&EPA
     United States
     Environmental Protection
     Agency
    Technical Fact  Sheet-
                          1,4-Dioxane
                                  January 2013
                                                    TECHNICAL FACT SHEET - 1,4-DIOXANE
                                      Introduction
 At  a  Glance

 *  Flammable liquid and a fire hazard.
     Potentially explosive if exposed to
     light or air.
 *  Found at many federal facilities
     because of its widespread use as a
     stabilizer in chlorinated solvents,
     paint strippers, greases, and
     waxes.
 *  Short-lived in the atmosphere, may
     leach readily from soil to ground
     water, migrates rapidly in ground
     water, and is relatively resistant to
     biodegradation in the subsurface.
 *  Classified as "likely to be
     carcinogenic to humans" by all
     routes of exposure.
 *  Contact may cause eye and skin
     irritation and burns, coughing, or
     shortness of breath.
 *  No federal drinking water standards
     have been established. Many
     states and EPA regions have set
     guidelines and action levels.
 *  Modifications to existing sample
     preparation procedures may be
     needed to achieve the increased
     sensitivity needed for dioxane
     detection. High-temperature
     sample preparation techniques
     improve the recovery of dioxane.
 *  Common treatment technologies
     include advanced oxidation
     processes and ex situ
     bioremediation.
This fact sheet, developed by the U.S. Environmental Protection Agency
(EPA) Federal Facilities Restoration and Reuse Office (FFRRO),
provides a brief summary of the contaminant 1,4-dioxane, including
physical and chemical properties; environmental and health impacts;
existing federal and state guidelines; detection and treatment methods;
and additional sources of information.

1,4-Dioxane is a probable carcinogen and has been found in
groundwater at sites throughout the United States. The physical and
chemical properties and behavior of 1,4-dioxane create challenges for its
characterization and treatment. It is highly mobile and has not been
shown to readily biodegrade in the environment. This fact sheet is
intended for use by site managers faced with addressing 1,4-dioxane at
cleanup sites or in drinking water supplies and for those in a position to
consider whether 1,4-dioxane should be added to the analytical suite for
site investigations.

What is  1,4-Dioxane?

»>   1,4-Dioxane is a synthetic industrial chemical that is completely
    miscible in water (EPA 2006).
»>   Synonyms include dioxane, dioxan, p-dioxane, diethylene dioxide,
    diethylene oxide, diethylene ether, and glycol ethylene ether
    (Mohr2001).
»>   1,4-Dioxane is unstable at elevated temperatures and pressures and
    is potentially explosive if exposed to light or air (Alexeeff 1998).
»>   It is used as a stabilizer for chlorinated solvents such as 1,1,1-
    trichloroethane (TCA); a solvent for impregnating cellulose acetate
    membrane filters; a wetting and dispersing agent in textile
    processes; and as a laboratory cryoscopic solvent for molecular
    mass determinations (ATSDR 2012; EPA 2006).
»>   It is used in many products, including paint strippers, dyes, greases,
    varnishes, and waxes. 1,4-Dioxane is also found as an impurity in
    antifreeze and aircraft deicing fluids and in some consumer products
    [deodorants, shampoos, and cosmetics] (ATSDR 2012; EPA 2006;
    Mohr2001).
»>   1,4-Dioxane is a likely contaminant at many federal facilities because
    of its widespread use.
»>   Residues may be present in manufactured food additives, 1,4-
    dioxane-containing food packaging materials,  or on food crops
    treated with pesticides that contain 1,4-dioxane [such as, vine-
    ripened tomatoes] (DHHS 2011).
»>   It is also a by-product in the manufacture of polyethylene
    terephthalate (PET) plastic and is used as a purifying agent in the
    manufacture of Pharmaceuticals (Mohr2001).
United States
Environmental Protection Agency
       Office of Solid Waste and
       Emergency Response (5106P)

             1
EPA 505-F-13-001
    January 2013

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  Technical Fact Sheet - 1,4-Dioxane
What is  1,4-Dioxane? (continued)
                      Exhibit 1: Physical and Chemical Properties of 1,4-Dioxane
                                    (ATSDR 2012; EPA IRIS 2010)

CAS Number
Physical Description (physical state at room temperature)
Molecular weight (g/mol)
Water solubility (mg/L at 25°C)
Boiling point (°C)
Vapor pressure at 25°C (mm Hg)
Specific gravity
Octanol-water partition coefficient (log Kow)
Organic carbon partition coefficient (log Koc)
Henry's law constant at 25 °C (atm cm3/mol)

000123-91-1
Flammable liquid with a faint, pleasant odor
88.11
Soluble in water
101. 1 "Cat 760 mm Hg
38.1
1.033
-0.27
1.23
4.80 X10'6
       Notes: g/mol - grams per mole; mg/L - milligrams per liter; °C - degrees Celsius; mm Hg - millimeters of mercury;
       atm m3/mol - atmosphere-cubic centimeters per mole.

What are the environmental  impacts  of 1,4-Dioxane?	
    1,4-Dioxane is typically found at solvent release
    sites and PET manufacturing facilities
    (Mohr2001).
    It is short-lived in the atmosphere, with a 6- to 10-
    hour half-life (Mohr2001). Breakdown products
    include aldehydes and ketones.
    It may migrate rapidly in groundwater, ahead of
    other contaminants, and does not volatilize rapidly
    from surface water bodies (EPA 2006).
It is weakly retarded by sorption to soil particles
and may move rapidly from soil to groundwater
(EPA 2006).
It is relatively resistant to biodegradation and does
not bioconcentrate in the food chain (ATSDR
2012; Mohr2001).
It has been detected in at least 31 of the 1,689
current or former sites on EPA's National Priorities
List (NPL); it may be present (but samples were
not analyzed for it) at many other sites (ATSDR
2012).
What are the health effects of 1,4-Dioxane?
    Potential exposure could occur during production
    and use of 1,4-dioxane as a stabilizer or solvent
    (DHHS2011).
    Exposure may occur through inhalation of vapors,
    ingestion of contaminated food and water, or
    dermal contact (DHHS 2011).
    Inhalation is the most common route of human
    exposure; it is also readily adsorbed through the
    lungs, skin, and gastrointestinal tract. Distribution
    is rapid and uniform in lung, liver, kidney, spleen,
    colon, and skeletal muscle tissue (ATSDR 2012).
    Workers at industrial sites are at greatest risk of
    repeated inhalation exposure (DHHS 2011).
    Short-term exposure may result in irritation of the
    eyes, and throat (ATSDR 2012).
    Chronic exposure may result in dermatitis,
    eczema, drying and cracking of skin, and liver and
    kidney damage (ATSDR 2012; EPA OSW1996).
1,4-Dioxane is weakly genotoxic and reproductive
effects are unknown; however, a developmental
study on rats indicated that the developing fetus
may be a target of toxicity (ATSDR 2012; EPA
IRIS 2010).
The oral slope factor for carcinogenic risk is
1 x 10~1 milligrams per kilogram per day (mg/kg-
day) and the drinking water unit risk is 2.9 x 10~6
per microgram per liter (ug/L). EPA has classified
1,4-dioxane as "likely to be carcinogenic to
humans" by all routes of exposure (EPA IRIS
2010).
The chronic oral reference dose (RfD) is
0.03 mg/kg-day (EPA IRIS 2010).
The EPA's drinking water equivalent level (DWEL)
is 1 milligrams per liter (mg/L) (EPA 2012a).

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  Technical Fact Sheet - 1,4-Dioxane
Are there any federal and state standards and guidelines for 1,4-Dioxane?
    Federal and State Standards and Guidelines:
    •  1,4-Dioxane may be regulated as hazardous
      waste when used as a solvent stabilizer (EPA
      OSW1996).
    •  No federal drinking water standards have been
      established (DHHS  2011; ATSDR 2012).
      However, a maximum contaminant level (MCL)
      is not necessary to determine a cleanup level.
    •  A minimal risk level  (MRL) of 2 parts per million
      (ppm) was derived for acute-duration inhalation
      exposure, 0.2 ppm for intermediate-duration
      inhalation exposure, and 0.03 ppm for chronic-
      duration inhalation exposure (ATSDR 2012).
    •  A MRL of 5 mg/kg/day was derived for acute-
      duration oral exposure, 0.5 mg/kg-day for
      intermediate-duration oral, and 0.1 mg/kg-day
      for chronic-duration  oral exposure (ATSDR
      2012).
    •  EPA established a 1-day health advisory of 4.0
      mg/L and a 10-day health advisory of 0.4  mg/L
      for 1,4-dioxane based on a 10-killiogram (kg)
      child (EPA 2012a).
    •  EPA has calculated a screening level of
      6.7 x 10~1 ug/L for 1,4-dioxane in tap water,
      based on a 1 in 10~6 lifetime excess cancer risk.
      This screening level is not enforceable but
      provides a useful gauge of relative toxicity (EPA
      2012c).
    •  EPA has calculated a residential soil screening
      level of 4.9 milligrams per kilogram (mg/kg) and
      an industrial soil screening level of 1.7 x 101
      mg/kg (EPA 2012c).
    •  EPA has also calculated residential air
      screening  level of 3.2 x 10~1  micrograms per
      cubic meter (ug/m3) and an  industrial air
      screening  level of 1.6 ug/m3 (EPA 2012c).
    •  The soil-to-groundwater risk-based soil
      screening  level (SSL) is 1.4 x10"4 mg/kg (EPA
      2012c).
    Workplace Exposure Limits:
    •  The Occupational Safety and Health
      Administration (OSHA) set an average 8-hour
      time-weighted average (TWA) airborne
   permissible exposure limit (PEL) of 360
   milligrams per cubic meter (mg/m3) or 100 mg/L
   (NIOSH 2007; OSHA 1998).
•  The American Conference of Governmental
   Industrial Hygienists (ACGIH) threshold limit
   value (TLV) for dermal exposure is 25 mg/L,
   and the recommended airborne exposure limit
   is 20 mg/L averaged over an 8-hour TWA
   (ACGIH 1998; EPA OSW 1996).
•  The National  Institute for Occupational Safety
   and Health (NIOSH) has set 500 mg/L as the
   concentration that is immediately dangerous to
   life or health (IDLH) and recommended 1  mg/L
   as the airborne exposure limit (NIOSH 2007).
Other State and  Federal Standards and
Guidelines:
•  State regulators often use drinking water action
   levels and health advisories to establish site
   cleanup goals. Cleanup levels vary by state,
   ranging from 3 to 50 ug/L in drinking water.
   Colorado has established an interim
   groundwater quality cleanup standard of 0.35
   ug/L (ATSDR 2012; CDPHE 2012).
•  The Food and Drug Administration set 10 ppm
   as the limit for 1-4-dioxane in glycerides and
   polyglycerides for use in  products such as
   dietary supplements.  FDA also surveys raw
   material and products contaminated with 1,4-
   dioxane (ATSDR 2012).
•  1,4-Dioxane is exempted from tolerances for
   pesticide chemicals in or on raw agricultural
   commodities, and it has been classified as a
   toxic inert ingredient of pesticide products
   (EPA 2012b).
•  1,4-Dioxane is listed as a hazardous air
   pollutant under the Clean Air Act (CAA) (EPA
   2012b).
•  A reportable quantity of 100 pounds has been
   established under CERCLA (EPA 2012b).

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 Technical Fact Sheet - 1,4-Dioxane
What detection and site characterization methods are available for
1,4-Dioxane?
   As a result of the limitations in the analytical
   methods to detect 1,4-dioxane, it has been difficult
   to identify its occurrence in the environment.
   Modifications to existing sample preparation
   procedures may be needed to achieve increased
   sensitivity for 1,4-dioxane detection (EPA 2006).
   Conventional analytical methods produced
   sensitivity levels that were about 100 times greater
   for 1,4-dioxane as compared with those for volatile
   organic compounds (VOCs) (Mohr2001).
High-temperature sample preparation techniques
improve the recovery of 1,4-dioxane. These
techniques include purging at elevated
temperature (SW-846 Method 5030C); equilibrium
headspace analysis  (SW-846 Method 5021);
vacuum distillation (SW-846 Method 8261A); and
azeotrophic distillation (SW-846 Method 5031)
(EPA 2000, 2006).
It is recommended that groundwater samples be
analyzed for 1,4-dioxane where TCA is a known
contaminant.
What technologies are being used to treat 1,4-Dioxane?
    Pump-and-treat (P&T) remediation is potentially
    applicable when the ex situ treatment is tailored for
    the unique properties of 1,4-dioxane
    (EPA 2006).
    Commercially available advanced oxidation
    processes (AOP)  using hydrogen peroxide with
    ultraviolet (UV) light or ozone is used to treat 1,4-
    dioxane (EPA 2006; EPA OSW 1996).
    Ex situ bioremediation using a fixed-film, moving-
    bed biological treatment system is also used to
    treat 1,4-dioxane  (EPA 2006).
    Phytoremediation is being explored as  a means to
    remove the compound from shallow  groundwater.
    Research on hybrid poplars has demonstrated
    their ability to take up and effectively degrade or
    deactivate 1,4-dioxane (EPA 2001, EPA 2012b).
Microbial degradation in engineered bioreactors
has been documented under enhanced conditions
or where selected strains of bacteria capable of
degrading 1,4-dioxane are cultured
(EPA 2006, 2010).
Photocatalysis has been shown to remove 1,4-
dioxane in aqueous solutions (Purifies ES Inc.
2006; Vescovietal. 2010).
Other in-well combined treatment technologies
being assessed include air sparging; soil vapor
extraction; and dynamic subsurface groundwater
circulation (Odah et al. 2005).
Where can I find  more information about 1,4-Dioxane?
   Agency for Toxic Substances and Disease
   Registry (ATSDR). 2012. "Toxicological Profile for
   1,4-Dioxane."
   www.atsdr.cdc.qov/toxprofiles/tp187.pdf
   Alexeeff, G. 1998. Office of Environmental Hazard
   Assessment. Memorandum: 1,4-Dioxane Action
   Level.
   http://oehha.ca.qov/water/pals/pdf/PAL14DIOXAN.
   Edf
   American Conference of Governmental Industrial
   Hygienists (ACGIH). 1998. Threshold Limit Values
   (TLVs) for Chemical Substances and Physical
   Agents Biological Exposure Indices for 1998.
   Cincinnati, Ohio.
Colorado Department of Public Health and the
Environment (CDPHE). 2012. Notice of Public
Rulemaking Hearing. Regulation No. 31 and No.
41.
http://www.sos.state.co.us/CCR/Upload/NoticeOfR
ulemakinq/ProposedRuleAttach2012-00387.PDF
International Agency for Research on Cancer
(IARC). 1999. "Re-Evaluation of Some Organic
Chemicals, Hydrazine and Hydrogen Peroxide."
Monographs on the Evaluation of Carcinogenic
Risk of Chemicals to Man. Volume 71. Pages 589
to 602.
Mohr, T.K.G. 2001. "Solvent Stabilizers White
Paper." Prepublication Copy. Santa Clara Valley
Water District of California. San Jose, California.

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 Technical Fact Sheet - 1,4-Dioxane
Where can I find  more information about 1,4-Dioxane? (continued)
    National Institute for Occupational Safety and
    Health (NIOSH). 2007. "Pocket Guide to Chemical
    Hazards." Cincinnati, Ohio. Page 120.
    www.cdc.gov/niosh/docs/2005-149/pdfs/2005-
    149.pdf
    Occupational Safety and Health Administration
    (OSHA). 1998. "Occupational Safety and Health
    Standards, Toxic and Hazardous Substances." 29
    Code of Federal Regulations 1910.1000.
    Odah, M.M., R. Powell, and D.J. Riddle. 2005.
    "ART in-well technology proves effective in
    treating 1,4-Dioxane contamination." Remediation
    Journal. Volume 15 (3), Pages 51 to 64.
    Purifies ES Inc. 2006. Case History: 1,4 Dioxane
    Remediation 1.
    U.S. Department of Health and Human Services
    (DHHS). 2011. 1,4-Dioxane, CAS No. 123-91-1,
    Report on Carcinogens, 12th Edition.
    U.S. Environmental Protection Agency (EPA)
    Office of Solid Waste (OSW). 1996. Solvents
    Study. EPA 530-R-96-017, 52 pages.
    EPA. 2000. "Method 8261. Volatile Organic
    Compounds by Vacuum Distillation in Combination
    with Gas Chromatography/Mass Spectroscopy
    (VD/GC/MS)." In: SW-846 Draft Update IVB.
EPA. 2001. "Brownfields Technology Primer:
Selecting and Using Phytoremediation for Site
Cleanup." EPA 542-R-01-006.
EPA. 2006. "Treatment Technologies for 1,4-
Dioxane: Fundamentals and Field Applications."
EPA 542-R-06-009.
www.epa.gov/tio/download/remed/542r06009.pdf
EPA. 2012a. 2012 Edition of Drinking Water
Standards and Health Advisories.
http://water.epa.gov/action/advisories/drinking/uplo
ad/dwstandards2012.pdf
EPA. 2012b. "1,4 Dioxane" www.clu-in.org/
contaminantfocus/default.focus/sec/1,4-
Dioxane/cat/Overview/
EPA. 2012c. Regional Screening Level (RSL)
Summary Table.
http://www.epa.gov/region9/superfund/prg/
EPA Integrated Risk Information System (IRIS).
2010. "1,4-Dioxane (CASRN 123-91-1)."
www.epa.gov/iris/subst/0326.htm
Vescovi T., H. Coleman, and R. Amal. 2010. "The
Effect of pH on UV-based advanced oxidation
technologies- 1,4-dioxane degradation." Journal of
Hazardous Materials. Volume 182. Pages 75 to
79.
Additional information on 1,4-dioxane can be found at www.cluin.org/dioxane.
Contact Information
If you have any questions or comments on this fact sheet, please contact: Mary Cooke, FFRRO, by phone at
(703) 603-8712 or by email at cooke.maryt@epa.gov.

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