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EPA/600/R-13/109
August 2013
Combustion of Contaminated Animal Carcasses in a Pilot-scale Air Curtain Burner
U.S. Environmental Protection Agency
Office of Research and Development
National Homeland Security Research Center
Decontamination and Consequence Management Division
Research Triangle Park, NC 27711
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EPA/600/R-13/109
August 2013
NOTICE
The U.S. Environmental Protection Agency (EPA), through its Office of Research and
Development's (ORD's) National Homeland Security Research Center (NHSRC), funded and
managed the research described here through Contract Number EP-C-09-027 with ARCADIS
US. It has been subjected to the Agency's administrative review and approved for publication.
The views expressed in this report are those of the authors and do not necessarily reflect the
views or policies of the Agency. Mention of trade names or commercial products does not
constitute endorsement or recommendation for use.
Questions concerning this document or its application should be addressed to:
Paul Lemieux
National Homeland Security Research Center
Office of Research and Development
U.S. Environmental Protection Agency
Mail Code E343-06
Research Triangle Park, NC 27711
919-541-0962
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EXECUTIVE SUMMARY
Tests were run on a pilot-scale Air Curtain Burner (ACB) burning a combination of wood and
poultry carcasses inoculated with Geobacillus stearothermophilus as a surrogate for pathogenic
Foreign Animal Disease (FAD) organisms. The tests were run to evaluate emissions of
potential pathogens (using spores of a nonpathogenic bacterium as a surrogate) as a function of
degrading system combustion efficiency due to overcharging of poultry carcasses. The
objective of the tests was to evaluate whether pathogens might be released from an ACB
system as air emissions or in ash of the ACB as the functions of the ACB began to degrade due
to operational issues, or whether effective pathogen destruction still occurred in spite of
potentially increased air emissions from the ACB due to operational issues. If environmental
performance degrades prior to a reduction in pathogen destruction, operational adjustments and
corrective actions could possibly be made quickly enough, potentially based on easily
observable increases in visible emissions without increasing the risk of pathogen release.
The tests occurred in a pilot-scale ACB located inside the Burn Hut of the EPA's Open Burning
Test Facility located in Research Triangle Park, NC. Bioaerosol samples were taken in the
exhaust duct from the Burn Hut; ash samples were collected from the materials deposited on
the floor of the Burn Hut, together with the bone fragments and ash that remained behind in the
ACB after the tests. Wipe samples were taken after each test to assess whether spores were
deposited on the walls of the Burn Hut. Two test conditions were used, one with a wood:poultry
mass feed ratio of approximately 2:1, representing normal operation and one with a
wood:poultry mass feed ratio of approximately 0.66:1, representing an operational regime with
higher poultry input rates than recommended operating procedures would suggest.
No viable spores (measured as colony forming units, CFU) were found in the bioaerosol
samples taken from the exhaust duct from the Burn Hut. Some CFU were found associated
with the larger ash particles that left the ACB and fell to the floor of the burn chamber in close
proximity to the ACB. No viable spores were found in the residual ash left behind in the ACB
after the tests, although CFU were detected in the bone fragments in one test. No CFU were
found on wipe samples of the walls of the burn chamber after each test.
Within the limitations of the experimental apparatus and associated detection limits, it does not
appear that measurable quantities of viable spores were released into the air from combustion
of the inoculated poultry even at poultry feed rates that resulted in pollutant emissions at least a
factor of five higher than the baseline ACB emissions while burning wood only.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
TABLE OF CONTENTS
EXECUTIVE SUMMARY i
TABLE OF CONTENTS ii
LIST OF TABLES v
LIST OF FIGURES vi
ACRONYMS AND ABBREVIATIONS viii
ACKNOWLEDGMENTS x
1.0 INTRODUCTION 1
1.1 Purpose of the Study 1
1.2 Facility Used for the tests 1
1.3 Project Objectives 2
2.0 EXPERIMENTAL APPROACH 4
2.1 General Approach and Test Conditions 4
2.1.1 Combustible Fuel Component 4
2.1.2 Dead Livestock Component 5
2.1.3 Feed Charge 5
2.1.4 Process 5
2.1.5 Pilot-Scale ACB Description 5
2.1.6 Burn Hut, Baghouse and HEPA Filter 6
2.1.7 Feed Chute 7
2.1.8 Air Curtain Nozzle Assembly, Air Supply, and Controls 8
2.2 Sampling 8
2.2.1 Sampling/Monitoring Points 9
2.2.2 Frequency of Sampling/Monitoring Events 9
2.3 Approach for Evaluating Project Objectives 9
2.3.1 Scoping Tests 9
2.3.2 Phase 1 Range Scoping Tests 10
2.3.3 Phase 1 Targeted Tests 10
2.3.4 Phase 2 Tests 10
3.0 EXPERIMENTAL PROCEDURES 11
3.1 Method to Establish Steady-State Conditions 11
3.2 Site Preparation 11
3.2.1 Burn Hut Preparation and Post-Test Cleanup 11
3.2.2 Measurement Systems Startup 11
3.2.3 ACB Startup 11
3.2.4 Poultry Feeding 12
3.2.5 Post Poultry-Feed Sustained Burn 12
3.2.6 Post-Fuel Feed Burnout 12
3.3 Sample Collection, Transport, and Storage 13
3.3.1 Wood 13
3.3.2 Poultry 13
3.3.3 ACB Residual Ash 13
3.3.4 Burn Hut Wall Wipe Samples 13
4.1 Sampling Methods 16
4.1.1 Continuous Emission Monitors 16
4.1.1.1 Burn Hut Exhaust Gas CO2/O2 (EPA Method 3A) 16
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
4.1.1.2 Burn Hut Exhaust Gas CO (EPA Method 10) 16
4.1.2.3 Burn Hut Exhaust Gas THC (EPA Method 25A) 17
4.1.2 Burn Hut Exhaust Gas Volumetric Flow Rate (EPA Methods 1A and 2C) 17
4.1.3 Burn Hut Exhaust Gas Molecular Weight and Moisture (EPA Methods Saand 4). 17
4.1.4 Burn Hut Exhaust Gas Filterable Particulate Matter (EPA Method 5) 18
4.1.5 Burn Hut Exhaust Gas Viable Spores 18
4.1.6 Burn Hut Exhaust Gas PAHs (EPA Method 0010) 18
4.1.7 Burn Hut Exhaust Gas VOCs (Modified EPA Method 0040) 18
4.1.8 ACB Bed Temperature 18
4.1.9 Wood and Poultry Feed Rate 18
4.2 Analytical Methods 19
4.2.1 Particulate Matter Loading 19
4.2.2 Viable Spores, as CFUs, in Burn Hut Exhaust Gas 19
4.2.3 Polynuclear Aromatic Hydrocarbons (PAHs) By Method 0010 19
4.2.4 VOCs by Method 0040 20
4.2.5 Viable Spores, as CFUs, in Ash 21
4.2.6 Positive and Negative Controls for Spore Samples 22
5.0 RESULTS 24
5.1 Progression of Tests 24
5.2 Continuous Emission Monitoring and Thermocouple Data 29
5.2.1 Real-Time CEM and Thermocouple Traces 29
5.2.1.1 Shakedown Test with Poultry (11-17-2010) 29
5.2.1.2 Test with Detailed Pollutant Measurements under Baseline Conditions 32
5.2.1.3 Tests Varying Amount of Poultry Feed to ACB 34
5.2.1.4 Test with Detailed Pollutant Measurements under Maximum Poultry Feed Rate
Conditions 36
5.2.1.5 Biosampling Tests under Baseline Poultry Feed Conditions 38
5.2.1.6 Biosampling Tests under Maximum Poultry Feed Conditions 46
5.2.2 Average CEM and Thermocouple Data 54
5.3 Extractive Sampling Results 60
5.4 SKC Biosampler Results 62
5.5 Residual Spores in Ash and Bones 64
5.6 Wipe Sample Results 66
5.7 Positive Control Tests 68
5.8 Estimated Emissions in Emission Factor Units 71
6.0 QUALITY CONTROL EVALUATION REPORT 72
6.1 CEM and Thermocouple Data 72
6.2 PM Data 72
6.3 VOCData 72
6.4 PAH Data 72
6.5 SKC Biosampler Data 72
6.6 Blanks, Positive Controls, and Negative Controls 73
6.7 Summary of QC Observations 73
7.0 CONCLUSIONS 74
7.1 Comparison of Pilot-Scale ACB Emissions to Full-Scale ACB Emissions 74
7.2 Effect of Burning Poultry on Emissions 74
7.3 Emission of Viable Spores from Combustion of Contaminated Poultry 74
7.4 Summary 75
7.5 Recommendations for Future Work 75
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
8.0 REFERENCES 76
APPENDIX A: ISOKINETIC SAMPLING WORKSHEETS
APPENDIX B: PM AND ORGANIC LABORATORY RESULTS
APPENDIX C: BIOLABORATORY RESULTS
APPENDIX D: METROLOGY LABORATORY CALIBRATION
REPORTS
IV
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
LIST OF TABLES
Table 1. Types/Numbers of Samples and Frequency of Sampling Events 9
Table 2. Test Matrix for Phase 1 Range Scoping Tests 10
Table 3. Performance Criteria for Critical Measurements 14
Table 4. Sample Storage, Handling and Hold Times 15
Tables. Target PAHs 20
Table 6. Target VOCs 21
Table 7. Test Activities 24
Table 8. Test Variables for Wood and Poultry Feedstocks 56
Table 9. Average Continuous Measurements (Wood Only) 57
Table 10. Average Continuous Measurements (Wood + Poultry) 58
Table 11. Total Filterable PM Results [milligrams/dry standard cubic meters (mg/dscm)] 60
Table 12. Volatile Organic Compound Sampling Results (ug/dscm) 61
Table 13. PAH Sampling Results [parts per billion (ppb)] 62
Table 14. Bioaerosol Sampling Results 63
Table 15. Residual Spores in Fly Ash (Floor Sweepings) 64
Table 16. Residual Spores in Poultry Bones 65
Table 17. Residual Spores in ACB Bottom Ash 66
Table 18. Results from Wipe Samples after Combustion Tests 67
Table 19. Results from Wipe Samples after Burn Hut Decontamination 68
Table 20. Results from Positive Controls - Wipe Samples 69
Table 21. Results from Positive Controls-SKC Biosamplers 70
Table 22. Estimated Emissions of Various Pollutants in Emission Factor Units 71
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
LIST OF FIGURES
Figure 1. Pilot-Scale Air Curtain Burner Test System Flow Schematic 2
Figure 2. ACB Installed inside the Burn Hut 6
Figure 3. Burn Hut, Baghouse, and HEPA Filter 7
Figure 4. Fuel Feed Ram and Chute 8
Figure 5. Curtain Air Supply Blower, Control Valve and Flow Meter 8
Figure6. Temperatures from Trial Poultry Burn (11-17-2010) 29
Figure 7. O2 and CO2from Trial Poultry Burn (11-17-2010) 30
Figure 8. THC and CO from Trial Poultry Burn (11-17-2010) 30
Figure 9. Fuel Feed from Trial Poultry Burn (11-17-2010) 31
Figure 10. Temperatures from Baseline Pollutant Test (1-20-11) 32
Figure 11. O2 and CO2 from Baseline Pollutant Test (1-20-11) 32
Figure 12. THC and CO from Baseline Pollutant Test (1-20-11) 33
Figure 13. Fuel Feed from Baseline Pollutant Test (1-20-11) 33
Figure 14. Temperatures from Test Varying Poultry Feed (2-2-11) 34
Figure 15. O2 and CO2 from Test Varying Poultry Feed (2-2-11) 35
Figure 16. THC and CO from Test Varying Poultry Feed (2-2-11) 35
Figure 17. Fuel Feed from Test Varying Poultry Feed (2-2-11) 36
Figure 18. Temperatures from Test at Maximum Poultry Feed Rate (2-16-11) 37
Figure 19. O2 and CO2 from Test at Maximum Poultry Feed Rate (2-16-11) 37
Figure 20. THC and CO from Test at Maximum Poultry Feed Rate (2-16-11) 38
Figure 21. Fuel Feed from Test at Maximum Poultry Feed Rate (2-16-11) 38
Figure 22. Temperatures from BiosamplerTest 1 (2-23-11) 39
Figure 23. O2 and CO2from BiosamplerTest 1 (2-23-11) 39
Figure 24. THC and CO from Biosampler Test 1 (2-23-11) 40
Figure 25. Fuel Feed from BiosamplerTest 1 (2-23-11) 40
Figure 26. Temperatures from Biosampler Test 2 (2-28-11) 41
Figure 27. O2 and CO2from Biosampler Test 2 (2-28-11) 41
Figure 28. THC and CO from Biosampler Test 2 (2-28-11) 42
Figure 29. Fuel Feed from Biosampler Test 2 (2-28-11) 42
Figure 30. Temperatures from BiosamplerTest 3 (3-2-11) 43
Figure 31. O2 and CO2 from BiosamplerTest 3 (3-2-11) 43
Figure 32. THC and CO from Biosampler Test 3 (3-2-11) 44
Figure 33. Fuel Feed from Biosampler Test 3 (3-2-11) 44
Figure 34. Temperatures from Biosampler Test 4 (3-8-11) 45
Figure 35. O2and CO2 from Biosampler Test 4 (3-8-11) 45
Figure 36. THC and CO from Biosampler Test 4 (3-8-11) 46
Figure 37. Fuel Feed from Biosampler Test 4 (3-8-11) 46
Figure 38. Temperatures from BiosamplerTest 5 (3-10-11) 47
Figure 39. O2 and CO2 from BiosamplerTest 5 (3-10-11) 47
Figure 40. THC and CO from Biosampler Test 5 (3-10-11) 48
Figure 41. Fuel Feed from Biosampler Test 5 (3-10-11) 48
Figure 42. Temperatures from Biosampler Test 6 (3-14-11) 49
Figure 43. O2 and CO2from Biosampler Test 6 (3-14-11) 49
Figure 44. THC and CO from Biosampler Test 6 (3-14-11) 50
Figure 45. Fuel Feed from Biosampler Test 6 (3-14-11) 50
Figure 46. Temperatures from BiosamplerTest 7 (3-16-11) 51
Figure 47. O2 and CO2 from BiosamplerTest 7 (3-16-11) 51
Figure 48. THC and CO from Biosampler Test 7 (3-16-11) 52
Figure 49. Fuel Feed from Biosampler Test 7 (3-16-11) 52
VI
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Figure 50. Temperatures from BiosamplerTest 8 (3-21-11) 53
Figure51. O2 and CO2from BiosamplerTest 8 (3-21-11) 53
Figure 52. THC and CO from BiosamplerTest 8 (3-21-11) 54
Figure 53. Fuel Feed from Biosampler Test 8 (3-21-11) 54
Figure 54. Average Temperatures with the ACB under Different Test Conditions 59
Figure 55. Average CO and THC from Various Test Conditions 59
VII
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
ACRONYMS AND ABBREVIATIONS
ACB
APPCD
ATCC
BD
BH
BL
CEM
CPU
CO
CO2
DAS
Dl
EF
EPA
FAD
FMD
GC
Gs
HASP
THC
HEPA
HHV
HRGC/LRMS
ID
mg/dscm
ml_
L
Ib
MOP
MS
ng
Air Curtain Burner
Air Pollution Prevention and Control Division
American Type Culture Collection
Becton Dickinson
Baghouse
Biocontaminant Laboratory
Continuous Emission Monitor
Colony Forming Unit(s)
Carbon monoxide
Carbon dioxide
Data Acquisition System
Deionized
Emission Factor
U.S. Environmental Protection Agency
Foreign Animal Disease
Foot and Mouth Disease
Gas Chromatograph(y)
Geobacillus stearothermophilus
Health and Safety Plan
Hydrocarbons
High Efficiency Particulate Air
Higher Heating Value
High Resolution Gas Chromatography/Low Resolution Mass
Spectrometry
Induced Draft
Milligram(s) per dry standard cubic meter
Milliliter(s)
Liter(s)
Pounds
Miscellaneous Operating Procedure
Mass Spectrometer(try)
Nanogram(s)
VIM
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
NHSRC
NDIR
NMOC
02
OBTF
ORD
PAH
PBS
PBST
PM
PPE
psi
rpm
QAPP
s
SCFM
TC
THC
ISA
TSB
USDA
VOC
W/rrv°C
w.c.
National Homeland Security Research Center
Non-dispersive Infrared
Non-methane Organic Compound
Oxygen
Open Burn Test Facility
Office of Research and Development
Polycyclic Aromatic Hydrocarbon
Phosphate Buffered Saline
Phosphate Buffered Saline with 0.05% Tween® 20
Particulate Matter
Personal Protective Equipment
Pounds per square inch
Revolutions per minute
Quality Assurance Project Plan
Second(s)
Standard cubic foot/feet per minute
Thermocouple
Total Hydrocarbons
Tryptic Soy Agar
Tryptic Soy Broth
U.S. Department of Agriculture
Volatile Organic Compound
Thermal conductivity in Watts per meter per degree Celsius
Water column
IX
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
ACKNOWLEDGMENTS
The author would like to acknowledge a number of people who have been instrumental in
performing this work. The experiments were performed by ARCADIS U.S., Inc., under Contract
Number EP-C-09-027. The Pilot-Scale ACB was designed and constructed under EPA contract
number EP-C-05-060, Task Order 72. Joe Wood and Worth Calfee of EPA's National
Homeland Security Research Center were extremely helpful during the design of the
experiments and analysis of the biolaboratory data.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1.0 INTRODUCTION
1.1 PURPOSE OF THE STUDY
In the event of a Foreign Animal Disease (FAD) outbreak, the U.S. Environmental Protection
Agency (EPA) is designated, under Homeland Security Presidential Directive 9 [1], to be a
support Agency to support U.S. Department of Agriculture's (USDA's) efforts to respond to an
FAD event. The USDA response will likely include decontamination of agricultural sites,
depopulation of affected (or potentially exposed) livestock, and disposal of the resulting animal
carcasses. For highly contagious FAD agents such as Foot and Mouth Disease (FMD) virus,
on-farm disposal may be preferred so that additional contagion is minimized.
One potential transportable technology for on-farm treatment of contaminated animal carcasses
is Air Curtain Burners (ACBs). ACBs can be mobilized to where they are needed as a potential
means of reducing the waste volume while minimizing potentially harmful environmental
impacts. Operated as an above ground installation, or in some instances, installed over a pit
with the top of the unit at ground level, ACBs are mobile incinerators that utilize the general
concept of a high-volume sheet of air being blown at a slight downward angle across the top of,
and into, the combustion vessel. The air serves a dual purpose: 1) combustion is enhanced
(when compared to open burning) by providing a steady supply of forced excess air with
turbulent mixing resulting in higher temperatures and more thorough consumption of the solid
organic material used as fuel; and 2) the injection of the air at a slightly incident angle forms a
"curtain" that creates a recirculation zone which serves to encourage burnout of unburned
materials. ACBs have been deployed on numerous occasions for the reduction of vegetative
waste and in some cases for destruction of animal carcasses [2]. A detailed analysis of
available literature on ACB emissions can be found in Miller and Lemieux [3]. Typically, when
processing animal carcasses, a mass ratio of fuel to carcasses ranges between 1:1 and 2:1 [2].
An ACB processing 50 tons per day of animal carcasses would therefore require between 50
and 100 tons of wood per day. The large fuel requirement is one of the logistical difficulties in
processing animal carcasses in an ACB.
The purpose of this project was to test, on the pilot scale, operational issues (e.g., air emissions,
pathogen destruction, feed rates) related to processing large amounts of animal carcasses in an
ACB.
This pilot-scale test is not intended to conclude that this technology is suitable for operational
use in an outbreak of a pathogenic FAD. While the results may support its use, an operational
use of this technology for an FAD outbreak may require air permit applications and approval
from appropriate state and local regulatory agencies. The permit application process may result
in monitoring, reporting, operational restrictions, and controls of both air emissions and ash
disposal. In addition, the Clean Air Act also provides for public participation in the permitting
process. These considerations suggest the need for up-front planning for this technology to be
fully utilized in an FAD outbreak.
1.2 FACILITY USED FOR THE TESTS
The facility used for these tests was a pilot-scale ACB system located in the EPA's Air Pollution
Prevention and Control Division's (APPCD's) Open Burn Test Facility (OBTF) [4]. Figure 1
shows a diagram of the OBTF with the ACB installed inside the Burn Hut along with
thermocouple positions.
There are a number of devices on the OBTF that would not normally be found on an ACB,
including a baghouse, High Efficiency Particulate Air (HEPA) filter, and Induced Draft (ID) fan.
These devices exist on the OBTF to prevent the release of potential hazardous air pollutants
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
from experimental operations.
Curtain
Air
'•CM
Burn Hut
Baghouse
HEPA
Filter
ID
Fan
Figure 1. Pilot-Scale Air Curtain Burner Test System Flow Schematic
A series of tests was conducted with the pilot-scale ACB. The tests used wood as the primary
fuel for the ACB. Animal carcasses in the form of single individually packaged Cornish game
hens were periodically fed to the unit. Various samples, both of a continuous and batch nature,
were acquired.
1.3
PROJECT OBJECTIVES
The two primary objectives for this study were:
• Determine the maximum feed rate of animal carcasses into the ACB using a constant feed
rate of wood fuel before emissions of products of incomplete combustion (e.g., carbon
monoxide, volatile organics, semivolatile organics) begin to increase significantly above the
baseline levels found when combusting wood with no animal carcasses; and
• Determine the maximum feed rate of animal carcasses into the ACB using a constant feed
rate of wood fuel before significant quantities of microorganisms contained in the animal
carcasses survive the incineration process.
The critical measurement parameters were:
• Mass of wood fed;
• Mass of poultry fed;
• Test time;
• Amount of inoculum in poultry, in terms of colony forming units (CPU);
• Residual viable spores in poultry carcasses and air emissions after combustion;
• Emission rate for CO2/CO (necessary for estimation of combustion rate and combustion
efficiency, also for comparison with full-scale field test results to verify validity of pilot-scale
simulation);
• Emission rate for filterable particulate matter (PM) (comparison with full-scale test results to
verify validity of pilot-scale simulation);
• Emission rate for polycyclic aromatic hydrocarbons (PAHs) as well as volatile organic
compounds (VOCs) (comparison with full-scale test results to verify validity of pilot-scale
simulation);
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Burn Hut exhaust flow rate and moisture content (necessary for calculation of total mass
emission rates of all measured species); and
ACB bed temperatures measured at six different locations.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
2.0 EXPERIMENTAL APPROACH
2.1 GENERAL APPROACH AND TEST CONDITIONS
The tests were conducted in a pilot-scale ACB burning a fixed quantity of wood and using dead
Cornish game hens (complete with feathers and all internal organs) as a surrogate for animal
carcasses containing residual FAD pathogens. Some tests were conducted with poultry in
uninoculated form and some tests were conducted with inoculated poultry. The inoculum was 1
milliliter (ml_) of Geobacillus stearothermophilus (Gs) spores at a concentration of 1E4 colony
forming units (CFU)/ml_. Gs is a thermally resistant spore, and using Gs for these tests would
thus be a very conservative surrogate for an FAD virus.
Cut lumber was used as the combustible fuel component of the feed material. The testing was
designed to collect and characterize the emissions from burning these materials and to
evaluate/establish ACB operational parameters that would yield acceptably low emissions.
The testing was divided into two Phases:
• Phase 1: operation of the ACB on uninoculated poultry to identify the effect of poultry feed
rate on operational conditions and emissions as measured by continuous measurement
methods and occasional extractive sampling methods (e.g., carbon monoxide, VOCs,
PAHs); and
• Phase 2: operation of the ACB on inoculated poultry under selected operating conditions
identified during Phase 1 to examine survival of residual microorganisms under the
conditions that resulted in elevated emissions.
Replicate test runs were performed to generate and collect sufficient numbers of samples to
produce statistically significant data. Combustion performance was evaluated by continuous
emission monitor (CEM) measurements of the ACB exhaust gas concentrations and by fuel bed
temperatures as measured by thermocouples. Combustion gas- and solid-phase samples
included the feed materials, the combustion residue ash, the particulate matter, VOCs, and
PAHs in the exhaust gases from the ACB, as collected with probes located in the Burn Hut
exhaust duct. Samples for residual spores were collected from the gas phase as well as from
the residual ash in the ACB and the Burn Hut walls and floor. The Burn Hut's baghouse
captured the exhaust particulates, including any residual spores. The baghouse particulate
catch was not used as samples.
Due to the small size of the pilot-scale ACB relative to a full-scale ACB, it was important to use
as homogeneous feed material as possible to assure that the differences between
measurements from these controlled experiments would be due to the test parameters and not
random fluctuations due to feed heterogeneities.
2.1.1 Combustible Fuel Component
Although a full-scale ACB processes raw timber and land clearing debris (e.g., logs, stumps),
due to the small size of the pilot-scale ACB and the need for relatively homogenous solid fuel for
experimental purposes, unprocessed biomass was not used. For these experiments the
combustible fuel was comprised of wood in the form of standard 2-inch x 2-inch pine lumber
purchased at a home improvement store. Previous tests showed that the lumber could be
ignited easily, and its combustion could be sustained without difficulty. The "two x two"
dimensional pieces of lumber have a true cross-sectional dimension of 1.5-inch x 1.5-inch and
they were cut to nominally 12-inch lengths to facilitate feeding into the ACB. A few pieces were
cut shorter than 12 inches to allow the assembly of wood loads that weighed in at the target
charge weight (in the range of 4.25 pounds (Ib) ±0.1 Ib).
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
2.1.2 Dead Livestock Component
The dead livestock component of the feed was Cornish game hens, complete with feathers,
heads, and internal organs (nominal weight per bird = 2-3 Ib). The dead birds were fed into the
ACB at periodic intervals as per the test matrix.
The birds arrived from the supplier dead, frozen, and packaged individually in plastic bags
(polypropylene polymer, Hefty Slide-Rite Bag, Model S-10837). The birds were maintained in a
freezer at 20 °F until required for testing, at which point they were moved to a refrigerator (-40
°F) for 3-4 days to thaw. The thawed birds were weighed and placed into a plastic container
(U.S. Plastics Corporation, Model #85122) as secondary containment. Birds requiring
inoculation were transported to the EPA's Biocontaminant Laboratory (BL), also located in
Research Triangle Park, NC, in the plastic containers and inoculated by injecting 1.0 ml_ of
inoculum (1E6 spores in 10 ml_ Phosphate Buffered Saline (PBS), which translated to 1E4
CFU/mL) of Geobacillus stearothermophilus (American Type Culture Collection (ATCC) #
12980) through the plastic bag into the bird using a syringe and needle (Becton Dickinson (BD)
Precision Guide). The plastic bag containing the inoculated birds was returned to the plastic
container and placed into the refrigerator (40 °F) until needed for testing. The mass of the
plastic bags was small relative to the mass of the birds and should have added only negligibly to
the pollutants.
2.1.3 Feed Charge
Feed charges consisting of the target weight of wood and the poultry were pre-weighed prior to
each of the tests. Based on the assumption that a 4-hour run period would be needed for each
test and that the ACB would be fed at 10-minute intervals, 24 pre-weighed wood charges were
required for each test. Assuming that poultry would be introduced for three hours with each
wood charge, 18 dead birds were nominally required for each run. However, an objective of the
tests was to identify how rapidly the birds could be fed to the ACB without resulting in increased
emissions of products of incomplete combustion. In general, handling of the dead birds by the
test operators was kept to a minimum, and protective gloves were used when handling the
birds.
2.1.4 Process
As presented earlier, Figure 1 shows a schematic diagram of the pilot-scale ACB and Burn Hut
arrangement. Combustion of the feed took place in the ACB inside the Burn Hut with the
combustion air being supplied via a blower into the ACB plenum. Movement of exhaust gases
was driven by an induced draft fan which drew air into the Burn Hut, through the exhaust ducts,
the baghouse and a HEPA filter. These devices are not normally found on full-scale ACBs, but
are components of the OBTF that serve to control operation inside the Burn Hut, to prevent
potential hazardous air pollutants from being emitted, and to provide operator safety. Operation
of the induced draft and the forced draft blowers was adjusted to maintain the Burn Hut static
pressure slightly lower than atmospheric pressure to prevent fugitive leaks of the Burn Hut
combustion gases.
2.1.5 Pilot-Scale ACB Description
The pilot-scale ACB is a rectangular, refractory-lined open-top steel box, with an outside
dimension of 32 inches x 23 inches and 18-5/8 inches tall. Three ports designed for
thermocouple insertion are located on the opposing 32-inch long sides, at 3-5/8, 8-1/8 and 12-5-
/8 inches below the top opening surface. The steel shell is lined with a 4 inch thick layer of
refractory [Harbison Walker KS-4, thermal conductivity 0.9 Watts/meter - degree Celsius (W/m-
°C)] on the four sides and the bottom. The refractory is secured to the steel shell with refractory
anchors (Harbison Walker VS-30-304) welded to the steel shell in a square pattern, 6 inches
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
apart for the vertical walls and 9 inches apart for the bottom wall.
The dimensions of the ACB combustion chamber are 24 inches x 15 inches and 14 -3/8 inches
deep. Photographs of the ACB installed inside the Burn Hut are shown in Figure 2.
These dimensions represent approximately a 1:10 scale model with respect to length and width
and a 1:600 scale model with respect to volume when compared to a full-scale ACB, such as
the Air Burners Model S-327 (firebox dimensions 27 feet 2 inches x 8 feet 5 inches x 8 feet 1
inch) [5].
As an integral part of the ACB combustion chamber, a stainless steel tray was placed inside the
ACB combustion chamber. The purpose of this tray was to facilitate easy ash sample retrieval.
At the conclusion of a test, the entire tray, with its ash content, could be lifted out of the ACB
chamber. A matching steel lid was placed on top of the tray to prevent sample loss during the
time before the ash sample was transferred into the proper sample containers.
2.1.6
Figure 2. ACB Installed inside the Burn Hut
Burn Hut, Baghouse and HEP A Filter
Figure 3 shows the Burn Hut, exhaust duct work, the baghouse, and the High Efficiency
Particulate Air (HEPA) filter. The Burn Hut is 10 ft x 12 ft x 7 ft tall at the side, with a sloped
roof. The highest point of the roof is approximately 8-1/2 ft. During previous testing, the
temperature of the ACB exhaust gas inside the Burn Hut reached a maximum of approximately
230 °F, which is below the maximum service temperature of the Burn Hut, and with the cooling
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
of the exhaust duct, results in baghouse and HEPA filter inlet temperatures below their
maximum temperature ratings. The inside walls of the Burn Hut were lined with heavy-duty
aluminum foil, the function of which is to facilitate easy cleaning and decontamination of the
walls.
The baghouse unit, an integral part of the OBTF, is a Model 72RT-21 manufactured by MAC
Equipment (Sabetha, Kansas, USA).
The HEPA filter (AstrolCel I Model 905-000-348, American Air Filter, Louisville, Kentucky, USA)
has dimensions of 24 inches x 24 inches x 11 1/4 inches. The HEPA filter is rated for a
maximum service temperature of 200 °F and has an efficiency of 99.99% for 0.3 urn particles.
The Burn Hut is operated at a slightly negative pressure (approximately 0.25 inches of water
[w.c.]) so that all gases and aerosols generated inside the Burn Hut leave through the exhaust
duct and pass through the baghouse. This slight negative pressure prevents the escape of
pollutants from inside the Burn Hut without allowing significant quantities of ambient air to
infiltrate into the Burn Hut and dilute the gaseous emissions.
Figure 3. Burn Hut, Baghouse, and HEPA Filter
2.1.7 Feed Chute
The gas temperatures inside the Burn Hut during a test can exceed 200 °F. The gas can also
be laden with fly ash and potentially live spores (even though the spores used in this testing are
nonpathogenic). It is therefore not advisable for the ACB fuel loading operation to require a test
operator to enter the Burn Hut for safety reasons. In addition, opening and closing the Burn Hut
access door will introduce disturbances to the temperature and gas flow conditions inside the
Burn Hut and will create poorly characterized ACB operating conditions.
A round opening was therefore created on one side wall of the Burn Hut to allow a stainless
steel pipe to be installed and function as a feed chute (see Figure 4). With the feed ram
retracted, a simulated feed charge can be placed into the receiver opening of the chute. The
feed ram is then manually pushed in to deliver the charge into the ACB.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Figure 4. Fuel Feed Ram and Chute
2.1.8 Air Curtain Nozzle Assembly, Air Supply, and Controls
The key feature of the ACB is the "air curtain", which is formed by injecting a controlled and
known flow rate of air across the opening of the ACB chamber at an angle that would create the
intended flow field inside the chamber to promote efficient combustion.
The air curtain for the pilot-scale ACB is created by introducing the air via a bank of 107 drilled
round openings, 0.116 inches in diameter, into the side of a steel pipe (schedule 160) with a 3-
inch thick-wall.
The curtain air is supplied by a high pressure blower installed just outside the Burn Hut. The air
flow rate is measured by a rotameter and can be adjusted by a flow control valve (see Figure 5).
2.2
Figure 5. Curtain Air Supply Blower, Control Valve and Flow Meter
SAMPLING
The sampling strategy focused on using continuous measurement methods (fixed combustion
gases, temperatures) to establish sets of operating conditions where more detailed sampling,
using extractive sampling methods, was performed.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
2.2.1 Sampling/Monitoring Points
Six sampling locations were used for these experiments:
• ACB firebox;
• ACB bottom ash residue;
• Burn Hut inside floor (sweepings);
• Burn Hut inside wall (wipe samples);
• Burn Hut exhaust duct gas-phase sample port; and
• Baghouse inlet gas-phase sample port.
2.2.2 Frequency of Sampling/Monitoring Events
Table 1 lists the types of samples that were acquired and frequency of those sampling events.
Note that "continuous" refers to samples being acquired automatically at the scanning frequency
of the data acquisition system, nominally at 10 second intervals. Also note that the bottom ash
sample was generated as a single sample.
Table 1. Types/Numbers of Samples and Frequency of Sampling Events
Type of
Run
Sample
Location
Sample
Duration
Scoping
Blank
Sample
Collection
Phase 1
Range
Sampling
Phase 1
Targeted
Sampling
Phase 2
Sampling
Burn Hut
Exhaust
O2, CO,
CO2, THC
Burn Hut
Exhaust
Duct
Continuous
X
X
X
X
X
ACB Bed
Temp
ACB
Firebox
Continuous
X
X
X
X
X
Burn Hut
Exhaust
Flows
Burn Hut
Exhaust
Duct
Continuous
X
X
X
X
X
PM
Burn Hut
Exhaust
Duct
-60 min.
1
1
Burn Hut
Exhaust
PAH
Burn Hut
Exhaust
Duct
~ 60 min
1
1
Burn Hut
Exhaust
VOC
Burn Hut
Exhaust
Duct
-60 min
1
1
Burn Hut
Exhaust
Spores
Burn Hut
Exhaust
Duct
-60 min
1
8
ACB
Bottom
Ash
Spores
ACB
Bottom
Ash
Residue
N/A
1
8
Burn
Hut
Wall
Wipe
Spores
Burn
Hut
Inside
Wall
N/A
1
8
Burn Hut
Floor
Sweepings
Spores
Burn Hut
Inside
Floor
N/A
1
8
N/A - Not Applicable.
THC - Total Hydrocarbons
PAH - Polynuclear Aromatic Hydrocarbons
x - Samples taken continuously over entire duration of test
2.3
APPROACH FOR EVALUATING PROJECT OBJECTIVES
2.3.1 Scoping Tests
The overall project objectives were evaluated as follows. Phase 1 experiments varied the
number of birds fed into the ACB per unit time. The scoping tests provided an initial
determination of likely poultry feeding scenarios. One bird every 10 minutes along with 25 Ib/hr
of wood was decided upon as the baseline test conditions (this represents a wood:poultry mass
feed ratio of 2:1, which is the highest recommended wood:poultry feed rate for ACB field
operations [2].
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
2.3.2 Phase 1 Range Scoping Tests
Once an appropriate scenario for feeding varying amounts of poultry was identified, a series of
tests was performed to examine continuously measured variables as a function of bird feeding
rate. Table 2 shows the test conditions for the Phase 1 Range Scoping Tests. The test
conditions began with no bird feed, and then feeding of 1 bird every 10 minutes was initiated.
After a minimum of four feed events occurred at a given feed rate, the interval between feed
events was reduced, and the process was repeated. When the feed interval between birds was
reduced below 4 min, visible quenching of the ACB combustion process was observed (i.e., the
flames began to be extinguished), along with significant formation of black smoke.
Table 2. Test Matrix for Phase 1 Range Scoping Tests
Wood Feed
Rate (Ib/hr)
25
25
25
25
25
25
Interval
Between
Poultry Feed
Events (min)
N/A
10
8
6
4
2
N/A - Not Applicable
2.3.3
Phase 1 Targeted Tests
Based on the results from the Phase 1 Range Scoping Tests, a set of operating conditions was
identified when the emissions from the ACB appeared to be detrimentally impacted. The lower
threshold feed interval for poultry was established at 4 min, with the upper threshold feed
interval established at 10 min. Tests were operated with isokinetic extractive sampling
procedures at these two conditions. The isokinetic extractive sampling tests were one hour in
length, the same as the scoping tests.
2.3.4 Phase 2 Tests
A series of tests was then conducted under the same poultry feed rate conditions identified in
Phase 1 but with inoculated poultry. For these tests, no isokinetic extractive chemical pollutant
sampling was performed, but aerosol sampling for gas-phase spores as well as wipe sampling
and floor sweeping sampling was performed.
10
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
3.0 EXPERIMENTAL PROCEDURES
3.1 METHOD TO ESTABLISH STEADY-STATE CONDITIONS
Based on previous testing, the nominal fuel input rates were set with wood at 25 Ib/hr. Each test
ran on wood for approximately 1/4 hour prior to poultry being fed. After the tests, the poultry feed
was stopped, and wood continued to be fed for a period of 1 hour prior to cessation of fuel feed.
The additional 1-hour operation with only wood fed was designed to provide an hour of
additional combustion time with no poultry feed to allow for complete combustion of all poultry
material.
The ACB combustion air flow rate was set at approximately 40 standard cubic feet per minute
(scfm) and was supplied by a pressure blower placed outside the Burn Hut. The temperature of
the air was not controlled and was only a few degrees higher than the ambient temperature as a
result of the blower and the heat absorbed across the piping from the hot gas inside the Burn
Hut (approximately 200 °F).
3.2 SITE PREPARATION
3.2.1 Bum Hut Preparation and Post-Test Cleanup
Prevention of cross contamination of test samples between tests with the spores was
necessary. Minimizing the opportunity for fugitive release of spore particles during and after the
tests was also important. Therefore, prior to testing with spores, the inside of the Burn Hut was
cleaned according to the following procedures. The same procedures were also followed after
all testing was completed.
• Don Personal Protective Equipment (PPE) per the approved Health and Safety Plan
(HASP);
• Remove loose particulate materials from all internal surfaces using a vacuum cleaner
(Omega Vac, Atrix International, Burnsville, Minnesota, USA) designed for removal and
capture of toxic dust). A new vacuum filter and a clean inlet hose were used for each
cleanup event to reduce the opportunity of sample cross-contamination;
• Collect vacuum cleaner catch into a labeled sterile plastic bag for archive and analysis;
• Decontaminate the inside surfaces of the Burn Hut and exhaust duct - The primary risks of
cross contamination arise from residual spores present in the Burn Hut and Burn Hut
Exhaust Duct after one test that may remain at the start of the next test. Because of this
possibility of cross-contamination, the Burn Hut and Burn Hut Exhaust Duct were
decontaminated using Spor-Klenz® (STERIS, Mentor, OH) after each test with inoculated
birds. Spor-Klenz® is a hard surface antimicrobial solution that was used to eliminate all
remaining spores in the Burn Hut and associated equipment;
• Obtain wipe samples from the Burn Hut to be analyzed for CPU by the BL;
• Decontaminate the stainless steel tray of the ACB with Spor-Klenz® prior to use in the ACB;
and
• Reline the walls with new aluminum foil after all testing has been completed.
3.2.2 Measurement Systems Startup
Commence operation of the CEMs and data logging functions with the data acquisition system.
3.2.3 ACB Startup
The ACB operation begins with the lighting of a charge of wood, weighing approximately 4.25 Ib,
11
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
in the ACB. A commercially available charcoal lighter fluid was used as fire starting accelerant
because the lighter fluid does not leave a solid residue. A small amount of lighter fluid,
estimated at no more than 50 ml_, was used. Note: PAH/VOC sampling, if performed that day,
was not initiated until at least 45 minutes after startup to allow for complete combustion of the
lighter fluid.
The ACB air flow was started when the wood flame was sufficiently well established, as evident
by the flame not being extinguished by the curtain air. Wood feeding commenced per the set
schedule. Four feed cycles were performed initially to establish steady combustion conditions
(see Section 5.2.1.1 for the range of temperatures in the ACB at what was considered steady
conditions). The air curtain flow rate was adjusted to correspond to the combustion conditions
and was eventually increased to the set point level of 40-41 scfm.
Steady combustion conditions were considered to have been reached when the bed
temperatures measured over three charge cycles were unchanged, as determined by the
observation of a cyclic temperature trace where local minimum and maximum temperatures in
each cycle were within 50 °F of each other. The 20-minute running averages of the measured
ACB bed temperatures were calculated to serve as indicators to evaluate whether steady-state
combustion conditions had been reached. Moreover, the ACB process is recognized to be
semi-batch and the measured temperatures during the burn cycle for each charge do not follow
exactly duplicable patterns. The determination is therefore recognized to be intrinsically
subjective to a degree.
3.2.4 Poultry Feeding
Upon establishing stable combustion, feeding of wood and poultry loads commenced. Feeding
of wood/poultry loads at set intervals (tentatively 10-min) continued for the duration of the test,
which was approximately 3 hours.
Sampling for PAH with Method 0010 and PM (Method 5) in the Burn Hut exhaust gas lasted
nominally 60 minutes, depending on the particulate loading in the sampled gas. Aerosol
sampling for spores required a minimum of 60 minutes.
In any event, test duration was typically not shorter than three hours due to the need to
generate samples (gas and ash) after reasonably steady-state conditions had been established.
Trial tests showed that up to an hour was required to establish steady ACB operation
conditions.
3.2.5 Post Poultry-Feed Sustained Burn
After the last charge of the wood/poultry load, ACB operation continued for one hour during
which fuel loads consisting of wood only were charged at the set intervals. This operation
period ensured that the last poultry charged was treated at the target temperature for the
specified treatment time. This operation period also reflects typical field operation of ACBs,
where feed of contaminated material is ceased for a period of time prior to cessation of solid fuel
feed. The curtain air flow rate remained at the set-point level.
3.2.6 Post-Fuel Feed Burnout
Upon completion of the Post Poultry-Feed Sustained Burn period, the air curtain air was turned
off completely. The residual combustible materials inside the ACB were allowed to burn out
naturally without the forced convective air currents. The reason for not having the air curtain air
flowing was to minimize the possibility for the exposed fluffy ash material to become airborne
and be blown out of the ACB.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
3.3 SAMPLE COLLECTION, TRANSPORT, AND STORAGE
Table 3 lists the samples, sampling and analytical methods, and performance criteria for critical
measurements. All laboratory analytical instrument calibrations were performed in accordance
with the published methods.
3.3.1 Wood
Randomly selected sections of the 2 x 2 pine lumber were collected and saved as the wood
feed sample.
3.3.2 Poultry
Analysis of the Cornish game hens for inoculum recovery was originally intended. Samples of
the Cornish game hens were ground up in a blender (Waring Laboratories, Torrington, CT, Lab
Blender Model CB15) in the BL. This process proved to be very difficult due to the material
getting hot in the blender due to the energy input from the grinding process. The results for
recovery of inoculum from the meat matrix are therefore not available.
3.3.3 ACB Residual Ash
Each of the combustion tests lasted approximately four hours. As described earlier, the ash
residues that remained in the ACB resided inside the rectangular stainless steel tray. The entire
ash residue remaining in the tray was collected and saved as the ash sample from the test. The
ash was examined and, if reasonably intact pieces of the poultry were present, those pieces
were manually separated from the bulk ash for separate analysis.
3.3.4 Bum Hut Wall Wipe Samples
Wipe samples were collected on the Burn Hut walls and inside the exhaust duct prior to and
after completion of each spore test and after the decontamination of the Burn Hut interior
surfaces was completed. The purpose of these samples was to detect residual spore
contamination in the Burn Hut before and between the spore tests.
All samples were shipped with the appropriate chain of custody information. Analytical
laboratories observed the method-specified hold times. Table 4 lists the specified sample
containers, handling and preservation, laboratories, and the respective sampling run and hold
times for the various samples.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 3. Performance Criteria for Critical Measurements
Measurement
Parameter
Burn Hut Exhaust
Velocity Traverses
Burn Hut Exhaust
Volumetric Flow
Rate
Burn Hut Exhaust
Moisture Content
Burn Hut Exhaust
C02/02
Burn Hut Exhaust
CO
Burn Hut Exhaust
VOCs
Burn Hut Exhaust
PAHs
Burn Hut Exhaust
Total Filterable
Particulate
Burn Hut Exhaust
Spores
Floor Sweeping
Spores
Wall Wipe Spores
Ash Spores
ACB Bed
Temperature
Sampling
Method(s)
EPA Method 1A
EPA Method 2C (to be
performed in conjunction
with M5 and M0010)
EPA Method 4 (to be
performed in conjunction
with M5 and MO0 10)
EPA Method 3A
EPA Method 10
Modified EPA Method 0040
EPA Method 0010
EPA Method 5
Aerosol collection by AGI
Impinger
HEPA vacuum sample
2x2" non-cotton wipe sample
Bulk sample
N/A
Sub-Parameter
N/A
Pilot tube leak check
Gas temperature
Post-test meter calibration
check
Balance calibration check
Calibration error
Sampling system bias
Zero and calibration drift
Calibration error
Sampling system bias
Zero and calibration drift
N/A
N/A
Post-test meter calibration
check
Balance calibration check
N/A
N/A
N/A
N/A
Calibration error
Analysis Method
N/A
Manometer
K-Type
Thermocouple
Standard Meter
Comparison
Gravi metric S-Class
weights
Instrumental
Calibration Gases
Instrumental
Calibration Gases
EPA Method 0040
EPA Method 8270D
Standard Meter
Comparison
Gravi metric S-Class
weights
Dilution -culture
Extraction /dilution-
culture
Extraction /dilution-
culture
Extraction /dilution-
culture
K-type thermocouple
Acceptance
Criteria
(%Bias/Recovery)
N/A
± 1 0% of actual value
±3°F
± 5 % of pre-calibration
±0.5g
±2%
±5%
±3%
±2%
±5%
±3%
N/A
70 to 130% Recovery
± 0.5% of pre-calibration
±0.1g
Data collected only from
plates with 30-300 CFU
±10% (for those plates
counted twice)
Data collected only from
plates with 30-300 CFU
±10% (for those plates
counted twice)
Data collected only from
plates with 30-300 CFU
±10% (for those plates
counted twice)
Data collected only from
plates with 30-300 CFU
±10% (for those plates
counted twice)
±5°C
Completeness
100%
100%
100%
100%
90% of Test Periods
90% of Test Periods
50% (minimum 1 or 2 per test
condition)
Precision criteria ± 20%
50% (minimum 1 or 2 per test
condition)
Precision criteria are ±20%
using duplicates
50% (minimum 1 or 2 per test
condition)
100%
100% for first enumeration,
then 25% of plates with 30 -
300 CFU will be counted by a
second technician
100% for first enumeration,
then 25% of plates with 30 -
300 CFU will be counted by a
second technician
100% for first enumeration,
then 25% of plates with 30 -
300 CFU will be counted by a
second technician
100% for first enumeration,
then 25% of plates with 30 -
300 CFU will be counted by a
second technician
85%
N/A-Not Applicable
14
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 4. Sample Storage, Handling and Hold Times
Sample Type
Wood
Ultimate,
Proximate, HHV
Burn Hut Wall
Aluminum Foil
Liner
Spores (between
runs)
Burn Hut Exhaust
Filterable PM
Burn Hut Exhaust
PAHs
Burn Hut Exhaust
VOCs
Burn Hut Exhaust
Gas Spores
ACB Ash
Spores
Burn Hut Wall Wipe
Spores (after
testing was
complete)
Raw Poultry
Method
Sampling/
Analysis
Grab Sample
Wipe Sample
Method 5
Method 0010
HRGC/LRMS
M0040
Aerosol
collection by
AGI Impinger
H EPA Sock
Vacuum (see
Section 5.3.5
Wipe
Grab Sample
Sampling
Time (min)/
Volume/
Weight
12 in long piece of
2x2 wood
2 sq ft of foil
60 min
180 min
-3000 L
-30 min
Approximately
10-30 min (per
Method 427)
1 50-450 L
15min
1 sq ft of wall
1 Bird
Sample Container
Glass Bottle
50 ml sterile conical
tube
Glass Petri dish
Clear or amber glass
bottle
Clear or amber
glass/
Teflon-lined caps;
XAD cartridges
Tedlar® bags
50 ml conical
Sterile bag
50 ml conical
Plastic Bag
Special Handling/
Preservation
Archive
4°C
None
Samples (excluding
particulate filter)
stored at 4 °C
None
4°C
4°C
4°C
4°C
Analytical
Laboratory
Galbraith
Laboratories
Biocontaminant
Laboratory
Resolution Analytics
Enthalpy Analytical
Enthalpy Analytical
Biocontaminant
Laboratory
Biocontaminant
Laboratory
Biocontaminant
Laboratory
Biocontaminant
Laboratory
Maximum
Hold Time
Unlimited
7 days
None specified
Extracted within 14
days of receipt;
Analyzed within 40
days of extraction
30 days
7 days
7 days
7 days
7 days
HHV-Higher Heating Value
15
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
4.0 TESTING AND MEASUREMENT PROTOCOLS
4.1 SAMPLING METHODS
4.1.1 Continuous Emission Monitors
Continuous instrumental methods employed CEMs to measure concentrations of carbon dioxide
(CO2), oxygen (O2), carbon monoxide (CO), and total hydrocarbons (THC). These instruments
were operated in accordance with EPA Methods 3A [6] (CO2/O2), (CO) [7], and 25A (THC) [8]
as prescribed in 40 CFR Part 60, Appendix A. CEM testing began prior to test material being
fed into the ACB and continued through the burnout period for approximately two hours after the
last material was fed.
Burn Hut exhaust gas samples routed to the CEMs (except the THC monitor) were conditioned
to remove water vapor and particulate matter, which are interfering constituents. The sample
gas going to the THC monitor was heated and maintained at 250-300 °F and filtered with glass
fiber filters. The THC monitor requires the sample to be hot and condensate-free to operate
properly, as some components of THC can be removed by condensation of water.
Components of the sampling system in contact with the sample gas were constructed of Type
316 stainless steel or Teflon to minimize the possibility of surface chemical reactions, which can
affect the accuracy of the measurements. The CO2/O2, THC, and CO sample collection and
conditioning system consisted of a heated probe and a particulate filter, followed by a moisture-
removal trap and an out-of-stack secondary particulate filter. A sample pump (such as Thomas
Model 2107CA 18-TFE, Thomas Division-Sheboygan, Sheboygan, Wisconsin, USA)
transported the effluent sample through a distribution manifold to the analyzers. The
configuration of the sampling system allowed the calibration gases to be injected either directly
into the analyzers or through the complete sample collection and conditioning system.
The concentration signal outputs from the CEMs were connected to a computer-based data
acquisition system (DAS). The DAS used a portable computer and an analog-to-digital
converter. For the purposes of these tests, the data were logged at 6-sec intervals without time
averaging data. The functioning of the DAS was checked by verifying that the indicated signal
levels are in agreement with calibrated instruments such as digital voltmeters, thermocouple
(TC) readouts, etc. These checks were performed by the U.S. EPA Metrology Laboratory
(located in RTP, NC).
All pre-test and post-test calibration procedures were performed as outlined in the specific EPA
methods. The operating principles of the analyzers are described in the following subsections.
4.1.1.1 Burn Hut Exhaust Gas COZ/OZ (EPA Method 3A)
Carbon dioxide and oxygen concentrations were determined by EPA Method 3A - Determination
of Oxygen and Carbon Dioxide Concentrations in Emissions from Stationary Sources
(Instrumental Analyzer Procedure), as described in 40 CFR Part 60, Appendix A [6]. In Method
3A, a continuous gas sample is extracted from the stack and conveyed to instrumental
analyzers for the determination of oxygen and carbon dioxide concentration. Results were used
in the calculation of sampling duct gas molecular weight.
4.1.1.2 Burn Hut Exhaust Gas CO (EPA Method 10)
Carbon monoxide emissions were determined by EPA Method 10 - Determination of Carbon
Monoxide Emissions from Stationary Sources, as described in 40 CFR Part 60, Appendix A [7].
In Method 10, a continuous gas sample is extracted from the sampling duct and conveyed to an
instrumental analyzer (non-dispersive infrared [NDIR] or equivalent) for the determination of
carbon monoxide concentration. Flow data from concurrent EPA Methods 1A and 2C were
16
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
used to calculate carbon monoxide mass emission rates.
4.1.2.3 Burn Hut Exhaust Gas THC (EPA Method 25A)
EPA Method 25A - Determination of Total Gaseous Organic Concentration Using a Flame
lonization Analyzer [8] is applicable over a wide range of total hydrocarbon (THC)
concentrations, from percent levels to low ppm levels. The method does not differentiate the
species that constitute total hydrocarbons, (i.e., methane and nonmethane organic compounds
(NMOCs) are measured together and reported as one concentration as equivalent propane).
Method 25 is specifically designed to measure NMOCs. However, it is not suitable for
measuring concentrations that are less than 50 ppm and was not used for these tests.
In Method 25A, a gas sample is extracted from the source through a heated sample line, if
necessary, and a glass fiber filter; the gas sample is then introduced into a flame ionization
analyzer. Results were reported as volume concentration equivalents (ppm by volume) of the
calibration gas (propane) or as carbon equivalents. The mass emission rate was calculated by
the incorporation of results from EPA Methods 1A and 2C volumetric flow data along with
moisture and molecular weights determined by EPA Methods 3A and 4.
4.1.2 Burn Hut Exhaust Gas Volumetric Flow Rate (EPA Methods 1A and 2C)
Flue gas volumetric flow rates were determined by EPA Method 1A - Sample and Velocity
Traverses for Stationary Sources with Small Stacks or Ducts and EPA Method 2C -
Determination of Stack Gas Velocity and Volumetric Flow Rate in Small Stacks and Ducts
(Standard PitotTube), as described in 40 CFR Part 60, Appendix A [9, 10]. The measurement
location in the effluent stream was selected to minimize angular and cyclonic flow. For these
tests, the measurement location was 5 ft downstream of the Burn Hut exhaust duct inlet to
satisfy minimum downstream diameter requirements necessary for isokinetic sampling
conditions.
Using Method 1A, the duct cross section was divided into an appropriate number of equal areas
and the probe was marked to signify the velocity traverse points. Due to the potential for flow
disturbance in small stacks, the sample extraction and flow measurement were performed apart
from one another. Sampling ports for extractive samples were located eight equivalent
diameters upstream of the velocity sampling ports to allow for the re-establishment of flow
stability. Using Method 2C, a traverse for velocity head and sampling duct gas temperature was
performed using a standard pitot tube and thermocouple probe to minimize flow disturbance.
Sampling duct gas volumetric flow rate was calculated by use of the resultant data, the sampling
duct gas density, and duct cross sectional area. Measurements were performed in conjunction
with each test run for filterable/condensable particulate. Flow data, together with pollutant
concentration data from concurrent methods, was used to calculate pollutant mass emission
rates.
4.1.3 Burn Hut Exhaust Gas Molecular Weight and Moisture (EPA Methods 3a
and 4)
Sampling duct gas molecular weight and diluent concentration was performed by EPA Method
3A - Determination of Oxygen and Carbon Dioxide Concentrations in Emissions from Stationary
Sources (Instrumental Analyzer Procedure) and EPA Method 4 - Determination of Moisture
Content in Stack Gases, as described in 40 CFR Part 60, Appendix A [6, 11]. In Method 3A, a
gas sample is extracted continuously from the sampling duct and conveyed to instrumental
analyzers for the determination of O2 and CO2 concentrations. Diluent gas concentration and
molecular weight were calculated from these results. In Method 4, a gas sample is extracted
from the source with moisture removed and determined gravimetrically and/or volumetrically.
Method 4 samples were taken as a part of the EPA Method 5 and M0010 samples.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
4.1.4 Burn Hut Exhaust Gas Filterable Particulate Matter (EPA Method 5)
Filterable particulate matter sampling was performed according to EPA Method 5 -
Determination of Particulate Emissions from Stationary Sources, as described in 40 CFR Part
60, Appendix A [12]. An exhaust gas sample was withdrawn from the sampling duct
isokinetically through a heated probe and pre-weighed, heated, glass fiber filter into an
impinger/condenser train. The filtered, dried gas was measured with a calibrated dry gas meter,
and the particulate matter captured in the probe and filter was desiccated and weighed.
4.1.5 Burn Hut Exhaust Gas Viable Spores
Bioaerosol sampling during Phase 2 tests was performed using sterile SKC Biosamplers (SKC
Inc., Eighty Four, Pennsylvania, USA) containing 20 ml_ of sterile ViaTrap oil (SKC ViaTrap Oil,
catalog number 225-9599, Eighty Four, PA, USA). The Biosampler was installed downstream
of a water-cooled probe located after the Burn Hut exit duct dilution damper and was operated
at 15 pounds per square inch (psi) (monitored with an in-line pressure gauge), resulting in an
isokinetic flow rate. The Biosampler vacuum was provided by a vacuum pump that maintained
a pressure drop of 15 in. Hg, resulting in a constant 12.5 liters (L)/min flow rate. System
exhaust gas samples were withdrawn from the sampling duct directly into the Biosampler using
an EPA Method 5 sampling train. Due to the water-cooled probe, temperatures of the gases
entering the Biosamplers were well below the temperatures at which spores would be killed due
to thermal effects [13, 14].
4.1.6 Burn Hut Exhaust Gas PAHs (EPA Method 0010)
PAHs from Phase 1 tests were collected by the use of EPA Method 0010 - Modified Method 5
Sampling Train as described in 40 CFR Part 60, Appendix A [15]. A metered flue gas sample
was isokinetically withdrawn from the sampling duct through a heated probe and Teflon-coated
glass fiber filter onto a condenser/XAD-2 packed resin trap for collection of semivolatile
compounds., The filtered dried gas was measured with a calibrated dry gas meter. The XAD
resin trap was extracted, and the extract was split and analyzed for a variety of compounds by
high resolution gas chromatography/ high resolution mass spectrometry (HRGC/LRMS, EPA
Method 8270D [16]). Emission rates were calculated from these results and the results of
concurrent flue gas flow rate measurements using EPA Methods 1A and 2C.
4.1.7 Bum Hut Exhaust Gas VOCs (Modified EPA Method 0040)
VOCs from Phase 1 tests were collected by an adapted version of EPA Method 0040 -
Sampling of Principal Organic Hazardous Constituents from Combustion Sources Using Tedlar®
Bags [17]. In Method 0040, a representative sample was drawn from a source through a heated
sample probe and filter. The sample then passed through a heated three-way valve into a
condenser where the moisture and condensable components were removed from the gas
stream and collected in a glass trap. The gas sample was then collected in a Tedlar® bag.
VOC mass emission rates were calculated from the resultant constituent VOC concentrations
and the results of flue gas volumetric flow rate measurements using concurrent EPA Methods
1A and 2C. Between 20 and 30 L of sample gases was collected.
4.1.8 ACB Bed Temperature
Temperature in the ACB bed area was determined using six K-type thermocouples (Omega,
Stamford, Connecticut, USA), inserted through the refractory lining (see Figure 1). These
thermocouples served to provide wall temperatures during the burn cycle.
4.1.9 Wood and Poultry Feed Rate
As discussed in Section 3.1, batches of the feed materials were weighed prior to the tests. The
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
wood feed materials prepared for a test were weighed ahead of each test, and the weight was
recorded for future use. The birds were weighed and the corresponding feed time was recorded
manually in the test log book. These time and weight records formed the basis of feed rate
determination.
4.2 ANALYTICAL METHODS
4.2.1 Particulate Matter Loading
Filterable particulate matter analyses were performed according to EPA Method 5 -
Determination of Particulate Emissions from Stationary Sources, as described in 40 CFR Part
60, Appendix A [12]. Analysis of all particulate samples was performed by Resolution Analytics
(Resolution Analytics, Inc., of Sanford, NC).
4.2.2 Viable Spores, as CPUs, in Burn Hut Exhaust Gas
Following Phase 2 sample collection, the Biosamplers were allowed to cool, and then aliquots of
ViaTrap oil were ten-fold serially diluted and spread-plated onto tryptic soy agar (TSA). Dilution
and plating procedures are described in BL MOP 6535A [18, 19]. Additional samples were
analyzed qualitatively (growth/no growth) by attempted culture of collected samples. These
procedures were based on BL MOP 6566 [18, 19]. For these qualitative samples, 1 mL of
ViaTrap oil was aseptically transferred from each Biosampler into a sterile vial containing 10 mL
of tryptic soy casein broth (vials prepared according to procedures outlined in BL MOP 6556
[18, 19], except final volume was 10 mL and the sterile tryptic soy broth (TSB) was purchased
rather than prepared in-house) and vortexed for 30 seconds. Negative control samples
consisted of TSB controls and TSB + sterile ViaTrap. Positive controls were comprised of 100
uL of the spore solution used during each test spiked into TSB. All samples and controls were
prepared in triplicate and vortexed for 30 seconds after preparation. The culturing technique
followed that of BL MOP 6566 [18, 19], except that biological indicators were replaced by 1.0
mL of ViaTrap collected from each sampler. Samples were incubated at 55 °C. Plated samples
were incubated for 24 hours and culture tubes were incubated for seven days, as described in
the above-referenced MOPs [18, 19]. CFU were enumerated on TSA plates following
incubation to quantitatively determine recovery based upon equations in BL MOP 6535A [18,
19]. Plates with between 30 and 300 CFU were considered to be within the quantitation range
[20]. Qualitative tubes were assessed after seven days of incubation for growth (turbid growth
media) or no growth (lucid growth media).
4.2.3 Poly nuclear Aromatic Hydrocarbons (PAHs) By Method 0010
Samples collected during Phase 1 tests using Method 0010 were analyzed for PAHs by
Enthalpy Analytical according to EPA/SW846 Method 8270D Semivolatile Organic Compounds
by Gas Chromatography/Mass Spectrometry (GC/MS) [16].
The sample contained a filter, an XAD cartridge, rinses of methylene chloride/methanol and
toluene/acetone (acetone optional) as well as aqueous impinger contents. The methylene
chloride/methanol rinse was extracted with pH 2 water/hydrochloric acid. The water was then
re-extracted twice with methylene chloride. The three methylene chloride extracts were
combined and used in the extraction of the remaining components. The XAD and filter were
Soxhlet-extracted with methylene chloride after spiking with internal standards as required pre-
extraction. The methylene chloride from the rinse was used in this extraction and was poured
through the Soxhlet thimble to catch any particulate that was in the rinse. The methylene
chloride from this extraction was collected and concentrated to final volume. After the
methylene chloride extraction, a second extraction with toluene was done. The toluene rinses
were added to the thimble at this stage. A Dean-Stark apparatus (Prism Research Glass, RTP,
NC, USA) was used to collect the acetone/ methylene chloride and water that were still in the
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
sample. The toluene extract was concentrated to final volume. The impinger solution was
acidified to pH 2 and extracted with methylene chloride. This extract was concentrated to final
volume.
The PAH analysis was performed on a portion of the methylene chloride extract. Table 5 shows
the target PAH compounds. The reporting limits were 4 nanograms (ng)/train.
Table 5. Target PAHs
Naphthalene
Acenaphthene
Phenanthrene
Fluoranthene
Chrysene
Benzo(b)fluoranthene
Benzo(a)pyrene
Dibenzo(a,h)anthracene
Acenaphthylene
Fluorene
Anthracene
Pyrene
Benzo(a)anthracene
Benzo(k)fluoranthene
lndeno(1 ,2,3-c,d)pyrene
Benzo(q,h,i)perylene
4.2.4 VOCs by Method 0040
Analysis of VOCs collected during Phase 1 tests was performed by RTP Laboratories using
EPA Method TO-15, Determination of Volatile Organic Compounds (VOCs) in Air Collected in
Specially-Prepared Canisters and Analyzed by Gas Chromatography/Mass Spectrometry
(GC/MS) as seen in the Compendium of Methods for the Determination of Toxic Organic
Compounds in Ambient Air, Second Edition [21]. Target compounds are shown in Table 6. The
method detection limits for these target compounds as reported by RTP Laboratories are 1
ppmv. The report provided by RTP Laboratories was insufficient due to lack of QC and
calibration data. These analytical data were therefore not used (see Section 6.3). Subsequent
analysis of VOCs was performed by Enthalpy Analytical, Inc., using EPA Method TO-15.
Depending on the concentration of the constituent in the sample, Enthalpy Analytical also used
a method detection limit of 1 ppmv.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 6. Target VOCs
Benzene
Carbon tetrachloride
Chloroform
1,2-Dichlorobenzene
Dichlorodifluoromethane
cis-1 ,2-Dichloroethene
cis-1 ,3-Dichloropropene
Ethylbenzene
Styrene
Toluene
1 ,1 ,2-Trichloroethane
1,1,2-Trichloro-1,2,2-
trifluoroethane
Vinyl chloride
Benzyl chloride
Chlorobenzene
Chloromethane
1,3-Dichlorobenzene
1,1-Dichloroethane
1,1-Dichloroethene
trans-1 ,3-Dichloropropene
Hexachlorobutadiene
1 , 1 ,2,2-Tetrachloroethane
1 ,2,4-Trichlorobenzene
Trichloroethene
1 ,2,4-Trimethylbenzene
m-Xylene & p-Xylene
Bromomethane
Chloroethane
1,2-Dibromoethane (EDB)
1,4-Dichlorobenzene
1,2-Dichloroethane
1,2-Dichloropropane
1 ,2-Dichloro-1 ,1 ,2,2-tetrafluoroethane
Methylene chloride
Tetrachloroethylene
1,1,1 -Trichloroethane
Trichlorofluoromethane
1 ,3,5-Trimethylbenzene
o-Xylene
4.2.5 Viable Spores, as CPUs, in Ash
Bulk ash samples were collected at the end of each experiment in Phase 2. During collection,
ash was segregated into fine ash (expected to be of wood origin) and bulk ash (expected to be
of bird origin). Samples were stored in sterile bags or bottles at 4 °C until analysis. For
analysis, between 0.5 and 25 g of ash was weighed and added to a 50 ml_ sterile vial containing
10 mL of sterile PBS amended with Tween® 20 (BL MOP 6562 [18, 19]). The sample was
sonicated for 45 min to dislodge and suspend any viable spores from the ash. The sample was
then continuously vortexed for two minutes to further dislodge any viable spores. Each vial was
briefly re-vortexed immediately before any solution was withdrawn for analysis. The solution
was subjected to a four-stage serial dilution following BL MOP 6535A [18, 19]. The resulting
samples were plated in triplicate. The subsamples were spread onto solid growth media using
BL MOP 6555 [18, 19]. Plates were incubated at 35 °C ± 2 °C for 18-24 hours. CPU were
counted using BL MOP 6535A [18, 19]. In addition to the analysis in BL MOP 6535A [18, 19],
two additional analysis procedures were used for samples resulting in less than 30 CFU/sample
in the zero tube (undiluted sample, e.g., wipe or vacuum sock in the extraction buffer). First, 1
mL of the extract was filter plated in accordance with BL MOP 6565 [18, 19]. The remainder of
the sample was then filter plated in accordance with BL MOP 6565 [18, 19]. For each partition
(fine ash and bulk ash) the number of viable spores was reported as CFU/g of ash.
Wipe samples were prepared according to BL MOP 6568 [18, 19] and analyzed according to BL
MOP 6567 [18, 19].
Vacuum samples were analyzed according to the following procedural steps:
1. The analyst donned a fresh pair of gloves. Gloves were changed between each of the
samples and after direct contact with a sample to reduce contamination.
2. Sterile 3 oz. specimen cups were pre-labeled per the sample log corresponding to the batch
of samples being processed.
3. The 3 oz. specimen cup sample containers were then loaded with 20 mL of Phosphate
Buffered Saline with 0.05% Tween® 20 (PBST).
4. Both sterile sample bags were opened without removing the inner bag from outer bag. The
HEPA sock assembly was moved to the opening of the bag using the bag.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5. The HEPA sock assembly was removed from the bag by handling only the cardboard
portions.
6. The nonsterile blue portion of the HEPA sock assembly was flipped from the larger
cardboard ring to the smaller ring.
7. The larger cardboard ring was removed, exposing the HEPA sock
8. The HEPA sock was wetted by holding the upper blue portion of the HEPA sock (around the
smaller cardboard ring) and dipping the lower 1inch of the HEPA sock into the PBST. The
HEPA sock was allowed to soak up the PBST for a few seconds.
9. After the soaking, the HEPA sock was lifted up just above the opening of the specimen
bottle. A 1-inch vertical slit was cut up the center from the bottom of the sock using sterile
scissors. A new pair of scissors was used for each sample.
10. The HEPA sock was then cut horizontally from side to side, approximately 1 inch from the
bottom allowing the two pieces to fall into the specimen bottle. The HEPA sock was cut only
where the sock had been wetted (dip, wet, look, cut).
11. Steps 6-8 were repeated until the entire white portion of the HEPA sock had been cut.
12. The upper top blue portion of the HEPA sock was then discarded.
13. After use, scissors were autoclaved using a one-hour gravity destruction cycle in preparation
for use with the next sample batch.
14. Gloves were changed between samples.
15. Steps 4-12 were repeated for each sample in the batch.
16. All samples (up to sixty samples at a time) were loaded into the sample cup holder of the
orbital shaker incubator (Barnstead Lab-line, model number 3626, Melrose Park, IL, USA).
17. The samples were agitated in the shaker incubator at 300 revolutions per minute (rpm) for
30 minutes with the heat off.
18. The samples were then removed from the shaker incubator and taken to the BioSafety
Cabinet (NuAire models NU-430-600 series 29, NU-S121-636-L series SP, and NU-S121-
636-R series SP, Plymouth, MN, USA) for dilution plating as described in MOP 6535A [18,
19]. Plates with between 30 and 300 CPU were considered to be within the quantitation
range [20]. Qualitative tubes were assessed after seven days incubation for growth (turbid
growth media) or no growth (lucid growth media).
4.2.6 Positive and Negative Controls for Spore Samples
Positive controls of spiked ash demonstrated the ability to recover viable spores from that
medium. Spiked Cornish game hens were also analyzed but analytical difficulties made it
impossible to recover spores from the spiked poultry. The process of macerating the poultry
samples resulted in significant heating of the sample (bordering on cooking), so an unbiased
positive control of inoculated poultry was unable to be acquired. This lack of analytical data for
the spiked Cornish game hens would have impacted data quality only if there had been spores
measured in the combustion blank samples or found on the Burn Hut walls prior to feeding
inoculated poultry.
To control for sterility and background (negative controls), unspiked Cornish game hens, wipes,
vacuum socks, ViaTrap oil, and extraction media were analyzed in the same manner as test
samples.
At the end of the main body of the testing, three additional positive control tests were performed:
• Spraying 2E6 CPU of Gs spores directly into the duct: no flame: A test where the spore
inoculum was sprayed into the Burn Hut exit duct, with no combustion occurring in the ACB;
an SKC Biosampler sample and a Via-Cell cassette (Zefon International, Ocala, Florida,
USA) were acquired;
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Spraying 2E6 CPU of Gs spores directly into the Burn Hut: no flame: A test where the spore
inoculum was sprayed into the Burn Hut with no combustion occurring in the ACB; an SKC
Biosampler sample and a Via-Cell cassette were acquired;
Spraying 2E6 CPU of Gs spores directly into the duct with flame: A test where the spore
inoculum was sprayed into the Burn Hut exit duct with wood combustion occurring in the
ACB; an SKC Biosampler sample and a Via-Cell cassette were acquired.
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5.0 RESULTS
This section summarizes the results from the tests. Further details can be found in the
Appendices, including isokinetic sampling sheets (Appendix A), external laboratory reports from
PM, VOC, and PAH sampling (Appendix B), Biolaboratory data reports (Appendix C), and
calibration reports from the U.S. EPA Metrology Laboratory (Appendix D). The plots of the
continuous monitoring data from the gas monitors and thermocouples are included in the body
of the report in their entirety because understanding of the operation of ACBs sometimes
requires an examination of the transient nature of the tests, which is best visualized from the
CEM and thermocouple traces.
The flow rate in the Burn Hut exhaust duct was approximately 1200 scfm, with a linear velocity
of approximately 69 ft/second (s). The exhaust duct temperature varied between 150 and 200
°F for most tests.
5.1
PROGRESSION OF TESTS
Table 7 lists the dates and test activities on those dates as well as the samples that were
acquired on that date. Note that the test date may not necessarily match up with the date code
in the sample ID, because the BL may have initiated their analysis on a subsequent day from
the combustion test.
Table 7. Test Activities
Test Date
Activity
Samples Acquired
Notes
11/17/10
Trial Poultry Burn
CEMs
Thermocouples
Ash
Carcasses
Floor Sweepings
Shaking down CEMs; needed to replace the THC
analyzer and high-range CO analyzer
Operating Biosampler with chilled water, noted oil
becoming more viscous during test, also noted
particulate being collected in oil from sampling,
determined to operate Biosampler with ambient H2O in
condenser
Fed birds in following patterns every 10 min:
Birds on top of wood bundle
Wood bundle, then bird fed
Bird fed, then wood bundle
Wood fed, bird, then wood
11/30/10
Burned wood only to
determine correct
nozzle size for
Biosampler train
Thermocouples
Duct damper fully open, T's in sampling duct ~160°F
after T in ACS had equilibrated
Velocity traverse at sampling point yielded AP=1.2
Value used for determining nozzle size
1/20/11
First Extraction Test; 1
bird/10 minutes with
wood bundle
CEMs
Thermocouples
M5
M8270D
MTO-15/0040
012011 Wipe W1
012011 Wipe W2
012011 Fly Ash 1
012011 Fly Ash 2
012011 ACS Ash
012011 ACBCC
M5 train malfunctioned during first 27 min, but
problems corrected
M5 train was operated additional 20 min after other
trains with birds continuing to be fed
Noted T's inside hut had increased to 240 °F and
T=190 °F at sample point
Fed wood bundles only for additional hour
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Test Date
Activity
Samples Acquired
Notes
2/2/11
Variation Test; feeding
birds at varied time
intervals to determine
max threshold with
wood every 10 min.
CEMs
Thermocouples
Started feeding birds at 10 min intervals then moved to
8 min. Continued to decrease interval to 6 min, then 4
min, then 2 min. Fed birds every 2 min for 12 min.
Noticed decrease in burn rate, changed to every 6 min
Established threshold as one bird/4-6 min
2/9/11
Aborted Run
Problems encountered with T's in hut and duct too
high
Determined that HEPA filter had experienced too high
T in last test and BH bags were coated with soot
Ordered new bags and HEPA filter and replaced
2/16/11
Extraction Test #2;
maximum threshold 1
bird/4 min, wood
bundle/10 min
CEMs
Thermocouples
M5
M8270D
MTO-15/0040.
021711 ACBAsh
021711 ACBCC
021711 Fly Ash 1
021711 Fly Ash 2
021711 Wipe W1
021711 Wipe W3
Noted continued build-up of bird and wood by end of
test
Layer of unburned wood and bird on bottom of ACS
Appears air from plenum cannot reach bottom to fuel
increased combustion rate
2/22/11
Decontamination of
Burn Hut
022211 Wipe W1
022211 Wipe W2
022211 Wipe W3
Decontaminated Burn Hut with Spore-Klenz
W4
(Door)
1
Diagram of Burn Hut Wipe Sample Locations
2/23/11
Bio Sampler Test* 1;
wood and bird/10 min
CEMs
Thermocouples
2-ACB-BS-01 Oil
2-ACB-BS-01
Rinse
022411 ACBAsh
022411 ACBCC
022411 Fly Ash 1
022411 Fly Ash 2
022411 Wipe W1
022411 Wipe W3
022411 Wipe Blank
N/A
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Test Date
Activity
Samples Acquired
Notes
2/24/11
Decontamination of
Burn Hut
022511 Wipe W2
022511 Wipe W4
022511 Wipe Blank
Replaced bags in Baghouse
Replaced aluminum foil on walls
Replaced HEPA filter
2/28/11
Bio Sampler Test #2;
wood and bird/10 min
CEMs
Thermocouples
2-ACB-BS-02 Oil
2-ACB-BS-02
Rinse
030111 ACS Ash
030111 ACBCC
030111 Fly ash 1
030111 Fly ash 2
030111 Wipe W1
030111 Wipe W3
030111 Wipe
Blank.
Problems with HEPA filter; pieces of filter noted in fan
exhaust
3/1/11
Decontamination of
Burn Hut
030211 Wipe W2
030211 Wipe W4
030211 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/2/11
Bio Sampler Test #3;
wood and bird/10 min
CEMs
Thermocouples
2-ACB-BS-03ON
2-ACB-BS-03
Rinse
030311 ACS Ash
030311 ACBCC
030311 Fly ash 1
030311 Fly ash 2
030311 Wipe W1
030311 Wipe W3
030311 Wipe Blank
N/A
3/4/11
Decontamination of
Burn Hut
030411 Wipe W2
030411 Wipe W4
030411 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/8/11
Bio Sampler Test #4;
wood and bird/10 min
CEMs
Thermocouples
2-ACB-BS-04 Oil
2-ACB-BS-04
Rinse
030911 ACS Ash
030911 ACBCC
030911 Fly ash 1
030911 Fly ash 2
030911 Wipe W1
030911 Wipe W3
030911 Wipe Blank
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Test Date
Activity
Samples Acquired
Notes
3/10/11
Decontamination of
Burn Hut
031011 Wipe W2
031011 Wipe W4
031011 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/10/11
Bio Sampler Test #5;
wood/10 min, bird/4
mm
CEMs
Thermocouples
2-ACB-BS-05 Oil
2-ACB-BS-05
Rinse
031111 ACS Ash
031111 ACBCC
031111 Fly ash 1
031111 Fly ash 2
031111 Wipe W1
031111 Wipe W3
031111 Wipe Blank
Noted slight build-up of birds in ACS during test
3/14/11
Decontamination of
Burn Hut
031411 Wipe W2
031411 Wipe W4
031411 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/14/11
Bio Sampler Test #6;
wood/10 min, bird/4
min
CEMs
Thermocouples
2-ACB-BS-06 Oil
2-ACB-BS-06
Rinse
031511 ACS Ash
031511 ACBCC
031511 Fly ash 1
031511 Fly ash 2
031511 Wipe W1
031511 Wipe W3
031511 Wipe Blank
Observed more particulate in bio impinger during test
Grease also in impinger
3/16/11
Decontamination of
Burn Hut
031611 Wipe W2
031611 Wipe W4
031611 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/16/11
Bio Sampler Test #7;
wood/10 min, bird/4
mm
CEMs
Thermocouples
2-ACB-BS-07 Oil
2-ACB-BS-07
Rinse
031711 ACS ash
031711 ACBCC
031711 Fly ash 1
031711 Fly ash 2
031711 Wipe W1
031711 Wipe W3
031711 Wipe Blank
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Test Date
Activity
Samples Acquired
Notes
3/18/11
Decontamination of
Burn Hut
031811 Wipe W2
031811 Wipe W4
031811 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
3/21/11
Bio Sampler Test #8;
wood/10 min, bird/4
mm
CEMs
Thermocouples
2-ACB-BS-08 Oil
2-ACB-BS-08
Rinse
032211 ACS Ash
032211 ACBCC
032211 Fly ash 1
032211 Fly ash 2
032211 Wipe W1
032211 Wipe W3
032211 Wipe Blank
3/23/11
Decontamination of
Burn Hut
032311 Wipe W2
032311 Wipe W4
032311 Wipe Blank
Decontaminated Burn Hut with Spore-Klenz
11/21/11
Bio Positive Control
Test # 1
BS-1 Oil
BS-1 Rinsate
112111 Wipe W1
112111 Wipe W2
112111 Wipe W3
112111 Wipe W4
112111 Wipe Blank
No fire in ACS
Inject 2E6 CFU of Gs spores into hut with nebulizer at
inlet duct (to hut)
All fans operating
Collected sample with ViaCell® cassette in outlet duct
and SKC Biosampler (normal location)
Cassette sampled at 15 L/min vacuum
Noted depletion of liquid in nebulizer by end of test
11/22/11
Bio Positive Control
Test # 2
BS-2 Oil
BS-2 Rinsate
112211 Wipe W1
112211 Wipe W2
112211 Wipe W3
112211 Wipe W4
112211 Wipe Blank
No fire in ACS
Inject 2E6 CFU of Gs spores with nebulizer placed
within 2 feet of entrance to outlet duct
All fans operating
Collected sample with ViaCell® cassette in outlet duct
and SKC Biosampler (normal location)
11/29/11
Bio Positive Control
Test # 3
BS-3 Oil
BS-3 Rinsate
112911 Wipe W1
112911 Wipe W2
112911 Wipe W3
112911 Wipe W4
112911 Wipe Blank
Fire in ACS and with birds not inoculated. Wood and
bird/10 min
Inject 2E6 CFU of Gs spores with nebulizer placed
within 2 feet of entrance to outlet duct
Nebulizer air-line popped off during test; stopped test
momentarily and replaced air-line; decreased pressure
on nebulizer to 30 psi vs. 50 psi
Collected sample with ViaCell® cassette in outlet duct
and SKC Biosampler (normal location)
ViaCell® cassette inlet was wrapped with ice pack to
cool gas before entering cassette
N/A- Not Applicable
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5.2
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
CONTINUOUS EMISSION MONITORING AND THERMOCOUPLE DATA
5.2.1 Real-Time OEM and Thermocouple Traces
5.2.1.1 Shakedown Testwith Poultry (11-17-2010)
Figures 6 through 9 show the CEM results from the shakedown test feeding both wood and
poultry at a rate of one bird every ten minutes. Temperatures stabilized to a cyclic pattern that
coincided with the feeding of the wood and birds. Maximum temperatures in the ACB were
approximately 1000 °C, with approximately a 400 °C range between maximum and minimum
temperatures when the measurements over the entire interior space of the ACB were examined.
This temperature range is not unexpected, given that ambient air is being forced into the ACB at
a downward angle - the temperatures on the side opposite the air input plenum are typically
lower than those on the plenum side. CO and THC increased significantly upon initiation of the
poultry feed and slowly returned to their previous levels during wood-only combustion once
poultry feed ceased. Also note that the CO analyzer reached its maximum concentration at
several points within the run, resulting in a flat trace at that maximum value. A second CO
analyzer that had a higher range was added to the CEM system after this run. The results from
the higher range CO analyzer were used for all subsequent reporting.
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
1200
9:12
10:24 11:36
Time
12:48
14:00
Figure 6. Temperatures from Trial Poultry Burn (11-17-2010)
29
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
—02 —C02
25.0
20.0
15.0
o
"^
ro
01
= 10.0
o
u
5.0
8:00
9:12
10:24 11:36
Time
12:48
14:00
Figure 7. O2 and CO2 from Trial Poultry Burn (11 -17-2010)
350
8:00
—HC —CO
9:12
10:24 11:36
Time
12:48
14:00
Figure 8. THC and CO from Trial Poultry Burn (11-17-2010)
30
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5.0
4.5
4.C
3.5
OJ
52.0
1.5
0.5
0.0
Birds •Wood
8:00
9:12
10:24 11:36
Time
12:48
14:00
Figure 9. Fuel Feed from Trial Poultry Burn (11-17-2010)
31
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5.2.1.2 Test with Detailed Pollutant Measurements under Baseline Conditions
Figures 10 through 13 show the CEM, temperature, and feed rate traces during the test where
wood and poultry were burned under the baseline conditions (25 Ib/hr of wood and 10 min feed
intervals for the birds). Note the cyclic nature of the traces (particularly CO) corresponding to
feed events.
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
Top (Non-Plenum Side)
-Middle (Plenum Side)
Outlet Duct
Baghouse Inlet
—Bottom (Plenum Side)
Top (Plenum Side)
—Burn Hut
1200
8:00
9:12
10:24 11:36
Time
12:48
14:00
Figure 10. Temperatures from Baseline Pollutant Test (1-20-11)
—02 — C02
TC
7O
?
' 1 C
cent ration
-> h
D L
E -L
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
— HC —CO
700
9:12
10:24 11:36
Time
12:48
14:00
Figure 12. THC and CO from Baseline Pollutant Test (1-20-11)
4
3
3"
Iz
1
1
0
» Birds •Wood
c
1
A
D
C
9
c
1 .
••••••••••••H«HnHHH"H'HBHHHlH
• ^ • •
** * ^* ^
8:00 9:12 10:24 11:36 12:48 14:00
Time
Figure 13. Fuel Feed from Baseline Pollutant Test (1-20-11)
33
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5.2.1.3 Tests Varying Amount of Poultry Feed to ACB
Figures 14 through 17 show the CEM, temperature, and feed rate results during the tests where
the feed interval for the poultry carcasses was reduced from 10 minutes to 8, 6, 4, and 2
minutes. Note from the temperature traces in Figure 14 the destabilization of operation that
occurs after approximately 12:00, which was the time when the poultry feed rate was at a
maximum (one bird every two minutes). At that point, several thermocouples exhibited rapid
drops in reported temperatures, and other thermocouples showed cyclic fluctuations much
greater than they showed at lower poultry feed rates. Also note the significant increase in CO
and THC that occurs at this same time. These conditions represent ACB operating conditions
when it would be expected that the operator would observe obvious visual clues that the
combustion effectiveness was deteriorating due to overfeeding of bird carcasses, and
quenching may even be occurring. Therefore it appears that for this particular pilot-scale ACB,
feeding these materials at a feed interval of four minutes per bird appears to be the highest
poultry feed rate at which stable operation can still be maintained (i.e., ACB operation was not
quenched). The baseline case represented a wood:poultry mass feed ratio of approximately
2:1; the high poultry feed rate case represented a wood:poultry mass feed ratio of approximately
0.66:1, which was considerably outside the range of recommended mass feed ratio of fuel to
poultry for normal ACB operation.
A four min poultry feed interval was therefore selected for the second test condition.
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
Top (Non-Plenum Side)
-Middle (Plenum Side)
Outlet Duct
Baghouse Inlet
—Bottom (Plenum Side)
Top (Plenum Side)
—Burn Hut
1200
1000
«_. 800
600
400
200
9:00
10:12
11:24 12:36
Time
13:48
15:00
Figure 14. Temperatures from Test Varying Poultry Feed (2-2-11)
34
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
—02 —C02
25
20
= 15
o
2
4-»
£
OJ
10
o
u
9:00
10:12
11:24 12:36
Time
13:48
15:00
Figure 15.02 and CO2 from Test Varying Poultry Feed (2-2-11)
— HC —CO
1200
9:00
10:12 11:24 12:36
Time
13:48
15:00
Figure 16. THC and CO from Test Varying Poultry Feed (2-2-11)
35
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Birds •Wood
5.00
4.50
4.00
3.50
•3.00
2.00
1.50
1.00
0.50
0.00
9:00
10:12
11:24
12:36
13:48
15:00
Time
Figure 17. Fuel Feed from Test Varying Poultry Feed (2-2-11)
5.2.1.4 Test with Detailed Pollutant Measurements under Maximum Poultry Feed Rate
Conditions
Figures 18 through 21 show the CEM, temperature, and feed rate traces for the tests where
particulate matter (PM), VOCs, and PAHs were measured under the maximum poultry feed rate
conditions. Note that even with backing off the feed rate from two minutes per bird to four
minutes per bird, there was a slight amount of instability in the ACB operation as the amount of
partially and mostly combusted birds in the ACB began to fill the combustion chamber, affecting
the fluid mechanics of the combustion air delivery. This effect may be more significant for the
small-scale ACB tested than larger industrial-scale ACBs used in field, although the poultry to
fuel mass ratio tested is within the bounds of those found in actual practice. Both the effects of
increasing poultry feed rate on fluid mechanics of combustion air supply and on amount of heat
available for supporting poultry combustion may affect scaling up the pilot-scale results for
practical applications in the field.
36
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
9:00
13:48
Figure 18. Temperatures from Test at Maximum Poultry Feed Rate (2-16-11)
—02 — C02
TC n
*?n n
?
*""* 1 c n
0
1
E
OJ
u 1 n n
3
5n
n n
r-^™^-^.^^^^
u
9:00 10:12 11:24 12:36 13:48
Time
Figure 19.02 and CO2 from Test at Maximum Poultry Feed Rate (2-16-11)
37
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1000
900
9:00
— HC —CO
13:48
Figure 20. THC and CO from Test at Maximum Poultry Feed Rate (2-16-11)
5n
4.5
A n
3C
£ 3-0 -
£ 2S -
M"
1
s 9 n
1 c
1 n
Oc
n n
9:
» Birds •Wood
*
V* + < >
•
DO 10:12 11:24 12:36 13:48
Time
5.2.1.5
Figure 21. Fuel Feed from Test at Maximum Poultry Feed Rate (2-16-11)
Biosampling Tests under Baseline Poultry Feed Conditions
Figures 22 through 37 show the CEM, temperature, and feed rate traces for the tests under the
baseline poultry feed conditions (25 Ib/hr of wood and one bird every ten minutes).
38
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
600
400
200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
9:00 9:28 9:57 10:26 10:55 11:24 11:52
Figure 22. Temperatures from Biosampler Test 1 (2-23-11)
—02 — C02
~)C
*?n
?
' 1 C
centration
-> h
D L
E •"•"
O
U
c
f____^^_x_^____v__^ ^_
W »-^ .' -^r -"1 • i ,r- ' r^ TJ
9:00 9:28 9:57 10:26 10:55 11:24 11:52
Time
Figure 23.02 and CO2 from Biosampler Test 1 (2-23-11)
39
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
600
500
—HC —CO
9:00 9:28 9:57 10:26 10:55 11:24 11:52
Figure 24. THC and CO from Biosampler Test 1 (2-23-11)
5nn
4.50
4nn
D cr\
•— - 3 on
_Q J.UU
4_i
.c 7 "^n
M Z'->U
1
s 9 nn
1 en
1 no
Ocn
ODD
9:
* Birds •Wood
... t* **
^
*
*
•
DO 9:28 9:57 10:26 10:55 11:24 11:52
Time
Figure 25. Fuel Feed from Biosampler Test 1 (2-23-11)
40
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
1200
1000
«_. 800
600
400
200
10:59
15:47
Figure 26. Temperatures from Biosampler Test 2 (2-28-11)
—02 —C02
25.0
20.0
15.0
10.0
o
u
5.0
0.0 -
10:59
12:11
13:23
Time
14:35
15:47
Figure 27.02 and CO2 from Biosampler Test 2 (2-28-11)
41
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
—HC —CO
400
350
300
250
X 200
150
100
0
10:59
15:47
Figure 28. THC and CO from Biosampler Test 2 (2-28-11)
5nn
4.50
A no
D cr\
•— - 3 on
_Q J.UU
4_i
_c 7 "^n
M Z'-DU
s 9 nn
1 en
1 no
Ocn
Onn
10
» Birds •Wood
+ + *
* *%»
•
59 12:11 13:23 14:35 15:47
Time
Figure 29. Fuel Feed from Biosampler Test 2 (2-28-11)
42
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
^j I ^*
7:00 7:28 7:57 8:26 8:55 9:24 9:52 10:21 10:50 11:19
Time
Figure 31.02 and CO2from Biosampler Test 3 (3-2-11)
43
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
—HC —CO
900
800
7:00 7:28 7:57 8:26 8:55 9:24 9:52 10:21 10:50 11:19
Figure 32. THC and CO from Biosampler Test 3 (3-2-11)
c nn
4.50
4nn
D Cfl
— . 3 nn
•£ 3.UU
4_i
_G *? "^n
M 2-bU
5 7 on
1 "^n
1 nn
Ocn
Onn
7:
» Birds BWood
4
»* ».»
30 7:28 7:57 8:26 8:55 9:24 9:52 10:21 10:50 11:19
Time
Figure 33. Fuel Feed from Biosampler Test 3 (3-2-11)
44
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
h
D L
E J."
3
r— v-- -^~^—^~^-^—^_^
-1 J
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
Figure 35.02and CO2 from Biosampler Test 4 (3-8-11)
45
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
— HC —CO Hi
700
600
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Figure 36. THC and CO from Biosampler Test 4 (3-8-11)
c nn
4.50
4nn
D Cfl
— . 3 nn
•£ 3.UU
4_i
_G *? "^n
M 2-bU
5 7 on
1 "^n
1 nn
Ocn
Onn
8:
» Birds BWood
* *•*
^^
^
30 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
5.2.1.6
Figure 37. Fuel Feed from Biosampler Test 4 (3-8-11)
Biosampling Tests under Maximum Poultry Feed Conditions
Figures 38 through 53 show the CEM, temperature, and feed rate traces for the tests under the
maximum poultry feed conditions (25 Ib/hr of wood and one bird every four minutes).
46
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
600
400
200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Figure 38. Temperatures from Biosampler Test 5 (3-10-11)
25.0
20.0
1T15.0
o
£
E
OJ
= 10.0 -
o
u
5.0
0.0
8:
—02 — C02
^~-^~- ~~^—^ -^-^ r^^_^
^^^^^.^^^^^^ _____
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
Figure 39.02 and CO2from Biosampler Test 5 (3-10-11)
47
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
900
800
— HC —CO Hi
:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Figure 40. THC and CO from Biosampler Test 5 (3-10-11)
R nn
4.50
A nn
Sen
•— - 3 nn
_Q D.UU
4_i
_c 7 "^n
M Z'-DU
1 "^n
1 nn
Ocn
n nn
8:
» Birds •Wood
.
*
*
* / 4* .
V^w^
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
Figure 41. Fuel Feed from Biosampler Test 5 (3-10-11)
48
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
600
400
200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 42. Temperatures from Biosampler Test 6 (3-14-11)
25.0
20.0
"E" 15.0 -
o
£
E
OJ
= 10.0 -
o
u
5.0
0.0
8:
—02 — C02
^~^— — -v— — —v^- ^^—^ '
__^_
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 43.02 and CO2from Biosampler Test 6 (3-14-11)
49
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1000
900
800
-=• 700
a 600
X 500
400
300
200
100
— HC —CO
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 44. THC and CO from Biosampler Test 6 (3-14-11)
5nn
4.50
4nn
D cr\
•— - 3 on
_Q J.UU
4_i
.c 7 "^n
M Z'->U
1
s 9 nn
1 en
1 no
Ocn
ODD
8:
* Birds •Wood
/o^V^
^
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 45. Fuel Feed from Biosampler Test 6 (3-14-11)
50
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
600
400
200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Figure 46. Temperatures from Biosampler Test 7 (3-16-11)
25.0
20.0
1T15.0
o
£
E
OJ
= 10.0 -
o
u
5.0
0.0
8:
—02 — C02
^-^-^-——^ ^^
/ ~*». .. ~^~ ^
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
Figure 47.02 and CO2from Biosampler Test 7 (3-16-11)
51
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
900
800
— HC —CO
:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Figure 48. THC and CO from Biosampler Test 7 (3-16-11)
5nn
4.50
4nn
D cr\
•— - 3 on
_Q J.UU
4_i
.C 7 "^O
M Z'->U
1
s 9 nn
1 en
1 no
Ocr\
Onn
8:
* Birds •Wood
*+ V4 ^
k + ^* A.
^\ *^ "
*
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50
Time
Figure 49. Fuel Feed from Biosampler Test 7 (3-16-11)
52
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
1000
«_. 800
600
400
200
—Bottom (Non-Plenum Side)—Middle (Non-Plenum Side)
—Top (Non-Plenum Side) —Bottom (Plenum Side)
—Middle (Plenum Side) Top (Plenum Side)
—Outlet Duct —Burn Hut
Baghouse Inlet
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 50. Temperatures from Biosampler Test 8 (3-21-11)
25.0
20.0
1T15.0
o
£
E
OJ
= 10.0 -
o
u
5.0
0.0
8:
—02 — C02
^— ~-~^-^^ _j
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 51.02 and CO2from Biosampler Test 8 (3-21-11)
53
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
1200
— HC —CO Hi
0
8:00 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 52. THC and CO from Biosampler Test 8 (3-21-11)
5nn
4.50
4nn
D cr\
•— - 3 on
_Q J.UU
4_i
.c 7 "^n
M Z'->U
1
s *? nn
1 en
1 no
Ocn
Onn
8:
* Birds •Wood
•
* ^ 4,
* * +*«>
DO 8:28 8:57 9:26 9:55 10:24 10:52 11:21 11:50 12:19
Time
Figure 53. Fuel Feed from Biosampler Test 8 (3-21-11)
5.2.2 Average OEM and Thermocouple Data
Table 8 lists the test variables including the total mass of wood and birds fed into the ACB, as
well as the times for initiation and cessation of wood and poultry feed to the ACB. Table 8 also
lists the calculated average feed rates for wood and poultry over the duration of the test. Table
9 lists the average temperature and CEM measurements for the operation of the ACB on wood
only. Note that for the tests where the poultry feed rate was varied, the average results for
54
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
wood only were calculated based on the timeframe prior to initiation of any poultry feed at all, so
the same results were reported for each poultry feed rate case. Table 10 lists the average
temperature and CEM measurements for the operation of the ACB on both wood and poultry.
In general, the temperatures are relatively consistent between the conditions with and without
poultry. CO and THC are significantly higher (approximately by a factor of three) for the
conditions with poultry feed.
Figure 54 shows the average temperatures at different points in the ACB system for the three
main test conditions (wood only, wood + 1 bird/10 min, wood + 1 bird/4 minutes). The ACB
temperatures are slightly lower for the conditions with 1 bird/4 minutes. Figure 55 shows the
average CO and THC for the various test conditions. It is readily apparent that increasing the
feed rate of poultry while holding the feed rate of wood constant results in an increase in CO
and THC emissions.
55
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 8. Test Variables for Wood and Poultry Feedstocks
Date
11/17/2010
1/20/2011
2/2/2011
2/2/2011
2/2/2011
2/2/2011
2/2/2011
2/16/2011
2/23/2011
2/28/2011
3/2/2011
3/8/2011
3/10/2011
3/14/2011
3/16/2011
3/21/2011
Run Description
Trial Test
Baseline Organic Sampling
Variable Bird Feed 1 Bird/10 min
Variable Bird Feed 1 Bird/8 min
Variable Bird Feed 1 Bird/6 min
Variable Bird Feed 1 Bird/4 min
Variable Bird Feed 1 Bird/2 min
High Poultry Feed Organic Test
BiosamplerTest 1
Biosampler Test 2
BiosamplerTest 3
Biosampler Test 4
BiosamplerTest 5
Biosampler Test 6
BiosamplerTest 7
BiosamplerTest 8
Total
Mass
of
Wood
Fed
(Ib)
131.46
132.58
119.69
119.69
119.69
119.69
119.69
81.45
97.76
80.72
93.48
93.59
85.05
84.67
85.14
85.18
Total
Mass
of
Birds
Fed
(Ib)
36.12
26.57
8.92
7.25
8.74
9.85
16.94
51.12
26.35
20.48
22.12
21.10
47.64
45.22
47.05
48.77
Time
at
First
Wood
Feed
Event
8:10
8:00
9:10
9:10
9:10
9:10
9:10
9:10
9:00
11:10
7:30
8:00
8:00
8:00
8:10
8:30
Time
at
Last
Wood
Feed
Event
13:10
13:00
13:40
13:40
13:40
13:40
13:40
12:10
12:40
14:10
11:00
11:30
11:10
11:10
11:20
11:39
Time
at
First
Bird
Feed
Event
9:30
10:20
10:20
10:58
11:22
11:44
12:00
10:00
10:00
12:30
8:30
9:10
9:00
9:00
9:10
9:30
Time
at
Last
Bird
Feed
Event
12:00
12:00
10:50
11:14
11:40
11:56
12:12
11:16
11:40
13:50
9:50
10:30
10:06
10:12
10:22
10:42
Wood
Feeding
Time
(min)
300
300
270
270
270
270
270
180
220
180
210
210
190
190
190
190
Bird
Feeding
Time
(min)
150
100
30
16
18
12
12
76
100
80
80
80
66
72
72
72
Wood
Feed
Rate
(Ib/hr)
25.4
25.7
25.6
25.6
25.6
25.6
25.6
25.7
25.5
25.5
25.5
25.5
25.5
25.4
25.5
25.6
Bird
Feed
Rate
(Ib/hr)
13.5
14.5
13.4
18.1
21.9
36.9
72.6
38.3
14.4
13.7
14.7
14.1
40.8
35.7
37.1
38.5
56
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 9. Average Continuous Measurements (Wood Only)
Date
11/17/2010
1/20/2011
2/2/201 1
2/2/201 1
2/2/201 1
2/2/201 1
2/2/201 1
2/16/2011
2/23/201 1
2/28/201 1
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
Run
Trial Test
Baseline Organic Sampling
Variable Bird Feed 1 Bird/10
min
Variable Bird Feed 1 Bird/8
min
Variable Bird Feed 1 Bird/6
min
Variable Bird Feed 1 Bird/4
min
Variable Bird Feed 1 Bird/2
min
High Poultry Feed Organic
Sampling
Biosampler Test 1
Biosampler Test 2
Biosampler Test 3
Biosampler Test 4
Biosampler Test 5
Biosampler Test 6
Biosampler Test 7
Biosampler Test 8
Bottom
(Non-
Plenum
Side)
Temp
(°C)
846
821
821
821
821
821
781
699
629
567
733
631
617
667
609
Middle
(Non-
Plenum
Side)
Temp
(°C)
632
630
629
629
629
629
629
672
593
633
623
591
637
612
706
618
Top
(Non-
Plenum
Side)
Temp
(°C)
556
562
549
549
549
549
549
561
508
543
519
486
541
531
536
517
Bottom
(Plenum
Side)
Temp
PC)
725
790
625
625
625
625
625
611
634
701
641
728
563
540
609
592
Middle
(Plenum
Side)
Temp
PC)
619
675
721
721
721
721
721
666
599
613
570
618
602
575
591
560
Top
(Plenum
Side)
Temp
PC)
650
697
680
680
680
680
680
686
679
672
614
606
621
606
615
583
Outlet
Duct
Temp
PC)
73
60
71
71
71
71
71
68
61
89
65
66
66
60
69
71
Burn
Hut
Temp
PC)
87
69
86
86
86
86
86
91
56
103
86
81
78
72
83
80
Bag-
house
Inlet
Temp
PC)
52
63
63
63
63
63
54
79
57
58
58
52
61
63
02
(%)
21
21
21
21
21
21
21
21
21
20
21
21
21
21
21
21
CO2
(%)
0.74
0.20
0.75
0.75
0.75
0.75
0.75
0.80
0.62
0.86
0.70
0.61
0.63
0.64
0.68
0.62
CO
(ppm)
59
65
59
59
59
59
59
34
44
88
63
28
48
64
53
44
THC
(ppm)
5
16
11
11
11
11
11
12
11
19
12
7
11
16
7
10
Note: Averages for wood feeding started after two wood feed intervals and continued until the first bird feed event
Note: Averages for birds started at first bird feed event and continued until one bird feed interval after the last bird feed event
57
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 10. Average Continuous Measurements (Wood + Poultry)
Date
11/17/2010
1/20/2011
2/2/201 1
2/2/201 1
2/2/201 1
2/2/201 1
2/2/201 1
2/16/2011
2/23/201 1
2/28/201 1
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
Run
Trial Test
Baseline Organic Sampling
Variable Bird Feed 1 Bird/10
min
Variable Bird Feed 1 Bird/8
min
Variable Bird Feed 1 Bird/6
min
Variable Bird Feed 1 Bird/4
min
Variable Bird Feed 1 Bird/2
min
High Poultry Feed Organic
Sampling
Biosampler Test 1
Biosampler Test 2
Biosampler Test 3
Biosampler Test 4
Biosampler Test 5
Biosampler Test 6
Biosampler Test 7
Biosampler Test 8
Bottom
(Non-
Plenum
Side)
Temp
(°C)
811
849
872
826
797
817
823
807
747
793
692
536
685
748
794
Middle
(Non-
Plenum
Side)
Temp
(°C)
722
687
647
673
663
720
657
786
752
760
731
632
500
654
807
804
Top
(Non-
Plenum
Side)
Temp
(°C)
648
638
585
621
609
564
558
597
568
589
581
559
602
532
535
652
Bottom
(Plenum
Side)
Temp
PC)
662
786
693
611
570
555
584
521
690
715
670
700
568
508
540
562
Middle
(Plenum
Side)
Temp
PC)
636
683
724
601
671
563
552
583
661
697
669
689
662
554
566
589
Top
(Plenum
Side)
Temp
PC)
731
759
735
680
724
658
613
616
712
673
686
688
497
587
527
568
Outlet
Duct
Temp
PC)
105
83
85
89
91
94
105
74
82
91
88
85
75
71
75
85
Burn
Hut
Temp
PC)
125
96
101
111
114
114
127
96
76
105
102
100
89
84
85
96
Bag-
house
Inlet
Temp
PC)
72
75
80
84
86
97
72
81
77
74
66
61
67
75
02
(%)
20.5
21.0
20.7
20.7
20.7
20.7
20.6
21.3
20.9
20.8
20.9
21.1
20.8
21.1
20.9
21.0
CO2
(%)
0.90
0.23
0.83
0.80
0.77
0.79
0.90
0.75
0.74
0.75
0.69
0.78
0.63
0.71
0.67
0.67
CO
(ppm)
176
159
101
160
263
302
516
338
145
118
131
147
302
432
412
521
THC
(ppm)
78
56
32
69
103
126
259
169
44
44
37
43
176
263
216
329
Note: Averages for wood feeding started after two wood feed intervals and continued until the first bird feed event
Note: Averages for birds started at first bird feed event and continued until one bird feed interval after the last bird feed event
58
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Wood + 1 Bird/4 min • Wood + 1 Bird/10 min • Wood Only
Baghouse Inlet Temp (°C)
Burn Hut Temp (°C)
Outlet Duct Temp (°C)
Top (Plenum Side) Temp (°C)
Middle (Plenum Side) Temp (°C)
Bottom (Plenum Side) Temp (°C)
Top (Non-Plenum Side) Temp (°C)
Middle (Non-Plenum Side) Temp (°C)
Bottom (Non-Plenum Side) Temp (°C)
0 100 200 300 400 500 600 700 800
Temperature (°C)
Figure 54. Average Temperatures with the ACB under Different Test Conditions
Wood + 1 Bird/2 min
Wood + 1 Bird/4 min
Wood + 1 Bird/6 min
Wood + 1 Bird/8 min
Wood + 1 Bird/10 min
Wood Only
IHC(ppm)
ICO (ppm)
0 100 200 300 400 500 600
Concentration (ppm)
Figure 55. Average CO and THC from Various Test Conditions
59
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
5.3
EXTRACTIVE SAMPLING RESULTS
Table 11 shows the total filterable particulate results for both poultry feed conditions. Although it
is difficult to make a conclusion based on statistical significance due to there only being one
measurement, it appears that the PM emissions, on a concentration basis, at the higher poultry
feed rate are approximately 2.5 times the PM emissions, on a concentration basis, at the lower
poultry feed rate. Although it was not possible to make measurements of visible smoke
emissions (i.e., opacity) inside the Burn Hut, there was a visible difference in emissions between
the two feed conditions, with the higher poultry feed rate resulting in the formation of black
smoke.
Table 11. Total Filterable PM Results [milligrams/dry standard cubic meters (mg/dscm)]
Condition
1 Bird/10 min
1 Bird/4 min
Total Filterable Particulate
19.0
44.6
Table 12 shows the VOC emissions from only the high poultry feed rate test (laboratory
problems with the analysis of the low poultry feed rate test conditions prevented the display of
those data). Note the relative abundance of acetone in the emissions relative to other identified
VOC compounds. The observation of acetone was somewhat consistent with some previous
findings where carboxyl compounds were found at levels generally at or above those of more
conventional combustion-generated VOCs (e.g., benzene) from measurements made grilling
chicken on cookstoves [22].
Table 13 shows the PAH emissions from both poultry feed conditions. Consistent with the PM
results, the PAH emissions from the high poultry feed rate condition (1 bird/4 min) was
approximately two to three times the PAH emissions from the low poultry feed rate conditions (1
bird/10 min). The quantitation on some of the PAH measurements is somewhat questionable
because the concentrations were below the lowest point on the calibration curve for the GC/MS
instrument. Even so, the confidence in the identification of the individual compounds was high.
60
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 12. Volatile Organic Compound Sampling Results (ug/dscm)
Compound
1,3-Butadiene
2-Hexanone
Acetone
Acetonitrile
Acrolein
Benzene
Carbon disulfide
Chloroethane
Chloroform
Chloromethane
Ethanol
Ethyl Acetate
Ethylbenzene
Heptane
Hexane
Isopropyl Alcohol
m-/p-Xylene
Methylene Chloride
o-Xylene
Styrene
Tetrahydrofuran
Toluene
1 Bird/10 min
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
1 Bird/4 min
6.6
ND
286.0
41.8
1.4
2.5
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
2.3
ND
ND
7.8
1.6
J
J
N/A - Not available - Laboratory did not provide QC and
calibration data, so unable to determine reliability of
analytical results
ND - not detected
J - below quantitation level
61
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
T,
able 13. PAH Sampling Results [
Compound
Naphthalene
2-Methylnaphthalene
1-Methylnaphthalene
Acenaphthylene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene
Perylene
lndeno(1,2,3-
cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1 Bird/10 min
268
21.8
16.9
42.1
ND
14.7
52.3
5.16
27.4
22.7
4.34
4.31
5.31
2.16
2.48
3.39
ND
2.44
ND
3.21
parts per billion (ppb)]
j
j
j
j
j
j
j
j
j
j
j
1 Bird/4 min
786
98.0
78.7
198.0
9.7
63.3
151.1
33.32
71.8
58.0
14.21
13.45
14.76
5.38
6.11
10.42
ND
5.52
ND
6.03
J
J
J
J
J
J
J
J
J
J
5.4
ND - not detected
J - below quantitation level
SKC BIOSAMPLER RESULTS
Table 14 shows the results from the SKC Biosamplers for all runs. No viable spores were
identified in the Biosampler (both oil and rinsate) for all runs. This observation suggests that,
within detection limits, no viable spores that were inoculated into the birds survived combustion
and/or became airborne and were released from the Burn Hut into the transition duct and were
captured by sampler. In addition, within detection limits and reflecting observed recoveries for
positive controls, release of pathogens into the air emissions in a form suitable for airborne
transport from an ACB burning poultry carcasses is minimal even at poultry feed rates that
produced a significant deterioration in the environmental effectiveness of ACB operation.
62
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 14. Bioaerosol Sampling Results
Test
Biosampler
Tesfl
Biosampler
Test 2
Biosampler
Test3
Biosampler
Test 4
Biosampler
Tests
Biosampler
Test6
Biosampler
Test?
Biosampler
Tests
Run Date
2/23/201 1
2/28/201 1
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
CPU
in
Oil
0
0
0
0
0
0
0
0
CPU
in
Rinse
0
0
0
0
0
0
0
0
Volume
Plated
(mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
1
1
Oil
Extraction
Volume
(mL)
13.0
11.5
12.0
11.5
12.5
12.5
12.5
12.5
Rinse
Extraction
Volume
(mL)
100
100
100
199
100
100
100
100
CPU
Captured
0
0
0
0
0
0
0
0
m3
Sampled
0.92
0.77
0.81
0.83
0.91
0.83
0.84
0.90
m3/
min
in
Duct
1,209
1,231
1,207
1,217
1,269
1,278
1,224
1,260
CPU
Fed
100000
80000
80000
80000
165000
180000
180000
180000
CFU/m3
0
0
0
0
0
0
0
0
CPU
Recovered
OE+00
OE+00
OE+00
OE+00
OE+00
OE+00
OE+00
OE+00
CPU
Emitted/CPU
Fed
0
0
0
0
0
0
0
0
63
-------
5.5
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
RESIDUAL SPORES IN ASH AND BONES
Table 15 lists the results for the viable spore analysis of the ash that left the ACB but was of a
larger particle nature. This material did not get entrained into the exhaust duct but fell back to
the floor of the Burn Hut. For most of the runs, there were no CPU identified in the cultures. A
number of tests (4 out of 16) did show low but detectable levels of CPU in the floor sweepings
(below quantitation level), suggesting that it is possible that, under some conditions, spores can
leave the ACB bound on (or possibly carried along with) the larger particles that would settle to
the ground in close proximity to the ACB. The reliability of viable spore measurement results
below the CPU cutoff criteria established in the methods [20] is not well-defined.
Table 15. Residual Spores in Fly Ash (Floor Sweeping
Test
Biosampler Test 1a
Biosampler Test 1 b
Biosampler Test 2a
Biosampler Test 2b
Biosampler Test 3a
Biosampler Test 3b
Biosampler Test 4a
Biosampler Test 4b
Biosampler Test 5a
Biosampler Test 5b
Biosampler Test 6a
Biosampler Test 6b
Biosampler Test 7a
Biosampler Test 7b
Biosampler Test 8a
Biosampler Test 8b
Run Date
2/23/201 1
2/23/201 1
2/28/201 1
2/28/201 1
3/2/201 1
3/2/201 1
3/8/201 1
3/8/201 1
3/10/2011
3/10/2011
3/14/2011
3/14/2011
3/16/2011
3/16/2011
3/21/2011
3/21/2011
CPU in
Flyash
0
0
0
0
0.7J
0
0.3J
0.3J
0
0
0
0
0
1.0J
0
0
Volume
Plated
(mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
0.1
1
1
1
1
1
1
1
1
0.1
1
1
Extraction
Volume
(mL)
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
40
s)
Mass of Ash
Collected
(9)
N/A
N/A
1.04
1.57
1.25
1.54
1.70
1.39
1.16
1.41
1.35
1.28
1.74
1.67
2.03
1.75
CFU/g
Flyash
0
0
0
0
2133J
0
78J
96J
0
0
0
0
0
2395J
0
0
J - below quantitation level
Table 16 shows the analytical results for residual viable spores in the poultry bones remaining in
the ACB after the burn. A small amount of viable Gs spores (below quantitation level) was
found on one out of eight samples.
64
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 16. Residual Spores in Poultry Bones
Test
Biosampler
Testl
Biosampler
Test 2
Biosampler
Test3
Biosampler
Test 4
Biosampler
Tests
Biosampler
Test6
Biosampler
Test?
Biosampler
Tests
Run Date
2/23/2011
2/28/2011
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
CPU in
Bone
ND
ND
ND
ND
ND
ND
14J
ND
Volume
Plated (mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
0.1
1
Extraction
Volume
(mL)
25
20
20
20
20
20
20
20
Mass of
Bone
Collected
(g)
3.13
3.17
4.11
2.05
2.80
4.10
3.45
2.35
CFU/g
Bone
ND
ND
ND
ND
ND
ND
8116J
ND
J - below quantitation level
ND - not detected
Table 17 shows the viable residual Gs spores in the ash residue remaining in the ACB following
the burn. No viable spores were found in any of those samples, suggesting that, within
measurement limits, using operational procedures similar to procedures used in these tests (i.e.,
1) startup with fuel and no poultry, 2) operation with poultry and fuel, 3) cessation of poultry
while maintaining operation with fuel only, 4) cool-down), complete inactivation of spores occurs
in whatever materials remain inside the ACB chamber.
65
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 17. Residual Spores in ACB Bottom Ash
Test
Biosampler
Testl
Biosampler
Test 2
Biosampler
Test3
Biosampler
Test 4
Biosampler
Tests
Biosampler
Test6
Biosampler
Test?
Biosampler
Tests
Run Date
2/23/2011
2/28/2011
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
CPU in
Bottom
Ash
0
0
0
0
0
0
0
0
Volume
Plated (mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
1
1
Extraction
Volume
(mL)
20
20
20
20
20
20
20
20
Mass of
Bottom
Ash
Collected
(g)
1.23
1.00
1.09
1.05
1.05
0.96
0.88
0.80
CFU/g
Bottom
Ash
0
0
0
0
0
0
0
0
5.6
WIPE SAMPLE RESULTS
Table 18 shows the results from the wipe samples that were taken following each of the
combustion tests where the poultry carcasses were inoculated with G. stearothermophilus.
There were no viable Gs spores found on any of the samples. This observation is consistent
with the results from the SKC Biosampler samples, suggesting that, within measurement
limitations, no measurable quantities of spores were entrained into the exhaust gases leaving
the Burn Hut.
66
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 18. Results from Wipe Samples after Combustion Tests
Test
Extraction
Test #1
Extraction
Test #2
Biosampler
Testl
Biosampler
Test 2
Biosampler
Test3
Biosampler
Test 4
Biosampler
Tests
Biosampler
Test6
Biosampler
Test?
Biosampler
Tests
Run Date
1/20/2011
2/16/2011
2/23/2011
2/28/2011
3/2/201 1
3/8/201 1
3/10/2011
3/14/2011
3/16/2011
3/21/2011
Wipe
Location
W1
W2
W1
W3
W1
W3
W1
W3
W1
W3
W1
W3
W1
W3
W1
W3
W1
W3
W1
W3
CPU
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Volume
Plated
(mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Extraction
Volume
(mL)
20
20
20
20
20
20
20
20
13
13
14
13.5
10
11
9
10
9.5
10.5
10
12
Surface
Area
Sampled
(ft*)
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CFU/ft2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Table 19 shows the results from the wipe samples taken after the Burn Hut interior walls and
floor were decontaminated with Spor-Klenz®. However, there were two sets of wipe samples
(before Biosampler Test 5 and before Biosampler Test 6) that showed low numbers (below the
quantitation limit) of spores. Given that the samples of the walls taken after the runs but before
decontamination all showed no growth of viable spores, it is possible that there may have been
some cross-contamination of the Burn Hut, or maybe, because there were small numbers of
viable spores found in some of the floor sweepings, that they may have been disturbed during
the collection process and deposited on the walls and not been effectively treated with the Spor-
Klenz®.
67
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 19. Results from Wipe Samples after Burn Hut Decontamination
Test
Before
Biosampler
Testl
Before
Biosampler
Test 2
Before
Biosampler
Test3
Before
Biosampler
Test 4
Before
Biosampler
Tests
Before
Biosampler
Test6
Before
Biosampler
Test?
Before
Biosampler
Tests
Run Date
2/22/2011
2/24/2011
3/1/2011
3/4/2011
3/10/2011
3/14/2011
3/16/2011
3/18/2011
Wipe
Location
W1
W2
W3
W2
W4
W2
W4
W2
W4
W2
W4
W2
W4
W2
W4
W2
W4
CPU
0
0
0
0
0
0
0
0
0
0
26 J
25 J
68 J
0
0
0
0
Volume
Plated
(mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Extraction
Volume
(mL)
10
10
10
20
20
13
13
12
13
13
1
10
9
1
1
1
1
Surface
Area
Sampled
(fr)
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
CFU/ft2
0
0
0
0
0
0
0
0
0
0
260 J
2500 J
6120 J
0
0
0
0
J - Below Quantitation Limit
5.7 POSITIVE CONTROL TESTS
The final set of data involved performing a series of tests to verify that had the spores escaped
the ACB, they would be entrained into the Burn Hut, and either exit the Burn Hut in the exhaust
gas duct and subsequently be captured in the SKC Biosampler or else be deposited on the
walls of the Burn Hut. The results from these experiments are shown in Tables 20 and 21. The
following was used for the inoculum: 1.74 mL of sterile 200 proof ethanol, 4.26 mL of sterile
deionized (Dl) water, 21.6 uL of 2.8E8 CFU/mL Geobacillus stearothermophilus solution, and 14
mL of sterile water (to bring the spore solution to -20 mL. A collision nebulizer with precious
fluids bottle (Model CN25/CN40, BGI Incorporated, Waltham, Massachusetts, USA) was used
for all positive control tests. The nebulizer is a three-jet model that requires a source of clean
compressed air to nebulize the solution. The stem of the nebulizer was placed below the liquid
level and the solution was nebulized through small holes on the section of the stem above the
liquid level. Clean compressed air was supplied to the nebulizer at 50 psi for the duration of all
positive control tests. The end of the 11/21/11 test revealed that all the fluid had been used,
and no fluid remained at the end of the test. However, small amounts of residual fluid remained
at the end of other tests. For calculation purposes however, it was assumed that each whole
suspension was sprayed in using the nebulizer, a total of approximately 2E6 CPU of Gs was
sprayed in for each positive control experiment. The first positive control experiment involved
spraying spore inoculum directly into the Burn Hut exit duct, with no combustion occurring in the
68
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
ACB. Viable spores were found on some of the wipe samples and in the SKC Biosamplers.
Even a small number of spores captured in a sampler still can translate into large numbers
emitted from the duct due to high flow rate and small sample volume. The second positive
control experiment involved spraying spore inoculum directly into the Burn Hut, with no
combustion occurring in the ACB. Small numbers of viable spores (below quantitation limit)
were found on most of the wipe samples and in the SKC Biosamplers. The third positive control
experiment involved spraying spore inoculum directly into the Burn Hut exit duct, with
combustion occurring in the ACB (wood and non-inoculated birds, fed at 1 bird/10 minutes). No
viable spores were found on any of the wipe samples. However, viable spores were found in
the SKC Biosamplers. Recoveries ranged from 43 to 149%, indicating that the SKC
Biosamplers successfully captured the spores from the duct.
Table 20. Results from Positive Controls - Wipe Samples
Test
Positive
Control
Testl
Positive
Control
Test 2
Positive
Control
Test3
Run Date
11/21/2011
11/22/2011
11/29/2011
Sample
W1
W2
W3
W4
W1
W1
W3
W4
W1
W2
W3
W4
CPU
0.00
0.00
0.00
0.33 J
0.00
0.67 J
0.33 J
0.33 J
0.00
0.00
0.00
0.00
Volume
Plated
(mL)
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
Extraction
Volume
(mL)
20
20
20
20
20
20
20
20
20
20
20
20
Surface
Area
Sampled
(fn
1
1
1
1
1
1
1
1
1
1
1
1
CPU/ft2
0
0
0
67 J
0
133 J
67 J
67 J
0
0
0
0
J - Below Quantitation Limit
69
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
Table 21. Results from Positive Controls - SKC Biosamplers
Test
Positive
Control
Testl
Positive
Control
Test 2
Positive
Control
Test3
Run Date
11/21/2011
11/22/2011
11/29/2011
CPU
in Oil
5.7 J
3.3 J
1.7J
CPU
in
Rinse
0
0
0
Volume
Plated
(mL)
0.1
0.1
0.1
Dilution
Factor
1
1
1
Oil
Extraction
Volume
(mL)
20.0
20.0
20.0
Rinse
Extraction
Volume
(mL)
40
20
40
CPU
Captured
1133
667
333
m3
Sampled
0.81
0.78
0.74
m3
/min
in
Duct
36
38
32
CPU Fed
2E+06
2E+06
2E+06
CFU/m3
1391
854
450
CPU
Recovered
3E+06
2E+06
9E+05
Recovery
%
149%
96%
43%
J - Below Quantitation Limit
70
-------
5.8
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
ESTIMATED EMISSIONS IN EMISSION FACTOR UNITS
A useful way to compare emissions from combustion devices over a wide range of sizes and
designs is to calculate the emissions in terms of emission factor (EF) units, which typically
normalize emissions per unit mass of fuel fed to the process. Equation (1) shows the formula to
calculate the estimated emissions in EF units.
EF =
C,Qtotal
m
(1)
feed
where EF is the emission factor in units of mass emitted per mass of fuel burned; d is the
concentration of pollutant i, Qtotai is the total volumetric flow rate of flue gases, and mfeed is the
mass feed rate of the fuel being burned.
Table 22 shows the estimated emissions of various pollutants in EF units. Note that the column
with the heading I PAH represents the sum of all identified PAH species. The PM EF for trench
burners reported in the EPA's AP-42 Emission Factor Database [23] is 6,500 mg/kg fuel burned,
which is similar in magnitude to the EF from the pilot-scale ACB burning wood with poultry. The
CO EF for ACBs burning woody debris was 5,000 mg/kg fuel burned [3], which is approximately
half the CO emissions from the pilot-scale ACB burning wood only. There are no published EFs
forTHCsandPAHs.
Table 22. Estimated Emissions of Various Pollutants in Emission Factor Units
Condition
Wood
Only
Wood + 1
Bird/10
min
Wood + 1
Bird/4 min
CO(ppm)
55
140
401
THC
(ppm)
12
45
231
PM
(mg/m3)
NA
19
45
ZPAH
(Mg/m3)
NA
499
1623
CO
(mg/kg
total fuel)
11,500
18,300
33,900
THC
(mg/kg
total fuel)
3,900
9,200
30,700
PM
(mg/kg
total fuel)
NA
2,200
3,300
ZPAH
total fuel)
NA
57,200
119,900
NA - not applicable
71
-------
Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
6.0 QUALITY CONTROL EVALUATION REPORT
6.1 CEM AND THERMOCOUPLE DATA
The thermocouples used in this testing were new before being installed in the ACB. They
arrived from the factory with certified calibrations. Two thermocouples in the ACB failed during
the testing, but these were replaced with new units. All the temperature measurements were in
agreement with previous testing and therefore are deemed to have met the criteria for critical
measurements of ±5 °C. The thermocouples used for the sampling trains are also calibrated by
the U.S. EPA Metrology Laboratory and have certified calibration sheets associated with each of
them.
The 100% completeness criterion was met for the Burn Hut exhaust velocity traverses,
volumetric flow rates, and exhaust moisture content measurements. In addition, the two meter
boxes used for the volumetric flow rate were calibrated prior to sampling and following sampling
with acceptable results.
The CEMs used in this testing were CO2/O2, low and high CO, and THC. Each day of testing
did not proceed until these instruments were successfully calibrated with protocol calibration
gases. In addition, a bias check was run at the end of each test to check for drift and to verify
instrument operation throughout the test.
6.2 PM DATA
An accredited laboratory (Resolution Analytics, Inc., of Sanford, NC) was used for the analyses
of filterable particulate (PM) from the two extraction tests conducted on 1-20-2011 and 2-16-
2011. EPA Method 5 was used and a complete analytical report was provided for each event.
6.3 VOC DATA
After the first set of sample results were returned from the analytical laboratory, it was
discovered that their report contained nothing but the results. Upon being contacted to provide
their QC and calibration data, the laboratory stated they did not have any backup to provide.
The decision was made to cease using this laboratory for any future testing done on this project.
Without any QC or calibration data, it is impossible to judge the quality of the VOC results from
the baseline test. The data from the 1-20-11 tests are therefore not reported.
An accredited laboratory was used for the 2-16-11 tests with the high poultry feed rate. This
laboratory provided a complete and consistent Level IV report. QC data, calibrations and
chromatograms were provided, and there seems to be no cause to question the validity of their
results. The 5.000X dilution (and subsequent 10.000X dilution to bring acetone within the
calibration range of the instrument) was required to meet the client-requested 1 ppmv reporting
limit. Because the results must be multiplied by these dilution factors, small errors do become
magnified.
6.4 PAH DATA
An accredited laboratory (Enthalpy Analytical, Inc. of Durham, NC) was used for the PAH
analysis of the two extraction tests conducted on 1-20-2011 and 2-16-2011. EPA Method
8270D [16] was used and a complete analytical report was provided for each event.
6.5 SKC BIOSAMPLER DATA
All SKC Biosamplers were processed through the BL according to established Miscellaneous
Operating Procedures (MOPs). All QAPP acceptance criteria were met pertaining to data
collection and how spores were enumerated for the SKC Biosampler exhaust samples, as well
as for those processed from the floor sweeping HEPA vacuum samples, the sterile wipes used
72
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
on the walls of the Burn Hut, and from the bulk ash samples.
6.6 BLANKS, POSITIVE CONTROLS, AND NEGATIVE CONTROLS
The positive and negative controls from the bird homogenates could not be analyzed. The mass
of debris simply overwhelmed the plates and made the identification and enumeration of spores
impossible. All other blanks such as the wipe blanks and field sampling blanks were collected
as planned. Recoveries from the positive controls performed during November 2011 that
involved injection of the inoculum into the Burn Hut and Burn Hut duct were within an
acceptable range.
6.7 SUM MARY OF QC OBSERVATIONS
All data were collected with the deviations noted above, except for the RTP Laboratories VOC
data which were not reported as useable due to lack of QC and are considered unuseable for
the purposes of this study.
73
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
7.0 CONCLUSIONS
Tests were run on a pilot-scale ACB burning a combination of wood and poultry carcasses. The
tests were run to evaluate potential emissions of pathogens (using spores of a nonpathogenic
bacterium as a surrogate pathogen) as a function of degrading system combustion efficiency
due to overcharging of poultry carcasses. The objective of the tests was to evaluate whether
pathogens might be released from an ACB system as air emissions or in ash if the performance
of the ACB began to degrade due to operational issues, or whether effective pathogen
destruction still occurred in spite of potentially increased air emissions from the ACB due to
operational issues. If environmental performance degrades prior to a reduction in complete
pathogen destruction, then it is likely that operational adjustments and corrective actions could
be made based on easily observable increases in visible emissions without an increased risk of
pathogen release, provided corrective action is taken quickly. Although the nature of the test
facility prevented the quantitative measurement of visible emissions using opacity measurement
methods, there was significantly more black smoke formed during the high poultry feed rate
conditions.
7.1 COMPARISON OF PILOT-SCALE ACB EMISSIONS TO FULL-SCALE ACB
EMISSIONS
Several observations were noted:
• The estimated emissions of PM in emission factor units when poultry combustion was
occurring compared favorably to published PM emission factors for ACBs burning vegetative
debris;
• The estimated emissions of CO in emission factor units when poultry combustion was
occurring were approximately a factor of two higher than published CO emission factors for
ACBs burning vegetative debris; and
• These two observations suggest that the overall operation of the pilot-scale ACB effectively
mimicked a much larger full-scale ACB.
7.2 EFFECT OF BURNING POULTRY ON EMISSIONS
• The addition of poultry at the baseline feed rate (1 bird/10 min) to the combustion of wood in
the pilot-scale ACB increased the emissions of CO and THC by a factor of approximately
2.5; and
• The increase in emissions in going from 1 bird every 10 minutes to 1 bird every 4 minutes
increased emissions by approximately another factor of 2.5.
7.3 EMISSION OF VIABLE SPORES FROM COMBUSTION OF CONTAMINATED
POULTRY
• Viable spores were not detected in the air emissions in any of the SKC Biosampler runs.
Positive control experiments showed that if not destroyed in the ACB, spores could be
detected in the ACB exhaust duct using the SKC Biosamplers, albeit at a low concentration;
• Small amounts of viable spores were measured in the residual poultry bones in one of the
eight runs;
• Small amounts of viable spores were measured in the floor sweepings of 50% of the runs at
the low poultry feed rate condition and in the floor sweepings of 25% of the runs at the high
poultry feed rate condition;
74
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
• Viable spores were not detected in the residual ash left behind in the ACB for any of the
runs; and
• Viable spores were not detected in any of the post-combustion wipe samples of the Burn
Hut walls.
7.4 SUMMARY
Overall these observations suggest that:
• There does not appear to be an observable difference between the residual viable spore
results at the two test conditions. All test conditions resulted in no measureable viable
spores in the exhaust gases, residual bottom ash, or on the post-combustion wall wipe
samples. The floor sweeping samples showed positive spore counts on occasional
samples, but there was not a difference that was statistically quantifiable; and
• Within the limitations of the experimental apparatus and associated detection limits, it does
not appear that measurable quantities of viable spores were released from combustion of
the inoculated poultry even at poultry feed rates that resulted in pollutant emissions at least
a factor of five higher than the baseline ACB emissions while burning wood only.
7.5 RECOMMENDATIONS FOR FUTURE WORK
Based on some of the limitations of the experiments and analytical methods, if future follow-on
work were to be done the following recommendations are made:
• Use an inoculum with at least a factor of ten higher concentration of spores; and
• Operate the SKC Biosamplers for at least two hours;
75
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Combustion of Contaminated Animal Carcasses in a Pilot-Scale Air Curtain Burner
8.0 REFERENCES
1. U.S. Government, T.W.H. Homeland Security Presidential Directive 9: Defense of United States
Agriculture and Food. 2004 [cited 2013 May 30]; Available from:
http://www.aphis.usda.gov/animal health/emergency management/downloads/hspd-9.pdf.
2. Kansas State University. Carcass Disposal: A Comprehensive Review. 2004 [cited 2013 May
29]; Available from: http://hdl.handle.net/2097/662.
3. Miller, C.A. and P. Lemieux, Emissions from the Burning of Vegetative Debris in Air Curtain
Destructors. Air and Waste Management Association Journal, 2007. 57: p. 959-967.
4. Lemieux, P., Emissions of Libby Amphibole Asbestos from the Simulated Open Burning of Duff,
2012.
5. Air Burners LLC. S-327 Refractory Walled Air Curtain Burner. 2009 [cited 2009 October 23];
Available from: http://www.airburners.com/ab-s327.htm.
6. U.S. EPA, EPA Test Method 3A, Determination of Oxygen and Carbon Dioxide Concentration in
Emissions from Stationary Sources (Instrument Analyzer Procedure), 1989: Washington, DC.
7. U.S. EPA, EPA Test Method 10, Determination of Carbon Monoxide Emissions from Stationary
Sources, 1996: Washington, DC.
8. U.S. EPA, EPA Test Method 25A, Determination of Total Gaseous Organic Concentration Using
Flame lonization Analyzer, 1996: Washington, DC.
9. U.S. EPA, EPA Test Method 1, Sample and Velocity Traverses for Stationary Sources, 1996.
10. U.S. EPA, EPA Test Method 2C, Determination of Stack Gas Velocity and Volumetric Flow Rate
(Type-S Pitot Tube), 1996.
11. U.S. EPA, EPA Test Method 4, Determination of Moisture Content in Stack Gases, 1995:
Washington, DC.
12. U.S. EPA, EPA Test Method 5, Determination of Particulate Emissions from Stationary Sources,
1996: Washington, DC.
13. Tufts, J. and J. Rosati, Thermal Inactivation of Bacillus anthracis Surrogate Spores in a Bench-
Scale Enclosed Landfill Gas Flare. AWMA J., 2012. 62: p. 151-159.
14. Wood, J., et a I., Dry Thermal Resistance of Bacillus anthracis Sterne and other Bacillus Species;
Implications for Biological Agent Destruction via Waste Incineration. Journal of Applied
Microbiology, 2009. 109(1): p. 99-106.
15. U.S. EPA, EPA Test Method 0010, Modified Method 5 Sampling Train, in Test Methods for
Evaluating Solid Wastes, Volume II, 1986: Washington, DC.
16. U.S. EPA. EPA Test Method 8270D, Semivolatile Organic Compounds by Gas
Chromatography/Mass Spectrometry (GC/MS). 2007 September; Available from:
http://www.epa.gov/wastes/hazard/testmethods/sw846/pdfs/8270d.pdf.
17. U.S. EPA, EPA Test Method 0040, Sampling of Principal Organic Hazardous Constituents from
Combustion Sources Using Tedlar® Bags, 1996.
18. U.S. EPA, Assessment of Liquid and Physical Decontamination Methods for Environmental
Surfaces Contaminated with Bacterial Spores: Evaluation of Spray Method Parameters and
Impact of Surface Grime, 2012.
19. U.S. EPA, Evaluation of Expedient Decontamination Options with Activated Peroxide-based
Liquid Sporicides, 2013.
20. Brown, G.S., et al., Evaluation of a Wipe Surface Sample Method for Collection of Bacillus
Spores from Nonporous Surfaces. Applied Environmental Microbiololgy, 2006. 73(3): p. 706-710.
21. U.S. EPA, EPA Compendium Method TO-15, Determination of Volatile Organic Compounds
(VOCs) in Air Collected in Specially-Prepared Canisters and Analyzed by Gas
Chromatography/Mass Spectrometry (GC/MS), 1999.
22. U.S. EPA, Emissions from Street Vendor Cooking Devices (Charcoal Grilling), 1999.
23. U.S. EPA. AP-42 Emission Factor Database. 1995 [cited 2009 October 27]; Available from:
http://www.epa.gov/ttn/chief/ap42/index.html.
76
-------
APPENDIX A: ISOKINETIC SAMPLING WORKSHEETS
A-1
-------
1-20-11 M5.XLS
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2 %
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
US EPA
Air Curtain Burner Outlet Duct
RTP, NC
1R-ACB-PM-01
1/20/2011
1042-1202
150 <-input
1.3 <-input
64 <-input
2 <-input
0.75 <-input
31.01 <-input
1.72 <-input
-2.8 <-input
0.84 <-input
20.9 <-input
0 <-input
0.206 <--CALCULATED
0.208 <-input
1.58 <--CALCULATED
5 <-input
0.894 <-input
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
8 Rd Area:=> 0.35
RecArea:=> 0.00
0
0
Area Used 0.349066
md=
Ps=
Mfd=
Ms=
28.84
30.80
0.980
28.62
REMEMBER TO INPUT YOUR DELTA P AND STACK TEMPERATURE
Sample
Point
1
Stop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Averages~>
Pilot
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.30
Dry Gas
Meter Reading
478.127
483
488.33
493.1
497.7
502.5
507
512.4
516.63
520.95
525.28
529.57
534
Delta H
2.06
2.06
2.01
1.98
2.08
2.03
2.09
2.07
2.11
2.00
2.02
1.99
2.04
Flue Gas
Temp.
166
171
181
150
169
152
158
146
180
174
181
178
167.17
Meter
Temp.
Outlet
71
70
71
71
74
75
75
75
75
74
73
73
73
BELOW
% ISO
97.4
130.7
107.5
101.1
106.6
98.4
118.7
92.0
96.6
96.5
96.3
99.2
103.43
Volume
Metered
4.373
5.830
4.770
4.600
4.800
4.500
5.400
4.230
4.320
4.330
4.290
4.430
4.656
Volume
Metered
Standard
4.047
5.406
4.414
4.256
4.418
4.133
4.961
3.886
3.969
3.984
3.955
4.084
Velocity
(vs)
69.0
69.3
69.8
68.1
69.2
68.2
68.6
67.9
69.8
69.4
69.8
69.7
69.06
Square
Root
Delta P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.14
K-factor
1.58
1.55
1.53
1.60
1.56
1.61
1.59
1.63
1.54
1.55
1.53
1.54
TOTAL VOLUME =
55.873
Run 1
A-2
-------
•20-1 1 M5.XLS
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
US EPA
Air Curtain Burner Outlet Duct
RTF, NC
Particulate and Metals Test Results
1R-ACB-PM-01
1/20/2011
1042-1202
0.894
2.04
31.01
55.873
73
-2.80
167
0.00
0.0
20.9
79.1
0.84
1.140
60.0
0.208
0.000235969
51.504
30.80
1.7
37.0
0.000
0.983
28.84
28.65
69.0
0.35
1,446
1,231
103.2
Results
A-3
-------
1-20-11 M5.XLS
Distance from far wall to outside of port 75.75
Nipple length and/or wall thickness 7.25
Depth of stack or duct 68.5
% of depth
2.1
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
distance
from inside
wall
1.44
4.59
8.08
12.12
17.13
24.39
44.11
51.38
56.38
60.42
63.91
67.06
distance
including
nipple*
8.69
11.84
15.33
19.37
24.38
31.64
51.36
58.63
63.63
67.67
71.16
74.31
* mark these points on probe
M1 Points A-4
-------
2-16-11 M5.XLS
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2 %
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
US EPA
Air Curtain Burner Outlet Duct
RTP, NC
1R-ACB-PM-02
2/16/2011
1016-1118
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
8 Rd Area:=> 0.35
RecArea:=> 0.00
0
0
Area Used 0.349066
150
1.4
66
2
0.75
30.06
1.72
-2.8
0.84
20.9
0
<-input
<-input
<-input
<-input
<-input
<-input
<-input
<-input
<-input
<-input
<-input
md=
Ps=
Mfd=
Ms=
28.84
29.85
0.980
28.62
0.201 <--CALCULATED
0.208 <-input
1.59 <--CALCULATED
5 <-input
0.894 <-input
REMEMBER TO INPUT YOUR DELTA P AND STACK TEMPERATURE BELOW
Sample
Point
1
Stop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Averages~>
Pilot
Reading
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.40
Dry Gas
Meter Reading
535.954
541
544.995
549.45
554.02
558.67
563.15
567.695
572.34
576.95
581.05
585.195
588.007
Delta H
2.22
2.22
2.19
2.21
2.24
2.19
2.11
2.21
2.10
2.19
2.20
2.23
2.19
Flue Gas
Temp.
141
158
152
142
157
181
152
187
158
155
149
218
162.50
Meter
Temp.
Outlet
66
65
64
64
64
64
65
66
66
66
66
67
65
% ISO
95.5
95.1
94.4
96.1
99.0
97.2
96.1
100.8
97.8
86.8
87.3
62.4
Volume
Metered
4.566
4.475
4.455
4.570
4.650
4.480
4.545
4.645
4.610
4.100
4.145
2.812
Volume
Metered
Standard
4.137
4.063
4.052
4.157
4.230
4.075
4.125
4.209
4.176
3.715
3.756
2.543
Velocity
(vs)
71.3
72.3
71.9
71.3
72.2
73.6
71.9
73.9
72.3
72.1
71.7
75.7
Square
Root
Delta P
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
K-factor
1.59
1.56
1.58
1.60
1.56
1.51
1.58
1.50
1.57
1.57
1.59
1.43
92.36
4.338
1.18
TOTAL VOLUME =
52.053
Run 1
A-5
-------
•16-11 M5.XLS
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
US EPA
Air Curtain Burner Outlet Duct
RTF, NC
Particulate Test Results
1R-ACB-PM-02
2/16/2011
1016-1118
0.894
2.19
30.06
52.053
65
-2.80
163
18.03
0.0
20.9
79.1
0.84
1.183
60.0
0.208
0.000235969
47.231
29.85
1.8
34.2
0.850
0.982
28.84
28.64
72.5
0.35
1,518
1,262
92.3
Results
A-6
-------
2-16-11 M5.XLS
Distance from far wall to outside of port 75.75
Nipple length and/or wall thickness 7.25
Depth of stack or duct 68.5
% of depth
2.1
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
distance
from inside
wall
1.44
4.59
8.08
12.12
17.13
24.39
44.11
51.38
56.38
60.42
63.91
67.06
distance
including
nipple*
8.69
11.84
15.33
19.37
24.38
31.64
51.36
58.63
63.63
67.67
71.16
74.31
* mark these points on probe
M1 Points A-7
-------
biosampler train_2-23-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facilily Name:
Sampling Location:
Cily, Slale:
Operator's Initials:
Run Number:
Tesl Dale:
Run Time:
K Factor Selup
Slack Temp.
Average Delia P
Meier Temp.
% Moislure
Sample Rale
Baromelric Pres.
Delia H@
Slalic Pressure
Pilol Coefficienl
O2%
CO2 %
Desired Nozzle
Aclual Nozzle
K Factor
Minules/Poinl
Meier Box Gamma
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
Dry Gas
Meier Reading
480.63
483.24
485.9
488.56
491.14
493.74
496.33
498.9
501.46
504
506.53
509.04
511.542
USEPA Slack Diameter (Rd):
ACB Outlet Duel Slack Dimension (Rec):
RTP, NC Widlh
jtn Deplh
2-ACB-BS-01
2/23/201 1
1040-1140
156<-inpul
1.3 <~input
60 <~input
2 <~input
0.44 <~input
30.21 <~input
1.88 <~input
-1.8 <~input
0.84 <~input
20.9 <~input
0 <~input
0.157<-CALCULATED
= 0.157 <~input
0.55 <~CALCULATED
= 5 <— inpul
1.021 <~input
Delia H
0.72
0.72
0.71
0.71
0.71
0.70
0.68
0.69
0.70
0.70
0.69
0.69
Flue Gas
Temp.
156
158
164
161
166
183
179
175
175
179
186
182
Outlet
Meter
Temp.
57
57
57
57
57
57
58
59
60
60
61
61
% ISO
116.7
119.1
119.7
115.8
117.2
118.3
116.8
115.8
114.7
114.6
114.1
113.3
8
0
0
Volume
Metered
2.610
2.660
2.660
2.580
2.600
2.590
2.570
2.560
2.540
2.530
2.510
2.502
Rd Area:=>
Rec Area:=>
Area Used
Volume
Melered
Slandard
2.752
2.804
2.804
2.720
2.741
2.730
2.704
2.688
2.662
2.652
2.626
2.617
0.35
0.00
0.349066
Velocily
(vs)
69.3
69.4
69.7
69.5
69.8
70.8
70.5
70.3
70.3
70.5
70.9
70.7
Square
Rool
Delia P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
md=
Ps=
Mfd=
Ms=
K-faclor
0.55
0.55
0.54
0.54
0.54
0.53
0.53
0.54
0.54
0.53
0.53
0.53
28.84
30.08
0.980
28.62
Averages-> 1.30
0.70
172.00
58.42
116.35
70.16
TOTAL VOLUME =
30.912
Run 1
A-8
-------
bios
implertrain_2-23-11.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H2O)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUN TIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pilot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)1/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight-wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-01
2/23/2011
1040-1140
1.021
0.70
30.21
30.912
58
-1.80
172
0.0
0.0
20.9
79.1
0.84
1.140
60.0
0.157
0.000134439
32.499
30.08
2.0
42.4
0.000
0.980
28.84
28.62
70.2
0.35
1,469
1,209
116.4
Results
A-9
-------
biosampler train_2-23-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-10
-------
biosampler train_2-28-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
2-ACB-BS-02
2/28/2011
1250-1350
170 <-input
1.4 <—input
70 <-input
2 <-input
0.44 <-input
29.5 <-input
1.88 <-input
-1.7 <-input
0.84 <-input
20.9 <-input
0 <-input
0.154<-CALCULATED
0.157 <-input
0.55 <~CALCULATED
5 <—input
1.021 <-input
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
Area Used 0.349066
md=
Ps=
Mfd=
Ms=
28.84
29.38
0.980
28.62
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
Dry Gas
Meier Readint
511.619
513.93
516.24
518.58
520.9
523.16
525.46
527.73
529.95
532.16
534.4
536.59
538.784
Delia H
0.77
0.77
0.77
0.76
0.76
0.75
0.76
0.75
0.77
0.75
0.76
0.76
Flue Gas
Temp.
175
168
182
177
188
180
187
176
194
182
186
173
Outlet
Meier
Temp.
69
69
69
70
70
71
71
72
72
73
73
74
% ISO
97.7
97.1
99.4
98.0
96.3
97.2
96.5
93.4
94.2
94.5
92.6
91.7
Volume
Melered
2.311
2.310
2.340
2.320
2.260
2.300
2.270
2.220
2.210
2.240
2.190
2.194
Volume
Melered
Slandard
2.326
2.325
2.355
2.330
2.270
2.306
2.276
2.221
2.211
2.237
2.187
2.187
Velocily
(vs)
73.8
73.4
74.3
74.0
74.6
74.1
74.5
73.9
74.9
74.3
74.5
73.7
Square
Rool
Delia P
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
K-faclor
0.55
0.55
0.54
0.54
0.54
0.54
0.54
0.55
0.53
0.54
0.54
0.55
Averages-> 1.40
0.76
180.67
71.0
95.71
74.17
1.18
TOTAL VOLUME =
27.165
Run 1
A-11
-------
osampler train_2-28-1 1 .xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-02
2/28/2011
1250-1350
1.021
0.76
29.50
27.165
71
-1.70
181
0.0
0.0
20.9
79.1
0.84
1.183
60.0
0.157
0.000134439
27.228
29.38
2.0
52.7
0.000
0.980
28.84
28.62
74.2
0.35
1,554
1,231
95.7
Results
A-12
-------
biosampler train_2-28-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-13
-------
biosampler train_3-2-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
2-ACB-BS-03
3/2/2011
0850-0950
160
1.3
55
2
0.44
30.31
1.88
-1.7
0.84
20.9
0
<-input
<—input
<—input
<-input
<-input
<—input
<—input
<-input
<-input
<—input
<—input
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
0.159<-CALCULATED
0.157 <-input
0.54 <~CALCULATED
5 <—input
1.021 <-input
Area Used 0.349066
md=
Ps=
Mfd=
Ms=
28.84
30.19
0.980
28.62
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
Dry Gas
Meier Readint
538.726
541.1
543.39
545.7
547.99
550.26
552.52
554.81
557.04
559.25
561.42
563.55
565.666
Delta H
0.71
0.71
0.72
0.69
0.69
0.70
0.69
0.69
0.67
0.68
0.68
0.66
Flue Gas
Temp.
150
148
173
176
166
172
168
189
182
183
199
205
Outlet
Meter
Temp.
56
56
56
53
52
52
52
53
53
54
54
55
% ISO
106.0
102.1
105.1
105.0
103.5
103.5
104.6
103.3
101.8
99.9
99.2
98.8
Volume
Metered
2.374
2.290
2.310
2.290
2.270
2.260
2.290
2.230
2.210
2.170
2.130
2.116
Volume
Metered
Standard
2.516
2.427
2.448
2.441
2.424
2.414
2.446
2.377
2.356
2.308
2.266
2.247
Velocity
(vs)
68.8
68.7
70.1
70.3
69.7
70.0
69.8
71.0
70.6
70.6
71.5
71.8
Square
Root
Delta P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
K-factor
0.54
0.56
0.53
0.53
0.54
0.53
0.53
0.52
0.52
0.52
0.51
0.51
Averages-> 1.30
0.69
175.92
53.83
102.74
70.24
TOTAL VOLUME =
26.94
Run 1
A-14
-------
osamplertrain_3-2-11.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-03
3/2/2011
0850-0950
1.021
0.69
30.31
26.940
54
-1.70
176
0.0
0.0
20.9
79.1
0.84
1.140
60.0
0.157
0.000134439
28.669
30.19
2.0
46.1
0.000
0.980
28.84
28.62
70.2
0.35
1,471
1,207
102.8
Results
A-15
-------
biosampler train_3-2-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-16
-------
biosampler train_3-8-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facilily Name:
Sampling Location:
Cily, Slale:
Operator's Initials:
Run Number:
Tesl Dale:
Run Time:
K Factor Selup
Slack Temp.
Average Delia P
Meier Temp.
% Moislure
Sample Rale
Baromelric Pres.
Delia H@
Slalic Pressure
Pilol Coefficienl
O2%
CO2 %
Desired Nozzle
Aclual Nozzle
K Factor
Minules/Poinl
Meier Box Gamma
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
Dry Gas
Meier Reading
565.777
568.05
570.33
572.6
574.86
577.14
579.41
581.64
583.87
586.1
588.33
590.55
592.713
USEPA Slack Diameter (Rd):
ACB Outlet Duel Slack Dimension (Rec):
RTP, NC Widlh
jtn Deplh
2-ACB-BS-04
3/8/201 1
0930-1030
140 <~input
1.3 <~input
40 <~input
2 <~input
0.44 <~input
30.4 <~input
1.88 <~input
-1.6 <~input
0.84 <~input
20.9 <~input
0 <~input
0.160<-CALCULATED
= 0.157 <~input
0.55 <~CALCULATED
= 5 <— inpul
1.021 <~input
Delia H
0.71
0.71
0.68
0.68
0.68
0.69
0.69
0.69
0.68
0.69
0.67
0.67
Flue Gas
Temp.
160
169
164
163
161
163
161
170
166
184
181
175
Outlet
Meter
Temp.
40
40
41
41
42
43
44
45
45
47
47
48
% ISO
105.7
106.8
105.7
105.2
105.7
105.2
103.0
103.5
103.2
104.3
103.6
100.2
8
0
0
Volume
Metered
2.273
2.280
2.270
2.260
2.280
2.270
2.230
2.230
2.230
2.230
2.220
2.163
Rd Area:=>
Rec Area:=>
Area Used
Volume
Melered
Slandard
2.493
2.501
2.485
2.474
2.491
2.475
2.427
2.422
2.422
2.412
2.401
2.335
0.35
0.00
0.349066
Velocily
(vs)
69.3
69.8
69.5
69.4
69.3
69.4
69.3
69.8
69.6
70.6
70.4
70.1
Square
Rool
Delia P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
md=
Ps=
Mfd=
Ms=
K-faclor
0.55
0.52
0.53
0.53
0.53
0.53
0.53
0.52
0.53
0.52
0.52
0.52
28.84
30.28
0.980
28.62
Averages-> 1.30
0.69
168.08
43.58
104.35
69.70
TOTAL VOLUME =
26.936
Run 1
A-17
-------
osamplertrain_3-8-11.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-04
3/8/2011
0930-1030
1.021
0.69
30.40
26.936
44
-1.60
168
0.0
0.0
20.9
79.1
0.84
1.140
60.0
0.157
0.000134439
29.335
30.28
2.0
38.5
0.000
0.980
28.84
28.62
69.7
0.35
1,460
1,217
104.4
Results
A-18
-------
biosampler train_3-8-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-19
-------
biosampler train_3-10-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facilily Name:
Sampling Location:
Cily, Slale:
Operator's Initials:
Run Number:
Tesl Dale:
Run Time:
K Factor Selup
Slack Temp.
Average Delia P
Meier Temp.
% Moislure
Sample Rale
Baromelric Pres.
Delia H@
Slalic Pressure
Pilol Coefficienl
O2%
CO2 %
Desired Nozzle
Aclual Nozzle
K Factor
Minules/Poinl
Meier Box Gamma
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
Dry Gas
Meier Reading
592.915
595.61
598.23
600.83
603.45
606.06
608.66
611.2
613.73
616.26
618.8
621.26
623.732
USEPA Slack Diameter (Rd):
ACB Outlet Duel Slack Dimension (Rec):
RTP, NC Widlh
jtn Deplh
2-ACB-BS-05
3/10/2011
0912-1012
154 <~input
1.4 <— inpul
40 <~input
2 <~input
0.44 <~input
29.92 <~input
1.88 <~input
-1.9 <~input
0.84 <~input
20.9 <~input
0 <~input
0.158<-CALCULATED
= 0.157 <~input
0.53 <~CALCULATED
= 5 <— inpul
1.021 <~input
Delia H
0.75
0.75
0.78
0.77
0.79
0.81
0.81
0.75
0.78
0.78
0.77
0.74
Flue Gas
Temp.
154
146
151
139
126
125
169
147
152
154
185
175
Outlet
Meter
Temp.
53
53
54
55
55
56
57
57
58
59
59
60
% ISO
116.3
112.3
111.7
111.3
109.6
108.9
110.1
107.7
108.0
108.4
107.6
107.0
8
0
0
Volume
Metered
2.695
2.620
2.600
2.620
2.610
2.600
2.540
2.530
2.530
2.540
2.460
2.472
Rd Area:=>
Rec Area:=>
Area Used
Volume
Melered
Slandard
2.836
2.757
2.731
2.747
2.736
2.721
2.653
2.642
2.637
2.642
2.559
2.566
0.35
0.00
0.349066
Velocily
(vs)
72.1
71.6
71.9
71.2
70.5
70.4
73.0
71.7
72.0
72.1
73.9
73.3
Square
Rool
Delia P
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
md=
Ps=
Mfd=
Ms=
K-faclor
0.53
0.55
0.55
0.56
0.58
0.58
0.54
0.56
0.55
0.55
0.53
0.54
28.84
29.78
0.980
28.62
Averages-> 1.40
0.77
151.92
56.33
109.91
71.!
1.18
TOTAL VOLUME =
30.817
Run 1
A-20
-------
osampler train_3-1 0-1 1 .xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-05
3/10/2011
0912-1012
1.021
0.77
29.92
30.817
56
-1.90
152
0.0
0.0
20.9
79.1
0.84
1.183
60.0
0.157
0.000134439
32.223
29.78
2.0
26.6
0.000
0.980
28.84
28.62
72.0
0.35
1,508
1,269
109.9
Results
A-21
-------
biosampler train_3-10-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-22
-------
biosampler train_3-14-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
2-ACB-BS-06
3/14/2011
0912-1012
150
1.4
40
2
0.44
30.26
1.88
-1.9
0.84
20.9
0
<-input
<—input
<—input
<-input
<-input
<—input
<—input
<-input
<-input
<—input
<—input
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
Area Used 0.349066
md=
Ps=
Mfd=
Ms=
28.84
30.12
0.980
28.62
0.158<-CALCULATED
0.157 <-input
0.54 <~CALCULATED
5 <—input
1.021 <-input
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
Dry Gas
Meier Readint
624.419
626.78
629.09
631.4
633.71
635.99
638.25
640.5
642.73
644.94
647.13
649.28
651.359
Delta H
0.75
0.75
0.76
0.74
0.78
0.76
0.77
0.77
0.78
0.75
0.78
0.72
Flue Gas
Temp.
151
145
160
133
150
140
141
134
153
130
186
170
Outlet
Meter
Temp.
41
41
42
42
43
43
44
45
45
45
46
46
% ISO
104.7
101.9
103.0
100.7
100.6
98.9
98.3
96.7
97.4
94.7
97.0
92.7
Volume
Metered
2.361
2.310
2.310
2.310
2.280
2.260
2.250
2.230
2.210
2.190
2.150
2.079
Volume
Metered
Standard
2.573
2.517
2.512
2.512
2.475
2.453
2.438
2.411
2.390
2.368
2.320
2.243
Velocity
(vs)
71.5
71.2
72.1
70.5
71.5
70.9
70.9
70.5
71.7
70.3
73.6
72.6
Square
Root
Delta P
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
K-factor
0.54
0.54
0.53
0.55
0.54
0.55
0.55
0.56
0.54
0.56
0.51
0.53
Averages-> 1.40
0.76
149.42
43.58
98.87
71.44
1.18
TOTAL VOLUME =
26.94
Run 1
A-23
-------
osampler train_3-1 4-1 1 .xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-06
3/14/2011
0912-1012
1.021
0.76
30.26
26.940
44
-1.90
149
0.0
0.0
20.9
79.1
0.84
1.183
60.0
0.157
0.000134439
29.209
30.12
2.0
24.7
0.000
0.980
28.84
28.62
71.4
0.35
1,496
1,278
98.9
Results
A-24
-------
biosampler train_3-14-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-25
-------
biosampler train_3-16-11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facilily Name:
Sampling Location:
Cily, Slale:
Operator's Initials:
Run Number:
Tesl Dale:
Run Time:
K Factor Selup
Slack Temp.
Average Delia P
Meier Temp.
% Moislure
Sample Rale
Baromelric Pres.
Delia H@
Slalic Pressure
Pilol Coefficienl
O2%
CO2 %
Desired Nozzle
Aclual Nozzle
K Factor
Minules/Poinl
Meier Box Gamma
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
Dry Gas
Meier Reading
651.707
654.08
656.44
658.8
661.15
663.48
665.79
668.06
670.34
672.63
674.94
677.21
679.442
USEPA Slack Diameter (Rd):
ACB Outlet Duel Slack Dimension (Rec):
RTP, NC Widlh
jtn Deplh
2-ACB-BS-07
3/16/2011
0922-1022
160 <~input
1.3 <~input
40 <~input
2 <~input
0.44 <~input
30.12 <~input
1.88 <~input
-1.9 <~input
0.84 <~input
20.9 <~input
0 <~input
0.161 <-CALCULATED
= 0.157 <~input
0.53 <~CALCULATED
= 5 <— inpul
1.021 <~input
Delia H
0.69
0.69
0.71
0.71
0.71
0.68
0.70
0.70
0.70
0.71
0.73
0.68
Flue Gas
Temp.
154
141
143
142
168
152
158
158
150
135
174
176
Outlet
Meter
Temp.
43
43
44
44
44
45
46
46
47
48
49
49
% ISO
108.7
107.0
107.0
106.4
107.8
105.3
103.7
104.2
103.8
103.2
104.5
102.9
8
0
0
Volume
Metered
2.373
2.360
2.360
2.350
2.330
2.310
2.270
2.280
2.290
2.310
2.270
2.232
Rd Area:=>
Rec Area:=>
Area Used
Volume
Melered
Slandard
2.564
2.549
2.545
2.534
2.512
2.486
2.438
2.449
2.454
2.471
2.424
2.383
0.35
0.00
0.349066
Velocily
(vs)
69.3
68.5
68.6
68.6
70.0
69.2
69.5
69.5
69.0
68.2
70.4
70.5
Square
Rool
Delia P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
md=
Ps=
Mfd=
Ms=
K-faclor
0.53
0.55
0.55
0.55
0.53
0.54
0.54
0.54
0.54
0.56
0.53
0.52
28.84
29.98
0.980
28.62
Averages-> 1.30
0.70
154.25
45.67
105.36
69.27
TOTAL VOLUME =
27.735
Run 1
A-26
-------
osampler train_3-1 6-1 1 .xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-07
3/16/2011
0922-1022
1.021
0.70
30.12
27.735
46
-1.90
154
0.0
0.0
20.9
79.1
0.84
1.140
60.0
0.157
0.000134439
29.805
29.98
2.0
28.0
0.000
0.980
28.84
28.62
69.3
0.35
1,451
1,224
105.4
Results
A-27
-------
biosampler train_3-16-11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-28
-------
biosampler train_3-21 -11 .xls
Blue = Input Items
Red = Calculated/Protected Items
Facilily Name:
Sampling Location:
Cily, Slate:
Operator's Initials:
Run Number:
Tesl Dale:
Run Time:
K Factor Selup
Slack Temp.
Average Delia P
Meier Temp.
% Moislure
Sample Rale
Baromelric Pres.
Delia H@
Slalic Pressure
Pilol Coefficienl
O2%
CO2 %
Desired Nozzle
Aclual Nozzle
K Factor
Minules/Poinl
Meier Box Gamma
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
1.4
Dry Gas
Meier Reading
679.842
682.44
685.04
687.59
690.11
692.62
695.2
697.64
700.13
702.62
705.09
707.57
710.047
USEPA Slack Diameter (Rd):
ACB Outlet Duel Slack Dimension (Rec):
RTP, NC Widlh
jtn Depth
2-ACB-BS-08
3/21/2011
0938-1038
160 <~input
1.4 <~input
60 <~input
2 <~input
0.44 <~input
30.34 <~input
1.88 <~input
-1.6 <~input
0.84 <~input
20.9 <~input
0 <~input
0.156<-CALCULATED
= 0.157 <~input
0.55 <~CALCULATED
= 5 <— inpul
1.021 <~input
Delia H
0.77
0.77
0.76
0.77
0.73
0.74
0.77
0.77
0.75
0.77
0.76
0.77
Flue Gas
Temp.
179
164
156
186
179
153
158
174
160
168
164
196
Outlet
Meter
Temp.
56
56
56
56
57
58
58
59
60
61
62
62
% I so
114.5
113.2
110.3
111.6
110.4
110.9
105.3
108.7
107.2
106.9
106.7
109.3
8 Rd Area:=>
Rec Area:=>
0
0
Area Used
Volume
Metered
2.598
2.600
2.550
2.520
2.510
2.580
2.440
2.490
2.490
2.470
2.480
2.477
Volume
Melered
Standard
2.756
2.758
2.705
2.674
2.658
2.727
2.579
2.627
2.621
2.595
2.601
2.598
0.35
0.00
0.349066
Velocily
(vs)
73.0
72.2
71.7
73.4
73.0
71.5
71.8
72.7
71.9
72.4
72.2
74.0
Square
Rool
Delia P
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
1.183
md=
Ps=
Mfd=
Ms=
K-faclor
0.55
0.54
0.55
0.52
0.53
0.55
0.55
0.54
0.55
0.54
0.55
0.52
28.84
30.22
0.980
28.62
Averages-> 1.40
0.76
169.75
58.42
109.59
72.50
1.18
TOTAL VOLUME =
30.205
Run 1
A-29
-------
osampler train_3-21 -1 1 .xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
2-ACB-BS-08
3/21/2011
0938-1038
1.021
0.76
30.34
30.205
58
-1.60
170
0.0
0.0
20.9
79.1
0.84
1.183
60.0
0.157
0.000134439
31.896
30.22
2.0
40.0
0.000
0.980
28.84
28.62
72.5
0.35
1,518
1,260
109.6
Results
A-30
-------
biosampler train_3-21 -11 .xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
% of depth
2.1
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
distance
from inside
wall
0.33
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
distance
including
nipple*
3.83
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
mark these points on probe
M1 Points A-31
-------
11-21-11 biosamplertrain.xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
BS-1
11/21/2011
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
Area Used 0.349066
70 <-input
1.2 <-input
75 <-input
0.5 <-input
0.44 <-input
30.2 <-input
1.77 <-input
-2 <-input
0.84 <-input
20.9 <-input
0 <-input
0.149<-CALCULATED
0.157 <-input
0.64 <~CALCULATED
5 <—input
i <-input
md=
Ps=
Mfd=
Ms=
28.84
30.05
0.995
28.78
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
Dry Gas
Meier Readint
290.233
292.94
295.7
298.48
301.17
303.86
306.61
309.33
312.03
314.74
317.45
320.16
322.844
Delia H
0.77
0.77
0.76
0.76
0.76
0.76
0.76
0.76
0.76
0.76
0.76
0.76
Flue Gas
Temp.
75
75
75
75
75
75
75
75
75
75
75
75
Outlet
Meier
Temp.
74
75
75
75
75
76
76
76
77
77
78
78
% ISO
97.5
99.2
99.9
96.7
96.7
98.6
97.6
96.8
97.0
97.0
96.8
95.9
Volume
Melered
2.707
2.760
2.780
2.690
2.690
2.750
2.720
2.700
2.710
2.710
2.710
2.684
Volume
Melered
Slandard
2.397
2.440
2.457
2.378
2.378
2.426
2.400
2.382
2.386
2.386
2.382
2.359
Velocily
(vs)
61.9
61.9
61.9
61.9
61.9
61.9
61.9
61.9
61.9
61.9
61.9
61.9
Square
Rool
Delia P
1.095
1.095
1.095
1.095
1.095
1.095
1.095
1.095
1.095
1.095
1.095
1.095
K-faclor
0.64
0.63
0.63
0.63
0.63
0.63
0.63
0.63
0.63
0.63
0.64
0.64
Averages-> 1.20
0.76
75.00
76.00
97.48
61.87
1.10
TOTAL VOLUME =
32.611
Run 1
A-32
-------
1-21-11 biosamplertrain.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
BS-1
11/21/2011
0.886
0.76
30.20
32.611
76
-2.00
75
0.0
0.0
20.9
79.1
0.84
1.095
60.0
0.157
0.000134439
28.770
30.05
2.0
2.9
0.000
0.980
28.84
28.62
62.0
0.35
1,299
1,262
98.7
Results
A-33
-------
11-21-11 biosamplertrain.xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
% of depth
2.1
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
distance
from inside
wall
0.33
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
distance
including
nipple*
3.83
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
mark these points on probe
M1 Points A-34
-------
11-22-11 biosamplertrain.xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
BS-2
11/22/2011
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
Area Used 0.349066
65 <-input
1.3 <-input
75 <-input
0.5 <-input
0.44 <-input
30.2 <-input
1.77 <-input
-2.2 <-input
0.84 <-input
20.9 <-input
0 <-input
0.147<-CALCULATED
0.157 <-input
0.64 <~CALCULATED
5 <—input
i <-input
md=
Ps=
Mfd=
Ms=
28.84
30.04
0.995
28.78
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
1.3
Dry Gas
Meier Readint
322.846
325.44
328.1
330.76
333.25
335.81
338.39
340.96
343.53
346.09
348.65
351.23
353.778
Delia H
0.84
0.84
0.83
0.83
0.83
0.83
0.83
0.83
0.83
0.83
0.83
0.83
Flue Gas
Temp.
65
65
65
65
65
65
65
65
65
65
65
65
Outlet
Meier
Temp.
69
69
69
70
70
70
71
71
72
72
72
72
% ISO
89.8
92.0
92.0
86.0
88.4
89.1
88.6
88.6
88.1
88.1
88.8
87.7
Volume
Melered
2.594
2.660
2.660
2.490
2.560
2.580
2.570
2.570
2.560
2.560
2.580
2.548
Volume
Melered
Slandard
2.319
2.378
2.378
2.222
2.284
2.302
2.289
2.289
2.276
2.276
2.294
2.265
Velocily
(vs)
63.8
63.8
63.8
63.8
63.8
63.8
63.8
63.8
63.8
63.8
63.8
63.8
Square
Rool
Delia P
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
1.140
K-faclor
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
Averages-> 1.30
0.83
65.00
70.58
88.93
63.80
TOTAL VOLUME =
30.932
Run 1
A-35
-------
1-22-11 biosamplertrain.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
BS-2
11/22/2011
0.886
0.83
30.20
30.932
71
-2.20
65
0.0
0.0
20.9
79.1
0.84
1.140
60.0
0.157
0.000134439
27.572
30.04
2.0
2.1
0.000
0.980
28.84
28.62
64.0
0.35
1,340
1,325
90.0
Results
A-36
-------
11-22-11 biosamplertrain.xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-37
-------
11-29-11 biosamplertrain.xls
Blue = Input Items
Red = Calculated/Protected Items
Facility Name:
Sampling Location:
City, State:
Operator's Initials:
Run Number:
Test Date:
Run Time:
K Factor Setup
Stack Temp.
Average Delta P
Meter Temp.
% Moisture
Sample Rate
Barometric Pres.
Delta H@
Static Pressure
Pilot Coefficient
O2%
CO2 %
Desired Nozzle =
Actual Nozzle =
K Factor =
Minutes/Point =
Meter Box Gamma =
USEPA
ACB Outlet Duct
RTP, NC
jtn
BS-3
11/29/2011
1400-1500
160
1.14
65
2
0.44
29.87
1.77
-2.2
0.84
20.9
<-input
<—input
<—input
<-input
<-input
<—input
<—input
<-input
<-input
<—input
<—input
Stack Diameter (Rd):
Stack Dimension (Rec):
Width
Depth
! Rd Area:=>
Rec Area:=>
0.35
0.00
0.160<-CALCULATED
0.157 <-input
0.52 <~CALCULATED
5 <—input
i <-input
Area Used 0.349066
md=
Ps=
Mfd=
Ms=
28.84
29.71
0.980
28.62
Sample
Poinl
1
2
3
4
5
6
7
8
9
10
11
12
Slop
Elapsed
Time
0
5
10
15
20
25
30
35
40
45
50
55
60
Pilol
Reading
1.14
1.14
1.14
1.14
1.14
1.14
1.14
1.14
1.14
1.14
1.14
1.14
Dry Gas
Meier Readint
353.778
356.23
358.71
361.17
363.63
366.12
368.59
371.07
373.56
376.04
378.53
381.01
383.469
Delia H
0.59
0.59
0.60
0.61
0.60
0.61
0.59
0.59
0.59
0.60
0.58
0.59
Flue Gas
Temp.
178
160
148
155
151
171
176
178
163
183
174
187
Outlet
Meier
Temp.
68
69
69
70
70
71
71
72
72
72
73
73
% ISO
100.7
100.2
98.4
98.8
99.7
100.3
101.1
101.5
99.9
101.9
100.6
100.8
Volume
Melered
2.452
2.480
2.460
2.460
2.490
2.470
2.480
2.490
2.480
2.490
2.480
2.459
Volume
Melered
Slandard
2.171
2.192
2.174
2.170
2.197
2.175
2.184
2.188
2.179
2.188
2.175
2.157
Velocily
(vs)
66.4
65.5
64.8
65.2
65.0
66.1
66.3
66.4
65.6
66.7
66.2
66.9
Square
Rool
Delia P
1.068
1.068
1.068
1.068
1.068
1.068
1.068
1.068
1.068
1.068
1.068
1.068
K-faclor
0.52
0.53
0.54
0.53
0.53
0.52
0.51
0.51
0.53
0.51
0.52
0.51
Averages-> 1.14
0.60
168.67
70.83
100.33
65.93
1.07
TOTAL VOLUME =
29.691
Run 1
A-38
-------
1-29-11 biosamplertrain.xls
(Y)
(DeltaH)
(Pbar)
(Vm)
(Tm)
(Pg)
(Ts)
(Vic)
(%CO2)
(%O2)
(%N2)
(Cp)
(DeltaP)
(Theta)
(Dn)
(An)
(Vmstd)
(Ps)
(%H20)
(%H2Osat)
(Vwstd)
(Mfd)
(Md)
(Ms)
(Vs)
(A)
(Qa)
(Qs)
(I)
RUN NUMBER
RUN DATE
RUNTIME
MEASURED DATA
Meter Box Y
Avg Delta H, inches H2O
Barometric Pressure, inches Hg
Meter Volume, ft3
Avg Meter Temp, deg F
Static Pressure, inches H2O
Avg Stack Temp, deg F
Water Collected, mL
Carbon Dioxide, %
Oxygen, %
Nitrogen, %
Pitot Tube Coefficient
Avg Sqrt Delta P, (inches H2O)!/2
Sample Time, min
Nozzle Diameter, inches
CALCULATED DATA
Nozzle Area, square feet
Standard Meter Volume, ft3
Stack Pressure, inches Hg
Moisture, %
Moisture (at saturation), %
Standard Water Vapor Volume, ft3
Dry Mole Fraction
Molecular Weight-dry, Ib/lb-mole
Molecular Weight- wet, Ib/lb-mole
Velocity, ft/s
Stack Area, ft2
Volumetric flow, acfm
Volumetric flow, dscfm
Isokinetic Rate, %
USEPA
ACB Outlet Duct
RTF, NC
BioSampler Test Results
BS-3
11/29/2011
1400-1500
0.886
0.60
29.87
29.691
71
-2.20
169
0.0
0.0
20.9
79.1
0.84
1.068
60.0
0.157
0.000134439
26.150
29.71
2.0
39.7
0.000
0.980
28.84
28.62
65.9
0.35
1,381
1,128
100.3
Results
A-39
-------
11-29-11 biosamplertrain.xls
Distance from far wall to outside of port 19
Nipple length and/or wall thickness 3.5
Depth of stack or duct 15.5
distance distance
from inside including
% of depth wall nipple*
2.1 0.33 3.83
6.7
11.8
17.7
25
35.6
64.4
75
82.3
88.2
93.3
97.9
1.04
1.83
2.74
3.88
5.52
9.98
11.63
12.76
13.67
14.46
15.17
4.54
5.33
6.24
7.38
9.02
13.48
15.13
16.26
17.17
17.96
18.67
* mark these points on probe
M1 Points A-40
-------
APPENDIX B: PM AND ORGANIC LABORATORY RESULTS
B-1
-------
RESOLUTION ANALYTICS, INC.
Specialists in Air Emissions Analysis
ANALYTICAL REPORT
CLIENT: ARCADIS, INC.
PROJECT : RN990271.0002- US EPA
ANALYTICAL SERVICES PROVIDED:
FILTERABLE PARTICIPATE
(EPA METHOD 5)
Confirmation of Data Review:
To the best of my knowledge this analytical data has been checked thoroughly for completeness
and the results presented are accurate, error-free, legible, and have been performed and validated
in accordance with the approved method(s).
Date of Review: February 1, 2011
J. Bruce Nemet
Quality Assurance Officer
www.resolutionanalytics.com
2733 Lee Avenue • Sanford, NC 27332 • Phone: 919-774-5557 • Fax: 919-776-6785
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
US EPA
RTP, NC
ACB Outlet Duct
RN99027 1.0002
Resolution Analytics
Sample ID
1R-ACB-PM-01
» --n^^^^M^^tM^^ 1
sSHHiBHRBSBsSiC**1***
f£g.16*fc
fbjfr'fc
Sample Matrix
Dry filter
Front Half Acetone Rinse
~dl
Notes/Comments: |
Analysis Requested
Paniculate
Participate
^la^^T^Ssint^R'^ jt J^LjffsJ
(J^f—
riL>f}T TT~ f* /Jt /fcC^fo
f fr*\r* z^-C'***./** — •" "•• ^
Relinquished By:
Date/Time:
Received By:
Date/Time: ^
'/lA^jtsr /*£?LL
/)-* 7JJ~ // &tt AH*.
//,- /&*£*''.**#&* ^^/v^ x*T-^
y //j&'/j' ^tftttJi*-
Relinquished By: ^^6£^? tf&&C~
Date/Time: fa&f''*t ^f * &
Received By: 'f *?€&&' ^e^dLs
Date/Time: /p 'f-%t/~ If ' ty*f ^ /
/ / / r ' '
ARCAD1S U.S., Inc. Phone Number: (919)544-4535
49 1 5 Prospectus Drive, Suite F Fax Number: (9 1 9) 544-5690
Durham, North Carolina 27713
-------
Client: Arcadis
RESOLUTION ANALYTICS, INC. ^ ^9902710002
Specialists in Air Emissions Analysis Method: EPAM5
Report Summary
SAMPLE ID
Acetone Blank
IR-ACS-PM-01
TOTAL FILTERABLE
PARTICIPATE
0.3 mg (in 230 mis)
27.7 mg
-------
Client: Arcadis
RFA#: RN990271.0002
Specialists in Air Emissions Analysis na ys .
' Analysis: EPA M5
Date Received: 1/24/11
Date Analyzed: 1/24/11
Analyst: JSC
Analysis: EPA M5
Analyte(s): Filterble Participate
Analytical Narrative
Sample Matrix & Components:
Dry Filters, Front/^ Acetone Rinses, Acetone Blank
Summary of Sample Prep:
The acetone rinses were transferred to pre-tared teflon "baggies" in a low humidity environment. The
acetone rinses were evaporated overnight then desiccated for 24 hours, after which time they were weighed
daily every six hours until consecutive weights agreed within ±0.5 mg. The filters were baked 2 to 3 hours
at 105° C, desiccated for 2 hours and weighed.
All weights were recorded to the nearest 0.1 mg and include filterable paniculate catch only. The total
catch reported for each run is a sum of the filter and rinse catches. The acetone blank catch has been
subtracted from sample rinse catches in proportion with their respective volumes.
Summary of Instrumentation:
Denver model Pinnacle Series analytical balance
Analytical Detection Limit(s): 0.1 mg
Miscellaneous Comments Regarding Sample Analysis: (Note unusual catch weights, interferences, odd sample
behavior, and steps taken to confirm unusual results. Also note any deviations from standard analytical
procedures, together with justification and possible affect on results. Specify samples when applicable.)
No modifications to EPA Method 5 analytical procedure were made. See data sheets for individual sample
descriptions.
-------
PARTICULATE SAMPLING LABORATORY RESULTS
Client: Arcadis
Method: EPA M5
Run Number
Filter Container #
Date Init
1/25/11 JSC
Baggie Tare Wt., g.
Filter Tare Wt., g.
FILTER SAMPLE WT., g.
#N/A
Front 14 Rinse Container #
Date Init
1/27/11 JSC F
1/26/11 JSC
Tare Wt., g. ( 40 ml)
RINSE SAMPLE WT., g.
Filter Catch, mg.
Rinse Catch, mg.
Rinse Blank Residue, mg.
Net Rinse Catch, mg.
FILTERABLE PARTICULATE, mg.
RFA#: RN990271.0002
IR-ACS-PM-01 Run 2 Run 3
Date Date
0.3758
#N/A #N/A #N/A
0.3535
0.0223 #N/A ## #N/A #N/A
929
Date Date
3.3650
3.3653 ffl# ##
3.3595 ( ml) #N/A ( ml) #N/A
0.0055 #N/A #N/A
22.3 #N/A #N/A
5.5 #N/A #N/A
0.1 0.0 0.0
5.4 #N/A #N/A
27.7 #N/A #N/A
Legend: F = Final Weight
Notes & Comments:
-------
REAGENT BLANK LABORATORY RESULTS
Client: Arcadis
Method: EPA M5
RFA#: RN990271.0002
Run Number
Acetone Blank
Sample ID/Container #
Date
2273
Init
Tare Wt, g.
SAMPLE WT., g.
Blank Beaker #
Final wt, mg.
Tare wt., mg.
Residue, mg.
Volume, ml.
Density, mg/ml
Cone., mg/mg
Upper Limit, mg
I Legend: F
1/27/11 JSC
1/26/11 JSC F
( 230 ml)
2273
3.5821
3.5818
0.3
230
785.0
1.66E-06 <-
1.00E-05
= Final Weight \
3.5824
3.5821
3.5818
0.0003
Notes & Comments:
-------
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-------
RESOLUTION ANALYTICS, INC.
Specialists in Air Emissions Analysis
ANALYTICAL REPORT
CLIENT: ARCADIS
PROJECT: RN990271.0002
ANALYTICAL SERVICES PROVIDED:
FILTERABLE PARTICIPATE
(EPA METHOD 5)
Confirmation of Data Review:
To the best of my knowledge this analytical data has been checked thoroughly for completeness
and the results presented are accurate, error-free, legible, and have been performed and validated
in accordance with the approved method(s).
Date of Review: March 3,2011
J. Bruce Nemet
Quality Assurance Officer
www.resolutionanalytics.com
2733 Lee Avenue • Sanford, NC 27332 • Phone: 919-774-5557 • Fax: 919-776-6785
-------
CHAIN OF CUSTODY RECORD
Project Name
US EPA
Project Location
RTF, NC
Sampling Location
ACB Outlet Duct
Project Number
RN99027 1.0002
Laboratory
Resolution Analytics
Laboratory P.O. #
Sample ID
Sample Matrix
Analysis Requested
IR-ACB-PM-OJL
ACB-PM-FB
Acetone Blank — "
Dry filter *""'"
Front Half Acetone Rinse
Dry filter ""
Front Half Acetone Rinse
Particulate
Particulate
Particulate
Particulate
Particulate
I/
Notes/Comments:
Field blank collected 2/9/1 1 . Emission sample collected 2/1 6/11.
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919)544-4535
Fax Number: (919)544-5690
-------
RESOLUTION ANALYTICS, INC.
Specialists in Air Emissions Analysis
Report Summary
Client: Arcadis
RFA#: RN990271.0002
Method: EPAM5
SAMPLE ID
Acetone Blank
1R-ACB-PM-02
ACB-PM-FB
In House Filter Blank
TOTAL FILTERABLE
PARTICIPATE
0.4 mg (in 220 mis)
59.6 mg
0.8 mg
0.5 mg
-------
Client: Arcadis
RFA#: RN990271.0002
RESOLUTION ANALYTICS, INC,
Specialists in Air Emissions Analysis Analyst: use
Analysis: EPA M5
Date Received: 2/22/11
Date Analyzed: 2/23/11
Analyst: JSC
Analysis: EPA M5
Analyte(s): Filterble Participate
Analytical Narrative
Sample Matrix & Components:
Dry Filters, Front/^ Acetone Rinses, Acetone Blank
Summary of Sample Prep:
The acetone rinses were transferred to pre-tared teflon "baggies" in a low humidity environment. The
acetone rinses were evaporated overnight then desiccated for 24 hours, after which time they were weighed
daily every six hours until consecutive weights agreed within ±0.5 mg. The filters were baked 2 to 3 hours
at 105° C, desiccated for 2 hours and weighed.
All weights were recorded to the nearest 0.1 mg and include filterable paniculate catch only. The total
catch reported for each run is a sum of the filter and rinse catches. The acetone blank catch has been
subtracted from sample rinse catches in proportion with their respective volumes.
Summary of Instrumentation:
Denver model Pinnacle Series analytical balance
Analytical Detection Limit(s): 0.1 mg
Miscellaneous Comments Regarding Sample Analysis: (Note unusual catch weights, interferences, odd sample
behavior, and steps taken to confirm unusual results. Also note any deviations from standard analytical
procedures, together with justification and possible affect on results. Specify samples when applicable.)
No modifications to EPA Method 5 analytical procedure were made. See data sheets for individual sample
descriptions.
-------
PARTICULATE SAMPLING LABORATORY RESULTS
Client: Arcadis
Method: EPA M5
RFA#: RN990271.0002
Run Number
1R-ACB-PM-02
ACB-PM-FB
In House Filter Blank
Filter Container #
Date
2/23/11
Init Date
JSC 0.4066 2/23/11
Baggie Tare Wt., g. #N/A
Filter Tare Wt., g. 0.3526
FILTER SAMPLE WT., g. 0.0540
Date
0.3549 2/23/11
#N/A
0.3545
0.0004
0.3542
#N/A
0.3537
0.0005
Front 1A Rinse Container #
2572
2560
Date
Init
Date
Date
2/24/11 JSC F
2/23/11 JSC F
Tare Wt., g. ( 60 ml)
RINSE SAMPLE WT., g.
Filter Catch, mg.
Rinse Catch, mg.
Rinse Blank Residue, mg.
Net Rinse Catch, mg.
FILTERABLE PARTICULATE, mg.
3.5353 2/14/11
3.5353 2/23/11 F
3.5296 ( 10 ml)
0.0057
54.0
5.7
0.1
5.6
59.6
3.5442
3.5441
3.5437 (
0.0004
0.4
0.4
0.0
0.4
0.8
m
ml) #N/A
#N/A
0.5
#N/A
0.0
#N/A
0.5
Legend: F = Final Weight
Notes & Comments:
-------
REAGENT BLANK LABORATORY RESULTS
Client: Arcadis
Method: EPA M5
RFA#: RN990271.0002
Run Number
Acetone Blank
Sample ID/Container #
Date
2614
Init
Tare Wt, g.
SAMPLE WT., g.
Blank Beaker #
Final wt, mg.
Tare wt., mg.
Residue, mg.
Volume, ml.
Density, mg/ml
Cone., mg/mg
Upper Limit, mg
I Legend: F
2/24/11 JSC
2/23/11 JSC F
( 220 ml)
2614
3.5328
3.5324
0.4
220
785.0
2.32E-06 <-
1.00E-05
= Final Weight \
3.5329
3.5328
3.5324
0.0004
Notes & Comments:
-------
o
CO
u
c
0)
DO
0)
•*-»
JO
3
O
1
CO
CL
U
C 0
0) >.
s s
0
tsQ
0
^
u,
o
"2r
3
o
"S-
Or
R
-------
ARCADIS-US: Durham
4915 Prospectus Drive, Suite F
Durham, NC 27713
ACB Outlet Duct
Client RN990271.0002
PO#RB68316
Analytical Report
(0111-13)
EPA SW-846 Method 8270C
Polyaromatic Hydrocarbons - SCAN Mode
Enthalpy Analytical, Inc.
j^£$'* | Phone: (919)850-4392 / Fax: (919)850-9012 / www.enthalpy.com
,/';"* ' 2202 Ellis Road Durham, NC 27703 - 5518
-------
I certify that to the best of my knowledge all analytical data presented in this report:
• Have been checked for completeness
• Are accurate, error-free, and legible
• Have been conducted in accordance with approved protocol, and that all deviations and
analytical problems are summarized in the appropriate narrative(s)
This analytical report was prepared in Portable Document Format (.PDF) and contains 112 pages.
QA Review Performed by: Michael Steven Schapira
Report Issued: 2/4/11
EA# 0111-13 Page 2 of 112
-------
Results
EA# 0111-13 Page 3 of 112
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run
Client*
Job*
P0#
Report Date
RN990271.0002
0111-13
RB68316
1/28/2011
Sample ID: 0111-13 Run 1
Data File: W1100050.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
6.52
7.20
7.30
8.12
NA
8.80
9.79
9.84
11.0
11.2
12.5
12.6
14.1
14.1
14.6
14.6
NA
16.8
NA
17.3
5.29
6.50
8.25
9.77
12.6
14.7
4.17
4.89
5.79
7.54
8.78
9.05
11.2
11.3
Area
3,869,796
201 ,854
151,061
595,949
0
149,347
756,841
74,643
412,863
367,230
61,611
57,553
62,551
24,878
26,025
36,754
0
26,218
0
28,060
568,852
2,065,421
1,212,574
2,206,499
2,022,092
1,621,593
257,460
287,479
331 ,339
727,430
607,572
88,844
892,507
749,952
Cone.
(ug/mL)
77.8
6.34
4.90
12.2
0.934
4.28
15.2
1.50
7.97
6.60
1.26
1.25
1.54
0.628
0.720
0.986
0.340
0.711
0.425
0.933
40.0
40.0
40.0
40.0
40.0
40.0
16.6
15.9
18.7
19.4
18.2
17.6
19.4
20.5
Catch
Weight
(ug)
389
31.7
24.5
61.1
4.67
21.4
76.0
7.50
39.8
33.0
6.31
6.26
7.71
3.14
3.60
4.93
1.70
3.55
2.13
4.66
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
83.0
79.7
93.6
97.0
91.2
87.9
97.0
102
Flags
J
ND
J
J
J
J
J
J
J
J
ND
J
ND
J
Spk
Amt
100
100
100
100
100
100
100
100
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.29
6.50
8.26
9.77
12.57
14.75
Sample
RT
5.29
6.50
8.25
9.77
12.56
14.75
Concal
IS Area
563,777
1,972,475
1,079,691
1,925,531
1,937,140
1,582,057
Sample
IS Area
568,852
2,065,421
1,212,574
2,206,499
2,022,092
1,621,593
Flag
Pass
Pass
Pass
Pass
Pass
Pass
EA# 011J-J3 hPage.4 of 112
0111-13 pah summaries
1/28/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run
Client*
Job*
P0#
Report Date
RN990271.0002
0111-13
RB68316
1/28/2011
Sample ID: 0111-13 Run 1 LD
Data File: W1100051.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
6.52
7.20
7.30
8.12
NA
8.81
9.79
9.85
11.0
11.2
12.5
12.6
14.1
14.1
14.6
14.6
NA
16.8
NA
17.3
5.29
6.50
8.25
9.77
12.6
14.8
4.17
4.89
5.79
7.54
8.78
9.05
11.2
11.3
Area
4,009,940
199,158
155,319
612,696
0
152,878
795,360
78,485
437,252
400,273
73,616
69,071
87,991
24,140
33,693
47,912
0
32,792
0
33,051
583,750
2,045,145
1,183,673
2,217,759
2,296,315
1,959,935
280,212
315,905
341,189
745,275
622,954
101,331
989,539
846,772
Cone.
(ug/mL)
81.4
6.31
5.09
12.9
0.934
4.48
15.9
1.57
8.39
7.15
1.33
1.32
1.80
0.504
0.772
1.06
0.340
0.736
0.425
0.909
40.0
40.0
40.0
40.0
40.0
40.0
17.6
17.1
18.8
20.4
19.2
20.0
21.4
23.0
Catch
Weight
(ug)
407
31.6
25.5
64.3
4.67
22.4
79.4
7.85
42.0
35.8
6.64
6.61
8.98
2.52
3.86
5.32
1.70
3.68
2.13
4.55
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
88.0
85.3
93.9
102
95.8
99.8
107
115
Flags
ND
J
J
J
J
J
J
J
J
ND
J
ND
J
Spk
Amt
100
100
100
100
100
100
100
100
% Diff
4.6
0.4
3.8
5.3
0.0
4.9
4.6
4.6
5.4
8.4
5.2
5.7
16.4
19.7
7.1
7.9
0.0
3.5
0.0
2.5
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.29
6.50
8.26
9.77
12.57
14.75
Sample
RT
5.29
6.50
8.25
9.77
12.56
14.75
Concal
IS Area
563,777
1,972,475
1,079,691
1,925,531
1,937,140
1,582,057
Sample
IS Area
583,750
2,045,145
1,183,673
2,217,759
2,296,315
1,959,935
Flag
Pass
Pass
Pass
Pass
Pass
Pass
EA# 011J-J3 hPage.5 of 112
0111-13 pah summaries
1/28/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run
Client*
Job*
P0#
Report Date
RN990271.0002
0111-13
RB68316
1/28/2011
Sample ID: 0111-13 MB
Data File: W1100049.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5.29
6.50
8.25
9.77
12.6
14.8
4.16
4.88
5.79
7.54
8.78
9.05
11.2
11.3
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
522,090
1,845,188
1,083,822
1,974,624
1,995,220
1,733,409
218,019
261 ,447
226,918
539,767
514,511
80,797
828,814
634,438
Cone.
(ug/mL)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
40.0
40.0
40.0
40.0
40.0
40.0
15.3
15.8
14.0
16.1
17.3
17.9
20.1
19.4
Catch
Weight
(ug)
3.33
3.09
3.07
3.37
4.67
3.92
3.86
3.80
4.60
4.69
2.75
2.78
1.50
1.78
1.70
1.70
1.70
1.50
2.13
1.76
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
76.6
79.0
69.8
80.5
86.4
89.4
101
96.8
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Spk
Amt
100
100
100
100
100
100
100
100
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.29
6.50
8.26
9.77
12.57
14.75
Sample
RT
5.29
6.50
8.25
9.77
12.56
14.75
Concal
IS Area
563,777
1,972,475
1,079,691
1,925,531
1,937,140
1,582,057
Sample
IS Area
522,090
1,845,188
1,083,822
1,974,624
1,995,220
1,733,409
Flag
Pass
Pass
Pass
Pass
Pass
Pass
EA# 011J-J3 hPage.6 of 112
0111-13 pah summaries
1/28/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run
Client*
Job*
P0#
Report Date
RN990271.0002
0111-13
RB68316
1/28/2011
Sample ID: CH2CI2 System Blank (Scan)
Data File: W1100045.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5.29
6.50
8.26
9.77
12.6
14.7
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
554,892
1,981,163
1,122,172
1,929,188
1,719,405
1,512,053
Cone.
(ug/mL)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
40.0
40.0
40.0
40.0
40.0
40.0
Catch
Weight
(ug)
3.33
3.09
3.07
3.37
4.67
3.92
3.86
3.80
4.60
4.69
2.75
2.78
1.50
1.78
1.70
1.70
1.70
1.50
2.13
1.76
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Spk
Amt
Flags
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.29
6.50
8.26
9.77
12.57
14.75
Sample
RT
5.29
6.50
8.26
9.77
12.56
14.75
Concal
IS Area
563,777
1,972,475
1,079,691
1,925,531
1,937,140
1,582,057
Sample
IS Area
554,892
1,981,163
1,122,172
1,929,188
1,719,405
1,512,053
Flag
Pass
Pass
Pass
Pass
Pass
Pass
EA# 011J-J3 hPage.7 of 112
0111-13 pah summaries
1/28/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method 8270C
# Samples 1 Run
Sample ID: 0111-13 LCS
Data File: W1100043.D
Tank/Misc ID:
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Client*
Job*
P0#
Report Date
RN990271
0111-13
RB68316
1/28/2011
0002
DF:
Aliquot Factor:
Extraction Vol (mL):
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
6.52
7.20
7.30
8.12
8.29
8.81
9.79
9.85
11.0
11.3
12.6
12.6
14.1
14.1
14.6
14.7
14.8
16.8
16.8
17.3
5.29
6.50
8.26
9.77
12.6
14.8
4.16
4.88
5.79
7.54
8.78
9.05
11.2
11.3
Area
840,530
543,266
523,921
828,230
518,942
615,875
870,089
851,163
913,598
977,418
809,408
745,983
689,771
698,187
670,300
634,625
643,038
594,964
498,597
478,483
553,789
1,980,939
1,132,651
1,983,150
1,750,154
1,422,438
254,174
307,408
293,850
602,034
571 ,950
86,854
781,120
587,552
Cone
(ug/mL)
17.6
17.8
17.7
18.2
17.8
18.9
19.4
19.0
19.6
19.5
19.2
18.7
19.4
20.1
21.2
19.4
18.8
18.4
18.3
18.1
40.0
40.0
40.0
40.0
40.0
40.0
16.8
17.5
17.0
17.2
18.4
19.1
18.9
17.9
ConCal 1
Catch
Weight
(ug)
88.1
88.9
88.6
90.9
89.1
94.4
97.2
95.2
98.1
97.7
95.8
93.7
97.0
100
106
97.0
94.2
92.0
91.4
90.7
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
84.2
87.5
85.2
86.0
91.9
95.6
94.5
89.3
1.00
1.00
5.00
Flags
Spk
Amt
100
100
100
100
100
100
100
100
-------
Narrative Summary
EA# 0111-13 Page 9 of 112
-------
Enthalpy Analytical Narrative Summary
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA SW-846 Method 8270C
1 Run
Client #
Job#
PO#
Report Date
RN99027 1.0002
0111-13
RB68316
January 3 1,20 11
Custody Heather Tarjeft received the sample on 1/24/11 after being relinquished
by ARCADIS-US: Durham. The sample was received at 7.4°C and in
good condition. Prior to, during, and after analysis, the sample was kept
under lock with access only to authorized personnel by Enthalpy
Analytical, Inc.
Analysis The sample was analyzed for SW-846 Method 8270C Polyaromatic
Hydrocarbons (PAHs) - SCAN Mode using the analytical procedures in
EPA SW-846 Method 827OC, Semivolatile Organic Compounds by Gas
Chromatography/Mass Spectrometry (GC/MS).
Prior to sample collection, the XAD-2 traps were spiked with a BN
Surrogate Mix (100 jig of nitrobenzene-ds, 2-fluorobiphenyl, and
terphenyl-di/t).
The sample train consisted of five fractions: Filter, XAD Trap, FH
Rinse, BH Rinse, and Condensate. All rinse fractions were
methanol/methylene chloride.
The sample was spiked with the three acid extraction surrogates (100 jig
of 2-fluorophenol, phenol-d5, and 2, 4, 6-tribromophenol) and PAH
extraction surrogates (fluorene-dio and pyrene-dio); 50% spiked into the
XAD/Filter and 50% spiked into the condensate.
The filter and XAD-2 fractions were Soxhlet extracted for 18 hours. The
rinse and condensate fractions were combined and Separately funnel
extracted using methylene chloride.
The Condensate/Rinse and Filter/XAD-2 extracts were combined and
concentrated to a 5 mL final volume.
An LCS and method blank were prepared and analyzed with these
samples. All QC samples were concentrated to a 5 mL final volume.
A 100-jiL aliquot of each client or QC sample extract was spiked with
SVGA internal standard spiking solution, before analyses.
•H'
EA# 0111-13 Page 10 of 112
-------
Enthalpy Analytical Narrative Summary
(continued)
Analysis
(continued)
Calibration
Chromatographic
Conditions
QC Notes
Reporting Notes
The Agilent Technologies Model 6890N, Gas Chromatograph ("Wiley"
S/N CN10244010) was equipped with a 5973 Mass Selective Detector
and a Restek rxi-5Sil MS, 30 m x 0.25 mm x 0.5 |im (SN 982029)
capillary column for these analyses.
The RSD of all target compounds in the initial calibration were within
the 20% acceptance limit.
All continuing calibration criteria were met in the analysis of these
samples.
The calibration curve is included in the Calibration Curve
Chromatograms section of this report.
A copy of the acquisition method RXI-2A-EA.M is included in the
Calibration Curve Chromatograms section of this report.
All internal standard criteria were met in the analyses of this sample.
The sample was analyzed in duplicate and met all duplicate criteria.
The analysis of the LCS exhibited recovery values of 80-120% for all
compounds.
All sample preparation and analytical holding times specified in the
method were met.
The results presented in this report are representative of the samples as
provided to the laboratory.
EA# 0111-13 Page 11 of 112
-------
General Reporting Notes
The following are general reporting notes that are applicable to all Enthalpy Analytical, Inc. data
reports, unless specifically noted otherwise.
• The acronym MDL represents the Minimum Detection Limit. Below this value the laboratory cannot
determine the presence of the analyte of interest reliably.
• The acronym LOQ represents the Limit of Quantification. Below this value the laboratory cannot
quantitate the analyte of interest within the criteria of the method.
• The acronym ND following a value indicates a non-detect or analytical result below the MDL.
• The letter / following a value indicates an analytical result between the MDL and the LOQ. A J flag
indicates that the laboratory can positively identify the analyte of interest as present, but the value
should be considered an estimate.
• The letter E following a value indicates an analytical result exceeding 100% of the highest calibration
point. The associated value should be considered as an estimate.
• The acronym DF represents Dilution Factor. This number represents dilution of the sample during the
preparation and/or analysis process. The analytical result taken from a laboratory instrument is
multiplied by the DF to determine the final undiluted sample results.
• The addition of MS to the Sample ID represents a Matrix Spike. An aliquot of an actual sample is
spiked with a known amount of analyte so that a percent recovery value can be determined. This shows
what effect the sample matrix may have on the target analyte, i.e. whether or not anything in the sample
matrix interferes with the analysis of the analyte(s).
• The addition of MSD to the Sample ID represents a Matrix Spike Duplicate. Prepared in the same
manner as an MS, the use of duplicate matrix spikes allows further confirmation of laboratory quality
by showing the consistency of results gained by performing the same steps multiple times.
• The addition of LD to the Sample ID represents a Laboratory Duplicate. The analyst prepares an
additional aliquot of sample for testing and the results of the duplicate analysis are compared to the
initial result. The result should have a difference value of within 10% of the initial result (if the results
of the original analysis are greater than the LOQ).
• The addition of AD to the Sample ID represents an Alternate Dilution. The analyst prepares an
additional aliquot at a different dilution factor (usually double the initial factor). This analysis helps
confirm that no additional compound is present and coeluting or sharing absorbance with the analyte of
interest, as they would have a different response/absorbance than the analyte of interest.
• The Sample ID LCS represents a Laboratory Control Sample. Clean matrix, similar to the client sample
matrix, prepared and analyzed by the laboratory using the same reagents, spiking standards and
procedures used for the client samples. The LCS is used to assess the control of the laboratory's
analytical system. Whenever spikes are prepared for our client projects, two extra spikes are prepared.
The extras (randomly chosen) are labeled with the associated project number and kept in-house at the
appropriate temperature conditions. When the project samples are received for analysis, the LCSs are
analyzed to confirm that the analyte could be recovered from the media, separate from the samples
which were used on the project and which may have been affected by source matrix, sample collection
and/or sample transport.
EA# 0111-13 Page 12 of 112
-------
General Reporting Notes
(continued)
Significant Figures: Where the reported value is much greater than unity (1.00) in the units expressed,
the number is rounded to a whole number of units, rather than to 3 significant figures. For example, a
value of 10,456.45 ug catch is rounded to 10,456 ug. There are five significant digits displayed, but no
confidence should be placed on more than two significant digits.
Manual Integration: The data systems used for processing will flag manually integrated peaks with
an "M". There are several reasons a peak may be manually integrated. These reasons will be identified
by the following two letter designations. The peak was not integrated by the software "NI", the peak
was integrated incorrectly by the software "II" or the wrong peak was integrated by the software
"WP". These codes will accompany the analyst's manual integration stamp placed next to the
compound name.
_ ""»"i^'rfs
-------
Sample Custody
B
Sf fe/
EA# 0111-13 Page 14 of 112
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
US EPA
RTP, NC
ACB Outlet Duct
RN990271
Enthalpy
.0002
Sample ID
Sample Matrix
Analysis Requested
1R-ACB-M0010-01
Dry filter
Front Half MeOH/MeCl2 Rinse
Impinger DI H2O
Back Half MeOH/MeC!2 Rinse
XAD trap
BLANKS
MeOH
MeCl2
DIH2O
SVOC
SVOC
SVOC
SVOC
SVOC
Archive
Archive
Archive
Notes/Comments:
= .
Relinquished By:
Date/Time:
Received By:
Date/Time:
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919) 544-4535
Fax Number: (919)544-5690
EA# 0111-13 Page 15 of 112
-------
Sample
Chromatograms
(Scan)
EA# 0111-13 Page 16 of 112
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100050.D
28 Jan 2011 5:25 pm
tdd
0111-13 Run 1
10 Sample Multiplier: 1
Quant Time Jan 28 18:27:30 2011
Quant Method
Quant Title
QLast Update
Response via
Abundance
9500000
9000000
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
0
M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Jan 04 17:58:34 2011
Initial Calibration
TIC: W1100050.D\data.ms
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Jan 28 18:34:29 2011
3.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100050.D
28 Jan 2011 5:25 pm
tdd
0111-13 Run 1
10 Sample Multiplier: 1
Quant Time Jan 28 18:27:30 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.290
. 502
.255
. 767
. 561
.746
152
136
164
188
240
264
568852
2065421
1212574
2206499
2022092
1621593
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
-0 .
-0.
.00
. 00
. 00
. 00
. 01
.02
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.168 112 257460
5) Phenol-d5 (surr) 4.894 99 287479
19) Nitrobenzene-d5 (surr) 5.787 82 331339
39) 2-Fluorobiphenyl (surr) 7.539 172 727430
57) Fluorene-dlO (surr) 8.778 176 607572
65) 2,4,6-Tribromophenol (... 9.051 330 88844
75) Pyrene-dlO (surr) 11.225 212 892507
77) Terphenyl-dl4 (surr) 11.343 244 749952
Target Compounds
16.60 ug/mL
15.94 ug/mL
18.71 ug/mL
19.40 ug/mL
18.25 ug/mL
17.59 ug/mL
19.41 ug/mL
20.49 ug/mL
-0.01
0 . 00
0 . 00
-0 . 01
0 . 00
-0.01
0.00
0.01
Qvalue
Pyridine
Phenol (CCC)
Aniline
bis(2-Chloroethyl)ether
2-Chlorophenol
1,3-Dichlorobenzene
1,4-Dichlorobenzene (CCC)
Benzyl alcohol
1,2-Dichlorobenzene
2-Methylphenol
bis(2-Chloroisopropyl)...
3/4-Methylphenol
N-Nitrso-di-n-propylam.
Hexachloroethane
Nitrobenzene
Isophorone
2 , 4-Dimethy^UpfTenol
2-Nitropte£nol (CCC)
Benzole acid
(2-Chloroethoxy)met...
"2,4-Dichlorophenol (CCC)
u . u u u
3 .233
4 . 905
0.000
0.000
0.000
0.000
0 . 000
0 . 000
0 . 000
5 .498
O.OC
14
'0.000
0.000
0 . 000
0 . 000
0 . 000
6 . 112
6.171
0.000
0.000
N.D.
17 .37
64 . 74
N.D
N.D
ug/mL#
ug/mL#_
. d
91
94
107
94
,4-Tliuhll
Naphthalene
128 3869796
77 . 80
4-Chlun
TJovan>-i1 ,
u . u u u
n nrm
ug/mL#
—d
62
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D. d
2-Methylnaphthalene
1-Methylnaphthalene
7.197
7.298
n n n n
142
142
201854
151061
6.34
4.90
•NT •n
ug/mL
ug/mL
100
100
0.000
0 . 000
0 . 000
0 . 000
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
, 2 -Diiiiti-obe
0111-13 Page 18 of
W101810X-8270.M Fri Jan 28 18:34:28 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100050.D
28 Jan 2011 5:25 pm
tdd
0111-13 Run 1
10 Sample Multiplier: 1
Quant Time Jan 28 18:27:30 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
Response
595949
Cone Units Dev(Min)
Acenaphthylene
3 Mitroaniline
0.68 ug/mL#
Acenaphthene
8.287 154
0.000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
8.458 16
5.88 ug/mL#
N.D.
N.D.
N.D. d
M n
Tetrachlorophenol
4.28 ug/mL
N.D. d
Fluorene
i-Niti-oaiiiliiie
8.805 166
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D.
N.D.
N.D.
N.D.
Phenanthrene
Anthracene
15.19 ug/mL
1.50 ug/mL
n T a -iirT/mT.-H-
99
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
10.258 ±49-
30231
0.55 ug/mL
11.006 202
412863
r> r> r> r>
7.97 ug/mL
—^
97
95
76)
79T
80)
81)
82)
Pyrene
11.246 202
367230
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipat^
bis(2 ~
6.60 ug/mL#
L _L . / ^ _L ±^± iJ
0 . 000
149
6164
63373
Q_
0.31 ug/mL#
2.07 ug/mL#
84) Benzo(a)anthracene
85) Chrysene
12.545
12.593
228
228
61611
57553
i) DJ
jLyluhLlidldLb
0.000
14.088
14.115
14.553
14.644
14.794
16.760
0.000
17.342
1.26 ug/mL#
1.25 ug/mL#
N.D. d
100
99
1
64
. y j-jjiiLiid-LciLt: rc^
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
252
252
252
252
252
276
276
62551m
24878m
26025
36754
7208
26218
0
28060
1.54 ug/mL
0.63 ug/mL
0.72 ug/mL
0.99 ug/mL
N.D.
0.71 ug/mL
N.D. d
0.93 ug/mL
97
92
100
97
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 19 of 112
W101810X-8270.M Fri Jan 28 18:34:28 2011
Page: 2
-------
Abundance
Ref 50
Scan 849 (8.068 min): W1000111.D (-841) (-)
lie
51 75 102
149 175 202 240 268
Abundance
Raw
50
50 100 150 200 250 300
Scan 663 (6.524 min): W1100050.D\data.ms
128
39
63
102
150 177 207
m/z~>
Abundance
341
350
#29
Naphthalene
Concen: 77.80 ug/mL
RT: 6.524 min Scan# 663
Delta R.T. 0.000 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:128 Resp: 3869796
Ion Ratio Lower Upper
128 100
129 11.7 17.0 25.6#
127 13.4 35.1 52.7#
Abundance
Sub
50
50 100 150 200 250 300 350
Scan 663 (6.524 min): W1100050.D\data.ms (-624) (-)
lie
51 74
102
149 177 207
3000000
2000000
1000000
0
6.824
50
100
150
200
250
300
350 Time-> 6.45 6.50 6.55 6.60 6.65
Abundance
Ref 50
Scan 966 (8.755 min): W1000111.D (-959) (-)
1
115
39
63
89
Abundance
Raw 50
m/z~>
Abundance
Sub
50
166193 231
281 327
387
50 100 150 200 250 300 350
Scan 789 (7.197 min): W1100050.D\data.ms
400
115
63 89
#33
2-Methylnaphthalene
Concen: 6.34 ug/mL
RT: 7.197 min Scan# 789
Delta R.T. 0.004 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:142 Resp: 201854
Ion Ratio Lower Upper
142 100
141 86.6 69.7 104.5
Abundance
200000
169 207
281
50 100 150 200 250 300 350 400
Scan 789 (7.197 min): W1100050.D\data.ms (-749) (-)
142
115
63 89
150000
100000
50000
169
281
50
100 150 200 250 300 350 400 Time->
7.15
7.197
7.20
W1100050.D W101810X-8270.M
EA# 0111-13 Page 20 of 112
Fri Jan 28 19:11:11 2011
Page
-------
Abundance Scan 984 (8.861 min): W1000111.D (-978) (-)
Ref 50
o
1'
115
39 63 89
i J, il
\2
162 195 221 271 295 327
m/z~> 50 100 150 200 250 300
Abundance Scan 808 (7.298 min): W1 1 00050. D\data.ms
Raw 50
o
1.
115
39 63 89
J.I.I.. I.,.,...!!.,,.!,!,!!, I.I I I,
Ll
\2
165 193 221 269
#34
1 -Methylnaphthalene
Concen: 4.90 ug/mL
RT: 7.298 min Scan# 8
Delta R.T. 0.005 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25
Tgt Ion:142 Resp: 15106
Ion Ratio Lower Upper
142 100
141 90.8 72.5 108.7
Abundance
150000
m/z-> 50 100 150 200 250 300
Abundance Scan 808 (7.298 min): W1 100050. D\data.ms (-768) (-) 100000
Sub „
50
o
1.
115
39 63 89
.1... ,..,.,1. J...I L ... 1 .....
., Ll
\2
50000
163 193 221 269 o
7i98
I
\\
I \
I V
m/z-> 50 100 150 200 250 300 [Time-> 7.25 7.30 7.35
Abundance Scan 1 125 (9.689 min): W1 00011 1.D (-11 18) (-) #47
Ref 50
o
1J
76
50 j J.1P11?6 I
m/z~> 50 100 15
'2 Acenaphthylene
177202 253 402
Concen: 12.22 ug/mL
RT: 8.116 min Scan# 9
Delta R.T. -0 . 012 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25
0 ' ' 200 ' ' 250 ' ' 300 ' ' 350 ' ' 400 T9fc Ion: 152 Resp: 59594
Abundance Scan 961 (8.1 16 min): W11 00050. D\data.ms
Raw 50
o
1
76 ._„
50 t j 101 126
m/z~> 50 100 15
2
178207 268
Ion Ratio Lower Upper
152 100
151 22.8 15.0 22.4
153 13.3 10.2 15.2
Abundance
500000
0 200 250 300 350 400 400000
Abundance Scan 961 (8.116 min): W1 100050. D\data.ms (-924) (-)
Sub
50
1J
51,? 101 126
1 " '""' '"'' 1""'" |1"1'
m/z~> 50 100 15
2
176 208 268
300000
200000
100000
0
8.116
A
If Nil
0 200 250 300 350 400 [Time~> 8.05 8.10 8.15 8.20
W1100050.D W101810X-8270.M
EA# 0111-13 Page 21 of 112
Fri Jan 28 19:11:11 2011
Page 7
-------
Abundance Scan 1243 (10.383 min): W1 00011 1 .D (-1233) (-) #59
Ref 50
o
1f
,51,l,l8211l31f I
i6 Fluorene
Concen: 4.28 ug/mL
RT: 8.805 min
Scan# 1
Delta R.T. -0.005 min
Lab File: W1100050.D
Acq: 28 Jan 2011
198 232 264 355 429
m/z~> 50 100 150 200 250 300 350 400
Abundance Scan 1090 (8.805 min): W11 00050. D\data.ms
Raw 50
0
1
-30 82 1 39
39 1 1 V i
i,.,. i ,i LI,,., iAi,
i6
1 92 222 282
I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I
m/z-> 50 100 150 200 250 300 350 400
Abundance Scan 1090 (8.805 min): W11 00050. D\data.ms (-1052) (-)
Sub
50
o
1
82 -1-30
50n 111 ^
i6
Tgt Ion: 166 Resp
5 :25
: 14934
Ion Ratio Lower upper
166 100
165 94.2 75.4 113.0
167 16 . 1 10.9 16 .3
Abundance
100000
50000
1 93 237 282 o
I 05
f
^/""X^ J/^/
vV ^
m/z-> 50 100 150 200 250 300 350 400 Jime-> 8.70 8.75 8.80 8.85
pm
Abundance
Ref 50
0
m/z-->
Abundance
Raw 50
0
m/z-->
Abundance
Sub 50
0
m/z-->
Scan 141 2 (11. 376 min)
r
76 152
50 H j j98 126 j
: W1 0001 11.D (-1400) (-) #69
'8 Phenanthrene
200 227 267 303 327
50 100 150 200 250 300
Scan 1275 (9.793 min): W11 00050. D\data.ms
178
76 152
50 j j98 126 j
5
0 1 00 1 J
198218 251 281 330
50 200 250 300
Scan 1 275 (9.793 min): W1
r
39
.?> 126 1f „
50 1 00 1 J
1 00050. D\data.ms (-1237) (-)
'8
199219 254 281
Concen: 15.19 ug/mL
RT: 9.793 min Scan# 1275
Delta R.T. -0.005 min
Lab File: W1100050.D
Acq: 28 Jan 2011
Tgt Ion: 178 Resp:
Ion Ratio Lower
178 100
179 16.2 12.5
176 18.8 15.0
Abundance
600000
400000
200000
0
50 200 250 300 [Time->
9.|
J
5:25 pm
756841
Upper
18.7
22.4
33
i^\
9.70 9.75 9.80 9.85
W1100050.D W101810X-8270.M
EA# 0111-13 Page 22 of 112
Fri Jan 28 19:11:12 2011
Page 9
-------
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O O rH PI
O (Z| O rH rH Cfl
*£> -H • rH O 0)
• £ o ;s CM pe;
*£>
VD Hi (M
•sh • (ti O
CN EH •• ID CN -
in LD ^H ^ ^^^__J
£ ^ n "ai=^ O O O ^*\
•• rH pel rH CO Hi
(ti O CN Tf
_
o
°?
- "*~
-
m
CN
~ ^
I 0
CN
- T~
- ^~
-
-
(D £ rH -HCN O PS rH
o >i o EH CD (ti u tn
* ft u PC; Q j CN
> CD
co
^ CN
—
E CN
CD ^
05 S-
CN T-
—
oo oo
00 CN
CD t-
( — )
(— *— ' ^
co """ -
CO i^
CD
co
-
-
0
o
-
0
-m
co
o
o
co
0
CN
-
-
0
o
CN
O
— m
^
-
-
o
°.
-
-
rS
-
8 §
cz
co
•0 M-l A
§ ^ rij
O PH ^^-
ra ° ° ° tt* A
aCNmOO-O § § § 0) I
Oooooc co CN •«— CQcb
H CN] CN] ^2 n E
< i-
w
E
JO
"co
^^ ^ —
^ s
0
m
o
o
o T;'"
^ CN
> CD
> m
CN
^~ CD
•- CN
E CN
CD ^
^^ C^
c^ i
^~~ r^ ~
r^ LO
"^" CO
LO ¥
r^ ^~
CO
O ^ -
CO -*,
co
"*
-
-
0
o
-
0
-m
co
o
o
co
0
— m
CN
CO
0? ^~
0 T—
m ^_
^L O
w it
^^OsJ
1 ^
Q
0 CN
§ s
8 g
•<- CN
^~
^>
0 • • CN
:«|- 5
o
— m
^
-
-
o
°.
-
-
lo
CD T-
CN
•^ 00
T- CN
' ^ T —
t 0
^" — ~~
m ^
T — ^>
C f^1
co
o
_ h" co
-
-
0
o
-
0
-m
co
o
o
co
0
-m
CN
-
-
o
o
CN
O
-m
^
-
-
o
°.
-
-
lo
-
g 0 0 g 0 0
d CZ
co co
•0 £ A -0 ,Q A
O Pn -t!
< E < E
^ r^
E co -t!
< E
-------
Abundance
Ref 50
0
Abundance
Raw 50
0
m/z-->
Abundance
Sub 50
0
m/z-->
Scan 2011 (1 4.895 mir
2i.
114
50 86 I 163 200
): W1 000 111. D (-2000) (-)
3
258 297326355 429
50 100 150 200 250 300 350 400 450
Scan 1790 (12.545 min): W1 100050.D\data.ms
240
120
52, ,,92,1 1561 8421 2^
50 100 150 200
Scan 1 790 (12.545 min): \A
2
120
54, ..92 j 148182212,.
, 268 304 339 401 461
250 300 350 400 450
1 100050.D\data.ms (-1753) (-)
4o
h 268296325 401 461
50 100 150 200 250 300 350 400 450
#84
Benzo (a) anthracene
Concen: 1.26 ug/mL
RT: 12.545 min Scan# 1790
Delta R.T. -0 . Oil min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:228 Resp: 61611
Ion Ratio Lower Upper
228 100
229 21.8 14 . 7 22 . 1
226 139.1 21.0 31. 6#
Abundance
40000
30000
20000
10000
0
Time~>
\
i\
I / --
/ v\
J / *—
12.50 12.55
/
Abundance
Ref 50
0
m/z-->
Abundance
Raw 50
0
m/z-->
Abundance
Sub 50
0
m/z-->
Scan 2023 (14.966 mir
2;
113
39 75. ill 151 20,°
i I
50
i I
50
Scan
44
i i
50
100 150 200
Scan 1799 (12.593m
2;
i yIH : j |i : , ijjr M|
100 150 200
1 799 (12.593 min): \A
2;
114
85 j 14417220,0
1C
):W1000111.D(-2016)(-)
!8
282 342 446
250 300 350 400
n): W1100050.D\data.ms
8
J,
281 309 355 415
250 300 350 400
1 100050.D\data.ms (-1763) (-)
8
,258286 329356 415
#85
Chrysene
Concen: 1.25 ug/mL
RT: 12.593 min Scan# 1799
Delta R.T. -0.016 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:228 Resp: 57553
Ion Ratio Lower Upper
228 100
226 0.0 23.6 35. 4#
229 23.4 15.8 23.6
Abundance
40000
30000
20000
10000
0
)0 150 200 250 300 350 400 [Time->
i!
/ ^
12.55
\ 12.593
\ A
' \
y\
12.60 12.65
W1100050.D W101810X-8270.M
EA# 0111-13 Page 25 of 112
Fri Jan 28 19:11:14 2011
Page 14
-------
Abundance
Ref 50
Scan 2393 (17.140 min): W1000111 .D (-2376) (-)
2$2
126
38 74 | | 161 200,
295 342 415
549
m/z->
Abundance
Raw
m/z~>
50
50 100 150 200 250 300 350 400 450 500 550
Scan 2079 (14.088 min): W1100050.D\data.ms
2$2
207
73
125
39 i I 163
, ..ill L, I ii. I II Jl u
#88
Benzo(b)fluoranthene
Concen: 1.54 ug/mL m
RT: 14.088 min Scan# 2079
Delta R.T. -0.017 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:252 Resp: 62551
Ion Ratio Lower Upper
252 100
290325
401
I i 1-iVp
50 100 150 200 250 300 350 400 450 500 550
Abundance Scan 2079 (14.088 min): W1100050.D\data.ms (-2043) (-)
2$2
Sub
50
38
125
86 I I 163200
„, , ,j. I.. .II ii,. i ,. , ,, , .i [,, 1 .
290 339 401
50 100 150 200 250 300 350 400 450 500 550
125 20.1 7.7 11 . 5
Abundance
25000
20000
15000
10000
5000
0
Time~>
141)88
\
\
I
1
I
_^/^
\
\
\
\
\
\
\
\
14.05 14.10
Abundance Scan 2404 (17.204 min): W1 00011 1 .D (-2398) (-) #89
Ref 50
o
2!
1 26
50 83 | 157 200 , ,
>2 Benzo (k) f luoranthene
Concen:
0.63 ug/mL m
RT: 14.115 min Scan# 2
Delta R.T.
Lab File:
-0.038 min
W1100050.D
Acq: 28 Jan 2011 5:25
284 323355387 431 489
m/z-> 50 100 150 200 250 300 350 400 450 T9t Ion:252 Resp: 2487
Abundance Scan 2084 (14.115 min): W1 100050.D\data.ms Ion Ratio
Raw 50
o
2!
207
73 126
41 I I
2 252 100
283 355 429
253 80.4
125 50.6
Abundance
25000
m/z~> 50 100 150 200 250 300 350 400 450 20000
Abundance Scan 2084 (14.115 min): W1 100050. D\data.ms (-2052) (-)
Sub
50
Q
2!
126
35 74 Ti62 207,,
2
15000
10000
5000
295328 429 Q
\
1 \
\
1
/ V
^~ '""••--
Lower Upper
14.9 22.3
8.8 13.2
.115
\
\
\
\
\
\
- — x~ \ \
m/z-> 50 100 150 200 250 300 350 400 450 ' [lime-> 14JO 1415
W1100050.D W101810X-8270.M
EA# 0111-13 Page 26 of 112
Fri Jan 28 19:11:14 2011
Page 15
-------
Abundance Scan 2502 (1 7.780 min): W1 0001 1 1 .D (-2489) (-)
Ref 50
o
125
2'
43 74 J 174 222
#90
'2 Benzo ( e ) pyrene
Concen: 0.72 ug/mL
282 327 413446 489
RT: 14.553 min Scan# 2166
Delta R.T. -0.029 min
Lab File: W1100050.
Acq: 28 Jan 2011 5:
D
25
pm
m/z~> 50 100 150 200 250 300 350 400 450 T9t Ion:252 Resp: 26025
Abundance Scan 2166 (14.553 min): W1 100050.D\data.ms Ion Ratio Lower Upper
Raw 50
0
2C
73 125
43
/ L J,.,!.,!,,,,!,,,!,!,,,.!,16,5.,,!,!
I I
2J
7
Hi r.j
,2 252 100
250 29.6 23 . 0 34 .4
282 341 410
i I i i | i i i i | i i i i | i i i i | i i i i |
m/z-> 50 100 150 200 250 300 350 400 450
Abundance
Sub
50
o
Scan 2166 (14.553 min): W
2J
125
38 .73 , ,171209
253 24 .4 17 . 8 26.8
Abundance
20000
mnnn
1 100050.D\data.ms (-2134) (-)
>2
I 314 356 410
10000
5000
0
14.553
A
/ /\ \ \
If \\ //
A
A
1 \
m/z-> 50 100 150 200 250 300 350 400 450 Jime-> 14.45 14.50 14.55 14.60
Abundance Scan 2522 (1 7.897
Ref 50
o
1 26
min): W1 0001 11. D (-2511) (-) #91
2!
48 86 174211,,
m/z~> 50 100 150 200
'2 Benzo (a) pyrene
295327 387 430 503
(CCC)
Concen: 0.99 ug/mL
RT: 14.644 min Scan# 2183
Delta R.T. -0
.033 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
250 300 350 400 450 500 ^gt Ion: 252 Resp: 36754
Abundance Scan 2183 (14.644 min): W1 100050.D\data.ms Ion Ratio Lower Upper
Raw 50
0
2J
207
73 126
...,ii. ..ii,...ji L.,,,,.,,.iiiL.,... ,..,., ..i... i. . LPF?,, .LI .
i "i i 1 I I i [ r i i i i i
,,„
u^m
m/z~> 50 100 150 200
I |
2 252 100
283 325356 399
11
253 24.4 16.6 25.0
Abundance
20000
250 300 350 400 450 500 , 1i;mn
Abundance Scan 2183 (14.644 min): W1 100050.D\data.ms (-2150) (-)
Sub
50
o
126
40 74 _ 159 198 ,
m/z~> 50 100 150 200
2J
2
10000
5000
284 327 399 Q
14644
A
/
\
\
r
VV /
^^~=^J/
i
I
V_ y^"'
r
250 300 350 400 450 500 Time~> 14.60 14.65 14.70
W1100050.D W101810X-8270.M
EA# 0111-13 Page 27 of 112
Fri Jan 28 19:11:15 2011
Page 16
-------
Abundance Scan 3015 (20.794 min): W1 00011 1 .D (-2994) (-) #93
Ref 50
o
138
2'
39 92 , || 191 224
6 Indeno (1 , 2 , 3-cd) pyrene
Concen: 0.
311343 415446 503
m/z~> 50 100 150 200 250 300 350 400 450 500
Abundance Scan 2579 (1 6.
Raw 50
0
2
73
138
41 105, ill 174n
.ml, ,,ii.,....,i ..iMjiiiLiiiJiJiJiJiLJILIi...,!, L ill.
i i I i i i i I i i i i I i i i i
71 ug/mL
RT: 16.760 min Scan# 2579
Delta R.T.
0.066 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
Tgt Ion:276 Resp: 26218
760min):W1100050.D\data.ms lon *atio J-.ower upper
Ch7 276 100
276
,239, „
138 26.7
Abundance
341 401 475
i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i
10000
m/z-> 50 100 150 200 250 300 350 400 450 500 80OOJ
Abundance Scan 2579 (16.760 min): W1 100050.D\data.ms (-2552) (-)
Sub
50
0
138
2
42 96 174
HZ i i, , ,, i,
-r^T | i i i i | i i I i | i i i
2"
07
23?, ,
'6
6000
4000
2000
325357 416 475
i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i
0
21.4 32 .2
1 6./[60
/
1
\
f\\
I
iii
m/z-> 50 100 150 200 250 300 350 400 450 500 Time-> 16.70
Abundance Scan 31 57 (21 .628 min): W1 0001 1 1 .D (-31 37) (-)
Ref 50
o
138
2'
55 91 , 187222 ,
m/z~> 50 100 150 200
Abundance Scan 2688 (1 7.
Raw 50
o
2dr
73
138
3? ,! 117,21
m/z~> 50 100 150 200
Abundance
Sub
50
o
#95
i \
v^
1 1 1 1 1 1 1
16.80
'6 Benzo (g, h, i) perylene
Concen: 0.
93 ug/mL
RT: 17.342 min Scan# 2688
Delta R.T.
0.071 min
Lab File: W1100050.D
Acq: 28 Jan 2011 5:25 pm
323 368 416 489 549
250 300 350 400 450 500 550 T9fc I°n:276 Resp: 28060
342min):W1100050.D\data.ms Ion Ratio Lower Upper
7 276 100
276
i , id
138 25.5
277 25.8
Abundance
10000
341 387 429 475
1 ' 1 ' ] | i i i i | i i i i | i i i i | i i i i | i OUUU
250 300 350 400 450 500 550
Scan 2688 (17.342 min): M\
138
61 97,lj,172207,,, ,
m/z-> 50 100 150 200
1 100050.D\data.ms (-2662) (-)
'6
6000
4000
2000
329 401 461 o
r
//
^^J/
21.2 31.8
19.3 28.9
'ft42
I
A
\\
v^^^
250 300 350 400 450 500 550 Time~> 17.20 17.30 17.40
W1100050.D W101810X-8270.M
EA# 0111-13 Page 28 of 112
Fri Jan 28 19:11:15 2011
Page 17
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100051.D
28 Jan 2011 5:56 pm
tdd
0111-13 Run 1 LD
11 Sample Multiplier: 1
Quant Time Jan 28 18:33:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Jan 04 17:58:34 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
9500000
9000000
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
0
TIC: W1100051.D\data.ms
o
o
o
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Jan 28 18:34:11 2011
1 \ %°^ 1 1 -Q$
1 40°
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100051.D
28 Jan 2011 5:56 pm
tdd
0111-13 Run 1 LD
11 Sample Multiplier: 1
Quant Time Jan 28 18:33:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.290
. 503
.255
. 772
. 561
.751
152
136
164
188
240
264
583750
2045145
1183673
2217759
2296315
1959935
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
-0 .
-0.
.00
. 00
. 00
. 00
. 01
.01
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.168 112 280212
5) Phenol-d5 (surr) 4.895 99 315905
19) Nitrobenzene-d5 (surr) 5.787 82 341189
39) 2-Fluorobiphenyl (surr) 7.539 172 745275
57) Fluorene-dlO (surr) 8.778 176 622954
65) 2,4,6-Tribromophenol (... 9.051 330 101331
75) Pyrene-dlO (surr) 11.231 212 989539
77) Terphenyl-dl4 (surr) 11.343 244 846772
Target Compounds
17.61 ug/mL
17.07 ug/mL
18.78 ug/mL
20.36 ug/mL
19.17 ug/mL
19.96 ug/mL
21.41 ug/mL
23.01 ug/mL
-0.01
0 . 00
0 . 00
-0 . 01
0 . 00
-0.01
0.00
0.01
Qvalue
Pyridine 3.233
Phenol (CCC) 4.905
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 5.466
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 6.113
Benzpifcacid 6.171
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
N.D.
18 . 95
69 .14
N.D
N.D
ug/mL#
ug/mL#_
. d
92
107
95
,4-Tliuhll
Naphthalene
128 4009940
81 .41
4-Chlun
TJovan>-i1 ,
u . u u u
n nrm
ug/mL#
—d
61
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D. d
2-Methylnaphthalene
1-Methylnaphthalene
7.197
7.299
n n n n
142
142
199158
155319
6.31
5.09
ug/mL
ug/mL
100
99
0.000
0 . 000
0 . 000
0 . 000
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
, 2 -Diiiiti-obe
0111-13 Page30offl§-
W101810X-8270.M Fri Jan 28 18:34:08 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100051.D
28 Jan 2011 5:56 pm
tdd
0111-13 Run 1 LD
11 Sample Multiplier: 1
Quant Time Jan 28 18:33:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
Response
612696
Cone Units Dev(Min)
12.87 ug/mL# 94
Acenaphthylene
3 Mitroaniline
0.44 ug/mL#
N . D . d
Acenaphthene
8.287 154
0.000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
8.458 16
.12 ug/mL#
N.D.
N.D.
N.D. d
Tetrachlorophenol
4.48 ug/mL
N.D. d
Fluorene
i-Niti-oaiiiliiie
.811 166
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D.
N.D.
N.D.
N.D.
Phenanthrene
Anthracene
.793 178
.847 178
Q Q P £ 1
15.89 ug/mL
1.57 ug/mL
n AA -iirT/mT.-H-
100
7^-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
10.258 3r4£-
^^TTOCD
0.60 ug/mLft
11.006 202
437252
8.39 ug/mL#
N.D. d
97
Pyrene
11.247 202
400273
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipat^
bis(2 ~~
7.15 ug/mL#
L _L . / ^ _L ±^± iJ
0 . 000
149
0
70657
Q_
N.D. d
2.03 ug/mL#
84) Benzo(a)anthracene
85) Chrysene
12.545
12.593
228
228
73616m
69071
i) DJ
jLyluhLlidldLb
0.000
14.089
14.115
14.553
14.650
14.794
16.760
0.000
17.337
1.33 ug/mL
1.32 ug/mL#
N.D. d
99
. y j-jjiiLiid-LciLt: rc^
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
252
252
252
252
252
276
276
87991m
24140m
33693
47912
9078
32792
0
33051
1.80 ug/mL
0.50 ug/mL
0.77 ug/mL
1.06 ug/mL
N.D.
0.74 ug/mL
N.D. d
0.91 ug/mL
99
96
99
97
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 31 of 112
W101810X-8270.M Fri Jan 28 18:34:08 2011
Page: 2
-------
Abundance
Ref 50
Scan 849 (8.068 min): W1000111.D (-841) (-)
lie
51
.87,1
163 202 240
341
m/z~> 50 100 150 200 250 300 350 400 450
Abundance Scan 663 (6.524 min): W1100051 .D\data.ms
Raw
50
51 87,
8
164 202 283 446 490
m/z->
Abundance
Sub
m/z->
50
#29
Naphthalene
Concen: 81.41 ug/mL
RT: 6.524 min Scan# 663
Delta R.T. 0.000 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
Tgt Ion:128 Resp: 4009940
Ion Ratio Lower Upper
128 100
129 11.7 17.0 25.6#
127 13.4 35.1 52.7#
50 100 150 200 250 300 350 400 450
Scan 663 (6.524 min): W1100051 .D\data.ms (-624) (-)
1:
51
Abundance
3000000
2000000
1000000
164 202
283
6.524
50 100 150 200 250 300 350 400 450
446 490 o^
Time-> 6.45 6.50 6.55 6.60 6.65
Abundance Scan 966 (8.755 min): W1 00011 1.D (-959) (-)
Ref 50
o
1
115
63 RQ
38 i
^2
168193 231 281 327 387
m/z~> 50 100 150 200 250 300 350 400
Abundance Scan 789 (7.1 97 min): W1 1 00051 .D\data.ms
Raw en
\J\J
o
1
115
38 ,^89
\2
1 76 207 41 5
m/z~> 50 100 150 200 250 300 350 400
Abundance Scan 789 (7.197 min): W1 100051 .D\data.ms (-749) (-)
Sub
50
n
1
11
36 63 89
5
12
168196 415
m/z-> 50 100 150 200 250 300 350 400
#33
2 -Methylnaphthalene
Concen:
RT: 7.197
Delta R.T.
Lab File:
Acq: 28 Jan
Tgt Ion: 142
Ion Ratio
142 100
141 87.5
Abundance
200000
150000
100000
50000
0
i i i i
Time~> 7.15
6.31 ug/mL
min Scan# 789
0.004 min
W1100051.D
2011 5:56
pm
Resp: 199158
Lower Upper
69.7 104.5
7.197
A
/
A
7.20 7.25
W1100051.D W101810X-8270.M
EA# 0111-13 Page 32 of 112
Fri Jan 28 19:12:21 2011
Page
-------
Abundance
Scan 984 (8.861 min): W1000111.D (-978) (-)
#34
Ref 50
1<
115
39 63 89
i i j. il
i
m/z--> 50 1 00
£ 1-Methylnaphthalene
Concen: 5.09 ug/mL
RT: 7.299 min Scan# 808
Delta R.T. 0.005 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
162 195 221 271 295 327
150 200 250 300 T9t Ion:142 Re
sp: 155319
Abundance Scan 808 (7.299 min): W1 100051 .D\data.ms Ion Ratio Lower Upper
Raw 50
o
1'
39 63 89
j.i.i.. L.I.I...I,I,.III L .i ..i.
m/z-> 50 1 00
1'
5
r.J...
\2 142 100
141 89.9 72.5 108.7
Ab*f
| 165185207231 268
150 200 250 300
Abundance Scan 808 (7.299 min): W1 100051 .D\data.ms (-768) (-) 100000
Sub
50
o
V
39 6,3 89
.!... 1 I..J... ll.nl L .. L.h
m/z-> 50 1 00
Abundance Scan 11 25
Ref 50
76
50 j J.1P11?6
i'''
m/z--> 50 1 00
Abundance Scan 961
Raw 50
5°,,T,,1P1126
i ' ' '
m/z--> 50 1 00
1.
5
\2
50000
164 191 221 268 o
. ;
ft\
\
I. _
150 200 250 300 Time-> 7.25 7.30 7.35
(9.689 min): W1000111.D (-1118) (-) #47
15
'2 Acenaphthylene
Concen: 12.87 ug/mL
RT: 8.116 min Scan# 961
Delta R.T. -0
.012 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
177202 253 402
150 ' ' 200 ' 250 ' 30 0 ' ' 350 ' 400 T^ Ion:152 Resp: 612696
(8.1 16 min): W11 00051. D\data.ms Ion Ratio Lower Upper
15
12 152 100
151 22.7 15.0 22. 4#
153 13.1 10.2 15.2
Abundance
177 212 267 403
150 200 250 300 350 400 400000
Abundance Scan 961 (8.116 min): W1 100051 .D\data.ms (-924) (-)
Sub
50
o
76
50 j j 101 1 26
m/z-> 50 1 00
15
12
200000
176 212 281 403 o
8.116
A
// ^
j^f
150 200 250 300 350 400 Time-> 8.05 8.10
8.15 8.20
W1100051.D W101810X-8270.M
EA# 0111-13 Page 33 of 112
Fri Jan 28 19:12:21 2011
Page 7
-------
Abundance Scan 1243 (10.383 min): W1000111.D (-1233) (-)
Ref 50
o
1f
51 82 115
>6
19,8 232 264 355 429
m/z-> 50 1 00 1 50 200 250 300 350 400 450
Abundance Scan 1091 (8.811 min): W1 100051 .D\data.ms
Raw 50
1
39 83 115
ilili, ll, I Hi, Ll,ll
>6
195 281 450
\ — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — r
m/z-> 50 100 150 200 250 300 350 400 450
Abundance Scan 1091 (8.811 min): W1 100051 .D\data.ms (-1052) (-)
Sub
50
0
m/z-->
Abundance
Ref 50
o
1
83
, | | Lll |
>6
1 oc OQ-I 4^n
1 13O ^O 1 tOU
50 100 150 200 250 300 350 400 450
Scan 1412 (1 1 .376 min): W10001 1 1 .D (-1400) (-)
1
76 152
50 y j j98 126 j
'8
200 227 267 303 327
m/z~> 50 100 150 200 250 300
Abundance Scan 1275 (9.793 min): W1 100051 .D\data.ms
Raw 50
o
r
76 152
41 , j j98 126 j
'8
198220242 281 332
m/z~> 50 100 150 200 250 300
Abundance Scan 1275 (9.793 min): W1 100051 .D\data.ms (-1237) (-)
Sub
50
n
r
76 152
39 j ,,98 126 j
'8
201 223 250 332
m/z-> 50 100 150 200 250 300
#59
Fluorene
Concen: 4
RT: 8.811
Delta R.T.
Lab File:
Acq: 28 Jan
Tgt Ion: 166
Ion Ratio
166 100
165 93.7
167 15.2
Abundance
100000
50000
Time~> 5
#69
.48 ug/mL
min
Scan# 1091
0.000 min
W1100051 .D
2011
Resp
5:56 pm
: 152878
Lower Upper
75 .4 113 . 0
10.9 16 .3
8.$
I
11
3.70 8.75 8.80
Phenanthrene
Concen: 15
RT: 9.793
Delta R.T.
Lab File:
Acq: 28 Jan
Tgt Ion: 178
Ion Ratio
178 100
179 15.7
176 18.6
Abundance
600000
400000
200000
0
i i i i i i i
8.85
.89 ug/mL
min
Scan# 1275
-0.005 min
W1100051.D
2011
Resp
5:56 pm
: 795360
Lower Upper
12.5 18.7
15.0 22.4
9.t93
/ A \
JJ
Time~> 9.70 9.75 9
\l xx
.80 9.85
W1100051.D W101810X-8270.M
EA# 0111-13 Page 34 of 112
Fri Jan 28 19:12:22 2011
Page 9
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0
m/z-->
Abundance
Raw 50
0
m/z-->
Abundance
Sub 50
Scan 2023(1 4.966 mir
2^
113
39 75, || 151 20°
50 100 150 200
Scan 1799 (12.593m
2^
43 71 113 2QO
,,ll. .11, ill,,, ,,i,l ,1 1..5,° , , , lit ,j
50 100 150 200
Scan 1799 (12.593 min): \A
2^
59 87 1 \ 4 1 48 200
): W1 0001 11. D (-2016) (-) #85
!8 Chrysene
Concen: 1.32 ug/mL
RT: 12.593 min Scan# 1799
Delta R.T. -0.016 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
282 342 446
250 300 350 400 T9t Io
n):W1 100051. D\data.ms Ion R
•8 228 1
226 1
229
Abundance
50000
,1 281 341 415
1 40000
250 300 350 400
1 100051. D\data.ms (-1763) (-)
>Q OUUUU
20000
10000
758 9QR^9E;^E;E; 49Q n
n:228 Resp: 69071
atio Lower Upper
00
44 . 9 23 . 6 35 .4#
20.0 15 . 8 23 . 6
If
I /
I / v
I\L\
^vy\_
m/z->
50 100 150 200 250 300 350 400
Time->
12.55
12.60
12.65
Abundance
Ref 50
Scan 2393 (17.140 min): W1000111 .D (-2376) (-)
2$2
126
38 74
161 200 I 295 342 415
549
m/z-->
Abundance
Raw 50
50 100 150 200 250 300 350 400 450 500 550
Scan 2079 (14.089 min): W1100051 .D\data.ms
2$2
#88
Benzo(b)fluoranthene
Concen: 1.80 ug/mL m
RT: 14.089 min Scan# 2079
Delta R.T. -0.017 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
Tgt Ion:252 Resp: 87991
Ion Ratio Lower Upper
252 100
253 28.7 17.9 26.9#
125 16.0 7.7 11.5#
207
Abundance
3
73
126
n*
170,
i^V
312 355 415
m/z~> 50 100 150 200 250 300 350 400 450 500 550
Abundance Scan 2079 (14.089 min): W1100051 .D\data.ms (-2043) (-)
2$2
30000
20000
14^89
Sub
50
10000
126
39 .79 .1 J 1?9213
312 356 429
m/z->
50 100 150 200 250 300 350 400 450 500 550 Time-> 14.00 14.05 14.10
W1100051.D W101810X-8270.M
EA# 0111-13 Page 39 of 112
Fri Jan 28 19:12:24 2011
Page 14
-------
Abundance
Scan 2404 (17.204 min): W1000111 .D (-2398) (-)
#89
Ref 50
o
126
2!
50 83 j 1 57 200 Jh ,
2 Benzo (k) fluoranthene
Concen:
0.50 ug/mL
m
RT: 14.115 min Scan# 2084
Delta R.T.
Lab File:
-0.038 min
W1100051 .D
Acq: 28 Jan 2011 5:56 pm
284 323355387 431 489
m/z~> 50 100 150 200 250 300 350 400 450 T9t Ion:252 Resp: 24140
Abundance Scan 2084 (14.115 min): W1 100051. D\data.ms Ion Ratio
Raw 50
0
2!
207
^ 126
73 i
41 , 1 77
.1.1 ,1,1 Ml,.l,,.,L IlLlUl... 1 1, 1,1 1...
| | T | |f | ] \ } \ ] \ P|
ii i
I | |
2 252 100
253 104.5
125 58.3
Abundance
283 341 429 477
I I i i i ii i i i i i i i T i i i i i i OUUUU
m/z-> 50 100 150 200 250 300 350 400 450
Abundance Scan 2084 (14.115 min): W1 100051. D\data.ms (-2052) (-)
Sub
50
o
126
55 95 j 1 63 200 h
z 20000
10000
295 354 431 477 0
\
\
Lower Upper
14 . 9 22 .3#
8.8 13 .2#
14\115
~V_
— --^n
m/z-> 50 100 150 200 250 300 350 400 450 Time-> 14.10
"X
\
\
\
\ \
"^ — ^^ii^^— _
14.15
Abundance Scan 2502 (17.780 min): W1 00011 1 .D (-2489) (-) #90
Ref 50
o
125
43 74 j 174
2
222
>2 Benzo ( e ) pyrene
Concen:
0.77 ug/mL
RT: 14.553 min Scan# 2166
Delta R.T.
Lab File:
-0.028 min
W1100051.D
Acq: 28 Jan 2011 5:56 pm
282 327 413446 489
m/z~> 50 100 150 200 250 300 350 400 450 T9t Ion:252 Resp: 33693
Abundance Scan 2166 (14.553 min): W1 100051. D\data.ms Ion Ratio
Raw 50
0
2
207
.. 73 126
41 , 177
ill JLjil LilliLnJ i, ,,.. ,J, II
— "|i" | | ^ , | ) | | | , | | | ^
i
I i |
52 252 100
250 28.7
253 23.4
Abundance
,,,2F 341 402 70000
I r | i i | I I — r I I I — I — I — i ii — i — i — i — i — i — i — i — r fcwwww
m/z~> 50 100 150 200 250 300 350 400 450
Abundance Scan 2166 (14.553 min): W1 100051. D\data.ms (-2134) (-) 150oo
Sub
50
126
44 82 , 1R9193
2
i2
10000
5000
987 398 3R8 41 Fl n
=— ^=^s-
Lower Upper
23.0 34.4
17.8 26.8
14.553
/s
/
A\ L
If \\ n
-^J/ "^i~^^Ls
r
i
/'
m/z->
50 100 150 200 250 300 350 400 450
Time-> 14.50 14.55 14.60
W1100051.D W101810X-8270.M
EA# 0111-13 Page 40 of 112
Fri Jan 28 19:12:24 2011
Page 15
-------
Abundance Scan 2522 (17.897 min): W1 00011 1 .D (-251 1) (-) #91
Ref 50
m/z-->
2J
126
48 86 174211. ,
50 100 150 200 25
>2 Benzo (a) pyrene (CCC)
Concen: 1.06 ug/mL
RT: 14.650 min Scan# 2184
Delta R.T. -0 . 027 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
295327 387 430 503
o"30o"350 40o"450 500 T9fc I°n:252 Resp: 47912
Abundance Scan 2184 (14.650 min): W1 100051. D\data.ms Ion Ratio Lower Upper
Raw 50
0
2J
207
73 1 3
41 I 147 I
...(i A J r.v ., i | ,, i t I i
i i | i i i i i i i i i i i i i i [ i i I.
i2 252 100
253 22 . 7 16 . 6 25.0
Abundance
.... I300 341 415 70000
i i i i i i i i | i i i i | i i i i | i i i i | i
m/z-> 50 100 150 200 250 300 350 400 450 500
Abundance Scan 2184 (14.650 min): W1 100051. D\data.ms (-2150) (-) 150oo
Sub
50
2J
126
53 87 169 209il
i2
10000
5000
300 341 43? n
14/1350
"i
I /\\
~\ \ i \\ ^
v\ // Vv f
50 100 150 200 250 300 350 400 450 500 Time->
14.60 14.65 14.70
Abundance
Ref 50
Scan 3015 (20.794 min): W1000111 .D (-2994) (-)
2t6
138
39 92
191224
311 343
415446 503
m/z~> 50 100 150 200 250 300 350 400 450
Abundance Scan 2579 (16.760 min): W1100051.D\data.ms
207
276
500
#93
Indeno(1,2,3-cd)pyrene
Concen: 0.74 ug/mL
RT: 16.760 min Scan# 2579
Delta R.T. -0.066 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
Tgt Ion:276 Resp: 32792
Ion Ratio Lower Upper
276 100
138 27.1 21.4 32.2
Raw 50
73
Abundance
137
41
174,,
307341 401 475
m/z~> 50 100 150 200 250 300 350 400 450 500
Abundance Scan 2579 (16.760 min): W1100051 .D\data.ms (-2552) (-)
2t6
Sub
10000
5000
50
138
41 74 107,1 1T4 222
, I ,h M I. ,ll , n, I I ill .fill.,. I 111.,. I, , ,
325358 415 475
16/7,60
\
m/z->
50 100 150 200 250 300 350 400 450 500 Time->
16.70
16.80
W1100051.D W101810X-8270.M
EA# 0111-13 Page 41 of 112
Fri Jan 28 19:12:24 2011
Page 16
-------
Abundance Scan 3157 (21 .628 min): W1 00011 1 .D (-3137) (-) #95
Ref 50
o
2;
138
55 91 , 187222 ,
6 Benzo (g, h, i) perylene
Concen: 0.91 ug/mL
RT: 17.337 min Scan# 2687
Delta R.T. -0.076 min
Lab File: W1100051.D
Acq: 28 Jan 2011 5:56 pm
323 368 416 489 549
m/z~> 50 100 150 200 250 300 350 400 450 500 550 T9t Ion:276 Resp: 33051
Abundance Scan 2687 (17.337 min): W1 100051. D\data.ms Ion Ratio Lower Upper
Raw 50
m/z-->
2(h7 276 100
138 28.3 21.2 31.8
276 277 25.3 19.3 28.9
73
137
Abundance
3f 1 429 1 0000
i — | 'liii'i'i'Yy^y'VT'T't r I' "i"'"i 1" I i |"" i i "i '^'|'Y | — \—\ — i — r~i — i — r~i — i — r~i — i — r~i — i — i — r~i — i — r~i — i — r~i — i — i — r~r
50 100 150 200 250 300 350 400 450 500 550
Abundance Scan 2687 (1 7.337 min): W1 100051 .D\data.ms (-2662) (-)
Sub 50
21
137 207
35 73 171
6
5000
329 369 461 n
17 837
M
M
I
/ \
.M [
f\\
^ j/ V^_^
m/z->
50 100 150 200 250 300 350 400 450 500 550 Time->
17.30 17.40
W1100051.D W101810X-8270.M
EA# 0111-13 Page 42 of 112
Fri Jan 28 19:12:24 2011
Page 17
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100049.D
28 Jan 2011 4 :55 pm
tdd
0111-13 MB
9 Sample Multiplier: 1
Quant Time Jan 28 18:39:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Abundance
5600000
5400000
5200000
5000000
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
n
(j
':
3
s
A
1
0
_o
o
b
5
1
"o U)
¥
° &
O U.)
[
3 7
-
•5
5
1
1, ,
CL
C
'
o
T5
^ 0
^ 0
OD -C
-9 :
0
0
05
-C
Q.
05
-z.
0
3
i-O
J
0
N
•
a
D
iz
'o
05
O
'o
C
0
CQ
LjUJi
(0
•c^
f-
0
Q_
LQ
0
0
iZ
CM
^»__.-^_T__-_
r~^. ^
|-
|
IS
i
0
0
0
b
=3
LL
TIC: W1 100049.D\data.ms
_
CM
T5
0
— C
.-^ 0
^ U)
C ) &"
I
c
(0
_
~
3
0_
5
0
Q.
P
.
h
C£
^
D
5
3
f
CM"
,
' 0
^r-
it
°?
cc
0tD
H
l__Ju________«i
_„ u ^_
g
CM
0
C
0
I
. . ...
_________^_
Time~> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Jan 28 18:39:22 2011
DO-, 11.0Q,, 12.QO.j-13.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\janll\W012811A\
W1100049.D
28 Jan 2011 4 :55 pm
tdd
0111-13 MB
9 Sample Multiplier: 1
(QT Reviewed)
Quant Time Jan 28 18:39:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.290
.497
.255
. 767
. 561
.751
152
136
164
188
240
264
522090
1845188
1083822
1974624
1995220
1733409
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
-0 .
-0.
.00
. 00
. 00
. 00
. 01
.01
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.157 112 218019
5) Phenol-d5 (surr) 4.884 99 261447
19) Nitrobenzene-d5 (surr) 5.787 82 226918
39) 2-Fluorobiphenyl (surr) 7.539 172 539767
57) Fluorene-dlO (surr) 8.778 176 514511
65) 2,4,6-Tribromophenol (... 9.051 330 80797
75) Pyrene-dlO (surr) 11.225 212 828814
77) Terphenyl-dl4 (surr) 11.343 244 634438
Target Compounds
15.32 ug/mL
15.79 ug/mL
13.96 ug/mL
16.11 ug/mL
17.29 ug/mL
17.87 ug/mL
20.14 ug/mL
19.37 ug/mL
-0.02
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.01
0.00
0.01
Qvalue
Pyridine 0.000
Phenol (CCC) 0.000
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 0.000
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 0.000
Benzpifcacid 6.123
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
105
N.D.
N.D.
N.D.
N.D.
N.D^
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
2.52 ug/mL
N.D. d
N.D. d
d
,4-Tliuhll
Naphthalene
u . u u u
0 . 000
4-Chlun
TJovantLL
u . u u u
n nrm
N.D.
N.D. d
N.D. d
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D.
2-Methylnaphthalene
1-Methylnaphthalene
0.000
0.000
n n n n
N.D.
N.D.
0.000
0 . 000
0 . 000
0 . 000
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
", 2 -Diiiiti-obe
0111-13 Page44offl§-
W101810X-8270.M Fri Jan 28 18:39:21 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100049.D
28 Jan 2011 4 :55 pm
tdd
0111-13 MB
9 Sample Multiplier: 1
Quant Time Jan 28 18:39:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response
Cone Units Dev(Min)
N.D.
Acenaphthylene
3 Mitroaniline
Acenaphthene
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
0.000
N.D.
N.D.
N.D.
N.D. d
Tetrachlorophenol
Fluorene
i-Niti-oaiiiliiie
0 . 000
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D
N.D
N.D
N.D
Phenanthrene
Anthracene
0 . 000
0 . 000
7*4-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
N.D. d
0.000
N.D.
N.D.
84)
85)
Pyrene
0.000
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipate_
bis(2-Eth\
u . u u u
0 . 000
Benzo (a) anthracene
Chrysene
0.000
0.000
ILyX^jliLI id. _LciLc (CCC) 0 . 000
88) Benzo(b)fluoranthene 0.000
89) Benzo(k)fluoranthene 0.000
90) Benzo(e)pyrene 0.000
91) Benzo(a)pyrene (CCC) 0.000
92) Perylene 0.000
93) Indeno(1,2,3-cd)pyrene 0.000
94) Dibenz(a,h)anthracene 0.000
95) Benzo(g,h,i)perylene 0.000
N.D. d
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 45 of 112
W101810X-8270.M Fri Jan 28 18:39:21 2011
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100045.D
28 Jan 2011 2:52 pm
tdd
CH2C12 System Blank (Scan)
5 Sample Multiplier: 1
Quant Time Jan 28 18:42:41 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Abundance
5400000
5200000
5000000
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
n
TIC: W1 100045.D\data.ms
CO ° C
-o ZI v
0 1
0
™
^ £
S Q.
"9 2
c
0
N
1
0
_0
o
b
^
••
jj
W — , — •, — , — , — , — X
)
I _
i ^?
! CM
- 1
C
0
_!"
o
V
CM
0
0
Q.
^
L___^ __™^__-___— —
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270 .M Fri Jan 28 18:44:31 2011
1
„ 1 ZOO.-13.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100045.D
28 Jan 2011 2 : 52 pm
tdd
CH2C12 System Blank (Scan)
5 Sample Multiplier: 1
Quant Time Jan 28 18:42:41 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1) 1,4-Dichlorobenzene-d4... 5.290
21) Naphthalene-d8 (I) 6.497
36) Acenaphthene-dlO (I) 8.260
64) Phenanthrene-dlO (I) 9.767
78) Chrysene-dl2 (I) 12.561
87) Perylene-dl2 (I) 14.746
System Monitoring Compounds
4) 2-Fluorophenol (surr) 0.000
5) Phenol-d5 (surr) 0.000
19) Nitrobenzene-d5 (surr) 0.000
39) 2-Fluorobiphenyl (surr) 0.000
57) Fluorene-dlO (surr) 0.000
65) 2,4,6-Tribromophenol (... 0.000
75) Pyrene-dlO (surr) 0.000
77) Terphenyl-dl4 (surr) 0.000
Target Compounds
152 554892
136 1981163
164 1122172
188 1929188
240 1719405
264 1512053
40.00 ug/mL 0.00
40.00 ug/mL 0.00
40.00 ug/mL 0.00
40.00 ug/mL 0.00
40.00 ug/mL -0.01
40.00 ug/mL -0.02
112
99
82
172
176
330
212
244
0.00
0.00 ug/mL
0.00 ug/rnL
0.00 ug/mL
0.00
0.00
0.00 ug/mL
0.00
Qvalue
Pyridine 0.000
Phenol (CCC) 0.000
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 0.000
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 0.000
Benzpifcacid 0.000
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
N.D.
N.D.
N.D.
N.D.
N.D.^
,4-Tliuhll
Naphthalene
4-Chlun
TJovan>-i1 ,
u . u u u
n nrm
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D.
2-Methylnaphthalene
1-Methylnaphthalene
0.000
0.000
n n n n
N.D.
N.D.
0.000
0 . 000
0 . 000
0 . 000
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
", 2 -Diiiiti-obe
0111-13 Page47offl§-
W101810X-8270.M Fri Jan 28 18:44:30 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100045.D
28 Jan 2011 2:52 pm
tdd
CH2C12 System Blank (Scan)
5 Sample Multiplier: 1
Quant Time Jan 28 18:42:41 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response
Cone Units Dev(Min)
N.D.
Acenaphthylene
3 Nitroanilinc
Acenaphthene
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
0.000
Tetrachlorophenol
Fluorene
i-Niti-oaiiiliiie
0 . 000
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D
N.D
N.D
N.D
Phenanthrene
Anthracene
0 . 000
0 . 000
7*4-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
N.D.
0.000
N.D.
N.D.
84)
85)
Pyrene
0.000
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipate_
bis(2-Eth\
u . u u u
0 . 000
Benzo (a) anthracene
Chrysene
0.000
0.000
ILyX^jliLI id. _LciLc (CCC) 0 . 000
88) Benzo(b)fluoranthene 0.000
89) Benzo(k)fluoranthene 0.000
90) Benzo(e)pyrene 0.000
91) Benzo(a)pyrene (CCC) 0.000
92) Perylene 0.000
93) Indeno(1,2,3-cd)pyrene 0.000
94) Dibenz(a,h)anthracene 0.000
95) Benzo(g,h,i)perylene 0.000
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 48 of 112
W101810X-8270.M Fri Jan 28 18:44:30 2011
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100043.D
28 Jan 2011 1:48 pm
tdd
0111-13 LCS
3 Sample Multiplier: 1
Quant Time Jan 28 14:10:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Abundance
5600000
5400000
5200000
5000000
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
TIC: W1100043.D\data.ms
Cfl
•?
i
5
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Jan 28 18:48:40 2011
140°
Page : 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\janll\W012811A\
W1100043.D
28 Jan 2011 1:48 pm
tdd
0111-13 LCS
3 Sample Multiplier: 1
(QT Reviewed)
Quant Time Jan 28 14:10:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.290
. 503
.260
. 772
. 566
.756
152
136
164
188
240
264
553789
1980939
1132651
1983150
1750154
1422438
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.157 112 254174
5) Phenol-d5 (surr) 4.884 99 307408
19) Nitrobenzene-d5 (surr) 5.787 82 293850
39) 2-Fluorobiphenyl (surr) 7.539 172 602034
57) Fluorene-dlO (surr) 8.778 176 571950
65) 2,4,6-Tribromophenol (... 9.051 330 86854
75) Pyrene-dlO (surr) 11.231 212 781120
77) Terphenyl-dl4 (surr) 11.343 244 587552
Target Compounds
16.83 ug/mL
17.51 ug/mL
17.05 ug/mL
17.19 ug/mL
18.39 ug/mL
19.13 ug/mL
18.90 ug/mL
17.86 ug/mL
-0.02
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.01
0.00
0.01
Qvalue
Pyridine 3.228
Phenol (CCC) 4.900
Aniline 4.980
bis(2-Chloroethyl)ether 5.002
2-Chlorophenol 5.103
1,3-Dichlorobenzene 5.247
1,4-Dichlorobenzene (CCC) 5.306
Benzyl alcohol 5.375
1,2-Dichlorobenzene 5.456
2-Methylphenol 5.450
bis(2-Chloroisopropyl)... 5.48j
3/4-Methylphenol 5x^84
N-Nitrso-di-n-propylam. .^^^^.611
Hexachloroethane ^s^ 5.776
Nitrobenzene ^s^ 5.808
Isophorone ^s^ 6.017
2 , 4-Dimethy^Uprnenol 6.097
2-Nitropte£nol (CCC) 6.113
Benzpifcacid 6.145
(2-Chloroethoxy)met... 6.188
"2,4-Dichlorophenol (CCC) 6.337
,4-Tliuhll
Naphthalene
6.433
6 . 524
128
4-Chlun
TJovan>-i1 ,
r*fl-
1 o 4 9 0 o
180891
285772
73851
167610
276969
309519
198299
217782
213035
264171
184295
112433
274097
475222
214724
160124
226424
281428
201445
274168
840530
53742
i £-?T £ a
15 .46
10 .20
15 . 59
8.91
15.47
ug/mL#
ug/mL#_
ugA
>^7r
ug/mL
y.g/mL
ug/mL
ug/mL#
y.g/mL
y.g/mL
lig/mL#
ug/mL
lig/mL#
y.g/mL
y.g/mL
y.g/mL
ug/mL#
y.g/mL
lig/mL#
y.g/mL
y.g/mL
TTT
|j.y / LLIJ_I
y.g/mL
M-y/
r /ml.
mothylphono. . .
-i^
1 Q1 p f p
2-Methylnaphthalene
1-Methylnaphthalene
7.197
7.304
543266
523921
i a i i Q A
17.78
17.73
1C *"? Q
ug/mL
ug/mL
y.g/mL
7.464
7 . 502
7 . 694
7 . 769
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
523
88899
628667
101419
143538
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
., 2-Diiiifei.-eibi!
W101810X-8270.M Fri Jan 28 18:48:39 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100043.D
28 Jan 2011 1:48 pm
tdd
0111-13 LCS
3 Sample Multiplier: 1
Quant Time Jan 28 14:10:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon
Response
828230
Cone Units Dev(Min)
47) Acenaphthylene
4-8-)—3 Mitroanilinc
8.121
0.17D
ug/mL
ug/mLtt
518942
51072
17 . 81
11 .30
8 .298
8 .405
8.463
77340
189414
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
18.38
17.30
16.84
19.24
Tetrachlorophenol
149224
146993
636394
59) Fluorene
66-)—4-NitL-oaiiiliiie
615875
123003
61) 4,6-Dinitro2-methylphenol
62) N-Nitrosodiphenylamine...
63) Azobenzene
66) 4-Bromophenyl-ph
8 . 827
8.891
ug/mL
ug/mL#
ug/mL
ug/mL
552500
185963
191523
9.275
9.382
ug/mL
ug/mL
Phenanthrene
70) Anthracene
2) Di n butylphthalat
ug/mL
ug/mL
73) Fluoranthene (CCC)
76) Pyrene
49) Acenaphthene (CCC)
5-9-)—2,1 Dinitrophonol—fS
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-
55) 2,3,4,6-
56) DiethlwstetftaTate
7 9")Bu.Lylbt:iizy IpliLlidld L
80)
81)
82)
834-
84)
85)
88)
89)
90)
91)
92)
93)
94)
95)
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethvl 1ir—/1 ^ jjhl h i "
~l n i _ | 'i -{ r-i'h 1 ^^-K-^^V-iQ-n <-? -1 H -i T-I c=±
Benzo (a) anthracene
Chrysene
B1 i Till Hi /^-i^-i^-i\
_L 11 <_>^ i_ y -L^ii uiid-Ld i_ cr v *— *— *— 1
Benzo (b) fluoranthene
Benzo (k) fluoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
11 .
-U-
12 .
1 "~>
12.
12.
14 .
14 .
14 .
14 .
14.
16.
16.
17.
. 824
.a 18-
.406
/]_£. C
.550
.598
. 094
. 131
.559
. 655
.794
.770
.776
.347
212
-«?-
149
O R O
228
228
252
252
252
252
252
276
278
276
c;c;gn97 __
379624
599389
A n r\ Q Q c.
809408
745983
y ~D _> / y _>
689771
698187
670300
634625
643038
594964
498597
478483
^9-
22
22
T C.
19
18
JL JL :
19
20
21
19
18
18
18
18
. 00
. 60
A "3
.16
.74
.39
. 10
. 15
.40
.84
.39
.27
.14
ug/mL#
ug/mL#
ug/mL#
urr 7mL
y.g/mL
y.g/mL
|_Ly / LLiL-fr
y.g/mL
y.g/mL
y.g/mL
ug/mL
y.g/mL
y.g/mL
y.g/mL
y.g/mL
100
100
100
]_nn
98
99
100
98
95
99
98
99
95
97
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 51 of 112
W101810X-8270.M Fri Jan 28 18:48:39 2011
Page: 2
-------
Calibration
Curve Chromatograms
(Scan)
i-'J
-------
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
# Name
2) N-Nitrosodimethylamine
3) Pyridine
6) Phenol (CCC)
7) Aniline
8) bis(2-Chloroethyl)ether
9) 2-Chlorophenol
10) 1,3-Dichlorobenzene
11) 1,4-Dichlorobenzene (CCC)
12) Benzyl alcohol
13) 1,2-Dichlorobenzene
14) 2-Methylphenol
15) bis(2-Chloroisopropyl) ether
16) 3/4-Methylphenol
17) N-Nitrso-di-n-propylamine (SPCC)
18) Hexachloroethane
20) Nitrobenzene
22) Isophorone
23) 2,4-Dimethylphenol
24) 2-Nitrophenol (CCC)
25) Benzole acid
26) bis(2-Chloroethoxy)methane
27) 2,4-Dichlorophenol (CCC)
28) 1,2,4-Trichlorobenzene
29) Naphthalene
30) 4-Chloroaniline
31) Hexachlorobutadiene (CCC)
32) 4-Chloro-3 methylphenol (CCC)
33) 2-Methylnaphthalene
34) 1-Methylnaphthalene
35) Hexachlorocyclopentadiene (SPCC)
37) 2,4,6-Trichlorophenol (CCC)
38) 2,4,5-Trichlorophenol
40) 2-Chloronaphthalene
41) 2-Nitroaniline
42) 1,4-Dinitrobenzene
43) Dimethylphthalate
44) 1,3-Dinitrobenzene
45) 2,6-Dinitrotoluene
46) 1,2-Dinitrobenzene
47) Acenaphthylene
48) 3-Nitroaniline
49) Acenaphthene (CCC)
50) 2,4-Dinitrophenol (SPCC)
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-Tetrachlorophenol
55) 2,3,4,6-Tetrachlorophenol
56) Diethlyphthalate
58) 4-Chlorophenyl-phenylether
59) Fluorene
60) 4-Nitroaniline
61) 4,6-Dinitro2-methylphenol
62) N-Nitrosodiphenylamine (CCC)
63) Azobenzene
66) 4-Bromophenyl-phenylether
67) Hexachlorobenzene
68) Pentachlorophenol (CCC)
69) Phenanthrene
70) Anthracene
71) Carbazole
72) Di-n-butylphthalate
73) Fluoranthene (CCC)
74) Benzidine
76) Pyrene
79) Butylbenzylphthalate
80) 3,3-Dimethylbenzidine
81) bis(2-ethylhexyl)adipate
82) bis(2-Ethylhexyl)phthalate
83) 3,3'-Dichlorobenzidine
84) Benzo(a)anthracene
LID
5
10
20
50
80
120
160
••
SCAN
gcms22pg1
25
0.765
1.22
1.41
0.610
0.802
1.21
1.41
1.43
0.611
1.36
0.918
1.02
1.16
0.793
0.504
1.21
0.581
0.221
0.168
0.0945
0.369
0.214
0.344
1.04
0.106
0.200
0.202
0.636
0.619
0.182
0.333
0.319
1.11
0.241
0.134
1.22
0.168
0.275
0.122
1.67
0.260
1.08
0.0615
0.140
0.312
1.49
0.253
0.268
1.20
0.615
1.18
0.226
0.117
1.01
1.09
0.203
0.212
0.0781
0.964
0.936
0.799
1.00
0.951
0.402
1.06
0.405
0.536
0.377
0.525
0.287
0.987
Response Factor Report
FileName Acq. Date
W1 002873. D 10/18/10 17:56
W1 002874. D 10/18/10 18:26 Inst:
W1 002875. D 10/18/10 18:55
W1 002876. D 10/18/10 19:25 Curve:
W1002877.D 10/18/10 19:54
W1002878.D 10/18/10 20:24
W1 002879. D
SCAN
gcms22pg1
25
0.750
1.27
1.40
0.609
0.798
1.22
1.41
1.44
0.652
1.36
0.906
0.992
1.16
0.809
0.505
1.19
0.598
0.233
0.177
0.124
0.365
0.247
0.342
1.02
0.109
0.201
0.218
0.630
0.606
0.177
0.356
0.352
1.11
0.263
0.154
1.24
0.187
0.286
0.131
1.69
0.278
1.05
0.0781
0.160
0.342
1.45
0.270
0.279
1.19
0.611
1.17
0.238
0.138
1.02
1.05
0.210
0.214
0.0932
0.953
0.928
0.795
1.07
0.993
0.387
1.08
0.435
0.557
0.397
0.584
0.269
0.969
Wiley
W101810X-8270.M
10/18/10 20:54
SCAN
gcms22pg1
25
0.746
1.26
1.36
0.599
0.796
1.19
1.35
1.37
0.658
1.28
0.897
0.981
1.14
0.795
0.493
1.17
0.572
0.273
0.178
0.172
0.353
0.234
0.327
0.978
0.106
0.193
0.230
0.610
0.594
0.197
0.349
0.353
1.05
0.268
0.161
1.16
0.188
0.276
0.126
1.64
0.270
1.00
0.108
0.182
0.342
1.39
0.275
0.286
1.15
0.590
1.13
0.251
0.166
0.970
1.05
0.203
0.205
0.0988
0.898
0.910
0.759
1.01
0.905
0.430
0.980
0.438
0.578
0.397
0.609
0.276
0.947
SCAN
gcms22pg1
25
0.776
1.30
1.31
0.603
0.794
1.20
1.36
1.38
0.643
1.30
0.861
0.982
1.13
0.802
0.512
1.15
0.593
0.305
0.184
0.216
0.355
0.262
0.328
0.957
0.109
0.196
0.256
0.615
0.594
0.218
0.364
0.364
1.05
0.283
0.184
1.20
0.206
0.290
0.137
1.62
0.292
1.02
0.150
0.222
0.368
1.40
0.310
0.312
1.18
0.608
1.16
0.288
0.210
1.01
1.09
0.203
0.211
0.121
0.904
0.915
0.788
1.04
0.947
0.496
1.01
0.451
0.639
0.404
0.645
0.274
0.963
SCAN
gcms22pg1
25
0.804
1.34
1.30
0.601
0.789
1.22
1.39
1.41
0.653
1.32
0.869
0.960
1.14
0.806
0.523
1.13
0.593
0.328
0.192
0.233
0.360
0.271
0.343
0.967
0.111
0.209
0.260
0.620
0.599
0.217
0.380
0.382
1.07
0.295
0.194
1.25
0.219
0.308
0.149
1.62
0.303
1.04
0.170
0.237
0.398
1.42
0.337
0.338
1.22
0.638
1.18
0.305
0.237
1.04
1.08
0.212
0.222
0.134
0.906
0.914
0.812
1.05
0.990
0.557
1.06
0.473
0.677
0.411
0.659
0.284
0.986
SCAN
gcms22pg1
25
0.779
1.29
1.24
0.589
0.756
1.17
1.35
1.37
0.644
1.28
0.829
0.921
1.15
0.801
0.514
1.09
0.586
0.335
0.190
0.258
0.350
0.280
0.338
0.912
0.111
0.209
0.273
0.618
0.599
0.233
0.374
0.380
1.03
0.282
0.194
1.17
0.214
0.296
0.141
1.52
0.297
1.01
0.180
0.238
0.375
1.35
0.333
0.333
1.12
0.625
1.12
0.292
0.235
0.982
0.993
0.217
0.229
0.142
0.861
0.875
0.771
0.947
0.914
0.573
0.962
0.444
0.680
0.382
0.610
0.331
0.961
SCAN
gcms22pg1
25
0.771
1.28
1.25
0.581
0.741
1.16
1.32
1.34
0.653
1.26
0.856
0.913
1.16
0.790
0.506
1.10
0.565
0.322
0.190
0.258
0.337
0.277
0.331
0.868
0.107
0.207
0.270
0.590
0.566
0.222
0.378
0.391
1.00
0.285
0.201
1.19
0.221
0.305
0.147
1.49
0.304
1.00
0.195
0.248
0.391
1.33
0.354
0.341
1.12
0.637
1.13
0.307
0.253
1.01
0.982
0.218
0.228
0.144
0.834
0.833
0.757
0.883
0.877
0.549
0.911
0.448
0.686
0.392
0.611
0.348
0.947
Average RF
0.770
1.28
1.32
0.599
0.782
1.19
1.37
1.39
0.645
1.31
0.876
0.966
1.15
0.799
0.508
1.15
0.584
0.288
0.183
0.194
0.356
0.255
0.336
0.963
0.108
0.202
0.244
0.617
0.597
0.206
0.362
0.363
1.06
0.274
0.175
1.20
0.201
0.291
0.136
1.61
0.286
1.03
0.135
0.204
0.361
1.40
0.305
0.308
1.17
0.618
1.15
0.272
0.194
1.01
1.05
0.210
0.217
0.116
0.903
0.902
0.783
1.00
0.940
0.485
1.01
0.442
0.622
0.394
0.606
0.295
0.966
%RSD
2.53%
2.86%
4.92%
1.76%
3.03%
2.03%
2.39%
2.74%
2.46%
3.08%
3.59%
3.88%
1.08%
0.88%
1.83%
3.86%
2.07%
16.2%
4.80%
33.6%
3.01%
9.58%
2.16%
6.20%
1.99%
3.13%
11.3%
2.43%
2.72%
10.3%
4.76%
6.72%
3.77%
6.61%
14.4%
2.61%
9.86%
4.50%
7.46%
4.55%
6.00%
2.83%
38.9%
21.0%
8.45%
3.93%
12.7%
9.89%
3.42%
2.75%
2.19%
12.2%
27.6%
2.42%
4.20%
3.21%
4.23%
22.4%
5.10%
3.98%
2.72%
6.64%
4.61%
16.1%
6.18%
4.65%
10.3%
3.06%
7.17%
10.5%
1.70%
Pass/Fail
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
EA# 0111-13 Page 53 of 112
-------
Sample ID LID
SSTD005-SCANgcms22pg125 5
SSTD010-SCANgcms22pg125 10
SSTD020-SCANgcms22pg125 20
SSTD050-SCANgcms22pg125 50
SSTD080-SCANgcms22pg125 80
SSTD120-SCAN gcms22pg125 120
SSTD160-SCAN gcms22pg125 160
Response Factor Report
FileName Acq. Date
W1002873. D 10/18/10 17:56
W1002874. D 10/18/10 18:26
W1002875. D 10/18/10 18:55
W1002876. D 10/18/10 19:25
W1002877. D 10/18/10 19:54
W1002878.D 10/18/10 20:24
W1002879.D 10/18/10 20:54
Inst:
Wiley
Curve: W101810X-8270.M
n Name
85) Chrysene
86) Di-n-octylphthalate (CCC)
88) Benzo(b)fluoranthene
89) Benzo(k)fiuoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) lndeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
SPCC Average RF must be > 0.05
CCC %RSD must be <= 30%
Other %RSD<= 15%
Average %RSD <= 15%
No %RSD > 50%
SCAN
gcms22pg1
25
0.936
0.760
0.943
0.945
0.864
0.859
0.956
0.879
0.723
0.744
SCAN
gcms22pg1
25
0.933
0.888
0.967
0.983
0.881
0.882
0.966
0.842
0.701
0.707
SCAN
gcms22pg1
25
0.887
0.977
0.920
0.952
0.857
0.882
0.933
0.966
0.808
0.815
SCAN
gcms22pg1
25
0.913
1.06
0.973
0.969
0.874
0.916
0.942
0.984
0.828
0.812
SCAN
gcms22pg1
25
0.922
1.06
1.09
1.01
0.938
0.985
1.00
0.937
0.793
0.747
SCAN
gcms22pg1
25
0.892
1.00
1.01
1.00
0.906
0.951
0.962
0.909
0.781
0.711
SCAN
gcms22pg1
25
0.885
0.963
1.10
0.979
0.918
0.962
0.963
0.851
0.737
0.657
Average RF
0.910
0.957
1.00
0.977
0.891
0.920
0.960
0.910
0.767
0.742
%RSD
2.40%
11.0%
7.04%
2.46%
3.39%
5.17%
2.07%
6.09%
6.15%
7.72%
Pass/Fail
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Average % 6.7%
EA# 0111-13 Page 54 of 112
-------
Relative Retention Time Report
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
# Name
2) N-Nitrosodimethylamine
3) Pyridine
6) Phenol (CCC)
7) Aniline
8) bis(2-Chloroethyl)ether
9) 2-Chlorophenol
10) 1,3-Dichlorobenzene
11) 1,4-Dichlorobenzene (CCC)
12) Benzyl alcohol
13) 1,2-Dichlorobenzene
14) 2-Methylphenol
15) bis(2-Chloroisopropyl) ether
16) 3/4-Methylphenol
17) N-Nitrso-di-n-propylamine (SPCC)
18) Hexachloroethane
20) Nitrobenzene
22) Isophorone
23) 2,4-Dimethylphenol
24) 2-Nitrophenol (CCC)
25) Benzole acid
26) bis(2-Chloroethoxy)methane
27) 2,4-Dichlorophenol (CCC)
28) 1,2,4-Trichlorobenzene
29) Naphthalene
30) 4-Chloroaniline
31) Hexachlorobutadiene (CCC)
32) 4-Chloro-3 methylphenol (CCC)
33) 2-Methylnaphthalene
34) 1-Methylnaphthalene
35) Hexachlorocyclopentadiene (SPCC)
37) 2,4,6-Trichlorophenol (CCC)
38) 2,4,5-Trichlorophenol
40) 2-Chloronaphthalene
41) 2-Nitroaniline
42) 1,4-Dinitrobenzene
43) Dimethylphthalate
44) 1,3-Dinitrobenzene
45) 2,6-Dinitrotoluene
46) 1,2-Dinitrobenzene
47) Acenaphthylene
48) 3-Nitroaniline
49) Acenaphthene (CCC)
50) 2,4-Dinitrophenol (SPCC)
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-Tetrachlorophenol
55) 2,3,4,6-Tetrachlorophenol
56) Diethlyphthalate
58) 4-Chlorophenyl-phenylether
59) Fluorene
60) 4-Nitroaniline
61) 4,6-Dinitro2-methylphenol
62) N-Nitrosodiphenylamine (CCC)
63) Azobenzene
66) 4-Bromophenyl-phenylether
67) Hexachlorobenzene
68) Pentachlorophenol (CCC)
69) Phenanthrene
70) Anthracene
71) Carbazole
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.97
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Acq. Date
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
17:56
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.97
0.99
1.00
1.00
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Inst:
Curve:
SCAN
gcms22pg1
25
0.60
0.61
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.97
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
0.61
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.01
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.07
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
SCAN
gcms22pg1
25
0.61
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.96
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.01
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.07
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Average
RRT
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.02
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
EA# 0111-13 Page 55 of 112
-------
Relative Retention Time Report
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
# Name
72) Di-n-butylphthalate
73) Fluoranthene (CCC)
74) Benzidine
76) Pyrene
79) Butylbenzylphthalate
80) 3,3-Dimethylbenzidine
81) bis(2-ethylhexyl)adipate
82) bis(2-Ethylhexyl)phthalate
83) 3,3'-Dichlorobenzidine
84) Benzo(a)anthracene
85) Chrysene
86) Di-n-octylphthalate (CCC)
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) lndeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Acq. Date
10/18/10 17:56
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Inst:
Curve:
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Average
RRT
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
= RRT outside 0.06 unit RRT window
EA# 0111-13 Page 56 of 112
-------
Internal Standards Retention Time Report
#
1)
21)
36)
64)
78)
87)
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
= ISTD retention time outside of 20 sec (0.33 min) window
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.89
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.89
Acq. Date
10/18/10 17:56
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.90
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.85
12.66
14.90
Inst:
Curve:
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.85
12.67
14.90
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.86
12.67
14.90
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.86
12.67
14.90
Average
5.38
6.59
8.34
9.85
12.67
14.90
EA# 0111-13 Page 57 of 112
-------
Internal Standards Area Report
#
1)
21)
36)
64)
78)
87)
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-dS (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
= ISTD response outside of 40% window
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
694,978
2,450,968
1,342,042
2,316,172
2,072,984
1,889,069
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
706,693
2,486,145
1,339,110
2,294,140
2,146,192
1,945,018
Acq. Date
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
720,068
2,542,711
1,390,154
2,354,525
2,151,499
2,135,523
17:56
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
655,591
2,346,284
1,314,587
2,336,676
2,279,787
2,168,681
Inst:
Curve:
SCAN
gcms22pg1
25
662,846
2,259,727
1,246,926
2,303,538
2,250,870
1,934,135
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
708,059
2,432,537
1,402,728
2,472,354
2,393,551
2,217,223
SCAN
gcms22pg1
25
673,532
2,366,580
1 ,358,679
2,516,253
2,291,395
1 ,886,569
Average
688,824
2,412,136
1,342,032
2,370,523
2,226,611
2,025,174
EA# 0111-13 Page 58 of 112
-------
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-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 10:41:33 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000 f.
500000
0
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Oct 22 14:30:55 2010
TIC: W1002873.D\data.ms
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:41:33 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
583
340
852
662
890
152
136
164
188
240
264
694978
2450968
1342042
2316172
2072984
1889069
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
243
964
872
624
858
136
311
423
112
99
82
172
176
330
212
244
90746
113905
110324
218740
187221
22208
242828
190827
4
5
5
5
5
4
5
4
79
17
10
27
08
19
03
97
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
At }
1-L 1
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
r^ TI T • j *-*-.— * T-I ' n ' T-I f~.
z, - IN i L. roani j. ine
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
•7
/
7
7
8
8
8
249
313
975
065
082
183
333
391
455
536
530
568
664
691
861
888
097
177
193
193
267
417
519
604
620
711
058
283
384
437
544
582
774
P A Q
o *± y
961
993
041
068
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
C [T
O D
168
163
168
165
nWi_
66491
106190
122087
53017
69681
104979
122495
124574
53085
118211
79720
88243
100657
68876
43746
105233
177985
67707m
51599
28941
112965
65593
105411
318534
32494
61216
61911
194818
189695
55760
55851
53497
186345
A r\ ~i c p
*± U Jj D o
22517m
204773
28240
46163
IQ^m*'
4
4
5
5
5
5
5
5
4
5
5
5
5
4
4
5
4
3
4
2
5
4
5
5
4
4
4
5
5
4
4
4
5
3
5
4
4
1 nfl-\
97
77
31
10
13
06
14
16
74
21
24
26
05
96
95
27
97
72
61
44
18
16
12
40
89
94
14
15
19
41
60
39
24
A r\
*± U
85
07
20
73
H
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
-0 . 02
-0 . 01
0 . 00
-0 . 01
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
/ T \\
ug/mijft
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
91
83
91
91
88
96
99
96
85
95
98
81
94
67
87
85
Manual Int. "II" (Et)E^|
92
1
96
95
98
93
77
98
89
100
97
100
98
90
95
[Manual Int. "WP" (EbE^
99
62
84
89
W101810X-8270.M Fri Oct 22 14:30:55 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:41:33 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
201
255
372
356
372
485
543
613
655
693
858
891
869
901
965
019
355
462
644
873
927
066
333
086
166
327
871
904
898
497
555
646
694
362
217
259
698
788
933
947
947
540
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
280459
43562
181422
10321
23474
52380
249463
42498
44942
201374
103250
197862
37898
19583
168974
182189
58744
61475
22599
279098
270861
231460
290388
275456
116352
306508
104995
138785
97568
136132
74279
255683
242579
196874
222593
223219
204034
202852
225687
207540m
170747
175572
5
4
5
2
3
4
5
4
4
5
4
5
4
3
5
5
4
4
3
5
5
5
5
5
4
5
4
4
4
4
4
5
5
3
4
4
4
4
4
5
4
5
20
54
26
28
45
32
30
16
32
14
98
12
15
03
01
19
84
88
37
34
19
10
01
06
15
25
58
31
77
33
85
10
15
97
71
85
85
66
99
92
71
01
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
98
74
94
100
81
92
95
97
75
100
95
100
81
17
95
100
98
94
92
98
99
98
97
98
92
99
100
100
100
100
95
98
99
100
99
94
99
98
QQ
Manual Int. "WP" (Et)E)|
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 64 of 112
W101810X-8270.M Fri Oct 22 14:30:55 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:24:37 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
0
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Oct 22 14:43:19 2010
TIC: W1002874.D\data.ms
.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:24:37 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
583
340
852
662
890
152
136
164
188
240
264
706693
2486145
1339110
2294140
2146192
1945018
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
238
964
872
625
859
136
311
423
112
99
82
172
176
330
212
244
189663
231671
219110
444619
366528
48270
497272
393732
9
10
9
10
9
9
10
10
84
34
96
74
97
19
40
34
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
^ — ' /
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
I sophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalat e
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
7
8
8
8
244
308
975
066
082
183
333
392
456
541
530
568
664
691
862
888
102
177
193
204
268
423
519
604
620
711
058
283
384
438
544
582
774
849
961
993
047
068
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
132455
223500
246658
107534
141015
215831
248947
254240
115259
239817
160017
175237
204756
142924
89308
209432
371720
144581m
109870
77311
227084
153620
212317
635629
67758
124967
135411
391569
376496
109877
119287
117762
370284
87885
51444m
413512
62670
95709
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:24:37 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
Q A \
y4 ;
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
-i /-
16
17
202
255
373
356
373
485
543
613
656
693
859
891
869
901
965
019
355
462
644
879
927
066
333
086
172
327
872
904
898
491
555
646
694
362
217
260
698
789
938
947
QC1
y bz
540
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
O ""7 O
2 la
276
565311
93155
351625
26144
53475
114585
485032
90233
93540
399969
204672
391149
79786
46162
342496
350420
120530
123010
53464
546524
532469
455826
614998
569316
221945
621484
233209
298947
213098
313443
144361
519832
500412
476626
470026m
478117
428593
429107
469953
409345m
Q A "i r\ ""? Q
3410 /3
343597
10
9
10
5
7
9
10
8
9
10
9
10
8
7
10
10
10
9
8
10
10
10
10
10
7
10
9
8
10
9
9
10
10
9
9
10
9
9
10
11
9
50
72
21
80
87
47
33
85
06
23
90
14
75
12
18
00
03
87
05
55
30
15
70
56
98
74
83
96
07
64
11
03
25
28
66
06
89
60
07
35
"1 A
14
52
ug/mL 98
ug/mL# 76
ug/mL 96
ug/mL# 100
ug/mL# 69
ug/mL 90
ug/mL 96
ug/mL 98
ug/mL# 78
ug/mL 99
ug/mL 96
ug/mL 99
ug/mL# 83
ug/mL# 12
ug/mL 95
ug/mL# 100
ug/mL 97
ug/mL 95
ug/mL 91
ug/mL 100
ug/mL 100
ug/mL 99
ug/mL 97
ug/mL 98
ug/mL 100
ug/mL# 99
ug/mL# 100
ug/mL# 100
ug/mL# 100
ug/mL# 99
ug/mL 100
ug/mL 98
ug/mL 99
ug/mL# 100
ug/mL
ug/mL 96
ug/mL 99
ug/mL 98
ug/mL 98
ug/mL
-1 1 /T / TVt T 44- Q /"""
ug/mL# 96
ug/mL 99
Manual Int. "WP" (Et)E^|
Manual Int. "WP" (Et)E)|
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 67 of 112
W101810X-8270.M Fri Oct 22 14:43:18 2010
Page: 2
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
(QT Reviewed)
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:28:56 2010
Response via : Initial Calibration
Abundance
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1002875.D\data.ms
§ I
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~
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o
o
o
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Oct 22 14:43:34 2010
140°
Page : 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:28:56 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 583
.340
. 852
. 662
.895
152
136
164
188
240
264
720068
2542711
1390154
2354525
2151499
2135523
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
. 964
. 872
. 624
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
385102
471390
454648
861131
734622
98664
938517
721401
19
20
20
20
19
18
19
18
.62
. 65
.28
. 03
.24
.30
.12
.47
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7 .
7 .
7 .
8.
8.
8 .
.249
.308
. 980
.066
.082
.183
.333
.391
.456
. 541
. 530
.568
.664
.691
.862
. 888
. 102
. 177
.193
.219
.268
.422
.519
. 609
. 620
. 711
. 058
.283
.384
.438
.544
. 582
. 774
. 849
. 966
. 998
.047
.073
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
268662
454960
488820
215533
286491
427306
487385
491902
236968
461266
322944
353103
412029
286292
177593
420879
727219
346769m
226482
218584
448403
298031
415380
1242897
135275
245812
292277
775011
755442
250781
242401
245683
732264
186464
111999m
808845
130890
191913
0 i-J 0 [T [T
•1Q ^S5^ RC
19
19
20
19
20
19
19
19
20
19
20
20
19
19
19
20
19
18
19
17
19
18
19
20
19
19
18
19
19
19
19
19
19
19
17
19
18
18
>^
.38
. 73
. 51
.99
.34
.87
.75
. 65
.41
. 60
.47
.30
.95
.90
.41
.36
.59
.90
.49
.74
.84
.38
.44
.30
. 62
. 13
. 84
.76
.92
.11
.27
.48
. 86
. 61
. 50
. 32
.77
.98
1^
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
93
82
92
91
89
95
99
99
84
94
96
82
96
71
87
86
en
Manual Int. "II" (EOE)|
92
1
97
95
98
89
77
99
90
99
99
100
98
94
94
84
jManual Int. "WP" (Et)E^|
76
87
89
W101810X-8270.M Fri Oct 22 14:43:33 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:28:56 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
201
255
372
356
372
485
543
607
656
693
859
891
875
901
971
019
355
468
644
879
927
066
333
086
172
327
872
904
898
497
555
646
694
362
217
260
703
794
938
952
952
545
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
1138687
187783
696007
75132
126824
237923
966279
191404
198571
796177
410164
788273
174390
115410
674512
731513
238946
241102
116269
1057464
1071540
893014
1194294
1065299
506627
1153486
471590
621800
427604
655163
296700
1018235
954008
1050503
981816
1016689
914623
942058
996589
1031781m
863105
870239
20
18
19
16
17
18
19
18
18
19
19
19
18
17
19
20
19
18
17
19
20
19
20
19
17
19
19
18
20
20
18
19
19
20
18
19
19
19
19
26
21
21
37
87
46
05
99
95
82
08
54
61
10
69
43
15
31
10
37
84
07
89
19
37
25
26
75
41
83
59
16
10
67
61
50
40
39
49
22
19
45
05
07
97
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
98
72
95
100
70
90
94
97
81
99
96
99
81
11
95
100
100
95
90
100
99
99
98
97
99
99
100
100
100
99
99
98
99
100
97
95
99
97
|Manual Int. "WP" (EDE^
96
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 70 of 112
W101810X-8270.M Fri Oct 22 14:43:33 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Abundance
1.2e+07i
TIC: W1002876.D\data.ms
1.156+07
1.16+07
1.056+07
1e+07
9500000
9000000
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
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W101810X-8270.M Fri Oct 22 14:43:50 2010
140°
Page : 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 588
.346
. 852
. 662
.895
152
136
164
188
240
264
655591
2346284
1314587
2336676
2279787
2168681
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
.969
. 872
. 625
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
900585
1027693
1032667
2022693
1817663
262774
2440734
1933841
50
49
50
49
50
49
50
49
.39
.44
. 60
. 76
.35
.12
.11
.88
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41 )
*± _L j
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2 -Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7
7 .
7.
8.
8.
8 .
.249
.308
. 980
.066
.087
.188
.333
.391
.461
. 541
. 536
.573
.669
.696
.862
. 894
. 107
. 177
. 198
.241
.273
.423
.519
. 610
. 620
. 711
. 058
.283
.384
.438
.544
. 582
. 779
84 Q
. O ^t ^1
. 966
.998
.052
.073
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
635596
1062640
1074210
494387
650353
979940
1116124
1134699
526916
1065553
705677
804523
924560
656842
419458
944597
1738085
895507
539145
634258
1042176
767304
963119
2807524
320545
574376
750985
1804232
1742830
638121
597498
598687
1729488
465188
301749m
1964791
339018
477324
_ic?^093 _
50
50
49
50
50
50
49
49
49
49
49
50
49
50
50
50
50
52
50
55
49
51
48
49
50
48
52
49
49
52
50
50
49
51
48
49
51
49
>£Pl
.35
. 61
. 50
.37
.72
.04
.68
.79
. 86
. 74
. 14
.80
.16
.14
.36
. 18
. 74
.91
.28
.80
.96
.28
.84
. 68
.38
.44
.45
.86
.79
.70
.23
.19
. 61
. 72
. 86
.64
.42
.91
r?5
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
UQ/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
91
82
91
89
89
96
98
99
85
95
97
82
94
72
87
87
96
61
93
1
97
96
98
86
75
99
91
99
99
100
99
90
94
0 C
iManual Int. "WP" (Et)E^|
99
71
72
89
W101810X-8270.M Fri Oct 22 14:43:49 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8.
8.
8 .
8 .
8 .
8 .
8.
8.
8.
8.
8 .
8 .
8 .
8 .
8.
9.
9.
9.
9.
9.
9.
10 .
10.
11.
11.
11.
11 .
11 .
11 .
12 .
12.
12.
12.
13.
14 .
14 .
14 .
14 .
14 .
16.
16 .
17.
.207
.260
.378
.356
.378
.490
.543
.613
.656
.698
. 859
. 896
. 880
. 907
.971
.019
.361
.468
. 644
. 879
. 932
. 066
.338
.092
.172
.332
. 877
. 904
. 898
.497
.561
.646
.700
.362
.222
.270
. 703
.799
. 943
.963
. 968
.556
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
2670061
479906
1669970
247053
363991
605293
2297357
508913
513321
1941442
999101
1910816
472667
345178
1651904
1784554
593204
615677
352570
2640876
2674012
2302237
3044317
2764843
1448854
2949812
1286236
1821139
1151877
1837456
779586
2743401
2601527
3014003
2638192
2627687
2368320
2483685
2553222
2666636m
2243251
2201757
50
51
49
55
54
50
49
50
50
50
49
50
52
54
50
51
48
48
52
50
50
50
52
50
51
50
51
51
51
53
46
49
50
55
48
49
49
49
49
66
53
54
.50
.01
.38
. 80
.59
.97
.83
.84
.67
.56
.21
.46
. 83
.25
.00
.85
.45
.49
. 15
. 06
. 77
.33
.01
.37
.15
.03
. 04
.38
.25
.19
.30
.85
.18
.25
. 65
. 60
. 02
. 83
. 07
.30
. 92
.74
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
97
72
94
100
70
90
94
97
81
99
96
99
85
12
96
100
99
94
90
99
100
99
99
98
99
98
100
100
100
100
99
99
99
100
98
95
99
97
i nn
iManual Int. "WP" (Et)E^|
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 73 of 112
W101810X-8270.M Fri Oct 22 14:43:49 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:32:38 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.7e+07
1.6e+07
1.5e+07
1.4e+07
1.3e+07
1.2e+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
Time~> 3.00
TIC: W1002877.D\data.ms
4.00
5.00
6.00
7.00
W101810X-8270.M Fri Oct 22 14:44:07 2010
^A#8^1--l^a^4^fl213-00 140° 15'°° 16'°° 17'°° 18'°°
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:32:38 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
852
667
895
152
136
164
188
240
264
662846
2259727
1246926
2303538
2250870
1934135
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
243
975
877
624
864
136
316
423
112
99
82
172
176
330
212
244
1520406
1676087
1643568
3079250
2847479
450730
4032039
3278436
84
79
79
79
83
85
83
85
13
75
65
87
16
46
98
79
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
8
249
302
985
071
087
188
333
391
461
541
536
573
674
701
861
893
107
182
198
257
273
422
518
609
625
711
063
282
389
437
544
582
779
854
972
004
057
078
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
1065824
1777926
1729656
797205
1046624
1618701
1841092
1864578
865190
1745698
1151734
1272195
1506894
1068651
693404
1499787
2678725
1483117
868327
1054266
1628238
1226169
1550822
4368870
501374
942468
1175601
2802443
2709029
979053
948267
952771
2680042
734512
484493m
3112785
546881
769244
,. ,3 7JJ4 4 7 7f
83
83
78
80
80
81
81
80
80
80
79
79
79
80
82
78
81
90
84
96
81
85
81
80
81
82
85
80
80
83
84
84
81
86
81
82
87
84
" Or/ f
51
75
83
33
73
75
06
93
97
59
32
45
24
68
34
80
20
99
08
30
05
09
66
28
81
53
26
41
37
96
04
20
04
10
29
91
44
80
W
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
90
83
92
88
91
95
99
99
86
95
97
82
95
71
89
86
96
79
91
1
97
96
99
86
75
99
93
100
99
100
99
89
94
Rfi
jManual Int. "WP" (EbE^
9 :?
68
74
88
W101810X-8270.M Fri Oct 22 14:44:06 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:32:38 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8.
8.
8 .
8 .
8 .
8 .
8.
8.
8.
8.
8 .
8 .
8 .
8 .
8.
9.
9.
9.
9.
9.
9.
10 .
10.
11.
11.
11.
11 .
11 .
11 .
12 .
12.
12.
12.
13.
14 .
14 .
14 .
14 .
14.
16.
16.
17.
.207
.260
.378
.362
.383
.490
.549
.613
.655
.698
. 858
. 896
. 885
. 912
.971
.024
.361
.467
. 644
. 879
. 932
. 071
.338
.091
.172
.337
. 877
.909
. 898
.496
.561
.651
.699
.362
.227
.270
. 708
. 804
.949
.968
.973
.561
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
4042909
756365
2583448
422751
590584
991694
3539646
841349
843608
3035443
1590412
2932322
759570
591276
2602966
2703666
978655
1022518
615106
4174914
4211925
3742990
4846729
4561098
2566248
4884640
2130889
3049279
1851064
2966387
1278774
4440131
4151696
4770907
4214544
3922979m
3629102
3812109
3851179
3625684m
3067089
2891515
80
84
80
100
92
88
80
88
87
83
82
81
89
97
83
82
81
81
92
80
81
83
84
84
91
84
85
87
83
86
76
81
81
88
87
83
84
85
82
101
82
80
.61
.76
. 54
. 67
.94
. 05
.95
.61
.79
.34
. 58
. 64
. 50
.97
.06
.82
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.69
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. 12
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.04
. 64
. 14
.42
.97
.92
.72
.11
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. 14
. 03
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. 76
.99
.07
.66
.60
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
96
74
94
100
72
88
93
98
82
98
97
100
85
10
96
100
98
92
89
99
99
99
99
97
99
97
100
100
100
99
98
98
100
100
llManual Int. "WP" (EbE^I
100
97
100
^jManual Int. "WP" (EDE)|
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 76 of 112
W101810X-8270.M Fri Oct 22 14:44:06 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:34:25 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abu
TIC: W1002878.D\data.ms
2.86+07
2.76+07
2.66+07
2.56+07
2.46+07
2.36+07
2.26+07
2.16+07
2e+07
1.96+07
1.86+07
1.76+07
1.66+07
1.56+07
1.46+07
1.36+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
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W101810X-8270.M Fri Oct 22 14:44:24 2010
140°
Page : 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:34:25 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
857
673
901
152
136
164
188
240
264
708059
2432537
1402728
2472354
2393551
2217223
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
#0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
248
975
878
624
869
142
321
423
112
99
82
172
176
330
212
244
2357516
2560855
2572034
4954156
4539684
763894
6105122
4961073
122
114
116
114
117
134
118
120
13
06
69
22
86
95
47
95
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2 -Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
8
254
308
991
071
092
188
333
391
466
541
536
573
680
707
862
899
118
193
198
278
278
428
519
609
625
711
064
288
389
443
550
587
779
854
977
009
063
084
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
1655479
2748558
2638757
1250650
1606548
2488942
2860266
2903770
1367289
2714894
1761446
1956537
2433885
1701666
1091581
2314661
4279898
2442858
1390088
1884010
2556990
2045970
2465876
6652351
810900
1526411
1993072
4510862
4367649
1697507
1575736
1597090
4326768
1185450
816936m
4936719
900830
1243711
1^585 _
121
121
112
117
116
117
117
117
119
117
113
114
119
120
121
113
120
139
125
159
118
131
120
113
122
124
134
120
120
135
124
125
116
123
119
116
128
121
pl2,4.
42
20
58
97
01
68
89
98
78
33
57
38
82
26
34
84
52
42
04
86
23
90
61
55
92
17
27
24
36
23
13
47
31
53
66
89
04
87
W
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ucr/mL
|_L^-J / LLLJ-J
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
85
90
95
99
99
86
95
97
81
95
71
91
89
95
95
92
1
97
96
98
82
73
99
91
100
99
100
100
89
93
H-7
Manual Int. "WP" (EbE^
72
76
88
W101810X-8270.M Fri Oct 22 14:44:24 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:34:25 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
212
271
383
367
388
495
549
618
661
704
864
901
891
917
976
024
361
473
649
884
938
071
338
097
177
343
877
909
898
497
566
657
710
367
238
281
719
815
959
989
984
577
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
6412169
1247776
4267963
758110
1003648
1578371
5664751
1400720
1402198
4716072
2628420
4706583
1227582
987659
4133249
4180139
1612330
1701383
1052185
6387152
6488275
5721649
7022165
6782097
4250745
7136986
3186427
4879382
2743784
4377746
2373255
6897657
6402828
7148627
6718692
6627053
6027853
6326927
6400912
6045974m
5197283
4731566
113
124
118
160
140
124
115
131
129
115
121
116
128
145
117
113
124
126
147
114
116
118
113
116
141
114
120
131
116
120
134
119
117
124
121
122
122
124
120
147
122
115
65
30
28
48
40
57
15
14
71
11
32
49
57
47
24
83
47
64
10
44
42
21
39
78
82
40
43
13
28
70
24
38
64
81
18
38
03
14
33
02
19
06
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
95
76
92
100
74
87
92
98
82
97
98
99
88
10
97
100
97
90
89
97
98
99
99
96
98
96
100
100
100
98
99
97
99
100
98
94
100
96
3Q
[Manual Int. "WP" (EbE^
/
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 79 of 112
W101810X-8270.M Fri Oct 22 14:44:24 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
Abundance
3.4e+07
3.2e+07
3e+07
2.8e+07
2.6e+07
2.4e+07
2.26+07
2e+07
1.86+07
1.66+07
1.46+07
1.26+07
1e+07
8000000
6000000
4000000
2000000
0
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Oct 22 14:44:53 2010
TIC: W1002879.D\data.ms
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
858
673
901
152
136
164
188
240
264
673532
2366580
1358679
2516253
2291395
1886569
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
# 0.
0 .
0 .
0 .
0 .
0.
00
00
00
00
00
00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
248
980
883
630
869
142
321
428
112
99
82
172
176
330
212
244
2937218
3293639
3320235
6215938
5946373
1063166
8046097
6485723
159
154
158
147
159
184
153
155
96
22
36
96
38
54
41
36
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
255
308
991
076
092
194
333
391
472
541
541
573
680
712
867
899
118
198
203
294
278
428
524
609
625
711
064
288
389
443
550
587
779
860
977
014
068
089
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
2078189
3461744
3371914
1566019
1996432
3120918
3568480
3597095
1759118
3382190
2305638
2460461
3125251
2127745
1364287
2965421
5347160
3051656
1796992
2443160
3186218
2620544
3137769
8215827
1008461
1961876
2554086
5580915
5353707
2101657
2051735
2123898
5435141
1548645
1091043m
64896 98
1200737
1659543
160
160
151
155
151
155
154
153
162
153
156
151
161
158
159
153
154
179
166
213
151
173
157
144
157
164
176
152
151
172
166
172
150
166
162
158
176
167
24
48
24
29
55
12
62
65
01
67
25
22
74
08
43
33
77
01
15
08
43
64
75
15
13
04
83
90
65
10
87
27
84
60
60
64
20
89
8 ' E=^ oWl - 1 3P ^aqe 8 1 Wl tt
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
82
91
95
99
99
86
94
9
6
80
95
71
93
88
94
97
93
1
97
96
98
81
75
99
93
99
100
100
99
90
92
87
9
(Manual Int. "WP" (EbE^
o
75
78
8
8
W101810X-8270.M Fri Oct 22 14:44:52 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
212
271
383
367
394
501
554
618
661
709
864
901
896
923
981
030
361
473
649
884
938
071
338
097
182
343
882
914
904
497
571
657
710
367
238
286
719
815
959
984
989
577
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
8115474
1651596
5451356
1061235
1347780
2126518
7226840
1923007
1852921
6077894
3463398
6120247
1667054
1373367
5463024
5335363
2194998
2292317
1449126
8396637
8383752
7617280
8883374
8829464
5525908
9167949
4110291
6289525
3588752
5599108
3194173
8682401
8108075
8822468
8302487
7385841
6925339
7257690
7263792
6422568m
5561636
4959425
148
169
155
231
194
173
151
185
176
153
165
156
180
208
159
149
166
167
199
147
147
154
140
149
181
144
162
176
158
161
188
156
155
160
175
160
164
167
160
183
153
141
51
86
98
92
65
27
67
88
96
16
04
39
27
84
98
99
50
65
06
82
81
63
95
38
15
39
28
56
87
26
73
97
61
90
99
30
78
35
48
55
67
74
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
94
76
93
100
74
87
91
98
81
96
99
99
91
8
98
100
96
90
89
96
96
97
98
95
98
94
100
100
100
96
98
96
97
100
98
93
100
96
3 a
[Manual Int. "WP" (EbE^
/
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 82 of 112
W101810X-8270.M Fri Oct 22 14:44:52 2010
Page: 2
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Min.
Max.
1 I
2
3
4 S
5 S
6
7
8
9
0
1
2
3
4
5
6
7
8
9 S
0
1 I
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6 I
7
8
9 S
0
1
2
3
4
5
6
7
8
9
0
1
2
RRF : 0.050 Min. Rel. Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
1 , 4-Dichlorobenzene-d4 (I)
N-Nitrosodimethylamine
Pyridine
2-Fluorophenol (surr)
Phenol -d5 (surr)
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) ethe
3/4 -Methylphenol
N-Nitrso-di-n-propylamine (
Hexachloroe thane
Nitrobenzene-d5 (surr)
Nitrobenzene
Naphthalene-dS (I)
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2 -Chloroethoxy) methane
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylphenol (CC
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadiene (
Acenaphthene-dlO (I)
2, 4, 6-Trichlorophenol (CCC)
2,4, 5-Trichlorophenol
2-Fluorobiphenyl (surr)
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2 , 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
,=9,
. 000
. 770
.281
.091
.268
.324
.599
. 782
.195
.371
.390
.645
.307
.876
. 966
. 148
.799
. 508
.245
.149
.000
. 584
.288
. 183
. 194
.356
.255
.336
.963
. 108
.202
.244
. 617
.597
.206
.000
.362
.363
.237
. 061
.274
.194
.204
.201
.291
. 136
. 609
.286
.029
.135
.204
^Wn
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
0
0
0
* Oi
. 000
. 756
.260
.096
.246
.264
. 579
. 760
. 165
.319
.349
.639
.246
.830
. 942
. 097
. 773
.496
.221
.107
.000
. 587
.310
. 182
.218
.352
.258
.317
.944
. 109
. 196
.253
. 575
.587
.207
.000
.355
.366
.213
. 040
.277
.179
.182
.202
.289
. 136
. 602
.282
.994
.144
.208
o3«_,
0 .
1 .
1.
-0.
1.
4.
3 .
2 .
2 .
3 .
2.
0.
4.
5.
2 .
4 .
3 .
2 .
1.
3.
0.
-0 .
-7 .
0 .
-12 .
1.
-1.
5.
2.
-0 .
3 .
-3 .
6 .
1.
-0.
0.
1 .
-0 .
1 .
2 .
-1.
7.
1.
-0.
0 .
0 .
0 .
1 .
3.
-6.
-2.
-» Ql'JJf
. 0
. 8
.6
.5
.7
.5
.3
. 8
. 5
. 8
.9
.9
.7
.3
. 5
.4
.3
.4
.9
.7
.0
. 5
. 6
. 5
.4
.1
.2
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.0
.9
. 0
. 7
. 8
.7
.5
.0
.9
. 8
.9
. 0
.1
.7
.8
.5
. 7
. 0
.4
.4
.4
.7
.0
•1*
105
102
102
105
104
101
101
100
102
102
102
104
101
101
101
102
101
102
102
101
102
101
104
102
103
101
101
99
101
102
102
101
96
101
98
102
100
103
101
101
100
100
101
100
102
102
101
99
100
98
96
0101
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
W101810X-8270.M Mon Oct 25 09:59:33 2010
Page: 1
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Min.
Max.
53
54
55
56
57 S
58
59
60
61
62
63
64 I
65 S
66
67
68
69
70
71
72
73
74
75 S
76
77 S
78 I
79
80
81
82
83
84
85
86
87 I
88
89
90
91
92
93
94
95
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
Fluorene-dlO (surr)
4-Chlorophenyl-phenylether
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine (CCC
Azobenzene
Phenanthrene-dlO (I)
2 , 4 , 6-Tribromophenol (surr)
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene-dlO (surr)
Pyrene
Terphenyl-dl4 (surr)
Chrysene-dl2 (I)
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phthalate
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Perylene-dl2 (I)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
1
0
0
1
1
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
0
0
0
1
0
1
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
.403
.305
.308
.168
.098
.618
. 152
.272
. 194
. 005
.047
.000
.092
.210
.217
. 116
. 903
.902
.783
.002
.940
.485
. 834
. 009
. 664
.000
.442
.622
.394
. 606
.295
. 966
.910
.957
.000
. 000
. 977
. 891
. 920
.960
.794
. 767
.742
1
0
0
1
0
0
1
0
0
0
1
1
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
0
0
1
1
1
0
0
0
0
0
0
0
.338
.304
.308
.145
.983
.594
. 105
.272
.200
. 983
.020
.000
.097
.207
.215
. 122
. 885
.899
.792
.013
.907
.438
. 890
. 970
. 654
.000
.477
.625
.423
. 664
.294
. 956
.894
.048
.000
. 014
. 940
.916
.911
.928
.901
. 757
.738
4 .
0 .
0.
2.
10.
3.
4 .
0 .
-3 .
2 .
2.
0.
-5.
1 .
0 .
-5 .
2 .
0.
-1.
-1.
3.
9.
-6 .
3 .
1 .
0.
-7.
-0.
-7 .
-9.
0 .
1 .
1.
-9.
0.
-1 .
3 .
-2 .
1 .
3.
-13.
1.
0.
. 6
.3
.0
.0
.5
.9
. 1
. 0
. 1
.2
.6
.0
.4
.4
.9
.2
. 0
.3
.1
.1
.5
. 7
. 7
.9
. 5
.0
.9
.5
.4
. 6
.3
. 0
.8
.5
.0
.4
. 8
. 8
. 0
.3
.5
.3
.5
98
100
101
99
91
100
97
97
98
100
96
99
107
101
101
101
97
98
100
97
95
88
106
95
98
88
94
87
93
91
95
88
87
88
82
85
79
86
81
80
75
75
74
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
-0.
0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.01
.00
(#) = Out of Range
SPCC's out = 0 CCC's out = 0
EA# 0111-13 Page 84 of 112
W101810X-8270.M Mon Oct 25 09:59:33 2010
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
8270C - Full List
Tue Oct 19 10:23:46 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.15e+07
1.1e+07
1.056+07
500000
Time->300
TIC: W1002880.D\data.ms
4.00
5.00 6.00 7.00 8.0*^ flfl ^
W101810X-8270 .M Mon Oct 25 09:59:17 2010
13.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 588
.340
. 852
. 662
.890
152
136
164
188
240
264
688226
2399400
1346787
2321939
2016217
1770087
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
-0 . 01
-0.01
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
.969
. 872
. 624
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
942676
1072314
1050722
2042041
1654743
282026
2584320
1897215
50
49
49
49
44
53
53
49
.24
. 14
. 04
. 04
. 74
.05
.40
.25
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7 .
7 .
7.
8.
8.
8 .
.249
.308
. 980
.065
.087
.188
.333
.391
.461
. 541
. 530
.573
.669
.696
.861
. 894
. 107
. 177
. 198
.241
.273
.422
.519
. 609
. 620
. 711
. 058
.282
.384
.437
.544
. 582
. 774
. 849
. 966
.998
.052
.073
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
nWi_
650462
1084039
1087452
497934
653516
1002204
1134739
1160863
550127
1071979
713985
810431
943342
665016
427064
952529
1759588
930823
547329
653741
1054912
773012
949915
2831328
326892
588090
757761
1723519
1760290
622246
598454
616336
1750104
466776
301474
1989983
339326
486482
99 QT 9 q
.•nr45o«£ «
49
49
47
48
48
48
48
48
49
47
47
48
47
48
48
48
50
52
49
56
49
50
47
49
50
48
51
46
49
50
49
50
49
50
51
49
50
49
R /vf9-!
. 08
. 18
. 73
.32
.55
.75
.12
. 53
. 58
. 66
.36
.75
.78
.35
.84
.20
.23
.23
.91
.24
.45
.07
.10
. 00
.24
. 50
. 75
.57
.18
.26
.12
.43
. 00
. 66
.31
.07
.23
.65
r?3
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
-0 . 01
-0 . 01
0 . 00
0 . 00
0.00
-0.01
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
90
89
95
99
99
88
95
96
82
95
71
88
87
96
67
90
1
97
96
97
87
73
99
93
100
99
100
99
92
94
86
85
99
77
75
88
W101810X-8270.M Mon Oct 25 09:59:16 2010
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2,3 -cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
207
260
378
356
378
484
543
613
655
698
858
896
880
907
971
019
355
467
644
879
927
066
333
086
172
332
871
904
898
491
555
646
694
362
222
265
703
794
938
957
957
550
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
2697323
475207
1672802
243039
349836
609727
2253212
511289
518904
1926868
1000592
1860174
457380
336568
1654790
1717854
601962
623183
354702
2568324
2608767
2298332
2940310
2633790
1271135
2814190
1202690
1576400
1066265
1673071
741563
2409352
2253864
2642075
2244455
2079980
2027457
2014918
2052550
1993996m
1674843
1633341
49
49
48
53
51
50
47
49
49
48
48
47
49
51
48
48
49
49
52
49
49
50
50
48
45
48
53
50
53
54
49
49
49
54
50
48
51
49
48
60
49
49
80
30
29
58
21
12
71
86
75
98
10
95
90
84
89
72
48
39
80
01
84
56
57
29
17
06
98
30
63
74
83
40
18
74
68
24
39
42
42
71
32
73
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
97
72
94
100
71
89
94
97
81
99
97
100
86
11
95
100
97
92
90
99
100
99
99
98
99
98
100
100
100
100
99
98
99
100
98
94
99
97
"[Manual Int. "WP" (EOE)|
96
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 87 of 112
W101810X-8270.M Mon Oct 25 09:59:16 2010
Page: 2
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100042.D
28 Jan 2011 12:57 pm
tdd
sstdOSO M8270 Std
2 Sample Multiplier: 1
Quant Time Jan 28 13:26:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Min.
Max.
1 I
2
3
4 S
5 S
6
7
8
9
0
1
2
3
4
5
6
7
8
9 S
0
1 I
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6 I
7
8
9 S
0
1
2
3
4
5
6
7
8
9
0
1
2
RRF : 0.050 Min. Rel. Area : 50% Max.
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF
1 , 4-Dichlorobenzene-d4 (I)
N-Nitrosodimethylamine
Pyridine
2-Fluorophenol (surr)
Phenol -d5 (surr)
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) ethe
3/4 -Methylphenol
N-Nitrso-di-n-propylamine (
Hexachloroe thane
Nitrobenzene-d5 (surr)
Nitrobenzene
Naphthalene-dS (I)
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2 -Chloroethoxy) methane
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylphenol (CC
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadiene (
Acenaphthene-dlO (I)
2, 4, 6-Trichlorophenol (CCC)
2,4, 5-Trichlorophenol
2-Fluorobiphenyl (surr)
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2 , 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
. 000
. 770
.281
.091
.268
.324
.599
. 782
.195
.371
.390
.645
.307
.876
. 966
. 148
.799
. 508
.245
.149
.000
. 584
.288
. 183
. 194
.356
.255
.336
.963
. 108
.202
.244
. 617
.597
.206
.000
.362
.363
.237
. 061
.274
.175
.204
.201
.291
. 136
. 609
.286
.029
.135
.204
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
. 000
. 672
.219
.040
.250
.275
. 571
. 759
. 172
.350
.389
.646
.338
.901
. 908
. 104
. 733
. 516
.242
.098
.000
. 550
.251
. 187
. 183
.320
.246
.329
.993
. 102
.201
.234
. 617
.599
.210
.000
.355
.334
.253
. 061
.262
.172
.195
.196
.286
. 129
. 659
.251
.068
.139
.186
R.T. Dev O.SOmin
%Dev Area% Dev(min)
0 .
12 .
4.
4.
1.
3.
4 .
2 .
1 .
1 .
0.
-0.
-2.
-2.
6 .
3 .
8 .
-1 .
0.
4.
0.
5 .
12 .
-2 .
5 .
10.
3.
2.
-3.
5 .
0 .
4 .
0 .
-0.
-1.
0.
1 .
8 .
-1 .
0 .
4.
1.
0.
2.
1 .
5 .
-3 .
12 .
-3.
-3.
8.
. 0
. 7
.8
.7
.4
.7
. 7
.9
.9
. 5
.1
.2
.4
.9
. 0
. 8
.3
. 6
.2
.4
.0
. 8
. 8
.2
. 7
.1
.5
.1
.1
. 6
. 5
. 1
. 0
.3
.9
.0
.9
. 0
.3
. 0
.4
.7
.7
.5
. 7
. 1
. 1
.2
.8
.0
.8
EM 61! 1 1 -^ S3 ^aqe 88 of f 1 2
86
74
81
81
86
84
81
82
84
85
86
86
88
90
79
84
79
87
85
82
84
78
69
85
71
76
79
84
87
79
86
77
84
85
81
82
80
75
84
83
76
77
82
78
81
77
84
71
86
76
69
81
0 .
-0 .
-0.
-0.
-0.
-0.
-0 .
-0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
-0 .
-0 .
-0 .
-0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
-0.
0.
0.
-0.
0 .
-0 .
0 .
0 .
0.
0.
0.
0.
. 00
. 03
.03
.02
.01
.01
. 01
. 01
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 02
. 02
. 01
. 05
.00
.00
.00
.00
. 00
. 00
. 01
. 00
.01
.01
.00
. 00
. 00
. 00
. 00
.01
.00
.00
.01
. 00
. 01
. 00
. 00
.00
.00
.00
.00
W101810X-8270.M Fri Jan 28 18:49:18 2011
Page: 1
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100042.D
28 Jan 2011 12:57 pm
tdd
sstdOSO M8270 Std
2 Sample Multiplier: 1
Quant Time Jan 28 13:26:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Min.
Max.
53
54
55
56
57 S
58
59
60
61
62
63
64 I
65 S
66
67
68
69
70
71
72
73
74
75 S
76
77 S
78 I
79
80
81
82
83
84
85
86
87 I
88
89
90
91
92
93
94
95
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
Fluorene-dlO (surr)
4-Chlorophenyl-phenylether
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine (CCC
Azobenzene
Phenanthrene-dlO (I)
2 , 4 , 6-Tribromophenol (surr)
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene-dlO (surr)
Pyrene
Terphenyl-dl4 (surr)
Chrysene-dl2 (I)
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phthalate
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Perylene-dl2 (I)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
1
0
0
1
1
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
0
0
0
1
0
1
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
.403
.305
.308
.168
.098
.618
. 152
.272
. 194
. 005
.047
.000
.092
.210
.217
. 116
. 903
.902
.783
.002
.940
.485
. 834
. 009
. 664
.000
.442
.622
.394
. 606
.295
. 966
.910
.957
.000
. 000
. 977
. 891
. 920
.960
.910
. 767
.742
1
0
0
1
1
0
1
0
0
0
1
1
0
0
0
0
0
0
0
1
1
0
0
1
0
1
0
0
0
0
0
0
0
1
1
1
1
0
0
0
0
0
0
.425
.307
.304
.221
.179
.643
. 197
.246
.217
.998
.048
.000
.091
.210
.216
.119
. 945
.940
.754
.137
.011
.358
.909
. 122
. 718
.000
.504
.555
.440
. 695
.212
. 958
.897
.133
.000
. 067
. 013
. 926
.959
.963
.892
.748
.697
-1 .
-0 .
1.
-4.
-7.
-4.
-3 .
9.
-11 .
0 .
-0.
0.
1.
0 .
0 .
-2 .
-4 .
-4.
3.
-13.
-7.
26 .
-9.
-11 .
-8 .
0.
-14.
10.
-11 .
-14 .
28 .
0 .
1.
-18.
0.
-6 .
-3 .
-3 .
-4 .
-0.
2.
2.
6.
. 6
. 7
.3
.5
.4
.0
.9
. 6
.9
. 7
.1
.0
.1
. 0
. 5
. 6
. 7
.2
.7
.5
.6
.2#
. 0
.2
. 1
.0
.0
.8
. 7
. 7
.!#
. 8
.4
.4
.0
. 7
. 7
.9
.2
.3
.0
.5
.1
84
82
80
85
88
87
85
70
85
82
79
82
83
85
85
81
86
85
79
90
88
59
90
92
89
85
95
74
93
92
66
85
83
91
73
80
76
77
76
75
66
66
63
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
-0.
0.
-0.
-0 .
-0 .
-0 .
-0 .
-0.
-0.
-0.
-0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 01
.00
.00
.00
. 00
. 00
. 00
. 00
.01
.00
.01
. 01
. 02
. 02
. 02
.02
.04
.05
.06
(#) = Out of Range
SPCC's out = 0 CCC's out = 0
EA# 0111-13 Page 89 of 112
W101810X-8270.M Fri Jan 28 18:49:18 2011
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100042.D
28 Jan 2011 12:57 pm
tdd
sstdOBO M8270 Std
2 Sample Multiplier: 1
Quant Time Jan 28 13:26:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Jan 04 17:58:34 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.1e+07
1.05e+07
1e+07
9500000
9000000
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1100042.D\data.ms
>.
Time-> 3.00 4.00 5.00 6.00 7.00
W101810X-8270.M Fri Jan 28 18:49:07 2011
3.00 14.00 15.00 16.00 17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100042.D
28 Jan 2011 12:57 pm
tdd
sstdOSO M8270 Std
2 Sample Multiplier: 1
Quant Time Jan 28 13:26:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
290
503
260
772
566
751
152
136
164
188
240
264
563777
1972475
1079691
1925531
1937140
1582057
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
-0.01
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
157
889
792
544
778
056
230
343
112
99
82
172
176
330
212
244
733218
880550
875414
1690743
1590688
218199
2187302
1728860
47
49
49
50
53
49
54
54
70
26
88
65
65
49
50
12
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
•-» A \
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2 -Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
4
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
7
7
8
169
222
900
985
001
103
247
306
375
455
450
488
589
616
776
808
022
102
113
161
193
342
433
524
540
625
983
197
304
357
464
502
694
769
886
924
972
998
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
0 \i 1 -
473442
859015
898482
402191
535064
826138
951419
978962
455397
942868
634770
639561
778064
516773
363894
773521
1355248
619019
460894
449985m
789198
607162
811004
2447550
252327
494375
576031
1520281
1477407
517427
479358
451111
1431810
353341
231691
1613246
263871
386556
IS^aqeQI
43
47
48
47
48
49
49
49
50
51
51
46
48
45
50
47
47
43
51
47
45
48
48
51
47
49
47
49
50
50
49
46
50
47
49
49
48
49
of71
61
57
14
65
52
06
25
96
11
18
38
96
11
87
80
78
06
57
13
09
00
26
92
52
17
60
85
97
21
84
06
04
00
83
18
62
72
21
ft
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
-0.02
-0 . 01
0 . 00
0 . 00
0 . 00
0.00
0.00
0.01
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
93
81
89
87
94
96
100
99
85
95
97
74
94
76
88
87
94
89
9 ^Manual Int. "II" (TOC^
99
96
96
91
80
98
93
100
99
100
98
85
93
86
87
99
74
66
90
W101810X-8270.M Fri Jan 28 18:49:07 2011
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100042.D
28 Jan 2011 12:57 pm
tdd
sstdOSO M8270 Std
2 Sample Multiplier: 1
Quant Time Jan 28 13:26:07 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Jan 04 17:58:34 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
121
180
292
282
303
410
463
533
575
618
778
810
800
826
891
939
281
382
564
793
847
986
258
006
092
252
797
823
818
406
464
550
598
255
094
137
564
660
799
781
781
358
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
2239230
338471
1440750
188016
251478
493308
1922916
414923
410576
1648045
867904
1615698
332051
293246
1346400
1414821
505116
520785
285738
2273544
2263562
1815118
2735969
2432995
860835
2701125
1219243
1344289
1066599
1683290
513035
2320211
2172223
2743655
2110628
2002353m
1830304
1896855
1903897
1763578
1478831
1377411
51
43
51
51
45
50
50
50
49
52
52
51
45
56
49
50
50
49
51
52
52
48
56
53
36
55
56
44
55
57
35
49
49
59
53
51
51
52
50
49
48
46
56
80
88
71
70
58
79
47
34
26
05
95
18
12
62
05
07
77
29
30
15
15
73
79
88
59
94
64
85
35
86
62
31
19
35
82
93
14
16
01
72
94
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
/ _
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
98
76
94
100
67
94
91
98
79
100
94
100
78
15
96
100
98
96
88
99
100
99
99
99
99
98
100
100
100
98
98
98
99
100
QR
iManual Int. "WP" (TOC^
98
99
95
98
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
EA# 0111-13 Page 92 of 112
W101810X-8270.M Fri Jan 28 18:49:07 2011
Page: 2
-------
DFTPP
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Integration File: rteint.p
Method : M:\ms2010\methods\wiley\W101810X-8270.M
Title : 8270C - Full List
Last Update : Wed Oct 20 14:58:44 2010
Abundance
4000000
3000000
2000000
1000000
0
Time~>
Abundance
300000
250000
200000
150000
100000
50000
n
8.20
TIC: W1002872.D\data.ms
8.40
8.60 8.80
77.1
51
.1
i ii ,
III
107
.1
9.00 9.20 9.40 9.6C
Average of 10.0
19
127
M
148.! 167.1^
— I — I — I — I — 1 —
) 9.80
23 to 1 0
3.0
22
A . II 11.359 11.J01 I
i — i — i — f — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — \ — i — r • r i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i
10.00 10.20 10.40 10.60 10.80 11.00 11.20 11.40 11.60 11.80
034 min.: W1002872.D\data.ms (-)
442.1
255.1
275.1
4.1
I'lii.i.
296.1
..„ |, , ,„ , , 323-1, 346.0 365-°383.1 403.1 423/1
m/z~>
40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440
AutoFind: Scans 1318, 1319, 1320; Background Corrected with Scan 1312
Target
Mass
51
68
69
70
127
197
198
199
275
365
441
442
443
Rel. to
Mass
198
69
198
69
198
198
198
198
198
198
443
198
442
Lower
Limit%
30
0 . 00
0 . 00
0 . 00
40
0.00
100
5
10
1
0 . 01
40
17
Upper
Limit%
60
2
100
2
60
1
100
9
30
100
100
100
23
Rel.
Abn%
30.0
0 . 0
38 . 9
0 . 6
47 .2
0.0
100.0
7.1
27.9
4 . 1
77 . 9
77 . 1
19.1
Raw
Abn
92083
0
119435
751
144776
0
306901
21658
85621
12456
35283
236523
45283
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
EA# 0111-13 Page 93 of 112
W101810X-8270.M Mon Oct 25 10:18:03 2010
Page: 1
-------
Average of 10.023 to
DFTPP
Modified:subtracted
10.034 min.: W1002872.D\data.ms
m/z
35 . 10
36 . 05
37 . 00
38 . 10
39.15
40.10
41.15
45.00
45 .20
48 . 10
49 . 10
Average of
DFTPP
abund .
71
140
389
1201
7406
153
124
193
44
47
588
10.023 to
m/z
50 . 10
51 . 10
52 . 15
53 . 10
55.10
56.10
57.10
58.10
58 . 90
60 . 00
61 . 10
10.034 min.
abund .
25623
92083
4613
249
668
3048
6631
250
78
124
1482
m/z
62 . 10
63 . 10
64 . 05
65 . 10
65.80
66.15
67.10
69.10
70 . 10
71 . 10
72 . 00
. : W1002872 .D\data.
abund .
1589
4232
599
2029
55
120
204
119435
751
58
114
. ms
m/z
73 . 10
74 . 10
75 . 10
76 . 15
77.10
78.10
79.10
80.10
81 . 10
82 . 10
83 . 10
Modified : subtracted
m/z
83.85
84 . 10
84 .30
85 . 10
86 . 10
87.05
88.05
89.00
89.90
90 .30
91 . 10
Average of
DFTPP
abund .
99
126
116
1840
2620
1311
367
278
35
47
2159
10.023 to
m/z
92.10
93 . 10
94 . 05
95 . 05
96 . 15
98.10
99.10
100.10
101.05
101 . 90
103 . 10
10.034 min.
abund .
2468
15303
1071
347
586
11724
9039
873
4787
341
1964
m/z
104.10
105 . 10
106 . 00
107 . 10
108 . 10
110.10
111.10
112.10
113.00
113 . 15
114 . 80
. : W1002872 .D\data.
abund .
3541
2975
177
39725
5856
70621
11083
1404
177
216
78
. ms
m/z
115.00
116 . 10
117 . 10
118 . 10
119 . 10
120.05
121.00
122.05
123.10
124 . 10
125 . 10
Modified : subtracted
m/z
127.10
128.10
129 . 10
130 . 05
131 . 05
132 . 00
133.05
134.05
135.10
136.10
137 . 10
Average of
DFTPP
abund .
144776
11349
58907
4828
930
396
394
1606
4589
1650
2650
10.023 to
m/z
137.80
138.20
139 . 00
140 . 00
141 . 10
142 . 00
143.05
144.15
145.00
146.05
147 . 05
10.034 min.
abund .
464
83
289
732
7532
2457
1791
470
328
1588
3771
m/z
148.10
149.10
150 . 05
151 . 10
151 . 90
152 .20
153.00
154.10
155.10
156.10
157 . 05
. : W1002872 .D\data.
abund .
8660
1984
661
997
313
399
2621
1608
4353
6416
1396
. ms
m/z
158.05
159.05
160 . 05
161 . 10
162 . 00
163 . 10
164.10
165.05
166.10
167.10
168 . 05
Modified : subtracted
m/z
169.10
170.05
170.40
171 . 00
172 . 10
173 . 10
174 . 05
175.10
176.10
177.00
178.05
Average of
DFTPP
abund .
1368
566
216
688
1354
2000
3320
5899
1852
3035
753
10.023 to
m/z
179.00
180.10
181.10
182 . 00
182 . 90
184 . 05
185 . 10
186.10
187.05
188.05
189.05
10.034 min.
abund .
11587
7881
3852
592
349
828
5521
43536
12341
1310
2902
m/z
190.10
191.05
192.10
193 . 10
194 . 00
195 . 00
196 . 10
198.00
199.00
200.00
201.55
. : W1002872 .D\data.
abund .
527
1274
3773
4234
954
402
10417
306901
21658
1542
1992
. ms
m/z
203.05
204.10
205.10
206 . 10
207 . 10
208 . 05
209 . 05
210.05
211.10
211.90
213.05
Modified : subtracted
m/z
214.20
215.00
216.10
217.00
218 . 00
219 .20
221 . 10
221 . 90
223.00
224.10
225.10
Average of
abund .
58
938
1914
22667
2506
250
18122
1255
5264
48035
11551
10.023 to
m/z
226.10
227.05
228.00
229.05
229 . 95
231 . 10
231 . 90
232 . 10
233.00
233.20
234.05
10.034 min.
abund .
1311
20096
2934
4092
612
1744
74
288
246
173
1356
. : W100.2J
m/z
235.10
236.10
237.10
237.90
238 . 10
239 . 00
240 . 05
241 . 00
242.05
243.10
244.10
i72 ^DXd^ta,.
abund .
1361
924
1915
146
116
965
723
1150
2715
2626
36661
m/z
245.10
246.00
247.05
248.05
249 . 00
250 . 00
251 . 05
252 . 10
253.10
255.10
256.10
—r A A o
abund.
1095
12303
18631
6251
123147
8219
8921
7354
9291
2334
1632
abund.
47
1081
33520
2531
243
477
228
3061
4126
2074
1728
abund.
1390
1023
2349
3680
1057
254
412
2639
2444
15701
7359
abund.
2362
11774
20593
83491
11545
2714
934
806
3361
95
233
abund.
4908
7769
1547
339
1384
235
299
358
876
190088
27339
W101810X-8270.M Mon Oct 25 10:18:03 2010
Page: 2
-------
DFTPP
Modified:subtracted
m/z
257.10
258 . 00
259 . 00
260 . 00
260 . 95
262.20
262.90
264.05
265.00
265 . 90
267 . 00
abund .
2400
11841
2008
312
301
50
120
363
4967
720
247
Average of 10.023 to
DFTPP
Modified:
m/z
302.00
303.10
304 . 05
304 . 90
305 . 15
306 . 00
306.95
308.05
308.70
309.00
309 .20
subtracted
abund .
466
2920
898
75
99
48
100
391
39
254
58
Average of 10.023 to
DFTPP
Modified:
m/z
343.00
343.30
346.00
347 . 05
350 . 10
351 . 15
352 . 05
353.00
354.10
355.00
358.00
subtracted
abund .
55
43
1783
370
123
93
2544
1802
2480
455
50
Average of 10.023 to
DFTPP
Modified:
m/z
415.05
416.90
420.00
421.05
422 . 10
423 . 10
424 . 05
425 . 05
426.10
427.10
431.70
subtracted
abund .
141
38
95
1802
1923
12408
3115
396
39
53
60
m/z
267.90
268 . 90
269 . 70
269 . 95
271 . 00
272.00
273.10
274.05
275.10
276 . 05
277 . 05
10.034 min.
m/z
310.05
311.10
312 . 00
312 .20
312 . 90
313 . 05
314.00
315.05
316.10
317.10
320 . 00
10.034 min.
m/z
358.90
359.15
360.80
363 . 70
365 . 00
366 . 05
367 . 10
370.05
371.10
372.05
373.10
10.034 min.
m/z
434.10
434.40
436.80
437.30
437 . 80
438 .45
439 .20
439 . 70
441.10
442.10
443.10
abund .
132
37
68
225
445
304
5792
15820
85621
11521
7677
m/z
278.05
279 . 05
281 . 00
282 . 10
283 . 05
284.05
285.10
286.00
288.10
288 . 95
290 . 05
. : W1002872 .D\data.
abund .
370
49
47
42
81
244
1443
2901
1693
366
144
m/z
321.05
321.95
323 . 10
324 . 10
324 . 90
325 .20
325.95
326.20
326.95
328.10
329 . 00
. : W1002872 .D\data.
abund .
57
117
45
51
12456
1888
40
184
558
4186
1033
m/z
373.90
374.30
376.90
377 . 10
383 . 05
383 . 95
385 . 00
389.95
391.10
392.00
393.30
. : W1002872 .D\data.
abund .
40
37
73
83
52
262
61
231
35283
236523
45283
m/z
444.10
444.80
445.10
445.30
abund .
1203
284
36
361
899
699
1520
314
116
342
214
. ms
abund .
880
225
8598
1553
84
55
97
62
1558
913
132
. ms
abund .
90
57
63
35
1081
400
48
614
389
311
41
. ms
abund .
4210
61
190
34
m/z
291.05
291 . 90
292 . 10
293 . 05
294 . 00
296.05
297.10
298.10
299.70
300 . 85
301 . 10
m/z
332.00
333.05
334 . 05
335 . 05
335 . 90
336 .20
339.10
339.80
340.20
341.10
342 . 10
m/z
395.00
395.20
396.70
400 . 90
401 . 15
402 . 05
403 . 05
404.05
405.05
410.00
410.20
m/z
abund .
291
117
257
1842
360
26536
3759
176
38
165
147
abund .
593
840
5405
1336
42
127
45
67
61
1038
351
abund .
45
43
34
75
140
1261
2245
743
159
82
38
abund .
EA# 0111-13 Page 95 of 112
W101810X-8270.M Mon Oct 25 10:18:03 2010
Page: 3
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Quant Time Oct 25 10:14:04 2010
Quant Method : M:\ms2010\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Fri Sep 17 17:54:07 2010
Response via : Initial Calibration
Abundance
400000
300000
200000
100000
Ion 265.90 (265.60 to 266.60): W1002872.D\data.ms
Ion 267.90 (267.60 to 268.60): W1002872.D\data.ms
Ion 263.90 (263.60 to 264.60): W1002872.D\data.ms
9.644 Tailing = 1.09
Time-> 9.48 9.50 9.52 9.54 9.56 9.58 9.60 9.62 9.64 9.66 9.68 9.70 9.72 9.74 9.76 9.78 9.80 9.82 9.84 9.86 9.88 9.90
Abundance Scan 1247 (9.644 min): W1002872.D\data.ms
26^.9
300000]
250000
200000
150000
100000
50000
165.0
95.1
60.1
47.1
130.0
,108.0
143.0
201.9
178.9
229.9
215.9
.,i 842.8
281.1
356.2
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360
TIC: W1002872.D\data.ms
(1) Pentachlorophenol
9.644min (-0.101) 51.44ug/mL
response 314663
Ion Exp% Act%
265.90 100 100
267.90 65.10 62.69
263.90 65.90 61.44
0.00 0.00 0.00
EA# 0111-13 Page 96 of 112
DFTPP-4-EA.M Mon Oct 25 10:14:30 2010
Page: 1
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Quant Time Oct 25 10:14:04 2010
Quant Method : M:\ms2010\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Fri Sep 17 17:54:07 2010
Response via : Initial Calibration
Abundance
1400000
1200000
1000000
800000
600000
400000
200000
0
Ion 184.20 (183.90to 184.90): W1 002872. D\data.ms
Ion 185.20(184.90(0 185.90): W1 002872. D\data.ms
Ion 183.10(182.80(0 183.80): W1 002872. D\data.ms
11.172 Tailing = 0.90 |
A
\
'
'
J \ '
1/XV_ i
Time-> 11.00 11.02 11.04 11.06 11.08 11.10 11.12 11.14 11.16 11.18 11.20 11.22 11.24 11.26 11.28 11.30 11.32 11.34 11.36
Abundance Scan 1533 (11.172 min): W1002872.D\data.ms
1200000 j 18^-2
1000000
800000
600000
400000
200000
92.1
391
156.2
207.1
239.0
281.1
341.2
461.1
m/z-->
40 60
80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400
TIC: W1002872.D\data.ms
420 440 460
(3) Benzidine
11.172min (-0.101) 48.65 ug/mL
response 1263024
Ion Exp% Act%
184.20 100 100
185.20 13.60 14.18
183.10 12.20 11.72
0.00 0.00 0.00
EA# 0111-13 Page 97 of 112
DFTPP-4-EA.M Mon Oct 25 10:15:21 2010
Page: 1
-------
File
Operator
Acquired
Instrument
Sample Name
Misc Info
Vial Number
M:\ms2010Q4\wiley\data\octlO\W101810A\W1002872.D
dda
18 Oct 2010 5:33 pm using AcqMethod RXI-2A-EA.M
Wiley
DFTPP
Abundance
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
Time~>
TIC: W1002872.D\data.ms
D>D>T Breakdown / Degradation = 3.6%
32
9.60 9.80 10.00 10.20
,20 11.40 11.60 11.80 12.00 12.20
-------
TIC: Wl002872.D\data.ms
DFTPP
Peak # Ret Time Type Width Area Start Time End Time
1 11.359 rm 0.032 11072 11.343 11.375
2 11.701 rm 0.102 161495 11.674 11.775
3 12.032 rm 0.080 4671955 12.000 12.080
EA# 0111-13 Page 99 of 112
-------
DFTPP
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100041.D
28 Jan 2011 12 :33 pm
tdd
DFTPP
gcmsprep2pg41 exp 9/22/11
1 Sample Multiplier: 1
Integration File: rteint.p
Method : M:\ms2011\methods\wiley\W101810X-8270.M
Title : 8270C - Full List
Last Update : Wed Oct 20 14:58:44 2010
Abundance
6000000
5000000
4000000
3000000
2000000
1000000
Time~> 8.00
Abundance
4000001
300000
200000
100000
TIC: W1100041.D\data.ms
8.20 8.40 8.60 8.80
9.00 9.20 9.40 9.60 9.80 10.00 10.20 10.40 10.60 10.80 11.00 11.20 11.40 11.60 11.80
Average of 9.943 to 9.954 min.: W1100041 .D\data.ms (-)
198
127
69
51
39
110
81
93
m/z~>
186
148
167
255
442
224
275
296
308 323335 3523^5 383 403
J<—, , ''I ,• l| 'I ,~: ,• , ' , I" , , ,!' , •!• , , ' "I , ,
40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440
AutoFind: Scans 1303, 1304, 1305; Background Corrected with Scan 1296
Target
Mass
51
68
69
70
127
197
198
199
275
365
441
442
443
Rel. to
Mass
198
69
198
69
198
198
198
198
198
198
443
198
442
Lower
Limit%
30
0 . 00
0 . 00
0 . 00
40
0.00
100
5
10
1
0 . 01
40
17
Upper
Limit%
60
2
100
2
60
1
100
9
30
100
100
100
23
Rel.
Abn%
34.2
0 . 0
42 .4
0 . 6
50 .4
0.0
100.0
6.7
26.5
3 . 7
75 .3
61 . 1
19 . 6
Raw
Abn
131919
0
163562
949
194133
0
385515
25901
102325
14242
34712
235435
46108
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
EA# 0111-13 Page 100 of 112
W101810X-8270.M Fri Jan 28 18:49:28 2011
Page: 1
-------
Average of 9.943 to 9
DFTPP
Modified:subtracted
.954 min.: W1100041.D\data.ms
m/z
35 . 10
35 . 95
37 . 15
38 . 15
39.10
40.05
41.10
42.20
43 . 10
44 . 00
44 . 90
Average of <
DFTPP
abund .
100
44
859
2210
10720
499
383
36
167
149
72
3.943 to E
m/z
45 . 05
48 . 10
49 . 15
50 . 10
51.10
52.15
52.90
53.20
55 . 15
56 . 10
57 . 10
3.954 min.
abund .
195
41
1034
37277
131919
6883
39
204
750
4310
9762
: W1100041
m/z
58 . 10
59 . 10
59.90
60 .20
61.10
62.10
63.10
64.10
65 . 10
66 . 10
67 . 05
. D\data .ms
abund .
462
86
38
74
1821
2247
5868
906
2807
368
186
m/z
69 . 10
70 . 05
71 . 15
71 . 90
72.10
73.10
74.10
75.10
76 .20
77 . 10
78 . 10
abund .
163562
949
291
39
59
1505
16555
25037
8495
161843
11721
Modified : subtracted
m/z
79.10
80 . 10
81 . 10
82 . 10
83 . 10
84.00
85.05
86.05
87.15
87 . 90
88 . 10
Average of <
DFTPP
abund .
12475
9184
12767
3108
2853
118
2006
3815
1770
184
554
3.943 to E
m/z
89.05
90 . 10
91 . 00
92 . 10
93 . 10
94.05
95.15
96.00
97.05
98 . 10
99.05
3.954 min.
abund .
278
47
2805
3357
22087
1531
456
931
482
17922
12714
: W1100041
m/z
100.05
101 . 10
102 . 00
102 . 15
103 . 05
104.10
105.10
106.15
107.10
108 . 10
109 . 10
. D\data .ms
abund .
1215
6676
67
254
2283
4994
4304
1144
55165
8772
520
m/z
110.10
111 . 10
112 . 05
113 . 15
114 . 10
115.10
116.10
117.10
118.05
119 . 05
120 . 10
abund .
94275
14476
1912
662
49
317
3619
52416
3463
585
820
Modified : subtracted
m/z
121.00
121.30
122 . 05
123 . 10
124 . 05
125 . 10
127.10
128.10
129.10
130.05
131 . 05
Average of <
DFTPP
abund .
212
82
4160
6369
2774
2405
194133
15438
80136
7286
1469
3.943 to E
m/z
132.10
133.05
134 . 05
135 . 10
136 . 05
137 . 10
138.00
138.90
139.10
140.05
141 . 05
3.954 min.
abund .
601
223
2338
6558
2369
3219
440
247
105
1006
10917
: W1100041
m/z
142.05
143.05
144 . 05
145 . 05
146 . 05
147 . 10
148.00
149.05
150.05
151.10
151 . 70
. D\data .ms
abund .
3313
2360
775
568
1759
5601
12518
2516
641
1361
423
m/z
152.00
152.30
153 . 05
154 . 05
155 . 10
156 . 10
157.05
157.90
158.05
159.10
160 . 05
abund .
233
176
3298
2224
5803
8436
1558
357
1675
1353
3420
Modified : subtracted
m/z
161.10
162.10
163.15
164 . 10
165 . 00
166 . 05
167 . 05
168.10
169.05
170.05
171.00
Average of <
DFTPP
abund .
4623
1406
431
625
4264
3167
20988
9407
1916
744
1043
3.943 to E
m/z
172.05
173.10
174.05
175 . 10
176 . 05
177 . 05
178 . 05
179.00
180.10
181.00
181.90
3.954 min.
abund .
2188
2526
4580
7901
2491
3608
1549
14963
10164
4648
173
: W1100041
m/z
182.10
182.60
183.15
184 . 05
185 . 10
186 . 10
187 . 10
188.05
189.05
189.90
190.05
. D\data .ms
abund .
721
121
520
1378
7675
56691
15409
2128
3752
155
496
m/z
190.90
191.05
192.05
193 . 05
194 . 00
195 . 05
196 . 05
198.00
199.00
200.00
201.60
abund .
295
1113
5072
5609
1316
739
12249
385515
25901
1958
2013
Modified : subtracted
m/z
203.05
204.10
205.10
206.10
207 . 10
208 . 05
209 . 00
210 . 15
211.05
212.00
212.20
Average of <
abund .
3211
14289
24612
104600
13382
3562
1492
1565
4503
541
117
3.943 to E
m/z
213.05
213.70
214.10
215.00
216 . 10
217 . 00
218 . 05
219 . 05
219.40
219.70
221.05
3.954 min.
abund .
349
36
46
1279
2384
29152
3586
435
145
51
21062
: W1100Q41,
m/z
221.90
223.00
224.10
225.10
226 . 10
227 . 00
228 . 00
229 . 05
229.90
230.10
231.05
.DAjdata-Kis.
abund .
778
6147
57403
14877
1699
26979
3905
5560
132
545
2012
~3 m-is^ -i n -i
m/z
232.05
233.05
234.05
235.05
236 . 00
237 . 05
237 . 90
238 . 15
239.05
240.15
241.05
*f -\ -\ O
abund .
282
524
1676
1700
1004
2322
174
143
1043
908
1523
W101810X-8270.M Fri Jan 28 18:49:28 2011
Page: 2
-------
DFTPP
Modified:subtracted
m/z
242.05
243 . 05
244 . 10
245 . 10
246 . 00
247.05
248.05
249.00
250.00
251 . 05
252 . 00
abund .
3256
3137
45864
5789
9668
1851
476
1679
425
619
207
Average of 9.943 to E
DFTPP
Modified:
m/z
288.20
288.95
290 . 05
291 . 05
292 . 10
293 . 05
294.05
295.00
296.00
297.00
297 . 95
subtracted
abund .
49
306
364
284
532
1881
608
104
33421
4696
264
Average of 9.943 to S
DFTPP
Modified:
m/z
334.10
335.05
335.90
336 .25
338 . 90
339 .20
339.95
340.90
341.05
342.05
346.00
subtracted
abund .
6027
1548
65
192
91
58
133
156
892
220
1924
Average of 9.943 to E
DFTPP
Modified:
m/z
392.10
392.90
395.00
397.20
401 . 00
401 .20
402 . 00
403 . 10
404.05
405.05
406.00
subtracted
abund .
214
41
74
44
185
137
1584
2259
729
206
47
m/z
252.20
253 . 05
255 . 05
256 . 05
257 . 05
258.00
259.05
260.00
260.95
262 . 00
263 . 00
3.954 min.
m/z
301.05
302.10
303 . 10
304 . 05
304 . 70
305 . 05
308.00
308.95
309.90
310.05
311 . 10
3.954 min.
m/z
347.00
347.90
350.00
351 . 05
352 . 05
353 . 05
354 . 05
354.90
355.10
356.10
356.90
3.954 min.
m/z
406.70
410.10
413.40
415.00
419 . 00
420 . 90
421 . 05
422 . 10
423.10
424.05
425.05
abund .
281
1001
228117
33875
2792
15322
2767
441
405
44
124
: W1100041
abund .
535
612
4075
1034
50
155
491
294
81
368
77
: W1100041
abund .
389
46
45
125
2784
1850
3029
342
244
99
37
: W1100041
abund .
51
116
40
77
35
293
1529
1917
12525
3075
384
m/z
263.90
265 . 00
265 . 95
266 . 95
267 . 80
270.00
270.95
272.05
273.00
274 . 05
275 . 00
. D\data . ms
m/z
311.95
312.90
313 . 10
314 . 00
315 . 05
316 . 05
317.05
320.00
321.15
322.10
323 . 10
. D\data .ms
m/z
358.10
359.05
360.20
361 . 15
363 . 70
364 . 00
365 . 00
366.00
367.00
370.10
371.05
. D\data .ms
m/z
427.20
429.10
431.20
432.30
435 . 00
435 .40
436 . 00
436 . 75
437.55
437.90
438.30
abund .
408
6320
1049
140
189
284
473
676
6776
18067
102325
abund .
152
83
165
1344
3791
2211
388
142
993
320
9626
abund .
58
309
51
68
53
48
14242
1910
243
341
641
abund .
46
42
45
51
42
49
36
136
216
131
119
m/z
276.05
277 . 00
278 . 05
279 . 00
281 . 00
282.00
283.00
284.15
285.10
286 . 10
288 . 00
m/z
324.10
325.00
325 .30
326 . 10
327 . 05
328 . 00
329.00
330.10
330.40
332.00
333 . 05
m/z
372.10
373.00
374.05
377 . 00
383 . 05
383 . 80
384 . 10
385.05
385.90
390.05
390.95
m/z
438.60
438.85
439.20
439.90
441 . 10
442 . 10
443 . 10
444 . 05
445.05
abund .
13798
9335
1601
447
255
261
1095
872
1693
382
44
abund .
1773
47
83
167
1755
990
153
40
34
765
987
abund .
4398
985
171
127
1017
41
252
126
38
665
424
abund .
57
509
85
51
34712
235435
46108
4102
268
EA# 0111-13 Page 102 of 112
W101810X-8270.M Fri Jan 28 18:49:28 2011
Page: 3
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100041.D
28 Jan 2011 12 :33 pm
tdd
DFTPP
gcmsprep2pg41 exp 9/22/11
1 Sample Multiplier: 1
Quant Time Jan 28 12:50:30 2011
Quant Method : M:\ms2011\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Thu Dec 30 11:51:21 2010
Response via : Initial Calibration
Ion 265.90 (265.60 to 266.60): W1100041 .D\data.ms
Ion 267.90 (267.60 to 268.60): W1100041 .D\data.ms
Ion 263.90 (263.60 to 264.60): W1100041 .D\data.ms
9.564 Jailing = 0.98
Abundance
400000
350000
300000
250000
200000
150000
100000
50000
0
Time-> 9.42 9.44 9.46 9.48 9.50 9.52 9.54 9.56 9.58 9.60 9.62 9.64 9.66 9.68 9.70 9.72 9.74 9.76 9.78 9.80 9.82 9.84 9.86
Abundance Scan 1232 (9.564 min): W1100041 .D\data.ms
300000
250000
200000
150000
100000
50000
n
95
60
35 47
i .
7
1
83
[
16
130
107 118
i.jln ilni
141
155
I . . . i, i
5
202
, 177 193,
2«
230
I, 214
?39 OEI-3
I I I i^OO
6
|,| 281 341 355
m/z->
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360
TIC: W1100041.D\data.ms
(1) Pentachlorophenol
9.564min (-0.016) 67.69 ug/mL
response 342184
Ion Exp% Act%
265.90 100 100
267.90 65.10 64.50
263.90 65.90 63.41
0.00 0.00 0.00
EA# 0111-13 Page 103 of 112
DFTPP-4-EA.M Fri Jan 28 18:50:42 2011
Page: 1
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\janll\W012811A\
W1100041.D
28 Jan 2011 12 :33 pm
tdd
DFTPP
gcmsprep2pg41 exp 9/22/11
1 Sample Multiplier: 1
Quant Time Jan 28 12:50:30 2011
Quant Method : M:\ms2011\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Thu Dec 30 11:51:21 2010
Response via : Initial Calibration
Abundance
1000000
800000
600000
400000
200000
Ion 184.20 (183.90 to 184.90): W1100041 .D\data.ms
Ion 185.20 (184.90 to 185.90): W1100041 .D\data.ms
Ion 183.10 (182.80 to 183.80): W1100041 .D\data.ms
11.092 [Tailing = 1.04
Time-> 10.98 11.00 11.02 11.04 11.06 11.08 11.10 11.12 11.14 11.16 11.18 11.20 11.22 11.24 11.26 11.28 11.30 11.32
Abundance Scan 1518 (11.092 min): W1100041.D\data.ms
800000]
700000
600000
500000
400000
300000
200000
100000
39 52 65 77
92
156
102
167
176
196 207
227 239 257 267 281
312
341
30 40 50 60 70 80 90100110120130140150160170180190200210220230240250260270280290300310320330340
TIC: W1100041.D\data.ms
(3) Benzidine
11.092min (-0.016) 52.16 ug/mL m
response 848824
Ion Exp% Act%
184.20 100 100
185.20 13.60 14.78
183.10 12.20 12.26
0.00 0.00 0.00
EA# 0111-13 Page 104 of 112
DFTPP-4-EA.M Fri Jan 28 18:50:14 2011
Page: 1
-------
File
Operator
Acquired
Instrument
Sample Name
Misc Info
Vial Number
M:\ms2011ql\wiley\data\janll\W012811A\W1100041 .D
tdd
28 Jan 2011 12:33 pm using AcqMethod RXI-2A-EA.M
Wiley
DFTPP
gcmsprep2pg41 exp 9/22/11
1
Abundance
6000000
5800000
5600000
5400000
5200000
5000000
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
Time~>
TIC: W1100041.D\data.ms
11
DDT Breakdown / Degradation = 2.4%
1 1 .620
41
9.60
9.80
10.00
10.
11.20 11.40 11.60 11.80 12.00
-------
TIC: wll00041.D\data.ms
DFTPP
Peak # Ret Time Type Width Area Start Time End Time
1 11.279 rm 0.043 14444 11.257 11.300
2 11.620 rm 0.091 137342 11.588 11.679
3 11.941 rm 0.096 6055343 11.904 12.000
EA# 0111-13 Page 106 of 112
-------
Sequence Name: M:\ms2010q4\wiley\sequence\w101810a
Operator: ede
Datapath: C:\ms2010q4\wiley\data\oct10\w101810a
Line Type
Vial DataFile
Method
Sample Name
Additional Info
Comments
1 DFTPP
2 DFTPP
3 DFTPP
4 Calibration
5 Sample
6 Blank
7 Calibration
8 Calibration
9 Calibration
10 DFTPP
11 Calibration
12 Calibration
13 Calibration
14 Calibration
15 Calibration
16 Calibration
17 Calibration
18 Calibration
19 Blank
20 Sample
21 Sample
22 Sample
23 Sample
1
1
1
2
4
5
2
2
2
1
6
7
8
9
10
11
12
13
2
16
17
18
19
W1 002863
W1 002864
W1 002865
W1 002866
W1 002867
W1 002868
W1 002869
W1 002870
W1 002871
W1 002872
W1 002873
W1 002874
W1 002875
W1 002876
W1 002877
W1 002878
W1 002879
W1 002880
W1 002881
W1 002882
W1 002883
W1 002884
W1 002885
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
DFTPP
DFTPP
DFTPP
SSTD005-SCAN
High Cone Mega Mix
CH2CI2 System Blank
SSTD005-SCAN
SSTD005-SCAN
SSTD005-SCAN
DFTPP
SSTD005-SCAN
SSTD010-SCAN
SSTD020-SCAN
SSTD050-SCAN
SSTD080-SCAN
SSTD120-SCAN
SSTD160-SCAN
SSTD050-SCAN (ICV)
CH2CI2 System Blank
IDOC EDE "A"
IDOC EDE "B"
IDOC EDE "C"
IDOC EDE "D"
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg14
QA-BS-1pg72 M8270-BS "A"
QA-BS-1pg72 M8270-BS "B"
QA-BS-1pg72 M8270-BS "C"
QA-BS-1pg72 M8270-BS "D"
EA# 0111-13 Page 107 of 112
-------
Sequence Name: M:\ms2011q1\wiley\sequence\w012811a
Operator: tdd
Datapath: C:\ms2011q1\wiley\data\jan11\w012811a
Line Type Vial DataFile
Method
Sample Name
Additional Info
DFTPP
DFTPP
DailyCal
Spike
Spike
Blank
Blank
Blank
Blank
10 Sample
11 Sample
12 Sample
13
1
1
2
3
4
5
6
7
8
9
10
11
W1100040
W1100041
W1100042
W1100043
W1100044
W1100045
W1100046
W1100047
W1100048
W1100049
W1100050
W1100051
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXISIM2A-EA
RXISIM2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
DFTPP
DFTPP
sstdOSO M8270 Std
0111-13 LCS
0111-13LCS(Conf)
CH2CI2 System Blank (Scan)
SVAOPrep1Pg179A Xad Blk
CH2CI2 System Blank (SIM)
SVAOPrep1Pg179A Xad Blk
0111-13 MB
0111-13 Run 1
0111-13 Run 1 LD
gcmsprep2pg41 exp 9/22/11
gcmsprep2pg41 exp 9/22/11
Scan Blank Check
SIM Blank Check
EA# 0111-13 Page 108 of 112
-------
INSTRUMENT CONTROL PARAMETERS:
Wiley
M:\MS2010\METHODS\WILEY-EA\RXI-2A-EA.M
Thu Jun 10 12:52:48 2010
Control Information
Sample Inlet : GC
Injection Source : GC ALS
Mass Spectrometer : Enabled
6890 GC METHOD
OVEN
Initial temp: 35 'C (On)
Initial time: 0.50 min
Ramps:
# Rate Final temp Final time
1 25.00 310 0.00
2 4.00 330 2.00
3 O.O(Off)
Post temp: 0 'C
Post time: 0.00 min
Run time: 18.50 min
Maximum temp: 450
Equilibration time:
0.20 min
FRONT INLET (SPLIT/SPLITLESS)
Mode: Pulsed Splitless
Initial temp: 250 'C (On)
Pressure: 7.91 psi (On)
Pulse pressure: 30.0 psi
Pulse time: 0.40 min
Purge flow:
Purge time:
Total flow:
Gas saver:
Saver flow:
Saver time:
Gas type:
50.0 mL/min
0.30 min
54.0 mL/min
On
20.0 mL/min
1.00 min
Helium
BACK INLET (SPLIT/SPLITLESS)
Mode: Split
Initial temp: 50 'C (Off)
Pressure: 11.25 psi (On)
Split ratio: 50:1
Split flow: 43.6 mL/min
Total flow:
Gas saver:
Saver flow:
Saver time:
mL/min
51.!
On
20.0 mL/min
2.00 min
Gas type: Hydrogen
COLUMN 1
Capillary Column
Model Number: Restek 13638
Rxi-5Sil MS, 30m x 0.25mm x 0.Sum
Max temperature: 350 'C
Nominal length: 30.0 m
Nominal diameter: 250.00 urn
Nominal film thickness: 0.50 urn
Mode: constant flow
Initial flow: 1.1 mL/min
Nominal init pressure: 7.92 psi
Average velocity: 38 cm/sec
Inlet: Front Inlet
Outlet: MSB
Outlet pressure: vacuum
COLUMN 2
Capillary Column
Model Number: Restek 40111
Rtx-1 20m x 0.18mm x 0.4 urn
Max temperature: 340 'C
Nominal length: 20.0 m
Nominal diameter: 180.00 urn
Nominal film thickness: 0.40 urn
Mode: constant flow
Initial flow: 0.9 mL/min
Nominal init pressure: 11.25 psi
Average velocity: 42 cm/sec
Inlet: Back Inlet
Outlet: Back Detector
Outlet pressure: ambient
FRONT DETECTOR (NO DET)
BACK DETECTOR (FID)
Temperature: 250 'C (On)
Hydrogen flow: 40.0 mL/min (Off)
Air flow: 450.0 mL/min (Off)
Mode: Constant makeup flow
Makeup flow: 45.0 mL/min (Off)
Makeup Gas Type: Nitrogen
Flame: Off
Electrometer: On
Lit offset: 2.0
EA# 0111-13 Page 109 of 112
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
Page: 2
-------
SIGNAL 1
Data rate: 50 Hz
Type: mux'ed adc offset
Save Data: Off
Zero: 0.0 (Off)
Range: 0
Fast Peaks: Off
Attenuation: 0
SIGNAL 2
Data rate: 50 Hz
Type: mux'ed adc offset
Save Data: Off
Zero: 0.0 (Off)
Range: 0
Fast Peaks: Off
Attenuation: 0
COLUMN COMP 1
Derive from back detector
COLUMN COMP 2
Derive from back detector
THERMAL AUX 2
Use: MSD Transfer Line Heater
Description:
Initial temp: 280 'C (On)
Initial time: 0.00 min
# Rate Final temp Final time
1 O.O(Off)
POST RUN
Post Time: 0.00 min
TIME TABLE
Time
Specifier
Parameter & Setpoint
GC Injector
Front Injector:
Sample Washes
Sample Pumps
Injection Volume
Syringe Size
Prelnj Solvent A Washes
Prelnj Solvent B Washes
Postlnj Solvent A Washes
Postlnj Solvent B Washes
Viscosity Delay
Plunger Speed
Prelnjection Dwell
Postlnjection Dwell
Sampling Depth
Back Injector:
No parameters specified
1
3
0.20 microliters
10.0 microliters
0
0
5
5
2 seconds
Fast
0.00 minutes
0.00 minutes
2 . 0 mm
Column 1 Inventory Number
Column 2 Inventory Number
General Information
13638
40111
MS ACQUISITION PARAMETERS
Tune File
Acquistion Mode
dftpp060710d.u
Scan
MS Information
Solvent Delay
EMV Mode
Gain Factor
Resulting EM Voltage
2.90 min
Gain Factor
1.00
1588
EA# 0111-13 Page 110 of 112
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
Page: 3
-------
[Scan Parameters]
Low Mass
High Mass
Threshold
Sample #
[MSZones]
MS Source
MS Quad
35.0
500 . 0
100
2
A/D Samples
230 C maximum 250 C
150 C maximum 200 C
END OF MS ACQUISITION PARAMETERS
TUNE PARAMETERS for SN: US21844806
Trace Ion Detection is OFF.
EMISSION
ENERGY
REPELLER
IONFOCUS
ENTRANCE LE
EMVOLTS
AMUGAIN
AMUOFFSET
FILAMENT
DCPOLARITY
ENTLENSOFFS
14.306@502
MASSGAIN
MASSOFFSET
34.610
69.922
34.814
90.157
0.000
1541.176
2060.000
132.000
1.000
0.000
12.800®
14.306@799
69 . 000
-10.000
Actual EMV
GAIN FACTOR
12.800® 50
1588.23
1 . 02
16.314® 69
16.816@131
20.831@219
14.306@414
END OF TUNE PARAMETERS
END OF INSTRUMENT CONTROL PARAMETERS
EA# 0111-13 Page 111 of 112
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
Page: 4
-------
This Is The Last Page
Of This Report.
** '
EA# 0111-13 Page 112 of 112
-------
ARCADIS-US: Durham
4915 Prospectus Drive, Suite F
Durham, NC 27713
ACB Outlet Duct
Project^ RN990271.0002
Analytical Report
(0111-20)
EPA SW-846 Method 827OC
20 Polyaromatic Hydrocarbons - SCAN Mode
Enthalpy Analytical, Inc.
^"jAi
|*,;fl^ B Phone: (919) 850 - 4392 / Fax: (919) 850 - 9012 / www.enthalpy.com
,**•* '} 2202 Ellis Road Durham, NC 27703 - 5518
-------
I certify that to the best of my knowledge all analytical data presented in this report:
• Have been checked for completeness
• Are accurate, error-free, and legible
• Have been conducted in accordance with approved protocol, and that all deviations and
analytical problems are summarized in the appropriate narrative(s)
This analytical report was prepared in Portable Document Format (.PDF) and contains 126 pages.
QA Review Performed by - Valgena Respass
Report Issued: 03/03/2011
,M»1 ifY-M'
J^*"" .^-"Hjj1""*
^_^
EA# 0111-20 Page 2 of 126
-------
Results
EA# 0111-20 Page 3 of 126
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run& 1 FB
Client*
Job*
P0#
Report Date
RN990271.0002
0111-20
RB68316
2/21/2011
Sample ID: 0111-20 1R-ACB-M0010-02
Data File: W1100171.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 10.0
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
6.48
7.15
7.26
8.07
8.24
8.76
9.75
9.80
11.0
11.2
12.5
12.5
14.0
14.0
14.5
14.6
NA
16.6
NA
17.2
5.25
6.45
8.21
9.72
12.5
14.7
4.13
4.86
5.74
7.50
8.73
9.01
11.2
11.3
Area
6,646,853
529,527
410,185
1,597,473
49,789
366,177
1,296,702
285,592
639,953
555,852
132,322
118,034
118,581
42,266
43,800
76,972
0
40,344
0
35,945
685,096
2,422,513
1,384,296
2,621,296
2,655,817
2,218,030
160,531
217,752
229,008
429,091
312,299
61,385
612,630
494,933
Cone.
(ucj/mL)
114
14.2
11.4
28.7
1.40
9.18
21.9
4.83
10.4
8.40
2.06
1.95
2.14
0.780
0.886
1.51
0.340
0.800
0.425
0.874
40.0
40.0
40.0
40.0
40.0
40.0
8.59
10.0
10.7
10.0
8.22
10.2
11.0
11.4
Catch
Weight
(ug)
1,139
142
114
287
14.0
91.8
219
48.3
104
84.0
20.6
19.5
21.4
7.80
8.86
15.1
3.40
8.00
4.25
8.74
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
85.9
100
107
100
82.2
102
110
114
Flags
J
J
J
J
J
J
J
J
ND
J
ND
J
Spk
Amt
100
100
100
100
100
100
100
100
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.25
6.46
8.21
9.72
12.50
14.66
Sample
RT
5.25
6.45
8.21
9.72
12.50
14.66
Concal
IS Area
768,718
2,761,610
1,657,156
3,022,233
2,979,857
2,352,403
Sample
IS Area
685,096
2,422,513
1,384,296
2,621,296
2,655,817
2,218,030
Flag
Pass
Pass
Pass
Pass
Pass
Pass
0111 -20 pah summaries
EA# 0111-20 Page 4 of 126
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run& 1 FB
Client*
Job*
P0#
Report Date
RN990271.0002
0111-20
RB68316
2/21/2011
Sample ID: 0111-20 1R-ACB-M0010-02 LD
Data File: W1100172.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 10.0
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
6.48
7.15
7.26
8.07
8.24
8.76
9.75
9.80
11.0
11.2
12.5
12.5
14.0
14.0
14.5
14.6
NA
16.6
NA
17.2
5.25
6.45
8.21
9.72
12.5
14.7
4.13
4.87
5.74
7.50
8.73
9.01
11.2
11.3
Area
8,221,858
690,477
546,423
2,093,917
62,647
483,637
1,686,639
371,108
804,904
691 ,504
155,187
138,276
138,735
41,874
50,794
86,534
0
43,017
0
38,236
897,371
3,211,606
1,892,982
3,475,619
3,264,792
2,597,182
209,295
272,058
287,163
566,923
413,838
81,678
752,659
621 ,747
Cone.
(ucj/mL)
106
13.9
11.4
27.5
1.29
8.87
21.5
4.74
9.86
7.88
1.97
1.86
2.14
0.660
0.878
1.45
0.340
0.728
0.425
0.794
40.0
40.0
40.0
40.0
40.0
40.0
8.55
9.56
10.3
9.69
7.96
10.3
10.4
10.8
Catch
Weight
(ug)
1,063
139
114
275
12.9
88.7
215
47.4
98.6
78.8
19.7
18.6
21.4
6.60
8.78
14.5
3.40
7.28
4.25
7.94
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
85.5
95.6
103
96.9
79.6
103
104
108
Flags
J
J
J
J
J
J
J
J
ND
J
ND
J
Spk
Amt
100
100
100
100
100
100
100
100
% Diff
6.7
1.6
0.5
4.1
8.0
3.4
1.9
2.0
5.1
6.2
4.6
4.7
0.1
15.4
1.0
4.0
0.0
8.9
0.0
9.2
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.25
6.46
8.21
9.72
12.50
14.66
Sample
RT
5.25
6.45
8.21
9.72
12.50
14.66
Concal
IS Area
768,718
2,761,610
1,657,156
3,022,233
2,979,857
2,352,403
Sample
IS Area
897,371
3,211,606
1,892,982
3,475,619
3,264,792
2,597,182
Flag
Pass
Pass
Pass
Pass
Pass
Pass
0111 -20 pah summaries
EA# 0111-20 Page 5 of 126
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run& 1 FB
Client*
Job*
P0#
Report Date
RN990271.0002
0111-20
RB68316
2/21/2011
Sample ID: 0111-20 ACB-M0010-FB
Data File: W1100167.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5.24
6.45
8.21
9.72
12.5
14.7
4.11
4.84
5.74
7.50
8.73
9.00
11.2
11.3
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
725,663
2,519,480
1,421,101
2,538,416
2,681,283
2,484,829
300,841
346,781
353,511
825,474
614,909
91,293
1,152,403
918,194
Cone.
(ucj/mL)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
40.0
40.0
40.0
40.0
40.0
40.0
15.2
15.1
15.6
18.8
15.8
15.7
21.8
21.8
Catch
Weight
(ug)
3.33
3.09
3.07
3.37
4.67
3.92
3.86
3.80
4.60
4.69
2.75
2.78
1.50
1.78
1.70
1.70
1.70
1.50
2.13
1.76
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
76.0
75.4
78.2
93.9
78.8
78.5
109
109
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Spk
Amt
100
100
100
100
100
100
100
100
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.25
6.46
8.21
9.72
12.50
14.66
Sample
RT
5.24
6.45
8.21
9.72
12.50
14.65
Concal
IS Area
768,718
2,761,610
1,657,156
3,022,233
2,979,857
2,352,403
Sample
IS Area
725,663
2,519,480
1,421,101
2,538,416
2,681,283
2,484,829
Flag
Pass
Pass
Pass
Pass
Pass
Pass
0111 -20 pah summaries
EA# 0111-20 Page 6 of 126
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run& 1 FB
Client*
Job*
P0#
Report Date
RN990271.0002
0111-20
RB68316
2/21/2011
Sample ID: 0111-20 MB
Data File: W1100166.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 5.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
112
99
82
172
176
330
212
244
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5.24
6.45
8.21
9.72
12.5
14.7
4.11
4.84
5.74
7.50
8.73
9.00
11.2
11.3
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
670,592
2,325,282
1,339,713
2,297,128
2,342,775
2,147,243
250,857
308,078
275,269
679,603
540,343
81,848
991 ,551
770,215
Cone.
(ucj/mL)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
40.0
40.0
40.0
40.0
40.0
40.0
13.7
14.5
13.2
16.4
14.7
15.6
20.7
20.2
Catch
Weight
(ug)
3.33
3.09
3.07
3.37
4.67
3.92
3.86
3.80
4.60
4.69
2.75
2.78
1.50
1.78
1.70
1.70
1.70
1.50
2.13
1.76
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
68.6
72.4
65.9
82.0
73.4
77.8
104
101
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Spk
Amt
100
100
100
100
100
100
100
100
Flags
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.25
6.46
8.21
9.72
12.50
14.66
Sample
RT
5.24
6.45
8.21
9.72
12.50
14.66
Concal
IS Area
768,718
2,761,610
1,657,156
3,022,233
2,979,857
2,352,403
Sample
IS Area
670,592
2,325,282
1,339,713
2,297,128
2,342,775
2,147,243
Flag
Pass
Pass
Pass
Pass
Pass
Pass
0111 -20 pah summaries
EA# 0111-20 Page 7 of 126
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method 8270C
1 Run& 1 FB
Client*
Job*
P0#
Report Date
RN990271.0002
0111-20
RB68316
2/21/2011
Sample ID: CH2CI2 System Blank
Data File: W1100164.D
Tank/Misc ID:
DF: 1.00
Aliquot Factor: 1.00
Extraction Vol (ml): 1.00
ConCal 1
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Quant
128
142
142
152
154
166
178
178
202
202
228
228
252
252
252
252
252
276
278
276
152
136
164
188
240
264
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
5.24
6.45
8.21
9.72
12.5
14.7
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
723,138
2,495,204
1,439,095
2,532,591
2,447,876
2,164,661
Cone.
(ucj/mL)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
40.0
40.0
40.0
40.0
40.0
40.0
Catch
Weight
(ug)
0.665
0.618
0.613
0.675
0.934
0.783
0.771
0.760
0.919
0.937
0.550
0.556
0.300
0.356
0.340
0.340
0.340
0.300
0.425
0.352
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Spk
Amt
Internal Standard Acceptance Criteria (Area -50%/+100%, RT +/- 30 sec.)
Internal Standard
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
Concal
RT
5.25
6.46
8.21
9.72
12.50
14.66
Sample
RT
5.24
6.45
8.21
9.72
12.50
14.66
Concal
IS Area
768,718
2,761,610
1,657,156
3,022,233
2,979,857
2,352,403
Sample
IS Area
723,138
2,495,204
1,439,095
2,532,591
2,447,876
2,164,661
Flag
Pass
Pass
Pass
Pass
Pass
Pass
0111 -20 pah summaries
EA# 0111-20 Page 8 of 126
2/22/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method 8270C
# Samples 1 Run & 1 FB
Sample ID: 0111-20 LCS
Data File: W1100158.D
Tank/Misc ID:
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Client*
Job*
P0#
Report Date
RN990271
0111-20
RB68316
2/21/2011
0002
DF:
Aliquot Factor:
Extraction Vol (mL):
Ret.
Quant Time
(min'
128 6.48
142 7.15
142 7.26
152 8.07
154 8.24
166 8.76
178 9.75
178 9.80
202 1 1 .0
202 1 1 .2
228 12.5
228 12.5
252 14.0
252 14.0
252 14.5
252 14.6
252 14.7
276 16.7
278 16.7
276 17.2
152 5.25
136 6.45
164 8.21
188 9.72
240 12.5
264 14.7
112 4.11
99 4.84
82 5.74
172 7.50
176 8.73
330 9.00
212 11.2
244 1 1 .3
Area
1,174,237
780,660
756,707
1,228,078
795,545
894,472
1,327,957
1,308,700
1,441,230
1,563,122
1,460,297
1,377,168
1,307,149
1,346,723
1,295,478
1,262,471
1,324,734
1,264,804
1,052,808
1,021,882
807,525
2,791,269
1,650,724
2,996,877
3,059,085
2,714,151
323,591
407,923
427,798
974,679
779,205
135,784
1,363,687
1,114,833
Cone
(ug/mL)
17.5
18.1
18.2
18.5
18.7
18.8
19.6
19.4
20.5
20.7
19.8
19.8
19.3
20.3
21.4
20.2
20.3
20.5
20.2
20.3
40.0
40.0
40.0
40.0
40.0
40.0
14.7
15.9
17.0
19.1
17.2
19.8
21.8
22.4
ConCal 1
Catch
Weight
(ug)
87.3
90.7
90.9
92.5
93.7
94.1
98.1
96.9
102
103
98.9
99.0
96.3
102
107
101
102
102
101
101
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
73.5
79.7
85.1
95.5
86.0
98.9
109
112
1.00
1.00
5.00
Flags
Spk
Amt
100
100
100
100
100
100
100
100
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method 8270C
# Samples 1 Run & 1 FB
Sample ID: 0111-20 LCSD
Data File: W1100160.D
Tank/Misc ID: Reprep
Compound
Naphthalene
2-Methylnaphthalene
1 -Methylnaphthalene
Acenaphthylene
Acenaphthene (CCC)
Fluorene
Phenanthrene
Anthracene
Fluoranthene (CCC)
Pyrene
Benzo(a)anthracene
Chrysene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(e)pyrene
Benzo(a)pyrene (CCC)
Perylene
lndeno(1 ,2,3-cd)pyrene
Dibenz(a,h)anthracene
Benzo(g,h,i)perylene
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
2-Fluorophenol (surr)
Phenol-d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-d10 (surr)
2,4,6-Tribromophenol (surr)
Pyrene-d10 (surr)
Terphenyl-d14 (surr)
Client*
Job*
P0#
Report Date
RN990271
0111-20
RB68316
2/21/2011
0002
DF:
Aliquot Factor:
Extraction Vol (mL):
Ret.
Quant Time
(min'
128 6.48
142 7.15
142 7.26
152 8.07
154 8.24
166 8.76
178 9.75
178 9.80
202 1 1 .0
202 1 1 .2
228 12.5
228 12.5
252 14.0
252 14.0
252 14.5
252 14.6
252 14.7
276 16.7
278 16.7
276 17.2
152 5.25
136 6.45
164 8.21
188 9.72
240 12.5
264 14.7
112 4.11
99 4.84
82 5.74
172 7.50
176 8.73
330 9.00
212 11.2
244 1 1 .3
Area
1,019,040
699,687
681,128
1,105,850
695,387
809,596
1,152,513
1,158,166
1,203,866
1,302,584
1,140,788
1,079,996
1,070,422
1,028,663
1,009,827
985,394
944,939
918,701
770,875
746,659
730,272
2,523,281
1,479,378
2,632,375
2,529,691
2,231,292
266,783
355,702
371 ,886
866,000
710,080
112,897
1,125,850
91 1 ,997
Cone
(ug/mL)
16.8
18.0
18.1
18.6
18.3
19.0
19.4
19.5
19.5
19.6
18.7
18.8
19.2
18.9
20.3
19.2
17.7
18.1
18.0
18.0
40.0
40.0
40.0
40.0
40.0
40.0
13.4
15.4
16.4
18.9
17.5
18.7
20.5
20.9
ConCal 1
Catch
Weight
(ug)
83.8
89.9
90.5
92.9
91.4
95.0
97.0
97.6
97.3
98.1
93.4
93.9
95.9
94.4
102
96.0
88.3
90.5
90.0
90.2
Rec. (%)
N/A
N/A
N/A
N/A
N/A
N/A
67.0
76.8
81.8
94.7
87.4
93.7
103
104
1.00
1.00
5.00
Flags
Spk
Amt
100
100
100
100
100
100
100
100
-------
Narrative Summary
EA# 0111-20 Page 11 of 126
-------
Enthalpy Analytical Narrative Summary
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA SW-846 Method
8270C (semi-volatile)
2
Client #
Job#
PO#
Report Date
RN99027 1.0002
0111-20
RB68316
February 22, 2011
Custody Heather Tarjeft received the samples on 2/17/11 after being relinquished
by ARCADIS-US: Durham. The samples were received at 18.3°C and
in good condition. Prior to, during, and after analysis, the sample was
kept under lock with access only to authorized personnel by Enthalpy
Analytical, Inc.
Analysis The samples were analyzed for SW-846 Method 8270C Polyaromatic
Hydrocarbons (PAHs) using the analytical procedures in EPA SW-846
Method 827 OC, Semivolatile Organic Compounds by Gas
Chromatography/Mass Spectrometry (GC/MS).
Prior to sample collection, the XAD-2 traps were spiked with a BN
Surrogate Mix (100 jig of nitrobenzene-ds, 2-fluorobiphenyl, and
terphenyl-di/t).
Each sample train consisted of five fractions: Filter, XAD Trap, FH
Rinse, BH Rinse, and Condensate. All rinse fractions were
methanol/methylene chloride.
Prior to extraction, each Filter/XAD fraction was spiked with acid
extraction surrogates (100 jig of 2-fluorophenol, phenol-ds, and 2, 4, 6-
tribromophenol) and PAH extraction surrogates (fluorene-dio and
pyrene-dio).
The filter and XAD-2 fractions for each sample were Soxhlet extracted
for 18 hours. The rinse and condensate fractions were combined and
Separately funnel extracted using methylene chloride.
The Condensate/Rinse and Filter/XAD-2 extracts for each sample
(including QC samples) were combined and concentrated to a final
volume of 5mL, with expectation of 1R-ACB-M0010-02, which was
concentrated to a final volume lOmL.
A lOOjiL aliquot of each sample extract was spiked with SVGA internal
standard spiking solution, before analyses.
The Agilent Technologies Model 6890N, Gas Chromatograph "Wiley"
(S/N CN10244010) was equipped with a 5973 Mass Selective Detector
and a Restek rxi-5Sil MS, 30m x 0.25mm x 0.5|im capillary column
(SN 982029) for these analyses.
•H'
EA# 0111-20 Page 12 of 126
-------
Enthalpy Analytical Narrative Summary
(continued)
Calibration The RSD of all target compounds in the initial calibration were within
the 20% acceptance limit.
All continuing calibration criteria were met in the analysis of these
samples.
The calibration curve is included in the Calibration Curve
Chromatograms section of this report.
Chromatographic A copy of the acquisition method RXI-2A-EA is included in the
Conditions Calibration Curve Chromatograms section of this report.
QC Notes All internal standard criteria were met in the analyses of these samples.
The sample 1R-ACB-M0010-02 was analyzed in duplicate and met all
duplicate criteria.
The analyses of the LCS and LCDS exhibited recovery values of 80-
120% for all reported compounds.
All sample preparation and analytical holding times specified in the
method were met.
Reporting Notes The results presented in this report are representative of the samples as
provided to the laboratory.
EA# 0111-20 Page 13 of 126
-------
General Reporting Notes
The following are general reporting notes that are applicable to all Enthalpy Analytical, Inc. data
reports, unless specifically noted otherwise.
• The acronym MDL represents the Minimum Detection Limit. Below this value the laboratory cannot
determine the presence of the analyte of interest reliably.
• The acronym LOQ represents the Limit of Quantification. Below this value the laboratory cannot
quantitate the analyte of interest within the criteria of the method.
• The acronym ND following a value indicates a non-detect or analytical result below the MDL.
• The letter / following a value indicates an analytical result between the MDL and the LOQ. A J flag
indicates that the laboratory can positively identify the analyte of interest as present, but the value
should be considered an estimate.
• The letter E following a value indicates an analytical result exceeding 100% of the highest calibration
point. The associated value should be considered as an estimate.
• The acronym DF represents Dilution Factor. This number represents dilution of the sample during the
preparation and/or analysis process. The analytical result taken from a laboratory instrument is
multiplied by the DF to determine the final undiluted sample results.
• The addition of MS to the Sample ID represents a Matrix Spike. An aliquot of an actual sample is
spiked with a known amount of analyte so that a percent recovery value can be determined. This shows
what effect the sample matrix may have on the target analyte, i.e. whether or not anything in the sample
matrix interferes with the analysis of the analyte(s).
• The addition of MSD to the Sample ID represents a Matrix Spike Duplicate. Prepared in the same
manner as an MS, the use of duplicate matrix spikes allows further confirmation of laboratory quality
by showing the consistency of results gained by performing the same steps multiple times.
• The addition of LD to the Sample ID represents a Laboratory Duplicate. The analyst prepares an
additional aliquot of sample for testing and the results of the duplicate analysis are compared to the
initial result. The result should have a difference value of within 10% of the initial result (if the results
of the original analysis are greater than the LOQ).
• The addition of AD to the Sample ID represents an Alternate Dilution. The analyst prepares an
additional aliquot at a different dilution factor (usually double the initial factor). This analysis helps
confirm that no additional compound is present and coeluting or sharing absorbance with the analyte of
interest, as they would have a different response/absorbance than the analyte of interest.
• The Sample ID LCS represents a Laboratory Control Sample. Clean matrix, similar to the client sample
matrix, prepared and analyzed by the laboratory using the same reagents, spiking standards and
procedures used for the client samples. The LCS is used to assess the control of the laboratory's
analytical system. Whenever spikes are prepared for our client projects, two extra spikes are prepared.
The extras (randomly chosen) are labeled with the associated project number and kept in-house at the
appropriate temperature conditions. When the project samples are received for analysis, the LCSs are
analyzed to confirm that the analyte could be recovered from the media, separate from the samples
which were used on the project and which may have been affected by source matrix, sample collection
and/or sample transport.
EA# 0111-20 Page 14 of 126
-------
General Reporting Notes
(continued)
Significant Figures: Where the reported value is much greater than unity (1.00) in the units expressed,
the number is rounded to a whole number of units, rather than to 3 significant figures. For example, a
value of 10,456.45 ug catch is rounded to 10,456 ug. There are five significant digits displayed, but no
confidence should be placed on more than two significant digits.
Manual Integration: The data systems used for processing will flag manually integrated peaks with
an "M". There are several reasons a peak may be manually integrated. These reasons will be identified
by the following two letter designations. The peak was not integrated by the software "NI", the peak
was integrated incorrectly by the software "II" or the wrong peak was integrated by the software
"WP". These codes will accompany the analyst's manual integration stamp placed next to the
compound name.
EA# 0111-20 Page 15 of 126
-------
Sample Custody
!
o
EA# 0111-20 Page 16 of 126
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
US EPA
RTF, NC
ACB Outlet Duct
RN99027 1.0002
Enthalpy
Sample ID
Sample Matrix
Analysis Requested
IR-ACB-MQOIO-O^.
Dry filter -^
Front Half MeOH/MeCl2 Rinse .
Impinger DI H2O ^^
Back Half MeOH/MeCl2 Rinse
XAD trap -^
Dry filter--^
Front Half MeOH/MeCl2 Rinse
Impinger DI H2O —'
Back Half MeOH/MeCl2 Rinse.
XAD trap ^s
SVOC
SVOC
SVOC
SVOC
SVOC
SVOC
SVOC
SVOC
SVOC
SVOC
Notes/Comments:
1
Field blank collected 2/9/11. Emission sample collected 2/16/11. Refrigerator temp. 38 deg. F.
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919)544-4535
Fax Number: (919)544-5690
EA# 0111-20 Page 17 of 126
-------
Sample
Chromatograms
(Scan)
EA# 0111-20 Page 18 of 126
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100171.D
21 Feb 2011 5:31 pm
tdd
0111-20 1R-ACB-M0010-02
14 Sample Multiplier: 1
Quant Time Feb 21 19:38:06 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
2.1e+07
2e+07
1.9e+07
1.8e+07
1.7e+07
1.6e+07
1.56+07
1.46+07
1.36+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
0
Time~> 3.00
TIC: W1100171.D\data.ms
I
O
4.00
5.00
6.00
7.00
9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 19:38:41 201lEA# 0111-20 Page 19 of 126
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100171.D
21 Feb 2011 5:31 pm
tdd
0111-20 1R-ACB-M0010-02
14 Sample Multiplier: 1
Quant Time Feb 21 19:38:06 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.247
.455
.212
. 724
. 502
.660
152
136
164
188
240
264
685096
2422513
1384296
2621296
2655817
2218030
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.131 112 160531
5) Phenol-d5 (surr) 4.863 99 217752
19) Nitrobenzene-d5 (surr) 5.744 82 229008
39) 2-Fluorobiphenyl (surr) 7.496 172 429091
57) Fluorene-dlO (surr) 8.730 176 312299
65) 2,4,6-Tribromophenol (... 9.008 330 61385
75) Pyrene-dlO (surr) 11.177 212 598696m
77) Terphenyl-dl4 (surr) 11.295 244 494933
Target Compounds
8.59 ug/mL
10.02 ug/mL
10.74 ug/mL
10.02 ug/mL
8.22 ug/mL
10.23 ug/mL
10.96 ug/mL
11.38 ug/mL
-0.02
0 . 00
0 . 00
-0 . 01
0 . 00
0.00
0.00
0.02
Qvalue
[Manual Int. "II"
3)
6)
7)
8)
9)
10)
11)
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2-Chlorophenol
1, 3-Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
12) Benzyl alcohol
13) 1,2-Dichlorobenzene
14) 2-Methylphenol
15) bis(2-Chloroisopropyl)...
16) 3/4-Methylphenol
17) N-Nitrso-di-n-propylam.
Hexachloroethane
Nitrobenzene
Isophorone
2, 4 -Dimethy^Upfienol
2-Nitropte£nol (CCC)
Benzole acid
(2-Chloroethoxy)met...
"2,4-Dichlorophenol (CCC)
u . u u u
3 . 180
4 . 873
0.000
0.000
0.000
0.000
0 . 000
0 . 000
0 . 000
5 .418
0.
"57
'0.000
0.000
0 . 000
0 . 000
6 . 075
6 . 070
6.161
0.000
0.000
u . u u u
n nrm
N.D.
59 .47
195 .34
N.D
N.D
3-3-)—1 Chloro 3 mothylphono . . .
N.D. d
33) 2-Methylnaphthalene
34) 1-Methylnaphthalene
7.149
7.256
n n n n
142
142
529527
410185
14.17 ug/mL
11.35 ug/mL
100
0.000
0 . 000
0 . 000
0 . 000
37) 2,4,6-Trichlorophenol
38) 2,4,5-Trichlorophenol
40) 2-Chloronaphthalene
41) 2-Nitroaniline
42) 1,4-Dinitrobenzene
43) Dimethylphthalate
44) 1,3-Dinitrc
45) 2,
4»f"T, 2 -Diiiiti-obe
W101810X-8270 .M Mon Feb 21 19:38:40 2011 EA# 0111-20 Page 20 of 126
N.D. d
0.000
0.000
0.000
0.000
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100171.D
21 Feb 2011 5:31 pm
tdd
0111-20 1R-ACB-M0010-02
14 Sample Multiplier: 1
Quant Time Feb 21 19:38:06 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
47) Acenaphthylene
4-8-)—3 Nitroanilinc
R.T. Qlon
;.073 152
Response
1597473
Cone Units Dev(Min)
28.69 ug/mL 94
49) Acenaphthene (CCC)
5-9-)—2,1 Dinitrophonol—fS
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-
55) 2,3,4,6-
56) DiethlwstetftaTate
1.40 ug/mL#
N.D. d
8.244 154
0.000
0 . 000
0 . 000
8.415 16
9.97 ug/mL#
N.D.
N.D.
2.28 ug/mL#
Tetrachlorophenol
0.000
8.565
9.18 ug/mL
N.D. d
8.762 166
0 . 000
0 . 000
0.000
0
0.000
0.000
N.D.
N.D.
N.D.
N.D.
Phenanthrene
70) Anthracene
-71) Par-V^^-lo
1296702
285592m
21.91 ug/mL
4.83 ug/mL
99-
_L
ig/Bl;
Manual Int. "WP" (Tt>t>)|
72) Di n butylphthalato
73) Fluoranthene (CCC)
7-4-)—Bonaidino
N.D. d
10.958
n n n n
202
639953
10.39 ug/mL
N.D. d
76) Pyrene
11.198 202
555852
7 9")Bu.Lylbt:iizy IpliLlidld L t:
80) 3,3-Dimethylbenzidine
81) bis(2-ethylhexyl)adipat^_
82) bis(2-Eth\
8.40 ug/mL#
u . u u u
0 . 000
149
0
48358
Q_
N.D. d
1.20 ug/mL#
95
84) Benzo(a)anthracene
85) Chrysene
H—&
_L ~ 11 ~ LJLJ
LyluhLhdlc
12.486 228 132322 2.06 ug/mL# 63
12.529 228 118034 1.95 ug/mL# 1
N.D. d
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
0.000
14.014
14.030
14.468
14.559
14.703
16.642
0.000
17.208
252
252
252
252
252
276
276
118581m
42266m
43800
76972
13088
40344
0
35945
2.14 ug/mL
0.78 ug/mL
0.89 ug/mL
1.51 ug/mL
N.D.
0.80 ug/mL
N.D. d
0.87 ug/mL
iManual Int. "II" (Tt>t^|
92
97
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 19:38:40 2011 EA# 0111-20 Page 21 of 126
Page: 2
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Ref 50
Scan 966 (8.755 min): W1000111 .D (-959) (-)
1 2
115
63
39 8
166193 231 281 327
387
m/z--> 50
Abundance
Raw
50
100 150
142
200 250 300 350
400
#33
2-Methylnaphthalene
Concen: 14.17 ug/mL
RT: 7.149 min Scan# 780
Delta R.T. 0.003 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
Tgt Ion:142 Resp: 529527
Ion Ratio Lower Upper
142 100
141 87.2 69.7 104.5
115
38
89
m/z-> 50
Abundance
100
142
Sub
50
115
0
39 63 89
166193
0 200
1 fifi 1 93
Abundance
250 281 4nnnnn
250 300 350 400
300000
200000
100000
9F11 981 n
7ft9
/
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m/z-> 50 100 150 200 250 300 350
Abundance Scan 984 (8.861 min): W1000111.D (-978) (-)
400 Time->
7.10 7.15 7.20
Ref 50
0
115
c,
63 RQ
°
#34
1-Methylnaphthalene
Concen: 11.35 ug/mL
RT: 7.256 min Scan# 800
Delta R.T. 0.010 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
166195221 271295327
m/z~> 50 100 150 200 250 300 350
Abundance
142
Raw 50
400 J-yi- Ion:142 Resp: 410185
Ion Ratio Lower Upper
142 100
141 89.0 72.5 108.7
115
m/z-->
Abundance
Sub 50
Abundance
400000
39 63 ag
166193220 256281 401
50 100 150 200 250 300 350 400 300000
142
200000
115 100000
39 63 89 A cc * QO oon 9RR /ini n
I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I
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I I I I I I
7f
56
I I I
m/z-->
50 100 150 200 250 300 350 400 Time-> 7.20
7.25 7.30
W1100171.D W101810X-8270.M
Mon
Page 7
-------
Abundance Scan 1125 (9.689 min): W1 0001 11. D (-1118) (-) #47
Ref 50
o
1;'2 Acenaphthylene
Concen: 28.6E
3 ug/mL
RT: 8.073 min Scan# 953
Delta R.T. -0
008 min
Lab File: W1100171.D
76 Acq: 21 Feb 2011 5:31 pm
50 j j 101126 177202 253 402
m/z~> 50 100 150 200 250 300 350 400 T9t Ion:152 Resp: 1597473
Abundance Ion Ratio Lower Upper
Raw 50
o
152 152 100
151 22 . 1 15 . 0 22 .4
153 14.2 10.2 15.2
Abundance
~7C
51 101126 177204 249280 325
m/z~> 50 100 150 200 250 300 350 400 1000000
Abundance
Sub
50
o
152
500000
~7C
50 101126 176204237 281 325 o
8.
j
m/z-> 50 100 150 200 250 300 350 400 Time-> 7.95 8.00 8.05
Abundance Scan 1154 (9.860 min): W1 0001 11 .D (-1 144) (-) #49
Ref 50
Q
fl)73
\^
8.10 8.15
1;3 Acenaphthene (CCC)
Concen: 1.40 ug/mL
RT: 8.244 min Scan# 985
Delta R.T. -0
005 min
?6 Lab File: W1100171.D
39 i 109 | Acq: 21 Feb 2011 5:31 pm
, i | |||, i, 1,30 1 184207 267 355
m/z-> 50 100 150 200 250 300 350 T9t I°n:154 Resp: 49789
Abundance Ion Ratio Lower Upper
Raw 50
0
153 154 100
183 153 0.0 85.4 128. 0#
39 65 152 74.3 41.4 62. 2#
91 121
Abundance
250000
207228251 281 341 000000
-i— r--|— i— i— r-i— |-r i i I I i i | I I I I | I I I I | I I I I | ZUUUUU
m/z~> 50 100 150 200 250 300 350
Abundance __ 150000
Sub
50
0
153
183
60 100000
OQ 1 O1
39 gl 1^1 50000
219 246267 o
/
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m/z~> 50 100 150 200 250 300 350 Time~> 8.22 8
8.244
r^""""
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24 8.26
W1100171.D W101810X-8270.M
Mon
Page
-------
Abundance
Scan 1243 (10.383 min): W1000111 .D (-1233) (-)
#59
Ref 50
o
1f>6 Fluorene
Concen: 9
RT: 8.762
Delta R.T.
Lab File:
82 -| 39 Acq: 21 Feb
I511,|II 11,3 j , 198 232 264 355 429
m/z~> 50 100 150 200 250 300 350 400 T9t Ion:166
Abundance Ion Ratio
Raw 50
o
1 66 166100
165 98.6
167 16.0
Abundance
39 82 113139 192220 262 327355 25000°
m/z~> 50 100 150 200 250 300 350 400 200000
Abundance
Sub
50
o
1 fifi
lbb 150000
100000
50000
50 113139 193220 262 327 o
A
/ \
m/z-> 50 100 150 200 250 300 350 400 Time-> 8.70
Abundance Scan 1412 (1 1.376 min): W1 0001 1 1.D (-1400) (-) #69
Ref 50
o
1 ''8 Phenanthrene
Concen: 21
RT: 9.745
Delta R.T.
Lab File:
76 152 i Acq: 21 Feb
50 : j ,98 126 j 200 227 267 303 327
m/z~> 50 100 150 200 250 300 T9t Ion:178
Abundance Ion Ratio
Raw 50
Q
178 178 100
179 16.1
176 18.9
Abundance
76 152
51 98 126 199219239260281
m/z~> 50 100 150 200 250 300 1000000
Abundance
Sub
50
Q
178
500000
76 152
50 'D 98 126 214235 268 o
.18 ug/mL
min Scan# 1082
0.003 min
W1100171 .D
2011 5:31 pm
Resp: 366177
Lower Upper
75 .4 113 . 0
10.9 16 .3
8.t62
\
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8.75 8.80
.91 ug/mL
min Scan# 1266
-0.005 min
W1100171.D
2011 5:31 pm
Resp: 1296702
Lower Upper
12.5 18.7
15.0 22.4
9.t45
J IA
> \^ \
m/z~> 50 100 150 200 250 300 Time~> 9.65 9.70 9.75 9.80
W1100171.D W101810X-8270.M
Mon
Page 10
-------
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Scan 1636 (12.692 min): W1000111 .D (-1622) (-)
#73
Ref 50
Q
2(12 Fluoranthene (CCC)
Concen: 10.39 ug/mL
RT: 10.958 min Scan# 1493
Delta R.T. 0 . Oil min
Lab File: W1100171.D
101 i Acq: 21 Feb 2011 5:31 pm
39 74, 128 17,4 233 267 327355 431
m/z-> ' 5I0II100II150II200II250II30I0II350II40I01"1 T9fc I°n:202 Resp: 639953
Abundance Ion Ratio Lower Upper
Raw 50
o
202 202 100
200 19.6 16 . 5 24 . 7
101 11 . 6 8.6 12 . 8
Abundance
43 73 101 600000
129 175 241 284 328357387415446
m/z-> 50 100 150 200 250 300 350 400
Abundance 400000
Sub 50
0
202
200000
41 73101129 174 241 284 328357387415446 Q
IQ/tlRR
/
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1 \.
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m/z-> 50 100 150 200 250 300 350 400 Time-> 10.90 10.95 11.00
Abundance Scan 1688 (12.997 min): W1 0001 1 1.D (-1672) (-) #76
Ref 50
Q
202 Pyrene
Concen: 8.40 ug/mL
RT: 11.198 min Scan# 1538
Delta R.T. 0.009 min
Lab File: W1100171.D
101 i Acq: 21 Feb 2011 5:31 pm
39 70 , ,, 128 , 17, 4 239267 310 355 446
m/z-> 50 100 150 200 250 300 350 400 T9fc Ion: 2 02 Resp: 555852
Abundance Ion Ratio Lower Upper
Raw 50
Q
202 202 100
203 23.8 14.5 21. 7#
88 6.4 3.4 5.0#
Abundance
1 01 500000
55 139 174 230258285 342 429
m/z~> 50 100 150 200 250 300 350 400 400000
Abundance
Sub
50
202 300000
200000
100000
101
AK 74 1-34 174 9-3O9RR 9QR T^Q AA7 n
11 198
7
/\ /
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,^_
m/z~>
50 100 150 200 250 300 350 400
Time~>
11.15 11.20 11.25
W1100171.D W101810X-8270.M
Mon
Page 12
-------
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Abundance
Scan 2023 (14.966 min): W1000111.D (-2016) (-)
Ref 50
0
Abundance
113
39 75, || 151 20°
282 342 446
50 100 150 200 250 300 350 400
228
Raw
50
m/z~>
Abundance
Sub
m/z~>
50
#85
Chrysene
Concen: 1.95 ug/mL
RT: 12.529 min Scan# 1787
Delta R.T. -0.010 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
Tgt Ion:228 Resp: 118034
Ion Ratio Lower Upper
228 100
226 186.8 23.6 35.4#
229 21.4 15.8 23.6
55
113
165 200
50 100 150 200 2!
228
55 83
113
Abundance
281 308 355385415 100000
300 350 400
50000
A 12.529
A A
'V
~p
150 200 256283310342 385415
50 100 150 200 250 300 350 400
Time~>
12.50
12.55
Abundance
Ref 50
Scan 2393 (17.140 min): W1000111.D (-2376) (-)
2$2
126
38 74
161 200 | 295 342 415
549
0
m/z~> 50 100 150 200 250 300 350 400 450 500 550
Abundance
252
Raw 50
#88
Benzo(b)fluoranthene
Concen: 2.14 ug/mL m
RT: 14.014 min Scan# 2065
Delta R.T. 0.005 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
Tgt Ion:252 Resp: 118581
Ion Ratio Lower Upper
252 100
253 31.0 17.9 26.9#
125 15.9 7.7 11.5#
207
73 126
39 163
m/z~> 50 100 150 200 250
Abundance
252
Sub
50
295 341 415 461
300 350 400 450 500
29439R 3RF1 41 5 4RQ
Abundance
50000
550 4000°
30000
20000
10000
n
T4.014
/ \
/ \
V
\
\
\
/ \
\
/ \
/ \
126
38 73 163 207
50 100 150 200 250 300 350 400 450 500 550 Time-> 13^5
14.00
14.05
W1100171.D W101810X-8270.M
Mon
Page 14
-------
Abundance Scan 2404 (1 7.204
Ref 50
o
min): W1 0001 11. D (-2398) (-) #89
2Jp Benzo (k) f luoranthene
Concen:
0.78 ug/mL m
RT: 14.030 min Scan#
126
50 83 i 1 57 200
m/z~> 50 100 150 200
Abundance
Raw 50
0
207
,M 73 126
41 166
m/z~> 50 100 150 200
Abundance
Sub 50
0
126
38 73 166 208
m/z~> 50 100 150 200
Abundance Scan 2502 (1 7.780
Ref 50
o
Delta R.T.
Lab File:
-0.026 min
W1100171 .D
2068
Acq: 21 Feb 2011 5:31 pm
, i 284 323355387 431 489
'250 "SOO "350 "400 "450 T9fc I°n:252 Resp: 42266
Ion Ratio
252 252 100
253 87.0
125 44.5
Abundance
50000
283 341 401 461
250 300 350 400 450 ' 4000°
252 30000
20000
10000
308 367401 461 0
\
\
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Lower Upper
14 . 9 22 .
8.8 13 .
3#
2#
14\030
250 300 350 400 450 Time->
min): W1 0001 11.D (-2489) (-) #90
"•~x
\
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14.05
2^2 Benzo ( e ) pyrene
Concen:
0.89 ug/mL
RT: 14.468 min Scan#
Delta R.T.
Lab File:
-0.014 min
W1100171.D
2150
125 Acq: 21 Feb 2011 5:31 pm
43 74 , j 174211
m/z~> 50 100 150 200
Abundance
Raw 50
207
55 125
91 165
oU-rT,-r-r-,-| .-v-r-rpr.-.T-T
m/z~> 50 100 150 200
Abundance
U 50
n
55 91 1^ 173207
, i 284 327 413446 489
'250 "SOO "350 "400 "450" 500 T9t I°n:252 Resp: 43800
Ion Ratio
252 252 100
250 30.0
253 25.3
Abundance
30000
| ^ ^ 283 J341 385417 497
250 300 350 400 450 500
20000
252
10000
286 336371 432 497 Q
Lower Upper
23.0 34.
17.8 26.
14.468
A
\
/
/ \ /
/ \ J
/ \ /
4
8
1
50 100 150 200 250 300 350 400 450 500 Time->
14.40 14.45 14.50
W1100171.D W101810X-8270.M
Mon
Page 15
-------
Abundance
Ref 50
Scan 2522 (17.897 min): W1000111 .D (-2511) (-)
2$2
126
48 86
174 211
295327 387 430
503
m/z~>
Abundance
Raw
50
50 100 150 200 250 300 350 400 450 500
252
207
#91
Benzo(a)pyrene (CCC)
Concen: 1.51 ug/mL
RT: 14.559 min Scan# 2167
Delta R.T. -0.018 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
Tgt Ion:252 Resp: 76972
Ion Ratio Lower Upper
252 100
253 24.4 16.6 25.0
0
m/z-->
Abundance
19R 40000]
73
41 163 283 325356389 429461
-T-r~l . r,T|-.-.-. -|-n r.-|-r r . , |-rr TJ i i i i | i i i i | i i i i | i i i i | i _nnnn
50 100 150 200 250 300 350 400 450 500 3000°
Abundance
Sub 50
0
m/z-->
Abundance
Ref 50
0
m/z-->
Abundance
252
20000
10000
126
40 75 159 198 295 344378 430 475 o
14&59
A
;
\ /
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50 100 150 200 250 300 350 400 450 500 Time-> 14.50 14.55 14.60
Scan 3015 (20.794 min): W10001 1 1.D (-2994) (-) #93
26 Indeno (1 , 2 , 3-cd) pyrene
Concen: 0.80 ug/mL
RT: 16.642 min Scan# 2557
138 Delta R.T. -0.068 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31
39 92,|| 191224 : 311343 415446 503
pm
50 100 150 200 250 300 350 400 450 500 T9t Ion:276 Resp: 40344
Ion Ratio Lower Upper
Raw 50
207
276 100
138 25.0 21.4
32.2
276
73 138
41 105 176
239
Abundance
Sub
50
276
138
207
37
75 176 245
m/z-> 50 100 150 200 250 300 350 400 450 500 Time->
Abundance
327 385 429461 15000
i i i I i i i i I i i i i I i i i i I
0 350 400 450 500
10000
5000
imAd 385/11 RAA7 /ISO n
16
ft42
I
/ V
16.60 16.70
W1100171.D W101810X-8270.M
Mon
Page 16
-------
Abundance
Ref 50
Scan 3157 (21.628 min): W1000111 .D (-3137) (-)
138
55 91
187222
323 368 416 489 549
#95
Benzo(g,h,i)perylene
Concen: 0.87 ug/mL
RT: 17.208 min Scan# 2663
Delta R.T. -0.085 min
Lab File: W1100171.D
Acq: 21 Feb 2011 5:31 pm
Abundance
Raw
50
50 100 150 200 250 300 350 400 450 500 550
207
276
Tgt Ion:276 Resp:
Ion Ratio Lower
276 100
138 26.7 21.2
277 23.7 19.3
m/z~>
73
138
39 173 241 313 355 415 451
OW-r] rr I I ] I n r | "I T n prrrTyT' I |-ryrT^i^i I I I |^^i I | i^^i I I I I |
50 100 150 200 250 300 350 400 450 500 550
Abundance
Sub
50
276
137
73
36 175209 313352 416 461
0 WT-I-T-I-I-TT T'T T-i-rr-n -q- rr^rrT-r [~T~rTT i~r^-| I I I ^^^^^'^ I I I I I
Abundance
10000
5000
17.208
35945
Upper
31. 8
28 . 9
m/z~>
50 100 150 200 250 300 350 400 450 500 550 Time-> 17.10 17.20 17.30
W1100171.D W101810X-8270.M
Mon
Page 17
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100172.D
21 Feb 2011 6:04 pm
tdd
0111-20 1R-ACB-M0010-02 LD
15 Sample Multiplier: 1
Quant Time Feb 21 19:10:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
2.5e+07
2.4e+07
2.3e+07
2.26+07
2.16+07
2e+07
1.96+07
1.86+07
1.76+07
1.66+07
1.56+07
1.46+07
1.36+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
TIC: W1100172.D\data.ms
Time~> 3.00
4.00
5.00
6.00
7.00
9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 19:11:48 201lEA# 0111-20 Page 33 of 126
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100172.D
21 Feb 2011 6:04 pm
tdd
0111-20 1R-ACB-M0010-02 LD
15 Sample Multiplier: 1
Quant Time Feb 21 19:10:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.247
.454
.212
. 724
. 502
.660
152
136
164
188
240
264
897371
3211606
1892982
3475619
3264792
2597182
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.131 112 209295
5) Phenol-d5 (surr) 4.868 99 272058
19) Nitrobenzene-d5 (surr) 5.744 82 287163
39) 2-Fluorobiphenyl (surr) 7.496 172 566923
57) Fluorene-dlO (surr) 8.730 176 413838
65) 2,4,6-Tribromophenol (... 9.008 330 81678
75) Pyrene-dlO (surr) 11.177 212 752659
77) Terphenyl-dl4 (surr) 11.295 244 621747
Target Compounds
8.55 ug/mL
9.56 ug/mL
10.28 ug/mL
9.69 ug/mL
7.96 ug/mL
10.26 ug/mL
10.39 ug/mL
10.78 ug/mL
-0.02
0 . 00
0 . 00
-0 . 01
0 . 00
0.00
0.00
0.02
Qvalue
3)
6)
7)
8)
9)
10)
11)
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2-Chlorophenol
1, 3-Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
12) Benzyl alcohol
13) 1,2-Dichlorobenzene
14) 2-Methylphenol
15) bis(2-Chloroisopropyl)...
16) 3/4-Methylphenol
17) N-Nitrso-di-n-propylam.
Hexachloroethane
Nitrobenzene
Isophorone
2, 4 -Dimethy^Upfienol
2-Nitropte£nol (CCC)
Benzole acid
(2-Chloroethoxy)met...
"2,4-Dichlorophenol (CCC)
u . u u u
3 .185
4 . 878
0.000
0.000
0.000
0.000
0 . 000
0 . 000
0 . 000
5 .418
0.
"57
'0.000
0.000
0 . 000
0 . 000
6 . 070
6 . 070
6.171
0.000
0.000
N.D.
57 . 84
188.02
N.D
N.D
u . u u u
n nrm
3-3-)—1 Chloro 3 mothylphono. . .
N.D. d
33) 2-Methylnaphthalene
34) 1-Methylnaphthalene
7.149
7.256
n n n n
142
142
690477
546423
13.94 ug/mL
11.41 ug/mL
100
0.000
0 . 000
0 . 000
0 . 000
37) 2,4,6-Trichlorophenol
38) 2,4,5-Trichlorophenol
40) 2-Chloronaphthalene
41) 2-Nitroaniline
42) 1,4-Dinitrobenzene
43) Dimethylphthalate
44) 1,3-Dinitrc
45) 2,
4»f"T, 2 -Diiiiti-obe
W101810X-8270 .M Mon Feb 21 19:11:47 2011 EA# 0111-20 Page 34 of 126
N.D. d
0.000
0.000
0.000
0.000
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100172.D
21 Feb 2011 6:04 pm
tdd
0111-20 1R-ACB-M0010-02 LD
15 Sample Multiplier: 1
Quant Time Feb 21 19:10:09 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
Response
2093917
Cone Units Dev(Min)
27.50 ug/mL# 82
Acenaphthylene
3 Mitroanilinc
1.29 ug/mL#
N . D . d
Acenaphthene
8.244 154
0.000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
8.415 16
9.86 ug/mL#
N.D.
N.D.
1.72 ug/mL#
Tetrachlorophenol
0.000
8.570
8.87 ug/mL#
N.D. d
Fluorene
i-Niti-oaiiiliiie
8.762 166
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D.
N.D.
N.D.
N.D.
21.50 ug/mL
4.74 ug/mL
Phenanthrene
Anthracene
Di n butylphthalato
9.86 ug/mL
N.D. d
Fluoranthene
7.88 ug/mL#
Bu.Lylbt:iizy IpliLlidld L
3 , 3-Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis(2-Eth
N.D. d
1.00 ug/mL#
1.97 ug/mL#
1.86 ug/mL#
N.D. d
84) Benzo(a)anthracene
85) Chrysene
12.486
12.529
155187
138276
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
14.008
14.035
14 .468
14.559
0.000
16.642
0.000
17.214
276
2.14 ug/mL
0.66 ug/mL
0.88 ug/mL
1.45 ug/mL
N.D. d
0.73 ug/mL
N.D. d
0.79 ug/mL
[Manual Int. "II"
90
97
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 19:11:47 2011 EA# 0111-20 Page 35 of 126
Page: 2
-------
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Abundance
Ref 50
Scan 966 (8.755 min): W1000111 .D (-959) (-)
142
115
39
63
89
168193 231 281 327
387
Abundance
Raw
50
50 100 150 200 250 300 350
142
400
#33
2-Methylnaphthalene
Concen: 13.94 y.g/mL
RT: 7.149 min Scan# 780
Delta R.T. 0.003 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:142 Resp: 690477
Ion Ratio Lower Upper
142 100
141 87.2 69.7 104.5
0
m/z-->
' I0 Abundance
600000
39 89 166191 219 267 299 341 401
50 100 150 200 250 300 350 400
Abundance 400000
Sub 50
0
m/z-->
142
200000
115
39 63 89 166192219 267 299 341 401 o
7
|
/
It
1
3
\
50 100 150 200 250 300 350 400 Time-> 7.10 7.15 7.20
Abundance Scan 984 (8.861 min): W1 0001 1 1.D (-978) (-) #34
Ref 50
0
m/z-->
Abundance
1'^2 1 -Methylnaphthalene
Concen: 11.41 ug/mL
RT: 7.256 min Scan# 800
Delta R.T. 0.
010 min
Lab File: W1100172.D
63 Acq: 21 Feb 2011 6:04 pm
3?,,|, , 1!3 195 240 295327
50 1 00 " 150 "200 "250 "SOO "350 "400" ' Tgt Ion: 142 Resp: 546423
Ion Ratio Lower Upper
Raw 50
Abundance
50
142
142 100
141 86.8 72.5 108.7
1 1 O
39 77
170201 239267297
50 100 150 200 250 300
142
41 77 105 172 207239 281
50 100 150 200 250 300
Abundance
500000
417446
400000
300000
200000
100000
417 o
iT
/
/ \
/ \
350 400 Time-> 7.20 7.25 7.30
W1100172.D W101810X-8270.M
Mon
Page 7
-------
Abundance Scan 1125 (9.689 min): W1 0001 11. D (-1118) (-) #47
Ref 50
Q
1;>2 Acenaphthylene
Concen: 27.50 ug/mL
RT: 8.073 min Scan# 953
Delta R.T. -0
.008 min
Lab File: W1100172.D
76 Acq: 21 Feb 2011 6:04 pm
50 | j 101126 177202 253 402
m/z-> ' 'SO ' ' 100 ' ' 150 ' ' 200 ' ' 250 ' ' 300 ' ' 350 ' ' 400 T9fc I°n:152 Resp: 2093917
Abundance Ion Ratio Lower Upper
Raw 50
0
152 152 100
151 31.0 15.0 22. 4#
153 13.9 10.2 15.2
Abundance
~7C
50 101126 176201229257281 341 -,Cnnnnn
—\ r |"~T "~r T ~r | i i i i "~i i i i i i i i i i i i i i i i i i i i r I OUUUUU
m/z-> 50 100 150 200 250 300 350 400
Abundance
Sub
50
o
152 1000000
500000
~7C
50 102126 178204229257282 0
8.
J
m/z-> 50 100 150 200 250 300 350 400 Time-> 8.00 8.05
Abundance Scan 1154 (9.860 min): W1 0001 11. D (-1144) (-) #49
Ref 50
o
fj>73
l_
8.10 8.15
1:3 Acenaphthene (CCC)
Concen: 1.29 ug/mL
RT: 8.244 min Scan# 985
Delta R.T. -0
.005 min
?6 Lab File: W1100172.D
39 i i 1°9 | Acq: 21 Feb 2011 6:04 pm
y 1 Hi. Li ,,, 184215 267 355
m/z-> 50 100 150 200 250 300 350 400 T9t Ion:154 Resp: 62647
Abundance Ion Ratio Lower Upper
Raw 50
0
60 153 154 100
153 657.9 85.4 128. 0#
183 152 81.5 41.4 62. 2#
Q1 121
Abundance
300000
210239 281310339 446
— |— i i i n r T | i i i i | i i i | i i i i | i i i i | i i i i | i i i i | i i i i |
m/z-> 50 100 150 200 250 300 350 400
Abundance 200000
Sub sn
OU
0
60 153
183
100000
121
91
;
21 1 240268 298 339 446 o
r~ ~| i i r i p i i T i r r T r | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i i
8.244
I
i i i i i | i i i
m/z-> 50 100 150 200 250 300 350 400 Time-> 8.20 8.22 8.24 8.26
W1100172.D W101810X-8270.M
Mon
Page
-------
Abundance Scan 1243 (10.383 min): W1 0001 1 1.D (-1233) (-) #59
Ref 50
n
1f
51 82 1131?9
,i. i, i, ! !iv J
6 Fluorene
Concen: 8
RT: 8.762
Delta R.T.
Lab File:
Acq: 21 Feb
19,8 232 264 355 429
U i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i .-ICC
m/z~> 50 100 150 200 250 300 350 400 L^ -Lon-J-oo
Abundance Ion Ratio
Raw 50
n
166 166 100
165 96.3
167 17.0
Abundance
oQ 82 , , „ 1 39
oa 113 194220249281 355
u i i | i i i i | i i i i | i i i | i i i i | i i i i | i i i i | i i i i | i i i i ^nnnnn
m/z-> 50 100 150 200 250 300 350 400 ouuuuu
Abundance
Sub 50
Q
166
200000
100000
82 x,,o139 /\
50 113
192218 266 355 Oi ''
m/z-> 50 100 150 200 250 300 350 400 Time-> 8.65 8.70
Abundance Scan 1412 (1 1.376 min): W1 0001 1 1.D (-1400) (-) #69
Ref 50
n
1
76
•30. ,i 1 1-a i
i J I
'8 Phenanthrene
Concen: 21
RT: 9.745
Delta R.T.
Lab File:
Acq: 21 Feb
207 267 303330
.87
min
ug/mL
Scan# 1082
0.003 min
W1100172 .D
2011 6:04 pm
Resp: 483637
Lower Upper
75
10
8.1
1
1
|
i
i
/
4 113.0
9 16. 3#
62
\
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8.75 8.80
.50
min
ug/mL
Scan# 1266
-0.005 min
W1100172.D
2011 6:04 pm
Abundance
Raw 50
Abundance
50 100 150 200 250 300 350 400
178
Tgt Ion:178 Resp: 1686639
Ion Ratio Lower Upper
178 100
179 16.1 12.5 18.7
176 18.8 15.0 22.4
Sub
50
m/z~>
Abundance
76 151 1500000
39 115 205233263 327355 446
50 100 150 200 250 300 350 400
1000000
1 78
500000
"7fi 1 m
39 111 207239268 327 446 o
50 100 150 200 250 300 350 400 Time->
9.;
/
9.65 9.70 9
r
k
75 9.80
W1100172.D W101810X-8270.M
Mon
Page 10
-------
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-------
Abundance
Ref 50
Scan 1636 (12.692 min): W1000111 .D (-1622) (-)
2C
101
39 74
128
14
233 267
327355
431
Abundance
Raw
50
50 100 150 200 250 300 350 400
202
m/z~>
Abundance
43 73 101 129
100
~'T'r
50
^ j\75 ^ 241 284311340 387
150 200 250 300 350 400
202
50
0
m/z~>
Abundance
Ref 50
#73
Fluoranthene (CCC)
Concen: 9.86 ug/mL
RT: 10.958 min Scan# 1493
Delta R.T. 0.Oil min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:202 Resp: 804904
Ion Ratio Lower Upper
202 100
200 19.7 16.5 24.7
101 11.9 8.6 12.8
Abundance
800000
600000
400000
200000
43 73101129
50
100
150
200
250 300 350 400
Time->
10.90 10.95 11.00
Scan 1688 (12.997 min): W1 0001 11. D (-1672) (-)
2(
101
39 70 ,
150
#76
Pyrene
Concen: 7.88 ug/mL
RT: 11.198 min Scan# 1538
Delta R.T. 0.009 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
239270 310 355
446
50 100 150
Abundance
Raw 50
200 250
202
300 350 400 450
Ion:202 Resp:
Ion Ratio Lower
202 100
203 23.9 14.5
88 5.9 3.4
691504
Upper
21.7#
5.0#
101
^41 70 ^ 131 1 °5 231260 295325355 401 446476
50 100 150 200 250 300 350 400 450
Abundance
Sub
50
202
Abundance
600000
400000
200000
63
150
231 290 331 370 420 474
98
m/z~>
50 100 150 200 250 300 350 400 450 Time->11.10 11.15 11.20 11.25
W1100172.D W101810X-8270.M
Mon
Page 12
-------
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a i> **
CO i-l r-\ \£>
^ H (U • •
o in a CM H
in a CM n
^o i— i ID
•• i-l [> 0
H a cu
H CQ ^ ^ H
O (DO H CM
CM Cti J
£! co
(U CM O CM 0
h CM -H • •
•• 4-) O ^ O
H d CC 0 CM
CM O Ctf H
H
tj1 4-> tl CO CT) IX>
D (J) O CM CM CM
fH, EH H CM CM CM
(3)
CM
CO
(3)
CM
OO
If)
CM
OO
Of
CO
CD
^
^"
* ^
''^ ^
CD"
(3)
CO
—
_
o
-ID
^~
-
O
O
^"
~
o
-ID
- CO
O
O
- CO
_
O
ID O
CM -
co ;
CM o
O o
CO- CO
i-^
CM 0
-un
CM
OO U
O l~ O
CM O
h CM
r
LO F °
\ — LO
"*" |- T-
o h
CM 1-
T- h O
r°
t ^~
CD h
CO i—
I LfJ
F
o
A
(Nl
1
rsi
O5
fi
G5-
o
s
^,_
O
CM
CO
I
X
o
co
o
Q
CM
O
O
-------
Abundance
Ref 50
Scan 2023 (14.966 min): W1000111.D (-2016) (-)
113
39 75 I I 146176
282 342
446
m/z~> 50 100 150 200 250 300 350 400 450
Abundance
228
Raw
50
0
55 113
84 152 189
n
281310341 383 429461
m/z-> 50 100 150 200 250 300 350 400 450
Abundance
228
Sub
m/z~>
50
43 83
113
151 198
284314 355 399430461
#85
Chrysene
Concen: 1.86 ug/mL
RT: 12.529 min Scan# 1787
Delta R.T. -0.010 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:228 Resp: 138276
Ion Ratio Lower Upper
228 100
226 192.7 23.6 35.4#
229 24 . 0 15.8 23.6#
Abi
100000
50000
12.529
50 100 150 200 250 300 350 400 450 Time->
12.50
12.55
Abundance
Ref 50
Scan 2393 (17.140 min): W1000111.D (-2376) (-)
2$2
126
38i 74 | I 161 200 [ 295 342 415
O(J i ~r | || I U I «-^w J, I ^J7O O'-til *-t I O 549
m/z~> 50 100 150 200 250 300 350 400 450 500 550
Abundance
252
Raw 50
#88
Benzo(b)fluoranthene
Concen: 2.14 ug/mL m
RT: 14.008 min Scan# 2064
Delta R.T. -0.000 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:252 Resp: 138735
Ion Ratio Lower Upper
252 100
253 32.7 17.9 26.9#
125 15.0 7.7 11.5#
207
Abui
39
73
126
163
n'
T
289328363401 446
Tp-rr-rjT
m/z~> 50 100 150 200 250 300 350 400 450 500 550
Abundance
252
Sub
50
40000
20000
126
39 79 162 207
50 100 150 200 250 300 350 400 450 500 550 Time->
314350 403 446
13.95
14.00
14.05
W1100172.D W101810X-8270.M
Mon
Page 14
-------
Abundance Scan 2404 (17.204 min): W1 0001 1 1.D (-2398) (-) #89
Ref 50
o
2;>2 Benzo (k) f luoranthene
Concen:
0.66 ug/mL m
RT: 14.035 min Scan#
Delta R.T.
Lab File:
-0.021 min
W1100172 .D
1 Acq: 21 Feb 2011 6:04
50 83 j 157 200 t , 284323355387 431 489
2069
pm
m/z~> 50 100 150 200 250 300 350 400 450 T9t Ion:252 Resp: 41874
Abundance Ion Ratio
Raw 50
0
252 252 100
207 253 108.2
125 50.1
Abur$t5bt5v
73 126
165 283 31 5 355 415 462
1 ' ~| I 1 1 I | 1 I 1 I | 1 1 I 1 I 1 I I I 1 I 1 | T T 1 1 ~| T"l 1 1 | 1 1 1 1 | 1 1 1 1 | 1
m/z-> 50 100 150 200 250 300 350 400 450 .........
Abundance 4000°
Sub
50
0
252
20000
126
38 73 165198 284320355 401 475 o
\
\
\
\
Lower Upper
14 . 9 22 .
8.8 13 .
3#
2#
14\D35
\
\
\
^^
m/z~>
50 100 150 200 250 300 350 400 450
Time~>
14.05
14.10
Abundance
Ref 50
Scan 2502 (17.780 min): W1000111.D (-2489) (-)
252
125
43 74
174 222 j | 282 327
413446 489
0
m/z~> 50 100 150 200 250 300 350 400 450
Abundance
207 252
Raw 50
43 73 125
177 282312 355 399429 469
m/z~> 50 100 150 200 250 300 350 400 450
Abundance
252
#90
Benzo(e)pyrene
Concen: 0.88 ug/mL
RT: 14.468 min Scan# 2150
Delta R.T. -0.014 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:252 Resp: 50794
Ion Ratio Lower Upper
252 100
250 28.2 23.0 34.4
253 22.7 17.8 26.8
Abundance
Sub
50
55
""T
125
95 160 207
286319 356388419 477
40000
30000
20000
10000
r\
1 4.468
A
A
1 \
\
/ \ /
/ \ J
50 100 150 200 250 300 350 400 450
Time~> 14.40 14.45 14.50
W1100172.D W101810X-8270.M
Mon
Page 15
-------
Abundance
Ref 50
Scan 2522 (17.897 min): W1000111 .D (-2511) (-)
2$2
126
48 86 I 174 211,
295327 387 430
503
m/z~> 50 100 150 200 250 300 350 400 450 500
Abundance
252
Raw
50
#91
Benzo(a)pyrene (CCC)
Concen: 1.45 ug/mL
RT: 14.559 min Scan# 2167
Delta R.T. -0.018 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:252 Resp: 86534
Ion Ratio Lower Upper
252 100
253 24.0 16.6 25.0
207
0
m/z~>
Abundance
Sub 50
0
m/z~>
Abundance
Ref 50
n
Abundance
73 126
41 163 300 341372 429461 40000
50 100 150 200 250 300 350 400 450 500
30000
252
20000
10000
126
42 73 166198 300332 370 430 476 o
14*59
I
\
\
U V /-
50 100 150 200 250 300 350 400 450 500 Time-> 14.50 14.55 14.60 14.65
Scan 3015 (20.794 min): W10001 1 1.D (-2994) (-) #93
2/6 Indeno (1 , 2 , 3-cd) pyrene
Concen: 0.73 ug/mL
RT: 16.642 min Scan# 2557
138 Delta R.T. -0.068 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
39 92,| 191224 , 311343 415446 503
m/z~> 50 100 150 200 250 300 350 400 450 500
Abundance
207
Raw 50
Tgt Ion:276 Resp: 43017
Ion Ratio Lower Upper
276 100
138 21.7 21.4 32.2
276
Abundance
73
41
133
176 238 313 355 403 446 489
m/z~> 50 100 150 200 250 300 350 400 450 500
Abundance
276
Sub
50
15000
10000
5000
138
463
51 89 172 2Q5 237 320357 404
m/z-> 50 100 150 200 250 300 350 400 450 500 Time->
16/642
16.60
16.70
W1100172.D W101810X-8270.M
Mon
Page 16
-------
Abundance
Scan 3157 (21.628 min): W1000111 .D (-3137) (-)
Ref 50
0
m/z-->
Abundance
2'
138
55 91 ,,
187222 ,
'6
323 368 416 489 549
50 100 150 200 250 300 350 400 450 500 550
Raw
50
207
#95
Benzo(g,h,i)perylene
Concen: 0.79 ug/mL
RT: 17.214 min Scan# 2664
Delta R.T. -0.080 min
Lab File: W1100172.D
Acq: 21 Feb 2011 6:04 pm
Tgt Ion:276 Resp: 38236
Ion Ratio Lower Upper
276 100
138 23 . 8 21.2 31.8
277 23.6 19.3 28.9
276
Abundance
73
39
133
167 241 313355399435475
m/z~>
Abundance
Sub
m/z~>
50
50 100 150 200 250 300 350 400 450 500 550
276
10000
5000
138
41
81
177 225 313 355 399 446 489
17A14
50 100 150 200 250 300 350 400 450 500 550 Time-> 17.10
17.20
17.30
W1100172.D W101810X-8270.M
Mon
Page 17
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100167.D
21 Feb 2011 3:08 pm
tdd
0111-20 ACB-M0010-FB
9 Sample Multiplier: 1
Quant Time Feb 21 16:24:56 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
0
TIC: W1100167.D\data.ms
Cfl
"b"
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 16:25:04 201lEA# 0111-20 Page 47 of 126 Page: 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\febll\W022111A\
W1100167.D
21 Feb 2011 3:08 pm
tdd
0111-20 ACB-M0010-FB
9 Sample Multiplier: 1
(QT Reviewed)
Quant Time Feb 21 16:24:56 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.242
.454
.212
. 719
.496
.655
152
136
164
188
240
264
725663
2519480
1421101
2538416
2681283
2484829
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.114 112 300841
5) Phenol-d5 (surr) 4.841 99 346781
19) Nitrobenzene-d5 (surr) 5.744 82 353511
39) 2-Fluorobiphenyl (surr) 7.496 172 825474
57) Fluorene-dlO (surr) 8.730 176 614909
65) 2,4,6-Tribromophenol (... 9.003 330 91293
75) Pyrene-dlO (surr) 11.177 212 1152403
77) Terphenyl-dl4 (surr) 11.294 244 918194
Target Compounds
15.21 ug/mL
15.07 ug/mL
15.65 ug/mL
18.79 ug/mL
15.76 ug/mL
15.71 ug/mL
21.78 ug/mL
21.80 ug/mL
-0.03
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.02
0.00
0.02
Qvalue
N.D.
N.D.
N.D.
N.D.
N.D^
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
1.48 ug/mL
N.D. d
N.D. d
Pyridine 0.000
Phenol (CCC) 0.000
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 0.000
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 0.000
Benzpifcacid 6.075
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
d
105
,4-Tliuhll
Naphthalene
N.D.
N.D. d
N.D. d
u . u u u
0 . 000
4-Chlun
TJovan>-i1 ,
u . u u u
n nrm
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D.
2-Methylnaphthalene
1-Methylnaphthalene
0.000
0.000
n n n n
N.D.
N.D.
0.000
0 . 000
0 . 000
0 . 000
0.000
0.000
0.000
0.000
2 , 4 , 6-Trichlorophenol
2 , 4 , 5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
" , 2 -Diiiiti-obe
W101810X-8270 .M Mon Feb 21 16:25:03 2011 EA# 0111-20 Page 48 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100167.D
21 Feb 2011 3:08 pm
tdd
0111-20 ACB-M0010-FB
9 Sample Multiplier: 1
Quant Time Feb 21 16:24:56 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon
0.000
Cone Units Dev(Min)
N.D.
Acenaphthylene
3 Nitroanilinc
Acenaphthene
0 . 000
0 . 000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
0.000
N.D.
N.D.
N.D.
1.82 ug/mL
Tetrachlorophenol
0.000
8.565
Fluorene
i-Niti-oaiiiliiie
0 . 000
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D
N.D
N.D
N.D
Phenanthrene
Anthracene
7*4-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
10.210
15023
N.D.
0.000
o
N.D.
N.D.
Pyrene
0.000
N.D. d
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipat^
bis(2-Eth ~~
L _L . / ^± J5 _L^± ^
0 . 000
0
28209
N.D. d
0.69 ug/mL#
99
84) Benzo(a)anthracene
85) Chrysene
0.000
0.000
N.D.
N.D.
d
d
i) DJ
jLyluhLlidldLb
0.000
0 . 000
0 . 000
0 . 000
0 . 000
0.000
0.000
0.000
0.000
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 16:25:03 2011 EA# 0111-20 Page 49 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100166.D
21 Feb 2011 2:36 pm
tdd
0111-20 MB
8 Sample Multiplier: 1
Quant Time Feb 21 16:12:32 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1100166.D\data.ms
Cfl
"b"
I
O
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 16:12:39 201lEA# 0111-20 Page 50 of 126 Page: 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\febll\W022111A\
W1100166.D
21 Feb 2011 2:36 pm
tdd
0111-20 MB
8 Sample Multiplier: 1
(QT Reviewed)
Quant Time Feb 21 16:12:32 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.242
.454
.207
. 719
.497
.660
152
136
164
188
240
264
670592
2325282
1339713
2297128
2342775
2147243
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.115 112 250857
5) Phenol-d5 (surr) 4.841 99 308078
19) Nitrobenzene-d5 (surr) 5.744 82 275269
39) 2-Fluorobiphenyl (surr) 7.496 172 679603
57) Fluorene-dlO (surr) 8.730 176 540343
65) 2,4,6-Tribromophenol (... 9.003 330 81848
75) Pyrene-dlO (surr) 11.177 212 991551
77) Terphenyl-dl4 (surr) 11.295 244 770215
Target Compounds
13.72 ug/mL
14.49 ug/mL
13.19 ug/mL
16.41 ug/mL
14.69 ug/mL
15.56 ug/mL
20.71 ug/mL
20.21 ug/mL
-0.03
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.02
0.00
0.02
Qvalue
N.D.
N.D.
N.D.
N.D.
N.D^
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
2.25 ug/mL
N.D. d
N.D. d
Pyridine 0.000
Phenol (CCC) 0.000
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 0.000
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 0.000
Benzpifcacid 6.081
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
d
105
,4-Tliuhll
Naphthalene
N.D.
N.D. d
N.D. d
u . u u u
0 . 000
4-Chlun
TJovan>-i1 ,
u . u u u
n nrm
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D.
2-Methylnaphthalene
1-Methylnaphthalene
0.000
0.000
n n n n
N.D.
N.D.
0.000
0 . 000
0 . 000
0 . 000
0.000
0.000
0.000
0.000
2 , 4 , 6-Trichlorophenol
2 , 4 , 5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
, 2 -Diiiiti-obe
W101810X-8270 .M Mon Feb 21 16:12:38 2011 EA# 0111-20 Page 51 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100166.D
21 Feb 2011 2:36 pm
tdd
0111-20 MB
8 Sample Multiplier: 1
Quant Time Feb 21 16:12:32 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon
0.000
Cone Units Dev(Min)
N.D.
Acenaphthylene
3 Nitroanilinc
Acenaphthene
0 . 000
0 . 000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
0.000
N.D.
N.D.
N.D.
2.10 ug/mL
Tetrachlorophenol
0.000
8.565
Fluorene
i-Niti-oaiiiliiie
0 . 000
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D
N.D
N.D
N.D
Phenanthrene
Anthracene
0 . 000
0 . 000
7*4-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
N.D. d
0.000
N.D.
N.D.
Pyrene
0.000
N.D. d
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipate_
bis(2-Eth\
0 . 000
12.347 149
n nnn
0
20413
Q_
N.D. d
0.58 ug/mL#
84) Benzo(a)anthracene
85) Chrysene
0.000
0.000
N.D.
N.D.
d
d
LyluhLhdldL
0.000
0 . 000
0 . 000
0 . 000
0 . 000
0.000
0.000
0.000
0.000
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
99
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) Indeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 16:12:38 2011 EA# 0111-20 Page 52 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100164.D
21 Feb 2011 1:30 pm
tdd
CH2C12 System Blank
7 Sample Multiplier: 1
Quant Time Feb 21 14:13:03 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1100164.D\data.ms
>.
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 14:13:56 201lEA# 0111-20 Page 53 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100164.D
21 Feb 2011 1:30 pm
tdd
CH2C12 System Blank
7 Sample Multiplier: 1
Quant Time Feb 21 14:13:03 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1) 1,4-Dichlorobenzene-d4... 5.242
21) Naphthalene-d8 (I) 6.455
36) Acenaphthene-dlO (I) 8.212
64) Phenanthrene-dlO (I) 9.719
78) Chrysene-dl2 (I) 12.497
87) Perylene-dl2 (I) 14.655
System Monitoring Compounds
4) 2-Fluorophenol (surr) 0.000
5) Phenol-d5 (surr) 0.000
19) Nitrobenzene-d5 (surr) 0.000
39) 2-Fluorobiphenyl (surr) 0.000
57) Fluorene-dlO (surr) 0.000
65) 2,4,6-Tribromophenol (... 0.000
75) Pyrene-dlO (surr) 0.000
77) Terphenyl-dl4 (surr) 0.000
Target Compounds
152 723138
136 2495204
164 1439095
188 2532591
240 2447876
264 2164661
112
99
82
172
176
330
212
244
40.00 ug/mL
40.00 ug/mL
40.00 ug/mL
40.00 ug/mL
40.00 ug/mL
40.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00 ug/mL
0.00
0.00
0.00
0.00
0.00
0.00
Qvalue
N.D.
N.D.
N.D.
N.D.
N.D.^
Pyridine 0.000
Phenol (CCC) 0.000
Aniline 0.000
bis(2-Chloroethyl)ether 0.000
2-Chlorophenol 0.000
1,3-Dichlorobenzene 0.000
1,4-Dichlorobenzene (CCC) 0.000
Benzyl alcohol 0.000
1,2-Dichlorobenzene 0.000
2-Methylphenol 0.000
bis(2-Chloroisopropyl)... 0.001
3/4-Methylphenol
N-Nitrso-di-n-propylam. . .^^0.000
Hexachloroethane ^s^ 0.000
Nitrobenzene ^s^ 0.000
Isophorone ^s^ 0.000
2 , 4-Dimethy^Uprnenol 0.000
2-Nitropte£nol (CCC) 0.000
Benzpifcacid 0.000
(2-Chloroethoxy)met... 0.000
"2,4-Dichlorophenol (CCC) 0.000
,4-Tliuhll
Naphthalene
4-Chlun
TJovan>-i1 ,
N.D. d
N.D. d
u . u u u
n nrm
3-^-
33)
34)
3-i4-
37)
38)
40)
41)
42)
43)
44)
45)
1 Chloro 3 mothylphono. . .
N.D.
2-Methylnaphthalene
1-Methylnaphthalene
0.000
0.000
n n n n
N.D.
N.D.
0.000
0 . 000
0 . 000
0 . 000
0.000
0.000
0.000
0.000
2 , 4 , 6-Trichlorophenol
2 , 4 , 5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
2,
" , 2 -Diiiiti-obe
W101810X-8270 .M Mon Feb 21 14:13:55 2011 EA# 0111-20 Page 54 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100164.D
21 Feb 2011 1:30 pm
tdd
CH2C12 System Blank
7 Sample Multiplier: 1
Quant Time Feb 21 14:13:03 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon
0.000
Cone Units Dev(Min)
N.D.
Acenaphthylene
3 Nitroanilinc
Acenaphthene
0 . 000
0 . 000
4-Nitrophenol (SPCC)
2 , 4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
DiethlwstetftaTate
0 . 000
0 . 000
0.000
N.D.
N.D.
N.D.
1.99 ug/mL
Tetrachlorophenol
0.000
8.565
Fluorene
i-Niti-oaiiiliiie
0 . 000
0 . 000
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
0 . 000
0.000
0
0.000
0.000
N.D
N.D
N.D
N.D
Phenanthrene
Anthracene
0 . 000
0 . 000
7*4-
73)
744-
76)
79T
80)
81)
82)
Di n butylphthalato
Fluoranthene (CCC)
Bonaidino
N.D. d
0.000
N.D.
N.D.
84)
85)
Pyrene
0.000
N.D.
Bu.Lylbt:iizy IpliLlidld L t:
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipate_
bis(2-Eth\
u . u u u
0 . 000
Benzo (a) anthracene
Chrysene
0.000
0.000
ILyX^jliLI id. _LciLc (CCC) 0 . 000
88) Benzo(b)fluoranthene 0.000
89) Benzo(k)fluoranthene 0.000
90) Benzo(e)pyrene 0.000
91) Benzo(a)pyrene (CCC) 0.000
92) Perylene 0.000
93) Indeno(1,2,3-cd)pyrene 0.000
94) Dibenz(a,h)anthracene 0.000
95) Benzo(g,h,i)perylene 0.000
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 14:13:55 2011 EA# 0111-20 Page 55 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100158.D
21 Feb 2011 10:12 am
tdd
0111-20 LCS
3 Sample Multiplier: 1
Quant Time Feb 21 10:34:53 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1100158.D\data.ms
c>
U!
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 10:35:15 201lEA# 0111-20 Page 56 of 126 Page: 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\febll\W022111A\
W1100158.D
21 Feb 2011 10:12 am
tdd
0111-20 LCS
3 Sample Multiplier: 1
(QT Reviewed)
Quant Time Feb 21 10:34:53 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.247
.454
.212
. 724
. 502
.660
152
136
164
188
240
264
807525
2791269
1650724
2996877
3059085
2714151
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.115
5) Phenol-d5 (surr) 4.841
19) Nitrobenzene-d5 (surr) 5.744
39) 2-Fluorobiphenyl (surr) 7.496
57) Fluorene-dlO (surr) 8.730
65) 2,4,6-Tribromophenol (... 9.003
75) Pyrene-dlO (surr) 11.177
77) Terphenyl-dl4 (surr) 11.295
Target Compounds
112 323591
99 407923
82 427798
172 974679
176 779205
330 135784
212 1363687
244 1114833
14.70 ug/mL
15.93 ug/mL
17.02 ug/mL
19.10 ug/mL
17.19 ug/mL
19.79 ug/mL
21.83 ug/mL
22.42 ug/mL
-0.03
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.02
0.00
0.02
Qvalue
N-NiLiubJudimeLhylamiiib! 3 .121
Pyridine 3.180
Phenol (CCC) 4.857
Aniline 4.937
bis(2-Chloroethyl)ether 4.959
2-Chlorophenol 5.055
1,3-Dichlorobenzene 5.199
1,4-Dichlorobenzene (CCC) 5.263
Benzyl alcohol 5.333
1,2-Dichlorobenzene 5.407
2-Methylphenol 5.407
bis(2-Chloroisopropyl)... 5.44J
3/4-Methylphenol 5x^€l
N-Nitrso-di-n-propylam. .^^^^.562
Hexachloroethane ^s^ 5.733
Nitrobenzene .s^ 5.760
Isophorone ^s^ 5.974
2 , 4-Dimethy^Uprnenol 6.054
2-Nitropte£nol (CCC) 6.064
Benzpifcacid 6.080
(2-Chloroethoxy)met... 6.145
"2,4-Dichlorophenol (CCC) 6.289
212132
301847
412030
115994
215699
374547
422216
44783;
22X95
fl3122
292170
264982
371717
240360
151702
362549
663582
390649
217248
73977m
381714
323422
13 . 64
11 . 67
15 .41
9.59
13.66
^
,.26
15 . 95
17 . 18
15 . 66
16 . 51
13.58
16.05
14.89
14.79
15 . 64
16 .28
19 .43
17 . 03
5.47
15.38
18.17
,4-Tliuhll
29) Naphthalene
ug/mL#
ug/mL#_
ugA
^g?r
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
y.g/mL
y.g/mL
y.g/mL
y.g/mL
ug/mL
y.g/mL
rr
128
|j.y / LLIJ_I
y.g/mL
100
4-Chlun
TJovantLL
M-y/
LLlL
r /ml.
Chloro 3 mothylphonc
~1 "1 O O ~1 ,
2-Methylnaphthalene
1-Methylnaphthalene
780660
756707
10.30 ug/mL
18.13
18.17
1 ~1 Q A
y.g/mL
ug/mL
7.416
7 .453
7 . 646
7 . 721
252784
256067
7578
751
128060
921909
147546
216372
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
7.870
7.918
7.945
0.014
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
., 2-Diiiifei.-eibi!
W101810X-8270 .M Mon Feb 21 10:35:14 2011 EA# 0111-20 Page 57 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100158.D
21 Feb 2011 10:12 am
tdd
0111-20 LCS
3 Sample Multiplier: 1
Quant Time Feb 21 10:34:53 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon
Response
1228078
Cone Units Dev(Min)
Acenaphthylene
3 Mitroanilinc
8.073
0.12
ug/mL
ug/mLtt
Acenaphthene
795545
01102
4-Nitrophenol (SPCC)
2,4-Dinitrotoluene
Dibenzofuran
2,3,5,6-
2,3,4,6-
D i e t h Lsas^rtrttan a t e
8 .255
8 .356
8.415
142556
283620
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
/ml.
18.80
17.30
17.71
24.50
Tetrachlorophenql
217453
225273
1181375
8.527
8.570
Q H~> n
Fluorene
-NitL-oaiiiliiie
894472
10000
18 . 81
14 .30
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine...
Azobenzene
4-Bromophenyl-ph
8 . 778
8.842
ug/mL
ug/mL#
ug/mL
ug/mL
760554
284824
306727
17.60
18.14
18.84
9.232
9.334
ug/mL
ug/mL
Phenanthrene
Anthracene
r?^~\ o
9.745 178
9.799 178
Q QT H 1 £"7
19 . 63
19 .37
Di n butylphthalato
Fluoranthene
Bu.Lylbfc^nzy IpliLlidld
. y J-JJllLlld-LdLt:T
88) Benzo(b)fluoranthene 14.008 252 1307149 19.26
89) Benzo(k)fluoranthene 14.046 252 1346723 20.31
90) Benzo(e)pyrene 14.473 252 1295478 21.43
91) Benzo(a)pyrene (CCC) 14.564 252 1262471 20.23
92) Perylene 14.703 252 1324734 20.34
93) Indeno(1,2,3-cd)pyrene 16.653 276 1264804 20.49
94) Dibenz(a,h)anthracene 16.658 278 1052808 20.22
95) Benzo(g,h,i)perylene 17.219 276 1021882 20.30
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
99
94
99
98
100
97
98
97
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 10:35:14 2011 EA# 0111-20 Page 58 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100160.D
21 Feb 2011 11:16 am
tdd
0111-20 LCSD
Reprep
4 Sample Multiplier: 1
Quant Time Feb 21 12:56:18 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1100160.D\data.ms
>.
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Feb 21 12:57:06 201lEA# 0111-20 Page 59 of 126 Page: 3
-------
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
Quantitation Report
M:\ms2011ql\wiley\data\febll\W022111A\
W1100160.D
21 Feb 2011 11:16 am
tdd
0111-20 LCSD
Reprep
4 Sample Multiplier: 1
(QT Reviewed)
Quant Time Feb 21 12:56:18 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . .
Naphthalene-dS (I)
Acenaphthene-dlO (
Phenanthrene-dlO (
Chrysene-dl2 (I)
Perylene-dl2 (I)
I)
I)
5.
6 .
8 .
9.
12 .
14.
.247
.454
.212
. 724
.497
.660
152
136
164
188
240
264
730272
2523281
1479378
2632375
2529691
2231292
40.
40 .
40 .
40 .
40 .
40.
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.
0 .
0 .
0 .
0 .
0.
.00
. 00
. 00
. 00
. 00
.00
System Monitoring Compounds
4) 2-Fluorophenol (surr) 4.115
5) Phenol-d5 (surr) 4.841
19) Nitrobenzene-d5 (surr) 5.744
39) 2-Fluorobiphenyl (surr) 7.496
57) Fluorene-dlO (surr) 8.730
65) 2,4,6-Tribromophenol (... 9.003
75) Pyrene-dlO (surr) 11.177
77) Terphenyl-dl4 (surr) 11.295
Target Compounds
112 266783
99 355702
82 371886
172 866000
176 710080
330 112897
212 1125850
244 911997
13.40 ug/mL
15.36 ug/mL
16.36 ug/mL
18.93 ug/mL
17.48 ug/mL
18.73 ug/mL
20.52 ug/mL
20.88 ug/mL
-0.03
-0 . 02
0 . 00
-0 . 01
0 . 00
-0.02
0.00
0.02
Qvalue
N-NiLiubJudimeLhylamiiib! 3 .121
Pyridine 3.180
Phenol (CCC) 4.857
Aniline 4.937
bis(2-Chloroethyl)ether 4.959
2-Chlorophenol 5.055
1,3-Dichlorobenzene 5.199
1,4-Dichlorobenzene (CCC) 5.263
Benzyl alcohol 5.333
1,2-Dichlorobenzene 5.407
2-Methylphenol 5.407
bis(2-Chloroisopropyl)... 5.44J
3/4-Methylphenol 5x^€l
N-Nitrso-di-n-propylam. .^^^^.562
Hexachloroethane ^s^ 5.733
Nitrobenzene .s^ 5.760
Isophorone ^s^ 5.974
2 , 4-Dimethy^Uprnenol 6.054
2-Nitropte£nol (CCC) 6.064
Benzpifcacid 6.080
(2-Chloroethoxy)met... 6.145
"2,4-Dichlorophenol (CCC) 6.289
,4-Tliuhll
29) Naphthalene
1 o 9 2 4 o
280180
358928
111423
187469
316085
342314
35560;
19>«*3;
544926
256878
229072
337362
218202
125242
316349
588161
347980
189068
54968m
333765
286577
331751—
12 . 04
11 . 98
14 . 85
10.19
13.13
14
^f
14 . 01
16 .29
14 .45
16 . 05
12.98
16.10
14.95
13.50
15 . 09
15 . 97
19 . 14
16 .40
4.50
14.88
17.81
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
W
128 1019040
|j.y / LLIJ_I
ug/mL
4-Chlun
TJovan>-i1 ,
o o / u :?
i en a AO
M-y/
LLlL
r /ml.
mothylphono. . .
-i^
p n /r p 1 ^
2-Methylnaphthalene
1-Methylnaphthalene
699687
681128
1 C. H 1 Q Q
17.98
18.10
1 O Q £
ug/mL
ug/mL
y.g/mL
7.416
7 .453
7 . 646
7 . 721
225735
230508
6747
973
112969
821692
129926
191376
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
lig/mL#
7.870
7.918
7.945
8-r-e
2,4,6-Trichlorophenol
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrc
., 2-Diiiifei.-eibi!
W101810X-8270 .M Mon Feb 21 12:57:05 2011 EA# 0111-20 Page 60 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100160.D
21 Feb 2011 11:16 am
tdd
0111-20 LCSD
Reprep
4 Sample Multiplier: 1
Quant Time Feb 21 12:56:18 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon
Response
1105850
Cone Units Dev(Min)
47) Acenaphthylene
4-8-)—3 Mitroanilinc
8.073
0.127
ug/mL
ug/mLtt
18 .27
12 . 01
.255
8 .356
8.415
119166
250433
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
18.82
17.03
17.44
22.01
Tetrachlorophenol
191888
198876
951200
59) Fluorene
66-)—4-NitL-oaiiiliiie
809596
131334
19 . 00
13 . 04
61) 4,6-Dinitro2-methylphenol
62) N-Nitrosodiphenylamine...
63) Azobenzene
66) 4-Bromophenyl-ph
8 . 778
8.842
ug/mL
ug/mL#
ug/mL
ug/mL
700713
258220
272153
9.227
9.334
ug/mL
ug/mL
Phenanthrene
70) Anthracene
2) Di n butylphthalat
73) Fluoranthene (CCC)
76) Pyrene
11.198 202
49) Acenaphthene (CCC)
5-9-)—2,1 Dinitrophonol—fS
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-
55) 2,3,4,6-
56) DiethlwstetftaTate
7 9")Bu.Lylbt:iizy IpliLlidld L
80)
81)
82)
834-
84)
85)
88)
89)
90)
91)
92)
93)
94)
95)
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethvl 1ir—/1 ^ jjhl h i "
~l n i _ | 'i -{ r-i'h 1 ^^-K-^^V-iQ-n <-? -1 H -i T-I c=±
Benzo (a) anthracene
Chrysene
_L 11 <_>^ i_ y -L^ii uiid-Ld i_ cr v *— *— *— 1
Benzo (b) fluoranthene
Benzo (k) fluoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
11 .
-U-
12 .
n
12.
12.
14 .
14 .
14 .
14 .
14.
16.
16.
17.
. 770
.370—
.347
nnn
.486
.529
O U
. 003
. 046
.468
.559
.703
.653
.653
.214
212
-M-9-
149
228
228
252
252
252
252
252
276
278
276
-7Q-] -7 _-
509002
832944
n
1140788
1079996
1070422
1028663
1009827
985394
944939
918701
770875
746659
.
20
21
18
18
19
18
20
19
17
18
18
18
.41
. 73
N D
.68
.77
. U o
. 18
. 87
.31
.21
.65
.10
.01
.04
^
ug/mL#
ug/mL#
ug/mL
ug/mL
|-iy/ limit
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
100
99
98
99
98
95
99
97
99
97
99
97
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 12:57:05 2011 EA# 0111-20 Page 61 of 126
Page: 2
-------
Calibration
Curve Chromatograms
(Scan)
. '1 v
«'. .!
EA# 0111-20 Page 62 of 126
-------
HI!
#
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
Name
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis(2-Chloroethyl)ether
2-Chlorophenol
1,3-Dichlorobenzene
1,4-Dichlorobenzene (CCC)
Benzyl alcohol
1,2-Dichlorobenzene
2-Methylphenol
bis(2-Chloroisopropyl) ether
3/4-Methylphenol
N-Nitrso-di-n-propylamine (SPCC)
Hexachloroethane
Nitrobenzene
Isophorone
2,4-Dimethylphenol
2-Nitrophenol (CCC)
Benzole acid
bis(2-Chloroethoxy)methane
2,4-Dichlorophenol (CCC)
1 ,2,4-Trichlorobenzene
Naphthalene
4-Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylphenol (CCC)
2-Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadiene (SPCC)
2,4,6-Trichlorophenol (CCC)
2,4,5-Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1,4-Dinitrobenzene
Dimethylphthalate
1,3-Dinitrobenzene
2,6-Dinitrotoluene
1,2-Dinitrobenzene
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2,4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2,4-Dinitrotoluene
Dibenzofuran
2,3,5,6-Tetrachlorophenol
2,3,4,6-Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenylether
Fluorene
4-Nitroaniline
4,6-Dinitro2-methylphenol
N-Nitrosodiphenylamine (CCC)
Azobenzene
4-Bromophenyl-phenylether
Hexachloro benzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3,3-Dimethylbenzidine
bis(2-ethylhexyl)adipate
bis(2-Ethylhexyl)phthalate
3,3'-Dichlorobenzidine
Benzo(a)anthracene
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
0.765
1.22
1.41
0.610
0.802
1.21
1.41
1.43
0.611
1.36
0.918
1.02
1.16
0.793
0.504
1.21
0.581
0.221
0.168
0.0945
0.369
0.214
0.344
1.04
0.106
0.200
0.202
0.636
0.619
0.182
0.333
0.319
1.11
0.241
0.134
1.22
0.168
0.275
0.122
1.67
0.260
1.08
0.0615
0.140
0.312
1.49
0.253
0.268
1.20
0.615
1.18
0.226
0.117
1.01
1.09
0.203
0.212
0.0781
0.964
0.936
0.799
1.00
0.951
0.402
1.06
0.405
0.536
0.377
0.525
0.287
0.987
Response Factor Report
FileName Acq. Date
W1 002873. D 10/18/10 17:56
W1 002874. D 10/18/10 18:26 Inst:
W1 002875. D 10/18/10 18:55
W1 002876. D 10/18/10 19:25 Curve:
W1002877.D 10/18/10 19:54
W1002878.D 10/18/10 20:24
W1002879.D 10/18/10 20:54
illllllll^K^IMIllllllllll
SCAN
gcms22pg1
25
0.750
1.27
1.40
0.609
0.798
1.22
1.41
1.44
0.652
1.36
0.906
0.992
1.16
0.809
0.505
1.19
0.598
0.233
0.177
0.124
0.365
0.247
0.342
1.02
0.109
0.201
0.218
0.630
0.606
0.177
0.356
0.352
1.11
0.263
0.154
1.24
0.187
0.286
0.131
1.69
0.278
1.05
0.0781
0.160
0.342
1.45
0.270
0.279
1.19
0.611
1.17
0.238
0.138
1.02
1.05
0.210
0.214
0.0932
0.953
0.928
0.795
1.07
0.993
0.387
1.08
0.435
0.557
0.397
0.584
0.269
0.969
SCAN
gcms22pg1
25
0.746
1.26
1.36
0.599
0.796
1.19
1.35
1.37
0.658
1.28
0.897
0.981
1.14
0.795
0.493
1.17
0.572
0.273
0.178
0.172
0.353
0.234
0.327
0.978
0.106
0.193
0.230
0.610
0.594
0.197
0.349
0.353
1.05
0.268
0.161
1.16
0.188
0.276
0.126
1.64
0.270
1.00
0.108
0.182
0.342
1.39
0.275
0.286
1.15
0.590
1.13
0.251
0.166
0.970
1.05
0.203
0.205
0.0988
0.898
0.910
0.759
1.01
0.905
0.430
0.980
0.438
0.578
0.397
0.609
0.276
0.947
SCAN
gcms22pg1
25
0.776
1.30
1.31
0.603
0.794
1.20
1.36
1.38
0.643
1.30
0.861
0.982
1.13
0.802
0.512
1.15
0.593
0.305
0.184
0.216
0.355
0.262
0.328
0.957
0.109
0.196
0.256
0.615
0.594
0.218
0.364
0.364
1.05
0.283
0.184
1.20
0.206
0.290
0.137
1.62
0.292
1.02
0.150
0.222
0.368
1.40
0.310
0.312
1.18
0.608
1.16
0.288
0.210
1.01
1.09
0.203
0.211
0.121
0.904
0.915
0.788
1.04
0.947
0.496
1.01
0.451
0.639
0.404
0.645
0.274
0.963
SCAN
gcms22pg1
25
0.804
1.34
1.30
0.601
0.789
1.22
1.39
1.41
0.653
1.32
0.869
0.960
1.14
0.806
0.523
1.13
0.593
0.328
0.192
0.233
0.360
0.271
0.343
0.967
0.111
0.209
0.260
0.620
0.599
0.217
0.380
0.382
1.07
0.295
0.194
1.25
0.219
0.308
0.149
1.62
0.303
1.04
0.170
0.237
0.398
1.42
0.337
0.338
1.22
0.638
1.18
0.305
0.237
1.04
1.08
0.212
0.222
0.134
0.906
0.914
0.812
1.05
0.990
0.557
1.06
0.473
0.677
0.411
0.659
0.284
0.986
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
0.779
1.29
1.24
0.589
0.756
1.17
1.35
1.37
0.644
1.28
0.829
0.921
1.15
0.801
0.514
1.09
0.586
0.335
0.190
0.258
0.350
0.280
0.338
0.912
0.111
0.209
0.273
0.618
0.599
0.233
0.374
0.380
1.03
0.282
0.194
1.17
0.214
0.296
0.141
1.52
0.297
1.01
0.180
0.238
0.375
1.35
0.333
0.333
1.12
0.625
1.12
0.292
0.235
0.982
0.993
0.217
0.229
0.142
0.861
0.875
0.771
0.947
0.914
0.573
0.962
0.444
0.680
0.382
0.610
0.331
0.961
SCAN
gcms22pg1
25
0.771
1.28
1.25
0.581
0.741
1.16
1.32
1.34
0.653
1.26
0.856
0.913
1.16
0.790
0.506
1.10
0.565
0.322
0.190
0.258
0.337
0.277
0.331
0.868
0.107
0.207
0.270
0.590
0.566
0.222
0.378
0.391
1.00
0.285
0.201
1.19
0.221
0.305
0.147
1.49
0.304
1.00
0.195
0.248
0.391
1.33
0.354
0.341
1.12
0.637
1.13
0.307
0.253
1.01
0.982
0.218
0.228
0.144
0.834
0.833
0.757
0.883
0.877
0.549
0.911
0.448
0.686
0.392
0.611
0.348
0.947
Average RF
0.770
1.28
1.32
0.599
0.782
1.19
1.37
1.39
0.645
1.31
0.876
0.966
1.15
0.799
0.508
1.15
0.584
0.288
0.183
0.194
0.356
0.255
0.336
0.963
0.108
0.202
0.244
0.617
0.597
0.206
0.362
0.363
1.06
0.274
0.175
1.20
0.201
0.291
0.136
1.61
0.286
1.03
0.135
0.204
0.361
1.40
0.305
0.308
1.17
0.618
1.15
0.272
0.194
1.01
1.05
0.210
0.217
0.116
0.903
0.902
0.783
1.00
0.940
0.485
1.01
0.442
0.622
0.394
0.606
0.295
0.966
%RSD
2.53%
2.86%
4.92%
1.76%
3.03%
2.03%
2.39%
2.74%
2.46%
3.08%
3.59%
3.88%
1.08%
0.88%
1.83%
3.86%
2.07%
16.2%
4.80%
33.6%
3.01%
9.58%
2.16%
6.20%
1.99%
3.13%
11.3%
2.43%
2.72%
10.3%
4.76%
6.72%
3.77%
6.61%
14.4%
2.61%
9.86%
4.50%
7.46%
4.55%
6.00%
2.83%
38.9%
21.0%
8.45%
3.93%
12.7%
9.89%
3.42%
2.75%
2.19%
12.2%
27.6%
2.42%
4.20%
3.21%
4.23%
22.4%
5.10%
3.98%
2.72%
6.64%
4.61%
16.1%
6.18%
4.65%
10.3%
3.06%
7.17%
10.5%
1.70%
Pass/Fail
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Warning
Pass
Pass
Pass
Pass
Pass
Pass
Pass
EA# 0111-20 Page 63 of 126
-------
Sample ID LID
SSTD005-SCANgcms22pg125 5
SSTD010-SCANgcms22pg125 10
SSTD020-SCANgcms22pg125 20
SSTD050-SCANgcms22pg125 50
SSTD080-SCANgcms22pg125 80
SSTD120-SCAN gcms22pg125 120
SSTD160-SCAN gcms22pg125 160
Response Factor Report
FileName Acq. Date
W1002873. D 10/18/10 17:56
W1002874. D 10/18/10 18:26
W1002875. D 10/18/10 18:55
W1002876. D 10/18/10 19:25
W1002877. D 10/18/10 19:54
W1002878.D 10/18/10 20:24
W1002879.D 10/18/10 20:54
Inst:
Wiley
Curve: W101810X-8270.M
SCAN
gcms22pg1
# Name 25
85) Chrysene 0.936
86) Di-n-octylphthalate (CCC) 0.760
88) Benzo(b)fluoranthene 0.943
89) Benzo(k)fluoranthene 0.945
90) Benzo(e)pyrene 0.864
91) Benzo(a)pyrene (CCC) 0.859
92) Perylene 0.956
93) lndeno(1,2,3-cd)pyrene 0.879
94) Dibenz(a,h)anthracene 0.723
95) Benzo(g,h,i)perylene 0.744
SPCC Average RF must be > 0.05
CCC %RSD must be <= 30%
Other %RSD<= 15%
Average %RSD <= 15%
No %RSD > 50%
SCAN
gcms22pg1
25
0.933
0.967
0.983
0.881
0.882
0.966
0.842
0.701
0.707
SCAN
gcms22pg1
25
0.887
0.977
0.920
0.952
0.857
0.882
0.933
0.966
0.808
0.815
SCAN
gcms22pg1
25
0.913
1.06
0.973
0.969
0.874
0.916
0.942
0.984
0.828
0.812
SCAN SCAN
gcms22pg1 gcms22pg1
25
0.892
25
0.922
1.06
1.09
1.01
0.938
0.985
1.00
0.937
0.793
0.747
1.00
1.01
1.00
0.906
0.951
0.962
0.909
0.781
0.711
SCAN
gcms22pg1
25
0.885
0.963
1.10
0.979
0.918
0.962
0.963
0.851
0.737
0.657
Average RF
0.910
0.957
1.00
0.977
0.891
0.920
0.960
0.910
0.767
0.742
%RSD
2.40%
11.0%
7.04%
2.46%
3.39%
5.17%
2.07%
6.09%
6.15%
7.72%
Pass/Fail
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Pass
Average % 6.7%
EA# 0111-20 Page 64 of 126
-------
Relative Retention Time Report
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
# Name
2) N-Nitrosodimethylamine
3) Pyridine
6) Phenol (CCC)
7) Aniline
8) bis(2-Chloroethyl)ether
9) 2-Chlorophenol
10) 1,3-Dichlorobenzene
11) 1,4-Dichlorobenzene (CCC)
12) Benzyl alcohol
13) 1,2-Dichlorobenzene
14) 2-Methylphenol
15) bis(2-Chloroisopropyl) ether
16) 3/4-Methylphenol
17) N-Nitrso-di-n-propylamine (SPCC)
18) Hexachloroethane
20) Nitrobenzene
22) Isophorone
23) 2,4-Dimethylphenol
24) 2-Nitrophenol (CCC)
25) Benzole acid
26) bis(2-Chloroethoxy)methane
27) 2,4-Dichlorophenol (CCC)
28) 1,2,4-Trichlorobenzene
29) Naphthalene
30) 4-Chloroaniline
31) Hexachlorobutadiene (CCC)
32) 4-Chloro-3 methylphenol (CCC)
33) 2-Methylnaphthalene
34) 1-Methylnaphthalene
35) Hexachlorocyclopentadiene (SPCC)
37) 2,4,6-Trichlorophenol (CCC)
38) 2,4,5-Trichlorophenol
40) 2-Chloronaphthalene
41) 2-Nitroaniline
42) 1,4-Dinitrobenzene
43) Dimethylphthalate
44) 1,3-Dinitrobenzene
45) 2,6-Dinitrotoluene
46) 1,2-Dinitrobenzene
47) Acenaphthylene
48) 3-Nitroaniline
49) Acenaphthene (CCC)
50) 2,4-Dinitrophenol (SPCC)
51) 4-Nitrophenol (SPCC)
52) 2,4-Dinitrotoluene
53) Dibenzofuran
54) 2,3,5,6-Tetrachlorophenol
55) 2,3,4,6-Tetrachlorophenol
56) Diethlyphthalate
58) 4-Chlorophenyl-phenylether
59) Fluorene
60) 4-Nitroaniline
61) 4,6-Dinitro2-methylphenol
62) N-Nitrosodiphenylamine (CCC)
63) Azobenzene
66) 4-Bromophenyl-phenylether
67) Hexachlorobenzene
68) Pentachlorophenol (CCC)
69) Phenanthrene
70) Anthracene
71) Carbazole
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.97
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Acq. Date
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.01
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.94
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
17:56
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
0.60
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.97
0.99
1.00
1.00
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.95
0.96
0.96
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Inst:
Curve:
SCAN
gcms22pg1
25
0.60
0.61
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.97
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.07
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
0.61
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.01
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.07
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
SCAN
gcms22pg1
25
0.61
0.62
0.93
0.94
0.95
0.97
0.99
1.00
1.02
1.03
1.03
1.04
1.06
1.06
1.09
1.10
0.93
0.94
0.94
0.96
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.01
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.07
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
Average
RRT
0.60
0.62
0.93
0.94
0.95
0.96
0.99
1.00
1.02
1.03
1.03
1.04
1.05
1.06
1.09
1.10
0.93
0.94
0.94
0.95
0.95
0.98
0.99
1.00
1.01
1.02
1.07
1.11
1.12
1.13
0.90
0.91
0.93
0.94
0.96
0.96
0.97
0.97
0.98
0.98
0.99
1.00
1.00
1.00
1.02
1.02
1.03
1.04
1.04
1.06
1.07
1.06
1.07
1.08
1.08
0.95
0.96
0.98
1.00
1.01
1.02
EA# 0111-20 Page 65 of 126
-------
Relative Retention Time Report
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
# Name
72) Di-n-butylphthalate
73) Fluoranthene (CCC)
74) Benzidine
76) Pyrene
79) Butylbenzylphthalate
80) 3,3-Dimethylbenzidine
81) bis(2-ethylhexyl)adipate
82) bis(2-Ethylhexyl)phthalate
83) 3,3'-Dichlorobenzidine
84) Benzo(a)anthracene
85) Chrysene
86) Di-n-octylphthalate (CCC)
88) Benzo(b)fluoranthene
89) Benzo(k)fluoranthene
90) Benzo(e)pyrene
91) Benzo(a)pyrene (CCC)
92) Perylene
93) lndeno(1,2,3-cd)pyrene
94) Dibenz(a,h)anthracene
95) Benzo(g,h,i)perylene
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Acq. Date
10/18/10 17:56
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.95
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Inst:
Curve:
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
SCAN
gcms22pg1
25
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.05
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
Average
RRT
1.05
1.13
1.13
1.15
0.94
0.94
0.94
0.99
0.99
1.00
1.00
1.06
0.96
0.96
0.99
0.99
1.00
1.14
1.14
1.18
= RRT outside 0.06 unit RRT window
EA# 0111-20 Page 66 of 126
-------
Internal Standards Retention Time Report
#
1)
21)
36)
64)
78)
87)
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-d8 (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
= ISTD retention time outside of 20 sec (0.33 min) window
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.89
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.89
Acq. Date
10/18/10 17:56
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
5.38
6.58
8.34
9.85
12.66
14.90
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.85
12.66
14.90
Inst:
Curve:
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.85
12.67
14.90
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.86
12.67
14.90
SCAN
gcms22pg1
25
5.38
6.59
8.35
9.86
12.67
14.90
Average
5.38
6.59
8.34
9.85
12.67
14.90
EA# 0111-20 Page 67 of 126
-------
Internal Standards Area Report
#
1)
21)
36)
64)
78)
87)
Sample ID
SSTD005-SCAN gcms22pg125
SSTD010-SCAN gcms22pg125
SSTD020-SCAN gcms22pg125
SSTD050-SCAN gcms22pg125
SSTD080-SCAN gcms22pg125
SSTD120-SCAN gcms22pg125
SSTD160-SCAN gcms22pg125
Name
1,4-Dichlorobenzene-d4 (I)
Naphthalene-dS (I)
Acenaphthene-d10 (I)
Phenanthrene-d10 (I)
Chrysene-d12 (I)
Perylene-d12 (I)
= ISTD response outside of 40% window
LID
5
10
20
50
80
120
160
SCAN
gcms22pg1
25
694,978
2,450,968
1,342,042
2,316,172
2,072,984
1,889,069
FileName
W1002873.D
W1002874.D
W1002875.D
W1002876.D
W1002877.D
W1002878.D
W1002879.D
SCAN
gcms22pg1
25
706,693
2,486,145
1,339,110
2,294,140
2,146,192
1,945,018
Acq. Date
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
10/18/10
SCAN
gcms22pg1
25
720,068
2,542,711
1,390,154
2,354,525
2,151,499
2,135,523
17:56
18:26
18:55
19:25
19:54
20:24
20:54
SCAN
gcms22pg1
25
655,591
2,346,284
1,314,587
2,336,676
2,279,787
2,168,681
Inst:
Curve:
SCAN
gcms22pg1
25
662,846
2,259,727
1,246,926
2,303,538
2,250,870
1,934,135
Wiley
W101810X-8270.M
SCAN
gcms22pg1
25
708,059
2,432,537
1,402,728
2,472,354
2,393,551
2,217,223
SCAN
gcms22pg1
25
673,532
2,366,580
1 ,358,679
2,516,253
2,291,395
1 ,886,569
Average
688,824
2,412,136
1,342,032
2,370,523
2,226,611
2,025,174
EA# 0111-20 Page 68 of 126
-------
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-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 10:41:33 2010
Initial Calibration
Quant Title
QLast Update
Response via
TIC: W1002873.D\data.ms
Abundance
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000 f.
500000
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Fri Oct 22 14:30:55 2010EA# 0111-20 Page 72 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:41:33 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
583
340
852
662
890
152
136
164
188
240
264
694978
2450968
1342042
2316172
2072984
1889069
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
243
964
872
624
858
136
311
423
112
99
82
172
176
330
212
244
90746
113905
110324
218740
187221
22208
242828
190827
4
5
5
5
5
4
5
4
79
17
10
27
08
19
03
97
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
At }
1-L 1
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
r^ TI T • j *-*-.— * T-I ' n ' T-I f~.
z, - IN i L. roani j. ine
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
•7
/
7
7
8
8
8
249
313
975
065
082
183
333
391
455
536
530
568
664
691
861
888
097
177
193
193
267
417
519
604
620
711
058
283
384
437
544
582
774
P A Q
o *± y
961
993
041
068
137
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
C [T
O D
168
163
168
165
168
66491
106190
122087
53017
69681
104979
122495
124574
53085
118211
79720
88243
100657
68876
43746
105233
177985
67707m
51599
28941
112965
65593
105411
318534
32494
61216
61911
194818
189695
55760
55851
53497
186345
A r\ ~i c p
*± U Jj D o
22517m
204773
28240
46163
20495
4
4
5
5
5
5
5
5
4
5
5
5
5
4
4
5
4
3
4
2
5
4
5
5
4
4
4
5
5
4
4
4
5
3
5
4
4
4
97
77
31
10
13
06
14
16
74
21
24
26
05
96
95
27
97
72
61
44
18
16
12
40
89
94
14
15
19
41
60
39
24
A r\
*± U
85
07
20
73
48
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
-0 . 02
-0 . 01
0 . 00
-0 . 01
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
/ T \\
•jig/mLiff
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
91
83
91
91
88
96
99
96
85
95
98
81
94
67
87
85
Manual Int. "II" (Et)E^|
92
1
96
95
98
93
77
98
89
100
97
100
98
90
95
[Manual Int. "WP" (EbE^
99
62
84
89
W101810X-8270 .M Fri Oct 22 14:30:55 2010 EA# 0111-20 Page 73 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002873.D
18 Oct 2010 5:56 pm
dda
SSTD005-SCAN gcms22pg!25
gcmsprep2pg3 0
6 Sample Multiplier: 1
Quant Time Oct 20 12:12:33 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:41:33 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
201
255
372
356
372
485
543
613
655
693
858
891
869
901
965
019
355
462
644
873
927
066
333
086
166
327
871
904
898
497
555
646
694
362
217
259
698
788
933
947
947
540
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
280459
43562
181422
10321
23474
52380
249463
42498
44942
201374
103250
197862
37898
19583
168974
182189
58744
61475
22599
279098
270861
231460
290388
275456
116352
306508
104995
138785
97568
136132
74279
255683
242579
196874
222593
223219
204034
202852
225687
207540m
170747
175572
5
4
5
2
3
4
5
4
4
5
4
5
4
3
5
5
4
4
3
5
5
5
5
5
4
5
4
4
4
4
4
5
5
3
4
4
4
4
4
5
4
5
20
54
26
28
45
32
30
16
32
14
98
12
15
03
01
19
84
88
37
34
19
10
01
06
15
25
58
31
77
33
85
10
15
97
71
85
85
66
99
92
71
01
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
98
74
94
100
81
92
95
97
75
100
95
100
81
17
95
100
98
94
92
98
99
98
97
98
92
99
100
100
100
100
95
98
99
100
99
94
99
98
QQ
Manual Int. "WP" (Et)E)|
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:30:55 2010 EA# 0111-20 Page 74 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:24:37 2010
Initial Calibration
Quant Title
QLast Update
Response via
TIC: W1002874.D\data.ms
Abundance
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00
W101810X-8270 .M Fri Oct 22 14:43:19 2010EA# 0111-20 Page 75 of 126
13.00 14.00 15.00 16.00
17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:24:37 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
583
340
852
662
890
152
136
164
188
240
264
706693
2486145
1339110
2294140
2146192
1945018
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
238
964
872
625
859
136
311
423
112
99
82
172
176
330
212
244
189663
231671
219110
444619
366528
48270
497272
393732
9
10
9
10
9
9
10
10
84
34
96
74
97
19
40
34
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
^ — ' /
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
I sophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalat e
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
7
8
8
8
244
308
975
066
082
183
333
392
456
541
530
568
664
691
862
888
102
177
193
204
268
423
519
604
620
711
058
283
384
438
544
582
774
849
961
993
047
068
137
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
132455
223500
246658
107534
141015
215831
248947
254240
115259
239817
160017
175237
204756
142924
89308
209432
371720
144581m
109870
77311
227084
153620
212317
635629
67758
124967
135411
391569
376496
109877
119287
117762
370284
87885
51444m
413512
62670
95709
43995
9
9
10
10
10
10
10
10
10
10
10
10
10
10
9
10
10
7
9
6
10
9
10
10
10
9
8
10
10
8
9
9
10
9
8
10
9
9
9
73
87
54
16
20
22
28
35
12
38
34
26
10
12
95
32
24
95
67
42
27
69
16
62
05
95
93
21
15
56
84
69
43
59
57
26
33
82
64
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
ug/mL 0.00
Qvalue
ug/mL 91
ug/mL# 86
ug/mL# 91
ug/mL 91
ug/mL 87
ug/mL 96
ug/mL 98
ug/mL 98
ug/mL 84
ug/mL 96
ug/mL 95
ug/mL# 83
ug/mL 96
ug/mL# 71
ug/mL 87
ug/mL 86
•|irj/mT. CW
ug/mL [Manual Int. "II" (EOE)|
ug/mL 94
ug/mL# 1
ug/mL 97
ug/mL 97
ug/mL 98
ug/mL 91
ug/mL 75
ug/mL 99
ug/mL 91
ug/mL 99
ug/mL 100
ug/mL# 100
ug/mL 99
ug/mL# 92
ug/mL 96
ug/mL 84
j^S- Manual Int. "WP" (EDE^
ug/mL# 74
ug/mL# 84
ug/mL 89
W101810X-8270 .M Fri Oct 22 14:43:18 2010 EA# 0111-20 Page 76 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002874.D
18 Oct 2010 6:26 pm
dda
SSTD010-SCAN gcms22pg!25
gcmsprep2pg3 0
7 Sample Multiplier: 1
Quant Time Oct 20 12:13:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:24:37 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
Q A \
y4 ;
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
-i /-
16
17
202
255
373
356
373
485
543
613
656
693
859
891
869
901
965
019
355
462
644
879
927
066
333
086
172
327
872
904
898
491
555
646
694
362
217
260
698
789
938
947
QC1
y bz
540
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
O ""7 O
2 la
276
565311
93155
351625
26144
53475
114585
485032
90233
93540
399969
204672
391149
79786
46162
342496
350420
120530
123010
53464
546524
532469
455826
614998
569316
221945
621484
233209
298947
213098
313443
144361
519832
500412
476626
470026m
478117
428593
429107
469953
409345m
Q A "i r\ ""? Q
3410 /3
343597
10
9
10
5
7
9
10
8
9
10
9
10
8
7
10
10
10
9
8
10
10
10
10
10
7
10
9
8
10
9
9
10
10
9
9
10
9
9
10
11
9
50
72
21
80
87
47
33
85
06
23
90
14
75
12
18
00
03
87
05
55
30
15
70
56
98
74
83
96
07
64
11
03
25
28
66
06
89
60
07
35
"1 A
14
52
ug/mL 98
ug/mL# 76
ug/mL 96
ug/mL# 100
ug/mL# 69
ug/mL 90
ug/mL 96
ug/mL 98
ug/mL# 78
ug/mL 99
ug/mL 96
ug/mL 99
ug/mL# 83
ug/mL# 12
ug/mL 95
ug/mL# 100
ug/mL 97
ug/mL 95
ug/mL 91
ug/mL 100
ug/mL 100
ug/mL 99
ug/mL 97
ug/mL 98
ug/mL 100
ug/mL# 99
ug/mL# 100
ug/mL# 100
ug/mL# 100
ug/mL# 99
ug/mL 100
ug/mL 98
ug/mL 99
ug/mL# 100
ug/mL
ug/mL 96
ug/mL 99
ug/mL 98
ug/mL 98
ug/mL
-1 1 /T / TVt T 44- Q /"""
ug/mL# 96
ug/mL 99
Manual Int. "WP" (Et)E^|
Manual Int. "WP" (Et)E)|
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:43:18 2010 EA# 0111-20 Page 77 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:28:56 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
TIC: W1002875.D\data.ms
§ I
E2
qo
~
. -=103 O
' BO
- ~rP,
>.
o
o
o
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Fri Oct 22 14:43:34 2010EA# 0111-20 Page 78 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:28:56 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 583
.340
. 852
. 662
.895
152
136
164
188
240
264
720068
2542711
1390154
2354525
2151499
2135523
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
. 964
. 872
. 624
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
385102
471390
454648
861131
734622
98664
938517
721401
19
20
20
20
19
18
19
18
.62
. 65
.28
. 03
.24
.30
.12
.47
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7 .
7 .
7 .
8.
8.
8.
.249
.308
. 980
.066
.082
.183
.333
.391
.456
. 541
. 530
.568
.664
.691
.862
. 888
. 102
. 177
.193
.219
.268
.422
.519
. 609
. 620
. 711
. 058
.283
.384
.438
.544
. 582
. 774
. 849
. 966
. 998
.047
.073
.137
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
268662
454960
488820
215533
286491
427306
487385
491902
236968
461266
322944
353103
412029
286292
177593
420879
727219
346769m
226482
218584
448403
298031
415380
1242897
135275
245812
292277
775011
755442
250781
242401
245683
732264
186464
111999m
808845
130890
191913
87855
19
19
20
19
20
19
19
19
20
19
20
20
19
19
19
20
19
18
19
17
19
18
19
20
19
19
18
19
19
19
19
19
19
19
17
19
18
18
18
.38
. 73
. 51
.99
.34
.87
.75
. 65
.41
. 60
.47
.30
.95
.90
.41
.36
.59
.90
.49
.74
.84
.38
.44
.30
. 62
. 13
. 84
.76
.92
.11
.27
.48
. 86
. 61
. 50
. 32
.77
.98
.55
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
93
82
92
91
89
95
99
99
84
94
96
82
96
71
87
86
en
Manual Int. "II" (EOE)|
92
1
97
95
98
89
77
99
90
99
99
100
98
94
94
84
jManual Int. "WP" (Et)E^|
76
87
89
W101810X-8270 .M Fri Oct 22 14:43:33 2010 EA# 0111-20 Page 79 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002875.D
18 Oct 2010 6:55 pm
dda
SSTD020-SCAN gcms22pg!25
gcmsprep2pg3 0
8 Sample Multiplier: 1
Quant Time Oct 20 12:13:56 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:28:56 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
201
255
372
356
372
485
543
607
656
693
859
891
875
901
971
019
355
468
644
879
927
066
333
086
172
327
872
904
898
497
555
646
694
362
217
260
703
794
938
952
952
545
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
1138687
187783
696007
75132
126824
237923
966279
191404
198571
796177
410164
788273
174390
115410
674512
731513
238946
241102
116269
1057464
1071540
893014
1194294
1065299
506627
1153486
471590
621800
427604
655163
296700
1018235
954008
1050503
981816
1016689
914623
942058
996589
1031781m
863105
870239
20
18
19
16
17
18
19
18
18
19
19
19
18
17
19
20
19
18
17
19
20
19
20
19
17
19
19
18
20
20
18
19
19
20
18
19
19
19
19
26
21
21
37
87
46
05
99
95
82
08
54
61
10
69
43
15
31
10
37
84
07
89
19
37
25
26
75
41
83
59
16
10
67
61
50
40
39
49
22
19
45
05
07
97
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
98
72
95
100
70
90
94
97
81
99
96
99
81
11
95
100
100
95
90
100
99
99
98
97
99
99
100
100
100
99
99
98
99
100
97
95
99
97
|Manual Int. "WP" (EDE^
96
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:43:33 2010 EA# 0111-20 Page 80 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Abundance
1.2e+07
1.15e+07
1.16+07
1.056+07
1e+07
9500000
9000000
8500000
8000000
7500000
7000000
6500000
6000000
5500000
5000000
4500000
4000000
3500000
3000000
2500000
2000000
1500000
1000000
500000
n
TIC: W1002876.D\data.ms
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W101810X-8270 .M Fri Oct 22 14:43:50 2010EA# 0111-20 Page 81 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 588
.346
. 852
. 662
.895
152
136
164
188
240
264
655591
2346284
1314587
2336676
2279787
2168681
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
.969
. 872
. 625
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
900585
1027693
1032667
2022693
1817663
262774
2440734
1933841
50
49
50
49
50
49
50
49
.39
.44
. 60
. 76
.35
.12
.11
.88
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41 )
*± _L j
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2 -Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7
7 .
7.
8.
8.
8.
.249
.308
. 980
.066
.087
.188
.333
.391
.461
. 541
. 536
.573
.669
.696
.862
. 894
. 107
. 177
. 198
.241
.273
.423
.519
. 610
. 620
. 711
. 058
.283
.384
.438
.544
. 582
. 779
84 Q
. O ^t ^1
. 966
.998
.052
.073
.143
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
635596
1062640
1074210
494387
650353
979940
1116124
1134699
526916
1065553
705677
804523
924560
656842
419458
944597
1738085
895507
539145
634258
1042176
767304
963119
2807524
320545
574376
750985
1804232
1742830
638121
597498
598687
1729488
465188
301749m
1964791
339018
477324
225093
50
50
49
50
50
50
49
49
49
49
49
50
49
50
50
50
50
52
50
55
49
51
48
49
50
48
52
49
49
52
50
50
49
51
48
49
51
49
50
.35
. 61
. 50
.37
.72
.04
.68
.79
. 86
. 74
. 14
.80
.16
.14
.36
. 18
. 74
.91
.28
.80
.96
.28
.84
. 68
.38
.44
.45
.86
.79
.70
.23
.19
. 61
. 72
. 86
.64
.42
.91
.25
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
UQ/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
91
82
91
89
89
96
98
99
85
95
97
82
94
72
87
87
96
61
93
1
97
96
98
86
75
99
91
99
99
100
99
90
94
0 C
iManual Int. "WP" (Et)E^|
99
71
72
89
W101810X-8270 .M Fri Oct 22 14:43:49 2010 EA# 0111-20 Page 82 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002876.D
18 Oct 2010 7:25 pm
dda
SSTD050-SCAN gcms22pg!25
gcmsprep2pg3 0
9 Sample Multiplier: 1
Quant Time Oct 20 10:47:01 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:30:55 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8.
8.
8 .
8 .
8 .
8 .
8.
8.
8.
8.
8 .
8 .
8 .
8 .
8.
9.
9.
9.
9.
9.
9.
10 .
10.
11.
11.
11.
11 .
11 .
11 .
12 .
12.
12.
12.
13.
14 .
14 .
14 .
14 .
14 .
16.
16 .
17.
.207
.260
.378
.356
.378
.490
.543
.613
.656
.698
. 859
. 896
. 880
. 907
.971
.019
.361
.468
. 644
. 879
. 932
. 066
.338
.092
.172
.332
. 877
. 904
. 898
.497
.561
.646
.700
.362
.222
.270
. 703
.799
. 943
.963
. 968
.556
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
2670061
479906
1669970
247053
363991
605293
2297357
508913
513321
1941442
999101
1910816
472667
345178
1651904
1784554
593204
615677
352570
2640876
2674012
2302237
3044317
2764843
1448854
2949812
1286236
1821139
1151877
1837456
779586
2743401
2601527
3014003
2638192
2627687
2368320
2483685
2553222
2666636m
2243251
2201757
50
51
49
55
54
50
49
50
50
50
49
50
52
54
50
51
48
48
52
50
50
50
52
50
51
50
51
51
51
53
46
49
50
55
48
49
49
49
49
66
53
54
.50
.01
.38
. 80
.59
.97
.83
.84
.67
.56
.21
.46
. 83
.25
.00
.85
.45
.49
. 15
. 06
. 77
.33
.01
.37
.15
.03
. 04
.38
.25
.19
.30
.85
.18
.25
. 65
. 60
. 02
. 83
. 07
.30
. 92
.74
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
97
72
94
100
70
90
94
97
81
99
96
99
85
12
96
100
99
94
90
99
100
99
99
98
99
98
100
100
100
100
99
99
99
100
98
95
99
97
i nn
iManual Int. "WP" (Et)E^|
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:43:49 2010 EA# 0111-20 Page 83 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:32:38 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.7e+07
1.6e+07
1.56+07
1.46+07
1.3e+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
a
JE
£
eg,
TIC: W1002877.D\data.ms
«
-20
-=
§00
LL -O
I I
O
O
O
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Fri Oct 22 14:44:07 2010EA# 0111-20 Page 84 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:32:38 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
852
667
895
152
136
164
188
240
264
662846
2259727
1246926
2303538
2250870
1934135
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
243
975
877
624
864
136
316
423
112
99
82
172
176
330
212
244
1520406
1676087
1643568
3079250
2847479
450730
4032039
3278436
84
79
79
79
83
85
83
85
13
75
65
87
16
46
98
79
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
8
249
302
985
071
087
188
333
391
461
541
536
573
674
701
861
893
107
182
198
257
273
422
518
609
625
711
063
282
389
437
544
582
779
854
972
004
057
078
148
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
1065824
1777926
1729656
797205
1046624
1618701
1841092
1864578
865190
1745698
1151734
1272195
1506894
1068651
693404
1499787
2678725
1483117
868327
1054266
1628238
1226169
1550822
4368870
501374
942468
1175601
2802443
2709029
979053
948267
952771
2680042
734512
484493m
3112785
546881
769244
370447
83
83
78
80
80
81
81
80
80
80
79
79
79
80
82
78
81
90
84
96
81
85
81
80
81
82
85
80
80
83
84
84
81
86
81
82
87
84
87
51
75
83
33
73
75
06
93
97
59
32
45
24
68
34
80
20
99
08
30
05
09
66
28
81
53
26
41
37
96
04
20
04
10
29
91
44
80
19
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
90
83
92
88
91
95
99
99
86
95
97
82
95
71
89
86
96
79
91
1
97
96
99
86
75
99
93
100
99
100
99
89
94
Rfi
jManual Int. "WP" (EbE^
9 y
68
74
88
W101810X-8270 .M Fri Oct 22 14:44:06 2010 EA# 0111-20 Page 85 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002877.D
18 Oct 2010 7:54 pm
dda
SSTD080-SCAN gcms22pg!25
gcmsprep2pg3 0
10 Sample Multiplier: 1
Quant Time Oct 20 10:45:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:32:38 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8.
8.
8 .
8 .
8 .
8 .
8.
8.
8.
8.
8 .
8 .
8 .
8 .
8.
9.
9.
9.
9.
9.
9.
10 .
10.
11.
11.
11.
11 .
11 .
11 .
12 .
12.
12.
12.
13.
14 .
14 .
14 .
14 .
14.
16.
16.
17.
.207
.260
.378
.362
.383
.490
.549
.613
.655
.698
. 858
. 896
. 885
. 912
.971
.024
.361
.467
. 644
. 879
. 932
. 071
.338
.091
.172
.337
. 877
.909
. 898
.496
.561
.651
.699
.362
.227
.270
. 708
. 804
.949
.968
.973
.561
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
4042909
756365
2583448
422751
590584
991694
3539646
841349
843608
3035443
1590412
2932322
759570
591276
2602966
2703666
978655
1022518
615106
4174914
4211925
3742990
4846729
4561098
2566248
4884640
2130889
3049279
1851064
2966387
1278774
4440131
4151696
4770907
4214544
3922979m
3629102
3812109
3851179
3625684m
3067089
2891515
80
84
80
100
92
88
80
88
87
83
82
81
89
97
83
82
81
81
92
80
81
83
84
84
91
84
85
87
83
86
76
81
81
88
87
83
84
85
82
101
82
80
.61
.76
. 54
. 67
.94
. 05
.95
.61
.79
.34
. 58
. 64
. 50
.97
.06
.82
.09
.69
.29
.28
. 12
. 00
.00
.29
.90
.04
. 64
. 14
.42
.97
.92
.72
.11
.58
. 14
. 03
.22
. 76
.99
.07
.66
.60
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
96
74
94
100
72
88
93
98
82
98
97
100
85
10
96
100
98
92
89
99
99
99
99
97
99
97
100
100
100
99
98
98
100
100
llManual Int. "WP" (EbE^I
100
97
100
^jManual Int. "WP" (EDE)|
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:44:06 2010 EA# 0111-20 Page 86 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
8270C - Full List
Tue Oct 19 11:34:25 2010
Initial Calibration
Quant Title
QLast Update
Response via
TIC: W1002878.D\data.ms
3000000
2000000
1000000
0
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Fri Oct 22 14:44:24 2010EA# 0111-20 Page 87 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:34:25 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
857
673
901
152
136
164
188
240
264
708059
2432537
1402728
2472354
2393551
2217223
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
#0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
248
975
878
624
869
142
321
423
112
99
82
172
176
330
212
244
2357516
2560855
2572034
4954156
4539684
763894
6105122
4961073
122
114
116
114
117
134
118
120
13
06
69
22
86
95
47
95
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2 -Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
8
254
308
991
071
092
188
333
391
466
541
536
573
680
707
862
899
118
193
198
278
278
428
519
609
625
711
064
288
389
443
550
587
779
854
977
009
063
084
153
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
1655479
2748558
2638757
1250650
1606548
2488942
2860266
2903770
1367289
2714894
1761446
1956537
2433885
1701666
1091581
2314661
4279898
2442858
1390088
1884010
2556990
2045970
2465876
6652351
810900
1526411
1993072
4510862
4367649
1697507
1575736
1597090
4326768
1185450
816936m
4936719
900830
1243711
593585
121
121
112
117
116
117
117
117
119
117
113
114
119
120
121
113
120
139
125
159
118
131
120
113
122
124
134
120
120
135
124
125
116
123
119
116
128
121
124
42
20
58
97
01
68
89
98
78
33
57
38
82
26
34
84
52
42
04
86
23
90
61
55
92
17
27
24
36
23
13
47
31
53
66
89
04
87
19
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ucr/mL
|_L^-J / LLLJ-J
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
85
90
95
99
99
86
95
97
81
95
71
91
89
95
95
92
1
97
96
98
82
73
99
91
100
99
100
100
89
93
H-7
Manual Int. "WP" (EbE^
72
76
88
W101810X-8270 .M Fri Oct 22 14:44:24 2010 EA# 0111-20 Page 88 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002878.D
18 Oct 2010 8:24 pm
dda
SSTD120-SCAN gcms22pg!25
gcmsprep2pg3 0
11 Sample Multiplier: 1
Quant Time Oct 20 12:14:38 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:34:25 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
212
271
383
367
388
495
549
618
661
704
864
901
891
917
976
024
361
473
649
884
938
071
338
097
177
343
877
909
898
497
566
657
710
367
238
281
719
815
959
989
984
577
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
6412169
1247776
4267963
758110
1003648
1578371
5664751
1400720
1402198
4716072
2628420
4706583
1227582
987659
4133249
4180139
1612330
1701383
1052185
6387152
6488275
5721649
7022165
6782097
4250745
7136986
3186427
4879382
2743784
4377746
2373255
6897657
6402828
7148627
6718692
6627053
6027853
6326927
6400912
6045974m
5197283
4731566
113
124
118
160
140
124
115
131
129
115
121
116
128
145
117
113
124
126
147
114
116
118
113
116
141
114
120
131
116
120
134
119
117
124
121
122
122
124
120
147
122
115
65
30
28
48
40
57
15
14
71
11
32
49
57
47
24
83
47
64
10
44
42
21
39
78
82
40
43
13
28
70
24
38
64
81
18
38
03
14
33
02
19
06
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
95
76
92
100
74
87
92
98
82
97
98
99
88
10
97
100
97
90
89
97
98
99
99
96
98
96
100
100
100
98
99
97
99
100
98
94
100
96
3Q
[Manual Int. "WP" (EbE^
/
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:44:24 2010 EA# 0111-20 Page 89 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
TIC: W1002879.D\data.ms
Abundance
3.4e+07
3.2e+07
3e+07
2.8e+07
2.6e+07
2.4e+07
2.26+07
2e+07
1.86+07
1.66+07
1.46+07
1.26+07
1e+07
8000000
6000000
4000000
2000000
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Fri Oct 22 14:44:53 2010EA# 0111-20 Page 90 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
375
588
346
858
673
901
152
136
164
188
240
264
673532
2366580
1358679
2516253
2291395
1886569
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
# 0.
0 .
0 .
0 .
0 .
0.
00
00
00
00
00
00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
248
980
883
630
869
142
321
428
112
99
82
172
176
330
212
244
2937218
3293639
3320235
6215938
5946373
1063166
8046097
6485723
159
154
158
147
159
184
153
155
96
22
36
96
38
54
41
36
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
A "1 \
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
5
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
8
8
8
8
255
308
991
076
092
194
333
391
472
541
541
573
680
712
867
899
118
198
203
294
278
428
524
609
625
711
064
288
389
443
550
587
779
860
977
014
068
089
159
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
2078189
3461744
3371914
1566019
1996432
3120918
3568480
3597095
1759118
3382190
2305638
2460461
3125251
2127745
1364287
2965421
5347160
3051656
1796992
2443160
3186218
2620544
3137769
8215827
1008461
1961876
2554086
5580915
5353707
2101657
2051735
2123898
5435141
1548645
1091043m
64896 98
1200737
1659543
801577
160
160
151
155
151
155
154
153
162
153
156
151
161
158
159
153
154
179
166
213
151
173
157
144
157
164
176
152
151
172
166
172
150
166
162
158
176
167
173
24
48
24
29
55
12
62
65
01
67
25
22
74
08
43
33
77
01
15
08
43
64
75
15
13
04
83
90
65
10
87
27
84
60
60
64
20
89
15
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
0.00
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
82
91
95
99
99
86
94
9
6
80
95
71
93
88
94
97
93
1
97
96
98
81
75
99
93
99
100
100
99
90
92
87
9
(Manual Int. "WP" (EbE^
o
75
78
8
8
W101810X-8270 .M Fri Oct 22 14:44:52 2010 EA# 0111-20 Page 91 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002879.D
18 Oct 2010 8:54 pm
dda
SSTD160-SCAN gcms22pg!25
gcmsprep2pg3 0
12 Sample Multiplier: 1
Quant Time Oct 20 12:15:17 2010
Quant Method : M:\ms2010\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 11:36:17 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
212
271
383
367
394
501
554
618
661
709
864
901
896
923
981
030
361
473
649
884
938
071
338
097
182
343
882
914
904
497
571
657
710
367
238
286
719
815
959
984
989
577
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
8115474
1651596
5451356
1061235
1347780
2126518
7226840
1923007
1852921
6077894
3463398
6120247
1667054
1373367
5463024
5335363
2194998
2292317
1449126
8396637
8383752
7617280
8883374
8829464
5525908
9167949
4110291
6289525
3588752
5599108
3194173
8682401
8108075
8822468
8302487
7385841
6925339
7257690
7263792
6422568m
5561636
4959425
148
169
155
231
194
173
151
185
176
153
165
156
180
208
159
149
166
167
199
147
147
154
140
149
181
144
162
176
158
161
188
156
155
160
175
160
164
167
160
183
153
141
51
86
98
92
65
27
67
88
96
16
04
39
27
84
98
99
50
65
06
82
81
63
95
38
15
39
28
56
87
26
73
97
61
90
99
30
78
35
48
55
67
74
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
94
76
93
100
74
87
91
98
81
96
99
99
91
8
98
100
96
90
89
96
96
97
98
95
98
94
100
100
100
96
98
96
97
100
98
93
100
96
3 a
[Manual Int. "WP" (EbE^
/
100
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Fri Oct 22 14:44:52 2010 EA# 0111-20 Page 92 of 126
Page: 2
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Min.
Max.
1 I
2
3
4 S
5 S
6
7
8
9
0
1
2
3
4
5
6
7
8
9 S
0
1 I
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6 I
7
8
9 S
0
1
2
3
4
5
6
7
8
9
0
1
2
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
1 , 4-Dichlorobenzene-d4 (I)
N-Nitrosodimethylamine
Pyridine
2-Fluorophenol (surr)
Phenol -d5 (surr)
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) ethe
3/4 -Methylphenol
N-Nitrso-di-n-propylamine (
Hexachloroe thane
Nitrobenzene-d5 (surr)
Nitrobenzene
Naphthalene-dS (I)
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2 -Chloroethoxy) methane
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylphenol (CC
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadiene (
Acenaphthene-dlO (I)
2, 4, 6-Trichlorophenol (CCC)
2,4, 5-Trichlorophenol
2-Fluorobiphenyl (surr)
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2 , 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
0
. 000
. 770
.281
.091
.268
.324
.599
. 782
.195
.371
.390
.645
.307
.876
. 966
. 148
.799
. 508
.245
.149
.000
. 584
.288
. 183
. 194
.356
.255
.336
.963
. 108
.202
.244
. 617
.597
.206
.000
.362
.363
.237
. 061
.274
.194
.204
.201
.291
. 136
. 609
.286
.029
.135
.204
.361
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
0
0
0
0
. 000
. 756
.260
.096
.246
.264
. 579
. 760
. 165
.319
.349
.639
.246
.830
. 942
. 097
. 773
.496
.221
.107
.000
. 587
.310
. 182
.218
.352
.258
.317
.944
. 109
. 196
.253
. 575
.587
.207
.000
.355
.366
.213
. 040
.277
.179
.182
.202
.289
. 136
. 602
.282
.994
.144
.208
.362
0 .
1 .
1.
-0.
1.
4.
3 .
2 .
2 .
3 .
2.
0.
4.
5.
2 .
4 .
3 .
2 .
1.
3.
0.
-0 .
-7 .
0 .
-12 .
1.
-1.
5.
2.
-0 .
3 .
-3 .
6 .
1.
-0.
0.
1 .
-0 .
1 .
2 .
-1.
7.
1.
-0.
0 .
0 .
0 .
1 .
3.
-6.
-2.
-0.
. 0
. 8
.6
.5
.7
.5
.3
. 8
. 5
. 8
.9
.9
.7
.3
. 5
.4
.3
.4
.9
.7
.0
. 5
. 6
. 5
.4
.1
.2
.7
.0
.9
. 0
. 7
. 8
.7
.5
.0
.9
. 8
.9
. 0
.1
.7
.8
.5
. 7
. 0
.4
.4
.4
.7
.0
.3
105
102
102
105
104
101
101
100
102
102
102
104
101
101
101
102
101
102
102
101
102
101
104
102
103
101
101
99
101
102
102
101
96
101
98
102
100
103
101
101
100
100
101
100
102
102
101
99
100
98
96
101
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
W101810X-8270 .M Mon Oct 25 09:59:33 2010 EA# 0111-20 Page 93 of 126
Page: 1
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Min.
Max.
53
54
55
56
57 S
58
59
60
61
62
63
64 I
65 S
66
67
68
69
70
71
72
73
74
75 S
76
77 S
78 I
79
80
81
82
83
84
85
86
87 I
88
89
90
91
92
93
94
95
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
Fluorene-dlO (surr)
4-Chlorophenyl-phenylether
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine (CCC
Azobenzene
Phenanthrene-dlO (I)
2 , 4 , 6-Tribromophenol (surr)
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene-dlO (surr)
Pyrene
Terphenyl-dl4 (surr)
Chrysene-dl2 (I)
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phthalate
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Perylene-dl2 (I)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
1
0
0
1
1
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
0
0
0
1
0
1
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
.403
.305
.308
.168
.098
.618
. 152
.272
. 194
. 005
.047
.000
.092
.210
.217
. 116
. 903
.902
.783
.002
.940
.485
. 834
. 009
. 664
.000
.442
.622
.394
. 606
.295
. 966
.910
.957
.000
. 000
. 977
. 891
. 920
.960
.794
. 767
.742
1
0
0
1
0
0
1
0
0
0
1
1
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
0
0
1
1
1
0
0
0
0
0
0
0
.338
.304
.308
.145
.983
.594
. 105
.272
.200
. 983
.020
.000
.097
.207
.215
. 122
. 885
.899
.792
.013
.907
.438
. 890
. 970
. 654
.000
.477
.625
.423
. 664
.294
. 956
.894
.048
.000
. 014
. 940
.916
.911
.928
.901
. 757
.738
4 .
0 .
0.
2.
10.
3.
4 .
0 .
-3 .
2 .
2.
0.
-5.
1 .
0 .
-5 .
2 .
0.
-1.
-1.
3.
9.
-6 .
3 .
1 .
0.
-7.
-0.
-7 .
-9.
0 .
1 .
1.
-9.
0.
-1 .
3 .
-2 .
1 .
3.
-13.
1.
0.
. 6
.3
.0
.0
.5
.9
. 1
. 0
. 1
.2
.6
.0
.4
.4
.9
.2
. 0
.3
.1
.1
.5
. 7
. 7
.9
. 5
.0
.9
.5
.4
. 6
.3
. 0
.8
.5
.0
.4
. 8
. 8
. 0
.3
.5
.3
.5
98
100
101
99
91
100
97
97
98
100
96
99
107
101
101
101
97
98
100
97
95
88
106
95
98
88
94
87
93
91
95
88
87
88
82
85
79
86
81
80
75
75
74
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
-0.
0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.01
.00
(#) = Out of Range
SPCC's out = 0 CCC's out = 0
W101810X-8270 .M Mon Oct 25 09:59:33 2010 EA# 0111-20 Page 94 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
8270C - Full List
Tue Oct 19 10:23:46 2010
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.15e+07
1.1e+07
TIC: W1002880.D\data.ms
500000
0
Time-> 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00 17.00 18.00
W101810X-8270 .M Mon Oct 25 09:59:17 2010EA# 0111-20 Page 95 of 126 Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5.
6 .
8 .
9.
12 .
14.
.375
. 588
.340
. 852
. 662
.890
152
136
164
188
240
264
688226
2399400
1346787
2321939
2016217
1770087
40
40
40
40
40
40
.00
. 00
. 00
. 00
. 00
.00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
-0 . 01
-0.01
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4.
4 .
5 .
7 .
8 .
9.
11.
11.
.243
.969
. 872
. 624
. 864
.136
.311
.423
112
99
82
172
176
330
212
244
942676
1072314
1050722
2042041
1654743
282026
2584320
1897215
50
49
49
49
44
53
53
49
.24
. 14
. 04
. 04
. 74
.05
.40
.25
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3 .
3 .
4 .
5.
5.
5.
5.
5 .
5 .
5 .
5 .
5.
5.
5.
5.
5 .
6 .
6 .
6 .
6.
6.
6.
6.
6 .
6 .
6 .
7 .
7.
7.
7.
7.
7 .
7 .
7 .
7 .
7.
8.
8.
8.
.249
.308
. 980
.065
.087
.188
.333
.391
.461
. 541
. 530
.573
.669
.696
.861
. 894
. 107
. 177
. 198
.241
.273
.422
.519
. 609
. 620
. 711
. 058
.282
.384
.437
.544
. 582
. 774
. 849
. 966
.998
.052
.073
.143
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
650462
1084039
1087452
497934
653516
1002204
1134739
1160863
550127
1071979
713985
810431
943342
665016
427064
952529
1759588
930823
547329
653741
1054912
773012
949915
2831328
326892
588090
757761
1723519
1760290
622246
598454
616336
1750104
466776
301474
1989983
339326
486482
229329
49
49
47
48
48
48
48
48
49
47
47
48
47
48
48
48
50
52
49
56
49
50
47
49
50
48
51
46
49
50
49
50
49
50
51
49
50
49
49
. 08
. 18
. 73
.32
.55
.75
.12
. 53
. 58
. 66
.36
.75
.78
.35
.84
.20
.23
.23
.91
.24
.45
.07
.10
. 00
.24
. 50
. 75
.57
.18
.26
.12
.43
. 00
. 66
.31
.07
.23
.65
.97
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
-0 . 01
-0 . 01
0 . 00
0 . 00
0.00
-0.01
0.00
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
89
83
92
90
89
95
99
99
88
95
96
82
95
71
88
87
96
67
90
1
97
96
97
87
73
99
93
100
99
100
99
92
94
86
85
99
77
75
88
W101810X-8270 .M Mon Oct 25 09:59:16 2010 EA# 0111-20 Page 96 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002880.D
18 Oct 2010 9:22 pm
dda
SSTD050-SCAN (ICV) gcms22pg!4
gcmsprep2pg!4
13 Sample Multiplier: 1
Quant Time Oct 20 12:15:59 2010
Quant Method : M:\ms2010\methods\wiley\W101810Xjunk-8270.M
Quant Title : 8270C - Full List
QLast Update : Tue Oct 19 10:23:46 2010
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2,3 -cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
11
11
11
12
12
12
12
13
14
14
14
14
14
16
16
17
207
260
378
356
378
484
543
613
655
698
858
896
880
907
971
019
355
467
644
879
927
066
333
086
172
332
871
904
898
491
555
646
694
362
222
265
703
794
938
957
957
550
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
2697323
475207
1672802
243039
349836
609727
2253212
511289
518904
1926868
1000592
1860174
457380
336568
1654790
1717854
601962
623183
354702
2568324
2608767
2298332
2940310
2633790
1271135
2814190
1202690
1576400
1066265
1673071
741563
2409352
2253864
2642075
2244455
2079980
2027457
2014918
2052550
1993996m
1674843
1633341
49
49
48
53
51
50
47
49
49
48
48
47
49
51
48
48
49
49
52
49
49
50
50
48
45
48
53
50
53
54
49
49
49
54
50
48
51
49
48
60
49
49
80
30
29
58
21
12
71
86
75
98
10
95
90
84
89
72
48
39
80
01
84
56
57
29
17
06
98
30
63
74
83
40
18
74
68
24
39
42
42
71
32
73
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
97
72
94
100
71
89
94
97
81
99
97
100
86
11
95
100
97
92
90
99
100
99
99
98
99
98
100
100
100
100
99
98
99
100
98
94
99
97
"[Manual Int. "WP" (EOE)|
96
99
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Oct 25 09:59:16 2010 EA# 0111-20 Page 97 of 126
Page: 2
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100157.D
21 Feb 2011 9:29 am
tdd
SSTD050 M8270 gcms22pg!49
gcmsprep2pg30 exp 3/15/11
2 Sample Multiplier: 1
Quant Time Feb 21 10:17:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Min.
Max.
1 I
2
3
4 S
5 S
6
7
8
9
0
1
2
3
4
5
6
7
8
9 S
0
1 I
2
3
4
5
6
7
8
9
0
1
2
3
4
5
6 I
7
8
9 S
0
1
2
3
4
5
6
7
8
9
0
1
2
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
1 , 4-Dichlorobenzene-d4 (I)
N-Nitrosodimethylamine
Pyridine
2-Fluorophenol (surr)
Phenol -d5 (surr)
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) ethe
3/4 -Methylphenol
N-Nitrso-di-n-propylamine (
Hexachloroe thane
Nitrobenzene-d5 (surr)
Nitrobenzene
Naphthalene-dS (I)
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2 -Chloroethoxy) methane
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylphenol (CC
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadiene (
Acenaphthene-dlO (I)
2, 4, 6-Trichlorophenol (CCC)
2,4, 5-Trichlorophenol
2-Fluorobiphenyl (surr)
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2 , 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
0
. 000
. 770
.281
.091
.268
.324
.599
. 782
.195
.371
.390
.645
.307
.876
. 966
. 148
.799
. 508
.245
.149
.000
. 584
.288
. 183
. 194
.356
.255
.336
.963
. 108
.202
.244
. 617
.597
.206
.000
.362
.363
.237
. 061
.274
.175
.204
.201
.291
. 136
. 609
.286
.029
.135
.204
.361
1
0
1
1
1
1
0
0
1
1
1
0
1
0
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
0
0
0
0
0
0
1
0
0
1
1
0
0
1
0
0
0
1
0
1
0
0
0
. 000
. 720
.287
.128
.357
.379
.619
. 775
.246
.446
.493
.721
.403
.977
.939
.199
. 781
. 545
.314
.182
.000
. 583
.344
.195
.199
.337
.282
.343
.033
. 110
.207
.286
. 675
.661
.190
.000
.359
.376
.249
. 062
.272
.185
.245
.207
.298
. 136
. 678
.285
.099
.133
.219
.385
0 .
6 .
-0.
-3.
-7.
-4.
-3 .
0 .
-4 .
-5 .
-7.
-11.
-7.
-11.
2 .
-4 .
2 .
-7 .
-5.
-2.
0.
0 .
-19.
-6 .
-2 .
5.
-10.
-2.
-7.
-1 .
-2 .
-17 .
-9.
-10.
7.
0.
0 .
-3 .
-1 .
-0 .
0.
-5.
-3.
-3.
-2 .
0 .
-4 .
0 .
-6.
1.
-7.
-6.
. 0
. 5
.5
.4
.0
.2
.3
.9
.3
. 5
.4
.8
.3
.5
. 8
.4
.3
.3
.5
.9
.0
.2
.4
. 6
. 6
.3
.6
.1
.3
.9
. 5
.2
.4
.7
.8
.0
. 8
. 6
. 0
. 1
.7
.7
.4
.0
.4
. 0
.3
.3
.8
.5
.4
.6
117
109
116
120
127
123
120
115
122
124
126
132
126
133
112
125
114
125
122
120
118
116
133
125
108
112
127
123
127
118
125
131
129
131
103
126
125
130
128
127
121
127
131
127
130
126
130
123
136
111
125
132
0 .
-0 .
-0.
-0.
-0.
0.
-0 .
-0 .
-0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
-0 .
-0 .
-0 .
-0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
-0 .
0 .
-0 .
0 .
-0.
0.
-0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
. 00
. 06
.05
.03
.02
.00
. 01
. 01
. 01
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.00
. 02
. 02
. 01
. 05
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.01
.01
.00
. 01
. 00
. 01
. 00
.01
.00
.01
.00
. 00
. 00
. 00
. 00
.00
.00
.00
.00
W101810X-8270 .M Mon Feb 21 10:19:02 2011 EA# 0111-20 Page 98 of 126
Page: 1
-------
Evaluate Continuing Calibration Report
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100157.D
21 Feb 2011 9:29 am
tdd
SSTD050 M8270 gcms22pg!49
gcmsprep2pg30 exp 3/15/11
2 Sample Multiplier: 1
Quant Time Feb 21 10:17:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Min.
Max.
53
54
55
56
57 S
58
59
60
61
62
63
64 I
65 S
66
67
68
69
70
71
72
73
74
75 S
76
77 S
78 I
79
80
81
82
83
84
85
86
87 I
88
89
90
91
92
93
94
95
RRF : 0.050 Min. Rel . Area : 50% Max. R.T. Dev O.SOmin
RRF Dev : 20% Max. Rel. Area : 200%
Compound AvgRF CCRF %Dev Area% Dev(min)
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
Fluorene-dlO (surr)
4-Chlorophenyl-phenylether
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine (CCC
Azobenzene
Phenanthrene-dlO (I)
2 , 4 , 6-Tribromophenol (surr)
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene-dlO (surr)
Pyrene
Terphenyl-dl4 (surr)
Chrysene-dl2 (I)
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phthalate
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Perylene-dl2 (I)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
1
0
0
1
1
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
0
0
0
1
0
1
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
.403
.305
.308
.168
.098
.618
. 152
.272
. 194
. 005
.047
.000
.092
.210
.217
. 116
. 903
.902
.783
.002
.940
.485
. 834
. 009
. 664
.000
.442
.622
.394
. 606
.295
. 966
.910
.957
.000
. 000
. 977
. 891
. 920
.960
.910
. 767
.742
1
0
0
1
1
0
1
0
0
1
1
1
0
0
0
0
0
0
0
1
1
0
0
1
0
1
0
0
0
0
0
0
0
1
1
1
1
0
0
0
0
0
0
.477
.313
.322
.330
.201
.666
.256
.287
.199
. 063
.077
.000
.101
.220
.228
. 122
.955
.957
.839
.162
.003
.479
. 870
. 090
. 709
.000
.505
.693
.453
. 691
.315
. 971
.905
.088
.000
. 078
. 072
. 925
. 986
.977
.817
.695
.642
-5 .
-2 .
-4.
-13.
-9.
-7.
-9.
-5 .
-2 .
-5 .
-2.
0.
-9.
-4 .
-5 .
-5 .
-5 .
-6.
-7.
-16.
-6.
1 .
-4 .
-8 .
-6 .
0.
-14.
-11.
-15 .
-14 .
-6 .
-0 .
0.
-13.
0.
-7 .
-9.
-3 .
-7 .
-1.
10.
9.
13.
.3
. 6
.5
.9
.4
.8
. 0
. 5
. 6
. 8
.9
.0
.8
. 8
. 1
.2
. 8
.1
.2
.0
.7
.2
.3
. 0
. 8
.0
.3
.4
. 0
. 0
. 8
. 5
.5
.7
.0
. 8
. 7
. 8
.2
.8
.2
.4
.5
133
128
130
142
137
138
136
126
119
133
125
129
146
140
140
131
137
135
138
144
137
125
135
140
139
131
146
142
147
140
150
132
130
134
108
120
120
115
117
112
90
91
86
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
0.
0.
0 .
0 .
0 .
0 .
0.
-0.
-0.
-0.
. 00
. 00
.00
.00
.00
.00
. 00
. 00
. 00
. 00
.01
.00
.00
. 00
. 00
. 00
. 00
.00
.00
.02
.01
. 01
. 01
. 00
. 02
.00
.02
.02
. 03
. 02
. 00
. 00
.00
.00
.00
. 00
. 00
. 00
. 00
.00
.05
.05
.07
(#) = Out of Range
SPCC's out = 0 CCC's out = 0
W101810X-8270 .M Mon Feb 21 10:19:02 2011 EA# 0111-20 Page 99 of 126
Page: 2
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100157.D
21 Feb 2011 9:29 am
tdd
SSTD050 M8270 gcms22pg!49
gcmsprep2pg30 exp 3/15/11
2 Sample Multiplier: 1
Quant Time Feb 21 10:17:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
8270C - Full List
Mon Feb 21 10:04:38 2011
Initial Calibration
Quant Title
QLast Update
Response via
Abundance
1.9e+07
1.8e+07
1.76+07
1.6e+07
1.5e+07
1.4e+07
1.36+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
TIC: W1100157.D\data.ms
' j
; [
»
f fe-
§ §5
It
>. i
o
o
o
1
3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00
W101810X-8270 .M Mon Feb 21 10:27:05 2 01lEA# 0111-20 Page 100 of 126
13.00 14.00 15.00 16.00
17.00 18.00
Page: 3
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100157.D
21 Feb 2011 9:29 am
tdd
SSTD050 M8270 gcms22pg!49
gcmsprep2pg30 exp 3/15/11
2 Sample Multiplier: 1
Quant Time Feb 21 10:17:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
Internal Standards
1)
21)
36)
64)
78)
87)
1 , 4-Dichlorobenzene-d4 . . .
Naphthalene-dS (I)
Acenaphthene-dlO (I)
Phenanthrene-dlO (I)
Chrysene-dl2 (I)
Perylene-dl2 (I)
5
6
8
9
12
14
247
460
212
724
502
660
152
136
164
188
240
264
768718
2761610
1657156
3022233
2979857
2352403
40
40
40
40
40
40
00
00
00
00
00
00
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
0.00
0 . 00
0 . 00
0 . 00
0 . 00
0.00
System Monitoring Compounds
4)
5)
19)
39)
57)
65)
75)
77)
2-Fluorophenol (surr)
Phenol -d5 (surr)
Nitrobenzene-d5 (surr)
2-Fluorobiphenyl (surr)
Fluorene-dlO (surr)
2,4, 6 -Tribromophenol ( . . .
Pyrene-dlO (surr)
Terphenyl-dl4 (surr)
4
4
5
7
8
9
11
11
115
847
749
496
736
008
183
295
112
99
82
172
176
330
212
244
1083905
1304316
1263006
2586297
2488349
383444
3284803
2679982
51
53
52
50
54
55
52
53
72
51
78
47
68
41
14
45
Target Compounds
2)
3)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
20)
22)
23)
24)
25)
26)
27)
28)
29)
30)
31)
32)
33)
34)
35)
37)
38)
40)
41)
42)
43)
44)
45)
46)
N-Nitrosodimethylamine
Pyridine
Phenol (CCC)
Aniline
bis (2-Chloroethyl) ether
2 - Chlorophenol
1 , 3 -Dichlorobenzene
1, 4-Dichlorobenzene (CCC)
Benzyl alcohol
1 , 2 -Dichlorobenzene
2 -Methylphenol
bis (2-Chloroisopropyl) . . .
3/4 -Methylphenol
N-Nitrso-di-n-propylam. . .
Hexachloroe thane
Nitrobenzene
Isophorone
2 , 4 -Dimethylphenol
2-Nitrophenol (CCC)
Benzoic acid
bis (2-Chloroethoxy) met . . .
2 , 4-Dichlorophenol (CCC)
1,2, 4 -Trichlorobenzene
Naphthalene
4 -Chloroaniline
Hexachlorobutadiene (CCC)
4-Chloro-3 methylpheno. . .
2 -Methylnaphthalene
1 -Methylnaphthalene
Hexachlorocyclopentadi . . .
2,4, 6 -Trichlorophenol . . .
2,4, 5 -Trichlorophenol
2-Chloronaphthalene
2-Nitroaniline
1 , 4 -Dinitrobenzene
Dimethylphthalate
1 , 3 -Dinitrobenzene
2 , 6 -Dinitrotoluene
1 , 2 -Dinitrobenzene
3
3
4
4
4
5
5
5
5
5
5
5
5
5
5
5
5
6
6
6
6
6
6
6
6
6
6
7
7
7
7
7
7
7
7
7
7
7
8
121
175
863
943
959
060
205
263
333
413
413
445
546
573
733
766
979
059
070
123
150
294
391
481
497
583
935
154
256
309
416
459
646
721
844
876
929
950
020
74
79
94
66
63
128
146
146
108
146
107
45
107
70
117
77
82
107
139
105
93
162
180
128
65
225
107
142
142
237
196
196
162
65
168
163
168
165
168
692191
1236732
1325043
595229
745136
1196912
1389344
1434640
692918
1347707
938657
901840
1151768
750418
523219
1135834
2013911
1186781
674759
685507
1163103
973055
1184682
3566902
378599
716020
986770
2329680
2280107
655259
744195
779670
2200285
563224
383071m
2579322
429040
618230
282621
46
50
52
51
49
52
52
53
55
53
55
48
52
48
53
51
49
59
53
51
47
55
51
53
50
51
58
54
55
45
49
51
50
49
52
51
51
51
50
76
23
07
72
56
12
74
69
91
65
73
56
23
85
57
46
95
66
46
23
37
25
04
63
55
31
54
70
35
98
63
85
06
68
97
69
62
28
05
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
-0.03
-0 . 02
0 . 00
-0 . 01
0 . 00
0.00
0.01
0.02
Qvalue
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
92
82
89
79
85
96
99
98
85
95
97
73
95
78
86
87
94
85
89
1
98
96
98
48
80
98
91
99
98
100
99
91
92
fManual Int. "WP" (TOC^
99
69
84
92
W101810X-8270 .M Mon Feb 21 10:27:04 2011 EA# 0111-20 Page 101 of 126
Page: 1
-------
Quantitation Report
(QT Reviewed)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100157.D
21 Feb 2011 9:29 am
tdd
SSTD050 M8270 gcms22pg!49
gcmsprep2pg30 exp 3/15/11
2 Sample Multiplier: 1
Quant Time Feb 21 10:17:15 2011
Quant Method : M:\ms2011\methods\wiley\W101810X-8270.M
Quant Title : 8270C - Full List
QLast Update : Mon Feb 21 10:04:38 2011
Response via : Initial Calibration
Compound
R.T. Qlon Response Cone Units Dev(Min)
47)
48)
49)
50)
51)
52)
53)
54)
55)
56)
58)
59)
60)
61)
62)
63)
66)
67)
68)
69)
70)
71)
72)
73)
74)
76)
79)
80)
81)
82)
83)
84)
85)
86)
88)
89)
90)
91)
92)
93)
94)
95)
Acenaphthylene
3-Nitroaniline
Acenaphthene (CCC)
2, 4-Dinitrophenol (SPCC)
4-Nitrophenol (SPCC)
2 , 4 -Dinitrotoluene
Dibenzofuran
2,3,5, 6 -Tetrachlorophenol
2,3,4, 6 -Tetrachlorophenol
Diethlyphthalate
4-Chlorophenyl-phenyle . . .
Fluorene
4-Nitroaniline
4 , 6 -Dinitro2 -methylphenol
N-Nitrosodiphenylamine . . .
Azobenzene
4-Bromophenyl-phenylether
Hexachlorobenzene
Pentachlorophenol (CCC)
Phenanthrene
Anthracene
Carbazole
Di-n-butylphthalate
Fluoranthene (CCC)
Benzidine
Pyrene
Butylbenzylphthalate
3 , 3 -Dimethylbenzidine
bis (2-ethylhexyl) adipate
bis (2-Ethylhexyl) phtha. . .
3,3' -Dichlorobenzidine
Benzo (a) anthracene
Chrysene
Di-n-octylphthalate (CCC)
Benzo (b) f luoranthene
Benzo (k) f luoranthene
Benzo (e) pyrene
Benzo (a) pyrene (CCC)
Perylene
Indeno (1,2, 3-cd) pyrene
Dibenz (a, h) anthracene
Benzo (g, h, i) perylene
8.
8.
8 .
8 .
8 .
8 .
8.
8.
8.
8.
8 .
8 .
8 .
8 .
8.
8.
9.
9.
9.
9.
9.
9.
10.
10.
11.
11.
11 .
11 .
11 .
12 .
12.
12.
12.
13.
14 .
14 .
14 .
14 .
14.
16.
16.
17.
.073
.132
.250
.234
.260
.362
.415
.485
.527
.576
. 730
. 768
. 757
. 784
.843
.896
.233
.339
. 516
. 745
.799
. 938
.216
.958
.044
.199
. 743
. 770
. 770
.347
.406
.486
.534
.191
. 014
. 057
.479
. 570
.708
.658
.664
.225
152
138
154
184
139
165
168
232
232
149
204
166
138
198
169
77
248
284
266
178
178
167
149
202
184
202
149
212
129
149
252
228
228
149
252
252
252
252
252
276
278
276
3475286
590339
2276836
274559
453573
797127
3058756
648895
666346
2755087
1379786
2602427
594855
412409
2202773
2230577
830116
861467
461878
3607123
3613835
3169488
4389808
3788528
1808735
4116044
1879649
2579977
1688696
2575456
1171876
3614992
3372090
4053141
3168592
3152928
2720643
2900409
2872198
2403082
2043957
1887142
52
49
53
49
53
53
52
51
52
56
53
54
52
51
52
51
52
52
52
52
53
53
57
53
49
53
57
55
57
57
53
50
49
56
53
54
51
53
50
44
45
43
.14
.78
.41
.20
. 71
.25
.63
.43
.18
.92
.91
. 52
. 74
.42
.89
.41
.42
.46
. 82
. 87
. 05
. 57
.99
.37
.37
.97
. 06
.69
.49
. 04
.24
.26
.76
.84
. 86
. 87
.91
. 62
.89
.92
.29
.25
ug/mL
ug/mL#
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL#
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
97
78
93
100
70
94
91
96
79
99
94
99
83
16
97
100
99
95
92
99
99
99
99
100
100
98
100
100
100
98
99
98
99
100
99
95
100
98
100
95
98
98
(#) = qualifier out of range (m) = manual integration (+) = signals summed
W101810X-8270 .M Mon Feb 21 10:27:04 2011 EA# 0111-20 Page 102 of 126
Page: 2
-------
DFTPP
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Integration File: rteint.p
Method : M:\ms2010\methods\wiley\W101810X-8270.M
Title : 8270C - Full List
Last Update : Wed Oct 20 14:58:44 2010
Abundance
4000000
3000000
2000000
1000000
0
Time~>
Abundance
300000
250000
200000
150000
100000
50000
n
8.20
TIC: W1002872.D\data.ms
8.40
8.60 8.80
77.1
51
.1
i ii ,
III
107
.1
9.00 9.20 9.40 9.6C
Average of 10.0
19
127
M
148.! 167.1^
— I — I — I — I — 1 —
) 9.80
23 to 1 0
3.0
22
A . II 11.359 11.J01 I
i — i — i — f — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — \ — i — r • r i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i — i
10.00 10.20 10.40 10.60 10.80 11.00 11.20 11.40 11.60 11.80
034 min.: W1002872.D\data.ms (-)
442.1
255.1
275.1
4.1
I'lii.i.
296.1
..„ |, , ,„ , , 323-1, 346.0 365-°383.1 403.1 423/1
m/z~>
40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440
AutoFind: Scans 1318, 1319, 1320; Background Corrected with Scan 1312
Target
Mass
51
68
69
70
127
197
198
199
275
365
441
442
443
Rel. to
Mass
198
69
198
69
198
198
198
198
198
198
443
198
442
Lower
Limit%
30
0 . 00
0 . 00
0 . 00
40
0.00
100
5
10
1
0 . 01
40
17
Upper
Limit%
60
2
100
2
60
1
100
9
30
100
100
100
23
Rel.
Abn%
30.0
0 . 0
38 . 9
0 . 6
47 .2
0.0
100.0
7.1
27.9
4 . 1
77 . 9
77 . 1
19.1
Raw
Abn
92083
0
119435
751
144776
0
306901
21658
85621
12456
35283
236523
45283
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
W101810X-8270 .M Mon Oct 25 10:18:03 2010EA# 0111-20 Page 103 of 126
Page: 1
-------
Average of 10.023 to
DFTPP
Modified:subtracted
10.034 min.: W1002872.D\data.ms
m/z
35 . 10
36 . 05
37 . 00
38 . 10
39.15
40.10
41.15
45.00
45 .20
48 . 10
49 . 10
Average of
DFTPP
abund .
71
140
389
1201
7406
153
124
193
44
47
588
10.023 to
m/z
50 . 10
51 . 10
52 . 15
53 . 10
55.10
56.10
57.10
58.10
58 . 90
60 . 00
61 . 10
10.034 min.
abund .
25623
92083
4613
249
668
3048
6631
250
78
124
1482
m/z
62 . 10
63 . 10
64 . 05
65 . 10
65.80
66.15
67.10
69.10
70 . 10
71 . 10
72 . 00
. : W1002872 .D\data.
abund .
1589
4232
599
2029
55
120
204
119435
751
58
114
. ms
m/z
73 . 10
74 . 10
75 . 10
76 . 15
77.10
78.10
79.10
80.10
81 . 10
82 . 10
83 . 10
Modified : subtracted
m/z
83.85
84 . 10
84 .30
85 . 10
86 . 10
87.05
88.05
89.00
89.90
90 .30
91 . 10
Average of
DFTPP
abund .
99
126
116
1840
2620
1311
367
278
35
47
2159
10.023 to
m/z
92.10
93 . 10
94 . 05
95 . 05
96 . 15
98.10
99.10
100.10
101.05
101 . 90
103 . 10
10.034 min.
abund .
2468
15303
1071
347
586
11724
9039
873
4787
341
1964
m/z
104.10
105 . 10
106 . 00
107 . 10
108 . 10
110.10
111.10
112.10
113.00
113 . 15
114 . 80
. : W1002872 .D\data.
abund .
3541
2975
177
39725
5856
70621
11083
1404
177
216
78
. ms
m/z
115.00
116 . 10
117 . 10
118 . 10
119 . 10
120.05
121.00
122.05
123.10
124 . 10
125 . 10
Modified : subtracted
m/z
127.10
128.10
129 . 10
130 . 05
131 . 05
132 . 00
133.05
134.05
135.10
136.10
137 . 10
Average of
DFTPP
abund .
144776
11349
58907
4828
930
396
394
1606
4589
1650
2650
10.023 to
m/z
137.80
138.20
139 . 00
140 . 00
141 . 10
142 . 00
143.05
144.15
145.00
146.05
147 . 05
10.034 min.
abund .
464
83
289
732
7532
2457
1791
470
328
1588
3771
m/z
148.10
149.10
150 . 05
151 . 10
151 . 90
152 .20
153.00
154.10
155.10
156.10
157 . 05
. : W1002872 .D\data.
abund .
8660
1984
661
997
313
399
2621
1608
4353
6416
1396
. ms
m/z
158.05
159.05
160 . 05
161 . 10
162 . 00
163 . 10
164.10
165.05
166.10
167.10
168 . 05
Modified : subtracted
m/z
169.10
170.05
170.40
171 . 00
172 . 10
173 . 10
174 . 05
175.10
176.10
177.00
178.05
Average of
DFTPP
abund .
1368
566
216
688
1354
2000
3320
5899
1852
3035
753
10.023 to
m/z
179.00
180.10
181.10
182 . 00
182 . 90
184 . 05
185 . 10
186.10
187.05
188.05
189.05
10.034 min.
abund .
11587
7881
3852
592
349
828
5521
43536
12341
1310
2902
m/z
190.10
191.05
192.10
193 . 10
194 . 00
195 . 00
196 . 10
198.00
199.00
200.00
201.55
. : W1002872 .D\data.
abund .
527
1274
3773
4234
954
402
10417
306901
21658
1542
1992
. ms
m/z
203.05
204.10
205.10
206 . 10
207 . 10
208 . 05
209 . 05
210.05
211.10
211.90
213.05
Modified : subtracted
m/z
214.20
215.00
216.10
217.00
218 . 00
219 .20
221 . 10
221 . 90
223.00
224.10
225.10
Average of
abund .
58
938
1914
22667
2506
250
18122
1255
5264
48035
11551
10.023 to
m/z
226.10
227.05
228.00
229.05
229 . 95
231 . 10
231 . 90
232 . 10
233.00
233.20
234.05
10.034 min.
abund .
1311
20096
2934
4092
612
1744
74
288
246
173
1356
m/z
235.10
236.10
237.10
237.90
238 . 10
239 . 00
240 . 05
241 . 00
242.05
243.10
244.10
. : W1002872.D\data.
abund .
1361
924
1915
146
116
965
723
1150
2715
2626
36661
. ms
m/z
245.10
246.00
247.05
248.05
249 . 00
250 . 00
251 . 05
252 . 10
253.10
255.10
256.10
abund.
1095
12303
18631
6251
123147
8219
8921
7354
9291
2334
1632
abund.
47
1081
33520
2531
243
477
228
3061
4126
2074
1728
abund.
1390
1023
2349
3680
1057
254
412
2639
2444
15701
7359
abund.
2362
11774
20593
83491
11545
2714
934
806
3361
95
233
abund.
4908
7769
1547
339
1384
235
299
358
876
190088
27339
W101810X-8270 .M Mon Oct 25 10:18:03 2010 EA# 0111-20 Page 104 of 126
Page: 2
-------
DFTPP
Modified:subtracted
m/z
257.10
258 . 00
259 . 00
260 . 00
260 . 95
262.20
262.90
264.05
265.00
265 . 90
267 . 00
abund .
2400
11841
2008
312
301
50
120
363
4967
720
247
Average of 10.023 to
DFTPP
Modified:
m/z
302.00
303.10
304 . 05
304 . 90
305 . 15
306 . 00
306.95
308.05
308.70
309.00
309 .20
subtracted
abund .
466
2920
898
75
99
48
100
391
39
254
58
Average of 10.023 to
DFTPP
Modified:
m/z
343.00
343.30
346.00
347 . 05
350 . 10
351 . 15
352 . 05
353.00
354.10
355.00
358.00
subtracted
abund .
55
43
1783
370
123
93
2544
1802
2480
455
50
Average of 10.023 to
DFTPP
Modified:
m/z
415.05
416.90
420.00
421.05
422 . 10
423 . 10
424 . 05
425 . 05
426.10
427.10
431.70
subtracted
abund .
141
38
95
1802
1923
12408
3115
396
39
53
60
m/z
267.90
268 . 90
269 . 70
269 . 95
271 . 00
272.00
273.10
274.05
275.10
276 . 05
277 . 05
10.034 min.
m/z
310.05
311.10
312 . 00
312 .20
312 . 90
313 . 05
314.00
315.05
316.10
317.10
320 . 00
10.034 min.
m/z
358.90
359.15
360.80
363 . 70
365 . 00
366 . 05
367 . 10
370.05
371.10
372.05
373.10
10.034 min.
m/z
434.10
434.40
436.80
437.30
437 . 80
438 .45
439 .20
439 . 70
441.10
442.10
443.10
abund .
132
37
68
225
445
304
5792
15820
85621
11521
7677
m/z
278.05
279 . 05
281 . 00
282 . 10
283 . 05
284.05
285.10
286.00
288.10
288 . 95
290 . 05
. : W1002872 .D\data.
abund .
370
49
47
42
81
244
1443
2901
1693
366
144
m/z
321.05
321.95
323 . 10
324 . 10
324 . 90
325 .20
325.95
326.20
326.95
328.10
329 . 00
. : W1002872 .D\data.
abund .
57
117
45
51
12456
1888
40
184
558
4186
1033
m/z
373.90
374.30
376.90
377 . 10
383 . 05
383 . 95
385 . 00
389.95
391.10
392.00
393.30
. : W1002872 .D\data.
abund .
40
37
73
83
52
262
61
231
35283
236523
45283
m/z
444.10
444.80
445.10
445.30
abund .
1203
284
36
361
899
699
1520
314
116
342
214
. ms
abund .
880
225
8598
1553
84
55
97
62
1558
913
132
. ms
abund .
90
57
63
35
1081
400
48
614
389
311
41
. ms
abund .
4210
61
190
34
m/z
291.05
291 . 90
292 . 10
293 . 05
294 . 00
296.05
297.10
298.10
299.70
300 . 85
301 . 10
m/z
332.00
333.05
334 . 05
335 . 05
335 . 90
336 .20
339.10
339.80
340.20
341.10
342 . 10
m/z
395.00
395.20
396.70
400 . 90
401 . 15
402 . 05
403 . 05
404.05
405.05
410.00
410.20
m/z
abund .
291
117
257
1842
360
26536
3759
176
38
165
147
abund .
593
840
5405
1336
42
127
45
67
61
1038
351
abund .
45
43
34
75
140
1261
2245
743
159
82
38
abund .
W101810X-8270 .M Mon Oct 25 10:18:03 2010 EA# 0111-20 Page 105 of 126
Page: 3
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Quant Time Oct 25 10:14:04 2010
Quant Method : M:\ms2010\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Fri Sep 17 17:54:07 2010
Response via : Initial Calibration
Abundance
400000
300000
200000
100000
Ion 265.90 (265.60 to 266.60): W1002872.D\data.ms
Ion 267.90 (267.60 to 268.60): W1002872.D\data.ms
Ion 263.90 (263.60 to 264.60): W1002872.D\data.ms
9.644 Tailing = 1.09
Time-> 9.48 9.50 9.52 9.54 9.56 9.58 9.60 9.62 9.64 9.66 9.68 9.70 9.72 9.74 9.76 9.78 9.80 9.82 9.84 9.86 9.88 9.90
Abundance Scan 1247 (9.644 min): W1002872.D\data.ms
26^.9
300000]
250000
200000
150000
100000
50000
165.0
95.1
60.1
47.1
130.0
,108.0
143.0
201.9
178.9
229.9
215.9
.,i 842.8
281.1
356.2
30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360
TIC: W1002872.D\data.ms
(1) Pentachlorophenol
9.644min (-0.101) 51.44ug/mL
response 314663
Ion Exp% Act%
265.90 100 100
267.90 65.10 62.69
263.90 65.90 61.44
0.00 0.00 0.00
DFTPP-4-EA.M Mon Oct 25 10:14:30 2010 EA# 0111-20 Page 106 of 126
Page: 1
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2010Q4\wiley\data\octlO\W101810A\
W1002872.D
18 Oct 2010 5:33 pm
dda
DFTPP
1 Sample Multiplier: 1
Quant Time Oct 25 10:14:04 2010
Quant Method : M:\ms2010\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Fri Sep 17 17:54:07 2010
Response via : Initial Calibration
Abundance
1400000
1200000
1000000
800000
600000
400000
200000
0
Ion 184.20 (183.90to 184.90): W1 002872. D\data.ms
Ion 185.20(184.90(0 185.90): W1 002872. D\data.ms
Ion 183.10(182.80(0 183.80): W1 002872. D\data.ms
11.172 Tailing = 0.90 |
A
\
'
'
J \ '
1/XV_ i
Time-> 11.00 11.02 11.04 11.06 11.08 11.10 11.12 11.14 11.16 11.18 11.20 11.22 11.24 11.26 11.28 11.30 11.32 11.34 11.36
Abundance Scan 1533 (11.172 min): W1002872.D\data.ms
1200000 j 18^-2
1000000
800000
600000
400000
200000
92.1
391
156.2
207.1
239.0
281.1
341.2
461.1
m/z-->
40 60
80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400
TIC: W1002872.D\data.ms
420 440 460
(3) Benzidine
11.172min (-0.101) 48.65 ug/mL
response 1263024
Ion Exp% Act%
184.20 100 100
185.20 13.60 14.18
183.10 12.20 11.72
0.00 0.00 0.00
DFTPP-4-EA.M Mon Oct 25 10:15:21 2010 EA# 0111-20 Page 107 of 126
Page: 1
-------
File
Operator
Acquired
Instrument
Sample Name
Misc Info
Vial Number
M:\ms2010Q4\wiley\data\octlO\W101810A\W1002872.D
dda
18 Oct 2010 5:33 pm using AcqMethod RXI-2A-EA.M
Wiley
DFTPP
Abundance
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
Time~>
TIC: W1002872.D\data.ms
D>D>T Breakdown / Degradation = 3.6%
32
9.60 9.80 10.00 10.20
10.40 10.60 10.80 11.00 11.20 11.40
EA# 0111-20 Page 108 of 126
11.60 11.80 12.00 12.20
-------
TIC: Wl002872.D\data.ms
DFTPP
Peak # Ret Time Type Width Area Start Time End Time
1 11.359 rm 0.032 11072 11.343 11.375
2 11.701 rm 0.102 161495 11.674 11.775
3 12.032 rm 0.080 4671955 12.000 12.080
EA# 0111-20 Page 109 of 126
-------
DFTPP
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100156.D
21 Feb 2011 8:58 am
tdd
DFTPP
1 Sample Multiplier: 1
Integration File: rteint.p
Method : M:\ms2011\methods\wiley\W101810X-8270.M
Title : 8270C - Full List
Last Update : Wed Oct 20 14:58:44 2010
Abundance
5000000
4000000
3000000
2000000
1000000
TIC: W1100156.D\data.ms
A
Time-> 8.00 8.20 8.40 8.60 8.80 9.00 9.20 9.40 9.60 9.80 10.00 10.20 10.40 10.60 10.80 11.00 11.20 11.40 11.60 11.80
Abundance Average of 9.900 to 9.911 min.: W1100156.D\data.ms (-)
198
400000
300000
200000
100000
127
77
51
39
63
110
93
m/z~>
148 167179
442
255
224
243
275
296
310323335 3523^5 383 403
IH—... , ill. ,. l| 'I H-k ,.. , i., IJ-, ,— I , I , ,—H .—, , H-L, , ,
40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460
AutoFind: Scans 1295, 1296, 1297; Background Corrected with Scan 1288
Target
Mass
51
68
69
70
127
197
198
199
275
365
441
442
443
Rel. to
Mass
198
69
198
69
198
198
198
198
198
198
443
198
442
Lower
Limit%
30
0 . 00
0 . 00
0 . 00
40
0.00
100
5
10
1
0 . 01
40
17
Upper
Limit%
60
2
100
2
60
1
100
9
30
100
100
100
23
Rel.
Abn%
30.6
0 . 0
37 . 8
0 .4
47 . 7
0.0
100.0
6.8
28.9
4 .3
77 .4
82 . 5
19 .2
Raw
Abn
135289
0
167234
752
210976
0
442304
30104
127659
18953
54120
364736
69893
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
W101810X-8270 .M Mon Feb 21 10:20:39 201lEA# 0111-20 Page 110 of 126
Page: 1
-------
Average of 9.900 to 9
DFTPP
Modified:subtracted
.911 min.: W1100156.D\data.ms
m/z
35 . 50
36 . 00
37 . 10
38 .20
39.10
40.05
41.10
42.00
44 . 05
45 . 05
47 . 05
Average of <
DFTPP
abund .
37
96
866
2199
11173
646
190
13
401
224
126
3 . 900 to E
m/z
48 .25
50 . 10
51 . 05
52 . 10
53.10
54.15
55.10
56.10
57 . 10
58 . 00
58 . 95
3.911 min.
abund .
119
38695
135289
7149
335
106
1320
4260
9842
357
53
: W1100156
m/z
60 . 05
61 . 10
62 . 15
63 . 10
64.15
65.10
66.15
67.10
69 . 10
70 . 10
71 . 10
. D\data .ms
abund .
51
1990
2071
6346
824
2899
320
64
167234
752
91
m/z
71 . 80
72 . 10
73 . 05
74 . 10
75.05
76.15
77.10
78.10
79 . 10
80 . 10
81 . 05
abund .
121
50
1229
17539
25465
8670
170299
11230
12912
9631
13548
Modified : subtracted
m/z
82.05
83 . 05
84 . 10
85 . 10
86 . 05
87.10
88.05
88.95
90.00
91 . 05
92 . 10
Average of <
DFTPP
abund .
3333
2578
523
2392
3537
1867
770
381
47
3256
3883
3 . 900 to E
m/z
93.05
94 . 10
95 . 00
96 . 05
97 . 05
98.10
99.10
100.05
101.05
101 . 95
103 . 05
3.911 min.
abund .
22963
1485
317
990
326
17843
11955
1062
7466
344
2189
: W1100156
m/z
104.10
105 . 05
106 . 10
107 . 10
108 . 05
110.10
111.10
112.05
113.05
113 . 80
114 . 00
. D\data .ms
abund .
4943
4704
790
58411
8829
101530
16784
2141
650
75
61
m/z
115.00
116 . 10
117 . 05
118 . 00
119 . 00
120.10
121.00
122.00
123.10
124 . 10
125 . 05
abund .
241
3972
50844
3600
387
665
317
4170
6187
2899
3015
Modified : subtracted
m/z
127.10
128.05
129 . 05
130 . 00
131 . 00
132 . 05
132.90
133.10
134.00
135.10
136 . 10
Average of <
DFTPP
abund .
210976
16994
85093
7120
1359
720
50
377
2474
7176
2942
3 . 900 to E
m/z
137.05
137.95
139 . 05
140 . 05
141 . 00
142 . 00
143.00
144.15
145.00
146.05
147 . 05
3.911 min.
abund .
3183
345
479
1367
12327
4058
2191
534
519
2126
6063
: W1100156
m/z
148.05
149.05
150 . 00
151 . 05
151 . 75
152 . 10
153.00
154.00
155.00
156.10
157 . 10
. D\data .ms
abund .
13163
2486
786
1461
674
167
4001
2700
6536
9194
1481
m/z
157.30
157.90
159 . 00
160 . 10
161 . 05
162 . 05
163.00
164.10
165.05
166.00
167 . 00
abund .
276
2200
1790
3812
5049
1580
440
752
4747
3364
21566
Modified : subtracted
m/z
168.00
169.05
170.05
171 . 10
172 . 00
173 . 10
174 . 05
175.10
176.00
177.10
178.00
Average of <
DFTPP
abund .
10604
1541
893
753
2038
2664
4814
8701
2581
4013
629
3 . 900 to E
m/z
178.20
179.00
180.05
181 . 05
182 . 10
182 . 75
184 . 10
185.10
186.10
187.00
188.10
3.911 min.
abund .
732
17350
11538
5628
902
536
1404
7805
63797
18888
1717
: W1100156
m/z
189.00
190.05
191.00
192 . 00
193 . 00
194 . 05
195 . 10
196.00
198.00
199.00
200.00
. D\data .ms
abund .
4435
723
1575
5101
6230
1357
186
13759
442304
30104
2520
m/z
201.55
203.05
204.00
205 . 05
206 . 10
207 . 10
208 . 00
209.00
210.10
211.00
212.00
abund .
2313
3173
16987
28933
117456
16170
3817
1454
2115
4905
203
Modified : subtracted
m/z
213.10
213.70
214.10
214.95
216 . 05
217 . 00
218 . 00
219 . 05
221.05
223.00
224.00
Average of <
abund .
443
46
96
1597
2600
32536
4277
560
23882
7526
69528
3.900 to E
m/z
225.10
226.10
227.00
228.00
229 . 00
229 . 95
231 . 10
232 . 00
232.95
234.00
235.10
3.911 min.
abund .
17544
1230
31309
4514
5629
921
2605
364
607
2028
1985
: W1100156
m/z
236.00
237.05
238.10
239.05
240 . 05
241 . 10
242 . 00
243 . 10
244.10
245.05
246.00
. D\data .ms
abund .
1392
2296
329
1358
921
1649
3623
3634
54584
7026
11163
m/z
247.00
247.90
248.05
248.30
249 . 00
249 . 80
250 . 05
251 . 00
252.05
253.05
255.00
abund .
2666
171
542
165
1983
53
297
603
555
1391
286763
W101810X-8270 .M Mon Feb 21 10:20:39 2011 EA# 0111-20 Page 111 of 126
Page: 2
-------
DFTPP
Modified:subtracted
m/z
256.00
257 . 05
258 . 00
259 . 00
259 . 95
261.05
263.00
263.90
265.00
265 . 90
267 . 10
abund .
41949
3005
17498
2525
446
451
180
370
7414
1073
268
Average of 9.900 to E
DFTPP
Modified:
m/z
298.05
298.80
299 . 15
300 . 90
301 . 05
301 . 95
303.10
304.00
305.00
305.15
306 . 00
subtracted
abund .
397
35
99
229
446
973
4419
1220
64
120
96
Average of 9.900 to S
DFTPP
Modified:
m/z
335.90
339.00
340.05
341 . 05
342 . 00
342 . 90
344 .20
345.00
346.00
346.95
347.80
subtracted
abund .
299
191
320
1181
503
51
43
41
2679
610
59
Average of 9.900 to E
DFTPP
Modified:
m/z
396.60
397.20
401.05
402.00
403 . 05
404 . 00
404 . 85
405 . 10
406.10
409.10
410.10
subtracted
abund .
47
46
400
2466
3541
955
186
70
38
33
68
m/z
270.00
271 . 05
272 . 00
273 . 00
274 . 00
275.00
276.00
277.00
277.90
278 . 10
279 . 00
3.911 min.
m/z
307.30
307.90
309 . 00
310 . 05
310 . 95
312 . 00
312.80
313.00
314.00
315.00
316 . 10
3.911 min.
m/z
349.50
350.90
352.05
353 . 05
354 . 05
355 . 00
356 . 90
357.20
359.00
360.10
362.10
3.911 min.
m/z
415.00
415.20
418.20
419.70
421 . 00
422 . 05
423 . 00
424 . 05
425.05
427.00
429.40
abund .
440
634
753
8905
23283
127659
16703
11042
601
1220
402
: W1100156
abund .
90
751
473
523
108
174
208
179
2204
4602
2593
: W1100156
abund .
37
203
3693
2409
4037
852
42
36
232
49
45
: W1100156
abund .
113
71
37
79
2923
2736
20225
3835
589
47
58
m/z
281.00
282 . 05
283 . 00
283 . 90
284 . 10
285.05
285.90
286.10
286.90
287 . 70
288 . 05
. D\data . ms
m/z
317.00
318.10
318 . 90
319 . 10
319 . 90
320 .20
321.00
321.20
321.90
322.10
323 . 00
. D\data .ms
m/z
363.10
365.00
366.00
366 . 80
367 . 00
367 . 90
370 . 00
371.15
372.00
373.05
373.90
. D\data .ms
m/z
430.10
431.40
432.80
433.60
434 . 00
434 . 80
436 .40
436 . 80
437.10
437.55
438.05
abund .
228
240
1545
617
257
2083
67
296
43
47
122
abund .
481
44
62
39
131
57
825
375
240
332
11881
abund .
64
18953
2550
64
78
71
384
649
6054
1617
278
abund .
35
34
48
35
41
64
74
48
87
151
136
m/z
288.95
289 . 70
289 . 95
290 . 85
291 . 80
292.10
293.05
293.90
294.10
296 . 00
297 . 00
m/z
324.05
325.00
326 . 05
326 . 95
327 . 95
329 . 05
330.20
332.00
333.05
334.10
335 . 00
m/z
377.10
381.90
383.05
384 . 00
385 . 05
386 . 00
388 . 80
389.95
391.05
392.05
394.90
m/z
438.40
438.80
439.10
441.05
442 . 00
443 . 05
444 . 00
445 . 00
445.90
448.10
451.20
abund .
571
98
333
208
144
443
2254
182
528
40261
5607
abund .
2242
233
264
2290
1397
258
72
853
1237
8048
1940
abund .
123
45
1906
387
167
69
53
761
544
402
90
abund .
119
112
347
54120
364736
69893
6381
536
39
46
37
W101810X-8270 .M Mon Feb 21 10:20:39 2011 EA# 0111-20 Page 112 of 126
Page: 3
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100156.D
21 Feb 2011 8:58 am
tdd
DFTPP
1 Sample Multiplier: 1
Quant Time Feb 21 09:21:42 2011
Quant Method : M:\ms2011\methods\wiley\DFTPP-4-EA.M
Quant Title : DFTPP
QLast Update : Thu Dec 30 11:51:21 2010
Response via : Initial Calibration
Abundance
500000
Ion 265.90 (265.60 to 266.60): W1100156.D\data.ms
400000
300000
200000
100000
0
Time~>
Abundance
300000
250000
200000
150000
100000
50000
0
m/z-->
9.41 9.42 9.43
9.44 9.45
J
9.515
A
\
\
\
/ \
\
/ \
V
1
Tailing =
1_
0.93
9.46 9.47 9.48 9.49 9.50 9.51 9.52 9.53 9.54 9.55 9.56 9.57 9.58 9.59 9.60 9.61 9.62 9.63 9.64 9.65 9.66 9.67
Scan 1223 (9.515 min): W1 100156.D\datc
2f
165
6
36 47
, . ,
40
6
0
71 83
0
80
9
i
I,
1C
107H8
I, Jill ,.i
o 1;
13
10
0
143
III, 153,.
140 160
2(
|, 179 191
180 20
)2
2
1,214
0 220
30
,,|241251
.ms
i6
|, 281 341 402
240 260 280 300 320 340 360 380 400
TIC: W1100156.D\data.ms
(1) Pentachlorophenol
9.515min (-0.065) 68.77 ug/mL
response 347630
Ion Exp% Act%
265.90 100 100
267.90 65.10 65.31
263.90 65.90 63.17
0.00 0.00 0.00
DFTPP-4-EA.M Mon Feb 21 10:29:03 2011 EA# 0111-20 Page 113 of 126
Page: 1
-------
Quantitation Report (Qedit)
Data Path
Data File
Acq On
Operator
Sample
Misc
ALS Vial
M:\ms2011ql\wiley\data\febll\W022111A\
W1100156.D
21 Feb 2011 8:58 am
tdd
DFTPP
1 Sample Multiplier: 1
Quant Time Feb 21 09:21:42 2011
Quant Method
Quant Title
QLast Update
Response via
Abundance
1500000
1 000000
500000
M:\ms2011\methods\wiley\DFTPP-4-EA.M
DFTPP
Thu Dec 30 11:51:21 2010
Initial Calibration
Ion 184.20(183.90(0 184.90): W1100156.D\data.ms
11.043 Tailing = 0.97
,
Time~> 10.94
Abundance
1200000
1000000
800000
600000
400000
200000
10.96 10.98
11.00 11.02 11.04 11.06 11.08 11.10 11.12 11.14
Scan 1509 (11.043 min): W1100156.D\data.ms
1$4
11.16 11.18
11.20
11.22
39 52
77
77
1Q3
117 130
117
156
167
196207 221 239 257
281
312 327 341 355
402
430 446
40
60 80 100 120 140 160 180
200 220 240 260 280 300
TIC: W1100156.D\data.ms
320 340 360 380 400 420 440
(3) Benzidine
11.043min (-0.064) 84.40 ug/mL
response 1373380
Ion Exp% Act%
184.20 100 100
185.20 13.60 14.16
183.10 12.20 11.61
0.00 0.00 0.00
DFTPP-4-EA.M Mon Feb 21 10:29:27 2011 EA# 0111-20 Page 114 of 126
Page: 1
-------
File
Operator
Acquired
Instrument
Sample Name
Misc Info
Vial Number
M:\ms2011ql\wiley\data\febll\W022111A\W1100156.D
tdd
21 Feb 2011 8:58 am using AcqMethod RXI-2A-EA.M
Wiley
DFTPP
Abundance
5400000
5200000
5000000
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
Time~>
TIC:W1100156.D\data.ms
D>D>T Breakdown / Degradation = 6.9%
9.60 9.80 10.00 10.20 10.40 10.60 10.80
EA# 0111-20 Page 115 of 126
11.00
11.20
11.40
11.60
11.80
-------
TIC: Wll00156.D\data.ms
DFTPP
Peak # Ret Time Type Width Area Start Time End Time
1 11.230 rm 0.048 24291 11.209 11.257
2 11.572 rm 0.102 374417 11.535 11.636
3 11.887 rm 0.064 5420668 11.861 11.925
EA# 0111-20 Page 116 of 126
-------
Sequence Name: M:\ms2010q4\wiley\sequence\w101810a
Operator: ede
Datapath: C:\ms2010q4\wiley\data\oct10\w101810a
Line Type
Vial DataFile
Method
Sample Name
Additional Info
Comments
1 DFTPP
2 DFTPP
3 DFTPP
4 Calibration
5 Sample
6 Blank
7 Calibration
8 Calibration
9 Calibration
10 DFTPP
11 Calibration
12 Calibration
13 Calibration
14 Calibration
15 Calibration
16 Calibration
17 Calibration
18 Calibration
19 Blank
20 Sample
21 Sample
22 Sample
23 Sample
1
1
1
2
4
5
2
2
2
1
6
7
8
9
10
11
12
13
2
16
17
18
19
W1 002863
W1 002864
W1 002865
W1 002866
W1 002867
W1 002868
W1 002869
W1 002870
W1 002871
W1 002872
W1 002873
W1 002874
W1 002875
W1 002876
W1 002877
W1 002878
W1 002879
W1 002880
W1 002881
W1 002882
W1 002883
W1 002884
W1 002885
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
RXI-2A-EA
DFTPP
DFTPP
DFTPP
SSTD005-SCAN
High Cone Mega Mix
CH2CI2 System Blank
SSTD005-SCAN
SSTD005-SCAN
SSTD005-SCAN
DFTPP
SSTD005-SCAN
SSTD010-SCAN
SSTD020-SCAN
SSTD050-SCAN
SSTD080-SCAN
SSTD120-SCAN
SSTD160-SCAN
SSTD050-SCAN (ICV)
CH2CI2 System Blank
IDOC EDE "A"
IDOC EDE "B"
IDOC EDE "C"
IDOC EDE "D"
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg30
gcmsprep2pg14
QA-BS-1pg72 M8270-BS "A"
QA-BS-1pg72 M8270-BS "B"
QA-BS-1pg72 M8270-BS "C"
QA-BS-1pg72 M8270-BS "D"
EA# 0111-20 Page 117 of 126
-------
Sequence Name: M:\ms2011q1\wiley\sequence\w022111a
Operator: tdd
Datapath: C:\ms2011q1\wiley\data\feb11\w022111a
Line Type Vial DataFile
Method
Sample Name
Additional Info
Comments
1 DFTPP 1 W1100155 RXI-2A-EA DFTPP
2 DFTPP 1 W1100156 RXI-2A-EA DFTPP
3 DailyCal 2 W1100157 RXI-2A-EA SSTD050 M8270 gcms22pg149
4 Blank 3 W1100158 RXI-2A-EA 0111-20 LCS
5 Sample 4 W1100159 RXI-2A-EA 0111-20 LCSD
6 Spike 4 W1100160 RXI-2A-EA 0111-20 LCSD
7 Spike 5 W1100161 RXI-2A-EA 0111-20 LCS (Conf)
8 Spike 6 W1100162 RXI-2A-EA 0111-20 LCSD (Conf)
9 Blank 7 W1100163 RXI-2A-EA CH2CI2 System Blank
10 Blank 7 W1100164 RXI-2A-EA CH2CI2 System Blank
11 Sample 8 W1100165 RXI-2A-EA 0111-20 MB
12 Sample 8 W1100166 RXI-2A-EA 0111-20 MB
13 Sample 9 W1100167 RXI-2A-EA 0111-20 ACB-M0010-FB
14 Sample 10 W1100168 RXI-2A-EA 0211-13 AE-15 SVGA PUF/XAD
15 Sample 11 W1100169 RXI-2A-EA 0211-13 AE-15 SVGA PUF/XAD *2
16 Sample 12 W1100170 RXI-2A-EA 0111-20 1R-ACB-M0010-02 *10
17 Sample 14 W1100171 RXI-2A-EA 0111-20 1R-ACB-M0010-02
18 Sample 15 W1100172 RXI-2A-EA 0111-20 1R-ACB-M0010-02 LD
19 Sample 13 W1100173 RXI-2A-EA 0111-20 1R-ACB-M0010-02 *10 LD
20
Inj @ 8:38am 2/21
gcmsprep2pg30 exp 3/15/11
Rep rep
EA# 0111-20 Page 118 of 126
-------
INSTRUMENT CONTROL PARAMETERS:
Wiley
M:\MS2010\METHODS\WILEY-EA\RXI-2A-EA.M
Thu Jun 10 12:52:48 2010
Control Information
Sample Inlet : GC
Injection Source : GC ALS
Mass Spectrometer : Enabled
6890 GC METHOD
OVEN
Initial temp: 35 'C (On)
Initial time: 0.50 min
Ramps:
# Rate Final temp Final time
1 25.00 310 0.00
2 4.00 330 2.00
3 O.O(Off)
Post temp: 0 'C
Post time: 0.00 min
Run time: 18.50 min
Maximum temp: 450
Equilibration time:
0.20 min
FRONT INLET (SPLIT/SPLITLESS)
Mode: Pulsed Splitless
Initial temp: 250 'C (On)
Pressure: 7.91 psi (On)
Pulse pressure: 30.0 psi
Pulse time: 0.40 min
Purge flow:
Purge time:
Total flow:
Gas saver:
Saver flow:
Saver time:
Gas type:
50.0 mL/min
0.30 min
54.0 mL/min
On
20.0 mL/min
1.00 min
Helium
BACK INLET (SPLIT/SPLITLESS)
Mode: Split
Initial temp: 50 'C (Off)
Pressure: 11.25 psi (On)
Split ratio: 50:1
Split flow: 43.6 mL/min
Total flow:
Gas saver:
Saver flow:
Saver time:
mL/min
51.!
On
20.0 mL/min
2.00 min
Gas type: Hydrogen
COLUMN 1
Capillary Column
Model Number: Restek 13638
Rxi-5Sil MS, 30m x 0.25mm x 0.Sum
Max temperature: 350 'C
Nominal length: 30.0 m
Nominal diameter: 250.00 urn
Nominal film thickness: 0.50 urn
Mode: constant flow
Initial flow: 1.1 mL/min
Nominal init pressure: 7.92 psi
Average velocity: 38 cm/sec
Inlet: Front Inlet
Outlet: MSB
Outlet pressure: vacuum
COLUMN 2
Capillary Column
Model Number: Restek 40111
Rtx-1 20m x 0.18mm x 0.4 urn
Max temperature: 340 'C
Nominal length: 20.0 m
Nominal diameter: 180.00 urn
Nominal film thickness: 0.40 urn
Mode: constant flow
Initial flow: 0.9 mL/min
Nominal init pressure: 11.25 psi
Average velocity: 42 cm/sec
Inlet: Back Inlet
Outlet: Back Detector
Outlet pressure: ambient
FRONT DETECTOR (NO DET)
BACK DETECTOR (FID)
Temperature: 250 'C (On)
Hydrogen flow: 40.0 mL/min (Off)
Air flow: 450.0 mL/min (Off)
Mode: Constant makeup flow
Makeup flow: 45.0 mL/min (Off)
Makeup Gas Type: Nitrogen
Flame: Off
Electrometer: On
Lit offset: 2.0
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
EA# 0111-20 Page 119 of 126
Page: 2
-------
SIGNAL 1
Data rate: 50 Hz
Type: mux'ed adc offset
Save Data: Off
Zero: 0.0 (Off)
Range: 0
Fast Peaks: Off
Attenuation: 0
SIGNAL 2
Data rate: 50 Hz
Type: mux'ed adc offset
Save Data: Off
Zero: 0.0 (Off)
Range: 0
Fast Peaks: Off
Attenuation: 0
COLUMN COMP 1
Derive from back detector
COLUMN COMP 2
Derive from back detector
THERMAL AUX 2
Use: MSD Transfer Line Heater
Description:
Initial temp: 280 'C (On)
Initial time: 0.00 min
# Rate Final temp Final time
1 O.O(Off)
POST RUN
Post Time: 0.00 min
TIME TABLE
Time
Specifier
Parameter & Setpoint
GC Injector
Front Injector:
Sample Washes
Sample Pumps
Injection Volume
Syringe Size
Prelnj Solvent A Washes
Prelnj Solvent B Washes
Postlnj Solvent A Washes
Postlnj Solvent B Washes
Viscosity Delay
Plunger Speed
Prelnjection Dwell
Postlnjection Dwell
Sampling Depth
Back Injector:
No parameters specified
1
3
0.20 microliters
10.0 microliters
0
0
5
5
2 seconds
Fast
0.00 minutes
0.00 minutes
2 . 0 mm
Column 1 Inventory Number
Column 2 Inventory Number
General Information
13638
40111
MS ACQUISITION PARAMETERS
Tune File
Acquistion Mode
dftpp060710d.u
Scan
MS Information
Solvent Delay
EMV Mode
Gain Factor
Resulting EM Voltage
2.90 min
Gain Factor
1.00
1588
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
EA# 0111-20 Page 120 of 126
Page: 3
-------
[Scan Parameters]
Low Mass
High Mass
Threshold
Sample #
[MSZones]
MS Source
MS Quad
35.0
500 . 0
100
2
A/D Samples
230 C maximum 250 C
150 C maximum 200 C
END OF MS ACQUISITION PARAMETERS
TUNE PARAMETERS for SN: US21844806
Trace Ion Detection is OFF.
EMISSION
ENERGY
REPELLER
IONFOCUS
ENTRANCE LE
EMVOLTS
AMUGAIN
AMUOFFSET
FILAMENT
DCPOLARITY
ENTLENSOFFS
14.306@502
MASSGAIN
MASSOFFSET
34.610
69.922
34.814
90.157
0.000
1541.176
2060.000
132.000
1.000
0.000
12.800®
14.306@799
69 . 000
-10.000
Actual EMV
GAIN FACTOR
12.800® 50
1588.23
1 . 02
16.314® 69
16.816@131
20.831@219
14.306@414
END OF TUNE PARAMETERS
END OF INSTRUMENT CONTROL PARAMETERS
RXI-2A-EA.M Thu Jun 10 12:52:10 2010
EA# 0111-20 Page 121 of 126
Page: 4
-------
This Is The Last Page
Of This Report.
EA# 0111-20 Page 122 of 126
-------
This Is The Last Page
Of This Report.
EA# 0111-20 Page 123 of 126
-------
This Is The Last Page
Of This Report.
EA# 0111-20 Page 124 of 126
-------
This Is The Last Page
Of This Report.
EA# 0111-20 Page 125 of 126
-------
This Is The Last Page
Of This Report.
EA# 0111-20 Page 126 of 126
-------
riangle Jr ar
311 I riaiigl
7201 ACCBlvd., Suite 104
Raleigh, NC 27617
919510-0228 Telephone
919510-0141 Fax
Jk
inc.
Web Site: www.rtp-labs.com
February 4, 2011
ARCADIS
4915 Prospectus Drive, Suite F
Durham, NC 27713
Attn: Brent Hall
PROJECT: "ACB Outlet Duct"; Project Number: RN990271.0002; PO: RB68419
RTF Labs ID: 11-096
Enclosed with this letter is the report on the chemical analysis for the Tedlar bag sample received on
January 21, 2011 for a normal turnaround. The sample was analyzed by EPA Method TO-15/0040
GC/MS for 60 VOC target compounds and a library search for unknown VOCs (Tentatively Identified
Compounds).
Please call if you have any questions.
Sincerely,
Alston Sykes, Principal Chemist
Attachments: GC/MS report, COC form
File: Arcadis 11-096
Page 1 of 5
-------
Je
7201 ACCBlvd., Suite 104
Raleigh, NC 27617
sn IT
0,1
Jk
inc.
919510-0228 Telephone
919510-0141 Fax
Web Site: www.rtp-labs.com
EPA Method TO-15 GC/MS VOLATILE ORGANICS
Data File: c:\varianws\wsdatafiles\voc113010\11-096-01b.sms Acquisition Date: 1/21/2011
Comment: Arcadis/ EPA IR-ACB-M0040-01 1/21/11; 10 ml; DF=5
CAS NO.
75-71 -8
76-14-2
74-87-3
75-01 -4
106-99-0
74-83-9
75-00-3
75-69-4
75-35-4
76-13-1
64-17-5
75-15-0
67-63-0
75-09-2
67-64-1
156-60-5
1 1 -05-3
1634-04-4
75-34-3
108-05-4
156-59-2
1 1 0-82-7
67-66-3
141-78-6
109-99-9
71 -55-6
56-23-5
78-93-3
142-82-5
71 -43-2
107-06-2
79-01 -6
78-87-5
75-27-4
123-91-1
10061-01-5
108-88-3
108-10-1
1006-02-6
127-18-4
79-00-5
124-48-1
106-93-4
591-78-6
100-41-4
108-90-7
1330-20-7
95-47-6
100-42-5
75-25-2
79-34-5
622-96-8
108-67-8
95-63-6
541-73-1
106-46-7
100-44-7
95-50-1
87-68-3
120-82-1
COMPOUND
Dichlorodifluoromethane (Freon 12)
1 ,2-Chloro-1 ,1 ,2,2-Tetrafluoroethane
Chloromethane
Vinyl chloride
1,3-Butadiene
Bromomethane
Chloroethane
Trichloromonofluoromethane
1,1-dichloroethene
1 ,1 ,2-trichloro-1 ,2,2-trifluoroethane
Ethanol
Carbon disulfide
Isopropyl alcohol
Methylene chloride
Acetone
t-1 ,2-dichloroethene
Hexane
Methyl-t-butyl ether (MTBE)
1,1-Dichloroethane
Vinyl acetate
cis-1 ,2-dichloroethene
Cyclohexane
Chloroform
Ethyl Acetate
Tetrahydrofuran
1,1,1-trichloroethane
Carbon Tetrachloride
2-Butanone
Heptane
Benzene
1,2-dichloroethane
Trichloroethylene
1,2-dichloropropane
Bromodichloromethane
1 ,4-dioxane
cis-1 ,3-dichloropropene
Toluene
4-Methyl-2-pentanone (MIBK)
t-1 ,3-dichloropropene
Tetrachloroethylene
1,1,2-trichloroethane
Dibromochloromethane
1 ,2-dibromoethane
2-Hexanone
Ethylbenzene
Chlorobenzene
m/p-Xylene
o-Xylene
Styrene
Tribromomethane
1 ,1 ,2,2-tetrachloroethane
1 -ethyl-4-methylbenzene
1 ,3,5-trimethylbenzene
1 ,2,4-trimethylbenzene
1 ,3-dichlorobenzene
1 ,4-dichlorobenzene
Benzyl chloride
1,2-dichlorobenzene
1 ,1 ,2,3,4,4-hexachloro-1 ,3-butadiene
1 ,2,4-trichlorobenzene
CONCENTRATION
Not Found
Not Found
10.03
Not Found
121.07
Not Found
1.73
Not Found
Not Found
Not Found
1.71
1.70
3.59
Not Found
1 04.40
Not Found
12.62
Not Found
Not Found
Below MDL
Not Found
Below MDL
Not Found
4.22
1.43
Not Found
Not Found
Not Found
1.81
323.74
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
42.66
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
1.78
5.43
Not Found
3.42
1.36
5.92
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
Not Found
UNITS MDL and Reporting Limit
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
ppbv 1
File: Arcadis 11-096
Page 2 of 5
-------
1 HP • 1
nil JL nangle
7201 ACCBlvd., Suite 104
Raleigh, NC 27617
919510-0228 Telephone
919510-0141 Fax
sn IT
0,1
Jk
inc.
Web Site: www.rtp-labs.com
TENTATIVELY IDENTIFIED COMPOUNDS
EPA Method TO-15
Data File: c:\varianws\wsdatafiles\vod 13010\11 -096-01 b.sms
Comment: Arcadis/ EPA IR-ACB-M0040-01 1/21/11; 10 ml; DF=5
CAS NO.
151-56-4
151-56-4
186205-18-
54060-80-9
21618-99-5
460-19-5
146724-75-
115-07-1
115-11-7
460-19-5
2516-34-9
10575-41-4
2562-38-1
7051 -34-5
513-35-9
110-00-9
460-19-5
185-94-4
106-90-1
3638-35-5
75-05-8
763-30-4
460-19-5
534-22-5
4723-10-8
109862-24-
4025-37-0
28290-01-9
110-02-1
97-72-3
25350-22-5
625-74-1
625-74-1
(IS)isBFB
1/24/2011
COMPOUND NAME
GC/MS VOLATILE ORGANICS
Acquisition Date: 1/21/2011
Retention Time Estimated Concentration,
Ethylenimine
Ethylenimine
Di(1,2,5-oxadiazolo)[3,4-b;3,4-E]pyrazin
N-(2-Methylacryloyl)imidazola
5-(2-Aminopropyl)-2-methylphenol
Ethanedinitrile
2,5-Dimethoxy-4-(methylsulfonyl)amphetam
Propene
1-Propene, 2-methyl-
Ethanedinitrile
Cyclobutylamine
Hex-4-yn-3-one
Cyclopentane, nitro-
Cyclopropane, (bromomethyl)-
2-Butene, 2-methyl-
Furan
Ethanedinitrile
Bicyclo[2.1.0]pentane
2-Propenoic acid, oxiranylmethyl ester
Propane, 2-cyclopropyl-
Acetonitrile
1,4-Pentadiene, 2-methyl-
Ethanedinitrile
Furan, 2-methyl-
1,5-Hexadiene-3,4-diol, 2,5-dimethyl-
Propane, 1-acetoxy-2-isocyano-
(2-Aziridinylethyl)amine
Cyclobutanone, 2,3,3-trimethyl-
Thiophene
Propanoic acid, 2-methyl-, anhydride
Pentanamide, 2-(dimethylamino)-3-methyl-
Propane, 2-methyl-1-nitro-
Propane, 2-methyl-1-nitro-
Internal Standard and (SS) are Surrogate Standards that are
11:57 Page 1 of 2
8.15
8.18
8.29
8.38
8.54
8.58
8.66
8.70
9.32
9.41
9.72
10.2
10.3
10.6
11.0
11.3
12.0
12.2
12.3
13.4
14.1
14.3
14.7
15.0
16.2
16.6
16.9
17.1
17.6
17.7
18.2
18.3
18.5
added to each sample.
CLP TIC
5.21
8.77
0.87
15.23
2.13
2.15
1.91
0.77
0.97
6.61
4.19
1.11
3.71
0.83
0.41
3.97
1.22
2.23
8.01
7.02
10.11
1.09
29.90
1.15
1.11
1.77
4.33
3.91
0.61
0.54
14.74
0.61
0.26
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
11-096-01 b.sms
File: Arcadis 11-096
Page 3 of 5
-------
Je
7201 ACCBlvd., Suite 104
Raleigh, NC 27617
919510-0228 Telephone
919510-0141 Fax
sn IT
0,1
Jk
inc.
Web Site: www.rtp-labs.com
CAS NO.
2978-58-7
14617-88-0
4786-20-3
821-55-6
36566-80-0
872-56-0
None
286-76-0
16411-13-5
3683-19-0
625-74-1
81 4-78-8
498-51-1
10374-74-0
536-74-3
None
542-54-1
52805-36-4
620-1 4-4
127-19-5
33868-50-7
673-32-5
108-95-2
7531 4-1 9-1
16484-17-6
4930-03-4
2981 2-79-1
121-19-7
91 -20-3
COMPOUND NAME
3-Butyn-2-amine, 2-methyl-
2-Butenoic acid, 4-nitrophenyl ester, (E
2-Butenenitrile
2-Nonanone
3-Hexyne, 2-methyl-
Isopropylcyclobutane
4-Methyl-2,4-bis(4'-trimethylsilyloxyphe
Bicyclo[7.1 .0]decane
Butanedinitrile, 2,3-dimethyl-
(Z)-4-Methyl-2-hexene
Propane, 2-methyl-1-nitro-
3-Buten-2-one, 3-methyl-
2-Heptanone, 6-methyl-5-methylene-
7-Tetradecene
Phenylethyne
Ethyl [5-hydroxy-1 -(6-methoxy-4-methyl-3
Pentanenitrile, 4-methyl-
4-Benzyloxybenzonitrile
Benzene, 1-ethyl-3-methyl-
Acetamide, N,N-dimethyl-
Ethanone, 2-(benzoyloxy)-1 -phenyl-
Benzene, 1-propynyl-
Phenol
Cyclobutanone, 2-methyl-2-oxiranyl-
2H-lnden-2-one, octahydro-, trans-
Carbamic acid, phenyl-, phenyl ester
Hydroxylamine, O-decyl-
Roxarsone
Naphthalene
Retention Time Estimated Concentration,
18.6
18.9
19.1
19.6
19.7
20.1
20.6
21.2
21.3
21.5
21.5
21.6
22.0
22.3
23.1
23.6
23.8
24.1
24.2
24.7
25.4
25.9
26.1
26.5
26.6
26.9
27.7
27.9
28.1
1.60
3.64
1.11
0.95
0.47
4.87
0.71
0.54
1.94
0.69
0.13
1.32
0.59
3.08
5.28
4.77
2.04
0.57
0.77
41.42
13.04
2.19
43.28
2.06
0.99
0.47
0.86
0.77
5.36
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
ppbv
(IS) is BFB Internal Standard and (SS) are Surrogate Standards that are added to each sample.
1/24/2011 11:57 Page 2 of 2 CLP TIC
11-096-01 b.sms
File: Arcadis 11-096
Page 4 of 5
-------
Je
7201 ACCBlvd., Suite 104
Raleigh, NC 27617
919510-0228 Telephone
919510-0141 Fax
sn IT
0,1
Jk
inc.
Web Site: www.rtp-labs.com
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
US EPA
RTP, NC
ACB Outlet Duct
RN99027 1.0002
RTP Labs
Sample ID
1R-ACB-M0040-01
Sample Matrix
Tedlar bag
Notes/Comments: |
Analysis Requested
VOCs
_ /
Relinquished By:
Date/Time:
Received By:
Date/Time:
%-^m' JJ&rd
J/2/j/y/ -=- SZ30
f~F 4\ J^l-,, ,
'/^•z/-^// /^7ao
Relinquished By:
Date/Time:
Received By:
Date/Time:
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919)544-4535
Fax Number: (919)544-5690
File: Arcadis 11-096
Page 5 of 5
-------
ARCADIS-US: Durham
4915 Prospectus Drive, Suite F
Durham, NC 27713
ACB Outlet Duct
Client RN990271.0002
PO # RB68535
Analytical Report
(0211-49)
EPA Method TO-15
TO-15 Target Compound List
Enthalpy Analytical, Inc.
) 850-4392 / Fax:(919)850-9012 / www.e
2202 Ellis Road Durham, NC 27703 - 5518
|yt» ft Phone:(919)850-4392 / Fax:(919)850-9012 / www.enthalpy.com
-------
I certify that to the best of my knowledge all analytical data presented in this report:
• Have been checked for completeness
• Are accurate, error-free, and legible
• Have been conducted in accordance with approved protocol, and that all deviations and
analytical problems are summarized in the appropriate narrative(s)
This analytical report was prepared in Portable Document Format (.PDF) and contains 106 pages.
QA Review Performed by: Michael Steven Schapira
Report Issued: 3/2/11
EA# 0211-49 Page 2 of 106
-------
Results
EA# 0211-49 Page 3 of 106
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method TO-1 5
1 Run & 1 FB
Client #
Job*
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/201 1
Sample ID: 0211-49 1 R-ACB-M0040-02 *5,000
Data File: L11 00448. D
Tank/Misc ID: 10mL load of 100* Syr dil
Compound
ropylene
reon 12 (CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
,3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
crolein
reon113(C2CI3F3)
,1-Dichloroethene
cetone
arbon disulfide
iopropyl alcohol
llyl chloride (3-chloropropene)
cetonitrile
lethylene chloride
ans-1 ,2-Dichloroethene
lethyl tert-butyl
crylonitrile
exane
ether
,1-Dichloroethane
inyl acetate
s-1 ,2-Dichloroethylene
lethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
,1 ,1-Trichloroethane
yclohexane
arbon tetrachloride
enzene
,2,4-trimethylpentane
,2-Dichloroethane
eptane
richloroethene
,2-Dichloropropane
lethyl methacrylate
,4-Dioxane
romodichloromethane
s-1 ,3-Dichloropropene
lethyl isobutyl ketone
oluene
ans-1 ,3-Dichloropropene
,1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
ibromochloromethane
Quant
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
Ret.
Time
(min)
4.14
NA
NA
NA
NA
5.11
NA
NA
NA
NA
NA
7.90
NA
NA
8.22
NA
NA
NA
9.02
9.14
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
12.42
NA
NA
NA
13.51
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
16.65
NA
NA
NA
NA
NA
Area
8,589
0
0
0
0
37,224
0
0
0
0
0
1,964
0
0
2,962,902
0
0
0
138,299
16,188
0
0
0
0
0
0
0
0
0
0
15,560
0
0
0
35,829
0
0
0
0
0
0
0
0
0
0
35,300
0
0
0
0
0
Cone, as
Analyzed
(ppbv)
0.567
0.200
0.200
0.200
0.200
1.33
0.200
0.200
0.200
0.200
0.200
0.270
0.200
0.200
57.6
0.200
0.200
0.200
8.36
0.462
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
1.57
0.200
0.200
0.200
0.505
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.310
0.200
0.200
0.200
0.200
0.200
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
2,835
1,000
1,000
1,000
1,000
6,631
1,000
1,000
1,000
1,000
1,000
1,352
1,000
1,000
288,146
1,000
1,000
1,000
41,820
2,311
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
7,832
1,000
1,000
1,000
2,524
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,000
1,551
1,000
1,000
1,000
1,000
1,000
5,000
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
J
ND
ND
E
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
J
ND
ND
ND
ND
ND
2/22/2011
-------
Company ARCADIS-US:
Analyst TDD
Durham
Parameters EPA Method TO-15
# Samples 1 Run & 1 FB
Client #RN990271. 0002
Job #021 1-49
PO # RB68535
Report Date 2/22/201 1
Sample ID: 0211-49 1 R-ACB-M0040-02 *5,000
Data File: L11 00448.
Tank/MiscID: lOmLload
Compound
,2-Dibromoethane
hlorobenzene
thylbenzene
,1,1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
, 1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
,3,5-Trimethylbenzene
,2,4-Trimethylbenzene
,3-Dichlorobenzene
,4-Dichlorobenzene
enzyl chloride
,2-Dichlorobenzene
,2,4-Trichloro benzene
exachlorobutadiene
aphthalene
romochloromethane (IS)
,4-Difluorobenzene (IS)
hlorobenzene-d5 (IS)
D
oMOO'Syrdil
Ret.
Quant Time
(min)
107 NA
112 NA
91 NA
131 NA
91 NA
91 NA
104 NA
173 NA
83 NA
105 NA
91 NA
105 NA
105 NA
146 NA
146 NA
91 NA
146 NA
180 NA
225 NA
128 NA
130 12.42
114 14.22
117 18.63
Cone, as
Area Analyzed
(ppbv)
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
0 0.200
954,897 11.24
2,810,714 11.93
2,090,202 11.13
DF: 5,000
TankDF: 1.00
ConCal 1
Sample
Cone. Flags
(ppbv)
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
1,000 ND
Rec. (%)
N/A
N/A
N/A
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.l
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.42
14.22
18.63
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
954,897
2,810,714
2,090,202
Flag
Pass
Pass
Pass
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method TO-15
1 Run & 1 FB
Client #
Job#
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/2011
Sample ID: 0211-49 1R-ACB-M0040-02 *10,000
Data File: L1100449.D
Tank/Misc ID: 5mL load of 100* Syr dil
DF: 10,000
TankDF: 1.00
ConCal 1
Acetone
Compound
Quant
43
Ret.
Time
(min)
8.20
Area
1,600,353
Cone, as
Analyzed
(ppbv)
28.6
Sample
Cone.
(ppbv)
286,045
Flags
Rec. (%)
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
130
114
117
12.43
14.22
18.63
1,039,112
3,002,647
1,662,386
11.24
11.93
11.13
N/A
N/A
N/A
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.)
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.43
14.22
18.63
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
1,039,112
3,002,647
1,662,386
Flag
Pass
Pass
Pass
2/22/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-1 5
# Samples 1 Run & 1 FB
Client #
Job*
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/2011
Sample ID: 0211-49 ACB-M0040-FB *50
Data File: L11 00445. D
Tank/MiscID: lOmLload
Compound
ropylene
reon12(CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
,3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
crolein
reon113(C2CI3F3)
,1-Dichloroethene
cetone
arbon disulfide
iopropyl alcohol
llyl chloride (3-chloropropene)
cetonitrile
lethylene chloride
ans-1 ,2-Dichloroethene
lethyl tert-butyl ether
crylonitrile
exane
,1-Dichloroethane
inyl acetate
s-1 ,2-Dichloroethylene
lethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
,1,1-Trichloroethane
yclohexane
arbon tetrachloride
enzene
,2,4-trimethylpentane
,2-Dichloroethane
eptane
richloroethene
,2-Dichloropropane
lethyl methacrylate
,4-Dioxane
romodichloromethane
s-1 ,3-Dichloropropene
lethyl isobutyl ketone
oluene
ans-1 ,3-Dichloropropene
, 1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
ibromochloromethane
Ret.
Quant Time
(min)
41 NA
85 NA
85 NA
50 NA
62 NA
39 NA
94 NA
64 NA
106 NA
101 NA
45 NA
56 NA
151 NA
61 NA
43 8.22
76 NA
45 NA
76 NA
41 9.02
49 9.14
61 NA
73 NA
53 NA
57 NA
63 NA
43 NA
61 NA
72 NA
45 NA
83 NA
72 NA
97 NA
56 NA
117 NA
78 NA
57 NA
62 NA
57 NA
130 NA
63 NA
69 NA
88 NA
83 NA
75 NA
43 NA
91 NA
75 NA
97 NA
166 NA
43 NA
129 NA
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
210,422
0
0
0
104,678
8,150
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
3.94
0.200
0.200
0.200
6.10
0.224
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
197
10.0
10.0
10.0
305
11.2
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
50.0
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
J
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
3/2/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-1 5
# Samples 1 Run & 1 FB
Client #
Job*
PO#
Report Date
RN990271.
0211-49
RB68535
2/22/2011
Sample ID: 0211-49 ACB-M0040-FB *50
Data File: L11 00445. D
Tank/MiscID: lOmLload
Compound
,2-Dibromoethane
hlorobenzene
thylbenzene
,1,1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
,1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
,3, 5-Trimethyl benzene
,2,4-Trimethylbenzene
,3-Dichloro benzene
,4-Dichloro benzene
enzyl chloride
,2-Dichloro benzene
,2,4-Trichloro benzene
exachlorobutadiene
aphthalene
romochloromethane (IS)
,4-Difluorobenzene (IS)
hlorobenzene-d5 (IS)
Quant
107
112
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
130
114
117
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
12.42
14.21
18.62
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
990,822
2,931,311
2,156,026
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
11.24
11.93
11.13
0002
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
10.0
Rec. (%)
N/A
N/A
N/A
50.0
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.)
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.42
14.21
18.62
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
990,822
2,931,311
2,156,026
Flag
Pass
Pass
Pass
3/2/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-15
# Samples 1 Run & 1 FB
Sample ID: humid blank
Data File: L11 00439. D
Tank/Misc ID: Can #1470, 500mL
Compound
ropylene
reon 12 (CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
,3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
crolein
reon113(C2CI3F3)
,1-Dichloroethene
cetone
arbon disulfide
iopropyl alcohol
llyl chloride (3-chloropropene)
cetonitrile
lethylene chloride
ans-1 ,2-Dichloroethene
lethyl tert-butyl ether
crylonitrile
exane
,1-Dichloroethane
inyl acetate
s-1 ,2-Dichloroethylene
lethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
,1 ,1-Trichloroethane
yclohexane
arbon tetrachloride
enzene
,2,4-trimethylpentane
,2-Dichloroethane
eptane
richloroethene
,2-Dichloropropane
lethyl methacrylate
,4-Dioxane
romodichloromethane
s-1 ,3-Dichloropropene
lethyl isobutyl ketone
oluene
ans-1 ,3-Dichloropropene
,1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
ibromochloromethane
Quant
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
7.43
NA
NA
NA
8.23
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
Client #
Job*
PO#
Report Date
Area
0
0
0
0
0
0
0
0
0
0
2,306
0
0
0
24,346
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
RN990271.0002
0211-49
RB68535
2/22/201 1
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.224
0.200
0.200
0.200
0.455
0.200
0.200
0.200
0.930
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.224
0.200
0.200
0.200
0.455
0.200
0.200
0.200
0.930
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
1.00
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
J
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
2/22/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-15
# Samples 1 Run & 1 FB
Sample ID: humid blank
Data File: L11 00439. D
Tank/Misc ID: Can #1470, 500mL
Compound
,2-Dibromoethane
hlorobenzene
thylbenzene
,1,1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
, 1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
,3,5-Trimethylbenzene
,2,4-Trimethylbenzene
,3-Dichlorobenzene
,4-Dichlorobenzene
enzyl chloride
,2-Dichlorobenzene
,2,4-Trichloro benzene
exachlorobutadiene
aphthalene
romochloromethane (IS)
,4-Difluorobenzene (IS)
hlorobenzene-d5 (IS)
Quant
107
112
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
130
114
117
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
12.41
14.21
18.60
Client #
Job*
PO#
Report Date
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
993,298
2,977,691
2,158,507
RN990271.0002
0211-49
RB68535
2/22/201 1
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
11.24
11.93
11.13
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
Rec. (%)
N/A
N/A
N/A
1.00
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.l
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.41
14.21
18.60
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
993,298
2,977,691
2,158,507
Flag
Pass
Pass
Pass
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method TO-1 5
1 Run & 1 FB
Client #
Job*
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/201 1
Sample ID: system blank
Data File: L11 00440. D
Tank/Misc ID: 500mL
Compound
ropylene
reon 12 (CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
,3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
crolein
reon113(C2CI3F3)
,1-Dichloroethene
cetone
arbon disulfide
iopropyl alcohol
llyl chloride (3-chloropropene)
cetonitrile
lethylene chloride
ans-1 ,2-Dichloroethene
lethyl tert-butyl
crylonitrile
exane
ether
,1-Dichloroethane
inyl acetate
s-1 ,2-Dichloroethylene
lethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
,1 ,1-Trichloroethane
yclohexane
arbon tetrachloride
enzene
,2,4-trimethylpentane
,2-Dichloroethane
eptane
richloroethene
,2-Dichloropropane
lethyl methacrylate
,4-Dioxane
romodichloromethane
s-1 ,3-Dichloropropene
lethyl isobutyl ketone
oluene
ans-1 ,3-Dichloropropene
,1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
ibromochloromethane
Ret.
Quant Time
(min)
41 NA
85 NA
85 NA
50 NA
62 NA
39 NA
94 NA
64 NA
106 NA
101 NA
45 NA
56 NA
151 NA
61 NA
43 8.24
76 NA
45 NA
76 NA
41 NA
49 NA
61 NA
73 NA
53 NA
57 NA
63 NA
43 NA
61 NA
72 NA
45 NA
83 NA
72 NA
97 NA
56 NA
117 NA
78 NA
57 NA
62 NA
57 NA
130 NA
63 NA
69 NA
88 NA
83 NA
75 NA
43 NA
91 NA
75 NA
97 NA
166 NA
43 NA
129 NA
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
23,834
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.519
0.200
0.200
0.200
0.930
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.519
0.200
0.200
0.200
0.930
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
1.00
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
2/22/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-15
# Samples 1 Run & 1 FB
Sample ID: system blank
Data File: L11 00440. D
Tank/Misc ID: 500mL
Compound
,2-Dibromoethane
hlorobenzene
thylbenzene
,1,1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
, 1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
,3,5-Trimethylbenzene
,2,4-Trimethylbenzene
,3-Dichlorobenzene
,4-Dichlorobenzene
enzyl chloride
,2-Dichlorobenzene
,2,4-Trichloro benzene
exachlorobutadiene
aphthalene
romochloromethane (IS)
,4-Difluorobenzene (IS)
hlorobenzene-d5 (IS)
Quant
107
112
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
130
114
117
Ret.
Time
(min)
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
12.41
14.20
18.60
Client #
Job*
PO#
Report Date
Area
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
853,741
2,484,956
1,881,446
RN990271.0002
0211-49
RB68535
2/22/201 1
Cone, as
Analyzed
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
11.24
11.93
11.13
DF:
Tank DF:
ConCal 1
Sample
Cone.
(ppbv)
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
0.200
Rec. (%)
N/A
N/A
N/A
1.00
1.00
Flags
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.l
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.41
14.20
18.60
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
853,741
2,484,956
1,881,446
Flag
Pass
Pass
Pass
2/22/2011
-------
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method TO-1 5
1 Run & 1 FB
Client #
Job*
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/2011
Sample ID: Sppbv LCS std A
Data File: L11 00437. D
Tank/MiscID: Can #1434 "A", 125mL
Com pound
ropylene
reon 12 (CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
crolein
reon113(C2CI3F3)
1-Dichloroethene
cetone
arbon disulfide
iopropyl alcohol
llyl chloride (3-chloropropene)
cetonitrile
ethylene chloride
ans-1 ,2-Dichloroethene
ethyl tert-butyl ether
crylonitrile
exane
1-Dichloroethane
inyl acetate
s-1 ,2-Dichloroethylene
ethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
1 , 1-Trichloroethane
yclohexane
arbon tetrachloride
enzene
2,4-trimethylpentane
2-Dichloroethane
eptane
richloroethene
2-Dichloropropane
ethyl methacrylate
4-Dioxane
romodichloromethane
s-1 ,3-Dichloropropene
ethyl isobutyl ketone
oluene
ans-1 ,3-Dichloropropene
1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
ibromochloromethane
Quant
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
Ret.
Time
(min)
4.14
4.23
4.56
4.74
5.02
5.10
5.88
6.14
6.56
6.66
7.40
7.87
7.82
7.89
8.22
8.33
8.58
8.79
9.02
9.13
9.60
9.56
9.84
9.99
10.62
10.70
11.90
11.98
11.98
12.54
12.42
12.80
12.75
13.03
13.49
13.41
13.68
13.76
14.58
15.01
15.12
15.19
15.43
16.15
16.37
16.62
17.16
17.44
17.50
17.72
17.94
Area
Cone
DF: 1.00
TankDF: 1.00
ConCal 1
. as Sample
Analyzed
(ppbv)
84,996
781,732
697,153
114,387
153,572
138,396
406,642
73,459
428,632
1,005,355
53,647
38,903
502,719
254,753
295,479
429,730
264,146
57,230
93,403
181,630
217,768
429,867
67,630
151,306
240,127
399,838
202,090
58,433
44,200
509,420
56,800
681,089
155,414
810,106
397,347
487,392
298,769
96,159
445,685
124,035
122,319
149,576
584,728
276,490
382,575
690,229
261,931
333,888
579,472
320,899
961,155
5.45
5.04
5.00
5.03
4.77
4.78
4.81
4.65
5.26
5.32
5.27
5.20
5.62
5.32
5.58
5.33
5.69
5.35
5.48
5.03
5.36
5.38
5.34
5.47
5.23
5.43
5.38
5.80
5.11
5.46
5.55
5.44
5.22
5.57
5.42
5.21
5.55
5.43
5.52
5.31
5.50
5.50
5.45
5.75
5.57
5.48
4.98
5.33
5.44
5.40
5.70
Cone. Flags
(ppbv)
5.45
5.04
5.00
5.03
4.77
4.78
4.81
4.65
5.26
5.32
5.27
5.20
5.62
5.32
5.58
5.33
5.69
5.35
5.48
5.03
5.36
5.38
5.34
5.47
5.23
5.43
5.38
5.80
5.11
5.46
5.55
5.44
5.22
5.57
5.42
5.21
5.55
5.43
5.52
5.31
5.50
5.50
5.45
5.75
5.57
5.48
4.98
5.33
5.44
5.40
5.70
Tag Value
5.50
4.90
4.90
4.95
4.90
5.15
4.90
4.90
5.10
5.05
5.35
5.15
5.15
5.15
5.30
5.20
5.50
5.30
4.65
5.20
5.15
5.20
5.05
5.30
5.10
5.25
5.15
5.30
5.20
5.20
5.35
5.10
5.15
5.10
5.10
4.90
5.10
5.20
5.15
5.15
5.05
5.15
5.15
5.30
5.25
5.20
4.85
5.20
5.10
5.40
5.40
%r\itt
uin.
From Tag
1.0
2.8
1.9
1.5
2.6
7.1
1.9
5.1
3.1
5.3
1.5
0.9
9.2
3.4
5.2
2.5
3.4
1.0
17.9
3.2
4.1
3.5
5.8
3.2
2.6
3.4
4.4
9.3
1.7
5.0
3.7
6.7
1.3
9.2
6.2
6.2
8.8
4.4
7.2
3.1
8.9
6.8
5.9
8.5
6.1
5.4
2.6
2.6
6.6
0.0
5.6
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
2/22/2011
-------
Company ARCADIS-US: Durham
Analyst TDD
Parameters EPA Method TO-15
# Samples 1 Run & 1 FB
Sample ID: Sppbv LCS std A
Data File: L11 00437. D
Client #
Job*
PO#
Report Date
RN990271.0002
0211-49
RB68535
2/22/2011
Tank/MiscID: Can #1434 "A", 125mL
Com pound
2-Dibromoethane
hlorobenzene
thylbenzene
1 , 1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
3,5-Trimethylbenzene
2,4-Trimethylbenzene
3-Dichlorobenzene
4-Dichlorobenzene
enzyl chloride
2-Dichlorobenzene
2,4-Trichloro benzene
exachlorobutadiene
aphthalene
romochloromethane (IS)
4-Difluorobenzene (IS)
hlorobenzene-d5 (IS)
Quant
107
112
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
130
114
117
Ret.
Time
(min)
18.11
18.64
18.70
18.73
18.83
19.25
19.28
19.53
19.95
20.07
20.10
20.12
20.45
20.74
20.82
20.93
21.13
22.45
22.50
22.71
12.41
14.21
18.61
Area
696,177
832,916
990,575
534,955
1,595,242
829,103
725,152
909,734
593,847
1,421,831
1,034,550
1,164,626
1,173,734
872,832
849,408
814,865
864,405
371,405
553,005
1,010,521
984,010
2,904,255
2,312,408
Cone
.as
Analyzed
(ppbv)
5.12
5.35
5.24
5.50
10.3
5.39
5.23
5.45
5.33
5.23
4.87
5.10
5.26
5.56
5.33
5.46
5.38
4.44
4.82
4.37
11.24
11.93
11.13
DF: 1.00
TankDF: 1.00
ConCal 1
Sample
Cone. Flags
(ppbv)
5.12
5.35
5.24
5.50
10.3
5.39
5.23
5.45
5.33
5.23
4.87
5.10
5.26
5.56
5.33
5.46
5.38
4.44
4.82
4.37
Rec. (%)
N/A
N/A
N/A
Tag Value
5.10
5.25
5.20
5.20
10.20
5.30
5.20
5.10
5.30
5.20
4.85
5.15
5.20
5.25
5.10
5.25
5.15
5.10
5.15
5.25
%niff
LJIII.
From Tag
0.5
1.9
0.8
5.7
0.8
1.8
0.5
6.9
0.5
0.7
0.4
0.9
1.2
5.8
4.5
4.0
4.4
13.0
6.4
16.9
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.l
Internal Standard
Name
Bromochloromethane (IS)
1, 4-Difluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.41
14.20
18.60
Sample
RT
12.41
14.21
18.61
Ave. ICal
IS Area
1,089,684
3,198,975
2,556,498
Sample
IS Area
984,010
2,904,255
2,312,408
Flag
Pass
Pass
Pass
2/22/2011
-------
Narrative Summary
^ 3
1 *lj
^"" EA# 0211 -49 Page 15 of 106
-------
Enthalpy Analytical Narrative Summary
Company
Analyst
Parameters
# Samples
ARCADIS-US: Durham
TDD
EPA Method TO- 15
1 Run & 1 FB (Tedlar Bags)
Client #
Job#
PO#
Report Date
RN99027 1.0002
0211-49
RB68535
February 22, 20 11
Custody Heather Tarjeft received the Tedlar bag samples on 2/17/11 after being
relinquished by ARCADIS-US: Durham. The samples were received at
ambient temperature and in good condition. Prior to, during, and after
analysis, the sample was kept under lock with access only to authorized
personnel by Enthalpy Analytical, Inc.
Analysis The tedlar bag samples were analyzed for TO-15 Target Compound List
using the analytical procedures in EPA Method TO-15, Determination of
Volatile Organic Compounds (VOCs) In Air Collected In Specially-
Prepared Canisters And Analyzed by Gas Chromatography/Mass
Spectrometry (GC/MS). The samples were analyzed following the
procedures in Section 10.0, Sample Analysis.
The field blank was analyzed at a 50-fold GC/MS analytical dilution.
Sample 1R-ACB-M0040-02 was analyzed at a 5,000-fold GC/MS
analytical dilution to meet a client requested MLD of 1 ppmv. This
sample was also analyzed at a 10,000-fold GC/MS analytical dilution to
bring acetone within the calibration range. Only acetone is reported in the
higher dilution-level analyses. The E-flagged result value for this
compound should be disregarded in favor of the result in the more dilute
analysis. The dilution factor is recorded on the Quantitation Report in the
Sample field. A five thousand-fold dilution is indicated by: df=*5,000.
The Hewlett Packard Model 5890, Gas Chromatograph ("Lurch" S/N
2750A15233) was equipped with a 5971A Mass Selective Detector and a
Restek Rtx-624 60 m x 0.32 mm x 1.8 urn (S/N 890248) capillary column.
All samples and standards were introduced directly to the analyzer using
an Entech 7100 A Preconcentrator.
Calibration The calibration curve is included in the Calibration Curve Chromatograms
section of this report.
Chromatographic A copy of the acquisition method (MM624-L.M) is included in the
Conditions Calibration Curve Chromatograms section of this report.
QC Notes All target compounds met the 30% initial calibration RSD criteria
specified in the method.
" «
"I «» t ml*" I
" ',*
EA# 0211 -49 Page 16 of 106
-------
Enthalpy Analytical Narrative Summary
(continued)
QC Notes
(continued)
Reporting Notes
The ICV recovery values were within 30% of the expected value for all
target compounds.
The analysis of the LCS met the QC limits of within 30% of the expected
value for all compounds.
All internal standard responses met QC criteria.
Ethanol and acetone were identified in the humid blank analyzed with
these samples, at 0.224 ppbv and 0.455 ppbv respectively. The system
blank also included an acetone result of 0.519 ppbv. These concentrations
fall within the method's acceptable range for blank contamination of three
times the method detection limit (3 x 0.2 ppbv = 0.6 ppbv).
The lab duplicate associated with this data met QC criteria of less than
25% difference of the original analysis for reported compounds.
The sample was analyzed within the 30 day holding time required by the
method.
Acetonitrile is a known laboratory contaminant, therefore the MDL for
this compound has been elevated to 0.930 ppbv.
Enthalpy Analytical, Inc. is accredited to perform this method for
compliance purposes by the National Environmental Laboratory
Accreditation Conference (NELAC) through the Louisiana Environmental
Laboratory Accreditation Program (LELAP), certificate number 04010.
The results presented in this report are representative of the samples as
provided to the laboratory.
> fJ" j>t ^ W
k-ft-Av*! *•' *
9, A F Fi ^», . ,
EA# 0211 -49 Page 17 of 106
-------
General Reporting Notes
The following are general reporting notes that are applicable to all Enthalpy Analytical, Inc. data
reports, unless specifically noted otherwise.
• The acronym MDL represents the Minimum Detection Limit. Below this value the laboratory cannot
determine the presence of the analyte of interest reliably.
• The acronym LOQ represents the Limit of Quantification. Below this value the laboratory cannot
quantitate the analyte of interest within the criteria of the method.
• The acronym ND following a value indicates a non-detect or analytical result below the MDL.
• The letter / following a value indicates an analytical result between the MDL and the LOQ. A J flag
indicates that the laboratory can positively identify the analyte of interest as present, but the value
should be considered an estimate.
• The letter E following a value indicates an analytical result exceeding 100% of the highest calibration
point. The associated value should be considered as an estimate.
• The acronym DF represents Dilution Factor. This number represents dilution of the sample during the
preparation and/or analysis process. The analytical result taken from a laboratory instrument is
multiplied by the DF to determine the final undiluted sample results.
• The addition of MS to the Sample ID represents a Matrix Spike. An aliquot of an actual sample is
spiked with a known amount of analyte so that a percent recovery value can be determined. This shows
what effect the sample matrix may have on the target analyte, i.e. whether or not anything in the sample
matrix interferes with the analysis of the analyte(s).
• The addition of MSD to the Sample ID represents a Matrix Spike Duplicate. Prepared in the same
manner as an MS, the use of duplicate matrix spikes allows further confirmation of laboratory quality
by showing the consistency of results gained by performing the same steps multiple times.
• The addition of LD to the Sample ID represents a Laboratory Duplicate. The analyst prepares an
additional aliquot of sample for testing and the results of the duplicate analysis are compared to the
initial result. The result should have a difference value of within 10% of the initial result (if the results
of the original analysis are greater than the LOQ).
• The addition of AD to the Sample ID represents an Alternate Dilution. The analyst prepares an
additional aliquot at a different dilution factor (usually double the initial factor). This analysis helps
confirm that no additional compound is present and coeluting or sharing absorbance with the analyte of
interest, as they would have a different response/absorbance than the analyte of interest.
• The Sample ID LCS represents a Laboratory Control Sample. Clean matrix, similar to the client sample
matrix, prepared and analyzed by the laboratory using the same reagents, spiking standards and
procedures used for the client samples. The LCS is used to assess the control of the laboratory's
analytical system. Whenever spikes are prepared for our client projects, two extra spikes are prepared.
The extras (randomly chosen) are labeled with the associated project number and kept in-house at the
appropriate temperature conditions. When the project samples are received for analysis, the LCSs are
analyzed to confirm that the analyte could be recovered from the media, separate from the samples
which were used on the project and which may have been affected by source matrix, sample collection
and/or sample transport.
EA# 0211 -49 Page 18 of 106
-------
General Reporting Notes
(continued)
Significant Figures: Where the reported value is much greater than unity (1.00) in the units expressed,
the number is rounded to a whole number of units, rather than to 3 significant figures. For example, a
value of 10,456.45 ug catch is rounded to 10,456 ug. There are five significant digits displayed, but no
confidence should be placed on more than two significant digits.
Manual Integration: The data systems used for processing will flag manually integrated peaks with
an "M". There are several reasons a peak may be manually integrated. These reasons will be identified
by the following two letter designations. The peak was not integrated by the software "NI", the peak
was integrated incorrectly by the software "II" or the wrong peak was integrated by the software
"WP". These codes will accompany the analyst's manual integration stamp placed next to the
compound name.
EA# 0211 -49 Page 19 of 106
-------
Method Detection Limits for EPA Method TO-14 and EPA Method TO-15
Enthalpy performs an annual Method Detection Limit (MDL) study to determine detection limits
for each of the target analyte compounds for TO-14/TO-15. At least 7 injections are made of the
low level standard (1 ppbv). The concentrations are pulled into an Excel spreadsheet and the
standard deviation value is determined. This value is then multiplied by the appropriate Student's
T value (based on the number of replicates analyzed) to determine the MDL value. Any
calculated values below 0.200 ppbv are disregarded (and replaced with 0.200) because they are
less than a fifth of the low standard. In 2007 nine injections of the low standard were analyzed
and all but six (exceptions are shaded in the table below) of the target analytes had MDL values
below 0.200, and so are reported using 0.200 as the MDL on the table below and in all reports.
Any analyte which was not identified in a sample shows the MDL as the concentration as
analyzed. Compounds present in samples have the measured concentration displayed. To
determine the detection limit for a compound which was present in a sample multiply the MDL
from the table below by any tank or analytical dilution factor.
Compound
MDL
(ppbv)
Propylene 0.256
Freon 12 (CC12F2) 0.202
Freonll4(C2C12F4) 0.200
Chloromethane 0.200
Chloroethene (Vinyl chloride) 0.200
1,3-Butadiene 0.200
Bromomethane 0.200
Chloroethane 0.229
Bromoethene (Vinyl bromide) 0.200
Freon 11 (CC13F) 0.200
Freon 113 (C2C13F3) 0.200
1,1-Dichloroethene 0.200
Acetone 0.200
Carbon disulfide 0.200
Isopropyl alcohol 0.200
Allyl chloride (3-chloropropene) 0.200
Methylene chloride 0.200
trans- 1,2-Dichloroethene 0.200
Methyl tert-butyl ether 0.200
Hexane 0.200
1,1-Dichloroethane 0.200
Vinyl acetate 0.200
cis-l,2-Dichloroethylene 0.200
Methyl ethyl ketone (2-Butanone) 0.200
Ethyl acetate 0.200
Chloroform 0.200
Tetrahydrofuran 0.200
1,1,1-Trichloroethane 0.200
Cyclohexane 0.200
Carbon tetrachloride 0.200
Benzene 0.200
2,2,4-trimethylpentane 0.200
Compound
MDL
(ppbv)
1,2-Dichloroethane 0.200
Heptane 0.200
Trichloroethene 0.200
1,2-Dichloropropane 0.200
1,4-Dioxane 0.200
Bromodichloromethane 0.200
cis-1,3-Dichloropropene 0.200
Methyl isobutyl ketone 0.200
Toluene 0.200
trans-1,3-Dichloropropene 0.200
1,1,2-Trichloroethane 0.200
Tetrachloroethene 0.200
2-Hexanone (Methyl butyl ketone) 0.201
Dibromochloromethane 0.200
1,2-Dibromoethane 0.200
Chlorobenzene 0.200
Ethylbenzene 0.200
m-/p-Xylenes 0.200
o-Xylene 0.200
Styrene 0.200
Bromoform 0.200
1,1,2,2-Tetrachloroethane 0.200
4-Ethyltoluene 0.200
1,3,5-Trimethylbenzene 0.200
1,2,4-Trimethylbenzene 0.200
1,3-Dichlorobenzene 0.200
1,4-Dichlorobenzene 0.200
Benzyl chloride 0.200
1,2-Dichlorobenzene 0.200
1,2,4-Trichlorobenzene 0.227
Hexachlorobutadiene 0.268
TO-14 /TO-15 MDL Table for 2007-2008
EA# 0211-49 Page 20 of 106
-------
Sample Custody
EA# 0211 -49 Page 21 of 106
-------
CHAIN OF CUSTODY RECORD
US EPA
RTF. NC
ACB Outlet Duct
RN990271.0002
Enthalpy
Sample ID
1R-ACB-M0040-02
ACB-M0040-FB
Sample Matrix
Tedlar bag
Tedkr bag filled with UHP nitrogen
Analysis Requested
VOCs
VOCs
/^.& J^£^g_
L^2£SE
Notes/Comments:
1
Field blank collected 2/9/11. Emission sample collected 2/16/11.
ARCADIS U.S., Inc.
49 15 Prospectus Drive, Suite F
Durham, North Carolina 27713
*'V|V\|\\
Phone Number: (919)544-4535
Fax Number: (919)544-5690
EA# 021 1-49 Page 22 of 106
-------
Sample
Chromatograms
EA# 0211-49 Page 23 of 106
-------
Data File
Acq On
Sample
Misc
Quantitation Report
C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100448.D Vial: 1
18 Feb 2011 1:50 pm Operator: tdd/cld
: 0211-49 1R-ACB-M0040-02 *5,000
: lOmL load of 100* Syr dil
MS Integration Params: rteint.p
Quant Time: Feb 18 15:06 2011
Inst
Lurch
Multiplr: 1.00
Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
TIC: L1100448. D
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
0
L110044
if
I
o
if
I
3.
if
ra
1 I
1
c m
II ' I
i
1
|
Ul
I
s
L n *
4.00 5.00 6.00 7.00 8.00 9.00 10.00 1iQpJ2J»J 3.00 M.QQ 15.00 J^DO 17,00. 13.00 19.0020.0021.0022.0023.0024.0025.0026.00
8.D L021111A.M Fri Feb 18 15:06:53 2011 Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100448.D Vial: 1
Acq On : 18 Feb 2011 1:50 pm Operator: tdd/cld
Sample : 0211-49 1R-ACB-M0040-02 *5,000 Inst : Lurch
Misc : lOmL load of 100* Syr dil Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 15:06 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.42
.22
.63
130
114
117
954897
2810714
2090202
11.
11.
11.
24
93
13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
0
0
0
0
5
0
0
0
0
0
7
0
0
8
0
0
0
9
9
0
0
0
0
0
0
0
0
0
0
12
0
0
0
13
0
0
0
0
0
0
0
0
0
0
16
0
0
0
0
0
0
0
.14
.00
.00
.00
.00
.11
.00
.00
.00
.00
.00
.90
.00
.00
.22
.00
.00
.00
.02
.14
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.42
.00
.00
.00
.51
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.65
.00
.00
.00
.00
.00
.00
.00
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
8589
0
0
0
0
37224
0
0
0
0
0
1964
0
0
2962902
0
0
0
138299
16188
0
0
0
0
0
0
0
0
0
0
15560
0
0
0
35829
0
0
0
0
0
0
0
0
0
0
35300
0
0
0
0
0
0
0
0.
N
N
N
N
1.
N
N
N
N
N
0.
N
N
57.
N
N
N
8.
0.
N
N
N
N
N
N
N
N
N
N
1.
N
N
N
0.
N
N
N
N
N
N
N
N
N
N
0.
N
N
N
N
N
N
N
57
.D
.D
.D
.D
33
.D
.D
.D
.D
.D
27
.D
.D
63
.D
.D
.D
36
46
.D
.D
.D
.D
.D
.D
.D
.D
.D
.D
57
.D
.D
.D
50
.D
.D
.D
.D
.D
.D
.D
.D
.D
.D
31
.D
.D
.D
.D
.D
.D
.D
PPBV
.
.
. d
. d
PPBV
. d
.
.
. d
.
PPBV
.
.
PPBV
. d
. d
.
PPBV
PPBV
.
.
.
. d
.
.
.
.
.
.
PPBV
.
.
.
PPBV
.
.
.
.
.
.
.
.
.
.
PPBV
.
.
.
.
.
.
•
0.02
0.02
0.04
Qvalue
# 1
91
# 10
# 92
# 84
94
89
95
98
(#) = qualifier out of range (m) =
L1100448.D L021111A.M Fri Feb 18 15:06:51 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100448.D Vial: 1
Acq On : 18 Feb 2011 1:50 pm Operator: tdd/cld
Sample : 0211-49 1R-ACB-M0040-02 *5,000 Inst : Lurch
Misc : lOmL load of 100* Syr dil Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 15:06 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 0.00 91
58) 1,1,1,2-Tetrachloroethane 0.00 131
59) m-/p-Xylenes 0.00 91
60) o-Xylene 0.00 91
61) Styrene 0.00 104
62) Bromoform 0.00 173
63) 1,1,2,2-Tetrachloroethane 0.00 83
64) 4-Ethyltoluene 0.00 105
65) 2-Chlorotoluene 0.00 91
66) 1,3,5-Trimethylbenzene 0.00 105
67) 1,2,4-Trimethylbenzene 0.00 105
68) 1,3-Dichlorobenzene 0.00 146
69) 1,4-Dichlorobenzene 0.00 146
70) Benzyl chloride 0.00 91
71) 1,2-Dichlorobenzene 0.00 146
72) 1,2,4-Trichlorobenzene 0.00 180
73) Hexachlorobutadiene 0.00 225
74) Naphthalene 0.00 128
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
L1100448.D L021111A.M
Fri Feb 18 15:06:52 2011
Page 2
-------
Ref50
Scan 94 (4.123 min): L102751.D (-)
40 60 80 100 120 140 160 180 200
Raw50
40 60 80 100 120 140 160 180 200
Sub
50
Ref50
0
- r-p-i ii | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i T
40 60 80 100 120 140 160 180 200
Scan 188 (5.068 min): L102751. D
20 40 60 80 100 120 140 160 180 200 220 240 260 280
Raw50
20 40 60 80 100 120 140 160 180 200 220 240 260 280
Sub
50
0" I I l~l"T"T~l"TTrTTTTTTTTTTrm I I TTT I I I I I I IT
20 40 60 80 100120140160180200220240260280
#2
Propylene
Concen: 0.57 PPBV
RT: 4.14 min Scan# 110
Delta R.T. 0.01 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 41 Resp: 8589
Ion Ratio Lower Upper
41 100
39 110.6 0.0 0.0#
42 60.2 1.7 2.5#
Ion 41.10 (40.80 to 41.80): L1
6000
4000
2000
0
4.05 4.10 4.15 4.20 4.25
#7
1,3-Butadiene
Concen: 1.33 PPBV
RT: 5.11 min Scan# 206
Delta R.T. 0.02 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 39 Resp: 37224
Ion Ratio Lower Upper
39 100
53 51.8 37.7 56.5
54 68.4 48.5 72.7
20000
15000
10000
5000
Ion 39.00 (38.70 to 39.70): L1
5.00 5.10 5.20
L1100448.D L021111A.M
EA# 0211-49 Page 27 of 106
Fri Feb 18 15:07:14 2011
Page 3
-------
Ref50
Scan 455 (7.753 min): L102751.D (-)
#13
Acrolein
Concen: 0.27 PPBV
RT: 7.90 min Scan# 483
Delta R.T. 0.03 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
20 40 60 80 100 120 140 160 180 200 220 240 260 T9t Ion: 56 ResP: 1964
Ion Ratio Lower Upper
56 100
55 0.0 62.6 93.8#
Raw50
0
Ion 55.95 (55.65 to 56.65): L1
20 40 60 80 100 120 140 160 180 200 220 240 260
Sub
50
20 40 60 80 100 120 140 160 180 200 220 240 260
Scan 492 (8.126 min): L102751. D
800
600
400
200
0
Ref50
7.80 7.85 7.90 7.95
#16
Acetone
Concen: 57.63 PPBV
RT: 8.22 min Scan# 515
Delta R.T. -0.00 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
20
Raw50
40 60 80 100120140160180200220240260 T9t Ion: 43 ResP: 2962902
Ion Ratio Lower Upper
43 100
58 16.7 10.7 16.1#
Ion 43.00 (42.70 to 43.70): L1
1000000
20
Sub
40 60 80 100 120 140 160 180 200 220 240 260 800000
600000
400000
200000
0
~20 40 60 80 100 120 140 160 180 200 220 240 260
50
8.10 8.20 8.30 8.40
L1100448.D L021111A.M
EA# 0211-49 Page 28 of 106
Fri Feb 18 15:07:14 2011
Page 4
-------
Ref50
Scan 567 (8.880 min): L102751. D
20 40 60 80 100 120 140 160 180 200 220 240 260 280
Raw50
0
20 40 60 80 100 120 140 160 180 200 220 240 260 280
Sub
50
0
20 40 60 80 100 120 140 160 180 200 220 240 260 280
Scan 580 (9.011 min): L102751. D
Ref50
40 60 80 100 120 140 160 180 200
Raw50
"T"1
40 60 80 100 120 140 160 180 200
Sub
50
40 60 80 100 120 140 160 180 200
#20
Acetonitrile
Concen: 8.36 PPBV
RT: 9.02 min Scan# 595
Delta R.T. 0.02 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 41 Resp:
Ion Ratio Lower
41 100
40
39
38
60000
61.9
25.6
19.5
55.4
30.6
25.9
138299
Upper
83.2
45. 8#
38.9#
Ion 40.95 (40.65 to 41.65): L1
Ion 37.90 (37.60 to 38.60): L1
40000
20000
8.90 9.00 9.10 9.20
#21
Methylene chloride
Concen: 0.46 PPBV
RT: 9.14 min Scan# 607
Delta R.T. 0.02 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 49 Resp: 16188
Ion Ratio Lower Upper
49 100
84 93.5 70.2 105.4
6000
4000
2000
Ion 49.05 (48.75 to 49.75): L1
9.10 9.20 9.30
L1100448.D L021111A.M
EA# 0211-49 Page 29 of 106
Fri Feb 18 15:07:14 2011
Page 5
-------
Ref50
Scan 904 (12.269 min): L102751.D (-)
20 40 60 80 100 120 140 160 180 200
Raw50
0
20 40 60 80 100 120 140 160 180 200
Sub
50
0
20 40 60 80 100 120 140 160 180 200
Scan 1010 (13.336 min): L102751.D
Ref50
Raw50
Sub
50
40 60 80 100 120 140 160 180 200
#32
Tetrahydrofuran
Concen: 1.57 PPBV
RT: 12.42 min Scan# 933
Delta R.T. 0.02 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 72 Resp: 15560
Ion Ratio Lower Upper
72 100
71 97.8 75.9 113.9
42 250.6 181.4 272.2
Ion 72.00 (71.70 to 72.70): L1
10000
5000
0
12130 12^40 12^50
#37
Benzene
Concen: 0.50 PPBV
RT: 13.51 min Scan# 1041
Delta R.T. 0.03 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 78 Resp: 35829
Ion Ratio Lower Upper
78 100
77 24.4 19.9 29.9
50 20.6 20.2 30.4
Ion 78.10 (77.80 to 78.80): L1
40 60 80 100 120 140 160 180 200
15000
10000
5000
40 60 80 100 120 140 160 180 200
13.40 13.50 13.60
L1100448.D L021111A.M
EA# 0211-49 Page 30 of 106
Fri Feb 18 15:07:15 2011
Page 6
-------
Ref50
Raw50
Sub
50
Scan 1321 (16.464 min): L102751.D
20 40 60 80 100 120 140 160 180 200
0
20 40 60 80 100 120 140 160 180 200
0
20 40 60 80 100 120 140 160 180 200
#49
Toluene
Concen: 0.31 PPBV
RT: 16.65 min Scan# 1353
Delta R.T. 0.03 min
Lab File: L1100448.D
Acq: 18 Feb 2011 1:50 pm
Tgt Ion: 91 Resp: 35300
Ion Ratio Lower Upper
91 100
92 60.0 49.1 73.7
Ion 91.10 (90.80 to 91.80): L1
10000
5000
0
16.50 16.60 16.70 16.80
L1100448.D L021111A.M
EA# 0211 -49 Page 31 of 106
Fri Feb 18 15:07:15 2011
Page 7
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100449.D Vial: 1
Acq On : 18 Feb 2011 2:24 pm Operator: tdd/cld
Sample : 0211-49 1R-ACB-M0040-02 *10,000 Inst : Lurch
Misc : 5mL load of 100* Syr dil Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 15:08 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
TIC: L1100449. D
2600000
2500000
2400000
2300000
2200000
2100000
2000000
1900000
1800000
1700000
1600000
1500000
1400000
1300000
1200000
1100000
1000000
900000
800000
700000
600000
500000
400000
300000
200000
100000
n
— ^
en g.
1
1
55"
I
J
1
1
°
^jL^^-v^
.c
1
L^^j
v___
I
i i
! 6
L „ ^_
L_L
L________~_ , „ . „ *—,
4.00 5.00 6.00 7.00 8.00 9.00 10.001.
L1100449.D L021111A.M Fri Feb 18 15:08:50 2011
i.OO 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100449.D Vial: 1
Acq On : 18 Feb 2011 2:24 pm Operator: tdd/cld
Sample : 0211-49 1R-ACB-M0040-02 *10,000 Inst : Lurch
Misc : 5mL load of 100* Syr dil Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 15:08 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.43
.22
.63
130
114
117
1039112
3002647
1662386
11.
11.
11.
24 PPBV
93 PPBV
13 PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2, 4-trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1,2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
0
0
0
0
0
5
0
0
0
0
0
0
0
0
8
0
0
0
9
9
0
0
0
0
0
0
0
0
0
0
12
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.00
.00
.00
.00
.00
.12
.00
.00
.00
.00
.00
.00
.00
.00
.20
.00
.00
.00
.04
.15
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.44
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
0
0
0
0
0
17051
0
0
0
0
0
0
0
0
1600353
0
0
0
42660m
8054
0
0
0
0
0
0
0
0
0
0
5479
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N
N
N
N
N
0.
N
N
N
N
N
N
N
N
28.
N
N
N
2.
0.
N
N
N
N
N
N
N
N
N
N
0.
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
.D. d
.D.
.D.
.D.
.D. d
56 PPBV
.D.
.D.
.D.
.D. d
.D.
.D.
.D.
.D.
60 PPBV
.D. d
.D. d
.D.
37 PPBV
21 PPBV
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
51 PPBV
.D.
.D.
.D.
.D. d
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D. d
.D.
.D.
.D.
.D.
.D.
.D.
.D.
0.02
0.01
0.03
Qvalue
# 88
# 92
99
# 72
(#) = qualifier out of range (m) =
L1100449.D L021111A.M Fri Feb 18 15:08:49 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100449.D Vial: 1
Acq On : 18 Feb 2011 2:24 pm Operator: tdd/cld
Sample : 0211-49 1R-ACB-M0040-02 *10,000 Inst : Lurch
Misc : 5mL load of 100* Syr dil Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 15:08 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 0.00 91
58) 1,1,1,2-Tetrachloroethane 0.00 131
59) m-/p-Xylenes 0.00 91
60) o-Xylene 0.00 91
61) Styrene 0.00 104
62) Bromoform 0.00 173
63) 1,1,2,2-Tetrachloroethane 0.00 83
64) 4-Ethyltoluene 0.00 105
65) 2-Chlorotoluene 0.00 91
66) 1,3,5-Trimethylbenzene 0.00 105
67) 1,2,4-Trimethylbenzene 0.00 105
68) 1,3-Dichlorobenzene 0.00 146
69) 1,4-Dichlorobenzene 0.00 146
70) Benzyl chloride 0.00 91
71) 1,2-Dichlorobenzene 0.00 146
72) 1,2,4-Trichlorobenzene 0.00 180
73) Hexachlorobutadiene 0.00 225
74) Naphthalene 0.00 128
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
L1100449.D L021111A.M
Fri Feb 18 15:08:49 2011
Page 2
-------
CD
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O
P
cn
o
o
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100445.D Vial: 1
Acq On : 18 Feb 2011 12:02 pm Operator: tdd/cld
Sample : 0211-49 ACB-M0040-FB *50 Inst : Lurch
Misc : lOmL load Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 13:12 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
TIC: L1100445. D
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
4.00 5.00 6.00 7.00 8.00 9.00 10.001.
L1100445.D L021111A.M Fri Feb 18 13:13:06 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100445.D Vial: 1
Acq On : 18 Feb 2011 12:02 pm Operator: tdd/cld
Sample : 0211-49 ACB-M0040-FB *50 Inst : Lurch
Misc : lOmL load Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 13:12 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.42
.21
.62
130
114
117
990822
2931311
2156026
11.
11.
11.
24 PPBV
93 PPBV
13 PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
0
0
0
0
0
0
0
0
0
0
0
0
0
0
8
0
0
0
9
9
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.22
.00
.00
.00
.02
.14
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
0
0
0
0
0
0
0
0
0
0
0
0
0
0
210422
0
0
0
104678
8150
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N
N
N
N
N
N
N
N
N
N
N
N
N
N
3.
N
N
N
6.
0.
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
.D.
.D.
.D.
.D.
.D. d
.D. d
.D. d
.D. d
.D.
.D. d
.D.
.D.
.D.
.D.
94 PPBV
.D. d
.D. d
.D.
10 PPBV
22 PPBV
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D. d
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D.
.D. d
.D.
.D.
.D. d
.D.
.D. d
.D.
.D.
0.01
0.00
0.02
Qvalue
# 90
# 81
98
(#) = qualifier out of range (m) =
L1100445.D L021111A.M Fri Feb 18 13:13:05 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100445.D Vial: 1
Acq On : 18 Feb 2011 12:02 pm Operator: tdd/cld
Sample : 0211-49 ACB-M0040-FB *50 Inst : Lurch
Misc : lOmL load Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 13:12 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 0.00 91
58) 1,1,1,2-Tetrachloroethane 0.00 131
59) m-/p-Xylenes 0.00 91
60) o-Xylene 0.00 91
61) Styrene 0.00 104
62) Bromoform 0.00 173
63) 1,1,2,2-Tetrachloroethane 0.00 83
64) 4-Ethyltoluene 0.00 105
65) 2-Chlorotoluene 0.00 91
66) 1,3,5-Trimethylbenzene 0.00 105
67) 1,2,4-Trimethylbenzene 0.00 105
68) 1,3-Dichlorobenzene 0.00 146
69) 1,4-Dichlorobenzene 0.00 146
70) Benzyl chloride 0.00 91
71) 1,2-Dichlorobenzene 0.00 146
72) 1,2,4-Trichlorobenzene 0.00 180
73) Hexachlorobutadiene 0.00 225
74) Naphthalene 0.00 128
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
L1100445.D L021111A.M
Fri Feb 18 13:13:06 2011
Page 2
-------
Ref50
Scan 492 (8.126 min): L102751. D
20 40 60 80 100 120 140 160 180 200
Raw50
#16
Acetone
Concen: 3.94 PPBV
RT: 8.22 min Scan# 516
Delta R.T. 0.00 min
Lab File: L1100445.D
Acq: 18 Feb 2011 12:02 pm
Tgt Ion: 43 Resp: 210422
Ion Ratio Lower Upper
43 100
58 17.5 10.7 16.1#
Ion 43.00 (42.70 to 43.70): L1
0
20 40 60 80 100 120 140 160 180 200
Sub
50
60000
40000
20000
20
"T1"
40
60 80 100 120 140 160 180 200
8.10 8.20 8.30 8.40
Ref50
Raw50
Sub
50
Scan 567 (8.880 min): L1 02751. D #20
Acetonitrile
Concen : 6
.10 PPBV
RT: 9.02 min Scan# 595
Delta R.T.
I Lab File:
Acq: 18 Feb
i
40 60 80100120140160180200220240260280 T9t Ion: 41
lon Ratio
41 100
40 57.2
39 26.1
38 18.4
lon 40.95
40000
TTTTJ r~n i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i i | i i i
40 60 80 100 120 140 160 180 200 220 240 260 280 -----
oUUUU
20000
10000
,- 0
0.01 min
L1100445.D
2011 12:02 pm
Resp: 104678
Lower Upper
55.4 83.2
30.6 45. 8#
25.9 38. 9#
(40.65 to 41. 65): L1
lon 37.90 (37.60 to 38.60): L1
I
/
//
A
A
I
\
\
r\ \
~^^:-~
40 60 80 100 120 140 160 180 200 220 240 260 280
8.90 9.00 9.10 9.20
L1100445.D L021111A.M
EA# 0211-49 Page 39 of 106
Fri Feb 18 13:14:01 2011
Page 3
-------
Ref50
Raw50
Sub
50
Scan 580 (9.011 min): L102751. D
40 60 80 100 120 140 160 180 200
#21
Methylene chloride
Concen: 0.22 PPBV
RT: 9.14 min Scan# 607
Delta R.T. 0.01 min
Lab File: L1100445.D
Acq: 18 Feb 2011 12:02 pm
Tgt Ion: 49 Resp: 8150
Ion Ratio Lower Upper
49 100
84 89.7 70.2 105.4
40 60 80 100 120 140 160 180 200
3000
2000
1000
Ion 49.05 (48.75 to 49.75): L1
40 60 80 100 120 140 160 180 200
9.10 9.20
L1100445.D L021111A.M
EA# 0211-49 Page 40 of 106
Fri Feb 18 13:14:01 2011
Page 4
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100439.D Vial: 1
Acq On : 17 Feb 2011 7:28 pm Operator: tdd/cld
Sample : humid blank Inst : Lurch
Misc : Can #1470, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 12:47 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
TIC: L1100439. D
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
n
a?
|
|
S
o
^
CO
I
s
to
I
s
1 1 1
4.00 5.00 6.00 7.00 8.00 9.00 10.001,
L1100439.D L021111A.M Fri Feb 18 13:10:54 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100439.D Vial: 1
Acq On : 17 Feb 2011 7:28 pm Operator: tdd/cld
Sample : humid blank Inst : Lurch
Misc : Can #1470, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 12:47 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.41
.21
.60
130
114
117
993298
2977691
2158507
11.24 PPBV
11.93 PPBV
11.13 PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
0
0
0
0
0
0
0
0
0
0
7
0
0
0
8
0
0
0
9
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.43
.00
.00
.00
.23
.00
.00
.00
.13
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
0
0
0
0
0
0
0
0
0
0
2306
0
0
0
24346
0
0
0
5812
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D. d
N.D.
N.D.
N.D. d
0.22 PPBV
N.D.
N.D.
N.D.
0.46 PPBV
N.D. d
N.D. d
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
0.00
0.00
0.00
Qvalue
# 1
# 89
#T\
(#) = qualifier out of range (m) =
L1100439.D L021111A.M Fri Feb 18 13:10:53 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100439.D Vial: 1
Acq On : 17 Feb 2011 7:28 pm Operator: tdd/cld
Sample : humid blank Inst : Lurch
Misc : Can #1470, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 12:47 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 0.00 91
58) 1,1,1,2-Tetrachloroethane 0.00 131
59) m-/p-Xylenes 0.00 91
60) o-Xylene 0.00 91
61) Styrene 0.00 104
62) Bromoform 0.00 173
63) 1,1,2,2-Tetrachloroethane 0.00 83
64) 4-Ethyltoluene 0.00 105
65) 2-Chlorotoluene 0.00 91
66) 1,3,5-Trimethylbenzene 0.00 105
67) 1,2,4-Trimethylbenzene 0.00 105
68) 1,3-Dichlorobenzene 0.00 146
69) 1,4-Dichlorobenzene 0.00 146
70) Benzyl chloride 0.00 91
71) 1,2-Dichlorobenzene 0.00 146
72) 1,2,4-Trichlorobenzene 0.00 180
73) Hexachlorobutadiene 0.00 225
74) Naphthalene 0.00 128
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
L1100439.D L021111A.M
Fri Feb 18 13:10:53 2011
Page 2
-------
CD
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o
o
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100440.D Vial: 1
Acq On : 17 Feb 2011 8:15 pm Operator: tdd/cld
Sample : system blank Inst : Lurch
Misc : SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 7:17 2011
Quant Results File: L021111A.RES
Method
Title
Last Update
Response via
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100440. D
4.00 5.00 6.00 7.00 8.00 9.00 10.00 liQDJZJWJ3.0014.0315.00
CA\TT \)£ I I ~^rix i 3Q6
L1100440.D L021111A.M Fri Feb 18 13:11:05 2011
i.OO 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100440.D Vial: 1
Acq On : 17 Feb 2011 8:15 pm Operator: tdd/cld
Sample : system blank Inst : Lurch
Misc : SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 7:17 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.41
.20
.60
130
114
117
853741
2484956
1881446
11.24 PPBV
11.93 PPBV
11.13 PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
0
0
0
0
0
0
0
0
0
0
0
0
0
0
8
0
0
0
y
9
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.24
.00
.00
.00
. u ^
.12
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
ft -L
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
0
0
0
0
0
0
0
0
0
0
0
0
0
0
23834
0
0
0
4814
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N.D.
N.D.
N.D.
N.D.
N.D.
N.D. d
N.D. d
N.D.
N.D.
N.D. d
N.D. d
N.D.
N.D.
N.D.
0.52 PPBV
N.D. d
N.D.
N.D.
\J . / A ±*±*J3 V
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
0.00
0.00
0.00
Qvalue
# 67
76
(#) = qualifier out of range (m) =
L1100440.D L021111A.M Fri Feb 18 13:11:04 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100440.D Vial: 1
Acq On : 17 Feb 2011 8:15 pm Operator: tdd/cld
Sample : system blank Inst : Lurch
Misc : SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 18 7:17 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 0.00 91
58) 1,1,1,2-Tetrachloroethane 0.00 131
59) m-/p-Xylenes 0.00 91
60) o-Xylene 0.00 91
61) Styrene 0.00 104
62) Bromoform 0.00 173
63) 1,1,2,2-Tetrachloroethane 0.00 83
64) 4-Ethyltoluene 0.00 105
65) 2-Chlorotoluene 0.00 91
66) 1,3,5-Trimethylbenzene 0.00 105
67) 1,2,4-Trimethylbenzene 0.00 105
68) 1,3-Dichlorobenzene 0.00 146
69) 1,4-Dichlorobenzene 0.00 146
70) Benzyl chloride 0.00 91
71) 1,2-Dichlorobenzene 0.00 146
72) 1,2,4-Trichlorobenzene 0.00 180
73) Hexachlorobutadiene 0.00 225
74) Naphthalene 0.00 128
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
N.D.
L1100440.D L021111A.M
Fri Feb 18 13:11:05 2011
Page 2
-------
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CQ
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100437.D Vial: 1
Acq On : 17 Feb 2011 5:55 pm Operator: tdd/cld
Sample : Sppbv LCS std A Inst : Lurch
Misc : Can #1434 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 19:00 2011
Method
Title
Last Update
Response via
4800000
4600000
4400000
4200000
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
0
Quant Results File: L021111A.RES
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100437. D
o
s
4.00 5.00 6.00 7.00 8.00 9.00 10.001,
L1100437.D L021111A.M Fri Feb 18 13:10:30 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100437.D Vial: 1
Acq On : 17 Feb 2011 5:55 pm Operator: tdd/cld
Sample : Sppbv LCS std A Inst : Lurch
Misc : Can #1434 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 19:00 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.41
.21
.61
130
114
117
984010
2904255
2312408
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1,2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.14
.23
.56
.74
.02
.10
.88
.14
.56
.66
.40
.87
.82
.89
.22
.33
.58
.79
.02
.13
.60
.56
.84
.99
.62
.70
.90
.98
.98
.54
.42
.80
.75
.03
.49
.41
.68
.76
.58
.01
.12
.19
.43
.15
.37
.62
.16
.44
.50
.72
.94
.11
.64
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
84996
781732
697153
114387
153572
138396
406642
73459
428632
1005355
53647
38903
502719
254753
295479
429730
264146
57230
93403
181630
217768
429867
67630
151306
240127
399838
202090
58433
44200
509420
56800
681089
155414
810106
397347
487392
298769
96159
445685
124035
122319
149576
584728
276490
382575
690229
261931
333888
579472
320899
961155
696177
832916
5
5
5
5
4
4
4
4
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
5
5
5
5
5
5
.45
.04
.00
.03
.77
.78
.81
.65
.26
.32
.27
.20
.62
.32
.58
.33
.69
.35
.48
.03
.36
.38
.34
.47
.23
.43
.38
.80
.11
.46
.55
.44
.22
.57
.42
.21
.55
.43
.52
.31
.50
.50
.45
.75
.57
.48
.98
.33
.44
.40
.70
.12
.35
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.01
Qvalue
# 1
98
# 94
97
98
88
99
97
# 98
99
# 61
98
# 100
# 74
# 91
93
# 100
99
# 88
89
# 79
100
93
90
99
95
# 74
82
95
99
97
95
95
99
95
91
100
92
98
98
90
94
99
99
99
99
# 91
92
96
# 94
100
98
94
(#) = qualifier out of range (m) =
L1100437.D L021111A.M Fri Feb 18 13:10:30 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100437.D Vial: 1
Acq On : 17 Feb 2011 5:55 pm Operator: tdd/cld
Sample : Sppbv LCS std A Inst : Lurch
Misc : Can #1434 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 19:00 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.70 91 990575
58) 1,1,1,2-Tetrachloroethane 18.73 131 534955
59) m-/p-Xylenes 18.83 91 1595242
60) o-Xylene 19.25 91 829103
61) Styrene 19.28 104 725152
62) Bromoform 19.53 173 909734
63) 1,1,2,2-Tetrachloroethane 19.95 83 593847
64) 4-Ethyltoluene 20.07 105 1421831
65) 2-Chlorotoluene 20.10 91 1034550
66) 1,3,5-Trimethylbenzene 20.12 105 1164626m
67) 1,2,4-Trimethylbenzene 20.45 105 1173734
68) 1,3-Dichlorobenzene 20.74 146 872832
69) 1,4-Dichlorobenzene 20.82 146 849408m
70) Benzyl chloride 20.93 91 814865
71) 1,2-Dichlorobenzene 21.13 146 864405
72) 1,2,4-Trichlorobenzene 22.45 180 371405
73) Hexachlorobutadiene 22.50 225 553005m
74) Naphthalene 22.71 128 1010521
5.24
5.50
10.28
5.39
5.23
5.45
5.33
5.23
.87
.10
5.26
5.56
5.33
5.46
5.38
4.44
4.82
4.37
4.
5.
PPBV
PPBV
PPBV
PPBV
PPBV #
PPBV #
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
95
91
91
91
91
99
99
100
[Manual Int. "WP"
99
Manual Int. "WP" (TDDJ
96
99
99
L1100437.D L021111A.M
Fri Feb 18 13:10:30 2011
Page 2
-------
Calibration
Curve Chromatograms
EA# 0211-49 Page 52 of 106
-------
Response Factor Report
Sample ID
0.4ppbvto15stdA
1ppbvto15stdA
2ppbv to15 std A
5ppbv to-15 std A
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
LID
0.4
1
2
5
10
20
30
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
Acq. Date
02/11/11 14:40
02/11/11 15:23
02/11/11 16:42
02/10/11 20:50
02/10/11 21:32
02/10/11 22:19
02/10/11 23:10
Inst: Lurch
Curve: L021111A.I
# Name
2) Propylene
3) Freon12(CCI2F2)
4) Freon114(C2CI2F4)
5) Chloromethane
6) Chloroethene (Vinyl chloride)
7) 1,3-Butadiene
8) Bromomethane
9) Chloroethane
10) Bromoethene (Vinyl bromide)
11) Freon11 (CCI3F)
12) Ethanol
13) Acrolein
14) Freon113(C2CI3F3)
15) 1,1-Dichloroethene
16) Acetone
17) Carbon disulfide
18) Isopropyl alcohol
19) Allyl chloride (3-chloropropene)
20) Acetonitrile
21) Methylene chloride
22) trans-1,2-Dichloroethene
23) Methyl tert-butyl ether
24) Acrylonitrile
25) Hexane
26) 1,1 -Dichloroethane
27) Vinyl acetate
28) cis-1,2-Dichloroethylene
29) Methyl ethyl ketone (2-Butanone)
30) Ethyl acetate
31) Chloroform
32) Tetrahydrofuran
33) 1,1,1-Trichloroethane
34) Cyclohexane
35) Carbon tetrachloride
37) Benzene
38) 2,2,4-trimethylpentane
39) 1,2-Dichloroethane
40) Heptane
41) Trichloroethene
42) 1,2-Dichloropropane
43) Methyl methacrylate
44) 1,4-Dioxane
45) Bromodichloromethane
46) cis-1,3-Dichloropropene
47) Methyl isobutyl ketone
49) Toluene
50) trans-1,3-Dichloropropene
51) 1,1,2-Trichloroethane
52) Tetrachloroethene
53) 2-Hexanone (Methyl butyl ketone)
54) Dibromochloromethane
55) 1,2-Dibromoethane
56) Chlorobenzene
57) Ethylbenzene
58) 1,1,1,2-Tetrachloroethane
59) m-/p-Xylenes
60) o-Xylene
61) Styrene
62) Bromoform
63) 1,1,2,2-Tetrachloroethane
0.4ppbv
to15stdA
0.157
1.57
1.40
0.248
0.323
0.301
0.890
0.193
0.858
2.16
0.103
0.0692
0.978
0.503
0.629
0.922
0.535
0.105
0.533
0.414
0.850
0.104
0.292
0.501
0.634
0.384
0.0899
0.0875
0.970
0.105
1.33
0.315
1.59
0.267
0.344
0.202
0.0675
0.314
0.0928
0.0691
0.108
0.404
0.158
0.234
0.532
0.215
0.266
0.495
0.187
0.768
0.580
0.675
0.811
0.441
0.674
0.663
0.540
0.740
0.475
1 ppbv to1 5
std A
0.207
1.91
1.68
0.285
0.391
0.377
1.06
0.207
1.05
2.51
0.169
0.0984
1.15
0.579
0.669
1.00
0.600
0.133
0.254
0.471
0.511
0.974
0.147
0.341
0.574
0.875
0.450
0.120
0.105
1.16
0.126
1.61
0.347
1.88
0.325
0.429
0.235
0.0813
0.378
0.107
0.0979
0.115
0.478
0.205
0.308
0.628
0.247
0.317
0.561
0.260
0.893
0.682
0.793
0.955
0.515
0.764
0.787
0.687
0.877
0.586
2ppbv to1 5
std A
0.196
1.92
1.72
0.280
0.415
0.369
1.04
0.190
0.976
2.40
0.132
0.0968
1.15
0.594
0.670
0.958
0.565
0.129
0.213
0.418
0.506
0.990
0.159
0.347
0.570
0.916
0.459
0.126
0.113
1.13
0.128
1.54
0.382
1.83
0.333
0.412
0.241
0.0788
0.352
0.101
0.102
0.119
0.468
0.209
0.313
0.633
0.264
0.314
0.545
0.310
0.870
0.679
0.778
0.933
0.499
0.754
0.764
0.692
0.862
0.569
5ppbvto-15
std A
0.178
1.71
1.55
0.248
0.353
0.309
0.930
0.164
0.904
2.07
0.112
0.0826
0.970
0.531
0.576
0.885
0.523
0.121
0.195
0.372
0.449
0.878
0.145
0.302
0.491
0.839
0.419
0.112
0.0975
1.03
0.110
1.37
0.335
1.60
0.291
0.379
0.215
0.0704
0.318
0.0919
0.0898
0.108
0.417
0.194
0.281
0.606
0.254
0.300
0.497
0.293
0.795
0.638
0.729
0.884
0.466
0.721
0.716
0.653
0.780
0.534
10ppbvto-
15 std A
0.176
1.77
1.60
0.259
0.366
0.321
0.928
0.167
0.914
2.05
0.107
0.0846
0.960
0.532
0.556
0.873
0.491
0.119
0.175
0.365
0.448
0.872
0.151
0.302
0.509
0.862
0.422
0.115
0.0957
1.04
0.115
1.38
0.333
1.58
0.295
0.376
0.216
0.0710
0.320
0.0931
0.0919
0.109
0.431
0.199
0.281
0.625
0.274
0.311
0.521
0.323
0.829
0.679
0.767
0.932
0.478
0.766
0.757
0.697
0.822
0.548
20ppbv to-
15 std A
0.182
1.88
1.71
0.270
0.389
0.342
1.01
0.181
0.969
2.12
0.0942
0.0876
1.03
0.575
0.603
0.948
0.526
0.130
0.176
0.386
0.483
0.949
0.163
0.331
0.541
0.929
0.457
0.127
0.101
1.12
0.123
1.47
0.353
1.68
0.315
0.397
0.233
0.0744
0.340
0.100
0.100
0.118
0.468
0.220
0.299
0.629
0.269
0.311
0.510
0.330
0.805
0.683
0.779
0.954
0.464
0.796
0.767
0.715
0.809
0.542
SOppbv to-
15 std A
0.153
1.65
1.51
0.230
0.337
0.293
0.912
0.161
0.851
1.81
0.0970
0.0794
0.908
0.513
0.534
0.857
0.472
0.117
0.155
0.341
0.438
0.872
0.144
0.297
0.484
0.833
0.413
0.117
0.0906
1.02
0.112
1.31
0.319
1.47
0.284
0.355
0.207
0.0659
0.299
0.0866
0.0895
0.106
0.416
0.197
0.260
0.589
0.251
0.290
0.464
0.300
0.721
0.636
0.726
0.896
0.414
0.752
0.723
0.689
0.733
0.501
Average RF
0.178
1.77
1.59
0.260
0.368
0.330
0.968
0.180
0.932
2.16
0.116
0.0855
1.02
0.547
0.605
0.921
0.530
0.122
0.195
0.412
0.464
0.912
0.145
0.316
0.524
0.841
0.429
0.115
0.0988
1.07
0.117
1.43
0.340
1.66
0.301
0.385
0.221
0.0727
0.332
0.0960
0.0914
0.112
0.440
0.197
0.282
0.606
0.253
0.301
0.513
0.286
0.812
0.654
0.750
0.909
0.468
0.747
0.740
0.668
0.803
0.537
%RSD
10.8%
7.74%
7.42%
7.50%
8.88%
10.0%
7.14%
9.52%
7.63%
10.9%
22.5%
11.8%
9.31%
6.50%
8.86%
5.64%
8.12%
7.83%
18.2%
16.5%
7.91%
6.26%
13.4%
7.31%
7.10%
11.7%
6.43%
10.8%
8.94%
6.52%
7.48%
7.76%
6.75%
8.90%
7.94%
7.85%
6.80%
7.84%
8.03%
7.08%
12.0%
4.91%
6.90%
9.88%
9.90%
5.96%
7.74%
6.00%
6.31%
17.2%
7.25%
5.90%
5.55%
5.61%
7.25%
5.20%
5.67%
8.87%
6.96%
7.12%
EA# 0211-49 Page 53 of 106
-------
Sample ID
0.4ppbvto15stdA
1ppbvto15stdA
2ppbv to15 std A
5ppbv to-15 std A
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
LID
0.4
1
2
5
10
20
30
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
Response Factor Report
Acq. Date
02/11/11 14:40
02/11/11 15:23
02/11/11 16:42
02/10/11 20:50
02/10/11 21:32
02/10/11 22:19
02/10/11 23:10
Inst:
Lurch
Curve: L021111A.M
# Name
64) 4-Ethyltoluene
65) 2-Chlorotoluene
66) 1,3,5-Trimethylbenzene
67) 1,2,4-Trimethylbenzene
68) 1,3-Dichlorobenzene
69) 1,4-Dichlorobenzene
70) Benzyl chloride
71) 1,2-Dichlorobenzene
72) 1,2,4-Trichlorobenzene
73) Hexachlorobutadiene
74) Naphthalene
= %RSD >30 and <40, two allowed
0.4ppbv
to15stdA
1.05
0.911
1.05
0.932
0.599
0.633
0.474
0.675
0.158
0.474
0.532
1 ppbv to1 5
std A
1.41
1.07
1.18
1.17
0.831
0.835
0.738
0.897
0.435
0.815
1.28
2ppbv to1 5
std A
1.33
1.05
1.17
1.13
0.818
0.824
0.750
0.838
0.461
0.651
1.31
5ppbvto-15
std A
1.27
1.00
1.07
1.05
0.743
0.747
0.717
0.755
0.338
0.478
0.953
10ppbvto-
15 std A
1.38
1.04
1.10
1.10
0.788
0.801
0.774
0.780
0.432
0.490
1.15
20ppbv to-
15 std A
1.40
1.06
1.11
1.10
0.794
0.802
0.803
0.773
0.493
0.499
1.29
SOppbv to-
15 std A
1.31
1.01
1.01
1.03
0.719
0.731
0.771
0.698
0.503
0.456
1.29
Average RF
1.31
1.02
1.10
1.07
0.756
0.767
0.718
0.774
0.403
0.552
1.11
%RSD
9.54%
5.44%
5.46%
7.27%
10.5%
9.20%
15.5%
9.91%
30.0%
24.1%
25.7%
EA# 0211-49 Page 54 of 106
-------
Relative Retention Time Report
Sample ID
0.4ppbvto15stdA
1 ppbv to15 std A
2ppbv to15 std A
5ppbvto-15stdA
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
# Name
2) Pnopylene
3) Freon12(CCI2F2)
4) Freon 114(C2CI2F4)
5) Chloromethane
6) Chloroethene (Vinyl chloride)
7) 1,3-Butadiene
8) Bromomethane
9) Chloroethane
10) Bromoethene (Vinyl bromide)
11) Freon 11 (CCI3F)
12) Ethanol
13) Acrolein
14) Freon 113 (C2CI3F3)
15) 1,1-Dichloroethene
16) Acetone
17) Carbon disulfide
18) Isopropyl alcohol
19) Allyl chloride (3-chloropropene)
20) Acetonitrile
21) Methylene chloride
22) trans-1,2-Dichloroethene
23) Methyl tert-butyl ether
24) Acrylonitrile
25) Hexane
26) 1,1-Dichloroethane
27) Vinyl acetate
28) cis-1,2-Dichloroethylene
29) Methyl ethyl ketone (2-Butanone)
30) Ethyl acetate
31) Chloroform
32) Tetrahydrofuran
33) 1,1,1-Trichloroethane
34) Cyclohexane
35) Carbon tetrachloride
37) Benzene
38) 2,2,4-trimethylpentane
39) 1,2-Dichloroethane
40) Heptane
41) Trichloroethene
42) 1,2-Dichloropropane
43) Methyl methacrylate
44) 1,4-Dioxane
45) Bromodichloromethane
46) cis-1,3-Dichloropropene
47) Methyl isobutyl ketone
49) Toluene
50) trans-1,3-Dichloropropene
51) 1,1,2-Trichloroethane
52) Tetrachloroethene
53) 2-Hexanone (Methyl butyl ketone)
54) Dibromochloromethane
55) 1,2-Dibromoethane
56) Chlorobenzene
57) Ethylbenzene
58) 1,1,1,2-Tetrachloroethane
59) m-/p-Xylenes
60) o-Xylene
61) Styrene
62) Bromoform
63) 1,1,2,2-Tetrachloroethane
64) 4-Ethyltoluene
LID
0.4
1
2
5
10
20
30
0.4ppbv
to15stdA
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.64
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.80
0.81
0.86
0.86
0.96
0.97
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.01
1.01
1.01
1.03
1.04
1.05
1.07
1.08
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
1 ppbv to1 5
std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.80
0.81
0.86
0.86
0.96
0.97
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.00
1.01
1.01
1.03
1.04
1.05
1.07
1.08
Acq. Date
02/11/11
02/11/11
02/11/11
02/10/11
02/10/11
02/10/11
02/10/11
2ppbv to1 5
std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.79
0.81
0.86
0.86
0.96
0.97
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.01
1.01
1.01
1.03
1.04
1.05
1.07
1.08
14:40
15:23
16:42
20:50
21:32
22:19
23:10
5ppbvto-15
std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.73
0.77
0.77
0.79
0.80
0.85
0.86
0.96
0.96
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.01
1.01
1.01
1.03
1.04
1.05
1.07
1.08
Inst:
Curve:
10ppbvto-
15 std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.79
0.81
0.86
0.86
0.96
0.96
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.00
1.01
1.01
1.03
1.04
1.05
1.07
1.08
Lurch
L021111A.
20ppbv to-
15 std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.79
0.81
0.86
0.86
0.96
0.96
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.01
1.01
1.01
1.03
1.04
1.05
1.07
1.08
JM
SOppbv to-
15 std A
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.79
0.81
0.85
0.86
0.96
0.96
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.00
1.01
1.01
1.03
1.04
1.05
1.07
1.08
Average
RRT
0.33
0.34
0.37
0.38
0.40
0.41
0.47
0.49
0.53
0.54
0.60
0.63
0.63
0.64
0.66
0.67
0.69
0.71
0.73
0.74
0.77
0.77
0.79
0.81
0.86
0.86
0.96
0.96
0.97
1.01
1.00
1.03
1.03
1.05
0.95
0.94
0.96
0.97
1.03
1.06
1.06
1.07
1.09
1.14
1.15
0.89
0.92
0.94
0.94
0.95
0.96
0.97
1.00
1.01
1.01
1.01
1.03
1.04
1.05
1.07
1.08
EA# 0211-49 Page 55 of 106
-------
Sample ID
0.4ppbvto15stdA
1 ppbv to15 std A
2ppbv to15 std A
5ppbvto-15stdA
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
Relative Retention Time Report
Inst:
Curve:
LID
0.4
1
2
5
10
20
30
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
Acq. Date
02/11/11 14:40
02/11/11 15:23
02/11/11 16:42
02/10/11 20:50
02/10/11 21:32
02/10/11 22:19
02/10/11 23:10
Lurch
L021111A.M
# Name
65) 2-Chlorotoluene
66) 1,3,5-Trimethylbenzene
67) 1,2,4-Trimethylbenzene
68) 1,3-Dichlorobenzene
69) 1,4-Dichlorobenzene
70) Benzyl chloride
71) 1,2-Dichlorobenzene
72) 1,2,4-Trichlorobenzene
73) Hexachlorobutadiene
74) Naphthalene
= RRT outside 0.06 unit RRT window
0.4ppbv
to15stdA
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
1 ppbv to1 5
std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
2ppbv to1 5
std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
5ppbvto-15
std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
10ppbvto-
15 std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
20ppbv to-
15 std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
SOppbv to-
15 std A
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
Average
RRT
1.08
1.08
1.10
1.11
1.12
1.12
1.13
1.20
1.21
1.22
EA# 0211-49 Page 56 of 106
-------
Sample ID
0.4ppbvto15stdA
1 ppbv to15 std A
2ppbv to15 std A
5ppbvto-15stdA
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
Internal Standards Retention Time Report
LID
0.4
1
2
5
10
20
30
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
Acq. Date
02/11/11 14:40
02/11/11 15:23
02/11/11 16:42
02/10/11 20:50
02/10/11 21:32
02/10/11 22:19
02/10/11 23:10
Inst:
Lurch
Curve: L021111A.M
# Name
1) Bromochloromethane (IS)
36) 1,4-Difluorobenzene (IS)
48) Chlorobenzene-d5 (IS)
= ISTD retention time outside of 20 sec (0.33 min) window
0.4ppbv
to15stdA
12.43
14.23
18.60
1 ppbv to1 5
std A
12.43
14.23
18.61
2ppbv to1 5
std A
12.43
14.23
18.60
5ppbvto-15
std A
12.40
14.20
18.59
10ppbvto-
15 std A
12.40
14.20
18.60
20ppbv to-
15 std A
12.40
14.20
18.59
SOppbv to-
15 std A
12.41
14.21
18.59
Average
12.41
14.21
18.60
EA# 0211-49 Page 57 of 106
-------
Sample ID
0.4ppbvto15stdA
1 ppbv to15 std A
2ppbv to15 std A
5ppbvto-15stdA
10ppbvto-15stdA
20ppbvto-15stdA
30ppbvto-15stdA
LID
0.4
1
2
5
10
20
30
FileName
L1100398.D
L1100399.D
L1100400.D
L1100386.D
L1100387.D
L1100388.D
L1100389.D
Inst:
Internal Standards Area Report
Acq. Date
02/11/11 14:40
02/11/11 15:23
02/11/11 16:42
02/10/11 20:50
02/10/11 21:32
02/10/11 22:19
02/10/11 23:10
Lurch
Curve: L021111A.M
# Name
1) Bromochloromethane (IS)
36) 1,4-Difluorobenzene (IS)
48) Chlorobenzene-d5 (IS)
0.4ppbv
to15stdA
873,087
2,565,767
1,934,152
1ppbvto15 2ppbvto15 5ppbvto-15 10ppbvto- 20ppbvto- SOppbvto-
stdA std A std A 15 std A 15 std A 15 std A Average
763,953 786,578 857,643 839,895 835,157 981,377 848,241
2,258,222 2,307,213 2,579,573 2,550,701 2,566,607 3,055,944 2,554,861
1,764,107 1,820,826 1,951,915 1,906,841 2,123,122 2,445,621 1,992,369
= ISTD response outside of 40% window
EA# 0211-49 Page 58 of 106
-------
Tag Values
# Compound Name
2) Propylene
3) Freon 12 (CCI2F2)
4) Freon 114 (C2CI2F4)
5) Chloromethane
6) Chloroethene (Vinyl chloride)
7) 1,3-Butadiene
8) Bromomethane
9) Chloroethane
10) Bromoethene (Vinyl bromide)
11) Freon 11 (CCI3F)
12) Ethanol
13) Acrolein
14) Freon 113 (C2CI3F3)
15) 1,1-Dichloroethene
16) Acetone
17) Carbon disulfide
18) Isopropyl alcohol
19) Allyl chloride (3-chloropropene)
20) Acetonitrile
21) Methylene chloride
22) trans-1,2-Dichloroethene
23) Methyl tert-butyl ether
24) Acrylonitrile
25) Hexane
26) 1,1-Dichloroethane
27) Vinyl acetate
28) cis-1,2-Dichloroethylene
29) Methyl ethyl ketone (2-Butanone)
30) Ethyl acetate
31) Chloroform
32) Tetrahydrofuran
33) 1,1,1-Trichloroethane
34) Cyclohexane
35) Carbon tetrachloride
37) Benzene
38) 2,2,4-trimethylpentane
39) 1,2-Dichloroethane
40) Heptane
41) Trichloroethene
42) 1,2-Dichloropropane
43) Methyl methacrylate
44) 1,4-Dioxane
45) Bromodichloromethane
46) cis-1,3-Dichloropropene
47) Methyl isobutyl ketone
49) Toluene
50) trans-1,3-Dichloropropene
51) 1,1,2-Trichloroethane
52) Tetrachloroethene
53) 2-Hexanone (Methyl butyl ketone)
54) Dibromochloromethane
55) 1,2-Dibromoethane
56) Chlorobenzene
57) Ethylbenzene
58) 1,1,1,2-Tetrachloroethane
59) m-/p-Xylenes
60) o-Xylene
61) Styrene
SOppbv
33.0
29.4
29.4
29.7
29.4
30.9
29.4
29.4
30.6
30.3
32.1
30.9
30.9
30.9
31.8
31.2
33.0
31.8
27.9
31.2
30.9
31.2
30.3
31.8
30.6
31.5
30.9
31.8
31.2
31.2
32.1
30.6
30.9
30.6
30.6
29.4
30.6
31.2
30.9
30.9
30.3
30.9
30.9
31.8
31.5
31.2
29.1
31.2
30.6
32.4
32.4
30.6
31.5
31.2
31.2
61.2
31.8
31.2
20ppbv
22.0
19.6
19.6
19.8
19.6
20.6
19.6
19.6
20.4
20.2
21.4
20.6
20.6
20.6
21.2
20.8
22.0
21.2
18.6
20.8
20.6
20.8
20.2
21.2
20.4
21.0
20.6
21.2
20.8
20.8
21.4
20.4
20.6
20.4
20.4
19.6
20.4
20.8
20.6
20.6
20.2
20.6
20.6
21.2
21.0
20.8
19.4
20.8
20.4
21.6
21.6
20.4
21.0
20.8
20.8
40.8
21.2
20.8
10ppbv
11.0
9.80
9.80
9.90
9.80
10.3
9.80
9.80
10.2
10.1
10.7
10.3
10.3
10.3
10.6
10.4
11.0
10.6
9.30
10.4
10.3
10.4
10.1
10.6
10.2
10.5
10.3
10.6
10.4
10.4
10.7
10.2
10.3
10.2
10.2
9.80
10.2
10.4
10.3
10.3
10.1
10.3
10.3
10.6
10.5
10.4
9.70
10.4
10.2
10.8
10.8
10.2
10.5
10.4
10.4
20.4
10.6
10.4
Sppbv
5.50
4.90
4.90
4.95
4.90
5.15
4.90
4.90
5.10
5.05
5.35
5.15
5.15
5.15
5.30
5.20
5.50
5.30
4.65
5.20
5.15
5.20
5.05
5.30
5.10
5.25
5.15
5.30
5.20
5.20
5.35
5.10
5.15
5.10
5.10
4.90
5.10
5.20
5.15
5.15
5.05
5.15
5.15
5.30
5.25
5.20
4.85
5.20
5.10
5.40
5.40
5.10
5.25
5.20
5.20
10.2
5.30
5.20
4ppbv
4.40
3.92
3.92
3.96
3.92
4.12
3.92
3.92
4.08
4.04
4.28
4.12
4.12
4.12
4.24
4.16
4.40
4.24
3.72
4.16
4.12
4.16
4.04
4.24
4.08
4.20
4.12
4.24
4.16
4.16
4.28
4.08
4.12
4.08
4.08
3.92
4.08
4.16
4.12
4.12
4.04
4.12
4.12
4.24
4.20
4.16
3.88
4.16
4.08
4.32
4.32
4.08
4.20
4.16
4.16
8.16
4.24
4.16
2ppbv
2.20
1.96
1.96
1.98
1.96
2.06
1.96
1.96
2.04
2.02
2.14
2.06
2.06
2.06
2.12
2.08
2.20
2.12
1.86
2.08
2.06
2.08
2.02
2.12
2.04
2.10
2.06
2.12
2.08
2.08
2.14
2.04
2.06
2.04
2.04
1.96
2.04
2.08
2.06
2.06
2.02
2.06
2.06
2.12
2.10
2.08
1.94
2.08
2.04
2.16
2.16
2.04
2.10
2.08
2.08
4.08
2.12
2.08
1ppbv
1.10
0.980
0.980
0.990
0.980
1.03
0.980
0.980
1.02
1.01
1.07
1.03
1.03
1.03
1.06
1.04
1.10
1.06
0.930
1.04
1.03
1.04
1.01
1.06
1.02
1.05
1.03
1.06
1.04
1.04
1.07
1.02
1.03
1.02
1.02
0.980
1.02
1.04
1.03
1.03
1.01
1.03
1.03
1.06
1.05
1.04
0.970
1.04
1.02
1.08
1.08
1.02
1.05
1.04
1.04
2.04
1.06
1.04
0.4ppbv
0.44
0.392
0.392
0.396
0.392
0.412
0.392
0.392
0.408
0.404
0.428
0.412
0.412
0.412
0.424
0.416
0.44
0.424
0.372
0.416
0.412
0.416
0.404
0.424
0.408
0.42
0.412
0.424
0.416
0.416
0.428
0.408
0.412
0.408
0.408
0.392
0.408
0.416
0.412
0.412
0.404
0.412
0.412
0.424
0.42
0.416
0.388
0.416
0.408
0.432
0.432
0.408
0.42
0.416
0.416
0.816
0.424
0.416
0.2ppbv
EA# 0211-49 Page 59 of 106
-------
Tag Values
# Compound Name 30ppbv 20ppbv 10ppbv Sppbv 4ppbv 2ppbv 1ppbv 0.4ppbv 0.2ppbv
62) Bromoform 30.6 20.4 10.2 5.10 4.08 2.04 1.02 0.408
63) 1,1,2,2-Tetrachloroethane 31.8 21.2 10.6 5.30 4.24 2.12 1.06 0.424
64) 4-Ethyltoluene 31.2 20.8 10.4 5.20 4.16 2.08 1.04 0.416
65) 2-Chlorotoluene 29.1 19.4 9.70 4.85 3.88 1.94 0.970 0.388
66) 1,3,5-Trimethylbenzene 30.9 20.6 10.3 5.15 4.12 2.06 1.03 0.412
67) 1,2,4-Trimethylbenzene 31.2 20.8 10.4 5.20 4.16 2.08 1.04 0.416
68) 1,3-Dichlorobenzene 31.5 21.0 10.5 5.25 4.20 2.10 1.05 0.42
69) 1,4-Dichlorobenzene 30.6 20.4 10.2 5.10 4.08 2.04 1.02 0.408
70) Benzyl chloride 31.5 21.0 10.5 5.25 4.20 2.10 1.05 0.42
71) 1,2-Dichlorobenzene 30.9 20.6 10.3 5.15 4.12 2.06 1.03 0.412
72) 1,2,4-Trichlorobenzene 30.6 20.4 10.2 5.10 4.08 2.04 1.02 0.408
73) Hexachlorobutadiene 30.9 20.6 10.3 5.15 4.12 2.06 1.03 0.412
74) Naphthalene 31.5 21.0 10.5 5.25 4.20 2.10 1.05 0.42
EA# 0211-49 Page 60 of 106
-------
Response Factor Report Lurch
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
Calibration Files
0.4
5
1) I
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
36) I
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
48) I
49)
50)
51)
52)
53)
54)
55)
56)
57)
=L1100398.D 1.0
=L1100386.D 10
Compound
=L1100399.D 2
=L1100387.D 20
0.4 1.0 2 5
Bromochloromethane (I
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-c
Acetonitrile
Methylene chloride
trans-1, 2-Dichloroe
Methyl tert -butyl e
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroeth
Methyl ethyl ketone
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroetha
Cyclohexane
Carbon tetrachlorid
1, 4-Dif luorobenzene
Benzene
2, 2,4-trimethylpent
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethan
cis-1, 3-Dichloropro
Methyl isobutyl ket
0.
1.
1.
0.
0.
0.
0.
0.
0.
2.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
1.
0.
1.
(
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
Chlorobenzene-d5 (IS)
Toluene
trans-1, 3-Dichlorop
1,1,2 -Trichloroetha
Tetrachloroethene
2-Hexanone (Methyl
Dibromochloromethan
1 , 2 -Dibromoethane
Chlorobenzene
Ethylbenzene
0.
0.
0.
0.
0.
0.
0.
0.
0.
157
566
397
248
323
301
890
193
858
156
103
069
978
503
629
922
535
105
533
414
850
104
292
501
634
384
090
088
970
105
332
315
589
267
344
202
067
314
093
069
108
404
158
234
532
215
266
495
187
768
580
675
811
0
1
1
0
0
0
1
0
1
2
0
0
1
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.207
.907
.681
.285
.391
.377
.065
.207
.049
.513
.169
.098
.148
.579
.669
.001
.600
.133
.254
.471
.511
.974
.147
.341
.574
.875
.450
.120
.105
.159
.126
.607
.347
.883
.325
.429
.235
.081
.378
.107
.098
.115
.478
.205
.308
.628
.247
.317
.561
.260
.893
.682
.793
.955
0.196
1.923
1.716
0.280
0.415
0.369
1.037
0.190
0.976
2.401
0.132
0.097
1.151
0.594
0.670
0.958
0.565
0.129
0.213
0.418
0.506
0.990
0.159
0.347
0.570
0.916
0.459
0.126
0.113
1.128
0.128
1.536
0.382
1.835
0.333
0.412
0.241
0.079
0.352
0.101
0.102
0.119
0.468
0.209
0.313
0.633
0.264
0.314
0.545
0.310
0.870
0.679
0.778
0.933
-TQTTl -
0.178
1.710
1.545
0.248
0.353
0.309
0.915
0.164
0.904
2.071
0.112
0.083
0.970
0.531
0.576
0.885
0.523
0.121
0.195
0.372
0.449
0.878
0.145
0.302
0.491
0.839
0.419
0.112
0.097
1.027
0.110
1.373
0.335
1.596
T C'p'n
0.291
0.379
0.215
0.070
0.318
0.092
0.090
0.108
0.417
0.194
0.281
T C'p'n
0.606
0.254
0.300
0.497
0.293
0.795
0.638
0.729
0.884
=L1100400.D
=L1100388.D
10 20
0.176
1.768
1.599
0.259
0.366
0.321
0.928
0.167
0.914
2.046
0.107
0.085
0.960
0.532
0.556
0.873
0.491
0.119
0.175
0.365
0.448
0.872
0.151
0.302
0.509
0.862
0.422
0.115
0.096
1.036
0.115
1.376
0.333
1.585
0.295
0.376
0.216
0.071
0.320
0.093
0.092
0.109
0.431
0.199
0.281
0.625
0.274
0.311
0.521
0.323
0.829
0.679
0.767
0.932
0.182
1.879
1.710
0.270
0.389
0.342
1.015
0.181
0.969
2.119
0.094
0.088
1.034
0.575
0.603
0.948
0.526
0.130
0.176
0.386
0.483
0.949
0.163
0.331
0.541
0.929
0.457
0.127
0.101
1.121
0.123
1.474
0.353
1.676
0.315
0.397
0.233
0.074
0.340
0.100
0.100
0.118
0.468
0.220
0.299
0.629
0.269
0.311
0.510
0.330
0.805
0.683
0.779
0.954
Avg
0.178
1.772
1.594
0.260
0.368
0.330
0.966
0.180
0.932
2.159
0.116
0.085
1.021
0.547
0.605
0.921
0.530
0.122
0.195
0.412
0.464
0.912
0.145
0.316
0.524
0.841
0.429
0.115
0.099
1.066
0.117
1.430
0.340
1.662
0.301
0.385
0.221
0.073
0.332
0.096
0.091
0.112
0.440
0.197
0.282
0.606
0.253
0.301
0.513
0.286
0.812
0.654
0.750
0.909
%RSD
10.80
7.74
7.42
7.50
8.88
10.03
7.32
9.52
7.63
10.88
22.53
11.77
9.31
6.50
8.86
5.64
8.12
7.83
18.18
16.47
7.91
6.26
13.37
7.31
7.10
11.69
6.43
10.76
8.94
6.52
7.48
7.76
6.75
8.90
7.94
7.85
6.80
7.84
8.03
7.08
11.97
4.91
6.90
9.88
9.90
5.96
7.74
6.00
6.31
17.24
7.25
5.90
5.55
5.61
(#) = Out of Range ### Number of
L021111A.M Fri Feb 11 19:37:46 2011
###
Page 1
-------
Method
Title
Last Update
Response via
Response Factor Report Lurch
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
Calibration Files
0.4 =L1100398.D 1.0
5 =L1100386.D 10
Compound
58)
59)
60)
61)
62)
63)
64)
65)
66)
67)
68)
69)
70)
71)
72)
73)
74)
1,1,1,2 -Tetrachloro
m-/p-Xylenes
o-Xylene
Styrene
Bromoform
1,1,2,2 -Tetrachloro
4 -Ethyl toluene
2-Chlorotoluene
1,3, 5 -Trimethylbenz
1,2, 4 -Trimethylbenz
1 , 3 -Dichlorobenzene
1 , 4 -Dichlorobenzene
Benzyl chloride
1 , 2 -Dichlorobenzene
1,2, 4 -Trichlorobenz
Hexachlorobutadiene
Naphthalene
0.
0.
0.
0.
0.
0.
1.
0.
1.
0.
0.
0.
0.
0.
0.
0.
0.
=L1100399.D 2
=L1100387.D 20
0.4 1.0 2 5
441
674
663
540
740
475
047
911
054
932
599
633
474
675
158
474
532
0.515
0.764
0.787
0.687
0.877
0.586
1.406
1.071
1.180
1.172
0.831
0.835
0.738
0.897
0.435
0.815
1.284
0.499
0.754
0.764
0.692
0.862
0.569
1.332
1.054
1.168
1.131
0.818
0.824
0.750
0.838
0.461
0.651
1.307
0.466
0.721
0.716
0.653
0.780
0.534
1.272
1.002
1.070
1.047
0.743
0.747
0.717
0.755
0.338
0.478
0.953
=L1100400.D
=L1100388.D
10 20
0.478
0.766
0.757
0.697
0.822
0.548
1.376
1.044
1.095
1.102
0.788
0.801
0.774
0.780
0.432
0.490
1.148
0.464
0.796
0.767
0.715
0.809
0.542
1.404
1.065
1.111
1.096
0.794
0.802
0.803
0.773
0.493
0.499
1.288
0
0
0
0
0
0
1
1
1
1
0
0
0
0
0
0
1
Avg
.468
.747
.740
.668
.803
.537
.307
.022
.099
.074
.756
.767
.718
.774
.403
.552
.114
%RSD
7.25
5.20
5.67
8.87
6.96
7.12
9.54
5.44
5.46
7.27
10.54
9.20
15.46
9.91
29.98
24.11
25.71
(#) = Out of Range ### Number of
L021111A.M Fri Feb 11 19:37:46 2011
###
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100398.D Vial: 1
Acq On : 11 Feb 2011 2:40 pm Operator: tdd/cld
Sample : 0.4ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, lOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:08 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 17:14:05 2011
Response via : Initial Calibration
TIC: L1100398. D
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
0
II
4.00 5.00 6.00 7.00 8.00 9.00 10.001,
L1100398.D L021111A.M Fri Feb 11 19:08:53 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100398.D Vial: 1
Acq On : 11 Feb 2011 2:40 pm Operator: tdd/cld
Sample : 0.4ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, lOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:08 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 12:03:10 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.43
.23
.60
130
114
117
873087
2565767
1934152
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
^vatnG T ^ T~ln fVil ovonvon^Ti^
L.-LGL1J.O -L , J U J_L.ll-Ll_)-L l_)£J-L 1_)£JC11C
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
10
10
10
11
12
12
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.15
.25
.57
.75
.03
.11
.89
.15
.58
.67
.44
.90
.84
.91
.25
.35
.60
.80
.04
.15
.63
.59
.89
.02
.64
.74
.91
.00
.01
.57
.45
.82
.77
.05
.51
.43
.70
.78
.59
.04
.14
.20
.45
.18
.39
.64
.19
.46
.52
.75
.95
.13
.63
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
5352
47673
42527
7631
9824
9643
27092
5877
27180
67648
3412
2216
31294
16102
20717
29785
18298
3472
21677
17227
13235
27471
3264
9606
15876
20668
12275
2961
2829
31351
3486
42229
10066
50352
23385
29012
17754
6038
27860
8221
6008
9526
35812
14398
21150
38457
14484m
19243
35062
14046
57639
41134
49258
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.35
.31
.31
.34
.31
.35
.33
.38
.34
.37
.36
.30
.36
.34
.42
.38
.40
.32
.06
.49
.33
.36
.27
.36
.36
.30
.33
.31
.35
.34
.36
.35
.35
.36
.33
.31
.34
.35
.36
.36
.29
.36
.35
.31
.32
.33
.31
.34
.36
.27
.38
.34
.35
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.02
0.02
0.02
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
98
95
97
89
88
95
41
99
100
1
100
100
74
94
78
91
91
69
93
67
91
91
89
94
99
69
87
78
99
87
95
93
100
97
89
98
84
94
98
95
81
97
99
96
98
„ „ iManual Int. "II" (CLD)|
97
61
99
99
82
(#) = qualifier out of range (m) =
L1100398.D L021111A.M Fri Feb 11 19:08:51 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100398.D Vial: 1
Acq On : 11 Feb 2011 2:40 pm Operator: tdd/cld
Sample : 0.4ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, lOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:08 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 12:03:10 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.71 91 58623 0.35 PPBV 93
58) 1,1,1,2-Tetrachloroethane 18.73 131 31871 0.36 PPBV # 76
59) m-/p-Xylenes 18.83 91 95627 0.69 PPBV 91
60) o-Xylene 19.25 91 48886 0.36 PPBV 88
61) Styrene 19.28 104 39054 0.32 PPBV # 92
62) Bromoform 19.51 173 52456 0.36 PPBV # 95
63) 1,1,2,2-Tetrachloroethane 19.92 83 35003 0.36 PPBV 99
64) 4-Ethyltoluene 20.04 105 75685 0.32 PPBV 99
65) 2-Chlorotoluene 20.07 91 61435 0.33 PPBV 94
66) 1,3,5-Trimethylbenzene 20.09 105 75467m 0.38 PPBV
67) 1,2,4-Trimethylbenzene 20.42 105 67359 0.34 PPBV 98
68) 1,3-Dichlorobenzene 20.71 146 43740 0.33 PPBV 98
69) 1,4-Dichlorobenzene 20.78 146 44859 0.33 PPBV 93
70) Benzyl chloride 20.89 91 34614 0.27 PPBV 99
71) 1,2-Dichlorobenzene 21.09 146 48335 0.35 PPBV 95
72) 1,2,4-Trichlorobenzene 22.40 180 11220 0.18 PPBV 98
73) Hexachlorobutadiene 22.44 225 33956 0.37 PPBV 98
74) Naphthalene 22.65 128 38842 0.22 PPBV 95
Manual Int. "II" (CLD)||
L1100398.D L021111A.M
Fri Feb 11 19:08:52 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100399.D Vial: 1
Acq On : 11 Feb 2011 3:23 pm Operator: tdd/cld
Sample : Ippbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 16:19 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:10:11 2011
Response via : Initial Calibration
TIC: L1100399. D
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
4.00 5.00 6.00 7.00 8.00 9.00 10.001,
L1100399.D L021111A.M Fri Feb 11 19:10:39 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100399.D Vial: 1
Acq On : 11 Feb 2011 3:23 pm Operator: tdd/cld
Sample : Ippbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 16:19 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 15:44:07 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.43
.23
.61
130
114
117
763953
2258222
1764107
11.
11.
11.
24
93
13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
10
10
10
11
12
12
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.15
.24
.57
.75
.03
.11
.89
.15
.58
.67
.43
.89
.85
.91
.25
.35
.59
.81
.04
.15
.62
.58
.88
.01
.65
.73
.92
.00
.02
.57
.45
.82
.78
.05
.50
.43
.70
.78
.60
.03
.13
.21
.45
.17
.39
.64
.19
.46
.51
.74
.95
.12
.64
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
15442
127024
111976
19147
26073
26425
70915
13773
72734
172541
12255
6886
80367
40546
48182
70781
44894
9609
16083
33279
35797
68843
10064
24590
39769
62423
31489
8638
7457
81937
9190
111442
24327
130531
62711
79552
45459
16002
73641
20808
18725
22448
93221
41176
61224
103481
37988
52246
90647
44479
152850
110269
131933
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
0.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
1.
0.
1.
1.
1.
1.
1.
1.
28
04
03
09
05
18
08
12
13
17
56
20
15
07
17
11
23
12
81
15
13
10
02
13
11
08
06
09
11
12
15
14
04
16
09
07
08
16
18
13
09
03
11
09
15
06
96
07
10
00
19
05
10
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.02
0.02
0.01
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
99
95
100
99
97
96
81
97
98
31
94
100
71
89
99
91
94
87
94
82
95
98
89
98
98
67
96
90
97
93
96
1
99
98
91
98
97
99
99
93
97
98
95
98
96
91
94
97
97
98
100
98
(#) = qualifier out of range (m) =
L1100399.D L021111A.M Fri Feb 11 19:10:37 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100399.D Vial: 1
Acq On : 11 Feb 2011 3:23 pm Operator: tdd/cld
Sample : Ippbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 16:19 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 15:44:07 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57)
58)
59)
60)
61)
62)
63)
64)
65)
66)
67)
68)
69)
70)
71)
72)
73)
74)
Ethylbenzene
1,1,1,2 -Tetrachloroethane
m-/p-Xylenes
o-Xylene
Styrene
Bromof orm
1,1,2,2 -Tetrachloroethane
4-Ethyltoluene
2 -Chlorotoluene
1,3, 5-Trimethylbenzene
1,2, 4-Trimethylbenzene
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene
Benzyl chloride
1 , 2 -Dichlorobenzene
1,2, 4-Trichlorobenzene
Hexachlorobutadiene
Naphthalene
18
18
18
19
19
19
19
20
20
20
20
20
20
20
21
22
22
22
.70
.73
.83
.25
.28
.52
.93
.04
. 07
.10
.42
.71
.78
.90
.10
.40
.45
.66
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
157404
84895
246973
132268
113323
141747
98536
231738
164598
192627m
193143
138305
134941
122792
146463
70396
133043
213773
1
1
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
.08
.13
.07
.11
.07
.13
.16
.13
. 01
.12
.15
.20
.16
.12
.24
.34
.78
.47
PPBV
PPBV #
PPBV
PPBV
PPBV #
PPBV #
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
94
88
89
91
91
99
100
97
94
98
99
98
97
98
99
98
98
(Manual Int. "II" (CLD)|
L1100399.D L021111A.M
Fri Feb 11 19:10:38 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100400.D Vial: 1
Acq On : 11 Feb 2011 4:42 pm Operator: tdd/cld
Sample : 2ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:11 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:13:02 2011
Response via : Initial Calibration
TIC: L1100400. D
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
4.00 5.00 6.00 7.00 8.00 9.00 10.001J
L1100400.D L021111A.M Fri Feb 11 19:13:15 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100400.D Vial: 1
Acq On : 11 Feb 2011 4:42 pm Operator: tdd/cld
Sample : 2ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:11 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:10:11 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.43
.23
.60
130
114
117
786578
2307213
1820826
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
10
10
10
11
11
12
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.15
.24
.57
.75
.03
.11
.90
.15
.58
.67
.42
.89
.84
.91
.25
.35
.60
.81
.04
.15
.62
.59
.87
.01
.64
.73
.91
.99
.01
.57
.44
.83
.77
.05
.51
.43
.69
.78
.60
.03
.13
.20
.45
.17
.39
.64
.18
.46
.51
.73
.95
.12
.65
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
30181
263706
235319
38824
56862
53147
142275
26076
139388
339361
19787
13949
165920
85656
99451
139481
87016
19131
27727
60804
72943
144116
22435
51491
81423
134647
66204
18662
16491
164159
19107
219254
55078
261945
131545
156194
94982
31699
140188
40204
39686
47323
186619
85747
127073
215232
83749
106769
181727
109412
307500
226507
267138
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
.42
.13
.11
.13
.21
.30
.10
.07
.14
.25
.43
.33
.32
.24
.35
.16
.34
.24
.45
.11
.25
.26
.22
.33
.22
.29
.20
.31
.38
.20
.33
.19
.31
.25
.26
.10
.22
.25
.19
.17
.25
.19
.19
.25
.33
.17
.02
.17
.17
.34
.32
.12
.18
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.00
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
98
95
99
100
90
100
88
96
99
51
93
100
74
90
92
99
100
77
89
81
99
92
84
97
97
72
97
89
96
96
96
96
99
96
92
99
93
96
96
89
85
98
98
99
96
90
95
96
97
98
100
96
(#) = qualifier out of range (m) =
L1100400.D L021111A.M Fri Feb 11 19:13:13 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100400.D Vial: 1
Acq On : 11 Feb 2011 4:42 pm Operator: tdd/cld
Sample : 2ppbv to!5 std A Inst : Lurch
Misc : Can #1420, 2ppbv to!5 std, SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:11 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:10:11 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.71 91 317456 2.13 PPBV 93
58) 1,1,1,2-Tetrachloroethane 18.74 131 169926 2.22 PPBV # 91
59) m-/p-Xylenes 18.83 91 503168 4.12 PPBV 91
60) o-Xylene 19.25 91 265038 2.19 PPBV 89
61) Styrene 19.28 104 235500 2.16 PPBV # 90
62) Bromoform 19.52 173 287645 2.19 PPBV # 98
63) 1,1,2,2-Tetrachloroethane 19.93 83 197514 2.25 PPBV 99
64) 4-Ethyltoluene 20.05 105 453377 2.12 PPBV 100
65) 2-Chlorotoluene 20.08 91 334534 2.00 PPBV 94
66) 1,3,5-Trimethylbenzene 20.10 105 393566 2.19 PPBV # 77
67) 1,2,4-Trimethylbenzene 20.43 105 384938 2.19 PPBV 100
68) 1,3-Dichlorobenzene 20.71 146 281108 2.27 PPBV 99
69) 1,4-Dichlorobenzene 20.79 146 275014 2.19 PPBV 97
70) Benzyl chloride 20.90 91 257508 2.19 PPBV 96
71) 1,2-Dichlorobenzene 21.10 146 282342 2.23 PPBV 99
72) 1,2,4-Trichlorobenzene 22.41 180 153927 2.33 PPBV 98
73) Hexachlorobutadiene 22.46 225 219405 2.43 PPBV 98
74) Naphthalene 22.66 128 449004 2.46 PPBV 100
L1100400.D L021111A.M
Fri Feb 11 19:13:14 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100386.D Vial: 1
Acq On : 10 Feb 2011 8:50 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:13 2011
Method
Title
Last Update
Response via
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
Quant Results File: L021111A.RES
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100386. D
4.00 5.00 6.00 7.00 8.00 9.00 10.00 1iQO,J2J»J3.0Q UW 15.00 16J)0 17,00.JJ3.00 19.0020.0021.0022.0023.0024.0025.0026.00
L1100386.D L021111A.M Fri Feb 11 19:34:44 2011 Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100386.D Vial: 1
Acq On : 10 Feb 2011 8:50 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:13 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:13:02 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.40
.20
.59
130
114
117
857643
2579573
1951915
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.13
.22
.56
.73
.01
.09
.86
.13
.56
.65
.39
.87
.82
.89
.22
.33
.56
.77
.00
.11
.60
.56
.84
.98
.60
.69
.88
.96
.97
.53
.40
.80
.74
.03
.48
.41
.67
.75
.58
.01
.11
.18
.42
.15
.37
.62
.15
.43
.49
.71
.94
.11
.62
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
74578
639354
577768
93813
131933
121554
347733
61482
351626
797883
45835
32462
381056
208677
232736
351258
219327
49096
69152
147509
176362
348260
55782
121993
191137
336091
164710
45474
38682
407354
45026
534217
131494
621210
320692
401516
236711
79108
354566
102322
98082
120195
463956
222413
318501
552808
215780
273964
444270
277166
753062
570847
671543
5
4
4
4
4
4
4
4
4
4
5
4
4
5
5
5
5
5
3
4
4
5
5
5
4
5
5
5
5
5
5
4
5
4
4
4
4
5
4
4
4
4
4
5
5
5
4
5
4
5
5
4
5
.48
.73
.75
.73
.70
.82
.72
.47
.95
.84
.16
.97
.89
.00
.04
.00
.42
.27
.31
.69
.98
.00
.06
.06
.78
.24
.03
.17
.13
.01
.05
.89
.06
.90
.92
.83
.95
.03
.95
.93
.97
.98
.87
.21
.22
.20
.86
.18
.94
.52
.29
.98
.11
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
-0.02
-0.02
-0.01
Qvalue
# 1
98
# 95
99
99
89
100
100
# 98
99
# 57
95
# 100
# 75
# 91
93
# 99
98
# 85
88
# 80
98
94
90
100
97
# 75
97
# 90
98
91
95
99
99
96
90
99
96
98
100
92
96
98
98
98
98
# 92
91
97
96
98
100
94
(#) = qualifier out of range (m) =
L1100386.D L021111A.M Fri Feb 11 19:34:43 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100386.D Vial: 1
Acq On : 10 Feb 2011 8:50 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:13 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:13:02 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.69 91 806263 5.06 PPBV 93
58) 1,1,1,2-Tetrachloroethane 18.71 131 424929 5.17 PPBV 90
59) m-/p-Xylenes 18.81 91 1290395 9.85 PPBV 90
60) o-Xylene 19.23 91 665689 5.13 PPBV 89
61) Styrene 19.27 104 595122 5.08 PPBV # 90
62) Bromoform 19.51 173 698045 4.96 PPBV # 98
63) 1,1,2,2-Tetrachloroethane 19.92 83 495982 5.27 PPBV 99
64) 4-Ethyltoluene 20.04 105 1160412 5.06 PPBV 99
65) 2-Chlorotoluene 20.07 91 852126 4.75 PPBV 94
66) 1,3,5-Trimethylbenzene 20.09 105 966196 5.01 PPBV # 76
67) 1,2,4-Trimethylbenzene 20.41 105 954867 5.07 PPBV 99
68) 1,3-Dichlorobenzene 20.69 146 684417 5.16 PPBV 99
69) 1,4-Dichlorobenzene 20.77 146 667762 4.96 PPBV 99
70) Benzyl chloride 20.89 91 660308 5.24 PPBV 96
71) 1,2-Dichlorobenzene 21.09 146 681925 5.03 PPBV 99
72) 1,2,4-Trichlorobenzene 22.39 180 302434 4.28 PPBV 98
73) Hexachlorobutadiene 22.44 225 432135 4.46 PPBV 98
74) Naphthalene 22.65 128 877150 4.49 PPBV 99
L1100386.D L021111A.M
Fri Feb 11 19:34:43 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100387.D Vial: 1
Acq On : 10 Feb 2011 9:32 pm Operator: tdd/cld
Sample : lOppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:15 2011
Method
Title
Last Update
Response via
5000000
4800000
Quant Results File: L021111A.RES
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100387. D
4.00 5.00 6.00 7.00 8.00 9.00 10.001J
L1100387.D L021111A.M Fri Feb 11 19:35:05 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100387.D Vial: 1
Acq On : 10 Feb 2011 9:32 pm Operator: tdd/cld
Sample : lOppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:15 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:15:20 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.40
.20
.60
130
114
117
839895
2550701
1906841
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.14
.23
.55
.73
.01
.09
.87
.13
.55
.65
.40
.86
.82
.89
.21
.32
.56
.78
.00
.12
.59
.55
.84
.98
.61
.69
.88
.95
.97
.54
.41
.79
.74
.03
.48
.40
.67
.75
.57
.00
.10
.17
.42
.14
.36
.62
.15
.44
.49
.71
.93
.10
.63
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
144461
1294838
1171236
191715
268229
247335
679520
121958
696507
1544427
85881
65110
738602
409288
440278
678677
403713
94243
121374
283900
344459
677892
113797
239218
388259
676172
325151
90841
74340
804970
91835
1049016
256245
1208040
643306
786963
471019
157765
703635
204995
198378
239664
949538
450149
629818
1113997
454904
554118
909728
598516
1533709
1187353
1380622
10
9
9
9
9
10
9
9
10
9
9
10
9
10
9
9
10
10
5
9
9
9
10
10
9
10
10
10
10
10
10
9
10
9
9
9
9
10
9
9
10
10
10
10
10
10
10
10
10
12
11
10
10
.84
.78
.83
.87
.76
.02
.41
.05
.01
.57
.88
.19
.68
.02
.74
.87
.18
.33
.93
.22
.94
.95
.54
.13
.91
.76
.14
.56
.07
.10
.51
.82
.07
.73
.99
.57
.96
.14
.93
.99
.16
.04
.08
.66
.44
.73
.48
.73
.35
.21
.03
.60
.75
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.00
Qvalue
# 1
99
# 95
98
98
89
99
97
# 98
100
# 60
93
# 100
# 75
# 90
93
# 100
98
# 83
89
# 79
99
92
89
99
96
# 73
95
94
99
96
96
98
100
95
90
99
94
97
97
89
92
99
99
99
98
# 92
92
96
# 94
98
100
93
(#) = qualifier out of range (m) =
L1100387.D L021111A.M Fri Feb 11 19:35:04 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100387.D Vial: 1
Acq On : 10 Feb 2011 9:32 pm Operator: tdd/cld
Sample : lOppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 250mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:15 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:15:20 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.69 91 1661001 10.66 PPBV 93
58) 1,1,1,2-Tetrachloroethane 18.72 131 851994 10.62 PPBV 90
59) m-/p-Xylenes 18.82 91 2675935 20.92 PPBV 90
60) o-Xylene 19.23 91 1374559 10.85 PPBV 89
61) Styrene 19.26 104 1242717 10.86 PPBV # 90
62) Bromoform 19.50 173 1436563 10.44 PPBV # 99
63) 1,1,2,2-Tetrachloroethane 19.92 83 995481 10.83 PPBV 100
64) 4-Ethyltoluene 20.03 105 2451421 10.94 PPBV 99
65) 2-Chlorotoluene 20.06 91 1735073 9.91 PPBV 94
66) 1,3,5-Trimethylbenzene 20.08 105 1932307 10.27 PPBV # 77
67) 1,2,4-Trimethylbenzene 20.41 105 1963897 10.68 PPBV 100
68) 1,3-Dichlorobenzene 20.70 146 1418418 10.95 PPBV 99
69) 1,4-Dichlorobenzene 20.77 146 1399159 10.64 PPBV 99
70) Benzyl chloride 20.88 91 1392805 11.32 PPBV 97
71) 1,2-Dichlorobenzene 21.09 146 1376121 10.38 PPBV 99
72) 1,2,4-Trichlorobenzene 22.39 180 754475 10.93 PPBV 98
73) Hexachlorobutadiene 22.45 225 864172 9.14 PPBV 98
74) Naphthalene 22.64 128 2065957 10.82 PPBV 99
L1100387.D L021111A.M
Fri Feb 11 19:35:04 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100388.D Vial: 1
Acq On : 10 Feb 2011 10:19 pm Operator: tdd/cld
Sample : 20ppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:17 2011
Method
Title
Last Update
Response via
1.3e+07
Quant Results File: L021111A.RES
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100388. D
4.00 5.00 6.00 7.00 8.00 9.00 10.001J
L1100388.D L021111A.M Fri Feb 11 19:35:23 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100388.D Vial: 1
Acq On : 10 Feb 2011 10:19 pm Operator: tdd/cld
Sample : 20ppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:17 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:17:25 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.40
.20
.59
130
114
117
835157
2566607
2123122
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.13
.22
.55
.73
.01
.09
.87
.13
.56
.65
.40
.86
.82
.89
.22
.33
.57
.78
.00
.12
.60
.56
.84
.99
.61
.69
.88
.96
.98
.53
.41
.80
.74
.04
.48
.41
.67
.75
.58
.01
.11
.17
.42
.15
.37
.62
.15
.43
.49
.70
.94
.11
.62
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
297583
2735999
2490606
396774
565920
522994
1477730
262992
1468999
3181056
149744
134015
1582971
879351
949573
1464471
860082
204590
243609
596465
738552
1467247
245147
522180
820169
1448966
699189
199984
156757
1731903
195271
2233549
539802
2539972
1382099
1674600
1021984
332781
1504650
442016
433243
523277
2076147
1003301
1349835
2494579
995591
1234939
1984464
1359875
3315590
2659358
3121132
22
20
21
20
20
21
20
19
21
19
17
21
20
21
21
21
21
22
11
19
21
21
22
22
21
23
21
23
21
21
22
21
21
20
21
20
21
21
21
21
22
21
21
23
22
21
20
21
20
24
21
21
21
.46
.78
.03
.54
.72
.30
.59
.62
.22
.83
.33
.10
.86
.65
.12
.41
.82
.54
.97
.47
.43
.65
.83
.24
.05
.19
.92
.37
.36
.86
.48
.02
.34
.57
.32
.24
.47
.27
.09
.41
.04
.78
.91
.62
.24
.58
.61
.48
.28
.92
.42
.32
.83
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.00
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
99
95
99
100
87
100
100
98
100
60
95
100
72
90
92
99
96
81
89
78
99
92
88
99
96
72
89
90
99
96
96
96
99
96
90
99
93
96
98
91
92
99
99
98
98
91
91
95
95
98
100
92
(#) = qualifier out of range (m) =
L1100388.D L021111A.M Fri Feb 11 19:35:22 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100388.D Vial: 1
Acq On : 10 Feb 2011 10:19 pm Operator: tdd/cld
Sample : 20ppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", SOOmL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:17 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:17:25 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.69 91 3784992 21.82 PPBV 93
58) 1,1,1,2-Tetrachloroethane 18.71 131 1840938 20.61 PPBV # 89
59) m-/p-Xylenes 18.81 91 6196219 43.50 PPBV 90
60) o-Xylene 19.23 91 3103120 21.99 PPBV 89
61) Styrene 19.25 104 2837590 22.28 PPBV # 89
62) Bromoform 19.51 173 3146491 20.54 PPBV # 99
63) 1,1,2,2-Tetrachloroethane 19.92 83 2193013 21.42 PPBV 100
64) 4-Ethyltoluene 20.03 105 5570554 22.34 PPBV 98
65) 2-Chlorotoluene 20.06 91 3939741 20.20 PPBV 96
66) 1,3,5-Trimethylbenzene 20.08 105 4364478 20.82 PPBV # 78
67) 1,2,4-Trimethylbenzene 20.40 105 4349938 21.24 PPBV 99
68) 1,3-Dichlorobenzene 20.69 146 3180292 22.05 PPBV 100
69) 1,4-Dichlorobenzene 20.76 146 3119122 21.31 PPBV 100
70) Benzyl chloride 20.87 91 3215715 23.48 PPBV 97
71) 1,2-Dichlorobenzene 21.09 146 3036898 20.58 PPBV 100
72) 1,2,4-Trichlorobenzene 22.38 180 1919479 24.97 PPBV 98
73) Hexachlorobutadiene 22.43 225 1961742 18.63 PPBV 98
74) Naphthalene 22.64 128 5158679 24.27 PPBV 100
L1100388.D L021111A.M
Fri Feb 11 19:35:22 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100389.D Vial: 1
Acq On : 10 Feb 2011 11:10 pm Operator: tdd/cld
Sample : 30ppbv to-15 std A Inst : Lurch
Can #1464 "A", 750mL Multiplr: 1.00
Misc
MS Integration Params: rteint.p
Quant Time: Feb 11 19:19 2011
Quant Results File: L021111A.RES
Method
Title
Last Update
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
2e+07
TIC: L1100389. D
1.9e+07
1.8e+07
1.76+07
1.66+07
1.56+07
1.46+07
1.36+07
1.26+07
1.16+07
1e+07
9000000
8000000
7000000
6000000
5000000
4000000
3000000
2000000
1000000
J-i—r
4.00 5.00 6.00 7.00 8.00 9.00 10.001J
L1100389.D L021111A.M Fri Feb 11 19:35:39 2011
.00 19.00 20.00 21.00 22.00 23.00 24.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100389.D Vial: 1
Acq On : 10 Feb 2011 11:10 pm Operator: tdd/cld
Sample : 30ppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 750mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:19 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:19:41 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.41
.21
.59
130
114
117
981377
3055944
2445621
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
9
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.14
.23
.56
.73
.01
.09
.87
.13
.56
.65
.40
.87
.82
.89
.22
.33
.57
.77
.01
.13
.60
.55
.84
.99
.61
.70
.88
.95
.97
.54
.41
.80
.75
.03
.48
.41
.66
.76
.57
.01
.10
.17
.43
.14
.36
.62
.15
.44
.49
.71
.94
.10
.62
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
441898
4243083
3879623
596495
864970
790240
2340960
413458
2273225
4775749
271983
214091
2449299
1384571
1482481
2334534
1360751
324964
376404
929060
1180762
2374473
379715
824425
1293675
2291824
1115219
323504
246734
2785472
313075
3509049
859404
3926940
2225237
2677149
1620141
526925
2369852
685694
694312
835482
3294550
1605454
2099993
4040502
1602792
1986460
3123027
2134044
5133499
4274708
5026949
28
27
27
26
26
27
27
26
27
25
26
28
27
29
28
29
29
30
15
25
29
29
30
29
28
31
29
32
28
29
30
28
28
27
28
27
28
28
27
27
29
29
29
31
29
30
28
30
27
33
28
29
30
.39
.42
.87
.27
.95
.39
.76
.26
.95
.34
.78
.68
.47
.01
.06
.04
.38
.47
.73
.81
.15
.81
.09
.88
.25
.21
.76
.17
.61
.92
.67
.10
.92
.06
.83
.17
.59
.28
.90
.89
.67
.20
.20
.75
.05
.35
.80
.00
.70
.95
.79
.75
.52
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.00
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
99
95
100
100
86
99
99
98
99
61
90
100
70
90
91
100
95
83
85
77
98
94
88
100
95
70
83
92
99
98
96
94
99
96
90
99
90
95
96
89
93
99
99
96
98
91
90
95
94
98
100
92
(#) = qualifier out of range (m) =
L1100389.D L021111A.M Fri Feb 11 19:35:38 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100389.D Vial: 1
Acq On : 10 Feb 2011 11:10 pm Operator: tdd/cld
Sample : 30ppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 750mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 11 19:19 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:19:41 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57)
58)
59)
60)
61)
62)
63)
64)
65)
66)
67)
68)
69)
70)
71)
72)
73)
74)
Ethylbenzene
1,1,1,2 -Tetrachloroethane
m-/p-Xylenes
o-Xylene
Styrene
Bromof orm
1,1,2,2 -Tetrachloroethane
4-Ethyltoluene
2 -Chlorotoluene
1,3, 5-Trimethylbenzene
1,2, 4-Trimethylbenzene
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene
Benzyl chloride
1 , 2 -Dichlorobenzene
1,2, 4-Trichlorobenzene
Hexachlorobutadiene
Naphthalene
18
18
18
19
19
19
19
20
20
20
20
20
20
20
21
22
22
22
.69
.72
.82
.23
.26
.50
.92
.03
.06
.08
.41
.69
.77
.88
.08
.39
.44
.64
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
6145708
2841255
10113083
5050471
4724566
4926186
3503985
9008661
6456394
6881840
7095395
4975003
4914828
5333436
4736849
3384421
3097432
8910079
30.
27.
61.
31.
32.
27.
29.
31.
28.
28.
30.
29.
29.
33.
27.
38.
25.
36.
76
61
63
07
20
91
72
36
74
51
08
94
15
80
87
21
54
39
PPBV
PPBV #
PPBV
PPBV
PPBV #
PPBV #
PPBV
PPBV
PPBV
PPBV #
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
94
87
89
89
88
98
100
99
98
79
98
99
99
98
100
99
98
100
L1100389.D L021111A.M
Fri Feb 11 19:35:38 2011
Page 2
-------
Quantitation Report
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021111A\L1100407.D Vial: 1
Acq On : 11 Feb 2011 9:43 pm Operator: tdd/cld
Sample : Sppbv ICV/LCS std B Inst : Lurch
Misc : Can #1449 "B", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 14 6:50 2011 Quant Results File: L021111A.RES
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
Last Update : Fri Feb 11 19:26:13 2011
Response via : Initial Calibration
TIC: L1100407. D
4000000
3800000
3600000
3400000
3200000
3000000
2800000
2600000
2400000
2200000
2000000
1800000
1600000
1400000
o
CM
O
JJU
1200000
1000000
800000
600000
400000
200000
4.00 5.00 6.00 7.00 8.00 9.00 10.001.
L1100407.D L021111A.M Mon Feb 14 10:18:51 2011
00 19.00 20.00 21.00 22.00 23.00 24.00 25.0026.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021111A\L1100407.D Vial: 1
Acq On : 11 Feb 2011 9:43 pm Operator: tdd/cld
Sample : Sppbv ICV/LCS std B Inst : Lurch
Misc : Can #1449 "B", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 14 6:50 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.42
.22
.60
130
114
117
865954
2602128
1957945
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
10
10
10
11
11
12
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.15
.24
.57
.74
.02
.10
.88
.15
.57
.66
.41
.88
.83
.90
.23
.34
.59
.80
.02
.14
.61
.57
.86
.00
.63
.71
.90
.98
.00
.55
.43
.81
.76
.04
.49
.41
.68
.77
.58
.02
.12
.19
.44
.15
.37
.63
.16
.45
.50
.72
.94
.11
.64
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
72263
641518
585561
94607
134044
125460
342038
61312
333752
785912
47947
28072
364493
203178
230223
340681
228401
46832
74465
144484
171949
351222
58001
117966
190149
310945
157758
47508
39416
401884
47016
516818
124439
599590
314238
388071
230630
75630
338588
99602
97535
128867
457516
215689
348639
530347
212055
263649
426374
321170
740131
564549
650744
5
4
4
4
4
4
4
4
4
4
5
4
4
4
4
4
5
4
4
4
4
5
5
4
4
4
4
5
5
4
5
4
4
4
4
4
4
4
4
4
4
5
4
5
5
4
4
4
4
6
5
4
4
.26
.70
.77
.72
.73
.93
.60
.41
.65
.73
.35
.26
.63
.82
.94
.80
.59
.98
.97
.55
.81
.00
.21
.85
.71
.80
.77
.35
.18
.89
.22
.69
.74
.68
.78
.63
.78
.77
.68
.76
.89
.29
.76
.01
.66
.98
.76
.97
.72
.38
.18
.91
.93
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.01
0.01
0.00
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
98
95
99
99
92
100
93
97
100
61
87
100
73
90
94
100
96
83
90
80
99
96
91
99
97
72
95
88
99
94
96
97
99
95
91
99
90
98
99
92
96
98
97
99
96
91
94
96
97
97
99
93
(#) = qualifier out of range (m) =
L1100407.D L021111A.M Mon Feb 14 10:18:50 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021111A\L1100407.D Vial: 1
Acq On : 11 Feb 2011 9:43 pm Operator: tdd/cld
Sample : Sppbv ICV/LCS std B Inst : Lurch
Misc : Can #1449 "B", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 14 6:50 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57)
58)
59)
60)
61)
62)
63)
64)
65)
66)
67)
68)
69)
70)
71)
72)
73)
74)
Ethylbenzene
1,1,1,2 -Tetrachloroethane
m-/p-Xylenes
o-Xylene
Styrene
Bromof orm
1,1,2,2 -Tetrachloroethane
4-Ethyltoluene
2 -Chlorotoluene
1,3, 5-Trimethylbenzene
1,2, 4-Trimethylbenzene
1 , 3 -Dichlorobenzene
1, 4 -Dichlorobenzene
Benzyl chloride
1 , 2 -Dichlorobenzene
1,2, 4-Trichlorobenzene
Hexachlorobutadiene
Naphthalene
18
18
18
19
19
19
19
20
20
20
20
20
20
20
21
22
22
22
.70
.73
.82
.24
.27
.51
.92
.03
.07
.09
.41
.70
.77
.88
.09
.39
.44
.65
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
794602
417405
1290751
660694
590785
692762
497706
1224996
881154
1013359
1004976
694267
675098
639495
695252
339162
448249
973585
4.
5.
9.
5.
5.
4.
5.
5.
4.
5.
5.
5.
5.
5.
5.
4.
4.
4.
97
07
83
08
03
90
27
33
90
24
32
22
00
06
11
78
62
97
PPBV
PPBV
PPBV
PPBV
PPBV #
PPBV #
PPBV
PPBV
PPBV
PPBV #
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
94
90
91
88
91
99
100
99
95
77
99
99
98
97
99
100
98
99
L1100407.D L021111A.M
Mon Feb 14 10:18:50 2011
Page 2
-------
Company In-House
Analyst CLD
Parameters EPA Method TO-15
# Samples
Sample ID: Sppbv ICV/LCS std
Data File: L1100407.D
B
Client #
Job#
PO#
Report Date
ICV for Curve L021111A
NA
NA
2/14/2011
DF: 1.00
TankDF: 1.00
Tank/Misc ID: Can #1449 "B", 125mL
Compound
ropylene
reon 12 (CCI2F2)
reon114(C2CI2F4)
hloromethane
hloroethene (Vinyl chloride)
3-Butadiene
romomethane
hloroethane
romoethene (Vinyl bromide)
reon 1 1 (CCI3F)
thanol
croleln
reon113(C2CI3F3)
1-Dlchloroethene
cetone
arbon dlsulflde
opropyl alcohol
llyl chloride (3-chloropropene)
cetonltrlle
ethylene chloride
ans-1 ,2-Dlchloroethene
ethyl tert-butyl ether
crylonltrlle
exane
1-Dlchloroethane
Inyl acetate
s-1 ,2-Dichloroethylene
ethyl ethyl ketone (2-Butanone)
thyl acetate
hloroform
etrahydrofuran
1,1-Trichloroethane
yclohexane
arbon tetrachlorlde
enzene
2,4-trlmethylpentane
2-Dlchloroethane
eptane
rlchloroethene
2-Dichloropropane
ethyl methacrylate
4-Dloxane
romodlchloromethane
s-1 ,3-Dlchloropropene
ethyl Isobutyl ketone
oluene
ans-1 ,3-Dlchloropropene
1 ,2-Trichloroethane
etrachloroethene
-Hexanone (Methyl butyl ketone)
Quant
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
Ret.
Time
(min)
4.15
4.24
4.57
4.74
5.02
5.10
5.88
6.15
6.57
6.66
7.41
7.88
7.83
7.90
8.23
8.34
8.59
8.80
9.02
9.14
9.61
9.57
9.86
10.00
10.63
10.71
11.90
11.98
12.00
12.55
12.43
12.81
12.76
13.04
13.50
13.41
13.68
13.77
14.58
15.02
15.12
15.19
15.44
16.15
16.37
16.63
17.16
17.45
17.50
17.72
ConCa
1
Cone, as Sample
Area
72,263
641,518
585,561
94,607
134,044
125,460
342,038
61,312
333,752
785,912
47,947
28,072
364,493
203,178
230,223
340,681
228,401
46,832
74,465
144,484
171,949
351,222
58,001
117,966
190,149
310,945
157,758
47,508
39,416
401,884
47,016
516,818
124,439
599,590
314,238
388,071
230,630
75,630
338,588
99,602
97,535
128,867
457,516
215,689
348,639
530,347
212,055
263,649
426,374
321,170
Analyzed Cone. Flags Tag Value
(ppbv) (ppbv)
5.26 5.26
4.70 4.70
4.77 4.77
4.72 4.72
4.73 4.73
4.93 4.93
4.60 4.60
4.41 4.41
4.65 4.65
4.73 4.73
5.35 5.35
4.26 4.26
4.63 4.63
4.82 4.82
4.94 4.94
4.80 4.80
5.59 5.59
4.98 4.98
4.97 4.97
4.55 4.55
4.81 4.81
5.00 5.00
5.21 5.21
4.85 4.85
4.71 4.71
4.80 4.80
4.77 4.77
5.35 5.35
5.18 5.18
4.89 4.89
5.22 5.22
4.69 4.69
4.74 4.74
4.68 4.68
4.78 4.78
4.63 4.63
4.78 4.78
4.77 4.77
4.68 4.68
4.76 4.76
4.89 4.89
5.29 5.29
4.76 4.76
5.01 5.01
5.66 5.66
4.98 4.98
4.76 4.76
4.97 4.97
4.72 4.72
6.38 6.38
5.50
4.90
4.90
4.95
4.90
5.15
4.90
4.85
4.95
5.05
5.30
5.25
5.15
5.20
5.30
5.10
5.50
5.15
4.55
5.20
5.15
5.20
5.15
5.37
5.10
5.25
5.15
5.30
5.10
5.10
5.35
5.05
5.15
5.10
5.10
4.95
5.10
5.20
5.10
5.15
5.00
5.10
5.10
5.45
5.05
5.15
4.85
5.15
5.05
5.35
%Diff
Uttt.
From Tag
4.3
4.1
2.7
4.6
3.4
4.3
6.2
9.0
6.0
6.4
1.0
18.8
10.0
7.2
6.8
5.8
1.6
3.4
9.1
12.5
6.6
3.9
1.1
9.8
7.7
8.6
7.4
1.0
1.6
4.1
2.4
7.1
7.9
8.2
6.3
6.5
6.3
8.3
8.2
7.6
2.1
3.7
6.6
8.1
12.2
3.4
1.9
3.4
6.5
19.3
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
2/14/2011
-------
Company In-House
Analyst CLD
Parameters EPA Method TO-15
# Samples
Sample ID: Sppbv ICV/LCS std
Data File: L1100407.D
B
Client #
Job#
PO#
Report Date
ICV for Curve L021111A
NA
NA
2/14/2011
DF: 1.00
TankDF: 1.00
Tank/Misc ID: Can #1449 "B", 125mL
Compound
Ibromochloromethane
2-Dibromoethane
hlorobenzene
thylbenzene
1 , 1 ,2-Tetrachloroethane
i-/p-Xylenes
-Xylene
tyrene
romoform
1 ,2,2-Tetrachloroethane
-Ethyltoluene
-Chlorotoluene
3,5-Trimethylbenzene
2,4-Trimethylbenzene
3-Dlchlorobenzene
4-Dlchlorobenzene
enzyl chloride
2-Dlchlorobenzene
2,4-Trichlorobenzene
exachlorobutadlene
aphthalene
Quant
129
107
112
91
131
91
91
104
173
83
105
91
105
105
146
146
91
146
180
225
128
Ret.
Time
(min)
17.94
18.11
18.64
18.70
18.73
18.82
19.24
19.27
19.51
19.92
20.03
20.07
20.09
20.41
20.70
20.77
20.88
21.09
22.39
22.44
22.65
ConCa
1
Cone, as Sample
Area
740,131
564,549
650,744
794,602
417,405
1,290,751
660,694
590,785
692,762
497,706
1,224,996
881,154
1,013,359
1,004,976
694,267
675,098
639,495
695,252
339,162
448,249
973,585
Analyzed Cone. Flags Tag Value
(ppbv) (ppbv)
5.18 5.18
4.91 4.91
4.93 4.93
4.97 4.97
5.07 5.07
9.83 9.83
5.08 5.08
5.03 5.03
4.90 4.90
5.27 5.27
5.33 5.33
4.90 4.90
5.24 5.24
5.32 5.32
5.22 5.22
5.00 5.00
5.06 5.06
5.11 5.11
4.78 4.78
4.62 4.62
4.97 4.97
5.35
5.10
5.25
5.20
5.20
10.2
5.25
5.20
5.10
5.25
5.15
4.95
5.15
5.15
5.20
5.05
5.20
5.10
4.95
5.10
5.21
%niff
Ulll.
From Tag
3.1
3.8
6.0
4.5
2.6
3.7
3.3
3.3
3.9
0.4
3.4
1.0
1.8
3.3
0.4
1.0
2.6
0.2
3.4
9.5
4.6
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
Rec. (%) Flags
romochloromethane (IS)
4-Dlfluorobenzene (IS)
hlorobenzene-d5 (IS)
130
114
117
12.42
14.22
18.60
865,954
2,602,128
1,957,945
11.24 N/A
11.93 N/A
11.13 N/A
Internal Standard Acceptance Criteria (Area +/- 40%. RT +/- 20 sec.l
Internal Standard
Name
Bromochloromethane (IS)
1 ,4-Dlfluorobenzene (IS)
Chlorobenzene-d5 (IS)
Concal
RT
12.42
14.22
18.61
Sample
RT
12.42
14.22
18.60
Ave. ICal
IS Area
848,241
2,554,861
1,992,369
Sample
IS Area
865,954
2,602,128
1,957,945
Flag
Pass
Pass
Pass
2/14/2011
-------
Evaluate Continuing Calibration Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100435.D Vial: 1
Acq On : 17 Feb 2011 3:27 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Method
Title
Last Update
Response via
Min. RRF
Max. RRF Dev
Compound
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Multiple Level Calibration
0.000 Min. Rel. Area
30% Max. Rel. Area
AvgRF
40% Max. R.T. Dev O.SOmin
200%
CCRF
%Dev Area% Dev(min)
1 I
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36 I
37
38
39
40
41
42
43
44
45
46
47
48 I
49
50
51
52
53
54
55
Bromochloromethane (IS)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlorid
1, 3 -Butadiene
B r omome thane
Chloroethane
Bromoethene (Vinyl bromide)
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropro
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2-Buta
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
1, 4-Dif luorobenzene (IS)
Benzene
2,2, 4-trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Chlorobenzene-d5 (IS)
Toluene
trans- 1, 3-Dichloropropene
1,1,2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl ke
Dibromochloromethane
1 , 2 -Dibromoethane
1
0
1
1
0
0
0
0
0
0
2
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
1
0
1
1
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
.000
.178
.772
.594
.260
.368
.330
.966
.180
.932
.159
.116
.085
.021
.547
.605
.921
.530
.122
.195
.412
.464
.912
.145
.316
.524
.841
.429
.115
.099
.066
.117
.430
.340
.662
.000
.301
.385
.221
.073
.332
.096
.091
.112
.440
.197
.282
.000
.606
.253
.301
.513
.286
.812
.654
1
0
1
1
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
1
1
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
.000
.131
.574
.364
.195
.283
.249
.795
.134
.819
.970
.093
.071
.988
.475
.502
.765
.446
.106
.155
.322
.407
.819
.119
.264
.454
.703
.377
.098
.082
.952
.100
.319
.287
.576
.000
.276
.337
.209
.062
.328
.083
.080
.103
.402
.184
.244
.000
.552
.231
.270
.484
.245
.757
.577
0
26
11
14
25
23
24
17
25
12
8
19
16
3
13
17
16
15
13
20
21
12
10
17
16
13
16
12
14
17
10
14
7
15
5
0
8
12
5
15
1
13
12
8
8
6
13
0
8
8
10
5
14
6
11
.0
.4
.2
.4
.0
.1
.5
.7
.6
.1
.8
.8
.5
.2
.2
.0
.9
.8
.1
.5
.8
.3
.2
.9
.5
.4
.4
.1
.8
.2
.7
.5
.8
.6
.2
.0
.3
.5
.4
.1
.2
.5
.1
.0
.6
.6
.5
.0
.9
.7
.3
.7
.3
.8
.8
127
94
117
112
100
102
102
110
103
115
121
106
110
129
114
111
110
109
111
101
110
115
119
104
111
117
107
114
110
106
118
115
122
109
125
124
118
110
121
109
128
112
110
118
120
117
108
131
119
119
118
128
110
125
118
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
.00
.00
.00
.00
.02
.00
.00
.02
.02
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.02
.00
.00
.02
.00
.00
.02
.00
.00
.02
.00
.00
.00
.00
.00
.00
.02
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
.00
(#) = Out of Range
L1100435.D L021111A.M
"EA#" 02"lT-49" "Page"89 off 06"
Fri Feb 18 13:10:17 2011
Page 1
-------
Evaluate Continuing Calibration Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100435.D Vial: 1
Acq On : 17 Feb 2011 3:27 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Method
Title
Last Update
Response via
Min. RRF
Max. RRF Dev
Compound
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Multiple Level Calibration
0.000 Min. Rel. Area
30% Max. Rel. Area
AvgRF
40% Max. R.T. Dev O.SOmin
200%
CCRF
%Dev Area% Dev(min)
Chlorobenzene
Ethylbenzene
1,1,1,2-Tetrachloroethane
m-/p-Xylenes
o-Xylene
Styrene
Bromoform
1,1,2,2-Tetrachloroethane
4-Ethyltoluene
2-Chlorotoluene
1,3,5-Trimethylbenzene
1,2,4-Trimethylbenzene
1, 3-Dichlorobenzene
1,4-Dichlorobenzene
Benzyl chloride
1,2-Dichlorobenzene
1,2,4-Trichlorobenzene
Hexachlorobutadiene
Naphthalene
0.750
0.909
0.468
0.747
0.740
0.668
0.803
0.537
1.307
.022
.099
.074
0.756
0.767
0.718
0.774
0.403
0.552
1.114
1.
1.
1.
0.680
0.811
0.438
0.654
0.664
0.602
0.746
0.448
1.127
0.892
0.982
0.940
0.707
0.709
0.629
0.719
0.338
0.481
0.898
9.3
10.8
6.4
12.4
10.3
9.9
7.1
16.6
13.8
12.7
10.6
12.5
6.5
7.6
12.
7.
16.
12.9
19.4
.4
.1
.1
122
120
123
119
121
121
125
110
116
117
120
118
125
124
115
125
131
132
124
0.00
0.00
0.00
0.00
0.02
0.02
0.02
0.00
0.02
0.02
0.02
0.02
0.03
0.03
0.03
0.03
0.03
0.03
0.04
(#) = Out of Range
L1100435.D L021111A.M
SPCC '
Fri Feb 18 13:10:18 2011
Page 2
-------
Quantitation Report
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100435.D Vial: 1
Acq On : 17 Feb 2011 3:27 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 16:39 2011
Method
Title
Last Update
Response via
6200000i
Quant Results File: L021111A.RES
M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
TIC: L1100435. D
4.00 5.00 6.00 7.00 8.00 9.00 10.001J
L1100435.D L021111A.M Fri Feb 18 13:09:56 2011
00 19.00 20.00 21.00 22.00 23.0024.00 25.00 26.00
Page 3
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100435.D Vial: 1
Acq On : 17 Feb 2011 3:27 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 16:39 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Internal Standards
R.T. Qlon Response Cone Units Dev(Min)
1)
36)
48)
Bromochloromethane (IS)
1, 4-Dif luorobenzene (IS)
Chlorobenzene-d5 (IS)
12
14
18
.41
.20
.60
130
114
117
1089684
3198975
2556498
11
11
11
.24
.93
.13
PPBV
PPBV
PPBV
Target Compounds
2)
3)
4)
5)
6)
7)
8)
9)
10)
11)
12)
13)
14)
15)
16)
17)
18)
19)
20)
21)
22)
23)
24)
25)
26)
27)
28)
29)
30)
3D
32)
33)
34)
35)
37)
38)
39)
40)
41)
42)
43)
44)
45)
46)
47)
49)
50)
51)
52)
53)
54)
55)
56)
Propylene
Freon 12 (CC12F2)
Freon 114 (C2C12F4)
Chloromethane
Chloroethene (Vinyl chlori
1, 3 -Butadiene
Bromomethane
Chloroethane
Bromoethene (Vinyl bromide
Freon 11 (CC13F)
Ethanol
Acrolein
Freon 113 (C2C13F3)
1, 1-Dichloroethene
Acetone
Carbon disulfide
Isopropyl alcohol
Allyl chloride (3-chloropr
Acetonitrile
Methylene chloride
trans- 1, 2-Dichloroethene
Methyl tert -butyl ether
Acrylonitrile
Hexane
1, 1-Dichloroethane
Vinyl acetate
cis-1, 2-Dichloroethylene
Methyl ethyl ketone (2 -But
Ethyl acetate
Chloroform
Tetrahydrofuran
1,1, 1-Trichloroethane
Cyclohexane
Carbon tetrachloride
Benzene
2,2,4 - trimethylpentane
1 , 2 -Dichloroethane
Heptane
Trichloroethene
1 , 2 -Dichloropropane
Methyl methacrylate
1, 4-Dioxane
Bromodichloromethane
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene
trans- 1, 3-Dichloropropene
1,1, 2 -Trichloroethane
Tetrachloroethene
2-Hexanone (Methyl butyl k
Dibromochloromethane
1 , 2 -Dibromoethane
Chlorobenzene
4
4
4
4
5
5
5
6
6
6
7
7
7
7
8
8
8
8
9
9
9
9
9
10
10
10
11
11
11
12
12
12
12
13
13
13
13
13
14
15
15
15
15
16
16
16
17
17
17
17
17
18
18
.14
.23
.57
.75
.02
.10
.88
.15
.57
.66
.40
.88
.83
.90
.23
.34
.58
.78
.01
.13
.61
.57
.85
.00
.62
.70
.89
.97
.98
.54
.42
.81
.76
.04
.49
.41
.68
.75
.58
.02
.11
.18
.43
.15
.37
.63
.15
.44
.49
.72
.94
.11
.63
41
85
85
50
62
39
94
64
106
101
45
56
151
61
43
76
45
76
41
49
61
73
53
57
63
43
61
72
45
83
72
97
56
117
78
57
62
57
130
63
69
88
83
75
43
91
75
97
166
43
129
107
112
70075
747554
647953
93491
134552
124371
383628
63617
404797
964601
48439
35616
493434
237342
257955
385622
237981
54419
69821
162257
203222
412930
58284
135567
224493
357968
188143
50115
41151
479899
51994
652203
143182
779395
377540
442627
286389
86221
452300
114538
108357
141924
554826
260912
343025
659293
256948
323021
566685
303711
939020
676414
820053
4
4
4
3
3
3
4
3
4
4
4
4
4
4
4
4
4
4
3
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
5
4
4
4
4
4
4
4
4
4
4
4
5
4
4
.05
.35
.19
.71
.78
.88
.10
.64
.48
.61
.30
.30
.98
.48
.40
.32
.63
.60
.70
.06
.52
.67
.16
.42
.42
.39
.52
.49
.30
.64
.59
.70
.34
.84
.67
.29
.83
.42
.09
.45
.42
.74
.70
.93
.53
.74
.42
.67
.81
.62
.04
.50
.76
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
0.00
0.00
0.00
Qvalue
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
#
1
99
93
99
99
89
99
98
98
100
58
93
100
70
90
94
99
93
87
88
77
98
97
91
98
94
67
94
92
99
98
95
93
100
96
92
99
89
98
97
89
92
98
100
98
100
90
91
96
95
99
100
93
(#) = qualifier out of range (m) =
L1100435.D L021111A.M Fri Feb 18 13:09:55 2011
Page 1
-------
Quantitation Report
(QT Reviewed)
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100435.D Vial: 1
Acq On : 17 Feb 2011 3:27 pm Operator: tdd/cld
Sample : Sppbv to-15 std A Inst : Lurch
Misc : Can #1464 "A", 125mL Multiplr: 1.00
MS Integration Params: rteint.p
Quant Time: Feb 17 16:39 2011
Quant Results File: L021111A.RES
Quant Method
Title
Last Update
Response via
DataAcq Meth
M:\MS2011\M...\L021111A.M (RTE Integrator)
TO15 - 72 TCL - Primary "A"
Fri Feb 11 19:26:13 2011
Initial Calibration
MM624-L
Compound
R.T. Qlon Response Cone Unit Qvalue
57) Ethylbenzene 18.69 91 968967
58) 1,1,1,2-Tetrachloroethane 18.72 131 523528
59) m-/p-Xylenes 18.82 91 1532698
60) o-Xylene 19.25 91 808185
61) Styrene 19.28 104 719207
62) Bromoform 19.52 173 873646
63) 1,1,2,2-Tetrachloroethane 19.93 83 544884
64) 4-Ethyltoluene 20.05 105 1346645
65) 2-Chlorotoluene 20.08 91 993996
66) 1,3,5-Trimethylbenzene 20.10 105 1161808m
67) 1,2,4-Trimethylbenzene 20.42 105 1122908
68) 1,3-Dichlorobenzene 20.71 146 852279
69) 1,4-Dichlorobenzene 20.80 146 830531
70) Benzyl chloride 20.91 91 758135
71) 1,2-Dichlorobenzene 21.11 146 850502
72) 1,2,4-Trichlorobenzene 22.41 180 395778
73) Hexachlorobutadiene 22.47 225 568651
74) Naphthalene 22.68 128 1083407
4.
4.
.64
.87
8.94
4.76
4.69
4.74
4.42
4.48
4.23
4.60
4.55
4.91
4.71
4.60
4.79
4.28
4.49
4.23
PPBV
PPBV
PPBV
PPBV
PPBV #
PPBV #
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
PPBV
93
91
90
91
90
98
99
99
Int. "WP" (TDDJ
99
99
95
98
99
98
99
L1100435.D L021111A.M
Fri Feb 18 13:09:55 2011
Page 2
-------
BFB
Data File : M:\MS2011Q1\LURCH\DATA\FEB11\L021011B\L1100380.D Vial: 1
Acq On : 10 Feb 2011 4:58 pm Operator: tdd/cld
Sample : bfb Inst : Lurch
Misc : Can #1454 "A", lOmL Multiplr: 1.00
MS Integration Params: rteint.p
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
TIC: L1100380. D
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
0
300000
250000
200000
150000
100000
50000
0
18.00 18.20 18.40 18.60 18.80 19.00 19.20 19.40 19.60 19.80 20.00 20.20 20.40 20.60 20.80 21.00 21.20 21.40 21.60
Average of 19.802 to 19.822 min.: L1100380.D (-)
20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210
AutoFind: Scans 1667, 1668, 1669; Background Corrected with Scan 1661
Target
Mass
50
75
95
96
173
174
175
176
177
Rel . to
Mass
95
95
95
95
174
95
174
174
176
Lower
Limit%
8
30
100
5
0.00
50
4
93
5
Upper
Limit%
40
66
100
9
2
120
9
101
9
Rel.
Abn%
15.5
34.7
100.0
6.8
0.0
105.1
7.4
96.5
6.3
Raw
Abn
45731
102693
295829
20002
0
310933
23128
299989
18774
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
EA#" 021T-49' "Page"94 off 06"
L1100380.D L021111A.M
Fri Feb 11 19:06:00 2011
-------
Average of 19.802 to
bfb
Modified:subtracted
19.822 min.: L1100380.D
m/z
29.05
30.95
31.85
32.95
35.95
37.00
38.00
38.95
39.85
42.90
43.90
•age of
abund .
93
10660
401
1773
3100
13900
11678
4273
20
248
1518
19.802 to
m/z
44.95
46.00
46.90
47.95
49.00
50.00
50.95
51.95
54.95
55.95
56.90
19.822 min
abund .
2256
104
1456
1750
10693
45731
14315
581
799
3600
6121
. : L1100380
m/z
57.95
59.90
61.00
61.95
62.95
63.85
66.85
67.90
68.90
69.90
72.00
.D
abund .
423
2424
10055
9629
6835
621
175
22590
20791
1496
1077
m/z
73.00
73.95
74.95
75.95
76.85
77.75
77.90
78.90
79.90
80.90
81.85
abund .
9157
33755
102693
9007
1090
295
480
9056
2245
9432
1737
bfb
Modified:subtracted
m/z
86.85
87.85
88.95
89.25
90.85
91.90
92.90
94.00
95.00
96.00
96.85
Average of
abund .
9731
9295
73
72
1196
7505
12589
33445
295829
20002
496
19.802 to
bfb
m/z
102.80
103.80
104.80
105.85
106.85
110.80
111.85
112.80
114.80
115.85
116.90
19.822 min
abund .
89
1554
632
1463
378
262
190
282
352
1362
2020
m/z
117.80
118.85
122.95
123.80
125.60
126.70
127.85
128.85
129.85
130.80
132.85
abund .
1224
1629
94
163
88
102
1365
529
1338
426
78
m/z
134.85
135.95
136.80
140.00
140.85
141.90
142.85
143.80
144.85
145.80
146.90
abund .
574
72
497
114
2668
283
2398
193
285
551
287
. : L1100380.D
Modified: subtracted
m/z
147.80
148.80
149.75
151.90
152.80
153.75
154.80
156.85
158.80
160.80
170.40
abund .
896
310
289
162
189
162
856
683
286
355
77
m/z
171.20
171.60
171.90
173.80
174.90
175.80
176.80
177.80
206.90
207.90
abund .
72
118
165
310933
23128
299989
18774
429
256
84
m/z
abund .
m/z
abund .
EA# 0211-49 Page 95 of 106
-------
BFB
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021111A\L1100402.D Vial: 1
Acq On : 11 Feb 2011 6:20 pm Operator: tdd/cld
Sample : bfb Inst : Lurch
Misc : Can #1454 "A", lOmL Multiplr: 1.00
MS Integration Params: rteint.p
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
TIC: L1100402. D
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
350000
300000
250000
200000
150000
100000
50000
17.80 18.00 18.20 18.40 18.60 18.80 19.00 19.20 19.40 19.60 19.80 20.00 20.20 20.40 20.60 20.80 21.00 21.20 21.40 21.60
Average of 19.787 to 19.807 min.: L1100402.D (-)
20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210
AutoFind: Scans 1668, 1669, 1670; Background Corrected with Scan 1662
Target
Mass
50
75
95
96
173
174
175
176
177
Rel . to
Mass
95
95
95
95
174
95
174
174
176
Lower
Limit%
8
30
100
5
0.00
50
4
93
5
Upper
Limit%
40
66
100
9
2
120
9
101
9
Rel.
Abn%
16.7
35.6
100.0
6.6
0.0
100.4
7.5
96.7
6.8
Raw
Abn
55899
119432
335189
22186
0
336555
25304
325397
22028
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
EA#" 02"lT-49" "Page"96 off 06"
L1100402.D L021111A.M
Fri Feb 11 19:33:10 2011
-------
Average of 19.787 to
bfb
Modified:subtracted
19.807 min.: L1100402.D
m/z
29.05
30.95
31.95
33.00
35.95
36.95
38.00
38.95
39.90
41.25
42.35
Average of
abund .
114
12914
897
2614
3765
18237
14345
4711
60
67
72
19.787 to
bfb
m/z
42.95
44.00
44.95
46.00
47.00
47.90
49.00
50.00
50.95
51.95
54.90
19.807 min
abund .
244
2250
3143
108
2124
2145
12745
55899
16242
841
828
m/z
55.95
56.90
57.85
59.95
61.00
61.95
62.95
63.85
64.00
66.15
67.00
. : L1100402.D
Modified: subtracted
m/z
79.90
80.90
81.90
83.00
85.90
86.90
87.85
89.55
90.90
91.95
92.95
Average of
abund .
2774
12022
2167
233
307
10280
10469
71
1469
9139
12902
19.787 to
bfb
m/z
94.00
95.00
96.00
96.95
102.90
103.80
104.75
105.00
105.85
106.85
109.95
19.807 min
abund .
38229
335189
22186
505
77
1873
521
189
1606
451
75
m/z
110.90
111.85
112.80
114.90
115.85
116.85
117.85
118.85
122.75
123.90
125.80
. : L1100402.D
Modified: subtracted
m/z
141.85
142.85
143.85
144.75
145.80
146.90
147.80
148.80
149.90
152.80
153.80
abund .
506
2781
103
130
642
402
837
262
338
203
241
m/z
154.80
155.75
156.85
158.80
160.90
171.25
172.10
173.80
174.90
175.90
176.85
abund .
928
200
840
388
221
171
209
336555
25304
325397
22028
m/z
177.85
207.80
abund.
4643
7477
279
2911
12188
11345
8410
242
476
109
1008
abund.
311
140
321
439
1439
2423
1476
1971
70
282
164
abund.
754
97
m/z
67.90
68.90
69.95
72.00
73.00
73.95
74.95
75.95
76.85
77.85
78.90
m/z
126.70
127.80
128.85
129.85
130.80
134.80
135.85
136.85
138.80
139.90
140.85
m/z
abund.
25947
25200
1744
1597
10737
40157
119432
10205
1387
893
11226
abund.
84
1604
474
1571
517
597
202
597
68
325
2739
abund.
EA# 0211-49 Page 97 of 106
-------
BFB
Data File : C:\MS2011Q1\LURCH\DATA\FEB11\L021711A\L1100434.D Vial: 1
Acq On : 17 Feb 2011 2:47 pm Operator: tdd/cld
Sample : bfb Inst : Lurch
Misc : Multiplr: 1.00
MS Integration Params: rteint.p
Method : M:\MS2011\METHODS\LURCH\L021111A.M (RTE Integrator)
Title : TO15 - 72 TCL - Primary "A"
TIC: L1100434. D
2000000
1800000
1600000
1400000
1200000
1000000
800000
600000
400000
200000
0
17^80 1&00 18^20 18.40 1&60 18^80 laOO 19.20 ia40 19.60 ia80 20^00 20^20 20.40 20^60 20^80 21^00 21^20 21^40 21^60
Average of 19.787 to 19.807 min.: L1100434.D (-)
350000]
300000
250000
200000
150000
100000
50000
0
20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270
AutoFind: Scans 1665, 1666, 1667; Background Corrected with Scan 1660
Target
Mass
50
75
95
96
173
174
175
176
177
Rel . to
Mass
95
95
95
95
174
95
174
174
176
Lower
Limit%
8
30
100
5
0.00
50
4
93
5
Upper
Limit%
40
66
100
9
2
120
9
101
9
Rel.
Abn%
16.5
35.7
100.0
7.0
0.0
109.1
7.2
96.0
6.7
Raw
Abn
51523
111720
312811
21914
0
341419
24741
327915
21907
Result
Pass/Fail
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
PASS
EA#" 02"lT-49" "Page"98 off 06"
L1100434.D L021111A.M
Fri Feb 18 13:09:17 2011
-------
Average of 19.787 to
bfb
Modified:subtracted
19.807 min.: L1100434.D
m/z
29.65
30.05
30.95
31.95
33.00
35.90
36.95
38.00
38.95
39.95
40.15
Average of
abund .
89
93
11443
1148
2531
3452
16031
11660
4555
176
76
19.787 to
bfb
m/z
42.85
43.95
44.90
46.20
46.95
47.90
48.95
50.00
50.95
51.95
54.90
19.807 min
abund .
284
2072
2663
109
1832
2075
11925
51523
15119
645
687
m/z
55.95
56.90
57.95
58.70
59.90
60.95
61.90
62.90
63.95
65.35
66.95
. : L1100434.D
Modified: subtracted
m/z
77.95
78.85
79.80
80.80
81.85
82.90
85.85
86.85
87.85
89.05
90.85
Average of
abund .
318
10906
2351
10811
1961
168
271
9183
9345
82
1310
19.787 to
bfb
m/z
91.90
92.90
93.95
94.90
95.95
96.75
96.95
102.85
103.80
104.75
105.80
19.807 min
abund .
9012
13022
36723
312811
21914
188
512
347
1577
637
1713
m/z
106.85
109.75
110.85
111.85
112.05
113.00
114.85
115.80
116.80
117.80
118.80
. : L1100434.D
Modified: subtracted
m/z
131.75
132.85
134.80
135.90
136.75
138.70
139.90
140.85
141.85
142.80
143.75
abund .
69
76
632
164
583
93
300
2690
366
3199
78
m/z
144.85
145.80
146.80
147.75
148.75
149.80
150.80
151.85
152.80
153.85
154.75
abund .
89
645
415
1066
400
254
70
168
173
149
956
m/z
155.75
156.80
157.75
158.75
160.75
169.65
170.70
171.50
171.85
173.80
174.80
abund .
3967
7357
229
74
2481
11145
10206
8183
651
74
793
abund .
332
158
234
167
88
252
388
1404
2171
1419
1813
abund .
71
669
87
465
392
78
171
171
261
341419
24741
m/z
67.90
68.90
69.95
71.10
71.90
72.90
73.90
74.95
75.95
76.90
77.70
m/z
121.75
122.65
123.80
124.65
125.60
125.80
126.70
127.80
128.80
129.85
130.85
m/z
175.80
176.75
177.80
178.65
180.95
190.75
206.75
207.80
208.80
232.00
268.95
abund.
24989
23728
1604
165
1422
10369
37013
111720
9829
1258
383
abund.
90
81
305
155
116
71
106
1598
704
1234
485
abund.
327915
21907
578
87
74
77
227
84
77
75
103
EA# 0211-49 Page 99 of 106
-------
Sequence Name:
Comment:
Operator:
Data Path:
Pre-Seq Cmd:
Post-Seq Cmd:
M:\MS2011Q1\LURCH\SEQUENCE\L021011B.S
tdd/cld
c:\ms2011ql\lurch\data\febll\L021011B\
Method Sections To Run
(X) Full Method
( ) Reprocessing Only
On A Barcode Mismatch
(X) Inject Anyway
( ) Don't Inject
Line Type
Vial DataFile Method Sample Name
1 BFB
BFB
Calibration
1 L1100380
1 L1100301
MM624-L
MM024-L
bfb
4 Calibration
5 Calibration
6 Calibratic
ration
8 Calibration
9 Calibration
10 Calibration
11 Calibration
rZ i^^J _L J^.G
1 L1100382 MM624-L O-^pJliBt-feer-TS^Std A
1 L110038i_MM&a-4-fI oT4ppbv to-15 std A
MM624-L Ippbv to-15 Std A
1 L1100305 MMG21 L 2ppbv to ID atd A
1 L1100386 MM624-L Sppbv tO-15 Std A
1 L1100387 MM624-L lOppbv tO-15 Std A
1 L1100388 MM624-L 20ppbv tO-15 Std A
1 L1100389 MM624-L 30ppbv to-15 std A
-L U J_i -L -^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^pjpjJT^^^IvTi^^iS
13 Spike
14 Blank
15 Blank
16 Blank
17 Blank
18 Cal
Laefe-
10 L1100391 MM624-L Sppbv
1 L1100392 MM624-Lja«fflia"blank
humid blank
13 LlJJ3^3r9^MM624-L AZ gas 038442795
jll00395 MM624-L AZ gas 038442795
1 L1100396 MM624-L 0 . 4ppbv to-15 Std A
1 L1100307 MM621 L — humid blank
20 Calibration
21 Calibration
22 Calibration
23 Blank
1 L1100398 MM624-L 0.4ppbv to!5 Std A
1 L1100399 MM624-L Ippbv to!5 Std A
1 L1100400 MM624-L 2ppbv to!5 Std A
1 BAKEOUT BAKEOUT BAKEOUT
EA# 0211 -49 Page 100 of 106
Last Modified: Tue Feb 15 12:25:33 2011 Page:
-------
Sequence Name: M:\MS2011Q1\LURCH\SEQUENCE\L021111A.S
Comment:
Operator: tdd/cld
Data Path: c:\ms2011ql\lurch\data\febll\L021111A\
Pre-Seq Cmd:
Post-Seq Cmd:
Method Sections To Run
(X) Full Method
( ) Reprocessing Only
On A Barcode Mismatch
(X) Inject Anyway
( ) Don't Inject
Line Type
Vial DataFile Method Sample Name
1 BPB
2 BFB
3 Calibration
4 Spiko
5 Spike in T.I inn inn Mill I T Sppbv ICV/LCS std B
-|_ LllOO'106 MM62\ L. SDDbv ICV/L.CE Qtd E
7 Spike 1 L1100407 MM624-L Sppbv ICV/LCS Std B
—1 L11004U1 MMb^!4-L
1 L1100402 MM624-L bfb
1 L1100403 MM624-L Sppbv tO-15 Std A
10 L1100404 MM024 L
O ±3 J. CU1JI
1 LllOOdOO MMC2(
humid blank
10 Blank
1 BAKEOUT BAKEOUT BAKEOUT
EA# 0211-49 Page 101 of 106
Last Modified: Mon Feb 14 07:15:42 2011 Page: 1
-------
Sequence Name: M:\MS2011Q1\LURCH\SEQUENCE\L021711A.S
Comment:
Operator: tdd/cld
Data Path: c:\ms2011ql\lurch\data\febll\L021711A\
Pre-Seq Cmd:
Post-Seq Cmd:
Method Sections To Run
(X) Full Method
( ) Reprocessing Only
On A Barcode Mismatch
(X) Inject Anyway
( ) Don't Inject
Line Type
Vial DataFile Method Sample Name
1 L11004J
50431
1 L1100432
MM624-L
MM624-L
bfb
bfb
bfb
6 BFB
7 Calibration
8 Spike -
9 Spike
10 Blank
11 Blank
12 Blank
13 Cample
14 Oample
15 Sample
16 Sample
17 Sample
IB Sample
I J-1J.J.UU4J J
1 L1100434
1 L1100435
10 L1100136
MM624-L
MM624-L
DID
bfb
Sppbv to-15 std A
Sppbv LCS ctd A
1 L1100437 MM624-L Sppbv LCS Std A
1 L1100430 MM024-L—humid blank
1 L1100439
1 L1100440
1 L1100111
MM624-L
MM624-L
MM621 L
humid blank
system blank
0211 71 AB 101
1 L1100442 MM024 L - 0211 74 AB 101
1 L1100443 MM624-L 0211-74 AE-101
1 L1100444 MM624-L 0211-74 AE-101 LD
1 L1100445 MM624-L 0211-49 ACB-M0040-FB *5000
19 Sample
20 Sample
21 Sample
22 Blank
23
1 L1100447 MM624-L
1 L1100448 MM624-L
1 L1100449 MM624-L
1 BAKEOUT BAKEOUT
0211-49 1R-ACB-M0040-02 *10,0
0211-49 1R-ACB-M0040-02 *5,00
0211-49 1R-ACB-M0040-02 *10,0
BAKEOUT
EA# 0211 -49 Page 102 of 106
Last Modified: Fri Feb 18 14:23:16 2011 Page:
-------
TOPLEVEL PARAMETERS
Method Information For: M:\MS2008Q3\LURCH\METHODS\MM624-L.M
Method Sections To Run:
( ) Save Copy of Method With Data
(X) Pre-Run Cmd/Macro = MPVGotoVialDDE
(X) Data Acquisition
( ) Data Analysis
(X) Post-Run Cmd/Macro = macro "postmsd.mac"
Method Comments:
This is the acquisition method for Method TO-15 with Bromochloromethane,
1,4-Difluorobenzene & Chlorobenzene-d5 as the internal standards being
used to quantitate analytes.
END OF TOPLEVEL PARAMETERS
INSTRUMENT CONTROL PARAMETERS
Sample Inlet: GC
Injection Source: External Device
Injection Location: Rear
Mass Spectrometer: Enabled
Zone Temperatures: State
Inlet A: On
Inlet B: Off
Detector A: Off
Detector B: On
Auxiliary: Off
HP5890 Temperature Parameters
Setpoint
250 C
50 C
50 C
280 C
50 C
Oven Parameters:
Oven Equib Time:
Oven Max:
Oven State:
Cryo State:
Cryo Blast:
Ambient:
Oven Program:
Initial Temperature:
Initial Time:
Level
1
2 (A)
3(B)
Rate
(C/minute)
5.0
15.0
70.0
Next Run Time:
0.20 minutes
325 C
On
On
On
25 C
30 C
3.00 minutes
Final
Temperature (C)
75
120
240
26.71 minutes
Final
Time (minutes)
0.00
2.00
8.00
HP5890 Inlet Pressure Programs
GC Pressure Units: psi
Inlet A:
Constant Flow:
Constant Flow Pressure:
Constant Flow Temperature
Initial Pressure:
On
8.7 psi
35 C
0.0
Method: MM624-L.M
0211-49 Page 103of 106
Thu Aug 21 13:44:13 2008 Page: 1
-------
1 Testplot 0.053 5.000 0.00 1.00
2 Testplot 0.053 5.000 0.00 1.00
MS ACQUISITION PARAMETERS
General Information
Tune File : bfb.u
Acquistion Mode : Scan
MS Information
Solvent Delay : 3.00 min
EM Absolute : False
EM Offset : 0
Resulting EM Voltage : 1717.6
[Scan Parameters]
Low Mass : 29
High Mass : 300
Threshold : 200
Sample # : 3 A/D Samples 8
END OF MS ACQUISITION PARAMETERS
END OF INSTRUMENT CONTROL PARAMETERS
EA# 0211 -49 Page 104 of 106
Method: MM624-L.M Thu Aug 21 13:44:13 2008 Page: 3
-------
Initial Time:
650.00 minutes
Rate
Level (psi/minute)
1 0.00
2(A) 0.00
3(B) 0.00
Total Program Time:
Column Length:
Column Diameter:
Gas:
Vacuum Compensation:
Final
Pressure (psi)
0.0
0.0
0.0
650.00 minutes
60.00 m
0.320 mm
He
On
Inlet B:
Constant Flow: Off
Constant Flow Pressure: 0.0 psi
Constant Flow Temperature: 50 C
Initial Pressure: 0.0 psi
Initial Time:
Rate
Level (psi/minute)
1 0.00
2(A) 0.00
3(B) 0.00
Total Program Time:
Column Length:
Column Diameter:
Gas:
Vacuum Compensation:
650.00 minutes
Final
Pressure (psi)
0.0
0.0
0.0
650.00 minutes
30.00 m
0.530 mm
He
Off
Final
Time (minutes)
0.00
0.00
0.00
Final
Time (minutes)
0.00
0.00
0.00
HP5890 Packed Column Flow Control
Inlet A not used to control packed column flow.
Inlet B not used to control packed column flow.
HP5890 Purge Valve Settings
Inlet Purge
A
B
Init Value On Time Off Time Splitless Injection
Off 0.00 0.00 Yes
Off 0.00 0.00 Yes
HP5890 Valve and Relay Information
Initial Setpoints:
5890 Valves:
Valve 1: Off
19405 Valves:
Valve 5: Off
19405 Relays:
Relay 1: Off
Valve 2: Off
Valve 6: Off
Relay 2: Off
Valve 3: Off
Valve 7: Off
Relay 3: Off
Valve 4: Off
Valve 8: Off
Relay 4: Off
Detector Type State
A --- Off
B --- Off
HP5890 Detector Information
Not saving signal data.
Signal Source
Method: MM624-L.M
HP5890 Signal Information
Peak Width cP^ft^^f^c
Thu Aug 21 13:44:13 2008
p Data
Page: 2
-------
This Is The Last Page
Of This Report.
EA# 0211 -49 Page 106 of 106
-------
APPENDIX C: BIOLAB RESULTS
C-1
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No,
Technicians Name
Counters Name
1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Lemieux ^
10/26/2010
Burn Hut Wipes
Results
[Date
10/29/2Q1Q|Volume Plated: |lOOuL [Extraction Volume: |20 ml
Tube Dilution
•jgagn^iMsi
^•1
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
C1
C2
W1
W2
W3
W4
Pg
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Q
c)
0
n
0
0
0
C)
D
0
^
r>
C
n
O
Q
Q
Q
Q
0
0
Notes:
'NO A/ - £
. it/yj j Vifa Vf f\ OJc
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchaiian
PI
Test Date
Test No.
Lemieux \J
11/2/2010
Burn Hut Wipes
Date
Sample ID
Results
11/3/2010|Volume Plated: |lQOuL [Extraction Volume:
Tube Dilution
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
C1
C2
W1
W2
W3
W4
FB
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
0
(0
0
n
o
9
/)
0
a
0
0
0
0
Q
Q
0
ft
f)
n
Notes:
Post spor-klenz, incubated at 55° C.
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information '(YC
EPA Project No.
Technicians Name
Counters Name
1-2
Nicole Griffin Gatcnalian
Jason Levine
PI
Test Date
Test No.
Paul Lemieux j* ^
11/17/2010
Bum Hut Dry Run
Date
1
1/18/2010
Volume
Plated:
Results
[100[iL
Extraction
Volume:
varied,
see
comments
Tube Dilution
Sample ID
Gs Spike
Front Rinse
Water Fraction
Spike Oil
Plain Oil
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
A
B
C
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
B
C
B
C
B
C
A
B
C
A
B
C
-VvUL
I
I
O
fi
o
o
n
o
IvUu
I
I
C
C
fi
"~tv"l \~{^
\
Vy
^5
0
0
O
O
-2^
'^o
S3
0
C;
/O
'-"-i^
L ^u|
6l3
g
fi
O
o
^
^
fi>
o
0
0
2
o
f)
o
C
r
^
(j
Notes. Qcff\ (-JLQ, v^rr.wiM; \i0v\Pu (A V\UV\A.U.?JK yv\ L* *
40mli ^wvL^ ^
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information i" ! (
EPA Project No.
Technicians Name
Counters Name
1-2
Nicole Griffin Gatchalian
Jason Levine
PI
Test Date
Test No.
Paul Lemieux ^ v-
11/17/2010
Burn Hut Dry Run
Results
| Date
110O^L | Extraction Volume: |varied, see comments
Tube Dilution
111/18/2010|Volume Plated:
1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
Plate
Sample ID Repl.
Gs Spike
Front Rinse
Water Fraction
Spike Oil
Plain Oil
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
-VvHc
I
I
O
0
O
6
Q
r.
\\\\Ls
I
C
0
C
•\v\\ L
\
6
D
O
O
O
2£3
'2, o
0
O
n
liiifj
140,1^
510,
^
0
O
O
i,
^
G>
0
0
0
C)
0
f)
O
O
r>
0
£
c
fi
O
n
1mL |
100mL collected
extracted from 25m L
5mL
10ml
10mL
Notes: (Jx'
V\UV\AV_W \
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
f Technicians Name
Counters Name
1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
plated 11/22/2010
Burn Hut Dry Run 11/17 samples
| Date
r\6SUitS
Volume Plated: hOOnL I Extraction Volume: (varied, see comments
11/23/2Q1Q
Tube Dilution
Sample ID
Gs Spjke
Bones
Spike Ash
Plain Ash
Negative plates
Notes: <^0\\£y
4-W V
Plate
Repl.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
j IYIL^ v^c
1.E+00
0
a
o
'fWfk
32
£>
O
l>
\
\
c>
+V\L (•&
nn r^s\\
1.E-04
QDl^LC
am uifi-*
Comments
ImL
1.54g of bones
inlOmLPBST
0.67g of ash
in lOmL PBST
0.82g of ash
in 10mLPBST
QC media check
at55C
ndf\6 A -V-n
^c\^ Y\rAtr\ fti^-
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
( Technicians Name
} Counters Name
1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
plated 11/22/2010
Burn Hut Dry Run 11/17 samples
Results
Date
11/23/2010] Volume Plated: |100^L | Extraction Volume:
varied, see comments
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1 .E-04 comments
Gs Spike
Bones
Spike Ash
Plain Ash
Negative plates
A
B
3
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
p
£>
114 ^U
•ftJfL
•rW'TL
0
0
ft
6
O
O
•fr-lTC-
ftsrTC
T»4*To
fi
0
n
[A*k
10
6
0
o
•"irO-fC'
TKlt^
"fivlTdx'
n
fi
fi
•q
t^
t
0
o
C
\i^3
Ll*'O_
•3,1^
I
\
6
1mL
1.54g of bones
in 10mLPBST
0.67g of ash
inlQmLPBST
0.82g of ash
in 10mL PBST
QC media check
at55C
Notes: <^p\\£/ $Sln SCUMiOU, ^&M lfY">jiUL flC 4V\£. fir4^ f/sr\VCLm Ul<5rK"£v^ Wi-VdrV -PtV^
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
plated 11/22/2010
Burn Hut Dry Run 11/17 samples
Results
I Date
111/23/20101 Volume Plated:
100nL [Extraction Volume: [varied, see comments j
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-04 comments
Gs Spike
Bones
Spike Ash
Plain Ash
Negative plates
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
0
TVVTG
TVJTG
TViTG
o
6
O
0
0
a
'TKiTG
"YVKG-
tVtfG
D
0
0
[ ~
^0
(£,0,
o
e>
ft
O
M-^
aa
?>u
i
\
o
1mL
1.54q of bones
in 10mL PBST
0.67g of ash
in 10m L PBST
0.82gof ash
in 10mLPBST
C media check
at55C
Notes: MO ftaf\^M\f\\\f\d^f\ar\ $,?fy\
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gaichalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
1/25/2011
1/20/2011 AC B Test
Results
Date
1/26/2011 1 Volume Plated: |100 uL (Extraction Volume:
vanes
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
01201 1-WIPE-W1
012011-WIPE-W2
012011-FL-ASH1
012011-FL-ASH2
012111-ACB-ASH
012111-ACB-CC
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
n
Q
o
~
0
^
\ ^
\ •£
L>
q
o>
Q
ft
O
O
o
I
&
o
o
n
S)
£>
-z,
3*
A-
\
Q
cctfvv *•
o
F\
O
1
\
I
D
o
n
O
^
•D
Q
G
a
o
•
n
o
fr
O
d
f^
<-}
^
(^
f\
,,.
f^
Q
0
^
^
^
Q
n
Q>
Q
,5
£)
o
&
ft
^
C)
a
f>
o
e>
~&
o
a
&
o
6
&
used 20mL PBST
for extraction
used 20mL PBST
for extraction
used 40ml_ PBST
for extraction
used 40mL PBST
for extraction
A.lCuuun c$ Jfamos
!.02g of ash in
20mL PBST
for extraction
2.55g of bones
in 20mL PBST
for extraction
\\.°(
Notes: Incubated at 55°C for Geobacillus stearothermophilus
< ri
rif 1 e>i/AO
« j
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
\ EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchatian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
2/17/2011
2/16/2011 AC B Test
Results
Date plated
2/1 8/2011 1 Volume Plated: |100 nL (Extraction Volume: (varies |
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
021711-WIPE-W1
021711-WIPE-W3
021711-FL-ASH1
021711-FL-ASH2
021711-ACB-ASH
02171 1-ACB-CC
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
A
2
a
D t$M
0
E
Q
0
ft COM
0 COCA-
f) (CV\k
D
f)
n
0
D
2
A
ft
A
&
t)
A
D
A
A
Q.
/S
O
(5
n
D
r>
o
6
D
n
6
n
Q
6
Q
0
ft
£>
fr
b
ft
5
n
o
fi
ft
20mL PBST
for extraction
20mL PBST
for extraction
40mL PBST
for extraction
1.61g of ash
40mL PBST
for extraction
1.01g of ash
>OmL PBST
for extraction
1 .44g of ash
20mL PBST
for extraction
1.57g of bones
Notes: Incubated at 55°C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
2/24/2011
2/23/201 1 ACB Test
Results
[Date plated
I 2/24/20111 Volume Plated: |100 ML [Extraction Volume: [varies |
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
02241 1-WIPE-W1
02241 1-WIPE-W3
02241 1-WipeBlank
02241 1 -RINSE
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
o
f}
f)
ft
A
o
f)
f)
/)
n
f>
0
n
d
d
n
fj
0
n
6
0
/}
0
o
0
f)
n
20mL PBST
20mL PBST
20m L PBST
1 0OmL glassware rinse
with, sterile Dl water
Motes: Incubated at 55°C for Geobacillus steamthermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchaiian
PI
Test Date
Test No.
Paul Lemieux
2/22/2011
2/22/2011 sterility check
Results
Date plated
12/22/201 llVolume Plated: |1QO iiL |Extraction Volume: [varies
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
02221 1-WIPE-W1
022211 -WIPE-W1
02221 1-WIPE-W1
02221 1-WIPE-W2
02221 1-WIPE-W2
02221 1-WIPE-W2
02221 1-WIPE-W3
02221 1-WIPE-W3
02221 1-WIPE-W3
02221 1-VialTrap
Neg Control
A
B
,-
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
0
0
G
O
O
O
ft
O
n
f)
O
0
r>
u
n
O
A
fiilitKm piflLti
1 ml. filter plate
10mL filter plate
rWYUHnn p\f\ TJL
1mL filter plate
10mL filter plate
(AtYUttlAA OVflLJ-L
^^
1 mL filter plate
10mL filter plate
Notes: Incubated at 55°C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
2/24/201 1
02241 1 AC8 Test
Results
plOO iiL [Extraction Volume: ; varies
Tube Dilution
[Date plated Ufefel -g/g4/se44|Volume Plated:
Plate
pH Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
\a.yi
I3.<4o
U-W
U.04
Ui.^U
l.tt
WcclVajp
>
r>
£j
(5
O
G
O
(^
O
Q
e>
s
o
0
o
Q
0
Q
0
n
0
o
r)
O
£>
d
G
O
Q
G
O
C)
6
O
o
{-•)
o
o
f)
f)
f)
ft
t>
Q
o
G
D
O
f>
*£)
O
D
o
&
o
Q
O
^
D
I^^YIL- op ni\
\MfVf Vt(fl\ltVtd
no_ji>V-
\<\ UOCML PfiST
fto ijOh
lA LVooiU PftU P6fe,T
\OOmuoP t>t>Vv)
Vf\a<^a WArb\^>A
i»0.'5x'5xMesor<,
^
Notes: Incubated at 55°C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1
of 1
Test Information
^&
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux " w
<3Ll&?>l\V
£>- &C&-BS-63-
Results
Date plated
^\\\\\ | Volume Plated:
ICHuLt- | Extraction Volume:
\/ft/»>A
COUAHd 3 1 7} \ \ Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
a-pcg-'&S'oa
Oi\
£- ftc&-BS-oa
t^CnS'C
t4ft
o
r>
&
O
o
0
o
o
fi>
o
€>
e>
&
G>
ft
r~}
liSmt rem/ev^d
\oomu DDVAJ
ViA'st CoUtC-Vtcl
Notes: Incubated at 55"C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Page 1 of 1 U \(//Y\
— 1 N
\j \^j
Paul Lemieux
3/1/201 1
030111 ACBTest
Results
[Date plated
In ]Volume Plated: llOO^iL [Extraction Volume: [ varies '
Tube Dilution
Sample ID
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
030111 ACB ASH
030111 ACBCC
030111 FLYASH 1
030111 FLYASH 2
030111 WIPE W1
030111 WIPE W3
030111 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
ft
0
0
6
f> MM
fl fflft-V
0 rwyil
5> tc>ot
*r* rftnV-
2. trf\\
0
fi
D
ft
h
0
(^
Q
0
0
0
0
0
0
0
0
0 (£V\t-
fl fflttV-
0 tOAf
o
0 utf\\
Q
Q
O
n
r^
0
n
0
D
s
n
0
0
n
Q
6
O
o
ft
n
n
o
O
0
1 • OOo ftSVi » t^
3fWL PftST
X.l3*a InriO^ i^\
^Orv/L Pftf?tT
l-6Ma OCiVi oo |
W£'PAJ irt UOmu
pes-r
I • ^T-A &£V\ w>
WfePDL^fn »4ovviu
Pfe^T
Stomv P(2£T
^LfVrtL. P^ST
3^m^ PeST
Notes: Incubated at 55°C for Geobacillus stearothennophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
SSS Gln'tfyn fcTLtUAAJLiYtL,
PI
Test Date
Test No.
Paul Lemieux
3JI
It
&<;-
&
ft
o
o
o
(
o
\amL vKotfxeft
\oorflu T^^*vo
V"\Vid f (~f) \ \p c.\ff\
\TM '^(b
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Oriffin Qdtdidlidn Cc,
Nicole Griffin Gatchalian
PI
Test Date
Test No.
PJU! Lemieux
3/2/201 1
030211
Results
[Date plated
I%\%\\V |Volume Plated: [100 iiL Extraction Volume: .varies
Tube Dilution
2O m U
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 c««mnts
030211 WIPEW2
03021 1 WIPE W4
030211 WIPE BLANK
A
B
C
A
6
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
0
A
A
n
fl
o
0
0
0
0
0
ft
0
0
5
o
0
0
Notes: Incubated at 55°C for Geobacillus stearothermophilus
V->LA
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No,
Technicians Name
Counters Name
WA1-2 rte^.p
Nicole Griffin Oatchalian cs
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/3/2011
030311 ACB Test
030311 ACB ASH
03031 1 ACB CC
030311 FLYASH1
030311 FLY ASH 2
030311 WIPEW1
03031 1 WIPE W3
030311 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
b
n
r>
0
A
IL fon4-
A\ rpn V
\\ fflA*
n
fl r-rViv-
o
0
d
r>
0
n
O
n
Q
D
ft
n
A
6
0
Q
Q
0
a
n
5
0
f^
o
O
n
Q
n
n
Q
A
fi
n
A
o
n
r>
Q
n
n
i^
5
o
o
Q
Q
^.O^U op a^lo
iA 8QDm L PfiST
M-.llci eK- hortf?
ir» Somt P\^ST
uHZ
uC| WEPlR iV
Uomt P6ST
1 .^I4£K
uJl UfePV^ iVx
^•Ornu PBST
:Smu Pft&T
ao \mu PV^ST
"^NO^pnv Pft^T
Notes: Incubated at 55°C for Geobacillus stearothermophifus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/4/2011
030411
Results
JDate plated 2>|
\4 \l (Volume Plated:
Plate
Sample ID Repl. 1.E+00
1.E-01
(100
1
(Extraction Volume:
Tube Dilution
.E-02 1.E-03
1.E-04
• voncs
Comments
030411 WIPEW2
03041 1 WIPE W4
030411 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Notes: Incubated at 55°C for Geo
fj
0
n
a
0
6
n
n
0
O
O
p
0
n
n
bacillus stearothermop
n
n
P
o
0
7)
Was
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
NX • t±3Y\ ^CiVN £~r£XjA~I-V"\.C«JL • OM-\
PI
Test Date
Test No.
Paul Lemieux
m
u
5-BG&-&S 'OM
Results
Date
"i>\\o\\\ |Volume Plated: jtOOpiL | Extraction Volume:
VdAd S
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
O\\
a — \\f O-— Q^.—^\i \
r\*~ <^ WO L^^"l
«,«,
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
O
G>
O
3
o
£,
a
o
a
ft
O
o
G
&
O
O
o
O
o
Ct
o
IL^SwlY^ fy\
^Q(r\iOveA
\OO (V\\- bOVsl
V»VA*^<
totA CS>
UCcCiVcup
^tvS\;-«-v cvca<-
Notes: Incubated at 55°C for Geobac/V/us steamthermophitus
\)\O
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
WA1-2
PI
Pajl Lemieux
Technicians Name
Test Date
Counters Name
Test No.
Results
| Date plated
?>'^ 1 1\ |Volume Plated: |100nL |Extraction Volume:
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04
o\\ A£Q>
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
£>
o
.varies.
VtcCHrfijp oil
Comments
\faxTXVjlj0
<4txVViH*
CV\t£JU^
Notes: Incubated at 55°C for Geobacillus stearothermophitus
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
WA1-2
Christina Sione
Nicole Griffin Gatchalian
PI
Test Date
Test No,
Paul Lemieux
3/9/2011
03091 1 ACB Test
Results
| Date plated
1> \t>\\\ |Volume Plated: |100nL | Extraction Volume: varies ]
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
030911 ACB ASH
030911 ACBCC
030911 FLYASH1
030911 FLYASH2
03091 1 WIPE W1
030911 WIPE W3
030911 WIPE BLANK
A
B
C
A
B
C
A
B
C
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
n
0
0
0
£
o
a
i
f>
I
0
&
o
n
z
n
n
/•>
Q
&
O
n
O
ft
n
G
n
n
0
n
n
0
h
n
n
7}
o
n
ry
r>
r>
K
\ -0*Sia
in 9omu Pfi&T
A.o5« tJ«*V\V3ov^
m aomi- PP>S~r
I . ^O« aSh a.r\d
vACPft 4oC(c \v^
*^o m ^ P9>ST
^•^a Q.'iV-k mu PSST
Notes: Incubated at 55°C for Geobacillus steamthermophilus
-------
Serial
Dilution/Plating Results Sheet
EPA Project No.
Technicians Name
Counters Name
Test Information
WA1-2
Niicd-c
KiicdW
(•y Grtit V">
Gr GfTVrOt
\o\ \
fir
Date plated
Sample ID
Results
[Volume Plated: [100 \iL [Extraction Volume: -vaffes ]
Tube Dilution
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
Ao ^ U P&ST
<*«>,. ^,Pe ux»
o^iou uo\pe U3U
6>\OUAVL
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
n
0
o
n
ft
A
n
£)
O
n
O
O
n
£,
O
o
C)
O
n
O
n
Notes: Incubated at 55°C for Geoibacf 'us stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information U ( 1
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Galchalian
Nttol6£?VV£fYV» fo&Jt>i£Uiiu*v
PI
Test Date
Test No.
Paul Lemieux V
Sllolt 1
2\~ ftcii- SS'O?>
Results
Date | ?>lvo\ \\|Volume Plated: |lOQnL |Extraction Volume: |
Vcoa e_s 1
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
&c&-es-c£
OiA
A-6eer$&-os
\2mst
oi\ ne^vt
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
o
r>
f}
&
£
O
r>
o
Q
rt
o
n
r>
o
ft
£>
r>
n
D
•D
\&.
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Christina Slone
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux ^ ^
3/11/2011
031111 ACBTest
Results
Date plated | a.!'. !,[•' [Volume Plated: |100 ill [Extraction Volume: | varies ]
P\-\ Sample ID
Tube Dilution
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
031111 ACB ASH
031111 ACBCC
031111 FLYASH1
031111 FLYASH2
031111 WIPEW1
031111 WIPEW3
031111 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Q
0
0
n
n
0
A
g
A
ft
(S
h
0
fi
Q
Q
Q
n
Q
o
A
A
|
ft
A
B
Q
D
ss
0
L1
Q
ft
0
ft
P
n
Q
5
Q
n
Q
0
0
6
Q
fl
0
C
jj
A
0
6
n
n
5
A
1,0^ Ofi^ i,A
fiomu, pfas-r
^.<&>C>a \o0r\t-
Jn 'ZOM U- ffaST
l-\l*>*5 OSV» uJ|
U6Pft «50/LU- m
Lto^ L pftsrr
um c\ a.-4v\ wi j
"rl'£PfrJ«^C\^ iVj
MOiMb Pfe^T
7J5 mt Pft^T
a^mu Pfi&T
aomu ^fts~T
\\.\l
\\-oo
Notes: Incubated at 55'C for Geobacillus stearothermophitus
-------
Serial Dilution/Plating Results Sheet
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
-Mtcolo Griffin Gatchalian ££
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Pagel of 1 MF
f
Paul Lemieux
3/14/2011
2-A.CB-BS-06
0
Results
[Date plated
\\ |Volume Plated: |100nL [Extraction Volume: | varies
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
2-ACB-BS-06 Oil
2-ACB-BS-06 Rinse
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
r>
o
0
n
O
/s
r>
n
0
r>
n
n
£
n
C)
ft
n
n
'
M&.'Smu oil
V£LCv£r/ 6
lCK*>*V|L D^AA)
VjfKt^
Notes: Incubated at 55°C for Geobacillus steamthermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
GVWsttYUL c=te>v\<.
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Psul Lemieux
3/14/2011
031411
Results
I Date plated
vx [Volume Plated: 1100 ^L |Extraction Volume:
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04
031411 WIPEW2
031411 WIPEW4
031411 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
o
C
O
I
5
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
P,VWlXt\VUL ?>\OlAC
Nicole Griffin Gatchalian
PI
Test Date
Test No,
Paul Lemieux
3/15/2011
031511 ACBTest
[Date plated
_ Results _
[Volume Plated: |lOOnL ] Extraction Volume:
varies
Tube Dilution
Plate
D\\ Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04
fca,i44
u.n
\a.^a
031511 ACBASH
031511 ACB CC
031511 FLYASH1
031511 FLYASH2
031511 WIPE W1
031511 WIPEW3
031511 WIPE BLANK
A
B
•~-
A
B
F
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
0
f)
0
5
/) rpfU
0 (xw
n rmr\+
n fern
0 w\\\
0 con*
l cbnt
A60A^r
o
0
D
Q
0
0
o
o
n
0
0
0
^
D
fl
Q
0
0
A
A
Q
n
r)
0
n
A
0
Q
0
0
2
A
0
5
A
n
/>
2
0
Comments
O-^tfA AsK
irt TowiLPSST
4 • I 0 ft lOOAt.
io lnvv»UP0^r
\ i o'o^i iv^
Ho w\\^ P66t
(j»| ^ocV-1])
{^t&0^ »IIA
i^OmV PfeST
Cv^l sodc^
^Ofvit pe&T
10 OIL P6ST
tOrwt P«ST
Notes: Incubated at 55°C for Geobacillus stearothermophilus
=¥-
CMorues
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
NUCOlt GinfF-Yi GrOiLWMicLn
Nicole Griffin GatchaHan
PI
Test Date
Test No.
Pajl Lemieux
3/16/2011
031611
Results
| Date plated
U I Volume Plated:
|100
| Extraction Volume: | 20mLPBST |
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
031611 WIPE W2
031611 WIPEW4
031611 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Q
fi
0
0
0
0
Q
£>
/*>
Q
e>
0
0
o
o
d
r>
o
o
fi
£3
Notes: Incubated at 55°C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/16/2011
2-ACB-BS-07
Results
Date plated
3/22/2011 1 Volume Plated: |100 nL | Extraction Volume: varies
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
2-ACB-BS-07 Oil
2-ACB-BS-07 Rinse
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
O
O
O
r>
&
r>
o
o
F>
O
6
Q
o
r>
O
&
r>
a
M3>.6*viu
vtmtfyitd
\Ol> mU IN^fcO
vi/Y^
Notes: Incubated at 55°C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
MitoLf ttnPf-n GfoiiiAfritcuv,
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/17/2011
031711 ACBTest
Results
[Date plated | gJaaJn |Volume Plated: |100 ^L [Extraction Volume7
varies
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
031711 ACBASH
031711 ACBCC
031711 FLYASH1
031711 FLYASH2
031711 WIPE W1
031711 WIPE W3
031711 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
€>
O
-V^.13
'•?>
n
ft
0
G
5
0
n
p
0
O
0
O
33
i jx
Q
0
0
1
0
£
fi
0
D
f>
A
£)
Q
O
6
0
^
0
o
o
o
<0
o
o
Q
0
0
A
A
0
O.ftfta o&^>
»V 2onaL PBf^T
^- HSa tawv.
i rt XC5 rv*u P6ST
\,-q.Lt_(a et&Vi \V
V-fOvTrtU PB>S"r
Cy^[ H£P ft SocAt.^
\ i C^A a&v% »v»
4 O vrvC* PfeST
( ui 1 »46PPr So^"3
jj^O ^r>V (^SST
3LO(T¥VL, Pl3ST
SDOnU P6^T
Notes: Incubated at 55°C for Geobacillus stearothermophilus
\T)1A
i^CL,
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
t4\ftflf 0*1 t£»v-» &a±tirv£JUew>-
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/18/2011
031811
Results
(Date plated
u
"£z4\ v I Volume Plated:
MOOuL
[Extraction Volume: |
Tube Dilution
20mL PBST j
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
031811 WIPEW2
031811 WIPEW4
031811 WIPE BLANK
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
ft
£
7)
Q
f>
D
O.
0
o
ft
f)
A
•f>
/.J
o
Notes: Incubated at 55°C for Geobadflus stearothermop<
n
o
n
r>
hilus
-------
Serial Dilution/Plating Results Sheet
Page 1
of 1
Test Information
.4Y&
EPA Project No.
Technicians Name
Counters Name
WA1-2
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/21/2011
2-ACB-BS-08
Results
iDate plated | 3/22/2011 [Volume Plated: |100 uL [Extraction Volume: | varies [
Tube Dilution
Plate
Sample GD Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
2-ACB-BS-08 Oil
2-ACB-BS-08 Rinse
A
B
mf
A
B
C
A
B
">
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
0
O
o
&
D
ft
O
D
0
O
o
7J
£>
<3
0
6
r>
AJtSu^mv
H££V^y-£/l
lOO tfYH^ DDVs/
fCv^Sc-
Notes: Incubated at 55"C for Geobacillus stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name C3> J>^
Counters Name
WA1-2
fVyMiCQie-Griffin Gatchalian
^TJicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux v
3/22/2011
032211 ACBTest
Results
Date plated
Volume Plated:
100 nL
Extraction Volume:
varies
Sample ID
Tube Dilution
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
OA\
03221 1 ACB ASH
03221 1 ACB CC
032211 FLYASH 1
03221 1 FLYASH 2
032211 WIPEW1
032211 WIPE W3
03221 1 WIPE BLANK
A
B
^
A
B
Ml
A
B
z
A
B
2
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
ft
0
6
O
0
n
o
t>
f^
r>
D
O
0
o
£j
G
0
f)
^
n
0
O
6
r>
O
0
0
r>
ft
f)
0
o
0
0
o
o.
r>
O
0
o
o
0
n
0
a
6
o
o
0
o
\v^ ICwnL, ?B^T
2-j^Sov VOD»"\
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information U (
EPA Project No.
Technicians Name
Counters Name
WA1-2
^Jf\i fef irtfiL Stom 6
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/23/201 1
032311
Results
i i rvi mo
[Date plated | 2>|'M [n [Volume Plated: |100JiL~ I Extraction Volume: | 20mL PBST
* T*<.i-.«-5 r~\iiii4imi
Tube Dilution
Sample ID
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
032311 WIPEW2
032311 WIPE W4
03231 1 WIPE BLANK
A
B
c
A
B
2
A
B
3
A
B
f^
M
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
6
A
0
1
0 CXMt-
1
fl
A
o
ft
fl
0
A
O
0
6
0
0
O
ft
D
ZnrvM, PftST
JJ.} tfwu t^oST
Of) irt/^ \f Plp»& i
Notes: Incubated at 55"C for Geobacillus stearothermophilus
-tfusat* cchfrtu <&br\ rm scurvLrtW, o^a^u \j3itx- vc»v4-
^1 '
-------
Serial Dilution/Plating Results Sheet
Page
1 of ft
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Jason Levine
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/29/11 filter plated
wipe filter plates
Results
1
Jppfl
^
Date plated 1 3/29/2011 (Volume Plated: [varies I Extraction Volume: | 20 ml |
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
flVmi
Mpt-Wl
OQL54U -dSViM >
Vwrpt-w3
03o2-i^ -
O^o?- it-
03&3U-
01>^- wj
03oMli-
03o*4M-
vvKpt-M
owU*-*&
oSton-
°'1^ - vW
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
ft
Own*.
0 t rtf\\
bc(A\-
0 fflrtV'
A /«y)^
B
n
0 uvu
o TMV
0 rmv
0 C*^V^
0
0 dv\^
0 tjv^-lr
0 (nf\\
0 CfJV\Y
0 ftj^"f
)) f<*TkV
"Z-L^
Tkl'T'O
Imb
\?. m u
I ¥Y\U
\p,VYlb
I rtlL
l\ wVl/1
1 t>kV/
J^>rv^L'
I WMf
l^rt^L
1 W)V
|-^^\/
1 W\|_,
\JLvVlU
ImL
ImL
nay*
\tv\u
l^mU
\tviu
l^-Siv^^
Notes: Incubated at 55°C for Geobacillus stearothermophiius
-------
Serial Dilution/Plating Results Sheet
Page
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Jason Levine
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/29/1 1 filter plated
wipe filter plates
Results
Date plated
3/29/2011 1 Volume Plated:
varies
Extraction Volume:
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
(VMUl'INvpt'Wl
OW-Wv0t'Wb
WWtt-
O^WW •"
0&\$ll -
yOCpt ' W l
O^l^U *
Wfp6''W3
0?>\lf\l ~"
03lU»U-
\$\Qis~ WH
0^\^-;\-
V^fpC' V^
03 W~
omn-
°w^'wtv
A
B
D
A
B
C
A
B
3
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
5
5
0 ft/It.
0 (MU
\4
1\
l/fo
Trrfr,
0 CoA^
Iprtjf^-
0 (ftp.*
0 fW\*
6 fitfW
o rwAV
o ^^^
0 OMf
0 TttAV
ft C£VH
/! f^AAV
n rfwv
IWrtL-
IfirrtL
irwb
\ ry\U
lOfvlW
IfirvL/
^ mL-
1 wiU
3rt\L
\ VWV>
\OrnU
^tv^t
C^.C^.fVlx-'
YVYIL
^.^.rnu
IWIL
^i4VY>l-
V^VIL
\ fWb
\0*Yllx
IVYIlx
A-^>rr\i>
Notes Incubated at 55"C for Geobaciltus steamtheimophilus
fl^Wtt AVUA; WiMtv fAlOflfCS-
-Vo
-------
Serial Dilution/Plating Results Sheet
Page
Test Information
EPA Project No.
Technicians Name
Counters Name
WA1-2
Jason Levine
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
3/29/2011 filter plated
wipe filter plates
Results
Date plated
3/29/2011 1 Volume Plated:
varies | Extraction Volume: 20 mL |
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
032£ii-
Oja>\\-
°^ipc-^
°^Vpe-WM
A
B
-\
A
B
3
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
6 f^tn\f
& (JW/*
6 (LW
0 tvrtf
Q f£/\t
A tt)\^
ft trv\±
n ttv\A-
Ivwu
IvWL
l^^WL-
\KVlU
°\. JWU
V. VWV^
\\ VV^LX
Notes' Incubated at 55°C for Geobacilius stearothermophilus
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
2-14
Nicole Griffin Gatchalian
Christina Slone
PI
Test Date
Test No.
Paul Lemieux
10/20/2011
SKC viatrap oil spike
Results
Date Plated
Date Counted
10/20/2011
10/21/2011
Organism
Volume Plated:
Gs
100 til
Temperature
Extraction Volume:
55 °C
15 ml
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
SKC-E4
VT-E4
PBST-E4
E6 inoculum
sterile VT
sterile EtOH
sterile PBST
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
Ibft
\M
25
IHI*
m
\qu
Mtf
M&
oLW
0
fi
o
()
0
c>
Ifc
L>
li.
£
fl
to
$£
2&
m
iKfr
|
1
"i^itL
5
wo
fc&fi
MP>
1 5mL of viatrap oil
in SKC biosampjer
spiked at E4
15mL of viatrap oil
in a conical tube
spiked at E4
15mL of PBST
in a conical tube
spiked at E4
6mLprepof29%
athanpj E6 prep
viatrap oil stock
sterility check pjated
sterile ethanol stock
sterility check plated
15mL PBST
sterility check filtered
Notes: The ViaTrap Oil was difficult to plate and the viscosity caused some minor clumping on the plates;
however, the colonies were still discernable.
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
Technicians Name
Counters Name
2-14
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
11/19/2011
Inoculum 1 and 2
Date Plated:
Date Counted:
11/19/2011
11/21/2011
Organism:
Volume Plated:
Gs
100nl
Temperature:
Extraction Volume:
55°C
20 mL
Sample ID
Tube Dilution
Plate
Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
Inoculum #1
Inoculum #2
Negative ViaTrap
Notes:
A
B
z
A
B
"•
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
f}
fi
n
|
J
"TK1T6
k
Mi
Sj£
25
(£>^
L\
(0
Q
15
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
2-14
PI
Paul Lemieux
Technicians Name
Nicole Griffin Gatchalian
rest Date
11/28/2011
Counters Name
Nicole Griffin Gatchalian
Test No.
Inoculum 3
Date Plated:
Date Counted:
11/28/2011
11/29/2011
Organism:
Volume Plated:
Gs
100 ill
Temperature:
Extraction Volume:
55°C
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
Inoculum #3
A
B
j»
A
B
uf
A
B
r>
A
B
r*-
Ui
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
tvrc
23
ii i
£H
Notes:
-------
Serial Dilution/Plating Results Sheet
Page 1
c, 1
Test Information
EPA Project No.
Technicians Name
Counters Name
2-14
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No,
Paul Lemieux
11/19/2011
Inoculum 1 and 2
Date Plated:
Date Counted:
11/19/2011
11/21/2011
Organism:
Volume Plated:
Gs
100(11
Temperature:
Extraction Volume:
55°C
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
Inoculum #1
Inoculum #2
Negative ViaTrap
A
B
z
A
B
•»
A
B
^f
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
3
&
f\
^\tfL
\
t
TN\T6
•^
S
Mi
L-3_
Z.^
3£
23
a
ia
$£
ID
S
i£|
Notes:
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratoiy P.O. #
USEPA
RTP,NC
Burnhut Exhaust Duct
RN990272.0014
BioLab
Sample ID
ipl
Sample Matrix
Analysis Requested
VC-1
BS-1
Wipel-112111
Wipe2-112111
WipeS-112111
Wipe4-112111
BlankWipe-112111
Via Cell
3io Sampler
spores
spores
spores
spores
spores
spores
spores
Notes/Comments:
zzr
Relinquished By:
Relinquished By:
Date/Time:
"/*'/'/
Date/Time:
Received By:
Received By:
Date/Time:
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919)544-4535
Fax Number: (919)544-5690
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
2-14
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
11/21/2011
Testl
i 1
Date Plated:
Date Counted:
11/21/2011
11/22/2011
Organism:
Volume Plated:
Gs
lOOjd
Temperature:
Extraction Volume:
55°C
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1 .E-04 comments
VC-1
BS-1
WiDel-112111
Wipe2-112111
WJpe3-112111
Wipe4-112111
Blank Wipe-
112111
Rinsate- 1
ViaTrap Negative
Notes:
A
B
„/
A
B
-*
^
A
B
n/
A
B
L>
A
B
r1*
LJ
A JftftA
B ^
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
^
0
\
'£,
~3
§
5
Q
D
fl
fi
Q
C
6
0
-£ i
6
0
ET
ft
D
A
0
6
A
0
Q
Q
Q
0
n
tf
/>
Q
0
A
A
0
0
|
UDmU Pft<;T
IDOpi^
vi^br^
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
USEPA
RTP,NC
Burnhut Kxhaust Duct
RN990272.00I4
BioLab
Sample ID
VC-2
BS-2
Wipel-1 12211
Wipe2-112211
Wipe3-l 12211
Wipe4-l 12211
BlankWipe-112211
^att-Q-
Sample Matrix
Via Cell
Bio Sampler
JTvivr* ^\(XS<;v\javc
Notes/Comments: [
Analysis Requested
spores
spores
spores
spores
spores
spores
spores
LkXY\b pgK4. C^f^Lu^gy^ *VL nfe^/n
Relinquished By:
Date/Time:
Received By:
Date/Time:
/ZTfrfafa ^^(it&L-*
(/ ///3-z-///
(l^l.rJ in^o^j^ J
T ///^^ UlU 53OO^,
Relinquished By:
Date/Time:
Received By:
Date/Time:
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919)544-4535
Fax Number: (919)544-5690
-------
Serial Dilution/Plating Results Sheet
Page 1 of
Test Information
iPA Project No.
2-14
PI
Paul Lemieux
Technicians Name
Nicole Griffin Gatchalian
Test Date
11/22/201'
Counters Name
Nicole Griffin Gatchalian
Test No.
Test 2
Date Plated:
Date Counted:
11/22/2011
11/23/2011
Organism:
Volume Plated:
Gs
100|J
Temperature:
Extraction Volume:
55°C
20 ml
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E -04 comments
VC-2
BS-2
Wipel-112211
Wipe2-1 12211
Wipe3-112211
Wipe4-1 12211
Blank Wipe-
112211
V
B
j
A
B
'
\
B
•»
A
B
-
A
B
Q
A
B
/•*
\^
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
5
3
3
1
2
2
o
o
\
z
h
6
0
G
C)
n
t
fl
A
8
Q
D
6
0
fi
0
0
fl
t
B
0
fi
A
Q
fl
C
o
S
rj
l(.0)(UAx
uvvVw/i^p
-------
Serial Dilution/Plating Results Sheet
Page 1 of 1
Test Information
EPA Project No.
2-14
PI
Paul Lemieux
Technicians Name
Nicole Griffin Gatchalian
Test Date
11/28/201'
Counters Name
Nicole Griffin Gatchalian
Test No.
Inoculum 3
Date Plated:
Date Counted:
11/28/2011
11/29/2011
Organism:
Volume Plated:
Gs
100nl
Temperature:
Extraction Volume:
55°C
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
Notes:
V
B
Q
(
B
A
B
'
A
B
^
A
B
*
A
B
C
A
B
C
A
B
C
A
3
C
A
B
C
A
B
C
A
B
C
-tvrc
I
A
33
ii \
55
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
USEPA
RTF, NC
Burnhut Exhaust Duct
RN990272.00I4
BioLab
Sample ID
vc/5
Sample Matrix
Via Cell
Bio Sampler
Notes/Comments: |
Analysis Requested
spores
spores
Via-Cell has extra parts to it this time. All portions of the sample collection train are in the same bag.
jf
Relinquished By:
Date/Time:
Received Bv:
Date/Time:
/y^j/tt *?l**^-A^
\J ///2.4/y/
^^A£;
-------
CHAIN OF CUSTODY RECORD
Project Name
Project Location
Sampling Location
Project Number
Laboratory
Laboratory P.O. #
USEPA
RTP, NC
Burnhut Exhaust Duct
RN990272.0014
BioLab
Sample ID
Wipel-1 12911
Wipe2-1 12911
Wipe3-l 12911
Wipe4-112911
BlankWipe-1 12911
Sample Matrix
Notes/Comments: |
Analysis Requested
spores
spores
spores
spores
spores
^-••-^ y y
Relinquished By:
Date 1'ime:
Received Bv:
Date/Time:
C' A*-*-- / £**-~"S
^/3e?//47^^
//D/AtV^- ^^&^—
fi-3>-u #:/s"
Relinquished By:
Date Time:
Received By:
Date/Time:
ARCADIS U.S., Inc.
4915 Prospectus Drive, Suite F
Durham, North Carolina 27713
Phone Number: (919) 544-4535
Fax Number: (919)544-5690
-------
Serial Dilution/Plating Results Sheet
Test Information
Page 1 of 1
EPA Project No.
Technicians Name
Counters Name
2-14
Nicole Griffin Gatchalian
Nicole Griffin Gatchalian
PI
Test Date
Test No.
Paul Lemieux
11/29/2011-11/30/2011
Test3
Date Plated:
Date Counted:
11/29/2011
11/22/2011
Organism:
Volume Plated:
Gs
lOpjil
Temperature:
Extraction Volume:
55°C
20 mL
Tube Dilution
Plate
Sample ID Repl. 1.E+00 1.E-01 1.E-02 1.E-03 1.E-04 comments
VC-3
BS-3
Wipel-112911
Wipe2-1 12911
Wipe3-112911
Wipe4-1 12911
Blank Wipe-
112911
Rinsate- 3
ViaTrap Negative 3
ita\
-------
APPENDIX D: METROLOGY LAB CALIBRATION REPORTS
D-1
-------
US EPA APPCD Metrology Lab
Flow Rate Evaluation Report
Device Under Test
Mfr., Model
Serial Number
Met. Lab ID
Affiliation & PI
Requestor
Report File
MOP#
Bio Sampler #3
SKC, Bio-Sampler
5250
03661
NHSRC, DCMD, Paul Lemieux
Brent Hall
bio-sampler 03661 2010-12-17 qa.xls
Calibration Date
Location
Notebook, page
12/17/2010
High Bay
2273, p. 24
Ambient Conditions During Calibration
Temperature between 20 °C and 24 °C
RH between 5 % and 40 %
Pressure between 1,003 hPa and 1,008 hPa
Comments
Flow rate was evaluated at 2 different vacuum pressures within the devices operational range, with and without bio-sampler
fluid, and with 2 different pumps. All of the different conditions performed without any significant difference. Flow rates
under all the conditions averaged about 13.1 LPM.
Data and Results
Average
Stdev
Measured Flow Rate.
LPM
13.09
13.00
13.05
13.05
13.05
13.04
12.99
13.00
13.01
12.97
13.03
0.04
Target Flow Rate. LPM
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
0.00
Difference. LPM
0.59
0.50
0.55
0.55
0.55
0.54
0.49
0.50
0.51
0.47
0.53
0.04
Difference from Target was 4.2 %
Test Equipment
Device
Gilibrator 2 30 LPM cell
Hart 1560/2564 Thermistor Readout
Thermometrics Thermistor #5
Heise HQS-2, 0-15 PSIA #1
HeiseHQS-2, 0-15PSIA#2
Calibration Due
10/12/2011
3/19/2010
3/15/2011
1/13/2011
3/16/2011
SN
1010018-H
A35278
107
16760
30066
Uncertainty (2K)
±1.0% Reading
N/A
±0.051 °C
±0.19mmHg
±0.19mmHg
Evaluated by Mike Tufts
Page 1
-------
US EPA APPCD Metrology Lab
Flow Rate Evaluation Report
Device Under Test
Mfr., Model
Serial Number
Met. Lab ID
Affiliation & PI
Requestor
Report File
MOP#
Bio Sampler #4
SKC, Bio-Sampler
5276
03662
NHSRC, DCMD, Paul Lemieux
Brent Hall
bio-sampler 03662 2010-12-20 qa.xls
Calibration Date
Location
Notebook, page
12/20/2010
High Bay
2273, p. 25
Ambient Conditions During Calibration
Temperature between 20 °C and 24 °C
RH between 5 % and 40 %
Pressure between 1,005 hPa and 1,011 hPa
Comments
Flow rate was evaluated at 2 different vacuum pressures within the devices operational range with 2 different pumps. All of
the different conditions performed without any significant difference. Flow rates under all the conditions averaged about
13.0LPM.
Data and Results
Average
Stdev
Measured Flow Rate.
LPM
13.07
13.07
13.10
12.98
12.97
13.02
13.03
12.98
13.06
13.01
13.03
0.04
Target Flow Rate. LPM
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
12.50
0.00
Difference. LPM
0.57
0.57
0.60
0.48
0.47
0.52
0.53
0.48
0.56
0.51
0.53
0.04
Difference from Target was 4.2 %
Test Equipment
Device
Gilibrator 2 30 LPM cell
Hart 1560/2564 Thermistor Readout
Thermometrics Thermistor #5
Heise HQS-2, 0-15 PSIA #1
HeiseHQS-2, 0-15PSIA#2
Calibration Due
10/12/2011
3/19/2010
3/15/2011
1/13/2011
3/16/2011
SN
1010018-H
A35278
107
16760
30066
Uncertainty (2K)
±1.0% Reading
N/A
±0.051 °C
±0.19mmHg
±0.19mmHg
Evaluated by Mike Tufts
Page 1
-------
United States
Environmental Protection
Agency
PRESORTED STANDARD
POSTAGE & FEES PAID
EPA
PERMIT NO. G-35
Office of Research and Development (8101R)
Washington, DC 20460
Official Business
Penalty for Private Use
$300
------- |