GULF OF MEXICO DISSOLVED OXYGEN
  MODEL (GOMDOM) RESEARCH AND
QUALITY ASSURANCE PROJECT PLAN
                            Gulf of Mexico
                            Hypoxic Zone
Prepared by James J. Pauer1, Timothy J. Feist2, Amy M. Anstead3, Wilson Melendez4, Russell
              G. Kreis, Jr.1, and Kenneth R. Rygwelski1

   1USEPA; 2Trinity Engineering Associates, lnc.;3ICF International; 4CSC Corporation;

               U.S. Environmental Protection Agency
                Office of Research and Development
        National Health and Environmental Effects Research Laboratory
            Mid-Continent Ecology Division, Duluth, Minnesota
     Large Lakes and Rivers Forecasting Research Branch, Grosse lie, Michigan

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                                  FOREWORD
Over-enrichment of waterways by nutrients is a national and global issue and has subsequent
effects on freshwater, brackish, and  marine systems.   One  of  the  symptoms  of  nutrient
enrichment is hypoxia, such as that observed in  the Gulf of Mexico and is one of the largest
hypoxia zones observed on a worldwide basis.  It is incumbent on water quality managers to
protect and to identify appropriate management strategies to mitigate the impacts of  nutrient
stressors.  In the following research and quality assurance project plan, we provide a modeling
and forecasting approach which will aid managers in the decision-making process  for  abating
hypoxia impacts to the Gulf of Mexico.

This document has  been  developed  following  the  U.S.  Environmental Protection Agency
(USEPA)  Guidance for Quality Assurance Project Plans, EPA  QA/G-5 (USEPA, 2002a) and
USEPA Guidance  for Quality Assurance Project Plans for Modeling,  EPA QA/G-5M (USEPA,
2002b). However,  the document is a joint research plan and quality assurance project plan that
also incorporates  elements  of the USEPA, Office of Research and Development,  National
Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division (MED),
Guidelines for the Preparation of MED Research Plans (USEPA,  2000).  Additionally,  other
modeling  quality assurance guidance documents  have been consulted (USEPA,  1991; ASTM,
1992;  Richardson  et a/.,  2004; National Research  Council,  2007;  USEPA, 2008a, 2009).
Beyond being a prototypical, combined research  plan and quality assurance project plan, the
emphasis is on mathematical modeling.
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                                    NOTICE
The information  in this document has been obtained primarily through funding by the United
States Environmental Protection Agency (USEPA) under the auspices of the Office of Research
and  Development (ORD).   The  report has  been subjected  to  the  Agency's  peer and
administrative review, and  it  has been approved  for publication as a USEPA document.
Mention of  trade names  or  commercial  products  does not constitute  endorsement  or
recommendation for use.
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                                  ABSTRACT
An integrated high resolution mathematical modeling framework is being developed that will link
hydrodynamic,  atmospheric, and water quality models for the  northern Gulf of Mexico.  This
Research and  Quality Assurance Project Plan  primarily focuses on the  deterministic Gulf of
Mexico Dissolved Oxygen Model (GoMDOM). The GoMDOM models are similar in that they all
are derived from the LM3 Eutrophication model developed for Lake Michigan, but they differ in
spatial resolution and/or application. The other models are described only for the purposes of
understanding their inputs and linkages to the GoMDOM  models.  The GoMDOM models are
based on mass-balance principles and integrates multimedia nutrient inputs (primarily from the
atmosphere and the Mississippi and Atchafalaya Rivers) and ecosystem dynamics to establish
a forecasting capability for exploring management options to reduce the hypoxia zone.  The
GoMDOM models consist of a  coupled (eutrophication/dissolved oxygen  (DO) and sediment)
water quality model that is linked to an atmospheric model (Community Multi-scale Air Quality
(CMAQ)) model and are  driven  by a linked hydrodynamics model (EPACOM). The GoMDOM
model framework will be  calibrated and confirmed using cruise  data (2003 - 2007) specifically
collected for the modeling  effort along with other evaluated  project  and non-project data.
Uncertainty, sensitivity, and other statistical analyses will be performed to estimate the accuracy
of the water quality model predictions.  Finally, the 6km x 6 km gridded GoMDOM  model will be
applied to estimate the impact of several nutrient reduction scenarios on Gulf hypoxia, including
the allowable nutrient loads that would  reduce the five-year running average areal extent of the
hypoxic  zone to less than 5,000 km2 by 2015.  This effort  will assist managers in formulating a
strategy to achieve the goals specified in the Gulf of Mexico Action Plan.
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                            ACKNOWLEDGMENTS
The USEPA, Mid-Continent Ecology Division, Large Lakes and Rivers Forecasting Research
Branch wishes to acknowledge its partners and collaborators.

We are grateful to Bryon O. Griffith and Melanie Magee of the USEPA Gulf of Mexico Program
Office for encouragement and support during the planning phases of this project.  We thank the
USEPA Office of Water/ Office of Wetlands, Oceans, and Watersheds for shipboard sampling
allocations aboard the OSV Peter W. Anderson and the OSV Bold; as well as the crews of both
vessels.  Instrumental  in project sampling,  project  planning,  data analysis and interpretation
have been the staff of the USEPA, ORD Gulf  Ecology Division including Richard M.  Greene,
James Hagy, Janis Kurtz, John Lehrter, Michael Murrell, and Diane Yates.  We also wish to
thank staff from the USEPA Office of Water, USEPA Regions, and State personnel,  as well as
Leroy Anderson and Samuel Miller of the USEPA, ORD,  Mid-Continent Ecology Division for
their support during shipboard activities.

We would like to thank Robin Dennis and Ellen  Cooter of the USEPA, ORD,  National Exposure
Research Laboratory for their contributions in atmospheric modeling.  We wish to acknowledge
Robert Arnone and Dong-Shan Ko of the US Navy, Naval Research Laboratory for their efforts
in hydrodynamic modeling.  We wish to  posthumously recognize the contributions of Peter M.
Eldridge of the USEPA, ORD, Western Ecology Division in advancing our understanding of Gulf
of Mexico hypoxia and process modeling research.  Also, we  wish to thank the EPA staff and
their  support  contractor (Lockheed  Martin) at the  USEPA Environmental  Modeling  and
Visualization Laboratory for their contributions to model development and visualization.

We also wish to show our appreciation for other staff at the USEPA, ORD, MED, Large Lakes
Research Station who have supported the project  in numerous ways including: David  Miller
(USEPA),  Ronald  Rossmann (USEPA),  Mark  Rowe  (USEPA),  Xiaomi Zhang  (TEA), David
Griesmer (CSC), Kay Morrison (CSC), and Debra Caudill (ASRC).
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                            EXECUTIVE SUMMARY
Hypoxia and anoxia (low oxygen and oxygen depletion, respectively) are observed worldwide in
freshwater, brackish,  and saltwater  systems.   These  so called  "dead zones"  have been
observed and studied for well over one-half of a century.  They are primarily attributed to human
activity and land  use which have increased  nutrient  inputs  and advanced  the  onset  of
eutrophication.   Nutrient  stimulation of algal and  plant growth produces  large quantities  of
organic matter, and it is  the subsequent bacterial decomposition of the organic carbon that
imposes oxygen demand and depletion on the water column and underlying  sediment interface.
When  hypoxia  becomes extensive, vital socio-economic factors  such as recreation, food,
energy, transportation and industry can become impaired.

Hypoxia in the northern Gulf of Mexico is the largest such  zone in the U.S. and second largest in
the world.  Concern regarding the hypoxic zone size, duration and frequency centers  around
habitat alteration and impacts to various Gulf fisheries. Since the mid-1980s, hypoxia has been
documented and tracked in the Gulf, where it has been seasonally observed to be  as large as
22,000 km2.  Approximately 40% of the contiguous U.S., encompassing 31  States, is drained
through the Mississippi River basin and enters the Gulf of Mexico through  the Mississippi-
Atchafalaya complex.  Point and non-point sources contribute to high nutrient loads originating
from population centers,  farms,  and industry.   To  encourage nutrient loads reduction, the
Mississippi River Watershed/Gulf of Mexico  Nutrient Task Force,  through the Gulf Hypoxia
Action  Plan, has promoted an adaptive management approach together with a dual approach
for nitrogen and phosphorus reductions. However, with the many steps taken by Federal, State,
and local agencies, as well as landowners,  these activities have not resulted in a significant
reduction in the hypoxic zone.

The  Gulf  hypoxia modeling  framework  is designed  to  integrate  monitoring, condition
assessment,  diagnosis, and experimentation within  a mathematical modeling construct that
incorporates multimedia inputs,  environmental data,  and ecosystem dynamics to  establish a
forecasting capability.  The goal  of this collaborative effort is to develop a state-of-the-science,
mathematical modeling framework that will aid water resource managers in making scientifically
defensible  nutrient restoration decisions.  Specifically, the  model will  be applied to  estimate
several nutrient load reduction scenarios, including the nutrient loads that decrease the 5-year
year running  average size  of the zone to less than 5,000 km2 by the year 2015, a target
specified in the Gulf  of  Mexico Action Plan.   With nutrient  caps  established through an
integrated,  multimedia  modeling approach, it is anticipated that the size of the hypoxic zone can
be reduced and associated improvements will be realized in  habitat  and toward biological
resources that are balanced and  productive.

This Research and Quality Assurance Project Plan focuses on the following components of the
modeling project:

       Project management, objectives, and description; quality  objectives;  special training
       needs; and  documents and records management;

       Data generation and acquisition;

       Model construct,  coding, inputs, confirmation and corroboration,  sensitivity/uncertainty
       analysis, and application;
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      Assessment and oversight;



      Data validation and usability.
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                           LIST OF ABBREVIATIONS
ASRC
ASTM
CDF
CE-QUAL-ICM
CMAQ
CSC
CREM
CTM
1-D
3-D
DO
DQO
ECOM
EPA
EPACOM
EMVL
FIPS
FOIA
FRA
CIS
FRC
GoMDOM
GED
IAS
IASNFS
LLRFRB
LLRS
LM3
LM3-Eutro
LUMCON
MED
MM5
MODIS Aqua
NARA
NASA
NCAR
NCOM
NERL
NetCDF
NH3
NHEERL
NRC
NRL
NOAA
ORD
PCB
PDF
PDOM
POM
PRISM
QA
Artie Slope Regional Corporation
American Society for Testing and Materials
Common Data Format
US Army Corps of Engineers Three-Dimensional Water Quality model
Community Multi-scale Air Quality
Computer Sciences Corporation
Council for Regulatory Environmental Modeling
Chemical Transport Model (a global atmospheric chemical transport model)
One Dimensional
Three Dimensional
Dissolved Oxygen
Data Quality Objective
Estuarine, Coastal, and Ocean Model
Environmental Protection Agency
Coastal Ocean Model for the Northern Gulf of Mexico developed for EPA
USEPA Environmental Modeling and Visualization Laboratory
Federal Information Processing Standards
Freedom of Information Act
Federal Records Act
Geographical Information System
Federal Records Center
Gulf of Mexico Dissolved Oxygen Model
Gulf Ecology Division
Intra-Americas Sea Model
Intra-Americas Sea Ocean Nowcast/Forecast System
Large  Lakes and Rivers  Forecasting Research Branch
Large  Lakes Research Station
Lake Michigan Level 3 water quality model
Lake Michigan Level 3 water quality model - Eutrophication
Louisiana Universities Marine Consortium
Mid-Continent Ecology Division
A regional model for creating weather forecasts and climate projections
Moderate Resolution Imaging Spectroradiometer on the Aqua satellite
National Archives and Records Administration
National Aeronautical and Space Administration
National Center for Atmospheric Research
Navy Coastal Ocean Model
National Environmental Research Laboratory
Network Common Data Form
Ammonia
National Health and Environmental Effects Research Laboratory
National Research Council
Naval  Research Laboratory
National Oceanic and Atmospheric Administration
Office of Research and Development
Polychlorinated biphenyls
Portable Document Format file
Princeton Dynalysis Ocean Model
Princeton Ocean Model
Parameter Elevation Regression on Independent Slopes Model
Quality Assurance
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QAPP              Quality Assurance Project Plan
RQAPP             Research and Quality Assurance Project Plan
ROMS              Regional Ocean Model
QC                Quality Control
RCS               Revision Control System
SEAWIFS           Sea-viewing Wide Field -of-view Sensor
SOD               Sediment Oxygen Demand
SSWR              Safe and Sustainable Water Resources
TEA               Trinity Engineering Associates
URL               Uniform Resource Locator
USEPA             U.S. Environmental Protection Agency
USGS              U.S Geological Survey
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                 GROUP A: PROJECT MANAGEMENT
Signature
                    Date
Signature
                    Date
 A.1 Title and Approval Sheet

Research and Quality Assurance Project Plan Approvals - Mid-Continent Ecology
Division
Principal Investigator:
Russell G. Kreis, Jr.
Large Lakes and Rivers Forecasting
Research Branch

Branch Chief:
Russell G. Kreis, Jr.
Large Lakes and Rivers Forecasting
Research Branch

Administrative:
Dave Bolgrien,  Chair
Quality of Science Committee

Barbara Sheedy
Quality Assurance Manager

Janet R. Keough
Associate Director for Science

Carl Richards, Director
Mid-Continent Ecology Division

Health and Safety:
Eric S. Mead
Safety, Health and Environmental
Management

Animal Care and Use:
Michael D. Kan!, Chair
Animal Care and Use Committee
Signature
                    Date
Signature
                    Date
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 A.2 Contents

                          TABLE OF CONTENTS

FOREWORD	iii

NOTICE	iv

ABSTRACT	v

ACKNOWLEDGMENTS	vi
EXECUTIVE SUMMARY	vii

LIST OF ABBREVIATIONS	ix
GROUP A: PROJECT MANAGEMENT	 1
      A. 1 Title and Approval Sheet	1
      A.2 Contents	2
      A.3 Distribution List	4
      A.4 Project/Task Organization	5
      A.5 Problem Definition/Background	6
      A.6 Project/Task Description	9
      A.7 Quality Objectives and Criteria for Measurement Data	13
      A.8 Special Training Needs/Certification/Expertise	15
      A.9 Documents and Records	15
GROUPS: DATA GENERATION AND ACQUISITION	18
      B. 1 Model Formulation	18
      B.2 Model Coding	23
      B.3 Model Inputs	24
      B.4 Model Confirmation	26
      B.5 Model Calibration and Corroboration	27
      B.6 Model Sensitivity/Uncertainty Analysis	28
      B.7 Model Application	28
      B.8 Data Management	29

GROUP C: ASSESSMENT AND OVERSIGHT	31
      C. 1 Assessments and Response Actions	31
      C.2 Reports to Management	31

GROUP D: VALIDATION AND USABILITY	32
      D.1 Model Review	32
      D.2 Verification and Validation Methods	32
      D.3 Reconciliation with User Requirements	33

REFERENCES	34
FIGURES	41

TABLES	49
APPENDIX 1:  Conceptual Equations for Dissolved Oxygen and Sediment Diagenesis	52
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                               LIST OF FIGURES

Figure 1.  MED Gulf of Mexico Hypoxia Modeling Organization	41
Figure 2.  Overview of Project Clients and Collaborators (with lines of communication only)	42
Figure 3.  Gulf of Mexico Study Area	43
Figure 4.  Areal Extent of 2007 Hypoxic Zone	44
Figure 5.  Changes in Areal Extent of 1985-2008 Hypoxic Zone	45
Figure 6.  Annual Nitrate Load to the Gulf of Mexico	46
Figure 7.  Total Annual Phosphorus Load to the Gulf of Mexico	47
Figure 8.  Integrated, Multimedia Gulf of Mexico Modeling Framework	48
Figure 9.  Sediment-Water Interactions	48
                               LIST OF TABLES

Table 1. Overall Project Schedule	49
Table 2. List of Desired Field Measurements	50
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 A.3 Distribution List

The USEPA Gulf of Mexico Program Office in partnership with the USEPA Office of Research
and Development and USEPA Office of Water are building upon past efforts and have initiated
design plans for a framework that guides the science needed to address the hypoxia problem in
the Gulf of Mexico to meet the objectives of the Gulf Hypoxia Action Plan.  The distribution list
consists of those listed below and others to be determined.

Administrative/Management
Russell G. Kreis, Jr., Chief, Large Lakes and Rivers Forecasting Research Branch
Dave Bolgrien, Chair, Quality of Science Committee
Barbara Sheedy, Quality Assurance Manager
Janet R. Keough, Associate Director for Science
Carl Richards, Director, Mid-Continent Ecology Division
Eric S. Mead, Safety, Health and Environmental Management
Michael D. Kahl, Chair, Animal Care and Use Committee
Suzanne van Drunick, SSWR  National Program Director
Michael McDonald, SSWR Deputy National Program Director
Walt Nelson, SSWR Project Lead
Richard M. Greene, Chief, Ecosystem Dynamics and Effects Branch, Supervisory Research
   Biologist, Gulf Ecology Division
William Benson, Director, Gulf Ecology Division
Ben Scaggs, Director, Gulf of Mexico Program Office
Darrel Brown, Office of Water, OWOW
Michael J. Shapiro, Assistant Administrator, Office of Water
Harold Zenick,  Laboratory Director, National Health and Environmental Effects Research
   Laboratory
Jennifer Orme-Zavelata, Director, National Exposure Research Laboratory
S. T. Rao, Division Director, Atmospheric Modeling Division
Robin Dennis, Senior Scientist, Atmospheric Modeling Division

USEPA - GED Personnel
Janis Kurtz, Nutrients Team Leader
Michael Murrell, DO Task Coordinator
James Hagy
Diane Yates
John Lehrter, SSWR Task Lead
Jeanne Scott, GED Quality Assurance Officer
Matthew Harwell, Chief, Ecosystem Assessment Branch
Mace  Barren, Chief, Biological Effects and Population Response Branch
J.  Kevin Summers, Associate Director for Science

USEPA - MED - LLRFRB Personnel
David Miller, USEPA, ORD
Mark Rowe, USEPA ORD
Kenneth Rygwelski, USEPA, ORD
James Pauer, USEPA, ORD
Amy Anstead, ICF International
Phillip DePetro, ICF International
Timothy Feist, Trinity Engineering Associates,  Inc.
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Xiaomi Zhang, Trinity Engineering Associates, Inc.
Wilson Melendez, CSC
David Griesmer, CSC

US Navy - Naval Research Laboratory (Stennis Space Center)
Robert Arnone

 A.4 Project/Task Organization

This project is being conducted within the ORD Safe and Sustainable Water Research Program
(SSWR) and is described in the  SSWR Strategic Research Action Plan (USEPA, 2012). The
research is  being  conducted within Theme  1:   Sustainable Water  Resources;   Topic 2:
minimizing the environmental impacts of land use practices that lead  to the sustainability of
surface and subsurface water resources; Task 2.3D:  modeling the linkage between discharge
and nutrients from  the Mississippi River basin to the  Gulf of Mexico  hypoxia.  The  Project
Leader is Walt Nelson and the Task Leader is John Lehrter.

U.S. EPA Mid-Continent Ecology Division (MED) Role

This MED Research and Quality Assurance Plan (RQAPP) describes only those Gulf of Mexico
modeling activities that are conducted within the Large Lakes and Forecasting Research Branch
of MED.  Dr. Russell G.  Kreis,  Jr.  (MED  Branch Chief) is the principal  investigator  for this
project.  Dr. Kreis is responsible for developing and maintaining the official copy of this RQAPP.
MED  is responsible for the development, calibration,  confirmation, corroboration, sensitivity
analysis, and forecasting of a suite of Gulf of Mexico  Dissolved Oxygen  Models (GoMDOM)
including a  screening-level one-dimensional  (1-D)  GoMDOM  and three-dimensional  (3-D)
GoMDOM models on scales of 6 km x 6 km and 2  km x 2 km grid sizes.  See Figure 1 for
details on MED members of the modeling and support teams.

Project Collaborators

This large project requires products and expertise from  parties external  to MED (see Figure 2).
Partnerships have been established with the following:

The U.S. EPA Gulf  Ecology Division (GED) is a primary collaborator and partner in the  project.
John Lehrter of GED serves as the project Task Lead for the Safe and Sustainable Water
Resources (SSWR) program, Task 2.3D, Modeling the linkage  between  discharge and nutrients
from  the Mississippi River basin  to Gulf of Mexico hypoxia.  The work described in this MED
Gulf of Mexico Modeling RQAPP  is one of the projects under SSWR Task 2.3D. GED has been
providing analytical  chemistry data from the Gulf and serves as a critical expert advising MED
on ocean chemical, biological, and physical processes  related to modeling Gulf eutrophication
and hypoxia.  Results of the modeling work will  result in peer reviewed journal  articles
coauthored among MED and GED scientists and engineers.

U.S. Navy Naval  Research Laboratory (located at Stennis Space Center), through agreements
with the U.S. EPA  Gulf Ecology Division (GED), has been providing hydrodynamic model
transport fields from their Environmental Protection Agency Coastal Ocean Model (EPACOM)
developed for northern Gulf of Mexico water.
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 The U.S. EPA Office of Environmental Information, Environmental Modeling and Visualization
Laboratory (EMVL) has been providing support to the project in the areas of specialized sub-
modeling, visualization, and improvement of modeling run times.

U.S. EPA National Exposure Research Laboratory (NERL) Atmospheric Modeling Division has
been  providing  atmospheric fluxes  of nitrogen  compounds to the Gulf waters from their
Atmospheric/Air deposition model (CMAQ, CTM, MM5).

An  informal  collaboration has  been established  with the National Aeronautics and  Space
Administration (NASA) at the Goddard Space Flight Center, Greenbelt, MD. NASA has refined
algorithms for chlorophyll, total suspended solids, and particulate organic carbon based on Gulf
data from this project that are used in combination with MODIS AQUA  and SEAWIFS remote
imagery for comparisons to GoMDOM model output.

Project Clients

Gulf of  Mexico  hypoxia has been  a concern  and a priority focus for the USEPA for several
years.   The  study efforts described here  are anticipated to  support decision-making  by the
USEPA and  many other management groups identified on Figure 2 as our clients.  This figure
shows relationships and lines of communication among these  clients and project collaborators.
The Office of Water is the lead among the  USEPA clients with authorities regarding the Gulf of
Mexico  and  the Assistant Administrator for the Office of Water is Chair of the Interagency
Mississippi/Gulf  of Mexico Nutrient Task Force. The Office of Water is directly supported by the
USEPA Office of Wetlands, Oceans,  and  Watersheds and USEPA Gulf of Mexico  Program
Office, Region 4 and Region  6 as they have jurisdictional interests in the Gulf.

 A.5 Problem Definition/Background

Investigations of the Gulf of Mexico's inner shelf (Figure  3) in the coastal waters of Louisiana
and Texas have documented seasonal oxygen depletion in this zone during the past several
decades (Rabalais  et a/.,  1999,  2001,  2002).    Hypoxia,  defined  as dissolved  oxygen
concentrations of less than 2 mg/L,  has increased in intensity, size, and duration during the past
several decades, averaging an area of impact of approximately 15,000 km2. The areal extent of
the hypoxic zone (Figure 4 and Figure 5) in the past decade has been observed to be as great
as  22,000 km2  and  appears  to be the  largest  known  hypoxic  zone in the  waters  of the
conterminous U.S (Pew Oceans Commission,  2003;  U.S. Commission on Ocean Policy, 2004;
World Resources Institute, 2008).  The Mississippi-Atchafalaya River Basin appears to  be the
dominant source of macro- and micronutrients which affect the observed hypoxia (Goolsby et
a/.,  1999; Mitsch et a/., 1999) through the over-production of phytoplankton and the subsequent
decomposition of the  organic carbon that imposes  oxygen demand and  depletion on the water
column and underlying sediment interface.

The primary  environmental problem is the size, duration, frequency, and intensity of hypoxia in
the Gulf of Mexico. Hypoxic bottom waters of the Gulf of Mexico are a detriment to the overall
ecological  health of this system and have  had chronic and acute  effects on marine life. The
hypoxic zone inhibits the occurrence of marine  life, degrades the habitat  for many aquatic
organisms, and negatively impacts desired aquatic production.  The impact on immobile species
such as benthos and shellfish is initially a restriction of range and  loss of habitat followed by
mortality. Mobile species, such as fishes and shrimp, may be able to avoid the hypoxic zone,
but their movement and habitat become restricted.
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Marine systems are typically nitrogen-limited in contrast to freshwater systems which exhibit
phosphorus  limitation.  In each  case, the other nutrient, together with silica,  may  become
secondarily limiting when the primary nutrient is over-enriched.  Many coastal areas of the U.S.
have been enriched with nitrogen and are showing  signs of secondary phosphorus and silica
limitation,  as observed in  the case  of the Gulf of  Mexico.  The available historical  records
indicate that nitrogen loading has increased more dramatically than that of phosphorus (Figure 6
and Figure 7, respectively). The USEPA has adopted a dual  management approach  for both
nitrogen and phosphorus as it relates to the freshwater resources of the Mississippi Basin and
the Gulf of Mexico (USEPA, 2008b).  The relationships  among nitrogen, phosphorus,  carbon,
and solids loads and concentrations with algal  production, algal biomass, and oxygen  demand
are critical to the understanding of hypoxia in the Gulf of Mexico.

Previously applied water quality  models and approaches (Bierman et a/., 1994; Greene et a/.,
2009, Justic et a/., 2003; Scavia et a/., 2003,  2004; Hetland  and DiMarco, 2007; Morse and
Eldridge, 2007; Scavia and Donnelly, 2007) have yielded insights to Gulf hypoxia but questions
are being raised as to the suitability of their resolution and degree of uncertainty with respect to
confidence related to nutrient reduction forecasts and the final  target to be established.  These
models used relatively coarse segmentation schemes with limited spatial resolution; simplistic or
limited kinetics; very approximate hydrodynamics, including the flow direction of the Mississippi
River plume; and  simplistic sediment and dissolved  oxygen interactions.   Due to  these
limitations, consensus on  loading reduction targets have been  very difficult to reach when
confronted with a range of 30-65% for nitrogen and/or phosphorus based upon  modeling and
empirical approaches (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force, 2001,
2004, 2008a, 20008b; USEPA, 2008b; NRC, 2009).

The  body of investigative and mathematical  modeling  studies during  the past decade has
provided considerable insight into the Gulf hypoxia issue and its relationship to the Mississippi
River Basin; however,  a  number of recommendations for future work and improvements have
been outlined (Committee on Environment and Natural Resources, 2010; Mississippi River/Gulf
of Mexico Watershed  Nutrient Task Force 2001, 2004, 2008a,  20008b; Justic et al.,  2007;
USEPA, 2008b;  NRC, 2009).  Selected recommendations, gaps, and issues  to promote a
consensus modeling framework with supporting data  are presented below:

1) A sampling design is needed to support the development of an integrated, multi-media mass
balance modeling framework.

2) The sampling program should be  specifically-designed to reduce the uncertainty associated
with the empirical data and modeled nutrient-reduction forecasts.

3)  The sampling  program should be seasonally-driven  to create at least a full  2-year period
dataset and supplement the existing summer sampling program  by directed overlap.

4) The sampling program should be  statistically-based with  random transects and stations that
include  multiple  resource classes:  embayment/near-coastal,  inner shelf, outer shelf, and
bluewater.  The offshore  boundary should be sufficiently sampled to delineate  the boundary
condition.

5) Determine phytoplankton species and carbon flux seasonality.

6) Further define sediment diagenesis and sediment nutrient flux factors.
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7) Account for water column oxygen demand.

8)  Further quantify the relationships among loads, ambient concentrations, chlorophyll, and
dissolved oxygen, using data and models.

9)  Establish a  multimedia (air, water, sediment),  mathematical  modeling framework which
builds upon past efforts and includes a hydrodynamic model, atmospheric model, sediment
transport and fate model, and water quality-eutrophication model.

In order to address these factors, a high resolution, multi-media modeling suite is being applied
to address the atmospheric, hydrodynamic, water quality, and sediment interactions as well as
spatial resolution and improved kinetics. The modeling framework is also being supported by a
monitoring and laboratory program, specifically designed for the modeling. The  cornerstone of
the multimedia  construct  being applied  is the Gulf  of  Mexico  Dissolved Oxygen  Model
(GoMDOM), a version of  LM3-Eutro, which includes water quality chemical,  physical, and
biological interactions and kinetics with linkage capabilities to other modeling components.  The
modeling framework required primary productivity,  dissolved  oxygen (DO)  and other  kinetic
equations to realistically represent processes within the Gulf. A sediment-water component is
also necessary to account for this important process in the Gulf of Mexico.  Nutrient transport is
driven  by hydrodynamic output from the  U.S.  Navy Naval  Research  Laboratory's  (NRL)
EPACOM  model  (Ko et a/., 2003).   Atmospheric loads  of nitrogen  compounds are  being
provided by EPA's Community Multi-scale Air Quality Model (CMAQ).

The Gulf Hypoxia modeling  framework is being designed to  integrate monitoring, condition
assessment, diagnosis,  and  experimentation within a mathematical modeling  construct that
incorporates multimedia inputs,  environmental data, and  ecosystem dynamics  to establish a
forecasting capability.  Since a wealth of information is available to formulate the many transport
and kinetic processes and to estimate model parameters,  it is  believed that this deterministic
model will provide a better predictive estimate than using an empirically established relationship.
The goal of this collaborative effort is to develop a mathematical modeling framework that will
aid water resource managers in making scientifically defensible nutrient restoration decisions to
reduce the  hypoxia problem.  By  reducing the  size of the hypoxic zone,  it is suspected that
habitat and  food web assemblages  along the Louisiana-Texas  (LA/TX)  coast  will  benefit.
Specifically, the model will be applied to estimate dissolved oxygen concentrations  and hypoxia
area in  the northern Gulf of Mexico under several nutrient load reduction scenarios.  Other
major model outputs include the  duration of hypoxia, nutrient concentrations, and phytoplankton
concentrations.  This modeling effort will assist managers in helping them to understand options
available to achieve a goal  of  a  five-year running average hypoxia zone of  5,000  km2 as
specified by the 2001  and  2008 Gulf  of Mexico Action Plan  and  supporting documents
(Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2001, 2004, 2008a, 20008b;
USEPA, 2008b).

This Research and Quality Assurance Project Plan focuses on the following components of the
modeling project:

   •  Project management, objectives, and description;  quality objectives; special training
      needs; and documents and  records management.

   •  Data generation and acquisition
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   •   Model construct, coding, inputs, confirmation and  corroboration, sensitivity/uncertainty
       analysis, and application

   •   Assessment and oversight

   •   Data validation and usability

 A.6 Project/Task Description

The modeling framework will build upon earlier models that were developed and applied to the
Gulf of Mexico and is  most similar to the efforts of Bierman et a/. (1994).  The Gulf of Mexico
Eutrophication and Dissolved  Oxygen Model (GoMDOM) is based  on the LM3-Eutro water
quality model (Pauer et a/., 2006, 2008, 2011; Melendez et a/., 2009) that was developed for
and applied to Lake Michigan. GoMDOM, in  its present form, has salinity, two phytoplankton
state variables, one zooplankton  state variable, and several dissolved and particulate nutrient
state  variables.   The  model  uses standard eutrophication kinetics to  describe the many
biochemical reactions  such as: Monod kinetics to describe phytoplankton growth, first- order
nutrient mineralization  kinetics, and a temperature dependency function  for the  biochemical
reactions.  The  Jassby and  Platt equation  was used  to estimate  the limitation of  primary
production  by available light (Jassby and  Platt, 1976; Lehrter et a/.,  2009).  Light attenuation
was  calculated using  a site-specific relationship between light attenuation  and  chlorophyll,
particulate  carbon, and salinity.   It also  has simple  user-defined sediment-to-water  nutrient
fluxes. To  prepare LM3-Eutro for  its application to the Gulf of Mexico, the  model was modified
to use output from the  Navy hydrodynamics  model  (EPACOM)  and a dissolved  oxygen
subroutine  was included.   The model receives loadings primarily  from the Mississippi  and
Atchafalaya Rivers but also from minor tributaries and an atmospheric model (Community Multi-
scale  Air Quality (CMAQ)).   See Figure 8  and Figure 9 for  information on  the integrated
multimedia  model interactions.  This modeling framework will integrate multimedia  inputs (from
statistically-based monitoring programs) and ecosystem dynamics to establish a  model  that will
have  the forecasting   capability  for exploring  alternative futures  and/or remedial  options.
GoMDOM will  be calibrated and  corroborated using cruise data collected  from  2003  to 2007.
Uncertainty, sensitivity, and other statistical  analyses will  be performed  using the model  to
estimate  the accuracy of the model  predictions.  Finally, the model will be applied to  estimate
the dissolved oxygen  concentration and hypoxic area in  the northern Gulf of Mexico under
several nutrient reduction scenarios, including the allowable nitrogen load  that would limit the
hypoxic area to a maximum of 5,000 km2.

An overall project timeline is provided in Table 1. In 2007, advanced general project planning
took place.  In 2008, database development, which harmonized the various field measurements,
laboratory analyses, and  research results by media,  site, and  time,  began  along with model
development.   Considerable  model  calibration runs,  sensitivity runs,  and journal  article
preparation  took   place in FY2011  and  FY2012.   This timeline  is  based  upon  current
understanding  of the science affecting hypoxia on the coastal shelf and management objectives.
The schedule  should  be  considered preliminary and  may require adjustment if management
priorities  change because of future events, if scientific findings during the project indicate a need
to change the project scope, or if deliverables from project  partners are not received in a timely
manner.
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Model Development Strategy

The modeling framework is being developed following a two phase strategy (see Section B.1 for
more  details).   In  the  first phase,  the model  eutrophication,  DO, and  sediment-water
interactions are being developed and tested using a one dimensional (1-D) GoMDOM screening
model.  This screening-level model will provide for easier development  and testing  of water
quality kinetic equations.  It is being applied at selected stations in the Gulf of  Mexico where
data for  calibration purposes are  plentiful  and where  horizontal gradients of model  state
variables are minimal.  The model is run for a relatively short duration and consists of four water
layers and includes interaction with the sediment.  This  screening model  will not be  used for
model scenario forecasting,  although  it  should provide insight into  biological  and chemical
interactions on the coastal shelf.  Calibrated  model coefficients/parameters from the screening
model will be used to provide some initial estimates for similar coefficients/parameters in the
higher resolution GoMDOM models.

During the second phase, the model framework developed in the first phase will be applied to
an intermediate resolution model grid (approximately 6 km x 6 km and 26 sigma layers).  The
intermediate resolution model will be calibrated and corroborated to existing coastal shelf cruise
data.  The model  will then be applied as a diagnostic tool to assist in evaluating biochemical
interactions on the coastal shelf and applied  to selected  management scenarios. If needed, a
high resolution (2 km x 2  km) GoMDOM model will be implemented.

1-D GoMDOM Screening Model

The development of the screening model will include three tasks:  a review of previous modeling
efforts and available data, the application of the selected model to the Louisiana coastal shelf,
and the  modification of  the  model to  more appropriately represent  physical,  chemical,  and
biological processes on  the Louisiana  coastal shelf. The proposed schedule for  completing
these tasks is outlined in  Table 1.

Data/Model Review

This  task   included  reviewing  previous  modeling   efforts,  available  data,   and  other
recommendations and identified gaps to determine which modeling frameworks are  suitable for
use in the proposed modeling framework. An initial review of the GED cruise data and other
available data was conducted to assist in  determining the most suitable extent for the proposed
model grid and to determine what time periods and kinetic processes have sufficient data to
support modeling efforts.  Published studies were reviewed to help determine appropriate water
quality processes to include in the model framework. Previous water quality models, both from
the study area and those suitable to be applied to the study area, were reviewed for possible
use in the modeling framework.

Two mathematical models were considered as frameworks for developing the hypoxia model for
the Gulf.  CE-QUAL-ICM was developed by the US Army Corps of Engineers (Cerco and Cole,
1995) and applied to Chesapeake Bay (Cerco and Cole, 1994).  LM3-Eutro is another high
resolution framework that was developed and applied to Lake Michigan  (Pauer et a/., 2006,
2008, 2011;  Melendez et a/., 2009).  Both models had many positive attributes and very suitable
building  block  for the next the  Gulf model.  After  careful review and consideration,  the LM3
framework was selected  as a base for developing a new Gulf model. The results of the model
review found that LM3-Eutro has most of the features to address the hypoxia problem in the
northern  Gulf of Mexico.   Since this model was developed in-house and staff are familiar with
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the modeling framework, it can be relatively easily modified and applied to the Gulf study area.
Modifications include code  modification to utilize output from the Navy hydrodynamics  model
(EPACOM) and the addition of dissolved oxygen and sediment subroutines.

Hydrodynamic  models were  also reviewed.   The models  examined included  the  Princeton
Dynalysis  Ocean  Model (PDOM), Environmental  Fluid Dynamics  Code (EFDC),  Estuarine,
Coastal and Ocean Model (ECOM), Regional Ocean Model (ROMS), and the Navy's  EPACOM
model. These  were generally regional models with various spatial and depth operational limits.
The EPACOM  model is based on the Navy's Intra-Americas  Sea Ocean Nowcast/Forecast
System (IASNFS)  and was selected to provide the hydrodynamic transport fields. IASNFS is
based on the Navy Coastal Ocean Model (NCOM) (Martin, 2000;  Martin et a/., 2009).

The Community Multi-scale Air Quality Model (CMAQ) was selected to provide atmospheric
nitrogen compound loads to the water quality model. It is the premier national deposition model,
operated by EPA,  has nitrate deposition over the Gulf of Mexico, and is being  run with finer-
resolution deposition for the purposes of this study (Byun and Ching, 1999;  Byun and Schere,
2006; Dennis etal., 2007, 2008.

The initial model development task involved applying LM3-Eutro equations to the 1-D model grid
(four water layers with interactions with the sediment) to evaluate and test the application.
Simplification included limiting the model such that all phytoplankton were represented as a
single model state  variable along with fewer particulate nutrient and carbon state variables.
GoMDOM-1D used site-specific measurements and empirical relationships to determine nutrient
and oxygen demand sediment fluxes.  MATLAB was used in this model development.

After the initial  testing, the model will be modified as needed to appropriately simulate important
processes affecting hypoxia on the coastal shelf.  The model then will undergo  further testing
and evaluation to  ensure that physical, chemical, and biological processes are  being suitably
simulated. The 1-D GoMDOM will be used to allow for easier testing as the model framework is
being developed.   The majority of model confirmation/corroboration activities  (Section B.4)
related to kinetic processes have been completed.  The screening  model will not be formally
applied to management scenarios. The screening model will be compared to field data and may
provide insight into processes in the  study area that may need to be further evaluated. The
calibrated model coefficients from the 1-D GoMDOM will be used as initial estimates of  similar
coefficients for the 3-D GoMDOM models.  Any deficiencies identified in the model framework
will be  addressed,  and the model  re-confirmed,  before  application of  the  model  to the
intermediate resolution GoMDOM.

3-D GoMDOM  6 km x 6 km Intermediate Resolution Model

Finalize Intermediate Resolution Model Grid

The model grid for the 3-D GoMDOM 6 km x 6 km intermediate resolution model was  based
upon the  review  of  available  data.   The  grid  extends from the  shoreline  southward  to
approximately the  80-100 m contour and from east of the Mississippi  River Delta westward to
93° W longitude. This grid extent contains the area of hypoxia during most years and provides
sampling stations outside the  grid for  use as boundary conditions. The model is being applied
to an approximately 6 km x 6 km model grid, with hydrodynamic transport provided by the NRL's
EPACOM model output aggregated to this size (the original scale is  approximately  2 km x 2
km). EPACOM output provided for this project contains 26 vertical sigma layers which should
provide suitable resolution  of surface, pycnocline, and  hypoxia zone layers.   This grid size
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provides  a compromise between higher spatial resolution and faster model run times.  The
extent of the grid may be expanded to include additional areas of the coastal shelf if the initial
model results show it would be useful and if  hydrodynamic data are available to support an
expansion. The vertical resolution of the grid is identical to that of EPACOM.

Due to the hydrodynamic and biochemical processes in the study area that occur at relatively
small scales because of the shallowness of the shelf and the mixing and stratification of the
freshwater outflow from the Mississippi and Atchafalaya Rivers, a model with a resolution similar
to the  intermediate  resolution  GoMDOM  model  is  recommended for  properly  simulating
processes affecting hypoxia in this area.  In  the second phase of model development, the
knowledge gained from the first phase will be applied to the intermediate resolution GoMDOM
model.  The model will then be calibrated and corroborated against cruise and process data and
used for diagnostic evaluation of biochemical  processes and for management scenarios.  The
proposed schedule for completing these tasks  is included  in Table 1. This schedule should be
considered preliminary and may need to be  adjusted if findings from the screening model
suggest a change in project scope, if management priorities change, or if products from project
partners are not received in a timely manner.

Create Input and Linkage Files

This task included developing the model grid and geometry files, developing software to convert
the NRL hydrodynamic model output into a format that the water quality model can use as input,
creating input decks (model input files describing oxygen and nutrient initial conditions and
estimates for the model parameters), modifying the original source code to read input data and
to write simulation results to output using NetCDF, and running and testing the model. Software
developed in this phase, for example to generate mapping and linkage files, was designed so
the model framework can easily be applied to higher resolution model grids.

Calibration and Corroboration

The intermediate resolution model will  be tested to confirm that it is working properly (Section
B.4) and then calibrated and corroborated against GED cruise survey data following procedures
outlined in Section  B.5.   Data sets  for these procedures will be  selected from databases
completed by the time of the procedure.

An evaluation of model sensitivity and uncertainty (Section B.6) will be conducted concurrently
with the calibration and corroboration of the intermediate resolution model.

Model Diagnostic Testing and Scenarios

In this  task the calibrated and corroborated model  will  be applied  in  a diagnostic mode for
scientific evaluation of shelf processes and in scenarios  to assist in evaluating  management
options following procedures described in Section B.7.  Additional input files and hydrodynamic
inputs may need to be created depending upon the scenarios selected.

3-D GoMDOM 2 km x 2 km High Resolution Model

If the modeling results and analysis from the 3-D GoMDOM 6 km x 6 km model indicate that a
higher resolution model is warranted, and if time  permits, a 3-D 2 km x  2km high resolution
model will be utilized.  The modeling framework from the intermediate  resolution  GoMDOM
model is directly transferable to the 2 km x 2 km model. Also, once the intermediate resolution
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model  is  calibrated, those calibrations  (when  applicable)  can be  transferred  to  the high
resolution model.  This will be a time saver when calibrating the 2 km x 2 km model. However,
due to the large number of model cells in the high resolution model, the run times will be much
longer than that in the intermediate model. Consequently, an effort is being planned in FY2013
(see Table 1) to parallelize the code to significantly reduce the model run times. This model will
likely be run and tested on EPA's supercomputer in RTP, NC in FY2013.

 A.7 Quality Objectives and Criteria  for Measurement Data

Quality objectives and criteria will be established to ensure that the model output addresses the
management questions  with the accuracy required  by the  user.  This can be achieved  by
establishing statistical criteria to  determine,  during the model evaluation stage, if the overall
accuracy of the model is acceptable and if the model uncertainty is acceptable.
Level of accuracy and precision of model output
Before a model is used for remedial guidance and/or regulatory purposes, agreement between
the expectations of the managers who will be using the model and the model  developers is
needed.   Managers  need to be  generally well  versed in the science of modeling  natural
systems.  Modelers have the responsibility of not only attempting to make the models reliable
but also  to state unequivocally their assumptions and uncertainties.  This is usually done by
providing the most probable answer(s) along with uncertainty brackets which provide a range
that is very likely to contain the actual answer.  The decision-maker must determine whether to
use the model with the uncertainties and caveats provided or to provide additional resources to
refine the results.  If refinement is needed, the modeler can advise management on what needs
improvement because of their knowledge gained in determining model sensitivity to various
model-controlling forcing functions or processes.

Modeling quality objectives continue  to be discussed regarding the Gulf of Mexico  and will
depend upon the certainty required by managers and the importance of the modeling tool in
developing nutrient loading targets to reduce the extent of hypoxia.  With respect to these
concerns, a preliminary data quality objective (DQO) is for the model to simulate the average
water quality within plus or minus two standard errors of the mean  of the field measurements,
meaning there is approximately 95%  confidence that the actual  model-predicted result falls
within this range.  It is likely that the model fit to data will be much better than this criteria for
many of the model-predicted state variables.  The data means and standard  errors will be
computed  using  appropriate spatial  and temporal  statistical averaging  and  interpolation
techniques.

Obviously, the range of plus or minus two standard errors of the mean of the measurements is
(in part) a function of measurement (including both sampling and instrument) precision. Most of
the field data used in model calibration and confirmation will originate from the U.S. EPA Gulf
Ecology Division.  The quality objectives and criteria for these data are described in the Gulf of
Mexico  Hypoxia Quality Assurance Project Plan  (Greene, 2007).   In  this  document,  most
parameters have an instrument accuracy target of 10%  and an instrument precision target of
30% Relative Standard Deviation, also known as the Coefficient of Variation.

Prediction bias will be minimized by calibration, the process of parameter optimization seeking
to minimize residuals (the difference between model calculated and measured concentrations),
without  violating constraints imposed by scientific  observations  and  principles.   Modelers
commonly plot field observations vs. model output for a  given model state variable (Pineiro et
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a/., 2008).  This method provides both qualitative and quantitative feedback to the modeler on
how well  the  model compares  to field observations.   If the model  predictions match  field
observations, then it is expected that the residuals (difference between the plotted points and
the 1:1 line) fall randomly about the 1:1 line and are relatively close to that line.  Model biases
can be noted using this method when a majority of points lie either above or below the 1:1  line.
If a majority of the points fall either above or below the 1:1 line, then a serious model bias exists
and will be explored further to determine the cause.

An R-squared  of the correlation described in the previous paragraph provides information on the
"goodness of fit" of the model to  observations.  In a calibration exercise, the modeler will try to
maximize  the R-squared.  However, no target R-squared can be established because this can
vary from  state variable to state variable.  For example, modeling a conservative substance like
salinity may yield a maximum achievable R-squared of 0.8; however, for a much more complex
state variable involved in a multitude of kinetic reactions  such as nitrogen, an R-squared of 0.5
may be the best that can be achieved.

The model will be considered calibrated when the results for important model state variables fall
within the 95% confidence intervals of the majority of the data cruise means and the results
have a highest  achievable R-squared when correlating model output to field observations,
stratified  appropriately in  time and  space.   In addition, model simulations will  attempt  to
reproduce the statistical distribution properties of the data.  This will be evaluated by comparing
cumulative frequency distribution plots of data  to frequency distribution plots from comparable
model predictions.

Once calibrated to field data, the  model will be valid within the error constraints specified for the
calibration period.  However, for forecast predictions, it is  not possible to know the uncertainty of
predicted  forcing functions and  boundary conditions.   Therefore, the model will  be run for
various forecast  scenarios with inputs  bracketed  in  terms of extreme  expectations  and
probability  distributions,  and  the results  will be provided in terms  of prediction  means  and
exceedance limits.

Criteria for using secondary data (literature values, etc.)

Data generated specifically for this project will be used for model development and calibration;
however,  where no  project data are available, data from the literature  and other modeling
studies will be used.  The majority of data to be used  as  model inputs  originate from the Gulf of
Mexico Hypoxia Study Project and samples are being  collected and analyzed following the  U.S.
EPA Gulf Ecology Division's  "Gulf of Mexico  Hypoxia  Monitoring Survey Quality Assurance
Project Plan"  (Greene, 2003; Greene, 2007).   The monitoring QA plan  describes the QA
program and process, organizational structure, data  quality  objectives, implementation of the
QA program, and  information  management guidelines for the data collection activities  of the
study.  All GED's analytical data for the model's target analytes and most supporting data will
have been verified through their QA  program's  process and will have met the performance
criteria established before release to modelers. Data will  undergo an additional screening by
project modelers to ensure suitability for modeling purposes

Data generated  through other projects or studies may  be obtained  from either published  or
unpublished sources. The published data (including  those from gray literature) will have had
some degree of QA review, although there is  a wide range  of review quality among possible
sources.    Unpublished  databases  may  be obtained directly from  authors  or  from on-line
databases.
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When possible, all data used by the modelers will be checked for bias, comparability, outliers,
normality, completeness, precision, accuracy, validity of station names and sample identification
codes, and  units errors.   Modelers will also review  any documentation  or  data qualifiers
accompanying data sets. As questions arise, we will contact the data generator if possible.

Negative consequences of making inappropriate decisions due to poor model prediction
ability

The  chances of making inappropriate decisions due to poor  model prediction ability will  be
minimized through the quantification and evaluation of the accuracy and reliability of model
predictions  (Sections A.7, B.5,  and B.6) and through the reconciliation of model prediction
reliability with user requirements (Section D.3). In addition, the Gulf of Mexico hypoxia model
proposed for this study is  only one of several tools that will be  used by managers when
determining  management  and  regulatory  options  for  the  Mississippi  River/Atchafalaya
River/Gulf of  Mexico system.   Other  tools include  previous modeling  studies, on-going
monitoring efforts, and summary reports by scientific  panels  such as the Science Advisory
Board  Hypoxia Panel.   As one of many tools available, the proposed model will provide
additional weight of evidence to proposed nutrient management options and provide additional
insight into ecological processes affecting hypoxia,  but it will not be the sole determining factor
in management decision-making. Model results will include estimates of reliability provided by
modelers and reviewers that will guide managers in how much weight to place on model results.

 A.8 Special Training Needs/Certification/Expertise

Two primary categories of specialized training and certification are envisioned.  Typically  an
environmental  engineering degree or environmental science  degree with training in  systems
science is suggested for mathematical modelers.  With the Agency's emphasis on  integrated,
multimedia,  modeling, it is  valuable to  have  a broad  background that includes the aquatic
sciences (chemistry,  biology,  and physical processes).   A degree  in computer sciences  is
recommended for model programmers and database specialists. In both cases, degrees may
be in other primary disciplines that enable each to conduct the  respective job skill. In addition,
strong backgrounds in mathematical sciences and statistical analyses are typically necessary.

Additional specialized (such as geographical information systems) training  will be provided if
needed on an individual basis and will be documented by the project leader.  The project leader
will be  responsible for assuring that the modeling staff have the training necessary to complete
the project.

All modeling staff (both federal and contracting staff) will be required to have had training in
NHEERL/MED's Quality Assurance program. This QA training course covers the following
topics for new hires: QA Orientation, Laboratory Recordkeeping, QA Planning Documents,
Operating Procedures and Technical Systems Audits. Every three years, all scientific staff will
be required to attend a QA Refresher Course.

 A.9 Documents and Records

A PDF copy of the Gulf of Mexico Research and Quality Assurance Project Plan (this  document)
with  all  signature approvals will  be made  available  via  the Internet.   The notification of
accessibility of the approved plan will  be sent to those individuals and organizations listed in
Section A.3 of this plan.  Any modifications resulting from an annual review of the  plan will also
be posted on the designated web site as addendums to the plan.
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A Study File (project records at the completion of a project) will be prepared by the Principal
Investigator at  the  termination of the project.   The Study  File will contain  all necessary
information to substantiate any  project findings  and will include both paper and  electronic
records. Any relevant electronic model records not physically contained in the Study File (such
as very large model files) will be stored within a model  archive located on a local server.  An
index to the materials in the Study File will be included. The contents of the Study File  may
change as needed and directed by the Principal Investigator.  The Study File contents  for the
Gulf of Mexico hypoxia modeling project will contain:

    •   Research and Quality Assurance Project Plan

    •   Applicable Operating Procedures related to modeling

    •   Study-related correspondence including  Gulf of Mexico modeling  meeting minutes
       between MED-Grosse lie and Gulf Ecology Division; MED-Grosse lie and the RTP, NC
       Environmental  Modeling and Visualization Laboratory.

    •   Model archive describing where input and output files are located, source code, and any
       other files related to running the Gulf models

    •   Electronic media with  the field data used in the project will be placed in the study file

    •   Any peer-reviewed journal articles related to the project

Principal   model  documentation  will  be  provided  within  the  electronic  model  archive.
Documentation  of the models will include  a description of the model construct (including the
governing  equations), model calibration  and validation runs,  model  input and  output,  and
"readme" files. Sensitivity and uncertainty analysis results will be archived along with the model
computer code (both source and executable files). Internal documentation is also maintained in
the header comments of each program subroutine.  A summary of field, literature, and external
data sources used in the model input, calibration, and validation process will be documented.

A complete description of the model equations, underlying assumptions, and numerical methods
can be found in several user manuals including  CE-QUAL-ICM (Cerco and Cole, 1995), the
LM3-Eutro model (Paueref a/., 2006, 2008, 2011), and the LM3 model manual (Melendez et a/.,
2009). All functional changes made to the model program will be documented  along with the
new code within the electronic Revision Control System (RCS) that maintains all versions of
modeling code used at LLRS and serves as the model code archive.

Various model products will  be prepared  throughout the project. These will include  interim
reports, and at the request of management, oral  presentations will also be given periodically.
Presentations at scientific meetings  will be encouraged  on  any aspect of Gulf modeling.  The
Gulf of Mexico modeling project would likely be classified as a QA Category II,  or research of
high programmatic relevance which, in conjunction with other ongoing or planned studies, is
expected  to  provide  complementary support of Agency  rule-making, regulatory, or policy
decisions (USEPA, 2005).  Because of this designation, significant findings from the study must
be published in peer-reviewed scientific/engineering journals. If publication does not occur,  then
a formal review of the project and results will  be  required through a formal peer panel  review
process.
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As  a QA Category II  project, the retention and  disposition of project records must  be in
accordance with the Agency's National Records Management  Program led by the  Office of
Environmental Information.  Records retention schedule under  Function Code 501 (Function
Number 316-258) would likely be applicable to the Gulf of Mexico project.  These records are
first stored at the office that generated them for three years after the files have been closed;
then transferred to the National Archives and Records Administration (NARA); Federal Records
Center (FRC) for 20 years; and then a final transfer to the National Archives for permanent
archive.  The MED Technical Information Officer will manage the transfer of the records  to the
appropriate archival entity. Details can be found  at EPA Records Schedules by Function Code
established in 2/20/2007 at http://www.epa.gov/records/policy/schedule/function.htm.
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         GROUP B:  DATA GENERATION AND ACQUISITION



 B.1 Model Formulation

Study Area

The study area is the Northern Gulf of Mexico and the Louisiana Continental Shelf.  It extends
from the Mississippi Delta west to the Louisiana-Texas border, and from the shoreline seaward
to the 60-100 m bathymetric contours (approximately 26°N to 30°N by 88°W to 94°W - see
Figure 3). The average depth of the hypoxic zone is approximately 20 meters.  The Mississippi
and Atchafalaya Rivers account for almost all of the freshwater entering this part of the Gulf.
This area is strongly stratified over the April to October period, largely due to salinity gradients.
Approximately 50% of the autochthonous material produced in this area settles to the sediment,
resulting in carbon and nutrient rich sediments.  A description of the system and causes of the
hypoxia in the northern Gulf of Mexico was described in Section A.5.  Details can  be found
elsewhere (Rabalais and Turner, 2001; Rabalais et a/., 2002; Dagg et a/., 2007)

Modeling Framework

The model design for the  Gulf of Mexico is based  on the linked sub-model approach as was
used in the Lake Michigan Mass Balance Project (Pauer et a/.,  2006).  It consists of a water
quality  model that  includes eutrophication and DO kinetics that is driven by output from a
hydrodynamic model and a coupled sediment model. At this time, however, the  sediment model
has not yet  been incorporated into the model. The water quality model receives tributary
loading inputs directly and  atmospheric nitrogen compound loads from an atmospheric fate and
transport   model    (CMAQ)    developed   and    run    by   our    collaborator,    U.S.
EPA/ORD/NERL/Atmospheric Modeling Division.  A schematic representation of the overall
mass balance design is shown in Figure  8.

Hydrodynamic Model

Hydrodynamic models developed and maintained by the Naval Research Laboratory (NRL) in
Stennis, Mississippi are being used to describe the hydrodynamics of the Gulf of Mexico for the
modeling framework.  The screening model and the high resolution model use output from the
NRL hydrodynamic model  EPACOM (Northern Gulf of Mexico Coastal Ocean  Model for EPA)
(http://www7320.nrlssc.navy.mil/IASNFS_WWW/EPANFS_WWW/).   This  model  covers the
coastal areas of Louisiana, Texas, Mississippi, Alabama, and part of Florida.  For purposes of
the hypoxia modeling, only output from  the study area is being used. The model uses a high
resolution grid that has an approximate size of 2 km  x 2 km and 34-40 vertical layers, consisting
of 26 proportional-depth sigma layers on the shelf and 14 fixed-depth layers beneath the sigma
layers in deeper Gulf waters.   The intermediate resolution  model  utilizes EPACOM vertical
mixing  coefficients  that has been  aggregated  into a 6 km x 6 km horizontal grid.  Water
temperature  and salinity are taken directly from measurements.  The high  resolution 2 km x 2
km version of the model (if needed) will use the original 2 km x 2 km output from EPACOM.

The  main goal of the hydrodynamic model will be to generate three-dimensional fields  of
currents and temperature in the Gulf. Currents are very important for the transport simulation of
state variables, while water temperature is  a critical forcing function of algal  growth.  Other
parameters  that the  hydrodynamics  model  provides  to  the water  quality  model  are


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hydrodynamic vertical diffusion coefficients and salinity and sea surface heights.  Cell volumes
are calculated  using the sea surface  heights, undisturbed water depths,  and the sigma layer
percentages.  Horizontal diffusion coefficients are not archived by the EPACOM model but have
been calculated from EPACOM model  output.  Diffusion coefficients are needed by the transport
part of the simulation.   Diffusive and advective transport are non-trivial components of the
overall movement of particles in the water column.  Grid  cell volumes are also needed in the
transport calculation  of the water quality  model  and  in the calculation of state-variable
concentrations.

The aforementioned parameters will be calculated  by the hydrodynamic  model and averaged
over an appropriate time span for the selected periods for model calibration, corroboration, and
scenarios.  The averaging interval used for the intermediate resolution model is one-hour for
flows and sea surface elevations and three-hour for temperature and vertical mixing coefficients.

Water Quality Model

The transport algorithm is  based on the CE-QUAL-ICM and LM3-Eutro modeling frameworks
(Cerco and Cole, 1994; Pauer et al., 2006, 2008, 2011) that were applied in Chesapeake Bay,
Lake  Michigan, and  other  systems.   This  algorithm describes  the movement of nutrients,
phytoplankton and other constituents in the system.

The one-dimensional Gulf of Mexico hypoxia modeling framework, GoMDOM-1D, is largely
based on the three-dimensional Lake Michigan Eutrophication Model "LM3-Eutro" (Pauer et al.
2006,  2008, 2011; Melendez et al., 2009). However, a number of simplifications were made
which include a one-dimensional single vertical water column, 6km x 6km scheme, a single
phytoplankton state variable, and fewer nutrient state variables.  However, the model uses a
revised light limitation formulation and simulates dissolved oxygen in the system.  GoMDOM-1D
uses site-specific measurements and empirical relationships to determine nutrient and oxygen
demand sediment fluxes.

The one-dimensional  approach is based on the assumption that horizontal advective flows and
diffusion across the boundaries of the column are negligible  during the  time scale of model
simulation (-100 hours), and thus the model is  defined as an isolated, layered batch reactor.
This isolation allows for the  parameterization of kinetic and vertical  processes in the Gulf of
Mexico exclusive of  the effects of horizontal transport.   Defining process  kinetics  through
GoMDOM-1D assists in the calibration of the three-dimensional model, GoMDOM-3D model,
which is being developed concurrently  with this work.

For the 3-D intermediate resolution GoMDOM model, the study area has a  grid  structure of 6 km
x 6 km horizontal segments and 26 vertical sigma levels. For the intermediate resolution model,
thickness, and thus volumes, of individual cells will vary significantly from relatively small cells in
the nearshore regions to much larger cells in the deeper areas of the Gulf.  The hydrodynamic
transport and eutrophication kinetic equations will be incorporated into this high resolution grid.
The transport is based on the integrated compartment method or box model methodology which
is a loose extension of the WASP model (Ambrose et al., 1993). The box model concept will be
retained in  order to allow the coupling, via map files, of the eutrophication/DO model with
hydrodynamic models of different dimensions and degrees of complexity.  The transport will be
performed as a one-dimensional exchange between two adjacent cells through  an individual cell
face, irrespective of the dimensionality of the model.  The model will handle horizontal  and
vertical transport during separate operations.  The constituent transport equation can be written
as follows:
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                    - — (Dx —) - — (Dz —) = 0                               (B.1)
 dt    dx     dz    dx  x dx    dz   z dz


F  =   constituent concentration (mass volume"1)
U  =   horizontal cell face velocities (length time"1)
W =   vertical cell face velocities(length time"1)
Dx =   horizontal cell face hydrodynamic diffusion coefficient (area time"1)
Dz =   vertical cell face hydrodynamic diffusion coefficient (area time"1)
x  =   horizontal dimension
z  =   vertical dimension

This transport equation is  solved using the third-order accurate Non-Uniform Grid ULTIMATE
QUICKEST algorithm (Leonard, 1991;  Chapman et a/., 1997) in the horizontal and second-order
implicit Crank-Nicholson  scheme in the vertical.  A detailed discussion can be found elsewhere
(Melendez et a/., 2009).

Like the 1-D GoMDOM model, the kinetic equations for the 3-D GoMDOM  are  based on the
LM3-Eutro modeling framework (Pauer et a/., 2006, 2008, 2011;  Melendez et a/., 2009).   A
schematic diagram of the  state variables and transformation reactions is shown in Figure 8.
General equations for phytoplankton  (chlorophyll-a) and dissolved oxygen are shown below.
Detailed equations  of the other variables  and transformation  equations  can be found in
Appendix 1 (equations which were not described in LM3-Eutro) and  elsewhere  (Pauer et a/.,
2006, 2008, 2011; Melendez etal., 2009).

General phytoplankton equation

The kinetic change in phytoplankton concentration can be written as:


V dP - v k
  ~dt  =    9~  d        9Z                                                        (B'2)


where

V  =   volume
P  =   phyt
t   =   time
kg  =   phytoplankton growth rate constant (time"1)
kd  =   phytoplankton mortality/respiration rate constant (time"1)
kgz =   predation rate (time"1)
Z  =   zooplankton concentration (mass volume"1)

The growth rate can be written as:

kg=kgmaxf Nf Tf I                                                              (B.3)


where

       =   optimum growth rate constant (time"1)
P  =   phytoplankton concentration (mass volume"1)
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f(N)   =   nutrient growth dependency
f(l)    =   light growth dependency
f(T)   =   temperature growth dependency

General DO equations

The general dissolved oxygen  equation,  often expressed as the Enhanced Streeter-Phelps
equation, can be expressed as follows:


V—-—-= Reaeration - Carbon oxidation - Nitrification
    dt                                                                         (B.4)
           - Respiration + Photosynthesis - SOD


where

V               =   volume
[DO]            =   dissolved oxygen concentration (mass volume"1)
t                =   time
Reaeration      =   oxygen exchange across the air-water interface (mass oxygen time"1)
Carbon oxidation  =   oxygen consumed due to organic carbon oxidation (mass oxygen time"1)
Nitrification      =   oxygen consumed due to ammonia oxidation (mass oxygen time"1)
Respiration      =   oxygen consumed due to algal respiration (mass oxygen time"1)
Photosynthesis   =   oxygen produced due to algal photosynthesis (mass oxygen time"1)
SOD            =   oxygen consumed due to sediment processes (mass oxygen time"1)

Detailed oxygen equations can be found in Appendix 1.

Sediment Diaqenesis  Model

It is well known that the sediment is a major oxygen sink and an important contributor to the
problem of summer hypoxia in the Gulf of Mexico. Algae and detrital material settle to the
sediment bed and subsequent diagenesis of organic material occurs.  This diagenesis process
results in nutrient and  reduced carbon (oxygen demand) fluxes from the sediment to the water
column.  A good understanding of sediment processes and formulation of a predictive sediment
diagenesis model is necessary to describe and predict nutrient fluxes and oxygen consuming
processes. Initially, nutrient and oxygen fluxes between the water column and sediment will be
described in the model using user-defined fluxes or as empirically-derived relationships based
on recent studies performed in the  northern Gulf of Mexico (Murrell and Lehrter, 2011; Lehrter et
al, 2012).  The empirical equation below (Murrell and Lehrter, 2011) represents the sediment
oxygen demand (consumption).  It calculates the amount of dissolved oxygen per unit time per
unit area (kg O2/m2/s)  that gets consumed or removed from the bottom layer of the water
column by the sediments.

                    SOD= 0.094xCDOx 106/32 -1.35  x3.7xl(T10              (B.4)
where
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SOD = sediment oxygen demand (kg O2/m2/s)
CDO = dissolved oxygen concentration of water column bottom layer (kg/m3)
A  sediment diagenesis model  will  be developed when field  data and process studies  are
sufficient to support it.  Figure  9 illustrates the sediment diagenesis model.  The diagenesis
model will be based on the sediment model developed for and  applied to Chesapeake Bay (Di
Toro and Fitzpatrick, 1993) and will be incorporated into the water quality model with the detrital
particles settling out of the water column onto the sediments. The sediments are represented
as two layers. The upper layer is in contact with the water and may be oxic or anoxic depending
on dissolved  oxygen concentration  in the  overlaying water. The  lower layer is  permanently
anoxic.  The depth of the  upper layer is variable while the depth of the lower layer is fixed.  A
general mass balance equation for the two layers can be written as follows:
         = J + KL12  c(2) - c(1)  - co2c(1)

                                                                                (B.5)
H2       = co2c(1) + KL12  c(1) - c(2)  - co2c(2)
where

H!     =  depth of surface layer (length)
H2     =  depth of bottom layer (length)
c(1)    =  concentration in surface layer (mass volume"1)
c(2)    =  concentration in bottom layer (mass volume"1)
t       =  time
J      =  flux (mass area"1 time"1)
KL12    =  mass transfer coefficient between layers (length time"1)
oo2     =  sedimentation velocity (length time"1)

Detailed equations can be found in Appendix 1.  Because the sediment model is  a  coupled
model  (incorporated into the water quality model), it will  be updated at the same time as the
water quality model. Although it can be difficult to obtain accurate values for sediment model
parameters, the full sediment diagenesis model has a major advantage over using user-defined
sediment fluxes  for predictive capability.

Atmospheric Model

The  atmospheric  component of nitrogen load to  the  surface water  of  the Gulf is generally
estimated at 2% of the total nitrogen load to the Gulf. CMAQ (Community  Multi-scale Air Quality
model),  or  Models3/CMAQ  (http://www.epa.gov/asmdnerl/CMAQ/cmaq  model.html),   will
provide MED with atmospheric fluxes to the surface  water segments  of GoMDOM  of  both
reduced and oxidized nitrogen compounds.  CMAQ and GoMDOM are  run independently of
each other.  Fluxes will  be provided for both wet and dry (gaseous and particulate) deposition.
The CMAQ grid  will be overlaid onto the GoMDOM  grid.  GIS tools will be used by the MED staff
to estimate a surface area-weighted flux to all surface  water cells of GoMDOM  to yield a load
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(mass/time). The aggregation of CMAQ fluxes into loads for GoMDOM is not expected to be a
major effort for the MED staff. CMAQ  does not make estimates of atmospheric phosphorus
loads.

CMAQ is a grid-based chemical transport model (fixed, regular grid) that can be nested from a
continental/sub-continental  scale (at 36-km and 32-km grids) down to finer scales over multi-
state geographic regions.  Nitrogen  deposition will be  input from  a 12 x 12 km grid for the
purposes of this project.  It is driven by a mesoscale meteorological model (a weather model),
currently  MM5  from Penn  State/NCAR  (National  Center  for Atmospheric Research).
Precipitation volumes are adjusted by the Parameter-elevation  Regressions on Independent
Slopes Model (PRISM).  CMAQ also incorporates the effects of  lightning on the generation of
nitrates.  CMAQ is  not a calibrated model, per se, but tries to work as much as possible from
basic scientific theories.  It outputs on an hourly  time-step  and  requires significant computer
resources to run.

CMAQ computes the gas- and particle-phase concentrations of the  inorganic  N nutrients of
ammonia (reduced  nitrogen) and nitric acid (oxidized nitrogen).  In the eastern U.S., a majority
of oxidized-N  air  concentration  is  gaseous  nitric  acid, and  a  majority of reduced-N  air
concentration is particulate ammonium.  An aqueous chemistry  and cloud module is used to
derive  rainwater  nutrient and  pollutant concentrations for the computation of wet deposition,
given the precipitation predictions from PRISM. Dry deposition algorithms are parameterized for
different land use categories for the gases and particles to determine the dry deposition rates by
grid cell.

 B.2 Model Coding

The  water quality  model is  written using FORTRAN  90/95 programming language.  This
programming   language  is  suitable for  models  that require  very  intensive numerical
computations, such as the GoMDOM models. FORTRAN 90/95 has all  the features that are
important  to scientific programming and most of the features of an object oriented language.
The language is designed to generate executable codes that are highly optimized and, thus, run
extremely fast.   FORTRAN  90/95 also supports parallel programming, making it an  ideal
language for implementation of the water quality model on parallel computers if the hardware is
available.

The  3-D  GoMDOM  models  are  being run on  high-end  Linux-based  computers.   These
computers are relatively  inexpensive and easy to maintain and  update.  The Linux operating
system offers the advantages of  low cost, high stability, high performance, easy networking,
multitasking, compatibility with UNIX software packages, and high  security.

The model source  code  will  use  external libraries which will be needed to handle input and
output tasks in addition to what FORTRAN 90/95 provides. The application will be reading large
sets of input data and at the same time writing a large amount of model calculations to output;
thus the use of a library to  store and document the data  in binary format will be required.  The
library chosen to handle  those tasks is known as the Network Common Data Form (NetCDF).
This library, in addition to using a binary format, allows the modeler to create, access, and share
array-oriented data in a form that  is self-describing and portable.  Self-describing  means that a
dataset includes  information defining the data it contains.  Portable means that the data in a
dataset is represented in a form  that can be accessed by computers with different ways of
storing integers, characters, and floating-point numbers.  NetCDF will be implemented within the
water quality model by using a Fortran interface. This interface consists of a number of routine
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calls that will be made within the program to the NetCDF library.  The library will be linked to the
water quality model at compilation time.

Libraries will also be needed to handle  reporting messages  that convey some  kind  of
information, warnings, and/or errors that might occur during the execution of the source code.
Reporting messages  are important when performing  simulations because they can  let  the
modeler know if the model is running smoothly or if bugs are present in the source code.

The  systematic development of a model  requires the  use  of a source code  revision tracking
system.  Thus the model will be archived and source code changes will  be tracked using  the
source-control system known as Revision Control  System (RCS).  RCS offers the ability to
record source code file revisions, retrieve previous file  revisions, control new revision creation,
record description of changes made  to a  revision,  control  who can make  source code
modifications, and specify user-access to source code files.  RCS uses a separate archive file to
hold  all the revisions of a given source file. Each  revision of a file that is put into an archive file
is  assigned  a revision number.  The archive files will be stored and maintained under  the
computer account of the person in charge of managing RCS.

Input and output files related to the model will be archived  under a designated directory in  the
Linux system.  Depending on the size of a given file, it  will be stored in binary or ASCII format.
Large input and  output files are better stored using a binary format to save disk space which
calls for the use of a library such as CDF or NetCDF.

 B.3 Model Inputs

Parameter estimation

The  Eutrophication/DO  and sediment diagenesis  sub-models consist  of  many biochemical
transformation reactions which  require estimates for a large number of model parameters.   For
this study, a number of coefficients will be obtained from Gulf of Mexico in-situ and laboratory
measurements,  including  phytoplankton  growth parameters based  on primary  production
measurements and SOD coefficients based on laboratory studies.  However, the majority of the
model parameters (similar to  most other eutrophication/DO studies) will be based on values
from similar modeling studies and measurements reported in the literature.  Several  of these
model coefficients will be adjusted during  the model calibration process (see Model Calibration
and Corroboration section) in order to obtain a final value.  Adjusting of model coefficients  will
be done within a reasonable range of reported literature values.  Model parameter uncertainty is
discussed in Section B.6.

Initial conditions

Initial conditions for a number of variables (Table 2) in the water column and sediments  are
required for the model.  These include values for dissolved oxygen, dissolved and particulate
nutrient species, phytoplankton and zooplankton  densities, organic carbon,  and salinity in  the
water column. Initial values for the sediment diagenesis variables include organic carbon and
nutrients.  Data from the first and perhaps second field surveys of a specific year of interest will
provide the majority of the values for initial conditions for the model. However,  it is possible that
values for some of the initial conditions cannot be calculated (directly or indirectly) from the field
surveys. In these cases,  values will be  determined using peer-reviewed literature, technical
reports, similar modeling studies, or unpublished databases. Data from peer-reviewed journals
have been subjected to a certain amount of review, but these data will  be examined by  the
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modelers for QA.  Unpublished data from reliable sources will be examined thoroughly and
analyzed with respect to QA. Section A.7 details QA procedures for data obtained from outside
USEPA.

Loadings

Nutrient loadings from atmospheric and tributary sources are essential to the calibration of the
Gulf of Mexico model.  Current loading  estimates are also important when performing  load
reduction scenarios to meet management objectives.  Atmospheric nutrient loadings are being
provided  by the atmospheric  model (CMAQ) as was described in Section B.1.  The USGS
calculates monthly nutrient loads in the Mississippi River from water quality data collected  near
St.  Francisville, LA and from flow data  from Tarbert  Landing,  MS.  Monthly  loads for the
Atchafalaya River are calculated based upon water quality data from Melville,  LA  and flow data
from Simmesport, LA.  Loads for nitrite plus nitrate and silica for the mainstem Mississippi River
extend as far  back as October 1967.  From October 1981 to September 2007 monthly  load
values for both rivers are available for nitrite plus nitrate, total Kjeldahl nitrogen (organic nitrogen
plus ammonia), ammonia, total  phosphorus, ortho phosphorus,  and  silica (Aulenbach et a/.,
2007; recent loads at: http://toxics.usgs.gov/hypoxia/mississippi/flux_ests/delivery/index.html).
In addition  to monthly loads, nitrite plus nitrate  and ortho phosphorus daily concentration and
flux values are available for the Mississippi River at  Baton Rouge, LA and nitrite plus nitrate
daily concentration and flux values are  available  for the Atchafalaya River at Morgan City
(USGS, 2006).   Water quality concentrations and  flows from the USGS (USGS, 2006;
http://waterdata.usgs.gov/nwis/) are used  to calculate  loads for parameters not provided by the
USGS and  for smaller tributaries that are not included in the USGS loading estimates.

Other forcing function estimations

Water temperature and advective  and vertical dispersive flows are obtained from the output
from the  hydrodynamics models as  described in Section B.1.   The NRL also provided solar
radiation  and wind velocity data that were used in EPACOM.   Horizontal  dispersive flows are
calculated in-house using data from  EPACOM and algorithms provided by EMVL. Flows  from
the  Mississippi and Atchafalaya  Rivers  will be obtained  from  the  US  Geological Survey
upstream stations (USGS,  2006).  The study area modeled has a large boundary with Gulf of
Mexico open waters and is strongly affected by movement across this boundary.  Boundary
concentrations are estimated from a number of cruise  measurements made at stations adjacent
to the study area, while exchanges  are determined from the hydrodynamics model.

Field data

Accurate and reliable  field data  (Table 2) are essential for  model development,  estimation of
model coefficients, and model confirmation.  In support of this project and the modeling study,
sampling was undertaken by the USEPA Gulf Ecology Division.  Multiple sampling cruises were
conducted  during several  years from  2003 to  2007.  The Gulf was sampled  for dissolved
inorganic nitrite plus nitrate, dissolved ammonium, particulate nitrogen, total dissolved nitrogen,
total nitrogen, physical parameters, biological parameters, and other chemical parameters.

Other field  data have been collected by researchers associated with the Louisiana Universities
Marine Consortium (LUMCON) and Gulf Coast research institutions and universities which have
been sampling the Gulf of Mexico for many years.   Data  from the  Gulf  of Mexico Hypoxia
Monitoring  Survey QAPP (Greene, 2003; Greene, 2007) will be subject to the QA procedures
specified in those documents. Data  received from other reliable sources will  be subject to QA
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procedures similar to those required for the Monitoring Survey.  Details of QA can be found in
Section A.7.

 B.4 Model Confirmation

Model confirmation is the process of reviewing the physical aspects of the model to ensure they
match the proposed processes.  Model confirmation includes reviewing the model equations for
appropriateness to the physical, chemical, and biological attributes of the system under study
and  for conformity to established theory;  reviewing model computer code to verify that model
equations have been accurately implemented; and testing of completed individual code modules
for functionality.

A  mathematical model consists of  differential equations representing physical, chemical,  or
biological processes in the system of interest.  When a model is selected or designed for a
natural system, the proposed equations should be reviewed to make sure they appropriately
represent the system.  The  proposed model for  this project is  based upon models  using
accepted  formulations for eutrophication kinetics that have  been  successfully  applied  to
simulate eutrophication in estuarine (Cerco and Cole,  1994), saltwater (Hall and Dortch, 1994),
and  freshwater systems (Pauer et a/., 2006).  Scientific  studies from the Gulf of Mexico
Louisiana-Texas shelf area will be reviewed to  confirm that the  proposed  chemical and
biological kinetics are appropriate for describing processes in this region.  If new equations are
required to describe processes not presently included in this model, they will be developed
based upon peer-reviewed scientific studies. The model construction, including the equations,
will also undergo an informal internal and external peer review process by scientific experts with
experience in the Gulf of Mexico to confirm its appropriateness for this project.  Further peer
review is  obtained  during the publication process in a peer reviewed  scientific/engineering
journal.

The  Gulf  of  Mexico hypoxia model will be  based  upon  the LM3-Eutro  model,  which has
undergone extensive testing, code review, and formal peer review (Pauer et a/., 2006; Melendez
et a/., 2009).  This original code will not require further  review.

New equations  or changes to  existing model code will undergo a rigorous review process. The
programmer responsible for translating model equations into code will  provide an initial review.
The  originating personnel providing the initial equations will also conduct a review of the code to
confirm that proposed equations or changes have been correctly implemented.  Finally, model
code will be available for review during any peer review process. All changes to model code will
be documented and tracked through the RCS system (see Section B.8).

Any  revised  or new  code will be  tested to  ensure  it correctly calculates  the  embedded
equations.  The output of any module that is revised or newly added will be tested before
including the module  in the  overall computer model.  Output will  be compared to  hand-
calculated and/or spreadsheet derived analytic solutions and to results from previous versions.
Sensitivity analyses will be used to confirm that the module is calculating correctly.

The  revised model will be tested to ensure that fundamental  operations, such as continuity and
mass conservation,  are verified.   Tests will  include checking  of  numerical stability and
convergence properties of model code algorithms, if appropriate.  Model results will be checked
by comparing  results  to  those  obtained by other  models and  by comparison  to  manual
calculations.  Visualization of model results and statistical correlations to field data will assist in
determining whether model simulations are realistic.
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 B.5 Model Calibration and Corroboration

Calibration is "the process of adjusting model parameters within physically defensible ranges
until the resulting predictions give the best possible fit to observed data" (USEPA, 2009).  The
model  calibration will  be accepted when the model simulates the majority of the cruise data
within  the  95% confidence intervals (+/-  two standard  errors)  for  important  measures  of
constituents such as minimum dissolved oxygen concentrations, area and duration of hypoxia,
and concentrations of nutrients and phytoplankton.  It is expected that the model fit to data will
be better than this criteria in many instances. For important constituents, the calibrated model
should also have a significant correlation at the 95% confidence level to field  data stratified
appropriately in time and space.

Calibration of the proposed  Gulf of  Mexico  hypoxia model  will  be conducted following a
systematic procedure. Initial parameterization will be accomplished as described in Section B.3.
Model  parameters will  be individually  adjusted  to  determine the sensitivity  of the  model
simulation results to each parameter.  Parameters will only be adjusted within ranges obtained
through project studies or published in the literature.  Model calibration will start with the most
conservative  constituents and  proceed  through  constituents  that  depend  on previously
calibrated constituents.  Calibration efforts will focus on parameters with the largest uncertainty
and upon which model results have the largest sensitivity. The model will be calibrated against
a one-year data set from the 2003 to 2007 monitoring program  data collected by EPA's Office of
Research  and  Development  Gulf Breeze laboratory.  Methods of calculating  or estimating
loadings or other forcing functions may be refined,  if necessary, but no calibration  of forcing
functions will  be allowed. The calibration will proceed until an optimal fit to data is achieved for
all important constituents.  Goodness-of-fit will be assessed by qualitative comparison of model
results to data  plots as  well as by the  quantitative statistical tests described in  the  preceding
paragraph.

There is an attempt within this document to help managers determine the degree to  which the
models will be calibrated to field data.   This constitutes the project acceptance criteria and
reflects what can practically be done with the resource commitments. The criteria for accepting
the modeling  results lies in the ability to simulate measured concentrations of materials in water,
sediment,  and biota during the field collection period.  If this is done within the statistical range
required, then the model(s) can be used to extrapolate these concentrations  in space and time.

Model  corroboration, also called validation, includes the "quantitative and qualitative methods
for  evaluating the degree to which  a model corresponds to reality" (USEPA,  2009).   The
calibrated model  will  be considered corroborated if model results for important constituents,
generated using a second independent set of inputs, fall within the 95% confidence intervals of
most of the data cruise means from the second data set.  Corroboration will focus on important
measures of constituents such as minimum dissolved oxygen concentrations, area and duration
of hypoxia, nutrient concentrations, and phytoplankton concentrations.

For corroborating the  model, the calibrated model will be compared against one year of data
from the 2003-2007 GED monitoring program independent of the calibration data set.   Initial
conditions  and  external  loadings will be obtained from the same independent data  set.  The
model will be run using  these inputs, and model results will be compared against data means
and  confidence intervals.  The  data  from  the calibration and corroboration  years will be
compared to  available long-term data sets to determine if these years were representative of
typical  conditions.  Goodness-of-fit will be assessed by qualitative comparison of model results
to data plots as well as by quantitative statistical tests.
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 B.6 Model Sensitivity/Uncertainty Analysis

The sensitivity of the model to individual model parameters will be evaluated informally as part
of model calibration.  Parameters will be adjusted one at a time and model results reviewed to
determine the sensitivity of the  model to  the adjustment.   Model calibration will  focus on  the
most sensitive and uncertain parameter values.

After the  model is calibrated  and  corroborated,  a more  formal  sensitivity analysis will  be
conducted. Individual parameters will be varied by a specified percentage as a measure of the
sensitivity of the  model to each  parameter  and  the change  in  model results  recorded.
Parameterization error can  be a significant source of model prediction uncertainty.  Statistical
measures of uncertainty in the input data will be reviewed. This review will not only include data
used for model initialization, calibration, and corroboration but also the uncertainty in tributary
and atmospheric loading estimates and in the hydrodynamic predictions.

To evaluate and  quantify the  effects of  parameterization  error,  uncertainty  analysis will be
performed for selected model simulations.  A statistical procedure to estimate uncertainty, such
as the  parameter variance-covariance estimation procedure of Di Toro and Parkerton (1993),
and/or  Bayesian Monte  Carlo,  propagation of error, and other statistical techniques will be
applied to estimate data, parameter, and model error components.

The uncertainty in forecast predictions is higher than  in simulations of present conditions due to
the higher uncertainty in predicted inputs.   For forecast  predictions, the model will be run with
inputs,  boundary conditions, and process rates bracketed in terms of extreme expectations and
probability distributions.   The  results will  be provided in terms of  prediction  means and
exceedance limits.

Model  results will also be qualified according to any explicit and  implied assumptions made in
developing or applying the  model.   Managers  will have to decide whether or not to use  the
model  results and  whether  or not to conduct additional  research to improve the models.  The
modelers can advise management on areas of input that have high uncertainty and to which  the
model  is very sensitive to.  Of  course, cost will  also be taken into  consideration.  This is a
continuing process.

 B.7 Model Application

After the  model is calibrated  and  corroborated, it will be  applied to  assist in answering
management questions and to provide insight into physical,  biological, and chemical processes
affecting hypoxia in the Gulf of Mexico. The model will be run using inputs developed according
to selected nutrient loading scenarios.  Management questions will include running the model to
review  the reduction in nutrient loads required to reduce the area of hypoxia to 5000 km2, a goal
specified in the 2001 Hypoxia Action Plan  (Mississippi River/Gulf of Mexico Watershed Nutrient
Task Force, 2001).  Subsequent scientific reviews have  suggested that phosphorus reductions
may also be necessary to reduce the area of hypoxia, and additional loading scenarios may be
run to  determine  the model  response to varying loads  of both phosphorus and  nitrogen.
Additional scenarios may be specified by project managers or peer review members during  the
project.  The  model is not designed to  make predictions on the effects of intensive,  short
duration events, such as a hurricane.

Model  output will be presented both graphically and  in tabular form.  The area  and duration of
hypoxia will be  important measures as  well as nutrient and  phytoplankton  concentrations.
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Scenario results will include comparisons  to  base line,  target,  and other scenarios.   An
animation tool will be developed to present time series results of model scenarios for the area of
interest.

 B.8 Data Management

All  records, including  modelers' electronic files,  will  be  maintained according  to Agency
standards as defined by the USEPA Office of Information Resources Management Federal
Information Processing  Standards (FIPS).   Paper  notebooks  will be  issued to modelers that
meet ORD  paper notebook guidelines.  Notes  in these books are of secondary importance to
documentation that will  exist in electronic form.  Minimum requirements for documenting and
maintaining ORD paper notebooks  are covered in  Chapter 13.2 - Paper  Laboratory Records
(12/01/2006)     of     the    ORD    Policies    and     Procedures    Manual     at:
http://dcordhqapps1.epa. gov:9876/orma/policies.nsf/webPolicy?OpenView.     Many  of   the
records associated with the  project will be in the form of electronic mail  (email).  Email and
electronic records that are subject to both the Federal Records Act (FRA)  and the Freedom of
Information Act (FOIA) will be preserved within  EPA's Enterprise Content Management System
(ECMS). ECMS is  a NARA - approved electronic recordkeeping system.  These laboratory
notebooks and electronic  files will  be maintained  by each modeler and turned over  to  the
Principal Investigator upon completion of  the  project.   Similarly,  electronic files containing
documentation of model testing, calibration, and validation will be maintained by each modeler
and transferred to a central project archive as designated by the Principal Investigator.

The primary water quality and process studies  data used to support modeling activities  will be
obtained from the GED  Gulf of Mexico Hypoxia Study which has been subjected to an EPA QA
process. Secondary data from other studies, published and unpublished sources; other data of
opportunity; and  equations, kinetics,  process rates,  and coefficients routinely used in modeling
applications  can  vary in their extent of QA  examinations.  All  data, to the  extent possible,  will
undergo review as specified in Section A.7. A database tracking system has been instituted by
the LLRFRB (Large  Lakes and Rivers Forecasting  Research Branch), Grosse lie for modeling
systems.  This database  system will  be used to  provide  data entry, storage, access, and
analysis capabilities to  meet the needs of the modelers and  other potential users of Gulf of
Mexico data. The system employs a single contact person for data being received.  The contact
person logs in routine information about the data and coordinates its use. The process provides
updated versions if  changes occur within the database.  The second component of tracking
involves versions which  have been assessed and completed for modeling purposes. Currently,
this position is provided  through the Computer Sciences Corporation contract.

Development and production of  software code is  maintained at the LLFRB in the  Revision
Control  System (RCS).  RCS forces strict revision control, supports check-out, locking, and
check-in of individual program files for development, and maintains a history and documentation
on all changes made to each program file.  RCS allows the user to recover specific versions of
files so that they can be tested  and re-used  in the model.  Documentation associated with
modifications made to  files  is  stored in  RCS and  within  the  program file itself.   This
documentation helps the user/modeler recall or understand why changes were made to files
over the course of the source code development.   In order to  facilitate code maintenance and
readability,  standard programming style and code  documentation will be  followed (Melendez
and Griesmer, 2002; also see Section B.2).

An Operating Procedure will be made available to modelers of the project that will outline types
of model-related files that will be archived at the end of the project. At a minimum, sample input
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and output files,  key calibration and final runs, model source code, compiled source code with
compiler identified,  and any principal pre- and  post-processing programs that were used to
support the models will be archived.   Readme files will  be included to describe any critical
information on running the model. The guiding principle in model archiving is to save whatever
is necessary to  recreate the  supportive files and model  runs that have been selected.  For
extremely large output files, saving only the first part of the run and last part of the run would be
permissible in order to conserve disk space.  This  would be explained in an accompanying
readme file.   The  directory  structure for the  model archive should  be  hierarchical with
meaningful names given to folders.  A contiguous archival directory should be used for all of the
models applied to the project by a  given organization.  Please refer to Rygwelski (2005) for a
draft operating procedure for archiving models.
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              GROUP C: ASSESSMENT AND OVERSIGHT
 C.1 Assessments and Response Actions

There will be internal and external assessment of the model throughout development, including
the evaluation  stage.  Assessments  will be conducted  internally during the  project by the
modeling team  and project management staff.  Review of model structures and implementation
are described in Section  B.4.   Reviews will be documented in project notebooks and internal
memoranda with responses documented accordingly.  Project activities  will also be reviewed
and  assessed  during bi-weekly meetings of  the  modeling staff, with assessments  and
responses recorded in meeting minutes.

The external review will be conducted through a continuing informal review process.  Review by
one's peers is an essential component to  any successful  and  credible scientific/modeling
endeavor.  Model development and  application is a very complex process with many important
issues with multiple approaches available to address them.  The external reviews provide an
objective means to arrive at a scientific consensus as well as provide judgment on scientific
credibility.  Reviewers will include staff from the ORD/NHEERL Gulf  Ecology Division and the
Office of Environmental  Information,  Environmental Modeling and  Visualization  Laboratory.
Assessments will  be  performed on the  model  structure and code  (including the numerical
schemes used), estimations of model coefficients and forcing functions, test runs on  other
computer systems, and reasonableness of model results.

External   assessments   will   also  include   submission   of   articles  to  peer-reviewed
scientific/engineering journals.  Because this project is likely  to be assigned a QA Category
Level II  status, publication  in journals is a requirement.   Otherwise, a formal  external peer
review is required.

 C.2 Reports to Management

The  Principal Investigator will meet periodically  with the modeling team and provide periodic
email and verbal  communication to EPA management on model progress, questions,  and
changes to the  original modeling plan.
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                GROUP D: VALIDATION AND  USABILITY
 D.1 Model Review

The mathematical model will be evaluated to determine how well the model meets the specified
data objectives  and acceptance criteria (see  Section A.7).  These criteria include framework
evaluation, code verification,  numerical methods accuracy, validation of input data, calibration
and corroboration results, and appropriateness of model scenario results. This section contains
a summary of the criteria that will be used for checking and accepting data and model output.

The theoretical  and mathematical basis of model processes developed as part of this project
must be consistent with established scientific theories and modeling practices.  Any new model
code must accurately describe the theoretical  processes and be free from  typographical errors.
New code must be consistent with the numerical solution technique and not cause significant
numerical dispersion or rounding errors.

Project-generated data used for the model inputs or for comparison to model output must have
passed the monitoring QAPP and an additional modeler review.  Data from external sources
should preferentially have undergone a quality assurance process and will also be required to
pass a review by project modelers.

The model will be considered calibrated and corroborated when the  model  results are within the
95% confidence intervals of the majority of the data  cruise means for  important measures of
constituents  for the respective data sets.  For important constituents, the  calibrated model
should also  have a significant correlation at the 95% confidence level to field data, stratified
appropriately in time and  space.   Important  measures of constituents include  minimum
dissolved oxygen concentrations, area and duration of hypoxia, and concentrations of nutrients
and phytoplankton.

The model will be applied to selected loading scenarios to evaluate the effects of load on the
extent  and duration  of hypoxia in  the  Gulf  and  the effects  of reducing both nitrogen  and
phosphorus.  There is no comparative data set for these model runs because the input data are
hypothetical  predictions.  However, to be considered valid, all model scenarios and results must
be approved by  expert elicitation  provided by the project peer review process.

 D.2 Verification and Validation Methods

This section summarizes the methods that  will be  used to  assess and verify  that  criteria
summarized  in D.1 are met.  Full  descriptions of these procedures are contained  in Section B.

The  Gulf of Mexico  hypoxia model will be  based  upon existing models.  The majority of
theoretical basis, process formulation, numerical solution technique,  and model code have been
previously verified, passed a formal  peer panel review process, and published in peer-reviewed
scientific journals.  These portions  of the  model will  not require additional review.  Any new
processes added to the model will be based upon established scientific studies and must pass a
peer review process.  New model code will be  documented, verified, and checked for numerical
accuracy as  described in Section B.2 Model Coding and Section B.4 Model Confirmation.
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Project-generated data will only be used after it has passed the monitoring QAPP acceptance
criteria (Greene,  2003, 2007) and an additional review of suitability for modeling by the project
modeling team.  Data from other sources will preferentially be taken from sources that have
followed  a  QA-QC  process  or have undergone  peer review.  These data will  undergo an
additional review by the  project modeling team.   Additional details of the data review and
acceptance  procedure  is  included  in Section   A.7 Quality  Objectives  and  Criteria  for
Measurement Data and Section B.8 Data Management

The model will be calibrated and corroborated following the procedures listed in Section  B.5
Model  Calibration and Corroboration.   The calibration and  corroboration will be considered
acceptable when criteria listed in Sections D.land A.7 are met. Standard statistical tests will be
applied to compare model output with  appropriately averaged field data to determine if the
criteria have been met.

Applying  the model to run forecast scenarios is described in Section A.7 Quality Objectives and
Criteria for Measurement Data and in Section B.7 Model  Application.  Scenarios will include
those suggested by the management team to help evaluate restoration goals for the Mississippi
River/Atchafalaya River/Gulf of Mexico system. Because the scenarios involve predicted inputs,
there will not be field data for comparison to outputs.   Inputs will  be bracketed by developing
through expert elicitation likely minimum and maximum loads, and the results will  be reviewed
through the peer review process to ensure the reasonableness of predicted outputs.

 D.3 Reconciliation with User  Requirements

The accuracy and reliability of models were quantified in Sections A.7 (Quality Objectives and
Criteria for Measurement Data), B.5 (Model Calibration and Corroboration) and B.6  (Model
Sensitivity/Uncertainty Analysis).  The  specific Data Quality  Objective for this study was to
develop a modeling suite capable of  simulating nutrient concentrations in the Gulf of Mexico to
within two standard errors of the means of observed concentrations in the water column. These
estimates were based on other modeling studies  and the reported performance statistics of
equivalent modeling frameworks when applied to similar systems.  The modeling project leader
will meet regularly with  the  users  and  managers  to communicate the  status  of model
development including the model accuracy.  The project leader will also inform the users how
changes  in model accuracy will impact  the application and interpretation of the model results
when performing load reduction scenarios.
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Pauer, J.J.,  K.W. Taunt, and  W. Melendez. 2006.  LM3-Eutro.  In: Rossmann,  R. (Ed.), Results
   of the Lake Michigan Mass  Balance Study: PCBs  Modeling Report,  Part 2, pp. 120-182.
   U.S. Environmental  Protection Agency, Office of  Research and Development, National
   Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division,
   Large Lakes Research  Station, Grosse lie, Michigan. EPA-600/R-04/167, 579 pp.

Pauer, J.J., A.M. Anstead,  W. Melendez, R. Rossmann , K.W. Taunt, and  R.G. Kreis, Jr. 2008.
   The  Lake Michigan Eutrophication Model, LM3-Eutro: Model Development and Calibration.
   Water Environ. Res.  80(9): 853-861
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Gulf of Mexico Hypoxia Modeling RQAPP
Pauer, J.J., A.M. Anstead, W. Melendez, K.W. Taunt, and R.G. Kreis, Jr. 2011. Revisiting the
   Great Lakes Water Quality Agreement Phosphorus Targets  and Predicting  the Trophic
   Status of Lake  Michigan. J. Great Lakes Res.  37, pp. 26-32.

Pew Oceans Commission.  2003.  America's Living Oceans -  Charting a Course for Sea
   Change.  A Report to the  Nation - Recommendations for a New Ocean Policy.   Pew
   Oceans Commission, Arlington, Virginia.

Pineiro, G., S. Perelman, J.P. Guerschman, and J.M. Paruelo. 2008.  How to Evaluate Models:
   Observed vs. Predicted or Predicted vs. Observed? Ecol. Model.  216: 316-322.

Rabalais,  N.N.,  R.E.  Turner,  D. Justic,  Q.   Dortch,  and W.J. Wiseman,  Jr.    1999.
   Characterization of Hypoxia.  Topic 1:  Report for the Integrated Assessment on Hypoxia in
   the Gulf of  Mexico. U.S. Department of Commerce,  National Oceanic and  Atmospheric
   Administration, Coastal Ocean  Program, Silver Springs, Maryland.  NOAA Coastal Ocean
   Program Decision Analysis Series Number 15, 203 pp.

Rabalais, N.N.,  R.E. Turner, and W.J. Wiseman, Jr.  2001. Hypoxia in the Gulf of Mexico.  J.
   Environ. Qual.  30(2):320-329.

Rabalais, N.N.  and R. Turner (Eds.).   2001.   Coastal  Hypoxia:  Consequences for  Living
   Resources and Ecosystems. American. Geophysical  Union, Washington, DC., 454 pp.

Rabalais, N.N.,  R.E.  Turner, Q. Dortch,  D. Justic,  V.J. Bierman,  Jr. and  W.J. Wiseman, Jr.
   2002.  Nutrient-Enhanced Productivity in the  Northern Gulf of Mexico: Past,  Present and
   Future.  Hydrobiologia.  475/476(1 ):39-63.

Richardson, W.L,  D.D. Endicott, R.G. Kreis, Jr., and K.R. Rygwelski.  2004.  The Lake Michigan
   Mass  Balance Project:  Quality Assurance  Plan  for Mathematical Modeling.    U.S.
   Environmental  Protection Agency, Office of Research and Development,  National Health
   and  Environmental Effects  Research Laboratory,  Mid-Continent Ecology  Division-Duluth,
   Large Lakes Research Station, Michigan.  EPA-600/R-04/018, 233 pp.

Rygwelski, K.R. 2005. Draft, Operating Procedure for  U.S. EPA-Grosse  lie Model Archiving
   and Operating  Guidelines.  U.S. Environmental Protection Agency, Office of Research and
   Development,  National  Health  and  Environmental  Effects   Research Laboratory,  Mid-
   Continent Ecology Division-Duluth, Large  Lakes Research Station,  Grosse lie, Michigan.
   LLRS-MCM-OP-001,6pp.

Scavia, D.,  N.N. Rabalais, R.E.  Turner, D. Justic,  and W.J. Wiseman Jr.  2003. Predicting the
   Response of Gulf of  Mexico Hypoxia to Variations in Mississippi  River  Nitrogen  Load.
   Limnol. and Oceanogr.  48(3):951-956.

Scavia, D.,  D. Justic, and V.J. Bierman, Jr.  2004.  Reducing Hypoxia  in the  Gulf of Mexico:
   Advice from Three Models. Estuaries. 27:419-425.

Scavia, D.J. and K.A. Donnelly.  2007. Reassessing Hypoxia Forecasts  for the Gulf of Mexico.
   Environ. Sci. Technol.  41:8111-8117.

U.S. Commission on Ocean Policy.   2004.   An Ocean Blueprint  for the 21st  Century.
   Washington, DC.
September 26,2012                          38                                 Revision 1

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Gulf of Mexico Hypoxia Modeling RQAPP
U.S. Environmental  Protection Agency.   1991.   Quality Assurance Guidelines for Modeling
    Development and Application Projects: A Policy Statement. U.S. Environmental Protection
    Agency, Office of Research and Development, Environmental Research Laboratory, Duluth,
    Minnesota.

U.S. Environmental Protection Agency. 2000.  Guidelines for the Preparation of MED Research
    Plans.  U.S. Environmental  Protection Agency,  Office of Research and Development,
    National  Health  and Environmental  Effects  Laboratory, Mid-Continent  Ecology  Division,
    Duluth, Minnesota.

U.S. Environmental Protection Agency. 2002a. Guidance for Quality Assurance Project Plans.
    EPA QA/G-5. U.S. Environmental Protection Agency, Office of Environmental Information,
    Washington, D.C. EPA/240/R-02/009, 111 pp.

U.S. Environmental Protection Agency.  2002b. Guidance for Quality Assurance Project Plans
    for Modeling,  EPA QA/G-5M.    U.S.  Environmental  Protection  Agency, Office  of
    Environmental Information, Washington, D.C.  EPA/240/R-02/007, 121 pp.

U.S. Environmental Protection Agency. 2005.  The Graded Approach to Quality Assurance and
    Definitions of Research Categories.   In:  Quality Management Plan for the National  Health
    and   Environmental  Effects  Research  Laboratory  (NHEERL),  Attachment  D.    U.S.
    Environmental Protection Agency, Office of Research and Development, Research Triangle
    Park,     North     Carolina,     QMP-NHEERL/99-01-001,     55     pp.         URL:
    http://www.nheerl.epa.gov/administration/qa/files/qmp05_attachments.pdf

U.S.  Environmental  Protection  Agency.     2008a.    Integrated   Modeling for Integrated
    Environmental Decision-Making.  A White Paper by U.S. Environmental Protection Agency,
    Washington, D.C. EPA100/R-08/010,  58 pp.

U.S. Environmental  Protection  Agency  Science Advisory  Board.   2008b.   Hypoxia  in the
    Northern Gulf of Mexico. An Update by the EPA Science Advisory Board. Washington, DC.
    EPA Science Advisory Board, EPA-SAB-08-003.

U.S. Environmental Protection Agency.  2009. Guidance on the Development, Evaluation, and
    Application of Environmental Models.  U.S. Environmental Protection Agency, Office of the
    Science  Advisor,  Council for Regulatory  Environmental  Modeling,  Washington,  D.C.
    EPA/1 OO/K-09/003, 90 pp.

U.S. Environmental  Protection Agency.   2012.  Safe and  Sustainable Water Resources
    Strategic Action  Plan  2012-2016.    U.S. Environmental Protection Agency,  Office  of
    Research and Development, Washington, D.C.  EPA 601/R-12/004, 38 pp.

U.S. Geological Survey. 2006.  Real-time Streamflow and Water Quality (Mississippi River
    Basin Discharge to the Gulf). U.S.  Department of  the Interior, U.S. Geological Survey,
    Lafayette, Louisiana. URL: http://toxics.usgs.gov/hypoxia/mississippi/real time.html.

World Resources Institute.   2008.  Eutrophication and  Hypoxia in Coastal  Areas:  A  Global
    Assessment of the State of  Knowledge.  World  Resources Institute Policy Note:  Water
    Quality: Eutrophication and Hypoxia, No 1.  Washington, DC.
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Gulf of Mexico Hypoxia Modeling RQAPP
                                FIGURES
          MED Gulf of Mexico Hypoxia
          Modeling. Principal Investigator
          Russell G. Kreis, Jr., EPA
  MED Modeling Team
  Kenneth Rygwelski, EPA-
  Modeling Facilitator/Coordinator

  Amy Anstead, ICF International
  Phillip DePetro, ICF International
  Timothy Feist .Trinity Engineering
  Wilson Melendez, CSC
  James Pauer, EPA
  Mark Rowe, EPA
  Xiaomi Zhang, Trinity Engineering
           Hypoxia Modeling Project
           Collaborators (See Figure 2)
    Model Programming
    Wilson Melendez, CSC
    Data Management for Modeling
    David Griesmer, CSC
    Xiangsheng Xia, CSC
Figure 1. MED Gulf of Mexico Hypoxia Modeling Organization
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Gulf of Mexico Hypoxia Modeling RQAPP
          _L
     USER A Region 4
                                     USER A Office of
                                         Water
                                      M ississi ppirtS ulf of M e*i co
                                        Nutrient Task Force
                                     USER A Office of
                                    Wetlands, Oceans
                                     and Watersheds
 USEPAGulfof
Mesdco Program
    Office
                                                  USER A Region 6
                             USEPA

                          ORD.NHEERL

                           Gulf Ecology
                             Division
                          USEPA

                       ORD.NHEERL

                        Mid-Continent
                       Ecology Division
                                         USEPA

                              Office of Environmental Information

                            Environmental Modeling and Visualization
                                       Laboratory
     USEPA

    ORD.NERL

Atmospheric Modeling
     Division
Figure 2.  Overview of Project Clients and Collaborators (with lines of communication
       only)
September 26, 2012
                    42
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Gulf of Mexico Hypoxia Modeling RQAPP
             Louisiana Inner Shelf
                  Study Area
Figure 3. Gulf of Mexico Study Area
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Gulf of Mexico Hypoxia Modeling RQAPP
 30WN
 29'0'O-N-
 MWN-
                                                Bottom Oxygen (mg/L)
                                                IMM
                                                • H>9h 9

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                                                 • Surions
              9«WW
93'0'0-W
92'0'0"W
              9ro'0"W
                              Areal Extent of 2007 Hypoxic Zone
                                Data courtesy of N. Rabalais and A. Sapp
Figure 4. Areal Extent of 2007 Hypoxic Zone
90*0'O'W
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Gulf of Mexico Hypoxia Modeling RQAPP
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Figure 5. Changes in Areal Extent of 1985-2008 Hypoxic Zone
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Gulf of Mexico Hypoxia Modeling RQAPP
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                                          Calendar Year
                                                 1955-1996 loads from Goolsby et al., 1999
                                                 1997-2007 loads from Aulenbach et al., 2007
Figure 6.  Annual Nitrate Load to the Gulf of Mexico
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Gulf of Mexico Hypoxia Modeling RQAPP
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Figure 7. Total Annual Phosphorus Load to the Gulf of Mexico
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Gulf of Mexico Hypoxia Modeling RQAPP
  hydrodynamic
     model
   (EPACOM)
atmospheric
  model
 (CMAQ)
other external
loadings and
 exchanges

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settling of
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Figure 9. Sediment-Water Interactions
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Gulf of Mexico Hypoxia Modeling RQAPP
                                 TABLES
Table 1.  Overall Project Schedule
MED Major Gulf of Mexico Project Activities
Project planning
Database development/management
Hydrodynamic model (EPACOM) incorporation into 3-D GoMDOM
Incorporate CMAQ atmospheric nitrogen fluxes into GoMDOM models
1-D GoMDOM model development
1-D GoMDOM model calibration
1-D GoMDOM model calibration / sensitivity / scenarios
1-D GoMDOM model journal article preparation
3-D 6km x 6km GoMDOM model development
3-D 6km x 6km GoMDOM model calibration
3-D 6km x 6km GoMDOM model corroboration
3-D 6km x 6km GoMDOM model management scenarios / sensitivity
3-D 6km x 6km GoMDOM model journal article preparation
3-D 6km x 6km GoMDOM model climate change scenarios
3-D 2km x 2km GoMDOM model parallelization
Fiscal Years
07 08 09 10 11
12
13
x x
X X X X
X
X
XXX
X
X

X X
X
X

X X X X
X
X
X
X


X

X
X

X
X
X


X
X
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Gulf of Mexico Hypoxia Modeling RQAPP
Table 2. List of Desired Field Measurements

Total Nitrogen
Dissolved Inorganic Nitrate
Dissolved Inorganic Nitrite
Dissolved Inorganic Ammonium
Particulate Organic Nitrogen
Total Kjeldahl Nitrogen
Total Phosphorus
Dissolved Inorganic Phosphorus
Particulate Organic Phosphorus
Dissolved Inorganic Silica
Total Organic Carbon
Dissolved Organic Carbon
Particulate Organic Carbon
Total Suspended Solids 0.7um
Conductivity
Salinity
Chloride
PH
Alkalinity
Transmissivity
Temperature
Wind Speed / Direction
Dissolved Oxygen
Photosynthetically Active Radiation
(400-700 nm)
Incident PAR
Light Extinction
Chlorophyll Fluorescence
Fast Repetition Rate Fluorometry
(Fluorometry/Productivity)
Phyto Biomass HPLC Pigments
Phyto Biovolume Size Fraction
Atmosphere
Wet Dry Gas
X


X
X
X
X
X

X
X


X
X
X
X
X
X











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X
X

X



X




X
X













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X





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X
















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X
X
X
X
X
X
X
X
X
X

X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
Tributaries
X
X
X
X
X
X
X
X
X
X

X
X
X
X
X
X
X
X
X
X

X



X
X
X
X
Sediment
X


X
X
X
X

X

X





X













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Error! Reference source not found, (continued).

C-14 Primary Productivity
Zooplankton Biovolume
Secondary Productivity
Microbial Productivity
Plankton Oxygen Demand
Porosity
% Water
% Solids
Reduction Oxidation Potential
Porewater
Sediment Oxygen Demand
Sediment-Water Nitrogen Flux
Atmosphere
Wet Dry Gas




































Gulf
X
X
X
X
X







Tributaries
X
X
X
X
X







Sediment





X
X
X
X
X
X
X
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Gulf of Mexico Hypoxia Modeling RQAPP
    APPENDIX 1:  CONCEPTUAL EQUATIONS FOR DISSOLVED
                 OXYGEN AND SEDIMENT DIAGENESIS
The Gulf of Mexico hypoxia modeling framework, GoMDOM, is based on the eutrophication
model, LM3-Eutro, which was developed for Lake Michigan (Pauer etal. 2006, 2008, 2011).
This document describes equations added-to or changed from those in the LM3-Eutro model.
There were several major changes to the LM3-Eutro model.  Dissolved oxygen (DO) was added
as a state variable with its transformation reactions.  Denitrification equations are also briefly
described which indirectly affect the DO in the Gulf of Mexico. The GoMDOM model also uses
a somewhat different approach than LM3-Eutro to estimate the impact of solar radiation (light)
on algal production.  The Jassby and Platt equation was used to estimate the limitation of
primary production by available light (Jassby and Platt, 1976,  Lehrter et al 2009).  Light
attenuation was calculated using a site-specific relationship between light attenuation and
chlorophyll, particulate carbon and salinity. A sediment diagenesis and flux sub-model are
planned for the future.

DISSOLVED OXYGEN EQUATIONS (WATER COLUMN)

The sources of DO in the water column include algal photosynthesis and reaeration. DO sinks
in the water column include algal respiration, organic carbon oxidation (bacterial respiration),
and chemical oxygen demand (COD), mainly sulfide oxidation and nitrification. DO sinks in the
sediment will be discussed in the SEDIMENT-WATER INTERACTION section.

Phytoplankton photosynthesis (PHOTO) and respiration (RESP) - see equation B.4 in Section
B.1 of main document.
Phytoplankton generate dissolved oxygen (photosynthesis) when sufficient nutrients, sunlight,
and "warmth" (temperature) are available and consume oxygen as a result of respiration.
Several equations have been proposed to describe these processes ranging form very complex
to rather simplistic approaches.  We propose the following equation for these processes, which
is a simplification of the CE-QUAL-ICM (Cerco and Cole, 1995) equation.


      =  kg-kd  P.AOCR                                                     (1)

where
      P = phytoplankton carbon concentration (mass carbon-volume"1)
      DO = dissolved oxygen concentration (mass-volume"1)
      kg = phytoplankton growth coefficient (time"1)
      kd = respiration rate (time"1)
      AOCR = dissolved oxygen-to-carbon ratio in respiration (2.67 gO2-gC"1)

In general, the Arrhenius equation was used to calculate the effect of temperature on the many
reaction rate coefficients.

k(T) = k(opt) 6[T-T(opt)]                                                           (2)

where
      k(T) = rate coefficient at temperature, T
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       k(opt) = rate coefficient at optimum temperature, T(opt)
       0     = temperature correction constant

Dissolved organic carbon oxidation - see equation B.4.
Dissolved organic carbon (DOC), for the most part, is present in the water because of the
decomposition of phytoplankton, zooplankton, and detrital particulate organic carbon. In the
presence of oxygen, DOC can be oxidized to carbon dioxide (CO2), an important oxygen sink.
Similar to the CE-QUAL-ICM model, the equation can be written as
                                                                                   (3)
  dt       Ksres + DO

where
       Ksres = half-saturation cone, of DO required oxidation (mass-volume"1)
       Kdoc = DOC oxidation rate (time"1)

Chemical Oxygen Demand (COD) - see equation B.4.
Chemical oxygen demand is the concentration (oxygen equivalents) of reduced species in the
water that can be rapidly oxidized chemically (absence of microorganisms).  It is assumed that
the source of COD in the Gulf of Mexico is mainly due to sulfide released from the sediments.
Using an oxygen dependency, we can write the equation similar to CE-QUAL-ICM.
  dt      KsCOD + DO
                                                                                   (4)
where
       COD = chemical oxygen demand (mass O2 equivalents-volume"1)
       KSCOD = half-saturation concentration of COD (mass-volume"1)
       KCOD = COD oxidation rate (time"1)

Nitrification - See equation B.4.
Due to the nature of the nitrifiers, it is generally accepted that nitrification occurs much faster in
the oxic regions of the sediment-water interface than in the water column. Even at these slow
rates, nitrification can be important in a relatively deep water column.  Nitrification is typically
modeled in low oxygen systems as a double Monod equation with a dependency on both
oxygen and ammonia.

  dNH4      DO          NH4
  	  = 	 * 	 	 * A 1 ) * kxJTT                                       tC
    dt     KsDO + DO   Ks^ + NH4           4                                      \°


where
       KsDO = half saturation rate for DO (mass-volume"1)
       KsNH4 = half saturation rate for NH4 (mass-volume"1)
       f(T) = temperature function similar to other equations in the LM3-Eutro model
       kNH4 = maximum nitrification rate (mass-volume"1-day"1)

Reaeration - See equation B4
It is generally accepted that reaeration in estuaries is largely dependent on wind effects (e.g.
Chapra, 1997).  The general equation can be written as:
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              D0sat-D0                                                            (6)
    Ql                                                                               ^  '

where
       K| = oxygen mass-transfer velocity (length-time"1)
       A = surface area of the water body (area)

The Wanninkhof equation was used to calculate the oxygen mass-transfer velocity as follows:

               ( Sc Y-5
K1=0.108Ur l||J                                                              (7a)

where
       Sc = Schmidt number (dimensionless number used to characterize fluid flows)
       Uw = wind speed 10 meters above surface (length-time"1)

The equation can be simplified if a Schmidt number of 500 is used to represent the value of
oxygen in water.

Kj  = 0.0986U"4                                                                    (7b)

Denitrification
In the absence of oxygen, nitrate can act as an electron acceptor during the oxidation of organic
matter. This process, known as denitrification, affects nitrogen and carbon concentrations.
Similar to nitrification, it can be modeled via a double Monod function.


                                            • Kdoc                                   /«\
       ,
           Ksres+D0   Ksdemt.N03

where
       Ratedenit = denitrification rate (time"1)
       Ksres = half saturation cone, of DO for oxic respiration (mass.volume"1)
       Ksdenit = half-saturation cone, of nitrate for denitrification (mass.volume"1)
       ANOX = ratio of denitrification to oxic carbon respiration
       Kdoc = respiration rate of DOC (time"1)

The subsequent mass balances terms for carbon and nitrogen are as follows:
    dt


  dNO3
    dt

where
        = - Ratedenit • ANDC • DOC
       ANDC = mass nitrate-N reduced per mass DOC oxidized (mass N-mass C"1)
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Gulf of Mexico Hypoxia Modeling RQAPP
SEDIMENT-WATER INTERACTION

A common approach used to describe the interaction between the sediment and the water
column is to assume a net sedimentation (difference between sedimentation and resuspension)
of phytoplankton and detrital material  and to describe sediment kinetic reactions and transport
of nutrients, oxygen and carbon within and across the sediments.  This is known as sediment
diagenesis (defined as all chemical, physical, and biological modifications undergone by a
sediment after its initial deposition) or sediment flux modeling. The approach will be in large
part based (at least as a first approach) on the  work by Dl Toro and co-workers as described in
the CE-QUAL-ICM users guide (Cerco and Cole, 1995), the Chesapeake Bay project report
(Cerco and  Cole, 1994), The Chesapeake Bay Sediment Flux model report (Dl Toro and
Fitzpatrick,  1993), and the textbook "Sediment  Flux Modeling" (Dl Toro, 2001).  The sediment
flux model includes the following state variables: phosphorus, ammonia, nitrate, silica, and
sulfide.  Below is a discussion of each state variable and equations to describe their transport
and chemical conversions in the sediments and at the sediment-water interface.

Phosphorus flux
A schematic diagram of the transport  and kinetic reactions is shown in Figure A-1. To simplify
the model, a partition coefficient is used to account for phosphate (dissolved form) in the pore
water and attached to the solids.  Several other assumptions are also made:
   1 . The sediment consists of two layers, a thin oxic layer (< 1 mm) and a much thicker
      anoxic layer (-10 cm)
   2. Because the aerobic layer is much smaller than the anaerobic layer, the particulate
      organic phosphorus, POP, settles directly to the anaerobic layer
   3. Thus no  diagenesis occurs in this thin oxic layer
   4. The anoxic layer has a fixed (user specified) thickness
   5. The solids concentration (TSS) is constant within the sediments
   6. The particulate organic phosphorus consist of several reactivity classes ranging from a
      labile (highly reactive) to a refractory form

The phosphate mass balance in the oxic, H! and anoxic layers, H2 can be written as follows:

      d[P04  1 1
   H!  L   dt   JT = s [P04 0 ]-fdl[P04 1 ]T                                    (11a)

                  + co12  fp2[P04 2 ]T-fpl[P04  1 ]T

                  + KL12 fd2[P04  2 ]T-fdl[P04
d[po'  2 IT     ,   f  rPO  2-|  _f
             —   \jj-i f-t  JL o  JL V_/ A  ^      JL
     ^           12   P2 L   4    JT    i

             - KL12  fd2 [P04  2 ]T - fdl [P04

                   [P04 1 ]  - [P04  2
                                           pl
                     o>2
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where
       H! and H2 = thicknesses of sediment layers 1 and 2, respectively (length)
       [PO4(0)]T, [PO4(1)]T and [PO4(2)]T = total PO4 concentration (which
       includes the dissolved and solid sorbed fractions) of the overlaying
       water, for layers 1 and 2,  respectively (mass-area"1)
       fd1 and fd2 = dissolved fractions in layers 1 and 2, respectively
       fp1 and fp2 = particulate fractions in layers 1 and 2, respectively

       These fractions can be calculated by the following equations (assuming the porosity is
       approximately one)


       fdi = — -            fR = 1 - fdi                                      (12a)
             1 + nij  • TTj                                                              v   '


        fd2 = - - - -           fp2 = 1 - fd2                                    (12b)
              1 + m2 '712
where
       rrH, m2 =  the solids (mainly Fe3+) concentration in layers 1 and 2, respectively
       (mass-volume"1)
       TTL TT2 = partition coefficients in layer 1 and 2, respectively (volume- mass"1)
       KL12 = mass transfer coefficient between the aerobic and anaerobic
       layers (length-time"1)
       oo12 = particle mixing velocity between the aerobic and anaerobic
       layers (length-time"1)
       oo2 = burial velocity (length-time"1)
       JP = source of phosphate from diagenesis of particulate organic
       phosphorus, POP (mass-area"1 -time"1)  and it can be estimated as follows


       T  -Vk    a(T20).
       Jp - /1/JKPOP,itl
            i = l
       POPi = concentration of particulate organic phosphorus in reactivity
       class i (mass-area1)
       kpopj = first-order reaeration rate coefficient (time"1)
       0 = temperature correction constant
       s = surface mass transfer coefficient between sediment and water (length. time"1)

The depth of the aerobic layer, \\-\ can be determined by the following equation:
where
       DDO = diffusion coefficient in the aerobic layer (length2. time"1)
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For the participate organic phosphorus (POP) the mass balance equations can be written as
follows:


  H2 — — - = -kpopjGpop;  POP;H2 - (fl2POP; + fPOp,iJpop                                   (15)

where
       POPj = concentration of particulate organic phosphorus in reactivity
       class i (mass-area"1)
       kpopj = first-order reaction rate coefficient (time"1)
       0 = temperature coefficient
       T = temperature (°C)
       oo2 = sedimentation velocity (length-. time"1)
       JPOP = depositional flux of POP from the overlying water to the
       sediment (mass-area"1 -time"1)
           = fraction of JPOp that is in the ith G class
It is well known that the phosphate sediment fluxes (across the sediment-water interface) are
strongly affected by the water column DO concentration. In most systems this is caused by the
Fe3/Fe2 redox reaction. In moderate and high DO concentrations, the phosphate forms a
precipitate with iron, and thus a very small sediment phosphate release rate.  In contrast, below
a critical DO concentration, phosphate is released to the overlying water. This can be described
mathematically:

it, = K2  ATTPO                DO  >  DO   pn                                       (16)
  1     /     FlJ4,l                           cnt, PO4

where
       [DO]crit, po4 = critical DO concentration (mass. volume"1)
       ATT = enhanced sorption (in the oxic sediments)

Sulfide flux
A schematic diagram of the sulfide behavior in the sediments is shown in Figure A-2. In a
manner similar to phosphorus, we can write equations for sulfide. For dissolved sulfide, the only
difference is a reaction (oxidation) term in the oxic layer. The mass balance equation for sulfide
in the oxic layer (1) and anoxic layer (2), respectively, can be written as follows:

            T =
0 ]-fdl[s2-  1 ]                                   (17a)
                 KL12  fds2-  2
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                                   2  '  ~fPiL5    A JT                               (17b)
                  -KL12  fd2[s2-  2 1  -fdl[s2-  1  1
                     LI/  a/ |_       JT    ai |_       J

                  + co2  [S2~  1  1   - [S2~ 2  1   + J
                     2  L        JT    L        JT
where
                                                      T
                                                     2
                                                    sz~
       [S2"(0)]T, [S"2(1)]T and [S2"(2)]T = total sulfide concentration (which
       includes the dissolved and solid sorbed fractions) of the overlaying
       water, layer 1 and layer 2, respectively (g/m2)
       The reaction term, kHi, and oxygen flux term Js  can be written as follows:
       klHl =  kH2S,dlfdl + kH2S,plfpl  0H2S
                                           '•M,H,S,O,
       J    - r,     T  _ r,   Tl M   rr  I                                                0^)
        S2~
       The carbon diagenesis term, Jc, can be expressed as:


                                  2                                                    (20)
where
       kh2s,di = reaction rate constant for dissolved oxidation (time"1)
       kH2s,pi  = reaction rate constant for particulate oxidation (time"1)
       KM,H2s,o2 = scaling factor
       ao2,H2s = stoichiometric coefficient (a value of 2.67 mgO2-mgC"1) is used
       ac,N2 = stoichiometric coefficient (a value of 1.071 mgC-mgN) is used
       Jc = carbon diagenetic flux (massC-area"1-time"1)
       J[N2(9)] = nitrogen gas flux (massN-area"1-time"1)
       kpoc,i= reaction rate (time"1)

Silica flux
A schematic diagram of the silica transport and kinetic reactions in the sediments are shown in
Figure A-3.  The equations are similar to phosphorus and sulfide sediment diagenesis. The
mineralization of particulate silica is believed to be a chemical as opposed to a biochemical
(mediated by bacteria) reaction.  It has been determined that the rate of biogenic silica
dissolution is proportional to the  silica solubility deficit [Si]sat - [Si]aq, where [Si]aq  is the dissolved
silica concentration .

The rate of dissolved silica production , SSi can be written as follows:


SSI  = ksi esr PSI   S!sat-[Sl  aq]
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From this we can calculate JT2 and k2 and can be written as:


JT2 = ksi ej-20  Si sat H2                                                            (22)



k  _ k  A T-20 f                                                                    (23)
K  - K  °     Z
  2 -   Si Si     d2
The dissolved silica mass balance in the oxic layer (1) and the anoxic layer (2), respectively,
can be written as follows:
                     i  0]-fdl[Si 1]T                                           (24a)
co12 fp2[Si  2]T-fpl[Si

KL12 fd2[Si  2 ]T-fdl[Si 1) ]T

   [Si  1 ]T
               - o>2
      dfSi  2 1
   H2  L      JT=-k2H2[Si 2]T                                                (24b)
                 -o)12  fp2[Si  2 ]T-fpl[Si  1 ]T

                 -KL12  fd2[Si  2 ]T-fdl[Si  1  ]

                 - co2  [Si 1 ]  - [Si  2 ]   + JT2
where
       kSj = rate coefficient for silica dissolution rate (time"1)
       k2 = rate coefficient (time"1)
       PSI = particulate biogenic silica (mass. volume"1)

 Ammonia and nitrate fluxes
Different from the phosphorus, silica and sulfide, the dissolved nitrogen species are present only
in the pore water (no solids partitioning). Other than that, the equations are very similar as
before.  A schematic of the transport and kinetic reactions are shown in Figure A-4 for ammonia
and Figure A-5 for nitrate. The mass balance equations for ammonia and nitrate can be written
for the oxic layer (1) and anoxic layer (2) as:


    d[NH4 1 1                                                                    (25)
 H!   L   dt    J =  - k^ [NH4  l^-K™ [NH4   l]-[NH4  (                 (  ]

                 + KL12 [NH4  2 ]-[NH4  1  ]

    d[NH4  2 1           r        .,  r        ..
H2	J =  -KL12  [NH4  2]-[NH4  l]  + JN2
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    d[N03  1 1
    _L	-	=< = - kN03)1 [NO3  1 ]H! - KL01 [NO3  1  ] - [NO3  01  1               (26)

                + KL12  [NO3 2 ] - [NO3  1  ]  + S NO3
   d[N03 2             r        n
H2 -L_J	J = _ kN03,2 [N03  2 ]H2 - KL12  [N03  2 ] - [NO3


 where
        S[NO3] = is the source of nitrate from nitrification
  The mass balance for the particulate nitrogen can be written as follows:

                                                                                  (27)
  H2        = - kPOHi 9P0;  PON.H, - co.PON, + fPON^ JPON


 where
        The nitrogen diagenetic flux can be written as:

         JN = IXoN, epS? PON,H2                                               (28)
              i=l
        kNH4 = nitrification rate (time"1)
        S[NO3]= nitrate generated due to nitrification (mass-area"1 -time"1)

 Finally we can expressed the Sediment Oxygen Demand Flux (SOD - see equation B.4.) in
 marine systems as the sum total of the oxygen demand due to the oxidation of sulfide (CSOD)
 and nitrification (NSOD) within the sediments

        SOD = CSOD + NSOD                                                      (29)

 where
        CSOD and NSOD can be defined as:

 CSOD = a0^s  J0 - aow J [N2 g  ]  ^  $ +^ S$  + ^ $                        (30)



 NSOD = a0i>NH.  % 9-20  [NH4 1 ]                                           (3D
                    o

 where
        frox(s) = fraction of sulfide being oxidized
        fraq(s) = fraction of sulfide being loss due to mixing with the overlying water
        frbr(s) = fraction of sulfide being removed due to burial
        K2NH4,i = nitrification velocity (length. time"1)
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Gulf of Mexico Hypoxia Modeling RQAPP
                        WATER COLUMN
    o  r
    S  DC
    2
  HI

  0
  UJ
  CO

    UJ
             SURFACE MASS TRANSFER:   K|_Qf
PARTTnQNJNQ:


  PO4 + FeQQH


PARTICLE MIXING
     *12
              DIAGENESIS:
                       POP
  PO4 + FeQOH


SEOiME^fTATlON
     w2
                                      F«OOH=PO4
                                         DIFFUSION
                         PO,
                                      FeOOH«PO4
                V

             BURIAL

Figure A-1: Schematic diagram of the phosphorus transport and kinetic reactions in the
sediments.
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  o  T*
  CD  CC
  O  LU
  CC  >
  m  3
LU
S
5
LU
CO
CC  UJ
UJ  >'
<  5
                      WATER COLUMN
                                             1
                                   H2SCO)
             SURFACE MASS TRANSFER:  KLQ1  f
PARTntONiNG:
                                              FiS
REACTIONS:       H2S + 2 O2 -> H2SO4


FeS + 9/4  62 -> 1/2  Fe2Oa + H2SO4 • H2O
             PARTICLE MIXING
                         DIfTUSlON


                            KL12
             DIAGENESIS:

             2 CH2O + H2SO4 -> 2 CO2 + H2S + 2 H2O
              PARTITIONING:    H2S
              SEDIMENTATION
                                FeS
              BURIAL


Figure A-2: Transport and kinetic reactions of sulfide in the sediments
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    o  *-
  LU
  S
  O
    o  cc
    EC  IJJ
                         WATER COLUMN
                                               Si(0)
              SURFACE MASS TRANSF1H:
PARTmONINQ:


        SI  H-
PARTICli MIXING
     w12
KL12
                                  JPSI
              DIASENESS:     PSi
                             SI
SOLU1IUTY:
                              Si
              PARTmONINQ:

                       SI + FeOOH

              SEDIMENTATION
              BURIAL

Figure A-3: Silica transport and kinetics in the sediments
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Gulf of Mexico Hypoxia Modeling RQAPP
    o  *-

    5  OC
    o  ui
    ff  >
    til  <
    
                          WATER COLUMN
                                                 NH4{0}
               SURFACE MASS TRANSFER:  KL01




                                        JN1
                                               |NH4(1)
               DIAGENESIS:
 REACTION;
DIAGENESIS:
               REACTION:
                  PON
                 NH,
       KNH4,1

 NH4  -	-		^  NO3
                                 DIFRJSION:   K|_12
PON
                         NONE
NH,
Figure A-4. Ammonia transport and kinetic reactions in the sediments
September 26, 2012
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                                     Revision 1

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Gulf of Mexico Hypoxia Modeling RQAPP
    g  *-
    CD  C
    o  uj
    cc  >
    UJ  <
    <  -J
 LU

 1

 O
 UJ
 CD
    o  cc
    CC  111
    uu  >
                         WATER COLUMN
                                                NQ3(Q)
                SURFACE MASS TRANSFER: «L01




                                      KNH4,1
SOURCE:
REACTION:
               SOURCE:
REACTION:
NHj
                                                •N03<1)
N03
      KNO3,1

NO3   	*-  N2(g)
                            DIFFUSION:
                              »
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REFERENCES

Cerco, C. F. and T.M. Cole.  1994.  Three-Dimensional Eutrophication Model of Chesapeake
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Cerco, C.F. and T.M.  Cole.  1995.   User's Guide to the  CE-QUAL-ICM Three-Dimensional
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   Station:  Vicksburg, Mississippi.

Chapra, S.C.  1997. Surface Water-Quality Modeling, McGraw-Hill, New York, NY, pp844

Dl Toro, D.M.  and J. Fitzpatrick.  1993. Chesapeake Bay Sediment Flux Model, HydroQual Inc.
   Mahwah,  NJ Prepared for the  US  Army  Engineer  Waterways  Experiment Satiation,
   Vicksburg, MS

Dl Toro, D.M.  2001. Sediment Flux Modeling, John Wiley & Sons, Inc. New York, NY pp 624

Jassby, A. D. and T. Platt.  1976.  Mathematical formulation  of the relationship between
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Lehrter, J. C.,  M.M.  Murrell, and J.C. Kurtz. 2009.  Interactions between Freshwater Input,
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Pauer, J. J., K.W. Taunt, and W. Melendez. 2006. In: Results of the Lake Michigan  Mass
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   National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology
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Pauer, J. J., A.M. Anstead, W. Melendez, R. Rossmann, K.W. Taunt, and R.G. Kreis, Jr. 2008.
   The Lake Michigan Eutrophication Model,  LM3-Eutro: Model Development and  Calibration.
   Water Environ. Res. 80(9): 853-861

Pauer, J.J.,  A.M. Anstead, W. Melendez, K.W. Taunt, and R.G. Kreis, Jr. 2011.  Revisiting the
   Great Lakes Water Quality Agreement  Phosphorus Targets and Predicting the Trophic
   Status of Lake Michigan. J. Great Lakes Res. 37, pp. 26-32.
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