United States       Prevention, Pesticides     EPA712-C-96-286
          Environmental Protection    and Toxic Substances     February 1996
          Agency         (7101)
&EPA    Biochemicals Test
          OPPTS 880.4425
          Dispenser Water

     This guideline is one  of a  series  of test  guidelines that have been
developed by the Office of Prevention, Pesticides and Toxic Substances,
United States Environmental  Protection Agency for use  in the testing of
pesticides and toxic substances, and the  development of test data that must
be submitted to the Agency  for review under Federal regulations.

     The Office of Prevention, Pesticides and Toxic Substances (OPPTS)
has  developed this guideline through  a process of harmonization that
blended the testing  guidance  and requirements that  existed in the Office
of Pollution Prevention and  Toxics  (OPPT) and appeared in Title  40,
Chapter I,  Subchapter R of the Code of Federal Regulations  (CFR),  the
Office of Pesticide Programs (OPP) which appeared in publications of the
National Technical  Information Service (NTIS) and the guidelines pub-
lished by the Organization  for Economic Cooperation and Development

     The purpose of harmonizing these  guidelines  into a single set of
OPPTS guidelines is to minimize  variations among the testing procedures
that must be performed to meet the data  requirements of the U. S. Environ-
mental Protection Agency  under  the Toxic  Substances  Control Act  (15
U.S.C. 2601) and the Federal Insecticide, Fungicide and Rodenticide Act
(7U.S.C. I36,etseq.).

     Final  Guideline Release: This guideline  is available from the U.S.
Government Printing Office, Washington, DC 20402 on The Federal Bul-
letin   Board.   By  modem  dial   202-512-1387,  telnet   and   ftp:
fedbbs.access.gpo.gov    (IP,    internet:     http://
fedbbs.access.gpo.gov, or call 202-512-0132 for disks  or paper copies.
This guideline is also available electronically in ASCII and PDF (portable
document format) from the EPA Public Access Gopher  (gopher.epa.gov)
under the heading "Environmental Test  Methods and Guidelines."

OPPTS 880.4425  Dispenser water leaching.
     (a) Scope—(1) Applicability. This guideline is intended to meet test-
ing requirements  of the Federal Insecticide,  Fungicide, and Rodenticide
Act (FIFRA) (7 U.S.C. 136, et seq.).

     (2) Background. The source material used in developing this har-
monized OPPTS test guideline is OPP guideline 155-5.

     (b) Leaching  of biochemical pesticides—(1)  Submission of  re-
quired data. Data on the leaching of a biochemical pesticide from a pas-
sive  dispenser to the environment  are required by 40 CFR 158.165 to sup-
port  the registration of every end-use product intended for outdoor applica-
tion  in such a dispenser and each manufacturing-use product which legally
may be used to formulate such an end-use product, whenever results of
any one or more of the tier I tests  specified in 40 CFR  158.690(d) indicate
potential adverse effects on nontarget organisms and the biochemical agent
is to be applied on land in a passive dispenser.

     (2) Test standards. In addition to the general test standards specified
in OPPTS 835.0001,  the following apply:

     (i) Test substance. Studies  shall be performed using the end-use
product when formulated for use in passive dispensers.

     (ii) Test procedure.  This  testing period should be eight hours, and
the leached pesticide should  be  extracted from the water in which the dis-
penser was soaked with a suitable organic solvent.  The method should
be that outlined in OPPTS 835.1230 and 835.1240.

     (c) Reporting and evaluation of data. The following provisions  for
reporting and evaluation apply:

     (1) Describe any significant deviations from the protocol. Report
the percentage  of biochemical  leached,  the percentage  remaining  in  the
dispensers, and the percentage unaccounted for.

     (2) [Reserved]

     (d) Details of method. The following is an example of an acceptable
protocol for measuring leaching of the agent from the dispenser into water:

     Example:  Place a known amount  of the  formulated pesticide in
unstirred water in a  wide-mouth container. The water volume should be
large enough so  that the  water solubility of active ingredients  will not
be exceeded if all of the active ingredients were to leach out of the dis-
pensing device. Allow the pesticide/water mixture to stand for eight hours.
Filter off  or remove the pesticide dispensers and  extract the water with
hexane, or some other appropriate volatile  organic solvent. Dry the extract
and determine the amount of semiochemicals either by direct measurement
or after separation or concentration. At the same time, extract the dispens-

ers that have been removed from the water, using an appropriate organic
solvent. Sample this second extract directly or after a separation or con-
centration step,  and determine the  amount  of semiochemicals that have
not leached.  Calculate the percent  of semiochemicals that leached from
the dispenser.