v»EPA United States Environmental Proloction Agency EPA 910-R-15-002 U.S. Environmental Protection Agency Regional Laboratory Network Annual Report 2014 ------- Cover: Snapshot of environmental laboratory science themes of Protecting Water; Addressing Climate Change; Improving Air Quality, Toxics and Chemical Safety, Communities; State, Tribal, and Local Partnerships; High-Performing Organization; and Sustainable Future. Cover photos were obtained from EPA Office of Multimedia intranet site, except for two images listed below. Photo: Water Lily Photographer: Steve Donohue/EPA Photo: Trees Photographer: Randy Pomponio//EPA ------- FY2014 Annual Report Table of Contents Page Executive Summary ii Section I U.S. EPA Regional Laboratories/Regional Laboratory Network: An Overview 1 U.S. EPA Regional Laboratories 2 Regional Laboratory Network: An Overview 4 Section II Regional Laboratory Network Highlights 7 Protecting Water: A Precious, Limited Resource 8 Addressing Climate Change and Improving Air Quality 12 Taking Action on Toxics and Chemical Safety 14 Making a Visible Difference in Communities across the Country 16 Launching a New Era of State, Tribal, and Local Partnerships 18 Embracing EPA as a High-Performing Organization 19 Working toward a Sustainable Future 23 Section III FY2014 Laboratory Accomplishment Results Summary 25 Section IV Regional Laboratories Capabilities Tables FY2014 29 Regional Laboratories Core Capabilities FY2014 30 Regional Laboratories Unique Capabilities FY2014 33 Regional Laboratories Developing Capabilities FY2014 43 Appendix A Acronyms/Abbreviations A-1 J List of Illustrations Figure 1. Analytical Support to EPA Programs in FY2014 2. FY2014 U.S. EPA Regional Laboratories Analyses Provided by Program 3. Projects/Sites Supported by Regional Laboratory Data FY2014 by Program Element 4. Method Development Project Support to EPA Programs in FY2014 Page 26 26 27 27 ------- FY2014 Annual Report Executive Summary The U.S. Environmental Protection Agency (U.S. EPA) Regional Laboratories are state-of-the-art, full-service environmental laboratories delivering analytical services, field support, expert quality assurance and data review, and innovative technical assistance. Laboratory personnel are an integral part of the science backbone of the Agency. Their skills and expertise allow the development and implementation of solutions to complex issues affecting human health and the environment. Ten Regional Laboratories individually support the 10 regions within the EPA while also collaborating to form a highly effective Regional Laboratory Network (RLN). This report highlights the diversity of support and capabilities, all of which reinforce EPA's mission and ongoing priorities. In Fiscal Year 2014 (FY2014), Regional Laboratories performed over 143,000 analyses in support towards agency priorities and solving emerging environmental issues. This analytical work supported activities related to over 1,500 sites and projects associated with a wide range of agency programs. To effectively support the EPA Administrator's priorities and themes, Regional Laboratories demonstrated their responsiveness and flexibility by pioneering citizen science programs to enhance sound science within communities across the nation, researching the effects of toxins on the natural environment and emerging threats to human health, modifying methodologies to meet the demand of lower risk levels or detection of unknown threats, responding to emergencies, and providing defensible data. Real-time data continued to be an important tool for environmental decision-making. Regional Laboratories have a wide-range of field analytical support services ranging from on-site analysis in mobile laboratories to screening techniques performed directly in the field. In FY2014, Regional Laboratories performed over 4,000 field analyses in support of a combination of 200 projects and sites throughout the country. Accomplishments presented in this report capture only a few of the overall activities provided by all Regional Laboratories. These accomplishments underscore the commitment of the RLN to be an integral part in protecting human health and the environment. FY2014 Annual Report Structure Section I. U.S. EPA Regional Laboratories/ Regional Laboratory Network: An Overview Provides introduction and overview of the Regional Laboratory System. This section also contains method development/research collaboration information to enhance science. Section II. Regional Laboratory Network Highlights Describes accomplishments of the Regional Laboratories as they relate to EPA Administrator's Themes. Section III. FY2014 Laboratory Accomplishmer Results Summary Summarizes RLN common support services with corresponding charts. Section IV. Regional Laboratories Capabilities Tables FY2014 Displays the abilities of Regional Laboratories to conduct certain tests within chemistry, physical and other determinations, and biology. EPA Agency Themes Meeting the Challenge Ahead Protecting Water: A Precious, Limited Resource Addressing Climate Change and Improving Air Quality Taking Action on Toxics and Chemical Safety Making a Visible Difference in Communities across the Country Launching a New Era of State, Tribal, and Local Partnerships Embracing EPA as a High-Performing Organization Working toward a Sustainable Future I EPA 910-R-15-002 ------- FY2014 Annual Report Section I U.S. EPA Regional Laboratories/Regional Laboratory Network: An Overview &EPA United States Environmental Protection Agency EPA 910-R-15-002 ------- FY2014 Annual Report U.S. EPA Regional Laboratories Region 1: New England Regional Laboratory Investigation & Analysis Branch Ernest Waterman, Director Waterman.Ernest@epa.gov 11 Technology Drive N. Chelmsford, MA 01863-2431 Phone:617-918-8632 FAX: 617-918-8540 Region 2: Division of Environmental Science and Assessment Laboratory Branch John Bourbon, Director bourbon.iohn@epa.gov 2890 Woodbridge Ave. Edison, NJ 08837 Phone:732-321-6706 Fax:732-321-6165 Region 3: Environmental Science Center Laboratory Branch Karen Costa, Manager Costa.Karen@epa.gov 701 Mapes Road Ft. Meade, MD 20755-5350 Phone:410-305-2689 Fax:410-305-3095 Region 4: Analytical Support Branch Danny France, Director France.Danny@epa.gov 980 College Station Road Athens, GA 30605-2720 Phone: 706-355-8551 Fax: 706-355-8803 Region 5: U.S. EPA Region 5 Laboratory, Chicago Regional Laboratory Dennis Wesolowski, Director wesolowski.dennis@epa.gov 536 S. Clark Street Chicago, IL 60605 Phone:312-353-9084 Fax:312-886-2591 ------- FY2014 Annual Report U.S. EPA Regional Laboratories Region 6: Environmental Services Branch Wes McQuiddy, Director Mcquiddv.David@epa.gov 10625 Fallstone Road Houston, TX 77099 Phone:214-665-6722 Fax:281-983-2124 Region 7: Regional Science & Technology Center Margie St. Germain, Director Stgermain.margie@epa.gov 300 Minnesota Ave. Kansas City, KS66101 Phone:913-551-5154 Fax:913-551-7873 Region 8: U.S. EPA Region 8 Laboratory Mark Burkhardt, Director Burkhardt.Mark@epa.gov 16194 West 45th Drive Golden, CO 80403 Phone:303-312-7799 Fax:303-312-7800 Region 9: U.S. EPA Region 9 Laboratory Duane James, Acting Director James.Duane@epa.gov 1337S. 46th Street, Bldg. 201 Richmond, CA 94804-4698 Phone:510-412-2300 Fax:510-412-2302 Region 10: Manchester Environmental Laboratory Barry Pepich, Director Pepich.Barry@epa.gov 7411 Beach Drive East Port Orchard, WA 98366 Phone:360-871-8701 Fax:360-871-8747 ------- FY2014 Annual Report Regional Laboratory Network: An Overview EPA has 10 regional offices and each region has a laboratory. The Regional Laboratories provide mission-critical support to the Agency, protecting human health and the environment. Service and expertise provided by each Regional Laboratory are tailored to meet the needs of that particular region or program and to address complex and emerging environmental issues. In addition to supporting each region, the 10 Regional Laboratories collaborate to form the Regional Laboratory Network (RLN). Efficiency, effectiveness, and flexibility are maximized by using scientific expertise, implementing and developing methods, and maximizing partnerships within RLN and across the nation. Services tailored to meet regional needs and to address complex and emerging environmental issues Environmental decisions and policies provide the RLN with the analytical structure to meet program needs. Regional Laboratories also provide support to national initiatives and research. Each Support special project- specific objectives and goals towards a sustainable future laboratory within the RLN constantly and consistently meets and supports project-specific objectives, achieves quality goals, provides analytical expertise, and produces accurate data within the Agency. Accreditation following National Environmental Laboratory Accreditation Conference (NELAC) or International Standards Organization (ISO) 17025 EPA Regional Laboratories are committed to producing quality data. The laboratories follow EPA organizational directives for a high-performing organization. All 10 laboratories are accredited by National or International Accreditation programs ensuring effective quality systems, improved performance, and defensible data. External assessments are performed regularly at RLN laboratories. Contracting mechanisms are used within the RLN to provide additional procurement of analytical services. The Contract Laboratory Program (CLP) provides standard analytical methods supporting the Superfund Program. Each laboratory uses an Environmental Services Assistance Team (ESAT), which is a contract to support laboratory functions. This organizational structure permits EPA Regional Laboratories to provide quick response to emergencies, while providing timely completion of all projects. During FY2014, 10 Regional Laboratories supported over 143,000 sample analyses and over 1,500 projects. RLN supports Agency quick responses to emergencies ------- FY2014 Annual Report Regional Laboratory Network: An Overview Regional Laboratory scientists are a valuable resource. Scientists have expertise in analytical methods, quality assurance and quality control principles, data validation, field analytical techniques, and solving complex analytical projects. Support analytical During FY2014, the 10 Regional method improvements Laboratories supported more than 125 method improvement projects. Regional Laboratory scientists are certification officers for the Drinking Water Laboratory Certification Program and participate in state drinking water audit programs. Laboratory scientists also provide ^^^^^^^^^^^^^^ management, technical, logistical, and oversight support to EPA, State and tribal programs, operate air monitoring quality assurance programs, and support field sampling functions. Serve crucial roles in regional drinking water audit programs EPA Regional Laboratories provided analytical responses to approximately 15 significant emergency response events in FY2014. EPA Regional Laboratories are capable of analyzing samples suspected to contain a variety of chemical and biological compounds, including chemical warfare agents. Also, some Regional Laboratories developed and validated new methods for chemical warfare agent degradation compounds that characterize and remediate contaminated areas. Respond to Homeland Security events Within each Regional Laboratory, core capabilities allow support to various EPA programs. Unique capabilities provide the flexibility for each laboratory to meet geographical environmental demands or regional and national initiatives. Three tables (core, unique, and developing) summarize chemical, physical and biological/ microbiological capabilities for each region. The Core, Unique, and Developing capabilities tables for each Regional Laboratory are provided in Section IV and are available on the following EPA websites. Core, Unique, and Developing capabilities span across RLN Regional Laboratories Core Capabilities FY2014 http://www.epa.qov/aboutepa/reqional-science-and-technoloqy-lab-core-capabilities Regional Laboratories Unique Capabilities FY2014 http://www.epa.gov/regionallabs/epa-regional-laboratories-unique-analvtical-capabilities-and-documentation-region Regional Laboratories Developing Capabilities FY2014 http://www.epa.gov/measurements/collection-methods ------- ------- FY2014 Annual Report Section II Regional Laboratory Network Highlights xvEPA United States Environmental Prelection Agoncy EPA 910-R-15-002 ------- FY2014 Annual Report Protecting Water: A Precious, Limited Resource Ensure drinking water is safe. Restore and maintain oceans, watersheds, and their aquatic ecosystems to protect human health, support economic and recreational activities, and provide healthy habitat for fish, plants, and wildlife. Regional Laboratories play an important part in protecting and restoring the nation's water resources by providing: key data for regions and their partners and target actions to protect human health and aquatic ecosystems more efficiently technical and regulatory support to drinking water laboratories and training and support for water quality monitoring efforts analytical support for various projects across the U.S. Drinking Water Laboratory Certification Laboratories that analyze drinking water samples are required to be certified by an approved certifying authority. The Environmental Protection Agency (EPA) Regional Laboratory personnel, trained certification officers, conduct on-site evaluation of drinking water laboratories operated by states and tribal communities. Certification Officers also conduct audits of state's certification programs to ensure all laboratories analyzing drinking water samples are following approved methods mandated by EPA's National Primary Drinking Water Regulations. Ultimately, the effort of the laboratory certification program ensures drinking water is free from harmful contaminants. In FY2014, Regional Laboratories performed 31 evaluations and 14 audits. Regional Laboratories supported water-related projects, including TMDL Studies Water Quality Assessment and Total Maximum Daily Load (TMDL) Program Support Crucial for management of our water resources, water quality data are used to characterize waters, identify trends, identify emerging problems, determine whether pollution control programs are working, and help direct pollution control efforts to where they are most needed. A TMDL is a calculation of the maximum amount of a pollutant that a waterbody can receive and still meet water quality standards, and an allocation of that load among the various sources of that pollutant. Regional Laboratories provide substantial analytical support for water quality assessments to, and TMDL development for, water bodies throughout the country. Methyl Mercury TMDL for San Francisco Bay Delta Watershed Region 9 Laboratory used its recently-developed capability to support a study of methylmercury in the San Francisco Bay Delta Watershed. As one of the largest and most important estuarine systems on the West Coast, the Bay Delta estuary is supplying drinking water to 25 million people and irrigation water for 4 million acres of farmland. Methylmercury, a potent neurotoxin, poses a unique water management challenge in the Bay Delta. After establishing a TMDL, Region 9 Laboratory, in collaboration with a joint project with the Central Valley Regional Water Quality Control Board, analyzed multiple sets of water samples from agricultural sites. The data provided will be used to develop a more accurate baseline of methylmercury loads associated with irrigated agriculture, thereby refining TMDL levels. EPA 910-R-15-002 ------- FY2014 Annual Report rotecting Water: A Precious, Limited Resource TMDL Studies in Springfield, Missouri The Wilson, Jordan, and Pearson Creek TMDLs were established January 2011 after named as impaired waters from multiple-point sources and urban nonpoint sources. The pollutant causing the impairment is listed as unknown; however, toxicity from multiple pollutants and changes in hydrology from increased impervious surfaces are the suspected cause of the impairment. By establishing these TMDLs, EPA met the milestones of the 2001 Consent Decree. However, after a ruling in 2011, EPA was required to further consider impacts based on additional water quality. To meet a settlement agreement, Region 7 conducted monthly water and sediment sampling and diurnal dissolved oxygen monitoring. Water grab samples were analyzed for total phosphorus, total nitrogen, suspended solids, dissolved solids, total solids, dissolved metals, and hardness. Sediment grab samples consisted of metals and polycyclic aromatic hydrocarbons (PAHs). Water and sediment samples from urban streams in Springfield were collected for over 10 different traditional methods, including metals, poly-aromatic hydrocarbons, anions, hardness, semi-volatile organics, and pH. This effort supports EPA's interest in determining what pollutants may be present and whether the creeks meet Missouri water quality standards and effectively restore these streams. The data will be used to write pollutant-specific TMDLs for each creek. i For more information: (American Canoe Association, et al. v. EPA, No. 98-1195-CV-Win consolidation with No. 98-4282- CV-W, February 27, 2001). (City of Springfield vs. EPA, etal., (6:11-cv-03383) Wilson, Jordon and Pearson Creeks). Field Auditing for National Rivers and Streams Assessment Region 9 Laboratory provided field auditing support for the National Rivers and Streams Assessment (NRSA) 2013-2014. The national survey is designed to estimate the percentage of rivers and streams in good, fair, or poor condition, help citizens and water quality managers evaluate the effectiveness of protection and restoration efforts, and take action to prevent pollution. Trained sampling crews, consisting of representatives from states, tribes, interstate agencies, and private contracts collected samples at 1,800 sites across the country using the same methods at each site. Sampling crews took numerous measurements, including: temperature, dissolved oxygen, nutrients, pH, and more habitat condition along river or stream banks algae and free-floating plants benthic macroinvertebrates - small aquatic animals: insects, snails, and crayfish (a source of food for fish) bacteria indicators of possible fecal contamination type and abundance offish contaminants in fish tissue. I Regional Laboratories supported HHi Oversight- or Enforcement-related activities Environmental Studies for Ocean-Dredged Material Management of dredged material and Ocean-Dredged Material Disposal Site (ODMDS) is a shared responsibility of EPA and the U.S. Army Corps of Engineers (USAGE) under the Clean Water Act (CWA) and Marine Protection, Research, and Sanctuaries Act (MPRSA). Each year, USAGE Portland District (CENWP) dredges 3-to-5 million cubic yards of sediment at the Mouth of the Columbia River (MCR) to maintain the 6-mile long, deep-draft, navigation entrance channel. MCR-dredged material is placed at two EPA-designated ODMDS locations. In 2014, Region 10 Laboratory participated in studies designed to assess the status of the physical, chemical, and biological environment on benthos within previous, current, and future drop zones. EPA 910-R-15-002 ------- FY2014 Annual Report Protecting Water: A Precious, Limited Resource Effectiveness of Storm Water Best Management Practice (BMP) Evaluated Since its establishment, EPA made it a goal to adhere to BMPs to protect the quality of water and soil. Following BMP guidelines is particularly important when managing the impacts of nutrient loading that builds within storm water runoff and enters receiving waters. With constantly advancing technology, EPA must respond to changing times and create new ways to continue protecting the environment in the most efficient way possible. To ensure the most current standards, Region 1 Laboratory partnered with the regional water program and two municipalities on a study to evaluate effectiveness of regional storm water BMPs. Partners conducted a project to monitor and evaluate the BMPs that will continue for a minimum of 2 years. The ultimate goal of this project is to determine the most effective and practical set of BMP guidelines to reduce nutrient loads flowing into receiving waters. For this project, groups worked together to: 1. Identify key water constituents to be analyzed by Region 1 Laboratory and establish general water quality parameters to asses performance of BMPs in treating storm water. 2. Develop a basic Quality Assurance Project Plan (QAPP) for use during BMP construction phases. The QAPP will be used to test sample access methods and storm water content. Afterwards, a second, more-comprehensive QAPP will be implemented to carry sampling and analysis of BMP performances from post-installation through a minimum of 20 rain events and meeting project data quality objectives. 3. Develop performance rating curves for each BMP and create a set of efficient and cost-effective BMP guidelines. The top BMP arrangements chosen will be extremely valuable in improving EPA's storm water management. The BMPs selected are expected to help with concerns, such as mitigating the costs of development and implementation. The resulting impact of BMP guidelines will be widespread. Pharmaceuticals and Personal Care Products fPPCP) from Hospital Discharges Region 2 Laboratory coordinated a 4-month collaborative study to characterize PPCP in hospital effluent and sewage treatment plant (STP) influent/ effluent. The study involved six hospitals discharging into four STPs in Suffolk County, New York. Suffolk County field personnel collected the samples from the six hospitals and four STPs. Region 2 Laboratory, in collaboration with Region 8 Laboratory, conducted PPCP analysis of 72 samples, providing results for 206 compounds. Results were used by the Division of Enforcement and Compliance Assistance (DECA) in June 2014. Regional Laboratories All provide scientific expertise to support Regional and National Water Programs and Initiatives, which can include analysis, field support, quality assurance and data review, and technical support. EPA 910-R-15-002 ------- FY2014 Annual Report Protecting Water: A Precious, Limited Resource Cvanobacteria Monitoring & Bloom Watch Pilot Program Cyanobacteria and their associated toxins are of particular concern to humans and the ecology. Increasing precipitation and land development affects nutrient loads to aquatic systems. Impacted water systems are shut down when cyanotoxins are in the water supply. Ingestion of these algal toxins affects people, animals, wildlife, and pets. In New England, regional shifts in monitoring efforts highlighted varying cyanobacteria concentrations and toxicity levels. However, different sampling approaches and methodology inconsistencies made it extremely difficult to aggregate data, understand algae characteristics and behavior, and share information across the region. In 2012, the Region 1 Laboratory convened a region- wide (including New York) cyanobacteria monitoring and "bloom watch" workgroup to ensure uniform consistency with cyanobacteria monitoring methods and protocols. The workgroup consisted of state environmental water quality and beach monitoring programs, departments of public health, tribes, public water suppliers, Non-Governmental Organizations (NGOs), citizen monitoring groups, and academia. In May 2014, the workgroup established a pilot monitoring project, which included an algal taxonomic identification (ID) workshop, methods training, and instrument calibration protocols. Sampling commenced in June 2014 in all New England states. Three principal monitoring components: were established: in-lake monitoring, shore-side monitoring, and a qualitative "bloom watch" component. In-lake monitoring consisted of a minimum of three sampling sites with collection of an integrated water sample, a cross-section from the surface to 3 meters at each site and a filtrate sample. Shore-side monitoring consisted of a minimum of one sample, a 1-meter integrated sample and one filtrate sample. The site locations remain consistent; additional sites can be added at the discretion of the sampler. Protocols allow samples to be analyzed on-site using a handheld fluorimeterto measure chlorophyll and phycocyanin, or frozen to be analyzed at a debriefing session. Fluorimeters were provided to the New England states through the Region 1 Water Monitoring Equipment Loan Program. The "bloom watch" component established protocols to document time and location of a perceived algal bloom and track the frequency and spatial occurrence in the region. Site photos, can be enhanced at microscopic levels for algal identification purposes. A demonstration was provided showing how a smartphone can mimic an inexpensive field microscope. Smartphone apps have been developed and are being tested. A regional geo-referenced database capable of providing "vulnerability assessment" of a waterbody based on hydro geomorphic characteristics and land use patterns in the watershed is also being tested. Upon project completion, the workgroup will evaluate their progress, refine the approach, make recommendations, and develop a monitoring program. Capability to analyze water samples for over EPA and other standardized methods EPA 910-R-15-002 ------- FY2014 Annual Report Addressing Climate Change and Improving Air Quality Protect and improve the air so it is healthy to breathe and risks to human health and the environment are reduced. Reduce greenhouse gas intensity by enhancing partnerships with businesses and other sectors and common-sense regulatory initiatives. Regional Laboratories actively support the objectives of the Agency's air goals through a variety of activities. These activities include: technical support and training air monitoring and air monitoring quality assurance laboratory support for various air toxics assessments laboratory support for numerous other local projects that address specific community risks and method development. Sorbent Tube Analysis of Routine Air Samples Provide Cost-Effective Alternative Typically, air samples for volatile organics are collected in Summa canisters following Method TO-15 from the EPA Compendium of Methods for the Determination of Toxic Organic Compounds in Ambient Air. Region 7 developed an enhanced method using sorbent tubes similar to Method TO-17. A three-phased sorbent tube that collects air samples of low to moderate humidity was field-tested at three different sites. Results were comparable to Method TO-15 and met Method TO-17 requirements. Future method development includes validating a single-phase sorbent tube that will also produce good results in high humidity. (Q) Regional Laboratories have the capability to analyze for Organic Compounds in Air Vapor Intrusion Study in West Virginia Vapor intrusion occurs when volatile chemicals migrate from contaminated groundwater or soils into an overlying building in ways similar to that of radon gas seeping into homes. When remediating a contaminated site, analysis of indoor air may be required to assess presence of vapor intrusion. Typically, EPA Method TO-15 using air canisters is employed to measure level of possible contamination. In 2014, an extensive vapor intrusion sampling event was conducted at a West Virginia remedial site to determine if a new vapor plume emanated from an underlying tetrachloroethylene (PCE) plume. The study was designed to assess impacts to local residents living above the plume and Region 3 Laboratory provided air analysis on these samples. Pictured above is an air canister used for volatile organic analysis of air samples. EPA 910-R-15-002 ------- FY2014 Annual Report Addressing Climate Change and Improving Air Quality PM 2.5 Performance Evaluation Program (PEP) The goal of the PEP is to evaluate total measurement system bias of the particulate matter (PM) 2.5 monitoring network. The laboratory component of the program includes PM filter handling, inspection, equilibration, and weighing; data entry, validation, management, and distribution to client regions; and filter archival and data submittal to the Air Quality System (AQS). The PM filter weighing laboratory is located at Region 4. Other Regional Laboratories also provided support for PEP through performance evaluation audits, quality I assurance collocations, and PEP audits. Regional Laboratory staff provided training classes for the PM 2.5 PEP program. Ambient Air Audits at A/Core Stations NCore is a national multi-pollutant network that integrates several advanced measurement systems for particles, pollutant gases, and meteorology. NCore air monitoring sites operate trace-level instruments that are difficult to audit with conventional through-the-probe air monitoring equipment. Region 2 developed a standard operating procedure (SOP), used nationally, for conducting these audits, and has identified inaccuracies and potential sources of error. Currently, the region is assessing the scope and magnitude of errors/inaccuracies in existing procedures and methods, and is testing alternative calibration and/ or analytical strategies for NCore audits. PM 2.5 audits conducted in FY2014 Air Quality Monitoring Requires Extensive Analysis In Pennsylvania, a proposed natural gas production project was identified to measure baseline air quality. The proposed plan outlined locations to conduct ambient air sampling for volatile organic compounds (VOCs). The monitoring project was part of the State and Local Agency Monitoring Stations (SLAMS), which consists of a network of monitoring stations. Size and distribution is determined by the needs of state and local air pollution control agencies to meet their respective state implementation plan (SIP) requirements. The primary purpose of air monitoring is to determine if concentrations of certain VOCs can be shown to statistically correlate to gas exploration and production activities. Air samples were collected for a period of up to 18 months and will continue the following year after gas exploration begins. Sampling began in February 2014 and analysis was conducted by the Region 3 Laboratory using EPA Method TO-15. EPA 910-R-15-002 ------- FY2014 Annual Report Taking Action on Toxics and Chemical Safety Keeping communities safe and healthy by reducing risks associated with exposure to chemicals in commerce, indoor and outdoor environments, and products and food. The Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA), or Superfund, and the Resource Conservation and Recovery Act (RCRA) provide the legal basis for EPA's efforts to preserve and restore land using the most effective waste management and cleanup methods available. By supporting these programs, the Regional Laboratories: Analyze hazardous and non-hazardous waste Implement applied research and method development to meet evolving analytical needs of Superfund and RCRA programs Ensure vigilant readiness for emergency response to environmental disasters, hazardous materials releases, time-critical removals, and inland oil spills. Perfluorinated Chemicals fPFCs) Method Evaluated for Wastewater and Solid Matrices PFCs are an ongoing concern for EPA. Products manufactured and treated with PFCs include clothing, fabrics, surface treatments, cleansers, and cookware, which enter the environment via down-the-drain disposal. Other PFC products are directly discharged into the environment from fire-fighting foams and surface treatments. Region 5 Laboratory participated in preliminary semi-continuous activated sludge (SCAS) experiments and evaluated other PFC tests. An analytical method to measure PFCs in wastewater and solid matrices was developed and accepted by the American Society for Testing and Materials (ASTM), analyzing PFCs for SCAS study by an approved method. Trichloroethvlene (TCE) Impacts Investigated in Meramec Caverns The topography of southeastern Missouri is characterized by numerous caves, sinkholes, fissures, and underground streams. Meramec Caverns is one of the largest cave systems in Missouri. Unfortunately, it is impacted by a subsurface plume of TCE that originates from a site approximately 5 miles away. Concerns were raised about TCE exposures to visitors and employees touring the commercialized portion of Meramec Caverns and the ecological health of cave- dwelling fauna. Using Region 7 mobile laboratory, on-site TCE concentrations in both commercialized and native portions of Meramec Caverns were analyzed by gas chromatography/mass spectrometry (GC/MS) on samples of cave air using portable bag samplers. The data generated characterized distribution, concentration, and ventilation of TCE throughout the cave complex. This project is continuing into FY2015. )f Regional Laboratory analyses support Superfund program EPA 910-R-15-002 ------- FY2014 Annual Report Taking Action on Toxics and Chemical Safety Ongoing Monitoring at Wvckoff/Eagle Harbor Superfund Site The Wyckoff/Eagle Harbor Superfund site is located on the east side of Bainbridge Island in Central Puget Sound, Washington. The East Harbor Operable Unit (OU) 01 consists of more than 70 acres of intertidal and subtidal habitats contaminated by releases of creosote and other wood-treating chemicals from a now defunct wood-treating plant. The releases contaminated bottom sediments of Eagle Harbor with PAHs. In 1994-1995, EPA capped more than 50 acres of the harbor, placing a 3-foot thick layer of clean dredged sand on top of the contaminated sediments. Creosote seeps still occur on the beaches. To ensure the capped areas of the site continue to protect human health and the environment, EPA monitors contaminant concentrations in sediments, pore-water, and clam tissue collected from the beaches. Eagle Harbor is within the Suquamish tribe fishing area. The Suquamish tribe retains the right to harvest fish and marine invertebrates and have their fishery resource habitat areas protected. Region 10 Laboratory provided analytical support for treatment plant and monitoring events for more than 20 years. In 2014 about 20 clam composite samples were collected as part of a monitoring event. Region 10 developed a specialized method for preparing clam tissues for analysis called QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe). Lower sensitivity was obtained using GC/MS/secondary ion mass spectrometry (SIMS). Results were used to determine if shellfish are suitable for harvest. Response to Chemical Spill A chemical spill occurred on an Ohio River tributary, which lead to a Superfund emergency response. The On-Scene Coordinator (OSC) in charge of operations requested Region 5 Laboratory assistance with analysis of the chemical in the water. The chemical, (Tri-n-butyl)-n-tetradecylphosphoniumchloride, abbreviated TTPC, was detectable by liquid chromatography/mass spectrometry (LC/MS). A method was quickly developed and put to use within a month of the spill. Over 100 water samples have been analyzed to date to aid in the response effort. Over l2)nl if R analyses conducted by egional Laboratories for Emergency Response activities Spill Response in West Virginia In January 2014, the chemical 4-methylcyclohexanemethanol (MCHM) spilled in the Elk River in Charleston, West Virginia. The spilled chemical, used in coal preparation and processing, caused a drinking water advisory affecting approximately 300,000 people in nine counties. Other chemicals, such as polyglycol ethers (PPH), were later identified as part of the release. Region 3 Laboratory identified analytical techniques for MCHM and PPH in water, striving to meet health advisory levels to ensure the reliability of analytical methods used by public utilities and other organizations. Both GC/MS and high performance liquid chromatography/ultraviolet spectroscopy (HPLC/UV) were used along with other techniques to fully characterize the spilled material. Quick Analysis and Delivery of Results for Ringwood Mines Superfund Site Removal Response Region 2 Laboratory provided analytical support to the Ringwood Mines Superfund Site. The site was re- opened due to additional contamination found in residential areas originally deemed safe. The laboratory analyzed over 600 soil samples over a 6-month period, primarily for lead, in support of removal program activities at key residences. The laboratory provided validated results within 20 to 36 hours of each sample delivery. EPA 910-R-15-002 ------- FY2014 Annual Report Making a Visible Difference in Communities across the Country community-based activities to provide seamless assistance to communities, both urban and rural, while maximizing efficiency and results. Expand support of community efforts to build healthy, sustainable, green neighborhoods and reduce and prevent harmful exposures and health risks to children and underserved, overburdened communities. Environmental and public health impacts affect people most significantly where they live - at the community level. EPA is focused on providing better support to communities, especially in environmentally-overburdened, underserved, and economically-distressed areas where the needs are greatest. Regional Laboratories coordinate technical assistance and other resources across EPA Programs; with states, tribes, and local governments; and with other federal agencies to support communities as they pursue environmental improvements that enhance economic opportunity and quality of life. Air Sampling and Analysis from Southeast Chicago In response to air quality concerns in the Upper Midwest, Region 5 Laboratory actively monitored outdoor air pollutants and toxins. In investigating petroleum coke (petcoke) piles, the source of coal-like dust particles in the air, Region 5 scientists analyzed filters and collected soil and wipe samples from residences in southeast Chicago to determine whether air quality was impacting the health of homes. Petcoke piles have been a major concern in both Detroit and Chicago due to their recent storage in both cities. Samples were taken directly from the petcoke piles, analyzed, and compared with those taken from residential locations. The comparisons indicated a direct correlation to materials stored on-site at the refineries suggesting the piles were the source of contamination found in the residences. The findings resulted in capping and eventual removal of petcoke material from outside storage areas. This victory for human health is expected to protect many individuals from the impacts of poor air quality. Dye Study near Bridgeport, Connecticut Region 1 Laboratory scientists conducted two dye dilution studies on the Lower Housatonic River in Connecticut over a 2-week period in May 2014. EPA assisted in designing and conducting a dye study for the Stratford Water Pollution Control Facility and a second study at the Milford Housatonic Wastewater Treatment Plant, with discharges into the Housatonic River. Data have been used to help Connecticut and the U.S. Food and Drug Administration (FDA) to determine the extent to which treatment plants impact the Housatonic River and areas in the Long Island Sound. The study has also helped evaluate existing shellfish closure areas and determine whether they need to be modified. The Region 1 team designed the monitoring portion of the study and conducted nearfield tracking of dye concentrations around the outfalls. EPA staff also handled boat operations and tracked real-time concentrations of dye at fixed stations in transects throughout the estuary and into Long Island Sound. These studies involved long days of tracking dye on EPA boats to coincide with tides requiring staff to be on location in Connecticut for 2 weeks. The complexity of this study was due to the logistics of injecting dye at two plants, the vast extent of the study area, rough seas at times, and substantial media presence. EPA 910-R-15-002 ------- FY2014 Annual Report Making a Visible Difference in Communities across the Country Achieving Lower Detection Limits of Pesticides in Impaired Waters near City of Birmingham The city of Birmingham, Alabama and the Alabama Department of Environmental Management (ADEM) sought to remove Village Creek from the State's list of impaired waters. In accordance with Section 303(d) of the Clean Water Act, two sections of Village Creek have previously been identified as impaired due to the presence of the legacy pesticide dieldrin, which has been banned since 1987. Since the existing detection limit was higher than the human health criteria for dieldrin, Region 4 Laboratory improved the method by using a smaller extraction volume yielding a 100- fold decrease in detection limit. Hence, future sample results met the lower human health criteria and will determine potential delisting of the two sections of Village Creek. Over samples analyzed in FY2014 supporting EPA's Brownfields Program, which is designed to empower states, communities, and other stakeholders to redevelop abandoned industrial and commercial properties with potential contamination. Lead in Schools Initiative Several Regional Laboratories assisted Region 2 with the analysis of water samples from multiple schools and daycare centers for the Lead-in-Schools Initiative. Inductively-coupled plasma/mass spectrometry (ICP/ MS) was used for sample analysis. Regions participating included Region 2, Region 5, Region 6, Region 7, Region 9, and Region 10. EPA 910-R-15-002 ------- FY2014 Annual Report Launching a New Era of State, Tribal, and Local Partnerships Strengthen partnerships with states, tribes, local governments, and global communities central to the success of the national environmental protection program through consultation, collaboration, and shared accountability. By providing support at the regional level, opportunities abound to work in concert with states, tribes, and local entities in providing technical support. Types of activities where Regional Laboratories become involved include: Analytical support to states or tribes Assisting communities and volunteer monitoring groups with implementation of Citizen Science Providing training and technical support, including training in preparation of QAPPs. River and Stream Assessment for Wisconsin Region 5 provided assistance to the State of Wisconsin during the statewide river and stream sediment assessment. Region 5 Laboratory analyzed over 100 sediment samples for 10 different chemical tests, including both inorganic and organic analyses over a 3-year period. This effort was initiated to complete an assessment of the health of rivers and streams throughout the state. Training for New England Tribes Region 1 Laboratory organized and hosted a 2-day statistics training course in 2014 for about 20 New England environmental tribal staff. The course focused on the use of statistics for analyzing water quality data, with a focus on using "R," an open-source statistical program. Citizen Science becomes Reality One of Region 2 program goals is to raise the visibility level of citizen science in the region, support the formation of citizen science groups, and provide technical guidance and assistance to improve data quality of monitoring efforts. The citizen science pilot project in the New York-New Jersey Harbor serves as a blueprint for future citizen science efforts, which connected EPA scientists with citizens trained to collect and analyze water quality data, increasing knowledge about pathogen contamination of the harbor. Region 2 helped develop a "tool kit" for water quality monitoring. The components of the "tool kit" include: 1) QAPP template for planning monitoring projects 2) Field and laboratory datasheets to record observations/results and method information 3) SOPs for common water quality measurements 4) YouTube videos on sample collection procedures 5) Equipment loan program to provide critical monitoring equipment for collecting environmental data. Application and use of the region's citizen science "tool kit" was successfully tested during the pilot by four citizen science groups. The tools will be refined and made available on a formal equipment loan program in FY2015 for future citizen science groups, with an emphasis on Environmental Justice areas. EPA 910-R-15-002 ------- FY2014 Annual Report Embracing EPA as a High-Performing Organization Maintain and attract EPA's diverse and engaged workforce of the future with a more collaborative work environment. Modernize our business practices, taking advantage of new tools and technologies, and improve the way we work as a high-performing Agency. One of the most important regional and state laboratory partnerships is sharing unique expertise, when needed. In 2014, Regional Laboratories supported various projects. Laboratories relied on the expertise of other Regional Laboratories with unique capability/capacity. Collaboration with the Office of Research and Development (ORD) provided opportunities for scientists to expand their knowledge and skills through the Regional Research and Partnership Program. Increased Collaboration Tools Region 1 Laboratory developed a Regional Monitoring Network (RMN) to determine the condition of, and changes in, high-quality reference streams in New York and New England to climate change and other stressors on water temperature, flow, and macroinvertebrate communities. RMN is being duplicated at other EPA regions in the northeast, in close collaboration with other agencies and groups collecting similar data. Centralized data repositories and protocols are in development, through efforts of the U.S. Geological Survey (USGS), EPA, and others (for example: htto://wim. usqs. gov/NorEaSTA. Cost Savings for Air Sampling Using sorbent tubes, Region 7 scientists saved tremendous time and expense. Historically, air samples were collected in Summa canisters and followed a procedure delineated in the Compendium of Methods for the Determination of Toxic Organic Compounds in Ambient Air, Method TO-15. The Summa canisters were, and are currently, in limited supply due to high costs, costing thousands of dollars per canister. To purchase the canisters and manage rotating inventory advance planning is required. Region 7 developed a three-phased sorbent tube that realizes significant savings since the cost of tubes are significantly lower than canisters and tubes can be reused 100 times. Shipping costs for the tubes are also much lower. Laboratory Technical Information Group (LTIG): Enhancing Technical Knowledge of Laboratory Scientists In May 2014, LTIG held their 16th Annual Conference for laboratory scientists. LTIG, organized in 1998, invites laboratory scientists from all EPA Laboratories, communicating regularly through monthly conference calls and conducting annual conferences. LTIG's goal is to create a forum for technical discussion where chemists from all 10 Regional Laboratories and other EPA offices can communicate and exchange ideas on analytical procedures and problems. 'J Regional Scientists worked with ORD under the Regional Research Partnership Program (R2P2) - a program to enhance collaboration and share knowledge between ORD and the Regions EPA 910-R-15-002 ------- FY2014 Annual Report Embracing EPA as a High-Performing Organization Reduce Solvent Use by Testing New Technology Region 7 chemists collaborated with multiple partners to provide analytical data on two different solid-phase extraction (SPE) products in response to recent Clean Water Methods update. Chemists have been working a new sorbent-coated stir bar technology (Twister) in the analysis of urban stream water monitoring for four years. A method for semi- volatile analytes, which included personal care products and pesticides, was developed and results presented. Chemists also worked with the Office of Water providing comments on method revisions for EPA Methods 608, 624, and 625 resulting in the final publication, which closed May 2015. Region 7 chemists began working with the Independent Laboratories Institute (III) of the American Council of Independent Laboratories (ACIL) and meeting with Horizon Technologies to test the SPE technology with Horizon equipment. Ill was tasked with coordinating large projects to benefit all laboratories and vendors. The first major project, a multi-laboratory study, was determined the feasibility of using SPE techniques using EPA water methods with the Office of Water as the ultimate customer, designed to produce the data quality required by alternate test procedures. Vendors had to identify at least three laboratories, including one of their own, to participate in this study, which included 25 laboratories from industry, government, and academia. Region 7 chemists participated in two phases of the project, using stir bar technology and Horizon SPE products. Electronic results were provided to an anonymous sample vendor who consolidated the data and performed statistical analysis. The results of the study proved that the SPE technique was a viable candidate to replace the traditional separatory funnel extraction. The ultimate benefit to the Agency is the flexibility to use newer technology that significantly reduces the use of hazardous solvents and the resulting hazardous waste. Once the method revisions are approved, new extraction methods will be allowed for enforcement of the CWA with an eye on green chemistry. Method Enhancement or Development Projects completed in FY2014 EPA 910-R-15-002 ------- FY2014 Annual Report Embracing EPA as a High-Performing Organizatio Regional Laboratories Support ORD Hydraulic Fracturing Study Regional Laboratories coordinated with EPA's ORD on a 2-year research project directed by U.S. Congress to study the relationship between hydraulic fracturing and drinking water resources. Natural gas plays a key role in the future of our nation's clean energy. Recent advances in drilling technologiesincluding horizontal drilling and hydraulic fracturinghave made vast reserves of natural gas economically recoverable in the U.S. Responsible development of America's oil and gas resources offers important economic, energy, security and environmental benefits. Hydraulic fracturing is a well-stimulation technique used to maximize production of oil and natural gas in unconventional reservoirs, such as shale, coal beds, and tight sands. During hydraulic fracturing, specially-engineered fluids containing chemical additives are pumped under high pressure into the well to create and hold open fractures in the formation. These fractures increase the exposed surface area of the rock in the formation and stimulate the flow of natural gas or oil to the wellbore. The use of hydraulic fracturing concerns have increased due to potential environmental and potential risks to drinking water. Region 3 and Region 7 Laboratories provided chemical analysis and field support forsemi- volatiles, diesel-range organics, gasoline-range organics, glycols and other unknown identifications. Mercury at Trask Forest Land use is a critical parameter controlling environmental mercury (Hg) cycling. Factors that influence the mobility (for example, water flow, sediment mobilization), bioavailability of inorganic mercury (particulate-versus-filtered fractions), and other variables associated with methyl mercury production (for example, sulfate, organic carbon) are distinctly associated with numerous land-use practices that may allow management actions to influence bioaccumulation in aquatic habitats. Methylmercury (MeHg), which is produced by anoxic bacteria, primarily sulfate-reducing bacteria (SRB), is the more toxic and bioaccumulative form of Hg. Timber harvesting is a widespread land-use activity in the Pacific Northwest (PNW). There are approximately 60,000 square miles of harvestable forestland in PNW, which accounts for nearly 25 percent of the region's total land area. Thus, Hg distribution, cycling, and bioaccumulation in PNW may be strongly influenced by the magnitude and scale of regional timber activities. Region 10 has been collaborating with USGS since 2013 to study the effects of logging practices on mercury processing and transport in Oregon's Trask River watershed. Region 10 Laboratory supported the research with MeHg and ultra-low-level Hg analyses (519 analyses total) of surface waters collected to study the effects of different harvesting techniques, such as clearcutting and selective harvesting. The analyses were challenging due to complexities of the MeHg method and the need to maintain a pristine laboratory environment to accurately measure to very low levels in samples. One preliminary conclusion is that dissolved mercury transports out of watersheds that were clear- cut is significantly greater than watersheds that have not been harvested. The remaining analysis is to evaluate changes in mercury loading from the watersheds. Once this work is complete, the study will be published in a peer-reviewed journal by USGS and EPA. EPA 910-R-15-002 ------- FY2014 Annual Report Embracing EPA as a High-Performing Organization Preserving Land at Longhorn Army Ammunitions Site To assist with the analysis of related samples at the Longhorn Army Ammunitions site, Region 6 Laboratory developed a method for analyzing all six dinitrotoluene (DNT) isomers. These chemicals were suspected to be breakdown products from munitions stored on base. An analytical procedure using a gas chromatograph-triple quadrupole mass spectrometer (GC/MS/MS) was developed to successfully validate extractions down to 20 parts per trillion. Development of Dissolved Gases or Light Hydrocarbon Method Region 6 coordinated a three-laboratory round robin method and analysis study for light hydrocarbons (LHCs) in water by gas chromatography/flame ionization detector (GC/FID). Region 6 Laboratory, an instrument vendor, and a state laboratory exchanged samples and coordinated instrument conditions over a 1-week period. The results were statistically analyzed for precision and accuracy parameters. Currently, no official EPA method for this analysis; however, Regions 5, 6, and the Office of Solid Waste and Emergency Response (OSWER)/Office of Resource Conservation and Recovery (ORCR) have a pending proposal to further develop this effort into an official EPA method. New Method to Measure Lower Levels of Lead in Soils The Centers for Disease Control (CDC) observed continued health effects in people due to lead in their blood even though presence of lead at historical remediation sites were well below the action levels needed to protect human health. Socioeconomically disadvantaged children are particularly susceptible to health effects due to the persistence of lead bound to small particulate matter in their environment and the tendency for children to put "dirty" hands to their mouth. Therefore, EPA is reevaluating the action level for lead in soils. In anticipation of the need to detect lead in soils at lower concentrations, Region 4 Laboratory is developing a new method for preparing soil samples. The new method focuses on soils most likely to affect at-risk human subjects. This method comprises sieving the raw sample to a particular particle size and extracting the lead using the human amino acid, glycine, to mimic effects of digestion. Historical studies will be repeated with the new methodology to evaluate site compliance with the new action level for lead. Field Analyses conducted in 2014 Almost double from 2013 EPA 910-R-15-002 ------- FY2014 Annual Report Working toward a Sustainable Future Vance sustainable environmental outcomes and optimize economic and social outcomes through Agency decisions and actions, which include expanding conversation on environmentalism and engaging a broad range of stakeholders. Regional Laboratories continue to explore opportunities to reduce chemical usage or improve processes. Reducing Solvents for EPA Methods 608 and 625 Region 7 chemists developed several solid-phase extraction methods that reduce solvent usage while maintaining or improving detection limits. In FY2014, extensive method development was culminated as a partnership with two different vendors and the ACIL. Region 7 was one of 25 laboratories participating in a two-phase study, which provided technical response to the comment period for the Code of Federal Regulations (CFR) update of EPA Methods 608 and 625. Supporting Enforcement Actions related to Ozone- Depleting Compounds Many people often take for granted the chemical safety of their home appliances. Particularly, chemicals that may come in contact with food or the air we breathe. This year, the Criminal Investigation Division (CID) requested Region 6 Laboratory to assist in an air refrigerant purity investigation, by analyzing samples of pressure cylinders or containers used to hold gases at a pressure greater than that of the atmosphere, containing an air conditioning refrigerant. R-22, a non-flammable, ozone-depleting compound, was listed as the main refrigerant; however, other chemicals are suspected to be present in these cylinders. R-22 is currently being phased out in products used for domestic consumption under the Montreal Protocol Provisions of the Clean Air Act (CAA). The limited remaining amounts of R-22 have resulted in replacement refrigerants being sold at lower costs. However, these substitutes may be mixtures containing other flammable and dangerous compounds, such as propane and butane mixtures that can damage the refrigerator's air conditioning system. Explosions and fires have been reported when such alternative refrigerants are used, making them extremely dangerous to both animals and humans. Region 6 Laboratory developed a new technique for transferring the cylinder contents to the instrument. As suspected, none of the samples analyzed contained R-22 above the reporting limit. However, propane, a hydrocarbon and flammable refrigerant was discovered. Hydrocarbon refrigerants pose a particular hazard, especially when they are stored in containers that are not properly labeled. Analytical support provided by Region 6 Laboratory helped ensure the container contents are used with the necessary precautions; thus, reducing the potential of injury to those that use the refrigerant cylinders. EPA 910-R-15-002 ------- ------- FY2014 Annual Report Section III FY2014 Laboratory Accomplishment Results Summary &EPA United States Environmental Protection Agency EPA 910-R-15-002 ------- FY2014 Annual Report FY2014 Laboratory Accomplishment Results Summary This section summarizes a number of the common support services provided by the RLN. Because of the unique nature of the support provided by Regional Laboratories, the ideal Regional Laboratory scientist is one part research scientist and one part production scientist. Regional Laboratory scientists are capable of developing methods (often with short lead times), focusing on quality control, and operating under demanding delivery schedules. Regional Laboratory staff support diverse and challenging requests. During FY2014, RLN supported more than 143,000 analyses. The distribution of work by the RLN is shown in Figures 1 and 2. These totals exclude Quality Control (QC) samples, which add an additional 20%. Figure 1. Analytical Support to EPA Programs in FY2014 (143,234 Total Analyses) Emergency Response 5.4%. Figure 2. FY2014 U.S. EPA Regional Laboratories Analyses Provided by Program (143,234 Total Analyses) 90000 82428 7764 7722 1638 1613 706 71 52 In keeping with prior years, Superfund program continues to be the largest volume requestor of analytical services (57.5%), followed by Water Programs (28.2%). Emergency Response program support continues to be significant at 5.4%, with RLN laboratories analyzing 7,722 samples in conjunction with time- critical responses to environmental disasters, hazardous materials releases, priority contaminant removals, and other threats to human health and/or the environment, which aided in timely and cost-effective decision-making in the field. All 10 Regional Laboratories augmented the National Enforcement Investigations Center's (NEIC's) capacity in support of important criminal cases, analyzing 424 criminal samples during the year. EPA 910-R-15-002 ------- FY2014 Annual Report FY2014 Laboratory Accomplishment Results Summary Figure 3 summarizes the number of analytical projects supported by RLN according to EPA program element. Collectively, RLN supported 1,474 projects. Figures. Projects/Sites Supported by Regional Laboratory Data FY2014 by Program Element (1 ,474 Total Sites/Projects Supported) Water- Program Implementation (REMAP, TMDLF TOXNET, etc.) ^^^^_ Water- Enforcement Water - Drinking Water Compliance and Emergencies J 1 160 J 115 92 Superfund -Removal ( 72 Other » fl 48 Superfund -Emergency Response j 37 Air- Program Implementation (monitoring, permits, etc.) .j) 32 Criminal Investigation _i 24 RCRA - Enforcement Lj 18 RCRA- Corrective Action J 15 LUST J 13 TSCA- Remedial J 11 Brownfields J 10 TSCA - Enforcement | 5 Pesticides | 4 Air -Enforcement 2 592 A significant amount of work supported during the year required methods be developed specifically to address the unique needs of a particular region (Figure 4). Often, methods developed by a region to address a local environmental challenge are mobilized in other regions as their benefit is realized and/or as the need arises. Figure 4. Method Development Project Support to EPA Programs in FY2014 (125 Methods) eslicide' 1.6% EPA 910-R-15-002 ------- ------- FY2014 Annual Report Section IV Regional Laboratories Capabilities Tables FY2014 &EPA United States Environmental Protection Agency ------- FY2014 Annual Report ll Regional Laboratories Core Capabilities FY2014 I. Chemistry Analyte/Group Name Sample Media Analytical Technique INORGANIC CHEMISTRY Acidity Alkalinity Asbestos Anions Chromium, Hexavalent (Cr+6) Cyanide, Amenable Cyanide, Total Fluoride Hardness Mercury, Total Mercury (TCLP) Metals, Total Metals (TCLP) Metals, Total Metals (TCLP) Metals, Total Metals (TCLP) Nitrogen (Ammonia) Water Water Solids/Bulk material Soil/Sediment Water Water Water Soil/Sediment Water Soil/Sediment Water Soil/Sediment Water Soil/Sediment Waste Water Water Water Water Water Water Water Soil/Sediment Soil/Sediment Tissue (fish &/or plant) Tissue (fish &/or plant) Waste (oil, drum, etc.) Waste (oil, drum, etc.) Soil/Waste (oil, drum, etc.) Soil/Waste (oil, drum, etc.) Water Soil/Sediment Tissue (fish &/or plant) Waste (oil, drum, etc.) Soil/Waste (oil, drum, etc.) Water Soil/Sediment Tissue (fish &/or plant) Waste (oil, drum, etc.) Soil/Waste (oil, drum, etc.) Water Soil/Sediment Tissue (fish &/or plant) Waste (oil, drum, etc.) Soil/Waste (oil, drum, etc.) Water Soil/Sediment Water Titrimetric Titrimetric PLM PLM 1C Titrimetric Colorimetric Colorimetric 1C 1C Colorimetric Colorimetric Colorimetric Colorimetric Colorimetric ISE 1C Colorimetric Titrimetric ICP/Calculation CVAA Direct Hg Analysis CVAA Direct Hg Analysis CVAA Direct Hg Analysis CVAA Direct Hg Analysis CVAA Direct Hg Analysis ICP/AES ICP/AES ICP/AES ICP/AES ICP/AES GFAA GFAA GFAA GFAA GFAA ICP/MS ICP/MS ICP/MS ICP/MS ICP/MS Colorimetric Colorimetric Electrode Regional Capability 1 X X X X X X X X X X X X X X X X X X X X X X X X X X X X 2 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 3 X X X X X X X X X X X X X X X X X X X X X X X X X X X X 4 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 5 X X X X X X X X X X X X X X X X X X X X X X X X X X 6 X X X X X X X X X X X X X X X X X X X X X X X X X X X 7 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 8 X X X X X X X X X X X X X X X X X X X X X X X X X X X X 9 X X X X X X X X X X X X X X X X X X X X X X X 10 X X X X X X X X X X X X X X X X X X X X X X X X X X X X ------- FY2014 Annual Report Regional Laboratories Core Capabilities FY2014 I. Chemistry Analyte/Group Name Sample Media Analytical Technique INORGANIC CHEMISTRY Nitrogen (NO3 &/or NO2) Nitrogen, Total Kjeldahl Perchlorate Phosphorus, Ortho Phosphorus, Total Sulfate Sulfide Water Soil Water Soil Water Soil Water Soil Water Water, Soil/Sediment Water Water Water Water Soil Water Soil Water Soil Water Soil Water Water Colorimetric Colorimetric 1C 1C Colorimetric Colorimetric 1C 1C 1C with LC/MS confirmation LC/MS LC/MS/MS Colorimetric 1C Colorimetric Colorimetric 1C 1C Turbidimetric Turbidimetric Colorimetric Colorimetric 1C, Turbidimetric Titrimetric Regional Capability 1 X X X X X X X X X X X 2 X X X X X X X X X X 3 X X X X X X X X X X X X 4 X X X X X X X X X X X X 5 X X X X X X X X X X X X X 6 X X X X X X X X 7 X X X X X X X X X X X X X 8 X X X X X X X X 9 X X X X X X X X X X X X 10 X X X X X X X X X X X X ORGANIC CHEMISTRY BNA BNA (TCLP) BNA (TPH) BOD COD EDB & DBCP Herbicides Herbicides (TCLP) Oil & Grease Pesticides/PCBs Pesticides (TCLP) Phenolics Water Soil/Sediment Waste (oil, drum, etc.) Tissue (fish &/or plant) Solid/Waste Water Soil/Sediment Water Water Water Water Water Soil/Sediment Waste (oil, drum, etc.) Tissue (fish &/or plant) Solid/Waste Solid/Waste Water Soil/Sediment Water Soil/Sediment Waste (oil, drum, etc.) Tissue (fish &/or plant) Solid/Waste Water Soil/Sediment GC/MS GC/MS GC/MS GC/MS GC/MS GC/MS or GC GC/MS or GC Membrane Electrode Photometric Colorimetric GC/ECD GC/ECD; GC/NPD GC/ECD; GC/NPD GC/ECD; GC/NPD GC/ECD; GC/NPD GC/ECD HPLC/UV Detection Gravimetric Gravimetric GC/ECD GC/ECD GC/ECD GC/ECD GC/ECD Colorimetric Colorimetric X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X EPA 910-R-15-002 ------- FY2014 Annual Report ll Regional Laboratories Core Capabilities FY2014 I. Chemistry Analyte/Group Name Sample Media Analytical Technique ORGANIC CHEMISTRY PAHs TOO TOO VOA VOA VOA (TCLP) VOA (TPH) Water Soil/Sediment Air Tissue (fish &/or plant) Waste (oil, drum, etc.) Water Soil Water Water Soil/Sediment Air Waste (oil, drum, etc.) Water Soil/Sediment Waste (oil, drum, etc.) Solid/Waste Water Soil/Sediment GC/MS GC/MS GC/MS GC/MS GC/MS Combustion/IR Combustion/IR UV/Persulfate GC/MS GC/MS GC/MS GC/MS GC GC GC GC/MS GC/MS or GC GC/MS or GC Regional Capability 1 X X X X X X X X X X 2 X X X X X X X X X 3 X X X X X X X X X X 4 X X X X X X X X X X X X X X X X 5 X X X X X X X X X X X 6 X X X X X X X X X X X 7 X X X X X X X X X X X X X X 8 X X X X X X X X X X X X X X X X 9 X X X X X X X X X 10 X X X X X X X X X X X X II. Physical and Other Determinations Flash Point Conductivity Ignitability pH Solids, Non-Filterable Solids, Percent Solids, Total Solids, Total Dissolved Solids, Total Volatile Turbidity Aqueous/Liquid Waste (oil, drum, etc.) Water Soil/Sediment Waste (oil, drum, etc.) Water Soil/Sediment Waste (oil, drum, etc.) Water Soil/Sediment Water Water Water Water Pensky-Martens or Seta Specific Conductance Ignitability of Solids Pensky-Martens or Seta Closed Cup Electrometric Electrometric Electrometric Gravimetric Gravimetric Gravimetric Gravimetric Gravimetric Nephelometric X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X III. Biology/Microbiology Coliform, Total Coliform, Fecal E. coli Toxicity (Acute & Chronic) Heterotrophic PC Water, Soil &/or Sludge Water, Soil &/or Sludge Water, Soil &/or Sludge Water Water Various Various Various Fathead, Ceriodaphnia Various X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X ------- FY2014 Annual Report Regional Laboratories Unique Capabilities FY2014 REGION 1 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Inorganic Anions Mercury Metals Perchlorate Water Water, Tissue Water, Sediment, Soil, Waste (drum), Paint, Dust, Cosmetics Water 1C (EPA Method 300.0) Direct Mercury Analyzer (Thermal Decomposition, Amalgamation & Atomic Absorption Spectrophotometry) EPA Method 7473 XRF (EPA Method 6200) LC/MS/MS (EPA Method 331.0) Water Superfund, Water Superfund, TSCA (Pb) Superfund/Water Field Screening and Laboratory Testing ORGANIC CHEMISTRY Carbonyls 1 ,4-Dioxane Ethylene Glycol Explosives Oil Identification Organic Compounds Oxygenated Compounds/Benzene PAHs PCBs Pentachlorophenol Pesticides/PCBs Pharmaceuticals and Personal Care Products (PPCP) VOCs Air Water Water Water, Soil Water Solid, Liquid Fuel Soil/Sediment Air, Wipes Soil, Sediment Water, Soil, Sediment, Waste (drum) Water, Soil, Sediment, Waste (drum) Water Air (mini-cans) Water, Soil, Air HPLC (EPA Method TO-11A) GC/MS Purge & Trap (EPA Method 8260) GC HPLC (EPA Method 8330) GC/FID(ASTMD-3415-79) FTIR IR (RFG Inspector's Manual) Immunoassay (EPA Method 4035) GC/ECD (EPA Method 3508A) Immunoassay (EPA Method 4010) GC/ECD (EPA Method 8081 A/8082) GC/ECD (EPA Method 680) LC/MS/MS GC/MS (EPA Method TO-15) GC/ECD/PID Air Superfund Superfund Superfund Superfund - ERB Air Superfund Air/Superfund Superfund Superfund Superfund Water Superfund Superfund Unknown ID PHYSICAL AND OTHER DETERMINATIONS Grain Size Loss on Ignition (LOI) Percent Lipids Soil, Sediment Sediment Tissue Sieve (Modified ASTM) Gravimetric Superfund, Water Water Region 1 SOP BIOLOGY/MICROBIOLOGY Enterococci Chlorophyll a Toxicity (Acute) Ambient water Ambient water Sediment Enterolert/EPA Method 1600 EPA 445.0 C. dilutus, H. azteca Ambient monitoring Ambient monitoring Water, Superfund Bulk sediment ------- FY2014 Annual Report ll Regional Laboratories Unique Capabilities FY2014 REGION 2 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY CO NOx SO2 Percent Sulfur Vanadium Air/N2 Air/N2 Air/N2 Fuel Oil Fuel Oil EPA Reference or Equivalent Method as in 40 CFR Part 58 EPA Reference or Equivalent Method as in 40 CFR Part 58 EPA Reference or Equivalent Method as in 40 CFR Part 58 ASTM D4294 ICP/AES Air Air Air Air Air Dry ashing at 525°C ORGANIC CHEMISTRY Asphaltenes (Hexane Insolubles) Methane, Ethane, Ethene Ozone Precursors (hydrocarbons) Pesticides Total Petroleum Hydrocarbons Fuel Oil Water Air Wipes Water, Solid ASTM 3279 GC/FID GC/MS/FID LC/MS/MS and GC/MS Hexane Extraction (EPA Method 1664) Air SF/RCRA Air General Water PHYSICAL AND OTHER DETERMINATIONS Density Grain Size Particulates (Fine) Percent Volatile Matter Percent Water Viscosity Ink, Paint Solid Solid Air Ink, Paint Fuel Oil ASTM D1 475 Pipet Method Hydrometer Method (based on ASTM D422-63) EPA Reference or Equivalent Method as in 40 CFR Part 58 ASTM D2369 ASTMD4017 ASTM D88 Air Superfund, Water Superfund, Water Air Air Air Air BIOLOGY/MICROBIOLOGY Cryptosporidium DMA - qPCR (Enterococcus) DNA-qPCR E. coli DNA, Markers, Various Enterococcus Group Giardia mColiblue24 Enterolert w/Quantitray Colilert 18/Colilert w/Quantitray Water Water (Fresh & Marine) Water (Fresh & Marine) Water (Fresh & Marine) Water Water Water Water Water Fluorescent Microscopy (EPA Method 1623) EPA/Cepheid Methodology EPA/CDC Protocols Geese, Gull, Cow, HF183, Gen Bacteroidales Membrane Filtration Fluorescent Microscopy (EPA Method 1623) MF/Hach Defined Substrate Technology Defined Substrate Technology Water Water Water Water Water Water Water Water Water ------- FY2014 Annual Report Regional Laboratories Unique Capabilities FY2014 REGION 3 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments ORGANIC CHEMISTRY Nitroaromatics & Nitroamines Nitroglycerine Nitrogen, Total Chemical Warfare Agents PCB Congeners Water, Soil/Sediment Water, Soil/Sediment Water Water/Solid/Wipe Water, Soil/Sediment, semi-permeable membrane device (SPMD) HPLC HPLC Colorimetric GC/MS HR GC/MS Water Water Emergency Response Method 8330 Method 8332 Method 1668C PHYSICAL AND OTHER DETERMINATIONS ID Ozone-Depleting Compounds ID Unknowns Alcohols ID Unknowns Propellants/Aerosols Bulk Mercury Water Soil/Sediment Water, Soil/Sediment Wastes FTIR Density FTIR FTIR FTIR FTIR Air Enforcement Superfund, RCRA Water RCRA Screening it, identify unknowns Screening it, identify unknowns When necessary for ignitability Screening it, identify unknowns BIOLOGY/MICROBIOLOGY Benthic Macroinvertebrate Marine/Estuarine Benthic Invertebrate Taxonomy Freshwater Invertebrate Specimens or Unsorted Sediment Identification EPA EMAP Protocols Water Organisms identified to species or lowest taxonomy possible REGION 4 INORGANIC CHEMISTRY Chromium (+6) Mercury, Total - Ultra-Low Detection Level Metals, Total Metals (TCLP) Soil/Sediment Water Tissue Soil/Sediment Waste (oil, drum, etc.) Air Soil/Waste (oil, drum) Std Method 3500 CrD CVAF CVAF CVAF ICP/MS Hi-Vol Filters ICP/MS DW, Superfund Water Water, Superfund Water, Superfund RCRA Air RCRA Method 1631 Appendix 1631 Appendix 1631 not commonly available not commonly available not commonly available ORGANIC CHEMISTRY Freon Products Natural Attenuation Analytes PCB Congeners Toxaphene Congeners Ultimate BOD Canister & Air Water Water Soil/Sediment Tissue Water/Soil Water GC/MS GC/FID None None None GC/NIMS (EPA Method 8276) Membrane Electrode (Std Method 521 OC) Air, OECA Superfund Superfund, RCRA Superfund, RCRA Superfund, RCRA Water, Superfund Water Special analysis technique developed for criminal investigations of illegal Freon Methane, ethane, ethene High resolution GC/MS High resolution GC/MS High resolution GC/MS 6 parlars, 2 breakdown products BIOLOGY/MICROBIOLOGY Chlorophyll Water Water ------- FY2014 Annual Report ll Regional Laboratories Unique Capabilities FY2014 REGION 5 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Bromide/Chloride Ratio Chloride Metals Brine Samples Soil/Sediment Suspended Particulate Matter 1C & related characterization techniques; ion balance 1C ICP/MS Water, UIC, &SDWA Sediment Air Difficult analyses Analysis of TSP, Pm10,PM2.5 filters for metals ORGANIC CHEMISTRY Nonylphenol (NP), NP-1 and 2- ethoxylate, octyphenol, & bisphenol-A Nonylphenol (AP), AP-1 and 2- ethoxylate, octyphenol, & bisphenol-A Nonylphenol (NP), NP-1 and 2- ethoxylate, octyphenol Bisphenol-A Nonylphenol carboxylates Long chain NP, NPEOs (n=3- 18) COD PCBs PCB Congeners Purgeable 1,4-Dioxane & Tetrahydrofuran (THF) Various analytes (VOAs, SVOCs, & Pesticides/PCBs) 129 Toxic Industrial Chemicals (TICs) & CWAdegradants (107 validated) Aldicarb, aldicarb sulfone, aldicarb sulfoxide, carbofuran, oxamyl, methomyl, &thiofanox Aldicarb, bromadiolone, carbofuran, oxamyl, & methomyl Thiodiglycol Thiodiglycol Thiodiglycol Diethanolamine, triethanolamine, n-methyldiethanolamine, & methyldiethanolamine Water Soil/Sediment Water Water Water Water Soil/Sediment Water, Oil, Soil, Wipes Water. Sludge Water Water, Soil/Sediment Drinking Water Water Water Water Soil Wipes Water GC/MS(ASTMD7065-11) GC/MS (8270 modified/Internal SOP) LC/MS/MS (ASTM D7485-09) LC/MS/MS (ASTM D7574-09) LC/MS/MS LC/MS/MS (ASTM D7742-1 1) Colorimetric 8082 (GC/EC) GC/MS/MS, GC/NCI/MS Method 624-Dioxane (Wide- Bore Capillary Column GC/ MS) ESAT FASP Methods GC/EC for VOAs, SVOCs, & Pesticides/PCBs (XRF for metals) LC/MS/MS Library Screening LC/MS/MS, ASTM 7645-10 LC/MS/MS, ASTM 7600-09 LC/MS/MS, CRL SOP MS015 LC/MS/MS, ASTM E2787-1 1 LC/MS/MS, ASTM E2838-1 1 LC/MS/MS, ASTM D7599-09 Water Water Water Water Water Water Sediment TSCA RCRA, SF, TSCA, Water Superfund Superfund WSD, NHSRC NHSRC NHSRC NHSRC NHSRC NHSRC NHSRC Endocrine disrupter - high concentration method (ppb) Endocrine disrupter Endocrine disrupter low-level method (ppt) Endocrine disrupter low-level method (ppt) Endocrine disrupter Endocrine disrupter Aroclor-specific TSCA reg. Compliance method & multiple action levels Compare with HRGC/HRMS method Specific analyte analysis method Fast TAT onsite; Screening or better data; Fast extraction for organics Library search routine developed under CRADA with Waters Corp. Now use NIST LC/MS/MS Library of over 2,000 analytes SAP Method SAP Method SAP Method SAP Method SAP Method SAP Method ------- FY2014 Annual Report Regional Laboratories Unique Capabilities FY2014 REGION 5 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments ORGANIC CHEMISTRY Dioctyl Sulfosuccinat (DOSS) in sea water Dipropylene glycol monobutyl ether ðylene glycol monobutyl ether in seawater Bromodiolone, brodifacoum, diphacinone, & warfarin in water Diisopropyl methylphosphonate, ethyl hydrogendimethylamidophosph ate, ethyl methylphosphonic acid, isopropyl methylphosphonic acid, methylphosphonic acid, and pinacoiyl methylphosphonic acid DIMP, EMPA, IMPA, MPA, PMPA Seawater Seawater Water Water Soil LC/MS/MS, ASTM D7730-1 1 LC/MS/MS, ASTM D7731-11 LC/MS/MS, ASTM D7644-1 1 LC/MS/MS, ASTM 7597-09 LC/MS/MS, ASTM WK34580 NHSRC/SF NHSRC/SF NHSRC NHSRC NHSRC SAP Method SAP Method SAP Method SAP Method SAP Method PHYSICAL AND OTHER DETERMINATIONS Corrosivity by pH Particle Size Water Content Paint Filter Test Specific Gravity Synthetic Precipitation Leaching Procedure (SPLP) Hazardous Waste Soil/Sediment Hazardous waste Paints and coatings Soil/Sediment Solid Waste SW8461110 Particle size analyzer provides continuum of sizes - CRL SOP SW846 Appendix IV of the Corps of Engineers Engineering Manual (F10-F22) SW8461312 RCRA GLNPO, Water-Sediment RCRA, Superfund RCRA, Superfund Sediment RCRA, Superfund Waste characterization For modelling and soil migration calcs Support for flashpoint For all TCLP analytes except herbicides ------- FY2014 Annual Report ll Regional Laboratories Unique Capabilities FY2014 REGION 6 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Ammonia Ozone NOx SOx Trace level Hex Chrome Perchlorate Metals by X-Ray Fluorescence Air (passive coated filter) Air (passive coated filter) Air (passive coated filter) Air (passive coated filter) Water Water Soil 1C 1C 1C 1C IC/UV IC/MS/MS portable XRF CAA CAA CAA CAA Water Water Superfund, RCRA Ogawa passive air collection device Ogawa passive air collection device Ogawa passive air collection device Ogawa passive air collection device field screening ORGANIC CHEMISTRY Fingerprint (pattern recognition) Incidental PCBs Chemical Warfare Agents PAMS (C2s and C3s identified) PCBs (Aroclor) PAHs (trace) Chemical Warfare Agents - degradation products VOCs by OVM Organophosphorous Pesticides (OPPs) High-level waste Oil Fuel Water Soil/Sediment Waste Water/Solid/Wipe Air Electrical cable Water/Solid/Oil Water Air Water Soil/Sediment GC/MS GC/MS GC/MS GC/MS; Method 680 Homologue Series GC/MS; Method 680 Homologue Series GC/MS; Method 680 Homologue Series GC/MS GC/MS/FID (split) GC; Separation, extraction, analysis of individual components; Mod of program -specific technique GC/QQQ LC/MS/MS GC/MS GC/NPD GC/NPD GC/NPD RCRA RCRA RCRA TSCA, RCRA TSCA, RCRA TSCA, RCRA Emergency Response CAA TSCA RCRA, Superfund Emergency Response CAA CWA, RCRA, Superfund RCRA, Superfund RCRA, Superfund grouped by number of chlorine grouped by number of chlorine grouped by number of chlorine C2s and C3s are individually quantitated Toluene is extraction solvent passive air monitoring PHYSICAL AND OTHER DETERMINATIONS Corrosivity by pH Waste Method 1110 Corrosivity Toward Steel RCRA ------- FY2014 Annual Report Regional Laboratories Unique Capabilities FY2014 Analyte/Group Name INORGANIC CHEMISTRY CO NOx SO2 03 In-vitro Bioassessibility Assays for Arsenic & Lead in Soil ORGANIC CHEMISTRY Chlordane Herbicides Pesticides VOCs PCBs Pharmaceuticals and Personal Care Products (PPCPs) PAHs, Pesticides, Herbicides VOCs VOCs from In-situ Chemical Oxidation Sites BIOLOGY/MICROBIOLOGY E. coli Enterococci Heterotrophic Bacteria Chlorophyll a Invertebrate Taxonomy Marine/Estuarine Benthic Taxonomy Sample Media Air Air Air Air Soil Air(PUF) Water, Soil/Sediment Water, Soil/Sediment, Tissue Air Canister Air Sorbent Tube Water Soil/Sediment, Waste Water Water Water, Soil, Air Water Water (drinking/waste/ ambient) Water Water Ambient water Invertebrates Benthic Organisms REGION 7 Analytical Technique 40 CFR Part 58 40 CFR Part 58 40 CFR Part 58 40 CFR Part 58 ICP/MS-ICP/AES GC/ECD (EPA Method TO- 4A) GC/ECD GC/ECD GC/MS (EPA Method TO-14 &TO-15) GC/MS (EPA Method TO-17) GC/MS GC/ECD LC/MS/MS Twister GC/MS Stir Bar Sorbtive Extraction (solventless extraction) GC/MS Mobile Laboratory GC/MS qPCR qPCR Plate Count - Standard Methods EPA 445.0 EPA EMAP Protocols Supported Program(s) Air Air Air Air Superfund/RCRA Special Project Water Water Air/Superfund Air/Superfund Superfund/ORD Superfund/ORD Water Water Superfund Superfund Water Water Water Ambient monitoring Water Water Comments OAQPS Protocol Gas Verification Program OAQPS Protocol Gas Verification Program OAQPS Protocol Gas Verification Program NIST Standard Reference Photometer SUPR Exposure/Toxicity Assessment Use Attainability Analysis (UAA) Use Attainability Analysis (UAA) Air Toxics Air Toxics In-situ Chemical Oxidation Site Support Rapid Site Screening Endocrine disrupters Use Attainability Analysis (UAA) Rapid Site Characterization Improved Precision of VOC Samples from In-situ Chemical Oxidation Sites 2008 NFWA Heterotrophic Bacteria Organisms identified to species or lowest taxonomy possible ------- FY2014 Annual Report ll Regional Laboratories Unique Capabilities FY2014 REGION 8 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Silica Gadilinium Water Water Colorimetric ICP/MS Water/Superfund Water/Superfund Wastewater Indicator ORGANIC CHEMISTRY Alcohols Chlorophyll Endothall TPH (VOA & BNA) LC/MS/MS Pesticides Low-level Pesticides/CLLE Metals - Arsenic/Selenium speciation Pharmaceuticals and Personal Care Products (PPCPs) Waste Indicator Compounds Total Petroleum Hydrocarbons- Diesel Range Organics VOAs Water Water Water Water, Soil/Sediment Water Water Water, Soil, Tissue Water Water Water, Soil Water, Soil/Sediment GC/FID HPLC GC/MS GC/MS or GC/FID LC/MS/MS GC/MS IC/ICP/MS LC/MS/MS GC/MS GC/FID GC/PID/ELCD Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Water/Superfund Monitoring for States & Tribes Monitoring for States & Tribes Speciation data needed for risk assessment Endocrine disrupters Monitoring for States & Tribes Hydro-fracking BIOLOGY/MICROBIOLOGY Bacteria (Arsenic-Reducing) Bacteria (Iron-Reducing) Bacteria (Sulfate-Reducing) Bacteria (Clostridium perfringens) Water, Sediment Water, Sediment Water, Sediment Water MPN MPN MPN Membrane Filtration Water/Superfund Water/Superfund Water/Superfund Water/Superfund ------- FY2014 Annual Report Regional Laboratories Unique Capabilities FY2014 REGION 9 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Ferrous Iron Mercury, Vapor, Particulate, & Reactive Methyl mercury Metals (with mercury) Metals (SPLP) Low-level hexavalent chromium Metals Platinum Group Metals Lead (Pb) in Air Perchlorate In-vitro bioassessibility assays for arsenic & lead in soil Water Ambient Air Water Dust wipes, Ghost wipes Soil, Sediment, Solid, Waste, Tissue Drinking Water Soil Catalytic converter washcoat TSP high-volume filters Water, Soil Soil Titration with Dichromate Cold Vapor Atomic Fluorescence CVAF (EPA 1630) ICP, ICPMS, CVAA SW8461312:ICP, GFAA, CVAA, ICP/MS 1C with post column reaction/ UV detection Portable XRF Portable XRF FEM EQL-0710-192, ICP/MS LC/MS/MS (EPA Method 331.0) EPA 9200. 1-86 Superfund Air, Water (TMDL) Water Tribal Program Superfund, RCRA Water Superfund, Criminal Investigation Enforcement, Air Air Superfund/Water Superfund New Pb NAAQS ORGANIC CHEMISTRY Diazinon 1 ,4-Dioxane EDB/DBCP Methane, Ethane, Ethene Water Water, Soil, Sediment Water Water ELISA GC/MS GC (EPA 504.1) GC/FID(RSK-175) WQM Superfund, RCRA Superfund, RCRA Superfund, RCRA BIOLOGY/MICROBIOLOGY Benthic Taxonomic Identification Chlorophyll/Pheophytin Enterococci Heterotrophic Bacteria Microcystin Toxicity Test, Red Abalone (Haliotis rufescens) Larval Development Toxicity Test, Sea Urchin Fertilization (Stronglyocentrotus purpuratus) Sediment (Marine) Water/Periphyton Water Water Water Water Water Taxonomic Identification Standard Method 1 0200 H, Procedure 2b Enterolert Plate Count - Standard Methods Immunoassay EPA/600/R-95/136 EPA/600/R-95/136 Water, WQM Water, WQM Water, NPDES, WQM Water, NPDES, WQM Water NPDES Water, NPDES ------- FY2014 Annual Report ll Regional Laboratories Unique Capabilities FY2014 REGION 10 Analyte/Group Name Sample Media Analytical Technique Supported Program(s) Comments INORGANIC CHEMISTRY Asbestos, Bulk Low-Level Mercury Methyl Mercury Metals Metals - Arsenic speciation Metals (TAL) + Total Uranium Metals (SPLP) Chlorophyll a In-vitro Bioassessibility Assays for Lead in Soil Percent Water Perchlorate Solids Water EPA600/R93/116-XRD CVAF, Method 1 631 E Water GC/CVAFS, Method 1630 Air filters Blood Soil Paint Solid Fish/shellfish/seaweed Small mammals, invertebrates Soil/Waste Water Soil Liquid Waste Produce (fruits, milk) ICP/MS, ICP ICP/MS Portable XRF Portable XRF X-Ray Diffractometer (XRD) IC/ICP/MS Microwave Digestion, ICP/ AES, ICP/MS ICP/AES, ICP/MS SM1002H Leachates by Method 1340, ICP/AES Karl Fischer titration IC/MS Superfund Water, Superfund Water, Superfund CAA Superfund Superfund, Criminal TSCA, Criminal Superfund Superfund, Water Superfund, RCRA Superfund Water Superfund RCRA Superfund 0.2 to 0.5 ng/L reporting limits Screening results for metals Lead in paint Characterizes the form metals exist in sample Speciation data needed for risk assessment Biomonitoring projects ORGANIC CHEMISTRY BNA (selected) Butyl tins 1 ,4-Dioxane Explosives (Nitroaromatics & Nitroamines) Hydrocarbon Identification N-Nitrosodimethylamine Herbicides Polybrominated diphenyl ethers (PBDEs) Total Petroleum Hydrocarbons- Gasoline Range Organics VOA & SVGA Low-level Polyaromatic Hydrocarbons and Other Neutral Organics Low-level Polyaromatic Hydrocarbons Tissue Soil/Sediment Water Water, Soil, fish/ shellfish Water, Soil/Sediment Water, Soil Water, Soil/Sediment Water Sediment/bio solids Tissue (fish) Water, Soil Water, Soil Industrial wastes, Solids, Tissues Soil, Sediments Shellfish, Water SW846 Methods Superfund GC/MS Superfund, Criminal EPA Method 8270D SIM/ Method 522 EPA Method 8330/HPLC NWTPH-HCID Method 521 GC/MS GC/MS Low Resolution GC/MS Low Resolution GC/MS Low Resolution NWTPH-Gx NWTPH-Dx Vacuum distillation, Method 8261 A GC/MS-MS GC/MS-MS Superfund Superfund Superfund, Criminal Superfund Superfund Water Superfund, Water Superfund Superfund, RCRA Superfund, RCRA Superfund, RCRA Superfund, Brownfields, Water Superfund, Brownfields WDOE method PHYSICAL AND OTHER DETERMINATIONS Multi-Increment Sampling (MIS) Preparation of Soil Samples for Organic & Inorganic Analyses Variety of water quality tests Soil Water Described in Method 8330B Appendix Various probe-type measurements Superfund Superfund Flow thru cell system; performed in the field BIOLOGY/MICROBIOLOGY Aeromonas spp Cryptosporidium & Giardia Enterococci Microbial Source Tracking Microscopic testing Drinking Water Water Ambient Water Water Drinking/Source Water EPA Method 1605 EPA Method 1623 (Filtration/ IMS Staining) EPA Method 1600 PCR Microscopic particulate analysis SDWA- Unregulated Contaminant Monitoring Rue (UCMR) SDWA, Water, Ambient Monitoring Rule - recreational waters Ambient Monitoring Rule Water Surface Water Treatment Rule EPA Approved On approval list for LT-2 regulation Microscopic technique used to establish GWUDI characteristics of a drinking water ------- FY2014 Annual Report Regional Laboratories Developing Capabilities FY2014 REGION 1 Project Method Enterococcus in Water by qPCR (EPA Method 1611) Capability Development Developmental Need Water Status In progress Projected Completion FY2014 REGION 2 SIM Analysis for VOA& Semi-VOA analysis MST Markers, Pig, Cow, Gull PCPP Hospital Waste Drinking & Surface Water Stormwater Emergency Contamination Developing methods on current instrumentation Completed Completed FY2015 REGION 3 Arsenic Speciation for Water, Soil/ Sediment, & Tissue by 1C or ICP/MS EPA Method 1694 for Pharmaceuticals & Personal Care Products by LC/MS/ MS PCR Quantitation & Source Tracking Glycols in Water ELISA Independent Laboratories Institute Solid-Phase Extraction Study Speciation data to be used for Risk Assessments in support of Clean Water Act & Superfund Need for capability to identify & quantify pharmaceutical & personal care products Need for capability to determine source of E. coli contamination in support of Water Program Need for capability to identify glycol compounds in groundwater using LC/MS/MS to achieve lower quantitation limits Need for in-field testing of surface & drinking water for presence of estrogen & estrogen-like compounds Office of Water Identified developmental need; initiated research & evaluation of analytical procedures; project placed on hold due to lack of demand for analysis Cancelled due to loss of analyst In-progress Complete Complete Continued work On Hold On Hold FY2013 FY2013 FY2013 REGION 4 EPA Method 8261 Internal Method - GC/MS/MS Pesticides by Method 8081 , DDT Suite Pesticides by Method 8270D using GC/ MS/MS Low-level TO-1 5 using Selective Ion Monitoring simultaneous Full Scan Hydride ESI Autosamplerfor high matrix samples and lower reporting limits for arsenic and selenium Release of silica and other metals from nanoparticle study Bioavailability of lead VOCs in difficult matrices Low-level Pesticides w/MS confirm Superfund Superfund Superfund, Air ODMDS, Superfund, TMDL ORD Superfund Initial investigation ITMEs in-process Paper was published 2014 Continued Work Continued Work Continued Work Continued Work Continued Work Unknown January 2015 REGION 5 PFOA/PFOS in Biosolids & Water qPCR, Gene Sequencing Guar Gum Methane, Ethane, & Ethene in Water by GC/FID Fluorotelemer Alcohols in Water by LC/ MS/MS Standard Operating Procedure for Analysis of (Tri-n-butyl)-n tetradecylphosphonium chloride (TTPC) in Soil by Multiple Reaction Monitoring Liquid Chromatography/ Mass Spectrometry (LC/MS/MS) PFOA/PFOS in Biosolids PFOA/PFOS in Water Methane, Ethane and Ethene in Water qPCR Gene Sequencing Guar Gum Fluorotelemer Alcohols in Water by LC/ MS/MS Water Division study - RMI HF fluid screening tool - Region 3 support Water Program request Water SF Emergency Response Water Water Water Water, HF Water Initial work done, new instrument installed & standards run to set up instrument. Some samples sequenced; screening tool in process Method developed; SOP in draft Initiated Initiated and Completed Completed Completed Continued work Continued work Continued work FY2015 FY2015 FY2015 FY2015 EPA 910-R-15-002 ------- FY2014 Annual Report ll Regional Laboratories Developing Capabilities FY2014 REGION 6 Project Method Anions and Oxyhalides by 1C Asbestos Alcohols by Headspace GC/MS Analysis Dissolved Gasses in Water by GC/FID Direct mercury analysis (CVAF - milestone) PAHs by GC/QQQ Dinitrotoluene minor isomers High Dissolved Solids Modified Method/Anion High Dissolved Solids/Modified Method/Cation High Dissolved Solids/Modified Method/OA PPCP analysis Passive Formaldehyde Induction-Coupled Plasma Axial Method Cyanide in Soil Matrix Sulfide in Water Matrix Low Molecular Weight Acids in Resource Extraction Analysis Haloacetic Acids in Resource Extraction Analysis PAHs by GC/QQQ Dinitrotoluene minor isomers High Dissolved Solids/Modified Method/Anion High Dissolved Solids / Modified Method/Cation High Dissolved Solids/Modified Method/OA PPCP analysis Passive Formaldehyde Induction Coupled Plasma Axial Method Cyanide in Soil Matrix Sulfide in Water Matrix Low Molecular Weight Acids in Resource Extraction Analysis Haloacetic Acids in Resource Extraction Analysis Developmental Need Remove dependence on State Lab for this test Superfund/RCRA Enforcement Energy Extraction Energy Extraction Clean Water Act, RCRA, Superfund RCRA, Superfund Superfund Clean Water Act, RCRA, Superfund Clean Water Act, RCRA, Superfund Clean Water Act, RCRA, Superfund Water Clean Air Act Superfund; new technique to generate lower reporting limits for metals RCRA & Superfund RCRA & Superfund Drinking Water Drinking Water Superfund, RCRA Superfund Superfund, RCRA, CWA Superfund, RCRA, CWA Superfund, RCRA, CWA CWA CAA Superfund Superfund, RCRA Superfund, RCRA SDWA SDWA Status Method developed, need DOC/MDL; SO Ps Training; DOC; SOP preparation Completed during FY2013 Completed during FY2013 DOC/MDL; SOP preparation Continued method development Continued method development Method being developed Method being developed Method being developed Method being developed Method being developed Method being developed; performance studies are ongoing Method being developed Initiated method development Method being developed Method being developed Completed Completed Continued Continued Continued Continued Continued Continued Completed Completed Continued Continued Projected Completion September 201 4 Program-dependent December 201 3 December 201 3 December 201 4 December 2014 December 201 4 October 201 4 October 201 4 October 201 4 April 201 4 Summer 2014 FY2014 FY2014 FY2014 Spring 2015 FY2014 EPA 910-R-15-002 ------- FY2014 Annual Report Regional Laboratories Developing Capabilities FY2014 REGION 7 Project Method EPA Method 1694 for Pharmaceuticals & Personal Care Products by HPLC/ MS/MS Pesticides by GC/MS/MS Microbial Source Tracking Using qPCR Arsenic Speciation for Water, Soil/ Sediment & Tissue by 1C or ICP/MS EPA Method 1 694 for Pharmaceuticals & Personal Care Products by HPLC/ MS/MS PAH/SVOC in Water by Stir Bar Sorbtive Extraction Airborne VOC by solid sorbent tube (EPA Method TO-1 7) Arsenic Speciation for Water, Soil/ Sediment & Tissue by 1C or ICP/MS Developmental Need Speciation data to be used for Risk Assessments in support of Clean Water Act & Superfund Confirmational analysis of pesticide analytes previously performed by GC/ECD TMDL & Stormwater Speciation data to be used for Risk Assessments in support of Clean Water Act & Superfund Water Water, SUPR Water, SUPR, RCRA Water Status Performing method validation studies on surrogate compounds; developing SOP Instrument installed, method development & validation pending Non-human marker test completed; pending additional technical method guidance from ORD Method development currently underway Continued work Continued work Continued work continuing Projected Completion FY2015 FY2016 FY2015 FY2015 REGION 8 Asbestos/Electron Microscope Endocrine Disrupter Studies/LC/MS/MS Macroinvertebrate - Freshwater Benthic/Manual Enumeration Microbial Source Tracking Microbial Source Tracking by PCR Arsenic Speciation for Water, Soil/ Sediment, &Tissue/IC/ICP/MS Toxicity - Acute & Chronic in Mobile Lab Pharmaceuticals by LC/MS/MS Pesticides by LC/MS/MS Hormones & Steroids by LC/MS/MS Algal Toxins Need for capabilities to analyze water & soils for asbestos contamination at Superfund sites Emerging needs for the Water program & ORD Redevelop capability for Water program support due to loss of staff Develop capabilities in this technology for use in projects & emerging needs for the Superfund, Water programs, & ORD Develop capabilities in this technology for use in projects and emerging needs for the Water, Enforcement programs and ORD. Speciation data to be used for Risk Assessments in support of Clean Water Act & Superfund Onsite assessment for potential needs by the Water program Water& ORD Water Water& ORD Water Instrument operational & running samples Performing method validation Planning to hire replacement staff Biolog system installed; some staff trained; assessing method Instruments & sample processing, ESAT staff training and/or assessing methods Identified developmental need; initiated research & evaluation of analytical procedures; necessary modifications to laboratory in progress Mobile lab available; team lead initiating discussion of projects & team development Progress continuing Progress continuing Progress continuing continuing Ongoing Ongoing Ongoing Ongoing Ongoing Ongoing Ongoing Ongoing Ongoing Ongoing REGION 9 Low-level total mercury in water (EPA 1 631 E) Analysis of Radiello passive air samplers for VOCs Anatoxin-a analysis using Receptor Binding Assay (RBA) Analysis of lead (Pb) on Teflon PM2 5 Filters Methyl Mercury in Environmental Samples Address regional priority Address regional priority Water Program monitoring for cyanotoxins Air Water Instrumentation installed; method development initiated Assessing health & safety issues Method development Initiated; no new equipment needed; SOP drafted Continued Completed FY2015 FY2015 EarlyFY2016 EPA 910-R-15-002 ------- FY2014 Annual Report ll Regional Laboratories Developing Capabilities FY2014 REGION 10 Project Method Develop Methyl Mercury Analysis Capability for Sediment Samples EPA Method 8330B Marine Tissue Method Evaluation/Development Ultra-trace Concentration Phosphorus Method for Treated Wastewater Effluent & Surface Water Low-level Polynuclear Aromatic Hydrocarbons (PAHs), Phenolics, & Other Neutral Organics in Soils & Sediments Low-level PAH Analyses of Waters & Clam Tissues EPA Method 8330B Marine Tissue Method Evaluation/Development-Multi- Lab Study Low Level Polynuclear Aromatic Hydrocarbons, Phenolics and Other Neutral Organics Analyses of Soil and Sediments Low Level Polynuclear Aromatic Hydrocarbons Analyses of Waters and Clam Tissues Develop Methyl Mercury Analysis Capability for Sediment Samples Ultra-trace Concentration Phosphorus Method for Treated Wastewater Effluent and Surface Water Developmental Need Methyl mercury data needed to support regional mercury strategy toward characterizing levels in the environment & evaluate public health risks Explosive concentration data in marine tissue samples are needed to help evaluate marine areas polluted with military munitions NPDES compliance monitoring at ultra-low phosphorus levels Measure PAHs & other semi- volatile neutral organic compounds at low concentrations in marine sediments to assess against NW states cleanup standards with organic carbon normalization Measure PAHs at low levels in clam tissues to support risk assessment activities Superfund Superfund, Brownfields, Water Superfund, Brownfields, Water Superfund, Water Water Status Some initial testing on instrument conducted; based on the effort needed to develop the water method, capability for sediment analyses will likely require much experimentation with the Brooks-Rand instrument to acquire the needed accuracy & sensitivity for sediments Method development Completed; multi-laboratory study through the QATS contract is in progress Ultra-trace standard concentration measurements were achieved on a Lachat colorimetric instrument & an ICP/MS system; testing on actual effluent samples still to be planned GC/MS/MS system was setup for extract analyses at very low concentrations Low-level Polynuclear Aromatic Hydrocarbons Analyses of Waters & Clam Tissues Completed Completed Completed Continued work Continued work Projected Completion FY2015 Completed FY2014 FY2015 Developed and Completed FY2014 Developed and Completed FY2014 ------- FY2014 Annual Report Appendix A Acronyms/Abbreviations &EPA United States Environmental Protection Agency EPA 910-R-15-002 ------- FY2014 Annual Report Acronyms/Abbreviations ACIL ADEM AQS ASTM BMP BNA BOD CAA CDC CENWP CERCLA CFR CID CLP COD Cr CVAA CWA DBCP DECA DNT EDB EPA ESAT FDA FY GC GC/ECD GC/FID GC/MS GC/MS/MS GC/NPD GFAA Hg HPLC/UV 1C ID -A- American Council of Independent Laboratories Alabama Department of Environmental Management Air Quality System American Society for Testing and Materials -B- best management practice base/neutrals and acids biological oxygen demand -C- Clean Air Act Centers for Disease Control Corps of Engineers' Portland District Comprehensive Environmental Response, Compensation, and Liability Act Code of Federal Regulations Criminal Investigation Division Contract Laboratory Program chemical oxygen demand Chromium cold vapor atomic absorption Clean Water Act -D- Dibromochloropropane Division of Enforcement and Compliance Assistance dinitrotoluene -E- ethylene dibromide Environmental Protection Agency Environmental Services Assistance Team -F- U.S. Food and Drug Administration Fiscal Year -G- gas chromatography gas chromatography/electron capture detector gas chromatography/flame ionization detector gas chromatography/mass spectrometry gas chromatography/mass spectrometry/mass spectrometry gas chromatography/nitrogen-phosphorous detector graphic furnace atomic absorption -H- Mercury high performance liquid chromatography/ultraviolet -1- ion chromatography identification EPA 910-R-15-002 ------- FY2014 Annual Report Acronyms/Abbreviations ICP ICP/AES ICP/MS III ISE ISO LC/MS LC/MS/MS LHC LTIG MCHM MCR MeHg MPRSA NEIC NELAC NGO NO2 N03 MRS A ODMDS ORCR ORD OSC OSWER OU PAH PC PCB PCE PEP PFC PM -I- Inductively-coupled plasma Inductively-coupled plasma/atomic emission spectrometry Inductively-coupled plasma/mass spectrometry Independent Laboratories Institute ion selective electrode International Standards Organization -J- -K- -L- liquid chromatography/mass spectrometry liquid chromatography/dual mass spectrometry light hydrocarbon Laboratory Technical Information Group -M- 4-methylcyclohexanemethanol Mouth of the Columbia River methylmercury Marine Protection, Research, and Sanctuaries Act -N- National Enforcement Investigations Center National Environmental Laboratory Accreditation Conference Non-Governmental Organizations nitrite nitrate National Rivers and Streams Assessment -O- Ocean-Dredged Material Disposal Site Office of Resource Conservation and Recovery Office of Research and Development On-Scene Coordinator Office of Solid Waste and Emergency Response Operable Unit -P- polycyclic aromatic hydrocarbon plate count polychlorinated biphenyl tetrachloroethylene Performance Evaluation Program perfluorinated chemical particu late matter EPA 910-R-15-002 ------- FY2014 Annual Report Acronyms/Abbreviations PNW PPCP PPH QAPP QC QuEChERS R2P2 RCRA RLN RMN SCAS SIMS SIP SLAMS SOP SPE SRB STP TCE TCLP TMDL TOC TPH TTPC USAGE U.S. EPA uses VOA VOC -P- Pacific Northwest Pharmaceuticals and Personal Care Product polyglycol ethers -Q- Quality Assurance Project Plan Quality Control Quick, Easy, Cheap, Effective, Rugged, Safe -R- Regional Research Partnership Program Resource Conservation and Recovery Act Regional Laboratory Network Regional Monitoring Network -S- semi-continuous activated sludge secondary ion mass spectrometry state implementation plan State and Local Air Monitoring Station standard operating procedure solid-phase extraction sulfate-reducing bacteria sewage treatment plant -T- trichloroethylene toxicity characteristic leaching procedure total maximum daily load total organic carbon total petroleum hydrocarbon (Tri-n-butyl)-n-tetradecylphosphoniumchloride -U- U.S. Army Corps of Engineers United States Environmental Protection Agency U.S. Geological Survey -V- volatile organic analysis volatile organic compound -W- -X- -Y- -Z- EPA 910-R-15-002 ------- ------- ------- |