Jflk ^™^% Jl United States
           Environmental Protection                         EPA/600/R-09/057
           Agency                                       Ju|y 2Q09
               External Review Draft

          Nanomaterial Case Studies:
    Nanoscale Titanium Dioxide in Water
    Treatment and  in Topical Sunscreen
                          NOTICE


This draft document is distributed solely for the purpose of public comment and peer input under
applicable information quality guidelines. It has not been formally disseminated by EPA. It does
not represent and should not be construed to represent any Agency determination or policy.
             National Center for Environmental Assessment
                Office of Research and Development
                U.S. Environmental Protection Agency
                   Research Triangle Park, NC

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                                Disclaimer
This draft document is distributed solely for the purpose of public comment and peer input under
applicable information quality guidelines.  It has not been formally disseminated by EPA. It does not
represent and should not be construed to represent any Agency determination or policy. Identification of
a commercial product does not imply either endorsement or criticism of the product by EPA.

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               Table of Contents
List of Figures
List of Tables
Abbreviations
Authors, Contributors, and Reviewers
Preamble
Chapter 1. Introduction
1.1. Backaround
1 .2. How to Read this Document
1.3. Terminoloay
1.4. Conventional Ti02
1.5. Nano-Ti02
1.5.1. Water Treatment
1.5.2. Sunscreen
1.6. Analytical Methods
1.6.1. Methods for Laboratory Research
1.6.2. Methods and Instrumentation to Assess Environmental Occurrence
1.6.3. Methods and Instrumentation to Assess Workplace Exposure
Questions about Characterizina Nanoscale Titanium Dioxide
Chapter 2. Life Cycle Stages
2.1. Feedstocks
Questions about Feedstocks
2.2. Manufacturina
2.2.1. Water Treatment
2.2.2. Sunscreen
Questions about Manufacturina
2.3. Distribution and Storaae
2.3.1. Water Treatment
2.3.2. Sunscreen
Questions about Distribution and Storaae
2.4. Use
2.4.1. Water Treatment
2.4.2. Sunscreen
Questions about Use
2.5. Disposal
2.5.1. Water Treatment
2.5.2. Sunscreen
Questions about Disposal
Chapter 3. Fate and Transport
3.1. Water
3.1.1. Drinkina Water Treatment-specific
3.1.2. Sunscreen-specific
3.2. Soil
3.2.1. Drinkina Water Treatment-specific
V
vi
vii
xii
xiv
1-1
1-1
1-3
1-4
1-5
1-6
1-11
1-12
1-14
1-14
1-16
1-17
1-19
2-1
2-1
2-2
2-2
2-3
2-3
2-4
2-5
2-5
2-5
2-6
2-6
2-6
2-8
2-9
2-10
2-10
2-11
2-11
3-1
3-2
3-4
3-5
3-5
3-7
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3.2.2. Sunscreen-specific
3.3. Air
Questions about Fate and Transport
Chapter 4. Exposure-Dose Characterization
4.1 . Aaareaate Exposure to Nano-Ti02 from Multiple Sources and Pathways
4.2. Cumulative Exposure to Nano-Ti02 and Other Contaminants
4.3. Models to Estimate Exposure
4.4. Biota
4.4.1. Aquatic
4.4.2. Terrestrial
4.4.3. Food Web
4.5. Humans
4.5.1. General Population
4.5.2. Occupational
4.6. Dose
4.6.1. Respiratory (Inhalation and Instillation)
4.6.2. Dermal
4.6.3. Inaestion
4.6.4. Blood Brain Barrier and Placental Transfer
4.6.5. Dose-metrics
Questions about Exposure-dose Characterization
Chapter 5. Characterization of Effects
5.1. Factors that Influence Ecoloaical and Health Effects of Nano-Ti02
5.1.1. Nano-Ti02 Physicochemical Characteristics
5.1.2. Experimental Conditions
5.1.3. Environmental Conditions
5.1.4. Summary
5.2. Ecoloaical Effects
5.2.1. Ecoloaical Effects of Nano-Ti02 Exposure
Questions about Ecoloaical Effects
5.3. Health Effects
5.3.1. Non-Carcinoaenic Effects
5.3.2. Carcinoaenic Effects
Questions about Health Effects
References
3-7
3-8
3-9
4-1
4-2
4-3
4-6
4-6
4-7
4-8
4-9
4-9
4-10
4-13
4-15
4-16
4-18
4-25
4-26
4-28
4-30
5-1
5-1
5-2
5-6
5-9
5-11
5-11
5-12
5-29
5-30
5-30
5-64
5-74
R-1
Appendix A. Nano-TiCh in Sunscreen: Background Information	A-1




Appendix B. Nano-TiCh in Sunscreen: Manufacturing Processes	B-1




Appendix C. Nano-TiCh Exposure Control in the Workplace and Laboratory	C-1
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                                 List of Figures
Figure 1 -1. Basic structure of comprehensive environmental assessment (CEA) as a framework for identifying and
         prioritizing research efforts.	1-2
Figure 4-1. Possible pathways of nano-Ti02 skin penetration.	4-20
Figure 5-1. The pulmonary effects of fine Ti02 and nano-Ti02 exposure through inhalation or instillation.	5-72
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                                      List  of  Tables
Table 1-1.
Table 1-2.
Table 1-3.
Table 1-4.

Table 4-1.
Table 4-2.
Examples of nano-Ti02 physicochemical properties..
Characterization of three nano-Ti02 particle types. _
Analytical methods for characterizing nanomaterials in aerosol and in liquid.	
Analytical methods for nanomaterials in soil, sediment, and ground water for size fraction and distribution,
surface area, and phase and structure.	
Tissue concentrations of various pollutants in fish after exposures to nano-Ti02 in water.	
Estimated dermal exposure to nano-Ti02 from sunscreen containing 5% nano-Ti02 for adults and 3-year-
_ 1-7
 1-10
 1-15

 1-17
  4-4
Table 4-3.
Table 4-4.
Table 4-5.
Table 5-1.
Table 5-2.
Table 5-3.
Table 5-4.
Table 5-5.
Table 5-6.
Table 5-7.
old children.
Nano-Ti02 disposition in animals after inhalation or intratracheal instillation of nano-Ti02.
Overview of Ti02 skin absorption/penetration studies.
Animal studies that measured Ti concentrations in brain after nano-Ti02 exposures through injection or
oral aavaae.
Recommendations for measurina exposure durina inhalation studies.
Recommendations for off-line nanomaterial characterization for toxicoloaical studies.
Summary of nano-Ti02 ecoloaical effects.
Summary of health effects of nano-Ti02 particles in mammalian animal models: dermal route.
Summary of health effects of nano-Ti02 particles in mammalian animal models: oral route.
Summary of health effects of nano-Ti02 particles in mammalian animal models: respiratory route.
Summary of health effects of nano-Ti02 particles in mammalian animal models: other (injection, ocular)
4-12
4-17
4-21
4-26
5-5
5-6
5-13
5-33
5-37
5-44

Table 5-8.

Table 5-9.
Table 5-10.
route.	
Treatments and pulmonary tumor incidences in rats exposed to fine and nano-Ti02 through intra-tracheal
instillation in Pott and Roller (2005) study.	
Incidence of tumor in the abdominal cavity of rats intraperitoneally injected with photocatalytic nano-Ti02.
 Results of nano-Ti02 carcinogenicity studies in animals.	
 5-62

 5-67
 5-69
 5-73
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                                      Abbreviations
a-HBDH
yH2 AX
i;
n
ag
ng
ng/g
ng/kg
ng/L
(iL
(im
nm2/cm3
4-MBC
ACGIH
ACROS
AFM
Al2(S04)3-16H20
ALP
ALT
As(lll)
As(V)
AST
BAL
BALF
BAuA
BBB
BET
BrdU
BUN
BW
Ceo
Ca2+
CCOHS
CE
CEA
CK
cm2
cm3
CMD
CPC
CREM
CVD
DIN
                         Alpha-hydroxybutyrate dehydrogenase
                         Phosphorylated form of histone H2AX (phosphorylation of H2AX at serine 1 39)
                         Chi potential
                         Pi, approximately equal to 3. 14159
                         Geometric standard deviation
                         Microgram(s)
                         Microgram(s) per gram
                         Microgram(s) per kilogram
                         Microgram(s) per liter
                         Microliter(s)
                         Micrometer(s)
                         Micrometer(s) squared per centimeter cubed
                         4-methylbenzylidene camphor
                         American Conference of Governmental Industrial Hygienists
                         Acros Organics
                         Atomic force microscopy
                         Alum
                         Aluminum oxide, also known as alumina
                         Alkaline phosphatase
                         Alanine aminotransferase
                         Arsenite
                         Arsenate
                         Aspartate aminotransferase
                         Bronchoalveolar lavage
                         Bronchoalveolar lavage fluid
                         German Occupational Safety and Health (Bundesanstalt fur Arbeitsschutz und Arbeitsmedizin)
                         Blood brain barrier
                         Brunauer, Emmett, Teller method of calculating surface area
                         Bromo-deoxy-uridine
                         Blood urea nitrogen
                         Body weight
                         Fullerene
                         Calcium cation
                         Canadian Centre for Occupational Health and Safety
                         Capillary electrophoresis
                         Comprehensive environmental assessment
                         Creatinine kinase
                         Centimeter(s) squared
                         Centimeter(s) cubed
                         Count median diameter
                         Condensation particle counter
                         Council for Regulatory Environmental Modeling
                         Chemical vapor deposition
                         Deutsches Institut fiir Normung (German Institute for Standardization)
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      DLS                  Dynamic light scattering
      DMA(V)               Dimethylarsinic acid
      DMEM                Dulbecco's Modified Eagle's Medium
      DPPC                 Dipalmitoyl phosphatidylcholine
      Fr                    Estimated concentration required to induce a threshold positive response, where stimulation index
                            equals 3
      EC50                 Effective concentration 50; the concentration at which 50% of subjects show a response
      EDS                  Electron-dispersive X-ray analysis
      E-FAST V2.0           Exposure and Fate Assessment Screening Tool Version 2.0
      EHS                  Environmental health and safety
      ELISA                Enzyme-linked immunosorbent assay
      ELPI                  Electrical low pressure impactor
      EM                    Electron microscopy
      EN                    European Norm
      EPA                  U.S. Environmental Protection Agency
      ED                    European Union
      EWG                 Environmental Working Group
      F344                  Fischer 344
      FDA                  U.S. Food and Drug Administration
      FE-SEM               Field emission-type scanning electron microscopy
      FeTiOs                llmenite
      FFF                   Field flow fractionation
      FHD                  Flame hydrolysis deposition
      FIFFF                 Flow field flow fraction  (also known as flow FFF)
      g                     Gram(s)
      g/kg                  Gram(s) per kilogram
      GFAP                 Glial fibrillary acidic protein
      GGT                  y-Glutamyltransferase
      GSD                  Geometric standard deviation
      GSH                  Reduced glutathione
      GSH-Px               Glutathione peroxidase
      GST                  Glutathione-S-transferase
      H202                  Hydrogen peroxide
      H2S04                Sulfuric acid
      HBSS                 Hank's Basic Salt Solution
      HCI                   Hydrochloric acid
      HEPA                 High efficiency particulate air
      HPLC                 High performance liquid chromatography
      hprt                  Hypoxanthine-guanine phosphoribosyltransferase (gene)
      HRTEM               High resolution transmission electron microscopy
      Hz                    Hertz
      i.p.                    Intraperitoneal
      i.v.                    Intravenous
      IAEA                 International Atomic Energy Agency
      IARC                 International Agency for Research on Cancer
      IC2o, \Cis               Inhibitory concentration at which organisms show 20%, 25% inhibition in measured endpoints
      ICP                   Inductively coupled plasma
      ICP-AES               Inductively coupled plasma atomic emission spectrometry
      ICP-MS               Inductively coupled plasma-mass spectrometry
      IEP                   Isoelectric point
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      IFN-y
      IL-10
      IL-1P
      IL-4
      IL-6
      IL-8 (KC)
      ILSI
      IOAA
      ISO
      ITT
      K+
      kg
      L
      LCso
      LDH
      LIBD
      LOEC
      LOEL
      IPS
      m2
      m2/g
      m3
      MARA
      MCL
      mg
      mg/cm2
      mg/kg
      mg/L
      mg/m3
      mg/mL
      Mg2+
      MgCI2
      micro-Ti02
      mL/kg/day
      mm
      mM
      MMA(V)
      MMAD
      MPPS
      mSv
      MTC
      MTP
      Na+
      NaCI
      NAG
      Nano-TiCh
      Nano-Ti02F-1R
      NCEA
      Nano-Ti02
      ng/mL
Interferon-gamma
lnterleukin-10
lnterleukin-1(3
lnterleukin-4
lnterleukin-6
IL-8 = interleukin-8, KC = chemokine (CXC motif) ligand 1 (CXCL1)
International Life Sciences Institute
(U.S. EPA) Immediate Office of the Assistant Administrator
International Organization for Standardization
Isopropyl titanium triisostearate
Potassium cation
Kilogram(s)
Liter(s)
Lethal concentration 50; the concentration at which 50% of subjects died
Lactate dehydrogenase
Laser-induced breakdown detection
Lowest observed effect concentration
Lowest observed effect level
Lipopolysaccharide
Meter(s) squared
Meter(s) squared per gram
Meter(s) cubed
Microbial array for risk assessment (assay)
Maximum contaminant level
Milligram(s)
Milligram(s) per centimeter squared
Milligram(s) per kilogram
Milligram(s) per liter
Milligram(s) per meter cubed
Milligram(s) per milliliter
Magnesium cation
Magnesium chloride
Microscale titanium dioxide
Milliliter(s) per kilogram per day
Millimeter(s)
Millimolar
Monomethylarsonic acid
Mass median aerodynamic diameter
Maximum penetrating particle size
Milliseviert
Microbial Toxic Concentration, in microbial array for risk assessment (MARA) assay
Microsomal triglyceride
Sodium cation
Sodium chloride
Nacetyl-p-glucosaminidase
Nanoscale titanium dioxide
Nanoscale titanium dioxide a formula containing nano-Ti02 that is 3%anatase and 97%rutile
(U.S. EPA) National Center for Exposure Assessment
Nanoscale titanium dioxide
Nanogram(s) per milliliter
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      NHEERL             (U.S. EPA) National Health and Environmental Research Laboratory
      NIOSH               National Institute for Occupational Safety and Health
      nm                  Nanometer(s)
      NMR                 Nuclear magnetic resonance
      NMRI                Naval Medical Research Institute
      NOEC                No observed effect concentration
      NOSH                Nanoparticle Occupational Safety and Health (Consortium)
      02"                  Superoxide radical anion
      OC                  Octocrylene
      °C                   Degree(s) Celsius
      OECD                Organization for Economic Co-operation and Development
      OH                  Hydroxyl
      • OH                 Hydroxyl radical(s)
      • OOH                Hydroperoxl radical(s)
      OM                  Octyl methoxycinnamate
      OPPT                (U.S. EPA) Office of Pollution Prevention and Toxics
      OPT                 Optical particle counter
      ORD                 (U.S. EPA) Office of Research and Development
      ORISE               Oak Ridge Institute for Science and Education
      OSHA PEL           Occupational Safety and Health Administration permissible exposure limit
      OSP                 (U. S. EPA) Office of Science Policy
      p                    Pink-eyed dilution
      P25                  AEROXIDE® P25
      PAM                 Pulse amplitude modulation
      PBS                 Phosphate buffered saline
      PEC                 Predicted environmental concentration
      pH                  Measure of acidity or alkalinity of a solution
      pHpzc                pH at the point of zero charge
      PIGF                 Placenta growth factor
      PMN                 Polymorphonuclear neutrophil
      PN EC                Predicted no-effect concentration
      PPE                 Personal protective equipment
      ppm                 Part(s) per million
      PTFE                Polytetrafluoroethylene
      Pt                   Platinum
      PTM                 Particle tracking model
      ffn                  Pink-eyed unstable
      RLE-TN              Rat alveolar type  11 epithelial cell line
      ROS                 Reactive oxygen species
      /PTM                Radius particle tracking model
      RT-PCR              Reverse transcription polymerase chain reaction
      s.c.                  Subcutaneous
      SAXS/WAXS          Small- and wide-angle X-ray scattering
      SCCNFP             Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers
      SCCP                Scientific Committee on Consumer Products
      SCID                 Severe combined immunodeficiency
      SEC                 Size exclusion chromatography
      SEM                 Scanning electron microscopy
      Si02                 Silicon dioxide
      SMPS                Scanning mobility particle sizer
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      SOD
      SPF
      SPM
      St-Cn
      SWCNT
      TEC
      TEM
      TEOM*
      IFF
      TGA
      TGF-p
      THF
      Ti
      TiCU
      Ti02
      TiOS04
      TLV
      TNF-a
      TRAIL
      TS
      TUNEL
      U.S. EPA
      USP
      UV
      UV-A
      UV-B
      VEDIC
      WHMIS
      Wt%
      XAS
      XPS
      XRD
      ZnO
Superoxide dismutase
Sunburn protection factor
Scanning probe microscopy
Sunscreen standard C from the Japan Cosmetic Industry
Single-walled carbon nanotube(s)
Threshold effect concentration
Transmission electron microscopy
Tampered element oscillating microbalance
Tangential-flow ultrafiltration
Australian Therapeutic Goods Administration
Transforming growth factor-beta
Tetrahydrofuran
Titanium
Titanium tetrachloride
Titanium dioxide
Titanyl sulfate
Threshold limit value
Tumor necrosis factor-alpha
Tumor necrosis factor-related apoptosis-inducing ligand
Technical Specification
Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling
U.S. Environmental Protection Agency
U.S. Pharmacopeia
Ultraviolet (light/radiation), wavelengths in the range of 10-400 nm
Ultraviolet A, wavelengths in the range of 320-400 nm
Ultraviolet B, wavelengths in the range of 290-320 nm
Video-enhanced differential interference contrast
Workplace Hazardous Materials Information System
Weight percent
X-ray absorption spectroscopy
X-ray photon spectroscopy
X-ray diffraction
Zinc oxide
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     Authors, Contributors,  and Reviewers

Principal Authors
J. Michael Davis (Project Leader), NCEA, ORD, U.S. EPA
Amy Wang, ORISE Postdoctoral Fellow with NCEA, ORD, U.S. EPA
Jo Anne Shatkin, The Cadmus Group (currently with CLF Ventures)

Co-Authors
Judith A. Graham, Private Consultant
Maureen Gwinn, NCEA, ORD, U.S. EPA
Brent Ranalli, The Cadmus Group

Contributors
Christian Andersen, NHEERL, ORD, U.S. EPA
Walter Cybulski, OSP, ORD, U.S. EPA
J. Allen Davis, NCEA, ORD, U.S. EPA
Steve Diamond, NHEERL, ORD, U.S. EPA
Kevin Dreher, NHEERL, ORD, U.S. EPA
Emily Monosson, Private Consultant to The Cadmus Group
Jeff Morris, IOAA, ORD, U.S. EPA
Stephanie Rosch, The Cadmus Group
Chon Shoaf, NCEA, ORD, U.S. EPA
John Vandenberg, NCEA, ORD, U.S. EPA
Debra Walsh, NCEA, ORD, U.S. EPA
Sally White, Student Service to NCEA, ORD, U.S. EPA
William Wilson, NCEA, ORD, U.S. EPA

External Reviewers
Pratim Biswas, Washington University
Bernard Goldstein, University of Pittsburgh
Judith A. Graham, Private Consultant
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Fred Klaessig, Degussa
Rebecca Klaper, University of Wisconsin
Terry Medley, DuPont (with David Warheit, Gary Whiting, Scott Frerichs, and Brian Coleman)
Gunter Oberdorster, University of Rochester
John A. Small, National Institute of Standards and Technology (with Richard Holbrook)
Jeffrey Steevens, U.S. Army Corps of Engineers
Mark Wiesner, Duke University (with Christine Robichaud)
Srikanth Nadadur, National Institute of Environmental Health Sciences
EPA Workgroup
Christian Andersen
Rochelle Araujo
Fred Arnold
Ayaad Assaad
Norman Birchfield
Deborah Burgin
Jim Caldwell
David Cleverly
Michele Conlon
Mary Ann Curran
Walter Cybulski
J. Michael Davis (Co-chair)
Jane Denne
Steve Diamond
Jaimee Dong
Kevin Dreher
Jeremiah Duncan
Brian Englert
Patricia Erickson
Cathy Fehrenbacher
Gina Ferreira
Kathryn Gallagher
Michael Gill
Michael Gonzalez
Maureen Gwinn
Kathy Hart
Tala Henry
Ross Highsmith
Lee Hofmann
Marion Hoyer
Joe Jarvis
Bernine Khan
David Lai
Wen-Hsiung Lee
Laurence Libelo
Diana Locke
Jacqueline McQueen (Co-chair)
David Meyer
Gregory Miller
J. Vincent Nabholz
Nhan Nguyen
Carlos Nunez
David Olszyk
Martha Otto
Scott Prothero
Kim Rogers
Nora Savage
Phil Sayre
Rita Schoeny
Walter Schoepf
Najm Shamim
Deborah Smegal
Jose Solar
Neil Stiber
Timothy Taylor
Susan Thorneloe
Dennis Utterback
Amy Wang
Eric Weber
Randy Wentsel
Doug Wolf
Contractor Editorial Support
ICF International
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                                           Preamble
 1          Engineered nanoscale materials (nanomaterials) have been described in part as having at least one
 2    dimension on the order of approximately 1 to 100 nanometers (nm) and unique or novel properties that
 3    arise from their small size.  This document is a starting point to determine what is known and what needs
 4    to be known about selected nanomaterials as part of a process to  identify and prioritize research to inform
 5    future assessments of the potential ecological and health implications of these materials. Two specific
 6    applications of nanoscale titanium dioxide (nano-TiO2) are considered: as an agent for removing arsenic
 7    from drinking water and as an active ingredient in topical sunscreen.  These "case studies" do not
 8    represent completed or even preliminary assessments, nor are they intended to serve as a basis for risk
 9    management decisions in the near term on these specific uses of nano-TiO2. Rather, the intent is to use
10    this document in developing the scientific and technical information needed for future assessment efforts.
11          The case studies are organized around the comprehensive environmental assessment (CEA)
12    approach, which combines a product life-cycle framework with the risk assessment paradigm. Risk
13    assessment relates exposure and effects information for a substance or stressor; CEA expands on this
14    paradigm by including life-cycle stages and considering both indirect and direct ramifications  of the
15    substance or stressor.  The organization of the document reflects the CEA approach:  after Chapter 1
16    (Introduction), Chapter 2 highlights stages of the product life cycle (feedstocks, manufacturing,
17    distribution, storage, use, disposal), followed by Chapter 3  on fate and transport processes, Chapter 4 on
18    exposure-dose characterization, and Chapter 5 on ecological and health effects.
19          Each chapter and some sections of chapters have lists of questions that reflect information gaps in
20    that portion of the document. For the most part, these information gaps can be thought of as research
21    needs.  Note that some of these needs are specific to the respective uses of nano-TiO2 either as a water
22    treatment agent or as an ingredient in topical sunscreen. Other research needs may apply more broadly to
23    nano-TiO2 irrespective of its application, and still other needs may apply even more widely to
24    nanomaterials in general.
25          Readers are encouraged to consider the questions listed throughout the document and offer specific
26    comments on how individual questions, or research needs, might be more precisely or accurately
27    articulated. If additional questions should be included or if information is already available  to address
28    some of the questions posed here, readers are encouraged to provide such comments as  well. These or
29    other comments on any aspect of the document should be submitted in writing in accordance with
30    instructions, including the specified time period, stated in a Federal Register notice appearing on or about
31    July 31, 2009 referring to Docket ID No. EPA-HQ-ORD 2009-0495.
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                           Chapter  1.  Introduction
      1.1. Background
 1         Engineered nanoscale materials (nanomaterials) have been described in part as having at least one
 2    dimension on the order of approximately 1 to 100 nanometers (nm) and unique or novel properties that
 3    arise from their small size (National Nanotechnology Initiative, 2006). Like all technological
 4    developments, nanomaterials offer the potential for both benefits and risks. The assessment of such risks
 5    and benefits requires information, and given the nascent state of nanotechnology, much remains to be
 6    learned about the characteristics and impacts of nanomaterials before such assessments can be completed.
 7    This document is a starting point to identify what is known and, more importantly, what needs to be
 8    known about selected nanomaterial applications - in this case, for nanoscale titanium dioxide (nano-TiO2)
 9    - to assess their potential ecological and health implications.
10         The complex properties of various nanomaterials make evaluating them in the abstract or with
11    generalizations difficult if not impossible. Thus, this document focuses on two specific uses of nano-
12    TiO2, as a drinking water treatment and as topical sunscreen. These "case studies" do not represent
13    completed or even preliminary assessments; rather, they present the structure for identifying  and
14    prioritizing research needed to support future assessments of nano-TiO2 and an approach to study other
15    nanomaterials.
16         The case studies follow the comprehensive environmental assessment (CEA) approach, which
17    combines a product life-cycle framework with the risk assessment paradigm (Davis and Thomas, 2006;
18    Davis, 2007).  In essence, risk assessment relates exposure and  effects information for a given substance
19    or stressor, and CEA expands on this paradigm by including life-cycle stages and considering both
20    indirect and direct ramifications of the substance or stressor.  Figure 1-1 illustrates the principal elements
21    in the CEA approach.  The first column of Figure 1-1 lists typical stages of a product life cycle:
22    feedstocks, manufacturing, distribution, storage, use, and disposal (including reuse or recycling, if
23    applicable).  The second column lists environmental pathways or media (air, water, soil) to which
24    nanomaterials  or associated materials (e.g., manufacturing by-products) might be released at various
25    stages of the life cycle. Within these media, nanomaterials or associated materials can be transported and
26    transformed, as well as interact with other substances in the environment, both natural and anthropogenic.
27    Thus, a combination of primary and secondary contaminants can be spatially distributed in the
28    environment (column 3, Figure 1-1).

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/"*!-!

mprehensive Environmental Assessment


Life Cycle Environmental Fate & Exposure-
Stages Pathways Transport Dose
Feedstocks ^
Manufacture
Distribution
Storage
Use
Disposal
I
Air "1 Primary 1 1
contaminants 1 | Eco,
I loafer K
_ , Human ..
So,, J SSSSn J "~"s J ^
1 I I
Analytical methods development and application

systems
ran Health
I


                                                                   Source: Adapted from Davis and Thomas (2006) and Davis (2007).
      Figure 1-1.
Basic structure of comprehensive environmental assessment (CEA) as a framework
for identifying and prioritizing research efforts.
 2          The fourth column of Figure 1-1, exposure-dose, goes beyond characterizing the occurrence of
 3    contaminants in the environment, as exposure refers to actual contact between a contaminant and
 4    organisms (i.e., biota1 as well as human populations).  Under the CEA approach, exposure
 5    characterization can involve aggregate exposure across routes (e.g., inhalation, ingestion, dermal);
 6    cumulative exposure to multiple contaminants (both primary and secondary); and various spatiotemporal
 7    dimensions (e.g., activity patterns, diurnal and seasonal changes). Dose is the amount of a substance that
 8    actually enters an organism by crossing a biological barrier.  Conceptually, dose links exposure with the
 9    last column of Figure 1-1, which refers to ecological and human health effects that can result when an
10    effective dose reaches a target cell or organ in a receptor organism or, in an ecological context, when a
11    stressor is at a sufficient level to cause an adverse response in a receptor. "Effects" encompass both
12    qualitative hazards and quantitative exposure-response relationships.
      1 The term biota is used here to refer to all organisms other than humans.
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 1          CEA involves the elaboration and synthesis of information from the elements in all five columns
 2    depicted in Figure 1-1 to systematically evaluate the direct and indirect ramifications of a nanomaterial
 3    and its by-products. Underlying the CEA elements are analytical methods that make detection,
 4    measurement, and characterization of nanomaterials in the environment and in organisms possible. Not
 5    reflected in Figure 1-1 is an essential ingredient in making CEA effective - the inclusion of diverse
 6    technical and stakeholder perspectives to ensure that a holistic view is maintained.  As an assessment or as
 7    a framework for developing a research strategy, CEA is a collective process that requires numerous
 8    participants and contributors.
 9          Other efforts have been made to assess the potential risks of nanomaterials by incorporating a life-
10    cycle perspective (e.g., Environmental Defense - DuPontNano Partnership, 2007; Shatkin, 2008; Thomas
11    and Sayre, 2005) or by using collective expert judgment methods (e.g., Kandlikar et al., 2007; Morgan,
12    2005), primarily in a risk management context.  Although the present document differs somewhat from
13    these other efforts in its purpose, namely to aid in developing a research strategy for the comprehensive
14    environmental assessment of nanomaterial risks, all of these endeavors complement and reinforce one
15    another.
      1.2.  How to  Read this Document
16          The intent of this document is to identify systematically what is known and what needs to be
17    known about nano-TiO2 to conduct an adequate assessment of such nanomaterials in the future. The goal
18    is not to provide an actual comprehensive environmental assessment or to state conclusions regarding
19    possible ecological or health risks related to nano-TiO2.
20          This document is organized around two case studies of nano-TiO2 using the CEA approach as a
21    basic framework. Although the differences between the applications of nano-TiO2 as a water treatment
22    agent versus a topical sunscreen are important, the information currently available does not allow
23    complete differentiation between the two. For example, the ecological and health effects of nano-TiO2 are
24    described in a single chapter without regard to  whether the source of nano-TiO2 is water treatment or
25    sunscreen. However, where  distinctions are possible or seem likely (e.g., in life-cycle stages such as
26    manufacturing and use), the discussion of water treatment is presented first, followed by discussion of
27    sunscreen. In some sections, the discussions are not strictly parallel, reflecting the availability of data.
28          Also important to note is that these case studies have been developed without a specific regulatory
29    objective in mind. Although the topics selected for consideration, water treatment and sunscreen, might
30    be of interest in various policy and regulatory contexts, this document is not intended to serve as a basis
31    for risk management decisions in the near term on these specific uses of nano-TiO2.  Rather, the intent is

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 1    to use this document in developing the scientific and technical information needed for future assessment
 2    efforts as input to policy and regulatory decision-making.
 3          Focusing on only two examples of nano-TiO2 applications obviously does not represent all the
 4    possible ways in which this nanomaterial could be used or all the issues that different applications could
 5    raise. Rather, by considering the commonalities and differences between two applications of nano-TiO2,
 6    research needs can be identified that apply not only to these specific applications but generally to nano-
 7    TiO2 and perhaps even more broadly to other nanomaterials. Also, additional case studies will be
 8    developed for other applications and nanomaterials so that this process can continue and research
 9    strategies to support assessment efforts can be further refined.
10          When implemented, a CEA is intended to be comparative, examining the relative risks and benefits
11    of different technological options, for example. Ultimately, a CEA of nano-TiO2 for water treatment or
12    for topical sunscreen would seek to compare these options against current water treatment practices or
13    sunscreen ingredients. However, it is beyond the scope of this document to describe the various
14    alternatives to nano-TiO2 for these  applications, given that the immediate objective is to identify and
15    prioritize research needs related to  nano-TiO2 as illustrated by the two cases under consideration. Readers
16    seeking comparative assessments of topical  sunscreen products, with or without nano-TiO2, may wish to
17    consult evaluations by the Scientific Committee on Consumer Products (SCCP) (2007) and the
18    Environmental Working Group (EWG) (2009). The EWG analysis  in particular takes a broad  view that is
19    consistent with the CEA approach in referring to the product life cycle  and noting potential ecological as
20    well as human health considerations.
21          That this draft document is a work in progress also should be noted. New, pertinent information
22    seems to appear daily, and readers are encouraged to provide information bearing on the case studies and,
23    in particular, to identify additional  research needs and refine the questions listed throughout this
24    document.  The document, however, is not intended to provide an exhaustive review of the literature, and
25    focuses instead on findings most clearly relevant to assessment objectives.
26          Finally, the information presented in this document was obtained from a variety of published and
27    unpublished sources, including corporate Web sites and personal communications, as well as inferences
28    based on information about other materials or applications.
      1.3.  Terminology
29          This document focuses on nano-TiO2 particles primarily in the size range of 1 to 100 nm. Where
30    information is not specific to nanoscale particles, TiO2 may be referred to without the "nano" prefix.  To
31    make an explicit distinction between the nanoscale material and other forms of TiO2 not having the

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 1    special characteristics of nano-TiO2, the term "conventional" is used.2  Even so, conventional materials
 2    will often contain a range of particle sizes, including a fraction with nanoscale dimensions. Conversely,
 3    as discussed in more detail below, in many circumstances primary nanoscale particles can aggregate or
 4    agglomerate into secondary particles with dimensions greater than 100 nm. However, it is not clear that
 5    once a cluster of primary nano-TiO2 particles exceeds 100 nm their properties become like those of
 6    conventional TiO2.  For example, inhalation of nano-TiO2 (20 nm diameter) induced more pulmonary
 7    inflammation in the rat than inhalation of fine TiO2 (about 250 nm diameter) at a similar mass
 8    concentration, even though particles in both groups had similarly sized agglomerates (0.71 micrometer
 9    [um] mass median aerodynamic diameter [MMAD] nano; 0.78 urn MMAD fine) (Oberdorster et al.,
10    1994; Oberdorster, 2000). Additional analysis revealed that effects were similar when expressed on the
11    basis of surface area.  Whether the constituent primary particles necessarily remain agglomerated or
12    aggregated if conditions change also is not clear. As will be discussed under Fate and Transport (Chapter
13    3), disaggregation can occur under some conditions.  Given these considerations, this document does not
14    use 100 nm as a definitional hard line in considering what might be relevant to an evaluation of nano-
15    TiO2. This view is consistent with a statement by the European Commission (2008) that extends the term
16    nanomaterial to encompass "nanostructured materials," defined by the International Organization for
17    Standardization (ISO) (Technical Specification [TS]  27687) as "[aggregates and agglomerates, often
18    existing at a micro size, [that] may have some of the behaviour and effects of their smaller sub units,  e.g.,
19    due to an increased surface area."
20          Degussa AEROXIDE® P25 (hereafter referred to as P25) is a commercial-grade, uncoated nano-
21    TiO2 product that has been studied extensively and referenced in the literature and is therefore often
22    mentioned in later sections of this document.  As discussed below, however, P25  does not represent all
23    nano-TiO2 preparations and should not be equated with the generic term nano-TiO2.
      1.4.  Conventional Ti
24          Although this document focuses on nano-TiO2, highlighting some facts about conventional
25    titanium dioxide (TiO2) first is instructional. Also known as titania, TiO2 has been used commercially
26    since the early 1900s in numerous consumer and industrial applications, particularly coatings and
27    pigments.  TiO2 is a naturally occurring mineral that can exist in three crystalline forms, known as rutile,
28    anatase, and brookite, and in amorphous form.  Rutile is the most common form of TiO2 found in nature.
      2 The terms "bulk" and "pigmentary" are also often used to distinguish conventional from nanoscale TiO2.
       Additionally, terms such as ultrafine, PM-0.1, micronized, and attenuation-grade have been used to denote
       nanoscale particles, but usually in a particular context or field of specialization such as aerosols and air pollution.
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 1    Elemental titanium is also found in ilmenite (FeTiO3) and other minerals and ores, and TiO2 can be
 2    produced by processing of these minerals and ores. TiO2 is insoluble in water, hydrochloric acid, nitric
 3    acid, and ethanol, but soluble in hot concentrated sulfuric acid, hydrogen fluoride, and alkali (NRC,
 4    1999). TiO2 is used to increase the whiteness or opacity of many consumer products, such as paints,
 5    coatings, plastics, paper, printing inks, roofing granules, food, medicine, toothpaste, cosmetics, and skin
 6    care products, including topical sunscreens. In the United States, surf ace-mining operations in Virginia
 7    and Florida produce concentrated titanium-containing minerals  (ilmenite and rutile) suitable as feedstock
 8    for TiO2 production (U.S. Geological Survey, 2009).  Other countries that produce significant amounts of
 9    titanium ores include Australia, Canada, China, India, Norway, and South Africa (U.S. Geological Survey,
10    2009).
11          With exposure to ultraviolet (UV) radiation (wavelengths less than -400 nm), pure TiO2 is
12    photocatalytic.  Studies suggest anatase and rutile have different photocatalytic properties, with anatase
13    being the more reactive (Sayes et al., 2006; Uchino et al., 2002). In applications such as paints, coatings,
14    and cosmetics, where chemical stability is required, the photocatalytic properties of TiO2 are often
15    suppressed by coating the particles with silica and alumina layers. On the other hand, the photocatalytic
16    properties of TiO2 are increasingly exploited in a number of other experimental and commercial
17    applications, including degradation of organic compounds, microbiological organism destruction, and
18    conversion of metals to less soluble forms in waste water, drinking water, and indoor air.  For more
19    information on conventional TiO2, please see the article by Diebold (2003) and the bulletin published by
20    the National Institute for Occupational Safety (NIOSH) (2005).


      1.5. Nano-TiOz
21          One of the main differences between nano-TiO2 and conventional TiO2 is the much greater surface
22    area of a given mass or volume of nanoparticles compared to an equivalent mass or volume of
23    conventional TiO2 particles. To illustrate, a 5-nm particle would have a volume of 65 cubic nm (4/3 n r3)
24    whereas a 500-nm particle would have a volume  of 65,000,000  cubic nm.  Therefore, one million 5-nm
25    particles would be required to equal the volume of a 500-nm particle. The surface area of a 5-nm particle
26    equals approximately 80 square nm (4 n r2), whereas the surface area of a 500-nm particle equals
27    approximately 800,000 square nm.  Multiplying the surface area of the 5-nm particle by one million (the
28    number of 5-nm particles needed to equal the volume of a 500-nm particle) yields a total surface area of
29    approximately 80,000,000 square nm, which is 100-fold greater than the surface area of the 500-nm
30    particle. This greater relative surface area of the nano-TiO2 particles affords a greater potential for
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  1     properties such as catalytic activity and UV absorption at certain wavelengths (Shao and Schlossman,
  2     1999).
  3           Such properties have led to the development or use of nano-TiO2 for a wide variety of applications,
  4     including self-cleaning surface coatings,  light-emitting diodes, solar cells, disinfectant sprays, sporting
  5     goods, and the subjects of this document, water treatment agents and topical sunscreens.  Before
  6     considering specific applications of nano-TiO2, some fundamental issues related to characterization of this
  7     material should be noted.
  8           Not all nano-TiO2 is the same.  Commercially available brands of nano-TiO2 can vary in particle
  9     size, surface area, purity (e.g., due to doping, coating, or quality control), surface characteristics,
10     crystalline form, chemical reactivity, and other properties (see Table 1-1). Nano-TiO2 is available in pure
11     anatase, pure rutile, and mixtures of anatase and rutile.  In general, anatase nano-TiO2 is more
12     photocatalytic than the rutile form, and nanoscale rutile is less photoreactive than either anatase and rutile
13     mixtures or anatase alone (Sayes et al., 2006).  However, a mixture  of 79% anatase and 21% rutile nano-
14     TiO2 (P25) was found to be more photocatalytic than 100% anatase nano-TiO2 in some instances
15     (Coleman et al., 2005; Uchino et al., 2002), but less effective in others (Nagaveni et al., 2004). Such
16     contrasts point to the role of other factors in accounting for the behavior and effects of nano-TiO2.  For
17     example, surface treatment of nano-TiO2 can change nano-TiO2 activity, including photoreactivity.
18     Aeroxide T805, which is nano-TiO2 that  has been treated with trialkoxygoctyl silane on the surface, has
19     very low surface reactivity (Degussa,  2003). Similarly, surface coatings of silicone and other compounds
20     are used to decrease nano-TiO2 photoreactivity so that nano-TiO2 can be used to protect human skin,
21     plastic, and other objects from UV radiation.


            Table 1 -1.  Examples of nano-TiCh physicochemical properties.

            Agglomeration / aggregation status in     Particle size and size distribution         Shape / aspect ratio (e.g., width and
            the relevant media                   „,  .   ....   .. ..                  length)
                                            Photocatalytic activity
            Bulk density / particle density                                           Surface area / specific surface area
            Composition / surface coatings                                          Surface charge / zeta potential
            Crystal structure / crystallinity                                           Surface chemistry
            (crystalline phase, crystallite size)        Purity of sample
                                            _,.,......,             Surface contamination
            Dustiness                         Radical formation potential
                                            _  ,    ....                     Surface reactivity
            Octanol-water partition coefficient        Redox potential
                                                                            Water solubility

            Source: Data from Department for Environment, Food, and Rural Affairs (2007); Powers etal. (2006); Powers etal. (2007); Warheitetal.
                (2007c); and Organisation for Economic Co-operation and Development (OECD) (2008).
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 1          External factors can also influence photoreactivity. Krishna and coauthors (2006), for example,
 2    found that the presence of fullerenes, which scavenge photogenerated electrons, enhances the
 3    photocatalytic efficacy of nano-TiO2.  Likewise, Komaguchi and colleagues (2006) saw significant
 4    increases in photocatalytic efficiency of P25 after exposure to an oxidizing environment.
 5          Photocatalytic nano-TiO2 is preferred for water treatment, and photostable nano-TiO2 is preferred
 6    for sunscreen use.  Some sunscreens, however, contain photoreactive nano-TiO2. Although pure uncoated
 7    and undoped anatase TiO2 is photocatalytic, and uncoated and undoped rutile TiO2 is generally
 8    photostable, there is no quick way to identify the photoreactivity of other nano-TiO2.  For example,
 9    although doped rutile nano-TiO2 can be extremely photostable (Reisch, 2005), rutile nano-TiO2 produced
10    by a certain specific powder-preparation method can be highly photocatalytic (Kim et al., 2003b).
11    Similarly, not all coatings decrease nano-TiO2 photoreactivity.
12          Due to various degrees of porosity, nano-TiO2 particles with the same diameter can differ in surface
13    area.  Because nano-TiO2 reactivity and consequently behavior and effects are influenced by many nano-
14    TiO2 physicochemical properties, two nano-TiO2 products with the same reported (but limited) parameters
15    should not be assumed in fact to be  equivalent. For instance, a manufacturer might use the same core
16    nano-TiO2 for surface-treated and untreated nano-TiO2, and both might have the same particle size and
17    surface area, but differ in reactivity, as in the case of P25 and Aeroxide T805.
18          Another characteristic of significance is the aggregation or agglomeration of nano-TiO2 particles.3
19    According to one industrial manufacturer of nanoscale titania produced through flame hydrolysis (see
20    Section 2.2 for a description of this  manufacturing technique and others), "tests and calculations have
21    shown that free primary particles  with dimensions of less than 100 nm only exist in [flame] reactors for a
22    few milliseconds" (Degussa, 2009). Aggregates of nano-TiO2, sometimes referred to  as "colloidal," are
23    often roughly an order of magnitude greater in size than primary particles (Dunphy Guzman et al., 2006;
24    Kormann et al., 1988; Lecoanet et al., 2004).  The mean aggregated particle diameter  of P25 is about
25    3.6 (im, with the smallest 4% of particles having an average diameter of 160 nm (Klaessig, 2006). After
26    being subjected to sonication for  10 minutes, the smallest 15% of P25 particles averaged an agglomerate
27    diameter of 160 nm, while the 50th  percentile diameter was 1.6 (im, roughly two orders of magnitude
28    larger than the reported primary particle size of P25, which is 21 nm  (Degussa, 2007;  Wahi et al., 2006).
29    Ridley et al. (2006) observed that a  suspension of uncoated nano-TiO2 anatase from Ishihara Techno
30    Corporation (Osaka, Japan) with primary particles of 4-nm diameter consisted mainly of aggregates in the
      3 Aggregation generally involves fusion or sintering of particles, while agglomeration involves a weaker bond.  Use
       of these terms, however, has not been standardized, and in many cases the degree of bonding is unknown.
       Consequently, the terms are often used together in this document where it is not clear which would be more
       appropriate.

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 1    1- to 30-(im diameter range, and that these size ranges persisted even under sonication and other
 2    conditions that would favor disaggregation.
 3          Despite the presence, and sometimes the predominance, of such large particles, several researchers
 4    investigating laboratory-synthesized anatase and commercial nano-TiO2 products such as P25 have also
 5    found free particles or aggregates with diameters less than 100 nm in varying amounts, depending on
 6    synthesis method, temperature, solution pH, and the presence of buffers (Jiang et al., 2009).  Moreover,
 7    some preparations are  specifically designed to generate dispersed particles (e.g., Seok et al., 2006), which
 8    would be important in using nano-TiO2 as a catalyst.
 9          The pHpzc of a nanoparticle (the pH at the "point of zero charge," where the net electric charge at
10    the particle surface is zero) has important ramifications for aggregation, because at that pH particles will
11    fail to electrostatically repel each other.  In laboratory studies, the size range of aggregates and the
12    presence of free nano-TiO2 particles (synthesized on-site,  ranging from 5 to 50 nm) were found to be pH-
13    dependent: when the solution pH differed from the pHpzc of the particles, the aggregates tended to be
14    smaller (Dunphy Guzman, pers. comm., 2007; Dunphy Guzman et al., 2006). Sampled aggregates ranged
15    up tolSO nm in size, and contained an estimated 8 to 4,000 nanoparticles (Dunphy Guzman et al., 2006).
16    The pHpzc also depends at least in part on the crystallinity of the nano-TiO2 particles:  Finnegan et al.
17    (2007) reported pHpzc values of ~5.9 for rutile and ~6.3 for anatase.
18          Coatings and surface treatments also affect particle aggregation/agglomeration behavior. A
19    preliminary report by Wiench and colleagues indicated that coated nano-TiO2 particles (rutile, size 50 x
20    10 nm, surface area of 100 square meters per gram [m2/g]; coatings included combinations of aluminum
21    hydroxide, hydrated silica, and various polymers) had slower agglomeration and sedimentation rates and
22    a larger fraction of primary nanoparticles remaining in the sample compared with uncoated particles (20
23    to 30 nm, anatase/rutile 80/20, surface area 48.6 m2/g) (Wiench et al.,  2007).
24          The complexity of nano-TiO2 characterization is illustrated in Table 1-2, from Warheit et al.
25    (2007a).  The chemical composition of three different types of ultrafine TiO2 manufactured by DuPont
26    was determined by X-ray fluorescence.  The cores of all three types of nano-TiO2 were TiO2, but the
27    crystalline form and the surface coating  of alumina or silica differed. Each type of particle was said to
28    exhibit a mean diameter of approximately 140 nm but with (unspecified) fractions of the size distributions
29    below 100 nm. The chloride ions on the surface of the particles were neutralized during production.
30    (Other effects these materials cause are described in Chapter 5.) As shown in Table 1-2, the surface area,
31    crystallinity, chemical reactivity, surface coating, particle  size distribution, and pH varied for the
32    materials, all three of which were nominally nano-TiO2.
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      Table 1-2. Characterization of three nano-TiOz particle types.3
Particle
Type
Uf-A
Uf-B
Uf-C
BET Surface
Area (m2/g)
18.2
35.7
38.5
Chemical Composition
98%Ti02(100%rutile),2%
alumina
88%Ti02(100%rutile),5%
alumina, 7% silica
92%Ti02(79%rutile;21%
anatase), 7%alumina, 1%silica
Chemical Median Particle Size and Size Range c
Reactivity > jnWater jnpBS
10.1 136nm±35% 1990nm±25%
1-2 UQ50%]± 2669nm±25%
0.9 140nm±44% -
pH in
Deionized
Water
5.64
7.14
4.80
      a BET - Brunauer, Emmett, Teller method of calculating surface area
      PBS - Phosphate buffered saline
      b Chemical reactivity was tested using a Vitamin C (antioxidant) yellowing assay.
      c After sonication for 15 min at 60 Hertz (Hz).
      Source: Modified with permission from Warheit et al. (2007a).
  1          The characteristics of a nano-TiO2 product might change over time.  Using a custom-made anatase
 2    nano-TiO2 formulation (uncoated) with a range of particle sizes, Kolaf et al. (2006) found that average
 3    particle sizes increased over time, due to both agglomeration and re-crystallization (smaller particles
 4    dissolving in the aqueous medium and their constituent molecules then adding to the mass of the larger
 5    particles).  Over the course of 8 years, average (mode) particle size increased from about 10 nm to about
 6    14 nm.  The investigators also observed that overtime relative surface area decreased, light energy
 7    absorbance characteristics changed, and perhaps most surprisingly, photocatalytic performance improved,
 8    even as relative surface area decreased.
 9          As discussed in greater detail in Chapter 5 (Section 5.1.1), these and other issues have been  noted
10    in various recommendations for improving the characterization of nanomaterials in exposure and
11    ecological as well as health effects studies.  In general, however, reports of toxicity and exposure studies
12    of nano-TiO2, especially those conducted prior to the year 2000, have not been sufficiently  attentive to the
13    issues described above.  Manufacturers' literature often has been accepted as having described their
14    products under all conditions - an oversimplification at best. Additionally, attempts to characterize
15    nanoscale particle sizes and size distributions in relation to toxicity and exposure evaluations have been
16    prone to errors involving non-representative sampling, agglomeration during sample preparation,
17    contamination and degradation during product storage, measurement methods, and conditions under
18    which the study was conducted (Powers et al., 2007). Further, some particle characterization techniques
19    can affect measurement accuracy, suggesting that more than one technique might be necessary to describe
20    particle sizes accurately. Accurate characterization is clearly important if the behavior and  effects  of
21    nano-TiO2 are to be understood, predicted, and related to other materials (both nanoscale and
22    conventional).
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      1.5.1. Water Treatment
 1          This document assumes that nano-TiO2 would be used specifically for arsenic removal in a
 2    drinking water treatment facility. In addition to arsenic removal (Li et al., 2009), however, nano-TiO2
 3    could be used for disinfection of pathogens (Alrousan et al., 2009; Coleman et al., 2005; Li et al., 2008a;
 4    Rincon and Pulgarin, 2003) or for remediation of ground water or waste water contaminated with various
 5    organic and inorganic pollutants (Adams et al., 2004; Chen and Ray, 2001; Han et al., 2009; Kim et al.,
 6    2003a; Lee et al., 2008; Lin and Valsaraj, 2003; Ryu and Choi, 2008; Xu et al., 2009b). The latter use
 7    would pose rather different scenarios of environmental releases and fate and transport, and would add
 8    considerably to the complexity of this document.  Therefore, the case  study of nano-TiO2 for water
 9    treatment has been limited to the consideration to arsenic removal in water treatment facilities.
10          Most of the relevant literature to date has reported laboratory tests of nano-TiO2 as a photocatalytic
11    treatment for conversion of arsenite [As(III)] to arsenate [As(V)], a species that is more easily removed in
12    water treatment because of its lower solubility in typical drinking water treatment conditions (e .g., Dutta
13    et al., 2004; Ferguson et al., 2005; Pena et al., 2006). Although neither conventional TiO2 nor nano-TiO2
14    is known to have been used in a full-scale drinking water treatment plant, both conventional TiO2 and
15    nano-TiO2 as photocatalytic agents have been pilot-tested in dinking water treatment plants (Dionysiou,
16    pers. comm., 2009; Pichat, 2003; Purifies Solutions, 2008; Richardson et al., 1996).
17          For arsenic removal from water, both conventional and nanoscale TiO2 have been developed to
18    photocatalytically oxidize arsenic and absorb arsenic.  Studies have shown that TiO2 can oxidize As(III) to
19    As(V) and adsorb inorganic arsenic (Dutta et al., 2004; Fostier et al., 2008; Hristovski et al., 2007). The
20    mechanism for TiO2 photocatalytic oxidation of As(III) has been suggested to be through the generation
21    of superoxide ions, and the major oxidant species might be hydroxyl radicals ('OH) (Sharma and Sohn,
22    2009). Recently, nano-TiO2 was shown to mineralize methylated arsenic and to adsorb methylated
23    arsenic (Xu et al., 2007; Xu et al., 2008). Both dimethylarsinic acid [DMA(V)] and monomethylarsonic
24    acid [MMA(V)] were readily mineralized to As(V) by transforming the methyl group into organic
25    compounds such as methanol, formaldehyde, and formic acid. Dimethylarsinic acid was
26    photocatalytically oxidized into MMA(V), which was subsequently oxidized into As(V).  Hydroxyl
27    radicals could be the primary oxidant (Xu et al., 2007; Xu et al., 2008).
28          The mechanism of arsenic adsorption onto TiO2 surfaces was through the formation of bidentate
29    inner sphere complexes for As(V), As(III), and MMA(V), and forming monodentate inner sphere
30    complexes for DMA(V) (Jing et al., 2004; Jing et al., 2005a; Jing et al., 2005b; Pena et al., 2006). In
31    ground water containing As(III), As(V), MMA(V), and DMA(V), nano-TiO2 adsorbs As(III) and As(V)
32    most, followed by MMA(V), but almost no DMA(V) (Jing et al., 2009). The difference in competitive
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 1    adsorption could be due to lower stability of the monodentate surface structure formed between TiO2 and
 2    DMA(V) than that of the bidentate structure formed between TiO2 and other arsenicals.
 3          Photocatalytic oxidation is also the mechanism for TiO2 degradation of organic pollutants in waste
 4    water.  Photocatalytic degradation is based on the formation of radicals (hydroxyl radicals, superoxide
 5    radical anions [O2 ], and hydroperoxyl radicals [-OOH]), which serve as oxidizing species in the
 6    photocatalytic oxidation process (Lu et al., 2009).  Hydroxyl radicals, the most powerful oxidants TiO2
 7    produces in the photocatalysis, can act on organic contaminants present at or near the surface of TiO2
 8    (Bianco Prevot et al.,  1999).
 9          One generally accepted mechanism of nano-TiO2 antimicrobial property is the generation of
10    reactive oxygen species (ROS), which can cause cell wall or cell membrane damage (Kuhn et al., 2003;
11    Neal, 2008), such as lipid peroxidation (Maness et al., 1999).  Although UV illumination increases
12    photocatalytic nano-TiO2 toxicity to bacteria and fungi, photocatalytic nano-TiO2 is also toxic in the dark
13    (Adams et al., 2006; Coleman et al., 2005). Because TiO2 generates ROS  (mainly highly reactive
14    hydroxyl radicals) in the presence of UV and oxygen (Reeves et al., 2008), mechanisms other than
15    oxidative stress might also contribute to nano-TiO2 toxicity in the dark (and possibly also under UV), as
16    suggested by a recent study indicating that anatase nano-TiO2 can generate carbon-centered free radicals
17    in the dark in the presence of dissolved oxygen (Fenoglio et al., 2009).


      1.5.2. Sunscreen
18          Nano-TiO2 formulations of sunscreen have proven popular because they appear transparent on the
19    skin; formulations using conventional TiO2 or other inorganics such as zinc oxide (ZnO) (Schlossman et
20    al., 2006) create a milky white appearance. Nano-TiO2 serves as a sunscreen in two ways, by absorption
21    and scattering, depending on the wavelength of UV light. UV-B wavelengths are in the range of 290-320
22    nm, and are primarily absorbed by nano-TiO2; UV-A wavelengths are in the range of 320-400 nm, and are
23    primarily scattered by nano-TiO2 (Shao and Schlossman, 1999). Optimal scattering is thought to occur
24    when the diameter of the particles is approximately half the wavelength of the light to be scattered
25    (Fairhurst and Mitchnick, 1997; Klaessig, 2009); also see Appendix A for more  information on how nano-
26    TiO2 particle size relates to UV-A and UV-B protection). Information on chemical and other properties of
27    topical sunscreens containing nano-TiO2 can be found in Appendix A.
28          Conventional TiO2  absorbs and scatters UV radiation, making it an effective active ingredient in
29    sunscreens.  Like ZnO, TiO2 is a "physical blocker" of UV radiation, as opposed to many chemically
30    active ingredients that serve as "chemical filters," such as avobenzone and benzophenone, which in some
31    individuals can cause adverse skin reactions, including blisters, itching, and rash (U.S. EPA, 2006d).

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 1    Thus, sunscreens containing physical blockers have long been an attractive option to those with sensitive
 2    skin. Apart from this niche market, the use of TiO2 in sunscreen was historically limited because of
 3    aesthetic considerations. Because conventional TiO2 scatters visible light, it remains visible as a white
 4    film when applied on skin.  With the advance of technology to produce transparent nanoscale TiO2
 5    particles, which scatter very little visible light and therefore appear transparent when applied on skin,
 6    nano-TiO2 has entered the mainstream as an active ingredient in sunscreens and has also been added to
 7    numerous other cosmetic products to provide UV protection. With exposure to UV radiation
 8    (wavelengths less than ~ 400 nm), pure anatase  nano-TiO2 is photocatalytic.  In sunscreen, however,
 9    photocatalysis is an undesirable property that can be addressed by applying surface treatments to the
10    crystals, selecting a less photoreactive form (rutile), or adding antioxidant ingredients to the formula.
11          The maximum concentration of TiO2 in sunscreen that the U.S. Food and Drug Administration
12    (FDA) allows is 25% (FDA, 1999), but this limit does not distinguish between conventional and nano-
13    scale TiO2, between anatase and rutile, or between coated and uncoated particles.  The concentrations
14    actually used, according to product labels, typically range from 2% to 15% (see Table A-l, Appendix A).
15    Europe, Australia, Canada, and South Korea also have approved the use of TiO2 as a UV filter in
16    sunscreen with a maximum concentration of 25%.  Japan does not regulate TiO2 as a UV filter in
17    sunscreen (Oxonica, 2005; Risk & Policy Analysts Limited, 2004; Steinberg, 2007).
18          Some TiO2-bearing sunscreens are explicitly labeled as containing nanoparticles. Others are
19    labeled as containing "micronized" TiO2, a grade commonly used in cosmetics.  "Micronized" implies a
20    particle size of about 1 micron (or micrometer, which is one order of magnitude larger than 100 nm), but
21    how precisely manufacturers use the term is unclear.  Sometimes "micronized" is taken to imply a nano
22    size range (e.g., Shao and Schlossman, 1999), and sometimes it is considered distinct from nano (e.g.,
23    Environmental Working Group, 2008). In the latter case, TiO2 with a mean particle size of several
24    micrometers is still very likely to include a significant fraction of particles in the nano size range.  Even
25    sunscreens using pigment-grade TiO2 likely contain a proportion of nano-sized particles. When
26    Consumer Reports tested seven leading national sunscreens labeled as containing ZnO or TiO2 or both,
27    but with no indication on the container regarding the presence of nanoparticles, they found nanoparticles
28    in all seven products (Anonymous, 2007; La Farge, 2007). (They also confirmed the presence of
29    nanoparticles in an eighth brand labeled as  containing nanoparticles.)  No information was available,
30    however, on the quantities or sizes of the nanoparticles detected in any of these sunscreens (La Farge,
31    2007). Due to concerns over consumer acceptance of nanotechnology, some nano-TiO2 sunscreens might
32    simply be labeled as containing "titanium dioxide."
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      1.6.  Analytical Methods
 1          Sensitive and accurate analytical methods for nanomaterials are critical tools for nanomaterial risk
 2    assessment, because measurement and characterization of nanomaterials, alone and in various media, are
 3    required for properly assessing exposure, conducting toxicological studies, estimating dose-response
 4    relationships, and understanding the behavior and effects of nanomaterials.
 5          Section 1.4 addressed the aspects of characterization generally needed for nanomaterials,
 6    particularly nano-TiO2. This section provides a brief review of analytical methods that could be suitable
 7    for nano-TiO2, with a focus on currently available methods. Because nano-TiO2 is not radio-labeled and
 8    does not fluoresce, analytical methods based on these two attributes are not relevant. Additionally, the
 9    importance of chemical analysis of nanomaterials is acknowledged (such as for identifying their
10    molecular components and for characterizing certain surface properties), but these methods also are not
11    discussed in this section.  Some of the chemical analysis methods suitable for nanomaterials are discussed
12    in (Powers et al., 2006; U.S. EPA, 2008c). For detailed comparison of various methods, readers are
13    referred to review articles by Maynard and Aitken (2007), Powers et al. (2006; 2007), and Domingos et
14    al. (2009b).
      1.6.1. Methods for Laboratory Research
15          The physicochemical properties of nano-TiO2 can change over time (Kolaf et al., 2006) and in
16    various milieux; therefore, the characteristics of engineered nanomaterials at the point of production could
17    be vastly different after transport, storage, and preparation for testing. Nanomaterials used in
18    toxicological testing ideally would be characterized by analyzing the raw material (as received from the
19    manufacturer or supplier); nanomaterials in the testing media for the duration of the experiment; and
20    nanomaterials (and possibly degraded products or biotransformed products) in the biological samples
21    being tested, such as in urine, organs, and cells.
22          The equipment and methods for measuring nanomaterials in the laboratory are numerous and are
23    evolving. In addition to methods that can be used for characterizing nanomaterials in aerosols and liquids
24    (including biological fluids) (Table 1-3) (Maynard and Aitken, 2007; Nanosafe, 2008b; Powers et al.,
25    2006; Powers et al., 2007) and methods  specific for radio-labeled or fluorescent nanomaterials, the
26    following methods have been used on biological samples:  transmission electron microscopy (TEM),
27    electron-dispersive X-ray analysis (EDS), and inductively coupled plasma mass spectroscopy (ICP-MS)
28    for presence and location; dynamic light scattering (DLS) in conjunction with TEM for size (both core
29    and shell); high resolution transmission electron microscopy (HRTEM) for crystalline structure;


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1    inductively coupled plasma atomic emission spectroscopy (ICP-AES) for elemental composition and
2    quantitative nanomaterial uptake; video-enhanced differential interference contrast (VEDIC) microscopy
3    for uptake and localization (Marquis et al., 2009); and scanning probe microscopy (SPM) for size and
4    three-dimensional images (Gwinn, accepted for publication).  ICP, X-ray diffraction (XRD), and nuclear
5    magnetic resonance (NMR) can be used to determine chemical composition (Gwinn, accepted for
6    publication). The combination of flow field flow fraction (FIFFF) and ICP-AES has been used to detect
7    nano-TiO2 in the tested commercial sunscreen, with information on mass-size distribution and Ti content
8    of extracted nano-TiO2 from sunscreen.
       Table 1-3.  Analytical methods for characterizing nanomaterials in aerosol and in liquid.
Metric
Number




Surface area



Mass




Size












Method
Condensation particle counter (CPC)
Scanning mobility particle sizer (SMPS)
Electrical low pressure impactor (ELPI)
Optical particle counter (OPT)
Electron microscopy (EM)
Scanning mobility particle sizer (SMPS)
Electrical low pressure impactor (ELPI)
SMPS and ELPI used in parallel
Diffusion charger
Size selective personal sampler
Size selective static sampler
Tapered element oscillating microbalance (TEOM®)
Scanning mobility particle sizer (SMPS)
Electrical low pressure impactor (ELPI)
Dynamic light scattering (DLS)
Centrifugal sedimentation
Laser diffraction/static light scattering
Low pressure impacter and electrical low pressure impactor (ELPI)
Scanning/differential mobility analysis
Field flow fractionation (FFF)
Size exclusion chromatography (SEC)
Acoustic techniques
Electron microscopy (EM)
Scanning probe microscopy (SPM)
Time of flight mass spectroscopy
Atomic force microscopy (AFM)
Specific surface area (Brunauer, Emmett, Teller [BET], titration, diffusion
charging)
Aerosol
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Maybe
No
Yes
Yes
Yes
No
No
No
No

Yes
No
Yes
Liquid
-
-
-
-
-
-
-
-
-
-
-
-
-
-
Yes
Yes
Yes
No
No
Yes
Yes
Yes
Possible with cryo-
techniques
Yes
No
Maybe
Titration techniques only
        Source: Modified with permission from Maynard and Aitken (2007), Powers et al. (2006), Powers et al. (2007), and data from Nanosafe (2008a).
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      1.6.2. Methods and  Instrumentation to Assess Environmental
      Occurrence
 1          Detecting nanoparticles in the environment can be difficult because available analytical methods
 2    often are not sensitive enough for current environmentally relevant concentrations and cannot distinguish
 3    natural materials in the nanoscale size range from manufactured nanomaterials (Domingos et al., 2009b;
 4    Englert, 2007; Simonet and Valcarcel, 2009). Also, many analytical methods require sample treatment
 5    and extraction (Englert, 2007), which may include solvent evaporation, and consequently could cause
 6    nanoparticle aggregation and salt precipitation (Simonet and Valcarcel, 2009).  Detecting nanoparticles in
 7    water or soil is further complicated by the heterogeneous nature of the samples. Ideally such
 8    measurements would be done in situ to avoid changes in nanoparticles (such as agglomeration) due to
 9    different conditions in the immediate milieu, but portable equipment sufficiently sensitive to detect
10    nanoparticles at environmentally relevant concentrations has not yet been developed (Simonet and
11    Valcarcel, 2009).
12          Analytical methods that are currently available for nanomaterials in soil, sediment and ground
13    water were summarized in arecentU.S. Environmental Protection Agency (U.S. EPA) State of Science
14    Review (U.S. EPA, 2008c) (Table 1-4).  Methods can be coupled to enable detection of more than one
15    parameter at a time. For example, FIFFF can be coupled with ICP-MS for both size and chemical
16    analysis.
17          In a study comparing six analytical methods for determining nanomaterial sizes [TEM, atomic
18    force microscopy (AFM), DLS, fluorescence correlation spectroscopy, nanoparticle tracking analysis, and
19    flow field flow traction], Domingos et al. (2009b) concluded that the two most commonly used
20    techniques reported in the literature [electron microscopy (EM) on air-dried samples and DLS] were also
21    the two techniques that appear to be most prone to artifacts.  Using multiple analytical techniques or
22    multiple preparation techniques, or both, has been recommended (Domingos et al., 2009b; Englert, 2007).
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           Table 1-4. Analytical methods for nanomaterials in soil, sediment, and ground water for
                      size fraction and distribution, surface area, and phase and structure.
                Metric
               Analytical method
Sample type
           Size fractionation   Centrifugation
                           Ultrafiltration - direct-flow ultrafiltration or tangential-flow
                           ultrafiltration (IFF)
                           Field flow fractionation (FFF)
                           Capillary electrophoresis (CE)
                           Size exclusion chromatography (SEC)
                                              Aquatic colloids and particles extracted
                                              from soil and sediment samples.
                                              Nanoparticles must be in solution.
           Size distribution    Transmission electron microscopy (TEM)
                           Scanning electron microscopy (SEM)
                           Scanning probe microscopy (SPM)
                           Dynamic light scattering (DLS)
                           Laser-induced breakdown detection (LIBD)
                           Small- and wide-angle X-ray scattering (SAXSM/AXS)
           Surface area       Brunauer, Emmett, Teller method (BET)
                           Calculation from transmission electron microscopy (TEM)
                           (length and width) and atomic force microscopy (AFM)
                           (height) measurements, and particle nanocrystalline
                           geometries
                                              Only nanomaterials with a regular or
                                              pseudo-regular geometry and without
                                              significant porosity
           Phase and
           structure
Electron diffraction
X-ray diffraction (XRD)
X-ray absorption spectroscopy (XAS)
Raman spectroscopy
           Source: Data from U.S. EPA (2008c).


      1.6.3. Methods and  Instrumentation to Assess Workplace Exposure

 1          Workplace exposure thus far has focused on measuring nanoparticles in the air.  Instruments that
 2    can be used for aerosol sampling are available, but most instruments for aerosol sampling are designed for
 3    laboratory use (Nanosafe, 2008b) and lack one or more the following desired attributes:  portability, ease
 4    of use, capacity to distinguish nanoparticles from non-nanoparticles, different size bins in the 1- to
 5    100-nm range, or ability to sample personal breathing zones (Ostraat, in press).
 6          Several governmental and non-governmental organizations have begun addressing the need for
 7    equipment and methods for monitoring nanomaterials, particularly nanoaerosols,  in the workplace. For
 8    example, NIOSH recently published a document titled Approach to Safe Nanotechnology - Managing the
 9    Health and Safety Concerns Associated with Engineered Nanomaterials (NIOSH, 2009), in which
10    sampling and monitoring methods and equipment are discussed. Nanoparticle Occupational Safety and
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 1    Health Consortium (NOSH), an industry-led consortium of participants from academia and governmental
 2    and non-governmental organizations, is helping to define best practices for working safely with
 3    engineered nanoparticles (NOSH, 2008; Ostraat et al., 2008). The NOSH Consortium has developed
 4    portable air monitoring methods intended for daily monitoring in nanoparticle research and development
 5    or in manufacturing settings.
 6          Maynard and Aitken (2007) summarized available devices and approaches for evaluating numbers,
 7    surface areas, and mass concentrations of nanoparticles  for monitoring aerosol exposure.  In 2008, the
 8    NanoSafe2 project, a European Community-sponsored project for safe production and use of
 9    nanomaterials, released a report that highlighted findings in measurement methodologies for nanoparticle
10    detection and measurement with various types  of on-line and off-line monitoring instruments (Nanosafe,
11    2008b).  The report provided examples of new  nanoaerosol measurement equipment that is easy to
12    transport and use.  No commercially available equipment, however, is currently available  for long term
13    monitoring.  The report also recommended that monitoring at workplaces include not only personal
14    sampling and measurements inside the facility, but also measurements of nanomaterials in drains and in
15    the exhausted air to help ensure protection of the environment.
16          Finally, several companies are developing or have developed air monitoring devices for
17    nanoparticle  detection. The parameters that each device measures vary (Bennett, 2005; TRS
18    Environmental, 2009; van  den Brink, 2008).
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               Questions about  Characterizing Nanoscale
                                       Titanium  Dioxide
    1-1.  To evaluate nano-Ti02 (in these or other applications) or to compare products containing nano-Ti02, is further
         standardization or refinement of terminology needed? If so, is such an effort underway and/or what terminology is
         most important to standardize?

    1-2.  Have the properties of nano-Ti02 in different applications been adequately characterized?  If not, is the problem that
         methods are not generally available or that existing methods have not been widely applied? If new methods are
         needed, what properties should they measure?

    1-3.  Which coatings, dopings, carriers, dispersants, and emulsion types are most prevalent in different applications of
         nano-Ti02?

    1-4.  What are the potential implications (e.g., in terms of physical and chemical properties) of differences in the
         composition and mineralogy of different forms of nano-Ti02 (e.g., rutile and anatase)?

    1-5.  How do coatings applied for different purposes (e.g., to disperse particles or to decrease photocatalysis) interact or
         affect other properties of nano-Ti02?

    1-6.  What factors determine whether and to what extent aggregation or agglomeration of nano-Ti02 occurs?

    1-7.  Are data available that indicate the level of agglomeration/aggregation/dispersion of nano-Ti02 in specific products?  If
         so, what do the data show?

    1-8.  Is there a difference between the opacity of nano-Ti02 aggregates and conventional Ti02 particles of nominally similar
         size (e.g., because of light passing through pores in aggregates)? If so, what are the implications of such a
         difference?

    1-9.  Regarding the properties of aggregates and agglomerates and proper characterization of particle size, what insight is
         available from study of other nanoparticles?

   1-10.  What existing or emerging analytical techniques might be relevant or useful for material characterization? For
         example, could field flow fractionation (FFF) be used for characterization of particle size and elemental composition?

   1-11.  Do surface area measurements in air (e.g., BET analysis) correlate to surface area in an aqueous environment? If so,
         what is the extent of their accuracy and precision?
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                     Chapter  2.  Life Cycle  Stages

 1          This chapter discusses the life cycle of nanoscale titanium dioxide (nano-TiO2) as either a water
 2    treatment agent or an ingredient in topical sunscreen.  Each stage in the life cycles of the respective
 3    applications is considered from the standpoint of potential releases to the environment.


      2.1.  Feedstocks
 4          Two ores, ilmenite (FeTiO3) and rutile (TiO2), predominate as feedstock materials for TiO2
 5    production (nano and otherwise) (Haridasan et al., 2008).  Ilmenite and rutile are often found together, but
 6    ilmenite is found and mined in far greater quantities (at a ratio of more than 10:1 by weight) (Gambogi,
 7    2008) and supplies ~ 90% of titanium minerals worldwide. For rutile-based manufacturing processes, the
 8    most common manufacturing pathway for producing TiO2 of all kinds is via the chloride route using
 9    titanium tetrachloride (TiCl4), a liquid that accounts for about 60% of current manufacturing (Hext et al.,
10    2005). Creating synthetic rutile from ilmenite is often more economical than eliminating impurities from
11    natural rutile.
12          World ilmenite production in 2007 was around  5.6 million metric tons, and world rutile production
13    was around 0.5 million metric tons. The nations that produce the greatest quantities of ilmenite are
14    Australia, South Africa, Canada, China, Norway, India, the United States, and Ukraine. Significant
15    producers of rutile include Australia, Ukraine, South Africa, India, and the United States (Gambogi,
16    2008). An estimated 1 billion tons of TiO2 could be produced from existing world ilmenite resources,
17    with another 230 million tons from rutile deposits (Mineral Information Institute, 2009).
18          In the United States, ilmenite and rutile are extracted by surface mining or reprocessing of mine
19    tailings at two sites in Florida and Virginia. Combined ilmenite and rutile production is approximately
20    0.3 million metric tons. Mine and mill employment at these sites was estimated at 229 persons in 2007,
21    down from 344 in 2003 (Gambogi, 2008).
22          Low levels of radioactive materials are present in ilmenite and natural rutile (Collier et al., 2001;
23    Haridasan et al., 2008). A study in India found that those who work with ilmenite could be exposed to an
24    annual dose of 1 millisievert (mSv) of gamma radiation and another 0.7 mSv of radioactivity via particle
25    inhalation, mostly due to thorium. Thorium radioactivity in ilmenite was about 60% of the regulatory
26    exemption limit established in the International Atomic Energy Agency (IAEA) Basic Safety Standards.
27    Levels of radioactivity in natural rutile, ilmenite-derived synthetic rutile, and TiO2 pigment (produced by


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 1    the chloride route, particle size not specified) are lower than ilmenite, while levels of radioactivity (from
 2    radium as well as thorium) in solid wastes and liquid effluent are elevated compared with ilmenite
 3    (Haridasanetal, 2008).
 4          Another common feedstock is titanium sulfate solution, which can be hydrolyzed to form TiO2.
 5    The sulfate method begins with ground ilmenite or titanium slag.
                             Questions about Feedstocks
       2.1 -1.  Are certain feedstocks more relevant to producing nano-Ti02 specifically for water treatment or sunscreen
             applications?
       2.1-2.  What contaminants, nanoscale and larger, might be released, and in what quantities, in relation to the procurement
             and processing of feedstocks for nano-Ti02?
      2.2.  Manufacturing
 6          Around 2005, annual global production of nano-TiO2 was estimated at 2000 metric tons, with an
 7    overall market value of $70 million (Dransfield, 2005; Osterwalder et al., 2006). About 65% of
 8    production was thought to have gone to "personal  care" applications such as topical sunscreens and
 9    cosmetics, with the remainder used in industrial applications such as plastics, catalysts, and ceramics.
10    Commercial production of nano-TiO2 for years 2006-2010 has been estimated at 5000 metric tons/year,
11    and more than 10,000 metric tons/year for years 2011-2014 (United Nations Environment Programme,
12    2007). Recently, Robichaud et al. (2009) estimated current and future worldwide production levels of
13    nano-TiO2 at considerably higher levels, with an upper estimate of approximately 2.5 million metric tons
14    by 2025. Thus far, nano-TiO2 production has represented a small fraction of overall TiO2 production,
15    which commanded a market of 4.5 million metric tons and $9 billion (Dransfield, 2005; Osterwalder et
16    al., 2006).
17          Manufacturers and researchers report nano-TiO2 synthesis by various techniques, including
18    chemical vapor deposition (CVD), flame hydrolysis, sol-gel, calcination, aerosol pyrolysis, and colloidal
19    synthesis (Wahi et al., 2006). CVD, commonly used for production of both conventional and nanoscale
20    TiO2, involves the conversion of a volatile compound to a nonvolatile solid that deposits on a substrate
21    (Li et al., 2003; Nagaveni et al., 2004).  A variety of techniques are used to generate the vapor and collect
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 1    the particles, including plasma, high temperatures, pressure, and injection, among others (Aitken et al.,
 2    2004).
 3          According to one industrial manufacturer of nanoscale titania, flame hydrolysis can generate high-
 4    purity nano-TiO2 using TiCl4 as a feedstock (Degussa, 2004). Like CVD, flame hydrolysis can be used to
 5    deposit a thin film on a surface, a process known as flame hydrolysis deposition (FHD). In FHD, an inert
 6    gas carries TiCl4 into a flame that produces hydrogen chloride and the metal oxide (Tok et al., 2009).
 7    Flame hydrolysis is used for manufacturing P25 and yields agglomerated particles with a mean diameter
 8    of about 3.6 (im, with the smallest 4% of particles having an average diameter of 160 nm (Klaessig,
 9    2006).
10          Anticipated by-products of the chloride method of TiO2 production include those resulting from
11    chlorine contamination of the TiO2 (from the TiCl4 precursor). Warheit et al. (2007b) have suggested that
12    solutions of P25 in water are acidic (pH = 3.28) because of chloride ions on the particle surface. Other
13    information, however, indicates that a steam washing step during the manufacturing process removes
14    hydrochloric acid adsorbed on the surface of P25 (Vormberg, 2004).
15          When photocatalytic or other applications require smaller particles, additional post-manufacturing
16    processes that are sufficiently energetic to break apart the aggregates/agglomerates might be used, with
17    surfactants or solvents used to help keep the particles apart after separation (Hewitt, 1996; Porter et al.,
18    2008). Also, nanoscale particles might be sonicated to increase dispersion (Bihari et al., 2008).
19          Another method of TiO2 production, which could be the preferred method of nano-TiO2 production
20    in commercial settings, is the sulfate process (Medley, 2008). Details on this and other processes used in
21    producing nano-TiO2 can be found in Appendix B.


      2.2.1.  Water Treatment
22          No information was found on processes specific to preparing or formulating nano-TiO2 for use in
23    drinking water treatment. P25 is used in a commercial water treatment system (Photo-Cat from Purifies)
24    that can be used for drinking water, ground water, and waste water treatment (NSF International, 2009;
25    Pichat, 2003;  Purifies Solutions, 2008);.  For this treatment system, P25 is neither specially prepared nor
26    coated (Powell, pers. comm., 2009).


      2.2.2.  Sunscreen
27           Unlike for water treatment agents, information on the manufacture of topical sunscreens that
28    incorporate nano-TiO2is relatively abundant.  Although specific details of manufacturing protocols are


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 1    typically proprietary, general information on manufacturing processes and materials is available. The
 2    choice of nano-TiO2 crystalline form is a key issue in manufacturing sunscreens because forms differ in
 3    photostability. In particular, rutile is much more photostable than anatase (Chaudhuri and Majewski,
 4    1998; Maynard, 2008). Although less photostable, anatase appears to be in common use:  Barker and
 5    Branch studied five TiO2 sunscreens purchased over the counter and found that one was pure rutile, while
 6    the other four were anatase/rutile mixes in which anatase predominated (Barker and Branch, 2008).
 7           To increase nano-TiO2 photostability, the particles are commonly given a surface coating such as
 8    silica, alumina, simethicone, or a variety of other compounds (see Appendix B for more information on
 9    coatings). Another technique for increasing photostability is "doping" nano-TiO2 particles by embedding
10    within them minute amounts of metals such as manganese, vanadium, chromium, and iron (Park et al,
11    2006).
12           Another important consideration in the manufacture of most topical sunscreens is the use of a
13    liquid medium, or dispersion, to ensure that nano-TiO2 will be distributed evenly, thereby reducing
14    aggregation and agglomeration (which could negatively impact ultraviolet (UV) scattering performance
15    and transparency by increasing the effective particle size). Sunscreen manufacturers can purchase nano-
16    TiO2 powder and formulate their own dispersion, or they can purchase ready-made "predispersions."
17           Surface coatings influence the interaction of nano-TiO2 with the dispersion medium, which can be
18    water-based (aqueous), oil-based, or silicone-based. These and many other factors figure into the
19    manufacture of sunscreens, including pH; emulsifiers; emollients; other physical UV blockers (e.g., ZnO,
20    which can also be micronized); chemical UV filters; and various  inert ingredients to achieve the desired
21    viscosity/liquidity, spray-ability, color/transparency, water resistance, and spreadability.  More detailed
22    information on manufacturing processes is presented in Appendix B.
                            Questions  about Manufacturing
       2.2-1.  How do various manufacturing processes for nano-Ti02 affect their physicochemical properties?
       2.2-2.  How are manufacturing processes likely to evolve with increasing demand for nano-Ti02?
       2.2-3.  Are certain manufacturing processes used specifically for nano-Ti02 as a water treatment agent or as topical
             sunscreen?
       2.2-4.  What waste products or other by-products, both nanoscale and larger, might be released, and in what quantities, for
             nano-Ti02 manufacturing processes?
       2.2-5.  Where is nano-Ti02 manufactured?  What is the potential for general population exposure to nano-Ti02 in these
             areas?
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      2.3.  Distribution and  Storage
 1          Limited information about nano-TiO2 distribution and storage was located.  P25 is shipped as a
 2    powder in 10-kilogram (kg) "multilayer ventilated paper bags, equipped with an additional polyethylene
 3    lining when required" (Degussa, 2007). Another brand of photocatalytic nano-TiO2 (KRONOS vlp 7000,
 4    7001, and 7500) is also shipped in 10-kg paper bags (KRONOS International, 2006).  Nano-TiO2 powders
 5    from Sigma, on the other hand, are shipped in amber glass bottles enclosed in foil  or plastic bags, which
 6    are shrink-wrapped before being placed in cardboard boxes with shipping cushion peanuts. P25
 7    presumably could be stored as a powder in a chemical storage facility in the original 10-kg shipping bags.
 8    Degussa recommends storing it in closed containers under dry conditions (Degussa, 2007). Releases
 9    could occur if bags were damaged during shipping or storage. Standard good management practices
10    would be expected to reduce the occurrence of accidental releases, but to what extent is unknown.
11          As a dispersion, nano-TiO2 is shipped in pails, drums, or totes (Klaessig, 2008). Sigma ships its
12    nano-TiO2 dispersion in essentially the same way nano-TiO2 powders are shipped.  Dispersion-formulated
13    nano-TiO2 presumably would require protection from freezing.  Depending on where  accidental releases
14    of such dispersions occurred, nano-TiO2 could be released into water or soil during shipment or
15    discharged into industrial or municipal waste water treatment systems during storage.

      2.3.1. Water Treatment
16          No information pertaining  specifically to the distribution  and storage of nano-TiO2 water treatment
17    agents was located.

      2.3.2. Sunscreen
18          Topical sunscreen products are generally packaged in retail-sized bottles and shipped in larger
19    containers to wholesalers, retailers, and direct marketers.  Little  information is available on methods of
20    shipping or storage. Consumers generally handle only retail-sized packages.
21          Industry data from the 1990s, although perhaps out of date, sheds light on the distribution chain of
22    sunscreens. Sales in supermarkets, drugstores, and mass merchandise outlets accounted for about two-
23    thirds of the total U.S. sun-care retail sales in 1992-1993, according to Davis (1993).  The remaining one-
24    third was attributed to sales in department stores and other "prestige" stores. Sun-care products are also
25    sold by direct marketers (e.g., Avon, Amway, Mary Kay), discount stores, swimwear stores, and small
26    variety stores (e.g., those  near beaches and ski slopes) (Davis, 1993).
27          At any point in the distribution-to-storage chain, accidental releases could occur. For example, a
28    shipping accident, a dropped palette, or crushed retail-size container(s) could lead  to releases.

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                    Questions  about Distribution and Storage
       2.3-1.  How is nano-Ti02 shipped (i.e., what are the relative frequencies for shipments in bulk, paper bags, or drums, or by
             truck or rail)? How far is it shipped? In what quantities?
       2.3-2.  Are data available or can they be collected or estimated for accident rates and routine product releases associated
             with various modes of shipping and storage? To what degree could best practices reduce such occurrences?
       2.3-3.  How is nano-Ti02 stored (e.g., in warehouses, sunscreen manufacturing plants, and water treatment facilities)?
       2.3-4.  Does the use of "ventilated paper bags" increase the possibility of accidental spillage during shipment and storage?
             Are any guidelines available on whether protective packaging (e.g., additional polyethylene lining) is warranted?
       2.3-5.  Could vermin breach storage containers and contribute to environmental releases or become part of an
             environmental exposure pathway?
       2.3-6.  Would prolonged storage in adverse or less than ideal climates (e.g., cold or humid environments) alter nano-Ti02
             characteristics and behavior?
       2.3-7.  How much nano-Ti02 could be released under various routine and accidental scenarios of distribution and storage?
      2.4.  Use

      2.4.1. Water Treatment
 1          Nano-TiO2 could be used in various ways to treat drinking water, as discussed in Section 1.5.1.
 2    This discussion, however, assumes that nano-TiO2 would be used in water treatment facilities only for
 3    removing arsenic.
 4          Roughly 54,000 community water systems  in the United States serve more than 95% of the
 5    population (U.S. EPA, 2006c). Most of these systems apply some form of treatment to remove  or
 6    neutralize chemical or microbial contaminants. Those that do not apply treatment serve less than 5% of
 7    the U.S. population; these systems are generally small or medium-sized (i.e., serving no more than 10,000
 8    people) and rely on ground water (U.S. EPA, 2002). Public water systems are required to keep  arsenic
 9    concentrations in delivered water at or below a maximum contaminant level (MCL) of 0.01 milligrams
10    per liter (mg/L) (U.S. EPA, 2006a). About 5% of community water systems in the United States (i.e.,
11    about 3,000 systems serving 11 million people) have taken some action to be in compliance with the
12    arsenic MCL (U.S. EPA, 2007a).  Likewise, about 5% of 20,000 non-transient non-community water
13    systems that serve at least 25 of the same people more than 6 months of the year, such as schools,

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 1    churches, nursing homes, and factories (i.e., about 1,100 systems serving 2 million people) have also
 2    taken some action to comply with the arsenic MCL (U.S. EPA, 2007a). Altogether, about 13 million
 3    people use water that is treated to remove arsenic.  Although it is unknown to what extent nano-TiO2
 4    might be used in any of these systems  in the future, these numbers provide perspective on its potential
 5    usage for drinking water treatment.
 6          Depending on the type of water treatment system, nano-TiO2 might be used as powder (e.g., in a
 7    slurry) or fixed on a supporting material.  Each approach has its potential advantages and disadvantages.
 8    Powdered nano-TiO2 has a large surface area and offers highly efficient photocatalytic oxidation, but a
 9    means to filter or recycle all of the photocatalyst is required (Dionysiou, pers. comm., 2009; Pichat,
10    2003). This suggests the possibility that some amount of nano-TiO2 suspended in water might pass
11    through filters, including microfilters.  Also, if nano-TiO2 builds up on the filter matrix (i.e., if it is not
12    removed by filter backwashing and hydraulic cleaning of sand), it could saturate the filtration medium,
13    and small quantities might be released with filtered water into subsequent steps of the treatment sequence.
14    Fixed nano-TiO2 has a smaller surface area and thus is less efficient. Although the attachment to the
15    supporting material should allow no leaching, a fixed photocatalyst might not require filters or recycling
16    systems to remove nano-TiO2 from the final product (Dionysiou, pers. comm., 2009).
17          Zhang et al. (2008) investigated the removal of nano-TiO2 in a simulated conventional water
18    treatment procedure, which included coagulation, flocculation, sedimentation, filtration, and disinfection.
19    Two types of nano-TiO2 (crystal form unspecified, primary particle sizes of 15 and 40 nm, and aggregates
20    200 and 500 nm, respectively) in 2-L jars were subjected to the treatment procedure. Adding magnesium
21    chloride (MgQ2) or alum (A12(SO4)3-16H2O), followed by coagulation, flocculation, and sedimentation,
22    still left more than 20% of an initial  10-mg/L concentration of nano-TiO2 in the settled water.
23    Furthermore, the removal efficiency was lower in tap water than in buffered nanopure water (pH 5.6) due
24    to the presence of organic matter in the tap water. Membrane  filtration with a pore size of 0.45 (im (450
25    nm) after sedimentation removed nano-TiO2 aggregates larger than 500 nm, leaving only 1-8% of the
26    initial TiO2 in the treated water. Although most,  but not all, of the nano-TiO2 in the initial water was
27    removed, this level of filtration is not typical in water treatment plants (Flummer, 2008; Kline, 2008), nor
28    is it available in most whole-house filtration systems (Johnson, 2005).
29          At least two commercially available water treatment systems can employ nano-TiO2, although to
30    date they are not known to be routinely used in this manner. One uses nano-TiO2 in a fixed membrane
31    and the other uses nano-TiO2 in a slurry. A system from Matrix Photocatalytic Inc. uses a tube covered
32    with fiberglass mesh in which nano-TiO2 is embedded; the tube contains water that circulates and
33    ultraviolet (UV) lamps illuminate the outside (Dionysiou, pers. comm., 2009; Pichat, 2003). In the Photo-
34    Cat system by Purifies, nano-TiO2 (P25) circulates in a slurry  inside a narrow annulus surrounded by a
35    UV lamp (Pichat, 2003).  A ceramic membrane filters out nano-TiO2 (Purifies Solutions, 2008). No

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 1    empirical data are available on the life expectancy of either system or whether they can release nano-TiO2
 2    into treated water.
 3          The Purifies system was pilot-tested for two months in a community drinking water treatment
 4    facility (Purifies Solutions, 2008). The ceramic membrane used to filter nano-TiO2 (particles as small as
 5    12 nm) from the finished product was reported to require no servicing or cleaning during the 2-month
 6    period because the nano-TiO2 particles collected in the membrane were removed by bursts of high-
 7    pressure air (Pichat, 2003; Purifies Solutions, 2008). Although the purpose of this pilot test was not to
 8    remove arsenic, several studies have bench-tested nano-TiO2 in slurry systems for removal of arsenic from
 9    water (Dutta et al., 2004; Ferguson et al., 2005; Lee and Choi, 2002; Li et al, 2003; Meridian Institute,
10    2006). Higher arsenic oxidation rates occurred using a slurry that was continuously stirred (compared to
11    immobilized nano-TiO2) (Li et al., 2003). In actual use, steps likely would be taken to keep nano-TiO2
12    dispersed during treatment, which could affect solubility and particle agglomeration.  Surface
13    modification could affect dispersion and could also improve the material's photocatalytic properties as
14    described (Ryu and Choi,  2004). Additionally, numerous chemicals can be added for drinking water
15    treatment (NSF International, 2007), any or some combination of which could affect the solubility,
16    particle size, and behavior of the nano-TiO2.


      2.4.2. Sunscreen
17          The estimated use of sunscreen can vary greatly among surveys, but it is clear that its use is
18    significant (Kasparian et al., 2009; Keeney et al., 2009). Four U.S. studies that collected data in the years
19    1995-1999, with 1,000 to more than 10,000 participants in each survey, showed that approximately one in
20    three people said they use sunscreen regularly (Cokkinides et al., 2001; Geller et al., 2002; Santmyire et
21    al., 2001; Weinstock et al., 2000). In three studies, 31-45% of survey respondents said they routinely or
22    often use sunscreen (Cokkinides et al., 2001; Geller et al., 2002; Weinstock et al., 2000). In another
23    study, 30% of respondents said they were very likely to use sunscreen when they were outdoors
24    (Santmyire et al., 2001). More recently, data from the 2005 Health Information National Trends  Survey
25    in the United States showed that among a total of 496 Latino participants, 15% reported that they always
26    use sunscreen, 9% reported often use of sunscreen, and 20% reported that they sometimes use sunscreen
27    (Andreeva et al., 2009). In a 2007 survey, the Skin Cancer Foundation and iVillage (2007) found that
28    11% of respondents use sunscreen with a sunburn protection factor (SPF) of 15 or higher "every  day" and
29    59% of respondents use sunscreen at least occasionally (up from 39% in a 2003 survey), where SPF is
30    defined by FDA (2009) as a "measure of how much solar energy (UV radiation) is required to produce
31    sunburn on protected skin (i.e., in the presence of sunscreen) relative to the amount of solar energy

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 1    required to produce sunburn on unprotected skin." Of those who wear sunscreen, 74% reapply it "at least
 2    every 4-6 hours or after swimming or sweating," and 28% reapply it every two hours, the Skin Cancer
 3    Foundation's recommended rate of reapplication (Skin Cancer Foundation, 2007).
 4          While the use of sunscreen may be lower in young adults and adolescents than adults (Kasparian et
 5    al., 2009), sunscreen use is likely to be higher in young children. Robinson et al. (2000) surveyed 503
 6    people in the summer of 1997, and found that 54% of parents reported that their child always or usually
 7    used a sunscreen, but only 27% of parents used sunscreen themselves during the previous weekend.  This
 8    is consistent with a survey of 254 parents in June-July of 1999 by Weinstein et al. (2001) in Chicago, in
 9    which parents reported more frequent use of sunscreen on their children than on themselves.
10          The total amount of sunscreen, and more particularly the total amount of nano-TiO2 in sunscreen,
11    used in the United States is unknown. Furthermore, the available survey data does not differentiate
12    between sunscreen products with or without nano-TiO2, although the percentage of sunscreen with nano-
13    TiO2 is thought to be substantial.  In 2006, the Australian  Therapeutic  Goods Administration (TGA)
14    estimated that 70% of sunscreens containing titanium and 30% of sunscreens containing zinc in Australia
15    were formulated with nanoparticles (TGA, 2006).
16          As noted in Section 2.2, annual global production of nano-TiO2 was estimated at 2000 metric tons
17    around 2005, with about 65%, or 1300 metric tons, used in "personal care" products such as topical
18    sunscreens and cosmetics (Dransfield, 2005; Osterwalder et al.,  2006).
19          A recent report by Barker and Branch (2008) has noted that the surface coatings on nano-TiO2 in
20    many sunscreens might not be stable or effective. The investigators studied the weathering of paint in
21    contact with sunscreen.  Of five nano-TiO2 sunscreens tested, four released photocatalytically generated
22    hydroxyl radicals that accelerated the weathering of the paint. All four of those sunscreens used an
23    anatase/rutile mix.  The one nano-TiO2 sunscreen formulation that showed no appreciable effect on paint
24    weathering used 100% rutile doped with  manganese rather than surface coating (Barker and Branch,
25    2008).
                                    Questions about Use
       2.4-1.  To what extent is nano-Ti02 used or could be used for either drinking water or waste water treatment? Are data
             available (e.g., volume of water currently treated in the United States for arsenic, amount of Ti02 needed to treat a
             given volume of water) that would permit an estimate of potential use?
       2.4-2.  Which water treatment processes use or would use nano-Ti02 and in what quantities? Would the type of process
             depend on the size of a treatment facility or the size of the population served, or both?
                                                                           Questions continued on next page.
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                                     Questions about Use
       2.4-3.  What percentage of the nano-Ti02 would settle out in floe or become part of the filter matrix? What percentage would
             be released into finished water? Are measurement or monitoring methods adequate to detect such particles?
       2.4-4.  Water distribution systems often have substantial biofilm or corrosion development, despite the implementation of
             control practices. Would the presence of nano-Ti02 influence the bacterial biofilm community or the occurrence of
             corrosion?
       2.4-5.  What is the total quantity of nano-Ti02 used in topical sunscreen products in the United States and worldwide?
       2.4-6.  What is the maximum quantity and frequency of personal sunscreen use in relation to season, geographic location,
             demographics, and other variables?
       2.4-7.  How much nano-Ti02 enters the environment under different scenarios and conditions of sunscreen use (e.g.,
             ambient air and water temperature, swimming, bathing)? Under what conditions would nano-Ti02 be released from
             the sunscreen matrix?
      2.5. Disposal
      2.5.1. Water Treatment
 1          Most community water treatment filters, with regular backwashing, have an indefinite life span.
 2    Slow sand filters are generally cleaned not by backwashing, but by scraping and replacing the top layer of
 3    sand. Scraped sand is normally cleaned hydraulically and stockpiled for later reuse (Cleasby and
 4    Logsdon, 1999). This process creates waste water, which might be recycled in the treatment train or
 5    discharged (e.g., to a municipal sewer).  Eventually, the filter sand or other filter materials would need to
 6    be disposed of.
 7          After nano-TiO2 is used in water treatment, a sludge material (floe) containing nano-TiO2 would
 8    likely be created. In one scenario, the sludge might be taken to a landfill. Whether TiO2 could diffuse
 9    (and thus be released) from a solid matrix such as sludge is unknown.  Nano-TiO2 and other contaminants
10    such as residual arsenic could become suspended in leachate and enter ground water, or they could pass
11    through a solid waste facility liner into the subsurface.
12          Under a different scenario,  the sludge could be used for land application. In this case, the sludge
13    would undergo some treatment, which is generally required for removing pathogenic organisms and
14    regulated contaminants  such as lead and arsenic [titanium is not regulated in biosolids under U.S. EPA's
15    Biosolids Rule, Part 503; see (U.S. EPA, 1994)]. Such treatment might include high temperature or high
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 1    pH processing (U.S. EPA, 1994). The treated sludge then could be applied to land for agricultural use,
 2    reclamation sites, golf courses, public parks, and other areas where nutrient-rich organic matter is useful,
 3    including forests, parks, roadsides, and in some cases, residences (U.S. EPA, 1994). Roughly half of
 4    treated sewage sludge is applied to land, and less than 1% of all U.S. agricultural land uses treated sewage
 5    sludge (U.S. EPA, 2006b).
 6          If nano-TiO2 is present in finished drinking water that reaches the tap, it would eventually enter the
 7    ambient environment or be captured by a waste water stream, after which it could enter sewage treatment
 8    facilities.

      2.5.2. Sunscreen

 9          Sunscreen containers likely would be disposed of primarily as municipal solid waste and thus end
10    up in landfills or incinerators.  The potential for leaching of nano-TiO2 from landfill disposal of containers
11    would depend on many factors, including the integrity of liners and leachate collection systems, if
12    present. Incineration of sunscreen containers raises the question of whether nano-TiO2 could enter the
13    stack and be released to air, or become  a trace contaminant in fly or bottom ash.
14          Depending on the packaging, sunscreen containers might be recycled, suggesting the possibility
15    that nano-TiO2 could be incorporated into recycled materials.
                                 Questions about Disposal
       2.5-1.  How much residual nano-Ti02 is present in packaging of the primary material or derived products? How is such
             packaging disposed of?
       2.5-2.  If nano-Ti02 were to become much more widely used and produced at a much higher volume, would packaging and
             shipping methods of nano-Ti02 change? If so, how would such change affect the potential release and exposure
             during transport, storage, and disposal?
       2.5-3.  In water treatment, how are filter materials and associated waste/waste water containing nano-Ti02 disposed of or
             recycled?
       2.5-4.  How are large quantities of sunscreen (e.g., sub-par batches rejected during manufacturing) handled?
       2.5-5.  How much nano-Ti02 is present in sunscreen containers that are discarded?  Are there any circumstances where
             such discarded product could enter a microenvironment at significant levels?
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                    Chapter 3.  Fate and Transport

 1          Chapter 3 explores what might happen to nanoscale titanium dioxide (nano-TiO2) after it is
 2    released to the environment at various stages of the product life cycles for water treatment agents or
 3    topical sunscreens. Nano-TiO2 could be released to air, water, or soil and then transported or transformed
 4    through chemical or biological processes. The lack of data on the fate and transport of nano-TiO2 by-
 5    products and waste produced during the manufacturing process precludes a comprehensive discussion in
 6    this chapter.  This chapter does, however, summarize what is known about the environmental pathways
 7    and transport and transformation processes of nano-TiO2 related to the various life-cycle stages described
 8    in Chapter 2.
 9          Although most studies cited in this chapter consider nano-TiO2 in aggregate or agglomerate form
10    (as discussed in Chapter 1), whether all constituent primary particles remain in clusters if conditions
11    change is unclear. Disaggregation, for example, can occur at certain pHpzc levels. ThepHpzcofa
12    nanoparticle  is defined as the pH at the "point of zero charge," which occurs when the net electric charge
13    at the particle surface is zero.  At the pHpzc particles fail to electrostatically repel each other.  In laboratory
14    studies, the size range of aggregates and the presence of free nano-TiO2 particles (ranging from 5 to 50
15    nm in size) were found to be pH-dependent: when the solution pH differed from the pHpzc of the
16    particles, the aggregates tended to be smaller (Dunphy Guzman, pers. comm., 2007; Dunphy Guzman et
17    al., 2006). Sampled aggregates ranged up tolSO nm in size, and contained an estimated 8 to 4,000
18    nanoparticles (Dunphy Guzman et al., 2006). The pHpzc also depends at least in part on the crystallinity
19    of the nano-TiO2 particles: Finnegan et al.  (2007) reported pHpzc values of ~5.9 for rutile and ~6.3 for
20    anatase.
21          The pHpzc depends in part on the crystal form of the nano-TiO2 particles. Finnegan et al. (2007)
22    reported pHpzc values of-5.9 for rutile and  -6.3 for anatase.  The degree of aggregation generally
23    increases with the presence of salt or increases in ionic strength, minerals, and organic matter in water
24    (Domingos et al., 2009a; French et al., 2009).
25          Despite the presence, and sometimes the predominance, of large particles, several researchers
26    investigating laboratory-synthesized and commercial nano-TiO2 products have found free particles or
27    aggregates with diameters less than 100 nm in varying amounts, depending on synthesis method,
28    temperature,  solution pH, and the presence  of buffers (Kormann et al., 1988; Li et al., 2003; Nagaveni et
29    al., 2004; Pena  et al., 2006; Ryu and Choi, 2006; Sun et al., 2007; Wahi et al., 2006). Moreover, some
30    preparations  are specifically designed to generate dispersed particles (e.g., Seok et al., 2006) to increase
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 1    the efficacy of nano-TiO2 as a catalyst, increasing the potential for the presence of disaggregated nano-
 2    TiO2 to occur in the environment. However, no studies of nano-TiO2 aggregation/disaggregation behavior
 3    under "real-world" ambient environmental conditions, irrespective of medium, were located.
      3.1.  Water
 4          Although numerous studies characterize nano-TiO2 particles in aqueous solution under laboratory
 5    conditions, the fate and behavior of the particles in the environment have received less attention. One
 6    report indicates that nano-TiO2 was detected in river water in Montana, but the source (natural or
 7    engineered) and the concentration of nano-TiO2 were not determined (Wigginton et al., 2007).
 8          Several physicochemical properties of nano-TiO2 can contribute directly to its environmental fate
 9    and transport in water.  Long et al. (2006) reported that P25 rapidly aggregated in both Hank's Basic Salt
10    Solution (HBSS) and Dulbecco's Modified Eagle's Medium (DMEM) buffer solutions, both of which are
11    high-osmolarity fluids that contain high concentrations of the monovalent cations Na+ and K+ [160
12    millimolar (mM)] and the divalent cations Ca2+ and Mg2+ (2 mM). The ionic strengths of these two
13    solutions are approximately 155 mM and 166 mM, respectively. After 1 minute of sonication,
14    aggregation continued for 20-45 minutes until a steady-state, stable aggregate size formed. The steady-
15    state aggregate sizes ranged from 826 to 2,368 nm and the concentration of P25  ranged from 2.5 to 120
16    parts per million (ppm).
17          Ridley et al. (2006) found that results were  reproducible for classical titration procedures (with
18    modification) to characterize the surface charging properties of a commercially available, uncoated
19    anatase nano-TiO2 product (from Ishihara Techno  Corporation, Osaka, Japan) in suspension. These
20    findings demonstrate that water treatment pH can  affect the surface charging properties, and thus the
21    aggregation/agglomeration, potential bioavailability, and reactivity of nano-TiO2.
22          Schmidt and Vogelsberger (2006) studied the solubility of four types of nano-TiO2 (P25 from
23    Degussa, DT5 ID and G5 from Millennium Chemicals, and an original formulation - presumably all
24    uncoated particles) in various aqueous solutions, particularly focusing on the kinetics of the dissolution
25    process. At the beginning of the process, solubility increased rapidly over time and then reached a steady-
26    state value. The maximum solubility value (i.e., saturation concentration) was observed to depend on the
27    morphology of the TiO2, the crystalline form of the nano-TiO2, and on the size of the nanoparticles
28    exposed to dissolution. The saturation concentrations were higher in hydrolysis-generated nano-TiO2
29    than in precipitation-generated nano-TiO2, and higher in smaller particles than larger particles.
30          Sager et al. (2007b)  attempted to disperse nano-TiO2, and other types of nano-sized particles in
31    several suspension media, including phosphate-buffered saline (PBS), rat and mouse bronchoalveolar

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 1    lavage fluid (BALF), and dipalmitoyl phosphatidylcholine (DPPC). Although PBS was not a satisfactory
 2    medium, BALF was an excellent medium for dispersing the particles.  The dispersion was also
 3    unsatisfactory in saline containing albumin alone or DPPC alone at concentrations found in BALF.
 4    Combinations of protein and DPPC were satisfactory, but slightly less effective, substitutes for BALF.
 5    These findings demonstrate the importance of the suspension media, but they are not necessarily relevant
 6    to natural aquatic conditions.
 7          Although many studies have demonstrated the potential to use nano-TiO2 for waste water treatment
 8    (Chen and Ray, 2001; Han et al., 2009; Khataee et al, 2009; Rincon and Pulgarin, 2003; Wang et al.,
 9    2008c; Watlington, 2005; Xu et al., 2009b), data on the fate of nano-TiO2 in waste water treatment are
10    scarce. Westerhoff et al., (2008) however, have reported the occurrence of nano-TiO2 at full-scale waste
11    water treatment plants (in both raw and finished waters) in a conference proceeding abstract. The authors
12    predicted nominal nanomaterial concentrations on the order of one part per billion in liquid discharges
13    from waste water treatment systems, with higher concentrations in waste water biosolids (which may
14    subsequently be applied to land, landfilled, or incinerated).
15          Other types of nanoparticles also have been studied in waste water treatment plants. Limbach et al.
16    (2008) studied the fate of cerium oxide nanoparticles (20-50 run diameter) in a model waste water
17    treatment plant under a variety of conditions  (e.g., with different surfactants to stabilize dispersions, and
18    in media with different ionic strengths and pH values). They found that surfactants stabilized dispersions
19    under a wide range of test pH values even at  high ionic strength. The model sewage treatment  plant
20    consistently reduced the cerium oxide nanoparticle concentration in the waste water from 100 ppm to 2-5
21    ppm.  Most nanoparticles were removed via agglomeration with microorganisms in the sedimentation
22    sludge.  Comparing the physical properties and behavior of various oxides, the  investigators speculated
23    that TiO2 and other insoluble oxides would behave similarly to cerium oxide, while more soluble or
24    reactive oxides like zinc oxide (ZnO) would be even more likely to aggregate and be more amenable to
25    removal by sedimentation. The investigators cautioned, however, that the high nanoparticle concentration
26    (100 ppm) used in the study favors aggregation, and that at more realistic initial concentrations, a greater
27    percentage  of nanoparticles are likely to break through.
28          Although no field studies on the behavior of nano-TiO2 in the environment were  identified, that
29    conventional TiO2 can photogenerate fairly long-lived reactive oxygen species such as hydrogen peroxide
30    in aqueous  environments has long been recognized (Harbour et al., 1985).  Similar behavior would be
31    anticipated for nano-TiO2.
32          The interaction between nano-TiO2 and natural organic matter, which is ubiquitous in the
33    environment, has been investigated in controlled conditions in the laboratory. Yang et al. (2009) found
34    that humic acid, a common type of natural organic matter, is easily adsorbed onto nano-TiO2 in aqueous

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 1    media (Yang et al., 2009). Because humic acid adsorption decreased the £, (Chi) potential (i.e., increased
 2    electrostatic repulsion) of nano-TiO2 particles, humic acid-coated nano-TiO2 could be more easily
 3    dispersed and suspended and thus more stable in an aqueous medium than uncoated nano-TiO2 (Yang et
 4    al., 2009).
      3.1.1. Drinking Water Treatment-specific
 5          Although the processes for using nano-TiO2 for commercial water treatment are not yet well
 6    established and therefore a definitive understanding of nano-TiO2 fate is not possible, nano-TiO2 is not
 7    expected to be destroyed.  One might anticipate that, given the size of nano-TiO2, it would remain
 8    suspended in solution; alternatively, it could adsorb to other particles and become part of the
 9    sedimentation (floe).  Some evidence suggests that nano-TiO2 suspended in water could pass though
10    various stages of conventional treatment and filtration, perhaps even microfiltration (Zhang et al., 2007).
11          Various fate pathway scenarios could be anticipated for nano-TiO2 post-treatment. For example,
12    nano-TiO2 might remain in solution as colloidal particles in the water and enter water tanks or reservoirs.
13    If some water were lost from the distribution system via leaks or spills, nano-TiO2 could end up in surface
14    waters or the subsurface environment. If nano-TiO2 were to enter ground water aquifers, nano-TiO2
15    would presumably persist as a particle, given that other inorganic compounds are not readily broken down
16    in that environment; however, particle size and other characteristics could change. Conceivably, nano-
17    TiO2 could release, or modify the bioavailability of, other water contaminants of concern.
18          In another scenario, nano-TiO2 might settle with floe in the sedimentation step, where it
19    presumably could become part of the sediment sludge and be partially removed from the water with the
20    sludge (AWWA, 2003). The discarded sediment could be transported off-site for disposal or reuse. For
21    example, sludge could be  used as cover in municipal solid waste landfills or applied to agricultural or
22    recreational land.
23          Alternatively, nano-TiO2 might become part of the filter matrix.  Conventional water treatment
24    processes apply filtration following flocculation and sedimentation. U.S. EPA's Filter Backwash
25    Recycling Rule (U.S. EPA, 2001) requires that, when the filter is backwashed, the  water be recycled back
26    into the coagulation process. This could reintroduce nano-TiO2 into the treatment process, but the
27    implications for levels of nano-TiO2 in finished water are not clear.
28          If nano-TiO2 is present in the final drinking water product that reaches the tap, it eventually might
29    enter the ambient environment or be captured by a waste water stream, after which it could reach a waste
30    water treatment plant. If the particular waste water treatment method employed does not remove nano-
31    TiO2, it is likely to enter downstream water sources.

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      3.1.2. Sunscreen-Specific
 1          The environmental fate of nano-TiO2 in topical sunscreens could be affected by the surface
 2    treatments and doping applied to nano-TiO2 particles, by the sunscreen vehicle, or by any number of other
 3    constituents in such products (see Appendix B). Nano-TiO2 in emulsion, dispersion, and possibly
 4    powdered form could be present in waste water (e.g., from equipment and site cleaning) and solid waste
 5    from sunscreen manufacturing facilities, depending on the trapping and filtration processes the facility
 6    uses.  In the powdered form, nano-TiO2 could escape the facility through air venting and filtration
 7    systems.
 8          Nano-TiO2 also could be released to natural water bodies or waste water through bathing or laundry
 9    following sunscreen use. Swimming in artificial pools could result in an accumulation of sunscreen
10    material in the water and potential release into the environment as untreated waste water. If nano-TiO2
11    remains mobile in water, it could enter downstream water sources in a manner similar to that of the  nano-
12    TiO2 used for drinking water treatment.
13          Parallels are suggested by recent studies that have detected topical sunscreen constituents in
14    untreated waste water, treated waste water, surface water (lakes and rivers), fish from lakes and rivers,
15    and biosolids (Balmer et al., 2005; Pent et al., 2008; Rodil and Moeder, 2008).  The organic compounds
16    detected in these studies were UV filter compounds such as 4-MBC (4-methylbenzylidene camphor) and
17    OC (octocrylene), which generally biodegrade slowly and can bioaccumulate. Some evidence also
18    indicates that nano-TiO2 can bioaccumulate (Zhang et al., 2006). Although nano-TiO2 is unlikely to
19    behave exactly the same way as other components of sunscreen, the observed nano-TiO2 bioaccumulation
20    in fish (Zhang et al., 2006) suggests the possibility of persistent presence of nano-TiO2.  However, no
21    studies to date have documented the occurrence of nano-TiO2 specifically from sunscreens in waste water
22    or natural water bodies.
      3.2.  Soil
23          Three studies were located that address the fate and transport of nano-TiO2 in soil. Dunphy
24    Guzman et al. (2006) studied the effect of pH on nano-TiO2 mobility in a model soil column. They found
25    that both surface potential and aggregate size influence transport. In the pH region where electrostatic
26    forces between nano-TiO2 aggregates and the experimental Pyrex surface should have been strong (pH
27    2.5 to 5.9), nano-TiO2 was highly mobile. The calculated interaction energy was expected to be greatest
28    for the largest aggregates at pH  12, but these were the particles that most strongly attached to
29    microchannel surfaces. At pH 3, where conditions were predicted to be favorable for negative/positive

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 1    interaction, 84% of the particles were transported. The authors concluded that current transport theory
 2    does not adequately predict nanoparticle and aggregated nanoparticle transport.  The results suggest that
 3    nano-TiO2 particles and aggregates of nanoparticles in a stable dispersion might be highly mobile in the
 4    subsurface over a wide range of conditions. This also raises the possibility that colloid transport
 5    mechanisms might be more relevant than particle transport.
 6          Lecoanet et al. (2004) showed that the mobility of aqueous anatase nano-TiO2 particles in a porous
 7    medium was comparable to that of other types of nanoparticles when compared on the basis of particle
 8    size. Primary particles of 40-nm diameter were found to be aggregated to a diameter of 198 nm. About
 9    55% was recovered after three pore volumes passed through the column, roughly twice the quantity of
10    ferroxane particles with mean diameter of 303 nm and just more than half the quantity of silica particles
11    with a diameter of 57 nm. After three pore volumes, approximately 95% of the 57-nm silica particles
12    were recovered, compared with 60% of the 135-nm silica particles.  Although the results are specific to
13    the experimental protocol, they suggest that particle size affects mobility of nanoparticles and that anatase
14    might be mobile in ground water (Lecoanet et al., 2004).
15          A recent study using soil samples from 11 sites found that nano-TiO2 could remain suspended in
16    soil suspensions for 10 days (Fang et al., 2009). Furthermore, the calculated maximum travel distance for
17    some soil samples was more than 30 cm, which suggested that nano-TiO2 might be transferred to deep
18    soil layers or even to ground water.  In general, large soil particles and low ionic strength conditions favor
19    nano-TiO2 movement, while high clay content, dissolved organic carbon, and salinity conditions favor
20    soil retention of nano-TiO2.
21          If nano-TiO2 enters municipal sewage systems, the plants would separate liquid waste from solid
22    waste and nano-TiO2 would likely be present in both waste streams. The solid waste, or sludge, could
23    present a route by which nano-TiO2 could enter soil media, and could be dealt with in a number of ways.
24    In one scenario, the sludge might be sent for land disposal. The ability of TiO2 to diffuse (and thus  be
25    released) from a solid matrix such as sludge is unknown. Nano-TiO2 and other contaminants such as
26    residual arsenic could become suspended in leachate and enter ground water, or they could pass through a
27    solid waste facility liner into the subsurface.
28          Under a different scenario, the sludge could be used for land application.  In this case, the sludge
29    would undergo some type of treatment, generally to remove pathogenic organisms and regulated
30    contaminants such as lead and arsenic [titanium is not regulated under U.S. EPA's Biosolids Rule, Part
31    503; see (U.S. EPA, 1994)]. The treatment might include high temperature or high pH processing, or both
32    (U.S. EPA, 1994).  The treated sludge could then be applied to land for agricultural use, reclamation sites,
33    golf courses, public parks, and other areas where nutrient-rich organic matter is useful, including forests,
34    parks, roadsides, and in some cases, residences (U.S. EPA, 1994). Roughly 50% of treated sludge is

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 1    applied to land, and less than 1% of all U.S. agricultural land uses treated sewage sludge (U.S. EPA,
 2    2006b).
 3          Nano-TiO2 in sludge could be broadly distributed to land used for crops or grazing, where it could
 4    enter the food chain, or to high-use areas such as parks, where people and pets could contact nano-TiO2 in
 5    soil or inhale wind-blown material. The nanomaterial could enter runoff and storm water during wet
 6    weather events, returning to the aquatic medium. Ecological receptors also could also be exposed to
 7    nano-TiO2 in soil by direct contact with soils or via the food web, including uptake by plants. Because it
 8    is an inorganic compound, nano-TiO2 in soil could be expected to persist, in the same way that
 9    conventional TiO2 is very thermodynamically stable and is unlikely to undergo significant transformation
10    in the environment. Reactivity of nano-sized TiO2, however, might differ (and is largely unknown at this
11    time) due to its greater surface area-to-volume ratio.
      3.2.1. Drinking Water Treatment-specific
12          One scenario by which nano-TiO2 specifically used in drinking water treatment could enter soils
13    would be through land application of sludge.  In addition to the sludge produced in waste water treatment
14    described above, a sludge material (floe) containing nano-TiO2 would likely be created in the process of
15    using nano-TiO2 to treat drinking water.  If nano-TiO2 settles with floe in the sedimentation step, it would
16    likely become part of the sediment sludge.  Similarly, as described above, if nano-TiO2 is present in
17    finished drinking water, it will eventually enter sewage treatment facilities where any residual nano-TiO2
18    could also enter the sediment sludge. The discarded sediment would be transported off-site and could be
19    used as cover in a municipal solid waste landfill or used for land application. Either use would result in
20    direct application of nano-TiO2-contaminated waste to soils. Alternatively, nano-TiO2 could enter soils if
21    treated water were used to irrigate residential or agricultural plants.  These scenarios could have
22    implications for soil microbes and could also be noteworthy in relation to uptake by edible vegetation.
      3.2.2. Sunscreen-specific
23          As described above, nano-TiO2 in topical sunscreens could end up in the sludge produced at a
24    waste water treatment plant. The disposal of this sludge on land seems likely to represent the primary
25    pathway by which nano-TiO2 in sunscreen could enter soil.
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      3.3.  Air
 1          Nano-TiO2 manufacturing facilities could emit such particles to the ambient atmosphere. An
 2    occupational exposure study by Berges et al. found that "outside the plant," the airborne TiO2 particle
 3    concentration was approximately 13,000 particles per cubic meter, with nearly 94% of particles 100 nm or
 4    less in size, and approximately 52% at 40-60 nm (Berges, 2007, 2008).
 5          Some potential for environmental or occupational atmospheric emissions and releases of nano-TiO2
 6    presumably exists if the transport or storage containers were to be compromised (e.g., due to a forklift
 7    error, train derailment, or truck accident). Also, land application of sludge from either drinking-water or
 8    waste-water treatment might also contribute nano-TiO2 to the atmosphere if dried material were to be re-
 9    entrained.
10          The large surface area of nano-TiO2 presents an opportunity for other co-occurring contaminants to
11    adsorb onto the surface, potentially changing the physicochemistry of the particle and the behavior and
12    effects of the other contaminant(s).  Such interactions have been well documented for particulate matter
13    and gasses (U.S. EPA, 2004).
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                      Questions  about  Fate  and  Transport
   3-1.   What are the relative contributions of different stages of the life cycles of water treatment and sunscreen products to
         environmental levels of nano-Ti02 and associated contaminants in air, water, and soil?

   3-2.   How do specific physicochemical properties, including particle surface treatments and aggregation/agglomeration,
         affect the fate and transport of nano-Ti02 in various environmental media?

   3-3.   Are available fate and transport models applicable to nano-Ti02? If not, can they be adapted, or are new models
         required?

   3-4.   Is information on environmental fate and transport of other substances available that might provide insights applicable
         to nano-Ti02?

   3-5.   If nano-Ti02 production were to increase greatly, the packing and transport methods are likely to be changed as well.
         How would this affect the fate and transport of nano-Ti02?

   3-6.   How might nano-Ti02 affect the fate and transport of metals and other potentially toxic substances in water or other
         environmental media?

   3-7.   What is the bioavailability of nano-Ti02 in land-applied sludge to both terrestrial and aquatic organisms? Is
         bioavailability likely to change when nano-Ti02 is incorporated into sludge and is allowed to "age" (in-situ weathering)?

   3-8.   What effect, if any, do coatings,  dopings, carriers, dispersants, and emulsion types have on biopersistence and
         bioaccumulation?

   3-9.   Can the photocatalytic properties of nano-Ti02 cause other unintended substances to form, for example, degradation
         products, in various environmental media?

  3-10.   Will nano-Ti02 affect the efficacy of other major elements of water treatment processes (e.g., chemical disinfection,
         the coagulant concentration necessary for effective organics removal)?

  3-11.   What influence could other drinking water contaminants, including arsenic, have on the chemical properties or
         behavior of nano-Ti02?

  3-12.   Irradiated photocatalytic nano-Ti02 is potentially biocidal and antimicrobial.  What is the potential for interactions of
         nano-Ti02 with microbes needed in water treatment systems?

  3-13.   What are the key environmental factors (e.g., pH, natural organic matter type and concentration, temperature) that
         facilitate or hinder nano-Ti02 stability in the aqueous environment? Would humic acids or other common constituents
         or contaminants in water undergoing treatment affect the fate, including agglomeration/aggregation properties, of
         Ti02?

  3-14.   What is the impact to nutrient and metals cycling and microbial diversity when sludge with nano-Ti02 is applied to
         soils?

  3-15.   How do sunscreen ingredients affect nano-Ti02 fate and transport?

                                                                                 Questions continued on next page.
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                   Questions about  Fate and Transport
  3-16. Can agglomeration/disagglomeration in the environment be predicted on the basis of physical properties of the
       particle, for example, size, shape, or coating?

  3-17. What is the likelihood that nano-Ti02 in biosolids will become part of the food web and ground water contamination?

  3-18. What is the potential for plant uptake of nano-Ti02 from contaminated soil and irrigation water?
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                       Chapter 4.  Exposure-Dose

                                  Characterization

 1          This chapter examines the potential for biota and humans to be exposed to nanoscale titanium
 2    dioxide (nano-TiO2) and associated pollutants through various environmental pathways tracing back to
 3    the life cycle of two types of applications of nano-TiO2, water treatment agents and topical sunscreens.
 4    Exposure is more than the occurrence of a substance in the environment; actual contact between the
 5    substance and an organism must occur.  Exposure characterization entails much more than simply
 6    identifying the concentration of a substance in the environment. It also involves, for example, various
 7    temporal and spatial dimensions, including activity patterns and other complex variables. For nano-TiO2,
 8    even characterizing the primary material of interest, as discussed in Chapter 1, is not a simple matter.
 9    Further complications arise when considering the potential for aggregate exposure across multiple routes
10    (e.g., inhalation, ingestion, dermal absorption) and for cumulative exposure to multiple contaminants that
11    derive, either directly or indirectly, from the life cycle of the products in question.
12          Dose4 refers to the amount of a substance that enters an organism by crossing a biological barrier
13    such as the skin, the respiratory tract, the gastrointestinal tract, or the eyes. Dose can vary for individuals
14    exposed to the same concentration of a substance. For example, an adult and a child in a room breathing
15    the same air containing a contaminant would both inhale the same  contaminant concentration, but the
16    inhaled contaminant quantity and absorbed dose would differ due to differences in physiology (e.g.,
17    respiration rates), morphology (e.g., lung volume and surface area), and other variables such as clearance.
18    Dose can also reflect the integration of aggregate exposures across different routes of uptake.
19          Organisms might be exposed to nano-TiO2 in the environment at any stage of the product life cycle.
20    In the feedstock and manufacturing process, nano-TiO2 could be present in the air exhaust, waste-water
21    effluent, and solid waste, if appropriate control technologies are not in use. Nano-TiO2 in the air can lead
22    to inhalation exposure to organisms in the area.  The material could agglomerate or attach to other
23    pollutants and deposit on soil and water surfaces, as well as on animals, whose grooming habits could
24    then result in ingestion of nanomaterials. Nano-TiO2 in soil could become airborne when the soil is dry
25    and windblown, or leak into water bodies when the soil is saturated with water.
      4 The distinction between exposure and dose in this document is consistent with risk assessment usage.  In
      toxicology, however, the term dose is often used to refer to the amount of a substance given to test subjects, as well
      as the amount that enters the subjects.  Applied, external, and potential dose (e.g., on the skin, in the lung or
      digestive tract) in toxicology roughly equate to exposure in risk assessment; absorbed dose (amount entering the
      circulation) and target organ dose (amount taken up by a specific organ) in toxicology roughly equate to dose in risk
      assessment.
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 1          During distribution and storage, nano-TiO2 could be released accidentally into the environment,
 2    and cleaning the contaminated site with water could lead to nano-TiO2 exposure to both aquatic and
 3    terrestrial organisms. The use of nano-TiO2 in drinking water treatment could result in some level of
 4    nano-TiO2 in water, as described in Chapter 3, and thus potential exposure to human populations as well
 5    as biota. The use of sunscreens containing nano-TiO2 is expected to lead to nano-TiO2 in waste water
 6    after users bathe or shower to remove residual sunscreen on the skin and launder clothes containing traces
 7    of sunscreen. Because typical waste water treatment plants currently do not monitor for or specifically
 8    target nanomaterials, nano-TiO2 might not be completely removed by treatment. Therefore, nano-TiO2
 9    might be present in the effluent and lead to exposure to aquatic species.  In the disposal stage, wastes
10    from factories and research facilities containing nanomaterials are often incinerated, possibly releasing
11    nano-TiO2 into the air. Household wastes containing consumer products with nano-TiO2 might be
12    incinerated or landfilled; landfilling might lead to nano-TiO2 leaching into ground water.
13          Occupational exposure to nano-TiO2 and associated contaminants (e.g., waste by-products) could
14    occur even with appropriate safety and protective practices. (See Appendix C for a more thorough
15    discussion of occupational exposure control measures.) Such occupational exposures could differ from
16    those of the general public in various ways. For example, workers might more likely be exposed to free
17    nano-TiO2, whereas the public might more commonly encounter nano-TiO2 embedded in a product.
18    Exposure durations and concentrations are also likely to be different in occupational settings. Likewise,
19    dose levels could differ between workers and the general population or even between workers in different
20    occupations, depending on factors such as respiration rates in relation to sedentary or strenuous activity in
21    the presence of airborne nano-TiO2.


      4.1. Aggregate Exposure to Nano-TiOz  from  Multiple
        Sources and  Pathways

22         Nano-TiO2 is used in various products, raising the possibility that biota and humans could be
23    exposed to nano-TiO2 from more than  one source. Such sources might include water treatment agents,
24    topical sunscreens, cosmeceuticals (traditional cosmetics such as moisturizers and color cosmetics that
25    incorporate active sunscreen ingredients with nano-TiO2), sun-protective clothing, cleaning agents, air
26    purifiers, coatings, and food packaging, among many others (Woodrow Wilson International Center for
27    Scholars, 2006). Kaegi et al. (2008), for example, reported nano-TiO2 in water runoff from both new and
28    naturally aged building facades painted with paint containing nano-TiO2. Hsu and Chein (2007) found
29    that nano-TiO2 powder-coated materials (wood, polymer, and tiles) under various conditions emitted
30    nanoparticles to the air. Of course, merely the presence of nano-TiO2 in a product does not mean that
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 1    exposure will occur.  For example, if nano-TiO2 is firmly embedded in a product and the product remains
 2    intact, little or no exposure to nano-TiO2 might actually occur.
 3          A hypothetical scenario for aggregate exposure to nano-TiO2 in both water and sunscreen could
 4    involve a person's ingesting the water (oral route), bathing (dermal) or showering (dermal and inhalation)
 5    in it, applying sunscreen lotion to the skin (dermal), ingestion of sunscreen through hand-to-mouth
 6    contact (oral), or uptake from hand-to-eye (ocular) contact. The latter two exposures pathways are
 7    particularly relevant for young children. Biota also could be subject to aggregate exposures. A fish, for
 8    example, could take up nano-TiO2 that originated from a waste water treatment facility and could also
 9    ingest prey whose contamination originated from ambient water, sediment, or other biota containing
10    sunscreen constituents. The seemingly widespread occurrence of nanoparticles of various types in aquatic
11    media reported by Wigginton et al. (2007) lends plausibility to these scenarios.


      4.2. Cumulative Exposure to Nano-TiOz and Other
        Contaminants

12          Nano-TiO2 is not the only substance relative to the life cycle of products containing nano-TiO2 to
13    which biota and humans could be exposed. As noted in Chapter 2, releases of other contaminants might
14    also occur during various stages of the product life cycle, particularly waste materials during feedstock
15    processing and during manufacturing of the primary product. Such waste materials are not necessarily
16    nanoscale in size. As described in Chapter 3, if wastes are released into the environment, they could
17    undergo transformation, potentially resulting in even more types of contaminants; they might also be
18    transported to other locations, e.g., downstream or downwind.
19          The creation of secondary contaminants through transformation processes in various environmental
20    media also raises the possibility of exposure to substances  indirectly related to nano-TiO2. Many
21    nanoparticles, including nano-TiO2, tend to bind transitional metals and organic chemical pollutants
22    (Nagaveni et al., 2004; Pena et al., 2006). With a tendency to adsorb pollutants and an ability to penetrate
23    the body and cells (see sections 4.6.1 Inhalation, 4.6.3 Ingestion, and 4.6.4 Blood-Brain Barrier and
24    Placental Transfer), nano-TiO2 could carry toxic pollutants to sites where the pollutants would not
25    normally go (Moore, 2006).  This type of "Trojan horse" effect could result in increased uptake of other
26    pollutants or interactive effects that would otherwise not occur if these substances were only present
27    individually.
28          Increased uptake of other pollutants in the presence of nano-TiO2 has been reported by Sun et al.
29    (2007) and Zhang et al. (2007; 2006) (see Table 4-1). Sun et al. (2007) demonstrated that arsenic as
30    arsenate [As(V)] strongly binds to AEROXIDE® P25 (P25) in water and that carp exposed to water
31    containing 10 milligrams per liter (mg/L) of this photocatalytic nano-TiO2 and 200 micrograms per liter

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             arsenate accumulated more arsenic than fish exposed to either nano-TiO2 or arsenic alone.  The
 2    bioconcentration factor of arsenic5 was more than twice as high when nano-TiO2 was present than when it
 3    was not (Sun et al, 2007). The tested arsenate concentration, 200 (ig/L, is environmentally relevant,
 4    given that higher total arsenic concentrations (mainly inorganic arsenic in the forms of arsenite and
 5    arsenate) in drinking water have been reported in many countries, including Bangladesh, China, Chile,
 6    and India (Basu et al., 2004; Feng et al., 2001; Moore et al., 1997; Tian et al., 2001). The presence of
 7    nano-TiO2 did not alter the distribution of arsenic within fish tissues. Over various time intervals, arsenic
 8    and TiO2 accumulated significantly in the intestine, stomach, and gills, and to a lesser degree in liver,
 9    skin, and scales; the least accumulation occurred in muscle. Because the accumulation of arsenic  was
10    much greater in the presence of nano-TiO2, Sun et al. (2007) concluded that adsorption to nano-TiO2
11    facilitated arsenic transport and uptake.
Table 4-1 . Tissue concentrations of various pollutants in fish after exposures to nano-TiCh in
water.
Test Species
Fish (carp, Cyprinus
carpio)
Fish (carp, Cyprinus
carpio)
Fish (carp, Cyprinus
carpio)
Fish (rainbow trout,
Oncorhynchus
mykiss)
Material
21 -nm primary
particle, 50- to 200-
nm aggregates in
water (P25)
(photocatalytic)
21 -nm primary
particle, 40- to 500-
nm aggregates in
water (P25)
(photocatalytic)
21 -nm primary
particle, BET 50
m2/g (P25)
(photocatalytic)
21-nm,75% rutile:
25% anatase,
sonicated (P25)
(photocatalytic)
Protocol
(no UV illumination,
unless specified)
Up to 25-day exposure to
3 and 10 mg/L nano-Ti02
(water changed daily, Ti02
concentrations in water ~2
and ~7 mg/L, respectively,
after the first few hours)
Up to 25-day exposure to
10 mg/L nano-Ti02with and
without 200 pg/L arsenate
Up to 25-day exposure to
-97 ng/L cadmium alone,
cadmium with 10 mg/L nano-
Ti02, or cadmium with
10 mg/L natural sediment
particles
0-, 7-, or 1 4-day exposure to
0,0.1,0.5, or 1.0 mg/L nano-
Ti02
Study Outcome
Ti02 accumulated in internal organs > gills > skin and
scales > muscle
Bioconcentration factors were higher at 3 mg/L than
at 10 mg/L
Arsenate adsorbed onto nano-Ti02
Higher arsenic concentrations in tissues (skin and
scales; muscle; gills; liver; stomach; intestine) with
arsenate plus nano-TiCh exposure, compared to
arsenate exposure alone
Cadmium adsorbed onto nano-Ti02
Higher cadmium concentrations in tissues (skin and
scale; muscle; gills; viscera; whole body) with
cadmium plus nano-Ti02 exposure, compared to
cadmium exposure alone, or cadmium plus natural
sediment particles
No clear treatment or time-dependent effects on Ti
levels in gill, liver, or muscle. In brain, a transient but
statistically significant decrease in Ti concentrations
compared to initial fish, but no exposure
concentration-effect.
Respiratory distress, organ pathologies, and
oxidative stress at concentrations as low as 0.1 mg/L.
Reference
Zhang etal. (2006)
Sun etal. (2007)
Zhang etal. (2007)
Federici etal.
(2007)
BET - Brunauer, Emmett, Teller method of calculating surface area
P25-AEROXIDE®P25
      5 The bioconcentration factor of arsenic = 1000 x arsenic concentration in fish (ug/g dry weight) / arsenic
       concentration in water (ug/L).
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 1          Zhang et al. (2007) showed that nano-TiO2 (21 nm) also enhanced cadmium uptake in carp. After
 2    20 days of exposure, the bioconcentration factor for whole-body cadmium was 64.4 in carp exposed to
 3    cadmium alone, but reached 606 in carp exposed to both cadmium and nano-TiO2. Natural sediment
 4    particles (19 (im) did not increase  cadmium uptake.  Both nano-TiO2 and sediment particles adsorb
 5    cadmium and reach equilibrium within 30 minutes, but nano-TiO2 adsorbed more than 5 times as much
 6    cadmium as the sediment particles. Based on the facts that nano-TiO2 can adsorb cadmium and that
 7    concentrations of cadmium and nano-TiO2 are positively correlated, the authors suggested that increased
 8    cadmium uptake in the presence of nano-TiO2 may have been due to accumulation of cadmium adsorbed
 9    on nano-TiO2 (i.e., facilitated transport).
10          Zhang et al. (2007) also found that carp exposed to cadmium in water (at approximately 97 (ig/L)
11    along with 10 mg/L photocatalytic nano-TiO2 accumulated more cadmium than fish exposed to either
12    nano-TiO2 or cadmium alone (Table 4-1).  After 25 days of exposure, cadmium concentration in the whole
13    fish was 9.07 (ig/g in the cadmium-only group and 22.3 (ig/g in the cadmium-plus-nano-TiO2 group,
14    indicating a 146% increase in the cadmium bioconcentration factor in the presence of nano-TiO2. When
15    carp were analyzed after 20 days of exposure, cadmium concentrations in all groups were higher in
16    internal organs than in gills, muscle, and skin and scale (Zhang et al., 2007). Unlike nano-TiO2, natural
17    sediment particles (at equivalent concentrations) did not affect cadmium bioaccumulation.  The authors
18    also reported a positive correlation between nano-TiO2 concentration and cadmium concentration in the
19    carp, and found high nano-TiO2 concentrations in the gills. The increases in cadmium bioaccumulation
20    could be due to increased transport of cadmium into carp via adsorption to nano-TiO2. The transport
21    routes could be from water onto the gill surfaces or from consumed food into internal organs. Toxicity
22    was not measured in this study.
23          The fact that organic disinfection by-products can be formed by the photocatalytic oxidation of
24    conventional TiO2 in treating drinking water (Richardson et al.,  1996)  suggests the possibility that nano-
25    TiO2 could have the same effect. Richardson et al. (1996) compared the organic disinfection by-products
26    detected after using (1) chlorine as the sole disinfectant and (2) TiO2/ultraviolet (UV) light treatment
27    followed by chlorination. The authors reported  detecting an additional by-product (tentatively identified
28    as dihyro-4,5-dichloro-2(3H)furanone) after the combined TiO2/UV and chlorine treatment compared to
29    chlorine treatment alone. Overall, however, the numbers and concentrations of chlorinated disinfection
30    by-products were lower after combined TiO2/UV and chlorine treatment than after chlorination alone.
31          Cumulative exposure to nanomaterials could also occur.  Some consumer products contain more
32    than one type of nanomaterials, e.g., nano-TiO2  and nano-silver have been used together in multiple
33    products  (The Project on Emerging Nanotechnologies, 2009).
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      4.3. Models to  Estimate Exposure
 1          The U.S. Environmental Protection Agency (U.S. EPA) uses various models to estimate exposures
 2    for chemical assessments, some of which are described on the Web sites for the Council for Regulatory
 3    Environmental Modeling (U.S. EPA, 2009b) and the Center for Exposure Assessment Modeling (U.S.
 4    EPA, 2009a).  For example, the Exposure and Fate Assessment Screening Tool Version 2.0 (E-FAST
 5    V2.0) is a publicly available program EPA uses for screening-level assessments of conventional industrial
 6    chemicals. The tool provides estimates of aquatic exposure, general population exposure, and consumer
 7    exposure based on release data (U.S. EPA, 2007b). Other fate and transport models also might be
 8    relevant, for example, the Particle Tracking Model (PTM) the Army Corps of Engineers developed
 9    (Demirbilek, 2005). However, these models were not developed for nanomaterials and have not been
10    tested for their ability to estimate nanomaterial exposures, although they perhaps could be used or adapted
11    for qualitative exposure estimation in lieu of quantitative release data.
12          Although empirical data on nano-TiO2 concentrations in the environment are currently lacking, a
13    recent study used computer modeling to predict nano-TiO2 concentrations in different environmental
14    media. Using limited data from published literature and various assumptions, researchers in Switzerland
15    developed models to estimate predicted environmental concentrations (PEC) and predicted no-effect
16    concentrations (PNEC).  PEC values were calculated for "realistic exposure scenarios" (based on nano-
17    TiO2 use, estimated as 25 tons per year in Switzerland) and for "high exposure scenarios" (based on
18    500 tons per year). The authors estimated that more than 60% of nano-TiO2 is used in cosmetics,
19    including sunscreen, and that most of it is discharged into wastewater. To estimate PNEC, the lowest no-
20    observed-effect concentration  [based on a published study on acute toxicity to Daphnia by Hund-Rinke
21    and Simon (2006)] was divided by an assessment factor of 1000, in accordance with the Technical
22    Guidance Document on Risk Assessment published by European Chemicals Bureau, because, as the
23    authors noted, the "accuracy of the data was low"  (European Chemicals Bureau, 2003;  Mueller and
24    Nowack, 2008). The PEC  of nano-TiO2 in water was 0.7 ug/L ("realistic scenario") or  16 (ig/L ("high
25    scenario"), compared to a PNEC of <1 (ig/L (for daphnia). The authors (Mueller and Nowack, 2008)
26    stated that, given that the PEC is close to or greater than the PNEC, European Union authorities would
27    consider the substance "of concern" and call for more data to validate the result (Umwelt Bundes Amt,
28    2009).


      4.4.  Biota

29          Various scenarios and ways in which nano-TiO2 from water treatment agents and topical sunscreens
30    could enter different environmental media were described in Chapters 2 and 3. In this section, some of


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 1    these scenarios are explored further in relation to exposure of aquatic and terrestrial organisms to nano-
 2    TiO2 under various conditions.  Also discussed are the potential for bioaccumulation and entry of nano-
 3    TiO2 into the food web.
      4.4.1. Aquatic
 4          Data on sediment concentrations of nano-TiO2, whether in a laboratory or a natural environment,
 5    are limited. Nano-TiO2 concentrations could be higher at the sediment surface than in the water (Handy
 6    et al., 2008b).  Settling of nano-TiO2 aggregates (with nano-TiO2 or with organic matter) would increase
 7    nano-TiO2 exposure to benthic and benthopelagic species, such as mussels, sea cucumbers, marine
 8    worms, flatfish, and other species that sometimes feed at the bottom of water bodies. At the same time,
 9    settling decreases nano-TiO2 concentrations in the water column and would be expected to decrease
10    exposure to suspension feeders (such as Daphnid) and animals that live in or drink the water.
11          Nanoparticles can also deposit or aggregate on the surfaces of aquatic organisms. Surface
12    aggregation can be caused by the slower flow near the interface between liquids  and solids or by the
13    viscous properties of the surface of an organism (Handy et al., 2008b).  Surface deposition or aggregation
14    can result in a higher concentration of nano-TiO2 on the organism's surface than  in the water, and might
15    cause toxicity even if the nano-TiO2 does not enter the cells.  Surf ace-acting metal toxicity of nano-TiO2
16    has been suggested as a cause of gill damage in rainbow trout where the titanium concentration in gill
17    tissue was not increased (Federici et al., 2007).
18          Because water flow is also slower near the interface with air, higher concentrations of nanoparticles
19    are also expected at the air-water interface.  Consequently, organisms living at the water surface, such as
20    zooplankton (microscopic invertebrates that float or swim in water), phytoplankton (primarily single-
21    celled algae),  and eggs of aquatic and amphibian species at the water surface, could be exposed to higher
22    nanoparticle concentrations than organisms living throughout the water column.

      4.4.1.1. Bioaccumulation

23          Zhang et al. (2006) found that nano-TiO2 can accumulate internally in carp (Table 4-1).  The
24    authors exposed carp to photocatalytic nano-TiO2, or P25 for up to 25 days. Before dissection and TiO2
25    analysis, carp were rinsed and wiped.  The nominal concentrations of nano-TiO2 in the water were 3 and
26    10 mg/L (based on the amount of stock nano-TiO2 suspension added to the fish tank), and the authors
27    reported that nano-TiO2 concentrations were 2 and 7 mg/L after 24 hours, with most of the decreases
28    occurring within 4 hours after the addition of stock solution.  The TiO2 concentration in carp tissue
29    increased rapidly over the first 10 days and then more gradually between day 10 and day 25. TiO2
30    concentrations were highest in visceral organs, distantly followed by gills, and then closely followed by

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 1    skin and scales (one sample), and muscle.  The bioconcentration factors in the visceral organs were
 2    approximately 2100 at 3 mg/L, and approximately 1400 at 10 mg/L.
 3          In contrast to the finding of bioaccumulation of nano-TiO2 in carp that Zhang et al. (2006)
 4    reported, Federici et al. (2007) detected no accumulation in trout exposed to up to 1 mg/L nano-TiO2 for
 5    14 days. Although the findings appear contradictory, each study might simply reflect the results of the
 6    specific test conditions. For instance, the rainbow trout were exposed to lower concentrations of nano-
 7    TiO2 than were the carp. The Federici et al. (2007) study used photocatalytic nano-TiO2 (P25),  and 80%
 8    of the water in the fish tank was changed every 12 hours. Similar to Zhang et al. (2006), Federici et al.
 9    (2007) reported that more than 85% of the  initial nano-TiO2 concentrations in the tank water remained
10    after 12 hours. Other environmental factors, such as water temperature at 14 °C for trout and at 23 °C for
11    carp, could influence the behavior or effects of nano-TiO2 and contribute to the difference between these
12    two studies. Furthermore, carp feed mainly by grubbing in sediments, and therefore also could be
13    exposed to  settled nano-TiO2 aggregates, to which rainbow trout might not be exposed.
14          Although nano-TiO2 can bioaccumulate in fish, the uptake mechanism is not clear. Substances in
15    water can enter fish through waterborne exposure (through gills and then into blood through absorption),
16    dietary uptake, or cutaneous absorption.  Handy et al. (2008a) suggested that the  absorption of nano-TiO2
17    on the gill surface into the blood might be slow or uncertain, but that nano-TiO2 on the gut  surface might
18    be taken into cells by endocytosis.  Although intact fish skin is unlikely to be permeable to  nano-TiO2,
19    these authors proposed that cutaneous uptake of nano-TiO2 might be possible if the skin is infected or
20    inflamed (Handy et al., 2008a). Handy et al. (2008a) did not provide experimental data to support nano-
21    TiO2 uptake through endocytosis, but a recent in vitro study indicated that an endocytosis inhibitor,
22    Nystatin, decreased the mutation frequencies induced by exposures to 5-nm and 40-nm nano-TiO2, but
23    not 325-nm TiO2, in mouse embryo fibroblasts, implying that endocytosis is involved in modulating cellar
24    response to nano-TiO2 exposure (Xu et al., 2009a). The concentration of nano-TiO2 or Ti in cells was not
25    measured (Xu et al., 2009a).

      4.4.2. Terrestrial

26          Terrestrial organisms could be exposed to nano-TiO2 under various scenarios. For example,
27    spillage during shipping or storage, including breaching of containers by vermin, could result in contact
28    by microbial, invertebrate, and vertebrate species. Plants could be exposed by taking up water containing
29    nano-TiO2 or by growing  in soil that contains nano-TiO2, for example, as a result of application of sludge
30    from water treatment facilities. No empirical data on the potential for such exposures to terrestrial
31    organisms have been located.
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      4.4.3. Food Web

 1          Nano-TiO2 could enter the food web at various levels, depending on the point and extent of its
 2    release to the environment. If nano-TiO2 were dispersed in water, for example, it could be taken up by
 3    algae, which are primary producers in ecosystems.  Many invertebrates, which are primary consumers in
 4    aquatic systems, eat algae and in turn are consumed by larger animals such as fish. A common aquatic
 5    invertebrate is the water flea (genus Daphnia), which is a small crustacean filter feeder (also known as
 6    suspension feeder). Daphnids use their legs to generate water flow and use the comb-like setae on their
 7    thoracic limbs to strain or catch smaller organisms (such as algae) for consumption. Because daphnids
 8    have been reported to filter up to 120-160 mL each per day (Vanoverbeke, 2008), they could be exposed
 9    to quite high numbers of nanoparticles in water (Griffitt et al., 2008). Even if nano-TiO2 is not absorbed
10    into tissues, nano-TiO2 in the digestive tract of daphnids could still contribute to bioaccumulation in the
11    food web.


      4.5.  Humans

12          As noted at the beginning of this chapter, exposure is a complex function of not only the amount of
13    a substance in the environment but also various temporal and spatial dimensions of contact with the
14    substance. At this early stage of investigation and understanding of human exposure to nano-TiO2,
15    however, even basic information on the potential for and amount of human contact with this material is
16    limited. Moreover, exposure characterization encompasses not just the primary material but the
17    secondary waste and transformation products related to the entire life cycle of nano-TiO2 in various
18    applications. These indirect and secondary aspects of exposure are even less well understood and
19    therefore not discussed here. Their potential significance, however, should not be discounted.
20          The potential for human exposure to nano-TiO2 depends first on the production and use of this
21    material in the applications under consideration here. Generally, exposure related to life-cycle stages
22    leading up to actual use appears more likely to occur in occupational situations, whereas exposure related
23    to the use and disposal stages of the life cycle could occur in either occupational or non-occupational
24    settings. Although not absolute, this distinction provides a basis for discussing exposure with reference to
25    either the general population or the occupational population, both of which are essential in examining the
26    broad implications of nano-TiO2 in water treatment and topical sunscreens.
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      4.5.1. General  Population

      4.5.1.1. Water Treatment-specific

 1          Although the actual use of nano-TiO2 in water treatment facilities appears to be limited at present
 2    to pilot testing (see Section 2.4), the potential for general population exposure to nano-TiO2 if it were to
 3    be used widely could involve sizeable numbers of people, given the number of U.S. community water
 4    suppliers that currently treat drinking water to reduce arsenic levels. As discussed in Section 2.4.1, such
 5    water suppliers serve  roughly 13 million people in the United States alone.
 6          If nano-TiO2 were present in potable water, exposure could involve more than just ingesting the
 7    water.  Such water could be used for bathing, including showering, which could imply exposure not only
 8    by dermal contact but by inhalation of water droplets and even contact through the eyes. Also, the general
 9    population includes infants and other individuals who could have relatively greater exposure to water and
10    thus possible vulnerability if the water were contaminated.  For example, on a body weight basis,  1- to 3-
11    month-old infants consume far more water directly and indirectly than 18- to 21-year olds. The 90th
12    percentile consumption rate is 151 milliliters per kilogram per day (mL/kg/day) for these infants versus
13    17 mL/kg/day for the older age group [see Table 3-9 in (U.S. EPA, 2008a)]. Children also have a greater
14    water intake while swimming, so they may be more vulnerable to contaminated water in that respect as
15    well (U.S. EPA, 2008a).

      4.5.1.2. Sunscreen-specific

16          As discussed in Section 2.4.2, survey data from 2007 suggest that sunscreen might be used on a
17    daily basis by 33 million people in the United  States and on an occasional basis  by another 177 million.
18    Moreover, sunscreen use appears to be increasing.  According to the Skin Cancer Foundation (2007), the
19    percentage of people who use sunscreen at least occasionally rose from 39% to 59% between 2003 and
20    2007. Sunscreen use  is presumably greatest during the warmer months of the year, in warmer climates, or
21    during outdoor recreational activities at various times during the year. No information was found
22    regarding the proportion of use associated with water recreation and other specific venues or activities.
23          Topical sunscreens are available as traditional lotions, in spray-on form, and as wipes (Jeffries,
24    2007). Nano-TiO2 sunscreen powders are also available, according to the Woodrow  Wilson Center's
25    nanotechnology consumer product inventory (Woodrow Wilson International Center for Scholars, 2006).
26    Another sun protection option available to consumers is "cosmeceuticals," cosmetics that incorporate
27    active sunscreen ingredients (Davis, 1994). In the mid-1990s, up to 30% of lipsticks and 20% of makeup
28    were estimated to have sunburn protection factor (SPF) ratings, sunscreen claims, or both (Davis,  1994).
29    Other products with active sunscreen ingredients include hair care products (e.g., hair spray, gel, mousse,
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 1    and conditioner), alpha-hydroxy skin treatments, nail polish, and bath products.  Sun-protective clothing
 2    is also available (Davis, 1994).
 3          For the general population, the principal exposure route to nano-TiO2 in sunscreen is through the
 4    skin.  When sunscreen is applied by spray, inhalation presents another route, although it is not clear that
 5    the primary nanoparticles as such would be inhaled. Ingestion is also conceivable through hand-to-mouth
 6    contact and mucociliary clearance of inhaled nano-TiO2.
      Dermal  Exposure
 7          Potential nano-TiO2 dermal exposure from sunscreen use can be estimated by the amount of
 8    applied sunscreen. Although the recommended sunscreen application rate is 2 milligrams per square
 9    centimeter (mg/cm2) of skin (roughly 1.5 ounces or 3 tablespoons for the entire body of an average adult),
10    most consumers use 0.5 to 1.5 mg/cm2 skin (Srinivas et al., 2006). Assuming  sunscreen is applied to all
11    areas  of skin exposed to sun on a day at the beach or exposed to water while swimming, an adult would
12    use an estimated 10-46 g sunscreen per application, and a 3-year old would use an estimated 3-15 g
13    sunscreen per application (Table 4-2). Assuming that a sunscreen contains 5% nano-TiO2 (the mass
14    percent concentrations of nano-TiO2 in sunscreens range from 2% to 15%, see Table A-l in Appendix A),
15    the amounts of nano-TiO2 applied on the  skin could range from 0.5 to 2.3 g per person per application for
16    an adult, and 0.17 to 0.76 g per person per application for a 3-year old (Table 4-2).  Sunscreens, including
17    the water-resistant or water-proof types, should be reapplied every 2 hours, regardless of the SPF values.
18    Exposure to nano-TiO2 from sunscreen could range from 1.0 to 4.6 g for an adult and 0.33 to 1.5 g for a
19    3-year old for a half day at the beach (2 applications in 4 hours). As shown in Table 4-2, the ranges of
20    applied nano-TiO2 would be 12-55 mg per kg of body weight per application for a 3-year old and 8.0-37
21    mg per kg of body weight per application for an adult. This relatively higher exposure in young children
22    could be noteworthy in relation to indications that the skin of infants and young children might have less
23    barrier function than matured skin (Hostynek, 2003), although this contrasts with another report
24    indicating that human skin is mature both structurally and functionally at 2-3 weeks of age (Makri  et al.,
25    2004). Although not everyone applies sunscreen at the recommended dose and frequency in real life,
26    parents reported greater use of sunscreen on their children than on themselves (Weinstein et al., 2001).
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Table 4-2. Estimated dermal exposure to nano-TiOz from sunscreen
for adults and 3-year-old children. a

„ .. . Surface area of
Subject , . h , ,,



3-year-old child, total body surface - - .„
(50th percentile)


3-year-old child, total body surface -, - .„
(95th percentile)

Adult, body surface area subjected
to water contact in swimming 20,000
(50th percentile)
Adult, body surface area subjected
to water contact in swimming 23,000
(95th percentile)
Applied
sunscreen
surface
density
(mg/cm2)
0.5
1.5
2
0.5
1.5
2
0.5
1.5
2
0.5
1.5
2
Applied
sunscreen
amount
(mg/person/
application)
3,320
9,960
13,280
3,820
11,460
15,280
10,000
30,000
40,000
11,500
34,500
46,000
containing 5% nano-TiCh

Applied nano-Ti02
(mg/person/
application)

166
498
664
191
573
764
500
1,500
2,000
575
1,725
2,300

Applied nano-Ti02
(mg/kgBWc/
application)

12.0
35.9
47.9
13.8
41.3
55.1
8.0
24.0
32.1
9.2
27.6
36.9
       BW - Body weight
       a Actual concentrations of nano-Ti02 in commercial sunscreen on the market vary, with the high at nearly 15%. (See Table A-1 in Appendix A.)
       b Body surface area values are based on Tables 6-6 and 6-16 of U.S. EPA (1997).
       c The body weights used in the calculation were 14kg, the median for 36-month old females (CDC, 2000), and 62 kg, the median for adults 18-74 years old
       [Table 7.5 of U.S. EPA (1997)].
      Inhalation Exposure
 1          Consumers could inhale water aerosol  while showering or from nebulizing room humidifiers.
 2    Spray sunscreen  products also present an inhalation exposure scenario.  For such products and for water
 3    containing nano-TiO2, the characteristics of the resulting aerosol have not been documented in the
 4    published literature.  Section 4.5.2 discusses inhalation exposure to nano-TiO2 for several occupational
 5    scenarios.
      Oral Exposure
 6          Nano-TiO2 from sunscreen could be ingested by accident or as a result of routine hand-to-mouth
 7    contact (from residual sunscreen on hands), particularly for young children. If nano-TiO2 were inhaled,
 8    mucociliary clearance could lead to uptake through the gastrointestinal tract. Although no estimates of
 9    this type of nano-TiO2 exposure are available, dietary intake of all sizes of TiO2 from all sources (food,
10    pharmaceuticals, etc.) has been estimated.  The estimation was based on 7-day food diaries and records of
11    pharmaceutical, dietary supplement, and toothpaste use of 182 people in the United Kingdom.  The
12    amounts of TiO2  were calculated or estimated from product labels (the listing  of food-additive  TiO2 is
13    required by British law in most foods), manufacturer reports, and laboratory testing. The total  median
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 1    dietary intake of nano-TiO2 and micro-TiO2 (0.1-3 (im) was estimated to be 2.5 mg per individual per day
 2    (Lomer et al., 2004).  Food was the main source of dietary TiO2, followed by pharmaceuticals, dietary
 3    supplements, and toothpaste.  Individual TiO2 intake varied widely (0-112 mg per individual per day),
 4    and no particle size information was provided.
      4.5.2. Occupational
 5          Nearly every stage of the life cycle for the applications considered here presents some potential for
 6    occupational exposure to nano-TiO2.  Moreover, no exposure route can be ruled irrelevant to these
 7    workers. Thus, assessing occupational exposure is essential to completing a CEA of nano-TiO2 in either
 8    water treatment agents or topical sunscreens. As a frame of reference, NIOSH (2005) proposed a draft
 9    occupational exposure limit of 1.5 milligrams per cubic meter (mg/m3) for fine TiO2 (less than 2.5 (im in
10    size) and 0.1 mg/m3 for ultrafine TiO2 (less than 0.1 (im [100 nm]).
11          Most information on workplace TiO2 exposure relates to the production of conventional TiO2, not
12    nano-TiO2 specifically. Additionally, given that nano-TiO2 tends to agglomerate or aggregate,
13    occupational exposure conditions for nano-TiO2 could involve both nanoscale and larger than nanoscale
14    TiO2 particles.  The manufacturing stage of the life cycle comprises multiple processes that might vary in
15    exposure characteristics.  An epidemiologic study conducted in four U.S. TiO2 manufacturing factories
16    indicated that occupational exposure to TiO2 is greatest during bagging, milling/micronizing, and internal
17    recycling (shoveling spilled material from the floor into the processing bins) stages (Fryzek et al., 2003).
18          The manufacturer of P25 has stated on its Web site that workplace inhalation exposures to TiO2 are
19    typically less than 0.5 mg/m3 (Degussa, 2007).  The Web site also indicated that photocatalytic P25
20    production occurs in a closed reactor, which presumably limits exposure. The highest exposures the
21    manufacturer reported were less than 0.5 mg/m3 and occurred during the packaging step, which is also
22    enclosed.  This manufacturer is said to require the use of personal protective equipment during any repair
23    work that could lead to dust exposure (Maier, 2007).  Such information suggests limited potential for
24    inhalation exposure during P25 manufacturing,  but it does not address other routes such as dermal
25    exposure or incidental ingestion from hand-to-mouth contact.
26          Another manufacturer of nano-TiO2 products reported that air concentrations in production areas
27    for DuPont™ Light Stabilizer 210 and 220 (which protects plastic from UV damage) were less than
28    2 mg/m3, and in most cases were lower than the detection limit of 0.3 mg/m3 (size not specified) (DuPont,
29    2007). No exposure data were available for the material incorporation, packing, and product fabrication
30    areas. Although occupational exposure was stated to be low (DuPont, 2007), the  detection limit
31    (0.3 mg/m3) is above the draft NIOSH recommended limit for ultrafine or nano-TiO2, 0.1 mg/m3 (NIOSH,
32    2005).
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 1          Preliminary estimates of workplace exposure in a factory that produces rutile nano-TiO2 for
 2    sunscreen and cosmetics were reported by Berges (2007, 2008). Measurements were made in 2006, and
 3    then in 2007, when improvements to local exhaust systems were in operation (Berges, 2007, 2008). In
 4    2007, the TiO2 in the "inhalable" dust mass concentration at the bin filling station was 0.014 mg/m3, and
 5    the TiO2 in the "respirable" dust mass concentration was 0.004 mg/m3. [Inhalable refers to all particles
 6    that can enter the respiratory tract through the nose or mouth (e.g., up to about 100 (im); respirable refers
 7    to particles that penetrate to the alveolar (pulmonary) region with a mass median aerosol diameter
 8    (MMAD) of about 4 \am (European Committee for Standardization, 1993).]  In the bag filling area in
 9    2007, the TiO2 inhalable fraction was 0.028 mg/m3, and the respirable fraction was 0.022-0.042 mg/m3.
10    Personal sampling in 2007 over a 4.87-hour period measured 0.010 mg/m3 TiO2 in the respirable fraction.
11          Liao et al. (2009) further reported and analyzed the Berges (2007, 2008) data, as well as data from
12    several other sources to model the occupational exposure and characterize risk. In the bin filling area of
13    the facility studied by Berges (2007, 2008), the total airborne TiO2 particle number concentrations ranged
14    from 15,000 to  156,000 particles/cm3, with a measured size range of 14-673 nm. More than 97% of the
15    particles were 100 nm or less in size, and 60% were 20-30 nm. After a leak was sealed, the high-end
16    concentration decreased to less than 29,000 particles/cm3. Near the leak, the particle surface area
17    concentrations reached 200 square micrometers per cubic centimeters (|im2/cm3) for "alveolar deposited"
18    particles and 50 (im2/cm3 for "tracheobronchial deposited" particles. Under normal operating conditions,
19    the particle surface area concentrations were 50 (im2/cm3 for the alveolar deposited particles and
20    13 (im2/cm3 for the tracheobronchial deposited particles. Outside the plant, the airborne TiO2 particle
21    concentration was approximately 13,000 particles/cm3. Among other things, their model indicated that
22    the highest TiO2 burdens in terms of lung surface area of packers were 0.174 m2 (anatase) and 0.122 m2
23    (rutile) for particles  sized 10-20 nm.  For particle sizes 80-300 nm, the burdens were 0.002 m2 (anatase)
24    and 0.0017 m2 (rutile).  So-called surface treatment workers (involved in drying, packing, and blending
25    operations) had a higher TiO2 burden in the lung surface area. For particles 10-20 nm, the burdens were
26    0.40 m2 (anatase) and 0.28 m2 (rutile).
27          Using exposure data specific to particle size in the workplace from the Berges (2007, 2008) reports
28    as well as conventional TiO2 studies (Boffetta et al., 2004; Fryzek et al., 2003), Liao et al. (2009)  used
29    computer modeling to calculate that exposures to nano-TiO2 (expressed as particle surface area
30    concentrations) were 0.1685 m2 TiO2 per 300 m3 air (working space volume) for packers and 0.387 m2
31    TiO2 per 300 m3 air for surface treatment workers. For nano-TiO2 in the 10- to 50-nm size range, the
32    airborne concentrations (expressed as particle surface area concentrations) were higher in anatase nano-
33    TiO2 than in rutile nano-TiO2 for both packers and surface treatment workers.  The highest airborne
34    concentration was anatase for surface treatment workers, followed in order by  rutile for surface treatment
3 5    workers, anatase for packers, and rutile for packers.
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 1          Liao et al. (2009) also modeled the dose-response relationships from in vitro cytotoxicity studies of
 2    human dermal fibroblasts and inflammatory responses of human lung epithelial cells. They then
 3    compared exposure levels to the dose-response functions and concluded that packers and surface
 4    treatment workers at the studied location were "unlikely to [be at] substantial risk [of] lung inflammatory
 5    response, [but they] have  significant risk [of]  cytotoxicity response at relatively high airborne TiO2
 6    anatase NP [nanoparticle] concentrations at size 10-30 nm" (Liao et al., 2009).
 7          In a presentation at a professional conference, Li et al. (2008b) displayed photographs of a factory
 8    that mixed, but did not manufacture, nano-TiO2.  The photographs appeared to show that nano-TiO2 was
 9    stored in shipping bags piled on pallets. White powder was visible on the facility floor, but its
10    composition is unclear as the factory also handled conventional "pigmentary grade" and "food grade"
11    TiO2 (Ichihara, 2009). Li et al. (2008b) reported that workers had been given masks and shirt-like
12    protective clothing but that the masks were not always worn. The authors also noted that shirt-like
13    protective clothing provided no protection for the forearms and legs of the workers, many of whom wore
14    short-sleeved tops and shorts.  Although this factory may not be representative, it illustrates how
15    inhalation and dermal exposure might occur during the manufacturing or mixing process.
16          As noted in Section 2.3, nano-TiO2 is routinely shipped in paper bags, which could be a source of
17    exposure if they were to be ruptured, punctured, or otherwise compromised during distribution or storage.
18    Nano-TiO2 in dispersion form shipped in pails, drums or totes (Klaessig, 2008) could be subject to
19    accidents resulting from forklift errors, train derailments, and truck accidents, but no empirical data on
20    such incidents specifically related to nano-TiO2 are available.
21          The above information suggests that inhalation and dermal exposure could occur during
22    manufacturing, packaging, shipping, and storage of nano-TiO2.  Without incidence and concentration
23    data, however, the potential for and nature of worker exposure cannot be characterized.


      4.6. Dose

24          Dose is defined as the amount of a substance that actually enters an organism by crossing a
25    biological barrier. Uptake of nano-TiO2 by different routes has  been investigated in various species.
26    Table 4-1 in Section 4.2 summarizes several studies that measured tissue concentrations in fish that had
27    been exposed to nano-TiO2 in water. The exposures included, but were not necessarily limited to, nano-
28    TiO2, appropriately reflecting the multiple substances to which fish can be exposed in the natural
29    environment. For terrestrial organisms, including laboratory animals used for toxicological studies and as
30    models for human health effects, the route of exposure is important in determining the dose that actually
31    enters the body, hence information on uptake  of nano-TiO2 is presented here  according to the route of
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 1    uptake, i.e., inhalation, ingestion, or dermal. Additionally, this section discusses special biological
 2    barriers (blood brain barrier and placenta), and issues related to dose-metrics for nano-TiO2.
      4.6.1. Respiratory (Inhalation and Instillation)
 3          Animal studies have shown that inhaled or instilled nano-TiO2 can translocate into the interstitium
 4    of the lung, lymph nodes (Ma-Hock et al., 2009; Oberdorster et al., 1992; Oberdorster et al.,  1994), blood
 5    (Geiser et al., 2005), and the brain (Wang et al., 2005; Wang et al., 2008b; Wang et al., 2007a).
 6          Particles in the nasal cavity may enter the brain through: (1) the olfactory nerve (Elder et al., 2006;
 7    Oberdorster et al., 2004) [upper particle size limit: 200 nm (Elder et al., 2006)]; (2) the circulating blood
 8    and then crossing the blood-brain barrier (Oberdorster et al., 2004); and (3) the olfactory mucosa and
 9    through the ethmoid bone into cerebrospinal fluid (Ilium, 2000). One of the most visually convincing
10    demonstrations of olfactory nerve transport, as mentioned in (Oberdorster et al., 2004), is a study by De
11    Lorenzo (1970).  De Lorenzo showed sequential transmission electron microscopy (TEM) images  of
12    intranasally instilled gold nanoparticles in the olfactory mucosa, uptake into the olfactory rods, retrograde
13    translocation within the olfactory dendrites, anterograde translocation in the axons of the olfactory nerve,
14    and appearance in the olfactory bulbs.  For more discussion of nanoparticle translocation from the  nasal
15    cavity to the brain, see (Oberdorster et al., 2004).
16          Intranasal instillation of three sizes of nano-TiO2 particles (approximately 20, 70, and  155 nm)
17    resulted in increased titanium concentrations in the olfactory bulb of mice (Wang et al., 2005, 2007a).
18    Also, two forms  of nano-TiO2 particles (80-nm rutile and 155-nm anatase) were found to increase Ti
19    concentrations in the hippocampus, central cortex, and cerebrum, in addition to olfactory bulb, in mice
20    after repeated intranasal instillation (Wang et al., 2008b). The authors noted that the fact that brain tissue
21    Ti concentrations were higher than lung tissue concentrations suggested that the olfactory nerve was the
22    path of transport in this study.
23          For respiratory exposure, the deposition pattern and  concentration of particles in the respiratory
24    tract influence the health effects of these particles.  Particles of various sizes can have different
25    mechanisms of deposition (Gebhart, 1992; Heyderetal., 1985; Oberdorster etal., 2005a). For
26    nanoparticles, diffusive deposition, also known as thermodynamic deposition or diffusion (due to
27    Brownian motion), predominates, whereas for particles larger than  1 (im, aerodynamic deposition
28    predominates. Between 0.1 and 1 (im, the combined effects of aerodynamic and diffusive deposition are
29    important.
30          Oberdorster et al. (2005a) summarized the principles and models of respiratory tract nanoparticle
31    deposition and retention in the lung. Modeling of humans  who are  resting and breathing through the nose
32    indicated that for 1-nm particles, about 90% will be deposited in the nasal, pharyngeal, and laryngeal
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 1     region; about 10% in the tracheobronchial region; and almost none in the alveolar region. These results

 2     contrast with a 5-nm particle, which is deposited roughly equally in the three regions. About 50% of

 3     larger, 20-nm particles are deposited in the alveolar region, with about 15% deposition in each of the

 4     other two regions.

 5           In contrast, a model that incorporates convective flow and axial diffusion predicted that very few

 6     small nanoparticles would deposit in the alveolar area (Asgharian and Price, 2007).  Nanoparticles less

 7     than 10 nm in diameter were predicted to deposit mainly in the tracheobronchial airway, and very few

 8     nanoparticles smaller than 5 nm would reach the alveolar region (Asgharian and Price, 2007). Depending

 9     on particle size, consideration of axial diffusion and dispersion can result in increased predicted

10     deposition in the alveolar region of up to 10%.

11           Inhaled nano-TiO2 persisted in the lung longer than fine TiO2 in rats (Oberdorster et al., 1994).

12     After 12  weeks of inhalation (6 hours/day, 5 days/week) of approximately equivalent mass concentrations

13     of fine TiO2 (22.3 ± 4.2 mg/m3) and nano-TiO2 (23.5 ± 2.9 mg/m3), the total retained lung burdens were

14     6.62 ± 1.22 mg for fine TiO2 and 5.22 ± 0.75 mg for nano-TiO2. The estimated retention half-times were

15     174 days for fine TiO2 and 501 days for nano-TiO2 (Oberdorster et al., 1994).

16           In animal studies of nano-TiO2  disposition (Table 4-3), 13 weeks of inhalation exposure to nano-

17     TiO2 increased TiO2 burden in lymph nodes in rats (2 and  10 mg/m3), mice (10 mg/m3), but not in

18     hamsters (at up to 10 mg/m3) (Bermudez et al., 2004).
       Table 4-3.  Nano-TiCh disposition in animals after inhalation or intratracheal instillation of nano-
                   Ti02.
         Species/strain
   Aerosol
         Study Protocol
           Observations
Reference
       Fischer 344 rats,
       females (6 wks)
       B3C3F1 mice,
       females (6 wks)
       Hamsters, females (6
       wks)
Ti02: 1.29-
1.44umMMAD
(ag = 2.46-3.65),
21-nm primary
particles
Animals exposed via inhalation 6 hours per
day, 5 days per week, for 13 weeks to 0.5, 2,
and 10 mg/m3.
Control animals exposed to filtered air.
Animals sacrificed at 0, 4,13, 26, and
56 days (49 for hamsters) post exposure.
Groups of 25 animals per species and time
point.
Ti02 pulmonary retention half-times for the low-,    Bermudez et
mid-, and high-exposure groups, respectively: 63,  al. (2004)
132, and 365 days in rats; 48, 40, and 319 days in
mice; and 33, 37, and 39 days in hamsters.
Burden of Ti02 in lymph nodes increase with time
post exposure in mid- and high-dosed rats, and in
high-dosed mice, but was unaffected in hamsters
at any time or in any dosage group. In high-
exposure groups of mice, epithelial permeability
remained elevated (~2 x control groups) out to 52
weeks without signs of recovery. Epithelial
permeability was 3 to 4 x control in high exposure
group rats through 4 weeks post exposure, but
approached control by 13 weeks. Epithelial
permeability was unaffected in all groups of
hamsters.
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       Table 4-3. Nano-TiCh disposition in animals after inhalation or intratracheal instillation of nano-
                   TiOz (continued).3
         Species/strain
   Aerosol
          Study Protocol
            Observations
Reference
       Wistar rats, 20 adult
       males, 250±10g
Ti02(22-nm CMD,  Rats exposed 1 hour via endotracheal tube    Distributions of particles among lung
ag = 1.7)        while anesthetized and ventilated at constant  compartments followed the volume distribution of
Spark generated   rate
              Lungs fixed at 1- or 24-hours post exposure
                        0.11 mg/m3
                        7.3x106
                        particles/cm3
                                 compartments and did not differ significantly
                                 between 1- and 24-hours post-exposure. On
                                 average, 79.3±7.6% of particles were on the
                                 luminal side of the airway surfaces, 4.6±2.6% in
                                 epithelial or endothelial cells, 4.8±4.5% in
                                 connective tissues, and 11.3±3.9% within
                                 capillaries. Particles within cells were not
                                 membrane-bound.
                                                                    Geiseretal.
                                                                    (2005)
       WKY/NCrl (Charles
       River) rats, 5 young
       adult males,
       250±10g
Ti02 (22-nm CMD,  Rats exposed 1 hour via endotracheal tube    Of particles in tissues, 72% were aggregates of 2   Kapp et al.
ag=1.7)
Spark generated
while anesthetized and ventilated at constant  or more particles; 93% of aggregates were round  (2004)
rate
Lungs fixed immediately post exposure
or oval; 7% were needle-like. The size distribution
of particles in lung tissues (29 nm CMD, ag = 1.7)
was remarkably similar to the aerosol; the small
discrepancy could have been due to differences in
sizing techniques. A large 350-nm aggregate was
found in a type II pneumocyte, a 37-nm particle in
a capillary close to the endothelial cells, and a
106-nm particle within the surface-lining layer
close to the alveolar epithelium
       aCMD - Count median diameter; MMAD - Mass median aerosol diameter; ag - Geometric standard deviation
       Source: U.S. EPA (2008b).
       4.6.2. Dermal


 1           Because sunscreen is used on the skin, human skin penetration of nano-TiO2 (as particles in

 2     vehicles or in sunscreens) has been discussed in several reports and reviews (NANODERM, 2007;

 3     Nohynek et al., 2007; TGA, 2006). Most dermal exposure studies reviewed used human skin and pig

 4     skin; several were in vivo studies in humans. Compared to other routes of exposure, dermal exposure

 5     may be more directly relevant in assessing potential health effects associated with its use in sunscreens, at

 6     least for unflexed skin from healthy adults.

 7           Because of the relatively noninvasive nature of skin penetration testing, several laboratory studies
 8     have focused on skin absorption in humans, rather than animals.  Human skin regulates the penetration of

 9     contaminants primarily through the stratum corneum layer, which contains keratinized cells and has no

10     blood vessels. The thickness of the layer varies, ranging from hundreds of micrometers to several

11     micrometers in different parts of the body. Published studies indicate the stratum corneum of full-term

12     infants  and babies is comparable to that of adults (Fairley and Rasmussen, 1983); such is not the case with

13     pre-term infants (Kalia et al.,  1998). Skin studies include a range of experimental conditions, including in

14     vivo and ex vivo / in vitro. With few exceptions discussed below (Kertesz et al., 2005; Menzel et al.,

15     2004; Sadrieh et al., 2008), most of these studies (Table 4-4) found clear evidence that nano-TiO2  does not
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 1    penetrate beyond the stratum corneum or hair follicles, and does not penetrate into living cells of healthy
 2    skin (Figure 4-1).
 3          In healthy human skin, topically applied nano-TiO2 penetrates only into the upper layers of the
 4    stratum corneum (Table 4-4).  The pathways of skin penetration can include intracellular penetration,
 5    intercellular penetration, and penetration through hair follicles (Figure 4-1) (Nohynek et al., 2007).
 6    Penetration through sweat glands has not been reported, according to one source (page 29 of
 7    (NANODERM, 2007)). Although increased skin penetration of other nanomaterials has been reported in
 8    flexed skin (Zhang and Monteiro-Riviere, 2008) and in UV-exposed skin (Mortensen et al., 2008), studies
 9    of skin penetration in healthy flexed skin or damaged skin are still underway for nano-TiO2
10          Nano-TiO2 was observed in some hair follicles (Lekki et al., 2007), but did not reach the living
11    follicle cells. The presence of nano-TiO2 in hair follicles is most likely due to mechanical force, such as
12    the movement of the hair during sunscreen application. Nano-TiO2 in hair follicles might contribute to
13    increased Ti levels in the dermis (Sadrieh et al., 2008) because parts of hair follicles are in the dermis.
14    Nanoparticle loss from hair follicles is expected to be slow because the elimination occurs only by its
15    flowing out with sebum or by its being pushed out with sebum. In a study using a hydrogel formulation
16    containing fluorescence-labeled nanoparticles (Resomer RG 50.50 H, poly(lactide-co-glycolide) on
17    human skin (Mittal and Ravi Kumar, 2009), approximately 15% of total nanoparticles detected in hair
18    follicles 30 minutes after application remained in the hair follicle for 10 days, which is at least 10 times
19    longer than particles remain in the stratum corneum (Lademann et al., 2006).
20          In human skin that is diseased, nano-TiO2 might penetrate more deeply. The only available study
21    of nano-TiO2 on skin with dermal lesions was completed on psoriatic skin. Psoriatic skin is a symptom of
22    a chronic, and possibly immune-mediated or genetic, disease called psoriasis. Unlike normal skin cells,
23    which mature and are shed in 28 to 30 days, psoriatic skin cells mature in 3 to 4 days, accumulate on the
24    skin surface (instead of shedding, because new skin develops faster than dead skin sheds), and develop
25    into patches of dead skin (National Psoriasis Foundation, 2006; Pinheiro et al., 2007).  Psoriatic skin has a
26    looser corneocyte organization than healthy skin due to the loss of stratum corneum cohesion (Pinheiro et
27    al., 2007).  In the Pinheiro et al. (2007) study, nano-TiO2 in a sunscreen formulation penetrated into
28    deeper areas of the stratum corneum in psoriatic  skin than in healthy skin, but not into living cells in
29    either psoriatic or healthy skin (Table 4-4).
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          Stratum corneum
            Stratum lucidum
       Stratum granulosum
          Stratum spinosum
             Stratum basale
         Sebaceous gland
                 Hair follicle
     Apocrine sweat gland

      Matrix of hair follicle
             Blood vessels
                                      Epithelium
                                                                                               Dermis
                                                                                                 Sweat Gland
                                      Hypodermis
                                                                                             — Stratum corneum
                                                                                            =i- Stratum lucidum
                                                                                            j- Stratum granulosum
                                                                                             - Stratum spinosum
                                                           Adapted from: Monteiro-Reviere (1991 ; 2004) and Nohynek et al. (2007).

     Figure 4-1.      Possible pathways of nano-TiCh skin penetration.
       TOP GRAPHIC - Nanoparticles may penetrate into skin by passing through the (1) intercellular space between cells, (2) skin cells, (3) opening of
       hair follicles, or (4) opening of sweat glands. Nano-Ti02 has been seen in the stratum corneum and inside hair follicles, but not in sweat glands.
       BOTTOM GRAPHIC - Skin surface (from stratum corneum to stratum granulosum) at a high magnification showing simplified paths of
       nanoparticles passing through (1) intercellular space and (2) skin cells.
     Nanoparticles are not drawn to scale in either graphic.
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Table 4-4.  Overview of TiOz skin absorption/penetration studies.3
                            Test Material
                                                        Skin Modelb
                                                   (Sampling Technique)
                                                         Results
                                                                   Reference
Sunscreen Formulations Containing Nano-TiCh
Nano-Ti02 in a sunscreen
formulation
Primary particle 17nm (Kemira, 2000), rutile,
AbOs/stearic acid coated, aggregates 150 to 170
nm (UV-Titan M 160) in an oil-in-water emulsion,
provided by L'Oreal (Clichy, France)
Human forearm, repeated
application for 4 days (tape
stripping, biopsy)
Most particles on and in the upper layers of stratum corneum.  In
the lower half of the horny layer, only in the openings of hair
follicles and sebaceous glands.  In deeper tissue, exclusively in the
follicle channels.
No penetration into living skin.
Lademannetal. (1999)
Sunscreen that contains
nano-Ti02
Not specified
Human skin (healthy and
psoriatic), in vivo, 2 hr (biopsy)
Deeper nano-Ti02 penetration in psoriatic skin than in healthy skin.
No penetration beyond stratum corneum in both psoriatic and
healthy skin.
Pinheiroetal. (2007)
Nano-Ti02 in a sunscreen
formulation
20-nm nano-Ti02, coated with silicone
Human skin, in vitro, and human
skin, in vivo (skin stripping)
Penetration limited to upper layers of stratum corneum.
Nanoparticles in skin furrows or follicular opening could be
mistaken to be in the epidermal compartment.
Mavonetal. (2007)
Sunscreen that contains
nano-Ti02
A commercially available sunscreen, hydrophobic
emulsion containing nano-Ti02 (Anthelios XL SPF
60, La Roche Posay, France)
Human foreskin grafts
transplanted onto SCID mice;
Ti02 emulsion on the graft in
occlusion for 1, 24, or 48 hr
Ti02 in the corneocyte layers of stratum corneum.
In two cases, penetration through the stratum corneum, to the
stratum granulosum was observed.
Kerteszetal. (2005)
Sunscreen that contains
nano-Ti02
A commercially available sunscreen, hydrophobic
emulsion containing nano-Ti02 (Anthelios XL SPF
60, La Roche Posay, France)
Human foreskin grafts
transplanted onto SCID mice;
Ti02 emulsion on the graft at 2
mg/cm2 in occlusion for 24 hours
Ti02 in stratum corneum, not in deeper layers of the skin.
Various Ti02 in sunscreen
formulations
Sunscreen base formulation containing no Ti02 or
5% of one of three types TiCt
  Micro-sized Ti02
  Nano-Ti02, uncoated
  Nano-Ti02, coated with aluminum hydroxide and
  dimethicone/methicone copolymer
Female Yucatan minipigs (in
vivo), 2-mg emulsion/cm2 skin, 5
days per week for 6 weeks
(necropsy)
Increased Ti levels in epidermis in all TiCVtreated groups.
Increased Ti levels in dermis in some TiCVtreated groups (not
specified).
No increases in Ti levels in lymph nodes or liver of any treated
animals.
Kiss etal. (2008)
Nano-Ti02in sunscreen
formulation /Sunscreen
that contains nano-Ti02
50 to 100 nm, mixture of anatase and rutile, no
coating information
Human abdominal skin, in vitro Penetration limited to upper layers of stratum corneum.
Dussert and Gooris
(1997)
Sadriehetal. (2008)
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Table 4-4.  Overview of TiOz skin absorption/penetration studies (continued).3
                            Test Material
                                                       Skin Modelb
                                                  (Sampling Technique)
                                                        Results
                                                                  Reference
Photostable nano-Ti02 in
various formulations
Photostable nano-TiC^. needle-like shape, 45-150
nm x 17-35 nm, coated with alumina and silica
(Loden et al., 2006), in the following formulations:
(1) Eucenn® Micropigment Creme 15: commercial
sunscreen, 5% Ti02 concentration (Beiersdorf
company)
(2) a liposome dispersion: 18% Ti02, containing
Phospholipon 90 G and Tioveil AQ-N (Tioxide
Specialties Ltd., Billingham, UK)
(3) formula SG110:  4.5% Ti02, containing Tioveil
AQ-N
(4) pure predispersion Tioveil AQ-N:  40% Ti02
Pig skin, in vitro
Particles on/in the stratum corneum; minimal penetration into
stratum granulosum.
No penetration into living skin.
Menzeletal. (2004)
Photostable nano-Ti02 in
sunscreen formulations
(1) T-Lite SF-S: rutile, coated with Si02 and
methicone
(2) T-Lite SF: rutile, coated with methicone
 Both primary particles are needle-like: 30-60 nm
 x 10 nm. Aggregates and agglomerates in water
 phase, mostly up to 200 nm
 Both are oil/water emulsions containing 10% Ti02
Pig skin, in vitro, up to 24 hours
(tape stripping)
No penetration beyond stratum corneum.
Receptor solution recoveries of 0.8-1.4% of applied dose.
Gamer etal. (2006)
Other Nano-Ti02 Formulations
UV-TitanM160®
                         "Microcrystalline," coated
                                               Human, in vivo
                               Most Ti02 in the superficial part of the stratum corneum.  Some
                               Ti02 in follicles (in the deeper layers of the stratum corneum).
                                                            Ref62,70inSCCNFP
                                                            (2000)
Various nano-Ti02inoil-
in-water emulsions
Emulsions contained 4% nano-Ti02, only differed in
nano-Ti02 types:
(1) 20-nm cubic primary particle, coated with
trimethyl octylsilane, hydrophobic surface (T805,
Degussa)
(2) 10-15 nm primary particle, aggregated into
~100-nm needles, coated with AbOs and Si02,
amphiphilic surface (Eusolex T-2000, Merck)
(3) 100-nm needles, coated with alumina and silica,
hydrophilic surface (Tioveil AQ-10P, in dispersion,
Solaveil)
Human forearm, in vivo, 6 hours
(biopsy)
Penetration of particles into the upper layers of stratum corneum.
No penetration into living skin.
Pfluckeretal. (2001)and
Schulzetal. (2002)
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Table 4-4. Overview of TiOz skin absorption/penetration studies (continued).3

Anatase (Tioveil AQ-N)
Nano-Ti02
Various Ti02 and nano-
Ti02
Degussa T805
Eusolex TA and Eusolex
TC
Eusolex TA and Eusolex
TC
HombifineS35
Tioveil AQG, Tioveil TG,
and Tioveil OP
Test Material
"Microcrystalline," coated with alumina and silica
10-100 nm, coated with Si02-,AI203-, AI203l/Si02
14-nm to 200-um, anatase and rutile, coated and
uncoated materials
21 nm, coated with Si02
"Microcrystalline," coated
"Microcrystalline," coated
"Microcrystalline," coated
"Microcrystalline" (though SCCNFP not 100%
certain)
Skin Model b
(Sampling Technique)
Human, repeated application
(tape stripping)
Human, in vivo (biopsy)
Pig and human skin, in vivo and
in vitro (skin stripping or biopsy)
Human, in vitro
Human, in vitro
Human
Human, in vitro, and mouse, in
vitro
Human
Results
No penetration beyond the stratum corneum. Some Ti02 at the
opening of follicles.
Particles on or in the outmost surface of the stratum corneum.
No penetration into living skin.
No penetration beyond the stratum corneum in any study.
No penetration beyond the stratum corneum.
No penetration beyond the stratum corneum.
No penetration beyond the stratum corneum.
No penetration beyond the stratum corneum.
No penetration beyond the stratum corneum.
Reference
Ref. 63 in SCCNFP
(2000)
Schulzetal. (2002)
SCCNFP (2000)
Ref. 24 in SCCNFP
(2000)
Ref. 25 in SCCNFP
(2000)
Ref. 26 in SCCNFP
(2000)
Ref. 27 in SCCNFP
(2000)
Ref. 29 in SCCNFP
(2000)
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Table 4-4.  Overview of TiOz skin absorption/penetration studies (continued).3
                            Test Material
                                                       Skin Modelb
                                                  (Sampling Technique)
                                                         Results
                                                                  Reference
Degussa T805
Ti02
21-nm, coated with Si02
Mixed particle sizes, mostly less than 10 urn in
aqueous solution (range from <2 urn to >20 urn), no
coating information, 20% Ti02 in water, castor oil, or
polyethylene glycol
Human, in vitro
Rabbit skin, in vivo, 4 hours for
1 day or 2 hours daily for 3 day
No penetration beyond the stratum corneum.
Penetration of particles into stratum corneum and outer hair
follicles.
No penetration into living skin.
Ref. 112inSCCNFP
(2000)
Lansdown and Taylor
(1997)
                                                                                                        polyethylene glycol.
Nano-Ti02 in various gels
For ion microscopy study: 20-nm x 100-nm primary
particles, coated (photostable UV-filter) (Eusolex® T-
2000, Merck).  Four formulations: hydrophobic
basis gel, isopropyl myristate gel, microemulsion
gel, and polyacrylate gel, each containing 5%-
weight nano-Ti02 particles
For autoradiography study: proton-irradiated 20-nm
Ti02, rutile (R-HD2, Huntsman), coated with
alumina (Huntsman, 2008)
Porcine and human skins, for
30 minutes to 48 hours (biopsy)
After wash with water, nano-Ti02 remains on skin, with most in
stratum corneum and some in hair follicles.
Nano-Ti02 observed seen in hair follicles as deep as 400 pm, but
not in living cells surrounding the follicles.
Lekkietal. (2007)
Ti02/Nano-Ti02 Particles of Unknown Size
Sunscreen that contains
Ti02
Not specified
Human (tape stripping)
Particles on or in the outmost layers of the stratum corneum.  No      Gottbath and Mueller-
penetration into living skin.                                       Goymann (2004)
Ti02
Not specified
Mouse, pig, and human skin, in
vitro
Ti02 detected in the intercellular spaced between corneocytes of
the outermost layers of the stratum corneum. No penetration into
living skin.
Gontieretal. (2004)
Sunscreen that contains
Ti02
Sunscreen containing 8% microfine Ti02 (size,
crystal form, and coating were not specified)
Human skin (13 patients, 59-82
years old), in vivo, applied Ti02
sunscreen daily for 9-31 days
until 2 days prior to surgical
removal of the skin (tape
stripping)
Ti concentration in the dermis of patients exposed to sunscreen
overlaps with concentration in cadavers (controls).  If the highest Ti
concentration in cadavers is excluded, sunscreen increased skin Ti
concentration.
No correlation between the duration of sunscreen application and
Ti concentration.
Tan etal. (1996)
a SCID - Severe combined immunodeficiency; SCCNFP - Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers
b Topical application unless specified.
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 1          Mortensen et al. (2008), working with quantum dots rather than TiO2, reported greater skin
 2    penetration following UV exposure and suggested that even mildly sunburned skin might be more
 3    susceptible to penetration by nanoparticles of similar size and chemistry to the quantum dots used in their
 4    study.
 5          Using "microfine" TiO2, Tan et al. (1996) compared uptake in skin samples from 13 elderly persons
 6    (age 59-82 years) with samples from 6 control cadavers (used to determine background exposure).  The
 7    authors reported some dermal uptake, although they suggested caution when interpreting their results,
 8    citing the advanced age of their participants, the fact that skin samples were taken from different
 9    locations, and the fact that TiO2 concentrations were close to analytical detection limits. Kertesz et al.
10    (2005) reported penetration of nano-TiO2 into the stratum granulosum of grafted human foreskin in two
11    samples (of an unknown total number).
12          Penetration of nano-TiO2 into dermis of minipigs was suggested in a meeting abstract, but the
13    abstract contained insufficient information to determine whether Ti was detected inside the hair follicles
14    or in the living cells of the dermis (Sadrieh et al., 2008). Several other studies that evaluated absorption
15    using pig skin suggest little or no absorption beyond the stratum corneum. In a study using nano-TiO2 in
16    four formulations on pig skin (Menzel et al., 2004), the authors  stated that nano-TiO2 penetrated through
17    the stratum corneum into the underlying stratum granulosum (but not into stratum spinosum) via
18    intercellular space. The presence of Ti in the dermis, however, was deemed to be an artifact of the
19    preparation process. Other studies using pig skin did not find nano-TiO2 penetration beyond the stratum
20    corneum (Gamer et al., 2006; Lekki et al.,  2007; Pflucker et al., 2001).
21          Some nanomaterials have been shown to penetrate deeper in damaged skin than in intact skin
22    [quantum dots in human skin (Mortensen et al., 2008); nano-silver in murine skin (Larese et al., 2009)],
23    but no experimental data on nano-TiO2 dermal penetration in damaged skin were found. Preliminary (not
24    yet peer reviewed) data showed that two types of coated nano-TiO2 topically applied on either
25    dermabraded or intact skin of SKH-1 hairless mice did not increase Ti concentrations in blood, lymph
26    nodes, liver, spleen, or kidney (Gopee et al., 2009).  The depth of nano-TiO2 penetration in either
27    damaged or intact skin was not reported. Hairless mice data, however, do not exclude the possibility that
28    nano-TiO2 might penetrate deeper into damaged human skin than intact human skin because relative
29    penetration of chemicals between hairless mice and humans varies and could be chemical specific
30    (Benavides et al., 2009; Simon and Maibach, 1998).

      4.6.3. Ingestion

31          Currently only three toxicological studies of nano-TiO2 through oral exposure have been reported
32    (see Section 5.3.1.2.2), and of these, only one (Wang et al., 2007a) reported tissue concentrations of nano-
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 1     TiO2. In the Wang et al. (2007a) study, male and female mice received a single oral gavage of 5 g/kg TiO2

 2     as 25-nm rutile spindles, 80-nm rutile spindles, or 155-nm anatase octahedrons (10 male and 10 female

 3     mice for each type of TiO2, and negative controls) (Table 4-5). The organs with elevated TiO2

 4     concentrations (measured only in female mice) were liver, spleen, kidney, lung, and brain. Although the

 5     liver is expected to receive most of the TiO2 absorbed from the gastrointestinal tract through the portal

 6     vein, elevated TiO2 levels in the liver were observed only in the 80-nm group.  The reason for this size-
 7     specific elevation in hepatic TiO2 concentration is unknown.



       4.6.4.  Blood  Brain Barrier and  Placental  Transfer


 8           The potential of nanoparticles in general to cross the blood brain barrier (BBB) has been

 9     investigated and developed primarily in relation to drug delivery systems (Beduneau et al., 2007; Emerich

10     and Thanos, 2007). In addition to size (Sonavane et al., 2008), the surface properties of nanoparticles

11     influence the potential for a nanomaterial to penetrate the BBB (Singh and Lillard, 2009).  Nanoparticles

12     developed for drug delivery often have ligands conjugated on the surface or other  surface modifications to

13     facilitate cellular uptake (Beduneau et al., 2007).
       Table 4-5. Animal studies that measured Ti concentrations in brain after nano-TiCh exposures
                  through injection or oral gavage.3
             Nano-TiCh
           Study design
        Findings in the brain
 Reference
       Nano-Ti02, 25 nm and 80 nm,
       rutile, uncoated (from
       Hangzhou Dayang
       Nanotechnology Co. Ltd.,
Single oral gavage at 5 g/kg to male and female
CD-1(ICR)mice
Ti content was measured 2 weeks after gavage
by ICP-MS with a detection limit of 0.074 ng/mL
       FineTi02, 155±33 nm Ti02,
       anatase, uncoated, > 10 wt% at
       <100 nm (from Zhonglina
       Chemical Medicine Co.,
Ti concentrations in brain were increased in all
three Ti02 treatment groups compared to
negative controls. The increase was smaller in
the 25-nm group than the 155-nm group, while
the 80-nm group had the same increase as the
155-nm group.
Vacuoles in the neuron of hippocampus,
suggesting fatty degeneration, observed in the
80-nm (but not typical) and 155-nm (frequently)
groups, but not in the 25-nm group.
       (Chen, 2008)
Wang etal.
(2007a)
       Nano-Ti02, 20-30 nm, 17%
       anatase, 30% rutile, uncoated,
       BET surface area 48.6 m2/g
Single i.v. injection at 5 mg/kg BW through the
tail vein of male Wistar rats
Ti02 concentrations in the brain were measured
on days 1,14, and 28 by ICP-AES with a
Thermo Jarrell Ash "IRIS 1" spectrometer with a
detection limit of 0.5 pg/organ
Ti02 was not detected in the brain at any tested    Fabian et al.
time points.                            (2008)
       Nano-Ti02,15 nm, rutile,
       coated with silica (27.5 wt%)
Single i.v. injection at approximately 60 mg/kg
BW through the tail vein of male ddY mice
Ti concentrations in brain were measured at 5
minutes, 72 hours, and 1 month after injection by
ICP-MS with an unspecified detection limit
No increase of Ti in the brain of treated mice      Sugibayashi
was observed compared to negative controls at    et al. (2008)
any tested time points.
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       Table 4-5. Animal studies that measured Ti concentrations in brain after nano-TiCh exposures
                  through injection or oral gavage (continued).3
             Nano-TiCh
           Study design
        Findings in the brain
 Reference
       Nano-Ti02, 5 nm, anatase
       Conventional Ti02
       Both types ofTi02were made
       from controlled hydrolysis of
       titanium tetranutoxide.
Multiple i.p. injection to female CD-1 (ICR) mice
once per day for 14 days with nano-Ti02 at 5,
10, 50,100, and 150 mg/kg BW or conventional
Ti02at150mg/kgBW
Ti concentration was measured 14 days after the
treatment began by ICP-MS with a detection
limit of 0.076 ng/mL
Ti concentrations in the brain increased with
increasing nano-Ti02 doses. All Ti02 treatments
increased Ti concentration in the brain, as
compared to negative controls. At 150 mg/kg,
brain Ti concentration was higher in the nano-
Ti02 group than in the conventional Ti02 group.
Liu etal.
(2009)
       Nano-Ti02, 25-70 nm, anatase,
       surface area 20-25 m2/g, purity
       99.9% (from Sigma-Alderich)
Subcutaneous (s.c.) injections of 100 pL of
1 mg/mL nano-Ti02 (i.e., 0.1 mg nano-TiCy
each time per pregnant Slc:ICP mice once per
day at 3, 7, 10 and 14 days post-mating.
Presence of nano-Ti02 in the brain was
assessed in the male offspring at age of 4 days
and6weeksbyFE-SEM/EDS
Nano-Ti02 particles were seen in the brain
(olfactory bulb and the cerebral cortex - frontal
and temporal lobes) of the 6-week-old mice from
nano-Ti02-exposed dams. (Results from 4-day-
old mice were not reported.)
Markers of apoptosis (activation of caspase-3
and crescent-shaped cells), occlusion of small
vessels, and perivascular edema observed in
the brain of 6-week-old mice from nano-Ti02-
exposeddams.
Takedaetal.
(2009)
       a BET - Brunauer, Emmett, Teller method of calculating surface area
       BW-Body weight
       FE-SEM/EDS - Field emission-type scanning electron microscopy/energy
         dispersive X-ray spectrometry
       ICP-AES - Inductively coupled plasma atomic emission spectrometry
                              ICP-MS - Inductively coupled plasma-mass spectrometry
                              i.p. - Intraperitoneal
                              i.v. - Intravenous
                              s.c.-Subcutaneous
 1           Increased Ti concentrations in the brain were observed in mice 2 weeks after they were exposed to

 2     fine and nano-TiO2 through a single oral gavage (Wang et al., 2007a), and in mice at the end of exposure

 3     to nano-TiO2 through once-daily intravenous injections for  14 days (Liu et al., 2009) (Table 4-5).  No

 4     increase in Ti concentration in the brain was observed in rats or mice exposed to nano-TiO2 through a

 5     single intravenous injection (Fabian et al., 2008; Sugibayashi et al., 2008).  Due to the variations in tested

 6     nano-TiO2, treatment regimen, and other experimental design elements, no specific characteristic of nano-

 7     TiO2 or its administration has been identified as determining factors for BBB penetration.

 8           A recent study showed TiO2 particles and pathological changes in the brain of 6-week-old mice

 9     from nano-TiO2 exposed dams (Takeda et al., 2009) (Table  4-5), suggesting that nano-TiO2 might be

10     passed through undeveloped  or developing BBB in embryos or young mice. Because the dams were

11     exposed to nano-TiO2 during pregnancy and the offspring were tested at 4 days and  6 weeks of age, the

12     nano-TiO2 exposure to the offspring could have been in utero (i.e., nano-TiO2 could penetrate the

13     placental barrier) or through milk, which was not tested in this study. In addition to the brain, nano-TiO2

14     particles and pathological changes were also observed in the reproductive system of male offspring of

15     nano-TiO2-exposed dams (female offspring were not studied) (Takeda et al., 2009).  Although no data on

16     humans for nano-TiO2 and placental barrier were located, an ex vivo study using perfused human

17     placentas showed that nano-gold (PEGylated gold nanoparticles at 15 and 30 nm) did not cross the

18     placenta into the fetal circulation at the tested condition (Myllynen et al., 2008). Nano-gold might behave
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 1    differently from nano-TiO2, given that uncoated nano-gold does not penetrate either the BBB or placental
 2    barrier in mice (Sadauskas et al., 2007), whereas nano-TiO2 does pass to BBB in mice (Liu et al., 2009;
 3    Wangetal., 2007a).

      4.6.5. Dose-Metrics

 4          Quantitative risk assessment requires dose-response relationships.  Selecting a measure of dose that
 5    is appropriate for nanoparticle toxicity has drawn attention from both researchers and risk assessors. No
 6    one metric is recommended in this case study, but supporting evidence for various selections of a dose
 7    metric is noted. The criterion for selecting a "good" dose metric is often based on generating a consistent
 8    dose-response relationship. However, an appropriate dose metric need not constitute measurement of
 9    only one physicochemical property (such as surface area, mass, or number of particles). Although dose
10    metrics based on one property, such as mass concentration, have been used successfully in toxicology, a
11    combination of measurements of two or more physicochemical properties also might be appropriate for
12    use in assessing nanomaterial toxicity.
13          Total particle surface area, which is closely related to primary particle size, has been suggested as a
14    suitable dose metric for inhalation  and instillation studies (Faux et al., 2003; Liao et al., 2008;
15    Oberdorster et al., 2005). Although two distinctive dose-response curves for fine TiO2  and nano-TiO2 can
16    be drawn based on mass concentration, certain observed respiratory effects of fine TiO2 and nano-TiO2
17    have been shown to fit well with a single  linear dose-response curve based on primary particle surface
18    area, even where both types of particles agglomerated to about 0.7  (im in diameter (Oberdorster et al.,
19    1994). Hext et al. (2005) found that, compared to gravimetric lung burden (particle mass per lung mass),
20    administered primary particle surface area correlated better with lung burdens, clearance half-lives, and
21    certain biological responses in rats, mice, and hamsters.  However, the evidence on this issue is somewhat
22    mixed. For instance, biological responses after exposure to similarly-sized agglomerates of fine TiO2 and
23    nano-TiO2 were similar in severity according to Warheit et al. (2007c; 2006); by contrast, Sager and
24    Castranova (2009) found that well-dispersed nano-TiO2 yielded greater effects than well-dispersed fine
25    TiO2
26          As mentioned previously, any one or more of various characteristics, including particle number,
27    size (including agglomerations or aggregations), shape, crystalline form, mass, surface  area, and surface
28    modifications, could play a role in  nano-TiO2 toxicity. Including one or more of these factors in the dose
29    metric could be a better choice than surface area alone.  For instance, based on administered primary
30    particle surface area, the data used in the Hext et al. study (2005) - the increases in the  numbers of
31    pulmonary polymorphonuclear neutrophil (PMN) due to exposure to anatase fine and nano-TiO2
32    (Oberdorster et al., 1994) and rutile fine TiO2 (Tran et al., 1999) - would better fit two dose-response
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 1    curves (one each for anatase TiO2 and rutile TiO2), instead of one dose-response curve.  Similarly, a recent
 2    study of pulmonary effects of intra-tracheal instilled rutile fine TiO2 and 80% anatase/20% rutile nano-
 3    TiO2 (Sager et al., 2008) showed that when dose was normalized to surface area of the particles
 4    administered, the dose-response curves for inflammogenic responses were not statistically different
 5    between fine and nano-TiO2, but the anatase-rutile nano-TiO2 always yielded greater (1.3- to 2-fold)
 6    responses than the rutile fine TiO2.
 7          Due to limited toxicological data from oral  or dermal exposure to nano-TiO2, the choice of dose
 8    metric for these exposure routes has not been widely discussed.  For in vitro studies, nanoparticle
 9    concentration (mass or surface area) is often used  to express dose. In vitro cytotoxicity, however, has
10    been reported to be affected by both the concentration and the total mass (or total number or total surface
11    area, since these three  are closely related) of nanoparticles (Lison et al., 2008). In the Lison et al. study
12    (2008), when cells were cultured in various volumes of a medium containing the same amount of nano-
13    silica (same mass/number/surface area), higher toxicity occurred in a lower volume of medium, that is, in
14    higher nano-silica concentrations.  When the medium contained the same concentrations of nano-silica,
15    higher toxicity occurred in cells cultured with a higher volume of medium than lower volume of medium.
16
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         Questions about  Exposure-Dose  Characterization


    4-1.  Which sources, pathways, and routes pose the greatest exposure potential to nano-Ti02 for biota? .. .for humans?

    4-2.  What is the potential for biota and human (both occupational and general population) exposure to secondary
         contaminants (e.g., waste or transformation products) associated with the entire life cycle of water treatment or
         sunscreen applications of nano-Ti02?

    4-3.  Do particular species of biota and populations of humans have greater exposure potential (e.g., high-end exposures
         due to unusual conditions or atypical consumption)?  In particular, do children get a higher exposure and/or dose?

    4-4.  What is the total population that could be exposed to nano-Ti02 via drinking water? ...via topical sunscreens?

    4-5.  Approximately how many workers are involved in nano-Ti02 production, distribution, and use?

    4-6.  What concentrations, routes, frequencies, and durations characterize worker exposures to nano-Ti02 across the life
         cycle and within certain stages (e.g., manufacturing)?

    4-7.  What management practices exist to control occupational exposures to nano-Ti02?

    4-8.  What personal protective equipment do workers use at the various life cycle stages of nano-Ti02 applications? How
         effective is such equipment in controlling exposures by all routes?

    4-9.  Are occupational monitoring methods available or in place for all relevant stages of the life cycle for nano-Ti02
         applications?

    -10.  Are available methods adequate to characterize nano-Ti02 exposure via air, water, and food? What properties of
         nano-Ti02 should be included in such exposure characterizations?

    -11.  Given the potential for greater uptake of certain substances in the presence of nano-Ti02, should  monitoring and
         exposure studies include a suite of substances that might interact with  nano-Ti02?

   4-12.  What happens when nano-Ti02 is trapped in the stratum corneum and the dead skin flakes off? Is there a potential for
         dead-skin nano-Ti02 to settle around households, or be inhaled?  How much might accumulate after a day (or a few
         days) in the sun (and numerous reapplications)?

   4-13.  Since nano-Ti02 may increase the uptake of other pollutants, such as arsenic, would nano-Ti02 be a greater concern
         for exposure and ecological effects in areas with high concentrations of certain pollutants than in other areas?  If so,
         how do we predict or identify such "hot spots?"

   4-14.  Which, if any, exposure models have been evaluated for applicability to nano-Ti02?

   4-15.  Which physiologically-based pharmacokinetic models are optimal  for understanding absorption, distribution, and
         elimination of nano-Ti02 in humans?

                                                                                 Questions continued on next page.
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        Questions about Exposure-Dose Characterization
  4-16.  Are exposure-dose models available (and adequate) to quantitatively extrapolate the exposure used in animal
        toxicology studies (by inhalation, instillation, oral, dermal, and in vitro) to the human exposure that would result in an
        equivalent dose to the target of interest?

  4-17.  What is the potential for nano-Ti02 to transfer to or accumulate in the food web and cause adverse effects on
        ecological receptors?

  4-18.  Nano-Ti02 has been shown to attach to the surfaces of algae and fish as well as bioaccumulate in fish.  Does nano-
        Ti02 biomagnify?
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           Chapter 5.  Characterization  of  Effects

 1         The preceding chapters have laid a foundation for the present chapter by providing an exposure
 2    context for characterizing the effects of nanoscale titanium dioxide (nano-TiO2) used for water treatment
 3    and in topical sunscreens. This chapter provides information on the factors that influence nano-TiO2
 4    ecological and health effects (Section 5.1), the ecological effects of nano-TiO2 (Section 5.2), and the
 5    toxicological and human health effects of nano-TiO2 (Section 5.3). Whether there are specific by-
 6    products (e.g., waste and transformation products) or interactions with other substances that should or can
 7    be evaluated has not yet been determined. For this reason, the focus of this chapter is on nano-TiO2.
 8         Although literature exists on the effects of conventional TiO2 on humans and laboratory animals
 9    [for a review, see NIOSH (2005)], comparatively less information is available on the effects of nano-TiO2.
10    Consistent with studies of other nanomaterials (Ostrowski et al, 2009), most nano-TiO2 studies have
11    investigated the ecological or health effects of nano-TiO2 itself, and relatively few have investigated the
12    ecological or health effects of end-use products containing nano-TiO2 or their life-cycle by-products.
13         The physicochemical characteristics of nano-TiO2 could be important to the biological effects of
14    these materials (Section 5.1), yet those characteristics frequently are not evaluated or reported as part of
15    studies of such effects. This observation should serve as a caveat in examining and interpreting the
16    results described throughout this chapter.
17         The following sections are not meant to be an exhaustive review of the ecological and human
18    health effects literature for nano-TiO2. Instead, this chapter is intended to highlight recent work on the
19    effects of nano-TiO2 and to identify current knowledge status and gaps in information needed for
20    assessing potential risks of nano-TiO2 in water treatment and sunscreen.
      5.1. Factors that Influence Ecological and Health Effects  of
        Nano-TiOz
21         The large number of variables associated with nano-TiO2 material itself and its ecological and
22    health effects makes it extremely difficult to identify the primary characteristic(s) of nano-TiO2
23    contributing to an effect or to compare the importance of different characteristics to such effects. A
24    common statement from early studies is the announcement of size effects (or the lack of size effects) from
25    nano-TiO2 of different crystalline forms or anatase/rutile ratios. That size alone does not account for the
26    effects of nano-TiO2, however, is now generally accepted; other factors, such as shape, surface chemistry,

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 1    photoreactivity, and other characteristics, could also play a role in these effects (Gonzalez et al., 2008;
 2    Hassellov et al., 2008; Powers et al., 2006). With the advance of nanoparticle synthesis, the influence of
 3    different physicochemical characteristics of nano-TiO2 has been investigated using well-characterized
 4    nano-TiO2 and better control of variables in recent studies (Jiang et al., 2008).
 5          Three categories of factors (nano-TiO2 physicochemical characteristics, experimental conditions,
 6    and environmental conditions) that could influence the ecological and toxicological or health effects of
 7    nano-TiO2 are discussed here in Section 5.1. These are not the only factors of potential importance.  As
 8    noted previously, exposure route can play a major role  in the effects of nano-TiO2, and the importance of
 9    this is reflected in the fact that much of the information in this chapter is organized around environmental
10    media and routes of exposure. Host effects, particularly species differences, can also play an important
11    role in the effects of nano-TiO2. For example, skin penetration is greatest in rabbits, followed by rats,
12    pigs, monkeys, and humans (Nohynek et al., 2007). However, little information is available on these
13    species differences or on differences in susceptibility of different cell types to nano-TiO2 effects (Kiss et
14    al., 2008). The phenomenon of pulmonary particle clearance "overload" and subsequent effects in rats
15    and mice are much more understood and are discussed in Section 5.3.1.2.3.  In the following sections, the
16    order in which factors are presented does not imply relative importance.  This section focuses on factors
17    that have been shown to be important for nano-TiO2, but findings related to other types of nanomaterials
18    are noted where relevant.
      5.1.1. Nano-TiCh Physicochemical Characteristics
19          Size, crystal structure, and surface chemistry (such as coating) are among the factors that influence
20    nano-TiO2 effects.  Other physicochemical properties, such as shape (Warheit et al., 2006; Yamamoto et
21    al., 2004), manufacturing process, doping, and purity (or impurities) could also play a role in nano-TiO2
22    toxicity, but such information is usually not reported in ecological and toxicological studies.  Contributing
23    to this lack of reported characteristics are limitations in the availability of analytic methods for
24    characterizing such nanomaterials. Databases describing detailed nanoparticle properties and health
25    effects are being developed (Miller et al., 2007a).
26          The need for characterization of nanomaterials used in toxicity studies has been noted in reports
27    and journal articles, with possible attributes for minimal characterization including chemical composition,
28    size and size distribution (for primary particles and agglomerates), shape, specific surface area, and
29    number of particles per unit mass (Department for Environment Food and Rural Affairs, 2007; Powers et
30    al., 2006; Powers et al., 2007; Warheit et al., 2007a). For more information on nanomaterial
31    physiochemical characteristics that could affect ecological and toxicological effects, readers are referred

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 1    to reports listing recommended information to be included in nanomaterial studies (OECD, 2008;
 2    Attachment 5 of Taylor 2008; Warheit et al, 2007c).

      5.1.1.1. Size
 3          Size is a main determining factor for the distribution of (inhaled or instilled) nano-TiO2 in and
 4    outside of the respiratory tract (Oberdorster et al., 2004).  For particles with a diameter less than 100 nm,
 5    the smaller the particles are, the more total particle deposition in the respiratory tract and deposition in
 6    nasopharyngolaryngeal regions (Oberdorster, 2000).  Smaller sizes, however, do not always result in more
 7    deposition in other regions of the respiratory tract.  For example, the highest percentages of alveolar
 8    deposition have been observed in nanoparticles of about 20 nm in size, and the highest percentages of
 9    tracheaobronchial deposition were observed in nanoparticles 1-10 nm in size (Oberdorster, 2000).
10    Furthermore, particles less than 200 nm in size can be transported from olfactory mucosa to the olfactory
11    bulb of the brain via the olfactory nerve (Elder et al., 2006).  Exposures to nano-TiO2 (with mean
12    diameters of 21.05 ± 5.08 nm, 71.43 ± 23.53 nm, and 154.98 ± 32.98  nm) through intranasal instillation
13    increased titanium concentrations in the olfactory bulb in mice (Wang et  al., 2005; Wang et al., 2007a),
14    and two types of nano-TiO2 particles (80-nm rutile and 155-nm anatase) were found to increase Ti
15    concentrations in hippocampus,  central cortex, and cerebrum, in addition to olfactory bulb, in mice after
16    repeated intranasal instillation (Wang et al., 2008b).
17          Jiang et al. (2008) investigated the size effects of nano-TiO2 on reactive oxygen species (ROS)
18    generation per unit of particle surface area. Using nine different sizes (4-195 nm) of anatase nano-TiO2,
19    the investigators found that the highest levels of ROS generation per unit surface area were generated by
20    30-nm and larger particles.  For nano-TiO2 less than 30 nm, the ROS generation per surface area
21    decreased with decreasing particle diameter down to  10 nm, below which it was constant (Jiang et al.,
22    2008).

      5.1.1.2. Crystallinity
23          TiO2 crystalline forms also influence TiO2 and nano-TiO2 photoreactivity, reactive species
24    generation, and toxicity. Nano-TiO2 containing more anatase tends to generate more free radicals and
25    induce more toxicity (e.g., cytotoxicity, inflammatory response) than nano-TiO2 containing more rutile
26    (Hidaka et al., 2005; Sayes et al., 2006; Uchino et al., 2002). The influence of crystal forms of nano-TiO2
27    on ROS generation was investigated using a fixed total surface area by Jiang et al. (2008),  who tested 13
28    nano-TiO2 particles of varying crystallinity, all within the size range of 42-102 nm.  Size was found not to
29    affect ROS generation per unit surface area in this study.  The researchers found that the ROS generation


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 1    per unit surface area was highest in amorphous nano-TiO2, followed by anatase and then nano-TiO2
 2    containing both anatase and rutile, and was lowest in rutile nano-TiO2 (Jiang et al., 2008). This finding is
 3    consistent with those of a study investigating unusually fast weathering (loss of gloss) or degradation of
 4    surface coating on steel roofing, associated with sunscreens left by workers during installation (Barker
 5    and Branch, 2008). Nano-TiO2 in the coating-damaging sunscreens was an anatase/rutile mixture,
 6    whereas nano-TiO2 in the one sunscreen that did not accelerate loss of gloss was pure rutile (Barker and
 7    Branch, 2008).
 8          The cytotoxicity of anatase and anatase-mixtures was further increased by UV illumination.
 9    Anatase nano-TiO2 can  be 100 times more cytotoxic under UV than rutile of similar size (Sayes et al.,
10    2006). The hydroxyl (-OH) radical production by nano-TiO2 in cultured cells was found to depend on the
11    crystalline form and size, but differences in OH radical production were not explained by the differences
12    in UV-A absorption between anatase and rutile (Uchino et al., 2002).  Smaller particles that contain more
13    anatase, however, are not always more toxic either in vitro (Sayes et al., 2006) or in vivo (Warheit et al.,
14    2006) than larger particles containing more rutile.

      5.1.1.3. Surface Chemistry
15          Although coatings have been used to decrease the photoreactivity of nano-TiO2 intended for
16    sunscreen (see Section 2.2.2), coatings affect more than photoreactivity. Coatings for nano-TiO2 particles
17    can be designed to reduce agglomeration/aggregation, which in turn affects the behavior of the particles in
18    various media, including sedimentation. This also affects the exposure  to organisms living in different
19    parts of water bodies or feeding on different sized particles. Particle surface modifications can also
20    change the effects of nano-TiO2 on living cells, tissues, or organisms. Using in vitro methods, Serpone et
21    al. (2006) reported that a "thermally assisted" modification of the TiO2 particle surface reduced
22    photocatalytic activity, which in turn decreased (if not eliminated) toxicity to DNA plasmid, human cells,
23    and yeast.  In rats intra-tracheally instilled with two types of nano-TiO2 having the same core material, the
24    nano-TiO2 with a hydrophobic surface (Aeroxide® T805, silanized) caused a slightly lower bioactivity
25    than hydrophilic P25, although the authors concluded that silanization6  did not "lead to remarkable
26    differences in lung reaction" (Rehn et al., 2003).
      6 Silanization is the covering of a surface that has hydroxyl (OH) with molecules that contain only silicon and
      hydrogen (silane), such as SiH4. Silanization is one type of surface modification applicable to particles, such as
      metal oxides, and can render the particle surface chemically inert.

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      5.1.1.4. Recommended Characterization of Nanomaterial for Ecological and
      Toxicological Studies
 1          As noted in Chapter 1, nanomaterials, and nano-TiO2 in particular, can be characterized in several
 2    ways in terms of physicochemical properties (see Table 1-1). Given that the relationship between such
 3    properties and the behavior and effects of nanomaterials, including nano-TiO2, remains to be fully
 4    understood, it might seem desirable for researchers to characterize every possible property of the material
 5    they are investigating. In practice, this is not feasible.  Consequently, recommendations for
 6    characterization of nanomaterials have come forth from time to time.
 7          For in vitro studies, Murdock et al. (2008) recommended characterizing nanomaterial dispersion in
 8    solution for (in no specific order) particle size and size distribution; particle morphology; particle
 9    composition; surface area;  surface chemistry; particle reactivity; agglomeration; zeta potential; and
10    impact of sonication. For "hazard studies with nanoparticle-types", Warheit (2008a) prioritized the
11    characterization needs as (highest priority first): (1) particle size and size distribution (wet state) and
12    surface area (dry state) in the relevant media in the relevant media; (2) crystal structure/crystallinity; (3)
13    aggregation status in the relevant media; (4) composition and surface coatings; (5) surface reactivity; (6)
14    method of nanomaterial synthesisand /or prepration; and (7) purity of sample.
15          An expert working group convened by the International Life  Sciences Institute (ILSI) Research
16    Foundataion/Risk Science Institute recommended measuring mass,  size distribution, surface area, and
17    number for exposure characterization in inhalation studies (Table 5-1), and 17 measurements/aspects for
18    off-line nanomaterial characterization for toxicological studies (Table 5-2) (Oberdorster et al., 2005b).
                       Table 5-1. Recommendations for measuring exposure during
                                 inhalation studies.
Metric Measurement
Mass
Size distribution
Surface area
Number
Recommendation
Off-line
E (coupled with on-line)
E
0
N

On-line
E
E/D
0
E
                       E - These measurements are considered to be essential.
                       D - These measurements are considered to provide valuable information, but are not recommended as
                         essential due to constraints associated with complexity, cost and availability.
                       0 - These measurements are considered to provide valuable but nonessential exposure information.
                       N - These measurements are not considered to be of significant value to inhalation studies.
                       Source: Modified from Oberdorster et al. (2005b)
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Table 5-2. Recommendations for off-line nanomaterial characterization for toxicological studies.
Characterization
Size distribution (primary particles)
Shape
Surface area
Composition
Surface chemistry
Surface contamination
Surface charge -
suspension/solution
Surface charge - powder
(use bio fluid surrogate)
Crystal structure
Particle physicochemical structure
Agglomeration state3
Porosity
Method of production
Preparation process
Heterogeneity11
Prior storage0 of material
Concentration

Toxicity Screening Studies
Human Exposure
Supplied Material
E (combine with
agglomeration state)
E
D
E
D
D
0
0
0
E
E
D
E

D
E
E
E
E
E
E
E
N
E
E
E
E
N
D
E

E
E
--
Administered Material
D
0
D
0
D
D
E
N
0
D
E
N

E
E
E
E
Material
in vivo / in vitro
D
0
0
0
D/0
N
0
0
0
D
D
N


D
--
D
      E - These characterizations are considered to be essential.
      D - These characterizations are considered to provide valuable information, but are not recommended as essential due to constraints associated with
      complexity, cost and availability.
      0 - These characterizations are considered to provide valuable but non-essential information.
      N - These characterizations are not considered to be of significant value to screening studies.
      a As primary particle, secondary particle (primary particle agglomerates and self-assembled structures) and tertiary structure (assemblies of secondary
      strucures). When possible, material agglomeration or de-agglomeration in different liquid media should also be characterized.
      b Time and conditions, including temperature, humidity, exposure to light and atmosphere composition
      c Ratios of different components

      Source: Reprinted from Oberdorster et al. (2005b).

1

2     Three  factors figured into these recommendations:  "the  context within which a material is being

3     evaluated, the importance  of measuring a specific parameter within that context, and the feasibility of

4     measuring the parameter within a specific context" (Oberdorster et al., 2005b).
      5.1.2. Experimental Conditions
5            Experimental conditions, particularly the choice of media/vehicle in which to disperse nano-TiO2,

6     preparation of testing solutions or suspensions, and the formation of aggregates, can influence the

7     behavior and effects of nano-TiO2 and other nanomaterials. The advantages and disadvantages of various
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 1    dispersion preparation methods are compared in a recent publication of nanomaterial ecotoxicity test
 2    methods (Crane et al., 2008).

      5.1.2.1. Media/Vehicle
 3          Nano-TiO2 in an oily dispersion penetrates deeper into skin than nano-TiO2 in an aqueous
 4    dispersion, as shown in an ex vivo study using healthy adult skin (intact samples of tissue removed from
 5    the body, and manipulated in vitro) (Bennat and Muller-Goymann, 2000). Furthermore, when the
 6    dispersal of nano-TiO2 was made in the aqueous phase of an oil-in-water emulsion, nano-TiO2 did not
 7    penetrate into skin, but the emulsion was not stable (Bennat and Muller-Goymann, 2000). Although the
 8    stability could be improved by encapsuling the nano-TiO2 into liposomes, liposome formulation increases
 9    nano-TiO2 skin penetration. An in vivo study by Lansdown and Taylor (1997) in rabbits also
10    demonstrated that uptake of TiO2 particles in sizes ranging from 2 to 20 urn was affected by the vehicle:
11    uptake was greatest in castor oil, followed by water, and then polyethylene glycol.  According to Bennat
12    and Muller-Goymann (2000), the ideal sunscreen formulation, which is stable and does not allow nano-
13    TiO2 penetration into skin, has yet to be developed.
14          Different levels of radical production in cultured cells were observed in similar nano-TiO2 within
15    different formulae of suspensions (Uchino et al., 2002).  Although nano-TiO2 F-1R (a formula containing
16    nano-TiO2 that is 3% anatase and 97% rutile, with an average size of 93 nm and a surface area of 17 m2/g)
17    produced OH radicals  after UV-A exposure, no OH radical production was detected after UV-A exposure
18    in nano-TiO2 in a different formula, St-C n (sunscreen standard C from the Japan Cosmetic Industry
19    Association containing nano-TiO2 that is 2% anatase, 98% rutile, with an average size of 85 nm and a
20    surface area of 19 m2/g). Most rutile nano-TiO2 is relatively inefficient in radical production, and the
21    F-1R used in this study produced more OH radicals than all four other, mainly rutile nano-TiO2 forms and
22    one of the anatase forms tested (Uchino et al., 2002). Although nano-TiO2 has been reported to generate
23    ROS in cell-free conditions but not in cells (a murine macrophage cell line, RAW 264.7) (Xia et al.,
24    2006), whether nano-TiO2 in different formulae also causes different levels of ROS production in cells
25    has not been verified.
26          The purity of water affects the degree of aggregation, which in turn may affect exposure-dose and
27    toxicity. The degree of aggregation generally increases with the presence of salt or with an increase in
28    ionic strength, minerals, and organic matter in water (i.e., decreased purity as compared to pure water)
29    (Domingos et al., 2009a; French et al., 2009). Aggregation was more severe in tap water than in nanopure
30    water (Zhang et al., 2008), and is likely to be more severe in fish tank water or pond water than in tap
31    water.  Because nano-TiO2 in the environment is more likely to be present in aggregated form, results
32    from nano-TiO2 suspensions with aggregates can be informative, and as noted earlier, might even be more

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 1    relevant than results from a perfectly dispersed suspension with nano-TiO2 in primary particle form. The
 2    lack of accurate measurement of nano-TiO2 (e.g., size distribution, mass concentrations, numbers, and
 3    surface area) and a generally-agreed-upon choice of dose metrics, however, impede the establishment of a
 4    reliable dose-response relationship.
 5          In respiratory exposure studies, intra-tracheal instillation exposure typically uses saline as a vehicle
 6    for TiO2 delivery while inhalation exposure uses air. The behavior of nano-TiO2 (such as agglomeration)
 7    is expected to be different in air than in solution. Furthermore, the vehicle alone can affect respiratory
 8    system responses, at least for a short time. Transient inflammation in the respiratory tract occurs in rats
 9    given saline alone through instillation (Driscoll et al., 1990; Henderson et al., 1995). Sager et al. (2007a)
10    tried to disperse several types of nano-sized particles, including TiO2, in several suspension media,
11    including: phosphate buffered saline (PBS); rat and mouse BAL fluid; and PBS containing dipalmitoyl
12    phosphatidylcholine (DPPC) or mouse serum albumin or both. Although the dispersion in PBS was not
13    satisfactory, BAL fluid was an excellent vehicle for dispersing the particles. The dispersion was also
14    unsatisfactory in saline containing albumin alone or DPPC alone, in concentrations found in BAL fluid.
15    Adding protein plus DPPC in PBS, however, produced satisfactory, albeit slightly less effective,
16    substitutes for BAL fluid. The Sager et al. (2007a) experiment demonstrates the importance of the
17    suspension medium and suggests that the immediate milieu (such as the  BAL fluid and protein and DPPC
18    in lung) affects not only the agglomeration of nano-TiO2, but also the consequent effects on nano-TiO2
19    behavior and effects.

      5.1.2.2. Dispersion Preparation
20          The potential importance of dispersion preparation for nanomaterial ecotoxicity is illustrated by
21    fullerene (Ceo) studies.  C60 toxicity in daphnids and fishes was higher when the C60 suspension was
22    prepared with the organic solvent tetrahydrofuran (THF) than when the suspension was prepared by
23    stirring and sonication (Henry et al., 2007; Zhu et al., 2006).  Entrapped or residual THF in the C6o and
24    THF degradation products were suspected to have contributed to toxicity (Henry et al., 2007).
25    Nevertheless, no difference in toxicity to daphnids was observed between nano-TiO2 suspensions
26    prepared with and without THF (Klaper, 2008; Lovern and Klaper, 2006).  Regardless of dispersion
27    method, aggregation of nano-TiO2 might be unavoidable.  Several studies reported that nano-TiO2 formed
28    aggregates in water, and that these aggregates could not be disaggregated into primary particles by
29    ultrasound or chemical dispersants (Griffitt et al., 2008; Jeng and Swanson, 2006; Zhang et al., 2008).
30    Furthermore, an unfiltered nano-TiO2 suspension with aggregates has been reported to be less toxic to
31    daphnia than a filtered nano-TiO2 suspension, which has a much smaller mean secondary particle size
32    than filtered suspension (Lovern and Klaper, 2006). In contrast to the reported difficulty of

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 1    disaggregating secondary particles by sonication or chemical dispersants, Federici et al. (2007) reported
 2    good dispersion of P25 by sonication in ultrapure water at final working concentrations up to 1 mg/L,
 3    although they did not evaluate potential aggregation in test tank water at these concentrations.
 4          In addition to the medium itself, the dispersion method can affect not only the nanoparticle
 5    agglomeration or aggregation (such as the degree and size of agglomerates) but also the effects of
 6    nanoparticles (Bihari  et al., 2008). For example, sonication with ultrasound has been used to decrease
 7    nano-TiO2 agglomeration (Bihari et al., 2008) and has been shown to generate particles or agglomerates
 8    in the nanoparticle range (Maier et al., 2006). However, sonication alone could increase the size of nano-
 9    TiO2 agglomerates, as reported by Porter et al. (2008) who found that the mean agglomerate size of P25
10    in PBS increased from 1930 nm before sonication to 2849 nm immediately after sonication, while the
11    same sonication procedure decreased the sizes of agglomerates of P25 dispersed in BAL fluid and in a
12    mimic BAL fluid that contained Ca2+- and Mg2+- free PBS, serum albumin, and DPPC. No explanation
13    was provided.  Furthermore, ultrasound sonication has been reported to increase nano-TiO2 catalytic
14    activity in breaking down an organic dye (acid red B) (Wang et al., 2009b), but also to decrease changes
15    in enzyme activity caused by ingested nano-TiO2  in isopods (Jemec et al., 2008). Post-preparation
16    analysis of particle size is important when comparing laboratory studies and formulations with sunscreen
17    preparations.  Although studies of nano-TiO2 particle and agglomerate sizes are available (Delrieu et al.,
18    unknown), very few health effects studies have characterized nano-TiO2 in sunscreen formulations and
19    only a few studies characterized nano-TiO2 in other experimental media.  Most health effects studies have
20    reported characteristics of only dry nano-TiO2 primary particles, which are important but not
21    representative of all exposure scenarios.
22          Finally, without a special hydrophilic coating, nano-TiO2 forms a suspension in water (rather than  a
23    solution).  Standard ecotoxicological test methods are intended for soluble or poorly soluble substances,
24    and not designed for testing suspensions (German Federal Institute for Occupational Safety and Health
25    (BAuA) et al., 2007).


      5.1.3. Environmental Conditions
26          Once nano-TiO2 is released into the environment, its fate depends on abiotic and biotic conditions,
27    which are likely to be more complex and diverse than standard ecological testing conditions. Of the many
28    environmental factors that might be relevant to nano-TiO2 ecotoxicity, ultraviolet (UV) exposure, purity
29    of water (Zhang et al., 2008), and presence of organic matter (Domingos et al., 2009a) have been
30    investigated. Factors that affect nano-TiO2 aggregation, such as pH value, ionic strength, and cation
31    valence (Domingos et al., 2009a; Dunphy Guzman et al., 2006; French et al., 2009), would influence not

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 1    only nano-TiO2 fate and transport (see Chapter 3), but also potential exposure and possibly ecological
 2    effects.  Only environmental factors that have been shown to affect toxicity in organisms used for
 3    ecological effects testing are discussed here.
 4          UV is well known to increase the cytotoxicity of nano-TiO2, particularly photocatalytic nano-TiO2
 5    such as anatase or anatase/rutile mix, to cultured mammalians cells (Sayes et al., 2006) and fish cells
 6    (Reeves et al., 2008; Vevers and Jha, 2008) as well as microorganisms (Adams et al., 2006).  Genotoxicity
 7    (Nakagawa et al., 1997) and clastogenicity (Nakagawa et al., 1997; Theogaraj et al., 2007) of nano-TiO2
 8    to cultured mammalians cells were also increased by UV. This UV-increased toxicity is at least partially
 9    due to the greater number of hydroxyl radicals (-OH) generated by anatase than by rutile under UV
10    exposure (Sayes et al., 2006; Uchino et al., 2002).  UV exposure may influence the effects of nano-TiO2
11    in sunscreen indirectly by causing sunburn, which can make skin more permeable (Mortensen et al.,
12    2008).  In addition to UV, visible light was shown to increase the cytoxocity of nano-TiO2 (carbon-doped
13    TiO2 and TiO2 modified with platinum [IV] chloride complexes) in bacteria and fungi (Mitoraj et al.,
14    2007).
15          Nano-TiO2 was found to aggregate more in pond water than in pure water (Milli-Q water),
16    although no nano-TiO2 toxicity to soil bacteria, green algae, or water fleas was detected in either pond
17    water or pure water at up to 100 mg/L (Velzeboer et al., 2008). The adsorption of nano-TiO2 onto
18    certified reference material sediment did not increase the toxicity of the sediment (Blaise et al., 2008).
19          Additional environmental factors that might indirectly influence the effects of TiO2 nanoparticles in
20    sunscreen include moisture; pH and water chemistry; and temperature. High humidity in the environment
21    could increase the hydration level of the  stratum corneum, and could lead to increases in skin
22    permeability and penetration of both hydrophilic and lipophilic components (Benson, 2005; Zimmerer et
23    al.,  1986). For example, the level of penetration of nano-TiO2 on soaked skin, which is likely to occur
24    after swimming or other water activities, has not been investigated. Similar to media and vehicle effects
25    on nano-TiO2, the pH and chemistry of water with which sunscreen may be mixed might also modulate
26    nano-TiO2 effects, e.g., in a pool versus a lake or an ocean.  Finally, sunscreen is often used at much
27    higher temperatures than typical ambient laboratory temperatures. Although nano-TiO2 itself is not
28    expected to change in the temperature range tolerable for human beings, increased body temperature and
29    sweat may affect nano-TiO2 dermal penetration and thus its effects (Lu et al., 2008).
30          The influence of the immediate milieu on nano-TiO2  behavior and effects is also evident when
31    nano-TiO2 is inside an organism. For instance, in vitro studies showed that in rat BAL, nano-TiO2 formed
32    smaller aggregates and the aggregates remained small longer than nano-TiO2 in PBS (Porter et al., 2008;
33    Sager et al., 2007a, b).  Because pH affects the charge of nano-TiO2, it is plausible that nano-TiQ would
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 1    behave differently in tissues and cellular organelles with different pH values, such as very low pH values
 2    in the stomach and in lysosomes.
      5.1.4. Summary
 3          Nano-TiO2 physicochemical properties, experimental conditions, and the immediate environment
 4    or milieu, all can influence nano-TiO2 ecological and health effects.  For example, nano-TiO2 size,
 5    crystalline form, and surface characteristics all influence nano-TiO2 behavior, including distribution,
 6    exposure potential, and effects. Although the influences of media and vehicle and dispersion methods on
 7    particle aggregation and distribution have been reported, information on these influences on health effects
 8    is very scarce (Jemec et al., 2008). The presence of UV and visible light often increase photocatalytic
 9    nano-TiO2 activity and toxicity; other environmental factors, such as pH, ironic strength, and presence of
10    organic matter of the aquatic environment, could also affect nano-TiO2 behavior and effects.
      5.2.  Ecological  Effects
11          The ecological effects of nanomaterials have been gaining attention from the research and
12    regulatory communities, and several review articles (Baun et al., 2008; Boxall et al., 2007; Christian et
13    al., 2008; Hassellov et al., 2008; Navarro et al., 2008; Nowack and Bucheli, 2007; Oberdorster et al.,
14    2006) and conferences (such as the annual International Conference Environmental Effects of
15    Nanoparticles and Nanomaterial) have addressed this topic.  Although new information on nanomaterial
16    ecotoxicity seems to emerge almost daily, available data thus far have been insufficient for a quantitative
17    risk assessment of any particular nanomaterial. Athorough discussion of methods for ecotoxicity testing
18    and characterization of nanomaterials (including in environmental media) is beyond the scope of these
19    case studies, and has been reviewed elsewhere (Christian et al., 2008; Crane et al., 2008; Handy et al.,
20    2008b; Hassellov et al., 2008). Nonetheless, a brief review of ecological effects testing and the
21    importance of the tests are presented at the beginning of each of the following section for the readers'
22    reference.
23          Section 5.2.1 features a review of the ecological effects of nano-TiO2 exposure. Effects on bacteria
24    and fungi are discussed in Section 5.2.1.1, effects on aquatic organisms are discussed in Section 5.2.1.2,
25    effects on terrestrial organisms are discussed in Section 5.2.1.3, and indirect and interactive toxicity are
26    discussed in Section 5.2.2.4. Section 5.2.1.5 summarizes the available ecological toxicity information.
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      5.2.1. Ecological Effects of Nano-TiCh  Exposure
 1          Most of the nano-TiO2 ecological effect studies surveyed in this report (Table 5-3) used
 2    photocatalytic nano-TiO2, some of which could be suitable for water treatment purposes.  Two of the
 3    studies used photostable nano-TiO2 intended for topical sunscreen (Wiench et al., 2007) or for protecting
 4    plastic from UV degradation (Warheit et al., 2007a). Current FDA regulation of TiO2 in topical sunscreen
 5    does not specify crystalline form and does not require proof of photostability (or lack of photoreactivity).
 6    Pure anatase nano-TiO2 is much more photoreactive than pure rutile nano-TiO2, but it is possible to have
 7    photostable anatase or an anatase/rutile mix of nano-TiO2 by using doping or surface treatments, such as
 8    coating with silica. The coating of photostable nano-TiO2 is designed to endure the manufacturing
 9    process and consumer use (Lademann et al., 2000), but the long-term stability of coated TiO2 in sunscreen
10    remains unclear. Once nano-TiO2 is released into the environment, various environmental factors, such as
11    high ionic strength in sea water and high acidity in landfill leachate, could compromise some nano-TiO2
12    coatings. Therefore, the ecological effects of photocatalytic nano-TiO2 might be relevant not only for
13    nano-TiO2 used in drinking water treatment but also for nano-TiO2 in sunscreen, because photoreactive
14    nano-TiO2 can be used as the core material of photostable nano-TiO2 in sunscreen.  For example, the core
15    of Aeroxide T805 is P25, a photocatalyst, and has been used as a UV filter in some sunscreens (Barker
16    and Branch, 2008; Evonik, 2007).
17          Because mass concentration is reported for all studies reviewed, this dose metric is presented in
18    Table 5-3 and in all subsequent discussion referring to the literature. Whenever information on surface
19    area of the particles (to calculate particle surface area concentration) or the measured nano-TiO2
20    concentration (versus calculated based on added mass) in the final test suspension is available, it is also
21    provided in Table 5-3. It should be noted that several studies reported visible turbidity in nano-TiO2 stock
22    suspension (Velzeboer et al., 2008; Zhang et al., 2006;  Zhang et al., 2008).  Because turbidity is likely
23    caused by large aggregates of nano-TiO2, which can settle out of the liquid phase by gravity, actual
24    concentrations of nano-TiO2 in the liquid phase might be lower than concentrations calculated based on
25    mass of nano-TiO2 added.
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 Table 5-3.  Summary of nano-TiOz ecological effects.a
  Test Species
   (Reference)
Material
           Protocol
(No UV illumination, unless specified)
Study Outcome
 Acute Exposure to Microorganisms
Bacteria
(Escherichia coli
and Bacillus
subtilis) (Adams et
al.,2006)
Bacterium ( Vibrio
fischeri) (Blaise et
al.,2008)
Bacterium ( Vibrio
fischeri) (Heinlaan
etal.,2008)
Bacterium ( Vibrio
fischeri) (Velzeboer
etal.,2008)
Bacteria (from a soil
sample, species not
identified)
(Velzeboer etal.,
2008)
Bacteria and yeast
(proprietary
information)
(Blaise etal, 2008;
Dando, 2008)
66-nm powder, -35% rutile:65% anatase, average 330-nm in
water (Sigma product 634662) (Lyon, 2008)
<100-nm powder (Sigma product 634662, Canada or France)
25- to 70-nm powder mixture of anatase and rutile, ratio not
disclosed (Sigma product 13463-67-7, Estonia) (Heinlaan, 2008)
Conventional TiCt size and crystal form not disclosed (Sigma
product 14027, Estonia; a former Riedel-de Haen product)
(Heinlaan, 2008)
<75-nm (primary particle) nano-Ti02 in water suspension (Sigma
product 643017, the Netherlands), mixture of rutile and anatase,
ratio not reported (Velzeboer, 2008)
<75-nm (primary particle) nano-Ti02 in water suspension (Sigma
product 643017, the Netherlands), mixture of rutile and anatase,
ratio not reported (Velzeboer, 2008)
<100-nm powder (Sigma product 634662, France),
characteristics in water not reported
6-hr exposure to (1) 50, 100, 500, 1000, 2000, 500 ppm in
medium11, in direct sunlight,
or (2) 1000 ppm in mediumb, in dark
15-min exposure, measure the reduction of light output from
bioluminescent marine bacterium, Vibrio fischeri (Microtox®
toxicity test) as an indicator of growth inhibition, tested
concentrations not specified
Mix in a 1:1 ratio with certified reference material sediment,
measure light output (Microtox® toxicity test) (indirect
toxi city/interaction)
30 min exposure for up to 20000 mg/L nano-Ti02 and
conventional Ti02, 8 hr exposure to 20000 mg/L conventional
Ti02
Measure the reduction of light output from Vibrio fischeri (flash
assay) as an indicator of growth inhibition
15 min, 1, 10, 100 mg/L, measure light output from
bioluminescent bacteria (Microtox® method, which could be
affected by turbidity of 100 mg/L Ti02 suspension)'
7 day (Biolog®test, gram positive)0, 100 mg/L
18 hr, growth inhibition of 10 bacteria and 1 baking yeast
(microbial array for risk assessment [MARA] assay), tested
concentrations not specified
18-hr exposure to the filtered elutriate from certified reference
material sediment with and without nano-Ti02 mixed in a 1 :1
ratio (MARA assay) (indirect toxicity/interaction) , tested
concentrations not specified
In dark, similar growth inhibition for both bacteria
In light, B. subtilis. 0% and 75% growth inhibition at 500 and
1000 ppm ,b respectively
£ coif. 0%, 15% and 44% inhibition at 100, 500, 1000 ppm,
respectively
IC25>100mg/L
Nano-Ti02 did not affect the toxicity of certified reference
material sediment
The highest concentration tested: 20000 mg/L nano-Ti02 (30 min
exposure) did not decrease bacterial growth
The highest concentration tested: 20 g/L conventional Ti02 (30
min and 8 hr exposure) did not decrease bacterial growth
EC5o>100mg/Lc
EC5o>100mg/Lc
MT0100mg/L
Nano-Ti02 did not affect the toxicity of the elutriate of certified
reference material sediment
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 Table 5-3.  Summary of nano-TiOz ecological effects (continued).
Test Species
(Reference)
Material
Protocol
(No UV illumination, unless specified)
Study Outcome
Acute Exposure to Aquatic Organisms
Alga (green alga,
Desmodesmus
subpicatus) (Hund-
Rinke and Simon,
2006)
Alga (green alga,
Pseudokirchneriella
subcapitata)
(Velzeboeretal.,
2008)
Alga (green alga,
Pseudokirchneriella
subcapitata)
(Warheitetal,
2007a)
Alga (green alga,
Pseudokirchneriella
subcapitata) (Blaise
etal.,2008)
25-nm primary particle, 20% rutile:80% anatase (Degussa P25)
(Baun et al., 2008) (photocatalytic)
100-nm primary particle, 100% anatase; (Hombikat UV100)
(Baun et al., 2008); photocatalytic (Mehrvar et al., 2002)
<75-nm (primary particle) nano-Ti02 in water suspension (Sigma
product 643017, the Netherlands), mixture of rutile and anatase,
ratio not reported (Velzeboer, 2008)
140-nm in water, 79% rutile: 21% anatase, coated (90-wt %
Ti02, 7% alumina, and 1% amorphous silica) (DuPont uf-C TiCy
(photo-passivative/ photo-stable) (Warheit, pers. comm., 2008b)
Fine TiCfe 380-nm in water, rutile, coated (-99% Ti02and -1%
alumina)
<100-nm powder (Sigma product 634662, France),
characteristics in water not reported
72 hr, growth inhibition, following the guidelines for EU standard
algal assay (OECD 201 , DIN 38412-33, and ISO 8692) with
modifications to include pre-illumination of nano-Ti02 dispersion
with simulated sunlight (wavelength 300-800 nm) at 250 watts
for 30 min; illumination alone did not affect D. subspicatus
growth
Algal growth (without preillumiaton): 0, 3.1, 6.2, 12.5, 25, 50
mg/L (producs 1 and 2)
Shading effect:: 0, 12.5,25, 50 mg/L
Algal growth (with preillumiaton): 12.5, 25, 50 mg/L (product 1)
4.5 hr, in light, 100 mg/L
Photosynthesis efficiency was measured as a pulse amplitude
modulation (PAM) fluorescence test, which could be affected by
turbidity of 1 00-mg/L Ti02 suspension c
OECD 201 (72-hr growth), with light"
0.01 , 0.1 , 1 , 10, and 100 mg/L (uf-C Ti02 and fine Ti02)
72-hr growth inhibition, tested concentraions not specified
ECso and effects of additional particle cleaning:
Product 1 : ECso was not different between nano-Ti02 washed
once as manufacturer recommendation (32 mg/L) and nano-
Ti02 with an additional wash (44 mg/L), suggesting toxicity
was not from contaminants
Product 2: ECso >50 mg/L, both nano-Ti02 with and without
the additional wash (at up to 50 mg/L) caused less than 40%
decrease in growth
No shading effect: when nano-Ti02 dispersion (at up to 50
mg/L) was above algae for 72 hrs, no effects on algal growth,
suggesting nano-Ti02 effects was not due to lowered light
intensity, but due to a toxicity of nano-Ti02
Pre-illumination of nano-Ti02 (Product 1) did affect nano-Ti02
effects on algal growth
ECso>100mg/Lc
ECso 21 mg/L (based on decreases in cell number)
ECso 87 mg/L (based on inhibition of growth rate)
ECso 16 mg/L (based on decreases in cell number)
ECso 61 mg/L (based on inhibition of growth rate)
IC25>100mg/L
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 Table 5-3. Summary of nano-TiOz ecological effects (continued).
   Test Species
   (Reference)
                       Material
                       Protocol
        (No UV illumination, unless specified)
                   Study Outcome
 Acute Exposure to Aquatic Organisms (continued)
 Invertebrate (water
 flea, Daphnia
 magna) (Hund-
 Rinke and Simon,
 2006)
25-nm primary particle, 20% rutile:80% anatase (Degussa P25)
(Baun et al., 2008) (photocatalytic); ultrasonic dispersion
                    100-nm primary particle, 100% anatase; (Hombikat UV100)
                    (Baun et al., 2008); photocatalytic (Mehrvar et al., 2002);
                    ultrasonic dispersion
ISO 63421, OECD 202 and DIN 38412-30 (48-hr immobility),
exposure to up to 3 mg/L, 16:8 hr lightdark cycles, compare the
effects of pre-illuminated and non-illuminated nano-Ti02
0,1,1.5, 2, 2.5, 3 mg/L
Pre-illumination increased toxicity compared to the same
concentration
No dose-response relationship with either pre-illuminated or non-
illuminated nano-Ti02
                                                                                                                 Pre-illumination showed a trend of increasing toxicity
                                                                                                                 No dose-response relationship with either pre-illuminated or non-
                                                                                                                 illuminated nano-Ti02
 Invertebrate (water
 flea, Daphnia
 magna) (Lovern
 and Klaper, 2006)
Primary particle <25-nm (smallest 5-nm), anatase, uncoated
(photocatalytic) (Klaper, 2008); filtered through a 0.22-|jm nylaflo
filter, secondary particle 20-30 nm in deionized water
                    Primary particle <25-nm (smallest 5-nm), anatase, uncoated
                    (photocatalytic) (Klaper, 2008); sonicated, unfiltered, secondary
                    particle 100-500 nm in deionized water
EPA 48-hr tox test (U.S. EPA standard operating procedure
2024) (mortality)
Filtered nano-TiCte 0.2,1, 2, 5, 6, 8, and 10 ppm
Sonicated, unfiltered nano-Ti02:50, 200, 250, 300, 400, and 500
ppm
LCso 5.5 mg/L
LOEC 2.0 mg/L
NOEC 1.0 mg/L
                                                                                                                 LCso >500 mg/L
 Invertebrate (water
 flea, Daphnia
 magna) (Wiench et
 al.,2007)
20-30 nm, 80% anatase, 20% rutile, no surface coating, BET
surface area 48.6 m2/g
OECD 202, part 1 (48-hr immobility), tested concentrations: 0
(untreated control), 0.01, 0.1,1.0,10.0 and 100.0 mg/L
EC5o>100mg/L
50-nm x 10-nm, rutile, surface coating aluminum hydroxide,
dimethicone/methicone copolymer, BET 100 m2/g (T-Lite™ SF)
(photostable UV filter)
                                                         EC5o>100mg/L
                    50-nm x 10-nm, rutile, surface coating aluminum hydroxide,
                    hydrated silica, dimethicone/methicone copolymer, BET 100
                    m2/g (T-Lite™ SF-S) (photostable UV filter)
                                                                                                                 EC5o>100mg/L
                    50-nm x 10-nm, rutile, surface coating aluminum hydroxide,
                    hydrated silica, dimethoxydiphenylsilane/triethoxycaprylsilane
                    crosspolymer, BET 100 m2/g (T-Lite™ MAX) (photostable UV
                    filter)
                                                                                                                 EC5o>100mg/L
                    -300-nm, BET surface area 6 m2/g (pigment grade)
                                                                                                                 EC5o>100mg/L
July 2009
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 Table 5-3.  Summary of nano-TiOz ecological effects (continued).
   Test Species
   (Reference)
                       Material
                       Protocol
        (No UV illumination, unless specified)
                   Study Outcome
 Acute Exposure to Aquatic Organisms (continued)
 Invertebrate (water
 flea, Daphnia
 magna) (Lovern et
 al.,2007)
30-nm, anatase
1-hr exposure to 2.0 mg/L
No changes in heart rate or behaviors
 Invertebrate (water
 flea, Daphnia
 magna) (Warheit et
 al.,2007a)
140-nm in water, 79% rutile:21% anatase, coated (90-wt % Ti02,
7% alumina, and 1% amorphous silica) (DuPont uf-C TiCy
(photo-passivative/photo-stable) (Warheit, pers. comm., 2008b)
OECD 202 (48-hr immobility)
0.1,1.0,10, and 100 mg/L (uf-C and fine Ti02)
EC5o>100mg/L
(10% immobility at 100 mg/L)
                    Fine TiCfe ~380-nm in water (buffered), rutile, BET surface area
                    5.8m2/g, coated with alumina (-99% Ti02and~1% alumina)
                                                                                                                 EC5o>100mg/L
                                                                                                                 (10% immobility at 10 mg/L, 0% immobility at 100 mg/L)
 Invertebrates (water
 flea, Daphniapu/ex
 and Ceriodaphnia
 oM>/a)(Griffittetal.,
 2008)
20.5-nm primary particle, mainly220.8-or687.5-nm in
moderately hard water, 20% rutile:80% anatase, BET surface
area 45 m2/g; sonicated (Degussa P25) (photocatalytic)
48-hr mortality, 14:10 hr lightdark cycle, for D. pu/ex adults and
C. dubianeonates (<24 hr old)
Gradient of concentrations up to 10 mg/L (The estimated median
lethal concentration (LCso) from range-finder tests, and 0.6-,
0.36-, 1.67-, and 2.78-fold the estimated LCso. However, the
estimated LCso was not specified.)
LCso >10 mg/L for both D. pu/exand C. dubia
 Invertebrate (water
 flea, Chydorus
 sphaericus)
 (Velzeboeretal.,
 2008)
<75-nm (primary particle) nano-Ti02 in water suspension (Sigma
product 643017, the Netherlands), mixture of rutile and anatase,
ratio not reported (Velzeboer, 2008)
48-hr mortality, 17:7 hr lightdark cycle (Chydotox test)c
ECso>100mg/Lc
 Invertebrates (water
 flea, Daphnia
 magna, fairy
 shrimp,
 Thamnocephalus
 platyurus) (Heinlaan
 etal.,2008)
25- to 70-nm powder mixture of anatase and rutile, ratio not
disclosed (Sigma product 13463-67-7, Estonia) (Heinlaan, 2008)
Conventional TiCt size and crystal form not disclosed (Sigma
product 14027, Estonia; a former Riedel-de Haen product)
(Heinlaan, 2008)
48-hr mortality for D. magna
24-hr immobilization for T. platyurus
Up to 20000 mg/L for both nano- and conventraional TiCfe
NOEC >20,000 mg/L for T. platyurus, not tested in D. magna
                                                        NOEC >20,000 mg/L for T. platyurus, 60% mortality at 20,000
                                                        mg/L for D. magna
 Invertebrate (fairy
 shrimp,
 Thamnocephalus
 platyurus) (Blaise et
 al.,2008)
<100-nm powder (Sigma product 634662, France),
characteristics in water not reported
24-hr lethality (ThamnoToxkit assay), tested concentrations not
specified
LCso > 100 mg/L
July 2009
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 Table 5-3.  Summary of nano-TiOz ecological effects (continued).
   Test Species
   (Reference)
                     Material
                     Protocol
        (No UV illumination, unless specified)
                  Study Outcome
 Acute Exposure to Aquatic Organisms (continued)
Invertebrate
(freshwater hydra,
Hydra attenuate)
(Blaiseetal.,2008)
Fish cell (trout
primary
hepatocytes)
(Blaiseetal.,2008)
Fish (zebrafish,
Daniorerio),
embryo and larvae
(Zhuetal.,2008)
Fish (zebrafish,
Danio re/vb) (Griffitt
etal.,2008)
Fish (rainbow trout,
Oncorhynchus
mykiss) (Warheit et
al.,2007a)
<100-nm powder (Sigma product 634662, France),
characteristics in water not reported
<100-nm powder (Sigma product 634662, France),
characteristics in water not reported
Nano-TiCfe uncoated anatase, purity >99.5%, primary particle in
spindle shape, published size s20 nm, surface area not reported
(Nanjing High Technology NANO CO., LTD, Nanjing, Jiangshu
province, China); in suspension (in MilliQ water): mean
measured size 230 nm, measured size range 100-550 nm,
secondary particles formed by primary particles have irregular
shapes
Conventional TiCt anatase, purity >99.0%, published size:
10,000 nm (Third Chemical Regent Factory of Tianjin.Tianjin,
China); in suspension (in MilliQ water): mean measured size
1 , 1 00 nm , measured size range 330-2,250 nm , neither primary
nor secondary particles have a uniform shape
20.5-nm primary particle, mainly220.8-or687.5-nm in
moderately hard water, 20% rutile:80% anatase, BET surface
area 45 m2/g, sonicated (Degussa P25) (photocatalytic)
140-nm in water, 79% rutile:21% anatase, coated (90-wt % Ti02,
7% alumina, and 1% amorphous silica) (DuPont uf-C TiCy
(photo-passivative or photo-stable) (Warheit, pers. comm.,
2008b)
96-hr morphological changes, tested concentrations not
specified
48-hr cytotoxicity, tested concentrations not specified
96-hr exposure to 0, 1, 10, 50, 100, or 500 mg/L nano-Ti02 or
conventional Ti02 to fish eggs (started within 1 .5 hr post-
fertilization); light cycle 14 hr light/10 hrdark; following endpoints
were measured:
(1) survival of embryo and larvae
(2) hatching rate at 84 hr post-fertilization
(3) malformation (e.g., pericardial edema and tissue
ulceration, body arcuation, etc.) in embryo and larvae
48-hr mortality on adult zebra fish and zebra fish fry (<24 hr
post-hatch) at a gradient of concentrations up to 10 mg/L
OECD 203 (96 hr)
0.1, 1.0, 10, and 100 mg/L (uf-C and fine Ti02)
ECso in 10-1 00 mg/L range
TEC in 1-10 mg/L range
Neither nano-Ti02 nor conventional Ti02 at the tested condition
caused changes in any of the three endpoints measured.
LCso>10 mg/L for both adults and fry
LC5o> 100 mg/L
 Chronic Exposure to Aquatic Organisms
 Invertebrate (water
 flea, Daphnia
 magna) (Adams et
 al.,2006)
66-nm powder, -35% rutile:65% anatase, average 330 nm in
water, (Sigma product 634662) (photocatalytic) (Lyon, 2008)
8-day exposure to suspension at 1,10 or 20 ppm (concentration
over time was not reported)
40% mortality at 20 mg/L
 Invertebrate (water
 flea, Daphnia
 magna) (Wiench et
 al.,2007)
50-nm x 10-nm, rutile, surface coating aluminum hydroxide,
hydrated silica, dimethicone/methicone copolymer, BET surface
area 100 m2/g (T-Lite™ SF-S) (photostable UV filter)
OECD 211 (21-d reproduction), test concentrations:  0.01,0.03,
0.1,0.3,1,3,10,30,100 mg/L
NOEC 3 mg/L
LOEC10mg/L
July 2009
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 Table 5-3.  Summary of nano-TiOz ecological effects (continued).
   Test Species
    (Reference)
                        Material
                        Protocol
        (No UV illumination, unless specified)
                    Study Outcome
 Chronic Exposure to Aquatic Organisms (continued)
 Fish (rainbow trout,
 Oncorhynchus
 mykiss) (Federici et
 al.,2007)
21-nm, 75% rutile:25% anatase, sonicated (Degussa P25)
(photocatalytic)
0-, 7-, or 14-day exposure to 0, 0.1, 0.5 or 1.0 mg/L(mean
measured
Ti02 concentrations were 0.089, 0.431, and 0.853 mg/L over the
12-hr period, equating to 89, 85, and 86% of the expected
concentrations, respectively)
Respiratory distress, organ pathologies, and oxidative stress at
as low as 0.1 mg/L; nano-TiCfe could be a surface acting toxicant
 Acute Exposure to Terrestrial Organisms
 Photosynthetic
 enzyme complexes
 isolated from
 spinach leaves
 (Blaiseetal.,2008)
<100-nm powder (Sigma product 634662, Canada or France),
characteristics in water not reported
15 min, tested concentrations not specified, measure the
decrease in chlorophyll fluorescence emitted from the enzyme
complexes as an indicator of inhibition of phytosynthetic
efficiency (Luminotox assay) (Bellemare et al., 2006)
                                                                              Mix in a 1:1 ratio with certified reference material sediment, 15
                                                                              min, tested concentrations not specified, measure light output
                                                                              (Luminotox assay) (indirect toxicity/interaction)
IC2o>100mg/L
                                                                                                                   Nano-Ti02 did not affect the toxicity of certified reference
                                                                                                                   material sediment
 Plant (spinach,
 Spinacia oleracea)
 (Linglan etal.,
 2008)
Nano-TiCt 5-nm, anatase, not coated
Conventional Ti02
Soak the seeds in 0.25% nano-Ti02 or conventional Ti02 for 48
hr, and spray 0.25% nano-Ti02  or conventional Ti02 onto the
leaves from 2-leaf stage to 8-leaf stage at 0.25%
Nano-TiCfe
  Enhanced growth (size, single plant fresh weight, single plant
  dry weight)
  Increased chlorophyll content
  Increased net photosynthetic rate
  Increased mRNA, protein concentration, and activity of
  Rubisco activase
Conventrional TiCt
  No significant changes
 Plant (spinach,
 Spinacia oleracea)
 (Zheng etal.,2005)
Size not specified, rutile (Shanghai Chemical Co. of China
product)
Soak aged seeds for 48 hr at 0, 0,25, 0.5, 1.0,1.5, 2.0, 2.5, 4.0,
6.0, or 8.0 mg/L
Increased germination rate, intensity of photosynthesis,
chlorophyll synthesis, and Rubisco activase activity in a dose
response manner (at up to -4.0 mg/L; peak effect at ~2 mg/L;
higher concentrations have opposite effects)
 Invertebrate
 (isopod, Porcellio
 scaber) (Jemec et
 al.,2008)
15-nm in diameter, 15-75 nm in length, elongated spheroid
shape, anatase, surface area 190-290 m2/g, 99.7% pure (Sigma
product).  350- to 500-nm aggregates in sonicated dispersion,
780- to 970-nm aggregates in non-sonicated dispersion, sizes on
dry leaves not reported
3-day dietary exposure to non-sonicated nano-TiCfe at 0.1, 0.5,1,
10,100,1,000, 2,000, or 3,000 pg/g food or to sonicated nano-
Ti02 at 1,000, 2,000, or 3,000 pg/g food (leaves soaked in non-
sonicated or sonicated nano-TiCh dispersion and then dried)
Decreased activities of catalase and glutathione-S-transferase
(GST) in digestive glands at 0.5, 2,000, and 3,000 pg/g non-
sonicated nano-Ti02, but not in middle doses of non-sonicated
nano-Ti02 or any doses of sonicated nano-Ti02
July 2009
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 Table 5-3.  Summary of nano-TiOz ecological effects (continued).
   Test Species
   (Reference)
                       Material
                       Protocol
        (No UV illumination, unless specified)
                   Study Outcome
 Acute Exposure to Terrestrial Organisms (continued)
 Invertebrate
 (nematode,
 Caenorhabditis
 elegans) (Wang et
 al.,2009a)
Nano-Ti02, anatase, primary particle diameter 50 nm, measured
BET surface area 325 m2/g for primary particle, purity >99%,
hydrodynamic diameter (of aggregates in pure water) range
338-917 nm (medium 550 nm), zeta potential at pH 7.0 = -18.9
mv (Hongchen Material Sci & Tech, Co., China)
Conventional Ti02, anatase, measured primary particle diameter
285 nm (by TEM), measured BET surface area 7.3 m2/g, purity
>99%, hydrodynamic diameter range 158-687 nm (medium 494
nm), zeta potential at pH 7.0 = - 33.8 mv (ACROS)
Expose synchronized worms in the L1 stage to nano-Ti02 or
conventional Ti02 in ultrapure water with pH adjusted to 7.0 with
HN03 and NaOH
Exposure for 24 hr (for lethality to the vermiform nematode) or 5
days (for growth - length of the worm, and reproduction tests -
number of eggs inside the worm body, and number of offspring
per worm) at 24.0, 47.9,95.9,167.8, and 239.6 mg/L
Lethality to the vermiform nematode:  24-hr LCso was
significantly lower for nano-Ti02 (79.9 mg/L) than for
conventional Ti02 (135.8 mg/L)
Length of the worm,  number of eggs inside the worm body, and
number of offspring per worm were all significantly decreased at
47.9 mg/L or higher concentrations of nano-Ti02 and at 95.9
mg/L or higher concentrations of conventional Ti02
  N/A - Not applicable
  ACROS - Acros Organics
  BET - Surface area measured by Brunauer, Emmett, and Teller analysis
  DIN - Deutsches Institut fur Normung (German Institute for Standardization)
  ECso - Effective  concentration 50; the concentration at which 50% of subjects showed response
  EU-European Union
  ICai, IC25-inhibitory concentration at which organisms showed 20%, 25% inhibition in measured endpoints
  ISO - International Organization for Standardization
  GST - Glutathione-S-transferase
  LCso - Lethal concentration 50; the concentration at which 50% of subjects died
  LOEC - Lowest  observed effect concentration
                                                                           MARA - Microbial array for risk assessment (assay)
                                                                           MTC - Microbial Toxic Concentration, calculated by comparing the area under and above the growth curve
                                                                               (Gabrielson et al, 2003a, 2003b)
                                                                           NOEC - No observed effect concentration
                                                                           OECD - Organization for Economic Co-operation and Development
                                                                           P25-AEROXIDEOP25
                                                                           RAM - Pulse amplitude modulation
                                                                           TEC - Threshold effect concentration. The TEC for cytotoxicity is calculated using the NOEC and LOEC of
                                                                               cell viability reduction. TEC = (NOEC x LOEC)1'2
                                                                           TEM - Transmission electron microscopy
                                                                                              UV-Ultraviolet (light/radiation), wavelengths in the range of 10-400 nm
  Authors reported cloudy appearance or difficulty to dissolve nano-Ti02 in preparing stock suspension. The testing concentrations (final concentrations in medium) were calculated by the volume of 10 mg/L stock
  suspension added into the medium.  The actual concentrations of nano-Ti02 in medium were not reported.
  Authors reported cloudy appearance in 100 mg/L Ti02suspension. After centrifugation,  nano-Ti02 concentrations were no more than 10% of initial concentrations.  For example, 200 pg/L nano-Ti02 was added into pond
  water, and nano-Ti02was only 1 pg/L after centrifugation.
July 2009
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      5.2.1.1. Effects on Bacteria and Fungi (Terrestrial and Aquatic)
 1          Data for the effects of photostable nano-TiO2 on bacteria and fungi are lacking.  On the other hand,
 2    photocatalytic nano-TiO2 is known for its antibacterial and antifungal properties and has been tested for
 3    various applications, including drinking water treatment (Coleman et al., 2005); surface coatings and
 4    paints (Kiihn et al., 2003; Tsuang et al., 2008); and food packaging (Chawengkijwanich and Hayata,
 5    2007). Examples of recent studies of photocatalytic nano-TiO2 in bacteria and fungi are provided in
 6    Table 5-3.
 7          Because most bacteria and fungi are non-pathogenic and are major decomposers in most terrestrial
 8    and some aquatic ecosystems, chemicals with antibacterial and antifungal properties are not necessarily
 9    beneficial when released into the environment.  The health of decomposers is important for nutrient
10    cycling in the environment, such as carbon and nitrogen cycling in soil (Neal, 2008). Additionally, some
11    bacteria and fungi form a symbiotic relationship with plants. A well-known example is the nitrogen-
12    fixing bacteria (genus Rhizobium) that live in the roots of legumes.  Legumes provide nutrients and a
13    relatively anaerobic environment for the  rhizobia, and obtain ammonia formed from atmospheric nitrogen
14    by the rhizobia (Kimball, 2007). Thus, indiscriminant exposure to chemicals with antibacterial properties
15    could harm plants by interfering with symbiotic bacteria.
16          Sensitivity to photocatalytic nano-TiO2 toxicity varies among species of bacteria. Adams et al.
17    (2006) reported that in the presence of sunlight, gram-negative Escherichia coll were more sensitive to
18    nano-TiO2-induced growth inhibition than gram-positive Bacillus subtilis. With 2,000  parts per million
19    (ppm) of nano-TiO2 in the growth medium, E. coll. growth was decreased by 46% while B. subtilis
20    growth was inhibited by 99%.  At 500 ppm, E. coll. growth was  decreased by only 15% and B. subtilis
21    growth was not inhibited (Adams et al., 2006).  The different dose-response relationships of gram-positive
22    and gram-negative bacteria to nano-TiO2 suggests the  potential for nano-TiO2 to alter microbial
23    population balance (diversity), both in wastewater treatment plants and during various  phases of use and
24    disposal of nano-TiO2.  One  generally accepted explanation for nano-TiO2-induced toxicity in bacteria
25    and fungi is the generation of ROS, which can cause cell wall or cell membrane damage (Kiihn et al.,
26    2003; Neal, 2008), such as lipid peroxidation (Maness et al., 1999). Although, as discussed above, UV
27    illumination increases photocatalytic nano-TiO2 toxicity, photocatalytic nano-TiO2 is also toxic in the dark
28    (Adams et al., 2006; Coleman et al., 2005). Because TiO2 generates ROS (mainly highly reactive
29    hydroxyl radicals, -OH) in the presence of UV and oxygen (Reeves et al., 2008), mechanisms other than
30    oxidative stress might also contribute to nano-TiO2 toxicity in the dark  and possibly also under UV.  For
31    example, several types of nano-TiO2 (anatase and a mixture of anatase/rutile) have been shown to adsorb
      July 2009                                        5-20                      DRAFT-DO NOT QUOTE OR CITE

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 1    protein and calcium (Ca2+) in the medium, and cause in vitro cytotoxicity in mammalian cell lines (Horie
 2    et al., 2009).

      5.2.1.2. Effects on Aquatic Organisms
 3          Data on the effects of nano-TiO2 in aquatic organisms are available for freshwater algae, freshwater
 4    invertebrates (water fleas and fairy shrimp), and freshwater fish (rainbow trout) (Table 5-3). Only two
 5    aquatic organism studies in the literature involve photostable nano-TiO2 (Warheit et al., 2007b; Wiench et
 6    al., 2007). For other aspects of U.S. Environmental Protection Agency (EPA) tier 1 aquatic toxicity
 7    testing (e.g., estuarine and marine organism acute toxicity, whole sediment acute toxicity, and bio-
 8    availability/bio-magnification toxicity) (U.S. EPA, 2008d), studies have not yet been reported.

 9    5.2.1.2.1. Algae
10          Algae are primary producers in ecosystems. In addition to being the food base in aquatic systems,
11    algae provide much of the earth's oxygen.  Effects on algae are measured at the population level, for
12    example, in terms of population growth.  In algal tests, 72-hour exposures are considered acute exposure
13    in European Union (EU) regulations, and 96-hour exposures are considered chronic by U.S. EPA (2008d).
14    A limited number of studies on the effects of either photocatalytic or photostable TiO2 in algae have been
15    completed.
16          For photostable nano-TiO2, EC50 values determined for 72-hour growth inhibition in green alga
17    (Pseudokirchneriella subcapitata) were 21  mg/L (based on decreases in healthy cell numbers) and 87
18    mg/L (based on inhibition of growth rate) (Warheit et al., 2007a). In contrast, exposure to concentrations
19    of 0.001 to 1 mg/L of photostable nano-TiO2 increased growth rate by 1-3% (green alga cell numbers
20    increased 6-19%) (Warheit et al., 2007a). U-shaped dose-response relationships are not unique to
21    nanomaterials, and it cannot be ruled out that increased growth at the low dose  was a compensatory
22    response to low levels of toxicity (Calabrese and Baldwin, 1998; Davis and Svendsgaard,  1990). Fine
23    (approximately 380-nm) TiO2 showed almost no inhibition in growth rate (or cell number) at up to
24    1 mg/L, and inhibition of growth rate was 3% at 10 mg/L and 66% at 100 mg/L (Warheit et al., 2007a).
25          For photocatalytic nano-TiO2, the EC50 values determined for 72-hr growth inhibition in green
26    algae (Desmodesmus subpicatus) ranged from approximately 30 mg/L to more  than 50 mg/L (Blaise et
27    al., 2008; Hund-Rinke and Simon, 2006). Hund-Rinke and Simon (2006) also  tested the potential for
28    TiO2 to reduce growth by physically shading algae, and reported that as much as 50 mg/L of
29    photocatalytic nano-TiO2 physically above  the algae did not decrease algal growth, that is, it did not cause
30    a shading effect.  When nano-TiO2 and algae are in the same liquid medium, photocatalytic P25 nano-
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 1    TiO2 was reported to adsorb onto the surfaces of green algae (Pseudokirchneriella subcapatitata) and to
 2    increase cellular weight by more than 130% (Huang et al., 2005).  The concentration of P25 was not
 3    reported. If the attached nano-TiO2 directly blocks sunlight that otherwise could reach the algal cell
 4    surface or if this extra weight causes algae to stay in deeper water, the consequent reduction in sunlight
 5    could inhibit the algal growth.  Because photostable nano-TiO2 would also block UV penetration, similar
 6    effects could occur with photostable nano-TiO2. Without experimental evidence,  predicting the impact of
 7    nano-TiO2 on photosynthesis is difficult because nano-TiO2 exposure reportedly increases photosynthesis
 8    in terrestrial plants, namely spinach, as discussed later in this section. Nano-TiO2 could affect aquatic and
 9    terrestrial plants differently due to exposure routes, doses, and other factors.
10          Although no marine organisms have been tested for nano-TiO2 toxicity, the physical attachment of
11    nano-TiO2 particles on cells could pose a risk to aquatic organisms that reproduce by external fertilization.
12    A wide variety of marine organisms fall into this category. Attached nano-TiO2 could decrease sperm cell
13    mobility and consequently reproductive success. For comparison, carbon black nanoparticles have been
14    reported to decrease sperm frequency of seaweed (marine macroalgae) and to affect seaweed embryo
15    development (Nielsen et al., 2007). As discussed earlier (Section 5.1.1), the salinity in seawater could
16    influence the behavior and effects of nano-TiO2, such as more aggregation as compared to pure water.
17          Nano-TiO2 was reported to increase algal cell weight 2.3-fold by adsorbing to the algal cell surface,
18    but the tested nano-TiO2 concentrations in water were not reported (Huang et al., 2005). If an increase in
19    weight forces surface algae into deeper water, photosynthesis could be decreased7 due to less sunlight
20    available in deeper water than at the surface. Because phytoplankton form the base of the food web and
21    generate half of the oxygen produced by all plants (Ramanujan, 2005), harmful effects on phytoplankton
22    from nano-TiO2 could have wide-ranging  implications.

23    5.2.1.2.2. Invertebrates
24          The endpoints used  most often in ecological studies with invertebrates are mortality and
25    immobility; other endpoints include morphological changes, heart rate changes, and reproductive effects.
26    Fairy shrimp, Thamnocephalus platyurus, are small freshwater crustaceans and filter feeders that live in
27    temporary water bodies that dry out or periodically experience decreased water levels (Brausch et al.,
28    2006; Lohr et al., 2007). In the dry season, T. platyurus survives by laying resting-stage eggs (known as
29    cysts), which hatch into nauplii (first stage of crustacean larvae) within hours after being hydrated
30    (Brausch and Smith,  2009). The lethality  and immobilization in T. platyurus larvae and adults as well as
31    the hatch rate of T. platyurus cysts are often used as endpoints for freshwater contaminant tests. Hydras
      7 On the other hand, nano-TiO2 taken up by spinach increased growth and photosynthesis by increasing the activities
       of enzymes important for photosynthesis (Linglan et al., 2008; Zheng et al., 2005).

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 1    (Hydra attenuatd) are small simple animals with a tube-shape body (usually 1-20 mm long) and tentacles
 2    on one end of the body. Intoxication of hydras can be seen in tentacle morphology, which can be normal,
 3    clubbed (a sign of minor intoxication), shortened (severe intoxication), or completely retracted (lethal
 4    intoxication, because this inevitably leads to death) (Environment Canada, 2007).
 5          Acute and chronic toxicity of nano-TiO2 intended for sunscreen use was studied in Daphnia magna
 6    and reported in a poster at a scientific meeting by Weinch et al. (2007). In the acute exposure study, EC50
 7    values (from 48-hour mortality tests) were above 100 mg/L for all tested forms of TiO2, which consisted
 8    of three  photostable forms (uncoated T-Lite™ SF, coated T-Lite™ SF-S, and coated T-Lite™ MAX), a
 9    photocatalytic nano-TiO2, and a pigment-grade TiO2  (Wiench et al., 2007).  In the chronic exposure study,
10    photostable coated T-Lite SF-S was given to Daphnia magna at up to 100 mg/L for 21  days, and the LC0
11    was 30 mg/L.  In this study, death was determined by the lack of swimming ability.
12          For reproductive effects after 21 days, the no observed effect concentration (NOEC) value for T-
13    Lite SF-S was 3 mg/L, and the lowest observed effect concentration (LOEC) value was 10 mg/L (Wiench
14    et al., 2007). In a different study that used photostable nano-TiO2 intended to protect plastics against UV-
15    induced degradation, 48-hr exposure to 100 mg/L of the nano-TiO2  induced 10% immobility in Daphnia
16    magna (Warheit et al.,  2007a).
17          The effects of photocatalytic nano-TiO2 toxicity have been studied by several research teams in
18    four types of water fleas (Daphnia magna, Daphnia pulex, Ceriodaphnia dubia, and Chydorus
19    sphaericus), one type of fairy shrimp (T. platyurus), and one type of freshwater hydra (Hydra attenuatd).
20    For water fleas, the 48-hour mortality or immobility EC50 was generally greater than 100 mg/L (Lovern
21    and Klaper, 2006; Velzeboer et al., 2008; Wiench et al., 2007), with two exceptions. One study reported
22    an LC50  greater than 10 mg/L, which in this case was the highest concentration tested (Griffitt et al.,
23    2008). Another study reported a 48-hour LC50 of 5.5 mg/L, using filtered nano-TiO2 samples, which have
24    an average particle size of 30 nm after going through a 0.22-mm Nylaflo  filter (Lovern and Klaper, 2006).
25    In contrast, unfiltered nano-TiO2 samples had all sizes of nano-TiO2 clumps, ranging from 100 to 500 nm
26    in diameter, and the mortalities never exceeded 11%  at up to 500 mg/L (Lovern and Klaper, 2006).
27    Chronic exposure for 8 days caused 40% mortality at 20 mg/L in daphnids (Adams et al., 2006).  For fairy
28    shrimp, the 24-hr mortality or immobility LC50 was higher than 100 mg/L (Blaise et al., 2008; Heinlaan et
29    al., 2008). In the only  study of hydra, the EC50 of 96-hour morphological changes was less than 100 mg/L
30    (Blaise et al., 2008). The relative sensitivity among these aquatic invertebrates to nano-TiO2 cannot be
31    determined, due to the  variability of tested nano-TiO2 formulations and experimental designs.
32          When Daphnia magna were exposed to photocatalytic P25 nano-TiO2 in water, nano-TiO2 was
33    observed on the exoskeleton and antennae and in the  digestive tract (Baun et al., 2008). Baun et al.
34    (2008) noted that the aggregation of nanoparticles on the exoskeleton, at sufficient dose, might impede a

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 1    daphnid's mobility. Although not investigated in this study, the aggregation of nanoparticles on the
 2    antennae, a chemosensory organ important for feeding and reproductive behaviors, could adversely affect
 3    a daphnid's growth and reproduction (Oberdorster et al., 2006). Because nano-TiO2 primary particles are
 4    smaller than the size range of particles daphnids feed on (400-40,000 nm), the presence of nano-TiO2 in
 5    the digestive tract suggests that daphnids feed on nano-TiO2 aggregates (Baun et al., 2008). Whether
 6    nano-TiO2 is taken up by other tissues, excreted, or transformed in daphnids is unclear (Baun et al., 2008).
 7    Even if nano-TiO2 is not absorbed into tissues, nano-TiO2 in the digestive tract of daphnids could still
 8    contribute to bioaccumulation in the food web (see Section 4.4.3).
 9          The behavior and heart rate ofDaphnia magna were evaluated in daphnids exposed to
10    photocatalytic nano-TiO2 at 2.0 mg/L for 1 hour (Lovern et al., 2007).  In this study, nano-TiO2 had an
11    average particle diameter of 30 nm, and tetrahydrofuran, an organic solvent used to prevent aggregation,
12    was not detected in the final nano-TiO2 suspension. The concentration of 2.0 mg/L was selected because
13    it was the lowest observed effect level (LOEL) ofDaphnia magna mortality after 48-hour exposure
14    (Lovern and Klaper, 2006).  Behavior (e.g., hopping frequency, appendage movement as an indicator of
15    feeding frequency, and postabdominal claw curling) and heart rates were not affected by the 1-hour nano-
16    TiO2 exposure (Lovern et al., 2007).

17    5.2.1.2.3. Fish
18          Fish are used in ecological tests to represent secondary consumers in aquatic systems. Commonly
19    used fishes in ecological tests include freshwater species rainbow trout (Oncorhynchus  mykiss), bluegill
20    sunfish (Lepomis macrochirus), fathead mirmows,(Pimephales promelas) and estuarine species
21    sheepshead minnows (Cyprinodon variegatus). Data from zebra fish (Danio rerio), a model organism
22    widely used in biological and toxicological  studies, can also be useful. Fish study endpoints can include
23    concentrations of chemicals,  such as in fish bioaccumulation tests (see Section 4.4.1.1,  Exposure);
24    mortality; behavioral markers (e.g., coughing and swimming); and pathology.
25          The toxicological studies of photostable nano-TiO2 in fish are very limited. The  96-hr acute
26    toxicity of photostable nano-TiO2 (DuPont uf-C) in rainbow trout produced an LC50 value of greater than
27    100 mg/L (Warheit et al., 2007a). However, DuPont uf-C is designed to protect plastics from UV-induced
28    degradation, and is not known to be used in sunscreen; no fish studies of nano-TiO2 intended for
29    sunscreen use were found.
30          In contrast, photocatalytic nano-TiO2, which may be used in drinking water treatment, has been
31    tested in fish for acute effects (Griffitt et al., 2008; Zhu et al.,  2008) and chronic effects (Federici et al.,
32    2007) (see following discussion), as well as bioaccumulation  (Zhang et al., 2006) and interaction with
33    other heavy metals (see Section 4.2, Exposure).  In the acute exposure study, the LC50 for a 48-hr

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 1    exposure to an anatase/rutile mixture of uncoated nano-TiO2 was greater than 10 mg/L for zebrafish (in
 2    both female adults and <24-hr post-hatch fry) (Griffitt et al., 2008).  For zebrafish eggs (blastula stage),
 3    acute exposures for 96 hours at up to 500 mg/L of either nano-TiO2 or conventional TiO2 (both uncoated
 4    anatase) did not cause developmental toxicity, as measured by survival rate of the zebrafish embryos and
 5    larvae, hatching rate of embryos, and malformation in embryos and larvae (Zhu et al., 2008). In the Zhu
 6    et al. (2008) study, nano-A!2O3 and conventional A12O3 at up to 1000 mg/L also did not cause
 7    developmental toxicity to zebrafish eggs, but both nano-ZnO and conventional  ZnO caused decreases in
 8    survival rates and hatching rate as well as increases in tissue ulceration at 1 mg/L or higher
 9    concentrations.
10          Sub-lethal toxicity was observed in juvenile rainbow trout after 14 days of exposure to
11    photocatalytic P25 nano-TiO2 (Federici et al., 2007). Respiratory toxicity and pathological changes in the
12    gill and intestine were seen after a 14-day exposure at concentrations as low as  0.1 mg/L.  Furthermore,
13    there were signs of oxidative stress (increased concentrations of thiobarbituric acid  substances, an
14    indicator of lipid peroxidation and oxidative stress, in multiple tissues), and activation of anti-oxidant
15    defenses (increased total glutathione levels in the gill). Na+K+-ATPase activity was also increased in the
16    gill and intestine. Disturbances were observed in the metabolism of copper and zinc, but not of Na+, K+,
17    Ca2+orMn. No major hematological disturbances were observed. Worth noting  is  that these effects
18    occurred without appreciable titanium accumulation in the internal organs, suggesting no nano-TiO2
19    accumulation, as discussed earlier in Section 4.4.1.  The authors suggested that surface-bound TiO2
20    (through surface adsorption) might play a role in toxicity, similar to the case of aluminum, a surface-
21    acting toxicant that can cause systemic toxicity without significant internal accumulation. Federici et al.
22    (2007) concluded that although nano-TiO2 was not a major hemolytic toxicant or disrupter of ion
23    regulation in this study, respiratory distress, organ pathologies, and oxidative stress  were  adverse effects.

24    5.2.1.2.4. Summary of Effects on Aquatic Organisms
25          Sub-lethal effects of nano-TiO2 include decreases in daphnid reproduction by photostable nano-
26    TiO2 (Wiench et al., 2007), as well as respiratory distress, pathological changes in gills and intestine, and
27    behavioral changes in fish (rainbow trout) by photocatalytic nano-TiO2 (Federici et al., 2007).  Several
28    studies reported visible turbidity in nano-TiO2 stock suspensions, and the actual nano-TiO2 concentration
29    in the liquid phase might be different from the concentration calculated from added nano-TiO2 (Velzeboer
30    et al., 2008; Zhang et al., 2006; Zhang et al., 2008). Given that natural organic  matter in the environment
31    can induce aggregation and deposition of nanoparticles or modify nanoparticle  surface charges (Navarro
32    et al., 2008), the bioavailability and behavior of nano-TiO2 in the environment are likely to be different
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 1    from bioavailability and behavior in pure water or simple media, although the direction of the difference
 2    is difficult to predict.

      5.2.1.3. Effects on Terrestrial Organisms

 3    5.2.1.3.1. Plants
 4          Information on nano-TiO2 interactions with plants is available only for photocatalytic uncoated
 5    nano-TiO2 in spinach (Table 5-1).  Photocatalytic uncoated nano-TiO2 has been shown to enhance the
 6    growth of spinach in several studies (Lei et al, 2008; Linglan et al, 2008; Mingyu et al., 2007a; Mingyu
 7    et al., 2007b; Yang et al., 2006; Zheng et al., 2005).  When a nano-TiO2 suspension was used to soak the
 8    seeds and was sprayed on the leaves, the germination rate and growth of the plant were enhanced (Zheng
 9    et al., 2005). These  effects were at least partially due to nano-TiO2-induced increases in the activity of
10    several enzymes important for photosynthesis (Linglan et al., 2008), adsorption of nitrate, transformation
11    of inorganic into organic nitrogen (Yang et al., 2006), and anti-oxidative stress response (Lei et al., 2008).
12    Conventional TiO2 suspensions showed either insignificant effects (in comparison with untreated
13    controls) or much smaller effects than nano-TiO2 did (Linglan et al., 2008; Zheng et al., 2005).

14    5.2.1.3.2. Invertebrates
15          The only known studies on the effects of nano-TiO2 on terrestrial  invertebrates include a study on
16    an isopod, Porcellio scaber (Jemec et al., 2008), and a study on nematodes, Caenorhabditis elegans
17    (Wang et al., 2009a). Living in soil, isopods and nematodes contribute to nutrient cycling and
18    decomposition, and have been used as indicators of soil pollutants.
19          Jemec et al. (2008) investigated the effects of photocatalytic anatase nano-TiO2 on the terrestrial
20    isopod Porcellio scaber, known as woodlouse. Woodlice, about 16 mm  long, live in the upper layer of
21    soil and surface leaf litter.  They break down organic matter and contribute to soil health, and are
22    commonly used in ecological studies. In the Jemec et al. (2008) study, woodlice  ate dry leaves that had
23    been soaked in nano-TiO2 dispersions (sonicated or non-sonicated).  The sonication process decreased the
24    mean agglomerate size from 780-970 nm in a non-sonicated dispersion to 350-500 run. The activities of
25    catalase and glutathione-S-transferase (GST), two anti-oxidative stress enzymes in the digestive gland
26    (hepatopancrea) were measured. The activities of both enzymes were decreased at 0.5, 2000, and
27    3000 (ig/g of non-sonicated nano-TiO2, but not at middle concentrations (1, 10, 100, and 1000 (ig/g)  of
28    non-sonicated nano-TiO2 or at any concentration (1000, 2000, and 3000 (ig/g) of sonicated nano-TiO2
29    (Jemec et al., 2008)  No changes in feeding rate, defecation rate, food assimilation efficiency, weight, or
30    mortality were noted at concentrations up to 3000 (ig/g of either sonicated or non-sonicated nano-TiO2 in

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 1    the food. This study illustrates the importance of nano-TiO2 dispersion preparation method on nano-TiO2
 2    toxicity.
 3          Wang et al. (2009a) investigated the lethality, growth inhibition, and effects on reproduction of
 4    nano-TiO2 and conventional TiO2 in the nematode, C. elegans, a small free-living (i.e., not parasitic)
 5    roundworm that inhabits soil in temperate climates around the world and feeds on bacteria and fungi. In
 6    the laboratory, C.  elegans is often cultured on agar plates or in liquid medium in a Petri dish and is often
 7    fed E. coli. In the Wang et al. (2009a) study, C. elegans strain Bristol N2 (wild-type) in LI stage (larvae
 8    before the first molting) was exposed to anatase nano-TiO2 and anatase conventional TiO2 in water. In
 9    addition to lethality and growth inhibition, decreased reproduction was observed at lower mass
10    concentrations of nano-TiO2 than conventional TiO2. The tested reproduction parameters were eggs
11    inside body and the number of offspring per worm, which includes offspring at all stages beyond the egg
12    over the entire brood period. The mechanism of reproductive effects was not investigated. Due to the
13    lack of toxicity of supernatant of nano-TiO2 (obtained by centrifuging the nano-TiO2 suspension),
14    dissolution of the particle does not contribute to observed nano-TiO2 effects on C. elegans (Wang et al.,
15    2009a).

      5.2.1.4. Indirect and Interactive Ecological Effects
16          In addition to the direct toxicity of nano-TiO2, indirect effects of nano-TiO2 could also be
17    important.  Nano-TiO2 could adsorb pollutants (Nagaveni et al., 2004; Pena et al., 2006), carry the
18    pollutants into areas in an organism that the pollutants alone would not naturally appear (Moore, 2006),
19    and increase the uptake of other pollutants (a "Trojan horse" effect). Consequently, nano-TiO2 could
20    enhance pollutant toxicity, and even cause toxicities different from those caused by exposure to the
21    pollutant alone due to differences in distribution.  Also, as discussed in Section 4.2, co-exposure to nano-
22    TiO2 in water increased the uptake of arsenic (Sun et al., 2007)  and cadmium (Zhang et al., 2007) in carp,
23    but toxicity was not measured in these two studies.
24          Nano-TiO2 was found to have no effect on the toxicity of sediment and its elutriate in a study using
25    certified reference material sediment (Blaise et al., 2008). The effects of 11 nanomaterials on sediment
26    toxicity (as measured in two direct contact assays, the Microtox solid phase assay8  and the Luminotox
27    solid phase assay9) and sediment elutriate toxicity (as measured with the MARA assay10) were studied
       Microtox assay measures the reduction in light output from bioluminescent bacteria, Vibrio fischeri. For solid-
       phase assays, the concentration that causes 25% inhibition (IC25) is calculated after 20 minutes of exposure.
      9 Luminotox assay measures the inhibition of photo synthetic efficiency of photosynthetic enzyme complexes
       isolated from spinach leaves. For the Luminotox solid-phase assay, IC20 is calculated after 15 minutes of
       exposure.
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 1    using a mixture of each nanomaterial and the certified reference material sediment at a 1:1 ratio.
 2    Photocatalytic nano-TiO2 was one of only three tested nanomaterials that did not increase the sediment or
 3    elutriate toxicity in any of the three assays (Blaise et al., 2008).

      5.2.1.5. Summary
 4          Limited ecological toxicity information on nano-TiO2 is currently available.  Most ecotoxicological
 5    studies have tested photocatalytic nano-TiO2 that would be suitable for water treatment, but only a few
 6    studies have used photostable nano-TiO2 intended for sunscreen. Coated photostable nano-TiO2 in
 7    sunscreen could lose its coating through processes such as aging, weathering, chemical alterations (e.g.,
 8    change in pH), and metabolism or biotransformation in living organisms (e.g., digestion by daphnids).  If
 9    so, the photocatalytic nano-TiO2 core could be exposed and thus even photostable nano-TiO2 could have
10    photocatalytic propertie s.
11          Effects of chronic exposure to nano-TiO2 have been investigated only in water fleas and fish.
12    Although acute exposure effects have been studied in microorganisms and various aquatic
13    macroorganisms, these studies focused on lethality or immobility and provided little insight on modes of
14    action. For terrestrial organisms, only acute exposure to anatase nano-TiO2 was investigated and only in
15    invertebrates (P. scaber and C. elegans) and spinach. Photocatalytic nano-TiO2 decreased reproduction in
16    C. elegans without affecting body length. Although increased growth in spinach following acute
17    exposure to anatase nano-TiO2 could be useful for agricultural purposes, the effects of such growth
18    promotion in an ecological system remain unclear.  Photocatalytic nano-TiO2 enhanced the uptake of
19    arsenic and cadmium in fish, indicating the possibility of interactive effects between nano-TiO2 and co-
20    occurring toxic substances.
      10 MARA assay (microbial array for risk assessment assay) measures growth inhibition in baking yeast and ten
       species of bacteria.  A microbial toxic concentration is calculated after 18 hours of exposure.

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                      Questions  about  Ecological  Effects
 5.2-1.  Are current EPA standard testing protocols adequate to determine nano-Ti02 ecotoxicity? If not, what modifications
        or special considerations, if any, should be made in current ecological tests? For example, what are the differences
        in characterization of testing material (as raw material, in media, and in organisms), dispersion methods, and realistic
        exposure routes between testing conventional materials and nanomaterials?

 5.2-2.  What are the ecological effects of waste and other by-products of nano-Ti02 manufacturing?

 5.2-3.  Could ecological effects of pure nano-Ti02 be predictive of effects from products containing  nano-Ti02 (e.g.,
        containing stabilizers or surfactants)?

 5.2-4.  How can contributions of various nano-Ti02 physicochemical properties to nano-Ti02 ecological effects be identified
        or compared? For example, could a retrospective analysis of many studies and computer modeling identify patterns
        that would not be evident in individual studies? Is a structure activity relationship (SAR) approach applicable for
        predicting nano-Ti02 ecological effects?

 5.2-5.  What might be the primary mechanism(s) of action of toxic effects in different species?

 5.2-6.  Are the mechanisms of cellular responses different at low and high concentrations of nano-Ti02?

 5.2-7.  How do abiotic factors in the environment, such as UV, pH, oxygen level, and other chemicals, affect nano-Ti02 and
        its ecological effects?

 5.2-8.  How do in vivo biochemical processes alter nano-Ti02 physicochemical characteristics and toxicity?

 5.2-9.  What are the ecological effects of long-term exposure to nano-Ti02?

5.2-10.  What are the indirect ecological effects (e.g., on soil or water chemistry) of nano-Ti02?

5.2-11.  Nano-Ti02 has anti-bacterial and anti-fungal properties. What are the effects of both photocatalytic and photostable
        nano-Ti02 on the biodiversity of microorganisms?

5.2-12.  In addition to arsenic and cadmium, do other compounds show different uptake in the presence of nano-Ti02? Are
        the toxicities of arsenic,  cadmium, or other chemicals affected by nano-Ti02? Conversely, do other compounds
        affect the uptake and toxicity of nano-Ti02?

5.2-13.  Is the available ecotoxicity evidence adequate to support ecological risk assessment for nano-Ti02?  If not, what is
        needed?
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      5.3.  Health Effects
 1          This section summarizes and evaluates the evidence of nano-TiO2-induced health effects from
 2    epidemiological studies, laboratory animal studies, and a few selected ex vivo and in vitro studies. For a
 3    review of nano-TiO2 in vitro effects, see Fond and Meyer (2006).  Organized by human and laboratory
 4    animal studies and route of exposure, non-carcinogenic effects are discussed in Section 5.3.1;
 5    carcinogenic effects in Section 5.3.2.
      5.3.1. Non-Carcinogenic Effects
 6          This section summarizes in vivo studies of nano-TiO2 non-carcinogenic effects through dermal,
 7    oral, respiratory, and other routes of exposure. The presentation is organized by exposure routes, because
 8    exposure routes play a profound role in toxicokinetics, toxicodynamics, and health effects. More studies
 9    have been completed on respiratory exposure (inhalation and instillation) than on other exposure routes.
10    Studies investigating solely skin penetration (not health effects) are discussed in Section 4.6.2. Most
11    studies tested photocatalytic nano-TiO2, which could be suitable as an agent in drinking water treatment.
12    Commercial sunscreens were tested in dermal exposure studies only.  Known photostable nano-TiO2 and
13    rutile nano-TiO2, which is expected to be photostable, were used in some studies (Chen et al., 2006; Mohr
14    et al., 2006; Nemmar et al., 2008; Oberdorster et al., 1992; Pott and Roller, 2005; Wang et al., 2007a,
15    2007b; Warheit et al., 2007a, 2007b).

      5.3.1.1. Studies in Humans
16          No epidemiological studies or case reports are available for nano-TiO2 non-carcinogenic effects. A
17    few case reports described non-carcinogenic effects in the respiratory system of workers exposed to TiO2
18    particles of unspecified size. For example, exposure to conventional TiO2 has been associated with
19    pneumoconiosis (Yamadori et al., 1986), pulmonary fibrosis and bronchopneumonia (Moran et al., 1991),
20    and pulmonary alveolar proteinosis (Keller et al., 1995). TiO2 or titanium accumulation in the lung,
21    sometimes years after workplace exposures, and titanium-loaded macrophages have also been reported in
22    workers (Keller et al., 1995; Maatta and Arstila, 1975; Yamadori et al., 1986), as have titanium particles in
23    the lymph nodes (Maatta and Arstila, 1975; Moran et al., 1991) and in the liver and spleen (Moran et al.,
24    1991). None of these case reports, however, provided  quantitative TiO2 exposure data or measured
25    potentially confounding variables such as exposures to crystalline silica and tobacco smoke.
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 1          One epidemiological study (Chen and Fayerweather, 1988) found no consistent relationship
 2    between TiO2 (size not specified) exposure and chronic respiratory disease or fibrosis, but no conclusions
 3    can be drawn because of serious limitations, including restricting subjects to workers eligible for
 4    pensions; lack of information on the duration of TiO2 exposure, asbestos or other chemical exposures; and
 5    the lack of detailed information on sampling.

      5.3.1.2. Animal Studies
 6          For the most part (except as noted below), laboratory animal toxicity studies have investigated the
 7    effects of acute or subchronic exposure to nano-TiO2.  This section presents in vivo studies of nano-TiO2
 8    (Tables  5-4 to 5-7) by route of exposure: dermal, oral, respiratory, and others. Most animal studies of
 9    nano-TiO2 focus on photocatalytic nano-TiO2, including P25.  Although sunscreen nano-TiO2
10    formulations are intended to be photostable, the coatings that impart photostability to anatase or part-
11    anatase  nano-TiO2 in some sunscreen formulations are known to degrade over time (Barker and Branch,
12    2008; Dunford et al., 1997).

13    5.3.1.2.1. Toxicity from Dermal Exposure
14          Toxicity findings from studies of dermal exposure to nano-TiO2 or sunscreen that contains TiO2 are
15    presented in Table 5-4. For healthy unflexed skin, adverse health effects are not expected from dermal
16    exposure to photostable nano-TiO2 in sunscreen (NANODERM, 2007; SCCP, 2007). Photocatalytic
17    nano-TiO2, however, sometimes is used in sunscreens (Barker and Branch, 2008; Dunford et al., 1997).
18    Photocatalytic nano-TiO2 can generate ROS when exposed to UV and can cause oxidative stress and
19    cytotoxicity in cells (cultured human fibroblasts) and in cell-free in vitro experiments (Dunford et al.,
20    1997; Lu et al., 2008). To date, the effects of long-term or repeated use of sunscreen containing nano-
21    TiO2 have not been investigated in vivo, and no case reports of skin damage from such use are currently
22    available.  As discussed earlier, most available studies indicate penetration of the outer skin layer and the
23    stratum  corneum, but not penetration of living skin cells.
24          After a single topical application of photocatalytic nano-TiO2, laboratory animals showed no skin
25    irritation 4 hours after application or sensitization 3 days after application (Warheit et al., 2007a).
26    Furthermore, although some sunscreens containing TiO2 (size not specified) increased skin absorption of
27    herbicides and pesticides (2,4-D, paraquat, parathion or malathion), TiO2 alone actually decreased the
28    skin absorption of the tested herbicide, 2,4-D (Brand et al., 2003). The investigators reported that a
29    solvent  in the sunscreen caused increased  skin absorption of herbicides, and this secondary effect can be
30    avoided by substituting phenyl  trimethicone as the solvent (Brand et al., 2003).
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 1          Some researchers, such as Nohynek et al. (2007), have noted a discontinuity between in vitro and
 2    in vivo testing results, particularly for skin toxicity. Some in vitro cultures or preparations (other than
 3    those using intact skin samples) lack the stratum corneum layer, which according to currently available
 4    data can block penetration, such that in vitro tests might overstate toxicity of chemicals like TiO2.  Of the
 5    investigations reviewed, only three report in vivo  studies  of health effects after dermal exposure to TiO2
 6    [(Warheit et al., 2007a); pages 16, 17, 41-43 of (NANODERM, 2007)], and only two of those used nano-
 7    TiO2 intended for sunscreen [pages 16, 17, 41-43 of (NANODERM, 2007)]. [Warheit et al. (2007a) used
 8    ultrafine particles, roughly 100 nm in size.] All three studies used a single application, and the longest
 9    exposure was only 3 days. The NANODERM (2007) report concluded that  "TiO2 exposure did  not
10    modify the viability, proliferation, apoptosis, and differentiation [or] adhesive properties of skin cells." As
11    discussed previously, skin penetration studies have shown that some nano-TiO2 can stay in hair follicles
12    for 10 days.
13          With relatively few in vivo dermal exposure studies investigating nano-TiO2 skin absorption and
14    penetration (Table 4-5) and health effects (Table 5-4), several data gaps on the health effects of dermal
15    exposure to nano-TiO2 are evident. First, information on  the dermal penetration and effects of nano-TiO2
16    in flexed skin and structurally compromised skin is lacking. Flexed healthy  skin (Rouse et al., 2007;
17    Zhang and Monteiro-Riviere, 2008) and compromised skin (Zhang and Monteiro-Riviere, 2008),
18    including UV-exposed skin (Mortensen et al., 2008), have been shown to allow nanoparticles  (other than
19    nano-TiO2, which was not tested) to penetrate deeper than healthy non-flexed skin.  Sunscreen containing
20    nano-TiO2 is expected to be used on flexed healthy skin and misused on sunburned skin or skin with
21    micro-lesions, such as microscopic cuts due to shaving. Cytotoxicity was seen in cultured skin cells
22    treated with nano-TiO2 (Kiss et al., 2008), and the authors postulated that, in skin with compromised
23    epidermis structure  (e.g., sunburned skin or "soaked" skin), contact could occur between nano-TiO2 from
24    sunscreen and living cells in the skin and lead to adverse  effects. Second, effects from long-term,
25    repeated dermal exposures to nano-TiO2 in sunscreen, similar to real-life exposure, have not been studied.
26    Finally, the toxicity of the various intermediate forms of nano-TiO2 in the production process  (possible
27    sources of occupational exposure, by the dermal and other routes)  has not been  studied.
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Table 5-4.        Summary of health effects of nano-TiCh particles  in mammalian animal models: dermal route.3
   Animal
    Testing Material
              Treatment Conditions
                       Summary of Major Effects
     Reference
Mouse skin
[female hairless
(CRLSKH1)]
Commercially available
sunscreens, some of which
contained Ti02 (size not
specified)
For testing indirect dermal effect
a) Commercially available sunscreens, applied at 2 mg/cm2
to skin excised from mice and placed in a diffusion
chamber. 30 minutes after the sunscreen application,
herbicide 2,4-D was applied on skin.
b) Combination of Ti02with phenyl trimethicone, ZnO, and
octyl methoxycinnamate (OM)
c) TiSilc Untinted sunscreen, which contains Ti02, and
herbicide 2,4-D.  Both were applied on skin, and then again
4.5 hours after the first application
d) TiSilc Untinted sunscreen and pesticides: Paraquat,
Malathion,  and Parathione
Some (not all) tested sunscreens increased transdermal penetration of
herbicide/pesticide.
Solvent, not Ti02 or ZnO, is responsible for sunscreen-increased skin absorption of
herbicide/pesticide.
  a) Sunscreen effect on transdermal penetration of herbicide 2,4-D: 4 out of 7
  tested sunscreens that contain Ti02 (and 1 out of 2 sunscreens that contain no
  TiC)2) increased transdermal penetration of herbicide 2,4-D.
  b) Formulation effects: Ti02 alone, Ti02 plus ZnO, and Ti02 in trimethicone
  (simulation of commercial formula) decreased 2,4-D transdermal penetration.
  c) Repeated application of both sunscreen and herbicide: The peak penetration of
  2,4-D herbicide was higher at the second application of TiSilc sunscreen and  2,4-
  D, compared to the first application of TiSilc and 2,4-D. However, the 2,4-D
  penetrations of first and second applications of TiSilc and 2,4-D were the same
  when skin was washed after both (but not just one) applications of TiSilc and  2,4-
  D.
  d) Sunscreen effect on transdermal penetration of other pesticides: Absorption of
  other pesticides (Paraquat, Malathion, and Parathione) was also increased in skin
  pretreated with sunscreen Ti-Silc.
Brand etal. (2003)
Human foreskin
grafts on SCID
A commercially available
sunscreen, hydrophobic
emulsion containing nano-
Ti02(AntheliosXLSPF60,
La Roche Posay, France)
For testing dermal effects
Sunscreen containing nano-Ti02 applied to skin at 2
mg/cm2 in occlusion for 1, 24, or 48 hours
Sacrificed after exposure time; punch biopsy from the
human skin graft area
No effects on cell proliferation (as measured by bromo-deoxy-uridine, BrdU,
labeling); apoptosis (as measured by a double-staining method of Ki67 and TUNEL,
terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling); adhesive
properties (as measured by the expression of P-cadherin, an adhesion molecule
specific for basal epidermal keratinocytes); or differentiation (as measured by the
expressions of keratin-1, keratin-10, and filaggrin) of epidermal keratinocytes.
Tested sunscreen containing nano-Ti02did not affect viability, proliferation,
apoptosis, differentiation, or adhesive properties of skin cells.
Pages 16,17, and 41-
43ofNANODERM
(2007)
Rabbit
[New Zealand
White]
Nano-Ti02 (P25—identified
as uf-C in study),
photocatalytic, 80%
anatase/20% rutile, not
coated, average particle size
129.4 nm in water, average
BET surface area 53.0 m2/g
(Warheit, pers. comm.,
2008b)
For testing acute dermal irritation
Doses - 0 or 0.5 g
Single exposure for 4 hours (nano-Ti02 in 0.25 ml
deionized water on 6 cm2 area of skin), covered by gauze
Observation at  1,  24, 48, and 72 hours after exposure
No dermal irritation effects, no clinical signs of toxicity, and no body weight loss.
Not considered a skin irritant.
Warheit etal. (2007a)
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Table 5-4.  Summary of health effects of nano-TiCh particles in mammalian animal models: dermal route (continued).3
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Mouse
[female
CBA/JHsd]
Nano-Ti02 (P25),
photocatalytic, 80%
anatase/20% rutile, not
coated, average particle size
129.4 nm in water, average
BET surface area 53.0 m2/g
(Warheitpers. comm.,
2008b)
Diluting vehicle: N,N-
Dimethyl formamide
For testing dermal sensitization (local lymph node assay)
0, 5, 25, 50, or 100% nano-Ti02 on both ears for 3 days
Positive control group: 25% hexylcinnamaldehyde in 4:1
acetone:olive oil for 3 days
(Vehicle of positive control) group: 4:1 acetone:olive oil for
3 days
Sacrifice on test day 5
Increases in cell proliferation in the draining auricular lymph node of the ears treated
with 50% and 100% nano-TiCfe compared to the vehicle control group.
No dermal sensitization by nano-TiCfe Stimulation index [mean disintegrations per
minute of each experimental group / mean disintegrations per minute of the vehicle
control group] did not exceed 3.0 in any nano-Ti02 treated groups.  Consequently
the EC3 value (the estimated concentration required to induce a threshold positive
response, i.e., where stimulation index equals 3) for nano-Ti02 was not calculated.
Positive control group had a dermal sensitization response.
Warheitetal. (2007a)
  BET - Brunauer, Emmett, Teller method of calculating surface area
  BrdU - Bromo-deoxy-uridine
  EC3 - Estimated concentration required to induce a threshold positive response, where stimulation index
  equals 3
                                                                            OM -Octyl methoxycinnamate
                                                                            TUNEL -Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling
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 1    5.3.1.2.2. Toxicity from Oral Exposure
 2          Currently only three toxicological studies of nano-TiO2 through oral exposure are available (Table
 3    5-5). Two of them observed the toxicity for up to 2 weeks after a single oral gavage of nano-TiO2 (Wang
 4    et al., 2007a; Warheit et al, 2007a), and the other investigated genomic instability after nano-TiO2
 5    exposure through drinking water for 5 or 10 days (Trouiller et al., 2008).
 6          The Warheit et al. study (2007a) was intended to provide basic hazard screening information on
 7    well-characterized types of nano-TiO2 through a "base set" of tests spanning mammalian toxicity,
 8    genotoxicity, and aquatic (ecological) toxicity endpoints. The acute oral toxicity aspect of this project
 9    involved female rats receiving a single oral gavage of up to 5000-mg/kg photocatalytic nano-TiO2 (P25)
10    (3 rats per dose). The authors reported "no biologically important body weight loss" and no gross lesions
11    at necropsy 14 days after the gavage. Given that this was a basic screening study, no information on
12    organ weights, histological examinations, or blood tests (hematological or biochemical) was obtained, and
13    thus it was not meant to rule out systemic toxicity or functional changes. However, the study does
14    provide evidence that up to 5000-mg/kg nano-TiO2 was not lethal as tested.
15          In the Wang et al. study (2007a), male and female mice received a single oral gavage of 5000-
16    mg/kg TiO2 as 25-nm rutile spindles, 80-nm rutile spindles, or 155-nm anatase octahedrons (see Table 5-5
17    for more details). The large dose was selected because of the expected low toxicity and was
18    administrated according to OECD testing procedures. No obvious acute toxicity was evident over a
19    2-week period. However, liver and kidney toxicity were indicated by biochemical parameters in the
20    serum and by pathological examination. Although no abnormal pathology was observed in the  heart,
21    lung, testicle/ovary, and spleen tissues, myocardial damage was suggested by increases in serum lactate
22    dehydrogenase (LDH) and alpha-hydroxybutyrate dehydrogenase (a-HBDH), although such increases
23    might also reflect damage to other organs.  Morphological changes in the brain were seen in the
24    hippocampus in both the 80-nm and 155-nm groups. The main organs with elevated TiO2 concentrations
25    (measured only in female mice) were the liver, spleen, kidneys, lungs, and brain. Although the  liver is
26    expected to receive most of the TiO2 absorbed from the gastrointestinal tract through the portal  vein,
27    elevated TiO2 levels in the  liver were observed only in the 80-nm group.  The reason for this size-specific
28    elevation in hepatic TiO2 concentration remains unknown.
29          The preliminary results of the Trouiller et al. (2008) study showed increased DNA and
30    chromosomal damage in various tissues of mice given 60-600 (ig/mL photocatalytic nano-TiO2 (P25) in
31    drinking water for 5 days.  Furthermore, the offspring of mice that were given nano-TiO2 in drinking
32    water in the second half of the pregnancy showed increases in DNA deletions in the eye-spot assay
33    (Trouiller et al., 2008), which detects reversion of the mouse pink-eyed unstable (pun) mutation through

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 1    DNA deletions of duplicated pink-eyed dilution (p) gene in the offspring of C57Bl/6J//m///m mice (Reliene
 2    and Schiestl, 2003; Schiestl et al., 1997). This study showed not only genotoxicity and clastogenicity, but
 3    also multi-generation effects of photocatalytic nano-TiO2 through oral exposure. Although the
 4    concentrations investigated in this study are very high, the suggested modes of action and effects of
 5    exposure during pregnancy are noteworthy, particularly for photocatalytic nano-TiO2. This work is also
 6    relevant to discussions of the carcinogenicity of nano-TiO2 (see Section 5.3.2). The application of
 7    genotoxicity data to the question of potential carcinogenicity is based on the premise that genetic
 8    alterations are found in all cancers. Mutagenicity/genotoxicity is the ability of chemicals to alter the
 9    genetic material in a manner that permits changes to be transmitted during cell division. Although most
10    tests for mutagenicity  detect changes in DNA or chromosomes, some specific modifications of the
11    epigenome including proteins associated with DNA or RNA, can also cause transmissible changes.
12    Genetic alterations can occur via a variety of mechanisms including gene mutations, deletions,
13    translocations, or amplification; evidence of mutagenesis provides mechanistic support for the inference
14    of potential for carcinogenicity in humans.
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Table 5-5.        Summary of health effects of nano-TiCh particles in mammalian animal models: oral route.3
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Mouse
[wild-type and
C57BI/6J/P//P]
Nano-Ti02 (P25),
photocatalytic, 80%
anatase/20% rutile, not
coated
For testing genotoxicity in two generations
Wild-type mice: 60,120, 300 and 600 pg/mL in drinking
water for 5 days (Based the assumption of 5 ml water
intake per day per mouse with a BW of 30 g, the total doses
would be 50,100, 250 and 500 mg/kg body weight)
C57BI/6Jpun/pun mice for eye-spot assay: 10-day
exposure, pregnant mice were given nano-Ti02 in drinking
water from 8.5 to 18.5 days post conception. Offspring
were sacrificed at 20 days old.
Increased genomic instability in exposed mice:
  DMA damage was increased in cells in peripheral blood at 600 ng/ml_. DMA
  damage was measured by alkaline Comet assay, which detects DMA single strand
  breaks, double strand breaks, alkaline liable sites, and other lesions.
  DMA double strand breaks (measured by yH2AX immuno-staining) were
  increased in bone marrow at all tested doses.
  Chromosomal damage (measured by micronucleus assay) was increased in
  peripheral blood at 600 ng/ml_.
  Oxidative DMA damage (measured by HPLC) was increased in liver at 600
  ng/mL
Increased genomic instability in the offspring of dams exposed to nano-Ti02 during
pregnancy:
  Increases in DMA deletions at the pink-eyed unstable (pun)  locus [from
  homologous recombination or double strand breaks between the DMA fragments
  that contain duplicated pink-eyed dilution (p) gene (Reliene  et al., 2003)]  as
  measured by  the eye-spot assay at 500  mg/kg.
Increased inflammation:
  Increases in (mRNA levels of) pro-inflammation markers,  TNF-a, IFN-y, and IL-8
  (KG) (but not anti- inflammatory markers, TGF-p, IL-10 or IL-4) in peripheral blood
  at 500 mg/kg as measured by real time RT-PCR.
Trouilleretal. (2008)
Rat
[female,
strain/stock not
specified]
Nano-Ti02 (P25) (identified
as uf-c), photocatalytic, 80%
anatase/20% rutile, not
coated, average particle size
129.4 nm  in water, average
BET surface area 53.0 m2/g
(Warheit, pers. comm.,
2008b)
For testing acute effects
Doses - 175, 550,1750, or 5000 mg/kg (three rats per
dose)
Single oral gavage
Observation for 14 days post exposure
No mortality, no biologically important body weight losses, and no gross lesions
present in the rats at necropsy.
Grey colored feces was observed in rats dosed at 1750 mg/kg (one rat) and 5000
mg/kg (three rats).
Oral LDso>5000 mg/kg for female rats.
Warheit etal. (2007a)
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Table 5-5. Summary of health effects of nano-TiCh particles  in mammalian animal models: oral route (continued).3
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Mouse
[male and
female CD-1
(ICR)]
Nano-Ti02 (Hangzhou
Dayang Nanotechnology Co.
Ltd.), rutile, uncoated, 25 nm
(measured average size
21.1±5.1 nm), surface area
43.0 m2/g, column/spindle
shape, purity >99% (Chen,
2008)
Nano-Ti02 (Hangzhou
Dayang Nanotechnology Co.
Ltd.), rutile, uncoated, 80 nm
(measured average size
71.4±23.5 nm), surface area
22.7 m2/g, column/spindle
shape, purity >99% (Chen,
2008)
Fine Ti02 (Zhonglian
Chemical Medicine Co.),
155 nm (measured average
size155.0±33.0nm),
surface area 10.4m2/g,
anatase, uncoated,
octahedrons, purity >99%
(Chen, 2008)
Single oral gavage (acute effects)
Dose - 5000 mg/kg
10 female and 10 male mice per Ti02 size group
Necropsy at 2 weeks after the gavage
Hepatic toxicity:
  Increases in coefficients (wet organ weight/body weight) of liver (females in 25 nm
  and 80 nm groups), serum ALT (females in 25 nm group), serum ALT/AST
  (females in 25 nm group and males in 155 nm groups), and serum LDH (females
  in 25 nm and 80 nm groups).b Decreases  in AST in males in the 155 nm group
  (Chen, 2008).
  Pathological changes: hydropic degeneration around the central vein, spotty
  necrosis of hepatocytes (males and females in 80 nm and 155 nm groups).
Nephrotoxicity:
  Increases in serum BUN (females in 25  nm group; no tin  males) and serum LDH
  (females in 25 nm and 80 nm groups; male data not available) (Chen, 2008).b
  Pathological changes: swelling in renal glomerules and proteinic liquid in renal
  tubule (males and females in 80 nm group).
Possible brain toxicity:
  Pathological changes: increases in vacuoles in the neuron of the hippocampus
  (males and females in 80 nm and  155 nm groups). The vacuoles could be from
  reversible fatty degradation (Chen, pers. comm., 2008).
Possible myocardial damage:
  Increase in serum LDHb (females in 25  nm and 80 nm groups; male data not
  available), a-HBDH (females in 25 nm and 80 nm groups; male data not available)
  (Chen, pers. comm., 2008). Based on the data in this study alone, it cannot be
  ruled out that LDH and a-HBDH were from kidney or liver.
  No pathological changes in heart.
No pathological changes in heart, lung, testicle/ovary or spleen in male and female
mice exposed to either 80 nm or 155 nm TiCt No pathological changes in any
organs of mice exposed to 25 nm Ti02.
Ti02 distribution in female mice: increased Ti concentrations in liver (80 nm group),
spleen (25, 80,155 nm groups), kidney (25, 80 nm groups), lung (80 nm group) and
brain (25, 80, 155 nm groups). For the 80  nm group, highest Ti concentration was in
liver (3970 ng/g), followed by spleen, kidney, and lung (-375-625 ng/g). For 25 nm
group, highest Ti concentration was in spleen (-500 ng/g).
Wangetal.(2007a)
Chen (2008)
  a-HBDH - Alpha-hydroxybutyrate dehydrogenase
  yH2AX - Phosphorylated form of histone H2AX (phosphorylation of H2AX at serine 139)
  ALT-Alanineaminotransferase
  AST-Aspartate aminotransferase
  BET - Brunauer, Emmett, Teller method of calculating surface area
  BUN - Blood urea nitrogen
  HPLC - High performance liquid chromotography
  IFN-y- Interferon-gamma
                                                                               IL-4-lnterleukin-4
                                                                               IL-8 (KG) - IL-8 stands for interleukin-8 and KG for chemokine (CXC motif) ligand 1 (CXCL1)
                                                                               IL-10-lnterleukin-10
                                                                               LDH - Lactate dehydrogenase, a general marker of cell injury (Ma-Hock et al., 2009)
                                                                               LDso- Lethal dose 50; the dosage that is lethal to 50% of the tested population
                                                                               RT-PCR - Reverse transcription polymerase chain reaction
                                                                               TGF-p - Transforming growth factor-beta
                                                                               TNF-a - Tumor necrosis factor-alpha
  LDH may be from heart, liver, kidney, skeletal muscle, brain, blood cells, and lungs.  A test for LDH isotypes can help to narrow down the source.  The primary sources for various LDH isotypes in humans are:
  LDH-1 from heart muscle and red blood cells; LDH-2 from white blood cells; LDH-3from lung; LDH-4from kidney, placenta, and pancreas; and LDH-5from liver and skeletal muscle (Abraham etal., 2009).
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 1    5.3.1.2.3. Toxicity from Respiratory Exposure
 2          This section discusses the health effects of nano-TiO2 exposure through the respiratory tract (Table
 3    5-6).  Two methods of exposure commonly employed for studies of respiratory toxicity are inhalation and
 4    instillation.  Instillation can be performed in various ways, but essentially involves the direct
 5    administration of a substance to the lungs rather than allowing the subject to inhale the material.
 6    Intratracheal instillation "can be a useful and cost-effective procedure for addressing specific questions
 7    regarding the respiratory toxicity of chemicals, as long as certain caveats are clearly understood and
 8    certain guide lines are carefully followed" (Driscoll et al, 2000). Among the advantages of instillation are
 9    that it permits researchers to control the doses administered into the lung and allows fast administration of
10    test material to the lower respiratory tract. Instillation studies can be useful for identifying most types of
11    effects (other than upper respiratory tract effects, such as nasal effects) and for comparing the relative
12    potency of compounds, and for this reason are of interest for screening different materials for toxicity.
13    Additionally, instillation studies require smaller amounts of test material, and chances of incidental
14    ingestion exposure (as in whole-body chamber inhalation) are lower than in inhalation studies (Driscoll et
15    al., 2000; Osier et al., 1997).  On the other hand, instillation exposure involves invasive  delivery,
16    bypassing of the upper respiratory tract, confounding effects  from the instilled vehicle, and the use of
17    higher doses or dose rates than those tested in inhalation experiments.  Confounding effects are also a
18    concern from anesthesia (needed for instillation, but not inhalation), which could affect the retention and
19    clearance of the test material  (Driscoll et al., 2000). Furthermore, studies have shown that exposure to the
20    same particle through intra-tracheal instillation and inhalation can yield different responses. For example,
21    compared to inhalation, instillation caused more particles to be deposited in the basal regions of the lung
22    and caused particles to be distributed less homogenously (Osier et al., 1997).  Also, results from
23    instillation cannot be extrapolated quantitatively for estimating inhalation results (Driscoll et al., 2000).
24          Interpreting and comparing results from studies with different respiratory exposure  methods (such
25    as inhalation, instillation, and aspiration) requires caution. Differences among exposure methods could
26    influence uptake doses and particle  distributions in the body. Also, the test material preparation required
27    for different exposure methods (such as aerosol and suspension medium preparation) could affect
28    nanomaterial aggregation.  Conclusions drawn from studies using  different methods should disclose
29    confounding factors to avoid  misleading readers. As an illustration, consider a study that exposed mice to
30    single-walled carbon nanotubes (SWCNT) through inhalation and pharyngeal aspiration (Shvedova et al.,
31    2008). Even though the doses were designed to generate the same deposited dose in the lung, the aerosol
32    generation and agglomerate sizes of the test material differed. The authors carefully stated their
33    conclusion at the end of discussion as:  "Because of exposure to smaller SWCNT structures by inhalation

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 1    of a dry aerosol vs. aspiration of a particle suspension containing micrometer-size agglomerates,
 2    inhalation exposure was more potent than aspiration of an equivalent mass of SWCNT."
 3          The tendency of nano-TiO2 to agglomerate raises an important issue for interpreting experimental
 4    toxicology studies when the respiratory tract is the portal of entry. Upon inhalation, insoluble particles
 5    will deposit in the lung according to the aerodynamic diameter of the particulate unit (i.e., the
 6    agglomerate) and the physiological/morphometric characteristics of the subject. Once deposited as a
 7    result of inhalation or intratracheal instillation, additional factors (e.g., physicochemistry of the particles,
 8    biochemistry of the fluid lining of the lung, and other pharmacokinetic factors of the subject) may impact
 9    particle size and composition and determine the ultimate dose to the target cell/molecule.  The influence
10    of the lung milieu on agglomeration is discussed in more detail below.
11          It should be noted that the concentrations in available respiratory toxicity studies of nano-TiO2 are
12    presumably much higher than likely ambient or occupational exposure levels. High concentrations of
13    fine-mode particles are known to cause the phenomenon of "particle overload." In  its simplest terms, at
14    sufficiently high concentrations, the body's ability to clear inhaled particles is severely compromised to
15    the point that effects occur that would not occur at high-end "real-world" exposures [see ILSI Risk
16    Science Institute Workshop Participants (2000) for summary].  Thus, under particle overload conditions,
17    exposure-response relationships and even the type of responses produced can be unreliable.  However, the
18    nanoparticle exposures evoking particle overload have not been fully described.
      Effects in Respiratory Tract
19          As discussed below and summarized in Table 5-6, pulmonary effects studied through inhalation or
20    instillation of nano-TiO2 include pulmonary inflammation, recruitment of neutrophils and macrophages,
21    nano-TiO2 aggregate-loaded macrophages, disruption of alveolar spaces, alveoli enlargement,
22    proliferation of alveolar type II pneumocytes, and increases in alveolar epithelial thickness.  Selected
23    instillation studies are highlighted here primarily for effects not investigated in inhalation studies (i.e.,
24    effects outside the respiratory tract and interactions with other factors).
25          Some of the factors that affect nano-TiO2 respiratory tract toxicity were investigated by
26    Oberdorster et al. (2000). Toxicity of nano-TiO2 could be decreased by cross-tolerance to oxidative
27    stress, because nano-TiO2 given through an intra-tracheal instillation caused less inflammation in rats
28    previously exposed (and adapted) to Teflon fumes than in rats that were not adapted. Furthermore, nano-
29    TiO2 induced more severe pulmonary inflammation in compromised rats, which had been given an
30    endotoxin to mimic gram-negative bacterial infections, than in healthy rats.
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            Inhalation and Instillation in the Same Study
 1          Grassian et al. (2007a) exposed mice to nano-TiO2 through either inhalation or intranasal
 2    instillation. After instillation exposures to similar surface area doses (based on primary particle surface
 3    areas) of 5-nm anatase nano-TiO2 and 21-nm anatase/rutile nano-TiO2, mice showed a more severe
 4    inflammation response to 21-nm nano-TiO2 than to 5-nm TiO2. This example shows that surface area
 5    alone is not a sufficient dose metric in all studies (Grassian et al., 2007a; Warheit et al., 2007a), especially
 6    when the crystal form and other factors are not the same. In the Grassian et al. (2007a) study, the
 7    aggregates of 21-nm and 5-nm nano-TiO2 differed in both size and density, either of which could affect
 8    the surface area that would interact with the tissues. Although the same nano-TiO2 was used in both
 9    inhalation and intranasal instillation, direct comparisons of exposure routes effects were not feasible for
10    two reasons. First, the exposure doses were not the same, whether the doses were expressed as particle
11    concentrations in air or solution, estimated  particle mass per mouse, or estimated particle surface area per
12    mouse.  Second, different vehicles (water for inhalation and saline for instillation) were used and the sizes
13    of agglomerates were larger in inhalation aerosols than in instillation.
14          In a study by Osier et al. (1997), acute intra-tracheal inhalation of high levels (125 mg/m3) of fine
15    and nano-TiO2 caused less severe pulmonary response than intra-tracheal instillation.  Intra-tracheal
16    inhalation involved delivering aerosols to the trachea of anesthetized rats.
            Inhalation Studies
17          The effects in the respiratory tract after inhalation of nano-TiO2 were consistent among studies.
18    With increases in exposure duration, pulmonary lesions in rodents evolve from reversible pulmonary
19    inflammation (in rats, mice, and hamsters) to impaired particle clearance or overload (in rats and mice,
20    but not hamsters) and cellular proliferation (in rats and mice, but  not hamsters).  In rats, but not in mice or
21    hamsters, chronic exposure leads to pulmonary alveolar fibrosis, metaplasia, and eventually lung tumors.
22          In acute and subacute studies in mice and rats, the severity of pulmonary inflammation increased
23    with increases in exposure time, and symptoms (pulmonary inflammation and increases in cell
24    proliferation in  bronchi and bronchioles) were reversible when exposure ended (Grassian et al., 2007b;
25    Ma-Hock et al., 2009).
26          In subchronic studies of nano-TiO2 exposure for 12 or 13 weeks, pulmonary inflammation,
27    pathological changes in the lung (including fibrosis), and impairment of alveolar macrophage-mediated
28    test particle clearance were reported (Baggs et al., 1997; Bermudez et al., 2002; Bermudez et al., 2004;
29    Hext et al., 2002; Hext et al., 2005; Oberdorster et al., 1994).  Similar to pulmonary lesions after acute
30    and subacute exposure, pulmonary lesions after subchronic inhalation exposure were also decreased with
31    recovery time, but some lesions, such as fibrotic reactions in the lung, were not completely reversed even
32    after 1 year of recovery.

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 1          Species differences to nano-TiO2 effects were observed among rats, mice, and hamsters (Baggs et
 2    al., 1997; Bermudez et al., 2002; Bermudez et al., 2004; Hext et al., 2002; Hext et al., 2005; Oberdorster
 3    et al., 1994).  Pulmonary responses after 13 weeks of exposure were generally most severe in rats,
 4    followed by mice, and least severe in hamsters. Rats and mice, but not hamsters, experienced overload at
 5    10 mg/m2 nano-TiO2.  Furthermore, only rats had fibroproliferative lesions and alveolar epithelial
 6    bronchiolization (a type of metaplasia).
 7          In chronic studies of nano-TiO2 inhalation in rats (Creutzenberg et al., 1990; Gallagher et al., 1994;
 8    Heinrich et al., 1995) and mice (Heinrich et al., 1995), lung tumors occurred in rats, but not in mice (for
 9    more on carcinogenicity effects in these studies, see Section 5.3.2) . In the study of Creutzenberg et al.
10    (1990),  decreased pulmonary clearance (overload) was clearly demonstrated by using two sizes of tracer
11    particles after nano-TiO2 exposure.  During the 24-month exposure to nano-TiO2 (see Table 5-6 for
12    concentrations), rats inhaled (nose-only) two types of radioactive tracers at 3, 12, and 18 months after the
13    beginning of the experiment. The half-times for pulmonary clearance of the smaller tracer particles
14    (0.35-(im 59Fe2O3) were more than 3-times longer in rats exposed to nano-TiO2 at all three tested time
15    points, indicating overload. For the larger tracer particles (3.5-(im 85Sr polystyrene), overload was seen at
16    3 and 12 months, and the clearance was back to control level at 18 months, which may be due to
17    increased lung weight, altered lung structure, and altered breathing pattern, all of which could
18    consequently change the deposition of 85Sr polystyrene particles (Creutzenberg et al., 1990).
      Systemic Effects and Effects in Heart, Liver, Kidney, and Microvasculature
19          The effects of respiratory exposure to nano-TiO2 are not limited to the respiratory system. In rats
20    exposed to 5-mg nano-TiO2/kg BW of rutile nano-TiO2 rods through a single intra-tracheal instillation,
21    observed effects included increases in the numbers of monocytes and granulocytes in the blood (signs of
22    systemic inflammation); decreases in the number of platelets in the blood (platelet aggregation); and
23    cardiac  edema (Nemmar et al., 2008).  In mice exposed to rutile and anatase nano-TiO2 through intranasal
24    instillation, pathological changes were observed in the kidney, and temporary liver injury was suggested
25    by changes in serum biomarkers (Wang et al., 2008b).
26          Endothelium-dependent arteriolar dilation was impaired (decreased) by both fine TiO2 and nano-
27    TiO2 inhaled by rats, more so by nano-TiO2 than fine TiO2 at similar lung load mass doses (Nurkiewicz et
28    al., 2008). This microvascular dysfunction was due to fine TiO2- and nano-TiO2-induced increases in
29    ROS in the microvascular wall, increases in nitrotyrosine expression in spinotrapezius microcirculation,
30    and decreases in microvascular NO production (Nurkiewicz et al.,  2009).  In both fine TiO2-and nano-
31    TiO2-treated groups, vascular smooth muscle sensitivity to NO was not altered, but the microvascular NO
32    bioavailability was compromised (Nurkiewicz et al., 2009).
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      Effects in Brain
 1          Since 1970, scientists have known that inhaled ultrafine air pollutants and engineered nanoparticles
 2    translocate into the brain (Oberdorster et al, 2004).  Inflammatory responses, altered neurotransmitter
 3    levels, and pathological changes have been observed in rodent brains after inhalation of manganese oxide
 4    (Elder et al., 2006); instillation of nano carbon black (Tin Tin Win et al., 2008); and inhalation of ultrafine
 5    elemental 13C particles (Oberdorster et al., 2004). A few recent studies showed that anatase and rutile
 6    nano-TiO2 translocate into the brain following intranasal instillations (Wang et al., 2008a; Wang et al.,
 7    2008b, 2007b).
 8          The only available studies of nano-TiO2 effects on the central nervous system are from a research
 9    group that has administered nano-TiO2 to mice using intranasal instillation (Wang et al., 2008a; Wang et
10    al., 2008b; Wang et al., 2007b). These researchers have reported increased oxidative stress and
11    inflammatory response, altered concentrations and metabolism of neurotransmitters, and pathological
12    changes in the mouse brain. When mice were given 25-nm rutile, 80-nm rutile, or 155-nm anatase nano-
13    TiO2 though intranasal instillation (50 mg nano-TiO2/kg BW every two days for 2, 10, 20, or 30 days),
14    changes in neurotransmitter levels in the brain were observed only in mice exposed to 80-nm and 155-nm
15    nano-TiO2, whereas brain TiO2 concentrations were similar for all three sizes of nano-TiO2 (Wang et al.,
16    2007b). After intranasal instillation of 80-nm rutile or 155-nm anatase nano-TiO2 (500 (ig per mouse
17    every other day for up to 30 days), the highest titanium concentrations in the brain were in the
18    hippocampus and olfactory bulb, the two regions where most pathological changes were also seen (Wang
19    et al., 2008a; Wang et al., 2008b).  The hippocampus and astrocytes seem to be the targets of nano-TiO2
20    toxicity in the brain (Wang et al., 2008a; Wang et al., 2008b). At the ultra-structural level, mitochondria
21    appear to be a target of nano-TiO2 in nerve  cells after both in vivo and in vitro exposures (Long et al.,
22    2006; Wang et al., 2008b). For the whole brain, inflammatory responses and oxidative stress, including
23    lipid peroxidation and protein oxidation, were detected as elevated levels of oxidative  markers and
24    cytokines in mice exposed to 80-nm rutile and 155-nm anatase nano-TiO2 (Wang et al., 2008a; Wang et
25    al., 2008b).
26          Levels of several neurotransmitters, including norepinephrine, 5-hydroxytryptamine, homovanillic
27    acid, 5-hydroxyindole acetic acid, dopamine, and glutamic acid, were altered after intranasal instillation
28    of nano-TiO2 (Wang et al., 2008a; Wang et  al., 2008b; Wang  et al., 2007b).  Nitric oxide, which serves as
29    a neurotransmitter and an important player  in inflammatory responses, was also increased in the brain of
30    mice exposed to 80-nm and 155-nm nano-TiO2 (Wang et al.,  2008a). Additionally, the activity of
31    cholinesterase, which inactivates the neurotransmitter acetylcholine, increased (Wang  et al., 2008a).
32    These changes showed that the concentrations and metabolism of neurotransmitters in the brain were
33    affected by nano-TiO2 given through intranasal instillations.

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Table 5-6.        Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route.a
Animal
Testing Material
Treatment Conditions
Summary of Major Effects
Reference
Inhalation and Instillation in the same report
Mouse
[male C57BI/6]
Nano-Ti02 (Nanostructured
and Amorphous Materials),
anatase, 5 nm, measured
BET surface area
219±3m2/g, surface
functionalization: 0, 0-H,
H20. Aerosol size: 119±1.56
nm (inhalation high dose),
122.9±1.55 nm (inhalation
low dose)
Nano-Ti02 (Degussa),
anatase/rutile, 21 nm, BET
surface area 41±1.1  m2/g,
surface functionalization: 0,
0-H, H20. Aerosol size:
138.8±1.44 m2/g (inhalation
high dose), 152.9±1.38m2/g
(inhalation low dose)
Single inhalation exposure for 4 hours
Particle concentration in chamber:
  5 nm TiCt
    Low: 0.77 mg/m3 (necropsy immediately after
    exposure)
    High: 7.22 mg/m3 (necropsy immediately after
    exposure); 7.35 mg/m3 (necropsy 20 hours after the
    end of exposure)
  21 nm Ti02:
    Low: 0.62 mg/m3 (necropsy immediately after
    exposure)
    High: 7.16 mg/m3 (necropsy immediately after
    exposure); 7.03 mg/m3 (necropsy 20 hours after the
    end of exposure)
                                         Single intra-nasal instillation
                                         Particle concentration in instillation solutions:
                                           5 nm TiCt
                                             Low: 0.1 mg/mL
                                             Medium: 0.4 mg/mL
                                             High: 0.6 mg/mL
                                           21 nm Ti02:
                                             Low: 0.5 mg/mL
                                             Medium: 2.0 mg/mL
                                             High: 3.0 mg/mL
                                         Necropsy 24 hours after instillation
Increases in the numbers of total cell (high 5 nm, low and high 21 nm) and
macrophage (high 5 nm and 21 nm) in BAL fluid immediately after exposure (not 20
hours after exposure).
No changes in histology of the lung, total protein, LDH activity, or neutrophil number
in BAL fluid.
Nano-Ti02 distribution (only 4 high groups examined): agglomerates were seen in
macrophages, alveolar epithelial cells, and alveolar interstitium. Little difference
between 5 and 21 nm exposures or necropsy time.
Calculated/estimated particle mass per mouse (|jg) and particle surface area (cm2):
  5 nm Ti02 Low:  1.3 pg/mouse and 3.2  cm2 (immediately after exposure)
  5 nm Ti02 High:  12.5 pg/mouse and 30.3 cm2 (immediately after exposure)
                 12.7 pg/mouse and 30.7 cm2 (20 hours after exposure)
  21 nm Ti02 Low: 1.1 pg/mouse and 2.2  cm2 (immediately after exposure)
  21 nmTi02High: 12.4 pg/mouse and 24.8 cm2 (immediately after exposure)
                 12.2 pg/mouse and 24.4 cm2 (20 hours after exposure)
                                                                             21 nm Ti02 induced more inflammation than 5 nm TiCfe Increases in neutrophil
                                                                             number (21 nm low, medium and high; 5 nm medium and high); total cell number
                                                                             and IL-6 (21 nm medium and high); LDH activity and IL-1 (3 (21 nm high) in BAL fluid.
                                                                             No pathological changes in lung; no changes in TNF-a in  BAL fluid.
                                                                             21 nm anatase/rutile Ti02 and 5 nm anatase Ti02 do not share the same dose-
                                                                             response curve for neutrophil concentration in BAL fluid as a function to either
                                                                             particle mass or surface area.
                                                                             Calculated/estimated particle mass per mouse (|jg) and particle surface area (cm2):
                                                                                5nmTi02Low:      5 pg/mouse and 12.1 cm2
                                                                                5 nm Ti02 Medium:  20 pg/mouse and 48.4 cm2
                                                                                5 nm Ti02 High:      30 pg/mouse and 72.6 cm2
                                                                                21 nm Ti02 Low:     25 pg/mouse and 12.5 cm2
                                                                                21 nm Ti02 Medium:  100 pg/mouse and 50 cm2
                                                                                21 nm Ti02 High:    150 pg/mouse and 75 cm2
Grassian etal.
(2007a)
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Table 5-6. Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
Animal
Rats
[female F344]
Testing Material
Fine Ti02 (Fisher Scientific),
mean primary particle size
250 nm, anatase
Nano-Ti02 (Degussa), mean
primary particle size 21 nm,
anatase
Treatment Conditions
Acute intra-tracheal instillation and intra-tracheal inhalation
Intra-tracheal inhalation exposure for 2 hr at 125 mg/m3
Intra-tracheal instillation exposure to the equivalent amount
of Ti02 as in the lung at day 0 of intra-tracheal inhalation
(500 |jg fine Ti02 or 750 pg nano-Ti02 in 0.2 ml saline)
Necropsy 0, 1 , 3 or 7 days post exposure (three rats per
group)
Summary of Major Effects
Compared to fine Ti02, nano-Ti02 caused more pulmonary responses and slightly
higher (not significant) lung TiCh burden.
Compared to intra-tracheal instillation, intra-tracheal inhalation to Ti02 generally
caused less severe and less persistent pulmonary responses and slightly (not
significant) higher Ti02 lung burden.
Increases in polymorphonuclear leukocytes in BAL cell pellet on day 1 after intra-
tracheal inhalation of fine Ti02; on days 1, 3, and 7 after intra-tracheal instillation
of nano-Ti02; and days 0 and 1 after intra-tracheal inhalation of nano-TiCt
Decreases in macrophage inflammatory protein-2 levels in BAL supernatant on
days 0,1, and 3 after intra-tracheal inhalation of nano-Ti02; and day 1 after intra-
tracheal instillation of nano-TiCt Increases in macrophage inflammatory protein-2
levels in BAL cell pellets on days 1, 3, and 7 after intra-tracheal instillation of
nano-Ti02; and on days 0 and 1 after intra-tracheal inhalation of nano-TiCt
Increases in TNF-a protein was detected by immunocytochemistry (but not by
ELISA) on days 0 and 1 after intra-tracheal inhalation of water (control); days 1
and/or 3 after intra-tracheal instillation of fine or nano-Ti02 and intra-tracheal
inhalation of fine Ti02; and at all time points after intra-tracheal inhalation of nano-
Ti02.
Inflammatory cell influx (polymorphonuclear leukocytes in BAL) was correlated
with macrophage inflammatory protein-2 levels in BAL cell pellet (but not in BAL
supernatant), but not correlated with TNF-a protein levels in BAL cell pellet or
supernatant or in lung sections stained immunocytochemically.
Reference
Osier etal. (1997)
Inhalation
Rats
[male F344]
Nano-Ti02, -20 nm, anatase
(Degussa)
Fine Ti02, -250 nm, anatase
(Fisher Scientific)
Crystalline Si02, -800 nm
Subchronic inhalation
Nano-Ti02: 23.5 mg/m3; fine Ti02: 22.3 mg/m3; Si02 1 .3
mg/m3
6 hr/day, 5 days/wk for 3 months
6- or 12-month recovery before sacrifice
Lung burden: SiCt 0.32 mg immediately after exposure. Nano TiCWfine TiCfe
5. 33/6.62 mg, 4.15/1.2 mg, 3. 14/1. 66 mg immediately, 6 months, 12 months after
exposure, respectively.
6 months after exposure, in the lung: Si02 caused moderate focal interstitial fibrosis
and moderately severe focal alveolitis; nano Ti02 caused slightly less fibrosis and
fine Ti02 caused least fibrosis. Increases in stainable collagen in all three treated
groups, compared to untreated groups.
12 months after exposure, in the lung: Si02-treated rats showed decreased fibrosis;
nano Ti02 and fine Ti02 treated rats showed largely normal amount of interstitial
fibrosis but increases in alveolar macrophage number. Increases in stainable
collagen only in Si02.
Baggsetal. (1997)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Rat
[female CDF
(F344)/CrlBR]
Mouse
[female
B6C3F1/CrlBR]
Hamster
[female Syrian
golden
(LaklVG [SYR]
BR)]
FineTi02(DuPont),rutile;
aerosol 1.36- 1.44pm
MMAD
Nano-Ti02 (P25),
photocatalytic, average
primary particle size 21 nm,
1.37 |jm MMAD; aerosols:
1.29-1.44 |jm MMAD
Subchronic inhalation
Fine Ti02: 0,10,50 or 250 mg/m3
Nano-Ti02:0,0.5,2, or10mg/m3
6 hr/day, 5 days/wk for 13 weeks
0 (immediately after exposure), 4,13, 26, or 52 (up to 46
and 49 for hamsters exposed to fine Ti02 and nano-Ti02,
respectively) weeks of recovery before sacrifice
Lung burden of fine TiCt
  Immediately after exposure: lung burden offineTiCt mice> rats > hamsters at 50
  and 250 mg/m3: rats > mice > hamsters at 10 mg/m3. The lung burden decreased
  with time after exposure.
  The retention in lung-associated lymph nodes: rats > mice > hamsters at all
  concentrations.  The burden in the lymph  nodes increased with time after
  exposure (rats of all dose groups, mice of low and mid-dose groups, and hamsters
  of high-dose group).
  Pulmonary clearance kinetics of fine TiCt mice and  rats in high-dose groups
  retained 75% initial burden after 52 weeks of recovery, while hamsters retained
  only 10% initial burden after 26 weeks of  recovery. Overload in rats and mice at
  50 or 250 mg/m3.
Lung burden of nano-TiCfe
  Lung burden of nano-TiCfe rats a mice > hamster. Immediately after exposure, at
  10 mg/m3, rats and mice had same lung burdens for nano-TiCt At 2 or 0.5
  mg/m3, rats had more lung burden. Mice  and rats, but not hamsters, have
  pulmonary particle overload at 10 mg/m3.
  Pulmonary clearance kinetics of nano-TiCfe At 10 mg/m3, rats and mice  had linear
  fashion decreases of lung burden to -50% after 52-week recovery, while hamsters
  had a biphasic fashion decrease  to 3% after 48-week recovery. At 2 and 0.5
  mg/m3, rats, mice and hamsters had biphasic decreases in lung burn, and  rats
  only had detectable nano-TiCfe after the whole recovery period.
  Burden in the lymph nodes associated with lung: During the whole recovery time,
  burden increased with time in rats of 10 and 5 mg/m3 groups, and in mice of 10
  mg/m3 group. No nano-Ti02 was detected in hamster lymph nodes at any time
  point or treatment group.
General health of rats, mice and hamsters:
  Rats and mice at all treated groups had decreases in weight gain after exposure,
  and recovery occurred 3-4 week post exposure.  Mice exposed to 250 mg/m3fine
  Ti02 had a consistent lower weight during the recovery period, but rats exposed to
  250 mg/m3fme Ti02 had a consistent heavier weight. Hamster exposed to fine
  Ti02 had decreases in weight gain after exposure, and recovery 6 weeks post
  exposure. Hamsters exposed to  nano-Ti02 had weight loss after exposure and a
  slow recovery over the remainder of the study. Hamsters had higher morbidity
  and mortality rates across treatment groups than rats and mice; this was probably
  due to age-related renal diseases.
FineTiCfe Bermudez
etal.(2002)
Nano-TiCfe Bermudez
etal.(2004)
Comparison of fine
and nano-Ti02data
reported in Bermudez
etal. (2002) and
Bermudez etal.
(2004): Hextetal.
(2002, 2005)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
Testing Material
Treatment Conditions
Summary of Major Effects
Reference
(continuation
from previous
page)
                                                                          Pulmonary inflammation after fine TiCh exposure: Rats, mice and hamsters had
                                                                          pulmonary inflammation, and only hamsters had full recovery.
                                                                            Rats generally had more severe inflammation, and hamsters had the least.
                                                                            Fine Ti02 exposure: Increases in neutrophil %, lymphocyte %, and macrophage
                                                                            number in BAL fluid in rats and mice (in mid- and high-dose groups); increase in
                                                                            neutrophil % in rats at the lowest exposure. Hamsters had increased macrophage
                                                                            number, neutrophil %, and lymphocyte % at the highest concentration; they had
                                                                            an increased neutrophil % at the medium concentration. Within 26 weeks of
                                                                            recovery, hamsters showed normal neutrophil % and macrophage number; within
                                                                            46 weeks of recovery, hamsters had normal lymphocyte %.  Mice and rats showed
                                                                            partial recovery in neutrophil and macrophage response and no recovery in
                                                                            lymphocyte response after 52 weeks of recovery.
                                                                            Fine Ti02 exposure: LDH levels in BAL fluid transiently increased in mice and  rats
                                                                          Pulmonary inflammation after nano-Ti02 exposure: Rats and mice have pulmonary
                                                                          inflammation.
                                                                            Nano-Ti02 exposure: Rats and mice, but not hamsters, in the 10 mg/m3 groups
                                                                            have increased numbers of macrophage and neutrophil and concentrations of
                                                                            LDH and protein in BAL fluid.
                                                                          Pulmonary lesions are most severe in rats, and least in hamsters.
                                                                            Fine Ti02 exposure: Alveolar cell proliferation was seen in rats (0 week post
                                                                            exposure at mid- and high-dose groups, 4 and 13 weeks post exposure at high-
                                                                            dose group) and mice (13 and 26 weeks post exposure at high-dose group), but
                                                                            not in hamsters.
                                                                          Only rats had a progressive fibroproliferative lesion and alveolar epithelial
                                                                          metaplasia (bronchiolization).
                                                                            Fine Ti02 exposure: At 52 weeks post exposure, mouse lungs had particle-laden
                                                                            macrophages in alveolar and relatively normal alveolar septal structures. Rat
                                                                            lungs had particle-laden macrophages inside alveolar cells, fibrosis and thickening
                                                                            in interstitial tissue, and little alveolar epithelial metaplasia (bronchiolization) of
                                                                            lining epithelium.  Hamster lungs did not show retained particle burden or
                                                                            macrophage accumulation.
                                                                            Nano-Ti02 exposure: Alveolar epithelial proliferation, alveolar bronchiolization
                                                                            (alveolar epithelial proliferation of metaplastic epithelial cells around macrophages
                                                                            loaded with particles), alveolar septal fibrosis and interstitial particle accumulation
                                                                            in rats, but not mice nor hamsters, of the 10 mg/m3 group. With increasing time
                                                                            post exposure, the lesions became more severe.
                                                                          Species and particle differences:
                                                                            Overload was seen in rats and mice (but not hamsters) exposed to 50 and 250
                                                                            mg/m3 fine Ti02 or 10 mg/m3 nano-Ti02.
                                                                            Lung Ti02 burdens and tissue responses in mice, rat and hamsters exposed for
                                                                            13 weeks to 10 mg/m3  nano-Ti02 or to 50 mg/m3 fine Ti02 were similar for all three
                                                                            species.
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Table 5-6. Summary of health effects of nano-TiCh particles in mammalian animal models:  respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Rat
[female Wistar]


Mouse
[female NMRI]
Nano-Ti02 (P25),
photocatalytic, 80%
anatase/20% rutile, primarily
particle size 15-40 nm,
0.8 urn MMAD
Chronic inhalation
Rats: 24 month exposure: 7.2 mg/m3 for the first 4 months,
followed by 14.8 mg/m3 for 4 months, 9.4 mg/m3 for 16
months, and clean air for 6 months (concentration
sometimes are reported as 7.5,15, 10 mg/m3)
18 or 19 hr/day, 5 days/week in whole body chamber
Mice: 13.5 month exposure: Same treatment as in rats for
the first 8 months, followed by 9.4 mg/m3 for 5.5 months,
and clean air for 9.5 months
Rats:
  Increases in lung weight, and retention of inhaled nano-Ti02 in lungs and lung-
  associated lymph nodes (mean lung retention was 39.3 mg/lung at the end of
  exposure). The retention slowly decreased post exposure (from 40 mg/lung after
  18 months of nano-Ti02 exposure to 3.3 mg/lung at 4 months post exposure).
  Increased half-time of pulmonary clearance of tracer particles
     For inhaled 0.35 pm labeled tracer particles,
        After 3-, 12-, 18-month nano-Ti02 exposure and 18-month exposure plus
        3-month recovery, clearance half times were 208, 403, 357, and 368 days,
        respectively.
        The controls had 61-96 days for all time  points.
     For inhaled 3.5 pm labeled tracer particles,
        After 3-, 12-, 18-month nano-Ti02 exposure and 18-month exposure plus
        3-month recovery, clearance half times were 1222, 229, 58 and 48 days,
        respectively.
        The controls had 58-70 days for all time  points.
        The decreases in clearance half time after 12- and 18-month exposure,
        compared to controls, was possibly dye  to increases in lung weight, altered
        lung structure and breathing pattern, which lead to more in the tracheo-
        bronchial region of the long and apparently higher clearance rates.
Creutzenbergetal.
(1990)
                                                                                             Rats did not have increases in DMA adducts in the lung:
                                                                                               No increases in DNAadduct2 (nuclease Pi-sensitive adduct) in the lung.
                                                                                               Decreases in DMA adduct 1 (age-related, putative l-compound) in peripheral lung
                                                                                               DMA compared to filtered air-exposed rats, probably due to adduct dilution
                                                                                               through cell proliferation induced by particle exposure.
                                                                                                                                                      Gallagher etal. (1994)
                                                                                             Rats:
                                                                                               Increased mortality (60% vs. 42% in control) and lung wet weight, decreased
                                                                                               mean lifetime and body weight.
                                                                                               Increased incidence of lung tumors [18-month exposure: 5 out of 20 rats exposed
                                                                                               to Ti02 (0 out of 18  in control) had lung tumors. 24-month exposure: 4/9 rats in
                                                                                               Ti02 (0/10 in control)].
                                                                                             Mice:
                                                                                               No increase lung tumors.
                                                                                               Increased mortality (33% vs. 10% in control) and lung wet weight, decreased body
                                                                                               weight.
                                                                                             Carcinogenic in rats, but not in mice.
                                                                                                                                                      Heinrich etal. (1995)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                       Summary of Major Effects
     Reference
Mouse
[male C57BI/6]
Nano-Ti02 (Nanostructured
and Amorphous Materials),
anatase, measured average
primary particle size 3.5 ±
1.0 nm, BET surface area
219 ± 3 m2/g, surface
functionalization: 0, 0-H,
hbO (manufacturer reported
primary particle 5 nm,
surface area 210 m2/g)
Aerosol size geometric
mean 120-128 ±1.6-1.7 nm
for acute (two
concentrations) and
subacute (one
concentration) exposures
Acute inhalation
Doses-0, 0.77, or 7.22 mg/m3
Single exposure of 4 hours in whole-body chamber
No recovery time
No adverse effect/Minimal pulmonary inflammation.
  No treatment effects on most parameters measured to gauge inflammatory
  response (neutrophil number in BAL fluid, total protein, and LDH activity were not
  changed), and no effects on lung histopathology.
  Increased total cell count and macrophage count in BAL fluid at highest dose.
Grassian etal.
(2007b)
                                          Subacute inhalation
                                          Doses - 0 or 8.88 mg/m3
                                          4 hr/day for 10 days in whole-body chamber
                                          0,1, 2, or 3 wk of recovery before sacrifice
                                                     Moderate but significant pulmonary inflammatory response that lasted for at least 2
                                                     wk but resolved by wk 3 after exposure.
                                                       No changes in most parameters measured to gauge inflammatory response [total
                                                       protein, LDH activity, and cytokine (IFN-y, IL-6, or IL-1p) concentrations in BAL
                                                       fluid were not changed], and no effects on lung histopathology.
                                                       Increased macrophage count in BAL fluid in treated group at wk 0,1, and 2 post
                                                       exposure, but not at wk 3 post exposure.
                                                       Macrophages in BAL fluid were loaded with Ti02 particles, and less so at wk 3
                                                       post exposure.
Rat
[male Wistar]
Nano-Ti02 (Baker &
Collinson, Inc.), uncoated,
14% rutile/86% anatase,
hydrophobic surface,
average primary particle
25.1 ±8.2 nm (range 13-71
nm) measured under TEM.
BET surface area 51.1 ±0.2
m2/g.  Zeta potential was
16.5±2.2mVin1  mMKCI.
Aerosols: 0.7-1.1 urn MMAD
(geometrical standard
deviations 2.3-3.4).  Small
and large agglomerates in
the atmospheres, ranging
from below 100 nm to
several hundred nm.
Estimated number
concentrations of particles
<100 nm represents only
0.1-0.4% of the total particle
mass for all three
atmospheres.
Short-term inhalation
0, 2,10, and 50 mg/m3 (actual concentrations 0, 2.4,12.1,
and 50.0 mg/m3), 6 hr/day for 5 days, head-nose exposures
to dust aerosols
No recovery (immediately after the last exposure), 3- or 16-
day recovery after the last exposure. In other words,
necropsy on study days 5, 8, and 21, respectively.
Absolute lung weight was increased at 50 mg/m3 immediately after exposure, but not
after 16-day recovery.
Lung burden: 118.4, 544.9 and 1635 pg/lung immediately after inhalation of 2,10
and 50 mg/m3 nano-Ti02, respectively. 16 days of recovery later, the lung burdens
were 93.4, 400.4 and 1340 pg/lung, respectively. Calculated clearance half-times
were 47, 36 and 56 days for 2,10 and 50 mg/m3 groups, respectively.
In the mediastinal lymph nodes, Ti02 was only detected in the 50 mg/m3 group, and
the nano-Ti02 concentrations were higher at 16 days after the last exposure (mean
11.01  ug in collected lymph nodes) than immediately after exposure (mean 2.34 pg).
No Ti02was detected in the liver, kidney, spleen or basal brain with olfactory bulb
(detection limit 0.5 pg per organ).
BAL fluid: increases in total cell count at 50mg/m3 and polymorphonuclear
neutrophils at 10 mg/m3 and 50 mg/m3, but no changes in eosinophil, lymphocyte, or
macrophage counts, total protein content, enzyme activities, and levels of 9 (out of
tested 60) cell mediators. Among the 9 mediators, effects were only observed at
10 mg/m3 or higher immediately after exposure. After 3 days of recovery, effects
were still observed, but for clusterin and haptoglobin, they were observed at 2
mg/m3. Cell mediator levels were the same as controls after 16 days of recovery in
2 and 10 mg/m3 groups, but not in 50 mg/m3group.
Clinical pathology in blood: minor effects on serum cell mediator.  No increase in
serum troponin  I, a biomarkerfor myocardial damage in rodents.
Increased cell replication in large/medium bronchi and terminal bronchioles at all
three groups immediately after exposure and after 3 days of recovery (not after 16
days). Macrophage diffusion also decreases over time. No change in lung cell
apoptosis.
Changes were most prominent immediately after the last exposure or 3 days
afterward, and some endpoints returned to control levels by 16 days of recovery.
Ma-Hock etal. (2009)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Rat
[male F344]
Nano-Ti02, 20 nm, anatase
(Degussa); in aerosols:
agglomerates 0.71 ± 1.9|jm
MMAD
Fine TI02, 250 nm, anatase
(Fisher Scientific); in
aerosols: agglomerates
0.78±1.7|jmMMAD
Subchronic inhalation
Nano-Ti02: 23.5 ± 2.9 mg/m3; fine Ti02: 22.3 ± 4.2 mg/m3
6 hr/day, 5 days/week, for 12 weeks
Recovery for 4, 8,12, 29 or 64 weeks before sacrifice
Nano-Ti02 caused more severe and prolonged (~1 year) pulmonary inflammatory
response (i.e., increase in alveolar macrophages, polymorphonuclear neutrophils,
and lavagable protein) than fine Ti02.
When inflammatory response was expressed as number of polymorphonuclear
neutrophils and dose was expressed as surface area for retained particles (i.e.,
lavagable particles), nano-Ti02 and fine Ti02 shared the same dose response curve.
More severe and prolonged impairment of alveolar macrophage-mediated particle
clearance in  rats exposed to nano-Ti02 than rats exposed to fine TiCt Seven
months after Ti02 exposure, fine Ti02 exposed (but not nano-Ti02 exposed) rats
showed normal clearance rates.
Pathological changes in the lung: Nano-Ti02 caused greater epithelial effects (Type
II cell proliferation, occlusion of pores of Kohn) and  more interstitial fibrotic foci than
fmeTi02.
Dosimetry:
  Nano-Ti02 and fine Ti02 had a similar mass deposition in the lower respiratory
  tract and same retention in the alveolar space up to 1 year after exposure.
  Nano-Ti02 showed longer total pulmonary retention (retention halftime: -500 days
  for nano-Ti02, -170 days for fine TiCk), more translocation to the pulmonary
  interstitium and regional lymph nodes, a greater fraction being retained, and a
  larger fraction of alveolar burden in the interstitium (suggesting nano-Ti02
  depends mainly on mucocillary clearance, while fine-Ti02 depends on clearance
  to the gastrointestinal tract) than fine TiCt
Oberdorsteretal.
(1994)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Rat
[male Wistart
(strain
Crl:WI(Han)]
Nano-Ti02, 20-30 nm
(measured by TEM), 70%
anatase, 30% rutile, BET
surface area  48.6 m2/g,
uncoated, isoelectric point
(IEP)waspH7in10mM
KCI, MMAD 1.0 |jm  in
aerosol
Fine TI02, median size 200
nm in ethanol (measured by
DLS), rutile ,  BET surface
area6m2/g,IEP
-------
Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Rats
[female
Sprague-
Dawley
[Hla:(SD)CVF]]
Fine TI02, primary particle
<5 |jm, 99% rutile (reported
vendor), BET surface area
2.34 m2/g  [reported in
Sageretal. (2008)]
(Sigma-Aldrich, product #
224227) ;MMAD of the
aerosols 402 nm with a GSD
of2.4.CMD of the aerosols
710 nm
Nano-Ti02 (P25), primary
particle 21 nm.80%
anatase, 20% rutile
(reported by vendor), BET
surface area 48.08 m2/g
[reported in (Sageretal.,
2008)];MMADofthe
aerosols 138 nm  with a
GSD of2.2.CMD of the
aerosols 100nm
Short-term inhalation
Whole body chamber exposure
Exposures selected for not alter BAL markers of pulmonary
inflammation or lung damage
Exposure to fine TiCt aerosol concentration x exposure
time (actual deposition in lung)
  15mg/m3x480min(90|jg)
  16mg/m3x300 min (67 pg)
  12mg/m3x240min(36|jg)
  6mg/m3x240min(20|jg)
  3mg/m3x240min(8|jg)
Exposure of nano-TiCfe aerosol  concentration x exposure
time (calculated/actual deposition in lung)
  10 mg/m3x 720 min that took place over 3 days (38 pg)
  12mg/m3x240min(19|jg)
  6 mg/m3x240 min (10 pg)
  3mg/m3x480min(10|jg)
  12mg/m3x120min(10|jg)
  3mg/m3x240min(6|jg)
  1.5 mg/m3x240 min (4|jg)
Shame exposure (control): Omg/m3x240 min
24 h post exposure, sample collection, including
exteriorizing spintrapezius muscle with rats under
anesthesia while leaving its nerves supply and all feed
vessels intact for the test of arteriolar dilation
Histology of the lung:
   No significant inflammation.
   Particle accumulation in alveolar macrophage. Anuclear alveolar macrophages
   were seen in both nano-Ti02 and fine Ti02 exposed rats, but not in shame
   exposed rats. Anuclear alveolar macrophages are presumed to be an apoptotic
   change.
Endothelium-dependent arteriolar dilation as measured after intraluminal infusion of
the Ca2* ionophore A23187 in exteriorized spintrapezius muscle:
   Both fine Ti02 and nano-Ti02 exposures impaired arteriolar dilation in a dose-
   dependent manner, and nano-Ti02 exposure produced greater impairment than
   fine Ti02 at similar pulmonary load doses. No-effect dose of fine Ti02 was 8 pg
   (as in lung deposition), and for nano-Ti02 was 4 pg.
   On a mass base, nano-Ti02 was about one order of magnitude more potent than
   fine Ti02; on total particle surface area base calculated by BET surface area, fine
   Ti02 would be more potent than nano-Ti02 (the authors suspected
   overestimation of the total nano-Ti02 surface area delivered, since no
   agglomeration was considered).
   Additional nano-Ti02 exposure conditions (12 mg/m3x2h; 4mg/m3x6 h; 8
   mg/m3 x 3 h) yielded the same level of impairment of systemic arteriolar dilation,
   suggesting the response is dependent on the exposure concentration (of product)
   xtime.
Nurkiewiczetal.
(2008)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models:  respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                       Summary of Major Effects
     Reference
                                          Same exposure conditions as above (Nurkiewicz et al.,
                                          2008) for endogenous microcascuarl NO production tests,
                                          but only three groups in all other tests: aerosol
                                          concentration x exposure time (actual deposition in lung)
                                            Shame exposure (control): 0 mg/m3 x 240 min
                                            Fine Ti02:16mg/m3x 300 min (67 |jg)
                                            Nano-Ti02: 6 mg/m3x 240 min (10 |jg)
                                          24 hr post exposure, sample collection, including and
                                          exteriorizing spintrapezius muscle as described in
                                          (Nurkiewicz et al., 2008) and excising spintrapezius
                                          muscles from separate groups of rats for measurement of
                                          NO,  microvascualroxidative stress, and nitrotyrosin staining
                                                                                Same impairment of arteriolar dilation at 67 pg fine Ti02 and 10 pg nano-Ti02: more
                                                                                than 50% decrease compared to shame treated controls after Ca2* ionophore
                                                                                A23187 injection at 20 and 40 psi ejection pressures.
                                                                                No change in arteriolar dilation in  response to sodium nitroprusside (NO donor) in
                                                                                either 67 pg fineTiCkor 10 pg nano-TD2exposed rats, indicating no change in
                                                                                vascular smooth muscle sensitivity to NO.
                                                                                Increased ROS  amount in the microvascular wall in both 67 pg fine Ti02 and 10 pg
                                                                                nano-Ti02 groups at the same level as measured by ethidium bromide fluorescence.
                                                                                Increased nitrotyrosine expression in 10 pg nano-Ti02 treated rats (not measured in
                                                                                fine Ti02 group)  in lung (3 folds) and spinotrapezius microcirculation (4 folds), as
                                                                                compared to shame exposure, suggesting nitrosative injury in lung and systemic
                                                                                microcirculation.
                                                                                Decreased Ca2* ionophore A23187-stimulated endogenous microvascular NO
                                                                                production in fine Ti02 and nano-Ti02 treated groups in a dose-dependent manner:
                                                                                Similar to shame control, the NO production was sensitive to nitric oxide synthase
                                                                                inhibition caused by NG-monomethyl-l_arginine.
                                                                                Radical scavenging (by superoxide dismutase mimetic 2,2,6,6-tetramethylpiperidine-
                                                                                N-oxyl and catalase); inhibition of NADPHoxidase(byapocynin); and inhibition of
                                                                                myeloperoxidase (by 4-aminobenzoic hydrazide) all restored stimulated NO
                                                                                production and partially restored arteriolar dilation  (stimulated by Ca2* ionophore
                                                                                A23187) in 67 pg fine Ti02 and 10 pg nano-Ti02 groups.
                                                                                                                               Nurkiewicz etal.
                                                                                                                               (2009)
Instillations
Mouse
[male ICR]
Nano-Ti02 (Degussa), rutile,
highly dispersed and
hydrophilic fumed nano-
Ti02, diameter 19-21 nm
(average primary particle
size 21 nm), surface area of
50±15 m2/g, purity >99.5%
To avoid aggregation, the
nano-Ti02 suspension was
ultrasonicated before it was
used to treat animals or
cells; each sample was
vortexed just before an
aliquot was drawn for
instillation. However, authors
did not report the sizes of
aggregates before or after
sonication.
Single intra-tracheal instillation
0, 0.1, or 0.5 mg/mouse
3 days (for hyper-acute response), 1 wk (acute) or 2 wk
(chronic) of recovery before sacrifice
Gross morphology and histology of the lung: Emphysema-like lung injuries were
seen at 0.1 and 0.5 mg/mouse (more severe at 0.5 mg) at 3 days, 1 wk, and 2 wks
after the instillation.
  Pulmonary changes included disruption of alveolar space, alveolar enlargement,
  proliferation of alveolar type 11 pneumocyte,  increases in alveolar epithelial
  thickness, and accumulation of particle-laden macrophages.
  1 wk after instillation, 0.1 mg/mouse increased alveolar macrophage infiltration,
  type II pneumocyte proliferation, and apoptosis in macrophage and type II
  pneumocyte.
Gene expression in lung  1 wk after instillation of 0, 0.1, and 0.5 mg/mouse:
  cDNA microarray showed up-regulation in pathways involved in cell cycle
  regulation, apoptosis, chemokines, and complementary cascades.
  RT-PCR showed up-regulation in plgf, chemokines (cxclJ, cxc/5, and cc/3), tumor
  necrosis factor-related apoptosis-inducing ligand (TRAIL) and prostaglandin E
  receptor 4.
Western blot and ELISA showed increases in placenta growth factor (PIGF) protein
(a prechemokine that regulates the expression of several chemokines, leading to
inflammatory cascade) in cells and in serum.
Chen etal. (2006)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal  models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Rat
[female Wistar
(HsdCpb:WU)]
Nano-Ti02(P25),
photocatalytic, hydrophilic,
80% anatase/20% rutile,
primarily particle size 25 nm,
BET specific surface area
52 m2/g
Repeated weekly intra-tracheal instillation
Instilled doses:
  5 instillations x 3 mg
  5 instillations x 6 mg
  10 instillations x 6 mg
Increased primary benign tumors and malignant cancers in lung in all tested doses.
Mohretal. (2006)
Pott and Roller (2005)'
                Nano-Ti02 (Degussa T805 /
                P805),b crystal form not
                specified, coated with an
                organic silicon compound;
                21 nm;32.5m2/gb
                          Repeated weekly intra-tracheal instillation
                          Instilled doses:
                            15 instillations x 0.5 mg
                            30 instillations x 0.5 mg
                                                    High initial acute mortality lowered dose to 0.5 mg.
                                                    No conclusion on carcinogenicity.
                FineTi02, hydrophilic,
                anatase, primary particle
                200 nm, BET specific
                surface area 9.9 m2/g
                          Repeated weekly intra-tracheal instillation
                          Instilled doses:
                            10 instillations x 6 mg
                            20 instillations x 6 mg
                                                    Increased primary benign tumors and malignant cancers in lung in all tested doses.
Rat
[male Wistart]
Nano-Ti02, rutile, primary
particle diameter 4-6 nm, rod
shape (synthesized in the
lab by a soft chemistry
technique); BET surface for
instilled nano-Ti02 rods was
14.64 cm2 for dose of
1 mg/kg,82.30cm2for5
mg/kg.  Aggregates
appeared to be in a radial
arrangement and usually
less than 1  pm.
Single intra-tracheal instillation (acute effects)
1 or 5 mg/kg nano-TiCfe or vehicle only (150 pL)
Single intra-tracheal instillation
Nano-Ti02 was suspended in saline containing 0.01%
Tween 80 (a surfactant and emulsifier)
Blood collection and necropsy at 24 hours after instillation
Pulmonary inflammation: increases in macrophage and neutrophil numbers in BAL
fluid at 5 mg/kg. Most nano-Ti02 aggregates in BAL fluid were inside macrophages.
Pulmonary and cardiac edema: increases in the wet weight-to-dry weight ratios of
lung and of heart at 1 and 5 mg/kg.
Systemic inflammation: increases in monocyte and granulocyte (but not lymphocyte)
numbers in blood at 5 mg/kg.
Platelet aggregation: decreases platelet number in blood of rats exposed to 5 mg/kg
nano-Ti02, suggesting platelet aggregation  [in vitro supporting evidence: adding 2 or
10 |jg/ml_(butnot0.4|jg/mL) nano-Ti02 directly into untreated rat whole blood
caused platelet aggregation].
Nemmaretal. (2008)
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Table 5-6.    Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Rats
[male F344]
Nano-Ti02, -20 nm, anatase
Fine TI02, -250 nm, anatase
Single intra-tracheal instillation (acute effects)
500 |jg of either anatase nano-Ti02 or anatase fine Ti02
A single intra-tracheal instillation, followed by 24-hr
recovery
Anatase nano-Ti02 induced more inflammatory response and higher interstitial
access in the lung than anatase fine Ti02 of the same mass dose.
Oberdorsteretal.
(1992)
                Nano-Ti02, -20 nm, anatase
                (free anatase nano-Ti02)
                Alveolar macrophage
                collected 24 hrs after donor-
                rat received 200 pg anatase
                nano-Ti02 via intra-tracheal
                instillation (containing
                phagocytized anatase nano-
                Ti02)
                Alveolar macrophage
                collected from untreated rat
                lung
                PMNs from peripheral blood
                of untreated rats
                Serum-exposed anatase
                nano-Ti02 (incubated in rat
                serum for 1 hr and then
                washed twice)
                          Single intra-tracheal instillation (acute effects)
                          Free anatase nano-Ti02,104 pg
                          Phagocytized anatase nano-Ti02104|jg + 9.5x106
                          alveolar macrophages + 3.9 x 106 polymorphonuclear
                          neutrophils
                          Alveolar macrophages 6.8 x 106
                          Polymorphonuclear neutrophils 2.2 x 106
                          Serum-exposed anatase nano-Ti02100 pg
                          A single intra-tracheal instillation, followed by 24-hr
                          recovery
                                                    Free anatase nano-Ti02 and serum-exposed anatase nano-TD2 caused pulmonary
                                                    inflammatory reaction (same level) and interstitial distribution.
                                                    Phagocytized anatase nano-Ti02 alone did not contribute significantly to
                                                    inflammatory reaction, because the reaction can be explained by the alveolar
                                                    macrophages and polymorphonuclear neutrophils.
                                                    Phagocytized anatase nano-Ti02 showed less interstitial distribution than free
                                                    anatase nano-TiCt
                Fine Ti02, -250 nm, anatase
                Nano-Ti02, -20 nm, anatase
                FineTi02, -220 nm, rutile
                (from Dr. Siegal atArgonne
                National Laboratory,
                Argonne, IL)
                Nano-Ti02, -12 nm, rutile
                Carbon black, -30 nm
                (Cabot, 660R)
                          A single intra-tracheal instillation of 500 pg each; anatase
                          fine Ti02was also tested at 1000 pg; anatase nano-Ti02
                          was also tested at 65,107, 200, and 1000 pg
                          24-hr recovery
                                                    When inflammatory response was expressed as number of PMN and dose was
                                                    expressed as surface area for retained particles (i.e., lavagable particles), all
                                                    particles shared the same dose-response curve, except anatase and rutile nano-
                                                    Ti02athigh doses.
                                                    When inflammatory response was expressed as lavage protein and dose was
                                                    expressed as retained particle surface area, all particles shared the same dose
                                                    response curve.
                                                    Higher fractions of nano-Ti02 (anatase and rutile nano-TiCy were interstitialized
                                                    (translocated into interstitium or epithelium cells) than other particles.
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Table 5-6.  Summary of health effects of nano-TiCh particles  in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Rat
[strain / stock
not specified]
Nano-Ti02, -20 nm, surface
area is estimated to be 10
times of surface area of
-250 nm Ti02
Fine Ti02,-250 nm
Single Intra-tracheal instillation (acute effects)
Nano-Ti02:30,~150,500|jg
Fine Ti02: -150, 500, 2000 pg
Pulmonary inflammation (neutrophil % in lung lavage) was seen at 24 hr post
exposure. At the same mass dose, nano-Ti02 induced more inflammation than fine
Ti02. When doses are expressed as surface area, fine Ti02 and nano-Ti02 shared
the same dose-response curve.
Oberdorster (2000)
                Nano-Ti02
                Polytetrafluoroethylene
                (PTFE) (Teflon) fume, count
                median diameter-18 nm
                          Repeated inhalation of PTFE fume (5 x 105 particles/cm3 =
                          -50 pg/cm3, 5 min/day for 3 days) followed by a single
                          intra-tracheal instillation of 100 ug nano-Ti02
                                                    Cross tolerance: Nano-Ti02 induced less pulmonary inflammation (neutrophil % in
                                                    BAL fluid) in rats that had adapted to PTFE fumes for previous three days than in
                                                    rats that were not adapted (not exposed to PTFE fume). The author suggested this
                                                    cross tolerance is from adaptation to oxidative stress.
                Nano-Ti02, -20 nm
                Fine Ti02,-250 nm
                Lipopolysaccharide (LPS),
                an endotoxin found in gram
                negative bacteria
                          Inhalation of LPS followed by a single intra-tracheal
                          instillation of nano-TiCfe and fine Ti02 (acute effects)
                          LPS: -12 min exposure, -70 endotoxin units (estimated
                          alveolar dose)
                          Nano-Ti02 and fine TiCfe 50 pg
                          Within 30 minutes of inhalation of LPS or saline, intra-
                          tracheal instillation of nano-or fine Ti02
                          24 hours of recovery
                                                    LPS alone: mild pulmonary inflammation (-10% neutrophil in lung lavage at 24 hr
                                                    post exposure). The treatment of LPS was to mimic an early stage of infection with
                                                    gram negative bacteria (compromised host).
                                                    50 ug nano-Ti02, but not fine Ti02, further increased inflammatory response in
                                                    compromised hosts with mild pulmonary inflammation.
                                                      Neutrophil % in rats exposed to (LPS and then nano-TiCy > (LPS and then fine
                                                      Ti02J, LPS alone, nano-Ti02 alone > fine Ti02 alone, negative control.
                                                      It is unclear whether fine Ti02 at a dose that increases inflammatory response
                                                      would further increase inflammatory response in compromised hosts.
Rat
fWistarl
Nano-Ti02 (P25),
photocatalytic, 80%
anatase/20% rutile,
untreated, hydrophilic
surface, primarily particle
size -20 nm
Nano-Ti02 (Aeroxide®
T805),photostable, 80%
anatase/20% rutile,
silanized,
trimethoxyoctylsilane-treated
hydrophobic surface,
primarily particle size
-20 nm
Crystalline silica and quartz
particles (DQ-12) as positive
reference
Single intra-tracheal instillation (subchronic effects)
Doses: 0, 0.15, 0.3, 0.6, or 1.2 mg nano-Ti02 (positive
control: 0.6 mg quartz DQ12) in 0.2 mL saline
supplemented with 0.25% lecithin
3, 21, or  90 days of recovery
Transient pulmonary inflammatory responses to both types of nano-Ti02 (mostly only
at 1.2 mg dose, some at 0.6 mg groups) (most responses returned to normal by day
90).
P25 induced more pulmonary inflammatory responses than T805 in some tests, but
T805 induced more proliferation changes in the lung (as percentage of Ki67-positive
cells) than P25 on days 3 and 21.
Neither P25 nor T805 increased oxidative DNA adduct (as 8-oxoguanine) in the lung
on day 90.
Quartz induced persistent inflammatory response and increased 8-oxoguanine on
day 90.
Rehnetal. (2003)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models:  respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                       Summary of Major Effects
     Reference
Rat
[male Wistar]
Nano-Ti02 (Degussa), mean
diameter 29 nm, BET
surface area 49.78 m2/g
Fine Ti02 (Tioxide Ltd),
mean diameter 250 nm BET
surface area 6.6 m2/g
Carbon black,  mean
diameter 260.2 nm, BET
surface are 7.9 m2/g
Ultrafme carbon black, mean
diameter 14.3  nm, BET
surface 253.9  m2/g
Single intra-tracheal instillation (acute effects)
0,125, and 500 ug particles in saline
24 hours of recovery before sacrifice
Nano-Ti02 at 500 ug (but not nano-Ti02 at 125 ug or fine Ti02 at either 125 or 500
ug) increased neutrophil number (inflammation), LDH activity (cytotoxicity), GGT
activity (epithelial damage), total protein in bronchoalveolar lavage fluid (membrane
permeability), and macrophage activity to migrate toward chemotaxin C5a
(chemotaxis).
Both nano- and fine Ti02 (at 500 ug, but not at 125 ug) decreased phagocytic
function of macrophage.
Carbon black caused same changes as fine Ti02, with the  exception of increases in
LDH activity at 500 ug.
Ultrafme carbon black caused same changes as nano-Ti02, but increases in
inflammation and LDH and GGT activities were significant  at 125 ug (nano-Ti02
caused significant changes at 500 ug only).
Renwicketal. (2004)
Rat
[male
Crl:CD(SD)IGS
BR]
Fine Ti02 (DuPont): primary
particle -300 nm, anatase,
-99 wt % TI02/-1 wt %
alumina, BET surface area
~6m2/g(R-100)
Nano-Ti02 rods (synthesized
hydrothermally):  primary
particle length 92 - 233 nm x
width 20 - 35 nm, anatase,
BET surface area 26.5 m2/g
Nano-Ti02 dots (synthesized
hydrothermally):  primary
particle diameter 5.8-6.1
nm, sphere, anatase, BET
surface area 169.4m2/g
Quartz (Min-U-Sil quartz):
median primary particle
~1.5|jm (range 1 -3 pm),
crystalline silica, BET
surface area 4 m2/g
Single intra-tracheal instillation (subchronic effects)
0,1 or 5 mg/kg of each testing material in PBS with polytron
dispersant
BAL fluid analysis at 24 hr, 1 week, 1 month, and 3 months
post exposure (5 rats per group per dose per time point)
Morphological studies at the same time points (4 rats per
group per high dose per time point; 4 rats per group per low
dose for the first two time points)
Like fine Ti02, nano-Ti02 rods and nano-Ti02 dots caused only transient pulmonary
inflammation, and not significant lung toxicity.
  All 5 mg/kg Ti02 (fine, nano rods, and nano dots), but not 1 mg/kg Ti02, caused
  transient, short-lived inflammation (increases in neutrophil % in BAL fluid at 24 hr
  post exposure only; increases in LDH by 5 mg/kg nano-Ti02 rods at 24 hr post
  exposure only).
  No changes in lung weight, tracheobronchial cell proliferation (measured in high
  dose groups only) or lung morphology (pathological changes).
  Ti02 in macrophages was seen in all three types of Ti02.
  Transient lung parenchymal cell proliferation in low and high fine Ti02 at 1 week
  post exposure (different from previous studies in similar conditions).
Quartz caused sustained pulmonary inflammation and early sign of pulmonary
fibrosis.
  Sustained pulmonary inflammation (increases in neutrophil % in BAL fluid at
  1 mg/kg  at 24 hr after exposure, 5 mg/kg at all time points) (increases in LDH at
  5 mg/kg  at all time points) (increase in neutrophils and foamy alveolar
  macrophages).
  Prelude  of fibrosis (thickening of lung tissue) (persistent lung parenchymal cell
  proliferation at 5 mg/kg at 1 month and 3 month post exposure).
  Absolute lung weight was increased at 5 mg/kg at 1 wk, 1  month, and 3 months
  post exposure.  Increased tracheobronchial cell proliferation at 5 mg/kg (not
  measured in low dose) at 24 hr post exposure only.
Warheitetal. (2006)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
    Reference
Rat
[Crl:CD®(SD)IG
SBR]
Nano-Ti02 (DuPont),
photostable, rutile, coated
with alumina, (-98% titanium
dioxide, -2% alumina),
average particle size of 136
nm in water and average
BET surface area of 18.2
m2/g(uf-1)
Nano-Ti02 (P25) (Evonik),
photocatalytic, 80%
anatase/20% rutile, not
coated, average particle size
129.4 nm  in water, average
BET surface area 53.0 m2/g
Nano-Ti02 (DuPont),
photostable, rutile, coated
with silica and alumina
surface coating (-88 wt %
titanium dioxide, ~7wt%
amorphous silica and ~5 wt
% alumina),  average particle
size of-149.4 nm in water,
average BET surface area
35.7 m^/g (uf-2)
Fine Ti02 (DuPont),
photostable, rutile, coated
with alumina (-99% titanium
dioxide and-1% alumina),
an average particle size 382
nm in water, average BET
surface area 5.8 m2/g
Quartz
Single intra-tracheal instillation (subchronic effects)
0,1, or 5 mg/kg
90 days recovery period
No sustained adverse pulmonary effects for photostable nano-Ti02(both types of
coated rutile).
Pulmonary inflammation and cytotoxic effects at highest exposure of photocatalytic
nano-Ti02 increased bronchoalveolar lavage fluid LDH and BAL fluid microprotein
concentrations.
Increased tracheobronchial and lung parenchymal cell proliferation rates at highest
exposure of photocatalytic nano-Ti02.
Lung inflammation/cytotoxicity/cell proliferation and histopathological responses:
quartz > nano-Ti02 P25 (anatase and rutile) > fine Ti02 (rutile) = nano-Ti02 uf-1
(rutile) = nano-Ti02 uf-2 (rutile).
Warheitetal. (2007a)
Warheitetal. (2007c)
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Table 5-6.  Summary of health effects of nano-TiCh particles in mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                       Summary of Major Effects
     Reference
Mouse
[female
CD1(ICR)]
Nano-Ti02 (Hangzhou
Dayang Nanotechnology Co.
Ltd.),rutile, 80nm,
measured average size 71.4
± 23.5 nm, purity >99%
FineTi02(Zhonglian
Chemical Medicine Co.),
anatase, 155 nm,  measured
average size 155.0 ± 33.0
nm, purity >99%
Repeated intranasal instillation
-500 |jg Ti02 in pure water per mouse very other day for 2,
10, 20, or 30 days (1, 5,10 or 15 instillations, respectively)
Necropsy 1 day after last instillation
For translocation of Ti02 into brain: 6 mice per group for
each time point.
For effects in brain: 10 mice per group
Ti02 distribution (measured after 15 instillations): first into olfactory bulb, and then to
hippocampus. Ti concentrations: hippocampus, olfactory bulb > cerebellum,
cerebral cortex > thalamus.
Serum biomarkers for liver function (ALT, AST, ALP), kidney function and cholesterol
levels: No consistent change. Only changes were increased ALT (80 nm group after
1 and 5 instillations, 155 nm group after 5 instillation), increased AST (80 nm group
afterS instillations) and increase ALP (155 nm group after 1 instillation).
Pathological changes in kidney: atrophy of renal glomerulus, infiltration and
dwindling of interstitially inflammatory cells in the lumen of Bowman's capsules.
No changes in organ weight. No pathological changes in heart, liver, spleen,
cerebral cortex or cerebellum.  No change in proinflammatory cytokine TNF-a in
serum.
Brain:
  Oxidative stress: GSH-Px and GST activities and GSH levels were increased in
  the 80 nm group after 5 instillations, but not in other groups or other time points.
  Malondiadehyde levels (indicator for lipid peroxidation) and soluble protein
  carbonyl content (indicator for protein oxidation; measured only after 15
  instillations) were increased in both 80 and 155 nm group after 15 instillations.
  SOD activity was decreased in 155 nm after 15 instillations.  Catalase activity
  (measured only after 15 instillations) was increased in 80 and 155 nm groups.
  Pathological changes in olfactory bulb and C1A regions of hippocampus: Olfactory
  bulbs showed increased neuron numbers, irregular arrangement of neuron cells,
  and ultra-structural changes  in both 80 and 155 nm groups.  CA1 region of the
  hippocampus showed enlarged and elongated pyramidal cell soma, dispersed
  arrangement and loss of neurons, fewer Nissl bodies, fewer  mitochondria, and
  increased rough endoplasmic reticulum.
  Astrocytes may be damaged (only measured after 15 instillations): Hippocampus
  had increased glial fibrillary acidic protein (GFAP) levels, particularly in CA4
  region. Activity of cholinesterase (which inactivates acetylcholine, a
  neurotransmitter) was increased. Both changes were in 80 and 155 nm groups.
  Neurotransmitters: Levels of glutamic acid (a neurotransmitter) and nitric oxide
  (NO, as neurotransmitter and from inflammatory response) were increased in both
  80 and 155 nm groups (measured only after 15 instillations).
  Cytokines: Increased THF-a  and IL-1|3, but not IL-6(155 nm  after 15 instillations).
Wangetal. (2008a)
Wangetal. (2008b)
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Table 5-6. Summary of health effects of nano-TiCh particles in  mammalian animal models: respiratory route (continued).a
   Animal
    Testing Material
              Treatment Conditions
                      Summary of Major Effects
     Reference
Mouse
[CD-1(ICR)]
Nano-Ti02 (Hangzhou
Dayang Nanotechnology Co.
Ltd.), rutile, 25 nm, purity
>99%
Nano-Ti02 (Hangzhou
Dayang Nanotechnology Co.
Ltd.), rutile, 80 nm, purity
>99%
FineTi02(Zhonglian
Chemical Medicine Co.),
anatase, 155nm, purity
>99%
Repeated intranasal instillation (subacute effects)
10 pLof50 mg/kg Ti02or water every two days
Blood and brain were collected from anesthetized mice
after 2, 10, 20, or 30 days
No changes in water and food consumption or body weight.
Brain Ti02 content (measured in all brain samples): increased in treated mice and
was highest in 25 nm treated group at 2 and 10 days; decreased slightly and was
similar in all treated groups at 20 and 30 days.
Neurotransmitters (measured in 20 and 30 day brain samples):
  Changed in 80 nm and 155 nm TiCVtreated mice compared to control, but not in
  25 nm Ti02-treated mice. All changes were after 20 days, with the exception of
  decreased dopamine in 80 nm group after 30 days.
  After 20 days: Norepinephrine was significantly increased in 80 and 155 nm Ti02-
  treated mice; 5-hydroxytryptamine was significantly increased in 155 nm Ti02-
  treated mice; homovanillic and 5-hydroxyindole acetic acid were decreased in 80
  and 155 nm TiCVtreated mice; dopamine was decreased in 80  nm Ti02-treated
Wangetal.(2007b)
a ALP-Alkaline phosphatase, a marker of type II epithelial cell toxicity (Ma-Hock etal., 2009) or liver toxicity
  ALT - Alanine transaminase
  AST - Aspartate aminotransferase
  BAL - Bronchoalveolar lavage
  BET - Brunauer, Emmett, Teller method of calculating surface area
  CMD - Count median diameter
  DLS - Dynamic light scattering
  ELISA - Enzyme-linked immunosorbent assay
  F344-Fischer 344
  GFAP - Glial fibrillary acidic protein
  GGT-y-glutamyltransferase, a marker for damage to Clara and type II epithelial cells (Ma-Hock etal.,
  2009)
  GSD - Geometric standard deviation
  GSH - Reduced glutathione
  GSH-Px - Glutathione peroxidase
  GST - Glutathione-S-transferase
  lEP-lsoelectric point
  IL-1p-lnterleukin-1 beta
b According to Pott and Roller (2005): "Titanium dioxide T 805 from Degussa was ordered from Sigma-Aldrich, but the supplier only offered an amount of at least 40 kg P805.  Neither Sigma-Aldrich nor Degussa
  answered at all clearly when questioned insistently as to the difference between T 805 and P 805.  So, it is not proven that P 805 is identical with T 805 from Degussa."  The primary particle size and surface area in the
  table were from Pott and Roller (2005).  Currently available T805 is photostable nano-Ti02 (80% anatase, 20% rutile) that has been treated with octylsilane to achieve a hydrophobic surface. Degussa T805 primary
  particle is still 21 nm, but specific surface area (BET) is 45 m2/g (Llames, 2008a).
                                                                             IL-6-lnterleukin-6
                                                                             IFN-y- interferon-gamma
                                                                             LDH - Lactate dehydrogenase, a general marker of cell injury (Ma-Hock et al., 2009)
                                                                             LPS - Lipopolysaccharide
                                                                             MMAD - Mass median aerodynamic diameter
                                                                             MTP - Microsomal triglyceride
                                                                             NADPH - Nicotinamide adenine dinucleotide phosphate
                                                                             P25-AEROXIDE®P25
                                                                             PBS - Phosphate buffered saline
                                                                             PIGF - Placenta growth factor
                                                                             PMN - Polymorphonuclear neutrophils
                                                                             PTFE - Polytetrafluoroethylene
                                                                             ROS - Reactive oxygen species
                                                                             RT-PCR - Real-time polymerase chain reaction
                                                                             SOD - Superoxide dismutase
                                                                             TEM - Transmission electron microscopy
                                                                             TNF-a - Tumor necrosis factor-alpha
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 1    5.3.1.2.4. Toxicity by Other Exposure Routes

 2          Ocular exposure, intravenous injection, and subcutaneous injection have also been investigated in
 3    nano-TiO2 toxicity studies (Table 5-7). Ocular exposure to sunscreen containing nano-TiO2 could occur
 4    accidentally when sunscreen spray and sunscreen lotion are applied.  At least one brand of sunscreen
 5    lotion that contains nano-TiO2 is in a tear-free formula and marketed for children (Project on Emerging
 6    Nanotechnologies, 2007). A single ocular exposure to photocatalytic nano-TiO2 caused conjunctival
 7    redness for 1 or 2 days in rabbits (Warheit et al., 2007a).
 8          One journal article and two professional meeting abstracts are available on the effects of injected
 9    nano-TiO2 in rats and mice. In the Fabian et al. (2008) study, an intravenous injection of 5 mg/kg nano-
10    TiO2 with unknown photoreactivity did not induce changes in blood tests diagnostic for inflammatory
11    responses, kidney toxicity, or liver toxicity. Two meeting abstracts presented immunological effect
12    studies in mice exposed to nano-TiO2 through subcutaneous and intravenous injections (Miller et al.,
13    2007b; Weaver et al., 2007). Preliminary results  showed that photocatalytic nano-TiO2 in suspension
14    (Degussa W740X) appeared to have very limited inflammatory ability, and very high doses (560 mg/kg
15    for intravenous injections and  5,600 mg/kg for subcutaneous injections) were needed to produce
16    immunological effects (Weaver, 2008).
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Table 5-7.        Summary of health effects of nano-TiCh particles in mammalian animal models: other (injection, ocular) route.a
Animal
Testing Material
Treatment Conditions
Summary of Major Effects
Reference
Injection
Rat
[male Wistar
(strain
Crl:WI(Han)]
Nano-Ti02, primary particle
20-30 nm (measured by
TEM), BET surface area
48.6m2/g,70%anatase/
30% rutile, uncoated, IEP
waspH7in10mMKCI


Fine Ti02 (Kronos
International), median size
200 nm in ethanol
(measured by DLS), rutile,
BET surface area 6 m2/g,
IEP spleen » lung >
kidney. The time for the Ti02 concentration to return to normal levels were in the
same sequence. Liver had same Ti02 levels after 14 and 28 days. Spleen had
slightly decreased Ti02 levels 14 and 28 days after injection. Lung and kidney had
no elevated Ti0214 days after injection.  No Ti02 detected in blood cells, plasma,
brain or lymph nodes (mediastinal, mesenteric, popliteal) at any three time points
tested (detection limit 0.3 pg Ti = 0.5 pg Ti02 per tissue).
Fabian etal. (2008);
van Ravenzwaayetal.
(2009)
Mouse
[Balb/C]
Nano-Ti02 (Degussa
W740X), dispersion of
photocatalytic uncoated
nano-Ti02 (80% anatase/
20% rutile) at 40 wt%,
primary particle 4.7 nm,
mean aggregate size s 100
nm; (Evonik, 2008; Llames,
2008b; Weaver, 2008)
Intravenous injections
5.6 mg/mouse/day for 2 days (total dose 11.2 mg/mouse)
1 or 3 days of recovery before sacrifice
Lung, liver, and spleen showed white discoloration and phagocytosis of nano-Ti02
aggregates by macrophages under light microscope.
Miller etal. (2007b)
Mouse
[sex,
strain/stock not
specified]
Nano-Ti02 (Degussa
W740X), dispersion of
photocatalytic uncoated
nano-Ti02 (80% anatase/
20% rutile) at 40 wt%,
primary particle 4.7 nm,
mean aggregate size s 100
nm; (Evonik, 2008; Llames,
2008b; Weaver, 2008)
Subcutaneous injections: total 0 or total 5600 mg/kg over
two days
Intravenous injections: total 0 or total 560 mg/kg over two
days
1 or 5 days of recovery
Subcutaneously injected mice:
  Day 1: No changes in any cell population in peripheral blood, except CD8+ T cells.
  Day 5: Increases in granulocytes in circulation and spleen; decreases in
  circulating lymphocyte percentages; no changes in macrophage percentages or
  any cell population in draining lymph nodes.
  Lack of Con-A stimulated T-cell proliferation in lymph nodes.
Intravenously injected mice:
  Macrophage in the marginal zone of the spleen white pulp contained nano-Ti02
  aggregates, suggesting interaction between T-cells and nano-TiCt
  No changes in Con-A stimulated T-cell proliferation.
Weaver etal. (2007)
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Table 5-7.  Summary of health effects of nano-TiCh particles in mammalian animal models: other (injection, ocular) route (continued).a
Animal
Testing Material
Treatment Conditions
Summary of Major Effects
Reference
Occular exposure
Rabbit
[male New
Zealand White]
Nano-Ti02, (P25),
photocatalytic, 80%
anatase/20% rutile, not
coated, average particle size
was 129.4nm in water,
average BET surface area
was 53.0 m2/g (Warheit,
pers. comm., 2008b)
Acute ocular irritation
Doses - 0 or 57 mg to one eye of each animal
Single exposure (the eye remained unwashed following
treatment)
Observation at 1, 24, 48, and 72 hours following
administration of the nano-Ti02
Reversible conjunctival redness in the treated eye (normal by 24 or 48 hours after
administration of nano-TiC^).
No corneal injury evident, no clinical signs observed, and no body weight loss
occurred.
Warheit etal. (2007a)
a  BET - Brunauer, Emmett, Teller method of calculating surface area
  BW-Body weight
  CDS - Cluster of differentiation 8
  CDS + T cell - Cytotoxic T cell with CDS surface protein
                                                                         DLS - Dynamic light scattering
                                                                         IEP- Isoelectric point
                                                                         P25-AEROXIDE®P25
                                                                         TEM - Transmission electron microscopy
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      5.3.1.3. Summary of Non-carcinogenic Effects
 1          Some of the non-carcinogenic effects shared by conventional and nano-TiO2 were similar in the
 2    nature or type of the effects, but differed in dose-response. For example, pulmonary inflammation in
 3    laboratory animals and overload in rats were observed after respiratory tract exposures to either
 4    conventional TiO2 or nano-TiO2, and nano-TiO2 often caused more severe or more persistent responses
 5    than conventional TiO2 at the same mass concentrations/doses. Systemic effects were also observed:
 6    increased inflammatory cell numbers and decreased platelet numbers in the blood, renal pathology,
 7    potential hepatic toxicity, and changes in the brain morphology and neurotransmitters. Except for the
 8    effects in the brain, the aforementioned effects outside the lung have been reported only once and have
 9    not been confirmed by other laboratories. While topically applied photostable nano-TiO2 is not expected
10    to cause adverse effects in healthy skin, data are lacking on the effects in healthy flexed human skin and
11    damaged human skin.
      5.3.2. Carcinogenic Effects
12          The carcinogenicity of TiO2 to humans has been reviewed by various international health
13    organizations and workplace regulatory agencies. Currently, TiO2 (including nano-TiO2, but not
14    considered separately) is classified as "possibly carcinogenic to humans" (Group 2B) by the International
15    Agency for Research on Cancer (IARC) (Baan, 2007; IARC, 2006) and as "carcinogenic" (Class D2A) by
16    the Workplace Hazardous Materials Information System (WHMIS), a program administered by the
17    Canadian Centre for Occupational Health and Safety (CCOHS) (2006).
18          In a 2005 draft evaluation, TiO2 was not designated as a "potential occupational carcinogen," due
19    to insufficient evidence (NIOSH, 2005). For nano-TiO2, NIOSH expressed concern in the 2005 draft
20    about the potential carcinogenicity of ultrafine TiO2 (nano-TiO2) if exposure levels were at the current
21    mass-based occupational limits of 1.5 mg/m3 for respirable dust or 15 mg/m3 for total dust, and
22    recommended controlling exposure to as low as feasible below the recommended exposure limit (NIOSH,
23    2005).  Based on calculated lung cancer risks, NIOSH (2005) stated a draft recommendation for an
24    exposure limit of 0.1 mg/m3 for ultrafine TiO2, which is more than 10-fold lower than the exposure limit
25    of 1.5 mg/m3 for fine TiO2 (less than 2.5 (im), as time-weighted average concentrations for up to 10 hr/day
26    during a 40-hour work week.
27          This section reviews studies in humans and in animals on carcinogenicity of nano-TiO2 and briefly
28    discusses the mode of action of conventional TiO2 and nano-TiO2 carcinogenicity.  Conventional TiO2has
29    been shown to induce lung cancer through inhalation in rats at 250 mg/m3 (6 hr/day, 5 days/week for 24

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 1    months) (Lee et al., 1985a, 1985b), but not at 50 mg/m3 or below (Lee et al., 1985a, 1985b; Muhle et al.,
 2    1991). No increases in tumors were observed in mice receiving a single intra-tracheal instillation of 0.5
 3    mg of TiO2, in mice and rats fed with TiO2 in the diet at up to 5.0% daily for 103 weeks, or in hamsters
 4    given 3 mg of TiO2 via intra-tracheal instillation weekly for 15 weeks (Baan, 2007).  Similarly,
 5    epidemiological studies did not show increased lung cancer in people exposed to conventional TiO2
 6    (Boffetta et al., 2001; Boffetta et al., 2004; Chen and Fayerweather,  1988; Fryzek et al., 2003;
 7    Ramanakumar et al., 2008; Siemiatycki, 1991). The carcinogenicity studies of conventional TiO2 are not
 8    discussed in detail in this case study, and readers are referred to studies cited here and in the IARC
 9    Monographs Working Group report (Baan, 2007).

      5.3.2.1. Studies in Humans
10          Seven epidemiological studies of TiO2 carcinogenicity have been reported: two population-based
11    case-control studies (one for lung cancer (Boffetta et al., 2001) and the other for 20 types of cancer
12    (Siemiatycki, 1991)); two retrospective cohort mortality studies (Boffetta et al., 2004; Fryzek et al.,
13    2003); one mortality,  morbidity, and case-control study (lung cancer and chronic respiratory diseases)
14    (Chen and Fayerweather, 1988); and two case-control studies (lung cancer) (Ramanakumar et al., 2008).
15    Based on these studies, IARC (2006), the Canadian Centre for Occupational Health and Safety (CCOHS)
16    (2006), and NIOSH (2005) concluded that the evidence is insufficient to conclude that TiO2 exposure
17    increases the risk of lung cancer in human beings.  Furthermore, none of these studies were designed for
18    nano-TiO2 exposure, and none of them provided information on TiO2 particle sizes.  The risks posed by
19    nano-TiO2 (ultrafine primary particles) and the relationship between particle size and lung cancer risk in
20    humans cannot be discerned from these studies.

      5.3.2.2. Animal Studies
21          Carcinogenicity of nano-TiO2 was observed in three animal studies using photocatalytic nano-TiO2
22    in rodents (Borm et al., 2000; Heinrich et al., 1995; Pott and Roller,  2005). Increased lung tumor
23    incidences were observed in rats (Borm et al., 2000; Heinrich et al.,  1995; Pott and Roller, 2005), but not
24    in mice (Heinrich et al., 1995), exposed to P25 through inhalation or intra-tracheal instillation.
25    Photocatalytic nano-TiO2 given through intraperitoneal injections did not increase tumors in the
26    abdominal cavity in rats (Pott et al., 1987). Intramuscular implantation of nano-TiO2 with unknown
27    photo-reactivity also did not increase tumors at the sites of implantation in rats (Hansen et al., 2006).
28    Data specifically on photostable nano-TiO2 carcinogenicity are inconclusive (2005).
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 1    5.3.2.2.1. Intratracheal Instillation
 2          Female Wister CRP/WU rats received fine and nano-TiO2 via intra-tracheal instillations, and the
 3    tumor incidence and pulmonary inflammation were measured 2.5 years after administration (Borm et al.,
 4    2000).  Fine TiO2 (250 nm) was given 6 times at 10 mg each, and the photocatalytic nano-TiO2 (21 nm,
 5    80% anatase, 20% rutile, uncoated, P25) was given 5 times at 6 mg each (Borm, pers. comm, 2008). At
 6    these total doses (60 mg for fine TiO2 and 30 mg for nano-TiO2), lung clearance might be expected to be
 7    severely compromised. The authors found evidence of alveolar and interstitial inflammation 2.5 years
 8    after instillation.  The histologically confirmed tumor incidences were 27% for fine TiO2 and 66% for
 9    nano-TiO2, while the macroscopic tumor incidences were only 20.9% for fine TiO2 and 50% for nano-
10    TiO2. In vehicle-treated controls, the microscopic tumor incidences were between 5 and 6%. Although
11    particles that induce high tumor incidences generally also cause high inflammatory cell counts, nano-TiO2
12    caused a high tumor incidence and low inflammatory cell counts. Borm et al. (2000) suggested that
13    tumor formation was directly related to high interstitialization rather than overload and subsequent tissue
14    response, similar to the premise that lung burden is correlated to surface area of the particles (Oberdorster
15    etal., 1994).
16          Pott and Roller (2005) reported increases in  pulmonary tumors in rats exposed to hydrophilic fine
17    TiO2 and hydrophilic nano-TiO2, but were unable to draw conclusions about the carcinogenicity of
18    hydrophobic nano-TiO2. Female Wistar (HsdCpb:WU) rats received weekly intra-tracheal instillations of
19    three types of TiO2:  hydrophilic nano-TiO2 (P25),  hydrophobic nano-TiO2 (Aeroxide® P805/Degussa
20    P805, see Footnote c in Table 5-8), and hydrophilic fine TiO2 (232033 from Sigma).  If the products used
21    in the study are the same as those currently available, both the hydrophilic nano-TiO2 and fine TiO2 were
22    photocatalytic and the hydrophobic nano-TiO2 was photostable.  The tested TiO2 physicochemical
23    properties, doses, and key results are listed in Table 5-8. The types of primary benign lung tumor were
24    adenoma and epithelioma, and the primary malignant tumors were adenocarcinoma and squamous cell
25    carcinoma. At the tested doses, 42-46 rats out of 48 rats/group survived in the hydrophilic nano-TiO2 and
26    hydrophilic fine TiO2 groups, and statistically significant increases in benign or malignant lung tumors, or
27    both, were observed in these two groups.
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Table 5-8
Treatment
Nano-Ti02,
hydrophilic
(P25)
Nano-Ti02,
hydrophobic
(Degussa
P805)'
(Sigma AL
900032)'
FineTi02,
hydrophilic
(Sigma AL
232033)
No
treatment
. Treatments and pulmonary tumor incidences in rats exposed to fine and nano-TiCh
through intra-tracheal instillation in Pott and Roller (2005) study.
Crystal form;
primary
particle size;
specific
surface area
(BET)
Majority
anatase; 25
nmb(21 nm
and 30 nm
were also
reported); 52
m2/g
(Data of
Degussa
T805)c Crystal
form not
specified,
coated with an
organic silicon
compound; 21
nm; 32.5 m2/g
Anatase; 200
nm; 9.9m2/g
-
Photo-
stability
Photo-
catalytic
(Currently
available
Degussa
T805 is a
photostable
UV filter)
(Untreated
anatase is
photo-
catalytic)
-
Dose
(number of
instillations
x mg per
instillation)
5x3.0
5x6.0
10x6.0
15x0.5
30x0.5
10x6.0
20x6.0
-
Rats at
start/at
risk3
48/42
48/46
48/46
24/11
48/15
48/44
48/44
48/46
Survival
50%
(wks)
114
114
104
86
114
108
113
113
Lungs with Lungs with
primary primary
benign malignant
tumors (%) tumors (%)
21.4 31.0
17.4 50.0
23.9 45.7
0.0 0.0
6.7 0.0
15.9 13.6
38.6 25.0
0.0 0.0
Lungs
with
tumors,
total (%)
52.4
67.4
69.6
0.0
6.7
29.5
63.6
0.0
Lungs with
metastases
of other
tumors (%)
14.3
15.2
15.2
9.1
6.7
11.4
2.3
13.0
        BET - Brunauer, Emmett, Teller method of calculating surface area
        P25-AEROXIDEOP25
        UV-Ultraviolet (light/radiation), wavelengths in the range of 10-400 nm
        a Rats at risk were "sufficiently examined rats which survived at least 26 weeks after first instillation" according to Pott and Roller (2005).
        b Regarding particle characteristics, Pott and Roller (2005) noted "There are no clearly measured values or more than one piece of information." The value
          listed in the table was assumed to be close to the correct value and was used for further calculations by Pott and Roller (2005).
        'According to Pott and Roller (2005): "Titanium dioxide T805 from Degussa was ordered from Sigma-Aldrich, but the supplier only offered an amount of at
          least 40 kg P 805. Neither Sigma-Aldrich nor Degussa answered at all clearly when questioned insistently as to the difference between T805 and P805.
          So, it is not proven that P805 is identical with T805 from Degussa." The primary particle size and surface area in the table were from the Pott and Roller
          (2005) study. Currently available T805 is photostable nano-Ti02 (80% anatase, 20% rutile) that has been treated with octylsilane to achieve a hydrophobic
          surface. The primary particle size is still 21 nm, but the specific surface area (BET) is 45 m2/g.


 1            Hydrophobic nano-TiO2 (Degussa P805) showed high acute mortality in the Pott and Roller (2005)

 2     study. Nano-TiO2 P805 was given at a much lower amount in each instillation than nano-TiO2 P25 and

 3     fine TiO2, because instilled P805 showed acute lethality. A single  intra-tracheal  instillation of P805 at 0.5,

 4     1.0, and 1.5 mg caused death in 25%,  58%, and 92% female Wistar rats, respectively, within 24 hours.

 5     Pott and Roller (2005) originally ordered Degussa T805 for their study, and were unable  to confirm that

 6     the received P805 was the  same as T805.  The physicochemical properties of T805, but not P805, were

 7     used for calculation and reported in the study (Pott and Roller, 2005). In contrast to the high acute

 8     toxicity of hydrophobic nano-TiO2 reported in the Pott and Roller  (2005) study, very low toxicity of

 9     hydrophobic nano-TiO2 was reported in an earlier study by Rehn et al. (2003). Rehn et al. (2003)

10     reported that a single intra-tracheal instillation of P805 at 0.15,  0.3, 0.6, or 1.2 mg caused no death in
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 1    female Wistar rats.  Furthermore, P805 induced only mild, reversible inflammatory responses in the lung,
 2    and was less biologically active than P25 (Rehn et al, 2003).  The reasons for the great discrepancy in the
 3    toxicity of hydrophobic nano-TiO2 (P805 vs. T805 manufactured by Degussa) remain unclear.

 4    5.3.2.2.2. Inhalation
 5          Heinrich et al. (1995) reported increased lung cancer in rats (but not in mice) that inhaled
 6    photocatalytic nano-TiO2. Animals were exposed to P25 aerosols (18 hours/day, 5 days/week) in whole-
 7    body exposure chambers.  Generated by a dry dispersion technique, the nano-TiO2 aerosol had a mass
 8    median aerodynamic diameter of 0.80 (im, with a geometric standard deviation of 1.80.
 9          For female NMRI (Naval Medical Research Institute) mice, the nano-TiO2 exposure was stopped
10    after 13.5 months and followed by clean air exposure for 9.5 months.  The 13.5-month nano-TiO2 aerosol
11    exposure was 4 months at 7.2 mg/m3, 4 months at 14.8 mg/m3, and 5.5 months at 9.4 mg/m3.  Although
12    nano-TiO2 exposures decreased lifespan in mice (50% mortality at 17 months after birth, compared to 20
13    months in controls), the exposures did not increase lung tumor incidence at the end of the study (13.8% in
14    nano-TiO2 exposed, compared to 30% in controls).  Even though the reported spontaneous lung tumor
15    rate seemed to be higher than historical data (20.7% lung cancer in the natural lifespan of female NMRI
16    mice (Lohrke et al., 1984); 12% bronchiole-alveolar lung adenoma and 10% bronchiole-alveolar lung
17    carcinoma in female Han:NMRI mice up to 104 weeks old (Rittinghausen et al., 1997), 13.8% would not
18    be considered as an increase compared to historical controls.
19          For female Wistar rats, the nano-TiO2 exposure was stopped after 24 months, and followed by
20    clean air exposure for 6 months.  The 24-month nano-TiO2 aerosol exposure consisted of 4 months at 7.2
21    mg/m3, 4 months at 14.8 mg/m3, and 16 months at 9.4 mg/m3.  At the end of the 30-month study, 32 of
22    100 nano-TiO2-exposed rats had benign or malignant lung tumors (20 benign squamous cell tumors,  13
23    adenocarcinoma, 4 adenoma,  and 2 squamous cell carcinoma), while only 1 of 217  control rats had lung
24    adenocarcinoma (Heinrich et al., 1995). The lung particle loading was 23.2 mg/lung after 6 months, and
25    39.2 mg/lung after 24 months (Gallagher et al., 1994).  The exposure to nano-TiO2 did not increase the
26    levels of DNA adducts in the lung (Gallagher et al., 1994).  This study showed that  inhaled photocatalytic
27    nano-TiO2 is a lung carcinogen in female rats, but no dose-response relationship can be calculated due to
28    the dosing design. In a parallel study, decreased pulmonary clearance (overload) was clearly
29    demonstrated (Creutzenberg et al., 1990).
30          The aerosol concentrations used in this study, ranging from 7.2 mg/m3 to  14.8 mg/m3, are
31    occupationally relevant, for example, the OSHA PEL (Occupational Safety and Health Administration
32    permissible exposure limit) is 15 mg/m3 and the ACGIH TLV (American Conference of Governmental
33    Industrial Hygienists threshold limit value) is 10 mg/m3.

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 1    5.3.2.2.3. Intraperitoneal Injection
 2          Pott et al. (1987) intraperitoneally injected Wistar and Sprague-Dawley rats with photocatalytic
 3    nano-TiO2 (P25)11 and examined abdominal cavities for tumors.  The treatment doses ranged from a
 4    single intraperitoneal injection of 5 mg nano-TiO2 to 5 injections of 20 mg nano-TiO2 (for a total of 100-
 5    mg nano-TiO2) over 5 weeks (Table 5-9). Tumor incidences were based on rats with sarcoma,
 6    mesothelioma, or carcinoma in the abdominal cavity. Rats with uterine tumors were excluded from the
 7    rats-with-tumor count, because 5-10% of the controls had malignant tumors of the uterus and some with
 8    metastases.  Tumor incidences in the abdominal cavity in nano-TiO2-treated rats ranged from 0% to 10%
 9    in the 5 experiments using nano-TiO2 (Table 5-9). Although controls were not available in all
10    experiments, Pott et al. (1987) concluded there  were no increases in tumor incidence (in the abdominal
11    cavity) in nano-TiO2 treated rats.
       Table 5-9.  Incidence of tumor in the abdominal cavity of rats intraperitoneally injected with
                  photocatalytic nano-TiCh.
Animal, age at the
beginning of the
experiment
Rats sacrificed when
Wistar rat,
9 weeks old
Sprague-Dawley rats,
8 weeks old
Wistar rats,
4 weeks old
Wistar rats,
5 weeks old
Nano-Ti02 treatment
in bad health or 2.5 years after treatment
i.p. injection of 18 mg/rat, once per week for 5 weeks
(totafdose 90 mg/rat)
i.p. injection of 5 mg/rat
i.p. injection of 5 mg/rat
i.p. injections of 2, 4, and 4 mg/rat (total dose 10 mg/rat)
Rats with sarcoma, mesothelioma, or
carcinoma, other than uterine tumors, in the
abdominal cavity (percentage)

6 of 113 rats examined (5.3%)
2 of 52 rats examined (3.8%)
0 of 47 rats examined (0%)
Oof 32 rats examined (0%)
Preliminary results at 28 months after i.p. injection
Wistar rats,
8 weeks old
i.p. injection of 20 mg/rat, once per week for 5 weeks
(total dose 100 mg/rat)
5 of 53 rats (36 rats examined and 17 rats
survived) (9.4%)
       i.p. - intraperitoneal
       Source: Data from Pott et al. (1987).
      1J Data from Pott et al. (1987) reported the P25 as anatase and did not specify particle size in the 1987 publication.
       Currently available P25 is 80% anatase and 20% rutile (primary particle size approximately 21 nm), and a
       representative of Degussa stated that the company has never changed the formula since Degussa P25 was
       introduced to the market (Clancy, pers. comm. 2008).
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 1    5.3.2.2.4. Intramuscular Implantation
 2          No tumors were observed in rats receiving implantations of either conventional TiO2 or nano-TiO2
 3    for up to 12 months (Hansen et al., 2006). Each of the 10 male Sprague-Dawley rats was surgically
 4    implanted with conventional TiO2 (a 9-mm x 2-mm disk containing 100% rutile) subcutaneously on the
 5    left side, and with nano-TiO2 (20-160 nm, mean size 70 nm, 90% anatase and 10% rutile) intramuscularly
 6    on the right side  of paravertebral muscle.  The implanted doses were one disk of conventional TiO2 and
 7    0.1 mL nano-TiO2.  Four rats were sacrificed after 6 months, and the remaining six were sacrificed after
 8    12 months. Inflammation (but not granuloma) was observed at the site of conventional TiO2 implantation,
 9    and granuloma (localized nodular inflammation; non-cancerous inflammation) was observed  at the site of
10    nano-TiO2 implantation at both 6 and 12 months. No tumors were observed at either time.

      5.3.2.3. Modes of Action for Carcinogenicity
11          The mode of action of lung cancer induced by poorly soluble particles with no specific toxicity is
12    believed to be particle deposition in respiratory epithelium, decreased lung clearance (to the degree of
13    overload), persistent inflammation, cellular injury and persistent cell proliferation, fibrosis, and secondary
14    genotoxicity (mutation) in the lung cells (Baan et al., 2006; Muhle and Mangelsdorf, 2003). TiO2 is
15    traditionally considered chemically inert and falls into the category of poorly soluble particles with no
16    specific toxicity. When dose-response is expressed as surface area (dose) to tumor proportion (response),
17    TiO2, nano-TiO2, and other poorly soluble particles with no specific toxicity appear to share the same
18    dose-response curve12 (Dankovic et al., 2007).
19          With the exception of mutation, all the events described in the previous paragraph (Baan et al.,
20    2006; Muhle and Mangelsdorf, 2003) have been reported in rats exposed to both fine TiO2 and
21    photocatalytic nano-TiO2 through inhalation or instillation (Borm et al., 2000; Heinrich et al., 1995; Hext
22    et al., 2002; Pott and Roller, 2005). Figure 5-1 illustrates that, at low or medium exposure levels, lungs
23    with normal clearance show inflammation that diminishes over time after exposure ceases. When the
24    exposure level is high enough to decrease clearance, rats  show persistent pulmonary inflammatory
25    responses (even after exposure ends), cell proliferation and fibrosis, and  eventually tumors. In mice,
26    when the exposure is high enough to cause decreases in clearance, pulmonary inflammatory responses
27    gradually decrease after the exposure ceases and no persistent pathological changes or tumors are
      12 Because the nano-TiO2 data used in this dose-response curve were from studies using the same photocatalytic
       nano-TiO2 product, this dose-response curve might not be applicable to nano-TiO2 with a different crystalline
       type/ratio, purity, shape, surface treatment, or some other characteristic. Although such factors are known to affect
       nano-TiO2 toxicity, their role in carcinogenicity remains unknown.
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 1    observed in the lung. In hamsters, no overload has been observed and therefore no prediction of the
 2    outcome of overload in hamsters is presented here.
 3          Increased mutation frequency in hypoxanthine-guanine phosphoribosyl transferase (hprt) was seen
 4    in type II alveolar cells isolated from rats exposed to 100 mg/kg fine TiO2 through intra-tracheal
 5    instillation (Driscoll et al., 1997). No studies that investigated mutations in lungs of rats exposed to nano-
 6    TiO2 are available. In vitro studies also support the mode of action stated above. Both macrophage- and
 7    neutrophil-enriched BAL cell populations from rats exposed to high concentrations of fine TiO2 showed
 8    increased mutations in cultured cells (rat alveolar type II epithelial cell line; RLE-TN) in vitro (Driscoll et
 9    al., 1997).  Because catalase, an enzyme that catalyzes the decomposition of hydrogen peroxide to water
10    and oxygen, decreased BAL-cell-induced mutation in RLE-TN cells, ROS released from inflammatory
11    cells could contribute to secondary genotoxicity and eventually to the carcinogenicity of TiO2 (Driscoll et
12    al., 1997).  This sequence of events, however, does not appear to occur in mice. At an inhalation dose that
13    causes overload, nano-TiO2 does not appear to increase lung tumors in mice. More specifically, overload
14    occurs in mice at an inhalation concentration of 10 mg/m3 nano-TiO2 (P25), based on the increase of
15    clearance half-life of nano-TiO2 from 40 days at 2 mg/m3 to 395 days at 10 mg/m3, after 13 weeks (6
16    hr/day, 5 days/week) of exposure (Hext et al., 2002). After 13.5 months of inhalation exposure to the
17    same type of nano-TiO2 (P25) at approximately 10 mg/m3 (including 4 months of exposure at 14.8
18    mg/m3), mice showed no increased lung tumors over the 2-year study period (Heinrich et al., 1995).
19          Although the evidence available to date for nano-TiO2 carcinogenesis is consistent with the mode
20    of action of other poorly soluble particles and suggests that particle overload is a sufficient condition for
21    nano-TiO2 to induce lung cancer, this does not definitively establish that particle overload is a necessary
22    condition for nano-TiO2-induced lung cancer. For example, it has been suggested that nano-TiO2-induced
23    lung tumors are directly related to high interstitialization rather than overload (Borm et al., 2000).  Given
24    the paucity of nano-TiO2 cancer studies and the  lack of consensus on exposure-dose metrics, the question
25    arises whether there may be other effects or modes of action unique to nano-TiO2 or nanomaterials in
26    general that are yet to be found.
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                                        TiO9 or nano-TiO9 in air
                                     Deposition in respiratory tract
                          Normal clearance
                   Inflammation diminishing over
                        time post-exposure
     Decreased clearance (overload)
                                                                         Rats
        Persistent inflammation
                                                          Cellular injury, persistent cell
                                                              proliferation, fibrosis
                                                                    Mutation
                                                                  Lung tumors
      Figure 5-1.     The pulmonary effects of fine TiCh and nano-TiCh exposure through inhalation or
                     instillation.
 1          Although the carcinogenicity of TiO2 and nano-TiO2 in rats at high doses has been shown
 2    repeatedly in inhalation and instillation studies, the relevance of this rat-specific response to human health
 3    is under debate. Rats have been suspected to be more sensitive to poorly soluble particle-induced lung
 4    cancer because they are more prone to pulmonary inflammation (Muhle and Mangelsdorf, 2003).
 5    Furthermore, lung tumors induced by poorly soluble low-toxicity particles are limited to rats with
 6    severely compromised particle clearance in lung (overload) (Hext et al., 2005).  In human exposures,
 7    people working in dusty environments, such as coal miners, could encounter high concentrations of
 8    particles and have impaired lung clearance (Baan et al., 2006). Coal miners, however, are likely to be
 9    exposed to a mixture of particles (i.e., not limited to poorly soluble low-toxicity particles).  Evidence of
10    persistent or chronic inflammation in humans exposed to TiO2 is suggested only by case studies of
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 1    workers exposed to TiO2 and other minerals (Keller et al., 1995; Moran et al., 1991; Yamadori et al.,
 2    1986).

      5.3.2.4. Summary of Carcinogenic Effects
 3          The results of nano-TiO2 carcinogenicity studies in animals are summarized in Table 5-10. No data
 4    are available for nano-TiO2 carcinogenicity in humans or for photostable nano-TiO2 in animals.  TiO2 (not
 5    specific to nano-TiO2) was classified as "possibly carcinogenic to humans" (Group 2B) by an IARC
 6    Monographs Work Group in 2006 (Baan, 2007), and "carcinogenic" (Class D2A) by WHMIS (CCOHS,
 7    2006). NIOSH (2005) proposed not designating TiO2 as a "potential occupational carcinogen" because of
 8    insufficient evidence, but expressed concern about the potential carcinogenicity of ultrafine TiO2 (nano-
 9    TiO2) at the  current exposure limits.  Based on calculated lung cancer risks, the draft NIOSH
10    recommendation was an exposure limit of 0.1 mg/m3 for ultrafine TiO2 and 1.5 mg/m3 for fine TiO2 (less
11    than 2.5 (im), as time-weighted average concentrations. The relevance of rat-specific nano-TiO2
12    carcinogenicity to human health remains to be elucidated.
       Table 5-10.   Results of nano-TiCh carcinogenicity studies in animals.
Exposure route Species
Result
Lowest effective dose
(highest ineffective dose)
References
Photocatalytic nano-TiCh
Intra-tracheal Wistar rats,
instillation female
Wistar rats,
female
Inhalation
NMRI mice,
female
Intranpritnnpql Wistar and
Infraoentoneal ^ague.Dawley
Increased lung tumors
(benign and malignant)
Increased lung tumors
No increases in lung tumors
No increase in abdominal
tumors
5 instillations at 6.0 mg/instillation
5 instillations at 3.0 mg/instillation
Approximately 1 2 mg/m3 for 24 months a
(Approximately 10 mg/m3 for 13.5
months) b
(5 intraperitoneal injections at 18 mg/rat
per injection)
Bormetal. (2000)
Pott and Roller
(2005)
Heinrich etal.
(1995)
Heinrich etal.
(1995)
Pott etal. (1987)
Nano-TiCh with unspecified photoreactivity c
Intra-tracheal Wistar rats,
instillation female
Intramuscular Sprague-Dawley
implantation rats, male
No conclusion d
No increases in tumor at
implantation sites
(30 instillations at 0.5 mg/instillation)
(not specified)
Pott and Roller
(2005)
Hansen et al.
(2006)
       NMRI = Naval Medical Research Institute
       a 7.2 mg/m3 for 4 months, followed by 14.8 mg/m3 for 4 months and then 9.4 mg/m3 for 16 months
       b 7.2 mg/m3 for 4 months, followed by 14.8 mg/m3 for 4 months and then 9.4 mg/m3 for 5.5 months
       c Nano-Ti02 particles not specified or have questionable identification
       d Unexpected high acute toxicity; problem with ascertaining the identity of testing material
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                         Questions  about Health  Effects
General

 5.3-1.  Are the current EPA harmonized health test guidelines for assessing toxicity adequate to determine the health
        effects/toxicity of nano-Ti02?

Dermal toxicity

 5.3-2.  Is the current information on nano-Ti02 skin penetration sufficient for risk assessment?

 5.3-3.  Would nano-Ti02 penetrate into living cells in flexed, "soaked," or damaged skin (such as sunburned, scratched,
        eczematous skin)?

 5.3.4.  How important is testing nano-Ti02 skin penetration on different races and at different ages?

 5.3.5.  Do certain formulations of nano-Ti02 sunscreens generate hydroxyl radicals when applied to skin?

 5.3-6.  Given that nano-Ti02 is a good antimicrobial agent, how does it affect skin flora? Does application of sunscreen
        promote the colonization of skin by potentially harmful bacteria (e.g., staph)?

 5.3-7.  To what extent do photocatalytic properties of nano-Ti02 contribute to dermal effects?

Respiratory toxicity

 5.3-8.  What kind of studies would provide the most suitable data to understand dose-response of nano-Ti02 occupational
        exposure and health effects  in humans?

Reproductive toxicity

 5.3-9.  What is the potential for reproductive and developmental effects of nano-Ti02?

Carcinogenicity

5.3-10.  Is ingested nano-Ti02 carcinogenic?

5.3-11.  Is inhaled nano-Ti02 carcinogenic at exposure levels below those that induce particle overload?
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           Appendix A.  Nano-TiOz  in  Sunscreen:

                        Background  Information

 1         Nanoscale titanium dioxide (nano-TiO2) has been used in topical sunscreen products since around
 2    1990 (Environmental Working Group, 2008). Between 1995 and 2002, the market for inorganic
 3    sunscreen ingredients (both nanoscale and non-nanoscale) increased from a value of roughly $30 million
 4    to a value of about $38 million, and has maintained about a 20% share of the sunscreen ingredient market
 5    as a whole (Dransfield, 2005). Dransfield (2005) projected that the inorganic active sunscreen ingredient
 6    market would grow to approximately $75 million by 2010, and that inorganic active ingredients would
 7    account for one-third of the total active sunscreen ingredient market. Dransfield (2005) suggested that the
 8    projected increase in the popularity of inorganics can be attributed to improved transparency in the
 9    products, which would imply particularly rapid growth in the market for nanoscale inorganics. In 2006,
10    the Australian Therapeutic Goods Administration (TGA) estimated that 70% of titanium sunscreens and
11    30% of zinc sunscreens in Australia were formulated with nanoparticles (TGA, 2006).
12         The U.S. topical sunscreen market in 2000 was  approximately $553 million (65%) of the $853
13    million "sun-care" market (a category that includes self-tanning products, after-sun products, etc.)
14    (Packaged Facts, 2001).  The size of the  U.S. sunscreen market had apparently not changed  substantially
15    since 1993, when retail sales were reportedly in the range of $550-575 million (Davis, 1994).  The total
16    U.S. sun-care market reached $1.1 billion in 2005, and is projected to reach $1.2 billion by 2010 (Jeffries,
17    2007). If sunscreens continue to account for 65% of the U.S. sun-care market, that would translate to
18    $715 million in sunscreen sales in 2005, and a projected $780 million in sunscreen sales in 2010.
19    Globally, sales of sun protection products that presumably include topical sunscreens and cosmeceuticals
20    were expected to exceed $820 million in 2006 (Newman,  2006). As a "mature" market in the United
21    States, sun protection products are expected to have a  growth rate of only about 2% per year (Jeffries,
22    2007). Between 2005 and 2010, however, growth in the sun-care market was expected to be much faster
23    abroad than in the United States (Jeffries, 2007). If the growth in cosmeceuticals has dampened demand
24    for conventional sunscreen, this growth has led to even greater demand for active sunscreen ingredients,
25    including micronized TiO2 (Davis, 1994).
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      A.1.  Sunscreen Chemistry, and the  Role and Properties of
             Nano-TiOz

 1         Ultraviolet (UV) radiation is classified by wavelength into three types: UV-A (320-400
 2    nanometers [nm]), UV-B (290-320 nm), and UV-C (200-290 nm). The shorter the wavelength, the more
 3    energy the UV radiation transmits. Consequently, the shorter wavelength rays can cause more damage to
 4    skin than the longer wavelength rays. About 10% of the solar radiation that reaches Earth's surface is UV,
 5    and about 95% of that is UV-A. The long wavelengths of UV-A contribute to skin aging, skin wrinkling,
 6    and skin cancer.  UV-B is in the middle range of UV, and contributes to burning and tanning, skin aging,
 7    and skin cancer.  Although UV-C has the shortest wavelength and can be dangerous, it is blocked by
 8    ozone in the atmosphere and does not reach Earth's surface (Jeffries, 2007; Shao and Schlossman, 1999).
 9         The traditional sunburn protection factor (SPF) rating system measures protection against UV-B
10    radiation only. The Food and Drug Administration (FDA) proposed an official rating system that also
11    takes UV-A radiation into account, awarding sunscreens between one and four stars based on their UV-A
12    protection.  This  system was expected to go into effect in November 2008 or later (72 FR 49070).
13    Various other UV-A protection ratings systems are in use or have been proposed in Australia, New
14    Zealand, Europe, Japan, China, and Korea (Moyal, 2008).

      A.1.1. Size of Nano-TiCh  Particles (Mean and Distribution)

15         The composition of nano-TiO2-based sunscreens is determined or constrained by several factors,
16    including peculiar properties of nano-TiO2, general principles  of sunscreen chemistry, and aesthetic and
17    other concerns. The size of nano-TiO2 particles (both the primary particle size and  the effective particle
18    size of aggregates and agglomerates) affects protection against UV-A and UV-B radiation, the opacity of
19    the sunscreen, and the stability of the dispersions.  In most cases, a range of nano-TiO2 sizes is present
20    due to various primary particle sizes and aggregation.
21         The size of nano-TiO2 particles affects how much UV-A and UV-B the particles transmit and
22    scatter, and therefore, the degree of protection the particles provide against UV-A and UV-B radiation.
23    Shao and Schlossman (1999) found that a nano-TiO2 dispersion with a primary particle size of about
24    15 nm transmitted less UV-B and more UV-A and visible light than did dispersions with primary particle
25    sizes of 35, 100,  and 200 nm. (The particles were present in aggregates of mean sizes 125.3, 154.1,
26    251.1, and 263.4  nm, respectively.) The results of this study indicate that smaller nano-TiO2 particles are
27    better for UV-B protection, and larger nano-TiO2 particles are  better for UV-A protection.  Dransfield
28    (2005) presented data indicating that TiO2 particles (not specifying whether they were primary or
29    secondary particles) in the range of 40-100 nm provide the best UV-A protection, and particles in the
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 1    range of 60-220 nm provided the best UV-B protection. According to Hewitt (2002), theoretical
 2    calculations suggest that the optimal mean TiO2 primary particle size for good UV-B and UV-A protection
 3    is about 50 nm. Chaudhuri and Majewski (1998) noted that nano-TiO2 with a primary crystal size of 10-
 4    20 nm and an effective particle size of about 100 nm is expected to have a "very high UV scattering
 5    effect."
 6          Particle size also determines the opacity of nano-TiO2 formulations.  Larger primary particles
 7    transmit less visible  light (Shao and Schlossman,  1999). Aggregation will also make a formulation more
 8    opaque (Chaudhuri and Majewski, 1998).  TiO2 particles larger than 200 nm in sunscreen or cosmetics
 9    leave a white hue on the skin and are considered aesthetically unacceptable in many applications.  Nano-
10    TiO2 particles smaller than 100 nm are generally not visible, and the sunscreen appears transparent when
11    applied. A presentation by Schlossman et al. (2006) included pictures demonstrating the opacity of
12    formulations with different particle sizes when applied to skin. Formulations with an effective
13    agglomerated particle size of 100-120 nm  (primary particle size of 10 nm) or 120-150 nm (primary
14    particle size of 15 nm) were transparent or nearly transparent.  Schlossman et al. (2006) noted that, in
15    addition to particle size, two other factors affected the opacity/transparency of formulations: the
16    difference between the refractive index of the particle and that of the media, and the uniformity of particle
17    dispersion.
18          Chaudhuri and Majewski (1998) noted that particle size also affects the stability of sunscreen
19    dispersion. The reason for this was not made clear in the article, but in a discussion of pigmentary
20    particles in paints, Himics and Pineiro (2008) explained that smaller pigmentary particles produce a better
21    dispersion because the larger surface area creates a higher viscosity, which prevents settling and
22    clumping. The phenomenon that Chaudhuri and Majewski (1998) noted could have a similar explanation.
23          A range of particle sizes provides a range of UV protection, but too wide a range could pose a risk
24    of opacity or of compromising the stability of the dispersion (e.g., if too many particles are too large). In
25    the past, controlling  the range of particle sizes produced by manufacturing processes was difficult, and
26    distributions with a mean particle size of 50 nm included particles in the visible range. As technology has
27    improved, creating particles of desired size and size distributions with much greater accuracy (Hewitt,
28    2002) has become possible.
      A.1.2. Active Ingredient Purity
29          The U.S. Pharmacopeia (USP) sets reference standards for TiO2 and other active ingredients in
30    over-the-counter and prescription drugs. The 2006 edition of the USP national formulary monographs,
31    USP-NF 30 (U.S. Pharmacopeia, 2006), declares that TiO2 "contains not less than 99.0% and not more
32    than 100.5 percent of TiO2." For "attenuation grade" TiO2, that determination is made on an ignited basis.
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 1    USP specifies tests for water-soluble impurities, acid-soluble impurities, arsenic, and organic volatile
 2    impurities, and notes that FDA also has set limits on acceptable lead, antimony, and mercury
 3    contamination. USP also specifies that the material must be stored in well-closed containers, and that it
 4    be properly labeled as attenuation grade (with names and amounts of added coatings, stabilizers, and
 5    other treatments listed) if intended for UV-attenuation.
      A.1.3. Photostability and Surface Coating/Doping
 6          Nano-TiO2 is a natural semiconductor with photocatalytic properties. Its electrons can easily
 7    become excited by energy absorbed from UV radiation. When the electrons return to ground state, longer
 8    wavelength radiation is emitted. Alternatively, if the energized electrons escape from the particle, they
 9    can catalyze chemical reactions (oxidation/reduction processes) in nearby molecules. These reactions can
10    create free radicals, which can damage skin cells or degrade other sunscreen ingredients. The choice of
11    nano-TiO2 crystal affects photostability.  In particular, rutile is much more photostable than anatase
12    (Chaudhuri and Majewski, 1998; Maynard, 2008).  Although anatase is less photostable, it appears to be
13    in common use. Barker and Branch (Barker and Branch, 2008) studied five TiO2 sunscreens purchased
14    over the counter and found that one was pure rutile and the other four were anatase/rutile mixes in which
15    anatase predominated.
16          To increase TiO2 and nano-TiO2 photostability (i.e., to reduce the likelihood that excited electrons
17    will escape), the crystals are commonly given a surface coating.  Coating TiO2 with silicon dioxide and
18    alumina (3.5% by weight) can reduce photocatalytic activity by 99% (SCCNFP, 2000).  Other TiO2 or
19    nano-TiO2 surface coatings mentioned in the literature include inorganic oxides (Bird, 2002), simethicone
20    (Chaudhuri and Majewski, 1998), methicone, lecithin (Schlossman et al., 2006), stearic acid, glycerol,
21    silica, aluminum stearate, dimethicone (SCCNFP, 2000), metal soap, isopropyl titanium triisostearate
22    (ITT), triethoxy caprylylsilane, and C9-15 fluoroalcohol phosphate (Shao and Schlossman, 1999).
23    Alumina is often used in combination with other coating materials. The amount of surface coating
24    applied varies substantially from product to product.  For  examples of common coating concentrations
25    and combinations, see Appendix B, Table B-2.
26          Another technique for increasing photostability is "doping" the TiO2 or nano-TiO2 particles by
27    embedding within them minute amounts  of metals such as manganese, vanadium, chromium, and iron
28    (Park et al., 2006).  Doping rutile nano-TiO2 with manganese is reported to increase UV-A absorption,
29    reduce free radical generation, and increase free radical scavenging behavior (Reisch, 2005; Wakefield et
30    al., 2004). Doped TiO2 is colored instead of white, which can have desirable cosmetic effects in products
31    such as skin lighteners (Park et al., 2006).
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 1          Recent research by Barker and Branch (2008) has found that the surface coatings on nano-TiO2 in
 2    many sunscreens might not be stable or effective. The investigators studied the weathering of paint in
 3    contact with sunscreen. Out of five nano-TiO2 sunscreens tested, four released photocatalytically
 4    generated hydroxyl radicals that accelerated the weathering of the paint. All four of those sunscreens
 5    used an anatase/rutile mix.  The one nano-TiO2 sunscreen that showed no appreciable effect on paint
 6    weathering was Oxonica's Optisol, which is 100-percent rutile, and is doped with manganese rather than
 7    surface-coated. It is not know whether nano-TiO2 sunscreens generate hydroxyl radicals when applied to
 8    skin or whether such hydroxyl radicals would penetrate the skin and pose a threat to the health of the
 9    sunscreen user (Brausch and Smith, 2009; Maynard, 2008).
      A.1.4.  Dispersion and pH Considerations
10          Nano-TiO2 can exist as a dry powder, but most sunscreen applications require the particles to be
11    suspended in a fluid medium. This liquid is called a "dispersion" because special care must be taken to
12    ensure that nano-TiO2 will be distributed evenly and to minimize further aggregation and agglomeration
13    (which could negatively impact UV scattering performance, transparency, etc., by increasing the effective
14    particle size).  Sunscreen manufacturers can purchase nano-TiO2 powder and formulate their own
15    dispersion, or they can purchase ready-made "predispersions."
16          In an effective dispersion, suspended particles are attracted to the dispersion medium and repel
17    each other. Surface coatings influence the interaction of nano-TiO2 with the dispersion medium, which
18    can be water-based (aqueous), oil-based, or silicone-based.  Early TiO2 dispersions were generally oil-
19    based (Bird, 2002). Surface coatings that make TiO2 dispersible in non-aqueous media can be lipophilic
20    (e.g., metal soap, ITT, lecithin); hydrophobic (e.g., methicone, dimethicone, triethoxy caprylylsilane); or
21    both (e.g., C9-15 fluoroalcohol phosphate) (Shao and Schlossman, 1999).  For methicone and C9-15
22    fluoroalcohol phosphate, silicone might be the preferred medium (Shao and Schlossman, 1999).  Bird
23    (2002) states that coatings have been developed to enable TiO2 to be dispersed effectively in aqueous
24    media as well, but provides no examples.  Chaudhuri and Majewski (1998) describe one product, an
25    "amphiphilic" powder (Eusolex® T-2000) containing about 80-percent USP-grade rutile coated with
26    alumina and simethicone, that is easily dispersible in both water and oil.
27          Two related concepts that are useful in discussing the dispersion of particles  are the pH at the point
28    of zero charge (pHpzc), which is the point at which the surface charge density of a particle is zero, and the
29    isoelectric point (IEP), which is the pH at which the net surface  electric charge of a particle is zero.  In
30    situations where no ions other than FT and OH- are adsorbed at the particle surface, pHpzp is identical to
31    the IEP.
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 1          At most pH values, nano-TiO2 particles suspended in a dispersion have a positive electrical charge
 2    or a negative electrical charge and repel each other.  At the pHpzc/IEP, however, there is no electrostatic
 3    repulsion, and particles tend to agglomerate (Hewitt, 1995).  To maintain electrostatic repulsion and
 4    prevent agglomeration, the dispersed product must be maintained at a pH other than the IEP (usually at a
 5    lower pH) at every stage of production and storage.
 6          Surface coating can affect a particle's pHpzc/IEP and can potentially extend the pH range at which
 7    the dispersion can be handled. For example, uncoated nano-TiO2 has an IEP of pH 6, and nano-TiO2
 8    coated with alumina and simethicone has an IEP of pH 9 (Chaudhuri and Majewski, 1998).  Bird (2002)
 9    cites lecithin as another coating that is advantageous for electrostatic reasons.
10          Experimental tests show additional pH considerations. Nano-TiO2 performance can be adversely
11    affected by strongly acidic formulations (effects include more agglomeration, lower SPF, and greater
12    opacity), unless special formulating techniques are used (Hewitt, 1995).
13          Additional compounds can be added to the dispersion as "dispersants." "[The] proper dispersant
14    can help particles to disperse into [the] vehicle so as to shorten the dispersion time and increase the degree
15    of dispersion. It can reduce the viscosity and yet stabilize the dispersion by either electrostatic or steric
16    repellency" (Shao and Schlossman, 1999). Different dispersants are used in water- and oil- (or silicone-)
17    based formulations. PEG-10 dimethicone is used as a dispersant for nano-TiO2 in a cyclopentasiloxane
18    carrier in the predispersion CM3K25VM Kobo Products, Inc. manufactures. Polyhydroxystearic acid is
19    used as a dispersant in a C12-15 alkyl benzoate carrier in Kobe's TNP40TPPS predispersion (Shao and
20    Schlossman, 2004). Mitchnick  and O'Lenick (1996) mention lecithin and phosphate esters as potential
21    "dispersing aids" for TiO2 dispersions, but they also use language suggesting that they might actually
22    mean surface coatings.
      A.1.5.  Distribution of Active Ingredient in Emulsion
23          Most sunscreens are emulsions - mixtures of two fluids (called "phases") that are immiscible (do
24    not combine easily). For instance, water and oil, two immiscible fluids, may be mixed in an emulsion by
25    an energetic process such as stirring or shaking.  In some cases, the two fluids tend to quickly separate
26    again. To prevent separation, an emulsifier (typically a surfactant or a polymer) can be added. In an
27    emulsion containing two types of liquids, generally, droplets of one fluid are dispersed in a larger amount
28    of the other fluid.  The two fluids are referred to as the "dispersed phase" and the "continuous phase,"
29    respectively.
30          Types of emulsion used in sunscreens and other cosmetic  products include oil in water (in which an
31    oil phase is dispersed in a water phase, abbreviated "o/w"); water in oil (w/o); water in water (w/w); and
32    occasionally water in oil in water (w/o/w).  In "oil-free" formulations, oil is substituted by silicones
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 1    (w/Si, Si/w) (Hewitt, 2000). As noted above, nano-TiO2 is most easily dispersed in oil, but emulsions can
 2    be formulated with nano-TiO2 in a water phase, an oil phase, or a silicone phase. The nano-TiO2 can be
 3    present in the dispersed phase or the continuous phase of a sunscreen emulsion (Dransfield, 2005).
 4          The emulsifiers used to keep the two phases from separating are typically partially hydrophilic and
 5    partially hydrophobic (or even lipophilic). By gathering on the interface between the dispersed phase and
 6    the continuous phase, emulsifiers bind the two phases (this is the principle behind soaps, shampoos, and
 7    detergents, which enable water to wash away oils and other normally hydrophobic particles), or at least
 8    prevent the two phases from repelling each other. Emulsifiers used in sunscreen emulsions include
 9    glyceryl stearate, PEG-100 stearate, and polyglyceryl-3-methyl glucose distearate (Oxonica, 2005).
      A.1.6. Other Ingredients, Active and  Inactive
10          Nano-TiO2 can be combined with other physical UV blockers, such as zinc oxide (ZnO) (which can
11    also be micronized), or with chemical UV filters to improve the UV protection the sunscreen provides.
12    The sunscreen formula can also include a diverse array of inactive compounds for a variety of purposes.
13          TiO2 and ZnO can form agglomerates. This attribute presents an obstacle to using TiO2 and ZnO in
14    the same sunscreen.  A solution is to put one active ingredient in the oil phase  of the emulsion and the
15    other in the water phase (Hewitt, 1995).
16          Combining nano-TiO2 with chemical UV filters often provides better UV-B protection than
17    expected, based on the SPF of each ingredient. The improved protection is probably due to the scattering
18    the physical UV blocker provides, which increases the optical path length of the radiation and creates
19    more opportunities for absorption by the chemical filter (Bird, 2002; Chaudhuri  and Majewski, 1998).
20          Emollients are often included in sunscreens to make the products feel more pleasing on the skin or
21    to moisturize. In excessive quantities, emollients could break down the dispersion microstructure. This
22    effect can be counteracted by using suitable surfactants or polymers (Hewitt, 1996).
23          Increasingly, nano-TiO2 is found in "cosmeceuticals," products that combine a variety of active
24    ingredients to perform multiple  health and beauty functions.  These products include moisturizers and
25    color cosmetics (see below for more on cosmeceuticals). The manganese added to some nano-TiO2
26    formulations to prevent formation office radicals during UV exposure can also help scavenge free
27    radicals generated by other means, thus providing extra skin-protection benefits.
28          Inert ingredients can be added to achieve the right viscosity or liquidity, spray-ability, color or
29    transparency, pH, water-resistance, or spreadability. Silicones and related compounds can be added to
30    impart water-resistance, improve skin feel, serve as emulsifiers in various formulations, and enhance the
31    SPF of oil-based dispersions (Hewitt, 2000).
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    A.2. Some Sunscreens with Nano-TiOz or Micronized
          as Active Ingredient

1        Table A-l was compiled in 2007 from information contained in the Environmental Working
2   Group's cosmetic database "Skin Deep" (Environmental Working Group, 2008) and from on-line
3   shopping sources.  Products labeled as containing TiO2 of unspecified particle size were excluded. The
4   list of products provided in Table A-l is likely not exhaustive. Also, product formulations and labels
5   could change over time.
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Table A-1.     Titanium dioxide (TiCk) content in various sunscreen products.
Brand/ Manufacturer
Abella
Alba Botanica
B. Kamins
BABOR
BABOR
BENEV
Bliss
California Baby
California Baby
California Baby
Cellex-C
Cellex-C
Cellex-C
Cellex-C
Colorescience
Dermalogica
Dermalogica
EmerginC
Fallene/Total Block
Fallene/Total Block
Fallene/Total Block
Fallene/Total Block
Jan Marini
June Jacobs
Lancome
Lancome
Peter Thomas Roth
Pevonia Botanica
ProCyte
ProCyte
ProCyte
ProCyte
ProCyte
Total Skin Care LLC
Wilma Schumann
Product
Solar Shade, SPF 45
Chemical Free Sunscreen, SPF 18
Chemist Bio-Maple Sunbar Sunscreen, SPF 30 Fragrance-Free
High Protection Lotion, SPF 30
Moderate Protection Sun Cream, SPF 20
Pure Ti02
Oil-free Sunban Lotion for the Face, SPF 30
SPF 30 & Fragrance Free Sunscreen; also available as Sunblock Stick, SPF 30
Sunscreen SPF 30+ - Everyday Year Round; also available as Sunblock Stick
Water Resistant, Hypo-Allergenic Sunscreen, SPF 30
Sunscreen, SPF 15
Water Resistant Sunscreen, SPF 30
Sun Care Broad Spectrum UV-A, UV-B Sunblock & Moisturizer, SPF 15
Sun Care, SPF 30
SPF 30 All Clear Sparkles Shaker Jar; SPF 30 Perfectly Clear Sparkles Shaker Jar; SPF 30
Almost Clear Sparkles Shaker Jar; these variations also available in trial size, brushable, and
rock and roller ball forms
Oil Free Matte Block, SPF 20
Ultra Sensitive FaceBlock, SPF 25
Sun 30 (and tinted version)
Total Block Clear, SPF 65
CoTZ, SPF 58
Total Block Cover-Up/Make-Up, SPF 60
Total Block Tinted, SPF 60
Bioglycolic Facial Lotion, SPF 15
Micronized Sheer, SPF 30
Soleil High Protection Face Cream - Gel, SPF 30
Soleil Soft-Touch Moisturizing Sun Lotion, SPF 15
Instant Mineral, SPF 30
Pevonia Soleil Sun Block, SPF 15
Ti-Silc Sheer, SPF 45
Ti-Silc Sheer, SPF 45 (tinted)
Ti-Silc Sunblock, SPF 60+
Ti-Silc Untinted, SPF 45
Z-Silc Plus Sunblock, SPF 30+
pH Advantage Basics Sun Blocker, SPF 15
Wilma Schumann Sunscreen, SPF 20
Percentage TiCh
N/A
7.0%
2.04%
N/A
4.5%
N/A
6%
4.5%
4.5%
N/A
2%
2%
N/A
2%
12%
4%
14%
N/A
4%
10%
10%
10%
5.5%
14.5%
4.5%
4.5%
15%
N/A
N/A
3.5%
8%
3.5%
4.0%
N/A
N/A
N/A - Not available.
Source:  Skin Deep Database (Environmental Working Group, 2008).
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A.3. References
Barker PJ; Branch A. (2008). The interaction of modern sunscreen formulations with surface coatings.
       Progress in Organic Coatings 62: 313-320.
Bird S. (2002, March). Sense and stability. Soap Perfum Cosmet, 75, 42-44.
Brausch JM; Smith PN. (2009). Pesticide resistance from historical agricultural chemical exposure in
       Thamnocephalus platyurus (Crustacea: Anostraca). Environmental Pollution 157: 481-487.
Chaudhuri RK; Majewski G. (1998). Amphiphilic microfine titanium dioxide: Its properties and
       application in sunscreen formulations. Drug Cosmet Ind 162: 24-31.
Davis DA. (1994). Sunscreen oddities. Drug Cosmet Ind 155: 20-24.
Dransfield G. (2005). Manufacture of novel, transparent TiO2 based sunscreens.  Retrieved May 9, 2008,
       from http://www.wun.ac.uk/nanomanufacturing/archive/05_06_series/documents/dransfield.pdf.
Environmental Working Group. (2008). Sunscreen Investigation: Skin Deep-Cosmetic Safety Reviews.
       Retrieved June 2, 2009, from http://www.cosmeticsdatabase.com/special/sunscreens2008/.
Hewitt JP. (1995). Formulating with physical sunscreens: Control of emulsion pH. Drug Cosmet Ind 157:
       28-32.
Hewitt JP. (1996). The influence of emollients on dispersion of physical sunscreens. Drug Cosmet Ind
       159: 62-65.
Hewitt JP. (2000). Partners in protection. Soap Perfum Cosmet 73: 85-86.
Hewitt JP. (2002). A moment of clarity. Soap Perfum Cosmet 75: 47-50.
Himics R; Pineiro R. (2008). The importance of particle size in liquid coatings. Products Finishing
       Magazine from Gardner Publications, Inc.
Jeffries N. (2007). SPF, efficacy and innovation. Global Cosmetics Industry (GCI) Online Magazine -
       February 2007 Issue.  Retrieved May 2, 2008, from
       http://www.gcimagazine.com/marketstrends/segments/suncare/27627099.html.
Maynard AD. (2008). Living with nanoparticles. Nano Today 3: 64-64.
Mitchnick M; O'Lenick AJ, Jr.  (1996). U.S. Patent #5565591, class: 556/10. Silicone polymers for the
       modification of titanium dioxide.
Moyal D. (2008). How to measure UVA protection afforded by sunscreen products. Expert Review of
       Dermatology 3: 307-313.
Newman KA. (2006, December 5, 2006). Sun protection report. Global Cosmetic Industry (GCI)
       Magazine - December  2006 Issue, from
       http://www.gcimagazine.com/marketstrends/segments/suncare/4829426.html ?page=l.
Oxonica. (2005). Technical Notes: OptisolTM UV Absorber Regulatory Status.  Retrieved September 18,
       2007, from http://www.oxonica.com/_get_file.php?file=22_l_technote-
       regtox.pdf&cat=promo_lit.
Packaged Facts. (2001, March, 2001). The U.S. Market for Suncare and Lipcare Products. Retrieved
       June 2, 2009, from http://www.mindbranch.com/listing/product/R567-393.html.
Park GB; Knowland JS; Flutter BR. (2006). U.S.  Patent #20060134026, class: 424/59. Sunscreens.
Reisch M. (2005). New-wave sunscreens. Chemical and Engineering News 83: 18-22.
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SCCNFP. (2000). Opinion of the scientific committee on cosmetic products and non-food products
       intended for consumers concerning titanium dioxide. Brussels, Belgium.
Schlossman D; Shao Y; Detrieu P. (2006, October 10, 2006). Perspectives on supplying attenuation
       grades of titanium dioxide and zinc oxide for sunscreen applications. Public meeting on
       nanotechnology materials in FDA regulated products  Retrieved October 28, 2007, from
       http://www.fda.gov/nanotechnology/meetings/kobo_files/textmostly/index.html.
Shao Y; Schlossman D. (1999). Effect of particle size on performance of physical sunscreen formulas.
       from http://www.koboproductsinc.com/Downloads/PCIA99-Sunscreen.pdf.
Shao Y; Schlossman D. (2004). Discovering an optimum small micropigment for high UV shielding and
       low skin whitening. Retrieved April 4, 2008, from
       http://www.koboproductsinc.com/Downloads/IFSCC2004.pdf
TGA. (2006). A review of the scientific literature on the safety of nanoparticulate titanium dioxide or zinc
       oxide in sunscreens.  Retrieved September 24, 2008, from
       http://www.tga.gov.au/npmeds/sunscreen-zotd.pdf
U.S. Pharmacopeia. (2006). Titanium Dioxide. In U.S. Pharmacopeia Official Monographs (Vol. 30, pp.
       3364).

Wakefield G; Lipscomb S; Holland E; Knowland J. (2004). The effects of manganese doping on UVA
       absorption and free radical generation of micronised titanium dioxide and its consequences for
       the photostability of UVA absorbing organic sunscreen components. Photochem Photobiol Sci 3:
       648-652.
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       Appendix B. Nano-TiOz in  Sunscreen

               Manufacturing Processes


   B.1. Overview of Nano-Ti02 Manufacturing Process

1      A generic manufacturing process for nano-TiO2 for sunscreen applications is outlined in
2  Figure B-l.
                            Nuclei Synthesis

                               Drying
                          Dispersion / Milling
                                  Source: Reprinted with permission from Dransfield (2005).


           Figure B-1.   Generic manufacturing process for nano-TiCk for
                    sunscreens.
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      B.1.1.  Titanium  Dioxide Nuclei Synthesis
 1          Commercial-scale TiO2 synthesis is mostly by sulfate or chloride processes. In this section, a
 2    sulfate process, chloride process, and patented Altair process are described. These three processes can be
 3    used to synthesize both conventional (or pigmentary) and nanoscale TiO2.  There are many new processes
 4    being developed in the laboratory, but it is outside the scope of this Appendix to cover them (see review
 5    of nano-TiO2 synthesis by (Chen and Mao, 2007). The sulfate process and the chloride process,
 6    illustrated in Figure B-2, are two common methods used to produce TiO2 in a variety of grades for many
 7    different applications.
                                       TiO2 Processes Compared
                                Sulfate Process
                                Hydrolysis, Filtration
                                   & Washing
_ Dilute
* H,SO,
                                    Calcination
              Chloride Process
                           [Chlorine
                                                          Natural or Synthetic Rutile
                                                           Coke
                                                                    Chlori nation
                                                                    Purification
                                                                               Impurities
Pure TiCI4
                                                                     Oxidation
                             Milling & packing
                          of untreated TiO2 pigment
                                               "•*] Milling, Classification |
                                                Drying, milling & packing
                                              of surface treated TIO2 Pigment
                                        Source: Reprinted with permission from Millennium Inorganic Chemicals (2007).
                       Figure B-2.   Sulfate and chloride processes for TiCfc manufacture.
 8          The sulfate process, a wet process for creating pigmentary TiO2, dates from around 1930, and it
 9    was the dominant method used to produce TiO2 until the chloride process was developed in the 1950s
10    (Hext et al., 2005). The chloride process now accounts for about 60% of worldwide TiO2 pigment
11    production (Hext et al., 2005).  The chloride process, a gas-phase process, is more energy efficient than
12    the wet-phase sulfate process; it can produce finer particles and particles with  specific morphologies
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 1    (Osterwalder et al., 2006). The sulfate process is used primarily to create pigmentary particles. Because
 2    attenuation-grade TiO2 can be produced using "the same processes as larger pigmentary grades":
 3    (Schlossman et al., 2006), the sulfate process and the chloride process are considered in this document as
 4    possible manufacturing techniques for nano-TiO2 in sunscreen.
 5          The sulfate process and the chloride process differ in the feedstock and techniques for nuclei
 6    synthesis.  In both processes, particles are milled and surface-treated to prepare them for the intended
 7    application. The "surface treatment" step in Figure B-2 corresponds to the "coating" step in Figure B-l.
 8          The Altair process, a patented, spray-hydrolysis-based process, is illustrated in Figure B-3.  This
 9    process is used by Altair Nanotechnologies, Inc. to produce not only coated nano-TiO2 for sunscreen
10    applications, but also uncoated and larger TiO2 particles and several ceramic oxides (Verhulst et al.,
11    2003). The feedstock for this process is titanium oxychloride.  This patented process is comparable in
12    many respects to the sulfate process. What makes it unique, according to Verhulst et al. (2003), is the
13    spray hydrolysis step, which eliminates the aqueous filtration step.
                                Feedstock

                              Additives	

                              Control of
                              flow rates,
                             temperature,
                             drop size etc
                             Additives 	
                             Additives
    Solution
   preparation
Spray Hydrolysis
                                                 Calcining
                                                   Milling
 Hydrated product

  Calcined product
•   "Whiffle balls"
  with nano-sized
      structure
•   Milled slurry
                                                Spray Drying
                                                Micronizing
                         Nano-sized
                           product
                                                                   Source: Reprinted with permission from Verhulst et al. (2003)
      Figure B-3.   Nano-TiCh manufacturing process used by Altair Nanotechnologies, Inc.
      1 Pigment-grade refers to a classification of particles of size 200 run or larger. However, any grade of particles will
       contain a range of particle sizes, and "[a]lthough pigment-grades of TiO2 are usually considered to consist of
       micron sized particles, particles below 100 nm may be present in such grades" (SCCP, 2007).
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 1          Details of the sulfate process, chloride process, and the Altair Process (derived from spray
 2    hydrolysis) are provided in the following paragraphs. The steps unique to each process are presented
 3    first, followed by steps shared in these processes. Additionally, processes specific to manufacturing nano-
 4    TiO2 include an additional gas-phase process (TiCl4 + 2H2O —> TiO2 + 4HC1) and three additional wet
 5    processes (TiOCl2 + 2NaOH -> TiO2 + 2NaCl + H2O ; Na2TiO3 + 2HC1 -> TiO2 + 2NaCl + H2O ; and
 6    Ti(OR)4 + 2H2O -»TiO2 + 4ROH) (Dransfield, 2005). The gas-phase process is similar to the chloride
 7    method except that the titanium tetrachloride is hydrolyzed rather than oxidized. It is also similar in some
 8    aspects to the Altair method. These three wet processes rely on feedstocks that are not found in nature,
 9    and thus require some additional, unspecified preparatory steps. Waste products from the various
10    processes include hydrochloric acid, salt, water, and compounds formed from impurities.
11          Specific Steps in the Sulfate Process.  The sulfate process begins with ilmenite ore (FeTiO3),
12    which is dried, ground, and treated with concentrated sulfuric acid (H2SO4) in an exothermic digestion
13    reaction, producing a cake of titanyl sulfate (TiOSO4) and other metal sulfates.  This cake is then
14    dissolved in water or a weak acid. After chemical flocculation, a clear solution and an insoluble mud are
15    produced.  The clear solution is cooled to crystallize  ferrous sulfate heptahydrate (FeSO4 • 7H2O, known
16    as "copperas").  The ferrous sulfate heptahydrate is separated and sold as a by-product (Millennium
17    Inorganic Chemicals, 2007).
18          The insoluble mud is washed, filtered, and evaporated to produce a concentrated TiOSO4 liquor.
19    The liquor is hydrolyzed to produce a suspension or "pulp" that consists mainly of colloidal hydrous
20    titanium oxide clusters (Millennium Inorganic Chemicals, 2007).
21          The TiO2 is precipitated from the suspension, which is typically facilitated by a seeding technique
22    to control particle  size (no description of the seeding technique was provided). After further washing, heat
23    is applied to crystallize the particles in a process known as calcination, which is also used in other
24    processes. Either anatase or rutile crystals can be produced, depending on the additives applied before
25    calcination (Millennium Inorganic Chemicals, 2007).
26          The following equations represent the chemical processes involved in the sulfate process
27    (Dransfield, 2005):
28                                  FeTiO3 + 2H2SO4 -» TiOSO4 + FeSO4 + 2H2O
29                                         TiOSO4 + H2O -»  TiO2 + H2SO4

30          Specific Steps in the Chloride Process. Natural or synthetic rutile is the feedstock material for
31    the chloride process. During the chlorination step, rutile is added to chlorine and a source of carbon in a
32    fluidized bed  at 900 degrees Celsius (°C).  The exothermic reaction produces titanium tetrachloride
33    (TiCl4) plus a variety of impurities. As the gas cools, low-volatile impurities (e.g., iron, manganese, and
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 1    chromium chlorides) condense out. A stable, very pure liquid TiCl4 is achieved following condensation
 2    and fractional distillation (Millennium Inorganic Chemicals, 2007).
 3          The pure TiCl4 is then oxidized to TiO2 in a second exothermic reaction.  Temperature and other
 4    reaction parameters determine the mean particle size, size distribution, and crystal type of the resulting
 5    TiO2. The TiO2 is cooled, and impurities are removed. Chlorine released by the oxidation reaction is
 6    recycled for reuse (Millennium Inorganic Chemicals, 2007).
 7          The following equations represent the chemical processes involved in the chloride process
 8    (Dransfield, 2005):
 9                                     TiO2 (impure) + 2C12 + C -> TiCl4 + CO2
10                                            TiCl4 +  O2 -> TiO2 + 2C12

11          Specific Steps in the Altair ProceSS-Spray  Hydrolysis. The patented Altair process (Verhulst et
12    al., 2003) was derived from a spray hydrolysis method for TiO2 synthesis. The feed is a titanium
13    oxychloride aqueous solution. The feed solution can be produced by hydrating  liquid TiCl4 in a dilute
14    hydrogen chloride (HC1) solution. In spray hydrolysis, heat (from hot air or a hot receiving surface)
15    causes rapid and complete evaporation of the water in the feed solution as the solution is sprayed. An
16    amorphous, homogeneous, dense, thin film remains  on the receiving surface.  The film is composed of
17    dry, hollow, almost completely amorphous, TiO2 particles containing some free  or hydration water and
18    some HC1 (Verhulst et al., 2003).
19          Calcination for Sulfate and Altair Processes.  Calcination is the process of heating a solid
20    material to a temperature high enough to change its chemical composition (though  generally not high
21    enough to liquefy it). In wet processes like the sulfate and Altair processes, calcination generally occurs
22    after the hydrolysis step. Verhulst et al. (2003) describe the calcined product as a porous crystalline
23    structure of nanoparticles.  The crystalline structure  retains the shape of the original droplets from the
24    hydrolysis step and will eventually be broken down  by milling. The duration and temperature of
25    calcination and the additives introduced during calcination directly influence the structure, particle size,
26    and particle-size distribution of the calcined product. For example, the anatase structure can be  stabilized
27    by adding phosphates during calcination  (Verhulst et al., 2003).
28          Milling and Micronizing for Sulfate, Chloride, and Altair Processes. Milling breaks apart the
29    hollow crystalline lattice 2 structure produced in the calcination step, but has to  be mild enough not to
      2 Lattice is the geometrical arrangement of atoms in a crystal.
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 1    break the individual crystallites (Verhulst et al, 2003). Milling also breaks down agglomerates or
 2    aggregates into smaller particles.
 3          Both a wet media mill (e.g., with zirconia beads) and ultrasonic milling can be effective (Verhulst
 4    et al., 2003). After spray drying, the milled particles ("loosely agglomerated balls") can be "further
 5    micronized to produce a dispersed powder." How, if at all, micronizing differs from milling is not clear.
      B.1.2.  Surface Treatments and Doping
 6          Some, but not all, nano-TiO2 particles used for sunscreen undergo surface treatment to prevent the
 7    creation of free radicals, which could degrade the sunscreen or damage the skin (DuPont, 2007;
 8    Schlossman et al., 2006; Wakefield et al., 2004). Surface coatings for nano-TiO2 in sunscreen can include
 9    combinations of inorganic oxides, simethicone, methicone, lecithin, stearic acid, glycerol, silica,
10    aluminum stearate, dimethicone, metal soap, isopropyl titanium triisostearate (ITT), triethoxy
11    caprylylsilane, and C9-15 fluoroalcohol phosphate.
12          In a patent they hold, Mitchnik and O'Lenick (1996) describe a sample protocol for applying a
13    silicone surface treatment to TiO2 for sunscreen. The patent does not specify the size of the TiO2
14    particles. A quantity of silicone compound (generally between 0.1% and 25% by weight of the total
15    formulation) is combined with TiO2 powder. The mixture is heated to 40-100 °C for 2-10 hours, or long
16    enough to remove 97% of the alcohol produced in the reaction.  The patent holders claim that the
17    resultant coated particles provide superior performance because the coating "preserves the structure of the
18    TiO2 crystals, eliminates the reactivity in water, and makes them hydrophobic."
19          Nano-TiO2 particles  can also be doped with various metals such as manganese,  vanadium,
20    chromium, and iron.  Park et al. (2006) listed examples of doping methods, including: (1) combining
21    particles of a host TiO2 lattice with a second component in solution or suspension, and then baking at no
22    lower than 300 °C. The second component is typically a salt, such as a chloride, or an oxygen-containing
23    anion, such as a perchlorate or a nitrate; (2) mixing solutions of the dopant salt and of a titanium alkoxide,
24    and then heating the solution to convert the  alkoxide to the oxide and precipitate out the doped material;
25    and (3) flame pyrolysis 3 or plasma routes (no additional detail provided).
      3 Flame pyrolysis is a synthesis method in which flame heat is applied to vaporize stock material (gas phase
       precursors) and to initiate chemical reaction for particle (including nanoparticles) production.
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     B.2.  Nano-TiOz Particles and Products Used  in Sunscreens

1         Several commercially-available nano-TiO2 particles intended for sunscreen application and some of
2    their characteristics are summarized in Table B-2 (SCCNFP, 2000). Although these nano-TiO2 particles
3    were selected for their applicability to the European market, they are likely to be fairly representative of
4    nano-TiO2 active ingredients used in the United States.
     Table B-1.    Selected list of nano-TiCh particles used in sunscreen.
Particle name
T805 Degussa20/80
RU/AN
T817Degussa79/12/2
RU/AN/Fe
UV-TitanM160
UV-TitanM212
UV-TitanX161
UV-Titan X200
Eusolex T-2000
TTO 51 A
TT051C
MT-100AQ
MT-100AR
MT-100T-L-1
MT-100SA
MT100TV(orMT-
100TV)
MT100Z(orMT-100Z)
MT-500SA
Mirasun TIW60
UV-Titan M262
Solaveil fine particle
powder
Manufacturer
Degussa
Degussa
Kemira
Kemira
Kemira
Kemira
Merck
Merck
Merck
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Mitsubishi i/Tayca
Rhiodia
Rhiodia and
Kemira
Uniquema
Crystal
type
rutile/
anatase
rutile/
anatase
rutile
rutile
rutile
rutile
unknown
rutile
rutile
rutile
unknown
rutile
rutile
rutile
rutile
rutile
anatase
rutile
rutile
Average crystal
size
21 nm
21 nm
17-20nm
20 nm
15 nm
20 nm
14 nm
35 nm
35 nm
15nm
15nm
15 nm
15nm
15 nm
15nm
35 nm
60 nm
20 nm
10-28nm
Coating materials and concentrations
silicone dioxide <2.5%
silicone dioxide <2.5%(also doped with di-iron trioxide 2%)
alumina 5.5-7.5%, stearic acid 10%
alumina 5-6.5%, glycerol 1%
alumina 8.5-11 .5%, stearic acid 10%
none
alumina 8-11%, simethiicone 1-3%
alumina 11%, silica 1-7%
alumina 11%, silica 1-7%, stearic acid 3-7%
alumina 4-8%, silica 7-11 %
alumina 4-8%, silica 7-1 0%
alumina 3.3-7.3%, stearic acid 5-11%
alumina 4-7.5%, silica 2-4%
alumina 1-15%or3-8%; aluminum stearate 1-13%or 1-15%or
stearic acid 5-1 1 %
alumina 6-10%, stearic acid 10-16%
alumina 1-2.5%, silica 4-7%
alumina 3-7%, silica 12-18%
alumina 5-6.5%, dimethiicone 1-4%
alumina 10.5-1 2.5% or 5-1 5% and silica 3.5-5.5%; alumina 5-15%
and aluminum stearate 5-15%
     nm = nanometer
     Source: SCCNFP (Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers) (2000).
5         Three manufacturers of United States Pharmacopeia (USP)-grade nano-TiO2 for sunscreen
6    applications provided information on their products and processes: Kobo Products Inc., which specializes
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 1    in powders and dispersions; Oxonica, a European nanomaterials group; and Uniqema, a manufacturing
 2    company specializing in oleochemicals 4 and specialty chemicals for cosmetics and personal care
 3    products. Uniqema was acquired by Croda in 2006 (Cosmetics and Toiletries, 2006).
 4          Kobo manufactures a line of 26 attenuation grade TiO2 dispersions containing nano-TiO2. The
 5    primary particle sizes are mostly 10-35 nm in 25 of 26 dispersions; one dispersion contains 90 nm
 6    primary TiO2 particles. The nano-TiO2 aggregate sizes in dispersions (measured by dynamic light
 7    scattering [DLS]) are mostly 103-165 nm in 25 of 26 dispersions, including the dispersion with 90 nm
 8    primary particles; one dispersion contains 230 nm aggregates (Kobo Products Inc., 2009). One of the
 9    Kobo TiO2 dispersions called TNP40VTTS contains nano-TiO2 particles coated with alumina and an
10    isopropyl titanium tri-isostearate/triethyl caprylysilane crosspolymer (Kobo Products Inc., 2009; Shao and
11    Schlossman, 2004).  Polyhydroxystearic acid is used to disperse the product in the solvent/carrier, C12-15
12    alkyl benzoate, which is an ester (Kobo Products Inc., 2009; Shao and Schlossman, 2004).  The particles
13    in another dispersion, CM3K40T4, are surface-treated with alumina and methicone and are dispersed in
14    the cyclopentasiloxane carrier with the help of PEG-10 dimethicone (Kobo Products Inc., 2009; Shao and
15    Schlossman, 2004).
16          Optisol™ UV Absorber, a nano-TiO2 product, is the first commercial product from Oxonica
17    Materials (a branch of Oxonica), and the first commercial health product from Oxonica.  Optisol™ is a
18    powder composed of uncoated rutile nano-TiO2 (size not specified) with approximately 0.67% manganese
19    in the crystal lattice (Kobo Products Inc., 2009; Shao and Schlossman, 2004).  Doping with manganese
20    gives the sunscreen the advantages of increased ultraviolet-A (UV-A) absorption, reduced free radical
21    generation, and increased free radical scavenging behavior (Reisch, 2005; Umicore, 2008).
22          Uniqema/Croda5 manufactures several TiO2 sunscreens, including a line of Solaveil™ Clarus using
23    nano-TiO2 (Chandler, 2006). Solaveil CT-100 and Solaveil CT-200, two of the products in the Solaveil
24    Clarus line, are discussed here  as examples. Solaveil CT-100 has more than 50% C12-C15 alkyl
25    benzoate, 25-50% nano-TiO2, and 1-5% each of aluminum stearate, polyhydroxysteric acid, and alumina
26    (Croda, 2007). Solaveil CT-200 has 15-40% nano-TiO2, 10-30% isohexadecane, 10-30% glycerol tri(2-
27    ethylhexanoate), 3-7% aluminum stearate, and 1-5% each of polyhydroxysteric acid and aluminum oxide
28    (Croda, 2008). The TiO2 particle size distribution is very narrow, with the vast majority of particles
29    falling in the nano range (Croda, 2008).  Uniqema (no date) recommends using CT-200  at a concentration
30    of 2-30%. The dispersion can be included in the oil phase in an oil-in-water (o/w) emulsion, or in the
      4 Oleochemicals, e.g., fatty acids, fatty alcohols, and fatty esters, are derived from biological oils or fats.

      5 Croda acquired Uniqema in 2006 (Cosmetics and Toiletries, 2006).  In this Appendix, information sources are
      cited as it was presented at the time of publication.

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 1   water phase in water-in-oil (w/o) emulsion, or added separately to a w/o emulsion after emulsification
 2   (Uniqema, no date).


     B.3. Formulations for Sunscreen Containing Nano-TiOz

 3         Sunscreen formulations that major manufacturers use are proprietary. Companies that produce
 4   sunscreen ingredients, however, promote their products by publicizing suggested formulations. These
 5   suggested formulations indicate the types of ingredients and processes that might be typical in sunscreen
 6   formulation. Two such suggested formulations are discussed here.
 7         Generally, compatible ingredients are combined into a number of fluid phases.  These phases are
 8   then energetically mixed in a particular sequence (sometimes at specified temperatures) to form an
 9   emulsion. Formulators have to take care not to allow the pH of the mixture to reach the isoelectric point
10   (IEP) of the nano-TiO2 or any other dispersed ingredient.
11         Table B-3 shows a sample formulation using Croda Solaveil CT-100W and Solaveil CT-200
12   (Croda, 2009).  Table B-4 lists a sample formulation that uses nano-TiO2 from Kobo for SPF 35 sunscreen
13   that appears transparent when applied on skin (Kobo Products Inc., 2009).
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          Table B-2.    Formula SC-383-1  for "Weightless Morning Dew with Sun
                          Protection."
Ingredients %
Part A
Water
Hydroxypropyl starch phosphate a
Arlatone V-150 [steareth-100 (and) steareth-2 (and) mannan (and) xanthan gum]
Arlatone LC
Pricerine™ 9088 (glycerin)
Solaveil CT-10W [water (and) titanium dioxide (and) isodeceth-6 (and) oleth-10 (and)
aluminum stearate (and) alumina (and) simethicone]
QS
1.00
0.50
2.00
4.00
5.00
PartB
Solaveil CT-200 [titanium dioxide (and) isohexadecane (and)
triethylhexanoin (and) aluminum stearate (and) alumina (and) polyhydroxystearic acid]
Ethyl methoxycinnamate b
BRIJ™ 721 (steareth-21)
Arlamol PS15E (PPG-15 stearyl ester)
2.00
4.00
2.00
5.00
PartC
Phenoxyethanol (and) methylparaben (and) ethylparaben (and)
propylparaben c
1.00
          pH: 6.75 ± 0.5; viscosity: 223.5 ± 10% (centipoise) cps

          Procedure:

          Disperse Arlatone V-150 in water. Then disperse the preservative. Add Pricerine 9088 and heat to 60 °C and add
          Arlatone LC.  Continue heating to 80°C and add Solaveil CT-10W. Combine and heat Part B to 80 °C.  Add Part B to
          Part A. Homogenize for 2 minutes. Return to stirring and cool to 40 °C. Add Part C. Stir to room temperature.

          Note: QS means a sufficient quantity.
          a Structure XL, National Starch
          "Eusolex 2292, Merck KGaA
          'PhenonipXB.CIariant

          Source: Croda (2009).
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          Table B-3.     Formula KSL-17 for High SPF Transparent Sunscreen.
Ingredients %
Parti
Rose Talc-MS2 - Kobo Products : Talc (and) Methicone
Velvesil 125 - Momentive/Kobo Products : Cyclopentasiloxane (and) C30-45 Alkyl Cetearyl
Dimethicone Crosspolymer
Net-WO - Barnet : Cyclopentasiloxane (and) PEG-10 Dimethicone (and) Disteardimonium
Hectorite
CM3K40T4 - Kobo Products : Cyclopentasiloxane (and) Titanium Dioxide (and) PEG- 10
Dimethicone (and) Alumina (and) Methicone
Uvinul MC80 - BASF : Ethylhexyl ' Methoxycinnamate
Salacos 99 - Nisshin Oil : Isononyl Isonanoate
Lexol EHP - Inolex Chemical : Ethylhexyl Palmitate
Squalane - Fitoderm : Squalane
Tocopherol - Cognis : Tocopherol
SF96-350 - Momentive/Kobo Products : Dimethicone
SF96-100 - Momentive/Kobo Products : Dimethicone
SF1202 - Momentive/Kobo Products : Cyclopentasiloxane
Propyl Paraben NF - International Sourcing : Propylparaben
1.00
3.00
0.20
35.00
7.00
5.00
4.00
0.20
0.20
1.00
1.00
27.10
0.10
Part 2
Sodium Citrate - Roche : Sodium Citrate (and) Water
Net-DG - Barnet : Dipotassium Glycyrrhizinate
Sodium Hyaluronate - Centerchem : Sodium Hyaluronate (and) Water
Keltrol CG-T - CP Kelco : Xanthan Gum (and) Water
Butylene Glycol - Ruger : Butylene Glycol
Methyl Paraben NF - International Sourcing : Methylparaben
Water
2.00
0.10
1.00
2.00
4.00
0.10
6.00
          Manufacturing Procedure:
          * Use explosion-proof mixers and equipment during batching process *
               1. Mix each Part separately.  Make sure Net-WO is dispersed in Part 1.
               2. Heat both Parts to 40 °C and add Part 2 to Part 1 while stirring with homogenizer at 3,000 rotations per
                   minute (rpm).
               3. Increase the rotation to 5,000 rpm and continue to emulsify for 5 minutes.
               4. Cool down to room temperature with sweeping mixer.
          Source: Kobo Products Inc. (2009).
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B.4. References

Chandler M. (2006). Innovative UV Protection - Creating your advantage in sunscreen products.
       Unpublished PowerPoint presentation. Uniqema/Croda.
Chen X; Mao SS. (2007). Titanium dioxide nanomaterials: Synthesis, properties, modifications, and
       applications. Chem Rev 107: 2891-2959.

Cosmetics and Toiletries. (2006, July 3, 2006). Croda Invests in Growth: Acquires Uniqema. Retrieved
       July 17, 2009, from
       http://www.cosmeticsandtoiletries.com/networking/news/company/3269231.html.
Croda. (2007). Composition Declaration of Solaveil CT-100.
Croda. (2008). Chemical Composition of Solaveil CT-200.
Croda. (2009). Weightless Morning Dew with Sun Protection (SC-383-1). April 21, 2009.

Dransfield G. (2005). Manufacture of novel, transparent TiO2 based sunscreens. Retrieved May 9, 2008,
       from http://www.wun.ac.uk/nanomanufacturing/archive/05_06_series/documents/dransfield.pdf.
DuPont. (2007). Nanomaterial Risk Assessment Worksheet DuPont™ Light Stabilizer. Last Updated June
       21, 2007. Retrieved June 18, 2008, from
       http://www.edf.org/documents/6913_TiO2_Worksheet.pdf
Hext PM; Tomenson JA; Thompson P. (2005). Titanium dioxide: Inhalation toxicology and
       epidemiology. Ann Occup Hyg 49: 461-472.
Kobo Products Inc. (2009). Attenuation Grade TiO2 Dispersions - ref TiD-001 / [February 5, 2009 High
       Solids and High Speed Dispersions Technical Literature] South Plainfield, NJ; Kobo Products,
       Inc. Retrieved July 16, 2009, from http://www.koboproductsinc.com/Downloads/Kobo-
       TiO2Dispersions.pdf
Millennium Inorganic Chemicals. (2007). TiO2 Processes Compared. Retrieved February 16, 2009, from
       http://www.millenniumchem.com/NR/rdonlyres/B753C492-F08B-4DBl-BBC6-
       88058279FBFF/0/Figurel01TiO2F.pdf
Mitchnick M; O'Lenick AJ, Jr. (1996). U.S. Patent #5565591, class: 556/10. Silicone polymers for the
       modification of titanium dioxide.
Osterwalder N; Capello C; Hungerbiihler K; Stark WJ. (2006). Energy consumption during nanoparticle
       production: How economic is dry synthesis? J Nanopart Res 8: 1-9.
Park GB; Knowland JS; Flutter BR (2006). U.S. Patent #20060134026, class: 424/59. Sunscreens.
Reisch M. (2005). New-wave sunscreens. Chemical and Engineering News 83: 18-22.

SCCNFP (Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers).
       (2000). Opinion of the scientific committee on cosmetic products and non-food products intended
       for consumers concerning titanium dioxide. Brussels, Belgium.
SCCP (Scientific Committee on Consumer Products). (2007). Preliminary opinion on safety of
       nanomaterials in cosmetic products. Retrieved April 4, 2008, from
       http://ec.europa.eu/health/ph_risk/committees/04_sccp/docs/sccp_o_099.pdf
Schlossman D; Shao Y; Detrieu P. (2006). Perspectives on supplying attenuation grades of titanium
       dioxide and zinc oxide for sunscreen applications. Public meeting on nanotechnology materials in
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       FDA regulated products. Last updated October 10, 2006. Retrieved October 28, 2007, from
       http://www.fda.gov/nanotechnology/meetings/kobo_files/textmostly/index.html.

Shao Y; Schlossman D. (2004). Discovering an optimum small micropigment for high UV shielding and
       low skin whitening. Retrieved April 4, 2008, from
       http://www.koboproductsinc.com/Downloads/IFSCC2004.pdf

Umicore. (2008). Nano-sized oxide powders for UV applications. Paper presented at the Innovation for
       Sustainable Production (i-SUP2008), April 22-25, Bruges, Belgium.

Uniqema. (no date). Solaveil CT-200. Uniqema, London. PC/E/03-03/GLOB/14.5/CT200.

Verhulst D; Sabacky BJ; Spitler TM; Prochazka J. (2003). Process for the production of nano-sized TiO2
       and other ceramic oxides by spray hydrolysis.

Wakefield G; Lipscomb S; Holland E; Knowland J. (2004). The effects of manganese doping on UVA
       absorption and free radical generation of micronised titanium dioxide and its consequences for
       the photostability of UVA absorbing organic sunscreen components. Photochem Photobiol Sci 3:
       648-652.
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       Appendix  C.  Nano-TiOz  Exposure Control

               in the Workplace and Laboratory


     C.1.  Workplace Exposure Controls

 1        This section summarizes strategies that are currently in place or recommended to decrease
 2   exposures to nanomaterials in the workplace (Nanosafe, 2008b; NIOSH, 2009) and to ensure the
 3   effectiveness of personal protective equipment (PPE) against nano-TiO2 (Golanski et al., 2008; Guizard
 4   and Tenegal, 2008; Nanosafe, 2008b). While this section focuses on workplace practice of nanomaterial
 5   manufacturers, some of the principles and use of PPE are also applicable to laboratories and other
 6   settings.
 7        The NanoSafe dissemination report (Nanosafe, 2008b) provided several tiers of approaches to
 8   decrease nanomaterial exposure in the workplace. During production, the first and preferred approach is
 9   to avoid free air flowing particles. If this avoidance is not possible, the process should be contained. If
10   process containment is not possible, extended PPE (which includes double gloves of nitrile, a mask [FFP3
11   or powered respirators incorporating helmets], a protective suit, and safety shoes) and an effective local
12   exhaust system, such as a high efficiency particulate air (HEPA) H14 filter, should be used.
13        During  loading and unloading of reactors, and while packing containers, exposure can be decreased
14   by process containment (e.g., by using a glove box or emptying the reactor using an industrial vacuum
15   with a HEPA filter through a liquid trap) (Nanosafe, 2008b).  Less preferred alternatives are to transfer
16   nanoparticles within a laminar air-flow booth or extraction hood, or to conduct the transfer in an isolated
17   area equipped with HEPA H14 filter. These alternative options would require the use of extended PPE
18   (Nanosafe, 2008b).
19        During  cleaning, special vacuums to avoid dust explosion can be used to trap nanoparticles. The
20   vacuums should be cleaned in a room equipped with a HEPA H14 filter and a washer to clean the
21   protective suites (Nanosafe, 2008b). Alternatively, particles  can be drawn into a powder-collection
22   system using a variable-speed fan. Components should be cleaned in a hood equipped with a HEPA filter
23   and an explosion vent panel.
24        The National Institute for Occupational Safety and Health (NIOSH) has a nanotechnology program
25   to increase safety and decrease potential exposures to nanomaterials in the workplace (NIOSH, 2009). In
26   a NIOSH document for safe nanotechnology (NIOSH, 2009), occupational health surveillance and
27   guidelines for working with engineered nanomaterials are  discussed, among other topics.  Some of these
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 1    programs could also encourage the general public to reduce environmental releases. Some companies
 2    that manufacture nanoscale titanium dioxide (nano-TiO2) have engineering safeguards and additional
 3    programs in place to reduce or eliminate occupational and environmental exposures (e.g., BASF, 2008;
 4    DuPont, 2007). Various production methods to decrease worker exposure are also being investigated [for
 5    nano-TiO2, see Guizard and Tenegal (2008)].
 6          With a goal to manage nanotechnology safely and effectively within industry, the Nanoparticle
 7    Occupational Safety and Health (NOSH) Consortium has investigated methods for monitoring workplace
 8    exposure and testing protective technologies.  The NOSH Consortium has measured the effectiveness of
 9    standard respiratory filters with silicon dioxide (SiO2) aerosol nanoparticles. With the exception of
10    prolonged exposure (400 minutes or longer), the filter efficiencies for both charged and re-neutralized
11    SiO2 aerosol nanoparticles met the specifications of the filter type (Ostraat, 2009).  The longest exposure
12    time within which the N100 filter performed at or exceeded the efficiency specified by the filter ranking
13    (>99.97-percent filtration efficiency) was 210 minutes (Ostraat, 2009). No PPE specific for
14    nanomaterials exists or is under development (Klaessig, 2008). (For filter efficiency against nano-TiO2
15    aerosol penetration tested by NanoSafe, see below.)
16          In the following section, two types of PPE are briefly discussed in terms of their protection against
17    nano-TiO2 aerosols: 1) filters for inhalation protection and 2) protective clothing and gloves for skin
18    protection.  Eye-protective gear is available as a third type of PPE commonly used  for protection against
19    nano-TiO2 aerosols, but no information was found on this subject.
20          Each type of nanomaterial is different, and the methods for testing PPE efficiency (such as using
21    charged or neutralized particles) could greatly affect the measured barrier effectiveness.  For example,
22    fibrous filters often remove more  charged aerosol nanoparticles than uncharged or neutralized aerosol
23    nanoparticles (Kim et al., 2006; Ostraat, 2009). Other physicochemical properties of nanoparticles that
24    affect filtration efficiency include size, chemical composition, and shape. The size of the particle that
25    penetrates most effectively into a  specific filter is called the maximum penetrating particle size  (MPPS).
26    For particles smaller than the MPPS, the particle penetrations decrease with decreasing particle size; for
27    particles larger than the MPPS, the particle penetrations decrease with increasing particle size.  Particles
28    smaller than the pore size of the filter may be filtered out when the Brownian movement of the  particles
29    leads to collision of the particle and filter [page 400 and 401 of McKeytta (1984)].
30          Electrostatic filters are charged polypropylene fibers, classified as FPP3—minimum filtration
31    efficiency 99%—based on European Norm (EN) certification. When an electrostatic filter was  tested
32    with nano-TiO2 aerosols, for which size ranged from 16 nm to greater than 76 nm, the  MPPS was
33    approximately 35 nm, which was  very similar to graphite MPPS (Golanski et al., 2008).  At the MPPS,
34    however, nano-TiO2 penetration was nearly five times higher than that for graphite. Near the MPPS, the
35    differences between nano-TiO2 and graphite particle penetration increase by an order of magnitude.
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 1         HEPA filters have a minimum filtration efficiency of 99.97%, are composed of glass fibers, and are
 2    classified as H12 for particles <1 micrometer (|im). Like electrostatic filters, HEPA filters showed one
 3    order of magnitude higher penetration of nano-TiO2 (10-19 nm) than that of graphite (10-19 nm), with
 4    the highest penetration at approximately 0.2% for 19-nm TiO2 (Golanski et al., 2008). The penetration of
 5    platinum (Pt) through HEPA filters was only slightly lower than that of nano-TiO2.  Golanski et al.
 6    showed that particle size alone might not be a sufficient indicator of HEPA filter performance and
 7    suggested that nano-TiO2 might penetrate fibrous filters more than other nanomaterials, namely graphite
 8    and Pt (2008). The exposure duration of the Golanski et al. (2008) study was not reported, and therefore,
 9    it could be possible that the filtration efficiency of HEPA filters for nano-TiO2 might decrease with
10    prolonged exposure, as was found for the N100 filter for more than 400 minutes of exposure to SiO2
11    aerosol nanoparticles (Ostraat, 2009).
12         The efficiency of protective clothing in preventing nano-TiO2 penetration by diffusion was higher
13    for non-woven fabric than woven cotton and polyester fabric (Golanski et al., 2008). Air-tight, non-
14    woven, polyethylene Tyvek (115 (im thick) was more efficient against nanoparticle penetration than
15    woven cotton (650 (im thick) and woven polyester (160 (im thick) for 10-nm nano-TiO2 (Golanski et al.,
16    2008), 10-nm nano-Pt (Golanski et al., 2008), and 40- and 80-nm graphite (Nanosafe, 2008a).
17         Nitrile, latex, and Neoprene gloves were reported to be efficient against nano-TiO2 aerosol
18    penetration via diffusion for a short exposure time (minutes). No penetration through gloves was detected
19    when the gloves were exposed to aerosols of approximately 10-nm nano-TiO2 and 10-nm Pt (Golanski et
20    al., 2008) or 20- to 100-nm graphite (Nanosafe, 2008a). As these authors pointed out, aerosol penetration
21    test results that examine diffusion do not indicate penetration against dispersion. In addition, continuous
22    flex of gloves could lead to  cracks and holes in the gloves (Schwerin et al., 2002), so changing gloves
23    throughout the day is recommended (Harford et al., 2007).


      C.2.  Manufacturer  and  Laboratory  Practices

24         In 2006, the University of California-Santa Barbara completed a study of nanomaterial
25    manufacturers and laboratories for the International Council on Nanotechnology by surveying
26    organizations about their manufacturing and laboratory practices. Survey results indicated that only 36%
27    of the 64 responding organizations stated that they monitored exposure to the nanomaterials in their
28    workplace. Additionally, 38% of the organizations surveyed believed their nanomaterials posed no
29    special risks, 40% had safety concerns, and 22% were unaware whether the materials they work with or
30    manufacture pose safety risks (Gerritzen et al., 2006).
31         Subsequently, the same research team published additional findings based on a larger sample size.
32    Of the 82 responding firms and laboratories, 89% had a general environmental health and safety program,

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 1    and 70% provided some type of special training on nanomaterial safety. Nanomaterial safety training was
 2    more prevalent in North American firms and laboratories (88%) than in European (64%) or Asian (61%)
 3    organizations. Nearly 82% of respondents made nano-specific PPE recommendations to employees.
 4    Those tended to be the same firms and laboratories that used advanced engineering controls (i.e., beyond
 5    fume hoods) to prevent exposure. Controls included exhaust filtration, air filtration, wet scrubbers, and
 6    automated or enclosed operations. Approximately 56% of North American respondents practiced
 7    workplace monitoring for nanoparticles, compared to 32% of all respondents. Waste-containing
 8    nanomaterials were disposed of as hazardous waste in 78% of North American organizations, compared
 9    to 60% of all respondents (Conti et al, 2008).
10         A survey of 43 New England nanotechnology firms found that larger companies (with 500 or more
11    employees) tended to better recognize environmental health and safety (EHS) risks potentially posed by
12    nanoparticles and had EHS measures in place. Many smaller firms either did not perceive risks or did not
13    implement EHS measures (due  both to staff and resource constraints and a lack of information on how to
14    quantify nanoparticle risks) (Lindberg and Quinn, 2007).
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C.3. References

BASF. (2008). Guide to safe manufacture and for activities involving nanoparticles at workplaces in
       BASF AG.  Retrieved June 2, 2006, from
       http://www.basf.eom/group/corporate/en/function/conversions:/publish/content/sustainability/dial
       ogue/in-dialogue -with-
       politics/nanotechnology/images/BASF_Guide_to_safe_manufacture_and_for_activities_involvin
       g_nanoparticles.pdf
Conti JA; Killpack K; Gerritzen G; Huang L; Mircheva M; Delmas M; Harthorn BH; Appelbaum RP;
       Holden PA. (2008). Health and safety practices in the nanomaterials workplace: Results from an
       international survey. Environmental Science & Technology 42: 3155-3162.

DuPont. (2007, June 21, 2007). Nanomaterial Risk Assessment Worksheet DuPont™ Light Stabilizer.
       Retrieved June 18,  2008, from http://www.edforg/documents/6913_TiO2_Worksheet.pdf
Gerritzen MA; Lambooij E; Stegeman JA; Spruijt BM. (2006). Slaughter of poultry during the epidemic
       of avian influenza in the Netherlands in 2003. Veterinary Record 159: 39-42.

Golanski L; A. Guiot; Tardif F. (2008). Experimental evaluation of individual protection devices against
       different types of nanoaerosols: graphite, TiO2 and Pt. Retrieved March 22, 2009, from
       http://www.nanosafe2008.org/home/liblocal/docs/Oral%20presentations/O6-l_Golanski.pdf

Guizard B; Tenegal F. (2008). Liquid Recovery of TiO2 nanoparticles snythesized by laser pyrolysis.
       Retrieved March 30, 2009, from
       http://www.nanosafe2008.org/home/liblocal/docs/Oral%20presentations/O6-4_Guizard.pdf

Harford AJ; Edwards JW; Priestly BG; Wright PFA. (2007). Current OHS Best Practices for the
       Australian Nanotechnology Industry, from http://mams.rmit.edu.au/72nuxiavskpg.pdf

Kim CS; Bao L; Okuyama K; Shimada M; Niinuma H. (2006). Filtration efficiency of a fibrous filter for
       nanoparticles. Journal of Nanoparticle Research 8: 215-221.

Klaessig F. (2008). Personal Communication. "Comments on EPA Draft Case Study #1: Nanoscale
       Titanium Dioxide for Water Treatment. Attachment to email to J. M. Davis, U.S. EPA." January
       2.  Parsippany, NJ: Degussa Corporation.

Lindberg JE; Quinn MM. (2007). A Survey of Environmental, Health and Safety Risk Management
       Information Needs  and Practices among Nanotechnology Firms in the Massachusetts Region
       (Project on Emerging Nanotechnologies). Woodrow Wilson International Center for Scholars,
       Washington, DC.

McKeytta JJ.  (1984). Encyclopedia of Chemical Processing and Design (Vol. 21): CRC.

Nanosafe.  (2008a). Dissemination Report: Are  conventional protective devices such as fibrous filter
       media, respirator cartridges, protective clothing and gloves also efficient for nanoaerosols?
       Retrieved June 2, 2009, from
       http://www.nanosafe.org/home/liblocal/docs/Dissemination%20report/DRl_s.pdf

Nanosafe.  (2008b). Dissemination Report: First results for safe procedures for handling nanoparticles.
       Retrieved June 2, 2009, from
       http: //www .nanosafe. org/scripts/home/publigen/content/templates/show. asp ?P=63 &L=EN&ITE
       MID=13.
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NIOSH (National Institute for Occupational Safety and Health). (2009). Approaches to Safe
       Nanotechnology:  Managing the Health and Safety Concerns Associated with Engineered
       Nanomaterials. Retrieved June 2, 2009, from http://cdc.gov/niosh/docs/2009-125/.
Ostraat ML. (2009). Industry-led initiative for occupational health and safety. In Hull M, Friedrichs S
       (Eds.), Risk Governance of Nanotechnology: Environmental, Health and Safety Concerns:
       William Andrew Pub.
Schwerin MR; Walsh DL; Coleman Richardson D; Kisielewski RW; Kotz RM; Routson LB; David Lytle
       C. (2002). Biaxial flex-fatigue and viral penetration of natural rubber latex gloves before and after
       artificial aging. Biomedical Materials Research 63: 739-745.
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