surface waters falls within this range.

Water quality standards
   Section 303 of the CWA requires states to adopt
water quality standards and criteria that meet the state-
              identified designated uses (e.g., uses related to "fishable"
              and "swimmable") for each waterbody. Specifically, "a
              water quality standard defines the water quality goals of
              a water body, or portion thereof, by designating the use
              or uses to be made of the water and by setting criteria
              necessary to protect the uses" (40 CFR §  131.2). Further,
              "such standards serve the dual purposes of establishing
              the water quality goals for a specific water body and serve
14 See U.S. EPA, 2005c
15 See U.S. EPA, 1998
16 See Krupnick et al., 1998
17 See Chesapeake Bay Program, 2003a,b

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as the regulatory basis for the establishment of water
quality-based treatment controls and strategies beyond the
technology-based levels of treatment required by sections
301(b) and 306 of the Act" (40 CFR §  131.2).
   The EPA sets minimum requirements for approvable
standards and criteria including: use designations;
water quality criteria sufficient to protect the designated
uses; and an antidigradation policy (40 CFR § 131.6).
Traditionally, Nr and other land, air, and water pollutants
are measured in terms of quantity (mass) released per unit
time (e.g., kg/day) or as a concentration (e.g., milligrams
per liter, ml/L). Therefore, regulations  often specify mass
loading limits or maximum concentrations in permits.
   In the mid-to-late 1990s, EPA began to emphasize the
development of numeric nutrient criteria for both P and
N through the state standards-setting process because,
according to the 1996 Water Quality Report to Congress
(U.S.EPA, 1997), 40% of the rivers, 51% of the lakes and
ponds, and 57% of the estuaries assessed for the report
were exhibiting a nutrient-related impairment. Few states
had adopted numeric nutrient criteria for all affected
waterbodies, especially for N, often relying on narrative
criteria or secondary effects such as chlorophyll-a
concentration, dissolved O2, or water clarity. EPA's
strategy, driven by President Clinton's Clean Water
Action Plan (U.S. EPA and USD A, 1998) mandated
numeric nutrient criteria to begin to address the problem
(U.S. EPA, 1999). To move the objectives of the Clean
Water Action Plan forward, EPA published national
nutrient criteria guidance for lakes and reservoirs (U.S.
EPA, 2000d), rivers and streams (U.S.  EPA, 2000b),
estuaries and coastal waters (U.S. EPA, 200Ib), and
wetlands (U.S.  EPA, 2007c), based upon ecoregional
guidance for lakes and reservoirs and rivers and streams.
To date, relatively few states have adopted new numeric
criteria into their water quality standards. While some
successes are evident in promulgating  P criteria for
freshwater systems, which has a richer history of numeric
criteria incorporation into state water quality standards,
development of numeric nitrogen criteria has been elusive
for a variety of reasons.
   Nr management in multiple media and across
jurisdictions can be complicated because the CWA has
little authority over atmospheric sources, and individual
states explicitly lack authority to control upstream
sources. For example, extensive monitoring and analysis
of the sources of reactive nitrogen in the Raccoon
River of western Iowa have shown that point sources
from municipal treatment plants and residential septic
tanks account for less than 8% of the total nitrogen
load to the system, with agricultural runoff being the
overwhelming source (Jha et al., 2010). This disparity is
similar statewide (Libra et al., 2004). As a result, nutrient
management strategies that are focused on the control
of point sources can often result in inefficient allocation
of resources if non-point sources are not also addressed.
In addition it is often the case for estuaries such as the
Gulf of Mexico or Chesapeake Bay, that management
goals that meet water quality standards cannot be attained
without interstate compacts or a strong federal role. This
may be resisted by upstream states that may have to bear
the cost but do not necessarily reap the benefits of the
water quality improvement. Such a dilemma underscores
the need for an integrated approach to Nr management.
The Committee notes that a State-EPA Nutrient
Innovations Task Group has considered some options for
improving control of nutrient pollution sources (State-
EPA Nutrient Innovations Task Group, 2009).
   Populated (urban/suburban/developed) land areas
provide significant loads of Nrto the environment, both
by generation (e.g., deposition of NOX emissions) and
by transfer (e.g., domestic sewage from food imported
into the watershed). Categorical sources include sewage
treatment plants (STPs), industries, subsurface (septic)
systems, atmospheric deposition, domestic animal and
wildlife waste, and fertilizers used on lawns, gardens
and landscapes. Infrastructure (e.g., storm sewers) and
landscape conditions (e.g., increased impervious cover)
more efficiently move Nr associated with surface runoff
to receiving waters and may also inject or infiltrate Nr into
ground water. Landscape changes, primarily increases in
impervious cover, soil disturbance and compaction, and
wetland/hydric soil losses, have also reduced the capacity
for natural systems to treat Nr inputs by recycling or
denitrification.  Other disruptions in chemical condition
(e.g., acidification), biology (e.g., vegetative cover), and
physical character (e.g., temperature increase) alter the
nitrogen cascade, which may have both negative and
positive consequences for Nr amelioration on the populated
landscape and in air and water. Populated lands are
estimated to export as much as 10 times the total nitrogen
that was exported under pre-development conditions.
   Finding 15: Intervention to  control Nr under most
water management programs generally occurs in three
ways:
  Prevention or source controls.
  Physical, chemical, or biological "dead ending" or
  storage within landscape  compartments where it is
  rendered less harmful (e.g., long-term storage in soils
  or vegetation; denitrification, primarily in wetlands;
  reuse).
• Treatment using engineered systems such as wastewater
  treatment plants or BMPs for stormwater and nonpoint
  source runoff.
   While most management programs focus on the
third (treatment) approach, there are opportunities for
combining the three that can be more effective and cost
less. Furthermore, it is important to recognize that in
some cases total reduction of water impacts of Nr may
rely nearly as much on stricter enforcement of the Clean
Air Act as the Clean Water Act.

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   Recommendation 15: To better address Nr runoff
and discharges from the peopled landscape the Committee
recommends that EPA:
15a.
• Evaluate the suite of regulatory and non-regulatory
 tools used to manage Nr in populated areas from
 nonpoint sources, stormwater and domestic sewage,
 and industrial wastewater treatment facilities,
 including goal-setting through water quality standards
 and criteria.
• Determine the most effective regulatory and voluntary
 mechanisms to apply to each source type (recognizing
 that in some cases total reduction of the impacts ofNr
 may rely nearly as much on stricter enforcement of
 the Clean Air Act as the Clean Water Act) with special
 attention to the need to regulate nonpoint source and
 related land use practices.
15b.
• Review current regulatory practices for point sources,
 including both wastewater treatment plants and
 stormwater, to determine adequacy and capacity
 towards meeting national Nr management goals.
• Consider technology limitations, multiple pollutant
 benefits, and funding mechanisms as well as potential
 impacts on climate change from energy use and
 greenhouse gas emissions,  including nitrous oxide.
15c.
• SetNr management goals on a regional/local basis,
 as appropriate, to ensure most effective use of limited
 management dollars.
• Fully consider "green " management practices such as
 low- impact development and conservation measures
 that preserve or re-establish Nr-removing features to the
 landscape as part of an integrated management strategy,
 along with traditional engineered best management
 practices.
15d.
• Research best management practices that are effective in
 controlling Nr, especially for nonpoint and stormwater
 sources, including land and landscape feature preservation
 and set Nr management targets that realistically reflect
 these management and preservation capacities.
• Construct a decision framework to assess and determine
 implementation actions consistent with management goals.
15e.
• In cooperation with the Departments of Agriculture and
 Army, the Fish and Wildlife Service and the Federal
 Emergency Management Agency, the EPA should
 develop programs to encourage wetland restoration
 and creation with  strategic placement of these wetlands
 where reactive nitrogen is highest in ditches, streams,
 and rivers. The Agency should also address the means
 of financing, governance, monitoring, and verification.
 Such programs might be modeled on the Conservation
 Reserve Program  or extant water quality and
 environmental trading programs, but need not be limited
 to current practices (as discussed in section 5.3.4).

4.4. Water Quality Monitoring
and Assessment
   Under Section 106 of the CWA, the EPA provides
funds to assist state and interstate agencies and tribes
to conduct monitoring of the nation's waters to ensure
adopted water quality criteria and designated uses are
met. Further, primarily under Section 305(b) of the CWA,
those entities are required to report, on a biennial basis, on
the health and status of their jurisdictional waters. These
assessments are presented by the states to the EPA to
categorize attainment of designated uses. EPA published
these reports up until 1998 (U.S. EPA, 2000a), after
which it transitioned into a Water Quality Report in 2000
(U.S. EPA, 2002) and a National Assessment Database
in 2002 (U.S. EPA, 2010c). States also prepare a list of
"impaired" waters under Section 303(d) of the CWA and
EPA develops a synthesis of the CWA Section 305(b) and
303(d) reporting under a Consolidated Assessment and
Listing Methodology (CALM) approach.
   As discussed above, the EPA compiles the approved
state 303(d) lists into a national listing (U.S. EPA,
2010e). The list provides information by state as well
as by impairment cause, and identifies the TMDLs
completed to date. The most current data available
on the EPA Web site includes reporting from most
entities through 2008. The report identifies 6,816
impairments related to "nutrients" (almost 9% of all
identified impairments),  although other impairments
may ultimately have a nutrient enrichment cause. For
example, organic enrichment/oxygen depletion (6,410),
turbidity (3,046), noxious aquatic plants (981), algal
growth (539), and ammonia (general toxicity 356),  can
all have a common cause such as N or P enrichment. It
should also be clear that impairments may have multiple
causes so, for example, waters identified as impaired by
C>2 depletion may  also be impaired by nutrients.
   There are other initiatives promoted by EPA to
monitor and assess the nation's waters, generally
implemented in collaboration with,  or by, the state and
interstate agencies and tribes having jurisdiction over the
waters. These include the Wadeable Stream Assessment
(U.S. EPA, 2006c), the National Coastal Assessment
and its National Coastal  Condition Reports (U.S. EPA,
200 la, 2004a, 2006b), the Survey of the Nation's Lakes
and Survey of the  Nation's Rivers and Streams, and
more recently, probabilistic monitoring efforts in lakes,
streams, and estuaries (U.S. EPA, 2010d). Many of
these are aimed at including a biological assessment
component that is often lacking in water pollutant and
chemistry efforts described above.
   The USGS collects data on surface and underground
waters and disseminates these data to the public, state and
local governments, public and private utilities, and other

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federal agencies involved with managing water resources.
The Committee encourages EPA to work closely with
USGS on monitoring and assessment activities.
   The National Oceanic and Atmospheric Administration
has periodically produced estuarine assessments under
the National Estuarine Eutrophication Assessment
(NEEA) program. The most recent report was released
in 2007 (Bricker et al, 2007). The report has a focus on
nutrient enrichment and its manifestations in the estuarine
environment and relies on participation and interviews of
local experts to provide data for the assessment. Among
the key findings were:
   Eutrophication is a widespread problem, with the
   majority of assessed estuaries showing signs of
   eutrophication - 65% of the assessed systems,
   representing 78% of assessed estuarine area, had
   moderate to high overall eutrophic conditions.
   The most common symptoms of eutrophication were
   high spatial coverage and frequency of elevated
   chlorophyll-a (phytoplankton) - 50% of the assessed
   estuaries, representing 72% of assessed area, had
   excessive chlorophyll-a ratings.

4.5.  Clean Air Act  and Air Quality
Regulation and Management
   The modern history of American air pollution control
legislation begins with the 1963 Clean Air Act (CAA)
which, along with its amendments, requires the EPA
to establish and revise National Ambient Air Quality
Standards (NAAQS) and to prepare state of the science
reviews such as the Criteria Documents and more recently
the Integrated Science Assessments (ISA)  (U.S. EPA,
2005a, 2006a, 2007a). There are six criteria pollutants:
carbon monoxide, lead, NC>2, ozone, 862, and PM.
These have been determined to endanger public health
or welfare. The CAA as currently written requires a
review of the scientific criteria for these standards at five-
year intervals. Although NC>2 is the only Nr compound
specified as a criteria pollutant, NHX and NOy play a
major role in formation of the secondary pollutants ozone
and paniculate matter.
  The CAA has been amended several times since its
inception. In 1970, the CAA was amended "to provide
for a more effective program to improve the quality of
the nation's air." The CAA was amended again in 1977,
primarily to mandate reductions of emissions from
automobiles. Despite evidence that NOX is the central
pollutant in photochemical smog formation (Chameides
and Walker, 1973; Crutzen, 1973, 1974; Fishmanand
Crutzen, 1978; Fishman, et al., 1979), federal regulations
did not require automobiles to control NOX emissions to
below 1 g/mi (0.14 gNperkm) until 1981. Few locales
violate the standards for NC>2,18 but the secondary effects
of several of these gases also pose health and welfare
concerns. If a city had an annual average NO2  level
   Table 13: Federal primary ambient air quality standards that involve Nr, effective February 2010.
Pollutant
Ozone (Os)
1-hr average
8-hr average

Nitrogen Dioxide (NO2)
1-hr average
Annual average

Particulate Matter, coarse (PM-io)
Diameter < 10 |jm, 24-hr average
Annual average
Particulate Matter, fine (PM2.s)
Diameter < 2.5 urn, 24-hr average
Annual average
Federal Primary Standard (NAAQS)
0.12 ppmv
0.08 ppmv

100 ppb
0.053 ppmv (100 ug/m3)

1 50 ug/m3
50 ug/nr
35 ug/m3
1 5 ug/m3
Note: Secondary standards are currently identical to the primary standards. Source: www.epa.gov/air/criteria.html
18 In 2010, EPA promulgated a new 1-hour standard of 100 ppb for NC>2 [Primary National Ambient Air Quality Standards for Nitrogen Dioxide; Final
  Rule, Federal Register 75 (26): 6474-6537]. Monitoring for compliance with this new standard is required, but it will not be known for several years
  which if any locales violate this standard.

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anywhere near the NAAQS for NO2, it would risk severe
photochemical smog - the summertime efficiency for
ozone production ranges from 4 to 10 ppb 63 per ppb NOX.
   As previously discussed, the focus on compliance
monitoring for NO2 ignores the other, equally important
members of the NOy family such as HNOs that deposits
quickly onto the earth's surface. It is clear that a causal
relationship exists between current levels of N and S
deposition and numerous biologically adverse effects
on ecosystems across the U.S. (U.S. EPA, 2008d).
Conversion of the existing network of NOX monitors to
NOy monitors with a detection limit of 0.1 ppb would
still demonstrate compliance with the NO2 standard but
greatly increase the utility of the measurements for model
evaluation as well as for understanding nitrate deposition
and formation of photochemical smog, and haze.
   Air pollution, especially ozone and PM, continued to
be a problem in many American cities and the CAA was
again amended in 1990. The Nr-relevant aspects were
aimed at controlling urban smog and acid deposition.
States were required to develop emissions inventories for
reactive organic  compounds, carbon monoxide, and NOX,
but not NH3 or N2O. Over the U.S., sulfate and nitrate are
responsible for about two-thirds and one-third, respectively,
of the direct deposition of acids. The CAA Amendment of
1990 required emissions decreases of 10 million tons of SO2
and 2 million tons of NOX relative to 1980 levels. Ammonia
and ammonium, although they contribute to acidity after
entering terrestrial ecosystems (Galloway et al., 2003; NRC,
2003) and are expected to play an increasing role (Pinder et
al., 2008), were not regulated by this legislation.
   The 1997 revision of the CAA and related regulations
changed the standards for ozone and PM (see Table 13). A
sizable fraction of the mass of PM less than 2.5 microns,
PM2 5, is condensed Nr. As stated above, these particles
have adverse health consequences. PM is also controlled
by the Regional  Haze Regulations (40 CFR 51). These
regulations require that by the year 2064, states must
restore Class I areas defined in the regulations to their
natural levels of atmospheric clarity.
   Ozone and PM, the two most recalcitrant of the criteria
pollutants, cover large spatial scales. All of the ozone and
much of PM are secondary pollutants in that they are not
released at the tailpipe but form in the atmosphere.  Ample
evidence shows  that much or most of the PM in American
cities is secondary (e.g., Donahue et al., 2009). Violations
are declared on urban scales, responsibility for their control
was assigned to  states, but the physics and chemistry of
smog and haze are regional. In the eastern U.S., ozone
episodes often cover several  states and involve pollutants
emitted in upwind states that do not themselves experience
violations (Husar et al., 1977; Logan 1989; Moy et al.,
1994; Ryan et al. 1998). The 1990 amendments to the Clean
Air Act authorized, in part as a response to this scaling
problem, the Ozone Transport Assessment Group (OTAG)
and the Ozone Transport Commission (OTC). These have
jurisdiction extending from Washington, D.C. to Maine.
Progress has been made on regional control of emissions;
the NOX State Implementation Plan (SIP) call, implemented
in 2003 and 2004, has led to measurable improvements in
ambient ozone and nitrate levels (Gego et al., 2007; Sickles
and Shadwick, 2007a). Experiences with ozone and PM
provide a useful demonstration of why it is necessary to
develop an integrated approach to management of Nr.

Atmospheric thresholds for Nr
   As shown in Table 13 the metric used for safe, upper
limits in the atmospheric environment is concentration
(in mass per unit volume of air or volume mixing ratios)
averaged for a given time period, usually 1 hour, 8 hours,
24 hours, or annually. The thresholds for excess Nr in the
atmosphere remain an area of active research. The only
Nr compound for which there is currently a NAAQS is
NO2, which may not exceed 0.053 ppm  (100 ug/m3) for
the annual arithmetic mean and 100 ppb for the one-hour
average. This standard, based on the direct health effects,
is certainly inadequate because NO2 concentrations well
below 0.053 ppm lead to concentrations of secondary
pollutants well above acceptable levels (i.e., PM2 5 and 03).
The NO2 concentration required to achieve the current 75
ppb ozone standard has not been rigorously established,
but it must be well below 0.053 ppm, because information
provided by EPA indicates that areas currently in violation
of the ozone standard typically have NO2 concentrations
below 0.020 ppm (U.S.EPA, 2010a). The NO2 concentration
required to achieve the current 15 ug/m3 PM2 5 standard
is probably also below the 100 ug/m3  standard for NO2
because of the role of NO2 in secondary paniculate
formation. States in the eastern U.S. are considering
substantial additional NOX emissions reductions in order to
comply with the new 8-hour 75 ppb ozone standard. One
scenario being tested (G. Aburn, Maryland Department
of Environment, personal communication) involves the
following reductions: (1) reducing NOX emissions for point
sources by 65%, (2) reducing NOX emissions for on-road
sources by 75 percent, (3) reducing NOX emissions for
nonroad sources by 35%, and (4) reducing VOC emissions
by 30% for all source groups.
   As further discussed in Section 6.2, it is the opinion of
the Committee that a decrease in NOX emissions of 2 Tg N/
yr relative to the 2002 baseline level can be achieved in the
near term. Emissions decreases implemented since 2002
have already substantially improved ozone concentrations
(Gego et al., 2007). The absolute amount of decrease and
the positive impact it would have on human health is region
dependent, but further decreases will result in further
beneficial decreases in PM2 5 and 03 concentrations.
   The threshold for total Nr in the atmosphere is yet to be
fixed, but depends on its rate of deposition to the surface
and the sensitivity of the receptor(s). The immediate
need for determining thresholds for atmospheric Nr is
monitoring of NOy and NHX.

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4.6. Thresholds for Excess Nr Effects on
Terrestrial Ecosystems
   In parallel with the original concept of critical loads
developed by Nilsson and Grennfelt in 1988 and now
widely used for air quality management in Europe
(Appendix D), thresholds in general and critical loads
specifically for Nr effects on terrestrial ecosystems in the
U.S. should be understood to be "quantitative estimates
of exposure to air concentrations of Nr compounds below
which harmful effects on specified sensitive elements
within ecosystem of concern do not occur according
to present knowledge" (Nilsson and Grennfelt,  1988;
Heittelinghetal, 2001).
   In developing these quantitative estimates of thresholds
and/or critical loads for terrestrial ecosystems in the U.S.
(e.g., Fenn et al., 2003), it is imperative to understand
the extraordinarily wide diversity and Nr-sensitivity
of various components of terrestrial ecosystems in
different parts of the U.S., as well as the huge differences
in purposes and intensity of management and public
perceptions of the value of these ecosystem components
to various sectors of American society. Thus, the critical
loads appropriate for maintaining species diversity in a
natural grassland in northern Minnesota or a wilderness
area in the Mediterranean climate of southern California
are likely to be very different from those associated
with direct effects on similar systems in other regions
of the U. S. - or even for beneficial and/or adverse
effects on other components of the same terrestrial
ecosystem. For example, the threshold or critical load for
adverse effects of excess Nr on understory vegetation,
beneficial mycorrhizae,  or lichen communities in a
forest ecosystem is likely to be very different from the
threshold for adverse effects on the dominant forest trees
in that same ecosystem. Thus, public perceptions of
"specified sensitive elements within the ecosystem" may
be important in determining what specific thresholds or
critical loads should be considered in order to minimize or
avoid specific adverse effects of concern.
   At present, the sum total of directly measured wet
plus dry-deposited chemically oxidized (NOy) and
chemically reduced (NHX) inorganic Nr loads in various
states within the contiguous U. S. are on the order of 3
to 15 kg N/ha/year (NADP, 2010; CASTNET, 2010). As
shown in Appendix A, a three-year run of the Community
Multiscale Air Quality (CMAQ) model also provided
estimates of the average annual total Nr loads (including
organic forms as well as inorganic NOy and NHX forms of
Nr) in the contiguous U.S. These model estimates varied
from minimal deposition values of about 3 kg N/ha/year to
maximum estimated values of about 17 kg N/ha/year. This
range agrees well with the range of the measurements.
   These directly measured and modeled estimates of total
(wet plus dry) deposition of organic and inorganic forms
of Nr indicate that there are several areas, especially in
the eastern U.S., and a few areas of the western U.S.,
where current total Nr loads are already very close to, or
will very likely soon exceed, the recommended threshold
and critical load estimates provided by Bobbink et al.
(2010) in their review of scientific evidence regarding
the impacts of atmospheric nitrogen deposition on plant
diversity in terrestrial ecosystems.

4.7. Comments on Nr Critical Loads
   In recent years, the Acid Rain Action Plan developed
by New England governors and eastern Canadian
Premiers has led to evaluations of critical loads to surface
waters and forests in that region. Those studies identified
many waters and forest lands that met or exceeded
critical load capacity for combined sulfur and nitrogen
deposition both in the New England States and in the
eastern Canadian provinces. The plan set target decreases
of 20 to 30% for nitrogen oxide emissions by 2007 and a
50% decrease in sulfur dioxide emissions by 2010. These
targets are intended to decrease long-range transport of
air pollutants, acid deposition, and nutrient enrichment of
marine waters  in this region.
   In May 2006, a Multi-Agency Critical Loads Workshop
was held, which led to the formation of a Critical
Loads Ad-Hoc Committee (CLAD) within the National
Atmospheric Deposition Program (NADP).  A goal of
the program is  to "provide consistency in development
and use of critical loads in the U.S." One outcome is a
project undertaken by the Northeast States for Coordinated
Air Use Management (NESCAUM) to: "estimate critical
loads of sulfur  and nitrogen in atmospheric deposition
for areas where sufficient knowledge, data, and methods
exist" and "to demonstrate the use of critical loads as a tool
for assessing environmental policies and programs and
managing natural resources."
   A February 2007 Workshop sponsored by EPA on
"The Assessment of Health Science for the Review of the
National Ambient Air Quality Standards (NAAQS) for
Nitrogen (NOX) and Sulfur Oxides (SOX)" expansively
reviewed both ecosystem and human health effects toward
revision of the NAAQS. Policy discussions at this workshop
raised the questions of whether critical loads assessments
were an effective means of improving ecosystem
management, and whether the science was understood well
enough to use critical loads as a management tool. The
conclusion was that, although there was a substantial body
of accumulated scientific evidence, there was only limited
use of critical loads approaches for management of air
quality in the U.S. The Multi-Agency Workshop on Critical
Loads (mentioned above) was cited at EPA's 2007 workshop
as an agenda-setting effort to resolve some of the science
and policy issues that could help advance critical loads
approaches in the U.S. The Integrated Nitrogen Committee
believes that the primary reason critical loads are not now
used in the U.S. is that policy makers in this country have
so far not been willing to adopt unfamiliar air and water
quality management approaches or approaches that have not

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been evaluated directly in this country. Thus, the Committee
recommends that EPA consider implementation of the
critical loads concept for management of deleterious Nr
effects in various parts of the U.S.
   Finding 16: The Committee finds that there have been
persistent increases in the amounts of Nr that have been
emitted into and retained within various ecosystems,
affecting their functioning. Unless this trend is reversed,
it will become increasingly difficult for many of these
ecosystems to provide the services upon which human
well-being is dependent. The Committee believes that
there is a need to regulate certain forms of Nr to address
specific problems related to excess Nr, and we believe that
the best approach for an overall management strategy is
the concept of defining acceptable total Nr critical loads
for a given environmental system.
   Recommendation 16: The Committee recommends
that the Agency work toward adopting the critical loads
approach concept in determining thresholds for effects
of excess Nr on terrestrial and aquatic ecosystems.
In carrying out this recommendation the Committee
recognizes that it will in many cases be necessary for
the Agency to enter into new types of research, policy,
and regulatory agreements with other federal, state, and
tribal units based on cooperative, adaptive, and systemic
approaches that derive from a common understanding of
the nitrogen cascade.

4.8. Tradeoffs of Nr  Impacts in Risk
Reduction Strategies
   Because nitrogen is such an abundant and widespread
element, and Nr is such a critical component of the earth's
biosphere, associated impacts are many and pervasive.
In many cases, strategies to manage the impacts of Nr
involve tradeoffs, i.e., mitigating one type of impact may
exacerbate others. Given the interactions among oxidized
and reduced N species, it is important to recognize the
potential for unintended consequences to occur as a result
of strategies that are aimed at limiting one form of Nr in air
or water but lead to the increased production of other forms
of Nr, or the formation and release of other contaminants
of concern. For example, stringent control of point sources
of Nr can be energy-intensive, requiring significant energy
investments for chemicals, electricity, and other support,
and this may in turn lead to the production of more reactive
nitrogen and increased CO2 emissions. Furthermore,
there may be environmental  impacts of these treatment
processes, particularly in the production of solid wastes
that can be significant environmental hazards. This is
the main reason that a life cycle approach is necessary
in evaluating any remediation or treatment scheme. In
addition, as discussed in Section 3.1.2, numerous lakes,
reservoirs, rivers, and fjords  worldwide exhibit N and P co-
limitation, either simultaneously or in seasonally-shifting
patterns. Therefore, strategies are needed to reduce both
P and N inputs. Not all control practices will be effective
for dual nutrient reduction and this must be taken into
consideration. Four categories of tradeoffs examined below
are: ammonia release from concentrated feed lot operations
(CAFOs), concerns about human nutrition, nitrification and
denitrification, and nitrogen-carbon related impacts.

Ammonia release from CAFOs
   As a result of effluent guidelines for NH3 in aquatic
systems,  state and federal regulations and programs
under the CWA were developed to address water
quality protection from CAFOs. The resulting manure
management systems utilized NH3 volatilization as a
means to remove N and decrease the N in the manure
when land applied. Only recently has the resulting
increase in NH3 emission into the air been viewed as a
potential problem with respect to air quality concerns and
N deposition.
   Finding 17: Current EPA policy (40 CFR Part 51,
Clean Air Fine Particle Implementation Rule) discourages
states from controlling ammonia emissions as part of
their plan for reducing PM2 5 concentrations. In this
rulemaking, EPA states that "ammonia reductions
may be effective and appropriate for reducing PM2 5
concentrations in selected locations, but in other locations
such reductions may lead to minimal reductions in PM2 5
concentrations and increased atmospheric acidity."
Ammonia is a substantial component of PM2 5 in most
polluted areas of the United States at most times. While
it is true that reducing NH3 emissions might increase the
acidity of aerosols and precipitation, the net effect of NH3
on aquatic and terrestrial ecosystems is to increase acidity.
After being deposited onto  the earth's surface, NH4+ is
under most circumstances quickly nitrified, increasing the
acidity of soils and waters.  The Committee is unaware of
any evidence that NH3 reduces the toxicity of atmospheric
aerosols or that high concentrations of NH3 occur
naturally over any substantive area of the United States.
It has not yet been established which components of PM
have substantive impacts on human health, but the total
concentration of PM2 5 correlates with morbidity and
mortality, and NH3 contributes to PM2 5. The visibility
degradation and other adverse effects associated with
PM2 5 are related to aerosol surface area or mass where
NH4+ certainly plays a role.
   Recommendation 17: The Committee recommends that
the EPA presumption that NHj is not a PM2.5 precursor
should be reversed and states should be encouraged to
address NH$ as a harmful PM^ 5 precursor.

Swapping N between environmental systems
   Nitrous oxide is produced in "natural" and agricultural
soils, and all aquatic systems, almost exclusively as
a result of the microbial processes of nitrification and
denitrification. As NH4+ ion is the initial mineral N
product formed during organic matter mineralization
and most of the fertilizer used worldwide is NH4+ based
(e.g., urea, ammonium sulfate) (FAO, 2007), the suite

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of microbiological reactions that result in the release of
gaseous N products need to be considered.
  Nitrification is the oxidation of NH4+ ion to
NO3~ (Figure 17). Most commonly, nitrification is a
chemolithotropic process consisting of the conversion of
ammonium to nitrite, which is then converted to NCV by
a second group of bacteria. The ammonium oxidizing
bacteria (AOB) are obligate aerobes with some species
that are tolerant of low-oxygen environments. The most
common genera of autotrophic NH4+ oxidizers are
Nitrosospira and Nitrosomonas. AOB are found in most
aerobic environments where ammonium is available
through the mineralization of organic matter or where N
compounds are added.
  Biological denitrification is the dissimilatory reduction
of NO3~ and nitrite to produce NO, N2O, and N2 by a
taxonomically diverse group of bacteria. These bacteria
synthesize a series of reductases that enable them to
utilize  successively more reduced N oxides as electron
acceptors in the absence of oxygen. The general reductive
sequence is shown in Figure 17. In addition to the
free-living denitrifiers, symbolically -living Rhizobia in
root nodules of legumes are able to  denitrify nitrate and
produce nitrous oxide (Mosier and Parkin, 2007).
  The abundant denitrifiers are heterotrophs, which
require sources of electron-reducing equivalents contained
in available organic matter. Factors that most strongly
influence denitrification are oxygen,  nitrate concentration,
pH, temperature, and organic carbon. The reductive
enzymes are repressed by oxygen but not by NH4+.
Nitrous oxide reductase  appears to be more sensitive to
oxygen than either NOs" or nitrite  reductase. Therefore N2
production predominates in more anoxic sites and N2O
production may be greater in more aerobic conditions.
However, the ratio of N2 to N2O emitted may also be
affected by high NOs" concentrations and associated higher
levels of electrical conductivity and osmotic stress and soil
pH (low pH favors N2O production).
  Given these interactions among  oxidized and
reduced N species (discussed above), it is important to
recognize the potential for unintended consequences
to occur as a result of strategies that may be aimed at
limiting one form of Nr in air or water but lead to the
increased production of other forms of Nr. One such
instance is the potential offsetting of the benefits of
NO3~ remediation at the expense  of increasing input of
N2O to the atmosphere.  An example of such a situation
involves NOs" leached from agricultural fields, much of
which could be removed from drainage water in natural
or reconstructed wetlands. This process is ideal if the
denitrification process goes to completion, i.e., only
N2 is produced. If, however, the process is incomplete,
and NO and N2O gases are emitted, then the end result
may  create a compensating risk that could be greater
than that posed by the nitrate that is removed. This is
   Nlrrosomonas
   Nttrosospira
   NitmsocGccus
Main Controls
Substrate. Oz, H3O, T
                         -a
                           >^
                             r •
              PI*
>^     Ntirvbacte t
                 NjO
                      DcnitrifU-Htion
                           • NO
                                      NO,
             F.iculLitive Anaerobic Bacterij
             Main Controls
             Substrate, available C.Oj, HjO, T

Figure 17: Diagram of the nitrification and
denitrification processes

Source: Mosier and Parkin, 2007. Reprinted with
permission; copyright 2007, Taylor & Francis Group LLC
- Books.
because NO continues to be reactive in the atmosphere
and is eventually redeposited in aquatic or terrestrial
systems, and N2O is a GHG that has an atmospheric
life time of approximately 100 years and a radiative
forcing of approximately 300 times that of CO2 on a
hundred-year time frame (IPCC, 2001). N2O is also a
major source of NO in the stratosphere and depletes
stratospheric ozone (Crutzen, 1981). If more of the NOs"
denitrified is converted to N2O in wetlands than upstream
or downstream, the environmental cost may be high.
Hernandez and Mitsch (2007) found that permanently
flooded wetlands had lower N2O/N2 ratios of emissions
than did intermittently flooded wetlands. They also found
that the ratio was higher in the cold months even though
the flux rates are much lower then. A full risk assessment
needs to be made to determine how much of such
"pollutant swapping" is advisable.
   A similar potential exists for Nr mediation in sewage
treatment. The current practice is to convert ammonia/
ammonium that mineralizes from excreted organic matter
to nitrate through the nitrification process. As nitrate-
containing effluent from sewage treatment flows into
aquatic systems the nitrate may be denitrified, resulting
in N2O production if denitrification is not complete. The
protein consumption by some 301 million humans in the
U.S. results in the processing of ~ 2 Tg of N annually
(-18.4 g N/ person/day),  much of which flows through
sewage treatment facilities and ultimately leads to the
production of between 0.06 and 0.1 Tg of N2O-N /yr in
aquatic systems or soils to which sewage sludge is applied.

Tradeoffs among C and N-driven impacts
   Reactive N also contributes to many impacts on the
environment that are also impacted by other chemical
species, notably carbon. As depicted in Figure 18, there
are several points of tangency between the global C and
N cycles. These are: combustion, agricultural production,
industrial production, soil and sediment processes, and

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end-of-life disposition of products. The implication of these
interactions is that, in many instances, the perturbation
of one cycle cannot be fully assessed without including
effects on the other. For example, proposals to develop
bio-based products (biofuels, but also other products) as the
preferable alternative to fossil-based resources are not free
from impacts. Such trade-offs may involve a single impact
(e.g., global climate change to which both carbonaceous
gases and N2O contribute) but may also involve trade-offs
between impacts that are not easily compared. Figure 19
shows the latter case in the form of climate change impacts
(to which C is a principal contributor) versus eutrophication
impacts (to which nitrogen is a principal contributor) for
several different biofeedstock-product combinations which
are evaluated relative to the substituted commercial product
made from fossil C. A value of 100% on the y-axis would
mean that the bio-based alternative is no better than the
fossil-based counter-product, while the negative region
of the y-axis in Figure 19 represents net C sequestration.
It is difficult to make direct comparisons across disparate
impact categories, however Figure 19 suggests that, in
choosing among alternatives, policies that aim to minimize
both sets of impacts would be preferred.
                                                      Finding 18: The Committee notes that the effective
                                                   management of Nr in the environment must recognize the
                                                   existence of tradeoffs across impact categories involving
                                                   Nr transformations and the cycling of other elements.
                                                      Recommendation 18: The Committee recommends
                                                   that the integrated strategies for Nr management outlined
                                                   in this report be developed in cognizance of the tradeoffs
                                                   associated with reactive nitrogen in the environment
                                                   (consistent with the systems approach of overarching
                                                   recommendations 2 and 3 discussed in Section 6.2 of this
                                                   report). Specific actions should include:
                                                      Establishing a framework for the integrated
                                                      management of carbon and reactive nitrogen;
                                                      Implementing a research program that addresses the
                                                      impacts of tradeoffs associated with management
                                                      strategies for carbon, reactive nitrogen, phosphorus,
                                                      and other contaminants of concern;
                                                      Implementing a research and monitoring program
                                                      aimed at developing an understanding of the combined
                                                      impacts of different nitrogen management strategies
                                                      on the interchange of reactive nitrogen across
                                                      environmental media.
C flows     N flows

 Bioproduct, material,
 and energy flows
                                                                                        ATMOSPHERE
                                         FOSSIL FUELS AND
                                            CHEMICAL
                                           PRODUCTION
                                          Energy and chemicals
Figure 18: Combined carbon and nitrogen global cycles
Source: Miller et al., 2007 (Figure 1, p. 5178). Reprinted with permission; copyright 2007, American Chemical Society.

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4.9. Interactions of the N Cascade
and Climate
   Weather and climate vary substantially on many time
scales including the interannual. Long-term (decadal or
more) changes in climate as have been predicted by IPCC
(2007a,b) may have profound effects on the N cycle;
conversely, changes in the biogeochemical cycle of Nr can
induce climate forcing. While it is beyond the scope of

         Carbon Nitrogen Tradeoffs for Various Bloproducts
                               CSET
              0.5  *SL 1.0     1.5     2.0     2.5      3.0
                   Eutrophlcatlon : •. n i m..i -, KIO,-'MJ)
Figure 19: Comparisons between Global
Warming Potential (GWP) and eutrophication
impact categories for various bioproducts
Abbreviations: BD=Biodiesel; CET=Corn Ethanol;
CSET=Corn & Stover Ethanol; PLA=Polylactic Acid
(Corn); RL=Rapeseed Lubricant; SL=Soybean Lubricant;
STET=Stover ethanol; SWEL=Switchgrass Electricity;
SWET=Switchgrass Ethanol.
Source: Adapted from Miller et al.,  2007 (Figure 2,
p. 5180). Adapted with permission; copyright 2007,
American Chemical Society.
this report to fully address how cycles of C and N interact
(see Figure  18 for a general treatment of the intersection
points of C and N cycles), there are several ways in which
climate impacts the biogeochemical cycle of Nr and vice
versa (e.g., Yienger and Levy, 1995; Holland etal., 1997;
Hungate et al., 2003; Hungate et al., 2004; Sutton et al.,
2007; Thornton et al., 2007; Levy et al., 2008; Sokolov
et al., 2008). These are  highly interactive and nonlinear
systems. The following important interactions are noted:
  Increased deposition of Nr into terrestrial and aquatic
  ecosystems can alter the sequestration of carbon, while
  increased ambient CO2 can change the deposition and
  uptake of Nr.
  Nitrate flux from fields to surface waters increases
  with increasing rainfall (see Box 5:The Impact of
  Climate Change  on Agricultural Discharge of Reactive
  Nitrogen).
  Increasing temperature can both increase and decrease
  atmospheric loading of paniculate matter.
  Aerosols (PM) have  direct and indirect (through cloud
  microphysics) effects on radiative forcing of climate
  and on the hydrological cycle.
  N2O and 63 are greenhouse gases.
• Soil Nr chemistry and emissions of N2O, NH3, and
  NO depend on environmental conditions such as
  temperature and soil moisture.
• The amount of Nr deposited and exported from the U.S.
  depends on meteorological variables including wind
  speeds and convection.
  Numerical models, when verified against past climates.
can provide insight into possible future climates and their
impacts on the nitrogen cycle. For example, increasing
temperatures increase the amount of NOX control
necessary to achieve the same amount of photochemical
smog control (Bloomer et al., 2009; Jacob and Winner.
2009). The EPA program for studying the impact of
climate change on photochemical smog (air pollution
ozone) production offers a useful model; see Jacob and
Winner (2009) for an overview.
  Finding 19: The biogeochemical cycle of Nr is
linked to climate in profound, but nonlinear ways that
are, at present, difficult to predict.  Nevertheless, the
potential for significant amplification of Nr-related
impacts is substantial, and should be examined in more
complete detail.
  Recommendation 19: The EPA  should support cross-
disciplinary and multiagency research on the interactions
of climate and Nr. To determine the interactions of global
biogeochemical Nr cycles and climate, the Committee
suggests that EPA follow a series of steps such as:
1. Select several likely scenarios for global climate from
  the IPCC report for the year 2050.
2. Down-scale statistics or nest regional climate models
  within each of these global scenarios to generate
  meteorological and chemical fields (e.g., temperature,
  relative humidity, winds, precipitation, CO 2) for a few
  years around 2050.
3. Run several independent biogeochemical Nr models
  (earth system models that include air/water/land) for
  North America for these years with current Nr and
  emissions and application rates.
4. Rerun models with decreased Nr emissions/application
  to evaluate strategies for controlling impacts such as
  those described in this report.

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        Box 3: The Impact of Climate Change on Agricultural Discharge of Reactive Nitrogen

   The discharge of reactive nitrogen from intensively managed agroecosystems is characterized by a number of
attributes that often exhibit a high degree of variability: fluctuating material flows associated with the degree of
nitrogen fixation and the extent of denitrification, the interdependence of crops in rotation, and dependence on
geography, weather patterns (particularly rainfall intensity, duration, and frequency), soil type, and agricultural
practices.
   One way to gauge the impact of climate change on such systems is to examine the ranges exhibited by historical
data that collectively encompass the range of impacts that are anticipated. The assumption is that a changing climate
will systematically alter governing attributes in plausibly predictable ways, for example increased annual rainfall
and temperature over a large geographic region. The IPCC has provided general climate-induced impacts for world
regions  (IPCC, 2007a,b).
                                                                      Lognormal Distribution
                                                                      Total NO3 Load from E low
                                                                      (Historical Data
                                     Discharge, Tg/yr
Figure 20: Probability of given discharge level for nitrate in the watersheds of eastern Iowa

Based on the simulation model of Miller et al. (2006). Red markers are historical data of discharges according to
year as reported by Powers (2007). Green bars represent a log-normal distribution.
   The general impact of climate change on the discharge of reactive nitrogen from agroecosystems can be
discerned from the information in Figure 20. This figure shows a probability distribution for nitrate discharged
from the watersheds of eastern Iowa (approximately 50,000 square km), which are dominated by corn-soybean
agroecosystems (a general description of the region can be found in Kalkhoff et al., 2000). It is derived from
information on the input of synthetic fertilizers in the region during the period 1989-1999, and includes factors that
describe the transformation and transfer of Nr once applied. The distribution shown was generated using a Monte
Carlo technique, details of which can be found in Miller et al. (2006). Also included in Figure 20 (in green) is a
standard log-normal distribution, which the simulation most closely fits, and independently measured annual nitrate
runoff data (an output of the system) over the same time period, as reported by Powers (2007). The simulation is
not perfect, but it does capture the extremes of reactive nitrogen discharge, as represented by data for the years 1993
and 1998.
   Figure 20 shows that the interannual variation in nitrate discharged is nearly 30-fold during the 11-year
observation period. While the impact of climate change on such a system cannot be predicted for a given year,
Figure 20 provides a basis for visualizing shifts in nitrate discharge due to changes in those factors that affect Nr
transformation and transfer. For example a climate change scenario that predicts a general increase in precipitation
amount and frequency, other factors being constant, will tend to shift the distribution of Figure 20 to the right,
resulting in generally higher discharges of nitrate (see for example Vanni et al., 2001; the data point for 1993 in
Figure 20 corresponds to precipitation in the region that was approximately 1.8 times the long-term annual average).
Other factors, of course, may amplify or retard such impacts. Understanding whether or not implementation of
best management practices and advanced technological methods can counteract climate change trends that favor
increases in discharge would  require a series of significant research studies and advances in modeling capabilities.

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Integrated   Risk  Reduction  Strategies
for  Nr
5.1. Importance of Integrated Risk
Reduction Strategies
   Chapters 3 and 4 of this report presented the
environmental impacts and metrics associated with the
emission of the various forms of Nr and reviewed ways
of organizing these into impact "categories." As noted,
Nr has many impacts on the environment, impacts
that are interrelated through the nitrogen cascade. As
previously stated, the nature of reactive nitrogen demands
an integrated approach within EPA and across other
relevant federal agencies, as reactive nitrogen cycles
through the environment in different forms. A number
of risk reduction approaches and the importance of
considering Nr control points in the nitrogen cascade are
discussed below.

5.2. Control Strategies for Nr
   There are several ways in which the release and control
of Nr in the environment can be approached. In general
these can be classified as follows:
1.  Improved practices and conservation - in which the
   flux of Nr that creates an impact is lowered through
   better management practices, including those that
   preserve or enhance Nr controlling ecosystem
   services (e.g., on-field agricultural practices,
   controlled combustion conditions, ecosystem function
   preservation and management)
2.  Product substitution - in which a product is developed
   or promoted which has a lower dependency on or
   releases less Nr (e.g., N-bearing wastes instead of
   corn grain as a feedstock for biofuels, development of
   alternative power sources such as wind and solar)
3.  Transformation - in which  one form of nitrogen
   is converted to another form (e.g., nitrification of
   wastewater, denitrification in engineered or natural
   systems)
4.  Source limitation - in which the amount of Nr
   introduced into the environment is lowered through
   preventive measures (e.g., controls onNOx
   generation)
5.  Removal - in which Nr is sequestered from impacting
   a particular resource (e.g., ion exchange)
6.  Improved use or reuse efficiency - in which the
   efficiency of production that is dependent on Nr is
   improved (e.g., increased grain yields for lower Nr
   applied), or Nr wasted from one source is reused in
   another (e.g., algal farming)
   Effective management of Nr requires combinations
of these approaches; none is a perfect alternative for
controlling Nr in the environment. Table 14 provides
a summary of the pros and cons of each of these
approaches.

5.3. Management Strategies for Nr in the
Environment
   Four types of management strategies for the control of
Nr, and other pollutants, in the environment have evolved
over the past 40 years:
1. Command-and-control - in which an entity's
   discharge of pollutants is regulated through a series
   of permitted limitations on emissions, violations of
   which may result in penalties being assessed
2. Government-based programs for effecting a policy,
   such as directed taxes, price supports for a given
   commodity, subsidies to bring  about a particular end,
   and grants for capital expansion or improvement
3. Market-based instruments for pollution control in
   which market trading schemes are used to bring about
   a desired policy end, often at reduced overall cost.
4. Voluntary programs in which desired ends are
   achieved using private or government-initiated
   agreements or through outreach and education.

5.3.7. Command-and-control19
   Policy makers have traditionally used command-and-
control strategies requiring individuals and dischargers
to meet mandatory guidelines. Such an approach evolved
as the country was gearing up to meet the requirements
first established nationally through the CWA and CAA
enabling legislation in the 1970s. Because U.S. capabilities
to monitor contaminant concentrations and predict
environmental impacts were, generally, rudimentary, early
emphasis was placed on "technology-based" approaches
for managing emissions. This resulted in the promulgation
of "best practicable technology" controls, and eventually
"best available technology" controls, the idea being that
mandating some level of control, even with uncertain
improvements on impacts, would be better, and less
arbitrary, than other approaches of the time.
19 Based on Models in Environmental Regulatory Decision-Making, National Research Council, 2007.

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 Table 14 : Advantages and limitations of various approaches to Nr control in forestry and agriculture
^^^^bMjlJjMrajfjUjIikW
Improved practices,
conservation
Product substitution
Transformation
Source limitation
Removal
Improved efficiency
^J
Lessens one or more impacts;
utilization of existing ecosystem
services
Lessens the need for Nr, allows for
more targeted uses of Nr
Reduces one or more impacts
to which Nr contributes, for
denitrification closes the
nitrogen cycle; utilizes natural
biogeochemical processes that may
be available ecosystem services
Reduces one or more impacts to
which Nr contributes
Reduces one or more impacts to
which Nr contributes; natural land
features/processes and ecosystem
services may be used
Reduces the need for Nr

Education cost; availability and cost
of preserved lands
Questions of acceptability,
technological issues
May contribute to other impacts; hu-
man presence has modified and di-
minished ecosystem service values
Decreased crop yields, in some
cases few viable alternatives yet
developed
Residuals containing Nr must still
be managed effectively; availability,
location and cost of land for natural
or enhanced Nr removal
Research and education costs
   Nevertheless, both the CWA and the CAA had
more specific goals that were aimed at protecting
human health, public welfare, and ecosystem health.
For example, the CAA required states to develop
implementation plans (SIPs), the approval of which
depended on their ability, once implemented, to meet
ambient clean air standards. Likewise, the CWA
required greater controls to be implemented for certain
water bodies for which technology-based limits alone
were insufficient to meet standards (this became the
TMDL program).
   Over time, and as our abilities to monitor, predict, and
understand impacts improved, it became possible, or at
least plausible, to tailor emission levels on a source-by-
source basis, allowing the firm in question to decide its
own technological approach. Thus permits, which place
strict limits on the amount of pollution a firm is allowed
to discharge  over a specified period of time, have become
the main method for managing the majority of point
source contaminants, including the various forms of Nr,
in the environment.
   While the CWA has had considerable success in
controlling point source discharges, it has been largely
unsuccessful in limiting nonpoint discharges, and it
is these sources that are particularly important for
managing nutrient flows into receiving waters. The
National Research Council has addressed this deficiency
and pointed to the need to fully implement TMDL plans
and establish numerical nutrient standards for nutrients
(National Research Council, 2008b, 2009).
5.3.2. Government Taxes and Subsidies to
Achieve Policy Ends
   Government taxes and subsidies have created a
variety of results, some in conflict with and some to
further the ends of Nr management. Examples include
U.S. agricultural and land-use policies, energy and
transportation policies, and both point and nonpoint
source mandated  controls on N-bearing aquatic resources,
including domestic and industrial wastewaters and
agricultural runoff.
   Current and future energy policy with respect to
vehicle efficiency and biofuels will help determine
the amount of Nr released into the environment from
these sources. Some states have chosen to place modest
taxes on fertilizer containing Nr, though the demand
impact is slight at best. However, revenues may be
dedicated to improved Nr utilization efficiency. Crop
subsidies and crop insurance may at times expand land
use and even encourage increased use of fertilizers,
effectively increasing Nr in the environment. There
are various agricultural conservation programs in
the U.S. administered by the USD A. These include
the Conservation Reserve Program and the Wetland
Reserve Program (CRP and WRP). The former takes less
suitable land out  of cultivation and the latter encourages
wetland protection and restoration. Both can contribute
to better Nr management. The Environmental Quality
Incentives Program (EQIP) directly subsidizes nutrient
management efforts by crop and livestock producers.
Of concern to the Committee is the need for more

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effective approaches aimed at encouraging farmers
and land managers to adopt proven conservation and
Nr management practices in fields and feedlots. The
extent of proven practices, such as variable rate fertilizer
application and installation of stream buffers, fall far
below today's technological frontier.

5.3.3. Market-Based Instruments for Pollution
Control20
   A fundamental shift in environmental management
philosophy was initiated with the 1990 Clean Air Act
Amendments, which combined regulatory requirements
with market flexibility allowing lower compliance costs
through tradable credits. Most market-based policy
instruments operate on the principle that if the regulatory
framework or some other factor sufficiently alters
the relative value of available decision choices for an
individual or firm, subsequent decisions they make will be
in alignment with the policy makers' objective.
   As an example, if a government wants to limit
pollution in a river where a number of polluters
discharge, it need not adopt a uniform command and
control limit on each discharger. Instead, a regulatory
cap on the total pollutant loadings can be established and
individual permit limits can be issued to all dischargers,
with provisions that allow the dischargers to trade
between their individual limits as long as the overall cap
is not exceeded. Those dischargers having low pollution
control costs will have incentive to control more
pollution than their permit limit and thus generate water
quality credits that can be sold to dischargers with high
costs of pollution control. Because the overall cap on the
pollutant is fixed, the regulatory goal is achieved. Water
quality trading thus brings about the desired reduction in
pollution level at lower cost than if all dischargers were
required to use traditional onsite treatment technology.
Water quality trading also encourages cost-effective
pollution control investment by giving each firm a clear
economic signal to invest in new technology to reduce
pollution at a level that corresponds to the market value
of the permit.
   As with control strategies for Nr, there is no one
universal market-based strategy that is applicable to
every policy maker's objective. For example, the nature
of incentives available to and effective with producers
involved in over-fishing is different from landowners
providing environmental amenities. In the former case,
the objective is to restrict the intensity of fishing. In
the latter case the objective is to encourage private
landowners to provide environmental goods and services
at the lowest cost possible.
   Evolution of new market-based strategies is a
continuous process. Most strategies have been customized
over time to meet local needs. One can group such market
based approaches under the following conceptual headings:
1.  Water Quality Tradable Credits - Every polluting
   entity is allowed to discharge pollutants up to a certain
   pre-determined limit, defined in concordance with
   the terms of the CWA. The entities discharging less
   than their allocated limit generate  credits. Under this
   strategy, credits can be traded with other polluting
   entities that have exceeded their allocated limit
   provided that water quality standards are not exceeded.
2.  Auction-Based Contracting - Environmental or
   conservation contracts are auctioned, where individual
   landowners place their bids to provide such goods or
   services from their land. Two factors jointly determine
   the selection of the bids: the amount of the bid and the
   expected value of the environmental or conservation
   benefit resulting from accepting the  bid.
3.  Individual Transferable Quotas - An individual
   transferable quota (ITQ) is an allocation privilege
   to extract a specified quantity of a resource among
   a selected number of quota holders.  The distinctive
   feature of the ITQ is that the privilege is transferable
   or leasable. An ITQ may be a right to produce under
   favorable circumstances, such as a tobacco quota
   when tobacco production would normally be limited.
4.  Risk Indemnification for Specified Behavior - An
   example of this is crop insurance designed to protect
   farmers from uncertainty in the adoption of best
   management practices that provide a public good but
   are inherently riskier.
5.  Easements - Conservation easements or conservation
   servitudes refer to the case in which a landowner
   enters into  a legally binding agreement to surrender
   certain property rights for a specified period of
   time, either voluntarily or for compensation. Such
   arrangements usually provide public goods relative to
   the environment or conservation.
   Policy maker objective, local conditions, and several
other factors determine the suitability of a particular
market based strategy. For example,  water quality
trading is well suited where there are a variety of
dischargers at  different levels of contribution and with
varying control costs. A policy framework that facilitates
the emergence of multiple options for dischargers to
meet their permit limits, such as buying from more
efficient controllers of discharge or investing in new
equipment to achieve further reductions, is likely to
accomplish the desired level of water quality  at the least
possible cost to the economy. Table 15 illustrates the
potential effective application of a number of market-
based approaches in specific situations. Accompanying
this chapter are two examples of the  application of
 ' Based on Canchi, D., P. Bala and O. Doering, Market Based Policy Instruments in Natural Resource Conservation, Report for the Resource
  Economics and Social Sciences Division, NRCS, USDA, Washington D.C., March 3, 2006, pp. 4-9.

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market-based approaches for the design of water
quality trading schemes for Nr in watersheds (Box 4:
Water Quality Trading to Meet the Long Island Sound
Wasteload Allocation in Connecticut, and Appendix C:
Water Quality Trading in the Illinois River Basin).
   Table 15 shows pair-wise comparison between
different market-based strategies. The objective and
the incentive structure of the participants determine the
suitability of one market based strategy over another.
Each pair of cells briefly lists the most relevant set of
conditions for which the respective strategy may be
optimal (left cell points to strategy at the top of the
column and right cell points to the strategy  at the end
of the row). Consider the two strategies (illustrated
below): Auction-Based Contracting and Tradable
Credit. If the participation of every private entity is
essential, then Auction-Based Contracting works best.
For example, if the objective is to preserve  a large tract
of privately owned contiguous land, Auction-Based
Contracting is the appropriate strategy. This requires
the participation of every private land owner to set
aside a portion of their land. An auction designed to
reveal the individual's land owner's reserve price for
participation leads to the most efficient solution.  A
classic example of this is the Australian Government
of Victoria's auction based Bush Tender program. Here
it was essential to enlist blocks of land with particular
hydrological  and other characteristics to maximize the
reduction in salinity and provide other environmental
benefits. (Department of Sustainability and
Environment, 2008). The goal was to bring  about the
reintroduction of native vegetation,  its protection, and
management  where it would slow the development of
salinity in soils. Offsets would not accomplish this, and
as one looks at Table 15's characteristics of alternative
approaches, neither would quotas, or insurance for
BMPs. Easements might be used, but auctions were
much more cost effective and more  suited to the  long
term management commitment needed, as indicated
in the second row of Table 15 under "Easements."
Compared to this, if the objective is an overall
                     Auction Based
                            xx
                          V
                   When no offsets exist;
                   The participation of
                   every private entity is
                   critical;
reduction of a pollutant regardless of the individual
private entity's contribution to the abatement, the
Tradable Credit strategy with a cap is more appropriate.
As another example, if aggregate depletion is of
concern (as with fisheries) then individual transferable
quotas are appropriate. However, auction-based
contracting is preferable to individual quotas when no
offsets exist.
  Although there are significant differences between
water and air quality trading, there are also several
potential barriers to effective trading systems for
both media. These are related to: accountability and
monitoring; establishing standards and management
goals; complexities of cross media and multiple source
trading, including parity of sources; insurance that
outcomes would reduce risk (environmental benefit);
economics and marketability of traded credits; and
transparency of the program, including public outreach
and stakeholder involvement.

5.3.4. Biophysical and Technical Controls  (con-
trol points) on Transfer and Transformations of
Nr in and between Environmental Systems
  Within the nitrogen cascade there are a number of
places where the flow of Nr is constrained or regulated,
either by nature or by human intervention, or a
combination of the two. This report refers to these places
in the cascade as "control" points. The control points
may restrict the flow of Nr species within environmental
systems (atmospheric, terrestrial, aquatic) or between
them. The control points vary from primary controls
where Nr is minimized through conservation measures or
through after-the-fact measures that attempt to convert Nr
that is emitted or not fully used to nonpolluting products
(such as conversion to N2 by denitrification or through
long-term storage). The discussion of control points in
this section is primarily focused on biophysical controls in
terrestrial and aquatic environmental systems. However,
the section concludes with a discussion of possibilities for
decreasing NOX emissions from combustion.
Offsets are
possible;
Aggregate effect
is of concern, not r
each individual  L
entity's
contribution;
                 Tradable
                  Credit

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   Table 15: Summary of market-based instruments for pollution control with conceptual examples
Auction Based
Contracting



When there
exist no
offsets; The
participation
of every
private entity
is critical;





Offsets are
possible;
Aggregate
effect is of
concern,
not each
individual
entity's
contribution;









Individual
Transferable
Quotas





When the
depletion is of
concern;







Aggregate
depletion is of
concern;








When the
discharge is of
concern;






When there
exist no
offsets; The
participation of
every private
entity is
critical;




Insurance for the
Adoption of BMPs
Homogenous
polluters;
Offsets not
feasible;
Excessive
pollution is
primarily
to mitigate
uncertain
profits;
Modest
short-term
objective;
Tied to a
production
process;
When risk
averseness
of the entity
can be used
to motivate
participation;
Discharge of
effluents is of
concern;

Not
homogenous
polluters;
Offsets are
possible;
Pollution is
an absolute
consequence
of the
production
process;

Not tied to any
production
process;
Suited for
motivating
participants
to engage in
secondary
activities;
Depletion of a
resource is of
concern;




Easements

Unidirectional;
When offsets
are not
possible; One
entity retiring
more property
rights cannot
trade with the
other retiring
less property
rights.

Auction based
contracting
can bs sssn
as a rsfinsd
and improved
cost~sfficisnt
altornativo to
sassmsnts'

Retirement
of rights is of
concern;
No uncertainty;
No action
required on
the part of the
participant;


Bidirectional;
Offsets are
possible;
Requires
specific action
on the part of
the participant
to accomplish
the objective;



Designing of
auction based
contracting
requires
considerable
professional
expertise;

Acquisition
of rights is of
concern;

Tied to a
production
process:







Trad able
Credit








Auction
Based
Contracting



Individual
Transferable
Quotas
Insurance

for the
Adoption of
BMPs

Each pair of cells briefly lists the most relevant set of conditions for which the respective strategy may be optimal (left
cell points to strategy at the top of the column and right cell points to the strategy at the end of the  row).
   Box 4: Water Quality Trading to Meet the Long Island Sound Wasteload Allocation in Connecticut
     Pollutant trading is increasingly being promoted as a cost-effective means for attaining water quality standards.
  Connecticut and New York have been working with the EPA Long Island Sound Study (LISS) for more than
  20 years to address low oxygen conditions (hypoxia) in Long Island Sound that have been linked to excessive
  loadings of nitrogen. A Total Maximum Daily Load (TMDL) for nitrogen, drafted by the two states and approved
  by the EPA in 2001, set a 58.5% nitrogen reduction target in 2014 from point and nonpoint source/stormwater
  sources. Connecticut has initiated a point source trading program for 79 municipal sewage treatment plants (STPs)
  to facilitate implementation of the TMDL wasteload allocation (WLA) and is investigating the potential for
  incorporating nonpoint source/stormwater into the existing Nitrogen Credit Exchange (NCE).
     Several prerequisite conditions essential to the success of the current point source trading program have been
  met. Briefly, (1) all the STPs contribute to the same water quality problem; (2) the technology to remove N and meet
  the targets exists; (3) there are compelling member benefits to participate, especially cost savings; (4) sources can
  easily be monitored and tracked by end-of-pipe monitoring; (5) credit cost calculations are based on established and
  agreed upon protocols founded in state legislation; (6) sources of N are diverse and create viable supply and demand
  conditions while reducing overall cost, with close control by a Nitrogen Credit Advisory Board (NCAB); and (7)
  transaction costs are low relative to credit prices. In operation since 2002, the NCE has proven to be a viable and
  effective mechanism for meeting the nitrogen WLA.
     The economic record of the NCE demonstrates the vigor of trading over the first five years of completed trades
  from 2002 to 2006 (Table 16). In sum, more than 10 million credits have been traded on the NCE, representing more
  than $22 million in economic activity.

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   The use of geographically-based trading ratios is instrumental to the relative cost of meeting N reduction limits at
the 79 treatment plants, which are scattered throughout the state (Figure 21). Because N is reactive as it travels down
rivers into the Sound, and the Sound's currents further affect relative impacts as they transport N and the resulting
algal blooms to the hypoxic areas at varying efficiencies, location of each treatment plant makes a difference in
relative impact on dissolved oxygen per pound of N discharged at end of pipe. Generally, the closer a POTW is to
the edge of the Sound, and the closer to the hypoxic zone, the higher the trading ratio (Figure 22). For plants with
high trading ratios, economics often favor treatment, while those with lower ratios may find the purchase of credits
economically advantageous over treatment.

                       Table 16: Performance of the Nitrogen Credit Exchange
         2002
         2003
         2004
         2005
         2006
          Total
$1.65
$2.14
$1.90
$2.11
$3.40
$1,317,223
$2,116,875
$1,786,736
$2,467,757
$3,828,114
$2,357,323
$2,428,636
$2,659,804
$1,315,392
$2,394,956
             $11,516,705     $11,156,111
  798
  989
  940
1,170
1,126
                                   5,023
1,429
1,135
1,400
  623
  704
                                 5,291
     Source: Connecticut Department of Environmental Protection, 2007
                                           STP Nitrogen Loads
                                            N Load llbs/dl
                                           • 0-200
                                           • 200 - 600
                                           • 600 - 1300
                                           • 1300 - 2900
                                           • 2900 - 6500
                  Figure 21: Relative nitrogen discharge (Ibs/day) from 79 POTWs.
                  Source: Connecticut Department of Environmental Protection, 2007
   The point source NCE does not reflect a free market approach to trading. Demand is set by the annual general
permit limit and supply of credits is constrained by the availability of Clean Water Fund dollars and the timing and
location of N removal projects. Credits are bought and sold from the state, thus the number of credits purchased does
not need to match the number of credits sold (as would typically be true in a tradable permit system). Nevertheless,
there is a tendency towards implementing cost effective projects as sewage treatment plant authorities decide
whether it is less expensive to treat or buy credits, and try to predict when that break-even point might occur that
would warrant application for project funding.

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                                             Nitrogen Trading Zones
                    Figure 22: Trading ratios for municipalities in Connecticut.
                    Source: Connecticut Department of Environmental Protection, 2007

   Incorporating a nonpoint source/stormwater (NPS/SW) component into the existing point source trading program
presents some difficult challenges. Among the seven prerequisite conditions listed above that are well met by the
current point source program, NPS/SW trading does not provide compelling economic benefits for members; NFS/
SW N is difficult to quantify and track; credit cost estimation does not have a strong foundation in any existing
programs; NPS/SW credit costs, though geographically diverse, may not result in significant implementation
savings; and transaction costs (or time spent negotiating the ground rules for NPS/SW trading) may be considerably
higher than for point source credits. Many of these obstacles can be overcome by deferring to models and textbook
costs and efficiencies for NPS/SW BMPs. Tracking will still be a challenge because of the sheer number and
distribution of BMPs that can be applied throughout the state.
   Basic economic principles suggest that a free-market arrangement will not produce many NPS/SW credits for
market. Costs are much higher than for point source credits and a regulatory approach must therefore be instituted to
formalize the load allocation for nitrogen and to structure participation by municipalities.
   If a NPS/SW trading component were to be added in the future, it would most likely also be an incentive-based
program rather than a free-market approach. Nitrogen is difficult and costly to control in Connecticut's urban/
suburban setting, and reductions are unlikely to be cost- competitive with POTW credits in a free market system.
However, because municipalities are required to implement the Phase II stormwater permit, and various federal,
state, and local programs require or emphasize NPS/SW management, there may be benefits for an incentive-based
approach to offset some of those costs. For example, payment for NPS/SW reductions at the same credit prices paid
to POTWs under the NCE would help defray costs and encourage additional nitrogen reductions from stormwater/
NPS sources. Connecticut and the NCAB will continue to evaluate and explore the viability of these options.
   Market approaches and trading can lower costs and increase economic efficiency of Nr control. The approaches
may well have to be situation specific and depend on a structured regulatory framework to create the market or
trading opportunity. As with the 1990 Amendments to the Clean Air Act, the design of market based instruments
is a product of technical capability, regulatory design, and public preference. Implementation can be tedious but
the benefits in efficiency  substantial, even after being balanced against equity concerns. However, there can be
something of a geographical and supply/demand mismatch between nonpoint sources and point sources that might
be trading partners. Ribaudo and Nickerson identify only  142 of 710 eight-digit Hydrologic Watershed Units
containing waters impaired by nitrogen where trading would be most likely (Ribaudo and Nickerson, 2009).
   Further, the cost for management will be enormous. EPA's Clean Water Needs Survey (EPA, 2008b) has identified
more than $200 billion in wastewater management infrastructure needs, and those needs do not fully address nutrient
control from both traditional point and nonpoint/stormwater sources or consider alternative technologies.

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Biophysical controls in terrestrial environmental
systems
   As indicated in Figure 2, approximately 36 Tg of
new Nr is introduced into the U.S. each year. This new
Nr is derived from sources that include consumption of
-11 Tg of synthetic N fertilizer, ~8 Tg of N that is fixed
biologically by crops, and ~ 5 Tg that is emitted from
fossil fuel combustion annually. This N is used to produce
food and fiber (~15 Tg) or is formed during electrical
generation, industrial production, or transportation.
Efforts to decrease the creation of new Nr should first
look to conservation.
   Reduction in use of fossil fuel and/or decreased Nr
emission can come through a variety of mechanisms such
as more energy-efficient industrial processes, homes, and
vehicles. Further gains are possible through conservation
practices and alternatives to wasteful approaches, such
as improving public transportation to minimize use of
personal automobiles, and use of local products that do
not require long-distance shipping.
   Improvements in food and fiber production and
changes in diet can also play an important role in limiting
Nr. Because agriculture is the largest consumer and
producer of Nr, consumption of fertilizer N could be
decreased by changes in diet and increasing fertilizer N
use efficiency in crop and fiber production systems. The
control points discussed in this section include: protein
consumption in the human diet; removing croplands that
are highly susceptible to Nr loss from crop production;
decreasing fertilizer N demand by increasing fertilizer
use efficiency in crop and fiber production, as well as
on residential and  recreational turf grass; and better
management of Nr in manure from livestock production
in CAFOS.

Decreasing the  amount of fertilizer N needed
through changes in human diet
   Along with increasing fertilizer N use, continued high
intake of protein in developed countries and changes in the
diet of people in developing countries will likely lead to
greater N losses from global food production in the future.
The first aspect of changes in food production concerns
the increasing protein consumption that is occurring as
global population increases and gets  wealthier. This is
likely to require increased N input into food production
(Naylor et al., 2005; Galloway et al., 2007).
   The average protein supply per person in developed
countries is presently -100 g per day, while in the
developing countries it is only ~65 g per day (Food and
Agricultural Organization Statistical Database (FAO
FAOSTAT, 2010a). There is a direct proportionality
between protein and nitrogen composition of food (ca
0.16 g N per 1 g protein). On average in 1995, developed
countries consumed -55% of total protein from animal
sources while developing countries derived -25% of total
protein from animals. Protein consumption was highest
in the U.S. and Western Europe, -70 and -60 g animal
protein per person per day, respectively. In 2003, total
protein consumption in the U.S. was 115 g per person
per day (74 derived from animals and 41 from vegetable)
(FAO FAOSTAT, 2010a). In developing countries,
the greatest change in animal protein consumption
has occurred in China where the consumption of meat
products has increased 3.2 fold (from -10 to -32 g per
person per day) since 1980. In Sub-Saharan Africa there
has been no increase in either total (-50 g per person
per day) or animal protein (-10 g per person per day)
consumption during the past 30+ years (Mosier et al.,
2002).
   The reason for focusing on the consumption of
animal protein is that more N is needed to produce a
unit of animal protein than an equal amount of grain
protein. Bleken et al. (2005)  note that the N cost of
animal production in Norway and the Netherlands was
approximately five units of N in feeds for each unit of
N produced. Approximately 2.5 units of N are required
to produce a unit of wheat protein-N. Bequette et al.
(2003) report that dairy cattle consume four units of N
in feeds (including forage and grains) for every unit of
N that appears in milk. Using a range of efficiencies
for animal production practices, Kohn et al. (1997)
estimated that 4 to  11 units of fertilizer N would be
used in a whole farm system to produce a unit of milk
protein. This ratio would be lower when using legume
N to feed cattle, as is commonly done. Based upon the
extra N required to produce animal protein compared
to grains, continued high protein consumption in
developed countries and changes to higher protein diets
in developing countries will likely increase N input and
losses in food production.
   Moderating this increase by decreasing the average
amount of total protein consumed in developed countries
is one mechanism of limiting  part of the expected
increased N requirement in food production. One
example of a country with a healthy diet and moderate
consumption of animal protein is Italy in 1963. At that
time, food supply was adequate to ensure sufficient
nutrition to all groups of society (Bleken, 1997). Total
protein consumption was 85 g per person per day, and
consumption of animal protein was 32 g, roughly half
of the current U.S. diet, and yet much higher than the
average of developing countries. Another example is
Japan, where animal protein consumption has traditionally
been low, although it has increased from 25 g in 1963 to
54 g animal protein per person per day in 1995. In the
same period the total protein consumption has increased
from 73 g to 96 per person per day.
   Bleken (1997) analyzed the relation between human
diet and global N need for food production. Her analysis
indicates that the total N needed for diets with high
animal protein intake (comparable to many industrialized
countries today) is almost twice as high as the N needed

-------
for the average diet in Italy 1963, or for Turkey in 1993.
Based on her analysis, the Committee assumes that in
the high-N input regions, per capita N need for food
production may be reduced by 45%, which would reduce
present-day N inputs by 15% worldwide.
   Switching to a lower protein diet may not, however,
reduce N losses if the new diet includes increased
quantities of fruits, vegetables, and nuts, in addition to
staple grains, beans and pulses. Vegetables, fruit, and
nuts are high value crops that typically require large
inputs of fertilizers and pesticides when produced at a
large, commercial scale, and N fertilizer losses can be
considerably larger than for grain crops. Having a very
diverse diet that includes a wide range of high-value
fruits and vegetables available year round (whether
they are in-season locally or not) also has consequences
for N inputs/outputs from agriculture - both within the
U.S.  and globally. EPA and USDA are encouraged to
develop programs that stress how both human health and
environmental health will improve with a greater focus on
the human diet.  It has been estimated that 30% - 40% of
the food prepared for consumption in the U.S. is wasted
(Kantor et al., 1997; Hall et al., 2009). Thus, additional Nr
may be conserved by decreasing the amount of food that
is wasted.

Removing croplands that  are susceptible to Nr
loss from crop production
   An analysis of NO3~ loading in the Mississippi
River Basin (Booth and Campbell, 2007) provides
estimates of N input from agricultural lands. Similar
estimates were provided by Del Grosso et al. (2006).
Recommendations in this analysis are essentially the
same as those arrived at in the original national hypoxia
assessment, which suggested  that the most leaky lands
be taken out of production (Doering et al. 1999). Booth
and Campbell state:
   Nitrogen derived from fertilizer runoff in the
   Mississippi River Basin (MRB) is acknowledged as
   a primary cause of hypoxia in the Gulf of Mexico.
   To identify the location and magnitude of nitrate
   runoff hotspots, and thus determine where increased
   conservation efforts may best improve water quality,
   we modeled the relationship between nitrogen inputs
   and spring nitrate loading in watersheds of the MRB.
   Fertilizer runoff was found  to account for 59% of
   loading, atmospheric nitrate deposition for 17%,
   animal waste for 13%, and  municipal waste for
   11%. A nonlinear relationship between nitrate flux
   and fertilizer N inputs leads the model to identify a
   small but intensively cropped portion of the MRB
   as responsible for most agricultural nitrate runoff.
   Watersheds of the MRB with the highest rates of
   fertilizer runoff had the lowest amount of land enrolled
   in federal conservation programs. Our analysis
   suggests that scaling conservation effort in proportion
   to fertilizer use intensity could reduce agricultural
   nitrogen inputs to the Gulf of Mexico, and that the
   cost of doing so would be well within historic levels
   of federal funding for agriculture. Under this simple
   scenario, land enrolled in conservation programs
   would be increased by about 2.71 million hectares, a
   29% increase over 2003 enrollments, while land taken
   out of traditional fertilized agriculture and enrolled in
   conservation programs would constitute about 3% of
   2003 fertilized hectares.
   The Booth and Campbell approach places the leakiest
intensively cropped lands into government programs
like the Conservation Reserve Program - where they
would be put into grass or cover crops. Doering et al.
(1999) had a somewhat different approach. Under their
analysis, nitrogen use or nitrogen loss reductions were
imposed on agriculture, and the U.S. Agricultural Sector
Mathematical Programming (USMP) model adjusted
crop rotations, tillage practices and fertilizer inputs
within the Mississippi Basin - meeting the given Nr
constraint while maximizing producer and consumer
welfare. The model favored those crops and cropping
systems at different points in the landscape having low
nitrogen leakage. Where the model could not find a crop
production system having positive returns while meeting
the Nr restrictions, the land was retired from production.
This analysis suggests opportunities for maintaining land
in agricultural production while still reducing Nr losses
through better matching of land characteristics with crops
and cropping systems.
   This  1999 analysis of the Mississippi Basin was
carried out in the context of cost effective approaches -
starting with the most cost-effective (in terms of producer
and consumer welfare) and moving to less cost-effective
approaches as more and more nutrients were controlled.
This included both restriction of fertilizer inputs, buffers,
and wetland remediation as well as the land use changes
and crop rotations referred to above. The suggestions
presented by the Committee for Nr reductions that could
be achieved from agriculture with existing technology
are consistent with the cost effective approaches in the
1999 Hypoxia Assessment's economic analysis. Cost
effectiveness and alternative cropping systems were
considered in the SAB report, Hypoxia in the Northern
Gulf of Mexico: An Update by the EPA Science Advisory
Board (U.S. EPA SAB, 2007) but unfortunately as
pieces from individual study examples rather than as an
integrated approach like the 1999 Hypoxia Assessment
(Doering etal., 1999).
Decreasing fertilizer N demand by increasing
fertilizer use efficiency in crop and fiber production
   The largest input of Nr in North America is N
fertilizer used for crop production. The mean annual
N fertilizer input to North America between 1999 and
2003 was  12.5 Tg. Of this fertilizer N, 66% was used to
fertilize cereal crops,  mainly corn and wheat (Dobermann
and Cassman, 2005).

-------
   As previously discussed, corn yield in the U.S. has
increased (from an average of 100 bushels per acre in
1985 to 136 bushels per acre in 2005) as a result of
improved nutrient and pest management, expansion
of irrigated area, conservation tillage, soil testing, and
improved crop genetics (yield and pest resistance)
(Council for Agricultural Science and Technology
[CAST], 2006). From 1980 to 2000, N-fertilizer use
efficiency (NFUE, kg grain produced per kg applied N,
or kg grain / kg N) increased from 42 to 57 kg grain / kg
N, a 35% efficiency gain during a period when average
U.S. corn yields increased by 40% (Fixen and West,
2002). Despite this steady increase in NFUE,  the average
N fertilizer uptake efficiency for corn in the north-central
U.S. was 37% of applied N in 2000 based on direct
field measurements (Cassman et al.  2002). These results
indicate that greater than 50% of applied N fertilizer
is vulnerable to loss pathways such as volatilization,
denitrification, runoff, and leaching. The results also
suggest there is substantial room for improvement in
N efficiency currently achieved by farmers. Although
progress has been made to increase  both cereal yield
and NFUE, a concerted effort to further increase NFUE
remains a logical control point to reduce production
costs, because N fertilizer represents a significant input
cost, and to limit Nr leakage (e.g., NHs, NOX, N2O,
NCV) from agroecosystems.
   The goal of reducing Nr while sustaining adequate
rates of gain in cereal production to  meet expected
food demand will require increases in NFUE, which in
turn will require innovative crop and soil management
practices. This need is exacerbated by the recent increase
in demand for corn to produce ethanol biofuel. The
concept of improved N synchrony (practices that better
match the amount, timing, and geospatial location of
applied N to crop-N demand and the N supply from
indigenous  soil resources) is generally viewed as the
most appropriate approach for improving NFUE (e.g.,
Appel, 1994; Cassman et al., 2002). The challenge is to
attain greater synchrony between crop N demand and the
N supply from all sources (e.g., soil, fertilizer, organic
inputs such as manure, compost, or green manures)
throughout the growing season. Losses from all N-loss
mechanisms increase in proportion to the amount of
available N present in the soil profile at any given time.
   Several promising technologies and combinations of
technologies have emerged in recent years. Significant
increases in NFUE are often achieved through reducing
N fertilizer use by 10 to 30%,  while still maintaining
or even slightly increasing yields (Giller et al. 2004).
Figure 23 indicates where the  greatest gains in NFUE
are expected to be realized from investments in different
technology options. Improvements in crop and soil
management practices will contribute to higher NFUE
by achieving greater congruence in timing of the supply
of applied N with crop-N demand and the N supply from
                             -  ""    Genetic engineering

                             Plant and strain
                             selection and breeding
                  Improved agronomic management
                  Existing Knowledge
                   Titncscale
Source: Giller et al., 2004 (Figure 3.2, p. 48). Reprinted
with permission from Island Press; copyright 2004,
SCOPE.
indigenous soil resources. While there is relatively small
scope for specific biotechnology traits to improve NFUE,
overall improvement in crop genetics from commercial
breeding efforts that focus on increasing yield and
yield stability will continue to play a significant role in
improving overall NFUE. However, large investments
in research, extension education, and technology
transfer will be required, and significant incentives must
be implemented, to achieve the degree of improved
synchrony needed to  make substantial improvements
in NFUE. The need to accelerate the rate of gain in
crop yields to meet increasing demand for human food,
livestock feed, and biofuels represents an additional
new challenge. Crop  prices  are expected to rise as they
more closely track the price of petroleum (Council for
Agricultural Science  and Technology, 2006). Higher crop
prices will motivate farmers to achieve higher yields, and
higher crop yields require a greater amount of N uptake
to support increased biomass production (Greenwood et
al.,  1990). Therefore, an explicit emphasis on developing
technologies that contribute to both increasing yields
and NFUE will be needed to ensure that the goals of
food security, biofuel production, and protection of
environmental quality are met.

Alternatives to  current urban landscaping practices
   Section 2.2.4 discussed the use of turf grasses as a
prominent feature in U.S. urban landscapes with over
1 TgN used to fertilize lawns each year (Table 9).
New developments are most amenable to landscaping
practices that may minimize the need to use supplemental
fertilizer. These practices include preservation of the
natural soil profile, use of turf types that  require little
or no fertilizer, minimizing turf areas, using organic

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maintenance techniques, and choosing alternatives
to lawns and exotic plant species such as naturalistic
landscaping. Many of these practices are part of a low
impact development philosophy, which can also combine
other best management practices to mitigate the effects
of impervious cover and landscape changes. Existing
development is also amenable to many of these practices,
especially conversion of typical residential
and commercial lawns to natural landscapes and
retrofitting other BMPs that promote infiltration, such as
rain gardens.

Structural and non-structural Best Management
Practices (BMP) to treat runoff
   There are probably hundreds, if not thousands, of
BMPs that have been designed and manufactured to treat
runoff from both urban and agricultural lands. Whether
applied to new development or existing agricultural or
urban land use, most follow basic principles that simulate
natural land features and processes that remove pollutants
from runoff. They promote infiltration to take advantage
of the cleansing value of passage through soils and to
reduce runoff volumes, and provide for biological or
chemical conditions that help remove pollutants (NRC,
2008b, 2009).
   The most notable of the processes for managing Nr is
providing conditions that are adequate to denitrify Nr in
the waste-stream in a process called biological nitrogen
removal (BNR). BNR simply creates conditions that
convert initial forms of nitrogen to nitrate via oxidation,
and convert nitrate to dinitrogen gas by providing
conditions (especially high carbon and low oxygen)
where the denitrification process can occur. These
simulate natural conditions such as nitrification that
occurs  in oxic soils as water-borne nitrogen infiltrates into
the soils and groundwater, and denitrification that occurs
in highly-organic, saturated soils such as in wetlands
where oxygen is low.
   Most BMPs are considered structural, and may
be highly engineered "package" plants that can treat
sewage or runoff, depending on scale and structure,
or simple detention basins that allow sediments and
adhered pollutants to settle out. "Artificial" wetlands are
a good example of a more sophisticated BMP that takes
advantage of natural processes, and may be created at the
end of the stormwater pipe, or at edge of field. Structural
BMPs are an important part of any strategy to limit
reactive nitrogen loss to the environment. For example,
The State of Iowa contains some of the most productive
agricultural land in the world. Of the 36 million acres of
land, 23 million acres are planted in corn or soybeans.
Approximately 39 percent of the corn/soybean acres
are drained with an estimated 800,000 miles of tile
(Cutler, 2000). Each year, thousands of miles of new or
replacement tile are installed. This drainage network is
responsible for the conveyance of 90 percent (Crampton
et al., 2006) of the nitrate that appears in Iowa's surface
waters. Control of nitrogen discharge from drainage
tile will be needed to limit reactive nitrogen loss to the
environment from agriculture.
   Various approaches have been proposed with varying
degrees of success. Constructed wetland, bio-reactors,
and drainage tile encapsulated with biomass have
proven to reduce 90 percent of the reactive nitrogen
loss to the environment (Blowes et al., 1994). It would
seem reasonable to require any new or replacement
drainage tile to implement a control strategy at the time
of construction and a retrofit program for the remaining
drainage systems. With an average of two years to pay
back the cost of drainage systems due to increased crop
yield, the added cost for nitrogen control does not seem to
be unreasonable.
   Non-structural BMPs are often preservation actions,
as discussed earlier, or activities that prevent pollutants
from entering the waste stream such as street sweeping or
fertilizer limitation.

Engineered and restored wetlands to decrease
NO3~loading of aquatic systems
   The construction and/or restoration of wetlands have
received considerable attention in the past two decades
as a conservation method.  Such an approach has several
positive attributes including promoting denitrification in
watersheds containing or receiving Nr, flood protection,
habitat preservation, and recreational potential (Hey and
Philippi, 1995). In the upper Mississippi basin optimum
siting of wetlands could result in as much as 0.4Tg of NOs"
converted to N2 (Mitsch et al., 1999, 2001; Hey, 2002).
   Much of the nitrate leached from agricultural fields
could be removed from drainage water in natural,
created, or restored wetlands. Nitrate removal from the
water column in wetlands is performed by plant uptake,
sequestration in the soils,  and microbial  transformation
that includes immobilization and denitrification. Plant
uptake and microbiological immobilization result in
temporary storages in the  system since most nitrogen
will eventually return to the wetland via plant death and
decomposition. In contrast, denitrification can constitute
a real nitrogen sink because NCV is converted mainly
to N2 that is emitted to the atmosphere (Clement et al.,
2002). As discussed in Section 4.7, the potential for the
formation of N2O is of concern if such systems are not
operated properly.
   In addition to preserving existing wetlands, there are
two other basic approaches that utilize wetlands to reduce
the Nr and other nutrients reaching rivers, streams, and
vulnerable downstream coastal systems.  These  approaches
are: 1) creation and restoration of ecosystems, principally
wetlands and riparian forests, between farms and adjacent
ditches, streams and rivers; and 2) diversion of rivers into
adjacent constructed and restored wetlands all along the
river courses (Mitsch and  Jorgensen, 2004; Mitsch and
Gosselink, 2007).

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   The Committee notes that if wetlands can be
economically and effectively restored where croplands
now exist on hydric soils within the 100-year floodplain,
this may be an important NO3~ control mechanism.
Cropland on hydric soil in the floodplain occupy about
6.9 million acres (2.8 million hectares). If this area and its
wetlands were given back to the Mississippi River, over
a million tons of NO3" -N would be annually removed or
prevented from reaching the Gulf of Mexico (Mitsch et
al., 1999; Hey, 2002; Hey et al., 2004). To give scale to the
solution needed, restoration of over 4.9 million acres (2
million hectares) of wetlands is needed in the Mississippi-
Ohio-Missouri (MOM) river basin to reduce the nitrogen
load to the Gulf of Mexico sufficiently to ensure a
reduction in the size of the hypoxic zone in the Gulf of
Mexico (Mitsch et al., 2001).
   At a series of workshops on restoration of the MOM
river basin in 2003-2004 (Day  et al., 2005; Mitsch and
Day, 2006), scientists and managers were asked to
focus  on needed research and chokepoint opportunities
for managing N in that basin. They concluded that a
major, interdisciplinary research program (as a lead-
in to the actual restoration of wetlands and rivers) was
needed, with sufficient funding, study sites, and time
to reduce remaining uncertainties about the efficacy of
wetlands to solve pollution problems related to N. It
was recommended that, to implement this program, 20
to 30 full-scale, existing and new agricultural/wetland
demonstration projects should be located throughout the
country and instrumented to study agricultural runoff into
wetlands in a variety of soil conditions. Pilot and full-scale
studies of diversions into riparian systems along river
channels were recommended in order to determine the
effectiveness of these systems for nutrient removal. The
Committee notes that these research and demonstration
projects have not been undertaken, and that there is a
continuing need for this work.
   Further illustration of the use of wetlands as a tool for
Nr management tool is presented in Appendix C, Water
Quality Trading in the Illinois River Basin (D. Hey,
personal communication).
Technical controls (control points) on transfer
and transformation of atmospheric emissions of
Nr in and between environmental systems: NH3
   Newly fixed Nr is produced biologically or added as
fertilizer to meet the demand for food and fiber production.
Much of the N is used in cereal crop production and cereal
crops  are then used to feed livestock. The new Nr is then
recycled through the livestock  production system where
it becomes again susceptible to losses to the atmosphere
as ammonia and NOX, and is available for additional N2O
production and movement into aquatic systems as NH4+
and NO3-.
   The bulk of the N fed to livestock ends up in manure,
and where this manure (approximately one-half in urine
and one-half in feces) is produced, there is often a much
greater supply than can be efficiently or economically
used as fertilizer on crops. For large concentrated animal
feeding operations there is considerable expense associated
with disposal of the manure. Various storage systems have
been developed to deal with this excess manure, the most
interesting of which, from the standpoint of integrated
policy on N, convert the urea to N2. The fraction of
manure N that can be and is converted to N2 remains a
major unanswered scientific or technical question.
   The NRC (2003) noted the paucity of credible data on
the effects of mitigation technology on rates and fates of
air emissions from CAFOs. The report did, however, call
for the immediate implementation of existing atmospheric
emission technology. The NRC (2003) also called for a
mass balance approach in which the losses of N species
such as NH3, NO, N2, and N2O are expressed as a fraction
of the total N loss. Quoting from the NRC report:
   Storage covers for slurry storage tanks, anaerobic
   lagoons, and earthen slurry pits are being studied
   as a method to decrease emissions from those
   containments. Both permeable and impermeable
   covers are being studied. Tested covers range from
   inexpensive material such as chopped straw (on slurry
   containments only) to more expensive materials such
   as high density polyethylene. Covers can decrease
   emissions from storage but their net effect on
   emissions from the system is conditional on how the
   effluent is used on the farm.
   Anaerobic digestion in closed containment has been
   studied for many types of applications. Anaerobic
   digestion is the process that occurs in an anaerobic
   lagoon. When conducted in closed vessels, gaseous
   emissions including methane, carbon dioxide and
   small amounts of other gasses (possibly ammonia,
   hydrogen sulfide, and VOCs) are captured and can be
   burned for electricity generation, water heating, or
   simply flared.  The in-ground digester being tested on
   a swine farm in North Carolina is an example of the
   ambient temperature version of this technology (there
   are also mesophilic and thermophilic designs). The
   concentration of ammonia remaining in effluent from
   that digester is higher than the concentration in lagoon
   effluent and can be volatilized once exposed to air.
   Recent research (e.g., Bicudo et al., 2004; Funk et al.,
2004a,b; Shores etal., 2005) demonstrates reduction in
NH3 emissions after a permeable cover was installed.
Miner et al. (2003)  reported that a polyethylene cover
can reduce NH3 emissions by -80%, but it is not clear
what fraction of that N was converted to N2. Harper et
al. (2000) reported that in a well-managed swine lagoon
denitrification N2 losses can be equivalent to N lost as
NH3, in other words about 50% efficiency. Kermarrec
et al. (1998) reported that sawdust litter helped reduce
NH3 emissions from pig manure with 44-74% of manure

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N converted to N2, but greater than 10% of the manure
N was released as N2O. Sommer (1997) found that NH3
was emitted from cattle and pig slurry tanks at the rate
of 3.3 kg N m^r1 until covered with straw. After straw
application NH3 emissions were below detection limit.
Mahimairaja et al. (1994) reported that NH3 volatilization
was reduced by 90-95% under anaerobic conditions.
Section 2.2.4 contains a discussion of best management
practices to minimize NH3 emissions from livestock
waste, and presents finding and recommendation 6 on the
need for a framework for manure management.

Technical controls (control points) on transfer
and transformations of atmospheric emissions of
Nr in and between environmental systems: NOX
  As previously discussed, a major contributor to Nr
in the atmosphere is fossil fuel combustion. During the
combustion process NOX (NOX = NO + NO2) are released
to the atmosphere. Globally the production of NOX has
accelerated in the last few decades, primarily through the
increase in fossil fuel combustion (Galloway et al., 1995;
2008). With this increase in emissions from ~5 Tg N in
1940 to ~ 25 Tg N in 2005, combustion of fossil fuels
accounted for about 50% of the total global NOX  emissions
for 1990. Of the anthropogenic sources, fossil fuel,
aircraft, biomass burning, and part of the soil emission are
most important (Holland et al., 1997). Although global
NOX emissions continue to increase, these emissions are
declining in the U.S. (see Section 2.2.1).
  Nitrogen oxide is formed during combustion by three
mechanisms:
  Thermal NOX where N2 and O2 combine at high
  temperatures (thermal pathway dominates at
  temperatures greater than approximately 1500  C) to
  form NO through the Zeldovich mechanism
  Fuel NOX where nitrogen from a fuel (e.g., coal and
  biofuels) is released as some intermediate and then
  combines with O2 to form NO
  Prompt NOX where N2 reacts with hydrocarbon radicals
  in flames, forming various compounds including
  hydrogen cyanide and other cyano radicals. These
  in turn form NOX. Contributions of prompt NOX are
  usually low as compared to fuel NOX.
  There are several ways to control emissions of NOX.
The most common controls are on coal-fired electric utility
generators and those are discussed below. Following the
discussion of electric utility generator controls, or external
combustion systems, there is a discussion on internal
combustion controls.
   Reduction of the temperature limits the kinetics of
the N/O2 reaction. Temperature can be controlled by
using a fuel-rich mixture versus fuel lean. In this case
the reactions take place at lower temperatures. Fuel-
rich mixtures also reduce the amount of O2 available
for reaction and there are changes in the chemical
mechanisms which limit the oxidation of N2. If fuel-
lean mixtures are used for temperature control, while the
temperature is lower there is a significant amount of O2
present. Typically, in external combustion systems controls
are implemented by using less excess air and using staged
combustion. In addition, flue-gas recirculation (FOR) is
used to lower the temperature. Low-NOx burners operate
under the principle of internally staging the combustion.
To reduce fuel NOX, air and fuel staging are used to reduce
the peak temperature where air and fuel are admitted in
separate locations.
   Chemical reduction of NOX is also possible. These
methods include: selective non-catalytic reduction
(SNCR), selective catalytic reduction (SCR), and fuel
reburning. SNCR is an add-on technology where urea or
NH3 is injected in a controlled temperature zone to allow
the reduction of NOX. SCR  is  also an add-on technology
where the flue gas must pass through a catalyst bed to
allow reaction between ammonia and NOX. Care must
be taken with both technologies to avoid NH3 slip. Fuel
reburning requires the injection of a fuel to create a zone
where NOX is reduced to N2. Low NOX burners may also
use an internal fuel reburning to reduce the NOX.
   For internal combustion  engines, the same mechanisms
discussed above are used in a variety of different
ways, since these systems are using high pressure and
predominately have thermal NOX versus fuel NOX
formation. Most technologies involve the need to reduce
the peak temperature and duration of high temperatures
of the combustion zone. For example, gas turbines utilize
low NOX burners, while spark ignition engines utilize a
three-way catalyst which requires less than 0.5% O2. In
this case, additional NOX is reduced by utilizing unburned
fuel as a reagent over the catalyst for chemical reduction
of NOX. It should be noted,  however, that a side reaction
for the three-way catalyst system produces ammonia. NOX
emissions can be reduced in diesel engines by delaying the
injection of the fuel, and by retarding the timing in spark-
ignited engines.  Engines also use exhaust gas recirculation
(EGR) to reduce the peak temperatures. Recent road side
studies have indicated high efficiency (-90%) for NOX
removal from the American light-duty fleet (Bishop and
Stedman, 2008).

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   This concluding chapter contains the Integrated
Nitrogen Committee's findings and its recommendations to
EPA. Section 6.1 discusses the need for a comprehensive
program to monitor reactive nitrogen. Section 6.2 provides
the Committee's overarching recommendations to EPA.
Section 6.3 contains suggestions for near-term actions
that might be taken by EPA and other management
agencies to decrease Nr entering the environment from
various sources. Section 6.4 contains specific findings
and recommendations corresponding to each of the
Committee's four study objectives.
   The first objective of the study was to identify and
analyze, from a scientific perspective, the problems Nr
presents in the environment and the links among them.
To accomplish this objective, the Committee examined
the flows of Nr within the food, fiber, feed and bioenergy
production systems and developed lands in the U.S.,
paying special attention to the locations within each
of these systems where Nr is lost to the environment.
The same process was employed for fossil fuel energy
production but, since  all the Nr formed and released
during energy production is lost to the environment, the
Committee identified the important energy producing
sectors that contribute to Nr emissions. The  Committee
found that agriculture and domestic use of fertilizers
to produce food, feed, and fiber (including bioenergy
and BNF) and combustion of fossil fuels are the largest
sources of Nr released into the environment in the U.S.
   The Committee also examined the fate of the Nr
lost to the environment, estimated the amount stored in
different systems (e.g., forest soils) and tracked Nr as
it is transferred from one environmental system (e.g.,
the atmosphere) to another (e.g., terrestrial and aquatic
ecosystems). Source and fate analyses set the stage for
identifying the environmental and human health problems
Nr presents, and the links among them. Using the nitrogen
cascade, the Committee identified the impacts  Nr has
on people and ecosystem functions as it moves through
each system and contributes to adverse public health and
environmental effects, including photochemical smog,
nitrogen-containing trace gases and aerosols, decreased
atmospheric visibility, acidification of terrestrial and
aquatic ecosystems, eutrophication of coastal waters (i.e.,
harmful algal blooms, hypoxia), drinking water concerns,
freshwater Nr imbalances, GHG emissions and subsequent
climate change, and stratospheric ozone depletion.
   The second objective of the study was to evaluate
the contribution an integrated N management strategy
could make to environmental protection. To accomplish
this objective the Committee identified actions that
could be taken to better manage Nr. These actions take
into account the contributions of all Nr sources and
chemical species that adversely impact human health
and environmental systems, and the need to ensure that
solving one problem related to Nr does not exacerbate
another problem or diminish ecosystem services that
support societal demands.
   The third objective of the study was to identify
additional risk management options for EPA's
considerationJn addressing this objective, the Committee
identified four major goals for management actions that
collectively have the potential to decrease Nr losses to
the environment by about  25%. Decreasing Nr emissions
by these actions will result in further decreases in
Nr-related impacts throughout the nitrogen cascade.
The Committee has suggested a number of ways to
attain these management goals, including conservation
measures, additional regulatory steps, voluntary actions,
application of modern technologies, and end-of-pipe
approaches. The Committee notes that these are initial but
significant actions; however, others should be taken once
the recommended actions  are completed and assessed,
and further opportunities are explored in an adaptive
management approach.
   The fourth objective of the study was to make
recommendations to EPA concerning improvements in
Nr research to support risk reduction. The Committee has
provided numerous recommendations for additional Nr
research to support risk reduction activities.

6.1.  Need for Comprehensive Monitoring
ofNr
   In previous sections of this report the Committee
has discussed the importance of monitoring reactive
nitrogen in the environment. The Committee recommends
establishing a program for comprehensive monitoring
of the multiple forms of reactive nitrogen as both
stocks and flows as they pass through different media
and ecosystems. There are two major reasons for this
overarching recommendation. The first purpose of
monitoring is to provide the observational data on trends
that will inform research into the complexity of the

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nitrogen cascade to better identify the most effective
intervention points to reduce damage to human health and
the environment by reactive nitrogen. The second need
for monitoring is to be able to assess the effectiveness
of policy interventions overtime, and to apply the
principles of adaptive management. As it becomes clear
which strategies and policy instruments are effectively
reducing the amounts of Nr entering the environment,
and which are ineffective, it will be necessary to modify
those interventions in response to the monitoring data. As
conditions change (e.g., shifts in the technology of electric
power production, new fuels for transportation, changing
land use patterns and climate change), the nitrogen
cascade will be modified, and the relative importance
of sector specific policies will change. Only through
comprehensive monitoring will it be possible to manage
Nr effectively.
   Finding 20: The Committee has determined that an
integrated approach to monitoring that includes multiple
media (air, land, and water) components and considers
a suite of environmental and human concerns related to
reactive nitrogen in the environment (e.g., Nr effects,
climate  change, human health) is needed. Some of the
phenomena presented in this report need more definition
and verification but, more importantly, as controls are
brought to bear on Nr, improvements need to be measured
to verify and validate successful management strategies.
If the desired improvements are not realized as shown by
the collected data, corrective measures will be required.
Such an adaptive approach acknowledges the likelihood
that management programs will be altered as scientific
and management understanding improve.
   Recommendation 20: The Committee recommends
that EPA initiate discussions and take action to develop a
national, multi-media monitoring program that monitors
sources, transport and transition, effects using indicators
where possible, and sinks ofNr in keeping with the
nitrogen cascade concept. This comprehensive program
should build upon existing EPA and state initiatives as
well as monitoring networks already underway in other
federal agencies such as the  U.S. Geological Survey
programs and the NADP effort.

6.2. Overarching Recommendations
   Human activities have significantly increased the
introduction of Nr into the U.S. environment and, through
radical alterations of land use, have eliminated many
of the natural features that once may have provided
pollutant treatment. While there have been significant
benefits resulting from food production, there have also
been, and continue to be, major risks to the health of both
ecosystems and people due to the introduction of Nr into
the nitrogen cascade.
   In its 1990 report, Reducing Risk, the Science Advisory
Board recommended that the EPA increase its efforts
to integrate environmental considerations into broader
aspects of public policy in as fundamental a manner as
are economic concerns. Other federal agencies often
affect the quality of the environment, e.g., through
the implementation of tax, energy, agricultural, and
international policy, and EPA should work to ensure
that environmental considerations are integrated, where
appropriate, into the policy deliberations of such agencies.
In the current era of increasing responsibilities without
commensurate budgets, intergovernmental cooperation,
partnerships and voluntary programs have become vital
tools for agencies needing to stretch their resources to
fulfill their missions.
   Optimizing the benefits of Nr, and minimizing its
impacts, will require an integrated nitrogen management
strategy that not only involves EPA, but also
coordination with other federal agencies, the States, the
private sector, universities, and a strong public outreach
program. The Committee understands that there are real
economic costs to the recommendations contained in this
report. For each recommendation there  will of necessity
be tradeoffs derived from the varying cost-effectiveness
of different strategies.
The Committee makes four overarching
recommendations:
Overarching Recommendation 1
   The Committee recommends an integrated approach
to the management ofNr.  This approach will likely use
a combination of implementation mechanisms. Each
mechanism must be appropriate to the nature of the
problem  at hand, be supported by critical research on
decreasing the risks of excess Nr, and reflect an integrated
policy that recognizes the complexities and tradeoffs
associated with the nitrogen cascade. Management efforts
at one point in the cascade may be more efficient and cost
effective  than control or intervention at another point.
This is why understanding the nature and dynamics of the
N cascade is critically important.
Overarching Recommendation 2
   The framing of the reactive nitrogen cascade provides
a means for tracking nitrogen as it changes form and
passes through multiple ecosystems and media. This
complexity requires the use of innovative management
systems and regulatory structures to address the
environmental and human health implications of the
massive amounts of damaging forms ofNr. It is difficult
to create fully effective regulations de novofor such a
complex  system so we recommend utilizing adaptive
management to continuously improve the effectiveness
and lower the cost of implementation policies. This in
turn will require a monitoring system that will provide
feedback on the effectiveness of specific  actions taken to
lower fluxes and concentrations ofNr.
Overarching Recommendation 3
   EPA should form an intra-Agency Nr management
task force that will build on existing Nr research and

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management capabilities within the Agency. This
intra-Agency task force should be aimed at increasing
scientific understanding of: (1) Nr impacts on terrestrial
and aquatic ecosystems, human health, and climate, (2)
Nr-relevant monitoring requirements, and (3) the most
efficient and cost-effective means by which to decrease
various adverse impacts ofNr loads as they cascade
through the environment.
Overarching Recommendation 4
   Successful Nr management will require changes in
the way EPA interacts with other agencies. To coordinate
federal programs that address Nr concerns and help
ensure clear responsibilities for monitoring, modeling,
researching and managing Nr in the environment, the
Committee recommends that EPA convene an Inter-
agency Nr management task force. It is recommended
that the members of this inter-agency task force include at
least the following federal agencies: U.S. Department of
Agriculture, U.S. Department of Energy, U.S. Department
of Housing and Urban Development, U.S. Department
of Transportation, National Oceanic and Atmospheric
Administration, U.S. Geological Survey, U.S.  Forest
Service, and Federal Emergency Management Agency.
This task force should coordinate federal programs that
address Nr concerns and help ensure clear responsibilities
for monitoring, modeling, researching, and managing Nr
in the environment. The EPA Office of International and
Tribal Affairs should work closely with the Department of
State to ensure that EPA is aware of international efforts
to control Nr and is developing national strategies that
are compatible with international initiatives.
   Similar recommendations  for coordination and joint
action among and between agencies at both state and
federal levels have been made in the National Research
Council's recent  reports on the Mississippi Basin
(NRC, 2008b, 2009). These intra and inter-agency Nr-
management task forces should take a systems approach
to research, monitoring, and evaluation to inform public
policy related to Nr management. The Committee
proposes that the intra and inter-agency task forces
coordinate the  following activities to implement a systems
approach to Nr management.

Development of methods
   Implementation of a systems approach will require
development of methods to facilitate various aspects of Nr
management. These include methods for: (1)  establishing
and evaluating proposed Nr budgets; (2) using life cycle
accounting approaches for Nr management; (3) gathering
and using data on N fertilizer use and other Nr sources
and fluxes as the basis for informed policies, regulations
and incentive frameworks for addressing excess Nr loads;
(4) evaluating the critical loads approach to air and water
quality management; (5) identifying and using indicators
of excess Nr's  economic damage and effects on human
health and the environment; and (6) using systems-based
approaches for controlling Nr releases to the environment.
Implementing best management practices
(BMPs)
   It will be necessary to improve the scientific
understanding of BMPs that can be used for specific
applications to manage Nr. In particular, this includes
better scientific understanding of:  (1) Nr requirements
in agriculture to ensure adequate food, feed, fiber, and
bioenergy feedstock supply while  also avoiding negative
impacts on the environment and human health; (2) Nr
requirements for urban landscapes (e.g., residential
and commercial) and their maintenance while avoiding
negative impacts on the environment and human health;
(3) planning and pollution prevention, including low
impact development and natural ecosystem service
preservation; (4) use of natural land features and
attributes, such as wetland preservation and enhancement,
natural soil profiles, and buffer strips; and (5) improved
removal of Nr from sewage waste steams at both large-
scale wastewater treatment facilities and individual
subsurface (septic)  systems. In addition, proactive
extension and technology transfer approaches will need to
be established to facilitate adoption of BMPs.
Developing appropriate tools and metrics for
assessing impact from adoption of best manage-
ment practices
   Assessment activities will also  be an important element
of the systems approach to managing Nr. These activities
should include: (1)  quantifying the effectiveness and
impact of policies and regulations focused on reduction
of negative environmental impacts from Nr; (2) assessing
combined carbon (C)  and Nr effects on terrestrial and
aquatic ecosystems; (3) assessing  indicators/endpoints,
costs, benefits, and  risks associated with impairment of
human health and decline and restoration of ecosystem
services; (4) reviewing existing and proposed legislation
for purposes of better integrating or designing  regulatory
activities that recognize the nitrogen cascade or
streamlining procedures for enacting Nr risk reduction
strategies; and (5) evaluating economic  incentives,
particularly those that integrate air, aquatic, and land
sources of excess Nr.
Education, outreach, and communication
   It will be necessary to develop  new education,
outreach, and communication initiatives. As discussed in
this report, this includes a range of targeted outreach and
education programs to manage Nr and achieve desired
environmental outcomes.

6.3. Near-term Management  Goals
   The Committee puts forward four goals for actions
that could be taken by EPA or other management agencies
to decrease Nr entering the environment  from various
sources. We believe these goals can be attained over the
near term (approximately 10-20 years) using existing and
emerging technologies and practices. These suggestions, if
implemented,  have the potential to  reduce total Nr loadings

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to the environment in the U.S. by approximately 25%
below current levels. The Committee believes that these
represent realistic and attainable near-term goals, however
further reductions are undoubtedly needed for many
N-sensitive ecosystems and to ensure that health-related
standards are maintained. The Committee understands that
actual policy decisions on the implementation of programs
to limit Nr releases to the environment may differ from
those listed below for a variety of reasons, but believes that
an aggressive level of action, as represented by these goals,
is critical given the growing demand for food and fiber
production and energy use from population pressures and
economic growth. The rationale for these goals is set forth
below, along with recommended management options for
achieving the goals.

Management Goal 1. Controls on NOX emissions
from mobile and stationary sources
   The Clean Air Act (1970) and its Amendments (1990)
have resulted in NOX emissions that are less than 50
percent of what they would have been without existing
controls.  While this is an admirable accomplishment,
there is still a need to seek  improvements. NOX emissions
are an order of magnitude greater than at the beginning
of the twentieth century. As a consequence, there
remain significant negative impacts on both humans
and ecosystems. In 2002, coal-fired utilities generated
approximately 1.3 Tg N annually (see Figure 3). If all
coal-fired plants used state-of-the-art NOX controls, this
number could be reduced by 0.6 Tg N/yr (calculations
performed by Cohen, 2008); in fact, 2008 emissions have
been reduced by 0.3 Tg N/yr from 2002 levels (see Figure
3), so in essence, half the reduction has already been
accomplished. The EPA should continue to reduce NOX
emissions from major point sources, including electric
generating stations and industrial sources, expanding
the use of market mechanisms such as cap and trade.
Under this scenario, it is likely that high-efficiency, low-
emission power plants will be built for energy needs.
Some controls on NOX emissions are implemented only
in the ozone season (May to September) (U.S. EPA
2009c). To protect welfare  and avoid adverse effects
on ecosystems, NOX emissions controls should be
implemented year round.
   For mobile sources, emissions for highway and
off-highway sources are approximately 2.2 Tg N/yr
and 1.2 Tg N/yr, respectively. EPA is in the process of
implementing a number of  regulations that will reduce
NOX from mobile sources (see Appendix F). The
implications of these recent regulations are not reflected
in the quantitative analysis  presented in this report. EPA
projects future year decreases in emissions (see Figure
5 in Section 2.2.1). However, better controls are needed
for on-road heavy duty diesel vehicles and off-road
vehicles,  which include locomotives, construction, farm,
landscaping equipment, and marine vehicles. For these
off-road vehicles, 80-90% NOX removal is technically
achievable (deNevers, 1995; Koebel et al., 2004).
Assuming a 40% reduction for these sources, there is a
potential reduction of 1.4 Tg. The total reduction for both
mobile and stationary sources is then approximately 2
Tg N/yr. Part of achieving such levels  of compliance will
require the implementation of inspection and maintenance
programs or road-side monitoring.
   The Committee cautions, however, that achieving
such a goal may be inadequate for many areas to meet
the new 60 to 70 ppb ozone standard recommended
by the EPA Clean Air Scientific Advisory Committee
(CASAC) (U.S. EPA CASAC, 2008) or even the 75 ppb
standard currently promulgated. Additional measures
such as increasing the role of solar- and wind-generated
electricity, wider use of hybrid and electric cars, and
public transit conducive to energy conservation and
reduced emissions should be  promoted.
   Management Goal 1. The Committee estimates
   that if EPA were to expand its NOX  control efforts
   for emissions of mobile sources and power plants
   and include implementation of year round controls
   on stationary sources to protect welfare and avoid
   adverse effects on ecosystems, a 2.0 Tg N/yr decrease
   in the generation of reactive nitrogen could be
   achieved. It is believed that coal-fired utilities could
   experience a reduction of 0.6 Tg N/yr. Since 2002,
   emissions have already been reduced by at least 0.3
   Tg N/yr; hence, this represents an additional 0.3 Tg
   N/yr. Approximately 3.4 Tg N/yr can be attributed to
   mobile sources (highway,  off-highway). Assuming a
   conservative 40% reduction, an additional 1.4 Tg N/yr
   could be reduced.

Management Goal 2. Nr discharges and emis-
sions from agricultural lands and landscapes
   Section 5.3.4 of this report reviews  the various
methods that can be used to improve Nr management in
agricultural systems. The Committee estimates that crop
N-uptake efficiencies can be increased by up to 25% over
current levels through a combination of knowledge-based
practices and advances in fertilizer technology (such as
controlled release  and inhibition of nitrification). Crop
output can be increased while reducing total Nr by up
to 20% of applied synthetic fertilizers, approximately
2.4 Tg N/yr below current levels of Nr additions to the
environment. These are appropriate management goals
with today's available technologies. Further progress is
possible through expanded research programs.
   The Committee is concerned about current policies
and practices governing biofuel development. Acreage
devoted to corn production has increased substantially
for corn based ethanol production during the past several
years (with nearly one-third of the crop currently devoted
to bioethanol production), with fertilizer nitrogen use on
corn increasing by at least 10% (an additional 0.5 Tg N/
yr), largely to meet biofuel feedstock crop demand. In the

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absence of Nr controls and a failure to implement best
practices, current biofuels policies will make it extremely
difficult to reduce Nr transfers to soils, water and air
(Simpson et al., 2008). Integrated management strategies
will be required.
   The Committee also notes with concern the increase
of N2O in the atmosphere. The Committee believes that
GHG emissions trading will provide both opportunities
and challenges for mitigating Nr environmental and health
impacts. Policies and regulations should consider how to
reward reductions of N-related GHG. Biofuel subsidies that
accurately account for Nr contributions to GHG emissions,
certification of individual biofuel plants for GHG impact,
and rewards for farmers who reduce N2O emissions
are examples of how an integrated strategy can reduce
agricultural GHG impacts. For additional production of
liquid biofuels beyond the grandfathered amount in the
Energy Independence and Security Act (EISA), EPA has
the power to exercise some controls on N2O emissions
through the life cycle GHG accounting requirements. It is
the opinion of the Committee that Section 204 of the EISA
calling on the Agency to  adopt a life cycle approach to the
assessment of future renewable fuel standards is a positive
step toward a comprehensive analysis.
   Section 5.3.4 of this report reviews methods of
controlling Nr from landscape runoff through the use of
natural or restored wetlands, urban areas, and through
the use of best management practices. The Committee
finds that flows of Nr into streams, rivers, and coastal
systems can be reduced by approximately 20% (~1 Tg N/
yr) through improved  methods of landscape management
and without undue disruption to human commercial and
aesthetic activities.
   Management Goal 2. The Committee estimates that
   crop N-uptake efficiencies can be increased by up to
   25% over current practices through a combination of
   knowledge-based practices and advances in fertilizer
   technology amounting to -2.4 Tg N/yr below current
   amounts ofNr additions to the environment. The
   Committee further estimates that excess flows of
   Nr into streams, rivers, and coastal systems can be
   decreased by approximately 20% (~1 Tg N/yr) through
   improved landscape management and without undue
   disruption to agricultural production.

Management Goal 3. Ammonia emissions from
livestock management and manure handling
   In spite of gains made over the last several  decades in
decreasing the amount of NOX emitted from stationary
and mobile combustion sources, the total amount of Nr
released into the atmosphere has remained relatively
constant. This is related to the essentially unregulated
release of ammonia from livestock operations.
As discussed in Section 2.2.3, at the present time,
fewer livestock are required to produce more animal
products than in the past. For example, since 1975 milk
production has increased linearly at the rate of ~ 180
kg milk per cow /yr while milk cow herd population
decreased at the rate of-69,000 head peryr (i.e., the
60% greater amount of milk produced in 2006 compared
to 1970 required 25% fewer cows). Animal inventories
declined by 10% for beef brood cows from 36 million
head in 1970 to 33 million head in 2006, and the
inventory of breeder pigs and market hogs declined
8% from 673 million head to 625 million head in the
same period, even with similar or greater annual meat
production. These trends resulted from greater growth
rates of animals producing more meat in a shorter
amount of time. In 1970, broilers were slaughtered after
80 days on feed at 1.7 kg live weight, but by 2006 the
average weight was 2.5 kg after only 44 days on feed.
These trends in requiring fewer animals to produce
more animal food products through improved diet and
increased production efficiency will continue.
   Implementation of improved methods of livestock
management and manure handling and treatment to
decrease NH3 emissions that have been developed
since 1990 will further decrease ammonia and other
gases and odor emissions. For example, sawdust litter
helps decrease NH3 emissions from pig manure with
44-74% of manure N converted to N2. Storage covers
for slurry storage tanks, anaerobic lagoons, and earthen
slurry pits decrease emissions from those containments.
Anaerobic digestion in closed containment has been
studied for many types of applications. Recent research
demonstrates reduction in NH3 emissions after a
permeable cover was installed (e.g., a polyethylene cover
decreased NH3 emissions by -80%). A well managed
swine lagoon can denitrify approximately 50% of the
excreted N to N2. Recently engineered developments
utilizing closed loop systems (Aneja et al., 2008a)
substantially reduce atmospheric emissions of ammonia
(> 95%) and odor at hog facilities. Based upon recently
demonstrated reduction of NH3 emissions from swine
and poultry production, a moderate reduction of 50%
from 1990 NH3 emission estimates for swine and poultry
production should be attainable (Table 17). Because
of the larger land area involved in dairy and beef
production and the lack of effort that has been exerted in
mitigating NH3 emissions, a more modest and reachable
goal of decreasing NH3 emissions by 10% through
improvements in animal diet and manure management is
proposed (Table 17).
   Management Goal 3. The Committee estimates that
   livestock-derived NH^ emissions can be decreased by
   30% (a decrease of 0.5 Tg N/yr) by a combination of
   BMPs and engineered solutions. This is expected to
   decrease PM2.s  by -0.3 jj.g/m3 (2.5%), and improve
   health of ecosystems by achieving progress towards
   critical load recommendations. Additionally we
   estimate that NH^ emissions derived from fertilizer
   applications can be decreased by 20% (a decrease

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      Table 17: Estimates for potential decreases in NH3 emissions from livestock manure in the
                                              United States
NH3 Source
Dairy
Beef
Poultry
Swine
Goat/sheep
Horse
Total

23.1
27.1
27.5
17.5
1.6
2.9
100
^^^
0.37
0.44
0.44
0.28
0.03
0.05
1.61
Estimated D
10
10
50
50
10
10

ecrease of NHa
Tg N/yr
0.040
0.040
0.220
0.140
0.003
0.005
0.45
Estimate is based on livestock emissions of 1.6 Tg from Table 1.
   of-0.2 Tg N/yr), through BMPs that focus on
   improvements related to application rate, timing,
   and placement.

Management Goal 4. Discharge of Nr from
developed lands and point sources
   National loadings of Nr to the environment from
public and private wastewater point sources are relatively
modest in comparison with global Nr releases; however,
they can be important local sources with associated
impacts, especially in highly-populated watersheds. The
Committee has estimated that sewage containing Nr from
human waste contributes 1.3 Tg N/yr to the terrestrial
inputs of nitrogen (Table 1).
   The Committee has also estimated that turf fertilizer
usage contributes 1.1 Tg N/yr to terrestrial inputs, a load
that could potentially be cut by about one third (Section
2.2.4). The Committee did not provide estimates for
general stormwater and nonpoint source runoff nitrogen
load reductions specific to developed or urban areas
- runoff concentrations and loads are highly variable
reflecting geographic and climatic conditions throughout
the U.S. and equally variable removal efficiencies from
standard treatment BMPs. This is shown in a summary
of the International Stormwater Best Management
Practices Database (Geosyntec Consultants, Wright
Water Engineers, Inc., 2008). However, most BMPs are
effective because they provide the beneficial biochemical
conditions of wetlands, the biophysical controls described
in Section 5.3.4 and Appendix C. These benefits, and the
application of BMPs, are recommended in overarching
recommendation 4, as well as in the preceding
Management Goal 2 as applied to agricultural lands.
Similar stormwater and nonpoint source management
benefits specific to developed lands should be anticipated
with BMP application in those areas.
   Denitrification processes as applied to human
waste at sewage treatment plants are well-studied and
growing in application. Performance of these engineered
solutions, collectively referenced as biological nitrogen
removal (BNR), can be more rigorously controlled
than for stormwater and nonpoint source BMPs. Recent
publications by the U.S EPA (2007f, 2008e,f) have
summarized the state of and the capability for nitrogen
removal, and have reported that technologies to achieve
effluent concentrations of 3 mg total nitrogen per liter
(TN/L) or less are readily available. However, plant
capacity and design, wastewater characteristics, and
climate conditions can all affect the ability of a facility
to remove nitrogen. EPA's review of 2003-2006 data
for 16 facilities that remove nitrogen to varying degrees
found a range of final effluent TN concentrations of 1.0
to 9.7 mg/L, with an average  of 5.6 mg/L. In general,
very small facilities (<0.1 MOD) do not perform as
well, with a final TN concentration ranging from 6-12
mg/L. Treatment performance varied and exceeded 5 mg
TN/L at some of the facilities. Given these conditions,
and performance uncertainties, it seems reasonable to
conclude that removal efficiencies  in the range of 40-
60% below standard effluent nitrogen loads could be
readily attained. Based on the human waste load of 1.3
Tg N/yr, this would yield a decrease in total nitrogen
load of between 0.5 and 0.8 Tg/yr.  Using data provided
by Maryland Department of the Environment (2006) and
the Connecticut Department of Environmental Protection
(2007), two states that have promoted nitrogen removal
technologies as solutions to coastal eutrophication, EPA
(2007f) has constructed cost estimates of upgrading the
performance of sewage treatment plants ranging from
$990,000 to $1.74 million per MOD treated.
   There are two funding sources of significance
authorized in the CWA that are used to fund projects

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relevant to the control of Nr. Section 319 establishes
state nonpoint source management programs to plan for
and implement management measures that abate sources
of nonpoint pollution from eight source categories,
including both urban and agricultural sources; however,
the CWA disallows use of 319 funds for NPDES
permit requirements, so urban areas with stormwater
permits do not qualify for Section 319 funding. Over
the years, section 319 has made available, through 60%
matching funds, over $1.6 billion in assistance. The
much larger source of funding comes under Title VI of
the CWA, which has provided over $24 billion (federal)
for the construction of treatment facilities for point
sources of wastewater over the past 20 years, although
only a fraction of this amount has been dedicated to
denitrification processes. Title VI "state revolving" loan
funds can be used for stormwater management, as well as
other water pollution management activities, but not all
states have chosen to use funds beyond traditional sewage
treatment plant infrastructure needs because of the large
backlog of demand for those purposes.
  In 2009, under the American Reinvestment and
Recovery Act (ARRA), the CWA Clean Water  State
Revolving Fund (CWSRF) received a $4 billion boost
for clean water infrastructure and the CWSRF for fiscal
year 2010 was tripled over the prior years to two billion
dollars. These additional funds not only provide for jobs
creation, as intended by Congress, but provide  states with
resources to reduce the backlog of clean water  projects,
which also often include nutrient management  needs.
The ARRA funds also emphasized the use of CWSRF
dollars for stormwater and nonpoint source management
and energy  savings under a "green infrastructure"
requirement. A 20%  set-aside for green infrastructure
was a requirement of AARA CWSRF funding and was
used widely for projects that included reductions in GHG
emissions, land-based low impact development BMPs
to reduce runoff and improve runoff quality, and other
innovative practices  to  treat wastewater and runoff. A
green infrastructure requirement is being continued in the
fiscal year 2010 CWSRF allocation.
   Management Goal 4. The Committee recommends
   that a high priority be assigned to increasing funding
  for nutrient management. We estimate that a decrease
   in Nr emissions from human sewage of between 0.5
   and 0.8 TgN/yr can be achieved, with additional
   decreases likely with increased stormwater  and
   nonpoint source BMP application support.

6.4. Summary of Specific Findings and
Recommendations Corresponding to the
Four Study Objectives
  In this report the Committee  has provided specific
findings and recommendations to assist EPA in its
understanding and management of nitrogen-related air,
water, and soil pollution issues.  The specific findings and
recommendations corresponding to each of the four study
objectives are summarized below.

Study Objective #1: Identify and analyze, from a
scientific perspective, the problems Nr presents
in the environment and the links among them.
   In general, the Committee finds that uncertainty
associated with rapid expansion of biofuels, losses of Nr
from grasslands, forests, and urban areas, and the rate and
extent of denitrification have created the need to measure,
model, and report all forms of Nr consistently and
accurately. Addressing this need will decrease uncertainty
in the understanding of the fate of Nr that is introduced
into the environment and lead to a better understanding
of the impacts of excess Nr on the health of people and
ecosystems. This should be accomplished through a
coordinated effort among cognizant federal and state
agencies and universities.
   The Committee recommends that EPA routinely
and consistently account for the presence of Nr in the
environment in forms appropriate to the medium in which
they occur (air, land, and water) and that accounting
documents be produced and published periodically.
Specific Findings:
  Rapid expansion of corn-ethanol production has the
  potential to increase N fertilizer use through expanding
  corn production and its associated N fertilizer inputs.
  Development of cellulosic ethanol industry will require
  cultivation for cellulosic crops, which will also require
  N fertilizer. Distillers grains are changing animal
  diets and affecting N recycling in livestock. Both
  have important consequences for the effective future
  management of Nr. (Finding #4 - also pertains to study
  objectives 2 and 4)
  Although total  N budgets within  all terrestrial systems
  are highly uncertain, Nr losses from grasslands and
  forests (vegetated) and urban (populated) portions
  of the N cascade appear to be higher,  on a percent of
  input basis, than from agricultural lands. The relative
  amount of these losses ascribed to leaching, runoff,
  and denitrification are as uncertain as the N budgets
  themselves. (Finding #9)
  Denitrification of Nr in terrestrial and aquatic systems
  is one of the most uncertain parts of the nitrogen cycle.
  Denitrification is generally considered to be a dominant
  N loss pathway in both terrestrial and aquatic systems,
  but it is poorly quantified.  (Finding #10 - also pertains
  to study objective  4)
  The Committee finds that there is a need to measure,
  compute, and report the total amount of Nr present in
  impacted systems  in appropriate  units. Because what is
  measured influences what we are able to perceive and
  respond to, in the case of Nr, it is especially critical to
  measure total amounts and different chemical forms at
  regular intervals over time. (Finding #13 - also pertains
  to study objective  4)

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Specific Recommendations:
  The Committee recommends that EPA routinely and
  consistently account for the presence of Nr in the
  environment in forms appropriate to the medium
  in which they occur (air, land, and water) and that
  accounting documents be produced and published
  periodically (for example, in a fashion similar to
  National Atmospheric Deposition Program summary
  reports). The Committee understands that such an
  undertaking will require substantial resources, and
  encourages the Agency to develop and strengthen
  partnerships with appropriate federal and state agencies
  and private sector organizations with parallel interests
  in advancing the necessary underlying science of Nr
  creation, transport and transformation, impacts, and
  management. (Recommendation #13 - also pertains to
  study objective 4)
  EPA should work with USDA and universities
  to improve understanding and prediction of how
  expansion of biofuel production, as mandated by the
  2007 EISA, will affect Nr inputs and outputs from
  agriculture and livestock systems.  Rapid expansion
  of biofuel production has the potential to increase N
  fertilizer use through expansion of corn production area
  and associated N fertilizer inputs, and from extending
  cultivation of cellulosic materials that will also need
  N inputs. Current models and understanding are not
  adequate to guide policy on how to minimize impact of
  biofuel expansion on environmental concerns related to
  Nr. (Recommendation #4)
  EPA should join with USDA, DOE, and universities
  in efforts to ensure that the N budgets of terrestrial
  systems are properly quantified and that the magnitudes
  of at least the major loss vectors are known.
  (Recommendation #9 - also pertains to study objectives
  2 and 4)
  EPA, USDA, DOE, and universities should work
  together to ensure that denitrification in soils and
  aquatic systems is properly quantified, by funding
  appropriate research. (Recommendation #10 - also
  pertains to study objective 4)

Study Objective #2: Evaluate the contribution an
integrated N management strategy could make
to environmental protection.
   In general, the Committee finds that effective
management of Nr in the environment must recognize
the existence  of tradeoffs across a number of impact
categories involving the cycling of nitrogen and other
elements. In addition, an integrated multi-media approach
to monitoring Nr is needed.
The Committee recommends that EPA:
1. Develop a uniform assessment and management
  framework that considers the effects of Nr loading
  over a range of scales reflecting ecosystem, watershed,
  and regional levels. The framework should include
  all inputs related to atmospheric and riverine delivery
  of Nr to estuaries, their comprehensive effects on
  marine eutrophication dynamics and their potential for
  management
2. Examine the full range of traditional and ecosystem
  response categories, including economic and
  ecosystem services, as a basis for expressing Nr
  impacts in the environment, and for building better
  understanding and support for integrated management
  efforts.
Specific Findings:
  There has been a growing recognition of eutrophication
  as a serious problem in aquatic systems (NRC, 2000).
  The last comprehensive National Coastal Condition
  Report was published in 2004 (EPA, 2004) and
  included an overall rating of "fair" for estuaries,
  including the Great Lakes, based on evaluation of
  over 2000 sites. The water quality index, which
  incorporates nutrient effects primarily as chlorophyll-a
  and dissolved oxygen impacts, was also rated "fair"
  nationally. Forty percent of the sites were rated "good"
  for overall water quality, while 11% were "poor" and
  49% "fair." (Finding #11)
  The Committee finds that reliance on only one
  approach for categorizing the measurement of Nr is
  unlikely to result in the desired outcome of translating
  N-induced degradation into the level of understanding
  needed to develop support for implementing effective
  Nr management strategies. (Finding #14)
  Effective management of Nr in the environment must
  recognize the existence of tradeoffs across impact
  categories involving the cycling of other elements,
  particularly carbon and phosphorus. (Finding #18)
  The Committee has determined that an integrated
  approach to monitoring that includes multiple media
  (air, land, and water) components and considers a
  suite of environmental and human concerns related
  to reactive nitrogen in the environment (e.g., Nr
  effects, climate change, human health) is needed.
  Some of the phenomena that presented in this
  report need more definition and verification. More
  importantly, however, as controls are brought to bear
  on Nr, improvements need to be measured to verify
  and validate successful management strategies. If
  the desired improvements are not realized as shown
  by the collected data, corrective measures will be
  required. Such an adaptive approach acknowledges
  the likelihood that management programs will be
  altered as scientific and management understanding
  improve. (Finding #20 - also pertains to study
  objective 3)
Specific Recommendations:
  The Committee recommends that EPA develop a
  uniform assessment and management framework that
  considers the effects of Nr loading over a range of

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scales reflecting ecosystem, watershed, and regional
levels. The framework should include all inputs related
to atmospheric and riverine delivery of Nr to estuaries,
their comprehensive effects on marine eutrophication
dynamics, and their potential for management.
(Recommendation #11)
It is recommended that the EPA examine the full range
of traditional and ecosystem response categories,
including economic and ecosystem services, as a basis
for expressing Nr impacts in the environment, and for
building better understanding and support for integrated
management efforts. (Recommendation #14)
The Committee recommends that the integrated
strategies for Nr management outlined in this report be
developed in cognizance of the tradeoffs associated with
reactive nitrogen in the environment, consistent with the
systems approach of overarching recommendations 2
and 3. Specific actions should include:
 • Establishing a framework for the integrated
  management of carbon and reactive nitrogen
 • Implementing a  research program that addresses the
  impacts of tradeoffs associated with management
  strategies for carbon, reactive nitrogen, and other
  contaminants of concern
 • Implementing a  research and monitoring program
  aimed at developing an understanding of the
  combined impacts of different nitrogen management
  strategies on the interchange of reactive nitrogen
  across environmental media. (Recommendation #18)
In cooperation with the Department of Agriculture,
U.S. Army Corps of Engineers, U.S. Fish and
Wildlife Service, and the and the Federal Emergency
Management Agency, the EPA should develop
programs to encourage wetland restoration and
creation, with strategic placement of these wetlands
where reactive nitrogen is highest in ditches, streams,
and rivers. The Agency should also address the
means of financing, governance, monitoring, and
verification. Such programs might be modeled on the
Conservation Reserve Program or extant water quality
and environmental trading programs, but need not be
limited to  current practices. (Recommendation #15e -
also pertains to study objective 3)
The Committee recommends that EPA initiate
discussions and take action to develop a national,
multi-media monitoring program that monitors sources,
transport and transition, effects using indicators where
possible, and sinks of Nr in keeping with the nitrogen
cascade concept. This comprehensive program should
build upon existing EPA and state initiatives, as well as
monitoring networks already  underway in other federal
agencies such as the U.S. Geological Survey programs
and the NADP effort. (Recommendation #20 - also
pertains to study objective 3)
Study Objective #3: Identify additional risk
management options for EPA's consideration.
   In general, the Committee finds that a number of risk
management actions should be considered to reduce Nr
loading and transfer to the environment. The Committee
recommends risk management actions that include farm-
level improvements in manure management, actions to
reduce atmospheric emissions of Nr, and interventions to
control Nr in water management programs.
Specific Findings:
  Farm-level improvements in manure management can
  substantially reduce Nr load and transfer.  There are
  currently very few incentives or regulations to decrease
  these transfer and loads, despite the existence of
  management options to mitigate. (Finding #6)
  Scientific uncertainty about the origins, transport,
  chemistry, sinks, and export of Nr remains high, but
  evidence is strong that atmospheric deposition of Nr
  to the earth's surface, as well as emissions from the
  surface to the atmosphere, contribute substantially to
  environmental and health problems. Nitrogen dioxide,
  NC>2, is often a small component of NOy, the total
  of oxidized nitrogen in the atmosphere. The current
  NAAQS for NO2, as an indicator of the criteria pollutant
  "oxides of nitrogen," is inadequate to protect health
  and welfare. NOy should be considered seriously as a
  supplement or replacement for the NO2 standard and in
  monitoring. Atmospheric emissions and concentrations
  of Nr from agricultural practices (primarily in the form
  of NH3) have not been well monitored, but NH4+ ion
  concentration and wet deposition (as determined by
  NADP and NTN) appear to be increasing, suggesting
  that NH3 emissions are increasing. Both wet and dry
  deposition contribute substantially to NHX removal,
  but only wet deposition is known with much scientific
  certainty. Thus, consideration should be given to adding
  these chemically reduced and organic forms of Nr to the
  list of criteria pollutants. (Finding #8)
  Meeting Nr management goals for estuaries, when a
  balance  should be struck between economic, societal,
  and environmental needs, seems unlikely  under current
  federal law. Enforceable authorities over nonpoint
  source, stormwater,  air (in terms of critical loads),
  and land use are not adequate to support necessary Nr
  controls. Funding programs are presently  inadequate
  to meet existing pollution control needs. Furthermore,
  new technologies and management approaches are
  required to meet ambitious Nr control  needs aimed at
  restoring national water quality. (Finding #12 - also
  pertains to study objective 4)
  Intervention to control Nr under most water
  management programs generally occurs in three ways:
   • Prevention or source controls
   • Physical, chemical or biological "dead  ending" or
    storage within landscape compartments where it

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    is rendered less harmful (e.g., long-term storage
    in soils or vegetation; denitrification, primarily in
    wetlands; reuse)
   • Treatment using engineered systems such as
    wastewater treatment plants or BMPs for stormwater
    and nonpoint source runoff
   While most management programs focus on the
third (treatment) approach, there are opportunities for
combining the three that can be more effective and cost
less. (Finding #15)
  The Committee finds that there have been persistent
  increases in the amounts of Nr that have been emitted
  into and retained within various ecosystems, affecting
  their functioning. Unless this trend is reversed, it
  will become increasingly difficult for many of these
  ecosystems to provide the services upon which human
  well-being depends. The Committee believes that there
  is a need to regulate certain forms of Nr to address
  specific problems related to excess Nr, and we believe
  that the best approach for an overall management
  strategy is the concept of defining acceptable total
  Nr critical loads for a given environmental system.
  (Finding #16  - also pertains to study objective 4)
  Current EPA policy (40 CFR Part 51, Clean Air Fine
  Particle Implementation Rule) discourages states from
  controlling ammonia emissions as part of their plan
  for reducing PM2.5 concentrations. In this rulemaking
  (Federal Register 72(79): 20586-20667), EPA has
  stated that "ammonia reductions may be effective
  and appropriate for reducing PM2 5 concentrations in
  selected locations, but in other locations such reductions
  may lead to minimal reductions in PM2 5 concentrations
  and increased atmospheric acidity." Ammonia is  a
  substantial component of PM2 5 in most polluted areas
  of the U.S. at most times. While it is true that reducing
  NH3 emissions might increase the acidity of aerosols
  and precipitation, the net effect of NH3 on aquatic and
  terrestrial ecosystems is to increase acidity. After being
  deposited onto the earth's surface, NH4+ is, under most
  circumstances, quickly nitrified, increasing the acidity
  of soils and waters. The Committee is unaware of any
  evidence that NH3 reduces the toxicity of atmospheric
  aerosols or that high concentrations of NH3 occur
  naturally over any substantial area of the U.S. It has
  not yet been established which components of PM
  have substantive  effects on human health but the total
  concentration of PM2 5 correlates with morbidity and
  mortality, and NH3 contributes to PM2 5. The visibility,
  degradation, and other adverse effects associated with
  PM2 5 are related to aerosol surface area or mass where
  NH4+ certainly plays a role. (Finding #17)
Specific Recommendations:
  A policy, regulatory, and incentive framework is
  needed and should be developed to improve manure
  management to reduce Nr load and ammonia
transfer, taking into account phosphorus load issues.
(Recommendation #6)
EPA should re-examine the criteria pollutant "oxides of
nitrogen" and the indicator species, NO2, and consider
adding chemically reactive nitrogen as a criteria
pollutant and NHX and NOy as indicators to supplement
the NO2 National Ambient Air Quality Standards.
(Recommendation #8a)
The Committee recommends that monitoring of NHX
and NOy begin as soon as possible to supplement
the existing network of NO2 compliance monitors.
(Recommendation #8b)
The Committee recommends that EPA reevaluate
water quality management approaches, tools, and
authorities to ensure Nr management goals are
attainable, enforceable, and the most cost-effective
available. Monitoring and research programs should
be adapted  as necessary to ensure they are responsive
to problem definition and resolution, particularly
in the development and enhancement of nitrogen
removal technologies and best management practices,
and continue to build our level of understanding
and increase our ability to meet management goals.
(Recommendation #12 - also pertains to study
objective 4)
To better address Nr runoff and discharges from the
peopled landscape, the Committee recommends that
EPA:
 • Evaluate  the suite of regulatory and non-regulatory
  tools used to manage Nr in populated areas from
  nonpoint sources, stormwater and domestic sewage,
  and industrial wastewater treatment facilities,
  including goal-setting through water quality standards
  and criteria; and
 • Determine the most effective regulatory and
  voluntary mechanisms to apply to each source
  type with special attention to the need to regulate
  nonpoint source and related land use practices.
  (Recommendation #15a)
 • Review current regulatory practices for point sources,
  including both wastewater treatment plants and
  stormwater, to determine adequacy and capacity
  towards meeting national Nr management goals; and
 • Consider technology limitations, multiple pollutant
  benefits, and funding mechanisms as well as potential
  impacts on climate change from energy use and
  greenhouse gas emissions, including nitrous oxide.
  (Recommendation #15b)
 • Set Nr management goals on a regional/local basis,
  as appropriate, to ensure most effective use of limited
  management dollars; and
 • Fully consider "green" management practices such as
  low-impact development and conservation measures

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    that preserve or re-establish Nr removing features to
    the landscape as part of an integrated management
    strategy along with traditional engineered best
    management practices. (Recommendation #15c)
  The Committee recommends that the Agency work
  toward adopting the critical-loads approach concept
  in determining thresholds for effects of excess Nr on
  terrestrial and aquatic ecosystems. In carrying out
  this recommendation, the Committee recognizes that
  in many cases it will be necessary for the Agency to
  enter into new types of research, policy, and regulatory
  agreements with other federal, state, and tribal
  units based on cooperative, adaptive, and systemic
  approaches that derive from a common understanding
  of the nitrogen cascade. (Recommendation #16 - also
  pertains to study objective 4)
  The Committee recommends that the EPA presumption
  that NH3 is not a PM2 5 precursor should be reversed
  and states should be encouraged to address NH3 as a
  harmful PM2 5 precursor.  (Recommendation #17)

Study Objective #4: Make recommendations to
EPA concerning improvements in Nr research to
support risk reduction.
   The Committee has recommended research in
key areas to support risk reduction. The Committee's
recommendations include research to advance the
understanding of: the quantity and fate of Nr applied
to major crops; how to accelerate crop yields while
increasing N fertilizer uptake efficiency; agricultural
emissions of forms of Nr; atmospheric deposition of
Nr; and the potential for amplification of Nr-related
climate impacts.
Specific Findings:
  Crop agriculture receives 60% of U.S. annual new
  Nr inputs from anthropogenic sources (9.8 Tg from
  N fertilizer, 7.7 Tg from crop BNF versus 29 Tg of N
  anthropogenically introduced into the U.S. environment
  peryear) and accounts for 58% (7.6 Tg) of total U.S.
  Nr losses from terrestrial systems to air and aquatic
  ecosystems, yet current monitoring of fertilizer use
  statistics by federal agencies is inadequate to accurately
  track trends in quantities and fate of N applied to major
  crops and the geospatial pattern by major watersheds.
  (Finding #1)
  Nr inputs to crop systems are critical to sustain crop
  productivity and soil quality. Moreover, given limited
  land and water resources, global population growth,
  and rapid economic development in the world's most
  populous countries, the challenge is to accelerate
  increases in crop yields on existing farm land while
  also achieving a substantial increase in N fertilizer
  uptake efficiency. This process is called "ecological
  intensification"  because it recognizes the need to
  meet future food, feed, fiber, and energy demand of
  a growing human population while also protecting
  environmental quality and ecosystem services for future
  generations (Cassman, 1999). More diverse cropping
  systems with decreased Nr fertilizer input may also
  provide an option on a large scale if the decrease in
  Nr losses per unit of crop production in these diverse
  systems can be achieved without a decrease in total
  food production, which would trigger indirect land
  use change to replace the lost production and negate
  the benefits. However, crop cultivars and agronomic
  practices are changing rapidly, which changes N
  requirements, but current efforts in research, extension,
  and conservation programs on N management within
  these rapidly evolving systems are not adequate to
  meet the challenge of providing better information to
  increase NFUE. (Finding #2)
  Nitrous oxide  emissions from the Nr inputs to
  cropland from fertilizer, manure, and legume
  fixation represent a  large proportion of agriculture's
  contribution to greenhouse gas emissions, and
  the importance of this source of anthropogenic
  greenhouse gas will likely increase unless NFUE
  is markedly improved in crop production systems.
  Despite its importance, there is considerable
  uncertainty in the estimates of nitrous oxide emissions
  from fertilizer, and research should focus on reducing
  this uncertainty.  (Finding #3)
  There are no nationwide monitoring networks in the
  U.S. to quantify agricultural emissions of greenhouse
  gases, NO, N2O, reduced sulfur compounds, VOCs,
  and NH3. In contrast, there is a large network in place
  to assess the changes in the chemical climate of the
  U.S. associated with fossil fuel energy production, i.e.,
  the National Atmospheric Deposition Program/National
  Trends Network  (NADP/NTN) which has been
  monitoring the wet deposition of sulfate (SO42"), NO3",
  and NH4+ since 1978. (Finding #5)
  Synthetic N fertilizer application to urban gardens
  and lawns amounts to approximately 10% of the total
  annual synthetic  N fertilizer used in the U.S. Even
  though this N represents a substantial portion of total
  N fertilizer use, the efficiency with which it is used
  receives relatively little attention. (Finding #7)
  The biogeochemical cycle of Nr is linked to climate
  in profound but nonlinear ways that are, at present,
  difficult to predict. Nevertheless, the potential for
  significant amplification of Nr-related  impacts is
  substantial, and should be examined in more complete
  detail. (Finding #19)
Specific Recommendations:
  The Committee recommends increasing the specificity
  and regularity of data acquisition for fertilizer
  application to major agricultural crops in terms of
  timing and sufficiently small application scale (as
  well as for urban residential and recreational turf) by
  county (or watershed) to better inform decision-making

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about policies and mitigation options for reducing Nr
load in these systems, and to facilitate monitoring and
evaluation of impact from implemented policies and
mitigation efforts. (Recommendation #1)
To obtain information on Nr inputs and crop
productivity the Committee recommends that:
 • Data on NFUE and N mass balance, based on
  direct measurements from production-scale fields,
  be generated for the major crops to identify which
  cropping systems and regions are of greatest concern
  with regard to mitigation of Nr load,  and to better
  focus research investments, policy development,
  and prioritization of risk mitigation strategies.
  (Recommendation #2a)
 • Efforts at USD A and universities be promoted to:
  (1) investigate means to increase the rate of gain in
  crop yields on existing farm land while increasing
  N fertilizer uptake efficiency, and (2) explore the
  potential for more diverse  cropping systems with
  lower N fertilizer input requirements to the extent
  that large-scale adoption of such systems would not
  cause indirect land use change. (Recommendation
  #2b)
 • EPA work closely with the U.S. Department of
  Agriculture (USD A), Department of Energy (DOE),
  the National Science Foundation (NSF), and
  universities to help identify research and education
  priorities to support more efficient use and better
  mitigation of Nr applied to agricultural systems.
  (Recommendation #2c)
The Committee recommends that EPA ensure that the
uncertainty in estimates of nitrous oxide emissions
from crop agriculture be greatly reduced through the
conduct of EPA research and through coordination of
research efforts more generally with other agencies
such as USD A, DOE, NSF, and with research
conducted at universities.  (Recommendation #3)
The status and trends of gases and paniculate matter
emitted from agricultural emissions (e.g., NOs" and
NH4+)  should be monitored and assessed utilizing a
nationwide network of monitoring stations. EPA should
coordinate and inform its regulatory monitoring and
management of reactive nitrogen with the multiple
efforts of all agencies including those of the U.S.
Department of Agriculture and NSF-supported efforts
such as the National Ecological Observatory Network
(NEON) and the Long Term Ecological Research
Network (LTER). (Recommendation #5)
To ensure that urban fertilizer is used as efficiently as
possible, the Committee recommends that EPA work
with other agencies such as USDA as well as state and
local extension organizations to coordinate research
and promote awareness of the issue. (Recommendation
#7a)
Through outreach and education, supported by
research, improved turf management practices should
be promoted, including improved fertilizer application
and formulation technologies and maintenance
techniques that minimize supplemental Nr needs and
losses, use of alternative turf varieties that require less
fertilization, alternative ground covers in place of turf,
and use of naturalistic landscaping that focuses on
native species. (Recommendation #7b)
EPA should pursue the longer term goal of monitoring
individual components of Nr, such as NO2 (with
specificity), NO, PAN, and HNOs, and other inorganic
and reduced forms, as well as support the  development
of new measurement and monitoring methods.
(Recommendation #8c)
The scope and spatial coverage of the Nr concentration
and flux monitoring networks (such as the National
Atmospheric Deposition Program and the Clean Air
Status and Trends Network)  should be increased, and
an oversight review panel should be  appointed for these
two networks. (Recommendation # 8d)
EPA in, coordination with other federal agencies,
should pursue research goals including:
 • Measurements of deposition directly both at the
  CASTNET sites and in nearby locations with non-
  uniform surfaces such as forest edges
 • Improved measurements and models of convective
  venting of the planetary boundary  layer (the lowest
  layer of the atmosphere) and of long range transport
 • Improved analytical techniques and observations
  of atmospheric organic N compounds in vapor,
  paniculate, and aqueous phases
 • Increased quality and spatial coverage of
  measurements of the NH3  flux to the atmosphere
  from major sources especially agricultural practices
 • Improved measurement techniques for, and numerical
  models of, NOy and NHX species (especially
  with regard to chemical transformations, surface
  deposition and off-shore export and linked ocean-land-
  atmosphere models of Nr). (Recommendation #8e)
Research should be conducted on: best management
practices that are effective in controlling Nr, especially
for nonpoint and stormwater sources (including
land and landscape feature preservation); setting
Nr management targets that realistically reflect
these management and preservation capacities; and
constructing a decision framework to assess and
determine implementation actions consistent with
management goals. (Recommendation 15d)
The EPA should support cross-disciplinary and
multiagency research on the interactions of
climate  and Nr. To determine the interactions of
global biogeochemical Nr cycles and climate, the

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  Committee suggests that EPA follow a series of steps
  such as:
1. Select several likely scenarios for global climate from
  the IPCC report for the year 2050
2. Down-scale statistics or nest regional climate models
  within each of these global scenarios to generate
  meteorological and chemical fields (e.g., temperature,
  relative humidity, winds, precipitation, CC^) for a few
  years around 2050
3. Run several independent biogeochemical Nr models
  (earth system models that include air/water/land) for
  North America for  these years with current Nr and
  emissions and application rates
4. Rerun models with decreased Nr emissions/application
  to evaluate strategies for controlling impacts such as
  those described in this report. (Recommendation #19)

6.5 Conclusions and Observations
  Nitrogen is not only an essential resource for humans,
for example its use in food production, but also a
byproduct of essential processes such as combustion.
The increasing amounts of nitrogen that humans capture
through the Haber-Bosch process for useful purposes
and the increasing amounts of nitrogen released from
combustion also add increasing amounts of excess
reactive nitrogen (Nr) to  the environment. In the United
States, the production of crops receives 60% of the
new Nr inputs from anthropogenic sources, but also
accounts for almost the same proportion of total Nr losses
from terrestrial systems to air and aquatic ecosystems.
Nitrogen fertilizer use efficiency by crops seldom reaches
50% and may be 33% or lower. Fossil fuel combustion is
another major contributor of excess Nr in the production
of essential energy. However, the excess Nr that flows
from these activities is not benign. There are serious
negative impacts from excess Nr on both human health
and the environment.  Table 1 gives a range of examples
of these.

The nature of the problem
  Dealing with excessive reactive nitrogen is an
extraordinarily complex issue. Part of this relates to the
nature of nitrogen and its ability to change its form and
flow through different media, as evidenced conceptually
by the nitrogen cascade. Nitrogen's transformative nature
only increases the difficulty of dealing with its negative
aspects. Further, unlike the linear problems that society
has been more accustomed to dealing with in the past,
excessive Nr in the environment is in a class of problems
sometimes characterized as "wicked" (Batie 2008,
Kreuter 2004). For a  "wicked" problem;
  • There is not universal agreement on what the problem
    is - different stakeholders define it differently
  • There is no defined end solution, the end will be
    assessed as "better" or worse"
   • The problem changes over time
   • There is no clear stopping rule - stakeholders,
    political forces and resource availability will make
    that determination on the basis of "judgments"
   • The problem is associated with high uncertainty of
    both components and outcomes
   • Values and societal goals are not necessarily shared
    by those defining the problem or those attempting to
    make the problem better
   This is not to say that society does not try to deal with
such problems (healthcare, environmental degradation,
water resource management, food safety, etc.). What it
does say is that different approaches are needed to deal
with such problems as compared with better defined
problems that are amenable to disciplinary linear science
where experts can define the problem and its endpoint.
   The Integrated Nitrogen Committee has provided a
number of findings and recommendations with respect to
the problems caused by excess Nr. Some of these respond
to specific defined science based concerns. Others relate
to broader concerns the Committee raised in dealing with
the overall Nr dilemma. The following points synthesize
some of the important lessons learned from the more
than four years of study on this issue by the Integrated
Nitrogen Committee.
Recognizing the problem and building
consensus
   It is critically important that the problems caused
by integrated nitrogen be widely recognized. Many
recognize that we add nitrogen to the environment for
specific useful purposes. Fewer recognize that there are
both direct and indirect impacts from this that damage
both the environment and human health. Until there is
general recognition that there is a problem and we need
to deal with the negative externalities of excess reactive
nitrogen in our environment, there will be no willingness
to tackle this issue. Education, communication and
outreach are critically important to engender in the
public sufficient will to tackle this widespread problem.
Education, communication and outreach will be critical
to the formation of a common definition of the problem,
an essential step once it is recognized. Following that,
there will have to be some degree of public consensus
for actions that will effectively reduce excess reactive
nitrogen in the environment. These steps will not be
possible unless there is a process of public consensus
building with respect to Nr. The first essential step in
trying to deal with a "wicked" problem is getting some
measure of agreement across different participants and
stakeholders about the problem itself.

The importance of the nitrogen cascade
   Understanding the problem will require recognition
of the nature of Nr. In this case, the Integrated Nitrogen
Committee found the concept of the nitrogen cascade

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(pictured in the Executive Summary and Chapter 1)
to be an essential guide to approaching the problem.
This conceptual framework traces the flows of new
nitrogen through atmospheric, terrestrial, and aquatic
environmental systems where Nr is received, stored
and passed on in one form or another. The Committee
initially spent much of its effort on understanding
these flows and then determining as best as possible
the magnitudes of the flows and sinks through these
systems. This is the critical baseline information
needed to understand the problem, and many of the
recommendations in this report relate to gaining a better
understanding of this phenomenon. Unless it is well
understand how Nr flows through these systems, what
the Nr sinks are in these system components, and what
the magnitudes are of both the sinks and flows, any
attempts to better control Nr may well be ineffective.
Understanding and being able to quantify the nitrogen
cascade will allow the identification of the major excess
Nr contributors, an understanding of the different forms
of Nr at various points along the cascade, and enable
the determination of more effective interdiction points.
Without this basic understanding of the flows of Nr,  their
nature, and their magnitude, attempts to deal with excess
Nr will have high uncertainty. Chapter  2 summarizes
much of this critical information that the Committee was
able to determine and assemble from other sources.

Integrated approaches are essential
   Given what we know about the way Nr behaves,
efforts to deal with excess Nr must be organized in a way
that reflects the nature of the problem. Unfortunately,
many of our approaches are narrowly disciplinary
focused, and our policy and regulatory institutions are
often focused on one or another media where excess Nr
may temporarily reside or on a sector that contributes
excess Nr. The regulatory structure that has evolved for
problems affecting human health and the environment
has been specifically narrow, following policymakers
focus on such things as clean water or clean air. These
silos have to be broken down if excessive Nr is  to be
dealt with effectively. Current efforts by EPA and other
agencies to encourage more integration across these
silos, and include other institutions and stakeholders,
are absolutely essential. Research efforts to better
understand excess Nr and better mitigate its negative
impacts must be trans-disciplinary. What will also be
critical is greatly increased collaboration and cooperation
among and between agencies and interested stakeholders.
Several of the Committee's recommendations address
this issue, calling for formal mechanisms to encourage
this to take place.  The Nr problem has boundaries much
broader than the boundaries of the institutions that we
have relied on in the past to protect human health and
the environment. We are not going to be effective if we
do not both expand these boundaries and adopt a broad
multi-institutional reach.
Essential monitoring and research
   In the effort to understand and quantify the nitrogen
cascade the Committee became aware of areas of needed
research and monitoring. Much of this is essential for
improving our knowledge of what goes on in the cascade.
It is also essential for benchmarking current Nr flows and
sinks and for targeting where actions are best taken to
reduce excess Nr. There is not sufficient information and
understanding of these flows and sinks to  allow maximum
benefit from utilizing the full power of the cascade
concept. The Committee has recommended improved
monitoring and research to enhance our understanding
in air, land and water environments. In some cases
our knowledge has such wide margins of error that we
cannot identify or quantify important concentrations or
flows sufficiently for necessary  decisions. In other cases
we need much better understanding of the efficacy of
actions that might be taken to control Nr. In some cases
we need to know more about the indirect impacts of Nr
as well as the indirect impacts of measures to control
Nr. Monitoring is both an essential part of the research
needs as well as being a critical guide to what we face
and whether our efforts to better control Nr are being
effective. Such environmental monitoring is often not
considered to be critically important. It is critically
important for effective approaches  to reducing excess Nr.

Where to begin
   The approach taken in this report and its
recommendations should result  in helping enable the
control of excess Nr to proceed  on the basis of starting
where it is most technically and economically effective.
Some of this may be low hanging fruit, or particular
niches where institutions and stakeholders already have
a common purpose. Part of the value of the nitrogen
cascade is that its characterization of Nr sinks and
flows allows the comparison of alternative points of
entry and interdiction. Further monitoring and research
should also allow the comparison of different modes of
interdiction.  There will be choices to be made between
preventing increased Nr at the source, stabilizing it, or
treating the medium involved to remove it. Additional
research and actual experience in such efforts, coupled
with monitoring, will be essential for making these
choices. One product of the cascade exercise is a better
understanding of what and where the major contributors
of Nr are. The big sources of Nr have to be addressed
if there is to be meaningful impact on reducing excess
Nr. Part of the decision about where to begin will relate
to the efficacy of measures to reduce excess Nr, and our
knowledge is by no means complete on this. Beyond
the technical considerations of effectiveness, there will
have to be policy decisions about the trade-offs and
interdependencies between approaches such as market
mechanisms, regulation, incentives, and voluntary actions.
Market mechanisms generally require regulations that
are the basis for the creation of the  market situation that

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makes such things as emission trading viable. Voluntary
actions are more likely if there is some prospect of future
required action.

Metrics matter
   What is measured is critical to determining the
dimension of the problem, what to tackle, and whether
progress is being made. Kilograms removed (or prevented
at the source), the percentage of Nr removed from a
particular  cascade or sink, or dollars of damage avoided
by the removal of Nr are all reasonable measures to use.
Measuring the improvement in physical units of Nr can
lead to very different source targets, control measures and
estimations of success as compared to measuring dollars
of damage. This relates to the dilemma presented by
"wicked" problems in both the original problem definition
and the determination of whether actions have made
things better or worse. An example from the Chesapeake
Bay is cited in the report that illustrates this. If the goal is
less Nr in  the bay waters, then all sources are  important.
If one is concerned about reducing the economic damage
of Nr and  uses dollars of damage, then atmospheric
deposition becomes the primary focus because of the
high value of health damages from atmospheric pollution
related to Nr.
Setting goals for action
   Finally, nothing is going to be accomplished if goals
are not set and efforts do not get underway. There are
sufficient findings and recommendations presented by
the Committee in addition to what we already know to
enable multiple agencies to begin to reduce the excess
Nr entering the environment. Some of the trade-offs
between alternative approaches are also well known.
The Committee suggests actions that might be taken by
EPA or other management authorities to reduce nitrogen
in the environment and a 25% reduction of excess Nr is
suggested as attainable with current technology over the
near term. Actions being suggested need testing, refining
and require monitoring. A start, even on a pilot scale, in
one portion of the cascade or sector will yield valuable
information about the efficacy of the approaches used,
further demonstrate the necessity for a multi-agency joint
stakeholder approach, and help further define the problem
and where it can initially be best addressed. This necessity
of getting underway is one of the main recommendations
of the National Research Council's series of reports on
improving water quality in the Mississippi (National
Research Council, 2008b and 2009).  These reports
also emphasize the institutional arrangements that are
necessary. These are also echoed in this report. Following
on the National Research Council's reports, the USDA
Natural Resources Conservation Service has recently
begun an effort that targets nutrient reductions in the
Upper Mississippi.  It is time to get more efforts underway
with effective collaboration  between public and private
institutions and stakeholders.
   Because this report addresses the needs of EPA's
research mission, there is substantial emphasis on gaps
in knowledge about Nr and the research that needs to
be done to fill these gaps. However, the report contains
an extensive knowledge base about Nr which was a
necessary precursor to addressing the objectives of the
report. The report then identifies the problems posed
by Nr, assesses the  necessity of an integrated strategy
to deal with these problems, identifies some risk
management options for EPA's consideration, and makes
recommendations for improved research and monitoring
to support risk reduction. This information is sufficient
to allow initial determinations of where and how the Nr
problem can be addressed effectively with positive results.
As efforts progress, more will be learned and improved
methods, targeting, and analysis can be applied to this
truly wicked problem.

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Appendix A:
Nitrogen  Deposition  from  the Atmosphere to
the  Earth's  Surface
Review ofNr wet deposition
   Substantial progress has been made in monitoring Nr
wet deposition as is summarized in information provided
by the National Atmospheric Deposition Program/
National Trends Network (NADP) established in 1979.
This network monitors precipitation composition at over
250 sites in the U.S. and its territories (http://nadp.sws.
uiuc.edu). Precipitation at each station is collected weekly
according to well established and uniform procedures and
sent to the Central Analytical Laboratory for analysis of
acidity, NO3', NH4+, chloride, as well as the base cations
calcium, magnesium, potassium and sodium. For greater
temporal resolution, the Atmospheric Integrated Research
Monitoring Network AIRMON, composed of seven
sites, was formed in 1992 as part of the NADP program
to study wet deposition composition and trends using
samples collected daily. The same species are measured
as in NADP. By interpolating among sites, NADP is able
to estimate the wet deposition of NH4+ (reduced N), and
NO3~ (oxidized N)  for the 48 contiguous states (Table A-l
and Figure A-l).

   Table A-1: Annual wet deposition of reduced
   (NH4+), oxidized (NO3-), and total N to the 48
              contiguous states
NADP/NTN deposition estimates
reduced N in oxidized N in total wet N
precipitation, precipitation, deposition,
kg/ha/yr kg/ha/yr kg/ha/yr
1994
1995
1996
1997
1998
1999
2000
2001
2002
2003
2004
2005
2006
1.49
1.63
1.66
1.49
1.72
1.46
1.48
1.50
1.59
1.72
1.70
1.65
1.65
1.68
1.67
1.80
1.74
1.78
1.58
1.62
1.57
1.55
1.55
1.52
1.41
1.40
3.17
3.30
3.45
3.24
3.49
3.04
3.10
3.07
3.14
3.27
3.22
3.06
3.05
g  55
a  54
I  53
I 52
§ 51
    50
    49
    48
 '£  47
 I45
                     oxidized N in precipitation, %
                    • reduced N in precipitation, %
Source: NADP/National Trends Network (NTN) http://
nadp.sws.uiuc.edu
Figure A-1: Percent contribution of oxidized
(NO3~) and reduced (NHf) nitrogen wet deposi-
tion from 1994 to 2006.
As emissions of NOX have decreased, the relative
importance of NHX has increased (data from National
Atmospheric Deposition Program, 2010).

   Although individual regions vary, the NADP data for
the entire 48 states indicate an apparent decrease in NCV
wet deposition, but not in NH4+ deposition (Table A-l and
Figure A-2). Ammonium wet deposition shows a weak
increase, although the correlation coefficient is small. As
NOX controls have become more effective, the role of
reduced N appears to have grown in relative importance.
The nitrate data appear to show a statistically significant
trend and quantifying the response of deposition to a
change in emissions would be useful to both the scientific
and policy communities. A notable reduction in power
plant NOX emissions occurred as the result of the NOX
State Implementation Plan (SIP)  call (McClenny et al.,
2002; Gilliland et al., 2008; Bloomer et al., 2009). EPA
should pursue a rigorous analysis of the emissions and
deposition data, including identifying monitors and
methods that are consistent from the beginning to the end
of the record, as indicated in Recommendation 8.

How is Nr deposition related to emissions?
   The relationship between emissions of Nr and
observed deposition is critical for understanding the
efficacy of abatement strategies as well as for partitioning
local and large-scale effects of emissions. Only a few
studies covering several individual sites have sufficient
monitoring consistency and duration to determine
rigorously long-term trends in NCV and NH4+ and their
relationship to emissions, and here we consider several

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i.
1"
!M
SI
J
1
ft
•e
g
o
g
g

2.00
1.90
130
1.70
1.60
1.50
1.40
1.30
1.20
1.10
100


* * * *

*~ * * * *


y = 0.009 3x- 17.0
R! = 0.13
1993 1995 1997 1999 2001 2003 2005 2007
   2.00

   1.90

   1.80

   1.70

   1.60

   1.50

   1.40

   1.30

   1.20

   1.10

   1.00
*  *
Figure A-2: Trend in reported wet deposition of
NH4+ and NO3~ for the 48 contiguous states
Note the sampling methods and locations have not  been
tested for temporal or spatial bias (data from National
Atmospheric Deposition Program, 2010).
examples (Kelly et al, 2002; Butler et al., 2005; Likens et
al, 2005). These sites tend to be in the eastern U.S. where
monitoring is more concentrated and has a longer history
and where upwind sources and downwind receptors are
relatively well known. Examination of these studies
reveals that concentrations of gaseous and paniculate
N species in the atmosphere, as well as the Nr content
of precipitation over the eastern U.S., shows significant
decreases. Correlation with regional emissions is stronger
than with local emissions, in keeping with the secondary
nature of the major compounds - NCV and NH4+.
Decreases in NH4+ concentration and wet deposition are
attributed to decreases in SC>42~ concentrations, meaning
that more of the reduced Nr remains in the gas phase. For
the period 1965 to 2000, NCV  levels in bulk deposition
correlate well with reported NOX emissions. For shorter
and earlier time periods the correlation is weaker, and the
authors attribute this to changes in the EPA's methods of
measuring and reporting emissions; they find evidence of
continued errors in emissions from vehicles. Decreases in
deposition will probably not be linearly proportional to
decreases in emissions; for example a 50% reduction in
NOX emissions is likely to produce a reduction of about
35% in concentration and deposition of nitrate.
                The relationship between chemically reduced N
             emissions and deposition is more complex. The maps of
             ammonium deposition (Figure A-3) show that maxima
             occur near or downwind of major agricultural centers
             where emissions should be high. The full extent of the
             deposition record (see http://nadp.sws.uiuc.edu) shows the
             large intensification of NH4+ wet deposition in selected
             areas. The southeastern U.S., particularly North Carolina,
             has seen a long-term rise (Aneja et al., 2001; Aneja
             et al., 2003; Stephen and Aneja, 2008). The increase
             in deposition coincides with the increase in livestock
             production, but a swine population moratorium appears to
             have helped abate emissions (Stephen and Aneja, 2008).

                          Ammonium ion wet deposition, 2007
                            Nitrate ion wet deposition, 2007
                      Inorganic nitrogen wet deposition from nitrate
                               and ammonium, 2007
                                            *" i*
                                           i» „• • *-  -
                                                       &*
                                                      • :•  • o
                                                             -
                                                           U-4.D
                                                           U.I.D
                                                           tt-ta
                                                           ifl-ID
             Figure A-3: Annual NH4+, NO3-, and total
             inorganic N deposition for the year 2007 showing
             spatial patterns of deposition
             Source: National Atmospheric Deposition Program, 2010.

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Concentrations of aerosol NH4+ have decreased in many
parts of the country, and this may appear to contradict
the trend in wet deposition, but a decrease in condensed
phase NH4+ will be accompanied by an increase in
vapor phase NH3 if SO42~ and NO3" concentrations
decrease; see http://vista.cira.colostate.edu/improve/. This
potentially misleading information highlights the need for
measurements of speciated NHX (Sutton et al., 2003).
Review of dry deposition observations for  the
eastern United States
   Monitoring dry deposition presents a greater
challenge than monitoring wet deposition. The Clean
Air Standards and Trends  Network (CASTNET) and
Atmospheric and Integrated Research Monitoring
Network (AIRMON) were established to monitor
chemical and meteorological variables to infer dry
deposition in order to study the processes leading from
emissions to atmospheric concentrations and through
deposition to ecosystem effects. AIRMON dry deposition
monitoring was discontinued in 2003. See www.epa.gov/
castnet/, www.arl.noaa.gov/research/programs/airmon.
html, and http://nadp.sws.uiuc.edu.
   Recent reviews (Sickles and Shadwick, 2007a,b)
analyze the seasonal and regional behavior of
concentration and deposition of a variety of primary and
secondary pollutants including Nr and investigated trends
from 1990 to 2004 for the U.S. east of the Mississippi
River. The investigators evaluated observations from
more than 50 sites in the eastern states and concluded
that for 2000-2004, the mean annual total measured N
deposition for this area was 7.75 kg N per hectare per

 Table A-2: Deposition of N to  the eastern United
           States in units of kg N/ha/yr
  Dry NH4+
  Wet NH4+
  Dry HNO3 + NO3-
  Wet NO3-
  Total measured N Dep.
  Est. dry other NOy
  Est. dry NH3
  Est. total NO,,
  Est. total NH3 + NH4+
  Est. Grand Total
                                  ial deposition
                                     IM/h;
 0.41
 2.54
 1.E
 2.92
 7.75
 0.94
 1.90
 5.74
 4.85
10.59
Data are from the U.S. CASTNET program for the period
of 2000-2004. Monitored species for 34 sites east of the
Mississippi include vapor-phase HMOs, participate NOs',
and NH4+; unmonitored are other oxidized species such
as NOX and PAN and gas-phase reduced N species
most notably NH3 (Sickles and Shadwick, 2007a). For
an explanation of how deposition of unmeasured species
was estimated see text.
year (expressed as kg N/ha/yr); see Table A-2. This value
includes vapor phase HNO3, paniculate NO3", and NH4+;
it does not include deposition of other oxidized species
such as NOX and PAN, nor gas-phase reduced N species,
most notably NH3. The measured deposition rates peak
in spring and summer, but unaccounted for ammonia
deposition is probably a substantial fraction of the total,
and the true annual cycle remains uncertain.

Estimated total N deposition to the eastern
United States
   CASTNET monitors HNO3 and NCV, but not other
members of the NOy family - notably NOX. Dennis
(U.S. EPA, 2007d) estimated that the unmeasured NOy
species account for about 50% of the dry deposition of
nitrates. Half of 1.88 (see Table A-2) is 0.94 kg N /ha/yr.
Ammonia is also unmeasured by CASTNET, and model
estimates (Mathur and Dennis, 2003) of NH3 indicate
that dry deposition should account for 75% of wet NH4+
deposition; 75% of 2.54 is 1.9 kg N /ha/yr. Adding these
two values to the total from Table A-2 yields a reasonable
estimate, within about ±50% absolute accuracy, of total
deposition of about 10.6 kg N /ha/yr for the eastern U.S.

Characteristics of N deposition to the eastern
United States
   Analysis of production of N2 and N2O via gas phase
reaction is provided in Appendix E. Warmer temperatures
are conducive to release of NH3 from soils and manure
as well as from atmospheric particles, thus ammonia
concentrations are typically highest in summer. Diffusion
of gases is faster than diffusion of particles, and dry
deposition of vapor-phase Nr is faster as well; for
example the mean CASTNET reported HNO3 deposition
velocity is 1.24 cm/s while that for paniculate NO3~ is
0.10 cm/s.  In 2003 and 2004 substantial reductions in
emissions from electricity-generating units (power plants)
were implemented under the NOX State Implementation
Plan (SIP) call. Many of these power plants are
located along the Ohio River generally upwind of the
measurement area. Significant reductions (p = 0.05) were
found between the 1990-1994 and 2000-2004 periods
(Sickles and Shadwick, 2007a).

Uncertainty in measured deposition
   Analysis of uncertainties in the deposition of Nr is
challenging. The coefficient of variation for total regional
N deposition for 2000-2004 is 23%, representing a
minimal value of uncertainty. Concentrations of some of
the NOy species are monitored, as is the wet deposition of
major oxidized and reduced N species, but concentrations
of ammonia and other Nr species are not monitored. The
network for monitoring dry deposition is sparse and has
not been evaluated for spatial bias. The monitors are
located in flat areas with uniform surfaces - advective
deposition into, for example, the edges of forests are
estimated to contribute substantially to the uncertainty
(Hicks, 2006). Other sources of error include the model

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used to convert weekly average concentrations and
micrometeorological measurements into depositions.
Precision can be determined from collocated sites and
is estimated at 5% for nitrate and 15% for ammonium
in precipitation (Nilles et al, 1994). The uncertainty in
estimated dry deposition arises primarily from uncertainty
in deposition velocities (Hicks et al., 1991; Brook et
al.,  1997) and can be as high as 40% for HNO3. Total
uncertainty for deposition of Nr based on measurements is
at least 25% and may be as high as 50%.

Deposition estimates from numerical models
  The EPA Community Multiscale Air Quality model
(CMAQ) was run for North America at 36 km resolution
(R.  Dennis et al., personal communication, January 2008).
Simulation of Nr deposition is hampered by the lack of
emissions  information (especially for NH3), by the need
to parameterize planetary boundary layer (PEL) dynamics
and deep convection, as well as by simplified multiphase
chemistry. This run of CMAQ did not account for NOX
emissions  from marine vessels, and these amount to
about 4% of the total NOX emissions in 2000. Calculated
nitrogen deposition for the 48 contiguous states (Table
A-3) was broadly consistent with direct measurements
(Table A-2).  CMAQ NOX emissions were 5.84 Tg N for
the  year 2002; of that, 2.74 Tg N were deposited. This
suggests that -50% was exported - a number somewhat
higher than has been reported in the literature; this
discrepancy is discussed below.

     Table A-3: Results from CMAQ* for total
deposition in 2002 to the 48 contiguous states of
             oxidized and reduced N
kg N/ha/yr Tg N/yr
Oxidized N
Reduced N
Total N Deposition
3.51
2.66
6.17
2.74
2.07
4.81
The CMAQ results were adapted from Schwede et
al., (2009) The Watershed Deposition Tool: Atool for
incorporating atmospheric deposition in water-quality
analyses, htttp://www.epa.gov/amad/EcoExposure/
depositionMapping.html.

   Ammonia emissions and ambient concentrations can
be measured, but are not routinely monitored. For Nr, the
CMAQ numerical simulation employed inverse modeling
techniques - that is, NH3 emissions were derived from
observed NH4+ wet deposition (Gilliland et al., 2003;
Mathur and Dennis, 2003; Gilliland et al., 2006). Model
determinations of NH3 therefore do not provide an
independent source of information on NH4+ deposition.
   The three-year CMAQ run gives an indication of the
spatial pattern of deposition (Figures A-4). For NHX, wet
and dry are equally important, but for NOy, dry deposition
accounts for about two-thirds of the total deposition,
while wet deposition accounts for about one-third. While
this does not hold for the eastern U.S. it is true for the
U.S. as a whole; in arid southern California, for example,
dry deposition of Nr dominates. Based on CMAQ, total
NOy deposition is 2.79 times the wet deposition and total
NHX deposition is 1.98 times the wet deposition. Using
the data from Table A-l for the average wet deposition for
the period 2000- 2004, total deposition of oxidized N is
4.36 kg N /ha/yr (2.79  x 1.56 = 4.36). The total deposition
for reduced N is 3.17 kg N /ha /yr (1.98 x 1.60). The
grand total (wet and dry oxidized and reduced) is then
about 7.5 kg N /ha /yr.
   The model has highly simplified organic N deposition.
Note that these values reflect emissions before the NOX
SIP-call, which resulted in substantial reductions in NOX
emissions from point sources over the eastern U.S.
   For comparison purposes, a collection of chemical
transport models (CTMs) (Dentener et al., 2006) yielded
total (wet plus dry) deposition to the whole U.S. of about
3.9 Tg N /yr oxidized Nr and 3.0 Tg N /yr ammoniacal
N for current emissions. The fate of NOX is assumed to
be primarily HNO3 or aerosol NO3~; organic N species
are generally not modeled in detail. Because this analysis
includes Alaska, a better estimate for NOX for the 48
contiguous states is 4.6 Tg N /yr. The variance among
models was about 30% (one standard deviation) for
deposition fluxes in regions dominated by anthropogenic
emissions. Globally, the calculations from the ensemble
of 23 CTMs estimated that 36-51% of all NOy and NHX
emissions are deposited over the ocean. This load could be
important to estuarine N loading estimates, as offshore N is
carried inshore by currents or through advective processes.

Deposition estimates from mass balance
   From estimated total emissions of Nr compounds
and observed or simulated export, a reasonable estimate
of rate of deposition can be obtained by mass balance
- deposition equals emissions minus export. Although
substantial uncertainty (about a factor of two) exists for
the emissions of NH3, NOX release is reasonably well
known. In general, advection in the boundary layer and
lofting through convection followed by export at higher
altitudes are the two main mechanisms that prevent
removal of NOy and NHX by deposition to the surface of
North America (Luke et al., 1992; Li et al., 2004).
   Experimental observations have been conducted over
the eastern U.S. for more than two decades (Galloway
et al., 1984; Galloway and Whelpdale, 1987; Luke and
Dickerson,  1987; Galloway et al., 1988). Most recent
estimates (Dickerson et al., 1995; Li et al., 2004;  Parrish
et al., 2004b; Hudman et al., 2007), agree that annually
7-15% of the emitted NOX is exported in the lower to
mid-troposphere.
   CTMs derived small export values - on the order of
30% of the total NOX emitted into the lower atmosphere

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                              TOTAL NITROGEN DEPOSITION (KG-N/HA)
                                                    Hour: 00
                                         Min  0.18 at (40,8). Max- 36.41 at (93,29)
Figure A-4: CMAQ annual average (wet plus dry and oxidized plus reduced) nitrogen deposition (in
kg-N/ha/yr) across the United States.
This is based on three years of differing meteorology - one dry, one wet, and one average precipitation year - across
the Eastern United States
Source: U.S. Environmental Protection Agency, 2007f
(Penner et al., 1991; Kasibhatla et al, 1993; Holland
et al., 1997; Horowitz et al., 1998; Liang et al., 1998;
Galloway et al., 2004; Park et al., 2004; Holland et al.,
2005; Doney et al., 2007). Reviewed publications using
the mass balance approach have substantial uncertainty
but indicate with some consistency that 25-35% of the
NOy emitted over the U.S. is exported.

Comparison of models and measurements of
oxidized N deposition
  Both ambient measurements and numerical models
of NOy have reached a level of development to allow
reasonable estimates of deposition. For reduced
nitrogen, neither ambient concentrations nor emissions
are known well enough to constrain models. Recent
model estimates of the  U.S. N budget are reasonably
uniform in finding that about 25-35% of total NOX
emissions are exported.
  Results from CMAQ runs described above indicate
that, of the NOX emitted over the continental U.S., 50%
is deposited and 50% is exported. This is within the
combined error bars of other studies, but well under the
best estimate of 70% deposition. One possible source
of this discrepancy is underestimation of deposition of
organo-nitrogen compounds. The chemical mechanism
used in CMAQ was highly simplified - only about 2-3%
of the total Nr deposition can be attributed to organo-
nitrogen compounds (R. Dennis personal communication,
2008). Ammonia from fossil fuel combustion, while
important locally, is probably a small component of
national Nr deposition.
  Major sources of uncertainty in modeled and observed
values include missing deposition terms and poorly
constrained convective mass flux. As indicated above,
convective mass flux (rapid vertical transport) is uncertain
because most convective clouds are smaller than a grid
box in a global model. There is evidence for nonlinearities
in NO2 deposition velocities with greater transfer from the
atmosphere to the surface at higher concentrations (Horii
et al., 2004; 2006).

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   Emissions from Canada and Mexico can have a           emissions from Canada and Mexico are each 10-15% of
substantive impact on atmospheric Nr over the U.S. near     those of the U.S. and the bulk of the Mexican population
major sources, such as downwind of industrial Ontario       is distant from the U.S. We expect the overall impact of
and major cities of San Diego, CA, and Tijuana, Mexico     neighboring countries to add about 10% uncertainty to the
(Wang et al., 2009). While Nr is imported into the U.S.       estimated Nr budget for the 48 contiguous states.
from these border countries, there is also export. The

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Appendix  B:
Sources and  Cycling  of Nr  Input into
Terrestrial  Systems  in the  United  States
  Most of the new Nr introduced into terrestrial
systems in the U.S. was used to produce food for human
consumption and forage and feed for livestock and poultry
(-17.7 Tg total with 9.7 Tg from synthetic fertilizer and
~8 Tg from biological N fixation; Table B-l). In addition
to new Nr and Nr that was recycled from livestock and
human excreta, crop production releases Nr that was
stored in soil organic matter (see Section 2.3.2). The N
in cereal crops is typically derived from added fertilizer
(synthetic or manures) and from mineralization of soil
organic matter (conversion of complex organic molecules
to ammonium) in about equal amounts. As discussed
in Section 2.2 and Section 5.3.4, crop production is not
efficient in using Nr so only 30-70% (a global average
of 40%) of all the N mobilized for crop production is
harvested in the crop. The remainder is in crop residue
(roots and above ground stover) stored in the soil, leached
to aquatic systems as NCy, volatilized to the atmosphere
as NH3 or NOX or denitrified (see Section 4.8, Figure 18)
to produce NOX, N2O and N2. An additional ~1.1 Tg of
synthetic fertilizer N is used to maintain turfgrass in the
urban environment (see Section 2.2.5) and another 0.1-0.2
Tg N is used to enhance forest production.
                      Within the nitrogen cascade (Figure 1), the interactions
                    between the agricultural and populated portions of the
                    terrestrial system dictate the production and flow of Nr.
                    Although occupying the largest area, forest and grassland
                    portions of terrestrial ecosystems serve mainly to absorb
                    atmospheric deposition and provide a source of forest
                    products and forage for livestock production. Reactive
                    nitrogen input into these systems is from biological N
                    fixation in unmanaged lands, atmospheric deposition, and
                    Nr from livestock manure that is deposited. The livestock
                    that is grazing within grasslands (Table B-l) may lead
                    to the N saturation of unmanaged forest and grassland
                    ecosystems (Galloway et al., 2004; Bobbink et al., 2010).
                      This report uses the Nr input numbers from Table  B-l
                    and food production numbers to estimate the flow of Nr
                    through agricultural and populated parts of the terrestrial
                    system (Table B-2). The FAO (200lOb); www.fao.org/
                    es/ess/top/country.html) lists the 20 largest agricultural
                    commodities produced, imported, and exported in the
                    U.S. in 2002. Of these commodities, corn (229 Tg),
                    soybeans (75 Tg), wheat (44 Tg), and cow's milk (77 Tg)
                    were produced in the greatest amount. Using commodity
                    N content data derived from data used to calculate crop
   Table B-1: Sources of reactive N into terrestrial systems in the United States in 2002 (from Table 1
                                     data sources; in Tg N/yr).
      Atmospheric
       N fixation
      Synthetic N
      Animal manure##
       Human sewage##
1.3
7.7
9.7
1.2
0.1
1.4
0.1
1.9
             6.4
            3.8#
0.4
             1.1
                           1.2
6.9*
                         14.1
            10.9
                         6.0#
                          1.3
The amount of atmospheric Nr deposition is based on area of each environmental system within the continental U.S.
The total area does not sum to 100% because non-arable lands are not included in this table.
"""Synthetic fertilizer N used for managed pasture fertilization is included in the agricultural land classification.
#Unrecoverable livestock manure deposited on grasslands, the unaccounted for ~1 Tg of Nr assumed to be lost
through ammonia volatilization, leaching, or denitrification (U.S. EPA, 2007e).
##Note that livestock manure and human sewage used as fertilizer are recycled N components of the nitrogen cascade
and not new Nr inputs.

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residue N in the EPA inventory of U.S. greenhouse gas
emissions and sinks (U.S. EPA, 2007e), an estimated 9
Tg of N was marketed in three crops, soybeans (4.4 Tg
N; from U.S. EPA, 2007g), corn (3.2 Tg N), and wheat
(0.9 Tg N). Whole milk contained ~ 0.5 Tg of N while
other meat and egg produce contained ~1.4 Tg of N,
totaling ~ 1.9 Tg N. Grain, fruits, nuts and vegetables
contained ~9.3 Tg of N. If the total N input use efficiency
is 40%, then ~23 Tg of N from all sources is required to
produce 9.3 Tg of vegetative commodities. Table B-2
lists the estimated Nr input into agricultural systems (~
20 Tg) and additional N input from crop residue that was
returned to the field the previous year (4.4 Tg) and from
mineralization of soil organic matter (4.7 Tg). All of this
N input totals ~29 Tg of N that is actually involved in
the production of the 9.3  Tg of crop commodity N. If one
assumes that return of crop residue to the field is directly
proportional to crop production, then 24.3 Tg of N was
required to produce the 9.3 Tg of crop commodity N.
These estimates indicate that -38% of the total annual
input of N that went into  the agricultural crop production
system was contained in the main crop commodities
produced in the U.S. in 2002.
   Of the 24.3 Tg N required to produce crop commodity
N in 2002, approximately 2.5  Tg was used to grow feed
used for milk, egg, and meat production. This estimate is
made assuming that 4 units of N are required to produce
a unit of milk, eggs, or meat (see  Section 5.3.4). This
estimate also assumes that one-third of N required for
livestock production comes from commodities in the
FAO top-20 list and the remaining two-thirds comes
from alfalfa, silage, and grass over the course of a year
(Oitjen and Beckett,  1996). Approximately 4.3 Tg of N in
agricultural commodities (2.8 Tg in soybeans, corn, and
wheat) were exported, while ~0.15 Tg N was imported
in various food and drink commodities. The U.S. human
populace consumed —1.96 Tg of N in 2002 (292 million
people, consuming 114.7 g protein/person/day, 0.16 gN/g
protein, 365 days) (approximately 1.2 Tg from animal
protein-N and 0.7 from vegetative protein).
                                These three consumption areas - internal consumption
                              of vegetable N for livestock production, human
                              consumption,  and export - account for 77% of the
                              commodities produced. The unaccounted for commodity
                              N is likely partly in annual storage. Some smaller fraction
                              of annual production is used for pet food and a small
                              fraction is returned to the terrestrial environment because
                              of spoilage and handling losses.
                                In forests and grasslands (vegetated system) N input
                              in 2002 was -3.5 Tg of anthropogenically introduced
                              N, with the remaining —10.1 Tg derived from BNF and
                              livestock manure deposition. Of this anthropogenic N,
                              -21% was retained in soil and tree biomass, while the
                              remainder was removed in tree harvest (-0.2 Tg, see
                              Section 2.3.2.) or lost to other parts of the environment
                              through NH3 volatilization and NO3   leaching and runoff
                              (Table B-2). Total N input into agricultural systems
                              was -20 Tg, with - 11 Tg being removed as products
                              including the transfer of-2 Tg N as food to the human
                              population. Almost 40% of the N input into agricultural
                              systems is lost through NH3 volatilization, nitrification/
                              denitrification, and NO3" runoff. The 4.2 Tg of Nr of
                              Haber-Bosch N that is used for industrial feedstock is
                              not included in this assessment. Of the input of-3.3 Tg
                              of N into the populated system -80% is  lost through
                              human excreta processed in sewage treatment plants,
                              denitrification in soils, and leaching and runoff of NO3~
                              (Table B-2).
                                Table B-2 summarizes the input and flow of Nr in
                              the main terrestrial systems within the continental U.S.
                              Anthropogenic input of Nr into forests and grasslands
                              totaled -3.5 Tg in 2002, with an estimated 6.4 Tg of Nr
                              being introduced through natural biological N fixation.
                              Of this Nr - 0.7  Tg was stored in vegetation and soils
                              (see Section 2.3.2) and -2 Tg removed as livestock
                              forage, while the remainder was lost to the atmosphere
                              and aquatic systems, or removed as forest products and
                              livestock forage. The largest anthropogenic Nr input (-20
                              Tg) was into agricultural production where -11.2 Tg was
                              removed as agricultural product, - 2 Tg  transferred as
   Table B-2: Nr input and flows (Tg N/yr) in the terrestrial portion of the Nitrogen Cascade (Figure 1)
                               within the continental United States in 2002
 Vegetated
13.6
0.7
                                                          Icultural &   Transfers to
                                                                             Aquatic or
                                                                          . .xmospheric
 2.2
10.7
79
 Agricultural
19.6
0.8
11.2
 7.6
39
 Populated
 3.3
0.1
   0
 3.2
97
The Environmental Systems are those noted in the terrestrial portion of the N cascade shown in Figure 1. Data from
Table B-1, derived from regrouping information from Table 1 data sources, are shown in Table B-2.
"""Estimates are from section 2.3.2 of this report.

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edible product to the "populated" portion of the terrestrial
system, -0.8 Tg was stored in agricultural lands, and -7.6
Tg N was lost to the atmosphere and aquatic systems.
New N input into the "populated" portion totaled ~3.3 Tg,
which came from N transfer in food and use of fertilizer
N in lawns, gardens and recreational areas. Within these
areas an estimated 0.12 Tg was stored in urban forests.

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Appendix C:
Water Quality  Trading  in  the  Illinois
 River  Basin
   For various reasons, wetland restoration has been
proposed and the magnitude of needed restoration
estimated. For the Wetland Reserve Program (WRP), the
Farm Bill of 1990 set a goal of restoring approximately
1 million acres. A few years later, the NRC (1992)
proposed a national goal of restoring 10 million acres of
inland and coastal wetlands by 2010. The NRC went on
to recommend that 400,000 miles of streams and rivers
be restored by 2012 and that 1 million acres of lakes
be restored by 2000, both of which would further the
control of reactive nitrogen. While none of these goals
has been or is likely to be met by the recommended
date, they articulated a need for wetland restoration
addressing the important relationship between wetlands
and water quality.
   Taking into account the economics of using wetlands
to manage Nr adds yet another dimension to site
selection. Based on the results of the Water Environment
Research Foundation's study (Hey et al., 2005a,b), the
Kinship Foundation sponsored a study (Scott et al., in
preparation) to define the market for producing and
selling Nr (as NO3~) credits. For this analysis, a real
potential market area was selected: the Illinois River
watershed in Illinois - the tributaries draining Wisconsin,
Indiana, and Michigan were excluded. The producers
of nitrogen credits were identified as "nutrient farmers"
and they became the "sellers" of N credits. The "buyers"
of nitrogen credits were restricted to municipal and
industrial wastewater treatment facilities, those facilities
that hold an NPDES permit. This restriction, of course,
resulted in a considerable understatement of the market
size since the identified buyers emit less than 11% of the
total aquatic N load (David and Gentry, 2000) that finds
its way to the Mississippi River - air emission/deposition
and agriculture account for the remaining 89%.
   The watershed was divided into  19 sub-watersheds,
spatially locating credit supply and demand. A linear
programming model was developed and used to:  (1)
examine the potential extent and distribution of nitrogen
credit demand and supply; (2) compare the average
seasonal demand levels to the supply capacity of nutrient
farms; and (3) evaluate the relative effects of seasonality.
Market efficiency was imposed through the objective
function: the least costly distribution of credit production
to meet the given monthly demand. Thereby, sellers
and buyers were identified and linked and the spatial
characteristics of the market mapped by sub-watershed.
At the same time, the equilibrium price of a credit, or the
prevailing price at which buyers and sellers are willing
to trade, was determined. The market, as represented by
the model, determined where the most intensive wetland
investment (i.e., wetland restoration) would be, the
revenues returned to these investments, and the costs and
savings to the buyers.
  All 290 permitted buyers are geographically
distributed as shown in Figure C-l. The mass loading
of the buyers (2,423 tons/month) is reflected in Figure
C-2. Eighty-nine percent of the demand comes from the
northeastern corner of the Basin (Upper Fox, Des Plaines,
and Chicago/Calumet sub-watersheds), the Chicago
metropolitan area. As illustrated by Figure C-3, 41% of
the wetland restoration area (using the criteria discussed
above) was identified in the southwestern corner of the
watershed (Lower Illinois, La Moine, Macoupin, Lower
Sangamon, and Middle Illinois sub-watersheds), where
the floodplain is almost entirely leveed. For the market
study, the available load of Nr (NCV) by season and
sub-watershed was mapped as illustrated in Figure C-4.
The N load was computed using water quality and flow
data collected by the U.S. Geological Survey from 1987
to 1997. The wetland and wastewater cost functions are
described in Hey et al., 2005; however, the wetland cost
functions were modified for the market study to reflect the
variability of land costs across the watershed (i.e., higher
land values in urban Chicago and lower land values in
rural Illinois). This variability is reflected in the spatial
distribution marginal costs  shown for the spring marginal
costs depicted in Figure C-5. Wetland treatment costs vary
by time of year because the level of microbial activity,
which drives the denitrification process, varies with water
temperature. Therefore, treating an equivalent load of Nr
requires more wetland area in winter than in summer.

Three regulatory scenarios
  Regulatory agencies may require that dischargers and
nutrient farms be located in proximity to each other and
could impose "penalties" when the two are not. Thus,
for the sake of analysis, the Committee created three
regulatory scenarios:
1. Unrestricted - buyers can purchase nitrogen credits
  from nutrient farmers anywhere in the watershed
  without regard to location. The result of this scenario is
  given in Figure C-6.
2. Restricted intra-watershed - the buyer must purchase
  all available credits within its own sub-watershed
  before buying in other sub-watersheds

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3. Accrued 10% penalty - buyers pay an increasing
  "tax" on credits purchased in consecutive downstream
  watersheds
   The three regulatory scenarios were analyzed for each
of the four seasons. (D. Hey, Wetlands Research, Inc.,
Personal Communication.)
   The "unrestricted" scenario is the least expensive
because nutrient farms in this scenario are located
downstate, where land is least expensive. In the other two
scenarios, credits were purchased a little more evenly
throughout the watershed. Still, most of the credits in the
southern corner of the watershed were purchased. The
    Industrial Dischargers
    Major Municipal Dischargers
    Minor Municipal Dischargers
    River
    County Boundary
 Q Watershed Boundary
Figure C-1: Distribution of municipal discharge
and industrial dischargers in the Illinois River
Watershed
Municipal dischargers shown are those exceeding one
million  gallons per day. Symbols may represent more
than one discharger at that location.
Source: D. Hey, Wetlands  Research, Inc., Personal
Communication
                                                         A/ River
                                                            Watershed

                                                        Acres Available
                                                        (thousands)
                                                              7-15
                                                             15-25
                                                             40-65
                                                             65-120
Figure C-3: Potential land availability in the
100-year flood zone for nutrient farming in each
sub-watershed in the Illinois River watershed
Source: D. Hey, Wetlands Research, Inc., Personal
Communication
  A/ River
     Watershed
  Tons of Nitrogen
       1-5
       6-20
      21-35
  • 36-100
     B101 -350
     351 -1,750
Figure C-2: Distribution of total nitrogen
emissions by sub-watershed
Source: D. Hey, Wetlands Research, Inc., Personal
Communication
      River
   I—I Watershed

  Tons of Nitrogen
        0-50
       51 -125
   • 126-275
   • 276 - 400
   ••401-750
   • 751-1,500
Figure C-4: Spring available total nitrogen load
by sub-watershed
Source: D. Hey, Wetlands Research, Inc., Personal
Communication

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       iver
  l~~l Watershed

  Cost
  dollars par ton
     1,000-1,950
     1,951 -2,100
     2,101 -2,250
     2,251 - 2,500
   • 2,501 - 2,750
  •• 2,751 - 3,000
Figure C-5: Spring marginal cost (price) by
watershed
Source: D. Hey, Wetlands Research, Inc., Personal
Communication
"restricted intra-watershed" and "accrued 10% penalty"
scenarios resulted in more credits being purchased. This
resulted in the sale of N credits exceeding the mass
of Nr emitted by wastewater treatment, which would
benefit the overall control of reactive nitrogen. It also
would increase the value of the market and the profits
of the nutrient farmer. The downside of such regulatory
controls is that they would drive up the effective price of
nitrogen credits. If a buyer had to buy a 1.5 tons for every
ton discharged because credits are not available in the
tributary watershed, the effective price of a credit would
be 1.5 times the price of the tributary sub-watershed. If
prices rise too much, "concrete and steel" technologies
may become competitive.
   Considering all of the point source dischargers
in the Illinois River watershed, between 29,000 and
36,000 tons TN/year could be removed through nutrient
farming under the studied trading schemes (Table C-l).
The range of removal is a function of the penalties
imposed on the market by the regulatory agencies.
                   V River
                  CH Watershed
                  Tons of Nitrogen
                        0
                        1 -200
                       201 -400
                       401 -1,000
                   • 1,001 -1,500
                  §•1501-2000
                Figure C-6: Unrestricted spring credit sales
                (tons/month) by sub-watershed

                Source: D. Hey, Wetlands Research, Inc., Personal
                Communication

                Accordingly, the market revenue would range from
                $70 million to $121 million/year. This is a sizeable
                market that could generate substantial profits, from $6
                million to $38 million with the return on investment
                varying from 5 to 25%. If the savings are shared evenly
                between the seller and buyer, the nutrient farmer could
                earn between $200 and $300/acre/year net profit, which
                in many cases is greater than the profits from corn
                or soybean production.  Further, these profits do not
                include any earnings from flood control or recreation, as
                suggested in a McKnight study report (Hey et al., 2004).
                With such profits, sufficient land should be available for
                nutrient farming.
                  This analysis indicates that appropriate lands
                are available and that wetlands can be effectively
                restored and efficiently  used to control Nr. The market,
                structured as discussed  above, could generate the capital
                to accomplish the needed large-scale wetland restoration
                while saving taxpayers  the cost of upgrading their
                municipal wastewater treatment plant (TWI, 2007).
               Table C-1: Nutrient farm market parameters under three trading scenarios
 Parameter
 Total Credits Sold (tons)
 nrestnctei
   29,078
Restricted
ra-watershe
   29,078
  :crued 10%
  Penalty
   35,781
 Total Revenue 21
$69,925,497
$99,571,889
$121,457,652
 Total Cost to Produce Credits
$63,258,006
$66,193,924
 $83,288,747
 Profit
 $6,667,491
$33,377,968
 $38,168,905
Source: D. Hey, Wetlands Research, Inc., Personal Communication
21 Assumes all credits were sold at the cheapest cost within the Illinois River watershed.

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Appendix D:
Management  of Nr Measures  Based  on  the
Concept of  Critical  Loads
  The European Union has undertaken broad measures,
based on the critical-loads concept, to manage Nr.
Tables D-l, D-2, and D-3 summarize several different
environmental impacts currently used as indicators
and identify whether there are current limit values
set by the United Nations Economic Commission for
Europe (UNECE) or European Union (EU). These
tables identify the main links to the cascade of reactive
nitrogen in the environment, the relevance and link to
Nr of the effect/pollutant, and existing agreements in
which the effect is currently addressed. In addition,
some impacts are more relevant than others in relation
to societal importance and the connection to the nitrogen
cascade. The categorization on a scale of 1 (highest
relevance) to 5 (unimportant) provides a first-level
prioritization for future mitigation activity. The last
column summarizes existing links to international
regulations and conventions.
  Where there is a limit and the relevance for the
nitrogen cascade is high, then this might be the limiting
factor for Nr production and its associated losses to the
environment. Some limits might be more relevant in
specific areas and less relevant in others. For example
NO2 concentrations relevant for human health are
limited to 40 ppb in urban areas, limiting industry and
traffic, but would probably not be an issue of concern
in remote areas with low population densities. In
these areas, however, loss of biodiversity might limit
nitrogen deposition and therewith the sources in the
region. The only way to determine the extent that
critical thresholds are limiting is by overlaying them
on different regions and determining, through the use
of monitoring data or by modeling exercises, where
and which sources contribute to exceeding the critical
threshold. Then the best methods for putting caps on
relevant sources can be identified. A pre-classification
of regions might be useful, e.g., urban regions, remote
regions, marine areas, etc. One aspect of this global
view of nitrogen impacts and metrics that is evident is
the mix of "classical-" and "service"-based categories,
consistent with the need for an integrated approach to
the management of nitrogen.

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    Table D-1: Summary of the effects of excess Nr on human health in relation to metrics, current
           international regulations and conventions, and the link to the nitrogen cascade


Respiratory disease in people
caused by exposure to high
concentrations of:
Ozone
Other photochemical
oxidants
Fine particulate
aerosol
Direct toxicity of nitrite
NO2-
Nitrate contamination of
drinking water
Depletion of stratospheric ozone
Increase allergenic pollen
production, and several
parasitic and infectious human
Blooms of toxic algae and
decreased swimability of
in-shore water bodies
^^^^T-^TVrV^^I


Sum of ozone
over 35 ppb
Org. N03, PAN
concentration
(atm)
PM-io, PM2 5
concentration
(atm)
NO2-
concentration
N03-
concentration
(aq.)
NOX, N20
concentration/
flux (atm)

Chlorophyll a
NO3- (&P)
concentration
(aq)




YES
NO
YES
YES
YES
NO
NO
NO
Link to Nr


NOX
emissions
NOX
emissions
NOX, NH3em
NOX
N03-
leaching
NOX, N20

Runoff, Nr
deposition




3
5
1
2
2
3
5
1
Regulatory or
political
JBK

Convention on Long-
range Transboundary Air
Pollution
Clean Air for Europe
Convention on
Long-range
Transboundary Air
Pollution et al.
Convention on Long-
range Transboundary Air
Pollution
Clean Air for Europe
World Health
Organization
Convention on Long-
range Transboundary Air
Pollution
Clean Air for Europe
EU Essential
Facilities Doctrine
Montreal Protocol
None
Convention for the
Protection of the Marine
Environment of the
North-East Atlantic
Helsinki Commission
Barcelona Convention
atm - atmospheric; aq - aqueous

*Relevance and link to nitrogen incorporates societal priority and N contribution: 1. highest relevance, 2. high
relevance,  3. significant relevance, 4. some relevance, 5. unimportant.

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 Table D-2: Summary of the effects of excess Nr on ecosystems related to currently used metrics, the
            existence of European regulatory values, and the link to the nitrogen cascade.

Ozone damage to crops,
forests, and natural
ecosystems

Acidification effects on
terrestrial ecosystems, ground
waters, and aquatic
ecosystems
Eutrophication of
freshwaters, lakes
(incl. biodiversity)

Eutrophication of coastal
ecosystems inducing
hypoxia (incl. biodiversity)




Nitrogen saturation of soils
(incl. effects on GHG
balance)

Biodiversity impacts on
terrestrial ecosystems
(incl. pests and diseases)



Me,rics
AFstY(O3flux),
AOT40"

Critical loads
Biological
Oxygen De-
mand,
NO3~ cone (aq)
Critical loads
BOD, NO3- cone
(aq)
Critical loads




Critical loads

Critical loads,
critical levels
(NH3, NOX)



^g
YES

YES
YES
NO

BOD, N03-
conc (aq)
Critical load




YES

YES



Link to Nr
cascade
NOX

Nr deposition
Runoff, Nr
deposition

Runoff, Nr
deposition




Nr deposition

Nr deposition




2

2
3

1




1

1



Regulatory or
political
convention
Convention on Long-
range Transboundary
Air Pollution
Clean Air for Europe
Convention on Long-
range Transboundary
Air Pollution
Clean Air for Europe
WFD
Water Framework
Directive

Convention for the
Protection of the
Marine Environment of
the North-East Atlantic
Helsinki
Commission
Barcelona
Convention
Convention on Long-
range Transboundary
Air Pollution
Clean Air for Europe
Convention on Long-
range Transboundary
Air Pollution
Clean Air for Europe
Convention on
Biological Diversity
atm - atmospheric; aq - aqueous
*Relevance and link to nitrogen incorporates societal priority and N contribution: 1. highest relevance, 2. high
relevance, 3. significant relevance, 4. some relevance, 5. unimportant.
"""Accumulated ozone exposure over a threshold of 40 parts per billion

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      Table D-3: Summary of the effects of excess N on other societal values in relation to metrics
     and regulatory values in current international regulations and conventions and the link to the
                                            nitrogen cascade
                          Metrics
                                                                          \\atory or
                                                                          >litical
                                                                          ventio
 Odor problems as-
 sociated with animal
 agriculture
Acidity in precipitation.,
        03,

        PM
                                              YES
NOX, NH3
   Convention on
    Long-range
  TransboundaryAir
      Pollution
 Effects on
 monuments and
 engineering
 materials
  PM2 5 cone (atm)
                                              NO
NOX, NH3
 Global climate
 warming induced by
 excess nitrogen
 N2O, cone/flux (atm)
                                              NO
NOX, NH3
   United Nations
     Framework
Convention on Climate
      Change
 Regional climate
 cooling induced by
 aerosol)
  PM2.5 cone (atm)
                                              NO
NOX, NH3
   United Nations
     Framework
Convention on Climate
      Change
atm - atmospheric; aq - aqueous
*Relevance and link to nitrogen incorporates societal priority and N contribution: 1. highest relevance, 2. high
relevance, 3. significant relevance, 4. some relevance, 5. unimportant.

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Production of A/2 and A/2O via gas-phase
reactions
   Atmospheric conversion of NOX and NHX to less
reactive N2 or N2O appears to play a minor role in the
global N budget, but currently is not well quantified. The
gas-phase reactions in the troposphere that convert NH3
and NOX to N2 and N2O, start with attack of NH3 by OH:
           NH3 + OH-^NH2-+H2O   (1)
   Several potentially interesting fates await the NH2
radical:
           NH2-+O3^NH,NHO,NO  (2)
           NH2 • + NO2 -> N2O + H2O   (3)
           NH2-+NO^N2 + H2O      (4)
                 kO3 = 1.9xlO-13 cm3 s'1
                kNO2=1.8xlO-11cm3s-1
                kNO=1.8xlO-11cm3s-1
   The first step, attack by OH,  is slow. The rate constant
for the Reaction 1 is 1.6 x 10"13 cm3 s"1 and the lifetime
of NH3 for a typical concentration of 106 OH cm'3
is about 70 days. In most areas of the world where
concentrations of NH3 are high, concentrations of sulfates
are also  high, and NH3 is removed by conversion to
condensed phase ammonium sulfate or bisulfate on time
scales much faster than 70 d.  The mean lifetime of these
aerosols with respect to wet deposition is about 10 d.
   There are some areas of the world, notably  California
and South Asia, where NH3 and NOX are emitted in large
quantities, but SO2 is not, and there gas-phase conversion
can take place. NH3 is usually removed by wet or dry
deposition. Reaction 2 is relatively slow and oxidation of
ammonia is in general an unimportant source of NOX. But
Reactions 3 and 4 may be atmospherically noteworthy. As
an upper limit to current N2O  production, we can assume
that each of these regions covers an area of 106 km2 and
that they contain ammonia at a concentration of 10 g N nr3
in a layer 1,000 m deep. The annual production of N2 and/
or N2O would then be on the order of 0.1 Tg N, a minor
but nontrivial contribution to denitrification and about 1%
of the anthropogenic N2O production. If NH3 rich air is
lofted out of the boundary layer into the upper troposphere
where deposition is impeded, it will have an atmospheric
residence time on the order of months, and the probability
of reaction to  form N2O or N2 becomes greater. This
possibility has not been investigated extensively. It is also
possible than Europe and North America will continue to
reduce S emissions without reducing NH3 emissions and
the atmospheric source of N2O will grow in importance.
   In the stratosphere, N2O photolysis leads to loss of
Nrvia
N2O
                          N2 + O   (5)
   While reaction with an electronically excited oxygen
atom O(1D) leads to production of NO via
              N2O + O(!D) -> 2NO  (6)
   Photolysis (Reaction 5) dominates, but a large
enough fraction of the N2O reacts with O^D) that this
is the main source of NOX in the stratosphere. The fate
of this oxidized nitrogen (NOy) is transport back into
the troposphere where it is removed by wet deposition.
Downward transport of the odd N from the oxidation of
N2O is a minor (~1%) source of NOy in the troposphere.
Most of the N2O released into the atmosphere is
eventually converted to N2 - the problem is that it
destroys stratospheric ozone in the process.
   In summary, our current understanding of the
chemistry of atmospheric ammonia suggests that in situ
conversion to N2 and N2O plays a minor (~1%) role
in global N budgets, but if assumptions about kinetics
or concentrations are in error, these mechanisms could
become important.

SPARROW model for estimating watershed Nr
   Estimates of Nr transfers in aquatic ecosystems are
difficult to quantify at the national scale, given the need
to extrapolate information from sparse monitoring data
in specific watersheds to the geographic boundaries of
the nation. One excellent tool for estimating Nr loads at
regional scales is the spatially referenced regression on
watershed attributes (SPARROW) modeling technique.
The SPARROW model has been employed to quantify
nutrient delivery from point and diffuse sources to
streams, lakes, and watershed outlets at the national scale
(Smith etal., 1997). The model infrastructure operates
in a geographic framework, making use of spatial data
to describe sources  of pollutants (e.g., atmospheric
deposition, croplands, fertilizers) and characteristics of
the landscape that affect pollutant transport (e.g., climate,
topography, vegetation, soils, geology, and water routing).
Though empirical in nature, the SPARROW modeling
approach uses mechanistic formulations (e.g., surface-
water flow paths, first-order loss functions), imposes mass
balance constraints, and provides a formal parameter
estimation structure to statistically estimate sources and
fate of nutrients in terrestrial and aquatic ecosystems. The
spatial referencing of stream monitoring stations,  nutrient
sources, and the climatic and hydrogeologic properties
of watersheds to stream networks explicitly separates

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landscape and surface-water features in the model. This
allows nutrient supply and attenuation to be tracked
during water transport through streams and reservoirs,
and accounts for nonlinear interactions between nutrient
sources and watershed properties during transport. The
model structure and supporting equations are described
in detail elsewhere (Smith et al, 1997, Alexander et al.,
2000, Alexander et al., 2008).  Figure E-l provides an
estimate of contemporary Nr loading in surface waters
of the U.S., representing long-term average hydrological
conditions (over the past three decades). There are hot
spots of high Nr yields to rivers associated with land use
and watershed characteristics, and SPARROW allows
considerations of the fate of these Nr inputs to streams
and rivers as they flow downstream to coastal receiving
waters (Alexander et al., 2008).
                                                                                0-5
                                                                                6-10
                                                                                11-17
                                                                                18-26
                                                                                27-50
                  Figure E-1: Total Nr yields (kg/ha/yr) in large rivers of the U.S.
                  Data Source: Alexander et al., 2008

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Appendix F:
Recent Major  EPA  Mobile  Source  Rules to
Control  NOX
  EPA informed the Committee that it is in the process
of implementing a number of regulations to reduce NOX
from a variety of mobile sources22. These include clean
diesel regulations for trucks and buses and nonroad
engines, as well as locomotives and smaller marine
vessels. EPA first regulated NOX emissions from motor
vehicles for the 1973 model year and since then has
tightened these standards. EPA's efforts to control NOX
emissions from nonroad vehicles, locomotives, and
commercial marine vessels started in the 1990s. NOX
reductions for each rule were calculated by EPA based
on inventories available at the times of the rules.
1. Light Duty Tier 2 Rule - EPA's Tier 2 Vehicle and
  Gasoline  Sulfur Program (65 FR 6698, February 10,
  2000). This program requires new cars, sport utility
  vehicles (SUVs), pickup trucks, and vans to be 77
  to 97% cleaner than 2003 models, while reducing
  sulfur levels in gasoline by 90%. EPA estimates that
  as newer, cleaner cars enter the national fleet, the new
  tailpipe standards will reduce emissions of nitrogen
  oxides from vehicles by 3  million tons, or about
  74% in 2030. Prior to that, the EPA Tier 1 vehicle
  regulations, effective with the 1995 model year, also
  resulted in significant NOX reductions.
2. EPA's Clean Heavy Duty Truck and Bus Rule. When
  the Agency finalized the Heavy Duty Truck and Bus
  Diesel Rule (66 FR 5002, January 18, 2001) in 2001,
  trucks and buses accounted for about one-third of
  NOX emissions from mobile sources. In some urban
  areas, the contribution was even greater. With model
  year 2010, all new heavy duty trucks and buses will
  result in NOX emission levels that are 95% below
  the pre-rule levels. EPA projects a 2.6 million ton
  reduction of NOX emissions in 2030 when the current
  heavy-duty vehicle fleet is completely replaced with
  newer heavy-duty vehicles that comply with these
  emission standards.
3. Clean Air Nonroad Diesel - Tier 4 Rule (69 FR
  38957, June 29, 2004). In 2004, EPA adopted a
  comprehensive national program to reduce emissions
  from future nonroad diesel engines by integrating
  engine and fuel controls as a system to gain the
  greatest emission reductions. EPA estimates that in
  2030, this program will reduce annual emissions of
  NOX by about 740,000 tons.
4. Marine-Related NOX Reductions from 1999 to 2003.
  EPA completed three rulemakings with respect to the
  diesel marine sector that will reduce NOX emissions.
  These rules are now in effect and being phased-in.
  In 1999 (64 FR 73299, December 29, 1999), EPA
  promulgated NOX requirements for diesel engines
  used in commercial boats (large inland and near-
  shore boats) and commercial vessels (ocean-going
  vessels). EPA estimates that these reduced emissions
  from these vessels by about 30%. In 2002 (67 FR
  68241, November 8, 2002), EPA promulgated rules
  reducing NOX emissions from diesel engines used in
  recreational marine vessels by 25%. In 2003 (68 FR
  9746, February 28, 2003), EPA promulgated another
  rule further reducing NOX from diesel engines used in
  commercial vessels by about 20%. EPA projects that
  on a nationwide basis, these four programs will reduce
  marine-related NOX by more than 1 million tons in
  2030.
5. Locomotive and Marine Diesel Rule (73 FR 25098,
  May 6, 2008). In March 2008, EPA adopted standards
  that will reduce NOX emissions from locomotives
  and marine diesel engines. The near-term emission
  standards for newly-built engines phased in starting
  in 2009. The long-term standards begin to take effect
  in 2015 for locomotives and in 2014 for marine diesel
  engines. EPA estimates NOX emissions reductions
  of 80% from engines meeting these standards. EPA
  projects that in 2030, about 420,000 tons of NOX will
  be reduced from the locomotive engines, and 375,000
  tons of NOX will be reduced from commercial and
  recreational marine engines.
6. Non-road Spark-Ignition Engines (73 FR 59034,
  October 8, 2008). In 2002, EPA promulgated emissions
  standards for large spark-ignition engines. These took
  effect in 2004 for Tier 1  standards and in 2007 for Tier
  2 standards. EPA promulgated emissions standards
  for small spark-ignition engines in 2008. EPA projects
  that, when fully implemented, the new standards will
  result in a 35% reduction in HC+NOX emissions from
  new engines' exhaust, reduce evaporative emissions by
  45%, and that together these programs will reduce NOX
  by more than 585,000 tons in 2030.
7. EPA's Coordinated Strategy for Control of Emissions
  from Ocean-Going Vessels (www.epa.gov/otaq/
  oceanvessels.htm). EPA's coordinated strategy to
22 The information in Appendix F was provided to the Integrated Nitrogen Committee by Mazrgaret Zawacki of the U.S. EPA Office of Transportation
  and Air Quality.

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 control emissions from ocean-going vessels consists
 of actions at the national and international levels.
 On December 22, 2009, EPA finalized emissions
 standards for ocean-going vessels which take effect
 in 2011. In addition to this rule the U.S. Government
 has also amended MARPOL Annex VI to designate
 U.S. coasts as an Emission Control Area (EGA) in
 which all vessels, regardless of flag, will be required
 to meet the most stringent engine and marine fuel
 sulfur requirements in Annex VI. New engine emission
 and fuel sulfur limits contained in the amendments to
 Annex VI are also applicable to all vessels  regardless
 of flag and are implemented in the U.S. through the Act
 to Prevent Pollution from Ships (APPS). EPA projects
 that when fully implemented, the coordinated strategy
 will reduce NOX emissions from ocean-going vessels
 by 80% and that in 2030, the coordinated strategy
 is expected to yield a reduction in NOX of about 1.2
 million tons.
. EPA's Voluntary Clean Diesel Programs. EPA has
 created a number of programs designed to reduce
 emissions (including both PM and NOX) from the diesel
 fleet. In conjunction with state and local governments,
  public interest groups, and industry partners, EPA has
  established a goal of reducing emissions from the over
  11 million diesel engines in the existing fleet by 2014.
  Looking at these engines, EPA determined there were
  general sectors that provided the best opportunity to
  obtain significant reductions and created programs for
  Clean Agriculture, Clean Construction, Clean Ports,
  Clean School Bus, and SmartWay Transport.
9. Section 177 of the Clean Air Act allows states outside
  of California to adopt California emissions standards,
  once EPA has granted such a waiver. As a result,
  several northeastern states have adopted California
  standards. Maryland adopted its California LEV II
  NOX standards as part of its Low Emission Vehicle
  Program (COMAR 26.11.34, effective December 17,
  2007). These standards take effect with the 2011 model
  year. Maryland submitted that program to EPA as a
  SIP revision. Pennsylvania adopted California LEV II
  NOX standards as part of its Clean Vehicles Program
  (codified at Pa. Code Chapters 121 and 126, effective
  December 9, 2006). Pennsylvania's program began
  with model year 2008 vehicles. Pennsylvania submitted
  this program as a SIP revision.

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Nitrogen contamination ofgroundwater
   In addition to environmental concerns about
N-nutrient loading to freshwaters from a groundwater
pathway, there are also potential human health impacts
from elevated levels of N in groundwater, especially
from NO3. It has been long established that excess NO3
in drinking water supplies can cause blue baby syndrome
(methemoglobinemia) (Knobeloch and Proctor 2001;
Ward et al. 2005, 2006), the indicator of which is MetHb.
To protect public health from effects  of NO3 in drinking
water, EPA has established a maximum contaminant level
goal (MCLG) that considers a lifetime exposure plus a
margin of safety. For NO3 in drinking water, the MCLG
is 10 mg/L. Nitrite-N also has an established MCLG of
1 mg/L, and the combined NO3 and NO2 MCLG set by
EPA is 10 mg/L. These same values are used to regulate
NO3 and NO2  as maximum contaminant levels (MCL),
which are the highest levels of contaminants allowed in
drinking water (40 CFR § 141.62).
   The drinking water standard is commonly exceeded
in streams and rivers of the U.S., particularly in the
agricultural Midwestern U.S. For example, there were  13
episodes over a 25- year period of formal warnings by
authorities to local citizens in Columbus, Ohio, about not
drinking tap water because nitrate-nitrogen was higher
than 10 mg-N/L (Mitsch et al., 2008). These episodes
lasted from one to several weeks each. The pattern is
generally for high concentrations of nitrate-nitrogen
in Midwestern rivers from February through June or
July. In one pattern, averaged over 7 years with weekly
river sampling, nitrate-nitrogen in a central Ohio river
peaked with an average of 7 mg-N/L  in June after which
concentrations decrease to 1-2 mg-N/L for the rest of the
summer and fall. The year-to-year variability was high
for that month as well (Mitsch et al. 2005) as these spring
"high-nitrate" floods do not occur every year. The nitrate
"pulses" generally are part of flood events after fertilizer
has been applied to fields in the watershed.
   Public policy by water supply agencies is to treat
high concentrations of nitrate-nitrogen in drinking water
supplies as a real public health threat. Recent studies
have brought the concern of high nitrate-nitrogen in
drinking water into dispute (Ward et al., 2005, 2006).
While there is  a definite link between excessive nitrate
in drinking water and methemoglobinemia, there is also
a need to better understand the interaction of the range
of environmental factors (e.g., cofactors such as diarrhea
and respiratory diseases reportedly increase MetHb
levels) that promote methemoglobinemia. This will
help identify the environmental conditions under which
exposure to nitrate in drinking water poses a risk of
methemoglobinemia.
   According to the USGS (Barber, 2009) Summary of
Estimated Water Use in the U.S. in 2005, total water
withdrawals in the U.S., excluding thermoelectric power
usage, were 210 billion gallons per day, of which 44,200
million gallons per day (MOD) were for public water
supply. About two-thirds of that supply is provided by
surface water, the  rest is from wells and about 58%
(25,600 MOD) of public water supply goes towards
domestic use, including drinking water. Private wells
(Figure G-l) that are not part of public water supply
systems are estimated to provide an additional 3,830
MOD, providing domestic water for 42.9 million people
(14% of the U.S. population in 2005).
   Groundwater N in forested and low intensity (<10%)
agriculture or urban land use areas is estimated to be
fairly low, having  75th percentile concentrations of 0.5
and 1.1 mg/L in two USGS studies (Nolan and Hitt,
2002). They consequently concluded that a "reasonable"
background concentration, as NO3-N, would be 1.1 mg/L,
which would include effects in more sensitive aquifers
with nominal loading for urban or agricultural sources.
   Nitrate can enter groundwater from a variety of
sources, including all of those described in this report,
but fertilizer and animal waste in rural, agricultural areas
are especially prominent sources (Nolan and Ruddy,
1996). Other sources include septic systems, more
important in densely-developed and unsewered urban
areas, and atmospheric deposition. Vulnerability to
elevated NO3 levels is also variable, but an assessment
and model by Nolan and Hitt (2006) predicted that"...
areas with high N  application, high water input, well-
drained soils, fractured rocks or those with high effective
porosity,  and lack  of attenuation processes..." are
especially vulnerable (r2 = 0.801).
   As illustrated in Figure G-2, surveys confirm that
NO3 in groundwater is elevated in many areas of the
U.S., well above the 1.1 mg/L background upper bound
described above (Figure G-2). The background colors
on the map in Figure G-2 are indicative of different types
of aquifers. In a 1992-1995 survey (Nolan and Stoner,
2000), shallow groundwater underlying agricultural areas
was found to be most severely impacted by elevated
NO3-N levels (median concentration of 3.4 mg/L).
Urban shallow aquifers were less impacted (median
concentration of 1.6 mg NO3-N/L) and deeper, major

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aquifers, in general, had a median NO3-N concentration
of 0.48 mg/L. However, NO3-N concentrations did
exceed the 10 mg/L MCL threshold set by EPA for
drinking water in more than 15% of the groundwater
samples in the survey from drinking water aquifers.
   In the most recent survey of domestic well water
quality (DeSimone et al., 2009), USGS found
concentrations of NO3-N greater than 10 mg/L in 4.4%
of the wells sampled. Concentrations exceeding the
nitrate MCL were most frequently encountered in certain
basins of the Southwest and California, west-central
glacial aquifers in the Upper Midwest, and coastal plain
aquifers and Piedmont crystalline rock aquifers in central
Appalachia. Lowest concentrations were found in the
coastal plain aquifers of the Southeast. In general, higher
NO3-N concentrations were found near agricultural
lands. In an additional analysis of shallow groundwater
wells in agricultural areas, separate from the national
survey, nearly 25% of the sampled wells exceeded the 10
mg/L MCI for NO3-N. DeSimone et al. (2009) suggested
that redox could be a defining factor in some cases, and
perhaps was the reason for low NO3-N concentrations in
the Southeast in soils that promote denitrification, as well
as higher NO3-N levels in other areas where aquifers
were better oxygenated.

Ammonia toxicity in freshwater systems
   The EPA and states have long regulated ammonia
(NH3) in the environment, not because of its nutrient
contribution to cultural eutrophication, but because of
its toxicity to freshwater aquatic life (U.S. EPA, 1986).
The un-ionized ammonia molecule has been identified
as the primary toxic form, rather than the ammonium
ion (NH4+), and research has further demonstrated
the relationship between pH, temperature, and NH3
partitioning from the total ammonia pool in freshwaters
(U.S. EPA, 1999). Because of the relationship to
temperature in particular, and the variable sensitivity of
species and life stages of aquatic organisms, state water
quality standards in application generally consider cold
and warm water conditions, as well as acute and chronic
exposures to life stages of sensitive organisms, plus a
margin of safety to derive criteria.
   While water quality criteria were initially set for
concentrations of NH3, as criteria development skills
and understanding improved, it made sense to develop
criteria for total ammonia concentration for specific
water quality conditions, e.g., cold or warm (salmonids
present or absent) with consideration of ambient pH
factors as appropriate, for protection of the most
sensitive species likely to be present (early life stages
present or absent), plus a margin of safety. This was
because of evidence that the NH4+ fraction may also
be contributing to toxicity. Criteria could be presented
as formulas in adopted state criteria to calculate total
ammonia thresholds based on prevailing pH and
temperature conditions and organisms present/absent,
as appropriate.
   Based on this research and analysis, EPA currently
recommends adoption of ammonia criteria as criterion
continuous concentration (CCC) and criterion maximum
concentration (CMC) as described in Box G-l (U.S.
EPA, 1999).
                                                                                EXPLANATION
                                                                              PopuUrion (199 5) supplied by
                                                                               domestic well!, in percent of
                                                                               total county population
                                                                              IB >40snd<60
                                                                              S3 >20and<40
                                                                              CD <20
                 In many parts of the United States, domestic wells supply drinking water for large percentages ol the population.
          Nationwide, more than (3 million people rely on domestic wells. Data shown are from Solley and others (1998)
Figure G-1: U.S. Population (1995) supplied by domestic drinking water wells
Source: DeSimone et al., 2009.

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                                             vj                       \)
                                           EXPLANATION
                                    • =-10
                                                            3 il
                   Concentrations of nitrate were greater than the U.S. Environmental Protection Agency Maximum
           Contaminant Level of 10 milligrams per liter (mg/LI as N in 4.4 percent of the wells. Elevated concentrations of
           nitrata occurred throughout the United Slates, but least commonly in the Southeast Concentrations greater than
           1 mg'L as N. which is usually indicative o1 human activities in many areas (Nolan and Hitt, 2003), were found in
           about 40 percent of wells located throughout the sampled areas
Figure G-2: Nitrate concentrations in U.S. domestic drinking water wells
(Background colors on the map are indicative of different aquifer types)
Source: DeSimone et al., 2009
   Recent research on the sensitivity of freshwater
unionid mussels (Family, Unionidae), and the rare and
endangered status of several unionid species, has led
the EPA to issue a draft update to the ammonia criteria
guidance (U.S. EPA, 2009b) that would supersede
the current 1999 guidance. Mussels have been found
to be more sensitive to ammonia toxicity than the
most sensitive species used to derive the 1999  criteria
(Augspurger et al., 2003). Augspurger et al. (2003) found
that the CMC with unionids considered would range
from 1.75 to 2.50 mg total ammonia-N/L, 60% lower
than the current method calculation of 5.62 mg total
ammonia-N/L, for example. In the draft 2009 update,
EPA proposed a two-tiered process for ammonia CMC
and CCC development for waters with and without
sensitive unionid species.
   With this new sensitivity identified, ecosystem
imbalances due to eutrophication and the presence and
die off of invasive species  may result in toxic levels of
ammonia for mussels in freshwater systems, in addition
to the conventional sewage and agricultural sources. For
example, Cooper et al., (2005) suggested that die offs of
invasive Asian clam (Corbicula fluminea), common in
most southeastern U.S. waters, could produce enough
sediment pore water ammonia during decay to be lethal
to sensitive unionid mussels. Further, combination of
ammonia with other toxic substances may compound
toxic effects, as found by Wang et al. (2007a,b) for
ammonia and copper. Their research suggests that the
1999 criteria for total ammonia might not be protective
of sensitive mussel species.

Impacts ofNr on freshwater ecosystems
  Reactive nitrogen (Nr), including reduced
(ammonium, organic N compounds) and oxidized
(nitrate, nitrite) forms, play central roles in modulating
and controlling (limiting) primary and secondary
production and species composition in freshwater
ecosystems.  These include lakes, reservoirs, streams,
rivers, and wetlands (Goldman, 1981; Paerl, 1982; Elser
et al., 1990, 2007; Wetzel, 2001).  While phosphorus
has been considered the primary limiting nutrient in
freshwater ecosystems (c.f.  Schindler, 1971; Schindler
et al., 2008), there are numerous examples where Nr
plays either a primary or secondary (i.e., co-limiting)
role as a limiting nutrient (Paerl,  1982; North et al.,
2007; Wurtsbaugh et al., 1997; Lewis and Wurtsbaugh,
2008). In particular, oligotrophic, alpine, tropical and
subtropical, and other lakes having small watersheds

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                      Box G-1: The National Criterion for Ammonia in Fresh Water
  The available data for ammonia, evaluated using the procedures described in the "Guidelines for Deriving
  Numerical National Water Quality Criteria for the Protection of Aquatic Organisms and Their Uses," indicate
  that, except possibly where an unusually sensitive species is important at a site, freshwater aquatic life should be
  protected if both of the following conditions are satisfied for the temperature (T) and pH of the waterbody:
  1. The one-hour average concentration of total ammonia nitrogen (in mgN/L does not exceed, more than once every
    three years on the average, the CMC (acute criterion) calculated using the following equations. Where salmonid
    fish are present:
    CMC =
                 0.275
            39.0
               + 10
                   7.204-/>//
       1+](K//-7.204
    Or where salmonid fish are not present:
                 0.411            58.4
     CMC =
              1 + 10
                   7.204-pH
                                      -7.204
  2A. The 30-day average concentration of total ammonia nitrogen (in mg N/L) does not   exceed, more than once
     every three years on the average, the CCC (chronic criterion) calculated using the following equations.
     When fish early life stages are present:

                   0.0577          2.487
      ccc =
                1 + 10
7.688-p//
          1 + 10
                                      ptf-7.688
                           M//X2.85,  1.45-10
                                                                      (0.28-25-7") •
     When fish early life stages are absent:
                   0.0577         2.487
      ccc =
                         •1.45-10
                                                        Q.Q2«-(25-MAX(T,l))
              v                 1 + 10*"    «j

  2B. In addition, the highest 4-day average within the 30-day period should not exceed 2.5 times the CCC.
Source: U.S. EPA, 1999.
relative to the lake surface/volume, and lakes
experiencing incipient stages of eutrophication, tend to be
N-limited (Wetzel, 2001; Lewis and Wurtsbaugh, 2008).
N limitation was illustrated for Lake Tahoe (in California
and Nevada) which was highly sensitive to N enrichment
during its early stages of eutrophication (Goldman.
1981; 1988). As the lake accumulated anthropogenic
N inputs  from both land-based runoff and atmospheric
deposition within the Tahoe Basin, it began exhibiting
symptoms of accelerating eutrophication, including
noticeable "greening" of its formerly transparent near-
shore waters and excessive epiphytic growth and fouling
on its rocky  bottom.  Continued excessive N loading in
the 1960s through 1980s has led to accelerating rates
of algal primary production and a tendency to shift to
more P limited conditions due to excessive N, relative
to P, loading (Goldman, 1988). This greater than 30-year
                                  progression to more eutrophic, and less desirable (from
                                  ecological, trophic and economic perspectives—i.e..
                                  tourism, water use) conditions has largely been spurred
                                  on by excessive N loading. Recent measures taken to
                                  reduce N inputs have been successful in reducing the
                                  lake's rate of eutrophication (Goldman, 2002).  Similarly.
                                  Lake Erie, which has experienced P-driven nuisance algal
                                  blooms starting in the 1950s, is now facing excessive N
                                  loading. This is largely a result of P input restrictions.
                                  which have been enacted since the 1970's, accompanied
                                  by a lack of control on ever-increasing N loads. This
                                  shift in nutrient loading (increasing N:P) has led to a
                                  resurgence of toxic cyanobacterial blooms dominated
                                  by the non-N2 fixing genus Microcystis, an indicator of
                                  excessive N loading (North et al., 2007).
                                     Numerous lakes, reservoirs, rivers, estuaries (e.g., the
                                  Gulf of Mexico), and fjords worldwide exhibit N and

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P co-limitation, either simultaneously or in seasonally-
shifting patterns (Dodds et al, 1989; Elser et al, 1990,
2007; Elmgren and Larsson, 2001; Forbes et al., 2008;
Scott et al., 2008; Wetzel, 2001; North et al., 2007; Xu
et al., 2010). For example, many reservoirs in Texas
exhibit seasonal N limitation in the river-reservoir
transition zone, regardless of their trophic status (Scott
et al., 2009). Under these  circumstances, N inputs tend
to determine the spatial and temporal extent of summer
nuisance algal blooms, a key symptom of degrading
water quality (Dodds et al., 1989; Paerl, 2009; Xu et al.,
2010). N inputs, including those from increasing levels
of atmospheric deposition, impact nutrient stoichiometry,
with cascading effects on nutrient limitation, productivity,
and lake nutrient cycling characteristics (Elser et al.,
2009). Therefore, the inputs of N play a critical role in the
overall trophic response, trophic state, and water quality
conditions of affected freshwater ecosystems.
  In Florida lakes, algae  are often limited by the
availability of Nr (Kratzer and Brezonik, 1981). The
most well-studied example is Lake Okeechobee, the
largest lake in the Southeastern U.S., and a system that
periodically displays large blooms of noxious blue-green
algae. This lake has high availability of reactive P, and
changes in the availability of Nr control the wax and
wane of algae. In the 1980s and 1990s, blooms of algae
were predominantly caused by cyanobacterial nitrogen
(N2) fixer Anabaena. However, the most widespread
recent bloom, which covered almost the entire lake
surface in summer 2006, was caused by Microcystis, a
non-N2- fixing cyanobacterium that depends on dissolved
inorganic N (DIN: ammonium, nitrate, nitrite) and
possibly organic N for its growth. This alga is the most
common producer of toxins in Florida lakes, and it has
the ability to "luxury consume" P from lake sediments
and then rise through the water column, increasing
its biomass to a level that largely is controlled by the
amount of DIN. Because Lake Okeechobee's sediments
contain massive quantities of reactive P (Havens et al.,
2007), successful control  of Microcystis blooms will
require reduction in both P and N inputs to this lake.
  In addition to the importance total N loads play
in determining water quality status and trends, the
supply rates and ratios of various Nr forms play an
important role in structuring microalgal and macrophyte
communities  mediating freshwater primary production
(Paerl, 1988;  McCarthy et al., 2007, 2009; Lin et al.,
2008). For example, the ratio of ammonium to oxidized
N was related to the proportion of cyanobacteria
composing the total phytoplankton community of
Lake Okeechobee (McCarthy et al., 2009). Non-N2-
fixing cyanobacteria, such as Microcystis, are superior
competitors for reduced N (Blomqvist et  al., 1994),
but even N2-fixing  cyanobacteria will preferentially
assimilate ammonium if it is available (Ferber et al.,
2004). Ammonium is the  initial N form produced by
recycling processes (via invertebrate excretion and
bacterial mineralization), but standing concentrations
often remain very low because they are assimilated
rapidly. Ammonium and other reduced N forms, such
as dissolved free amino acids, are more available than
oxidized N forms (nitrate and nitrite) to bacteria (Vallino
et al., 1996) and cyanobacteria because less energy is
required to incorporate reduced N into biomass than for
oxidized forms (Syrett, 1981; Gardner et al., 2004; Flores
and Herrero, 2005).
   Lastly, it should be pointed out that both freshwater
and marine systems do not respond to nutrient inputs
in isolation. These systems are hydrologically and
biogeochemically connected and coupled, functioning
as a freshwater to marine continuum (Paerl, 2009).
Nutrient limitation may shift along the continuum, and
eutrophication and other symptoms of N and P over-
enrichment, including harmful algal blooms, hypoxia,
loss of biodiversity, and food web alterations impact
water quality, habitat condition, use, and  sustainability
of downstream waters. Therefore, excessive N loading
in upstream freshwater ecosystems, ranging from the
headwaters of pristine alpine streams to lowland lakes,
reservoirs, and rivers can adversely affect downstream
estuarine and coastal marine waters (Conley et al., 2009;
Paerl, 2009). Examples of such continuum-scale impacts
include such prominent systems as Chesapeake Bay,
Albemarle-Pamlico Sound, Florida Bay, Mississippi
River plume (Gulf of Mexico), Baltic Sea, and Coastal
North Sea (Elmgren and Larsson, 2001; Boesch et al.,
2001; Paerl, 2009).

Impact of nitrogen on wetlands
   In this section, the possible impact of reactive nitrogen
on wetlands is discussed. In Chapter 5 considerable
attention is devoted to the subject of wetlands serving
as effective nitrogen sinks. There are about 110 million
ha of wetlands in the U.S., with more than half of
those in the  state of Alaska (Mitsch et al., 2009). Of
those wetlands, roughly 97% are inland (and mostly
freshwater) and 3% are estuarine (and mostly saline). Of
the total wetlands, approximately half (166 million acres
or 55 million ha) are  peatlands, which, by their nature  as
low-nutrient systems, are most susceptible to nitrogen
loadings, either from the atmosphere or from rivers and
streams. More than any other ecosystem, wetlands are
central to the cycling of nitrogen because they have both
aerobic and anaerobic conditions that allow for a wide
variety of important nitrogen processes, not the least of
which is denitrification.
   Wetlands are similar to lakes and streams and any
other ecosystem in that their productivity  is limited by
nutrient availability. But with wetlands the hydrology
limits or enhances productivity as well (Mitsch and
Gosselink, 2007). The addition of excessive nutrients
to wetlands, while often done purposefully when the
wetlands are so-called treatment wetlands (Kadlec and

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Wallace, 2009), can cause vegetation shifts and decreases
in plant diversity. Verhoeven et al. (2006) suggested 4.5
g-N nr2 yr1 as critical loading rate of nitrogen, generally
from atmospheric sources, for peat-dominated wetlands.
Morris (1991) had suggested that bogs and fens generally
had loading rates of 1 to 6 g-N nr2 yr1 respectively.
Thus Verhoeven et al. (2006) were suggesting that
wetlands should not be loaded beyond what is currently
occurring in fen peatlands. These limitations do not
apply for most mineral soil wetlands, particularly those
connected to streams and rivers. Most freshwater and
tidal marshes have nitrogen loading rates closer to 60
g-N nr2 yr1 and they maintain a reasonably  high and
sustainable productivity. Using a sustainable rate of
nitrogen retention as a measure, Mitsch and Jergensen
(2004) suggest a range of nitrogen retention rate of 10-20
g-N nr2 yr1 for wetlands to maintain their biodiversity
while being nitrogen sinks at the same time. Overall, this
is a fruitful direction for wetland research to  determine
the assimilative capacity of wetlands for nutrients,
including nitrogen, while not surpassing a limit that will
dramatically change the wetland's structure and function.

Impacts of Nr on coastal systems
  Mitsch et al. (2001) suggest that streams and rivers
themselves are not always as much affected by nutrient
loading as are lakes, wetlands, coastal areas,  and other
lentic bodies of water. However, in most cases, these
nutrient-enriched waterways flow to the sea,  with
eutrophication of coastal waters the unfortunate result.
This problem now occurs regularly throughout the world
(World Resources Institute, 2008), in locations such as
the Gulf of Mexico (Rabalais et al., 1996), the Baltic Sea
(Larson et al., 1985), and the Black Sea (Tolmazin, 1985).
  During the past century, following large-scale use of
synthetic N fertilizers in agriculture, rapid expansion
of industrial and transportation-related fossil fuel
combustion and coastal urbanization, humans have
significantly altered the balance between "new" N inputs
and N losses in the marine environment (Codispoti et al.,
2001; Galloway and Cowling, 2002). During this time
frame, terrestrial discharge and atmospheric N emissions
have increased 10-fold (Howarth et al., 1996; Holland
et al., 1999). This number keeps growing as human
development continues to expand in coastal watersheds
(Vitousek et al., 1997a,b).
  Researchers have long recognized this growing
imbalance, especially in estuarine and coastal waters
where anthropogenically-derived N over-enrichment
has fueled accelerated primary production, or "cultural"
eutrophication (Vollenweideretal., 1992; Nixon, 1995).
Eutrophication is a condition where nutrient-enhanced
primary production exceeds the ability of higher ranked
consumers and organic matter-degrading microbes to
consume and process it. D'Elia (1987) characterized
this condition as "too much of good thing" or over-
fertilization of N-limited marine ecosystems with "new"
N, the bulk of it being anthropogenic (Howarth et al.,
1996; Vitousek et al., 1997a,b; Galloway and Cowling,
2002). Symptoms of N-driven eutrophication vary: from
subtle increases in plant production to changes in primary
producer community composition; to rapidly accelerating
algal growth, visible discoloration or blooms, losses in
water clarity, increased consumption of oxygen, dissolved
oxygen depletion (hypoxia), which is stressful to resident
fauna and flora; to, in the case of total dissolved oxygen
depletion (anoxia), elimination of habitats (Paerl,
1988, 1997; Diaz and Rosenberg, 1995; Rabalais and
Turner, 2001). Other effects include submerged aquatic
vegetation (SAV) losses, possible impacts on tidal
wetland health, and disruption of estuarine food chain
dynamics that may favor an imbalance towards lower
trophic levels (e.g., jellyfish).
   Anthropogenic or cultural eutrophication has
been closely linked to population densities in coastal
watersheds (Peierls et al., 1991; Nixon, 1995; Vitousek
et al., 1997a,b). Primary sources of N enrichment
include urban and agricultural land uses as well as
wastewater treatment plants, many of which have not
been designed to remove N. A significant, and in many
instances increasing, proportion of "new" N input can
also be attributed to remote sources residing in airsheds.
Delivery routes can also be complex, especially when
via subsurface aquifers outside the immediate watershed,
which can confound source definition and create long
delays in delivery and management response (Paerl, 1997;
Jaworski et al., 1997; Galloway and Cowling, 2002; Paerl
et al., 2002).
   The availability of N controls primary production in
much of the world's estuarine, near-shore coastal, and
open-ocean waters (Dugdale, 1967; Ryther and Dunstan,
1971; Nixon, 1995; Paerl, 1997; Boesch et al., 2001).
As previously discussed, nitrogen can also play a role
as either a primary or secondary limiting  nutrient in
freshwater environments, especially large lakes (e.g.,
Lake Tahoe, Lake Superior). As such, the fertility of
these waters is often closely controlled by N inputs,
which are provided either internally by regeneration of
pre-existing N and biologically-fixed atmospheric N2,  or
supplied externally (i.e. "new" N) as combined N sources
delivered via surface runoff, sub-surface groundwater,  or
atmospheric deposition.
   The extent to which accelerated N loading promotes
eutrophication and its symptoms varies greatly among
marine ecosystems. Receiving waters exhibit variable
sensitivities to N and other nutrient [phosphorus (P), iron
(Fe), and silica (Si)] loads that are controlled by their size,
hydrologic properties (e.g., flushing rates and residence
times), morphologies (depth, volume), vertical mixing
characteristics, geographic and climatic regimes and
conditions. In addition, the magnitude and distribution of
N in relation to other nutrient loads can vary substantially.
In waters receiving very high N loads relative to

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requirements for sustaining primary and secondary
production, other nutrient limitations may develop. This
appears to be the case in coastal waters downstream of
rivers draining agricultural regions that are enriched in N.
   On the ecosystem level, estuarine and coastal waters
exhibit individualistic responses to N loads over seasonal
and longer (multi-annual, decadal) time scales. The
degree to which these systems are exposed to freshwater
discharge, tidal exchange, and vertical mixing is critical
for determining how they respond to specific N loads
(Vollenweideretal., 1992; Nixon, 1995; Cloern, 1999,
2001; Valdes-Weaver et al., 2006; Paerl et al., 2007).
Another variable is the manner in which N loading takes
place, which may range from acute pulsed events such
as storms and associated flooding, to longer-term gradual
(chronic) increases in N loading associated with more
predictive seasonal, annual and inter-annual hydrologic
cycles. There are striking contrasts in ecosystem response
to N inputs that reflect a range in physical (hydrodynamic,
optical) and  climatic conditions (Cloern, 1999, 2001).
Examples include contrasts between strong tidally-
driven estuarine systems, such as Delaware Bay and San
Francisco Bay, and non-tidal, lagoonal systems, such as
North Carolina's Pamlico Sound and Texas's Luguna
Madre, or semi enclosed coastal systems, such as Florida
Bay and the  Long Island Sound (Bricker et al., 1999;
Valdes-Weaver et al., 2006; Paerl et al., 2007).
   Externally-supplied N comes in various forms,
including organic N and inorganic reduced (NH3 and
NH4+ ion) and oxidized (NO3~) N, all of which are
potentially available to support new production and
eutrophication. Laboratory experiments on phytoplankton
isolates and bioassays with natural phytoplankton
communities have indicated that these contrasting
forms may be differentially and preferentially utilized,
indicating that, depending on composition of the affected
phytoplankton community, some forms are more reactive
than others (Collos, 1989; Stolte etal.,  1994; Riegman,
1998). Phytoplankton community composition can also
be altered by varying proportions and supply rates of
different forms of N (Dortch, 1990; Stolte et al., 1994;
Harrington,  1999; Pinckney  et al., 1999; Piehler et al.,
2002). Monitoring and research on dissolved organic N
inputs and their effects should be conducted in receiving
streams, rivers, lakes, estuarine, and coastal waters, since
there is evidence that these compounds can be utilized by
phytoplankton, including harmful bloom species (Paerl,
1988; Antia  et al., 1991; Carlsson and Graneli, 1998;
Gilbert et al., 2006). In addition, specific N compounds
may interact with light availability, hydrodynamics and
other nutrients, most notably P, Si, Fe, and trace metals,
to influence phytoplankton community  growth rates and
composition (Harrison and Turpin, 1982; Smith, 1990;
Dortch and Whitledge, 1992).
   Over the past 25 years, there has been a growing
recognition of cultural eutrophication as a serious
problem in coastal estuaries (NRC, 2000). Globally,
Selman et al. (2008) have reported "Of the 415 areas
around the world identified as experiencing some form
of eutrophication, 169 are hypoxic and only 13 systems
are classified as 'systems in recovery."Comprehensive
surveys of U.S. estuaries have been conducted by the
National Oceanic and Atmospheric Administration
(NOAA) as part of the National Estuarine Eutrophication
Assessments (NEEA) in 1999 and 2004 (Bricker et al.,
1999, 2007). The most recent report, released in 2007
(Bricker et al., 2007) focused on nutrient enrichment
and its manifestations in the estuarine environment and
relies on participation and interviews of local experts
to provide data for the assessment. Among the key
findings for nearly 100 assessed U.S. estuaries were that
eutrophication is a widespread problem, with the majority
of assessed estuaries showing signs of eutrophication—
65% of the assessed systems, representing 78% of
assessed estuarine area,  had moderate to high overall
eutrophic conditions. The most common symptoms of
eutrophication were high spatial coverage and frequency
of elevated chlorophyll a (phytoplankton)—50% of the
assessed estuaries, representing 72% of assessed area, had
a high chlorophyll a rating.
  Further field evaluations by EPA and state and
university collaborators under the National Coastal
Assessment (NCA) used probabilistic monitoring
techniques. The NCA National Coastal Condition Reports
(NCCR) (U.S. EPA, 200la, 2004, 2006b) are more closely
related to nutrient enrichment assessments, especially for
manifestations of nutrient enrichment such as hypoxia,
nuisance algal blooms, and general habitat degradation.
The last comprehensive national NCCR was published
in 2004 (U.S. EPA, 2004) with a more recent assessment
focused on 28 National Estuary Program estuaries
published  in 2007 (U.S. EPA, 2006). The 2004 NCCR
included an overall rating of "fair" for estuaries, including
the Great Lakes, based on evaluation of more than 2,000
sites. The water quality index, which incorporates nutrient
effects primarily as chlorophyll-a and dissolved oxygen
impacts, was also rated "fair" nationally. Forty percent
of the sites were rated "good" for overall water quality,
while 11% were "poor"  and 49% "fair."

Attainment of water quality management goals
and standards for coastal systems
  Estuarine systems, where bio-available Nr is
more likely to be the limiting nutrient, are most often
susceptible to Nr enrichment (Paerl, 1997; Boesch et al.,
2001). Defining single-number criteria for nutrients or
related indicators representative of undesirable levels of
productivity (e.g., chlorophyll-a)  is difficult, even using
the ecoregional approach recommended by EPA. State
managers  more often use the formal TMDL process or
collaborative estuarine management plans to set site- or
estuary-specific N management targets to meet existing,
related water quality criteria (e.g., dissolved O2  or

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chlorophyll a). Some of the more prominent efforts and
targets for nitrogen control are summarized in Table G-l.
   These targets all exceed nitrogen load reduction goals
that the Committee has found to be readily achievable
using existing technology and management authority
(i.e., less than 25% from specific source categories).
Some sources of nitrogen loading to the estuaries in
Table G-l pose greater management challenges and
the expectation is that reductions in Nr loadings to
estuaries would cumulatively be less than 25%. This
suggests that efforts will be needed to enable even
greater nitrogen load reductions in the future. Many of
the management actions the Committee has proposed in
this report would require substantive changes in national
programs, regulatory authority,  management technologies
and societal demands to be accomplished. This is a
nutrient management concern that state managers are
well aware of as they develop TMDLs and management
plans that range  above attainment potential, not only
for Nr but more frequently for other pollutants that are
predominately nonpoint source  and stormwater loaded
(including atmospheric source contributions).
                The Chesapeake Bay Program, for example, is
             a model for Nr and P management in many ways.
             Considerable resources were committed, and many
             BMPs were implemented. Yet despite regional efforts
             and commitments from all watershed states, and more
             funding than any other estuary program is likely
             to see, management targets have not been met, and
             recent data (2007) reveal the occurrence of a severe
             hypoxic episode. Concerns over the slow progress in
             restoring the Chesapeake Bay led to the issuance of an
             Executive Order on May 15, 2009, establishing a Federal
             Leadership Committee led by the EPA to develop and
             implement a plan to restore the Bay in collaboration with
             state agencies (Federal Register 74(93): 23097-23104).
             Similarly, the adoption of the Long Island Sound TMDL,
             which was driven by the presence of reactive nitrogen
             (see Box G-2), sets an implementation plan that could
             attain Connecticut and New York dissolved oxygen
             criteria, but only if "alternative technologies" such as
             mechanical aeration of the Sound or biological harvesting
             of nutrients, are used.
                      Table G-1: Estuaries with nitrogen management plans or
                         TMDLs and percent nitrogen load reduction targets
      Casco Bay, Maine
                                               trogen Load
                                              .duction Target
           45%
                                   TMDL or Plan
    Plan
      Chesapeake Bay
          >40%
    Plan
      Northern Gulf of Mexico
      Mississippi Plume Region
           45%
    Plan
      Long Island Sound
 60% for CT & NY sources
   TMDL
      Neuse River Estuary, NC
           30%
   TMDL
      Tampa Bay, FL
Maintain TN (total nitrogen)
 load at 1992-1994 levels
TMDL & Plan

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        Box G-2: Long Island Sound Total Maximum Daily Load: Focus on Reactive Nitrogen

A TMDL sets a goal for reducing the load of a specific pollutant that is causing impairment to a waterbody. In
the case of Long Island Sound, the impairment constitutes low concentrations of dissolved O2 that violate both
Connecticut's and New York's water quality standards. Nitrogen has been identified as the pollutant that causes
substandard levels of dissolved oxygen in Long Island Sound and, accordingly, Connecticut's and New York's
environmental agencies have developed a TMDL that assigns nitrogen reductions from both point sources (the
wasteload allocation or WLA) and nonpoint sources (the load allocation or LA) in their respective states to meet the
established 58.5% reduction of anthropogenic sources.
The Long Island Sound TMDL is set at 23,966 tons of N/year, which represents a 23,834 ton/year reduction from
the total baseline  (anthropogenic + natural sources considered) of 47,788 tons/year from Connecticut and New
York only. Most of that N load comes from point sources - POTWs (publicly owned treatment works) and CSOs
(combined sewer overflows) - accounting for 38,899 tons/yr of the total N load from the two states, or 81% of the
load. For that reason, the  focus has been on managing point sources, although attainment of water quality  standards
will require more widespread  reductions from atmospheric deposition, stormwater, and nonpoint sources, and from
other watershed states north of Connecticut.
Connecticut and New York have some flexibility in the apportionment of those reductions between the WLA and the
LA, but must have completed 40% of the  required reductions by 2004, 75% by 2009, and 100% by 2014 when the
final TMDL will be met. However, the TMDL is presently undergoing revision to incorporate findings from a new
model of Long Island Sound, and to reflect changes in dissolved O2 criteria in both states. The revised TMDL will
likely require more aggressive reductions of nitrogen to meet dissolved O2 criteria and may formalize targets for
upstream state contributions and atmospheric deposition.

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Appendix  H:
Nr  Saturation  and  Ecosystem  Function
   There are limits to how much plant growth can be
increased by N fertilization. At some point, when the
natural N deficiencies in an ecosystem are fully relieved,
plant growth becomes limited by availability of other
resources such as phosphorus, calcium, or water and the
vegetation can no longer respond to further additions of
Nr. In theory, when an ecosystem is fully Nr-saturated
and its soils, plants, and microbes cannot use or retain
any more, all new Nr deposits will be dispersed to
streams, groundwater, and the atmosphere. Nr saturation
has a number of damaging consequences for the health
and functioning of ecosystems. These impacts first
became apparent in Europe almost three decades ago
when scientists observed significant increases in nitrate
concentrations in some lakes and streams and also
extensive yellowing and loss of needles in spruce and
other conifer forests subjected to heavy Nr deposition. In
soils, most notably forest soils because of their natural
low pH, as NH4+ builds up it is converted to nitrate by
bacterial action, a process that releases hydrogen ions
and contributes to soil acidification. The buildup of NO3~
enhances emissions of nitrous oxides from the soil and
also encourages leaching of highly water-soluble NO3~
into streams or groundwater. As negatively charged NO3~
seeps away, positively charged alkaline minerals such as
calcium, magnesium, and potassium are carried along.
Thus, soil fertility is decreased by greatly accelerating
the loss of calcium and other nutrients that are vital
for plant growth. As calcium is depleted and the soil
acidified, aluminum ions are mobilized, eventually
reaching toxic concentrations that can damage tree roots
or kill fish if the aluminum washes into streams (Vitousek
etal.,  1997a,b).
   Forests, grasslands, and wetlands vary substantially
in their capacity to retain added nitrogen. Interacting
factors that are known to affect this capacity include
soil texture, degree of chemical weathering of soil,
fire history, rate at which plant material accumulates,
and past human land use. However, we still lack a
fundamental understanding of how and why N-retention
processes vary among ecosystems, much less how they
have changed and will change with time and climate
change (Clark and Tilman, 2008).
   An overarching impact of excess Nr on unmanaged
terrestrial ecosystems is biodiversity loss. In North
America, dramatic reductions in biodiversity have been
created by fertilization of grasslands in Minnesota
and California. In England, N fertilizers applied to
experimental grasslands have led to similarly increased
dominance by a few N-responsive grasses and loss
of many other plant species. In formerly species-rich
heathlands across Western Europe, Nr deposition has
been blamed for great losses of biodiversity in recent
decades, with shallow soils containing few alkaline
minerals to buffer acidification (Vitousek et al., 1997a,b;
Bobbinketal., 2010).
   Losses of biodiversity driven by Nr deposition can in
turn affect other ecological processes. Experiments in
Minnesota grasslands showed that in ecosystems made
species-poor by fertilization, plant productivity was much
less stable in the face of a major drought. Even in non-
drought years, the normal vagaries of climate produced
much more year-to-year variation in the productivity of
species-poor grassland plots than in more diverse plots
(Vitousek etal., 1997a,b).

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