ELECTROCHEMICAL SENSORS FOR
   ENVIRONMENTAL MONITORING:
A REVIEW OF RECENT TECHNOLOGY
                       by
                   JOSEPH WANG
             Department of Chemistry and Biochemistry
                 New Mexico State University
                Las Cruces, New Mexico 88003
                    Solicitation No.
                     LV-94-012
                    Project Officer
                     Kim Rogers
                   EMSL-U.S. EPA
                    P.O. Box 93478
                 Las Vegas, NV 89139-3478
              National Exposure Research Laboratory
               Office of Research and Development
              U.S. Environmental Protection Agency
               Research Triangle Park, NC 27711

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                                               Notice

    The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD),
funded and managed in the compilation of this research review.  It has been subjected to the Agency's peer review
and has been approved as an EPA publication. The U.S. Government has the right to retain a non-exclusive,
royalty-free license in and to any copyright covering this article.

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                                               Abstract

    Electrochemical sensors are expected to play an increasing role in environmental monitoring.  Significant
technological advances during the 1980's and early 19 90's are certain to facilitate the environmental applications
of electrochemical devices.  This report surveys important advances in electrochemical sensor technology,
including amperometric or potentiometric biosensors,  chemically modified electrodes, stripping-based metal
sensors, and other tools for on-site field testing. Such devices should allow one to move the measurement of
numerous inorganic and organic pollutants from the central laboratory to the field, and to perform them rapidly,
inexpensively, and reliably. Representative environmental applications and future prospects are discussed.
                                                    in

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                                           Contents

Notice	  ii
Abstract	iii
Introduction	  1
Principles	  2
Electrochemical Biosensors 	  3
Chemically Modified Electrodes for Environmental Monitoring  	  6
Stripping-based Metal Sensors	  8
Ion and Gas Selective Electrodes  	  11
Conclusions	  12
References	  13
                                            Tables

Table 1.  Typical Environmental Applications of Stripping Analysis	  9
Table 2.  Examples of Electrochemical Sensors and Biosensors for Environmental Analysis	  12
                                           Figures

1. Electrochemical biosensors: biorecognition and signal transduction	  3
2. Enzyme (tyrosinase) electrode for monitoring phenolic compounds  	  4
3. Amperometric immunosensor based electroactive-(A) and enzyme (B) tagged antigen 	  5
4. Electrocatalysis at modified electrodes; electron transfer mediated reaction between
   the target analyte and surface-bound catalyst	  7
5. Steps in anodic (A) and adsorptive (B) stripping voltammetry of trace metals, based
   on electrolytic and adsorptive accumulation, respectively, of target metal analytes	  10
                                               IV

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                                             Introduction
    Electroanalytical   chemistry   can  play  a  very
important role in the protection of our environment. In
particular, electrochemical sensors and detectors are very
attractive for on-site monitoring of priority pollutants, as
well as for addressing other environmental needs.  Such
devices satisfy many of the requirements for on-site
environmental analysis.  They are inherently sensitive
and selective  towards electroactive species, fast and
accurate,  compact, portable and  inexpensive.   Such
capabilities have already made a significant impact on
decentralized clinical analysis. Yet, despite their great
potential   for  environmental   monitoring,   broad
applications of electrochemical  sensors for pollution
control are still in their infancy.

    Several electrochemical devices, such  as pH- or
oxygen electrodes, have been used routinely for years in
environmental  analysis.     Recent  advances  in
electrochemical sensor technology will certainly expand
the scope of these devices towards a  wide range of
organic and inorganic contaminants and will facilitate
their role in field analysis.  These advances include the
introduction of modified- or ultramicroelectrodes, the
design  of highly  selective chemical or  biological
recognition layers, of molecular devices or sensor arrays,
and  developments  in the  areas of microfabrication,
computerized instrumentation and flow detectors.

    The EPA's Office of Research and Development is
currently pursuing the development of environmental
monitoring  technologies  which  can expedite  the
characterization of hazardous waste sites in the U.S.
Relevant to  this objective, is the review and evaluation
of currently reported field analytical technologies. The
objective of this report is to describe the principles, major
requirements,   prospects,   limitations,   and  recent
applications of electrochemical sensors for monitoring
ground  or surface waters.   It is not a comprehensive
review of these topics, but rather focuses on the most
important advances and recently reported devices which
hold great promise for on-site water analysis.

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                                              Principles
    The  purpose of a chemical  sensor  is to provide
real-time  reliable  information  about  the  chemical
composition of its surrounding environment.  Ideally,
such a device is capable of responding continuously and
reversibly and does not perturb the sample. Such devices
consist  of a  transduction  element covered with  a
biological or chemical recognition layer.  In the case of
electrochemical  sensors,  the analytical information is
obtained from the electrical  signal that results from the
interaction of the target analy te and the recognition layer.
Different electrochemical devices can be used for the task
of environmental monitoring (depending on the nature of
the analyte, the character of the sample matrix, and
sensitivity or selectivity requirements).  Most of these
devices fall into two major categories (in  accordance to
the nature of the electrical signal):  amperometric and
potentiometric.

    Amperometric sensors are based on the detection of
electroactive  species  involved  in  the  chemical or
biological recognition process.  The signal transduction
process is accomplished by controlling the potential of
the working electrode at a  fixed value  (relative to a
reference electrode) and monitoring the current as a
function  of time.  The applied potential  serves  as the
driving force for the electron transfer reaction  of the
electroactive species.  The resulting current is a direct
measure of the rate of the electron transfer reaction.  It is
thus reflecting the rate of the recognition event, and is
proportional to the concentration of the target analyte.

    In potentiometric sensors, the analytical information
is obtained by converting the recognition process into a
potential signal, which is proportional (in a logarithmic
fashion)  to  the  concentration  (activity)  of  species
generated or consumed in the recognition event. Such
devices rely on the use of ion selective electrodes for
obtaining the  potential  signal.    A permselective
ion-conductive  membrane  (placed at the tip of the
electrode) is designed to yield a potential signal that is
primarily due to  the  target ion.   Such response  is
measured under conditions of essentially zero current.
Potentiometric  sensors are very  attractive  for  field
operations because of their high selectivity, simplicity
and low cost.  They are, however, less sensitive and often
slower than their amperometric counterparts. In the past,
potentiometric devices have been more widely used, but
the increasing  amount of research on amperometric
probes should gradually shift this balance.  Detailed
theoretical   discussion   on   amperometric   and
potentiometric  measurements are available  in many
textbooks and reference works.1"5

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                                  Electrochemical Biosensors
    The remarkable specificity of biological recognition
processes has led to the development of highly selective
biosensing devices. Electrochemical biosensors hold a
leading position among the bioprobes currently available
and hold great promise for the task of environmental
monitoring.  Such devices consist of two components:  a
biological entity that recognizes the target analyte and
the electrode transducer that translates the biorecognition
event into a useful electrical signal. A general schematic
diagram for the operation of electrochemical biosensors
is  shown in Figure 1.  A great variety of schemes for
implementing the electrochemical biosensing approach,
based on different combinations of biocomponents and
electrode transducers have been suggested. These rely on
the immobilization of enzymes, antibodies, receptors or
whole  cells  onto amperometric   or potentiometric
electrodes. Fundamental aspects of these devices have
been reviewed in the literature.6"8

    Enzyme electrodes have the longest tradition in the
field of biosensors. Such devices are usually prepared by
attaching an enzyme layer to the electrode surface, which
monitors changes occurring as a result of the biocatalytic
reaction amperometrically or potentiometrically.  Am-
perometric enzyme electrodes rely on the biocatalytic
generation or consumption  of electroactive species.  A
large number of hydrogen-peroxide generating oxidases
and  NAD+-dependent  dehydrogenases  have  been
particularly useful  for the measurement of a wide range
of substrates  The liberated peroxide or NADH species
can be readily detected at relatively modest potentials
(0.5-0.8V vs. Ag/AgCl), depending upon the working
electrode  material.    Lowering  of  these  detection
potentials is desired for minimizing interferences from
coexisting electroactive species. Potentiometric enzyme
electrodes rely on the use of ion-  or gas-selective
electrode transducers, and thus allow the determination
of substrates whose biocatalytic reaction results in local
pH changes or the formation or consumption of ions or
gas (e.g. NH4+ or CO2). The resulting potential signal
thus  depends  on  the  logarithm  of  the  substrate
concentration. Proper functioning of enzyme electrodes
is greatly dependent on the immobilization procedure.

    The design of enzyme electrodes is  such that the
current or potential measured is proportional to the rate
limiting step in the  overall reaction.  For reactions
limited by the Michaelis-Menten kinetics, a leveling off
of calibration curves  is expected  at high substrate
concentrations. Mass-transport limiting membranes can
be used to greatly extend the linear range.  This will also
lead to a slower response. The  signal may be  dependent
also upon the pH of the water sample or its heavy-metal
content that affect the  enzymatic activity.   Attention
should be given also to  the long-term  stability of these
devices,  due to the limited  thermostability  of  the
biocatalytic layer. Improved immobilization and use of
thermophilic or 'synthetic' enzymes should be useful for
                     TARGET
                     INALYTE
          AMPEROMETRIC
          POTENTIOMETRIC SIGNAL
          CONDUCTOMETRIC
                                  ELECTROCHEMICAL SENSOR
Figure 1. Electrochemical biosensors: biorecognition and signal transduction.

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extending the lifetime of enzyme electrodes (particularly
in connection with field applications). Mass producible,
disposable enzyme electrodes can be readily fabricated
(as  common for clinical serf-testing of blood glucose),
and used as  'one-shot' throwaway devices.

    Several enzyme electrodes have  already  proven
useful for the task of environmental monitoring.  For
example,  several groups reported on highly sensitive
amperometric biosensors  for  phenolic  compounds.9"15
Such devices rely on the immobilization of tyrosinase
(polyphenol  oxidase)  onto   carbon-   or  platinum
transducers, and the low potential detection of the
liberated quinone product (Figure 2). Assays of industrial
wastes or natural water  have been documented,12"14
including  possible  remote  phenol   sensing13  and
single-use on-site sensing.14'15  Similarly, low potential
biosensing of organic peroxides or hydrogen peroxides
can  be   accomplished   at   peroxidase-modified
electrodes.16'17   "Class-selective"  enzyme  electrodes,
based on tyrosinase or peroxidase, can be used for semi-
quantitative field screening. They can also be used as
detectors for liquid chromatography, hence providing
quantitation of  the  individual substrates.18   The or-
ganic-phase activity of these enzymes should be useful
not only for chromatographic separations, but also in
connection  with rapid solvent extraction procedures.
Other enzymes, such as sulfite oxidase, nitrate reductase,
nitrilase,  alcohol dehydrogenase,  or formaldehyde
dehydrogenase have been employed for electrochemical
biosensing of environmentally-relevant species such as
sulfite,19   nitrate,20   organonitriles,21   alcohols,22  or
formaldehyde,23 respectively. Most of the above devices
offer low (micromolar) detection limit, good precision
(RSD = 1-3%) and fast (30-60 sec.) response.

    In addition to substrate monitoring, it is possible to
employ enzyme electrodes for measuring various toxins
(via the perturbation/modulation of the enzyme activity).
For  example,  the  inhibition  of enzymes,  such as
cholinesterase, tyrosinase, orperoxidase, has led to useful
biosensors  for  organophosphates  and  carbamates
pesticides,24 cyanide,25 or toxic metals.26'27 The resulting
(inhibition) plots thus  reflect the enzyme  inhibition
kinetics.   Such enzyme inhibition devices may thus be
useful  as early warning poison detectors.   Improved
specificity may be achieved by designing multi-enzyme
arrays that offer a "fingerprint" pattern of the individual
inhibitors. Analogous detection of benzene or herbicide
contaminations  and  of anionic  surfactants  can be
accomplished by   immobilizing  whole  cells  onto
electrodes and  monitoring  the  modulation  in the
microbial  activity.28'29'30    Another  environmentally
important microbial sensor offers rapid estimate of BOD
(biochemical oxygen demand), hence replacing the long
(5 day) conventional BOD test.31 The use of whole cells
(instead  of isolated enzymes) can increase the sensor
stability and allows regeneration of the bioactivity (via
immersion  in a nutrient media).  Other whole cell
electrodes, relying on plant tissues (such as mushroom or
horseradish) have been used for detecting phenolic and
peroxide substrates  (of their tyrosinase and peroxidase
enzymes).  While offering  prolonged lifetimes, such
tissue electrodes may suffer from side reactions due to the
coexistence of several enzymes.
                                     QUINONE
 Figure 2. Enzyme (tyrosinase) electrode for monitoring phenolic compounds.

                                                     4

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    Affinity  electrochemical  biosensors,  employing
natural binding molecules  as the recognition element
should also play a growing role in future environmental
monitoring.  In this  case  the  recognition process is
governed primarily by the shape and size of the receptor
pocket and the analyte of interest. Particularly promising
are electrochemical immunosensors due to the inherent
specificity of antibody-antigen reactions.32  Disposable
immunoprobes based on mediated electrochemistry have
been developed.33  In addition to immunosensors, the
environmental arena may benefit from the production of
electrochemical immunoassay test kits.   Such assays
commonly rely on labelling the antigen with an elec-
troactive tag (Figure 3 A), or with an enzyme that acts on
a substrate and liberates an electroactive product (Figure
3B). A wide variety of enzymes are suitable (peroxidase,
alkaline phosphatase, etc.), and there is also a wide
choice of substrates for these enzymes.  New test kits,
developed for the clinical market, may be readily adapted
for environmental monitoring. Other pro raising concepts
are   based   on   specific   binding   between
membrane-embedded receptors and target analytes34 or
the hybridization of electroactive markers by  surface-
bound DNA.35     Amperometric  or potentiometric
transducers are useful to follow these binding events.
Genetic  engineering technology  is  currently  being
explored for designing binding molecules  for  target
analytes.
                           W.E.
                                    Electroactive Labeled Antigens
                          W.E..
                         B
                                                                             Aa
                                      Enzyme Labeled Antigens
Figure 3. Amperometric immunosensor based electroactive-(A) and enzyme (B) tagged antigen.

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           Chemically Modified Electrodes for Environmental Monitoring
    Chemical layers can also be used for imparting a
high degree of selectivity to electrochemical transducers.
While conventional  amperometric  electrodes  serve
mainly for carrying  the  electrical current,  powerful
sensing  devices  can  be  designed  by  a  deliberate
modification  of  their   surfaces.     Basically,  the
modification of an electrode involves immobilization (on
its surface) of reagents that change the electrochemical
characteristics of the bare surface. Inclusion of reagents
within the electrode matrix (e.g. carbon paste) is another
attractive approach for modifying electrodes.   Such
manipulation  of the  mole-cular composition  of the
electrode thus allows one to tailor the response to meet
specific sensing needs. The new "mercury-free" surfaces
address also growing concerns  associated with field
applications of the classical mercury  drop electrode.
Theoretical details on modified electrodes can be found
in several reviews.36"38

    While sensors based on modified electrodes are still
in the early stages  of their lifetime, such preparation of
structured interfaces holds great promise for the task of
environmental monitoring. There are different directions
by which the resulting modified  electrodes can benefit
environmental  analysis,  including  acceleration  of
electron-transfer reactions, preferential accumulation or
permselective transport.

    Electrocatalysis   involves  an  electron  transfer
mediation between the target analyte and the surface by
an immobilized catalyst (Figure 4). Such catalytic action
results in faster electrode reactions at lower operating
potentials.  Various catalytic surfaces have thus been
successfully employed for facilitating the detection of
environmentally-relevant analytes (with otherwise slow
electron-transfer kinetics).  These  include the electro-
catalytic determination of hydrazines39 ornitrosamines40
at electrodes coated with mixed-valent ruthenium films,
monitoring of aliphatic aldehydes at palladium-modified
carbon paste,41 sensing of nitrite  at  a glassy  carbon
electrode coated with an osmium-based redox polymer,42
of nitrate at a copper modified  screen printed  carbon
electrode,43  monitoring of organic peroxides  at  co-
balt-phthalocyanine containing carbon pastes,44 and of
hydrogen   peroxide  at   a  copper  heptacyano-
nitrosylferrate-coated electrode.45

    Preconcentrating modified electrodes can also be
useful for environmental  sensing.   In  this case  an
immobilized reagent (e.g. ligand, ion-exchanger) offers
preferential uptake of target analytes. This approach
enjoys high sensitivity because it is a preconcentration
procedure. A second major advantage lies in the added
dimension of selectivity,  which is provided by the
chemical  requirement   of   the   modifier-analyte
interactions. Such improvements have been documented
for the measurement of nickel, mercury, or aluminum
ions at dimethylglyoxine,46 crown-ether,47 or alizarin48
containing carbon pastes,  respectively, monitoring of
nitrite, chromium, or uranyl  ions  at  ion-exchanger
modified   electrodes,49"51   and  of  copper   at   an
algaemodified electrode.52  Covalent reactions  can be
used for  analogous collection/determination of organic
analytes,  e.g.  monitoring  of  aromatic  aldehydes at
amine-containing  carbon   pastes.53     Routine
environmental applications of these  preconcentrating
electrodes would require attention to competition for the
surface site and the regeneration of an  'analyte-free'
surface.

    Another promising avenue is to cover the sensing
surface   with   an   appropriate permselective   film.
Discriminative  coatings  based on  different transport
mechanisms (based on analyte size, charge, or polarity)
can thus be used for addressing the limited selectivity of
controlled-potential probes in complex environmental
matrices.   The  size-exclusion sieving  properties of
various polymer-coated electrodes offer highly selective
detection of small  hydrogen  peroxide  or hydrazine
molecules.54'55  In addition, surface passivation (due to
adsorption of macromolecules present in natural waters)
can be prevented via the protective action of these films.

    More powerful sensing devices may result from the
coupling   of   several   functions   (permselectivity,
preconcentration or catalysis) onto the same surface.
Additional advantages can be  achieved  by designing
arrays of independent modified electrodes, each coated

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with a different  modifier and hence tuned toward a
particular  group  of analytes.   The resulting  array
response offers a  unique fingerprint  pattern of the
individual analytes, as well as multicomponent analysis
(in connection  with  statistical,  pattern-recognition
procedures).  Use of different permselective coatings or
catalytic   surfaces  thus  hold  great  promise  for
multiparameter pollution monitoring.  The development
of electrochemical sensor arrays has  been reviewed
               recently.56  Related to this are new molecular devices
               based on the coverage of interdigitated microarrays with
               conducting polymers.57'58  Eventually we expect to see
               molecular devices in which the individual components
               are  formed by discrete  molecules.   Modification of
               miniaturized  screen-printed sensor strips can also be
               accomplished via the inclusion of the desired reagent
               (e.g. ligand, catalyst) in the ink used for the micro-
               fabrication process.
                       Aox
                       Ared
Mred
Mox
Figure 4. Electrocatalysis at modified electrodes; electron transfer mediated reaction between the target analyte and
         surface-bound catalyst.

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                                Stripping-based Metal Sensors
    The  most  sensitive electroanalytical  technique,
stripping analysis, is highly suitable for the task of field
monitoring of toxic metals. The remarkable sensitivity
of stripping analysis is attributed to its preconcentration
step, in which trace metals are accumulated onto the
working electrode.  This step is followed by the stripping
(measurement) step, in which the metals are "stripped"
away from the electrode during an appropriate potential
scan. About 30 metals can thus be determined by using
electrolytic   (reductive)   deposition   or  adsorptive
accumulation of a  suitable  complex onto  the electrode
surface (Figure 5).  Stripping electrodes thus represent a
unique type of chemical sensors, where the recognition
(accumulation) and transduction (stripping) processes are
temporally resolved.  Short accumulation times (of 3-5
min) are thus sufficient for convenient quantitation down
to the  sub-ppb level,  with shorter periods (1-2 min)
allowing   measurements  of  ppb   and  sub-ppb
concentrations.  The timeconsuming deaeration step has
been eliminated by using modern stripping modes (e.g.
potentiometric or square-wave stripping), that  are not
prone to  oxygen interferences.  Stripping analysis can
provide useful information on the total metal content, as
well as  characterization of its  chemical form (e.g.
oxidation  state,   labile   fraction,  etc.).59    Field
measurements of  chromium(VI)  represent one such
example.60'61   Overlapping  peaks,   formation  of
intermetallic  compounds   and surfactant  adsorption
represent  the  most common  problems  in stripping
analysis.

    Various  advances in  stripping  analysis  should
accelerate the realization of on-site environmental testing
of toxic metals. New sensor technology has thus replaced
the traditional laboratory-based mercury electrodes and
associated  cumbersome  operation  (oxygen removal,
solution  stirring,  cell cleaning,  etc.).   Of particular
significance are new stripping-based  tools,  such as
automated   flow   systems  for   continuous   on-line
monitoring,62"64   disposable  screen-printed stripping
electrodes  for  single-use  field   applications,65  or
remote/submersible  devices  for  down-hole  well
monitoring or unattended operation.66'67 Portable and
compact  (hand-held),   battery-operated   stripping
analyzers are  currently  being  commercialized for
controlling these field-deployable devices. In addition to
providing on-site  realtime information,  such in-situ
devices should minimize errors (due to contamination or
loss)  inherent to  trace  metal  measurement  in  a
centralized laboratory.   Stripping analysis has  been
extensively used by marine chemists on board ships for
numerous oceanographic surveys.62 Relevant examples
of environmental applications of stripping analysis are
given in Table 1.

    In addition to trace metal pollutants, it is possible to
employ  new  adsorptive  stripping  procedures for
measuring  low levels of organic  contaminants that
display surface-active properties  (e.g.  detergents, oil
components).  However, due to competitive adsorption
such schemes  usually require a prior separation step.
Another version of stripping analysis, cathodic stripping
voltammetry,  can  be   used   for  measuring
environmentally-relevant anions (e.g.  S"2,  I",  Br") or
sulfur- or chlorine-containing pollutants (e.g. pesticides)
following their oxidative deposition onto the working
electrode.   Additional  background  information  on
stripping analysis and its environmental opportunities
can be found in various books or reviews.77"80

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              e
The Accumulation Step
                    M+n + ne" -»M
                                   Time
               e
          o
          CL
             ~acc
                      Accumulation
                                  ML
      MLn -» MLn,ads
                                              The Stripping Step

                                                   M -^ fvfn + ne"
                                Stripping
                                                                          B
                                                              Time
Figure 5. Steps in anodic (A) and adsorptive (B) stripping voltammetry of trace metals, based on electrolytic and adsorptive
        accumulation, respectively, of target metal analytes.

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Table 1. Typical Environmental Applications of Stripping Analysis
Trace Metal
As
Cd
Cr
Cu
Hg
Mn
Ni
Pb
Se
Tl
U
Matrix
Natural waters
Lakes and Oceans
Seawater
Sediments
Tap water
Seawater
Natural waters
Seawater
Lakes and Oceans,
Sediments
River water
Natural waters
Groundwater Sediments
Electrode
Gold
Mercury film
Mercury drop
Mercury film
Gold
Mercury drop
Mercury drop
Mercury film
Mercury film
Gold
Mercury film
Mercury drop
Stripping Mode
Differential pulse
Differential pulse
Adsorptive
Potentiometric
Differential pulse
Potentiometric
Adsorptive
Differential pulse
Potentiometric
Potentiometric
Differential pulse
Adsorptive
Ref.
68
65,69
61
60
70
71
72
73
65,69
60
74
75
76
                                                             10

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                               Ion and Gas Selective Electrodes
  Ion selective electrodes  offer direct and  selective
detection of ionic activities in water samples.   Such
potentiometric devices are simple, rapid, inexpensive and
compatible  with  on-line  analysis.    The  inherent
selectivity of these devices is attributed to highly selective
interactions between the membrane material and the
target ion.  Depending on the nature of the membrane
material  used to impart the  desired  selectivity, ion
selective  electrodes can be divided into three groups:
glass, solid, or liquid electrodes.   Many ion  selective
electrodes are commercially available and routinely used
in various fields.

  By far the most widely used ion selective electrode is
the pH electrode.  This glass-membrane sensor has been
used for  environmental pH measurements for several
decades.   Its  remarkable  success  is  attributed to its
outstanding analytical performance, and in particular to
its extremely high selectivity for hydrogen ions, broad
dynamic range, and fast and stable response.  Various
solid-state crystalline  membrane electrodes have been
shown useful for monitoring environmentally-important
ions, such as F, Br, OST, S"2 or Cu.+281  The calcium and
nitrate ion-exchanger sensors represent environmentally
useful liquid membrane electrodes. The synthetic design
of macrocyclic polyether ionophores has led  to liquid
membrane electrodes for heavy metals, such as lead or
zinc.82  Anion selective liquid membrane electrodes have
been developed in recent years for sensing of phosphate
or  thiocyanate.   New  technologies  of thin  film
(dry-reagent) slides or semiconductor chips will certainly
facilitate field  monitoring of ionic  analytes.83   The
principles and applications of ion selective electrodes
have been reviewed.84"86

  The rapid detection of ammonia or oxygen plays a vital
role in pollution control.  Gas sensing electrodes are
highly selective devices for monitoring these (and other)
gases.     Such  sensors  commonly  incorporate  a
conventional ion selective electrode,  surrounded by an
electrolyte solution and enclosed  by a gas permeable
membrane.   The  target gas  diffuses  through  the
membrane and reacts with the internal electrolyte, thus
forming or consuming a detectable ionic species.  The
ammonia  selective  probe uses an internal pH  glass
electrode in connection with an  ammonium chloride
electrolyte.  The glass electrode detects the decreased
activity  of protons.  While  most  gas sensors  rely on
potentiometric detection, the important oxygen probe is
based on covering an amperometric  platinum cathode
with a Teflon or silicon rubber membrane. Handheld
and remote oxygen probes are available commercially.87
Potentiometric sensors for other gases (SO2, NO2, HF,
etc.) have been designed by using  different membranes
and equilibrium processes.
                                                   11

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                                             Conclusions
  Electrochemical sensor technology is still limited in
scope,  and hence  cannot  solve all environmental
monitoring needs.  Yet, a vast array of electrochemical
sensors have been applied in recent years for monitoring
a wide range of inorganic and organic pollutants (Table
2).  We are continuously witnessing the introduction of
new electrochemical sensing devices, based on a wide
range of chemical or biological recognition materials. In
addition, mass production techniques (adapted from the
microelectronic  industry) enable  the fabrication  of
extremely small and reproducible, and yet inexpensive
(disposable), sensing devices.  Such devices are being
coupled   with   light   and   user-friendly
microprocessor-based instrumentation.

  Fast-responding electrochemical sensors are also being
adapted  for  detection  in  on-line  monitoring   or
flow-injection  systems  (as  needed  for continuous
monitoring or field screening applications).   Other
advances of selective and stable recognition elements,
"smart"  sensors  and   molecular  devices,  remote
electrodes,  multiparameter   sensor   arrays  or
micromachining and nanotechnology, are certain to have
a major impact on pollution control.  Additional efforts
should  be  given  to   the   development   of   new
immobilization procedures (that increase the stability of
the biocomponent), to the design of new electrocatalysts
(that  facilitate  the  detection  of additional  priority
pollutants),  to the  replacement of classical mercury
electrodes with well-defined solid surfaces, to address the
fouling and  degradation of  electrochemical  sensors
during use, to the development of immunoassay-based
electrochemical  sensors and of remote electrodes for
unattended operations, and introduction of multi-sensor
systems for simultaneous monitoring of several priority
contaminants.   On-going commercialization efforts,
coupled with regulatory acceptance, should lead to the
translation of these and future research efforts into large
scale environmental applications.
Table 2.  Examples of Electrochemical Sensors and Biosensors for Environmental Analysis
Analyte
Benzene
Cyanide
Hydrazines
Lead
Mercury
Nickel
Nitrite
Nitrosamines
Peroxides
Pesticides
Phenol
Sulfite
Uranium
Recognition
Modulated microbial activity
Enzyme inhibition
Electrocata lysis
Ion recognition
Preconcentration
Preconcentration
Preconcentration
Electrocatalysis
Biocata lysis
Enzyme inhibition
Biocata lysis
Biocatalysis
Preconcentration
Recognition
Process
Whole cell
Tyrosinase
Ruthenium catalyst
Macrocyclic ionophore
Crown ether
Dimethylglyoxine
Aliquat 336 ion exchanger
Ruthenium catalyst
Peroxidase
Acetylcholinesterase
choline oxidase
Tyrosinase
Sulfite oxidase
Nafion
Transduction
Element
Amperometry
Amperometry
Amperometry
Potentiometry
Voltammetry
Voltammetry
Voltammetry
Amperometry
Amperometry
Amperometry
Amperometry
Amperometry
Voltammetry
Ref.
mode
28
25
39
82
47
46
49
40
16,17
16,17
9-15
19
51
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