United States Prevention, Pesticides EPA712-C-96-286
Environmental Protection and Toxic Substances February 1996
Agency (7101)
&EPA Biochemicals Test
Guidelines
OPPTS 880.4425
Dispenser Water
Leaching
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INTRODUCTION
This guideline is one of a series of test guidelines that have been
developed by the Office of Prevention, Pesticides and Toxic Substances,
United States Environmental Protection Agency for use in the testing of
pesticides and toxic substances, and the development of test data that must
be submitted to the Agency for review under Federal regulations.
The Office of Prevention, Pesticides and Toxic Substances (OPPTS)
has developed this guideline through a process of harmonization that
blended the testing guidance and requirements that existed in the Office
of Pollution Prevention and Toxics (OPPT) and appeared in Title 40,
Chapter I, Subchapter R of the Code of Federal Regulations (CFR), the
Office of Pesticide Programs (OPP) which appeared in publications of the
National Technical Information Service (NTIS) and the guidelines pub-
lished by the Organization for Economic Cooperation and Development
(OECD).
The purpose of harmonizing these guidelines into a single set of
OPPTS guidelines is to minimize variations among the testing procedures
that must be performed to meet the data requirements of the U. S. Environ-
mental Protection Agency under the Toxic Substances Control Act (15
U.S.C. 2601) and the Federal Insecticide, Fungicide and Rodenticide Act
(7U.S.C. I36,etseq.).
Final Guideline Release: This guideline is available from the U.S.
Government Printing Office, Washington, DC 20402 on The Federal Bul-
letin Board. By modem dial 202-512-1387, telnet and ftp:
fedbbs.access.gpo.gov (IP 162.140.64.19), internet: http://
fedbbs.access.gpo.gov, or call 202-512-0132 for disks or paper copies.
This guideline is also available electronically in ASCII and PDF (portable
document format) from the EPA Public Access Gopher (gopher.epa.gov)
under the heading "Environmental Test Methods and Guidelines."
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OPPTS 880.4425 Dispenser water leaching.
(a) Scope—(1) Applicability. This guideline is intended to meet test-
ing requirements of the Federal Insecticide, Fungicide, and Rodenticide
Act (FIFRA) (7 U.S.C. 136, et seq.).
(2) Background. The source material used in developing this har-
monized OPPTS test guideline is OPP guideline 155-5.
(b) Leaching of biochemical pesticides—(1) Submission of re-
quired data. Data on the leaching of a biochemical pesticide from a pas-
sive dispenser to the environment are required by 40 CFR 158.165 to sup-
port the registration of every end-use product intended for outdoor applica-
tion in such a dispenser and each manufacturing-use product which legally
may be used to formulate such an end-use product, whenever results of
any one or more of the tier I tests specified in 40 CFR 158.690(d) indicate
potential adverse effects on nontarget organisms and the biochemical agent
is to be applied on land in a passive dispenser.
(2) Test standards. In addition to the general test standards specified
in OPPTS 835.0001, the following apply:
(i) Test substance. Studies shall be performed using the end-use
product when formulated for use in passive dispensers.
(ii) Test procedure. This testing period should be eight hours, and
the leached pesticide should be extracted from the water in which the dis-
penser was soaked with a suitable organic solvent. The method should
be that outlined in OPPTS 835.1230 and 835.1240.
(c) Reporting and evaluation of data. The following provisions for
reporting and evaluation apply:
(1) Describe any significant deviations from the protocol. Report
the percentage of biochemical leached, the percentage remaining in the
dispensers, and the percentage unaccounted for.
(2) [Reserved]
(d) Details of method. The following is an example of an acceptable
protocol for measuring leaching of the agent from the dispenser into water:
Example: Place a known amount of the formulated pesticide in
unstirred water in a wide-mouth container. The water volume should be
large enough so that the water solubility of active ingredients will not
be exceeded if all of the active ingredients were to leach out of the dis-
pensing device. Allow the pesticide/water mixture to stand for eight hours.
Filter off or remove the pesticide dispensers and extract the water with
hexane, or some other appropriate volatile organic solvent. Dry the extract
and determine the amount of semiochemicals either by direct measurement
or after separation or concentration. At the same time, extract the dispens-
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ers that have been removed from the water, using an appropriate organic
solvent. Sample this second extract directly or after a separation or con-
centration step, and determine the amount of semiochemicals that have
not leached. Calculate the percent of semiochemicals that leached from
the dispenser.
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