PA-SAB-WKS-05-001
                           June 2005
                       www.epa.gov/sab
       Nanotechnology,
     Biotechnology, and
  Information Technology:
   Implications for Future
       Science at EPA
A Workshop of the EPA Science Advisory Board

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                        TABLE OF CONTENTS


1.0 WORKSHOP BACKGROUND AND OBJECTIVES 	  1

2.0 WORKSHOP OVERVIEW	  1

3.0 KEY WORKSHOP FINDINGS AND CROSS CUTTING
RECOMMNEDATIONS	  2

4.0 KEYNOTE ADDRESS SUMMARY	  5

      4.1 Industrial Ecology Principles: A Unifying Theme For
      Environmental Applications of New Technologies -
      Dr. Braden Allenby, School of Engineering, Arizona State
      University	  5

5.0 NANOTECHNOLOGY	  7

      5.1 Invited Presentation: Nanotechnology - Dr. Roland Clift,
      Centre for Environmental Strategy, University of Surrey	  7

      5.2Nanotechnology Breakout Group Report	  8

6.0 BIOTECHNOLOGY - BIOPROCESSING	  9

      6.1 Invited Presentation: Bioprocessing:  Opportunities and
       Challenges - Dr. HaroldMonbouqette, University of California
       - Los Angeles	  9

      6.2 Bioprocessing Breakout Group Report	  10

7.0 BIOTECHNOLOGY - GENOMICS	  13

      7.1 Invited Presentation: Towards Genomics-based Analyses of
      Environmental Agent Impacts on Biological Genomics
      Dr. Bruce Aronow, Cincinnati Children's Hospital  13

      7.2 -Omics Sciences Breakout Group Report	  14

8.0 INFORMATION TECHNOLOGY - SENSOR NETWORKS	  15

      8.1 Invited Presentation: Wireless Sensor Networks for Environmental
      Monitoring - Dr. Deborah Estrin, University of California - Los Angeles....  15

      8.2 Sensor Networks Breakout Group Summary Report	  16
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9.0 INFORMATION TECHNOLOGY - LARGE SCALE COMPUTING	  18

       9.1 Invited Presentation: Information Technology (IT):  Implications for
       Future Science at EPA - Dr. Gregory McRae, Massachusetts Institute of
       Technology 18

       9.2 Large Computing Breakout Group Report	  20
10.0 CONVERGING TECHNOLOGIES	  21

       10.1 Invited Presentation: Converging Technologies -Dr. William S.
       Bainbridge, National Science Foundation	 21

       10.2 Converging Technologies Break out Group Report  	 23
                           LIST OF APPENDICES
Appendix A - Agenda

Appendix B - Biosketches of Invited Speakers and Subject Matter Experts

Appendix C - Breakout Group Assignments

Appendix D - Breakout Group Discussion Questions

Appendix E - Slide Presentation - Environment for the 21st Century - Industrial Ecology
Principles: A Unifying Theme for Environmental Applications of New Technologies - Dr.
Braden Allenby

Appendix F - Slide Presentation - Nanotechnology - Dr. Roland Clift

Appendix G - Slide Presentation - Bioprocessing: Opportunities and Challenges -
Dr. Harold G. Monbouquette

Appendix H - Slide Presentation - Towards Genomics-basedAnalyses of
Environmental Impact on Biological Systems - Dr. Bruce Aronow

Appendix I - Slide Presentation - Wireless Sensor Networks for Environmental
Monitoring - Dr. Deborah Estrin

Appendix J - Slide Presentation - Information Technology (IT): Implications for Future
Science at EPA - Dr. Gregory McRae

Appendix K - Slide Presentation - Converging Technologies - Dr. William Bainbridge
                                      in

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NANOTECHNOLOGY, BIOTECHNOLOGY, AND INFORMATION
TECHNOLOGY: IMPLICATIONS FOR FUTURE SCIENCE AT EPA


1.0 WORKSHOP BACKGROUND AND OBJECTIVES
       The U.  S. Environmental Protection Agency  (EPA  or the Agency) science
programs  have  primarily  focused  on  characterizing  and  managing  risk  from
environmental exposure to chemical  and biological, and physical  stressors.  Much of the
Agency's  ongoing work is based on  managing historical sources of  pollution.   The
Agency will continue working on these legacy problems, but it also faces opportunities
and challenges  from emerging  technologies, products and services.   EPA research
programs currently look toward emerging environmental issues.  However, science and
technology continues to expand at unprecedented rates. This expansion has been referred
to as a new industrial and economic revolution.  It offers new  opportunities, but also
brings unanswered questions about their potential environmental risks and benefits.  The
present science and technology expansion coincides with flat to  declining EPA science
budgets for the foreseeable future.  Accordingly, the Agency is faced with resolving
existing environmental problems and developing new strategies for emerging concerns.

       The EPA Science Advisory Board (SAB or the Board) has  urged the Agency to
develop a new science vision for  human health and environmental  protection that
incorporates the latest scientific  and technological advancements.   Developments and
emerging applications in Nanotechnology, Biotechnology and Information Technology
over the past decade have been dramatic, and will continue into the foreseeable future.
Advancements within and between these  and  other  technologies will revolutionize
industrial production and economic expansion, as well as the environmental sciences.

       The SAB anticipates that as the Agency mission becomes more involved with
Nanotechnology, Biotechnology and Information Technology products and services, the
Board will be asked to provide advice to the Administrator on EPA science and research
needs in these area.  The primary objective of this workshop was to educate and inform
the SAB, and to initiate a dialogue on the implications of these technologies for science
and research advice to the EPA.
2.0 WORKSHOP OVERVIEW
      Workshop participants included members of the  SAB, the Clean Air Scientific
Advisory  Committee  (CASAC),  the Advisory Council  on Clean  Air  Compliance
Analysis (COUNCIL), and their committees.  The workshop (Agenda in Appendix A)
included invited presentations  on Industrial Ecology,  Nanotechnology; Bioproduction;
Genomics;  Sensor Networks;  Large  Scale  Computing Applications; and Converging
Technologies.  Industrial Ecology was selected as a potential unifying theme for the six
subsequent technology subjects.

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Following  the invited  presentations,  the speakers,  invited  subject  matter  experts
(Biosketches  in Appendix B),  and workshop participants met in six breakout groups
(Breakout Group Assignments in Appendix C) corresponding to the six technology areas.
Following breakout group discussions (Breakout Group Questions in Appendix D) the
workshop participants discussed the break out group results in plenary session.

       This document summarizes:  key findings and cross-cutting recommendations of
the workshop(Section 3.0);  the Industrial Ecology keynote address (Section 4.0 and
Appendix E ); and invited presentations and breakout group  reports for Nanotechnology
(Section 5.0 and Appendix F), Biotechnology - Bioprocessing (Section 6.0 and Appendix
G), Biotechnology - Genomics (Section 7.0 and Appendix H), Information Technology -
Sensor Networks  (Section 8.0 and Appendix 7),  Information Technology - Large  Scale
Computing (Section 9.0 and Appendix J),  and Converging Technologies (Section 10 and
Appendix K).

3.0 KEY WORKSHOP FINDINGS & CROSS CUTTING RECOMMENDATIONS
       The workshop demonstrated that advances and applications within and between
Nanotechnology, Bioproduction, Genomics, Sensor Networks, Large Scale Computing,
and Converging   Technologies  are  occurring at  an  unprecedented rate.    Such
advancements offer substantial  opportunities  and  challenges for  EPA  science and
research  planning and implementation and are provided in  subsequent sections of this
report. Further, they will undoubtedly impact the work of the Agency now and into the
future. Key workshop findings and cross-cutting issues raised during the workshop are
provided  first, followed  by  the  findings  and   recommendations  for  the specific
technologies.


          •   Industrial ecology can be used as  an evaluation framework for the
              development,   application,   commercialization,   dispersal  and
              potential environmental opportunities and challenges of materials
              and products resulting from new technologies.


          •   Industrial ecology  can assist a  shift from  simple control and
              engineering  solutions  for product  artifact manufacture  and
              disposal;  to  evaluation   of  complex  adaptive   systems  that
              incorporate real time adjustment and dialogue to address broader
              cultural impact of services.


          •   A strategic examination of how industrial ecology might integrate
              new technologies to assist the Agency in setting priorities for  its
              most pressing problems may be warranted.


          •   Possible applications of nanotechnology and nanomaterials include
              their use in batteries and fuel cells, smart packaging and labeling,
              catalysts  and  separation  membranes,  paints   and  coatings,

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   lubricants,  composites,  medical  diagnosis  and  drug  delivery
   systems, and self-replicating robots and assemblers.


•  Aspects   of nanotechnology  and  nanomaterials  that  require
   additional  investigations include quantifiable estimates of their
   benefits, and their environmental, health and social impacts.


•  Bioprocessing and the biorefmery concept can be used to produce
   a  broad  spectrum of  products from engineered plants  (e.g.,
   starches, sugars, proteins, fibers, fuels, oils, antibodies and drugs)
   using economical, and environmentally friendly processes.


•  Bioprocessing challenges include determining the environmental
   impact of biorefmeries; bioprocess design; limited knowledge of
   metabolism   and   control   mechanisms;    and  health   and
   environmental effects of new classes of products.


•  As  an   emerging  science,   -omics   technologies  (genomics,
   proteomics,  metabolomics,  etc.) offers significant  potential to
   improve and refine EPA's mission of protecting human health and
   the environment.


•  Obstacles to -omics based health and  environmental  applications
   include:

       o  The lack of quantitative methods, full genomic sequences,
          reference and technical standards, and notation datasets for
          at-risk populations; and

       o  Limited   knowledge   of  cross-species   generalizations;
          environmental   generalizations,   genes   that   confer
          environmental agent sensitivity, normal states,  and damage
          indices.


•  Embedded  sensor  networks  include  micro  sensors,  onboard
   processing,  and wireless interfaces at very small scale that enable
   spatially  and temporally  dense  environmental monitoring of
   previously unobservable phenomena


•  Important challenges  for sensor network applications include the
   development of  sensors, platforms,  software protocols,  energy

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   awareness  and conservation, scaling and adaptation to  variable
   resources and stimuli.
•  Large-scale  Computer  applications  can reduce  the  time for
   applying science in environmental decision-making, and have the
   potential to revolutionize how EPA might manage environmental
   decisions in diverse areas including:


•  Computational  chemistry  and toxicology, modeling, monitoring,
   real  time emergency response,  econometrics; decision analysis;
   optimized  economic  growth; urban  system management;   and
   scenario  analysis  for  economic,  social  and  environmentally
   sustainable solutions under uncertainty.


•  Potential environmental applications for converging technologies
   range from wearable sensors  and computers to enhance awareness
   of health,  environment,  potential  hazards,  natural  resources; to
   environmental networks  of cheap,  smart sensors that  constantly
   monitor the condition of the environment.


•  Advancements in new technology  are occurring at unprecedented
   rates, making it difficult for government agencies to keep abreast
   of:
          - emerging developments;

          - science and technology skill mix needs;

          - priorities of new technologies against existing
            research strategies and multi year plans;

          - collaboration and interaction  with other governments,
          federal agencies, science advisory committees, industry,
          academia, and the public.


•  Emerging technologies can resolve complex  environmental and
   energy   problems  with   multiple  and  conflicting  objectives,
   asymmetric information, short decision cycles, long analysis times,
   and few technically qualified people.


•  Emerging  technology  development  and  applications  require
   consideration and  integration of social  sciences  (economics,

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              decision  sciences,  etc.)  to  determine  potential  environmental
              benefits and impacts.


          •   The range of applications offered by new technologies that would
              benefit EPA's science and research activities is vast, and priorities
              should be strategically  targeted to  address  the  Agency  most
              pressing priorities.


          •   Conventional toxicological and risk assessment approaches  have
              largely been developed for chemicals and require modification for
              nanotechnology, biotechnology, and sensor deployment.


          •   The SAB might consider workshops that focus on strategic issues
              associated with the  development  and  deployment  of  new
              technologies, as well as targeted workshops on novel applications
              of specific technologies for specific problems.


          •   The SAB might consider additional  committees to address the
              development of new technologies.

       In the closing discussion, participants noted that technology appears to be on the
verge of altering the entire context of environmental protection and social welfare.  Some
questioned  whether  conventional  risk  assessment  and  regulatory   structures  are
appropriate for the potential environmental challenges of emerging technologies.  Others
asked whether consideration needed to be given to altering our institutional  structures to
meet these challenges. Still others thought that  the SAB might provide deeper thinking
about where the EPA needs to be in ten years, and what it needs to do to get there.

        In closing remarks Dr. Granger Morgan, Chair of the SAB, noted that the topics
and recommendations emerging from the workshop were diverse.  He observed that
specific recommendations were valuable, but that real impact of the workshop was a
diffusion process.  That is, workshop participants take what they have learned back to
their offices, and incorporate new thinking into their work.  For the SAB, this means new
ideas in reviewing EPA projects, programs, planning documents, and the science budget.
He stated that the ideas discussed  at this workshop will set the stage for additional SAB
deliberations regarding how to best advise the Agency on the use of new technologies in
its science enterprise.


4.0 KEYNOTE ADDRESS SUMMARY
4.1    Industrial Ecology Principles: A Unifying Theme for Environmental
       Applications of New Technologies — Dr. Braden Allenby, School of
       Engineering, Arizona State University (See Slides Appendix E)

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       Industrial Ecology is a systems approach to environmental analysis.  It addresses
industrial emissions,  specific products, and the complex network of services, products
and activities that make up the economy.  Industrial ecology can guide holistic thinking
about environmental problems.  The need for guiding principles is illustrated by the rapid
societal changes being brought  about by advancements in  technology.   Technology
causes fundamental changes,  and the rate  of change  currently  exceeds the  ability of
governments  to  react in  a timely  manner.  Therefore,  timely  decisions and specific
courses of action are preempted by rapid societal change, which often lacks systematic
consideration of holistic environmental consequences.  Dr. Allenby discussed two case
studies that demonstrated the need to shift environmental analysis and problem-solving
away from simple command  and control  of product manufacture and  emissions, and
towards complex adaptive systems.   Several  key points follow,  and Dr.  Allenby's
complete slide presentation is shown in Appendix E.

          •  Traditional  environmental  science  and  engineering  has  been
             directed  toward   controlling physical   environmental  impacts
             associated with energy consumption and toxic products related to
             artifact manufacture and disposal.


          •  Although  less  intuitive  and   much   less   studied,  cultural
             environmental impacts  from such  services may be  potentially
             large.  Critical thinking is required to resolve ethics, fundamental
             changes in human cognition and perception through computers and
             information technology.


          •  Industrial ecology principles (earth systems  engineering and
             management related to design engineering, governance and theory)
             provide a unifying theme for environmental applications of new
             technologies.


          •  Bothersome Questions for the SAB to consider

                    - Should EPA have a Technology and Science Advisory
                    Board, and should  an Industry  Advisory Board be
                    added?

                    - Should EPA become a competency that diffuses itself
                    throughout government?

                    - How will  EPA and government generally, develop the
                    ability to  engage  ialogue with,  rather than regulate,
                    complex human/natural systems?

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                    - How will EPA develop the ability to operate on a time
                    cycle that aligns with the phenomenon for which it is
                    responsible?

                    - How  will  EPA  function  as its  core conceptual
                    foundations (environment,  wilderness, nature) become
                    increasingly contingent, and change substantively over
                    shorter time periods?

                    - What is EPA's role as the world increasingly becomes
                    a product of human design?

                    - How  does  EPA  avoid  becoming  more  and more
                    effective,  at  less   and   less  important  tasks,  as
                    environmental impacts increasingly become a function
                    of strategic and  non-environmental technological  and
                    business decisions?
5.0 NANOTECHNOLOGY

5.1    Invited  Presentation:  Nanotechnology  — Dr.  Roland  Clift,  Centre  for
       Environmental Strategy, University of Surrey (See Slides Appendix F).

       Nanotechnology is an emerging technology based on solid particles in the  size
   range of 1-100 nm (a  nanometer is IX 10"9 meter and comparable in size to viruses)
   where properties are determined by size and surface area  rather than bulk properties.
   A member of the Royal Society/Royal Academy of Engineering Working Group on
   Nanoscience and Nanotechnologies, Dr. Clift provided a European perspective based
   the Working Group report Nanoscience  and Nanotechnologies:  Opportunities  and
    Uncertainties  (2004).   He  discussed possible applications including the  use of
   nanomaterials  in batteries and fuel cells,  smart packaging and labeling, catalysts and
   separation  membranes,  paints and coatings, lubricants,  composites, and  medical
   diagnosis and  drug delivery systems, and self-replicating  robots and assemblers.  He
   focused his remarks on three areas of concern:  quantifiable benefit estimates; health
   and environmental impacts;  and social impacts of new and emerging technologies.
   Several key points follow, and Dr. Cliffs complete slide presentation is shown in
   Appendix F.

          •  Systematic life cycle assessments  of the benefits  and risks of
             nanotechnology have not yet been conducted,  the potential health
             and environmental impacts are uncertain, and social impacts are
             unknown.

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          •  Conventional hazard and risk endpoints may provide a basis for
             regulation,  but   standard   tests   (e.g.,   toxicity,  persistence,
             bioaccumulation) may not be applicable  for nanomaterials due to
             surface property alterations at the nanoscale.


          •  Toxicity information is lacking, and regulation must be based on
             likely nanoparticle exposure scenarios (e.g., vehicle emissions, sun
             screens, cosmetics, and combustion).

          •  The precautionary  approach suggests a  moratorium on  certain
             nanotechnology applications (e.g., fuel additives, bioremediation
             of  groundwater,  and  end-of-life product disposal).   However,
             nanoparticles  are  likely to  be made at point of use, making
             arguments for a production moratorium irrelevant.


          •  The Royal Society/Royal  Academy  of Engineering  Working
             Group recommended that  Europe  conduct horizon scanning of
             emerging technologies  by  asking  what impacts and  regulatory
             issues might arise.
5.2    Nanotechnology Breakout Group Report
   The breakout group participants  (Appendix C)  discussed current government and
industrial  initiatives and projects, applications, and possible  risk assessment and risk
management issues regarding nanomaterials.  The following points were prepared for the
plenary presentaion and discussion.

   Basics
          •  Nano-size and nano-materials have to be considered together.


          •  Life cycle assessments should consider what is being made, where
             it goes,  and where it ends up?


          •  Presently, over 200 companies worldwide are involved in making
             nanoproducts.


          •  Nanotechnology development may be going the way of uncritical
             praise and optimism, and there may be lessons to be learned from
             earlier  controversies  (e.g.,  genetically  modified  organisms  and
             nuclear power).

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   Opportunities

          •  Nanotechnology application holds great promise  for  electricity
             transmission, solar conversion, catalysis, sensors,  treatment and
             purification technologies, and   the  remediation   of  hazardous
             wastes.
   Challenges

          •  Public discussion  on  nanotechnology should be encouraged and
             amplified.

          •   Life  cycle  assessment  should be  applied to nanomanufacturing
             and nanoproduct footprints.

          •  Standards  and  measurements  (testing protocols)  need  to be
             developed for nanotechnology research.

   Future Role of SAB

          •  SAB  can help  identify  the most urgent environmental problems
             (SAB   nanotechnology  review,   need   for   environmental
             nanotechnology science  plan, etc).

          •  Should SAB help EPA reconsider  its relationship  to industry
             (Being informed enough to know what will  be happening; doing
             collaborative research)?
6.0 BIOTECHNOLOGY - BIOPROCESSING

6.1     Invited Presentation:  Bioprocessing:  Opportunities and Challenges — Dr.
       Harold Monbouqette,  University  of California  - Los  Angeles  (See  Slides
       Appendix G)

       Bioprocessing  exploits  a broad universe of metabolic processes  and enzyme
    activities to synthesize specialty and commodity chemicals. The biorefinery concept
    is closely associated with bioprocessing, but provides a commodities  development
    perspective.   Presently, there is a diverse enzyme toolkit available to industry.  Of
    approximately 30,000 known enzymes, about 3000 have been well characterized, and
    about 300 are commercially available.  Accordingly, available  techniques allow
    engineering  of plants, microbes and enzyme systems for production of chemicals
    using economical, and environmentally friendly processes.  Bioprocessing can be
    used to  produce a broad spectrum of products from engineered organisms, including
    starches, sugars, proteins, fibers, fuels, oils, antibodies and drugs.  Dr. Monbouquette

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   focused on several applications in his area of expertise.  Several key points follow,
   and his complete slide presentation is shown in Appendix G.

          •  Bioprocessing  and  the biorefinery  concept  exploit metabolic
             processes and enzyme activities for the production of specialty and
             commodity chemicals.

          •  Examples include:  production of carotenoid pigments from genes
             cloned into E. coli; biosynthesis pathways for aspartame, melanin,
             and indigo; and the integration of enzymes into chemical synthesis
             processes to reduce environmental impacts.

          •  Bioprocessing has the potential to provide new products including
             chiral  drugs,  flavorings,  aromas,   herbicides  and  pesticides,
             hyperthermophilic  glycoside  hydrolases  for   oil  and gas well
             fracturing. New systems may be needed to assess environmental
             impact of these processes and  products including,  for example,
             methods for detecting  potential endocrine disrupting chemicals
             (EDCs).

          •  Bioprocessing  presents  several opportunities  beneficial for the
             environment including:    genetically modified  organisms  to
             synthesize chemicals from renewable resources; and enzymes to
             improve selectivity and yield of industrial chemical synthesis steps
             thereby reducing environmental impact.

          •  Challenges  presented by bioprocessing include determining the
             environmental  impact of biorefineries; bioprocess design; limited
             knowledge of metabolism and metabolic control mechanisms; and
             health and environmental effects of new classes of  products and
             processes.
6.2    Bioprocessing Breakout Group Report
       The breakout group participants (Appendix C) discussed potential bioprocessing
applications,  as well as risk assessment,  risk  management,  and  policy  needs.  The
following points were prepared for the plenary presentation and discussion.

 Opportunities

          •  Switching from a  petroleum-based  economy  to  bioproduction
             provides opportunities to reduce the toxicity of industrial waste and
             byproducts.

          •  Bioproduction  offers opportunities to use agricultural products and
             waste materials (e.g., agricultural waste) in fermentation processes.
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•  Small community-based systems are important to allow innovations
   in bioproduction at the local scale.

•  Metabolic engineering using recombinant DNA technology has the
   potential to improve production of chemicals by host organisms, and
   allow production of new chemicals.

•  Currently, some biomass (e.g., cellulose) cannot be effectively used
   in   conventional   bioproduction.   Gasification,   followed  by
   biosynthesis provides a near term opportunity for effective use of
   cellulitic biomass as raw materials in production processes.

•  Technology is being developed to use biomass such as grass, wood
   and waste material in bioproduction processes.

•  Bioproduction offers  opportunities for animal waste reduction and
   more efficient use of nutrients (e.g., phosphorus fed to chickens).

•  In the  near term,  advancements in viable, environmentally safe
   technology should be a priority (e.g., use of systems in landfills to
   remove methane).

•  How EPA might regulate the use of new technologies is a key issue.
   Science needs and regulatory impediments should be addressed for
   regulating genetically modified organisms used in and products from
   bioproduction.

•  EPA should consider incentives to advance the state of the science
   through innovative approaches like credit trading programs for waste
   generators.

•  EPA needs to catalyze formation of university/industry/other federal
   agency partnerships to conduct innovative research and development
   and more effective integration of bio-based green chemistry work.

•  EPA should  articulate research needs and provide  more  external
   support for research and training of graduate students in emerging
   areas.

•  EPA should develop  multidisciplinary  approaches  for  life cycle
   analysis.  To encourage innovation, a framework not a standard
   protocol is needed.
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Challenges

       •  Environmental   problems   associated   with   more   intensive
          agricultural production in different crops must be considered.

       •  Energy and fertilizer demands in agriculture are high.

       •  Degradation of soil, displacement of wildlife, and water quality
          problems (hypoxia caused by nitrogen and phosphorus runoff from
          farm fields) must be considered.

       •  Tradeoffs between  environmental  benefits of bioproduction and
          benefits  of  reducing the  intensity  of agriculture  (e.g., taking
          marginal land out  of production, converting land to wetlands)
          should be evaluated.

       •  Research is  needed to understand  ripple  effects  of bioproduction
          through land use and  social and economic systems (these may be
          very large).

       •  Potential  environmental   effects   of  accidental   releases  of
          recombinant DNA must be considered.

       •  Biosafety guidelines are needed for bioproduction technologies.

       •  Studies of genetically modified  organisms used  in more  open
          processes such as biorefineries should be  conducted to quantify
          environmental benefits and evaluate benefits versus risks.

       •  Regulatory authority for  genetically modified organisms between
          agencies should be clarified.

       •  New   toxicology  tools   should  be  developed   to  examine
          bioproduction.

       •  EPA should develop good  management practices for testing new
          technologies to determine whether they may cause environmental
          problems.

       •  Good  sensors  are  needed to  conduct  assessments of  new
          technologies.

Future Role of the SAB

       •  SAB should continue  to hold technical workshops like this one in
          order to anticipate emerging issues.
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          •  SAB  should encourage EPA  to  work closely  with the science
             advisory committees  of other government agencies, particularly
             DOE, USD A, and Commerce on bioproduction issues.

7.0 BIOTECHNOLOGY - GENOMICS

7.1    Invited  Presentation:   Towards genomics-based analyses  of environmental
       agent impacts on biological systems - Dr. Bruce Aronow, Cincinnati Childrens
       Hospital (See Slides Appendix H)

    The term genomics specifically refers  to the study of the structure, activity and
functions of genes. It includes gene  regulation, mRNA expression, and cell-type
specificity. Genomics is often imprecisely used to cover other "-omics" sciences
such as physiomics (tissue dynamics, systems biology, and the outcome in clinical
populations)  and  proteomics   (protein  expression,  structure,  interactions,
localizations and pathways).   Dr. Aronow focused his presentation on using
genomics to assess environmental effects  on biological systems, with emphasis on
mouse and human models for colon cancer.  Several key points follow, and his
complete slide presentation is shown in Appendix H.


          •  Genomics can provide new tools to assess the impact of
             environmental agents.

          •  Systems biology approaches will assist the integration of genomics
             data and analyses into  human health and environmental assessment
             scenarios.

          •  Technical barriers currently present obstacles to genomics-based
             health and environmental monitoring. These include:

                 -  Lack of quantitative methods, full genomic sequences,
                    reference and technical standards, and notation datasets
                    for at-risk populations;

                    Limited knowledge of cross-species and environmental
                    generalization, genes that confer environmental agent
                    sensitivity, normal  states, and damage indices.

          •  Two case studies were presented using human and mouse central
             nervous system genes; and comparative transcriptional profiling
             for mouse and human colon cancers.
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                    The case studies demonstrated the classification of
                    human tumors based on behavior of developmentally
                    regulated mouse gene orthologs that have implications
                    for outcomes to individuals.
7.2    -Omic Sciences Breakout Group Report
       The breakout  group participants  (Appendix  C) discussed potential genomic
applications, as  well  as risk  assessment, risk management, and  policy  needs.  The
following points were prepared for the plenary discussion.

   Opportunities

          •  -Omics  technology  is  not limited  to genomics, but  includes
             proteomics, metabolomics, etc.

          •  As an  emerging science,  -omics technology offers significant
             potential to improve and refine EPA's mission of protecting human
             health and the environment.

          •  Complexity,  costs,  and   effective  implementation  of  -omic
             technology demands new models of research partnerships,  both
             within and across federal  agencies, and with  external  research
             communities  and sectors.

          •  Engage ongoing efforts in NAS,  OECD and others developing
             application plans for biotechnology.

          •  -Omics  technology has value for EPA's mission (e.g., identifying
             susceptible   populations,   surveillance  analysis,  prioritization,
             reduced use of animals fro testing).

   Challenges

          •  EPA  should  develop a Framework (Multi-Year Plan) focused on
             implementation  of  -omics technology  that  covers:  partnerships;
             attraction,   retention,   and  training   of   human   resources;
             bioinformatic needs and integration with other databases; systems
             biology  and integrated  modeling  capacity;  development of
             performance  standards;  commonality of methods; consistency of
             performance/baseline measurements; external data submission; and
             training sets for interpretation.

          •  The Multi-Year Plan research plan should be developed in keeping
             with OMBs Program Assessment Review Tool (PART).
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   Future Role of SAB
          •  Consider interactions with other agency Science Advisory Boards,
             and examine new models of cross-agency funding and resource
             sharing, funding needs, ethics, and the value of -omics technology
             to key customers.

          •  Consider priorities of -omics technologies relative to other multi-
             year plans.
8.0 INFORMATION TECHNOLOGY - SENSOR NETWORKS

8.1    Invited Presentation: Wireless Sensor Networks for Environmental
       Monitoring — Dr. Deborah Estrin, University of California - Los Angeles (See
       Slides Appendix I)

    Embedded sensor networks include micro sensors, onboard processing, and wireless
interfaces at very  small  scale to monitor phenomena up-close; enable spatially and
temporally dense  environmental  monitoring;  and  reveal  previously unobservable
phenomena. Dr. Estrin focused her presentation on ecological and contaminant transport
applications, as well as regional and global possibilities for sensor network development.
Several key points follow, and her complete slide presentation is shown in Appendix I.


          •  The emerging technologies  discussed  in  this  workshop  offer
             opportunities for development  of new sensor networks to observe,
             monitor and model various functions.

          •  The specific embedded sensor networks applications discussed
             included contaminant  transport  in soils,  plankton dynamics  in
             marine environments, and ecosystem processes.

          •  In situ  Sensing will transform observations of spatially  variable
             processes in heterogeneous and obstructed environments. Example
             applications include a locally dense surface and subsurface sensor
             network to  observe soil nitrate transport;  spatial and temporal
             distributions  of algal blooms  in  coastal ecosystems;  ecosystem
             processes such as microclimate  monitoring, image and  acoustic
             sensing, and infrastructure mobility.

          •  Important  challenges  for sensor  network  applications  include
             sensors,  platforms,  software  protocols,  energy  awareness and
             conservation,  scaling and adaptation to variable  resources and
             stimuli.
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          •   Heterogeneous sensor networks of small linked robotic sensors that
              host higher-end sensors are needed to enable adaptive, fidelity-
              driven, three-dimensional sampling.

          •   The development  of embeddable sensor networks and multi-scale
              observation and fusion networks  have broad relevance to global
              issues.
8.2    Sensor Networks Breakout Group Report
   The breakout group participants (Appendix C) discussed the rapid advancement of
sensor network technology,  and possibilities for cheap, small sensors capable of multi-
factor  analysis,  data-relay, and network  integration.  Future applications might well
include laboratories on a chip, and mass spectrometers the size  of sugar cubes.  The
breakout group focused their attention on new technologies, demonstration projects, and
prepared a set of network development technology principles, challenges and future role
of the SAB for presentation and discussion at the plenary session.

 Principles for Sensor Network Development

          •   Affordability (The National Ambient  Air Monitoring Strategy is
              one approach for redesign within existing annual total costs
              (http://www.epa.gOv/ttn/amtic/files/ambient/monitorstrat/allstrat.p
              df).

          •   Problem-oriented applications  focused on solving  environmental
              problems of critical importance to EPA's mission and regulatory
              mandate.

          •   Technologies that are realistically  and demonstrable in the near-
              term

          •   Partnerships should be developed to:

                    Ensure commercial viability of new technologies to
                    capitalize on corporate investments made by industry;

                    Utilize existing  data networks in the federal,  state,
                    tribal, and local sectors;

                    Partner with  other  government agencies  engaged in
                    basic and applied sensor-network research to leverage
                    research funding and capabilities.
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         •  Focus on multi-use, multi-pollutant sensors  and applications that
            include  sensors  for  ecological,  biological  and  human  health
            applications.

         •  Specify interoperability and comparability of data by design and
            select data networks and  embedded sensors that are interoperable
            with analytical data equivalence.

         •  Employ   multi-layer,  large  to   small   scale  sensor  networks
            (e.g.,satellite  imagery and  smaller-scale  remote-sensing to local
            on-site sensors).

         •  Ensure data are easily interpretable and include data visualization
            techniques (i.e., visual display of dense,  complex quantitative and
            qualitative information  from embedded sensor networks), leading
            to clear unambiguous interpretation.

         •  Demand high performance and reliability over time (i.e., consistent
            and accurate  data  transmission without  need for recalibration);
            robustness (i.e.,  imperviousness to adverse  in  situ conditions);
            value  and affordability; adaptability; sustainability (both technical
            and institutional); real-time data transmission; and portability.

         •  Pursue a "systems"  approach for  sensor networks in  complex
            ecosystems.

         •  Deploy "early-warning" systems throughout the country that are
            relatively inexpensive, widespread networks that direct attention to
            deeper problems  as they develop.
Challenges
         •  Network  design  is perhaps more challenging than actual sensor
            development, and highly dependent on the specific objective and
            network scale.

         •  Wide  ranging potential  applications  were  discussed  including:
            mercury in air and water in the Eastern U.S.; Mississippi River
            watersheds and  Gulf of Mexico  dead zone;  the  Great Lakes;
            Chesapeake  Bay; TMDLs in Northwest redwood region;  and
            CAFOs in the San Joaquin Valley.
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 Future Role of the SAB
          •  A workshop on the use of new sensor network technology in the
             context of two or three Agency problems (e.g., Great Lakes). The
             developers as  well  as EPA problem identifiers are necessary to
             formulate templates and networks  in the context of the Agency
             strategic  and multi-year plans.   Such activities should be directed
             toward developing a clear conceptual model that delineates what
             the system looks like and how it works, to  appropriately  use
             sensors in hypothesis testing. Adaptive management would then
             allow continued use of the network.
9.0 INFORMATION TECHNOLOGY - LARGE SCALE COMPUTING

9.1    Invited Presentation:  Information Technology (IT): Implications for Future
       Science at EPA - Dr. Gregory McRae, Massachusetts Institute of Technology
       (See Slides Appendix J)

    Information   Technology  (IT)  includes   a  spectrum  of  computers, databases,
communications, sensors, visualizations, algorithms, and their management.  Information
technology can  also  reduce the time for applying science in environmental decision-
making.   Therefore, advances  in  information technology  have  the   potential  to
revolutionize how EPA might manage environmental decisions.  Dr. McRae focused his
presentation  on driving forces for change, new dimensions of working in teams, routine
visualization of complex phenomena,  and global environmental  problems.  Several key
points follow, and his complete slide presentation is shown in Appendix J.


          •   Real and perceived environmental risks exist, and IT  can help
             develop proper science and policy responses and revolutionize how
             EPA manages environmental risks.

          •   Computers,  databases,  communications,  sensors,  visualization,
             algorithms, and their management can reduce the time for applying
             science in environmental decision-making.

          •   Forces  driving  information  technology  development  include
             bandwith,   optical   networks,   remote   access,  and  routine
             visualization of complex environmental problems.

          •   IT can resolve complex environmental and energy  problems which
             often involve multiple and conflicting  objectives, asymmetric
             information, short decision cycles, long analysis  times, and few
             technically qualified people.
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•  Air Quality, Genomics, and Bioinformatics are areas where IT has
   been  used with  varying  degrees of success in  environmental
   problem solving.

•  Data for environmental problem solving are not often integrated,
   may lack useful uncertainty estimates, are variously documented,
   and  of variable  quality.   Solving these data problems  would
   enhance the pragmatic use  of IT  in  environmental decision-
   making.

•  Moving away from conventional compliance assessment to inverse
   modeling  and  deterministic  control  strategy  designs  would
   minimize  control costs  and  maximize  air  quality,  minimize
   exposure to pollution, and minimize risk of exceedances.

•  Solving these problems requires cost-effective monitoring systems
   using advanced technology.

•  The  development of novel inexpensive  sensors and innovative
   deployment  would  assist  the  detection  and   resolution  of
   environmental problems before they become acute.

•  Greater  use  of life  cycle  assessment  models  like  MIT's
   Environmental Evaluation Model would assist the development of
   optimized design and control strategies for new industrial products
   and processes.

•  Dr. McRae posed several needs and  questions for  the  SAB's
   consideration.

              -   There   is  a critical   need  for   multimedia
                 integration of databases and models to   prevent
                 problems such as MTBE contamination.

              -   A most critical issue is how to find and employ
                 people with appropriate training and expertise in
                 information technology

              -   Information  technology is  a  critical  enabling
                 resource and asked  if  EPA needs  a Chief
                 Technical Officer or Chief Information Officer?

              -   How can database access be improved for use in
                 decision- making?
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                        -   How can more science be integrated into control
                           strategy design processes?

9.2    Large Scale Computing Breakout Group Report
       The  breakout  group  participants  (Appendix  C)  discussed  possible
applications of information technology with an emphasis on supercomputing
applications, and prepared the following points for presentation and discussion at
the plenary session.

   Opportunities

          •  Information technology is crucial to advancement of computational
             chemistry,  computational   toxicology,  air  quality   modeling,
             biochemical  modeling, groundwater  transport  and remediation,
             watershed management, surface water quality and hydrodynamics.

          •  Additional opportunities include the use of information technology
             in  real time  emergency response; multimedia, ecological, and
             respiratory airway modeling

          •  Similarly,  information technology can be used to enhance the
             applications  in:    econometrics;  decision  analysis;  optimized
             economic  growth;  urban  system management;   and scenario
             analysis for  economic,  social and  environmentally  sustainable
             solutions under uncertainty

   Challenges

          •  Support and  educate a diverse  generation   of  scientists and
             engineers capable of using innovative and  state-of-the-art large
             scale computing applications.

          •  Data availability, access, and quality

          •  Model evaluation

          •  Computing capability

          •  Practical methods for large-scale optimization

          •  Prioritize resources to resolve uncertainties

          •  Collaboration between Agencies
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   Future Role of the SAB
             Create an  advisory panel to prioritize  opportunities  and
             identify challenges and outline necessary resources

             Organize a "supercomputing workshop"

             Put together a "supercomputing road map" of needs to be
             considered for EPA

             Inclusion   of  other  Agencies   with   supercomputing
             capabilities/interest

             Engage  industry  in   the  mission  so  they  can  share
             experiences and EPA can learn new technologies
10.0 CONVERGING TECHNOLOGIES

10 .1   Invited Presentation Summary:  Converging Technologies (NBIC) -Dr.
William Bainbridge, National Science Foundation (See Slides Appendix K)

       Converging technologies represents a movement focused on the unification of
science and  technology,  and is defined by  interactions  between  Nanotechnology,
Biotechnology, Information  Technology,  and Cognitive  Sciences (often referred  to as
NBIC  or convergence).  Dr. Bainbridge focused his presentation on general principles
and applications of convergence.   Several key points follow, and his complete  slide
presentation is shown in Appendix K.


   •   Opportunities for science and technology convergence are based on shared
       methodologies which provide opportunities  for developing transformative
       tools

   •   One-way convergence is taking an idea, tool or discovery from  one field
       and applying it to another.

   •   Mutual convergence  is when scientific  theories  and models are applied
       across different fields facilitating exchange.

   •   The principles of convergence include:

              Material  unity of nature at the nanoscale;

          -   Technology integration from the nanoscale;
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          Key transforming tools for NBIC;

          The concept of reality as a closely coupled complex hierarchical
          systems;

          Goals to improve human performance.

•  Application areas  for improving  human performance using converging
   technologies have emerged including:

          The expansion of human cognition and communication;

          Improving human health and physical capabilities;

       -   Enhancing group and societal outcomes;

           Strengthening national security and competiveness, and

           Unifying science and education

•  Several converging technologies application areas were presented.

          Spatial  cognition through wearable sensors and computers to
          enhance awareness of health, environment, potential hazards,
          natural resources, etc.

       -   National security  applications  including  information  rich
          fighter systems,  intelligence gathering systems, and effective
          counter measures  for biological, chemical,  radiological and
          nuclear attacks

          Agriculture and  food industry applications to increase yields
          through networks  of cheap,  smart  sensors  that constantly
          monitor the condition of plant, animal and farm products.

          New categories  of materials,  devices  and systems for use in
          manufacture, construction, transportation, medicine, emerging
          technologies and scientific research.

          Processes of the  living cell, which is the most complex known
          form of matter with nanoscale components.

       -   Principles   of  advanced   sensory,    computational   and
          communications  systems integrating diverse components into a
          ubiquitous, global network
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              Structure, function,  and occasional dysfunction of intelligent
              systems, most importantly the human mind.
10.2 Converging Technologies Breakout Group Report
    The breakout group participants (Appendix C) discussed actions the SAB might take
with respect to converging technologies.  The breakout group focused their attention on
priorities and prepared the following points for presentation and discussion at the plenary
session

    Highest priority SAB Actions

          •   New Reducing Risk/Over the Horizon-type  report focusing on
              where   existing regulatory science and policy lags behind new
              technology issues.

          •   Address Administrator's priorities by identifying opportunities for
              converging technologies (e.g., mercury).

          •   Review  EPA's  Science  Inventory  for activities  related  to
              converging technologies and identify gaps and opportunities.

          •   Develop  low-cost exploratory  steps  to  increase  fluency  in
              converging  technologies and  influence exchange within  and
              between EPA, other Agencies, and stakeholders.

          •   Develop joint proposals with other Federal Agencies

    Other Possible  SAB Actions to Highlight Opportunities and Address
    Challenges

          •   White paper on challenges and  opportunities— addressing national
              and  global dimensions

          •   Advise on EPA's plans to expand its skill set

          •   Catalyze multi-disciplinary collaboration - "Synthesis U,"
              rotational assignments, fellowships

          •   Data issues - meta data needs

          •   Address EPA's gap in cognitive and behavioral science

          •   Address environmental education, risk perception, risk
              communication issues
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  APPENDIX A




WORKSHOP AGENDA
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          Nanotechnology, Biotechnology, and Information Technology:
                    Implications for Future Science at EPA
                                   Agenda

Day 1 — Wednesday, December 1, 2004

              8:30 Welcoming Remarks -Dr. Granger Morgan, Chair, SAB
                   Workshop Introduction - Dr. Anthony Maciorowski, SAB Staff
                   Office

              8:45 Industrial Ecology Principles: A Unifying Theme For
                   Environmental Applications of New Technologies - Dr. Braden
                   Allenby, School of Engineering, Arizona State University

              9:15 Nanotechnology - Dr. Roland Clift, Centre for Environmental
                   Strategy, University of Surrey

              9:45 Biotechnology - Bioproduction - Dr. Harold G. Monbouquette,
                   Department of Chemical Engineering, University of California -
                   Los Angeles

             10:15 Biotechnology - Genomics - Dr.  Bruce Aronow, Cincinnati
                   Children's Hospital Medical Center

             10:45 Break

             11:00 Information Technology - Sensor Networks - Dr. Deborah
                   Estrin, Department of Computer Science, University of California
                   - Los Angeles

             11:30 Information Technology - Large Scale Computing/Modeling
                   Applications -Dr. Gregory McRae, Massachusetts Institute of
                   Technology

             12:00 Lunch

              1:30  Converging Technologies - Dr. William Bainbridge, National
                   Science Foundation

              2:00 General Discussion
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          Nanotechnology, Biotechnology, and Information Technology:
                    Implications for Future Science at EPA
                            Agenda (Continued)

Day 1 — Wednesday, December 1, 2004

       2:30  Breakout Groups
                   Nanotechnology
                   Biotechnology - Bioproduction
                   Biotechnology - Genomic
                   Information Technology- Sensor Network
                   Information Technology- Large Scale Computing/Modeling
                   Converging Technologies

             5:30  Adjourn for the Day

Day 2 ~ Thursday, December 2, 2004

              8:30  Breakout Groups (Continued)

                   Nanotechnology
                   Biotechnology - Bioproduction
                   Biotechnology - Genomics
                   Information Technology - Sensor Networks
                   Information Technology - Large Scale Computing/Modeling
                   Converging Technologies

             9:45  Breakout Group Reports

            10:45  Break

            11:00  Wrap up and discussion of next steps

12:30 Adjourn
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                APPENDIX B
BIOSKETCHES OF INVITED SPEAKERS AND SUBJECT
              MATTER EXPERTS
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      BIOSKETCHES OF INVITED SPEAKERS AND SUBJECT
                            MATTER EXPERTS

Industrial Ecology

Invited Keynote Speaker

      Professor Braden Allenby is  a Professor at Arizona State University's Ira A.
Fulton School of Engineering in the Department of Civil and Environmental Engineering.
Prior to that he was Vice President, Environment, Health and Safety at AT&T, an adjunct
professor at The University of Virginia's School  of Engineering  and at  Princeton
Theological  Seminary, and the inaugural Batten Fellow at Darden Graduate  School of
Business at the University of Virginia.  He is well known for his  work in industrial
ecology,  and works with information systems and technology from an earth systems
engineering  and  management  perspective, studying  the economic, environmental and
social implications of technological  systems,  communications,  infrastructure,  and
services.  Dr.  Allenby  as co-edited, authored and  coauthored numerous textbooks in
industrial ecology and systems engineering. He received his B.A. cum laude from Yale
University, J.D. and Masters in Economics from the University of Virginia, and Masters
and Ph.D. in Environmental Sciences from Rutgers.

Nanotechnology

Invited Speaker

      Professor Roland Clift is Distinguished Professor of Environmental Technology,
and founding Director of the  Centre  for Environmental Strategy at  the University of
Surrey.   He was previously  Head  of the  Department  of Chemical  and Process
Engineering  at the University  of  Surrey,  and is a visiting Professor in Environmental
System Analysis at Chalmers  University,  Goteborg, Sweden. He is a member of the:
Royal  Commission  on Environmental  Pollution;  International  Expert  Group  on
Application  of Life Cycle Assessment  to Waste Management;  and  the Rolls-Royce
Environmental Advisory Board. He is  a past member of the UK Eco-labeling Board, and
currently serves as an Expert Adviser to a House of Lords enquiry into energy efficiency.
In 2003, Professor Clift was awarded the Sir Frank Whittle Medal of the Royal Academy
of Engineering for outstanding and sustained engineering achievement contributing to the
well being of the nation.

Invited Experts

      Dr.  Catherine  Alexander has a  broad  background in communications that
combines study in social trends, public attitudes and communications methodology with
work  experience in the media, public  affairs  and scientific communications. Upon
graduation from  the University of Michigan (Ann  Arbor), Ms. Alexander  moved to
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Washington, DC where she became a television news producer and writer for the local
ABC affiliate.  After five years in that position, she worked in corporate communications
at a local energy company, serving as corporate spokesperson and news media manager.
Later, as an independent writer and producer, Ms. Alexander specialized in news  media
relations consulting and writing on alternative energy and environmental issues.  She has
worked for the  American  Association  for  the Advancement of Science  as Senior
Communications Officer  and as Vice President of Americans for  Medical Progress, a
group that advocates for biomedical research. Ms. Alexander became  Communications
Director for the National Nanotechnology Coordination Office, the secretariat of the U.S.
National Nanotechnology Initiative, in February 2003.    She has completed graduate
work at Johns Hopkins University and George Mason University.

       Dr.  Matteo Pasquali (Invited Subject Matter Expert) is an Assistant Professor of
Chemical and Biomolecular Engineering at Rice University, where he also serves  as co-
director of the Nanomaterials Production Facility of the NSF-Rice  Center for  Biological
and Environmental  Nanotechnology.  He is an active member  of the:   Center for
Nanoscale  Science and Technology; the Carbon Nanotechnology  Laboratory;  and the
Computer and Information  Technology Institute.  Before joining  Rice University,  he
earned a Ph.D. and  conducted post-doctoral work at the University of Minnesota.  Dr.
Pasquali's research interests  involve the interactions of flow and liquid micro- and nano-
structure in complex fluids, with application  to the processing of engineered materials,
with a particular focus on DNA solutions and carbon nanotube dispersions.

EPA Experts

       Dr.  Michael Gill is currently the ORD Hazardous  Waste Technical Liaison
(HSTL) for EPA Region 9.  This  position  is one of technical support and information
brokering. He  helps make the connection between hazardous waste technical  needs and
ORD  Lab  expertise. His customers  are for the most part  project managers in the
Superfund  Program, but may include  RCRA  and other  Regional  EPA staff, State
environmental staff, industry and the public. Mike also participates in research planning,
environmental technology demonstrations, and workshop planning. Mike has been in his
present position as HSTL since 1998 and has been at EPA since July of 1992, when he
was hired as a Remedial Project Manager in Region 9's Superfund Program.

       Ms. Marti Otto is  an environmental engineer in the Technology Assessment
Branch of  the Technology  Innovation  and Field Services Division of the  Office  of
Superfund Remediation and  Technology Innovation of the U.S. Environmental  Protection
Agency. Ms. Otto has almost 20 years of experience in  hazardous waste site  evaluation
and remediation  and environmental regulation  and policy development.  She earned a
Bachelor of Science degree in Biology and  a Master of Science degree in Environmental
Science and Engineering from Virginia Tech.
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       Dr. Dennis Utterback is a senior policy analyst in the Office of Science Policy in
EPA's  Office  of Research and  Development.    He represents  ORD  science policy
positions in  the  development of Agency decisions on toxic substances  and pesticides.
Specific  areas of expertise include flame retardants, PFOA, nanotechnology,  human
studies and cumulative risk assessment. He has  also designed and presented a half-day
course on risk assessment basics for a non-technical audience.   Prior to ORD, Dr.
Utterback led various workgroups in EPA's Office of Pesticide Programs, in managing
risks for  high risk chemicals and development of an  import tolerance policy.  He has a
B.A. in political science from Augustana  College and a Ph.D. in  public administration
from Syracuse University.
Biotechnology - Bioproduction

Invited Speaker

       Dr. Harold G. Monbouquette is a tenured professor in  the UCLA Chemical
Engineering Department.   Professor Monbouquette received an AB  in  Biochemical
Sciences  from  Harvard College and  a Ph.D. in Chemical Engineering from North
Carolina  State  University.  He conducts research on biosensors, the biotechnological
applications  of extremely  thermophilic  microorganisms,  and  on  the  molecular
engineering of  surfaces for materials and nanoelectronics applications.  He joined the
faculty at UCLA in 1987 as assistant professor.  He was a recipient of a Department of
Energy Young Faculty Award and was presented a TRW Excellence in Teaching Award.

Invited Experts

       Dr. Robert M. Kelly is a Professor in the Department of Chemical Engineering
at North  Carolina  State University.  He holds a Ph.D. in Chemical  Engineering from
North Carolina  State University. His research interests include biochemical engineering;
biocatalysis;  microorganisms from extreme  environments;  microbial  physiology  and
bioenergetics; functional genomics.  He has  served  as Director, NCSU NIH  Graduate
Student Biotechnology Training Program, and  as the Associate Vice-Chancellor for
Research Development, Research and Graduate Studies.

       Dr. Robert Mark  Worden is a Professor  in the Chemical Engineering  and
Materials  Science  Department of Michigan State University. He received a Ph.D. in
Chemical Engineering from the University of Tennessee. His research program integrates
recombinant-protein  production, biocatalysis,  and  nanotechnology  to  develop  new
systems  for  bioproduction, biosensing,  and bioremediation.  He  holds  patents on
microbiosensors for in situ use and cell-growth methods. He established and directs the
MSU Center  on Nanostructured Biomimetic Interfaces, a graduate training program on
Technologies for a Biobased Economy, the MSU Protein Expression Laboratory, and the
Multidisciplinary Bioprocessing Laboratory.
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EPA Experts

       Ms. April Richards is the Deputy Director of the Small Business Innovation
Research (SBIR) program for the U.S. Environmental Protection Agency (EPA).  The
SBIR program links new,  cutting-edge, high-risk innovations with EPA programs in
water and  air  pollution control, solid  and hazardous  waste management, pollution
prevention  and environmental monitoring. April is an environmental  engineer with
EPA's National Center for Environmental Research in the Office  of Research and
Development (ORD) where she works on several extramural research programs aimed at
developing environmentally friendly  technologies.   She  worked for 5 years in private
industry with an environmental  engineering consulting firm in Florida primarily in the
area of drinking water treatment.  She  has a  Master's  degree in Civil/Environmental
Engineering and is a licensed, professional engineer.

Biotechnolgy - Genomics

Invited Speaker

       Dr. Bruce Aronow is an Associate Professor of Pediatrics and Co-director of the
Computational Medicine Center  at the Cincinnati Children's Hospital Medical Center.
He received a Ph.D. degree from the University of Kentucky. His research group works
to identify similar and/or cis-element clusters in genes, and has developed a Web-based
tool called TraFac (Transcription Factor Comparison). TraFac identifies the cis-elements
in phylogenetic footprints. They are also working to identify compositionally similar cis-
regulatory element clusters in groups of co-regulated  genes, which may serve as valuable
probes for genome-wide identification of regulatory regions.  His group is also building
an integrated gene annotation tool with the capability  of user-added annotations.\

Invited Experts

       Dr. Mark Pershouse is an Assistant Professor in the Department of Biomedical
and Pharmaceutical  Sciences at the University  of  Montana.  He earned  a Ph.D.  in
Biomedical Sciences from the University of Texas at Houston, and was a Postdoctoral
Fellow at the M.D.  Andersen Cancer Center, and a  Postdoctoral Research Associate at
Baylor College of Medicine. His major research focuses on the genetic events, which
lead to human mesothelioma formation following  asbestos exposure. Through his role as
the University of Montana Microarray core director he  also focuses  on the molecular
response to toxicants such as asbestos, silicates, organophosphates, or metals, providing
new insight into response mechanisms of, and avenues for, therapeutic intervention.  The
characteristic cohort of genes responding to a stimulus can provides a toxic signature, and
offer tools for monitoring exposures and finding genes responsible for our individual risk
of disease following exposure.  He is  also collaborating through the core facility on such
diverse issues  as biomarkers of exposure to biological and chemical warfare agents, the
molecular and cellular response  to high altitude, and the  search for molecules which
direct innervation of skeletal muscle during development.
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       Dr. Parke Rublee is a Professor of Biological Sciences at the University of North
Carolina - Greensboro.  His interests are in aquatic microbial ecology  and a current
research focus is the development of microarrays for use in water quality assessments.
He has developed gene probes for the determination of the geographic distribution of
Pfiesteria  piscicida, a toxic dinoflagellate, which has been linked to fish  kills  in North
Carolina coastal waters.  He has  also addressed the structure and function of aquatic
microbial  food webs in Alaskan arctic lakes, and how genetic variability in Alaskan fish
populations relates to landscape features.

EPA Experts

       Dearfield,  Kerry L., b.  Washington, DC,  September 21,  1952, m.  '78, c.2;
Education: BS '74 (Biology) College of William & Mary, Williamsburg, VA; MS '78
(Cell  Biology) University of Pittsburgh, Pittsburgh, PA; Ph.D.  '84  (Pharmacology)
George  Washington  University  Medical   Center,  Washington,  DC.  Predoctoral
fellowship: '75-'78  graduate teaching fellowship. Current Position:  '03-present U.S.
Environmental Protection Agency, Senior  Scientist for Science Policy,  Office of the
Science Advisor, Washington, DC (help develop policies,  guidance, and directions to
address cross-cutting, high level EPA science priorities). Previous work experiences:
'95-'03  U.S.  Environmental Protection  Agency, Biologist/  Pharmacologist, Office of
Science Policy, Washington, DC;  other U.S. Environmental Protection Agency positions
starting in '84 include: '84-'87 Pharmacologist, Office of Toxic Substances and '87-95
Geneticist/Supervisory Pharmacologist, Office of Pesticide Programs; '79-'84 Research
Associate, Laboratory of Environmental  and Radiological Hazard Research, Department
of Radiology, George Washington University Medical Center, Washington, DC. Society
memberships: Environmental Mutagen  Society (EMS; Board of Councilors  '98-'01;
Executive  Board  '98-'00; Editorial Board;  Public Relations  and  Communications
Committee, '91-'00, Chair '97-'99; Program Committee, '91, '03), Genetic Toxicology
Association (GTA; Board of Directors  '88-'91, '98-'01; Chairman '89-'91), Society of
Toxicology (SOT), AAAS, Association  of Government Toxicologists (AGT; President-
elect  '04).   Scientific  interests:  development of genetic  toxicology  assays with
endogenous metabolic activation;  modes of action for  toxicity  (including mutational,
physiological and pharmacological mechanisms); use of genotoxicity data in regulatory
decisions   (heritable  risk, carcinogenicity,  general toxicity);   mutagenicity testing
guidelines; development  of science policy;  development and  use of peer review; risk
assessment  and  risk  management  issues.  Publications:   numerous   peer-reviewed
publications on: genetic toxicology of chemicals; genotoxicity in regulatory decisions and
guidelines; peer review  and  risk  assessment practices.  Address:  U.S.  Environmental
Protection Agency, Office of the Science Advisor  (8105R), 1200 Pennsylvania  Ave.,
NW, Washington, DC 20460.
       Dr. Robert Frederick is  currently a  Senior  Scientist  in  the  Environmental
Protection Agency's Office of Research and Development at the National  Center for
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Environmental Assessment (NCEA). With the Agency since  1984, his responsibilities
have included coordination of the Biotechnology Risk Assessment Research Program and
the risk  assessment  of genetically  modified  products.  He has served as an  EPA
representative  to the  National  Institutes of Health Recombinant  DNA  Advisory
Committee; a Federal Coordinating Biotechnology Research Subcommittee; the United
States-European  Community  Task Force on  Biotechnology Research; and as  EPA
coordinator of Office of Science and Technology Policy's crosscut on biotechnology
research. He is currently a member of the Evaluation and Advisory Board for the USAID
sponsored Program on Biosafety  Systems administered through the International Food
Policy Research  Institute.  Dr. Frederick has extensive international experience in the
development of biotechnology regulatory frameworks  and biosafety training programs.
From  10/93 to  9/96,  he was Executive  Secretary  of  the  Biotechnology  Advisory
Commission (BAG) at the Stockholm Environment Institute, Stockholm, Sweden. While
with BAG, he organized and taught in six international workshops on biosafety and
biodiversity in Nigeria, Argentina, Zimbabwe, Kenya, and  Sweden.  He has lectured and
instructed on biosafety  issues in  many  countries including Argentina, Chile,  China,
Cameroon, Colombia, Denmark,  Germany,  Hungary,  India,  Kenya, Malawi, Mexico,
Namibia, Serbia, South Africa, Sweden, Syria, Zambia,  and Zimbabwe.  In 2002, he
spent six weeks with the US Embassy in Lithuania evaluating the status  and potential for
biotechnology development in that country.  Dr.  Frederick has published more than 25
articles on biotechnology regulatory development and implementation and is a principal
author of a  training  manual for  Biosafety  and  Risk Assessment  in  Agricultural
Biotechnology available in English, French, Portuguese  and Spanish. He earned a PhD at
Michigan State University and did his postdoctoral work  at Tufts University School of
Medicine.
Information Technology - Sensor Networks

Invited Speaker

       Dr. Deborah Estrin is a Professor of Computer Science at UCLA and Director of
the Center for Embedded Networked Sensing (CENS). Estrin has been instrumental in
defining the research agenda for wireless sensor networks, first chairing a 1998 DARPA
study and then a 2001 National Research Council study. Estrin's research has focused on
the technical  challenges posed by these long-lived, autonomous, massively distributed
and physically coupled systems, with a particular focus on environmental monitoring. In
2002 she founded the NSF Science  and Technology Center for Embedded Networked
Sensing. During the earlier parts of her career Professor Estrin focused on the design of
network and routing protocols for very large, global networks. Estrin received her Ph.D.
in Computer Science from MIT (1985), her BS in EECS from UC Berkeley (1980), and
was  on the faculty of Computer Science at USC from 1986 through mid-2000. Estrin is a
Fellow of the ACM, IEEE, and AAAS and  serves on the NSF Advisory Committees for
CISE and ERE  Directorates, and on the National Research Council Computer Science
and Telecommunications Board (CSTB).
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Invited Experts

       Dr. David A. Caron is the Bayer Professor and Chair, Department of Biological
Sciences, at the University of Southern California.  He received the Ph.D. in Biological
Oceanography  from  the  Massachusetts Institute of Technology  and Woods Hole
Oceanographic Institute. His research interests focus on marine and freshwater microbial
ecology, with emphasis on trophic relationships among single celled microorganisms, and
the development of molecular biological approaches for studying the ecology of free-
living  microorganisms.   He has received the Mary Sears  Chair for Excellence in
Biological  Oceanography, WHOI Seymour Hutner  Prize (Society  of Protozoologists),
and is the President-Elect of the Society of Protozoologists.

       Dr. Yu-Chong Tai is a Professor of Electrical Engineering and Bioengineering at
the California Institute of Technology  (Caltech).  His main  research interest  has been
MEMS (including micro sensors  and actuators) since his graduate school  in  1983. He
graduated from UC Berkeley working on polysilicon micromechanisms and micromotors,
and he joined Caltech in 1989. At Caltech, he built the Caltech MEMS Lab, which is a
facility with 7,000 square feet of laboratory (including 3,000 square feet  of  class-100
clean room). His research interest is to  build integrated systems on a chip using MEMS
and nano technologies. Currently, he leads a group of about 20 researchers working on
various MEMS projects such as  integrated micropackaging, microfluidics, bio MEMS,
smart MEMS skins, lab-on-a-chip and micro power generator.  For the last few years, his
research  has  expanded significantly  into polymer MEMS,  especially  on  Parylene
material. Examples of his Parylene MEMS works include retinal implants and HPLC-on-
a-chip. He has more than 200 technical articles in the field of MEMS. He was involved in
many MEMS Conferences and, for example, he was the General Chairman of the 2002
IEEE MEMS Conference. He is also a  Subject Editor of the IEEE/ASME J. of MEMS.
He is a fellow of the Institute of Physics.

EPA Experts

       Dr. John A.  Glaser is a research leader for a team of scientists and engineers
investigating sustainable technology and biotechnology.  As research scientist with the
U.S. Environmental Protection Agency  in the Office of Research & Development at the
National  Risk  Management Research Laboratory  in Cincinnati,  Ohio he leads  the
NRMRL biotechnology research program that is investigating risk management  issues
related to the pesticide incorporated protectant crops. This research program involves the
investigation of remote sensing for monitoring the new crops, new computing capabilities
to model the development of resistance in pest populations, toxin assay standardization,
and  testing of existing  simulation  models  for  the evaluation  of  pest  resistance
development. He  was awarded an EPA Gold Medal for his  research on  the EXXON
Valdez oil spill in Prince William Sound, Alaska. As research leader in fungal technology
for treatment of soils and solids contaminated with hazardous waste, he received the joint
recognition of USD A and US EPA for the development of a field-scale technology using
lignin- degrading fungi. He led two research teams to develop unique bench-scale testing
facilities to evaluate  bioslurry  and compost treatment of hazardous waste contaminated
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soils to permit evaluation of the two technologies using contaminated field materials. He
has  organized a  NATO  advanced  research  workshop  on  "The  Utilization  of
Bioremediation to Reduce Soil  Contamination: Problems and Solutions" that was held in
Prague, Czech Republic. At this workshop fifty participants in attendance represented 26
nations. NATO has designed the workshop format to enhance the scientific and technical
exchange between the Eastern European republics  and the West with  the  desire of
improving understanding of our different cultures and societies. Dr. Glaser's current
work focuses  on  the  evaluation of technology and  products to meet the criteria of
sustainability,  e.g. transgenic crops and biobased production. Dr. Glaser has provided
technical evaluation of current treaty activities to ensure that they support environmental
laws and objectives. He has also been tapped to provide input to US EPA and USDA
contributions to the 2002 World Summit in Johannesburg South Africa.

Information Technology - Large Scale Computing/Modeling
Applications

Invited Speaker

      Dr. Gregory McRae is  the Professor of Chemical Engineering in the Department
of Chemical Engineering at the Massachusetts Institute of Technology. He received the
Ph.D. from  the California Institute of Technology.    His research interests include
atmospheric processes responsible for oxidant formation,  acid  deposition and global
climate change, particulate dynamics, and  chemical  transport  and transformations in
multimedia environments.  He  is also interested in molecular design and computational
chemistry,  applications of very  high  performance  computing, high-level  language
compilers and data visualization, and the design of cost-effective public policies for
environmental problems.   He  has received  numerous honors and awards for  computer
graphics  and visualization, and has served on various Government technical committees
(U.S. EPA, National Research Council, National Academy of Sciences, Sandia National
Laboratory, U.S. DOE).

Invited Experts

      Mr.  James   Kasdorf  is  the  Director  of  Special   Projects,  Pittsburgh
Supercomputing Center, Carnegie-Mellon University  and the University of Pittsburgh.
Mr.  Kasdorf  works  to influence the computing  industry and  technology futures,
especially processor and system architectures, system effectiveness and efficiency for
high-end applications  and large-scale storage servers.  Mr. Kasdorf was instrumental in
establishing the supercomputing centers at the University of Nevada - Las Vegas and the
University of Alaska-Fairbanks. His work with university-based supercomputing centers
gained international recognition  for Westinghouse in  1992  through  a  Computerworld
Smithsonian Award in the Science category.  In  1993, the Pittsburgh Supercomputing
Center won the Computerworld -Smithsonian Award in the Science category for its
simulations of protein  interaction with DNA.  He has  served on various panels regarding
high-performance  computing for DOE, NSF, NOAA and NIH.  He is currently on the
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steering committee of the IDC High Performance Computing User Forum, and serves as
president of the HP High Performance Computing User Group.
       Dr. Robert Romanosky is a Technology Manager for Power Systems Advanced
Research at  the U.S. Department of Energy,  National Energy  Technology Center in
Morgantown West Virginia.  He  received the  Ph.D. from West Virginia University in
analytical  chemistry/instrumentation.  His responsibilities include research activities in
Materials,   Coal  Utilization  Science,  Bioprocessing,  University   Research,   and
Computational Energy Sciences.  The Materials Program fosters exploratory research to
generate new  materials, ideas  and concepts to  improve the performance or cost of
existing fossil  systems or to enable development of new systems. The Coal Utilization
Program develops advanced sensors, controls,  and models for power generation.  The
modeling development effort works with the Computation  Energy Science Program to
speed development and reduces  costs of new power generation technologies.   The
Computational Energy Science work entails the development of science-based models of
fossil fuel  conversion phenomenon,  simulation  capabilities that couple unit processes in
advanced power generation technologies, and virtual power plant simulations.
       Dr. Christine A. Shoemaker is the Joseph P. Ripley Professor of Engineering,
School of Civil and Environmental Engineering, at Cornell University. She is also the
past Chairman of the Department of Environmental Engineering, and an elected Fellow
of the  American Geophysical Union.   She is the recipient of the Humboldt Research
Prize, as well  as the Julian Hinds Award of the American Society of Civil Engineers
(ASCE) for her leadership and research in ecosystems management,  water  resources
systems analysis, and groundwater modeling and protection.  She has encouraged women
in engineering for which she received the "Distinguished Educator Award"  from the
National Society of Women Engineers.  Prof.  Shoemaker Co-Chaired an international
project on Groundwater Contamination sponsored by SCOPE and the UNEP.  She has
participated in National Academy of Sciences panels on groundwater contamination and
pest management, on the Scientific Advisory Board of the ATSDR.
Converging Technologies

Invited Speaker

       Dr. William  Bainbridge  is  Deputy Director,  Division of  Information and
Intelligent Systems, of the Nationl Science Foundation.  He holds a Ph.D. in Sociology
from Harvard University. He has held NSF positions as the Director of the Knowledge
and Cognitive Systems  Program, the Human-Computer Interaction Program,  and the
Innovation and Organizational  Change  Program,  as well as academic  positions  in
Sociology and Anthropology (Towson  University,  Illinois State University,  Harvard
University, and University of Washington.  He  has  edited  and co-edited several books
including the Encyclopedia of Human-Computer Interaction,  Converging Technologies
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for Improving Human Performance, and Societal Implications of Nanoscience and and
Nanotechnology.  His research interests have focused  on the sociology  of religion,
family, Utopian communities, and  science  fiction.  He has received numerous NSF
awards for Collaborative Integration, Above and Beyond the Call of Duty, Collaborative
Excellence, and Program Management.

Invited Experts

       Dr. Robert St. Amant is an Associate Professor in the Department of Computer
Science at North Carolina State University.  He earned a Ph.D. from the University of
Massachusetts. His research interests include a blend of human-computer interaction and
artificial intelligence, with an emphasis on planning concepts. He is interested in building
intelligent tools to help users with complex  tasks.  Examples include interface softbots,
affordances and tool use,  cognitive modeling, and visualization assistance.

       Dr. Nathan Swami is the Associate Director of University of Virginia's Institute
for    Nanoscale    &    Quantum    Engineering    Science    &     Technology
(http://www.nanoquest.virginia.edu)  and a  Steering Committee member of Virginia's
statewide nanotechnology initiative.  His research interests are in the surface science of
nanoparticles for sensing applications,  and in  the  study of environmental  risks from
nanoparticles using scenario analysis within a regulatory structure. He received his Ph.D.
in Materials Science, at the University of Southern California in Los Angeles, conducting
research on novel fullerene and carbon nanotube materials. His prior work as a Principal
Scientist at Clinical Micro Sensors, Inc. (a Caltech  start-up) and Motorola Labs  was in
the area  of developing  microelectronic interfaces  to  molecular biology  for eventual
application  as DNA  sensors  and  lab-on-chip  devices.  He  served as Director of
Virginia’s statewide nanotechnology initiative (http://www.INanoVA.org/), from
2000-2002, and he joined  the  faculty at UVA’s  Electrical  &  Computer
Engineering      Department     in     2004.      For      more     information:
http://www.ee.virginia.edu/profile.php?ID=90.
EPA Experts

       Dr. Michael Brody is a senior environmental scientist with EPA's Office of the
Chief Financial Officer.  His major responsibilities involve building Agency capacity in
environmental futures analysis to support long-term strategic planning.  He recently led
an international  futures project at the North American Commission for Environmental
Cooperation. At the Organization for Economic Cooperation and Development he served
as EPA's senior technical lead to the development of the OECD Environmental Strategy
for the First Decade of the 21st  Century.  He also currently  manages an  environmental
capacity building assistance project with the Ministry  of Environment of Ukraine.  In
earlier work at  EPA,  he was a co-author of  EPA's Framework for Ecological  Risk
Assessment and  other reports on ecological risk assessment and management. He  also
managed projects in ecosystem  valuation and edited a special issue of the Journal of
Ecological Economics.  He  also led EPA training courses in environmental policy and
risk assessment, in  Eastern Europe.  He held previous positions with the US Fish and
                                      B41

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Wildlife  Service, and  with the National Oceanic  and Atmospheric  Administration
through a Sea Grant Fellowship, and earned his Ph.D. in Zoology from the University of
Texas at Austin.

       Dr.  Nora Savage obtained a bachelors degree in Chemical Engineering in May
1992 from  Prairie View A&M University, in Prairie View, Texas.  She received two
Masters degrees - one in Environmental Engineering and one in Environmental Science-
from the  University  of Wisconsin-Madison, in Madison,  Wisconsin in May 1995, and a
doctoral degree in Environmental Science from the same institution in August 2000.  She
has had summer internships at the Ernest Orlando Lawrence Berkeley  and Lawrence
Livermore  National Laboratories,  and  at  the Eastman  Kodak  Company as   an
undergraduate  student.    She worked for  seven years at the Wisconsin Department of
Natural Resources in the Air Monitoring Division in Madison while attending graduate
school.   In addition, she worked as a  mentor/counselor for  both high school  and
undergraduate  students through involvement in various  educational programs at UW-
Madison, including  serving as a Counselor for the Ronald E. McNair Program.  Upon
completion of her doctorate, she obtained a one-year post-doctoral research associate
position at  Howard  University, where she taught a senior-level Civil Engineering class
and worked on various educational initiatives at the graduate school.  She is currently
working  as an environmental engineer at the  Environmental  Protection  Agency  in
Washington, DC in  the Office of Research and Development. Her focus areas include
nanotechnology and environmental justice.  She is also involved in various civic and
scientific    organizations,   both   as    a   volunteer    and   as    a   member.
                                     B42

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         APPENDIX C




BREAK OUT GROUP PARTICIPANTS
            C-l

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NANOTECHNOLOGY BREAK OUT GROUP
Invited Speaker:

Invited Experts:


EPA Experts:



SAB Breakout Chair:

SAB Breakout Members:
Designated Federal Officer:
Dr. Roland Clift

Dr. Catherine Alexander
Dr. Matteo Pasquali

Dr. Michael Gill
Dr. Martha Otto
Dr. Dennis Utterback

Dr. Thomas Theis

Dr. Viney Aneja
Dr. Brian Dodd
Dr. Wayne Gray
Dr. Roger E. Kasperson
Dr. Reid Lifset
Dr. Randy Maddalena
Dr. Genevieve Matanoski
Dr. Armi stead (Ted) Russell
Dr. Gary Sayler
Dr. David Sedlak
Dr. Deborah Swackhamer
Dr. Lauren Zeise
Dr. Yousheng Zeng

Ms. Kathleen White
                                   C-2

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BIOTECHNOLOGY - BIOPRODUCTION BREAK OUT GROUP
Invited Speaker:

Invited Experts:


EPA Experts:

SAB Break Out Chair:
Dr. Harold Monboquette

Dr. Robert M. Kelly
Dr. Robert Mark Worden

Ms. April Richards

Dr. Michael McFarland
SAB Breakout Members:
Designated Federal Official:
Dr. Kenneth Dickson
Dr. Ivan J. Fernandez
Dr. Catherine Kling
Dr. Guy Lanza
Dr. Mark Miller
Dr. James Oris
Dr. John R. Smith
Dr. William Smith

Dr. Thomas Armitage
                                   C-3

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BIOTECHNOLOGY - GENOMICS BREAK OUT GROUP
Invited Speaker:

Invited Experts:


EPA Experts:


SAB Breakout Chair:

SAB Breakout Members:
Designated Federal Officer:
Dr. Bruce Aronow

Dr. Mark Pershouse
Dr. Parke Rublee

Dr. Kerry Dearfield
Dr. Robert Frederick,

Dr. James Bus

Dr. George Corcoran
Dr. Mary Davis
Mr. Keith Harrison
Dr. Katherine Kiel
Dr. James E. Klaunig
Dr. Michael Kleinman
Dr. George Lambert
Dr. Ulrike Luderer
Dr. Melanie Marty
Dr. Gina Solomon

Dr. Suhair Shallal
                                   C-4

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INFORMATION TECHNOLOGY- SENSOR NETWORKS BREAKOUT GROUP
Invited Speaker:

Invited Experts:


EPA Experts:

SAB Breakout Chair:

SAB Breakout Members:
Designated Federal Officer:
Dr. Deborah Estrin

Dr. David A. Caron
Dr. Yu-Chong Tai

Dr. John A. Glaser

Dr. Bob Twiss

Dr. Anna Alberini
Dr. Kenneth Cummins
Dr. T. Taylor Eighmy
Dr. William H. Glaze
Dr. StanleyB. Grant
Dr. Philip Hopke
Dr. Allan Legge
Dr. Joan B. Rose
Dr. Laura Steinberg

Mr. Fred Butterfield
                                  C-5

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INFORMATION TECHNOLOGY - LARGE SCALE COMPUTING BREAK OUT
GROUP
Invited Speaker:

Invited Experts:



SAB Breakout Chair:

SAB Breakout Members:
Designated Federal Officer:
Dr. Gregory McRae

Mr. James Kasdorf
Dr. Robert Romanosky
Dr. Christine A. Shoemaker

Dr. H. Barry Dellinger

Dr. Dallas Burtraw
Dr. John C. Crittenden
Dr. A. Myrick Freeman
Dr. William C. Griffith
Dr. Michael Kavanaugh
Dr. Charles Pittinger
Dr. Kathryn Saterson
Dr. Chris Walcek

Mr. Daniel Fort
                                  C-6

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CONVERGING TECHNOLOGIES - BREAK OUT GROUP
Invited Speaker:

Invited Experts:


EPA Experts:


SAB Breakout Chair:

SAB Breakout Members:
Designated Federal Officer:
Dr. William Bainbridge

Dr. Robert St. Amant
Dr. Nathan Swami

Dr. Michael Brody
Dr. Nora Savage.

Dr. Dave Rejeski

Dr. Gilles Bussod
Dr. Trudy Cameron
Dr. Bart Croes
Dr. Ted Gayer
Dr. Meryl Karol
Dr. Jill Lipoti
Dr. Morton Lippmann
Dr. M. Granger Morgan
Mr. Ralph Morris
Dr. William Pizer

Dr. Angela Nugent
                                   C-7

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             APPENDIX D






BREAK OUT GROUP DISCUSSION QUESTIONS
                D-l

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           BREAK OUT GROUP DISCUSSION QUESTIONS
Opportunities

1.      Which technologies within the scope of this break-out session are likely to offer
       the greatest application potential for protecting the environment?

2.      What opportunities may offer the greatest potential in the near term (3-5 years)?

3.      What science and research issues need to be addressed to take effective advantage
       of those opportunities?

Challenges

4.      What are likely to be the most significant challenges for environmental protection
       presented by these new technologies?

5.      Which challenges would be most urgent to  address in the near term (3-5 years)?

6.      What science and research and environmental policy issues need to be addressed
       to confront these challenges effectively?

Future Role of the SAB

7.      If the SAB is to prepare to help EPA meet those opportunities and challenges,
       how could it do so most successfully?
                                     D-2

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APPENDIX E - SLIDE PRESENTATION
     Industrial Ecology Principles
Nanotechnology, Biotechnology, and
 Information Technology Workshop
              Environment  for the 21st
                         Century
                         Brad Allenby
                   Arizona State University
       So long as we do not, through thinking,
       experience what is, we can never belong to
       what will be.
       The flight into tradition, out of a combination
       of humility and presumption, can bring about
       nothing in itself other than self deception and
       blindness in relation to the historical moment.
         irce: M. Heidegger, The Question Concerning Technology and Other Essays, translation by W. Lovitt (New
          Harper Torchbooks, 1977), "The Turning," p. 49; "The Age of the World Picture," p. 136.
  Dr. Braden Allenby
                           E-l

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APPENDIX E - SLIDE PRESENTATION
        Industrial Ecology Principles
                                 Nanotechnology, Biotechnology,  and
                                  Information Technology Workshop
                   Level        Method of Study      Main Impact                 Typical IE Design Issues
                                            (Physical v. Cultural)

              Artifact manufacture     Traditional         Physical       Energy consumption in manufacture; toxics in product
                              environment and
                              safety compliance
                               (end-of-pipe)

              Artifact over lifecycle     DfE, LCA         Physical       Understanding conditions of use; energy consumption in use;
                                                            end-of-life management
               Construction and
                maintenance of
                  networks

                  Services
               (e.g., broadband to
                  home)
              Social practices based
                 on services
               (e.g., teleworking)
              Knowledge economy/
                 infosphere
 Systems          Physical      Evolution of technology (from telephony to internet protocol,
engineering                     wireless); interactions of systems components; efficiency per
                            unit service; systems boundary

  N/A         Physical/Cultural    Definition of "service"; relationship of service to physical
                            network and social practices

  N/A           Cultural      Both short and long term impacts important (and may not
                            align); difficult to predict because of cultural component; triple
                            bottom line implications, especially social ("digital divide")
                            Impact on social constructs ("wilderness", "environment").
                            Enable postmodernist fragmenting of values?
                            Enable world as artifact (real time comprehensive monitoring
                            systems)?
                            Substitution of information for energy/materials?
                            End of "natural history" w/ human contingency built into
                            natural system?
              Future  Scan:  What  Do  We  Know
                    I. The future will be technologically discontinuous as rates of
                    technological evolution continue to accelerate - scenarios include:
                      - NBIC (nano, bio, ICT, and cogsci convergence - from "monkey arm" to
                        "brain in the plane")
                      - "Functional  immortality" within 50 years (Netbased or wetware, your
                        choice)
                      - Multicellular organisms from molecules, 10 to 30 years  (and scale ups -
                        grow tables, chairs) - Viruses already done.
                      - Increased biodiversity - but "built", not "natural"
                         "Custom reality" - integrate virtual and physical
                        "Custom reality" - cogsci and ICT introduce "animal tourism", where you
                        can map your cognitive system into that of another species
                        Large "natural" systems integrated into human systems, and therefore their
                        dynamics dominate - e.g., carbon cycle, hydrologic cycle, Everglades,
                        biosystems  at all scales become commoditized, and therefore "designed" by
                        humans.
   Dr. Braden Allenby
                                                                                  E-2

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APPENDIX E - SLIDE PRESENTATION

      Industrial Ecology Principles
Nanotechnology, Biotechnology, and

 Information Technology Workshop
          Future Scan: What Do We Know
              From the era of "simple systems" to the era of
              "complex adaptive systems"
               - Current policies, laws, and institutional structures assume
                 "simple" systems - that is, systems are knowable, exhibit
                 understandable causality, and are controllable
               - But emergent behavior of interest now arises from complex
                 systems: unknowable, uncontrollable, with causal links that
                 are indeterminate or at the least not clear
                 CAS knowable only in real time, requiring policy, design,
                 engineering, and management mental models shift from a
                 priori control and definition to realtime adjustment and
                 dialog
                 Examples of integrated human/natural CAS earth systems:
                 Everglades, Baltic and Aral Sea; urban systems; major
                 technology systems (e.g., transportation networks,
                 Internet); carbon and nitrogen cycles
          Future Scan: What Do We Know
              Ethical systems in age of CAS require serious
              augmentation
                - Traditional ethical judgments fundamentally based either on
                 intent or outcome as compared to norm
               - With CAS, cannot judge intent because agent cannot predict
                 system response; cannot judge based on outcome because
                 agent cannot know that a priori
                 Need to move to process-based  rather than outcome-based
                 ethics: has one chosen right process to interact with CAS
                 Engineering ethics micro-based (individual practitioner);
                 need macro-based (who or what is responsible for, e.g.,
                 Internet, biotech, or cogsci)
                 Ethical systems assume foundational truths; what happens
                 when the underlying cultural constructs become contingent
                 on rapid time cycle?
  Dr. Braden Allenby
                                   E-3

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APPENDIX E - SLIDE PRESENTATION

     Industrial Ecology Principles
Nanotechnology, Biotechnology, and

 Information Technology Workshop
         Future Scan: What Do We Know
             Fundamental structure of human cognition changing
             at accelerating pace
              - Internet becomes memory unit: facts stored in cyberspace,
                human brain increasingly provides questions and meaning
               - Technology as window through which humans perceive
                physical reality (Phoenix Zoo: kids raised on Nature Channel
                and Net time cycles can't identify with "realtime" animals)
              - "Reality" as built structure of particular cultural constructs
                ("jungle" vs. "rain forest"; "swamp" vs. "wetlands"; "natural
                - supernatural" vs. "natural - human" dichotomy;
                "wilderness - evil" vs. "wilderness - Edenic") - we used to
                be able to view constructs as fixed; now they are contingent
                How to think when all concepts are contingent and changing
         Future Scan: What Do We Know


           • What is "Human"?
              - Is there any "normal"?
              - By what right do we impose our idea of current
                biological and cognitive limits on future
                generations?
              - Who or what decides what is "human"?
              - Is that question already obsolete?
              - What part of "human" is contingent, and what
                part is  not - or is "human" all contingent?
              - Equity: who gets to evolve? Who decides?
                Ethics and politics: what kind of conflict will occur
                if- when? - elements of "human" deeply held by
                powerful religions to be absolute are
                demonstrated to be contingent?
  Dr. Braden Allenby
                                E-4

-------
APPENDIX E - SLIDE PRESENTATION

      Industrial Ecology Principles
                  Nanotechnology, Biotechnology, and

                   Information Technology Workshop
                 Bothersome Questions
               Should it be the Technology and Science Advisory Board, and shouldn't an
               Industry Advisory Board be added?
               Should EPA become a competency that diffuses itself throughout government?
               How will EPA, and government generally, develop the ability to dialog with,
               rather than regulate, complex human/natural systems?
               How will EPA develop the ability to operate on a time cycle that aligns with the
               phenomenon for which it is responsible?
               How will EPA function as its core conceptual foundations - "environment,"
               "wilderness," "nature" - become increasingly contingent, and change
               substantively over shorter time periods?
               What is EPA's role as the world increasingly becomes a product of human
               design?
               How does EPA avoid becoming more and more effective at less and less
               important tasks as environmental impacts are increasingly a function of strategic
               and non-environmental technological and business systems (can the nano model
               be generalized)?
                   Global Economic History:  1500 - 1992

                           World GDP     Per Capita     Per Capita
                            (indexed to    World GDP    (indexed to
              Date         1500=100)   (1990 dollars)   1500-100)
              1500


              1820


              1900


              1950


              1992
 100


 290


 823


2,238


11,664
 565


 651


1,263


2,138


5,145
100


117


224


378


942
   Dr. Braden Allenby
                                                       E-5

-------
APPENDIX E - SLIDE PRESENTATION
      Industrial Ecology Principles
                                   Nanotechnology, Biotechnology, and
                                    Information Technology Workshop
                    Energy Production and Consumption
                                   1800 -1990
             Production
                    Biomass
                       Coal
                        Oil
             Total Use21
             Total Use,
             Indexed to 1900
                    1800
                    1,000
                     10
                    400
                                 21
                           1900
                           1,900
                           1,000
                                    20
                           1,900
                                   100
                                       1990


                                       1,800


                                       5,000


                                       3,000


                                      30,000


                                       1,580
Year
1700

1800

1900

1950

1970

1990

2000
(est.)
              1700 - 2000

Withdrawals  Withdrawals
   (km )    (per capita)    Irrigation
              0.17
 110

 243

 580

1,360

2,590

4,130

5,190
                                   0.27

                                   0.36

                                   0.54

                                   0.70

                                   0.78
                                  0.87
                                     11
90

90

90

83

72

66

64
                                                         Industry   Municipal
                                       24

                                       25
   Dr. Braden Allenby
                                                                        E-6

-------
APPENDIX E - SLIDE PRESENTATION
       Industrial Ecology Principles
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                     Carbon Cycle  Governance System
                             Fossil Fuel Energy Production System
                               Fossil Fuel Power Plant
                               Fossil Fuel Power Plant
                               Fossil Fuel Power Plant
            Mobile Uses
           \fe.g., transportation^


                  Control Functions

               Input: B+ccMW
                  Fossil Fuel

               Output: CO. Emitted
                   CO2 Sequestered

               Target CO2 Concentration
               Metric: in Atmosphere
           Carbon  Cycle:  Earth Systems Engineering Schematic
                                    Biodiversity
                     Water cycle       and habitat      Nitrogen cycle
                                     systems
          Other cycles
                                                                  Engineering/
                                                                  Management
                                                                  of Earth
                                                                  system
                                                                  relationships
                          Earth
                          System
                        Engineering
                     Other       Energy      Ocean       Biomass
                    systems      system    fertilization    agriculture     Engineering/
                                                                  Management
                                                                  of carbon cycle
                               Fossil fuel  Fish farming,  Organic chemical
                              industry, etc.     etc       industry, etc.

                                                                  Traditional
                    Implementation at firm, facility, technology and process level   engineering
                                                                  disciplines
   Dr. Braden Allenby
                                          E-7

-------
APPENDIX E - SLIDE PRESENTATION
       Industrial Ecology Principles
Nanotechnology, Biotechnology, and
 Information Technology Workshop
            Earth Systems Engineering and Management Principles:
                                 Design and Engineering


             Earth systems engineering and management (ESEM) initiatives should all be characterized
          by explicit and transparent objectives or desired performance criteria, with quantitative metrics
          which permit continuous evaluation of system evolution (and signal when problematic system
          states may be increasingly likely).

          + Design, engineering, and implementation of ESEM initiatives must not be based on implicit
          or explicit models of centralized control in the traditional rigid sense. Rather than attempting to
          completely define or dominate a system, the ESEM professional will have to see themselves
          as an integral component of the system, coupled with its evolution and subject to many of its
          dynamics.  This will require a completely different psychology of engineering.

          •* ESEM projects should be incremental and reversible to the extent possible.

          •* ESEM should aim for resiliency, not just redundancy, in systems design. A resilient
          system resists degradation and, when it must, degrades gracefully even under unanticipated
          assaults; a redundant system may have a backup mechanism for a particular subsystem, but
          still may be subject to unpredicted catastrophic failures.

          + ESEM should aim for inherently safe, rather than engineered safe, design. An inherently
          safe system fails in a noncatastrophic way; an engineered safe system is designed to reduce
          the risk of a particular catastrophic failure mode,  but there is still a finite probability that such a
          failure may occur.
           Earih
              Earth systems engineering and management (ESEM) projects and programs by
          definition raise important scientific, technical, economic, political, ethical, theological
          and cultural issues.  The only appropriate governance model under these conditions
          is one which is democratic, transparent, and accountable.

          +  ESEM governance mechanisms should foster inclusive, multicultural dialog.

          +  ESEM governance models, which deal with complex, unpredictable systems,
          must  accept high levels of uncertainty as endogenous to the discourse, and view
          ESEM policy development and implementation as a dialog with the relevant
          systems, rather than a definitive endpoint.  ESEM governance systems should
          accordingly place a  premium on flexibility and the ability to evolve in response to
          changes in system state, and recognize the policymaker as part of an evolving
          ESEM system, rather than an agent outside the system guiding it.

          +  The ESEM environment and the complexity of the systems at issue require
          explicit mechanisms for assuring continual learning, including ways in which
          assimulation of the learning by stakeholders can be facilitated.

          +  There must be adequate resources available to support both the immediate
          ESEM project and the science and technology research and development
          necessary to ensure that the responses of the relevant systems are  understood.
   Dr. Braden Allenby

-------
APPENDIX E - SLIDE PRESENTATION
       Industrial Ecology Principles
Nanotechnology, Biotechnology, and
 Information Technology Workshop
             Earth Systems Engineering and Management Principles: Theory
             Only intervene when required and to the extent required (humility in the face of
          complexity).

          + At the level of earth systems engineering and management (ESEM), projects
          and programs are not just technical and scientific in nature, but unavoidably have
          powerful cultural, ethical, and religious dimensions.

          + Unnecessary conflict surrounding ESEM projects and programs can be
          reduced by separating social engineering from technical engineering dimensions.

          + ESEM requires a focus on systems  as systems, rather than as just constituent
          artifacts; a dynamic, rather than static, mental model of underlying phenomenon.

          + Boundaries around ESEM projects and programs should reflect real world
          couplings and linkages through time, rather than disciplinary or ideological
          simplicity.

          + Major shifts in technologies and technological systems should be evaluated
          before, rather than after, implementation.
   Dr. Braden Allenby
                                        E-9

-------
 APPENDIX F - SLIDE PRESENTATION

         Nanotechnology
Nanotechnology, Biotechnology, and

Information Technology Workshop
         UniS
             University of Surrey
                  NANOTECHNOLOGY


                     Professor Roland Clift,
                   Centre for Environmental Strategy,
                   University of Surrey, GUILDFORD,
                        Surrey GU2 7XH, UK
                              and
               Royal Society/Royal Academy of Engineering
                  Working Group on "Nanoscience and
                  nanotechnologies: opportunities and
                          uncertainties"
                                                Prof Roland Clift
         UniS
             University of Surrey

                  NANOTECHNOLOGY

            '  An emerging technology based on solid
              particles in the size range where their
              properties are determined by size and
              surface condition rather than bulk
              properties:

                 typically 1 - 100nm

                 (nm = nanometer = 10~9m)

                 comparable in size to viruses
                                                Prof Roland Clift
Dr. Roland Clift
                          F-l

-------
 APPENDIX F - SLIDE PRESENTATION
         Nanotechnology
Nanotechnology, Biotechnology, and
Information Technology Workshop
          UniS
              University of Surrey
               SOME POSSIBLE APPLICATIONS
             Evolutionary:
               - Batteries and fuel cells
               - "Smart" packaging and labelling
               - Electronics and displays
               - Catalysts and separation membranes
               - Paints and coatings
             Longer Term:
               - Lubricants
               - Composites
               - Components and prosthetics
               - Diagnosis and targeted drug delivery
             "Blue Sky":
               - Self-replicating robots and assemblers
                                                   Prof Roland Clift
          UniS
              University of Surrey

               THREE AREAS OF CONCERN

               1. Are the quantifiable benefits real? eg.
                 energy savings
               2. Health and environmental impacts of
                 substances and manufactured
                 products
               3. Social impacts of new and emerging
                 technologies
                                                   Prof Roland Clift
Dr. Roland Clift
                            F-2

-------
 APPENDIX F - SLIDE PRESENTATION
         Nanotechnology
Nanotechnology, Biotechnology, and
Information Technology Workshop
         UniS
             University of Surrey
                LIFE CYCLE ASSESSMENT
              Wastes and
              Emissions
                           PRIMARY RESOURCES
                                               Prof Roland Clift
         UniS
             University of Surrey

             HEALTH AND ENVIRONMENTAL
                           IMPACTS

              Hazard: human toxicity or ecotoxicity of
                      material

              Risk: includes probability that "receptor"
                   is actually exposed to material
                                               Prof Roland Clift
Dr. Roland Clift
                         F-3

-------
 APPENDIX F - SLIDE PRESENTATION
          Nanotechnology
Nanotechnology, Biotechnology, and
Information Technology Workshop
          UniS
              University of Surrey
               SOURCE     pathway     RECEPTOR
               Direct:
               - Inhalation
                   eg. workplace
                   Emissions from vehicles, combustion, etc
                   Natural sources; eg. volcanoes
               - Dermal exposure; eg. sun-screens; cosmetics
               - Ingestion; eg. In water

               Indirect: primarily via food chain
                                                     Prof Roland Clift
          UniS
              University of Surrey
                  BASIS FOR REGULATION
               • Toxicity estimation or testing (hazard):
                  - animal testing
                  - "in vitro" tests
                  - "in silico" tests (incl. QSAR's)
                  - epidemiology
               • Persistence
               • Bioaccumulation
                                                     Prof Roland Clift
Dr. Roland Clift
                            F-4

-------
 APPENDIX F - SLIDE PRESENTATION
        Nanotechnology
Nanotechnology, Biotechnology, and
Information Technology Workshop
         UniS
            University of Surrey
                 CAN NEW MATERIALS BE
           REGULATED AS "NEW CHEMICALS"?
             Probably, but there are unresolved
               questions:
             • size matters.... (surface area?)
             • surface properties matter....
             • How do these affect persistence and
               bioaccumulation?
             • Many products with small quantities
                                             Prof Roland Clift
         UniS
            University of Surrey
                 CAN NEW MATERIALS BE
           REGULATED AS "NEW CHEMICALS"?
            • How to define production thresholds?
            • Are current risk assessment procedures
              appropriate and sufficient?
            • Regulate as consumer products or as
              medicines?
            • Labelling of products?
                                             Prof Roland Clift
Dr. Roland Clift
                        F-5

-------
 APPENDIX F - SLIDE PRESENTATION
         Nanotechnology
Nanotechnology, Biotechnology, and
Information Technology Workshop
         UniS
             University of Surrey

           PRECAUTIONARY APPROACH IMPLIES

           • Presumption against release of nanoparticles into the
           environment:
                 Fuel additives (eg. cerium oxide)
                 Bioremediation (eg. iron; other metals)
                 End-of-life products

           • Nanoparticles likely to be made at point of use

           • Makes arguments for a moratorium on production
           irrelevant....
           • Unless and until nanoparticles become commodities, this is
           probably not a general issue anyway....

                                                Prof Roland Clift
         UniS
             University of Surrey
              Health and environmental
              impacts are uncertain.

              Social impacts are
              completely unknown...
                                                Prof Roland Clift
Dr. Roland Clift
                          F-6

-------
  APPENDIX G - SLIDE PRESENTATION
              Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Bioprocessing:  Opportunities & Challenges
                    Harold G. Monbouquette, Chemical Engineering Department,
                      University of California, Los Angeles, Box 951592, Los
                        Angeles, CA 90095-1592, harold@seas.ucla.edu
                  •  The broad universe of metabolic processes and
                     enzyme activities for exploitation
                  •  Specialty chemical bioprocessing: "Cheap stuff in,
                     expensive stuff out"
                  •  The promising biorefinery concept
                  •  New bioproducts and bioprocesses
Dr. Harold Monbouquette
                             G-l

-------
  APPENDIX G - SLIDE PRESENTATION
                Bioprocessing
                Nanotechnology, Biotechnology, and
                 Information Technology Workshop
                   Diverse Enzyme Toolkit of Industrial  Utility
                          Table 1.4. Classification of enzymes
                           Enzyme class     Number

                                   classified available
                                                    Reaction type
                                                                  Utility§
                          Oxidoreductases
90  Oxidation-reduction: oxygenation of C-H,

   C-C, C=C bonds, or overall removal or

   addition of hydrogen atom equivalents.
2.
Transferases
3.
Hydrolases
4.
Lyases
5.
Isomerases
6.
Ligases
720
636
255
120
80
90 Transfer of groups: aldehydic, ketonic, +
acyl, sugar, phosphoryl or methyl. -5%
125 Hydrolysis-formation of esters, amides, +++
lactones, lactams, epoxides, nitriles, 65%
anhydrides, glycosides.
35 Addition-elimination of small molecules ++
on C=C, C=N, OO bonds. -5%
6 Isomerizations such as racemization, ±
epimerization. -1%
5 Formation-cleavage of C-O, C-S, C-N, ±
C-C bonds with concomitant triphosphate -1%
cleavage
                          § The estimated 'utility' of an enzyme class for the transformation of non-natural substrates
                          ranges from +++ (very useful) to ± (little use) [83]. The values (%) indicate the percentage
                          of research performed with enzymes from a given class for the 1987-96 period.
                                         K. Faber, Biotransformations in Organic Chemistry, Springer, 1997
                     'Cheap stuff in,  Expensive stuff out": The
                                       Penicillin  Story

                      1939:  Penicillin culture concentration ~0.001 g/L

                      1940:  Unproven fermentation process chosen over
                      chemical synthesis

                      Microbiologists engage in mutation/selection;
                      Engineers designed large-scale submerged culture
                      process

                      1945:  Sufficient penicillin produced for ~100,000
                      patients

                      Current yields:  ~50 g/L; Chemical synthesis
                      approach still cannot compete!
Dr. Harold Monbouquette
                                                G-2

-------
 APPENDIX G - SLIDE PRESENTATION
          Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
              Can we engineer life for the
              economical, environmentally
              friendly production of
              chemicals?
                                    Glucose

                                     ate +
                                         HMG-CoA
                                   l-droxy-D-iylulose   i HMG-CoA reduoase
Dr. Harold Monbouquette
                     G-3

-------
  APPENDIX G - SLIDE PRESENTATION
               Bioprocessing
    Nanotechnology, Biotechnology, and
     Information Technology Workshop
                    Aromatic Biosynthesis  Pathways Lead  to
                                   Industrial Products
                     Pyrogallol
Chiral Drugs
Quinones

Adipic Acid
Vanillin
                        Chiral Drugs
                                    Chorismate
                                            Anthranilate
                                                       Advantages
     Aqueous solvent
     Moderate T, p
   • Non-toxic intermediates
     Lower raw material cost
                           Aspartame
                                            Frost & Drafts, Ann. Rev. Microbiol., 1995, 49, 557-579
                                      Primary    Seeondajy
                                     uilermedian:   Process
                                      Products
Dr. Harold Monbouquette
                                    G-4

-------
  APPENDIX G - SLIDE PRESENTATION
              Bioprocessing
           Nanotechnology, Biotechnology, and
            Information Technology Workshop
                    Global Material  & Energy Balances for
                    Assessment of Environmental  Impact
                            PLA1
                          Current peac
                   la' enefc&'_
                     iatartate
                  Carbon dtowto
                                  OKOfTI'V.lOltS
                                            1 Ha<
                                                       L

                                                         Sugars
                                                        Bore finery |  an
Cwbon dio
& water

Sieam
K)# ^


i
*T

4

Ljclidc
I
PLA
— 1 	
1

1


                           Polylactlcto
                                                       PolylKtkta
                                     E.T.H. Vink, K.R. Rabago, D.A. Glassner, B. Springs, R.P. O'Connor,
                                     J. Kolstad, P.R. Gruber, Macromol. Biosci. (2004) 4, 551-564
                   Slow Enzymatic Conversion  of Cellulosics
                     Avoided by Bioprocessing of Biomass-
                               Derived Synthesis-Gas
                                         Vent
      	 Liquid
      	 Gas
      	Foam
                         Nutrient'
'. pH Control i
                                                      Mf.Sjnthesis
                                                          Gas
                                                  Microbubble
                                                   Generator
                      Figure 5. Schematic diagram of a microbubble-sparged, syn-
                      thesis-gas fermentation with a membrane-based cell-recycle
                      system.
                              M.D. Bredwell, P. Srivastava, R.M. Worden, Biotechnol. Prog. (1999) 15, 834-844
Dr. Harold Monbouquette
                                         G-5

-------
 APPENDIX G - SLIDE PRESENTATION
          Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
             Can enzymes be integrated into
             chemical synthesis processes
             to improve economics and to
             reduce environmental impact?
Dr. Harold Monbouquette
                    G-6

-------
  APPENDIX G - SLIDE PRESENTATION
               Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                     The A  fulgidus Ala nine Dehydrogenase
                   Alanine dehydrogenase catalyzes the reversible conversion of
                   pyruvate to L-alanine:
                                NH + + NADH + H+<->
    + NAD+
                   Specific activity in the aminating direction: 203 U/mg
                   (Unit defined as  1 umol NADH oxidized per minute at 86 °C)

                   Biocatalytic applications include the synthesis of L-amino acids such
                   as: L-alanine, 3-fluoroalanine, L-serine, and 15N-labelled L-alanine
                           Temperature Dependence
                                         203 U/mg
                              40    60    80   100
                              Temperature °C
Dr. Harold Monbouquette
                               G-7

-------
  APPENDIX G - SLIDE PRESENTATION
                Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                                   L-Alanine Reactor
                      Demonstrate AlaDH from A. fulgidus is <
                      effective biocatalyst at room temperatun

                      2nd enzyme (yeast formate dehydrogen
                      for NADH regeneration
     al Conditions
     iM pyruvate
     mM NH3formate
     mM Tricene pH 8.0
    mMNAD
     U/ml FDH
     5U/mlAlaDH
                      Alanine dehydrogenase purified 3 mont.._      	 	
                      before reactor set-up and stored in solution at   Room Temperature
                      room temperature
                        NH3+pyruvate
                           L-alanine
Dr. Harold Monbouquette
                                 G-8

-------
  APPENDIX G - SLIDE PRESENTATION
               Bioprocessing
        Nanotechnology, Biotechnology, and
         Information Technology Workshop
                      Bioprocesses Provide New Products
                        Chiral drugs, flavorings, aromas,
                        herbicides, pesticides

                        Polylactic acid

                        Hyperthermophilic glycoside hydrolases for
                        oil/gas well fracturing
                       * Estrogen  Active site
                Estradiol   Receptor
                  Enzyme Inhibited
Enzyme Active
                                                                  Electrode

                                                       Detection Methods
                • APGP: p-aminophenyl p-D-galactopyranoside     Optical
                 ONPG: o-nitrophenyl p-D-galactopyranoside  Color formation
                Electrochemical
                  Current flow
Dr. Harold Monbouquette
                                       G-9

-------
  APPENDIX G - SLIDE PRESENTATION
             Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
g
^ 80
B
0 60

n
E
N1 40
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LU
0
~ 20
"o
OL
V~,J--~~
I /
i /
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_


-
-••-Bisphenol-A
-••-Genistein
-*--Nonylphenol
i i i i i i i i
0 1000 2000 3000
4000 5000 6000 7000 80
EDC Concentration (ppb)
                             Opportunities
                   Genomics enables design of life for environmentally
                   friendly synthesis of chemicals from renewable
                   resources
                   Enzymes improve selectivity and yield of chemical
                   synthesis processes at moderate conditions thereby
                   reducing environmental impact
Dr. Harold Monbouquette
                          G-10

-------
  APPENDIX G - SLIDE PRESENTATION
              Bioprocessing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                               Challenges
                    What is the true environmental impact of a biorefinery?

                    Plant/microbe/bioprocess design for better cellulosics
                    utilization

                    Limited knowledge of metabolism, including control
                    mechanisms

                    Enzyme identification/design/evolution for synthetic
                    reactions

                    Health/environmental effects of new classes of
                    products
Dr. Harold Monbouquette
                           G-ll

-------
APPENDIX H-SLIDE PRESENTATION
        Biotechnology-Genomics
                         Nanotechnology, Biotechnology, and
                          Information Technology Workshop
            Towards Genomics-based Analyses
             of Environmental Agent-Impact on
                        Biological Systems
                          Bruce Aronow, Ph.D.

                          Associate Professor and Scientific Director
                          Center for Computational Medicine
                          Cincinnati Children's Hospital Medical Center
                          College of Medicine, University of Cincinnati
                         MMHCC
                         the Mouse Models
                         of Human Cancers Consortium
           Environmental Agents Can Act at any Level of Genomic Information Flow

                     Structure ^^^^^  Activity i      ==£> Function
                Tissue Dynamics   Systems Biology    Outcome: Clinical, Population
            in
            o
            E
            o
            c
            
-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
                                      Nanotechnology, Biotechnology, and
                                       Information Technology Workshop
                     Environmental Agent Impact on
                              Biological Systems
              Environmental
               Agent Impact
                     Biosphere
                      • population loss
                      • environment niche disruption
                      • species sensitivity
                      • loss of biodiversity

                     Individual Organism
                      • morbidity
                      • mortality
                      • risk

General Goals for Impact Assessment

   Monitoring:
      • environmental damage
      • environmental agent-induced morbidity and mortality

   Prediction:
      • dangerous environmental agents
      • at risk populations, species
      • at risk environments
      • sensitive individuals
                                                                 QuickTime™ and a
                                                              F (Uncompressed) decompressc
                                                              are needed to see this picture.
             Using Genomics to Aid in the Assessment of Environmenta
             	Agent-Impact on Biological Systems	
             Environments
                      Organisms
                      Environmentally
                      Damaging Agents
Biological    Genomics-based
Impacts
                                                                Monitoring of
                                                               Biological Impact
                                                      Biological Impact Prediction
Dr. Bruce Aronow
                                                                                   H-2

-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
                      Nanotechnology, Biotechnology, and
                       Information Technology Workshop
                           >gy Approaches for the Integration of Genomics Data
                and Analysis into Assessment of Environmental Agent-Impact
             Model Systems:         Construction of
             • Knowledge-Generation     GenomiC/ProteomiC
             • Reference Database Creation Expression Databases
                                     Toxic Agents/Altered
                                     Environments Impacting
                                     Organisms/Systems
             Real-World Monitoring:
             • Impacts
             • Etiologies
                             damaging agent exposures
                                                               non-toxic
                Tissues
            Organisms
      Environmental
      Agents
                                                      predictor
                                                     biomarker _
                                                        genes m
                                                         '  I
                                                           \
              Creation of Systems Biology Integrated Databases:
                 Multi-Organismal, Tissue, Toxic Agent, Clinical, Phenotypic,
                 Genetic, Gene Expression Databases	
             Whole
             Genome
             Analyses
                                                    Analysis Goals
                                                        v  • Affected Samples
                                             •Identify     1  -Activated
                                             •Characterized  Pathways
                                             •Predict     f  • Cryptic Agents
                                                        )  • Therapeutic
                                                            Opportunities
                                                           • Preventable/
                                                            Critical Situations
                Infectious Agent
           Sensitive Individuals
        Toxic Agents
   Disease/Tumor
Normal tissue/Cell-type profiles
Dr. Bruce Aronow
                                                                                    H-3

-------
APPENDIX H-SLIDE PRESENTATION
         Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop
              Genomics-based  Monitoring of Environmenta
              Agent Impact: Obstacles
             Technical Barriers
                • Quantitative methods
                • Lack of:
                   • full genomic sequences
                   • references and technical standards
                   • annotation datasets of a range of "at-risk" populations
                • Heterogeneity of descriptor data

             Immaturity of Scientific Knowledge
                • Lack of knowledge of:
                   • cross-species and -environment generalization
                   • genes that confer environmental agent sensitivity
                • Poor assessment of normal states and damage indices for environments
                • Lack of reference data for model environmental agent-induced morbidity
                 and mortality
            Optimizing Distributed, Synergistic Use of MicroArray Technology:
                  experimental design considerations for evaluation
                  of platform performance and data reliability
"A-E1

dilution experiment


Day 1 whole
mouse RNA (%)
Mouse adult colon
RNA (%)

A
100
0


75
25


50
50

D
25
75
E
0
100
Samples: 990 D 0
                  Only 3 patterns
              should be observable
                               Rel.
                              exprs
Dr. Bruce Aronow
                                                                                H-4

-------
APPENDIX H-SLIDE PRESENTATION
        Biotechnology-Genomics
 Nanotechnology, Biotechnology, and
  Information Technology Workshop
           Evaluting the use of a "Universal Reference" calibration
             standard in experimental design: data normalization
                        Before...
        after
                A B C D Ej A BCD E^A B C D E /. B C D E
                 CHMC  NCI   StJudes Vanderbilt
A B C D E^ A BCD E^A B C D E^ /, B C D E
 CHMC   NCI   StJudes Vanderbilt
           Cross-Institutional  Reproducibility
Dr. Bruce Aronow
                                                                      H-5

-------
APPENDIX H-SLIDE PRESENTATION
        Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop
               Cross-Platform Reproducibility

              Slope from linear least square regression approach to
                      the comparison of reproducibility
              Construction and Mining of a Generalized Mouse Gene
                            Expression Database
            8734 genes X 100 samples X 2 replicate arrays
Dr. Bruce Aronow

-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Tissue and Organ-Specific Gene Expression
                      nervous system tissues
                                                       GI tract samples
                                            liver samples
                   Mouse Genes Highly Expressed in CMS
                                               Data description:
                                               a Novartis U74A dataset
                                               a 13 CMS tissues (2 replicates)
                                               a 32 non-CNS tissues and cell lines


                                               Filters:
                                               a 1.3 fold change in average
                                                 expression
                                               a Welch t-test, p = 0.05, Benjamin!
                                                 and Hochberg false discovery
                                                 rate
                                               a 674 probesets were identified;
                                                 564 unique genes with symbol
Dr. Bruce Aronow
                                                                                 H-7

-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                      Human Genes Highly Expressed in CNS
                                                Data description:
                                                a Novartis U133Adataset
                                                a 22 CMS tissues (2 replicates)
                                                a 57 non-CNS tissues and cell lines


                                                Filters:
                                                a 1.5 fold change in average
                                                 expression
                                                a Welch t-test, p = 0.05, Benjamin!
                                                 and Hochberg false discovery
                                                 rate
                                                a 1350 probesets were identified
/ Human \ / Mouse \
CNS Ixi 	 r^ CNS
\ Genes /'^ 	 ^' \ Genes /
Check Expression Pattern of
Ortholog Gene Pairs Present in
Both Genomes

Human CNS Human CNS
(strong) (strong)
Mouse CNS Mouse CNS (weak)
(strong) 1 148 genes |
302 genes
Human CNS (yes) Human C
Mouse CNS (no) Mouse Cf
114 genes 56 ge
I I 596 Genes
CZ 1 766 Genes

Human CNS (weak)
Mouse CNS (strong)
146 genes

NS (no)
MS (yes)
nes

Dr. Bruce Aronow

-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
   Nanotechnology, Biotechnology, and
    Information Technology Workshop
                 Expression Pattern Similarity of Genes Highly
                     Expressed in CMS of Humans and Mice
                                  Human non-CNS
                                  Mouse non-CNS
                                                                  478 Human-
                                                                  Mouse
                                                                  ortholog pairs
                                                                  from 596
                                                                  previously
                                                                  selected
                                                                  genes
Human Mouse
 CMS   CMS
                Can Normal Colon Development Provide Insight
                Into Colon Cancer Gene Expression Programs?

                        E13.5   E14.5    E15.5     E16.5      E17.5      E18.5
                          Jl     Jl      JJ        Ji         Ji         Ji
                   Pooled twenty individual embryonic colon RNA samples from each time point using CD-1
                           outbred strain, and then repeated using C57BL/6 inbred strain

                          U     Ji      Ji        Ji         Jl         Ji
                     Pooled samples from each time point underwent two rounds of linear amplification
                          U     Jl      Jl        Ji         Jl         Jl
                    Cy5-labeled cDNA from linearly amplified product from each time point and from both
                      strains hybridized with Cy3-labeled C57BL/6 E17.5 total pup cDNA as reference
                              Hybridizations repeated 3X each, dyes not switched
                   Cy5-labeled amplified
                   embryonic colon cDNA
        Cy3-labeled C57BL76 E17.5
         total pup cDNA reference
                     15K NIA staged mouse embryonic cDNAs and 5K Research Genetics mouse cDNAs
Dr. Bruce Aronow
                                                                                       H-9

-------
APPENDIX H-SLIDE PRESENTATION
         Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop

Comparative Profiling of Mouse Models of Human
Colon Cancer: Chemical & Genetic Tumor Models
Model
AOM_
chemical
ApcMin_BR-
SW_F1
ApcMin
_C57BL6
Smad3 -/-

Tgfbl-/-
Lab
Threadgill
Dove
Groden
Graf
Coffey
Doetschman

25
47*
47*
15
6
9
RNA
Isolate
d
13/25
10/34
10/34
10/15
6/6
3/9
Intact
RNAf
6/10
7/10
7/10
5/10
5/6
3/3
RNA
Amplified
6/6
5/7
5/7
5/5
5/5
3/3
MicroarrayJ
6/6
4/5
4/5
5/5
5/5
3/3
t Intact RNA is defined by Bioanalyzer and photometry
J Vanderbilt 20k chips with amplified RNA

Dr. Bruce Aronow
                                                                             H-10

-------
APPENDIX H-SLIDE PRESENTATION
         Biotechnology-Genomics
                          Nanotechnology, Biotechnology, and
                           Information Technology Workshop
               Comparative Transcriptional Profiling for Independent
                      Genetic Mouse Models of Colon Cancer
                                                              t
                                                        Pool of 4991
                                                        genes
                                                        Identified by
                                                        ANOVA X
                                                        Fold-Rank for
                                                        Model-
                                                        Specific
                                                        Colon Tumor
                                                        Expression
                  i i i i i i i i i i Ji i i 1 5 1 i i s i i I ; f * J ' * > ! < ' » • I i i i 1 1 i i i i i i i 5 is i
                               iIII4JiJ«aA4
AOM
                            ,..riIII'-"-i---'D-.«i"i
                              MIN           TGFbl  SMAD
                Discovering a Gene Expression Signature for
                 Chemical Exposure-Induced Colon Cancer
                                         ,420 AOMclusta
                                                         420 genes
                                                         with AOM-
                                                        Specific Colon
                                                           Tumor
                                                         Expression
                                                        (BH-FDRp<0.001)

                                                           AOM tumor model:
                                                         David Threadgill, PhD
                                                            UNC Chapel Hill
                                                        NIEHS Toxicogenomics
                                                         Research Consortium
                   developing colon   AOM    TGFb1  SMAD
                                  -MIN
Dr. Bruce Aronow
                                                                          H-ll

-------
APPENDIX H-SLIDE PRESENTATION
          Biotechnology-Genomics
    Nanotechnology, Biotechnology, and
     Information Technology Workshop
                  Microarray Analysis of 288 Independent Human Colon
               Cancers: global approach to tumor sample subclassification
                          and gene expression pattern discovery
             HG-U133 plus 2.0
                    Filter: Genes  ,
                    Overexpressec '"
                    in>12of288  1
                    human colon  *i
                    tumor samples *
               20,287 PS
                         hierarchical
                         clustering
                         of genes
                         and tumors
             Sergio Kaiser-Cincinnati
             Walter Jessen -Cincinnati
             Tim Yeatman-Moffit Cancer Center
                 Classification of Human Tumors as "Mature" or "Immature" by the
                   Behavior of Developmentally Regulated Mouse Gene Orthologs
                           "subtypes"   A BCEFG
                          1455 mouse
                          immature         |
                          genes
                                             J K L M N
                                                                     probesets
                   Top-1000 tumor
                   correlated human
                   gene orthologs of
                  mouse genes up and
                   down-regulated
                  during normal mouse
                  colon development
                                                                    probesets
                                    "immature" (I)
                                    tumors
"mature" (M)
tumors
Dr. Bruce Aronow
                                                                                      H-12

-------
APPENDIX H-SLIDE PRESENTATION
         Biotechnology-Genomics
Nanotechnology, Biotechnology, and
 Information Technology Workshop
              Expression profile-based Human Tumor Subtypes Show
                     Strong Differences in Survival Outcomes
                                              116 primary colon Ca tumors
                                              with > 36 Mo F/U

                                              Strong gene expression
                                              pattern-based discrimination
                                              of differential survival groups
                                              Tim Yeatman group
                                              Moffitt Cancer Center
             The Human Interactome Powers Systems Biology
                 Approaches to the  Dissection of Disease
/Johannes Freudenberg
/Ashima Gupta
/Anil Jegga

Siva Gowrisankar / Pediatric
Jing Chen /B\omed\ca\
Sue Kong /Informatics
Sarah Williams NIEHS Comparative
Mouse Genome
Consortium


NCI Mouse Mode/I^
of Human Gamier
Consortium

NCIC
Cl
Theresa Setty Center for Digestive D
Steve Connolly computational Ruesearch
Sergio Kaiser Medlcme

Walter Jessen
Jeremy Aronow Cathy Ebert
... . * Jennifer Marler
Vivek Ramaswamy
John Kleimeyer
Michael Kleimeyer


irectors
lallenge
sease
Serfter

Amy Moseley
Jianhua Zhang
David Witte
Sue Thompson
Bob Coffey
Young/Park
Shay/n Levy
David T
[
Rich
Joanna
TimR
Da
Tom Doe
An
Gr«
Tim

Andrew
Jordan Stc


readgill
till Dove
Halberg
Groden
eichling
i Carson
tschman
ly Lowy
g Boivin
Yeatman

Conway,
ckton \f

/
Dr. Bruce Aronow
                                                                        H-13

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
                    Nanotechnology, Biotechnology, and
                     Information Technology Workshop
              C=NS
                   Wireless Sensor Networks for Environmental Monitoring
                                          Deborah Estrm
                                     (Dave Caron, Tom Harmon)
                      Work summarized here is largely that of students, staff, and other faculty at CENS
                    We gratefully acknowledge the support of our sponsors, including the National Science
                   Foundation, Intel Corporation, Sun Inc., Crossbow Technologies Inc., and the participating
                                             campuses.
             ENTER FOR EMBEDDED NETWORKED SENSIN
                                                        •UCLA USC UCR CALTECH CSU
                                   Embedded Networked Sensing
            Ecosystems, Biocomplexity
            Marine Microorganisms
Micro-sensors, on-
board processing,
wireless interfaces
feasible at very small
scale-can monitor
phenomena "up close"

Enables spatially and
temporally dense
environmental
monitoring

Embedded
Networked Sensing
will reveal previously
unobservable
phenomena
                                                              groundwate
    Contaminant Transport
Seismic Structure Response
            CENTER FOR EMBEDDED NETWORKED SENSIN
                                                  •UCLA USC UCR CALTECH CSU  UC MERGED
         Dr. Deborah Estrin
                                                       1-1

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                                       Remote and In Situ Sensing
               • Remote sensing has transformed observations of large scale phenomena

               • In situ sensing will similarly transform observations of spatially variable
               processes in heterogeneous and obstructed environments
               SPOT Vegetation             Predicting Soil Erosion Potential:   Sheely Farm 2002
               Daily Global Coverage         Weekly MODIS Data             Crop map
               SWIR 3 Day Composite

                San Joaquin River Basin
                Courtesy of Susan Ustin-Center for Spatial Technologies and Remote Sensing
                           Environmental Monitoring Applications Exhibit
                             High Spatial Variations and Heterogeneity
                   Overflow of embankment
                                                     Precision Agriculture, Water quality
                                                     management
                  Algal growth as a result of eutrophication
                  -image courtesy of The J. for Surface Water Quality Professionals
                                                    •UCLA USC UCR CALTECH  CSU  UC MERGED
            CENTER FOR EMBEDDED NETWORKED SENSIN
          Dr. Deborah Estrin
                                     1-2

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
                          Nanotechnology, Biotechnology, and
                           Information Technology Workshop
                             Environmental Application Drivers at  CENS
               Contaminant Transport, Soils
               (Harmon, Allen)
                - Three dimensional soil monitoring
                - Error resiliency at node and
                  system level
                - Data assimilation, model
                  development

               Marine microorganisms
               (Caron, Requicha, Sukhatme)
                - Aquatic operation
                - Micro-organism identification
                - Sensor driven  biological sample
                  collection
               Biology/Ecosystem
               (Hamilton, Rundel)
Processes
                -  Robust, extensible microclimate
                   monitoring
                -  Image and acoustic sensing
                -  Infrastructure based mobility
                                                    UCLA USC UCR CALTECH CSU UC MERCEC
            CENTER FOR EMBEDDED NETWORKED SENSIN
             C ^NS      Wastewater reuse in the Mojave Desert
               •  Where does the County
                  Sanitation District (CSD) of Los
                  Angeles put 4 million gallons per
                  day of treated wastewater in a
                  landlocked region?

               •  Stakeholders:
                   - County Sanitation District
                   - Farmer
                   - Water Quality Board
                                                      sReclaimed wastewater
                                                        irrigation pivot plots
                  Palmdale, CA
                  wastewater treatment  *
                  plant
            CENTER FOR EMBEDDED NETWORKED SENSING
                                                   •UCLA USC UCR CALTECH CSU UC MERCED
          Dr. Deborah Estrin
                                                              1-3

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                                  Locally dense surface and subsurface
                                              sensor networks
                Modular "clustered" sensing targeting specific questions
                 -  What is quantitative flux of nitrate past the plant's
                    root zone?
                 -  What are the spatiotemporal variations associated
                    with nitrogen biogeochemical cycling in the soil?
                 -  How does the network optimally feedback toward
                    sustainable fertilizer application?
                Spatial granularity: 10s of meters to cm...
                Remote sensing, stationary and mobile nodes  (e.g.,
                distributed soil pylons, autonomous tractor-mounted
                sensors, aerial NIMS devices)
                Data interpolation, network calibration, and forecasting
                using detailed computational models
                 Nitrate
                 sensor
                 mimicking
                 plant root
                 fibers
        Geostatistical realization
        of soil properties
                                                                          Courtesy of Tom Harmon
                                                             SC  UCR  CALTECH CSU UC MERCEC
             CENTER FOR EMBEDDED NETWORKED
                             Plankton dynamics in marine environments
                                           Spatial and
                                           temporal
                                           distributions of
                                           harmful alga
                                           blooms (red,
                                           green, brown
                                           tides) in
                                           marine coastal
                                           ecosystems
                                          Experimental and
                                          observational studies of
                                          chemical, physical and
                                          biolgical features
                                          promoting bloom events
                                                       •UCLA USC  UCR  CALTECH CSU UC MERGED
             CENTER FOR EMBEDDED NETWORKED SENSIN
          Dr. Deborah Estrin
                                      1-4

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
                                                Nanotechnology, Biotechnology, and
                                                 Information Technology Workshop
                              Important Challenges for  EPA Applications
                               Robust, portable, self
                                configuring systems


                            Embeddable sensor devices
                                for specific species,
                                sensitivity, longevity


                                   Data Integrity,
                                    Calibration
                                     Multiscale
                                    Data Fusion
                                                       JC    c"       -       >- DC '- ; -~c ~"
             CENTER FOR EMBEDDED NETWORKED SENSIN
                                            Systems  Challenges
               Low-Power Platforms
                                    Key Constraints:
             Software,
Embeddable   Protocols
  Sensors
               Target Apps:
                                        Energy awareness
                                         and conservation


                                      Scaling and adaptation
                                       to variable resources
                                            and stimuli


                                           Autonomous,
                                           disconnected
                                             operation
                           Data Integrity given
                             sensing channel
                                uncertainty
InformationTechnolo
gy Research:

• Self configuring
systems for autonomy
in dynamic, irregular
environments

• In Network
Collaborative signal
processing and Event
Detection for Scaling in
time and space

• Exploiting
Heterogeneous
Systems w/ Mobility
                                                                    • Multi-mode, multi-
                                                                    scale data fusion for
                                                                    tasking, interpretation,
                                                       ..JLA  USC UCR CALTECH CSU UC MERCECl
          Dr. Deborah Estrin
                                                                                      1-5

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                               Heterogeneous Sensor Systems Needed
                  Spatially distributed static nodes
                      Allows simultaneous sampling across study
                      volume (dense in time, but possibly sparse in
                      space)
                      Limited energy and sampling rate
                  Articulated Nodes
                      Provide greater functionality for sensors,
                      communications
                  Nodes with infrastructure-based mobility:
                  Networked Info-Mechanical Systems (NIMS)
                      Sensor diversity: location, type, duration
                      Allows dense sampling across transect (dense
                      spatially, but possibly sparse in time)
                      Adaptive provision of resources (sensors, energy,
                      communication)
                      Enable adaptive, fidelity-driven, 3-D sampling
                      and sample collection
                                                                 IJCR  CALTEC  Cc_  DC MERCEC
                             Application-Driven (not Application-Specific)
                                         Common System Software
              Reusable, Modular, Flexible, Well-characterized Services/Tools :
                 •  Routing and Reliable transport
                 •  Time synchronization, Localization, Self-Test, Energy Harvesting
                 •  In Network Processing: Triggering, Tasking, Fault detection, Sample Collection
                 •  Programming abstractions, tools
                 •  Development, simulation, testing, debugging
                                                       •UCLA  USC  UCR CALTECH CSU UC MERGED
             CENTER FOR EMBEDDED NETWORKED SENSIN
          Dr. Deborah Estrin
                                       I-C

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                                      Embeddable Sensor developments
                  Environmentally robust sensors
                  (stationary and mobile deployment)
                  Initial emphasis or chemical species
                  (ionic)
                   - specifically nitrate
               •   Achievements and timeline
                   - nitrate Ion Selective Electrode,
                     demonstrate scaleability
                   - higher performance amperometric
                     nitrate sensor (Silver working electrode
                     sensitive for nitrate; Requires microfluidics)
                   - general ion separation/identification
                     capabilities (ion liquid
                     chromotography-on-a-chip)
               •   Transitioning to gas/atmospheric project:
                  CO2

               (* Judy, Harmon, Ho, Tai)
      ...the river is receiving
      excessive nutrients from
      adjacent qroundwater (not
      from surface runoff, not
      from atmospheric
      deposition)..."
                                                        •UCLA USC  UCR CALTECH CSU  UC MERCED
             CENTER FOR EMBEDDED NETWORKED SENSING
                                                 Data  Integrity:
                                    How will we monitor the monitors?
                                                                               .
                                                        •UCLA USC  UCR CALTECH CSU  UC MERGED
             CENTER FOR EMBEDDED NETWORKED SENSIN
          Dr. Deborah Estrin
                                       1-7

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                          Data integrity in sensor networks:  multilevel calibration
                                               Bench-top calibration
                                               Pilot deployment
                                               -  develop in situ calibration protocol
                                               -  characterize longevity, degradation
                                               Early in the deployment
                                               -  Take advantage of the sensors' integrity
                                               -  Calibrate model (distributed parameters)
                                               -  Integrate DAQ with simulator to accelerate process
                                               Later (as sensors become suspect)
                                               -  Reverse the process
                                               -  Let the network identity bad sensors: Self-Test
                                               Incorporate uncertainty into the process
                                                        UCLA Ejj
-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
       Nanotechnology, Biotechnology, and
        Information Technology Workshop
                            Multiscale Observation and Fusion: Example, Region?
                            (or greater) scale to local scale
                                                           Satellite, airborne remote sensing data
                                                           sets at regular time intervals
                                                           coupled to regional-scale "backbone"
                                                           sensor network for ground-based
                                                           observations
                                                           fusion, interpolation tools based on
                                                           large-scale computational models
                                          Example: identification of invasive
                                          riparian species using HyMap
                                          (airborne hyperspectral scanning)
             CENTER FOR EMBEDD
                               images from Susan Ustin, UC Davis
                          NEON
                     "NEON will transform ecological
                     research by enabling studies on
                     major environmental challenges at
                     regional to continental scales.
                     Scientists and engineers will use
                     NEON to conduct real-time
                     ecological studies spanning all
                     levels of biological organization
                     and temporal and geographical
                     scales.
                                                                     JK
                                                                    : **
   •Biogeochemical cycles
   •Biodiversity & ecosystem functioning
   •Climate change
   •Freshwater resources
            (especially linkage to land)
   •Infectious diseases
   •Land use change
   •Land use change and
   •Material flux or processing


•UCLA USC UCR CALTECH CSU UC MERCED
                           EDDED NETWORKED SENSING
          Dr. Deborah Estrin
                                              1-9

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                            Embedded Sensor Networks for NEON
            CENTER FOR EMBEDDED NETWORKED SENSIN
                                       HER
                                       Collaborative Large-scale Engi
                                       for Environmental Research
   ng Analysis Network
                                    California regional effort
               A multiscale approach - San
               Joaquin River Basin:  Water
               quality observation and
               forecasting—Sierra snowpack
               to San Franciso Bay
               Academics:  UC Merced,
               UCLA, UCD, UCR, Caltech
               Govt Agencies: LLNL, LBNL,
               USSR, USGS, NPS, CA
               DWR
                                                 •UCLA USC UCR CALTECH CSU UC MERGED
            CENTER FOR EMBEDDED NETWORKED SENSIN
         Dr. Deborah Estrin
                                  1-10

-------
 APPENDIX I - SLIDE PRESENTATION
Information Technology — Sensor Networks
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                                   Broad Relevance to Global  Issues
               Theatre, Film,
                  Television

             Global Climate
             Change


                Water
                Quality

             Early Warning,
             Crisis Response
                                                                     Security
            Precision
            Agriculture

         Public
         Health
              Coral Reef

         Global Seismic
         Grids/Facilities
                                                     ' UCLA  USC  UCR CALTECH CSU UC MERCEC
             CENTER FOR EMBEDDED NETWORKED SENSIN
                                        For Further Investigation
                   Center for Embedded Networked Sensing,
                   http://cens.ucla.edu
                   NSF Workshops including Sensors for
                   Environmental Observatories,
                   http://www.wtec.orq/seo/seo6.htm
                   Biosensing overview,
                   http://www.wtec.org/biosensing/proceedings/
                   National Ecological Observatory Network,
                   http://neoninc.org
                   TlnyOS and Mote platforms: UC Berkeley, Intel,
                   Crossbow, Sensicast, Dust Networks, Ember
                   Principles of Embedded Networked Systems
                   Design. Gregory J. Pottie and William J. Kaiser,
                   Cambridge University Press, Spring 2005
                                                     •UCLA  USC  UCR CALTECH CSU UC MERGED
             CENTER FOR EMBEDDED NETWORKED SENSIN
          Dr. Deborah Estrin
                                    1-11

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Information Technology (IT)
                   Implications for Future Science at EPA


                 Gregory J. McRae

                 MIT-Chemical Engineering
             Information/Analysis/Knowledge/Outcomes!!
  Dr. Gregory McRae
                         J-l

-------
  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
     Nanotechnology, Biotechnology, and
      Information Technology Workshop
               The "Problem'
                  Real Risks
Perceived Risks
                                 Y
              How can IT help in the development of
              appropriate responses (science/ policy)
              and in managing the complexity?
               Outline of Presentation
                                 What is IT?
                                 How is EPA doing?
                                 What are new directions?
                                 Issues for workshop?
             Key Message: Advances in IT have the potential to
                         revolutionize how EPA might manage
                         environmental risks.
  Dr. Gregory McRae
                              J-2

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                             Nanotechnology, Biotechnology, and
                              Information Technology Workshop
                What do we mean by "IT"?
Computers (pc's->sc's)
Data bases/management
Communications
Sensors
Visualization
Algorithms
Audit for accountability
PEOPLE
                                              AN are needed to
                                              reduce the elapsed
                                              time to solutions
                                              and to get relevant
                                              science into the
                                              decision making
                                              process before
                                              decisions are
                                              made.
                 What are the driving forces for change?
                 Driving Force For Change - The Web
                     Essential Utilities
                    Water  Gas  Electricity
            66ource:MattSpathas, SENTRE Partners
                          The 4th Utility
                           Bandwidth
  Dr. Gregory McRae
                                                        J-3

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                       Nanotechnology, Biotechnology, and
                        Information Technology Workshop
                Driving Forces for Change - Optical Networks
                   o
                   a
                   i_
                   a>
                   a
                   a>
                   o
                   c
                   (0

                   I
                   a>
                   0.
                                         Optical Fiber
                                        (bits per second)
                                    (Doubling time 9 Months)
Silicon Computer Chips
 (Number of Transistors)
(Doubling time 18 Months)/
     Data Storage
  (bits per square inch)
(Doubling time 12 Months)
                                     234
                                   Number of Years
             Scientific American, January 2001
              Driving Forces for Change - Remote Access
                                             Two 6.5 Meter Telescopes at Las
                                             Campanas Observatory, Chile
  Dr. Gregory McRae
                                                     J-4

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                Driving Forces for Change - The Movies
             Routine Visualization of Complex Phenomena
  Dr. Gregory McRae
                           J-5

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 New Dimensions of Working in Teams
               Environmental Problems are Global in Extent
                           Exposure to Environmental Health Risks (World Bank)
  Dr. Gregory McRae
                            J-6

-------
  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
               Characteristics of Energy/Environment Problems
              • Complex
              • Multiple (often conflicting) objectives
              • Asymmetric information
              • Short decision cycles
              • Long analysis times
              • Few technically qualified people
            Can we "really" contribute to the policy process?
  Dr. Gregory McRae
                        J-7

-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
Three
1.
2.
3.
Questions - Air Quality Issues

What has been the hourly 03 concentration
over Washington for the last 10 years?
Can we detect the impact of emissions
controls in a statistically meaningful
manner?
What is the most cost-effective way to
improve air quality in the North East?
Can we answer these questions and, even
importantly, in time scales compatible with
of regulatory decision making processes?
more
needs
Biological Data
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gggtactgcc
- Genetic Code
caggctatgc
ctgggggccc
ttcccagaac
tggccacaaa
gaggtgggtg
ctgcagcccc
gccgaggcca
tgagtgggtg
gggattggcg
gttaaggatc
ctctgaccgg
acacctctac
cacgctccac
cttgtttccc
cgctgcgtaa
gctacatgct
acgaacccct
tgac
agat
tcca
cacaat
aggctt
aggtca
gtattctgct
ttttgtgcat
cactgaagag
aggc
tgtc
gtgt
tggc
aagc
ctgc
ttcc
aacc
ggct
actg
gcag
cttggc
aagcat
cttggg
cttgcc
tccata
acttcc
atcctg
cttgta
gcacga
ccgttt
ccgcac
Sus scrota agouti -






gttgctga
ggcccccc
gc
tg
gtgcgggcag
tgtatgag
gggtggtt
gacaactg
agaggtaa
cc
ct
ca
ca
caacctctat
agcgctggga
atcccttc
ag
tgctctgggg
tgaccccc
ccttgatc
tc
tg
ggtgctagat
atcctgtc
cttcaacg
ctagctgg
related

tg
cc
cc
tgtgccctac
gagggtatcg
gagtgggttg
ctttcttccc
gccctcacat
gaacgggcag
gctcagggaa
ctgtgcttgg
cgtggtttca
cttaggctga
tgaccaaaaa
acccggggcg
gcgcattcca
cctgaaggac
ggacaccagg
ttctgctact
agccaatgtc
protein gene

MIT
  Dr. Gregory McRae
                              J-8

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Sequence Databases - www.ncbi.nlm.nih.gov
                 | Nucleotlde Databases
                 Domains
                 3D Oon
                 Taonfny

                 I Genome Databases

                 Genomes      I '"~i.=-i r-1


                 | Expression Databases

                 £E2        SAGE
                                              Growth of GenBank
                                           There are approximately 28,507,990,166 bases in
                                           22,318,883 sequence records as of January 2003
                   Why has Bioinformatics been so successful?
                      An organized community with roadmaps
                      Resources (NIH, NSF, DoE,...)
                      New people (Fellowships,...)
                      Multidisciplinary (Math, Eng, CS, Bio, Phys, Chem,...)
                      Focused on systems integration and leverage of community input
                       - Databases (GenBank,...)
                       - Algorithms (Matching,...)
                       - Instrumentation (Microarrays,...)
                       - Linking of private/public data  bases
                       - Multi-scale integration (Genomes to life,...)
                      Standards for representation of chemistry (ATGC, proteins,....)
                 IT has been a critical enabling element of
                 success of genome project and has
                 facilitated the emergence of new sciences
  Dr. Gregory McRae
                               J-9

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                              Nanotechnology, Biotechnology, and
                               Information Technology Workshop
                  PM-, c Controls - How is soot formed?
                     •2.5
                               COx
                  of
                                                       ccc
                                                                 ca
»T    _x
CO    Or
                                            co

                Challenges - Integration of Diverse Databases
                   Thermophysical properties
                   - JANAF
                   - NIST
                   - CHEMKIN
                   - DECHEMA
                   - Group contribution, QM


                   Kinetics
                   - NASA
                   - Bielstein
                   - DECHEMA


                   Species and Mechanism
                   - LLNL
                   - Literature, by-hand, GRI,...
                   - Reaction path generation

                   Etc.
                          1.  Not integrated
                          2.  Do not give useful
                             uncertainty estimates

                          3.  Varying levels of
                             documentation

                          4.  Few links to the
                             provenance of data

                          5.  How to handle different
                             data quality
                          6.  Few organized
                             experimental  data
                             bases for evaluation
  Dr. Gregory McRae
                                                          MO

-------
  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
            What could we do with IT? -Some Examples
                  SAB
                (Sciences)
                • Physics
                • Chemistry
                • Toxicology
 Risk Reduction
 (Engineering)
Emissions controls
Cost-effective design
Case studies
                    Compliance assessment
                    Avoiding problems in the first place
                    Win-Win control strategies
                    Prioritization of resources
                    Etc.
                Compliance Assessment:

                   How can we detect if
                   environmental controls have
                   been effective?
  Dr. Gregory McRae
                       Ml

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                                Nanotechnology, Biotechnology, and
                                 Information Technology Workshop
               One Environmental Goal - Meet Standards
                          Emissions
                      Compliance
                      Assessment
                 Deterministic Control Strategy Design
                                     Minimize cost of controls
                                     - Subject to meeting standards
                                     Maximize air quality
                                     - Subject to budget constraint
                                     Minimize exposure to pollution
                                     - Subject to limits
                                     Minimize risk of exceedances
                                     - Subject to fluctuations
                                     Etc.
mm  f(E,x,t)

s.t.  g(E,x,t) = 0

    h(E,x,t)<0
  Dr. Gregory McRae
                                                            J-12

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                Nanotechnology, Biotechnology, and
                Information Technology Workshop
               Compliance Assessment -Inverse Modeling
              Forward Modeling  (M:{E,M,C}->AQ)
              Inputs
Air Quality
 Mode I (M)
                              Air Quality
             Inverse Modeling (M 1:AQ->{E,M,C})

             Bounds
  Inverse
Model (W1)
                              Measurements
               Bayes Theorem -InverseProbability
                                              y)p(y)
           Prior Knowledge p(0)'
                   Posterior p(0|y)
p(91 y) =
                         P(y I Q)P(0)
                             p(y)
             Posterior
            Distribution
           a  p(y 10)p(0)

           	1
            Likelihood     Prior
            Function  Distribution
                          = \P(6)p(y\6)dd
  Dr. Gregory McRae
                                        J-13

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                                Nanotechnology, Biotechnology, and
                                Information Technology Workshop
                 Moving instrumentation to 21st Century
                  $ 300,000
                                 $10
            Source: ANL and MIT
              Schools as a Source for Data Intensive Science?
               Students enrolled in grades K-12
               Schools
               Teachers
                                      6,000,000
                                          9,000
                                        300,000
                                        200,000
Student per Internet-connected computer      7:1
               Classrooms with Internet access
                    Schools as sensor platforms for
                       - Air Pollution
                       - Water Quality and Quantity
                       - Seismic Activity
                       - Health of the Population
  Dr. Gregory McRae
                                                          J-14

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
      Nanotechnology, Biotechnology, and
       Information Technology Workshop
                Potential for Dramatic Increase in Coverage
                 US EPA PAMS Sites
                       PAMS (Photochemical
                       Assessment Monitoring Station)
              11 PAMS/SLAMS
              Monitors in 2001
             (CO, NO2, O3, SO2, PB, PM10)

             California Air Resources Board !
Schools in Los Angeles
Unified School District
                                    06
                                    rNifiyi
                                    jij-. 5
                                    •-I,-,- -:i •
                                    "bull 'f-\
                                    PFvERI.1 ' -ill : i.lhiRFI
                                    --.-• •'-' RNE iLEMIN'!
                                    •in; I-,' '
                                    -..' N RE J-ORj OR
                                    SF'.'-R!' ' -ill :
                                    LA
                                    in.-'i n
                                    JJU
                                    ""•• -i PE; ;FOR: PR
                                    BEVERLY HiLLS
             Avoiding Problems in the first place:

                Can we use IT to anticipate
                potential impacts of products
                before they are introduced?
  Dr. Gregory McRae
                                J-15

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
            Choice of New Chemicals - Cleaning CVD Reactors
e.g. F2 vs.
NF3, Ar,
N2
-f
NF3 Cleaning Process
RF Power
1
Plasma
Generator
J
F, NF, NF2, Ar
N2, F-, NF+ ...
in the Fab
CVD
Chamber


SiF4, F2, N2' Ar.

Downstream
Treatment


32
Why are Technology Choices Complex?
Exai
nple: Choosing a chamber cleaning gas (NF3 vs.
Decision Criteria
Fluorine usage rate at
the same etch rate
(mole/min)
Cost/mole of Fluorine
LCA Global Warming
Effect (kg CO2
equivalent/kg)
Toxicity LC50 (ppm)
NF3
0.15
$6
3.3
6700
F2
0.17
$0.8
2.4
180
Data
Mechanism
and
properties
Economics
Environment
LCA
Health
OHSHA

F2?)
The Problem: How to choose between technologies
- When there are conflicting decision criteria
- Many uncertainties r
  Dr. Gregory McRae
                           J-16

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   APPENDIX J - SLIDE  PRESENTATION
Information Technology — Large Scale Computing
                          Nanotechnology, Biotechnology, and
                           Information Technology Workshop
Why we need to solve this problem!!
Indust
Emerc
33
ry recognition of need
"...There is a critical need for an integrated way to
evaluate and qualify environmental impact of
process, chemicals, and process equipment..."
- ITRS, 2001 Edition, Environmental, Safety, and Health
jing Driving forces for Change
"...The European Commission Integrated Product
Policy (IPP) will look at aM stages of a product's
life cycle from cradle to grave... we are calling on
industry to bring IPP to life"
-- M. Wallstrb'm, EU Environment Commissioner
Press release 18th June 2003

MIT
               MIT Environmental Evaluation Model
                 Design
                 Decisions
                       Process
                        Model
                    Alternative Designs
Flow Rates
  Products
 Byproducts
  Chemical
   Energy
   Water
   Waste
                                 Yield
                               ^Process Time
                            Environmental Properties
                              Chemical Properties
                              Exposure Properties
  Dr. Gregory McRae
                                                           J-17

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                Nanotechnology, Biotechnology, and
                 Information Technology Workshop
                  Importance of Considering Multi-Boundaries
                             Upstream
                  Framework of Decision-Making Process
                     Generate new
                     alternatives
Refine model, collect more
data, increase data accuracy.
                                  Economic! Process
                                  Impact I Model
                                   Model
  Dr. Gregory McRae
                                             J-18

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                                Nanotechnology, Biotechnology, and
                                Information Technology Workshop
                 Risk Management -Decision Uncertainty

                 Global warming potential (GWP)
NF,
ih
25% 75%
H
* 50%
I





95%
                                             I 1 I
                                             15%
                                          m
                   0 0.17   0.5
                   GWP of Cleaning Processes
                   (kg CO2 equivalent)
                                          0 1 23 45 67  20 21 22 23 24
                            Relative Ratio of GWP of NF3
                            and F2 Cleaning Processes
                 There is a 85% likelihood that the F2 has a lower a
                 global warming impact than the NF3 cleaning.
                 Ethanol versus Methanol
  Dr. Gregory McRae
                                                          J-19

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
Some Background
Methanol
(Oil/Gas)
NOX, CO
CH3OH,
. 	 ^ HCHO,...
Formaldehyde is
a carcinogen!!
39
Ethanol
(Biomass)
NOX, CO
C2H5OH,
Crl^CrlO/iii
^
MIT

Atmospheric Chemistry of Methanol
CH3OH + OH^"-^H2O + HCHO + HO2
HCHO + hv^"-^CO + 2HO2
_k m_ *i[M>2]
1 ^ L"JJ k3[NO]
^| 3NO^3NO2
« MIT
  Dr. Gregory McRae
                             J-20

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  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                             Nanotechnology, Biotechnology, and
                             Information Technology Workshop
Atmospheric Chemistry of Ethanol
CH3CH2OH + OH -> 	 > H2O + CH3CHO + HO2
HO2 + NO -> NO2 + OH
CH3CHO + hv^
\
/
>
/
CH3O2+NO-
3 2
HO2+NO^^
41
^C//4+CO
^ C//3O2 + H2O
->NO2+CH3O
HCHO + HO2
NO2+OH
AND
MIT
               Atmospheric Chemistry of Ethanol (Cont.)
    CH3CHO
      CH3CO
CH3C(O)O2
                                 CH3CO + H2O
                                CH3C(O)O2
                                    CH3C(O)O2NO2 + M
                                 Peroxyacety I nitrate (PAN)
              More NO to NO2 conversions than methanol (5)
              Formaldehyde is a photo-oxidation product
              Chemistry produces PAN, a phyto-toxicant
  Dr. Gregory McRae
                                                    J-21

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  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
              Nanotechnology, Biotechnology, and
               Information Technology Workshop
            Win-Win Control Strategies:

              How to view environment as an
              objective, not as a constraint, in
              design optimization?
             Motivating Problem - Improving oldprocesses
               C2H4O
                      • World capacity 22 billion Ibs/year
                      • Very low margins
                      • > 50 year old process
  Ethylene     Coolants (29%)
  Glycols  [_ Polyesters (32%)
- Surfactants (13%)

 Glycol ethers (7%)
 Ethanolamines (6%)
 Other (13%)
  Dr. Gregory McRae
                                     J-22

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
             Nanotechnology, Biotechnology, and
              Information Technology Workshop
              Win - Wi n Desig n - More product and less CO2
                                  Product
                                       2CO
                      2H2O
                                 Climate Problem
                 Improving yield/selectivity can improve
                 both profit and reduce climatic impacts
                Solution Strategy - Multiscale Engineering
                 Catalyst
                 Surface
                O(nm)
Reactor
 Tube
O(cm)
            Many reactions / species   Mass transfer resistances
            Surface thermodynamics   Packing in homogeneity
0(m)
              Bundle of many tubes and
             The need for shell flow details
  Dr. Gregory McRae
                                       J-23

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  APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
                           Nanotechnology, Biotechnology, and
                            Information Technology Workshop
            Prioritization of Resources:
              There are lots of uncertainties, the
              challenge is to identify those that
              contribute to uncertainties in
              outcomes?
              A More Complicated (Realistic) Viewpoint

min  f(E,x,t)
s.t.   g(E,x,t) = Q
     h(E, x,t)
-------
   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
       Nanotechnology, Biotechnology, and
        Information Technology Workshop
              Valuing Decisions - NPV, Option Pricing,...
                       Probability
                      of achieving
                     environmental
                         quality
Strategy B
                                              Strategy A
                                               Environmental Quality
               Which control strategy(s) would you choose?
               Problem: Identifying Critical Parameters
                   Photochemical Reaction Mechanism
               ~1  NO2 + hv -> NO + O

                3  O3 + NO -> NO2 + O2
                4  HCHO + hv-> 2HO2. + CO
                5  HCHO + hv -> H2 + CO
                6  HCHO + OH. -> HO2. + CO + H2O
                7  HO2. + NO -> NO2 + OH.
                S  OH. + NO2 -> HNO,
                Predicted
                03 + 1 std
                                               0   20  40
                                                               00  120
                                                      Time (min)
                           Contributions from different parameters to
                           uncertainty in predicted ozone levels
  Dr. Gregory McRae
                                   J-25

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
Nanotechnology, Biotechnology, and
 Information Technology Workshop
               What is Driving Uncertainties in Outcomes?
                           Parameters (porosity, permeability, saturation,
                                       market economics,...)
               Black Oil Model
                                     Water
                                     Oil
                                       •{**)-*{•*•}•»
                                     Gas
                          Performance Drivers
                                   ..^Permeability
                                   { Porosity
                                     Water saturation
                 Effect of New Information on Risk
                   NPV
                ($ Million)
                                       Years
                   Reduced Risk and Increase in average NPV
  Dr. Gregory McRae
                           J-26

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   APPENDIX J - SLIDE PRESENTATION
Information Technology — Large Scale Computing
  Nanotechnology, Biotechnology, and
   Information Technology Workshop
               Conclusions/Workshop Questions

               • IT is a critical enabling resource, does
                 EPA need a CTO/CIO?
               • How to improve access to data bases
                 used for decision making?
               • Critical need for multimedia integration of
                 databases/models (MTBE!!)
               • How to get more science into the control
                 strategy design process?
               • Most critical issue is where will the
                 people come from?
            MIT
            Chemical Engineering
                            Course 10
TSW
                          Contact Address:
                           Department of Chemical Engineering
                           Room 66-372
                           Massachusetts Institute of Technology
                           Cambridge, MA 02139
                           (617)2536564
                           (617) 258 1925 (fax)
                           mcrae@mit.edu (email)
                           http://www. mit. edu
  Dr. Gregory McRae
                          J-27

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                Converging Technologies
                            (NBIC)
             William Sims Bainbridge, Ph.D.
              National Science Foundation
                           NBIC =
              Nanotechnology
              Biotechnology
              Information Technology
              Cognitive Science - new technologies based on
              the convergence of computer science,
              psychology, neuroscience, philosophy,
              anthropology, economics, sociology, etc.
Dr. William Bainbridge
                         J-l

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                   The Meaning of NBIC:
                Based on the unity of nature at the nanoscale
                A potential successor to the National
                Nanotechnology Initiative, and to the
                Information Technology Research  Initiative
                Not an official government (or NSF) activity,
                but an exploratory movement of scientists and
                engineers
                Arising when the unification of science has
                become possible through the use of
                transforming tools
                A natural extension of work on the societal
                implications of nanotechnology
                Unification of Technology
              Manuel Castells writes, "Technological convergence
              increasingly extends to growing interdependence
              between the biological and micro-electronics
              revolutions, both materially and methodologically.
              ... Nanotechnology may allow sending tiny
              microprocessors into the systems of living
              organisms, including humans." (Castells, Manuel.
              2000. The Rise of the Network Society.  Oxford:
              Blackwell, p. 72.)
Dr. William Bainbridge
                            J-2

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                   Unification of Science
             In his influential book, Consilience, Edward O.
             Wilson wrote about the rapid unification of
             scientific knowledge that is taking place today, and
             he wondered whether the natural sciences would be
             able to unite with the humanities and religion that
             traditionally have claimed to understand humanity
             itself.  (Wilson, Edward O. 1998.  Consilience: The
             Unity of Knowledge. New York: Knopf.)
                        Hatching an Idea

                Converging Technologies
                for Improving Human Performance:
                Nanotechnology, Biotechnology,
                Information Technology and Cognitive Science
                NSF/DOC-sponsored report
                                 Conference at NSF,
                                 December 3-4, 2001
                http://www.wtec.org/ConvergingTechnologies/
Dr. William Bainbridge
                            J-3

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
Li




aunching a Movement


© o

1 NanoiechnolOFt. Biotetlmotom. Information Teclmolon. and Conmive Science 1
NBIC CONVERGENCE 2003
Converging Technologies for
Improving Human Performance
• February 5-7, 2003 • UCLA • Los Angeles. CA •






7
                         First Publications
                Mihail C. Roco and William Sims Bainbridge (eds.) 2003.
                Converging Technologies for Improving Human
                Performance. Dordrecht, Netherlands: Kluwer.
                Mihail C. Roco and Carlo D. Montemagno (eds.) 2004.  The
                Coevolution of Human Potential and Converging
                Technologies. New York: New York Academy of Sciences.
                (Annals of the New York Academy of Sciences, Vol. 1013)
Dr. William Bainbridge
                               J-4

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                            Next Steps
                         NBIC Convergence, New York City,
                         February 26-27, 2004 (book in
                         preparation, edited by William Sims
                         Bainbridge and Mihail C. Roco.)

                         Converging Technologies
                         Kailua-Kona, Hawaii
                         February 23-25, 2005
                         http://www.biztechcomm.com/
                         (book expected, edited by
                         Bainbridge, Montemagno & Roco)
                  The  NBIC Tetrahedron
                                 Naiio
                       Cogno
                               Info
                        Nanotechnology
                         Biotechnology
                    Information Technology
                        Cognitive Science
                                                     10
Dr. William Bainbridge
                          J-5

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Principles of
                Convergence
                 Convergence is based on:
                 1. material unity of nature at the nanoscale
                 2. technology integration from the nanoscale
                 3. key transforming tools for NBIC
                 4. concept of reality as closely coupled
                    complex, hierarchical systems
                 5. goal to improve human performance
             Application
                 Areas
             •Expand Human Cognition & Communication
             •Improve Human Health & Physical Capabilities
             •Enhance Group & Societal Outcomes
             •Strengthen National Security & Competitiveness
             •Unify Science & Education
Dr. William Bainbridge
                           J-6

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                   One-way Convergence
             Developments in one field are applied to another.

             E.g.: Nanotechnology allows Moore's Law to continue
                  in production of ever smaller, faster, and
                  cheaper microelectronic components - enabling
                  continued progress in Information Technology.
             An end to Moore's Law could mean a shift to massive
               parallel computing, but cost and technical
               challenges have limited the use of parallel systems.
             If 1C chips become ordinary commodities, nations
            k    with low labor costs may mass produce them
                thereby destroying American (etc.) industries.
                      Mutual Convergence
            Scientific theories and models are applied across
            many different fields, facilitating exchange.
             Judith Klein-Seetharaman and Raj Reddy:
             "Biological Language Modeling"

             William Sims Bainbridge:
             "Evolution of Semantic Systems"
                                                        14
Dr. William Bainbridge
                            J-7

-------
APPENDIX K - SLIDE PRESENTATION
        Converging Technologies
               Nanotechnology, Biotechnology, and
               Information Technology Workshop
                                             Information
                                             Technology
                                                 Grants
               NSF Awards 0225656, 0225636, 0225609, 0225607:
               "Computational Learning and Discovery in Biological
               Sequence, Structure and Function Mapping" estimated total:
               $8,840,267; Carnegie-Mellon, U Pittsburgh, MIT, Boston U

               Computer scientists, together with biological chemists will
               collaborate using statistical and computational tools and
               methods that the computer scientists have been developing for
               dealing with human language to better understand the function
               of proteins.
              Evolution
                                      Biology:
                       Language:
                                     Multiple genome
                                      sequences
                                    Expression, folding,
                                   structure, function and
                                    activity of proteins
                                               ^rMappjn^)/
Understand complex
biological systems
                  Evolutionary biology to semantic evolution:
                    Taxonomy: Linnaean genus-species system,
                     cladistics, numerical taxonomy
                    Processes: Gene, Sexuality, Transaction, Alleles,
                      Natural Selection, Species, Stratigraphy, Catastrophism,
                      Van Valen's Law, Character Displacement, Allopatric
                      Speciation, The Cope-Stanley Law, Exaptation
                                                                  16
Dr. William Bainbridge

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
               Depth
                with
              Breadth
                                          Similar Tcols, Similar Material
                                         .   Different Qbjectcwas
                   "Combining depth with breadth in NBIC
                   education and research of various groups."

                   "Nanotechnology offers hope of depth plus
                   breadth"
               A     (W. M. Tolles: "Breadth, Depth, and
                      Academic Nano-Niches" - 1st report)
                                                           17
                     Sustaining Progress
               Has progress stalled in aviation and spaceflight,
               energy production, artificial intelligence, social
               and behavioral science, health and longevity?
                                    2IH-M2S
               The Future
                   Today
              Tkt 2nd Indinlrul
                KtvohltiuA
               IMS
   Succession of
 sigmoid (logistic)
 curves of progress
 (Newt Gingerich:
     "Age of
   Transitions")
Dr. William Bainbridge
                             J-9

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
     Nanotechnology, Biotechnology, and
     Information Technology Workshop
                      Transforming Tools
              Opportunity for science & technology convergence
              based on shared methodologies (e.g. mathematics,
              computation, nanoscale observation and
              experimentation, etc.) & theories (e.g. hierarchical
              structures, complex systems, evolution, etc.)
                 Analogous
               structures in the
                different fields
               (James Canton:
              •'Global Futures")
                                 Computers

               Biotech
N,
Bits     Genes
  21st Century
  Architecture
eurons    Atoms
                                 Networks
                                                       Nanotech
                       Illustrative Application
               Comfortable, wearable sensors and computers will
               enhance every person's awareness of his or her
               health condition, environment, concerning potential
               hazards, local businesses, natural resources and
               chemical pollutants.
               "Spatial Cognition
               and Converging
               Technologies"

                 (Reginald G.
                 Golledge)
                                                           20
Dr. William Bainbridge
                                 MO

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                        More  Applications
            National security will be greatly strengthened by light-weight
            information-rich war fighter systems, capable uninhabited
            combat vehicles, adaptable smart materials, invulnerable data
            networks, superior intelligence gathering systems, and effective
            measures against biological, chemical, radiological, and nuclear
            attacks.
             Agriculture and the food industry will greatly increase yields
             and reduce spoilage through networks of cheap, smart sensors
             that constantly monitor the condition and needs of plants,
             animals, and farm products.
             Becoming  Renaissance  People
             Formal education will be transformed by a
             unified but diverse curriculum based on a
             comprehensive, hierarchical intellectual
             paradigm for understanding the architecture
             of the physical world from the nanoscale through
             the cosmic scale.
                       Engineers, artists, architects, and designers
                          will experience tremendously expanded
                             creative abilities, both with a variety
                              of new tools and through improved
                              understanding of the wellsprings of
                                              human creativity.
                                                             22
Dr. William Bainbridge
                             Ml

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                            Discovering...
             ...new categories of materials, devices and systems for use in
             manufacture, construction, transportation, medicine, emerging
             technologies and scientific research.

             ...processes of the living cell, which is the most complex known
             form of matter - with nanoscale components.

             ...principles of advanced sensory, computational and
             communications systems integrating diverse
             components into a ubiquitous, global network.

             ...structure, function, and occasional
             dysfunction of intelligent systems,
             most importantly the human mind.
                 Social and  Ethical  Principles
            ....evolving socio-cultural context in which
            convergent research is funded
            ....societal needs that technology may satisfy
            ....popular misconceptions that science
            education will have to overcome
            ....infection of one field by issues from a
            different convergent field, e.g.: nano from bio
                         A     A
                                                            24
Dr. William Bainbridge
                             J-12

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
  Nanotechnology, Biotechnology, and
  Information Technology Workshop
                 Oversight I
                See:
                "Societal Implications of
                Nanoscience and
                Nanotechnology"
                at:
              www.wtec.org/loyola/nano/societalimpact/nanosi.pdf
                                                             25
                            Oversight II
                Societal Implications of Nanoscience &
                      Nanotechnology It:
                    Maximizing Human Benefit
                 Report of the National Nanotechnology Initiative Workshop

 Second Report on
      Societal
 Implications to be
  Published Soon!
 (Mihail C. Roco &
   William Sims
Bainbridge, editors)
                                                             26
Dr. William Bainbridge
                               M:

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APPENDIX K - SLIDE PRESENTATION
       Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                 Improving Human Performance
               ...offering individuals and groups an increased
               range of attractive choices while preserving such
               fundamental values as privacy, safety, and moral
               responsibility
               ...substantially enhancing human mental,
               physical, and social abilities
               Technological civilization faces
               the very real danger of stasis or
               decline unless something can
               rejuvenate progress.
                                                          27
                            Unification
            Enhancement of human performance should serve the
            legitimate hopes of human beings, who in return will
            support the scientific and engineering work required
            to achieve technological convergence and the
            unification of science.
                                       Convergence conferences
                                       have envisioned the next
                                       20 years, but complete
                                       unification of science may
                                       reQuire the entire 2 1st
                                       century.
               © 2003, www.XLR8TV.com
Dr. William Bainbridge
                            J-14

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
             Examples of NSF  NBIC Grants
            "Active Sensor Networks with Applications in Marine
            Microorganism Monitoring" (0121141, Requicha,
            USC). For monitoring microbes in the ocean or in
            water supplies: distributed network-coordinated
            nanorobots "to investigate the causal relationships
            between environmental conditions and micro-
            organisms."

            "Pattern Recognition for Ecological Science and
            Environmental Monitoring" (0326052, Dietterich,
            Oregon State) Computer vision system designed to
            recognize and count insects - a new tool for studies of
            biodiversity & water quality monitoring.         29
                More Examples of Grants
           "Interactive Software Systems for Expert-Assisted
           Image Analysis and Classification of Aquatic Particles"
           (0325937, Sieracki, Bigelow Lab; 0325167, Riseman, U.
           Massachusetts; 0325018, Benfield, LSU).  Computer
           vision, machine learning inspired by human cognition,
           to classify bacteria, plankton in ocean water.

           "Sustainable and Generalizable Technologies to
           Support Collaboration in Science" (0085951, Olson, U
           Michigan).  Studied online research collaboratories in:
           atmospheric science, behavioral neuroscience,
           biomedical informatics, computer science, earth
           science, engineering, genomics, and nanoscience.   30
Dr. William Bainbridge
                           J-15

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APPENDIX K - SLIDE PRESENTATION
      Converging Technologies
Nanotechnology, Biotechnology, and
 Information Technology Workshop
                        Converging
                        Technologies
              W. S Bainbridge: wbainbri@nsf.gov
                                                  31
Dr. William Bainbridge
                        J-16

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