&EPA
                        United States
                        Environmental
                        Protection
                        Agency
                  Office of
                  Solid Waste and
                  Emergency Response
Publication 9345.0-051
May 1992
ECO   Update
Office of Emergency and Remedial Response
Hazardous Site Evaluation Division (OS-230)
                                                    Intermittent Bulletin
                                                   Volume 1, Number 4
Developing A Work Scope  For Ecological
Assessments
  This  Bulletin  is  intended  for  Remedial  Project
Managers (RPMs), to help them plan and manage
ecological assessments of sites as part of the Remedial
Investigation and Feasibility Study (RI/FS) process. : As
used here, the generic term work scope describes the
process of specifying the work to be done for ecological
assessment, as part of the overall RI Work Plan. The term
encompasses project scoping, development and approval
of the Work Plan, and preparation of the Statement of
Work (SOW) for contractors (at Fund-lead sites).

  The outcome of a successfully executed work scope
should be an ecological assessment that includes four
essential components:   problem formulation, exposure
assessment,  ecological effects  assessment,  and  risk
characterization.  A work scope should also provide for
close oversight of individual tasks.  This will ensure that
the  assessment  accomplishes  its objectives  within
reasonable budget and schedule limitations.
Need  for Clarity,  Specificity,  and
Completeness

   SOWs and Work Plans should clearly state the studies
needed at each phase of the assessment. In addition, they
should include other parameters concerning an assessment,
such  as sample collection, data analysis,  and reports.
Specifically, SOWs and Work Plans should describe:
   1 Although the primary focus of this document is on the RI/FS
process, On-scene Coordinators may find much of the information useful
in evaluating sites during the removal process.

    Ecological Assessment ofSuperfund Sites: An Overview (ECO
Update Vol., 1, No. 2).
                           •   Which studies should be conducted;
                           •   Why they should be conducted;
                           •   When and where they should be conducted;
                           •   What data should be collected;

                           •   How samples should  be  collected, handled,  and
                              analyzed;
                           •   How data should be evaluated; and

                           •   What reports should be produced.


                                     IN THIS BULLETIN

                           The Role Of The Biological Technical Assistance
                           Group	2
                           Points To Consider In Developing A Work Scope	2
                           Elements  Of  An  Ecological  Assessment  Work
                           Scope	4
                           Ensuring Contractor Capability To Do Work	7
                           Review Of Interim And Final Products	8
                           Sample Work Scope	8
                           Conclusion	9
                           Appendix	10
                           Preparing  a  clear,  specific,  and
                           thorough   SOW  will  avoid  such
                           problems  as the following:
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    •   Too much work,
    •   Too little work,
    •   Incorrect work,  and
    •   Inadequate  QA/QC.
    The work scope should also detail how decisions will
be made about the need for additional studies.

   Preparing a  clear,  specific, and  thorough  SOW will
avoid such problems as the following:

   •   Too much  work.    In  the absence  of clear
       direction, a contractor may do considerably more
       work than is required to characterize the ecological
       risks at the  site, wasting  both time and money.
       The studies could be  valid, well-designed, and
       complete, but unnecessary given the nature of the
       site and its contaminants.

   •   Too little work.  An  improperly designed study
       can result in inadequate  attention to potentially
       important habitats or  species associated with the
       site, too few sampling stations to characterize the
       habitat,  or  too few  data points for  meaningful
       statistical  analysis.    Such  shortcomings  could
       result in the need to conduct additional studies and
       cause delays in producing an acceptable RI/FS.

   •   Incorrect  work.   If  the  SOW  is  not  specific
       enough  as to what work  is  needed or what the
       objectives of the  studies are, the contractor may
       conduct studies that fail to meet the needs of the
       RI/FS  decision-making process.   In  this case,
       valuable time may be lost as the correct studies are
       rescheduled.

   •   Inadequate  QA/QC.  If the SOW does not specify
       data quality objectives (DQOs) then, the data may
       not meet the  level of quality  required to make
       decisions on risk or remedial actions.  As above, a
       delay in the RI/FS process may result.


The Role  of  the  Biological Tech-
nical Assistance  Group (BTAG)

   Most EPA Regional Offices have established groups of
biologists to advise site managers on ecological assessment
in the RI/FS from  the Work  Plan stage  onward.  These
Biological Technical Assistance Groups (BTAGs)3 provide
valuable help in the development of a a work scope.
   3 These groups are sometimes known by different names, depending
on the Region, and not all Regions have established BTAGs. Readers
should check with the appropriate Superfund manager for the name of the
   RPMs should contact the Regional BTAG Coordinator
as early in the process as possible,  certainly before the
Work Plan has been developed. The  RPM should provide
appropriate documentation on the site and its contaminants
to BTAG members before the group  meets to discuss the
site.    In  addition,  the  BTAG may find a  brief oral
presentation on the site and its history helpful at this time.
(A future ECO  Update will provide  guidance on how to
provide the BTAG with  useful information in this initial
briefing.)  Following  this initial review of site data,  the
BTAG can make recommendations on the need for studies
to characterize  the ecological risks posed by the site.
When  the draft Work Plan has been developed,  BTAG
review may elicit further helpful comments.

   The BTAG  should  also  be consulted when interim
products  (reports,  data  summaries,  etc.) are delivered.
Based  on the data in such  a product,  the BTAG may
recommend modifications  to the original work  scope.
Because this kind of "mid-course correction" can save a
project time  and  money, the RPM  is  well  advised  to
schedule time for such reviews in the Work Plan.


Points  to Consider in Developing a

Work Scope


Definition  of Objectives

    The work scope for the ecological  assessment of a
Superfund site requires an overall objective to provide the
assessment with direction.  When an assessment has a
clear  objective,  the RPM can readily  determine which
studies will further the assessment. For example, at a site
where chemicals from mine tailings contaminated the cold
mountain streams that flow through the area, the work
scope had for one of its  objectives to determine whether
resident fish had suffered adverse impact. Consequently,
the work scope  specified studies that concerned fish and
their environment.  These studies included aquatic toxicity
tests,  a fish survey, and bioaccumulation4 studies using
resident fish.

   The overall assessment objective may be clear from the
outset, based on  data from  previous studies or  on an
evaluation of  the concentrations and known effects of site
contaminants.    More likely,  some  preliminary  studies,
including a site visit and collection of screening-level data,
will be needed to identify and specify the objective of the
ecological assessment. Where possible, these preliminary
studies should incorporate the need for future work.
BTAG Coordinator or other sources of technical specialized facilities,
and specialized equipment necessary to carry out the work.  If not,
qualified subcontractors should be sought for those tasks where their
qualifications are needed.

   4  Bioaccumulation  is the accumulation of a substance in an
organism's tissues as a result of respiration, absorption, or feeding.
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   Just as an ecological assessment gains direction from
having an overall objective, each study that the work scope
specifies also should have a clear objective, such as filling
a data gap or testing a hypothesis about the effects of the
site's contaminants on resident organisms. By stating a
study's objective, an RPM provides guidance for designing
the study. For example, the work scope for the mining site
described above called for  aquatic  toxicity  testing to
determine whether the water  was toxic to freshwater fish
that  thrive at low temperatures.   This  study  objective
provided specific direction in planning the toxicity tests.

Assessment Design

   The work scope lays out  the design for an ecological
assessment.  Assessment designs vary tremendously from
site to site depending on:

   •    The objective and the assessment;

   •    The size, location, and accessibility of the site;

   •    The  site's ecology—what is already  known and
       what needs to be known; and

   •    The site' s contaminant history.

   In an ecological assessment, the individual studies are
the pivotal elements.  If the overall  objective  gives  an
ecological  assessment a purpose, the  studies  are  the
vehicles by  which it  attains its purpose.   Studies can
include  chemical  analysis of  media  or biota,  toxicity
testing of laboratory or resident organisms, biological field
studies,  and  analyses of organisms' physiological  or
pathological condition. However, because a work scope
indicates only those studies necessary for assessing  a
specific site,  any one  assessment need not include all of
these types of studies.  The assessment design specifies not
only which studies to perform but also the level of effort
for each. For example, the work scope developed for the
mining site described above included toxicity testing, but
only of one medium (surface water)  and with only one
type of test organism (fathead minnow).  At another site,
toxicity testing might include evaluation of soil, sediment,
and surface water using several different organisms.

    The complexity of an ecological assessment makes it
essential that trained ecologists have responsibility for its
design.  The  RPM can consult the BTAG for advice as to
which media  to analysis, which studies to perform, and at
what  level  of effort.   The   RPM  can include  this
information in the SOW.  As discussed below, since the
contractor has responsibility for developing the Work Plan
from the SOW, the RPM needs to  consider whether the
contractor's  staff has the required expertise.   After a
contractor has prepared the  Work  Plan  the BTAG can
review it and advise the RPM whether or not to approve it.
The phased approach ensures that:
       •   Only  the  necessary  work
           will be done, and
       •   All the  necessary work will
           be done.
Phased        Approach
Implementation
to
Task
   For most sites, a phased approach with expert review at
each phase results in the most efficient use of resources.
With the phased approach data or observations from one
phase determine whether further  studies  are needed  to
meet the assessment's objectives  and, if so,  what these
studies are.  At some sites, the phased approach might
result in  a low level of effort adequately characterizing
ecological risks.  At others, the phased approach might
indicate that the assessment should be expanded to include
studies of specific habitats of contaminants  in order  to
evaluate the risks. At still other sites, the phased approach
could identify  areas originally not considered at risk.  In
this case, the RPM would want to expand the  work scope
to include  an  assessment  of  the  newly  identified  area.
Review of interim products, such as a report on the levels
of contaminants of concern or a field survey of resident
species, can contribute to the  phased approach.  Careful
review of interim products can help to ensure that the
assessment  remains focused  on those studies  most
important for evaluating the site's ecological effects.

   To summarize, the phased approach ensures that:

   •   Only the necessary work will be done, and

   •   All the necessary work will be done.

   The value of the phased approach can sometimes be
outweighed by  other factors.  For example,  seasonality
affects when certain types of studies, such  as  loristics
surveys, can occur.  In some cases, budgetary restrictions
and time constraints may be incompatible with the phased
approach.  RPMs may need to  consider such factors when
planning studies.

   In practical terms, the phased approach requires an
RPM to decide when a contractor should proceed from one
task to the next and whether the contractor should proceed
with one alternative task or  another.  In making these
determinations,  the  RPM interprets  information  from
completed  studies.  The BTAG can assist the RPM  in
identifying  criteria appropriate for evaluating  data.   (See
Figure 1.)
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An  ecological  assessment  should  be
designed   to   contribute   to   remedial
decisions at the site.
   As an example of the phased approach, consider the
following hypothetical case. (See Figure 1.)  An RPM has
a field reconnaissance done in order to identify and map
potentially exposed habitats at a site.  The RPM then uses
the results  of this  study  to decide on the number and
placement of sampling stations for initial chemistry data.
After the first round of sampling  for contaminant levels at
these  stations,  the  chemistry  data   indicates  that
contaminant levels are high enough in some areas of the
site to warrant collection of biological data from the field,
along with additional data on site chemistry.   The field
data collected indicate the advisability of toxicity testing at
certain stations, but not at others.  In other parts of the site,
the low level of contaminants indicate that now further
biological   investigation   is  required.    Thus,   in  this
hypothetical example, use of the phased approach results
in there as most in need of study receiving the most
attention.
   An  ecological  assessment  involves
problem formulation,  exposure assess-
ment, ecological effects assessment, and
risk characterization.
Relating    Ecological    Information     to
Remedial Decision-Making

   While  an ecological assessment of a  Superfund  site
might extend our knowledge  of the environment and the
effects of  contaminants  on  it, the assessment is  not
intended  as a research  project.   Rather, it should be
designed to contribute to  remedial decisions at the site.
Ecological  assessments serve this function  when they
determine  whether remediation  is needed, indicate the
conditions (if any  exist)  requiring remediation, suggest
technologies for achieving remediation, and/or estimate the
environmental effects  of  proposal remedial  alternatives.
At the earliest stages of Work Plan development, the RPM
and  BTAG  should consider what types of ecological
information will contribute to remedial decisions.   For
example, the site manager may need to know:
   •   If   remediation    goals   are    protective   of
       environmental receptors,5

   •   If  ecological  risk considerations  will affect the
       definition of the area to be remediated,

   •   If  special  measures  need to be taken during
       remediation to protect natural habitats, and

   •   What  monitoring  will  be  needed  to  ensure
       protection of environmental receptors during and
       after remediation and to evaluate the effectiveness
       of remedial actions.

   Questions such  as these should form part of the initial
scoping session,  where  the RPM and the BTAG  select
appropriate studies  and study designs.
Elements     of     an     Ecological
Assessment Work Scope

   As described in Ecological Assessment of Superfund
Sites: An  Overview  (ECO Update  Vol.  1, No.  2)  an
ecological   assessment  involves  problem  formulation,
exposure assessment, ecological  effects  assessment, and
risk characterization. To ensure that an assessment fulfills
its objectives, the work scope should use its elements to
accomplish these tasks. In addition, the work scope should
identify  data quality indicators to ensure that established
DQOs are met.

Problem Formulation

   Problem   formulation  defines  the   assessment's
objectives  and also involves a thorough description of the
site.   This  qualitative  description  must occur before
deciding on any substantial quantitative work.

   An initial site description should include citations from
existing   site   literature   (such   as   the   Preliminary
Assessment,  Site Inspection, or any studies conducted in
support  of  removal  actions) relating  to  site history,
physical features of the site, species expected at or near the
site, and known or anticipated effects of site contaminants
on receptors.   Investigators  should  determine whether
threatened or endangered species are known or suspected
to occur at or near the site.  Descriptions of potentially
affected habitats should include as much detail as possible.
For instance, stream habitats vary considerably depending
on stream  depth and  width, type of  stream bottom, aid
types of  vegetation  in  and adjacent  to  the stream.
Information pertaining to these types of characteristics
could affect both the kinds of studies required to evaluate
possible effects and the level  of effort needed to conduct
the studies.
   5 Receptors are individuals, populations, or communities/habitats that
may be exposed to a contaminant.
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    This qualitative description of the site helps to indicate
whether further studies are needed and, if they are, what
these  studies  should  be.   For  example,  if  scientific
literature or databases indicate  that a site's  contaminant
concentrations consistently fall below levels likely to cause
adverse  ecological  effects,  additional analyses  may be
unnecessary.     On   the  other  hand,  if  contaminant
concentrations suggest a need for further investigation, the
initial site  description may identify potential  exposure
routes useful in targeting the additional studies to media
and areas of greatest concern.  Targeting studies make the
most efficient use of the time and  money available for the
ecological assessment.

    A  site  visit  should  form  part  of  the  initial  site
description phase.  In addition, the RPM may decide to
characterize the  site's ecology  further  by  conducting
limited field studies.   These studies  could include aerial
photography,  evaluation  of  habitats'   suitability  for
wildlife, functional evaluation of wetlands,6 qualitative or
semi-quantitative  examination  of the  environment for
evidence of stress (e.g., stressed or dead vegetation, bare
soil and erosion, dominance by pollution-tolerant species),
and field verification of the presence of absence of key
species.  At some sites, the existence of site descriptions
made  prior  to  contamination  may  enable  the RPM to
assemble a "before and after" picture of the site.
                                  Figure T. The Phased Approach
                                  As this hypothetical cccici&csi
                                  assessment llustrstes, thephsGvct
                                  appn&ch results r> ths arsss most
                                  ri need of stud/ recer/rig the most
                                  attention.
    6 A functional evaluation of wetland determines the importance of
the wetland for such values as wildlife habitat, pollution abatement, and
flood control.  This type of study helps to establish the value of a
particular wetland as it relates to the need for remediation. Another type
of study, a wetland delineation, defines the boundaries of a wetlandbasad
on soil type, vegetation, and hydrology. The delineation aids in the
selection and evaluation of remedial alternatives. Site managers should
consult with their BTAGs to determine which of these studies are
appropriate, if at all, and when they should be conducted.
         Based on the information developed in this initial site
      description, the investigator (under the direction  of the
      RPM and with BTAG consultation) should specify:

         •   The receptors (habitats and species) most likely to
             be exposed to site contaminants,

         •   The contaminants most likely to be of ecological
             concern,

         •   The ecological effects most likely to be important
             with regard to the site, and

         •   The  studies  needed  to  characterize  actual  or
             potential   adverse  effects  associated  with  site
             contaminants   and,   where    applicable,   the
             hypothesis that the study will test.

      Exposure Assessment

         Since  exposure  assessment  quantifies the actual  or
      potential exposure of receptors to contaminants, the work
      scope must plan for studies that gather appropriate data on
      both receptors and contaminants.  Evaluation of chemical
      and biological data  will  indicate  which receptors  and
      contaminants are appropriate subjects of study and how
      best to evaluate exposure at a particular site.  And, as in  all
      other decisions of this  type, the RPM can consult the
      BTAG before committing resources.
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could specify receptors for further study because they are
of concern for statutory  or other reasons (e.g., those
species protected under Federal law). When the RPM has
satisfied these criteria for choosing receptors, he or she can
then consider which of the species are most amenable to
rapid and inexpensive  field evaluation.  Field,  laboratory,
and   literature   studies   conducted   in   the  Problem
Formulation  phase  can   also  aid  in  selecting   and
characterizing receptors. The exposure assessment should
include information on feeding habits,  life history,  and
habitat preferences of receptors.

   To study exposure to contaminants, the work scope
might   include  additional  chemical  analyses  and  the
measurement    or  estimation   of    exposure   point
concentrations.   Chemical  analysis of plant  and  animal
tissues  is one useful technique for  determining  whether
exposure   to   contaminants  has   taken   place.     For
contaminants known to bioaccumulate, analysis of tissues
from organisms representing different trophic levels (e.g.,
plant, herbivore, and carnivore) also permits measurement
of dietary exposure for species that feed on contaminated
organisms.  Biochemical, physiological, and histological
studies can also provide information  about exposure of
receptors to site contaminants.

   The work scope could also specify studying exposure
by means of fate-and-transport models.  Fate concerns the
ultimate chemical disposition of a contaminant,  such as
remaining   stable,  undergoing  photodegradation,   or
combining  with  another   substance.     Transport,  or
migration, refers to the movement of a contaminant from
one  medium to  another, from one location  to another
within  the  same  medium,   or  into  biota.     Site
characteristics,  contaminants'  physical  and chemical
properties,   and   bioaccumulation   studies  provide
information useful in predicting the fate and transport of
site contaminants.

Ecological  Effects Assessment

   Ecological effects  assessment links concentrations of
contaminants to adverse effects in receptors.   Literature
reviews, field studies,  and laboratory studies provide the
information for making this link.  However, the ecological
assessment of a site may not require all three of these types
of studies.

   Field  studies  of  populations  and   communities7
support  ecological  effects  assessment  by  providing
information on  the condition of populations  of  resident
species  and  on  any contaminant-related changes  in
ecological  communities.    In  their  focus on  resident
populations, field  studies play a central role in  identifying
receptors.  Such studies also can allow investigators to
collect samples for laboratory analysis.
   7 A population is a group of organisms belonging to the same species
and inhabiting a contiguous area. A community consists of populations
of different species living together.
   Generally, habitats that are  potentially  or  actually
exposed  to  contaminants  require  some  field  study.
Consulting with the BTAG will enable the RPM to select
the methods and level of effort appropriate to the site and
its remedial objective.  Whenever possible, the work scope
should  specify  standard  or  commonly  accepted  field
methods.  A future ECO  Update will provide information
about field studies useful at Superfund sites.

   Level of effort depends on the choice of qualitative,
semi-quantitative,  or quantitative studies. In some cases,
qualitative studies will adequately describe the habitats and
species at risk.   However, most sites with  suspected
adverse effects  will  require  some semi-quantitative  or
quantitative approach for evaluating effects of stream
pollution  might  sufficiently  characterize differences  in
species   composition   between   contaminated   and
uncontaminated  areas  of stream.  But another site might
require a  more  detailed quantitative analysis  to  discern
such differences.

   An important task in preparing  a work scope involves
coordinating  different types of studies.  In an ecological
effects  assessment,   simultaneous  collection  of  site
chemistry data and biological field data allows the analysis
to show  clearly  whether a correlation exists  between
contaminant presence and ecological effects.

   Toxicity tests  (bioassays) constitute  a major type of
study used in assessing ecological effects at Superfund
sites.  Toxicity tests expose selected organisms to water,
soil,  or sediment from the site to determine whether the
medium adversely  affects the organisms.  Most commonly,
technicians perform  these tests  in  laboratories using
standard test  organisms.  However, toxicity tests also can
occur on-site and can use resident organisms.

   Especially for a  site  with   only   one   or  a  few
contaminants, toxicity tests can contribute to the weight of
evidence linking the  contaminants to biological effects.
Specifically, while chemical analyses indicate the presence
of  contaminants,  they   do    not   indicate  whether
contaminants are  bioavailable.8   In order to have a toxic
effect, a contaminant must be both bioavailable and toxic.
The relationship between toxicity and site contaminants is
less easily interpreted for sites with a more complicated
contaminant picture.

   The work scope should coordinate the collection of site
chemistry data and toxicity data.   When the work scope
specifies that toxicity  tests  will occur in the laboratory,
field  scientists  should   collect  samples  for  chemical
analyses and toxicity tests at the same time and in the same
place.  When the work scope calls for in situ toxicity tests,
chemical sampling should happen concurrently and at the
same locations.  In this way, analysis of the data can most
clearly evaluate  correlations between toxicity results  and
contaminant levels.
   8 Unavailability is the presence of a substance in a form that
organisms can take up.
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                              ECO Update

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   Consulting closely with the BTAG can help the RPM
decide which tests  are  appropriate and  the  specific
conditions under which to conduct the tests. A future ECO
Update will focus  on using toxicity tests in ecological
assessments.

Risk Characterization

   In  ecological   assessments,   risk   characterization
evaluates  the  evidence  linking  site contaminants  with
adverse  ecological  effects.   To characterize  risk,  the
investigator evaluates all of the chemical and biological
data relating  to the  site, comparing the results of the
exposure  assessment  with the results of the ecological
effects assessment.  In particular, fate and transport studies
can provide evidence of links between site contaminants
and observed or predicted effects.

   Also relevant to risk characterization are the results of
the chemical analyses of media, toxicity testing, and field
studies.  At some sites RPMs will have had these studies
conducted along contaminant  gradients.    Where risk
characterization establishes a link between contaminants
and adverse effects, it should also describe the qualitative
or quantitative ecological significance of these effects.

   A successful  work scope  is  one  that  correctly
anticipates the types of studies that will provide  the data
needed for risk characterization.
The results of an ecological assessment
support  the remedial  decision-making
process    only    if    the    data    are
scientifically defensible.
Quality Assurance

   The results of an ecological assessment support the
remedial  decision-making process  only  if the data are
scientifically defensible. Usually, this means that the data
should be (1) accurate and  (2) amenable  to  statistical
analyses (for quantitative studies). Data quality objectives
are qualitative and quantitative statements of the overall
level  of  uncertainly that  a decision-maker is willing to
accept.  Consequently, data quality objectives reflect the
statistical design of the study and the level of significance
needed to support any  conclusion that might be drawn
from the  study.  For example,  the SOW should specify a
sample size large enough to account for natural variability
to ensure that DQOs are met. In reviewing the Work Plan,
the RPM should ensure that minimum sample  sizes are
specified for statistically valid analyses, that significance
criteria meet the needs for remedial decision-making, and
that  quality  control procedures  are  in place to ensure
accuracy and precision.
Before  approving  a  Work  Plan,  the
RPM  should   make   certain   that  the
contractor has  the trained  personnel,
specialized  facilities,  and   specialized
equipment necessary to  carry out the
work.   If not,  qualified subcontractors
should be sought for those tasks where
their qualifications are needed.
   Quality assurance is the set of procedures that ensure
that the quality of data meets the needs of the user. The
Work Plan establishes quality assurance for field work and
laboratory analyses by specifying criteria for such items as
sample  collection, sample  handling, and  numbers  of
replicate analyses. Selecting standard methods specified in
EPA or other federal agency manuals (subject to EPA
approval), when  these  methods  are  appropriate,  can
provide confidence of a stated level of quality assurance
because they have built-in quality control activities.

   Laboratories that conduct standard toxicity tests, such
as those required under the National Pollutant Discharge
Elimination  System  (NPDES),  have in  place  quality
control procedures that are readily subject to review and
audit. Contractors experienced in conducting field studies
should  also  have  standard  procedures for  ensuring
accuracy and reproducibility in their work.  As an example
of quality control in  a field study, a survey of benthic
invertebrates could require an independent taxonomist to
classify a randomly  selected sub-set of the organisms
identified by the study's field or laboratory staff.

   When the work scope specifies clear and  appropriate
quality assurance procedures,  the data collected  should
satisfy  the specified data quality indicators of precision,
accuracy,    representativesness,   completeness,    and
comparability.

Ensuring  Contractor  Capability  To  Do
Work

   Ecological studies trained personnel, and some studies
also require specialized facilities and equipment.  Before
approving a Work Plan, the RPM should be satisfied that
the contractor proposing to carry out the work can do so.
If not, qualified subcontractors should be sought for those
tasks where their qualifications are needed.
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                            ECO Update

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Personnel

   In selecting a contractor, a RPM must look for a direct
match between contractor qualifications and the  scope of
work.  To this end, the RPM should request information on
the  specific   training   and   experience   of  proposed
individuals  with  respect  to  the  specific  tasks  to  be
undertaken.   For  example,  if a  Work  Plan  calls  for
sampling  benthic   invertebrates  in   a  stream,  those
conducting the study should:

   •   Be familiar with the  types  of equipment  (e.g.,
       Surber sampler, artificial substrates) appropriate to
       the study site;

   •   Know how  and where  to  collect samples  (e.g.,
       what kinds of  stream bottoms  support  which
       species);

   •   Know what kinds of environmental data to collect
       along with the  biological  and  chemical  samples
       (e.g., water temperature, pH,  dissolved  oxygen,
       hardness); and

   •   Have the requisite taxonomic expertise to identify
       the  organisms  (principally  the larval stages of
       insects) collected.

   On the other hand,  these same individuals may lack
qualifications for conducting other types of studies, such as
wetland assessments or the collection of small mammals
for tissue  analysis.  Although some experienced biologists
have developed considerable expertise working in a wide
variety of habitats and with a broad range of species, many
others are specialists in their fields and do not know the
details  of  conducting  studies  outside their specialty.
Consequently, the RPM must ask for evidence of specific
individuals' capabilities to carry out proposed tasks.  This
evidence can consist of results of similar studies conducted
in the past.  These results  should demonstrate that the
contractor  performed   studies  correctly  and  that  the
resulting data served its intended purpose.

Facilities and Equipment

   The   RPM   also  should  require  a  contractor  to
demonstrate capability in terms of any specialized facilities
and equipment needed to conduct the studies selected for a
particular site.   For example,  most  of the toxicity tests
used to  evaluate aquatic systems are  standard procedures
developed for NPDES.  Many  States have  certification
programs for laboratories that conduct NPDES  toxicity
tests.  If the work scope calls for such tests at a site, the
RPM  can ask that the contractor use a laboratory certified
in at least one state (if possible, the State where the site is
located), and that the  laboratory  show proof that it has
conducted the same or similar tests  in the recent past.
Alternatively, where a  State and  its neighboring States
have  no certification program, the RPM can obtain the
name  of an appropriate laboratory from the State  agency
charged with regulating NPDES permittees. In the case of
field sampling, the BTAG  or Regional field biologists can
evaluate        a        contractor's        capabilities.
                                      Figure 2. Order Task to an Ecologfeal Assessment
                   An mvesSsf&orcan conduct srnultansausly somsafthe task that t- Waft Plan dstais for
                   w flco'e^e' vssessmement. AS in&pteitbei-?
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   In  all  cases,  contractors must  possess  both  the
appropriate equipment and staff trained on that equipment.
For instance, a commonly used method for collecting  fish
involves electroshock  equipment  that  stuns  the  fish,
causing them  to  float to the  surface.   Electroshock
equipment ranges in size from small backpack units to
large boat-mounted units. For both safety and efficacy, it
is essential to use the right size of equipment manned by a
crew familiar with its operation  and safety requirements.


Review    of    Interim    and     Final
Products

   In keeping with the suggested phased approach,  the
RPM  should plan for BTAG  review of interim products
such as initial site description, initial field surveys,  and
reports on specific studies such as the basis for revising the
work scope to account for the new findings.

   In addition to the interim  products mentioned above,
the RPM should have the BTAG review the  draft Work
Plan  and the  draft ecological  assessment  before  the
contractor proceeds with the final version. With regard to
the  draft  ecological   assessment,  the  RPM  should
particularly request the BTAG to  comment on the quality
of studies and the validity of their findings. The  RPM  will
also want to  know  whether the  data  support  any
conclusions about proposed remedial actions at the site.


Sample Work Scope

   The  Appendix  presents  an example of the  kinds of
components likely  to occur in a typical  work scope.  Of
course, work scopes designed for particular sites will differ
significantly from the general one in  the Appendix.  An
RPM will find it necessary to  tailor the work scope to the
specific conditions and objectives at an individual site.

   The  example in the Appendix also demonstrates how
BTAG review of interim products can alter the scope and
level of effort for succeeding tasks. The example always
states that products are subject to review and approval by
the site  manager,  because the BTAG  has  no  official
authority  to  approve  or  disapprove contractor  work.
Nevertheless, wherever appropriate, the  RPM should  ask
the BTAG for review  and  advice  on each product.   In
scheduling a project, RPMs need to  allow time for  the
review process.  In fact, some Regional BTAGs require a
minimum review period.

   In addition to the general work scope in the Appendix,
RPMs in several Regions have available to them generic
work  statements or other guidance material prepared by
their BTAGs.    RPMs  should  check with  the  BTAG
coordinator in their Region to obtain any such guidance.


Conclusion

   This Bulletin has  summarized the issues  and RPM
needs to  address in  developing work  scopes for the
ecological assessment  of Superfund sites.  Because every
site presents a  unique combination of study  problems,
RPMs  should  consider  the  expert  advice  of  BTAG
members as an essential part of the planning process for
these assessments.  These specialists should be consulted
as early as possible in the planning stages for  a site, and
should remain  involved in the  planning and oversight
throughout the life of the project.  By involving the BTAG
in this way, the RPM can be assured that ecological as well
as human health effects  will receive the  full attention
called for in the law and in Agency policy directives.
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                             ECO Update

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                                                  APPENDIX
                 SUGGESTED TASKS IN PLANNING AND EXECUTING AN

                                     ECOLOGICAL ASSESSMENT

      The following tasks can help a contractor in assembling an acceptably detailed and focused ecological assessment.  Wherever
possible, these tasks should be coordinated with the human health assessment and any hydrogeologic investigations.
      A site's ecological assessment may not require  all of the  tasks.   For example,  with  site  description  (Task  1) and  the
reconnaissance visit (Task 2) complete, the RPM  may decide that the Work Plan can be drafted (Task 4) without any further site
characterization (Task 3).
      Note also that an investigator can conduct certain tasks simultaneously rather than sequentially, greatly enhancing the efficiency
of the process (Figure 2).  Precisely which tasks can occur simultaneously and which the investigator must conduct sequentially
depend upon the site.
Task 1. Site Description
   Purpose:      Preliminary screening of the extent of contamination and the potential effects.
   Description:  Qualitatively describe site based on existing data from the Preliminary Assessment,  Site Inspection,  and other
                sources, including:
                1.  Physical description of the site and its surroundings, including photos  and detailed maps;
                2.  Nature and extent of contamination by medium and contaminant type;
                3.  Site-associated habitats potentially exposed to contaminants; and
                4.  Initial toxicity assessment of site contaminants with respect to environmental receptors, including comparison
                   to criteria and other benchmarks.
   Submit interim report to site manager for review.

Task 2. Site Reconnaissance Visit
   Purpose:      Gather first-hand expert opinion of site's condition and suggestions about what, if any, studies are needed.
   Description:  If authorized by site manager, prepare plan for site reconnaissance, including:
                1.  Chemical and biological data needed for more complete initial site description;
                2.  Methods to be used to collect necessary data; and
                3.  Criteria for deciding whether and what future studies might be necessary.
   Submit reconnaissance plan to site manager for review.
Task 3. Site Screening
   Purpose:      With limited studies, identify and characterize habitats and characterize exposure and ecological effects.  [For some
                sites,  information will suffice for risk characterization.]
   Description:  If authorized by site manager, further characterize sie based on field observations, including, as appropriate:
                1.  More detailed habitat identification and evaluation;
                 a.   Suitability for wildlife, including an endangered species consultation with State and Federal agencies
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                 b.  Ecosystem value and function (e.g., wetland functional analysis)
                2.   Qualitative and semi-quantitative surveys of flora and fauna;
                3.   Toxicity tests;
                4.   Additional chemical sampling;
                5.   Identification of appropriate references sites for comparison to each potentially exposed habitat; and
                6.   Simple modeling of transport and exposure.
    Submit interim report to site manger for review.

Task 4. Draft of Work Plan
    Purpose:     Develop a plan that will provide any additional information about exposure and ecological effects that is needed to
                characterize risk.
    Description:  Draft detailed Work Plan for any  further site investigations needed, including overall assessment objective and, as
                appropriate:
                1.   Qualitative, semi-quantitative, and quantitative surveys of flora and fauna in potentially exposed habitats and
                     reference sites;
                2.   Chemical sampling of media and biota in potentially exposed habitats and reference sites;
                3.   Laboratory and in situ toxicity testing;
                4.   Tissue analyses, enzyme studies, and bioaccumulation studies; and
                5.   Simple modeling of fate and transport.
                For each proposed study above, provide:
                a.   Objectives of the study, effects to be measured, and relevance to overall risk assessment objectives at the site;
                b.   Proposed field or laboratory methods and their risk-based detection limits (where appropriate), with appropriate
                     references to Agency guidelines or other source;
                c.   Criteria for determining sampling locations, expected sampling locations (including detailed maps),  sampling
                     dates, and sample sizes;
                d.   Benchmark, or background values, where appropriate;
                e.   Statistical methods to be used and data quality indicators to meet statistical significance criteria; and
                f.    Quality assurance procedures and quality control techniques.
    Submit Work Plan to site manager for review and approval. Revise per site manager's direction.

Task 5. Data Collection
    Purpose:     Gather necessary data regarding exposure and ecological effects
    Description:  Conduct those studies approved by site manager for immediate execution.  Submit interim reports to site manager
                for review.
 Task 6. Final Data Collection
    Purpose:     Based on findings of studies conducted, identify and collect any final data needed to assess exposure and ecological
                effects.
    Description:  Revise Work Plan per site manager's direction. Conduct next phase of studies as approved by site manager.  Submit
                interim reports to site manager for review.  Repeat this step as needed.  Task  6 is an iterative process that will
                lengthen or shorten, depending on the results of studies.
Task 7. Risk Characterization
    Purpose:     Validate the data and their interpretations, and characterize risk.
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   Description:  Prepare the following for review by site manager:
                 1.   Summary of biological and chemical data; and
                 2.   Detailed outline of ecological assessment.

Task 8. Report Preparation
   Purpose:      Prepare data for presentation.
   Description:  Prepare draft ecological assessment.
   NOTE:       Depending on the scope and level of effort decided on by the site manager, not all of the elements listed below may
                 appear in a given assessment. For instance, not all sites will require toxicity testing or the full array of quantitative
                 field studies. The following outline should be modified to account for the studies actually undertaken at the site
                 with the approval of the site manager.
                 1.   Initial site description and potential receptors (include detailed maps wherever appropriate)
                  a.   Physical description of the site
                  b.   Nature and extent of contamination by medium and contaminant type
                  c.   Potentially exposed habitats
                   (i)      Surface water habitats
                   (ii)      Wetlands
                   (iii)     Terrestrial habitats
                   (iv)     Sensitive or critical habitats
                  d.   Potentially exposed species
                   (i)      Vegetation
                   (ii)      Invertebrates
                   (iii)     Vertebrates
                   (iv)     Special concern species
                 2.   Selection of contaminants, species, and ecological effects of concern
                  a.   Contaminants of concern and rationale for selection
                  b.   Species of concern and rationale for selection
                  c.   Ecological effects of concern,  acceptable and unacceptable levels of effects, temporal and spatial scales or
                      concern, and rationale for selection
                 3.   Exposure assessment
                  a.   Sources and exposure pathways of contaminants of concern
                  b.   Fate and transport analysis
                  c.   Exposure scenarios
                  d.   Estimated exposure point concentrations by habitat, species and exposure scenario
                  e.   Uncertainty analysis
                 4.   Ecological  effects assessment
                  a.   Known effects of contaminants of concern (from literature)
                  b.   Site-specific toxicity tests—laboratory and in situ
                  c.   Existing toxicity-based criteria and standards
                  d.   Uncertainly analysis
                 5.   Risk Characterization
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                  a.   Observed adverse effects in potentially exposed habitats compared to reference sites
                   (i)      Mortality and morbidity
                   (ii)      Vegetation stress
                   (iii)     Habitat degradation
                   (iv)     Presence or absence of key species
                   (v)      Population assessment of key species
                   (vi)     Community indices
                   (vii)     Ecosystem function, such as decomposition or nutrient recycling
                  b.   Analysis of contaminant concentrations in relation to observed adverse effects
                  c.   Analysis of bioaccumulation studies
                  d.   Analysis of toxicity test results in relation to observed adverse effects
                  e.   Comparison of estimated exposure point concentrations with criteria and standards
                  f   Comparison of  estimated exposure  point  concentrations with  toxicity  data  and/or toxicity values from
                      literature, as appropriate
                  g.   Likely ecological risks associated with present and future land use scenarios
                  h.   Ecologically relevant ARARs
                  i.   Ecological consideration in selecting remedial alternatives (including no action)
                  j.   Uncertainly analysis
    Submit draft ecological assessments to site manager for review.

Task 9. Report Revision
    Purpose:     Prepare final presentation of ecological assessment.
    Description:  Revise draft ecological assessment per site manager's review comments and submit final ecological assessment for
                inclusion in RI/FS.
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