Environmental Technology Verification

Test Report of Control of Bioaerosols in
HVAC Systems

Aeolus Corporation
Synthetic Minipleat V-Cell, SMV-M13-2424
              Prepared by
           Research Triangle Institute

           HRTI
           INTERNATIONAL
            Under a Contract with
         U.S. Environmental Protection Agency
           r&FPA
           mSK£ ^H I  f\
EIV EW  ET

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           THE ENVIRONMENTAL TECHNOLOGY VERIFICATION PROGRAM

 &EPA                                                        HRTI
                                                                            INTERNATIONAL
U.S. Environmental Protection Agency                                                      Research Triangle Institute
                       ETV Joint Verification Statement
        TECHNOLOGY TYPE:   VENTILATION MEDIA AIR FILTER

        APPLICATION:          FILTRATION EFFICIENCY OF BIOAEROSOLS IN
                                 HVAC SYSTEMS

        TECHNOLOGY NAME:  Synthetic Minipleat V-Cell, SMV-M13-2424

        COMPANY:             Aeolus Corporation

        ADDRESS:               6306A Old 421 Rd         PHONE: 336-622-5122
                                 Liberty, NC 27298         FAX:   (336) 622-5125
        WEB SITE:              http://www.aeoluscorp.com
        E-MAIL:                 billmatkins(a)msn.com
     The U.S. Environmental Protection Agency (EPA) has created the Environmental Technology
     Verification (ETV) Program to facilitate the deployment of innovative or improved
     environmental technologies through performance verification and dissemination of information.
     The goal of the ETV Program is to further environmental protection by substantially accelerating
     the acceptance and use of improved and cost-effective technologies. ETV seeks to achieve this
     goal by providing high quality, peer-reviewed data on technology performance to those involved
     in the design, distribution, financing, permitting, purchase, and use of environmental
     technologies.

     ETV works with recognized standards and testing organizations; stakeholder groups which
     consist of buyers, vendor organizations, permitters, and other interested parties; and with the full
     participation of individual technology developers. The program evaluates the performance of
     innovative and improved technologies by developing test plans that are responsive to the needs
     of stakeholders,  conducting field or laboratory tests (as appropriate), collecting and analyzing
     data, and preparing peer-reviewed reports.  All evaluations are conducted in accordance with
     rigorous quality assurance protocols to ensure that data of known and adequate quality are
     generated and that the results are defensible.

     EPA's National  Risk Management Research Laboratory contracted with the Research Triangle
     Institute (RTI) to establish a homeland-security-related ETV Program for products that clean
     ventilation  air. RTI evaluated the performance of ventilation air filters used in building heating,
     ventilation  and air-conditioning (HVAC) systems. This verification statement provides a
     summary of the  test results for the Aeolus Corporation Synthetic Minipleat V-Cell, SMV-M13-
     2424 media air filter.

                                           S-l

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VERIFICATION TEST DESCRIPTION
All tests were performed in accordance with RTFs "Test/Quality Assurance Project Plan:
Biological Testing of General Ventilation Filters," which was approved by EPA.  Tests were
performed for the following:
   •   Bioaerosol filtration efficiency tests of the clean and dust-loaded filter. Three bioaerosols
       were used in the testing:
          o   The spore form of the bacteria Bacillus atrophaeus (BG), a gram-positive spore-
              forming bacteria elliptically shaped with dimensions of 0.7 to 0.8 by 1 to 1.5 //m,
          o   Serratia marcescens, a rod-shaped gram-negative bacteria with a size of 0.5 to 0.8
              by 0.9 to 2.0 //m, and
          o   The bacterial virus (bacteriophage) MS2 dispersed as a micrometer-sized
              poly disperse aerosol.
   •   Inert aerosol filtration efficiency tests consisting of an American National Standards
       Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning
       Engineers (ASHRAE) Standard 52.2-1999 type test (0.3 to 10 |im) and extended
       fractional efficiency measurements down to 0.02 jim particle diameter on both clean and
       dust-loaded filter.
   •   ASHRAE 52.2 test. This test provides filtration efficiency results (average of the
       minimum composite efficiency) given for three size ranges of particles: El, 0.3 to 1.0
       //m; E2, 1.0 to 3.0 //m; and E3, 3.0 //m to 10 //m.

VERIFIED TECHNOLOGY DESCRIPTION
As shown in Figure  1, the Aeolus Corporation Synthetic
Minipleat V-Cell, SMV-M13-2424 media air filter is a V-
cell filter with  nominal dimensions of 0.61 by 0.61 by
0.31 m (24 by 24 by 12 in.).  The filter has a plastic frame,
and the filter media color is white and red stripes. The
media is polypropylene. There are four V-cells with
minipleated media.  The Aeolus Corporation part number
is  SMV-M13-2424.

VERIFICATION OF PERFORMANCE
Verification testing of the Aeolus Corporation Synthetic
Minipleat V-Cell, SMV-M13-2424 media air filter began
on September 9, 2003 at the test facilities of RTI and was
completed on October 8, 2003.  The results for the
bioaerosol filtration  efficiency tests are presented in  Figure 1. Photograph of the Aeolus Corporation
Table 1 for the clean and dust-loaded filter. Table 2           Synthetic Minipleat V-Cell,
presents the results of the ASHRAE 52.2 test. All            SMV-M13-2424 media filter.
tests were conducted at an air flow of 0.93 m3/sec (1970 cfm).
                                           S-2

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                             Table 1. Bioaerosol Filtration Results

Clean
Dust loaded
Pressure Drop
Pa (in. H2O)
77(0.31)
348(1.40)
Filtration
Efficiency for
Removal of
B. atrophaeus , %
69
99
Filtration
Efficiency for
Removal of
S. marcescens, %
64
99.5
Filtration
Efficiency for
Removal of
MS2 phage, %
73
99
                           Table 2. Summary of ASHRAE 52.2 Test

Aeolus Corporation
Synthetic Minipleat V-
Cell, SMV-M13-2424
El
0.3 to 1.0 Aim,
57
E2
1.0 to 3.0 Aim,
85
E3
3.0 to 10 Aim,
97
Minimum Efficiency
Reporting Value
(MERV)
12 at 0.93m3/sec
(1970 cfm)
The quality assurance officer reviewed the test results and the quality control data and concluded
that the data quality objectives given in the approved test/QA plan were attained.

This verification statement addresses two performance measures of media air filters: filtration
efficiency and pressure drop. Users of this technology may wish to consider other performance
parameters such as service life and cost when selecting a media air filter for bioaerosol control.
In accordance with the test/QA plan1, this verification statement is applicable to filters
manufactured from December 2003 through November 2006.
Original signed by E. Timothy Oppelt 12/8/2003
E. Timothy Oppelt                    Date
Director
National Homeland Security Research Center
Office of Research and Development
United States Environmental Protection Agency
Original signed by David S. Ensor 12/4/2003
David S. Ensor                    Date
Director
ETV-HS
Research Triangle Institute
   NOTICE: ETV verifications are based on an evaluation of technology performance under specific, predetermined
   criteria and the appropriate quality assurance procedures. EPA and RTI make no expressed or implied warranties
   as to the performance of the technology and do not certify that a technology will always operate as verified.  The
   end user is solely responsible for complying with any and all applicable federal, state, and local requirements.
   Mention of commercial product names does not imply endorsement.
                                             S-3

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Environmental Technology Verification
     Test Report of Filtration Efficiency of

         Bioaerosols in HVAC Systems


                    Aeolus Corporation
          Synthetic Minipleat V-Cell, SMV-M13-2424


                        Prepared by:

                    Research Triangle Institute
                   Engineering and Technology
                 Research Triangle Park, NC 27709
              GS10F0283K-BPA-1, EPA Task Order 1101
                    RTI Project No. 08787.001
                     EPA Project Manager:
                      Theodore G. Brna
              Air Pollution Prevention and Control Division
             National Risk Management Research Laboratory
                Office of Research and Development
                U.S. Environmental Protection Agency
                 Research Triangle Park, NC 27711
                       December 2003

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                                        Notice

This document was prepared by the Research Triangle Institute (RTI) with funding from the U.S.
Environmental Protection Agency (EPA) through the General Service Administration Contract
No. GS1OF0283K per EPA's BPA-1, Task Order 1101.  The document has undergone RTI and
EPA peer and administrative reviews and has been approved for publication. Mention of
corporation names, trade names, or commercial products does not constitute endorsement or
recommendation for use of specific products.

                                       Foreword

The Environmental Technology Verification (ETV) Program, established by the U.S.
Environmental Protection Agency (EPA), is designed to accelerate the development and
commercialization of new or improved environmental technologies through third-party
verification and reporting of performance.  The goal of the ETV Program is to verify the
performance of commercially ready environmental technologies through the evaluation of
objective and quality-assured data so that potential purchasers and permitters are provided with
an independent and credible assessment of the technology that they are buying or permitting.

EPA's National Risk Management Research Laboratory contracted with the Research Triangle
Institute (RTI) to establish a homeland-security related ETV Program for products that clean
ventilation air. RTI developed (and EPA approved) the "Test/Quality Assurance Plan for
Biological Testing of General Ventilation Filters1." The test described in this report was
conducted following this plan.
                                 Availability of Report

Copies of this verification report are available from

•      Research Triangle Institute
       Engineering and Technology Unit
       PO Box 12194
       Research Triangle Park, NC 27709-2194

•      U.S. Environmental Protection Agency
       Air Pollution Prevention and Control Division, E305-01
       109 T.W. Alexander Drive
       Research Triangle Park, NC 27711

Web sites:     http://www.epa.gov/etv/verifications
                                           11

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                                   Table of Contents

ETV Joint Verification Statement	S-l
Notice	ii
Foreword 	ii
Availability of Report	ii
Table of Contents	iii
Acronyms/Abbreviations	iv
Acknowledgments	v
1.0    Introduction	1
2.0    Product Description	1
3.0    Test Procedure	1
4.0    Test Results	4
5.0    Limitations and Applications	6
6.0    References	6
Appendix:  ASHRAE 52.2 Test Report	7
                                        Figures

Figure 1. Photograph of the Aeolus Corporation Synthetic Minipleat V-Cell,
       SMV-M13-2424 Media Filter	1
Figure 2. Schematic of Test Duct	2
Figure 3. Summary of the Inert Aerosol Filtration Efficiency Data for the Clean and
       Dust-Loaded Filter, # 2 	5
Figure A-l. Filtration Efficiency and Flow Resistance for Aeolus Corporation Synthetic
       Minipleat V-Cell,  SMV-M13-2424	10
                                         Tables

Table 1. Numbers of Filters and Expected Utilization	4
Table 2. Bioaerosol Filtration Results for Filter # 2	4
Table 3. Summary of Removal Efficiency Using ASHRAE 52.2 Test for Filter # 1	4
Table 4. DQOs for Precision of Filtration Efficiency Measurements for Culturable Bioaerosol... 6
                                           in

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                               Acronymns/Abbreviations
ANSI         American National Standards Institute
ASHRAE     American Society of Heating, Refrigerating and Air-Conditioning Engineers, Inc.
ASME        American Society of Mechanical Engineers
B             Bacillus
BG           Bacillus atrophaeus (formerly B. subtilis var niger and Bacillus globigif)
cfm           cubic feet per minute
CPU          colony forming unit
cm           centimeter
dso           median diameter (of particle)
DQO         data quality objective
EPA          U.S. Environmental Protection Agency
ETV          Environmental Technology Verification
F             Fahrenheit
fpm           feet per minute
HS           homeland security
in.            inch(es)
KC1           potassium chloride
kPa           kilopascal(s)
L             liter(s)
MERV        minimum efficiency reporting value
m             meter(s)
mm           millimeter(s)
mL           milliliter(s)
min           minute(s)
//m           micrometer(s)
NAFA        National Air Filtration Association
nm           nanometer(s)
OPC          optical particle counter
QA           quality assurance
QC           quality control
Pa            pascal(s)
PFU          plaque forming units
psig           pounds per square inch gauge
RTI           Research Triangle Institute
SAE          Society of Automotive Engineers
SMPS        scanning mobility particle sizer
                                           IV

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                                  Acknowledgments

The authors acknowledge the support of all of those who helped plan and conduct the
verification activities. In particular, we would like to thank Ted Brna, EPA's Project Manager,
and Paul Groff, EPA's Quality Assurance Manager, both of EPA's National Risk Management
Research Laboratory in Research Triangle Park, NC. We would also like to acknowledge the
assistance and participation of our stakeholder group for their input, as well as Al Veeck and the
National Air Filtration Association (NAFA), and Intertek ETL SEMKO, especially Theresa
Peck, for their help in acquiring the filters, and Aeolus Corporation for donating the filters to be
tested.

For more information on the Aeolus Corporation Synthetic Minipleat V-Cell, SMV-M13-2424
filter, contact

Bill Matkins
Aeolus Corp.
6306AOld421Rd.
Liberty, NC 27298
Phone 336-622-5122
Email billmatkins@msn.com

For more information on RTFs ETV program, contact

Debbie Franke
Research Triangle Institute
PO Box 12194
Research Triangle Park, NC 27709-2194
Telephone: (919) 541-6826
Email: dlf@rti.org

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1.0    Introduction
EPA's National Risk Management Research Laboratory contracted with the Research Triangle
Institute (RTI) to establish a homeland-security related ETV Program for products that clean
ventilation air. RTI convened a group of stakeholders representing government and industry
with knowledge and interest in the areas of homeland security and building ventilation.  The
group met in December 2002 and recommended technologies to be tested. RTI then developed
(and EPA approved) the "Test/Quality Assurance Plan for Biological Testing of General
Ventilation Filters1."  The first round of tests included ten types of filters.  The tests described in
this report were conducted following this plan.

2.0    Product Description
As shown in Figure 1, Aeolus Corporation Synthetic
Minipleat V-Cell, SMV-M13-2424 media air filter is a
V-cell filter with nominal dimensions of 0.61 by 0.61 by
0.31 m (24 by 24 by 12 in.). The filter has a plastic
frame, and the filter media color is white and red stripes.
The media is polypropylene. There are four V-cells with
minipleated media. The Aeolus Corporation part
number is SMV-M13-2424.

3.0    Test Procedure
The test program measured the culturable bioaerosol
removal efficiency of general ventilation filters.  Three
tests were required to accomplish this goal.  First,
the American Society of Heating, Refrigerating
and Air-Conditioning Engineers, Inc. (ASHRAE)
Standard 52.22 test was performed on one filter of
the test filter type to determine the minimum
efficiency reporting value (MERV) of the filter.  After determining the MERV, the biological
test using three different bioaerosols and an inert aerosol test on both clean and fully dust-loaded
filters were performed on a second filter. All tests were at an air flow rate of 0.93 m3/sec (1970
cfm) to conform to the conditions described in ASHRAE Standard 52.2.

All testing was performed in a test duct as specified in ASHRAE Standard 52.2. A schematic of
the test duct is shown in Figure 2. The test section of the duct is 610 mm (24 in.) by 610 mm (24
in.) square.  The locations of the major components, including the sampling probes, device
section (filter holder), and the aerosol generator (site of aerosol injection) are shown.

The inert testing and the ASHRAE Standard 52.2 test were performed using a potassium chloride
(KC1) aerosol.  The filters were loaded using ASHRAE dust, composed of 72% Society of
Automotive Engineers (SAE) fine, 23% powdered carbon, and 5% cotton linters. The final
pressure drop was determined by the Standard's requirements.
Figure 1. Photograph of the Aeolus Corporation
Synthetic Minipleat V-Cell, SMV-M13-2424
Media Filter

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   Room
    Air
  Blower
                  Exhaust
                    to
                   Room
Outlet Filter Bank
                 ASME
                 Nozzle
                    I.
      i            1
    ^n^f           _
Biological
Sampling
               Downstream Mixer
            X
£

i © ' ^
f f \
Fr5;
&
^
Inlet Filter / Upstream ^
Bank / Mivor i&

|
| Device Backup
i Section Filter
1 Holder
a (Used When
' "— ' Dust-loading)
Aerosol Bioloqical
Generator Sampling
           6
            Flow Control
               Valve
          Figure 2.  Schematic of Test Duct. Filter is placed in device section.
The bioaerosol tests were conducted using three microorganisms,  two bacteria and one bacterial
virus.  The spore form of the bacteria Bacillus atrophaeus  (formerly B. subtilis var niger and
Bacillus globigii or BG) was used as the simulant for gram-positive spore-forming bacteria.  The
BG spore is elliptically shaped with dimensions of 0.7 to 0.8 by 1 to 1.5 //m.  Serratia
marcescens was used as the surrogate for rod-shaped gram-negative bacteria. S. marcescens is
0.5 to 0.8 by 0.9 to 2.0//m.

The bacterial virus (bacteriophage) MS2 (0.02 to 0.03 //m), having approximately the same
aerosol characteristics as a human virus, was used as a surrogate for the viruses of similar and
larger size and shape. Although the individual virus particles are in the submicrometer size
range, the test particle size planned for the virus tests will span a range of sizes (polydispersed
bioaerosol). This test was not designed to study the removal efficiencies for single individual
virus particles; rather, it was designed to determine the removal efficiencies for virus particles as
they are commonly found indoors. A representative challenge would be a micrometer-sized,
polydispersed aerosol containing the phage because:
•   The aerosols created from sneezing and coughing vary in size from < 1 to > 20 //m, but the
    largest particles settle out and only the smaller sizes remain in the air for extended periods for
    potential removal by  an air cleaner;
•   Few viruses have been found associated with particles less than 1  //m; and
•   Nearly all 1 to 2 //m particles are deposited in the respiratory tract, while  larger particles may
    not be respired.

Bacteria suspension preparation for the aerosolization process required that the specific test
organism be grown in the laboratory and the suspension prepared for aerosol  generation in the
test rig. The microbial challenge suspensions were prepared by inoculating the test organism on
solid or liquid media, incubating the culture until mature, wiping organisms from the surface of
the pure culture (if solid media), and eluting them into sterile diluent to a known concentration.

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The bacterial virus challenge was prepared by inoculating a logarithmic phase broth culture of
the host bacteria with phage and allowing it to multiply until the majority of the host bacteria
were lysed.  The mixture was centrifuged to remove the majority of the cell fragments. The
resultant supernatant was the phage stock and was used as the challenge aerosol. The
concentration of the phage stock was approximately 1 x 109 or higher plaque forming units per
milliliter, (PFU) /mL.

The challenge organism suspensions were aerosolized using a Collison nebulizer (BGI,
Waltham, MA) at 103.4 kPa (15 psig) air pressure. The nebulizer generates droplets with an
approximate volume mean diameter of 2 //m.  The particle diameter after the water evaporates
depends on the  solids content of the suspension. Particle size was determined by the size of the
suspended organism (if singlets).

Upstream and downstream sampling of the bacteria was accomplished using a one-stage
Andersen viable bioaerosol sampler. The one-stage Andersen sampler is a 400-hole multiple-jet
impactor operating at 28 L/min.  The dso is 0.65 //m. After sampling, the petri dishes were
removed from the sampler and incubated at appropriate times and temperatures for the test
organism being used. Colony forming units (CPUs) were then enumerated and their identity
confirmed.

The microbial viruses were collected in AGI-30s.  The AGI-30 is a high velocity liquid impinger
operating at a flow rate of 12.3 to 12.6 L/min. The dso is approximately 0.3 //m.  The AGI-30 is
the sampler against which the other commonly used bioaerosol samplers are often compared.

For the inert aerosol filtration efficiency measurements, the particle sizing measurements were
made with two particle counting instruments: a Climet model 500 spectrometer/optical particle
counter (OPC) covering the particle diameter size range from 0.3 to 10 //m in 12 particle sizing
channels and a TSI scanning  mobility particle sizer (SMPS) to cover the range from 0.03 (or as
low as 16 nm) to 0.5 //m.  In  the test/QA plan there is a data control parameter for the SMPS that
states the standard deviation on upstream counts be computed for each efficiency test based on
the upstream particle counts and be less than 0.30 before the data is used. The lower size ranges
for the SMPS are included only if they meet the data control parameter. (Table A2 of test/QA
plan).

Quality Control (QC) procedures for running the test duct and the measuring equipment are
defined in the test/QA plan.

The product tested was collected by the Intertek ETL SEMKO on July 14, 2003 following the
NAFA Product Certification Program Procedural Guide3. RTI provided oversight into the
selection of representative filters. For each filter type,  a box or a minimum of four filters were
procured and sent to RTI. The filters were used as shown in Table 1.

Full details of the test method can be found in RTFs test/QA plan1.

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                    Table 1. Numbers of Filters and Expected Utilization
Tests
ASHRAE Standard 52.22test
Initial efficiency for an inert aerosol
Initial efficiency for three bioaerosols
Dust load to final pressure drop with ASHRAE dust
Efficiency for inert aerosol after dust-loading
Efficiency for three bioaerosols after dust-loading
Reserve filter*
Filter #
1
X






2

X
X
X
X
X

3






X
4






X
       *Filters # 3 and # 4 have been kept in reserve to be used if needed.

4.0    Test Results
The bioaerosol filtration efficiency results are found in Table 2.

                     Table 2. Bioaerosol Filtration Results for Filter # 2
Filter
Condition
Clean
Dust-loaded
Pressure
Drop
Pa (in. H2O)
77(0.31)
348(1.40)
Filtration
Efficiency for
Removal of
B. atrophaeus, %
69
99
Filtration
Efficiency for
Removal of
S. marcescens, %
64
99.5
Filtration
Efficiency for
Removal of
MS2 phage, %
73
99
The ASHRAE filtration efficiencies and the MERV are shown in Table 3.  The filtration
efficiencies (average of the minimum composite efficiency) are presented by particle size
groupings: El, 0.3 to 1.0 //m; E2, 1.0 to 3.0 //m; and E3, 3.0 //m to 10 //m.. The full ASHRAE
52.2 test results are provided in the Appendix.

       Table 3. Summary of Removal Efficiency Using ASHRAE 52.2 Test for Filter # 1
Filter
Aeolus Corporation
Synthetic Minipleat V-Cell,
SMV-M13-2424
El
0.3 to 1.0 Aim,
57
E2
1.0 to 3.0 Aim,
85
E3
3.0 to 10 Aim,
97
MERV
12 at 0.93m3/sec
(1970 cfm)
The filtration efficiency for inert particles is plotted so that the efficiencies for particles from
about 0.03 to 10 //m can be observed (Figure 3). Note that this is a logarithmic base 10 scale on

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the X axis.  Two instruments were used to obtain the measurements. The SMPS was used to
measure particles up to 0.5 //m and the OPC was used for particles from 0.3 to 10 //m. There is
good agreement in the size range covered by both instruments. These measurements were made
on a filter when clean and then when dust-loaded.


80


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it
^ 40-
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1.00
. '


















•


n

SMPS


_

OPC

A


A
OPC: loaded


10.00
                               Particle Diameter (|jm)


      Figure 3. Summary of the Inert Aerosol Filtration Efficiency Data for the Clean
      and Dust-Loaded Filter, # 2.
The quality assurance officer has reviewed the test results and the quality control data and has
concluded that the data quality objectives (DQOs) (Table 4) given in the approved test/QA plan
have been attained.

  Table 4. DQOs for Precision of Filtration Efficiency Measurements for Culturable Bioaerosol
Data quality objective
Precision of filtration
efficiency, %
Test organism
Spore-forming bacteria
(B. atrophaeus)
±8a
Vegetative bacteria
(S. mar ce seem)
±lla
Bacterial virus
(MS2 phage)
±13a
a Based on +/- one standard deviation of penetration computed from the coefficient of variance
upstream and downstream culturable counts.

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 5.0    Limitations and Applications

 This verification report addresses two performance measures of media air filters: filtration
 efficiency and pressure drop. Users may wish to consider other performance parameters such as
 service life and cost when selecting a general ventilation air filter for their application.

 In accordance with the test/QA plan1, this verification statement is applicable to filters
 manufactured from December 2003 through November 2006.

6.0 References

 1.  RTI. 2003. Test/QA Plan for Biological Testing of General Ventilation Filters. Research
    Triangle Institute, Research Triangle Park, NC.

 2.  ANSI/ASHRAE Standard 52.2-1999, Method of Testing General Ventilation Air-Cleaning
    Devices,  American National Standards Institute/American Society of Heating, Refrigerating
    and Air-Conditioning Engineers, Atlanta, GA.

 3.  NAF A (National Air Filtration Association). 2001. Product Certification Program
    Procedural Guide Approved Version 1, Second Revision, February 2001. Virginia Beach,
    VA.

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        Appendix ASHRAE 52.2 Test Report

For Aeolus Corporation Synthetic Minipleat V-Cell, SMV-M13-2424
ASHRAE 52.2 TEST REPORT

Manufacturer:    Aeolus Corp.
Product Name:    Synthetic Minipleat V-Cell
                  SMV-M13-2424
ETV Filter ID:     AEO3-A

   RTI Report No.  AY09050301
         Test Laboratory:
               RTI
           919-541-6941
                   7

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                                                                                   Page 1 of 3
                 ASHRAE Std. 52.2 Air Cleaner Performance Report Summary
                                This report applies to the tested device only.
Laboratory Data
RTI Report No.
Test Laboratory
Operator
Particle Counter(s):

Device Manufacturer's Data
Manufacturer
Product Name
Product Model
Test requested by
Sample obtained from
Catalog  rating:
Specified test conditions:
Device Description
Nominal Dimensions (in.):
Generic name
Amount and type of adhesive
Other attributes

Test Conditions
Airflow (cfm)
Face Velocity (fpm)
Test aerosol type:
Remarks

Resistance Test Results
Initial resistance (in. wg)
                                AY09050301
                    Date
           9/5/03
                                Research Triangle Institute
                                Clayton
                                Brand
Climet
Supervisor  Owen/Hanley
Model
     500
                                Aeolus Corp.
                                Synthetic Minipleat V-Cell SMV-M13-2424
                                EPA
                                NAFA
                                Airflow rate
                                Airflow (cfm)
                                Face Velocity (fpm)
                                24x24x12
             NA
             1970
      Initial dP (in. wg)
      Final dP (in. wg)
            NA
            1.40
                                                         493
          (height x width x depth)
                                V-Cell
                     Media color    white/red
                                NA
                                minipleated media in four Vs
                                   1970
   Temperature (F)
    73
                                    493
   Final Pressure Drop (in. wg)
RH (%)
 1.40
51
                                    KG I
                                   0.31
          Final resistance (in. wg)
                  1.40
                                               57
Minimum Efficiency Reporting Data
Composite average efficiencies             E1	
Air cleaner average Arrestance per Std 52.1:
Minimum efficiency reporting value (MERV) for the device:
                 E2
    85
     E3
97
                                                         NA
                                                                        12
                                      1970    cfm
                                                   8

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                        Report No.  AY09050301
                        Research Triangle Institute
                                                             Initial Efficiency


                                                             After 1st loading


                                                             After 2nd loading


                                                             After 3rd loading


                                                             After 4th loading


                                                             After 5th loading
              0.1                    1                    10
                     Particle Diameter (micrometers)
        Minimum Composite Curve
 0.1                1                1C
      Particle Diameter (micrometers)
   Resistance to Airflow
       for clean filter
500    1000   1500    2000
     Air Flow (cfm)
                           2500
Figure A-1.  Filtration Efficiency and Flow Resistance Curves for
            Aeolus Synthetic Minipleat V-cell SMV-M14-2424 Filter.

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                                             TABULATED DATA SUMMARY
                                             Report No. AY09050301
                                             Research Triangle Institute
Summary of Test Conditions:
Product Manufacturer
Product Name
Nominal Dimensions (in.)
Airflow (cfm)
Final Resistance (in.  H2O)
OPC Channel Number
Min. Diam. (urn)
Max. Diam. (urn)
Geo. Mean Diam. (urn)
Initial efficiency
after first dust load
after second dust load
after third dust load
after fourth dust load
after fifth dust load
Minimum Composite Efficiency
                     Aeolus Corp.
                     Synthetic Minipleat V-Cell SMV-M13-2424
                     24x24x 12
                       1970
                        1.40
                                                   Efficiency (%) per Indicated Size Range
                          1
                         0.3
                         0.4
                       2
                     0.4
                    0.55
     3
  0.55
   0.7
  4
0.7
  1
 5
 1
1.3
 6
1.3
1.6
  7
1.6
2.2
  8
2.2
  3
 9
 3
 4
 10
  4
5.5
 11
5.5
  7
                       0.35   0.47  0.62   0.84
                         48
                      53
    58
68
77
83
 89
 93
96
 97
 98
12
 7
10
                                         1.14   1.44   1.88   2.57   3.46   4.69   6.20   8.37
Run No.
AY09040302
AY09050301
AY09050302
AY09050303
AY09050304
AY09060301

48
56
76
86
92
94

53
62
81
90
94
96

58
68
86
93
96
98

68
76
92
97
98
99

77
85
96
99
99
99

83
90
98
99
100
100

89
93
99
100
100
100

93
96
99
100
100
100

96
97
100
100
100
100

97
98
100
100
100
100

98
99
100
100
100
100

99
98
100
100
100
100
98
E1 =
E2 =
E3 =

MERV
57
85
97

12
(E1 is the average of the minimum composite efficiency values for particle diameters from 0.3 to 1 urn.)
(E2 is the average of the minimum composite efficiency values for particle diameters from 1 to 3 urn.)
(E3 is the average of the minimum composite efficiency values for particle diameters from 3 to 10 urn.)
Resistance to Airflow for Clean Filter:
Airflow
    50
    75
   100
   125
   Airflow
    (m3/s)

    0.465
    0.697
    0.930
    1.162
            Airflow
             (cfm)

              985
             1478
             1970
             2463
Air Velocity
 (fpm)

   246
   369
   493
   616
Resistance to Airflow with Loading at 0.93 m3/s (1970 cfm)
Initial
After first dust load
After second dust load
After third dust load
After fourth dust load
After fifth dust load
                     Resistance
                      (in. H20)

                       0.31
                       0.35
                       0.58
                       0.85
                       1.13
                       1.40
                         Resistance
                           (Pa)

                            77
                            86
                           145
                           213
                           280
                           348
   Air Velocity
      (m/s)

     1.251
     1.876
     2.502
     3.127
          Resistance
          (in. H20)

            0.12
            0.21
            0.31
            0.45
                Resistance
                  (Pa)

                    30
                    52
                    77
                   112
Weight gain of filter after completion of dust loading steps
                                                  252.9 g
                                                     10

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