SB
                 UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
        rch 9, 1988
                          WASHINGTON, D.C, 20460
                                                                        OFFICE OF

                                                                    THE ADMINISTRATOR
Hon. Lee M. Thomas                             SAB-EIC-88-014
Administrator
U.S. Environmental Protection
  Agency
401 M Street, SW
Washington, D.C.  20460

Dear Mr. Thomas;

     The Drinking Water Subconraittee of the Science Advisory Board's Environ-
mental Health Committee has completed its independent scientific review of the
Draft Drinking Vteter Criteria Cocument for Xylenes developed by the Envirormen-
tal Criteria and Assessment Office (Office of Research and Development) for the
Office of Drinking Water, dated June 1987, and is pleased to transmit its
final report to you.  The Subcommittee reviewed the draft criteria document
at a public meeting in Washington, D.C. on October 8-9, 1987.

     In general, the draft criteria document represents an unprovOTient over a
previous draft prepared by Agency staff.  It includes an informative survey of
the existing scientific literature for xylenes and appropriately discusses a
range of scientific issues related to toxieity and exposure.

     The primary issue in the review concerns the selection of studies used in
determining the Drinking Water Equivalent Level (EWEL).  The EWEL is based upon
the experimental level below which a health effect is not observed and provides
a non-carcinogenic basis for establishing a drinking water standard.  The Subcom-
mittee concludes that the Office of Drinking Water has selected the appropriate
studies to calculate the CWEL, and that the calculation was developed in a scien-
tifically supportable manner.

     The Subcommittee recommends that taste and odor should be a scientific basis
for a secondary Maximum Contaminant Level because most of the public will not
drink water that smells.  Discussion of this and other issues related to the
draft criteria document are presented in the attached report.

     We appreciate the opportunity to conduct this particular scientific review
and request that the Agency formally respond to our scientific advice.

                                              Sincerely,
                                                         n Nelson, Chairman
                                                    Executive Corenittee
                                                     ichard A. Gfiesemer, Chairman
                                                    Environmental  Health Conroittee
                                                    Gary #. Carlson, Chairman
                                                    Drinking Water Subcommittee

-------
                Review of the Draft Drinking Pfetter Criteria Document
                                   for Xylenes
                         by the crinking Water Subcommittee
                           Environmental Baalth Committee
                               Science Advisory Board

      On October 8-9,  1987 the Drinking Water Subcommittee  of  the Environmental
 Health Committee of EPA's Science Advisory Board reviewed  the Draft Drinking
 Water Criteria Document  for Xylenes  developed by the Environmental Criteria and
 Assessment Office {Office of Research and  Development)  for the Office of  Drinking
 Water,  dated June 1987.   The Subcommittee's major conclusions and recommendations
 are presented  below.

 Major Conclusions^ and _R.ecommendations

      1-  In general, the  draft criteria document represents an improvement
 over the previous draft.   It discusses the literature reasonably well.  The
 sections on absorption,  distribution, metabolism and excretion are thorough
 and convincing,  while the discussion of human effects appropriately points
 out the  difficulty associated with mixed exposures and  the exposure estima-
 tion.   The evaluation of developmental toxieity,  comparing the effects seen
 at  high  doses  in both the mother and the fetus with  the absence of effects
 observed at lower doses,  is balanced.

      2.  The primary issue in the review of this draft document concerns the
 selection  of studies  used in determining the Drinking water Equivalent level
 (EWEL).  The EWEL is  based upon  the  experimental  level  below  which a health
 effect  is  not  observed and provides  the non-carcinogenic basis for estab-
 lishing  a  drinking water standard.   In the health advisory document, previously
 reviewed by the  Environmental Health Committee in a  report dated October  24,
 1986, the  [WEL was based on an inhalation  study conducted  by  Jenkins,  et.  al»,
 in  rats, guinea  pigs, monkeys and dogs.  This new document uses data from the
 National Toxicology Program (NTP)  studies  in rats.   Data from the subchronic
 study  (No-ObservedEffeet-level [NOEL]  = 500 mg/kg/day)  are employed in deriv-
 ing  the  long-term health advisory, and results from  the chronic study (NGELr
 250 rog/kg/day) are utilized in the calculation of the CWEL.

     3.  Although  it is somewhat  surprising that the  rats were not wore sus-
 ceptible to xylene exposure,  the utilization of the  NTP results is accept-
 able, particularly because the route of administration  was oral.   There is
 uncertainty because xylene was administered as a bolus  dose in corn oil
 rather than in water, but the limited solubility of  xylene in water prevent-
 ed administration in  this manner.  The use of the data  is  also consistent with
 the  reccnroendation made  by the Environmental Health  Committee when it previous-
 ly reviewed the xylene health advisory.

     4,  The calculations  appear  accurate and follow  the usual methods for their
application.   The  safety  factor  of 100  is  appropriate in view of the criteria
set  for  selection  based upon the length of the study which, in this case,  is a
chronic  study  in animals.   The IWEL  of  63  mg/liter results in a substantial increase
over the previously proposed  value of  2,2  rog/liter based on Jenkin's inhalation
studies.   However, the obvious differences in experimental design,  and  the pre-
viously used formula,  may have been  conservative  in  estimating a respiratory

-------
                             -2-


volume of 20 m3 per day.  Also, there is the simple matter of a ten-fold
difference in the safety factor.

     5. The fact that the taste and odor value may be in the range of 0.3 to
1.0 ing/liter is important.  The Subcommittee reccrcroends that taste and odor
should also be a scientific basis for a regulatory decision by establishing
a secondary Maximum Contaminant level (MCL) because most of the public will
not drink water that smells.  Also, xylene can serve as a sentinel contamin-
ant for other components found in the same compound such as ethylbenzene,

     6. The draft document is inconsistent in stating that the longer-term
health advisory for the 10 kilogram child is 36 mg/liter compared to the
DWEL of 63 mg/liter.  This needs to be explained carefully to avoid misunder-
standing.  The inconsistency may be related to the use of the lower weight for
the child.

     7. The Subccnmittee agrees with the use of the longer-term health advisory
number for the 1 and 10 day health advisories*  The calculations previously
used for the 1 day advisory had some advantage in that they were based on
studies conducted in humans.  However, these were inhalation studies that may
not be as good a predictor for the effects of oral administration of "this ccm-
pound as previously thought.  Furthermorer the use of the previous 1 day health
advisory would lead to a discrepancy in that it would be lower than the longer-
term advisory.

     8. Seme scientific problens with the draft document remain, however.
These include:

        o The information likely to be in Chapter IV (not currently included
          in the document) for comparing the contributions from various
          sources is necessary for preparing an adequate analysis*  The Sub-
          committee cannot endorse the document as written due to this
          deficiency.  The- information in this chapter on exposure is essen-
          tial to making a judgment about the public health risk from xylenes.

        o The Drinking Water Equivalent Level that has been suggested for
          xylene is 60 mg/liter.  At this level, an individual can have
          significant intake of xylene because of inhalation and dermal
          exposure.  For example, if an individual took a 30 minute shower
          in an 18 m.3 room with a flow rate of 4 gallons of water per minute
          and half of the xylene volatilized, he/she would absorb about two
          times the amount (assuming 50% absorption through the lungs)
          ingested from 2 liters of water.  The calculation of the Maximum
          Contaminant Level Goal should consider these exposures; however,
          they should be confused with the exposures that occur through back-
          ground levels of xylene through other pathways such as air and food.

        o The Subcommittee has a question with regard to the statement that
          the deaths that did occur among the rats in the high dose group in
          the NTP study result from errors in gavage administration, although

-------
  "a behavioral effect may have caused the rats to resist dosing."
  Although this or a similar phrase is used several times in the
  draft criteria document, it is not at all clear what it means.
  Does it mean that there was some alteration in the behavior as
  a result of central nervous system perturbation suggesting that
  this level of exposure to xylene may have serious neurotoxic
  effects, or does it simply mean that rats learned to hate the
  stuff?  The Subcommittee recommends that the Agency clarify this
  point with the laboratory that conducted the NTP study,

o Ihe classification of xylene in Group D ("Not Classified as to
  Human Carcinogenicity") of EPA's risk assessment guidelines for
  carcinogenicity is inconsistent with the categories given on
  page VIII-4 of the draft document.  In view of the fact that
  the NTP found xylene to be negative in both rats and mice,
  EPA should also consider classifying it in Group E ("Evidence
  of Non-Carcinogenicity in Bumans") which uses the criteria of
  the lack of evidence in at least two adequate animal tests in
  different species.  Ihe Agency needs to clarify its scientific
  rationale for not accepting the NTP studies as evidence for
  this category.

o Ihe Subcommittee reeoranends a greater discussion in the draft
  document of the possible role that the use of a mixture in
  the NTP study may play in the attribution of the toxicity to
  any particular isoner of ethylbenzene.  This may also need to
  be considered in the ethylbenzene criteria document, depending
  on the results of studies with that compound,

o There is constant and unnecessary reptetition within the draft
  document.  Also, many of the papers reviewed are essentially
  irrelevant to making risk assessment judgments.

-------
                  U.S. Environmental  Protection  Agency
                         Science Advisory Board
                      Drinking Water Subcommittee

                       CHAIRMAN AND VICE CHAIRMAN

 Dr.  Gary Carlson, Chair, Department of Pharmacology  and Toxicology, School
 of Pharmacy; Purdue University, West Lafayette,  Indiana 47907

 Dr.  Robert Tardiff, Vice-Chair, Principal, Environ Corporation, 1000 Potomac
 St., N.W., Terrace Level, Washington, DC  20007

                         MEMBERS AND CONSULTANTS

 Dr.  Julian B. Andelman, Graduate School  of Public Health,  130 Oesoto Street,
 Parran Hall - Room A-711, University of  Pittsburgh,  Pittsburgh, PA  15261

 Dr.  Rose Dagirmanjian, Professor, Department of  Pharmacology and Toxicology,
 University of Louisville, Louisville, Kentucky  40292

 *Mr»  Jerome B, Gilbert, Manager, East Bay Municipal  Utility District, 2130
 Adeline Street, Oakland, California  94623

 *0r.  William Slaze, Director, School  of  Public Health, University of California,
 Los  Angeles, 650 Circle Drive South,  Los Angeles, CA 90024

 Dr.  J, Donald Johnson, Professor, School of Public Health, University of
 North Carolina, Chapel Hill, NC  27514

 *Dr. Marshall Johnson, Professor, Department of  Anatomy, Jefferson Medical
 College, 1020 Locust Street, Philadelphia, PA 19107

 Dr.  David Kaufman, Department of Pathology, University of  North Carolina,
 Rm.  515 Brinkhous-Bullitt,  Chapel Hill,  North Carolina 27514

 Dr.  Nancy Kim, Director, New York Department of  Health, Bureau of Toxic
 Substance Assessment,  Room 359, Tower Building,  Empire State Plaza, Albany,
 New  York  12037

 Mr. Richard Noser, Vice-President for Water Quality, American Water Works
 Service Company, 4001  Qreentree Executive Campus, Suite B, Mirlton, NJ
 08053

 *Dr. Betty Olson, Program in Social  Ecology, University of California,
 Irvine, CA  92717

 Dr. Verne Ray, Medical Research Laboratory, Pfitzer, Inc.  Groton, Connecticut,
 06340

 Dr. Harold Shechter, Professor, Chemistry Department, Ohio State University,
 140 West 18th Avenue,  Columbus, Ohio  43201

 Dr. Thomas Tephly, Professor, Department of Pharmacology,  The Bowen
 Science Bldg,, University of Iowa, Iowa  City, Iowa  52242

                              EXECUTIVE  SECRETARY

Dr. C. Richard Cothern, Executive Secretary, Science Advisory Board
 [A-101F] U.S. Environmental Protection  Agency,  Washington, D.C.  20460

 *Not present  at the meeting October 8-9, 1987

-------