Biological Study of the Fox and Wisconsin Rivera
                        by

                Douglas W. Grothe
              Charles S. Steiner Jr.
                 U.S.  EPA Region V
            Central  Regional Laboratory
                    October 1978

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                              INTRODUCTION

     At the request of the Water Division and in cooperation with the

Western District 'Office, the Central Regional Laboratory Biology Section

was asked to conduct a biological survey of the Fox and Wisconsin Rivers

at Portage, Wisconsin.  This was done in support of an Environmental Impact
                i
Statement (EIS) currently in preparation.

     This report includes biological findings during three different sampling

periods, namely June 12-16, July 10-13, and August 14-17, 1978.   In addition,

to general physical chemistry such as temperature, pH, dissolved oxygen and

specific conductance, the biological parameters collected include phytoplankton,

zooplankton, periphyton, macroinvertebrates, and chlorophyll.  During the

August sampling period, fish sampling was conducted on both rivers.

     It should be noted that on the last day of the June survey, the field

notebook describing the sampling locations and results of general chemical

analysis performed in the field was lost.  Biologists in the field reconstructed,

to the best of their knowledge, all information that was included in the

notebook.  Because most of the data had been collected within a  two day period,

the biologists are confident in their reconstruction descriptions of each

sampling location as well as the general chemical and physical data collected.

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                                 SUMMARY



     Biological conditions in the Wisconsin and Fox Rivers varied from



station to station and from one sampling period to another, resulting in



a shift from moderately enriched conditions (Mesotrophic)  to more seriously



enriched conditions (eutrophic).  The following is a brief classification



of each of the three stations studied on both rivers.



     1.  Station one on the Fox River  was considered to be moderately to



         heavily enriched, while the Wisconsin River at Station one was



         classified as being moderately enriched.



     2.  Both rivers at Station two appeared to be somewhat similar in terms



         of biological productivity during the June and July sampling period,
                I


         However,  in August, there seemed to be a  trend on the Fox River



         toward a  greater number of pollution tolerant forms,  while the



         Wisconsin River remained relatively constant.



     3.  The Wisconsin River at Station three was  more enriched as compared



         to the same station on the FOX River throughout most of. the study



         period.   During the latter part of the study both rivers showed a



         decline in water quality as  shown by the  disappearance of certain



         pollution intolerant forms.

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                          STATION DESCRIPTION




Fox River




     Station one was located approximately 3/4 of a mile downstream of




Swan Lake.  The River, at this point, was 20 meters wide with a depth of  one




meter.  Submerged aquatic vegetation covered the sampling area.  The bottom




substrate consisted of soft and "mucky" organic matter with numerous empty




snail shells.  The surrounding land was a marsh-type area with numerous




grasses, cattails, and shrubs lining the shoreline.




     Station two was located at Highway 33 East Bridge approximately one-




quarter of a mile downstream of the Portage Sewage Treatment Plant.  The




channel width was 15 meters, with a depth of one meter.  The bottom substrate




consisted of sand -    and "mucky" organic matter.  The shoreline was lined




with tall grasses and small shrubs.  Submerged aquatic vegetation covered




the sampling site.  At certain times of the year, duckweed lines the sides




of the channel.




     Station three was located downstream of the Portage Sewage Treatment




Plant at the Clark Street Bridge.  The channel width was 15 meters.  The




depth of the river was one meter with a bottom substrate consisting of  rock,




sand, "mucky" organic matter, and numerous empty snail shells.  'The shoreline




was lined v;ith grasses and cattails.  At times, duckweed lines both sides




of the channel and submerged aquatic vegetation covers the sampling site.









Wisconsin River




     Station one was located approximately one mile upstream of Highway 78




Bridge at the point where the river starts to bend on the left bank. An




Island was located one-half mile upstream of the station where the river




seperates into two channels.  Channel width was 250 meters.  The bottom




substrate consisted of fine to coarse sand.  Numerous trees and shrubs lined

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 the left bank of  the river, while a sandy shoreline with grasses lined

 the right bank.   Due to the width of the channel, three substations were
                  i
 located along a transect across the river.  Substations A and C were

 located 50 meters from the right and left bank respectively, while substation

 B was located in  the middle of the channel.

     Station two was located across from the radio station WPDR antenna
                 I
 on the right bank of the river.  The channel width was 200 meters.  The
                 i _,
 bottom substrate  consisted of fine to coarse sand.  Channel depth was between

 1.0-1.5 meters.  This section of the Wisconsin River consisted of numerous

 sandbars and islands interdispersed with very shallow areas.  The main flow

 of the river appeared to be along the left bank. The shoreline of both sides

was lined with numerous trees and shrubs.  Due to the width of the channel,

 three substations were taken along a transect across the channel.  Substations

 A and C were located forty meters from the right and left banks, respectively.

 Substation B was located in the middle of the channel.

     Station three was located adjacent to the public boat access landing

 at Dekorra Park.  The channel width was 200 meters with a bottom substrate

 consisting of fine to coarse sand.   Three substations were located along a

 transect across the river.   Substations A and C were located forty meters

from the right and left banks, respectively.   Substation B was located in

 the middle of the channel.   The depth at this station was 1.5-2.0 meters.

The shoreline along the left bank was lined with numerous trees, whereas the

right bank was lined with sandstone  cliffs.

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00
r-
                    ,₯PDR
                    flRadio tower
              DeKorra Park
              Boat Landing
                                 •t-n
                      and Wisconsin
                      saBPUng sites
                                           showing
                                                          l

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                                 METHODS
Macroinvertebrates
     Macroinvert^brates referred to in this report are aquatic organisms
that can be retained by a U.S. Standard No. 30 sieve (28 mesh per inch)  and
live at least part of their life cycle within or upon underwater substrates.
     Qualitative macroinvertebrate samples were obtained by washing organisms
from rocks, logs and other substrates at the sampling sites.  Organisms  were
washed into a Surber sampling net.  All organisms collected were placed  into
a quart glass jar and preserved with 5% formalin.
     Quantitative macroinvertebrate samples were obtained by using a Ponar
dredge.  The samples were washed in the field through a No.  30 mesh sieve.
All organisms collected were placed into a quart glass jar and preserved
with 5% formalin.  Two indepdendent samples were taken at each sampling  station.

Phytoplankton
     Phytoplankton refers to microscopic plants (algae) suspended in a
body of water that are incapable of sustained mobility in directions counter
to the water currents.
     Phytoplankton samples were obtained by taking a grab sample at the  waters
surface using a 500 ml plastic bottle.  Samples were preserved with lugols
solution.

Zpoplankton ,
     Zooplankton refers to the microscopic animals of the plankton community
which graze upon the phytoplankton as a source of food.
     Quantitative zooplankton samples were obtained by using an eight liter
Niskin bottle.   Four independent grab samples were obtained  approximately
one meter below the waters surface at each station.  The contents of each
                                     6

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Niskin bottle were then pooled.  The organisms in the pooled samples were




concentrated by passing the sample through a plankton funnel fitted with




a 53 v mesh net.  The sample was placed into a 500 ml plastic bottle,




appropriately labeled and preserved with 5% formalin.









Chlorophyll




     Chlorophyll refers to all plantlife containing a pigment known as




ehlorophylla_.  The measurement of this pigment can yield some insight  into




the relative amount of alga standing crop.  Chlorophyll samples  were prepared




In the field by filtering a known aliquot of water through a Gelman A/E




glass fiber filter.  A MgCO,, suspension was then filtered through to prevent




the sample from becoming too acidic.  The filter was immediately wrapped in




aluminum foil and placed inside a metal cannister which had been appropriately




labeled.  The sample was then placed on dry ice for transport back to  the




laboratory.









Periphyton




     Periphyton refers'to an assemblage of organisms that grow on underwater




subsrtates, and includes such organisms as algae, molds and protozoa.   All




of the organisms are not necessarily attached to the substrate but at  least




live in association with attached organisms.




     Periphyton were collected on l"x3" glass microscope slides  by means of




a periphytometer.  The length of exposure was four weeks, at which time the




slides were retrieved.  The slides collected were placed into a  100 ml




plastic bottle and preserved with lugols solution.

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Fish-Electrofishing




     A boat mounted pulsating direct current electrofishing unit was




utilized.  (Colfelt electronics model W-20).   The electric current was




directed into the water through a pair of steel cables which were suspended




from booms extending from the bow of the boat.  Each sampling area was




electrofished for a period of between 20-26 min.




     All stunned fish were dip-netted from the water and transferred  to  a




holding tank, where they recovered from the electroshocking.  All fish captured




were identified to species when possible, counted, measured, weighed,  and'




returned to the water.









Chemistry




     Dissolved oxygen and temperature were measured using a YSI model  54A




oxygen and temperature meter (Yellow Spring Instrument Co.).  The oxygen




meter was calibrated daily against the lodometric method for D.O. analysis




as outlined in Standard Methods for the Examination of Water and Wastewater,




14th edition 1975.   The pH was measured using  an Oion lonalyzer model  A07A




pH meter.  Specific conductance was measured using an Industrial Instruments




model RB3 Solu Bridge conductivity meter and probe.

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                            CUSTODY PROCEDURE

     Custody procedures were followed throughout the study.  All samples

collected at a particular station were immediately placed into ice chests.

While the stations were being sampled, ice chests were kept secured in a

government vehicle.  At the end of each day, all samples collected were

recorded onto custody sheets and signed.  Samples were returend to the ice
                j                                    /
chest and sealed with custody labels for transport back to the laboratory.

Back in the laboratory, custody procedures were followed as prescribed by the

Central Regional Laboratory Custody Manual.




                                RESULTS

Chemis try

     General chemistry data is presented in Table I.  As can be seen,

specific conductance concentrations were greater in the Fox River than the

Wisconsin River.  Between June and August, specific conductance concentrations

in the Fox River ranged between 360-560 pohms/cm compared to a range of

140-160 pohms/cm in the Wisconsin River.  The pH and temperature were similar

in the two rivers.  The two river systems did differ with regard to the amount

of dissolved oxygen present at the various stations.  In the Wisconsin River

dissolved oxygen concentrations remained between 7.1-8.8 mg/1.  However, in

the Fox River,  a significant oxygen sag was present at Stations two and three,

located downstream of the Portage Sewage Treatment Plant.

     In July and August, Station two had dissolved oxygen concentrations of

2.6 to 3.8 mg/1, while Station three had concentrations of 2.2-3,9 mg/1.

In June, Station two had a D.O, concentration of 7.0 mg/1.  This higher D.O.

concentration compared to the values obtained in July and August could be

due to the higher D.O. water from Station one (12.2 to 14.4) flowing into

Station Iv/o,,

                                    9

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Fish




     Results of the fish shocking are presented in Tables 2, 3, and 4.




Five species of fish were captured from station one on the Fox River.  The




most abundant species was Lepomis microlophus  (Redear sunfish).  A total




of four fish were caught.  The other species present were bowfin (Amia calva),




green sunfish (Lepomis cyanellus), brown bullhead (Ictalurus nebulosus), and




yellow perch (Perca flaves cens).  Fifteen young of the year sunfish were




also retrieved but these were not identified to species.




     In the Wisconsin River, only two species of fish were present at




Station one.  The most abundant fish present were minnows, which were not




identified to species.  One large (64 oz.) quilback carpsucker (Carpoides




cyprinus) was caught.  At Station two, four species of fish were captured.




Only one of each of these species was retained.  The species represented were




quilback carpsucker (Carpoides cyprinus), smallmouth bass (Ilicropterus




dolomieui),  yellow perch (Perca flavescens), and minnows.  Station three




was represented by the following fish species; quilback carpsucker (Carpoides




cyprinus), largemouth bass (Micropterus salmoides), silver redhorse (Moxustoma




an isurum) ,  and minnows.  Minnows were the most abundant group represented.









_Zoop_lank_toji




Fox River




     Except for June, Station one supported a considerably larger zooplankton




population than Station three (Table 5,6).  This was most evident in July




and August when Station one supported 161 and 188 org/1 respectively, compared




with Station three, which had only 34 org/1 in July and 17 org/1 in August.




Members of the Rotifera dominated the zooplankton present during the study.




The rotifera was most represented by Kgrate!la cochlearis, Polyarthra vulgaris,
                                     10

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Kera te 1 la earllnae and Trichocerca sp.  Members belonging to the order


copepoda were present in relatively low numbers.  The copepoda, during all


three months, were represented by species in the early form of development


(Cyclops juveniles and Nauplii).





Wi s cons in Ri ye r


     Throughout the study period, Station one supported a larger zooplankton


population than Station three.  This difference was most noticeable in


June and July.  In June, Station one had a total of 139 org/1 compared to


98 org/1 at Station three, while in July, Station one contianed 65 org/1


compared to 34 org/1 at Station three.  In August, there was only a slight


difference between Station one (28 org/1) and Station three (24 org/1) .


Members of the Rotifera dominated the zooplankton present during the study.


The Rotifera were represented by Keratella cochlearis, Syncharta sp.,
                                                    t

Trichocerca similis, and Polyarthra vulgaris.   The Copepoda were represented


by early life stages of Epecies (Cyclops juveniles and Nauplii).  These


species, however, never became numerically important.




Phy toplank ton


Fox River

       r
     Throughout the entire study period, Station one supported a considerably


larger phytoplankton population than Stations  two and three  (Tables  7, 8 and 9),


In June,  Sta,tion one was dominated by the blue-green algae, namely Anabaena sp. ,


and Aphanizomenon flos aquae.  However, later in the summer, a noticeable


change in the algal population occurred.  By July, the flagellated algae


consisting of Cryptomonas jsp_. , and other miscellaneous flagellate species


increased substantially from June (.612 cells/ml to 5860 cells/ml), thus


accounting for the dominance by both the blue-green algae and flagellates.


                                    11

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By August,  the  flagellate population  continued  to Increase reaching a

                                                                              •

concentration of  12,970 cells/ml.  The blue-green population had decreased



from  a  concentration of 7250 cells/ml in July to 1130 cells/ml in August.



Thus, in August the flagellate population dominated comprising 87.0 per



cent  of the population.



      Station two  showed shifts in population numbers and composition between



June  and August.  In terms of cell concentration in June, Station two supported



a population of 5253 cells/ml.  However, by July, the numbers of phytoplankton



encountered decrease to 2940 cells/ml and by August to 2720 cells/ml.  During



this  same time period, changes in the dominant' algal forms also changed.



In June, the centric diatoms consisting of Cyclotella spp. , comprised 68%



of the  species, however, by July the  flagellated algae "became dominant (62%)



and this trend continued through the  August study.



      Station three, unlike Station two, showed an increase in the phytoplankton



population from June until August.  In June, the algal cell concentration



was 2907 cells/ml.  By July, the algae had increased to 6230 cells/ml, and



in August, to 10,940 cells/ml.  With  regard to species composition, the June



study showed a diverse population of  all major groups of algae with no one



form  showing complete dominance.  However, by July, the flagellated algae



(consisting of Cryptomonas jsjuand misc. flagellates) began to increase in



numbers and became the dominant algae.  This group continued to dominate in



August,







Wisconsin River



     Station one on the Wisconsin River showed shifts in species composition



between June and August.  In June, the algal population was dominated by the



blue-green alga, Aphanizomenon _flos-aquae,  the flagellated algae comprised



of Cryptomonas erosa_ and miscellaneous flagellates  and the centric diatom
               l™~™"~"™""""""                                                     /

                                    12

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Melosira spp.  In July, diverse algal population existed with no major




algal group showing complete dominance.  However, by August, the flagellated




algae had continued to increase, and along with the centric diatoms became




the dominant algal forms.  The flagellated algae were largely comprised of




Cryptomonas ovata, Chlamydomonas sp.,  and miscellaneous forms.  The




centric diatoms were represented largely by Melosira spp.




     Station two exhibited a population structure similar to Station one.




Throughout the study, the flagellated algae and centric diatoms dominated.




The species represented were similar to those present at Station one.




     Station three, during the study period, consistantly supported a




larger phytoplankton population than Stations one and two.  In terms of cell




concentration, phytoplankton numbers changed little between June and August.




In June, 9486 cells/ml were present, compared to 9510 cells/ml in July and




11,840 cells/ml in August.  Although cell concentration changed little




during this time periods species composition increased.  In June, the flagellates,




centric diatoms, and blue-green algae dominated.  Species represented were




the same as were present at Station one.  By July, the centric diatoms




became less important and the flagellates and blue-green algae dominated.




In August, the blue-green population decreased substantially and flagellates




and centric diatoms became numerically important.  The green algae also




began to increase in August being comprised predominantly of Crucogenia




.quadrata and Ankistrodesmus falcatus.




     It should be noted that throughout the study, green particulate matter




visible to the naked eye was suspended in the water of the Wisconsin River.




At all sampling stations, biological examination of the water in June and




July did not show any unusual increase in any of the phytoplankton species




encountered.  In August, this green particulate matter continued to "be




present, and due to heavy winds at the time of sampling, a large amount of



                                     13

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this material began to collect along the Eastern shoreline between




stations one and two.  Biologocal examination revealed the substance to




be the algal species Microcystis aeruginosa.   This species had reached




bloom conditions in August and the heavy winds were accumulating the




cells into a mat along the windward shoreline.









Chlorophyll




Fox River




     Station one showed a substantial increase in the amount of chlorophyll a_




present in the water compared to Station three during the months of June




and July (Table 10).  This difference was most noticeable in the June survey




when Station one had an average chlorophyll _a_ concentration of 47.5 yg/1,




compared to 9.13 yg/1 at Station three.  It should be noted that this difference




was probably due to the large number of phytoplankton cells observed suspended




in the water at Station one and in Swan Lake.  The algal population was




apparently approaching "bloom" conditions, although cell counts of water




samples did not show a "bloom" condition to be present.




     This large population of algae was not observed to  be present in July,




accounting for the significantly lower chlorophyll a_ values obtained at




Station one during July.  However, there still remained  a substantial




difference in the amount of algal biotaass produced at Station One (15.9 yg/1)




compared to Station three (6.72 yg/1).




     In August however, unlike the previous two months,  Station three showed




a substantial increase (25.1 yg/1) of chlorophyll £ present compared to that




at Station one (9.0 yg/1).

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Wis cons in Riyer_

     Station  three  throughout  the study period contained a greater amount

of algal biomass  compared to Station one.  The most significant difference

occurred in June  when Station  three had an average chlorophyll a_ value

of 23.1 ug/1, while Station one had a value of 13.4 yg/1.  This trend

continued during  July and August, however, the differences between the two

stations was  not  as pronounced.  In July, Station one had an average chlorophyll
                I
value of 10.4 yg/1, while Station three had a concentration of 13.3 yg/1.  In

August, Station one had a value of 22.0 Mg/1, while Station three had 27.7 yg/1

of chlorophyll a_  present.



Periphyton

FoxRiver

     Examination  of the periphyton community in July showed Station one on

the Fox River to  be more productive than Station two further downstream.

As one can see from Tables 11 and 12, Station one supported 23,298 cells/mm2

compared to only  5978 cells/mm2 at Station two.  Unfortunately, the periphyto-

meter at Station  three was  either lost or stolen, thus no comparison between

the other stations could be made.

     With regard  to species'composition, Station one was dominated by the

blue-green algae, represented by 0 s cilia tori a s p. and Coelosphaerium

kujatzingianum  and the pennate diatom Cocconeis pediculus.  Station two was

dominated by _0sicillajtpjria_ £p_. and Cocconeis pediculus.



Wisconsin River

     The periphyton community on the Wisconsin River showed Station one

to be less productive than either stations two or three.  Tables 11 and 12

shows that in July, Station one supported 221 cells/mm2, Station two


                                    15

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4928 cells/mm2 and Station three an average of 49.48 cells/mm2.


     Species composition showed Station one to be dominated by the centric


diatom Melosira spp.  Station two was dominated by OscillatorjLa sp.,  and


Cocconeis pediculus.  Station three was beet represented by the blue-green


algal species Aph an i z omen on flos-aquae and Oscillatoria sp., and by the


pennate diatom Cocconeis pediculus.




Macroinvertebrates

Fox River


     The results of the qualitative and quantitative macroinvertebrate


samples are contained in Table 13. Station one data showed a great diversity

                                                                           0^
of organisms in the qualitative samples for all months sampled (June  - 24 taxa,


July 24 taxa, August - 37 taxa).  For the most part, these organisms  were


associated with the stream margins, in and among the rooted vegetation.

     Quantitative samples indicated habitation of the bottom sediments by


a highly diverse population in early summer (31 different taxa in June) and


moderate diversity in July and Augusts with 19 and 20 taxa respectively.


     The population at Station one showed a mixture of pollution tolerant,


facultative and intolerant forms.


     Station two had a high diveristy in the qualitative sample for July


(43 taxa) and a lower diversity in August (12 taxa),  Ponar samples showed


a decrease in diversity as the summer progressed.  This was most likely


due to the decrease in dissolved oxygen and an increase in water temperature.


Also, the intolerant forms were not found in the August samples, as they

had been in June and July.
                                    16

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     Taxa diverlsty decreased at Station three during the summer months.

The quantitative samples had 45 taxa in June, 20 taxa in July, and 15 taxa

in August.  Here again, only the tolerant organisms were encountered at the

end of  the summer.  The qualitative samples had a high diversity of organisms

collected from among the abundant aquatic vegetation and along the margins

of the  stream.  The pollution intolerant forms found thorughout the summer

were for the most part, those organisms which live at the water - air

interface, and on the vegetation near the surface of the water (i.e., -

Helisoma limpsa and Amnicola Integra),


Wisconsin River

     Table 13 contains the qualitative and quantitative macroinvertebrate

data collected for the Wisconsin River during June, July and August.  The

quantitative data for all stations, for all months, exhibited low diversity

and low total numbers of individuals.  The moderately fast river current

and substrate composition (a mixture of fine to coarse sand) are-responsible

for the reduction of the organisms, since such conditions greatly inhibit

perminant colonization of the bottom sediment by the macroinvertebrate groups.

     The extensive colonization of the artificial substrate periphyton

samples and heavy utilization of overhanging trees and submerged vegetation

by organisms indicates that where the shifting sand substrate can be avoided,

a diverse population of individuals (as high as 34 taxa at Station three in

July) can develop.  The decrease in the number of taxa for the month of

August  on the Wisconsin River is probably due to the drastic drop in the

water levels  which exposed large sections of previously inhabited areas

of the river bed.
               !
                                     17

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     The macroinvertebrate populations at all'1 stations were a mixture of




pollution  tolerant, facultative, and intolerant forms.  This existed through-




out  the summer.









                               DISCUSSION




Station 1




     When  comparing Station one on'the Fox River to that on the Wisconsin




River, it  appears that during the study period, the Fox River is biologically




more productive than the Wisconsin River.  This is supported by the fact




that during the entire study period, phytoplankton cell concentrations were




substantionally greater in the Fox River than the Wisconsin River.  In June,




the Fox River supported 9741 cells/ml, in July, 14,300 cells/ml and in August,




14,910 cells/ml.  This compares to Station one on the Wisconsin River, which




in June, produced only 7344 cells/ml, in July 5680 cells/ml and in August,




8679 cells/ml.  Examination of the periphyton community present during the




July study, further supports the fact that Station one on the Fox River is




more productive than that on the Wisconsin River.  The Fox River supported




23,298 cells/mm2 compared with only 221 cells/mm2 on the Wisconsin River.




     Except for August, chlorophyll a_ concentrations showed greater




productivity in the Fox River.  Zooplankton populations (except in June)




were also  considerably greater in the Fox River.




     It should be noted that in July and August, several species of rotifers




were present at Station one on both the Fox and Wisconsin Rivers', which are




indicators of eutrophic conditions.  Although these species were present in




relatively low numbers, their presence may indicate that these stations are




approaching eutrophic conditions.  The species represented were Brachionus




£ngvjlaris_, Keratella cochlearis, P_. tecta, Trichocerca multicrinis, Filinia^





longiseta  and Pompjiolyjc sulcata.





                                    18

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     In terms of macroinvertebrate findings, Station one qualitative samples




from the Wisconsin River had a lower diversity of taxa .(15) as compared to




the Fox River (2?) .  Quantitative substrate samples also showed the Fox




River at Station one to be more productive than the Wisconsin River for the




same station.  Both rivers at this location had benthic communities composed




of tolerant, facultative and intolerant forms, which was characteristic




throughout the summer.




     Based upon the biological parameters measured at Station one, the Fox




River was classified as mesotrphic to eutrophic in nature, while the




Wisconsin River was classified as being mesotrophic.









Station 2




     Station two on he Fox and Wisconsin Rivers, for most of the summer,




appear to be similar in terms of phytoplankton productivity.  In June and




July, both rivers supported approximatley the same concentration of




phytoplankton.  The Fox River supported 5253 cells/ml in June and 2940




cells/ml in July compared to the Wisconsin River which supported 6018 cells/ml




in June and 3830 cells/ml in July.  In August, however, the Wisconsin River




supported a considerably greater phytoplankton population (9560 cells/ml)




compared to that in the Fox River (2729 cells/ml).




     A similarity, during June and July, between Station two on the Fox




and Wisconsin Rivers is further supported by examination of the periphyton




population.  Both stations supported approximately equal numbers of organisms.




The Wisconsin River supported 4928 cells/mm2 compared to 5978 cells/mm2 on




the Fox River.




     Fewer macroinertebrate taxa were encountered on the Fox River, Station




two (average of 23 taxa in the qualitative samples) as compared to the same




station on the Wisconsin River (29 taxa in the qualitative samples).  As the




                                    19

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summer progressed, the biological quality of the benthis community on the




Fox River at Station two deteriorated toward a greater number of pollution




tolerant forms, while the Wisconsin River remained relatively constant.




     The overall condition of Station two was considered to be eutrophic




to mesotrophic for the Fox River and mesotrophic for the Wisconsin River.









Station 3




     In June and July, the Wisconsin River supported a considerably greater




pollution enriched biological community than the Fox River.  This is




substantiated by the fact that the phytoplankton population in the Wisconsin




River in June reached 9485 cells/ml compared to only 2907 cells/ml in the




Fox River.   Chlorophyll j_ concentrations in the Wisconsin River (23.1 pg/1)




and the Fox River (9.1 ug/1) also supported this evidence.   The Wisconsin




River also supported a much greater zooplankton population (98 org/1) than




the Fox River (19 org/1).  In July, the phytoplankton and chlorophyll ji




also showed greater biological productivity in the Wisconsin River, while




both rivers supported similar zooplankton populations.
                                    20

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Table 1   Chemical and physical analysis of water collected  from the
          Fox and Wisconsin Rivers  June,  July and August, 1978.
£
M
to

>^
^
iH
3
•->
>>
Q)
>
H
0
to
4-1
Cfl
3
60
3
Parameter '
Temp. °C
Dissolved
Oxygen mg/1
pH
Specific
conductance
uohms/cm
Temp. °C
Dissolved
Oxygen
(mg/D
PH
Specific
conductance
(jj ohms /cm1)
Temp. °C
Dissolved
oxygen
mg/1
PH
Specific
conductance
y ohms /cm
Fox River
Station
1 ' 2 ' 3
23.5
12.2-14.4
8.9
560
23.5
8.4
8.0
360
23.9
5.9
—
400
17.0
7.0
7.8
. 600
21.5-22.0
3.7-3.8
6.9
400
21.0
2.7
__
400
16.0
2.2
7.6
600
20.5
2.2
8.25
400
23.4
3.90
7.25
400
i












Wisconsin River
Station
1 • 2 ' 3
17.0
8.4
7.4
160
23.0-24.0
7.45-9.6
7.6
140-156
26-26.3
8.75-8.80
7.7-8.0
150
21.0
8.4
7.4
160
20.5-21.0
6.8-7.4
7.5-7.55
150-160
24-24.5
7.6-8.3
7.5-7.75
150
17.0
8.4
7.4
160
21.0-22.0
6.6-7.5
7.1-7.5
160
23.0
7.05-7.3
—
160

-------
Table 2    Length and weight measurements of fish captured In the
           Wisconsin River during August Survey 1978.
Station 1
Species
 Number
Captured
    Weight
(range in 02.)
    Length
(rangein inches)
Carpoides cyprinus
(Quilback carpsucker)
Minnows
1
3
64.0
„_
16.5
1.0-2.5
Station 2
Species
 Number
    Weight            Length
Carpoides cyprinus
(Quilback carpsucker)
Micropterus dolomeiui
(Smallmouth bass)
Minnows
Perca flavescens
yellow perch
1
1
1
1
64.0
16.0
—
8.0
16.0
9.5
3.0
6.75
Station 3
Species
 Number           Weight            Length
Captured	(range In oz.)	(range in inches)
Carpoides cyprinus
(Quilback carpsucker)
Micropterus salmoides
(Largemouth bass)
Moxostomo anisurum
(Silver redhorse)
Minnows
2
1
1
5
7.0
9.5
40.0
—
5.5-5.75
8.5
15.5
2.0-3.25

-------
Table 3    Length and weight measurements of fish-captured In the
           Fox RJ.ver during August Survey 1978.
Station 1 *
Species
 Number
Captured
     Weight
(range in oz.)
   length
(range in inches)
Am la calva j
(Bow fin)
i
Leporais cyanellus
(Green sunfish)
Ictalurus nebulosus
(Brown bullhead)
Lepomis microlophus
(Redear sunfish)
Perca flavescens
(yellow perch)
Sunfish
(young of the year)
1
1
1
A
1
15
9.0
2.0
9.0
—

„_
7.5
3.5
7.5
2.75-3.50

—
   *  Due to a malfunctioning of the Electroshocker,  no fish samples
     were taken at Stations 2 and 3.

-------
Table
Fish species collected by electroshocking on the Fox
and Wisconsin Rivers during August 1978 survey.
Species Fox River
Station
123
Amia calva
(Bowfin)
Carpoides cyprinus
(Quilback carpsucker)
Lepomis cyanellus
(Green sunfish) !
Lepomis microlophus
(Redear sunfish)
Micropterus dolomieui
(Smallmouth bass)
Micropterus salmoides
(Largemouth bass)
Moxostotna anisurum
(Silver redhorse)
Ictalurus nebulosus
(Brown bullhead)
Perca flavescens
(Yellow perch)
Stizostedion vitreum
(Walleye)
Minnows
(Unidentifed)
Sunfish
(Young of the year)
X

X
X



X
X


X

N
0

s
A
M
P
L
E




N
0

S
A
H
P
L
E



Wisconsin River
Station
12 3

X








X


X


X


•

X
X


X



X
X



X


-------
Table 5 Zooplankton in organisms/
Rivers in June, July and i
June Survey
Fox R. Wise. R.
Station No.
Species \ 1 313
Rotifera
Asplanchna priodonta
Brachionus angularis
Chromogaster ovalis
Colurella sp.
Conochilus unicornis
Euchlanis sp.
Filinia longiseta
Kellicottia bostonensis
Kellieottia longispina
Keratella cochlearis
Keratella cochlearis
v -tecta
Keratella crassa
Keratella carlinae
Monostyla lunaris
Monostyla quadndentata
Monostyla sp.
Polyarthra major
Polyarthra romata
Polyarthra vulgaris
Polyarthra sp.
Pompholyx sulcata
Synchaeta sp.
Trichocerca multicrinis
Trichocerca rousseleti
Trichocerca similis
Trichocerca sp.
Trichotria tetractis
Unid. spp.
OG
CLADOCERA
Clydorus sphaericus
Eubosmina coregoni
COPEPODA
Cyclops juveniles
Cyclops sp.
Diaptomus juveniles
Nauplii










5




















2




2
2






8



2




2
2





2





2


















2
36





i _

7



81

8












3










27





2

5



53

2







2




5
L collected from the Fox
\ugust 1978.
July Survey
Fox R. Wise. R.
Station No.
1313



2
8
2
2



75


23




8
19

3
5






6




3


5





2
2



6



3




3







3
6








9





2


2

14
2
2
3
2



2
8


5



5

2
2

3





6




2


2


6


2
2



2
3


8














5
and Wisconsin
August Survey
Fox R. Wise. R.
Station No.
1 3 1-3

2

5

f

2


105

6
9



6



2

5


19

5








22






2 __,











3



5











2
2

3


2







12
2




2





2
2
2



2








2 1


2







16



2

2

2




















-------
Table 6   Major zooplankton groups collected from the Fox and
          Wisconsin Rivers in June, July and August 1978.'
>>

%
£
3
LO
£
D
•-i
>.
Q)
I
D
CO
4J
W
3
60
D

-------
Table 7        Phytoplankton in cells/ml collected from the Fox and Wisconsin Rivers
               in June, July and August 1978.
June Survey
Fox Rivor Uisc
Station No. Statj
Epc-rie". ' 1 ' 2 ' 3 ' 1 ' 2a
BLUE-GREEN ALGAE
Agroenellutn sp.
Anabaena sp.
Anacystis cynea
Anacystis sp.
Aphanizomenon flos-aquae
Chroococcus sp.

Coelosohaerium
Coelospharium sp.
Lyngbva martensiana
Microcystis aerueinosa
Oscillatoria limnctica
Oscillatoria sp.
GREE3 ALGAE
Actinastrium hantzschii
Actinastrlum sp.
Ankistrodesmus falcatus

Closterium sp.
Coelastrum microporum
Coelastrmn sp.
Cosmarium sp.
Crucigenia quadrats
Crucigenia tetrapedia
Crucigenia sp.
Dictyosphaerium pulchellun
Dictyosphaerium sp.
Golenkinia sp.
Klrchneriella sp.
Micraccinium sp.
Oocystis sp.
Pediastrum boryanum
Quadrigula lacustris

5610


3315





























51


204







153
























51











204


51




102
51





51

51
sT"


2142





51





204







102






51


102


510







51



153














153

River
ton No.
2c -

51


714







51



153


51




102
51





102

3

51


1938



.



51

51

357



51










153

July Survi.y
fox Kivcr Wise, River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3

90
290

5850
90

90




90


















30



90

260


60




110





30






30

60


90




30

140
90

110




230



110





170



30
30


110
30


30
110
30
520


90








490





350
60







30



200

290
30






30


30
170







140




60





200

170







30



260





170








30


30
230

3160
140

30












110
30

230





30
30


August Survey
Fox RJver Wise. Rivtr
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3

70


830


30

30

30


















140




30

100







100

















70



1100
280

280







210


210
1040



70

140







350



240
30
210

70











410





210


30



30
70



100
30
720

100


70




210



140





140





100

30
30







N
0








s
A
M
P
L
E












240
450

30


30



100



70
550



30

720







30



-------
Table 7

cont'd
Phytoplankton in cells/nil collected from the Fox and Wisconsin Rivera
in June, July and August 1978.
Soecies
Scenedesmus abundans
Scenedesmus bljuga
Scenedesmus quadricauda
Scenedesmus dimorphus
Scenedesmus sp.
Schroederia setigera
Selenastrum sp.
Tetraedron caudatum
Tetraedron minimum
Tetraedron sp.
Tetrastrum staurogen-
laerorfie
Tetrastrum sp.
Misc. greens
FLAGELLATES
Ceratium hirundinella
Chlamydotnorus sp.
Cryptomonas erosa
Cryptomonas ovata
Cryptomonas sp.
•Eudorina sp.
Euglena sp.
Mallomonas sp.
Pandorina sp.
Phacus sp.
Trachelomonas sp.
Misc. flagellates
CENTRIC DIATOMS
Cyclotella sp.
Melosira sp.
Microsiphona po tamos
Stephanodiscus sp.
PENNATE DIATOMS
^Asterfonella fonnosa
June Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ! 1 ' 2a ' 2c •' 3 '














51

51

408






102









51












51
102

51

51



51
612

3570






51
153








51




306

153
-




51^
510

459
306




153

357













765





51


714

102
2244

51




153

51

153









663

403






510


2295

51




153

153











459

408


51



612

204
2091

51

408


204
51





51

102




459

765






1632

612
1683

102

816
July Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3





90











90
820


60



1940

30
430









30











60
410

30
30



1300

60
60






60


30

30

60





60
110
230
430

30
30
30
30

2260

110
1160




60

30

30
110
30








30
170
230
290


30

30

1480

30
1040
90
30


60




60
60

30



110



90
350
290






1100

110
670

60


30

60

30
110



30






90
350
460




60

750

110
670
30





60

60
230











350
350






3010

90
1100
30



August Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a 2c ' 3


30


30








280

70
70
240

30
30



12250



70














30


30




1860

30 | 100
140




30

30




70

70
30


70

70




410

140
350

30


30
30
4140

550
550
690





30

70
30



30
100




410

410



30


30
2760

280
2760
100
100




70

140




3D






30
70
140

60
30



3100

310
2860
790
30



































170

140
70




30




270
410
310
210
30
30
70

30

2970

1000
1690
1860

-



-------
Table  7
cont'd
Phytoplankton in cells/ml collected from the Fox and Wisconsin Rivers
in June, July and August 1978,
June Survey
Fox River Wise. River
Station No. Station No.
S^£c-_6S ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c .
Cocconeis sp.
Cymbeila sp.
Diatona sp.
Fragilaria crotonensis
Fragilaria construens
Gomphonema sp .
Kavicula sp.
Nitzschia acicularis
Nitzschia palea
Nitzschia sp.
Synedra acus
Synedra ulna
Synedra sp.
51







51
102




51





,
204
51



102



204




51











153
102







1020




51











102
i



3








204
153



July Survey
Fox River ULSC. River
Station No. Station No.
1 ' 2 ', 3 ' 1 ' 2a ' 2c ' 3
30




30
30






30





30

110
90



110


30


60
30
60


90









30












30
30
30

















30



30


90



August Survey
Fox River Wise. River
Station No. Station No.
1 ' 2 ' 3 ' 1 ' 2a ' 2c ' 3
30


310


100






30






30
210




70


210


70

70 '
210





30




30

140
30










100
100


30



















140
240
70

30
30


-------
Table 8     Major phytoplankton groups collected from the Fox and
            Wisconsin Rivers in June, July and August 1978.
£
fc
3
tn
0)
§
>->

r
M
to
^
,H
*->

August Survey
Fox River
NO/ral
Station No.
Species 123
Blue-green algae
Green algae
Flagellates
Centric diatoms
Pennate diatoms
Total

Blue-green algae
Green algae
Flagellates
Centric diatoms
Pennate diatoms
Total

Blue-green algae
Green algae
Flagellates
Centric Diatoms
Pennate diatoms
Total
8925

612
__
204
9741

7250
140
5860
870
180
14300

1130
200
12970
170
440
14910
408
51
918
3570
306
5253

520
210
1830
120
260
2940

230
140
1920
160
270
2720
51
714
1020
765
357
2907

710
660
3210
1270
380
6230

1940
2050
5160
1790
630
10940





















Wisconsin River
NO/ml
Statiog No.
1 2a 2c 3
2295
867
1530
2397
255
7344

1010
1190
2260
1190
30
5680

550
1010
3640
3240
230
8670
663
663
1581
2346
1071
6324

660
860
1830
840
90
4280

1230
680
3430
3990
230
9560
867
765
1530
2346
510
6018

490
820
1710
810
—
3830

8
s
A
M
P
L
E

2040
1020
2856
2397
1173
9486

3650
780
3710
1220
150
9510

850
1810
4120
4550
510
11840
*  The letters A and C following the station number are used to denote
   substations which were taken along a transect across the river channel
   at that station.

-------
table 9     Diatom species proportional count by percent  of phytoplankton collected from
            Che Fox and Wisconsin Sivers in June,  July  and August 1978.
June Survey
Fox River Uisc. River
Station No. Station No.
!--2ci-.3 ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c •' 3
Achnathes sp.
Amphora sp.
Asterionella formosa
Cocconeis pediculus
Cocconeis placentula
Cocconeis sp.
Cyclotella comta
Cyclotella glofaerata
Cyclotella meneghiniand
Cyclotella stelligera
Cyclotella sp.
Cymbella sp.
Diatoma tenua
v.elongatrura
Diatoma vulgare
Diatoma sp.
Diploneis sp.
Epithemia sp .
Fragilaria capucina
Fragilaria crotonensis
Fragilaria construens
Fragilaria intermedia
Fragilaria leptostauron
Fragilaria pinnata
Fragilaria sp.
Gomphonema olivaceum
Gomphonema sp .
Melosira atnbigua
Meloslra diatana
Halosira granulata
Melosira longispina
Malosira islandica

2



6


3

25
2











42












2


90

2
<1






1




2

<1










16


20





3








15



27





11


<1


1
<1

<1
<1

2



1




3



4



<1
<1
18


<1


<1


<1


2



1




10



5





15


<1


<1


<1


<1

<1

3




2







<1

10





<1
<1




2








2



1



July Survey
Fox River WI.sc. River
Station No. Station No.
1 ' 2 ' 3 ', 1 ' 2a ' 2c ' 3



6


<1



<1
5




2

20

<1


2

5
43

5


1

<1
34
3



9

<1
<1


5



3
13




<1
<1
4






<1
17
<1



13

3
<1


3


3
3
4
6




<1
12
2



2


<1
<1



<1

<1
<1
<1

<1



i
2



2

<1
37
1



2

1
1




<1
1
1



1

<1

5
5



2

1
49
4

2

<1

2
<1
<1




<1
1
<1
2
<1
<1




4



9


38
5



2

2
<1
<1


1
1
<1
7
<1
3

2
<1
<1


<1

<1



2
40
7
1


Auguat Survey
Fox River Wise. River
Station No. Station So.
i i ' 2 ' 3 ' 1 ' 2a ! 2c ' 3



1




<1

<1
<1




<1

93




<1







2


19
4



4

38
<6




2






16







1
<1

15
<1



7

7
<1
1






4



10

<1
4
1
3




2





2










3






7
13
8

3
<1

3
3




<1

3
<1
<1













24
7
1

































<1


1
<1



2

<1

1





1
8


<1
3


11
22
4

5

-------
Table 9
continued
Juna Survey
Fox River Wise. River
Station No. Station No.
Soecies ' 1 ' 2 ' 3 ' 1 ' 2a ' 2c - ' 3
Meloslra Italics
Hclosira varians
Melosira sp.
Navicula sp.
Neidium sp.
Nitzschia acicularis
Niczschia holsatica
Nitzschia palea'
Nitzschia sp.
Pinnularia sp.
Khoicosphenia curvata
Stauroneis sp.
Stephanodiscus nlagarae
Stephanodiscus sp.
Surirella sp.
Synedra acus
Synedra ulna
Synedra sp.
Tabellaria fenestrata
Thallosira fluviatllie


13 •
2
2



2



4










3

<1










<1




11
3
3

<1






<1



2




<1
70
3

<1


1



1

<1






2
54
2

<1
10


-------
Table 10    Chlorophyll a^ concentrations of water collected from the
            Fox and Wisconsin Rivers in June, July and August  1978.
                      Chlorophyll a_ (corrected for phaeophytin)
                                         ug/1
June
Survey

July
Survey

August
Survey
River
Fox River
Wisconsin R.

Fox River
Wisconsin R.

Fox River
Wisconsin R. •
* la '
48.8
13.9

16. 40
10.92

0.4
18.90
Ib '
65.9
13.0

15.63
9.85

9.6
25.53
Ic '
28.0
13.2

15.58
10.54

9.0
21.60
3a (
9.0
23.8

7.20
12.80

19.8
26.2
3b 1
9.6
19.8

5.19
13.67

33.2
27.8
3c
8.8
25.6

7.76
13.37

22.4
28.95
*  The letters a,  b and c following the station numbers are used to
   denote substations which were taken along a transect across the
   river channel at that station.

-------
Table 11       Periphyton in cells/mm2 collected from the Fox and
               Wisconsin Rivers July 1978
Wisconsin River
Station
Species 1 ' 2 ' 3a ' 3c
Blue-Green algae
Agmenellum sp.
Anabaena sp.
Aphanizomenon flos-aquae
Coelosphaerium kuetzingianum
Oscillatoria sp.
Green Algae
Actinastrum sp.
Ankistrodesmus falcatus
Closteriura sp.
Pediastrum boryanum
Scenedesmus dimorphus
Scenedesmus falcatus
Scenedesmus quadricauda
Scenedesmus sp.
Stigeoclonium glomerata
Flagellates
Chrysococcus sp.
Cryptomonas oyata
Chlamydomonas sp.
Cryptomonas erosa
Mallomonas sp.
Trachelomonas sp.
Misc. flagellates
Centric Diatoms
Cyclotella sp.
Melosira sp .
Pennate Diatoms
Asterionella formosa
Cocconeis sp.
Cymbella sp.
Gomphonema sp.
Navicula sp.
Nitzschia sp .
Snyedra ulna













5
3








31

5
122

8


3
34
10



24


3000


24
2

9
3
16
19


2





67


176


1380


57
86


25

1530

4300







3









12


60


628
21
521
18
34






86

<1
<1

<1








<1
9

<1
4


5


1304

1
2
3

Fox River
Station
1 1 ' 2 ' 3




2800
5950


14






70


28




218


252


13594

14
15
126





40
5450


<1


3

3





3
1
<1

94

4
31


336

<1
5
8
<1


N
0






s
A
M
P
L
E




















-------
Table 12   Diatom Species proportional count by percent of periphyton
           collected from the Fox and Wisconsin Rivers In July 1978.

                                     July Survey
                            Fox  River          Wisconsin River
                            .  Station              Station
Species
Achnathes lanceolata
Achnathes sp.
Amphora sp .
Anomoeoneis sphaerophora
Asterienella formosa
Cocconeis pediculus
Cocconeis placentula
Cyclotella glomerata
Cyclotella meneghiana
Cyclotella sp.
Cymbella sp.
Diatoma tenue v. elongatum
Dia t oma s p ,
Epitliemia sp.
Eunotia sp-
Fragilaria construens
Fragllaria crotonensis
Fragilaria intermedia
Fragilaria sp.
Gomphonema olivaceum
Gomphonema sp.
Melosira ambigua
Melosira distans
Helosira italica
Melosira varians
Melosira sp.
Navicula sp .
Nitzschia acicularis
Nitzschia palea
Nitzschia sp.
Pinnularia sp.
Stephanodiscus binderanus
Stephanodiscus niagarae
Stephanodiscus sp.
Surirella angustata
Surirella sp.
jiynedra ulna
Sjynedra sp.
Tabellarla fenestrata
Tabellaria fenestrata
V. geniculata
Thallosira fluviatilis
1



<1

98
<1



<1




<1
<1


<1






<1














2

<1



74
<1

<1
3
<1

<1


3

1

1




It

6
<1
<1
1


1
1



1



3



N
0



S
A
M
P
L
E



























f 1
1
<1



79
1


1


1


3

1



3
1
4


2


<1


<1




2



2a



N
0



S
A
M
P
L
E



























2c

<1


<1
77
<1

<1
3
<1
<1
<1
<1

1
<1
3

<1

<1
2
3
<1
3
1


<1

<1
<1




<1
<1
<1
<1
3
<1
<1



96
<1


<1



<1
<1



<1
<1

<1

<1
<1
<1
<1


<1
<1




<1

<1




-------
Dlptera
                                 June
                             I'     2
                             labie  U
  Macroinvertebrate  Data  For  the Fox  River, Pottage, Wisconsin
Qualitative  Sumplce                                         Quantititlva  Samjilcsi
    July              AuuuHt                June                 July
123123           1231231
August
   2
                                                                                                                                                       Tolerance
Ablabesnyla ap.
Oratopoponldae
Chironoaus sp.
Cllnotanypua sp.
Crlcotopua sp
Cryptochironomus cp
Diamesa sp
Dlcrotendipes ep
Eadochlronomus sp
Eukiefferiella ep.
Clyptotendlpes sp
Goeldlchlronomus sp
Harniscnia sp.
Klefferulus sp.
Mlcropsectra sp
MIcrotendlpes sp.
Parachlronomus sp
Paratendlpes sp
Pentaaeurlni tribe
Phaenopsectra sp
Polypedllun sp


1
1

3
1

19
37

31

1



8
a


5

/
/
/
/
/ '
/
/
/
/
/
c
0)
^
H
m
ex
e
a
o
z
/
/
/
/
/
/
/





2


6
1

6





1

2
14
3









80

30





4







1




1
14

1





3

7

1

















1






32





12
132










204






1



3











1








1
1









2

13
























8
20
4

4

148
30

16

26
6
10

4
4
2

112



27

18

1
3
2







12

1

1

2

1

21


14
4

11





2
2

17
9


8
12


60

28


476
4

8










77


13


1

2

2



8

4

9





29





4





9



20


2



11

11
6

2







6







1
4

1































4

2
i










































i
I
F
T
F
F

I
F
F

T




I
F


I-F
F
























-------
            Ijblc 1J cunc'd

              Macroinvertebrnte Data For the Fox River, Portage, Wisconsin
                                                                                                         rage
            Qualitative Samplo
June            July               August
231231      23
             Quantitative Sample
June               July               August
 231       2      31       2
                                                                                                                     Tolerance
Dlpteira
Procladiua sp
Psectrocladius sp
Pseuiochiroaomus ap
Sinullidaa family
Sicmliidae pupae
Tanypus sp
Tanycarsinl tribe
Thienemanniella ep.
_Tipulldae family
Tribelos sp.
Trichocladius sp
Unidentified pupae
Unidentified sp









1





172














/
/
/
/
/
G
O
- --5
H
ta
£X
1
o
X
1
1
1
1
1










1




8




1
1















2




10










3




1
25




2













1


















12





20




8






































2




2






























I

116





942




46
20









40
1



2



1


1









1
1




10




1
1









34





192



8
20
1









6





7

















4



















29





175




3










4


















__


































































*

T-F
F
F-I
F

F






































-------
       Table  13  cont'd
                                                                                           Page 3
         Qualitative Sampled
   June             July              August
1231231      23
       QuanLlLaLJvc Samplca

 June                 July
12312
        August
3     1      2
                                                                                                                         Tolerance
TRICHOPTERA
A^rijlpa flp.
Coraclca up.
Cheioato psyche sp.
Cheumatopsyche ep. (pupat
Kydropsychldae
Hydropsyche sp.
Hydropsyche sp. (pupae)
Hydropclla sp.
Hydropttlidae
Leptocerldae
Lepcocerus sp.
Lypa sp.
Mlcrosema sp.
Nectopsyche ep.
Neureclipsis sp.
Oecetis sp.
Pslyccntropidae
Sericoscoma sp.
theliopsyche sp.
Ualdeccifled sp.

1


)





















"-

*







•















1





1









4










3



1















1
3



























1









2

1

4

1































3







1


























3














f>



































4
1














































10







4

































2












1
1





















































































































-------
labla 13 conc'd
                                        Fox
                                                                                 Page 4
June July August June July Augusc
1231231 23 1 231 2 31 2 3 T1
ODONATA
Apurlinn up.
Anaz sp.
Coenagrlonidae
Ischnura op.
Libellulldac
Nehalennla Irena
Perltheous donltla
Tecragoneuria op.
Zygoptera
Unidentified op.
EPHEMEROPTERA
Baetldae
Caenls sp.
Cencropcllua sp.
Ephoron sp.
Hepcagenlloae
Hexagenia llnbala
Hexagenla sp.
Neoepheraera blcolor
Slphloourus sp.




1



1


















- -
i.

























1














1
1
1





















10







































2


64



5




8







1




3





















9





1








2



























































3











1









2







































1









2












1



















2
















1
1




































1 1

























































































-------
Jutia
  2
Table 13 conc'd

          Quantitative  Samples

            July
        123]
                                          Fox
August
 2    3
                                                                                                         Page i
June
 2
Quantitative  Samples

  July                August
    2312
EPHEHEROPTERA
BtFiuinrnm up
Unidentified e?

COLEOPTERA
Berosus sp
Coptotaaus sp
Dineutus sp
Dublraphia sp.
Enochrue sp
G\rinldae
Cyrinus sp
Hallplus sp
Hydrophilidae
Uydroporlnae sp
Laccophllus ep
Peltodytea sp
Daldentlfled »P






























- -













































1
2



























1
1
1




1







1








5
















3








1







30




1










1




12

1

A3



1
7

























1











2


















1













































































































3


































































































1



































!


i 1




-------
Toblo 13 conc'd
                            Fox
                                                                                        F«g« 6
Qualitative Quantitative
Juno July Auguut June July August
123123123 123123123 Tnl(1
HEMIPTERA
Abedua sp
Corlxldae
Lethocerus sp
Hesovellldaq
Ha tooec ta ap
Pelocorla sp.
Plea ctriola
Ranatra sp.
Sigara sp.
LEPIDOPTERA
Pyralididae

COLLEMB01A
Isocoaa sp.
Isotomurus paluscris
































- -









































a




















4
1


2
6







































1


3

2
5


4


































3











































































































n



























































































i






























i




























.
ranee;




































































-------
Table 13  conc'd
                                                                                          Pjgc  7
Qualitative Suni|ilon Quant Untlvn Snmplcu
June July Augual . Juno July August
Species 123123123 123123123 T«i., „.,..„
ISOPODA
f*i? \ luq up ,
A. racovltzal
CLADOCEFA
Alooa sp.
Daphnla galeata
D. juveniles
D. 'pule*
D. ap.
Cubosniina coregonl
Eurycercus lamellatus
Lepcadora kindcil
Sida crystalline
Sinocephalua serrulat
0nlder.tif led ap.

































- r-










-


5







2

2
e


1


















6





















14


2


18









3





1





12















2





































47














































2


















6


5


















19











3











A



40





















10





4









16











3





















6

















































!



-














































































!





-------
            Table 13 conc'd
                                                                                        Page 3
               (Junntl tncJvi> SnmplrH
  June                July
1231231
June
                                           23
fjualicnclvo  Sfi
         July
312
                                         Auguat
                                     123
                                                                                                                           Tolerance
AMPHIFODA
Cnrrviruit up.
Hyalella op.
COPEPODA
Copepoda
Cyclopolda
Cyclops blcuspldaeus
FELECYPODA
Musculinum lacustra
Sphaerildae
GASTROPODA
Amnlcola Integra
Annlcola llmosa
Amnlcola sp.
Campeloma decisa
Cyraulua hirsucua
Gyraulus parvus
HellBooa trivolvis





205













78




I




- -



















38






2

53




6
25






51













70







252








6



32
15
63






5


3





10





1






303













2




























39






3

1
53


2
6




j
























a

4


















14

36




2


2








I


21





4
2

95
2




13






56





















21


1









4

4



























70


















i


2










1

















3


































































































1

-------
1J cone a
         Qualitative Samples
  June              July         •    August
1231231       23
                                                    Quantitative Sam.ile
                                             June               July
                                           12312
  August
1      2
                                                                                                        Tolerance
GASTROPODA (cont'd)
Hydrnbia nicklinlana
Lyanaca sp.
Physa elliptic^
Physa Integra
Physldae
Flanorbldae
Pleurocera acuta
Pleurocera sp.
Proaenetus exacuoua
Valvata sp.
Valvata ccicarnata
Unidentified sp.
OTHER
BRYOZOA
TURBELLARIA.
Cura foremanli
COELEKTERAIA
Hydra sp.
Hydracarlna sp.
HQ1AIODA




13
*





1






4






- -




















14




2

2











9

27






2






1




1

23






3






5




a

3




















2





1



INC




1





















3




1



1
15

3
















































1

15

1


5




22

3






1

8




44






37




84

23

2




















76





2












4

46












5







21




















24




















14











2








6





















































































-------
                              Talbc 13 cont'd
                                                                                                Page  10
June
Quail till Ivu SilmplaH
      July              Auguut
         Qu.-mcltJllvu  Sum
June                July
                                                                                                     AugUHC
123123123 123123123To,
OTHER (cont'd)
>.THATOHOH?11A
Paragordius sp.
TCBBELLAIUA
Cura forcmanii

Total taxa





t













54

26



















- -

NS








•












33



















6

24








-










35

43



















8

22



















123

37



















1

12



















2

37



















•





















2

31



















74

31





















45



















36

19



















60

30



















124

20



















18

20



















24

17



















12

15





































prance



































































-------
Table 14
Qualitative and Quantitative Macroinvorcebrate Date Collected  from  the Wisconsin
Hiver during June, July and August, 1978 at Portage,  Wisconsin
              Qualitative Samples
                                                              Quantitative Samples
Month June July Augunt June July August
Station 123123123 123123123 Tolerance
DIPTERA Ablabesmyia
C^rat opo?,onld-ie
Chlronomus Bp.
Cllnotanypua sp.
Cricotopua ap.
Cryptochironoaua ep.
Diaraesa ap.
Dirotendlpes ap.
Endocbircaomus sp.
Eukiefferiella sp.
Glyptotendipes sp.
Coeldichironomus sp.
Harnlschla sp.
Kiefferulus sp .
Micropsectra sp.
Klcrotendipes sp.
Farachlronoous sp .
Paratendipes sp.
Pentaneurlnl tribe
Fhaecopsectra sp.
Folypedllua sp.

ap.

2

2


AS
2

82
1



2
9



34



a

-8 -



68

112




•
24



2



-

1

1
6
2







4


2
12







13



4









9



1


1

1
12
5

1




1



72





27



2
8






1



90





















i



































































7





















7



1













2
8


15
2

3















7


3











8










i



















18






1











1
9





















6





















33




















1










































j
I
F
T
F
F

I
F
F

T




I
F


F-I




















' i
i

-------
     Table 14
11.   coat'd
                 Qualitative Samples
         June               July
   Augus t
1      23
         Quantitative SnmpleB
   June               July
12312
                                                                   August
                                                                                                                                  Tolerance '
Dlptera (conc'd)
PITH Intlfnn op,
Psectrocladius sp.
Pseudochironosus &p.
SlDullidae family
Slmullldae pupae
Tanypus sp.
Tanytarsini tribe
Thienemanpiella sp.
Tipulidae family
Tribelos sp.
Trichocladlus sp.
Unidentified pupae
Unidentified sp.







'



1



6





9












-








4











1









1












147
7

3













!




i
2

5
1

1

1













385
3

12
4


1
Z
1















6













































1





1














1










1




























3

3



1












1








1










































4







1




1


























1





















1

























1





















































T-F
F
F-l
F

F



















i

















-------
Table 14 conc'd
12
            qualitative Saraplua
                                                                       Quantitative  Samplca
June July August June July August
123123123 123123123 Toler3nr,
TRICHOPTERA
Clit'umilop'JVrlH' up.
Che uma to psyche sp.(pt
Hydropsychldae
Hydropsyche ff.
Hydropsyche sp.Cpupai
Hydroptlla sp.
Hydroptilldae
Leptoceridae
Leptocerus sp.
Lype sp.
Mlcrasema sp.
Nectopsyche sp.
Seurecllpsis sp.
Oecetls sp.
Polycentropldae
Sericostoma sp.
Thellopsyche sp.
Unidentified sp.



20
pae)


)







2











- -




























8




l""1




420
27

70
3












1



198
4

25








1




1



147
20

S3


3

25



15


M




2









1



2


2
























3










1



















































1

5

2
















2























4






































2


3



























































4






























































T


F




F

































-------
Qualitative  Samples
Quantitative  Sniui>li-s
June
123
ODONATA
At*n( hn.l lip .
Anax sp.
Coenagrlonldae
Ischnura ep.
Llbellulldae
Nehalennla Irene
Ferlthemua domlcia
Tetragoneuria sp.
Zygopcera
Unldenclfed ep.
EFHEHEROPTE8A
Baetldae
Caenls sp.
Cencroptilum sp.
Ephoron sp.
Heptageniidae
Hexagenia limbala
Itexagenia sp.
Neoephemera bicolor
SlphlcHiuniQ ep «
















3




1




- -
'






1




1













1












July
1 2














26





















39



1



3










1



33







Aug
1






















U3C
2






















3






















June July August
123123123 ,„,_„„„



























































1




































































































































































































































1
? I

I






'








-------
Table 14  conc'd
June
1 2
EPKEMEROPTERA
St^non-M "P-
Unidentified sp.
COLEOPTERA
Berosus sp.
Copcotoaus sp.
Dlneutus sp.
Dablraphla sp.
Enoch rus sp.
Gyrinldae
Cyrlnus sp.
Haliplus sp.
Kydrophilidae
Hydroporinae sp.
Laccophilus sp.
Peltodytes sp.
Unidentifed sp.






1




















1

-2 -

1








-
1




QunliL.nL.lva Samp
July
312









2
2
1
1































1



3














3


1


















QuuntltaLivo ,'Inmplei
Auguet June July Auitust
1 2 ^ 1 2 3 1 2 3 1 2 3 Tn,_











































3



















































































































































2

































































































































F



T

T









I




















-------
       Table  14  conc'd
       fS

             qualitative  Samples
    June            July            August
1231231      23
        Quantitative Samples
   June              July
12312
       August
312
                                                                                                                         Tolerance
UEMIPTERA
Abr-dua Bp.
Corlxldac
Lethocerus sp.
Kesovellldae
Kotonecta op.
Pelocorla sp.
Plea striola
Ranatra sp.
Slgara sp.

LEPIDOPTERA
Pyralldtdae

COLLEMBOLA
Isotoma sp.
Isotoo&rus palustris





















1







7

-' -



















«5
1
1



















3




























65





















70






































































































1





















2




























5


























































































































































































!


F










































-------
Table 14   cont'd
         Qualitative Sampled
June             July
   23123
                                 August
                               1      23
           Quantitative Samples
  June                July              August
1      2     3     1     72      3     1  *   2      3
                                                                                                                      Tolerance
OLICOCHEATA
DtTll H|).
Limnodrllua sp.
Naldldae
Hals sp.
Peloscolex sp.
Stylaria lacuscrla
Unidentified
EIRUDINEA
Batracobdella paludo
B. phalera
Dlna micros toma
DIna partra
Eropbdellldae
Glossiphonla conplan
G. heterocllta
Belobdella elongate
H. fusca
U. llneaca
H. paplllata











ta




ita










61
- -


33


















9




















5




















18
1



1
















1
69



10






1
































































































1






















1

1

2


















1


1































































































































































































T

P






T
T

I






























-------
Table 14 cont'd
n
(JiiolltntLvo S.implca (Juaiuitutlva Samples
June July August June ' July August
123123123 123123123 Tolerance
U. Btagnalls
II. op.
Illlnobdclla sp.
Plsclcolldae
Placcbdella ornata
UnldentlEed op.








































. .










•
































































































































































































































































1








































































































1








































T










































-------
Qualitative Lamp lea
                                                         Quantitative Samplers

ISOFODA
A »
-------
Table 14 cont'd
19

AMPfilFODA
CincnariiFi Hp.
Hyalella azceca
COPEPODA
Cyclopolda
Cyclops blcuspidctus
PELECYPODA
Huacullum lacuscre
Sphaerlldae
GASTROPODA
Amnicola Integra
Amnlcola llQosa
Acnlcola sp.
Canpeloma declsa
Cyraulus hlrsucus
Gyraulus parvus
Hellsoma crlvolvls





June
1 2

3






















&

- -


*






2







Qualitative Samplea Quantitative Sumplea
July August June July August
31/31 2 3 1 2 3 1 X2 3 1 2 3 TaleranfR


27





3




17
































2





















7

1
1











































































7
















































I






















































































i














































3














•




































































I

F
F







I
I
I
I
Y

F




























-------
              Tablo 14 cant1
              no
        Qualitative Samples
  June               July
12312
                                    Quantitative Samples
  August
1      23
   June               July
12312
       Augus c
312
                                                                                                                          Tolerance
GASTROPODA (cont'd)
Hydrobla nicklir.lona
Lyanaea sp.
Fhysa elliptic^
Physa Integra
Physldae
Planorbldae
Pleurocera acuta


Promenetus exacuous
Valvata sp.
Valvata crlcarlnaca
Unidcntiflcs sp.
OTHER
BRYOZOA
TURBELLARIA
Cura foremanii
COELESTERATA
Hydra sp.
Hydracarina sp.
KEMATOOA





It














9







30 -














2
1





10


1
169









12

1

1





5







































1









































































































































4






















6















































































































































































































— '


F

T-F


F




I


































-------
Table 14 conc'd
111
Qualitative Sump lea t Quantitative Samp lea
June July August June July August
113123123 123123123 Tolerance
OTHER (cont'd)
NmATOMORPIlA
Paragordlus sp.
TURBELLARIA
Cura foreman!!

Total Taxa


















37

22


















-2 '

25












-







32


















3

16


















3

33


















3

34















t




6




















NS




















f>









































7
















1



19




















12




















2




















2




















10




















2




















3




















3









1























































1































-------