United States
                Environmental Protection
                Agency	
                       Pesticides And
                       Toxic Substances
                       (7508W)	
738-F-91-104
March 1991
                R.E.D.   FACTS
     Pesticide
Reregistration
                Methoprene
     All pesticides sold or used in the United States must be registered by
EPA, based on scientific studies showing that they can be used without
posing unreasonable risks to people or the environment. Because of
advances in scientific knowledge, the law requires that pesticides which
were first registered years ago be reregistered to ensure that they meet
today's more stringent standards.

     In evaluating pesticides for reregistration, EPA obtains from pesticide
producers and reviews a complete set  of studies showing the human health
and environmental effects of each  pesticide. The Agency imposes any
regulatory controls that are needed to  effectively manage each pesticide's
risks.  EPA then reregisters pesticides that can be used without posing
undue hazards to human health or the environment.
  Methoprene
     When a pesticide is eligible for reregistration, EPA announces this and
explains why in a Reregistration Eligibility Document, or RED. This fact
sheet summarizes the information in the RED for methoprene.

     Methoprene, which is sold under the trade name Altosid, is an insect
growth regulator. It is considered a biochemical pesticide because rather
than controlling target pests through direct toxicity, methoprene interferes
with an insect's life cycle and prevents it from reaching maturity or
reproducing. Methoprene is used in the production of a number of foods
including meat, milk, eggs, mushrooms, peanuts, rice and cereals. It is also
used in aquatic areas to control mosquitoes and several types of flies, moths,
beetles and fleas. All pesticide products that contain methoprene as the sole
active ingredient are eligible for reregistration except the briquette  or slow-
release formulation.
   Regulatory        Methoprene was first registered by EPA as a conventional, chemical
       History   pesticide in 1975. EPA issued a Registration Standard for methoprene in
                February 1982. Subsequently, the Agency reclassified methoprene as a
                biochemical pesticide.

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       Health        Methoprene is a biochemical pesticide, and its health-related data base
      Effects   consists mainly of a group of screening studies designed to show its toxicity
                 and developmental effects in people and other nontarget organisms.  If these
                 studies indicated potential adverse effects, further studies  on environmental
                 fate, ecological effects and food residues would have been required.

                      The results of these screening tests and other  available studies on
                 methoprene indicate that it is of low toxicity and poses  little risk to people
                 and other nontarget species, with one  exception.  Methoprene is highly
                 acutely toxic to estuarine invertebrates.  Use of the  briquette or slow-release
                 formulation of methoprene in aquatic environments could pose an undue risk
                 to these species. Further study of this formulation  is being required, as
                 described further below.
                      Methoprene showed no significant adverse toxicological effects in any
                 human health effects screening studies. The pesticide has very low acute
                 oral and inhalation toxicity potential, and is not an eye or skin irritant (it has
                 been placed in toxicity category IV,  the least toxic category, for these
                 effects).  It also is not a human skin sensitizer.  Methoprene is of low acute
                 dermal toxicity (it has been placed in toxicity category III).

                      In subchronic studies, methoprene showed some evidence of causing
                 increased liver weights in test animals, at high dose levels. However, in
                 chronic effects and oncogenicity studies, no effects were observed even at
                 the highest dose levels.  Other studies show that methoprene does not cause
                 developmental or reproductive  effects, is not mutagenic, and metabolizes
                 rapidly and completely in mammals.

      Routes   Through the Diet
Of Exposure        People may be exposed to small amounts of methoprene through the
                 food supply.  However, the amount of methoprene in the U.S. consumer's
                 diet is well below the level at which any adverse health effects could occur.

                      Tolerances, or legal residue limits, have been established for residues
                 of methoprene in or on a number of raw agricultural  commodities (also see
                 40 CFR 180.359).   Several international Codex Maximum Residue Levels
                 (MRLs) and Canadian tolerances also have been established, as listed below:

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                        Methoprene Tolerances
Canadian
Commodity
  U.S.     Codex

Tolerance  MRL    Tolerance
  (ppm)    (ppm)
  (ppm)
Cattle, fat
Cattle, meat
Cattle, meat byproducts
Eggs
Goats, fat
Goats, meat
Goats, meat byproducts
Hogs, fat
Hogs, meat
Hogs, meat byproducts
Horses, fat
Horses, meat
Horses, meat byproducts
Milk
Mushrooms
Peanuts
Peanut hulls
Poultry, fat
Poultry, meat
Poultry, meat byproducts
Sheep, fat
Sheep, meat
Sheep, meat byproducts
Meat, fat
Cattle milk
Edible offal (mammalian)
0.3
0.1
0.1
0.05
0.3
0.1
0.1
0.3
0.1
0.1
0.3
0.1
0.1
0.05
1.0
2.0
40.00
0.5
0.5
0.5
0.3
0.1
0.1






0.05










0.2
2.0







0.1
0.05
0.1
                    0.1

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                    EPA has reassessed the existing tolerances and finds that they are set at
                   appropriate levels. Although the U.S. tolerances for mushrooms and meat
                   fat are higher than the Codex and Canadian limits, EPA is not lowering the
                   U.S. tolerances at this time.  These tolerances are set at levels that
                   accommodate current methoprene use practices in this country.  No changes
                   are needed to adequately protect the public health.

                    No new tolerances are required to cover the existing methoprene uses.
                   (Please note that a petition is pending to establish additional methoprene
                   tolerances for cereal grains, grain milled fractions and rice hulls.)

                   During Application
                    People  can be exposed to methoprene while mixing, loading or applying the
                   pesticide, and while working among treated crops.  However, since
                   methoprene is of such low acute toxicity, and poses no risk of oncogenic,
                   reproductive, developmental or neurotoxic effects, EPA is satisfied that
                   methoprene poses no risks to people who  are occupationally exposed to the
                   pesticide.

Environmental    All the environmental fate  data requirements for methoprene are satisfied.
       Hazards   The information available to EPA indicates that methoprene will not result
                   in unreasonable adverse effects to the environment.  However, the
                   ecological effects studies on methoprene suggest that use of the briquette or
                   slow-release formulation in estuarine areas may cause undue risks to
                   estuarine invertebrates, since the pesticide  is highly acutely toxic to these
                   organisms. EPA is therefore  requiring further study of this methoprene
                   use.

                   Environmental Fate
                    Methoprene degrades rapidly in sunlight,  both in water and on inert
                   surfaces. The pesticide  also is metabolized rapidly in soil and does not
                   leach.  Thus, it should not persist in soil or contaminate ground water.

                   Ecological Effects
                    Methoprene has been shown to be practically non-toxic to mallard ducks,
                   and had no effect on quail reproduction. However, the pesticide  is
                   moderately toxic to warm water, freshwater fish, and is slightly toxic to
                   cold water, freshwater fish.

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                        Methoprene is very highly toxic to freshwater invertebrates, as seen in
                       studies with crayfish and Daphnia magna. The pesticide also can be very
                       highly acutely toxic to estuarine and marine invertebrates,  as seen in studies
                       with grass shrimp and mud-crabs.  Marine organisms are not likely to be
                       exposed to methoprene, but estuarine organisms are likely to be exposed as
                       a result of the use of methoprene as a mosquito larvicide.
   Additional Data
           Required
  Product Labeling
Changes Required
 Methoprene degrades rapidly in water so the use of most formulations in
estuaries is not of concern. However, the slow-release briquette
formulation is of concern to EPA because it causes estuarine organisms to
be exposed to methoprene over an extended period of time.  An estuarine
invertebrate life cycle toxicity study is being required to adequately
characterize the chronic toxicity of methoprene to estuarine organisms.

 EPA has a sufficient battery of studies to support the reregistration of most
uses of methoprene.  The only use of concern is the aquatic, mosquito
larvicide use involving the briquette formulation.  An estuarine invertebrate
life cycle study is being required to determine whether long term exposure
of these species to methoprene through the briquette formulation  poses
adverse effects. In addition, an octanol/water partition coefficient study is
being required to  complete the product chemistry data base for methoprene.

 End-use products containing  methoprene must bear a statement warning the
applicator that improper use could harm aquatic invertebrates.  These
products also must bear an updated water contamination warning. Please
see the Reregistration Eligibility  Document for a detailed list of labeling
requirements.
        Regulatory    * The studies available to EPA indicate that the biochemical insect growth
        Conclusion   regulator methoprene is of low toxicity and poses very little hazard to
                       people and most other nontarget species.
                        * Methoprene is highly acutely toxic to estuarine invertebrates, however,
                       and these organisms may be exposed to sufficient amounts of methoprene
                       through its aquatic, slow-release, briquette formulation to experience
                       adverse effects.  An estuarine invertebrate life cycle toxicity study must be
                       completed before the briquette formulation of methoprene will be eligible
                       for reregistration.

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                * All other registered methoprene products can be used without causing
                unreasonable adverse effects in people or the environment. Therefore, all
                pesticide products containing methoprene as the sole active ingredient,
                except the briquette formulation, are eligible for reregistration.
                * EPA will reregister individual products containing methoprene once
                product specific data and revised product labeling are submitted to and
                accepted by the Agency.
   For More   EPA requests public comments on the Reregistration Eligibility Document
Information   for methoprene, and will consider comments received during the next
                several months.  To obtain a copy of the RED, or to submit written
                comments, please contact the Public Response and Program Resources
                Branch, Field Operations Division (7506C), Office of Pesticide Programs,
                U.S. EPA, Washington, D.C. 20460.  Call 703-557-4436,  or FAX to 703-
                557-1884.

                To obtain a copy of the Registration Standard for methoprene,  please
                contact the National Technical Information Service (NTIS), 5285 Port Royal
                Road, Springfield, VA. 22161.  Call 703-487-4650, and request document
                #PB87-109443.

                For more information about methoprene or about EPA's pesticide
                reregistration program, please contact the Special Review and Reregistration
                Division (7508W),  Office of Pesticide Programs, U.S. EPA, Washington,
                D.C. 20460.  Call 703-808-8000, or FAX your request to 703-308-8005.

                For information about the health effects of pesticides, or for assistance in
                recognizing and managing pesticide poisoning symptoms, please contact the
                National Pesticides  Telecommunications Network (NPTN). Call toll-free 1-
                800-858-7378, 24 hours  a day, seven days a week, or FAX  your inquiry to
                806-743-3094.

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