Macroinvertebrate Protocol and Development of the
Puerto Rico High Gradient Streams Benthic Index
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Report prepared by:	^ James Kurtenbach, Aquatic Biologist
Monitoring Operations Section
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Approved by:	Joh^S. Kushwara, Chief
Monitoring and Assessment Branch
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Table of Contents
List of Figures	ii
List of Tables	iii
Executive Summary	 	 	iv
Introduction	1
Methods		3
Results	10
Discussion	14
Conclusions	17
Literature Cited	18
Appendix A-1: Stream Sites and Locations
Appendix A-2: Water Chemistry Data
Appendix A-3: Physical Habitat Data
Appendix A-4: Watershed Forest Land Cover Data
Appendix A-5: Biological Metric Data
Appendix B-l: Candidate Metric Distributions
Appendix B-2: Index Alternatives
Appendix C-l: Statistical Correlations Between Metrics and Water Chemistry Data
Appendix C-2: Statistical Correlations Between Metrics and Physical Habitat Data
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List of Figures
1.	Map of stream sites in Puerto Rico	4
2.	Scatter plot of elevation (m) and index score		 			14
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List of Tables
1.	List of measures evaluated for use as biological metrics and expected response of
metric to increasing stress	7
2.	Measures of water quality, physical habitat, and land-use for sites sampled during
1994, 2006, and 2009	8
3.	Spearman rank correlations (p<0.05) between potential metrics and water chemistry
variables for the 50 sites sampled in 2009	11
4.	Spearman rank correlations (p<0.05) between potential metrics and physical habitat
measures for 50 sites sampled in 2009	11
5.	Correlation coefficients (Pearson product-moment) among metrics	12
6.	List of final candidate metrics for development of a multimetric index	12
7.	Scoring of the metrics	12
8.	Index alternatives	13
9.	Stream parameters differing for reference verses test sites (* indicates significance at
p<0.05)	13
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Executive Summary
Under the Clean Water Act, States, US Territories, and Commonwealths are required to
measure status and trends of surface water quality, and determine the extent to which
waterbodies support healthy aquatic communities. To date, this has been accomplished
through monitoring programs designed to routinely monitor waterbodies for various
chemical, physical, and biological parameters. Benthic macroinvertebrates have a long
history of use as biological indicators of water quality. In temperate North America,
cost-effective bioassessment methods for benthic macroinvertebrates have been
developed and successfully implemented to measure environmental impacts and long-
term trends of environmental conditions. However, a similar level of technical
development of protocols is lacking on streams in the Caribbean and throughout the
neotropics. This knowledge gap is of particular concern in Puerto Rico, where aquatic
resources are threatened by widespread degradation caused by numerous anthropogenic
disturbances.
This report describes a stepwise process used to develop a multimetric index of biological
integrity that is responsive to environmental stresses and applicable to wadeable streams
in the interior region of Puerto Rico.
With the use of a traveling kick net, macroinvertebrate samples were collected from riffle
areas of 174 stream sites during the spring (March-April) of 1994, 2006, and 2009. Sites
were located in watersheds representing a wide range of land-use and human activity.
Macroinvertebrate samples collected were identified and enumerated. In addition, water
chemistry, physical habitat, and land-use data were collected and compiled for use in the
analysis of benthic macroinvertebrate data. Various measures (metrics) of taxa richness,
taxa composition, pollution tolerance, and functional feeding groups were screened for
their discriminatory power, relationship to stressors, and redundancy. Collectively, this
information was used to select a set of candidate metrics. Subsequently, a test of multiple
indices, with different combinations of metrics, was conducted to determine which index
performed the best. The outcome of this process resulted in the development of a
Macroinvertebrate Integrity Index (Mil).
The index recommended as the Mil includes the following metrics:
•	Taxa richness
•	Caddisfly richness
•	Percent coleoptera
•	Percent gastropoda
•	Percent diptera
•	Percent scrapers (excluding gastropoda)
•	Percent intolerants
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Individual metric scores (0-100) are combined and simply averaged to derive a
multimetric index score, A higher metric score is indicative of better biological
condition. Overall, metrics that comprise the Mil showed a moderate to strong response
across environmental gradients of land use, water chemistry, and physical habitat. Using
land use change as a surrogate for human disturbance, the Mil distinguished between
reference and impaired stream sites.
Few examples of studies from the neotropics exist in the documented literature on the
response of benthic macro i nvertebrates to pollution, particularly the use of rapid
bioassessment protocols (RBP's) and a multiple metric system of biological assessment.
Our study demonstrates the first known use of macroinvertebrate communities, collected
from Caribbean island streams using rapid bioassessment techniques, able to identify a
core group of biological measures, and applied to assess and monitor environmental
conditions. The Mil shows potential to provide accurate determination of aquatic life use
support, help develop biological criteria, and protect aquatic stream resources in Puerto
Rico.
Regardless of the study findings, additional information on the structure and function of
benthic macoinvertebrate assemblages in Puerto Rico streams is needed. Future studies
need to provide more information on species taxonomy, habitat use, pollution tolerances,
and functional feeding group assignments. With a further understanding of tropical
stream ecology and taxa life history requirements, the Mil may be refined to more
precisely and accurately measure the environmental quality of streams. This study shows
specific attributes of the benthic macroinvertebrate community incorporated into the Mil
are responsive to stresses that result from human disturbance. Nonetheless, additional
testing of the Mil is recommended to determine the geographic extent of use and to
examine its sensitivity to specific groups of pollutants and multiple stressor gradients
(e.g., nutrients, toxics, habitat) and have their variability examined.
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Introduction
Aquatic resources in Puerto Rico are threatened by continued degradation caused by
numerous anthropogenic stressors. Human population growth on Puerto Rico has
increased by 86% since 1940, was at 3.5 million people in 1990, and currently stands at 4
million. Expanding human population, rapid economic development, unplanned
development and lags in infrastructure and pollution control have resulted in a water
quality crisis. Water pollution represents one of the most significant environmental
problems in Puerto Rico (Hunter and Arbona, 1995). Streams in Puerto Rico face
significant ecological risks from improperly treated sewage, landfill contaminants, water
diversions, extensive land conversion, and deforestation.
The mountains known as the Luquillo, Cayey, Bermeja and Cordillera, extend across the
interior region of Puerto Rico. Aquatic resources of this area include streams and rivers,
reservoirs, and wetlands. Federal and Commonwealth agencies routinely monitor these
waterbodies for various chemical, physical, and biological parameters. In practice,
measurements of these parameters should enable agencies to determine whether they are
meeting the goals of the Clean Water Act. Objectives stated in Section 101 of the Clean
Water Act are "to restore and maintain the chemical, physical, biological integrity of the
Nation's waters." Currently, a number of assessment tools are being used or proposed for
monitoring surface water quality. Unfortunately, there is substantial controversy
regarding the present ability of monitoring programs to document water quality
improvements or declines on a regional and national scale (USEPA 2003, Shapiro et al.
2008). In response to this concern, several recommendations have been made to enhance
surface water monitoring, including the application and development of promising
biological techniques and statistical survey designs (USEPA 1987, ITFM 1995, Olsen
and Peck 2008). With an outgrowth of these recommendations and a renewed interest in
biological assessments, EPA has developed rapid bioassessment protocols (RBP's) and
technical guidance on biological criteria for use on streams and rivers (Plafkin et al.
1989; Gibson et al. 1996; Barbour et al. 1999). RBP's are a synthesis of existing
methods, and designed as inexpensive screening tools for determining biological
conditions. In contrast to temperate North America, where RBP's have been developed
and successfully implemented to measure environmental conditions, a similar level of
development is lacking on streams in the Caribbean.
Benthic macroinvertebrate sampling was conducted by EPA Region 2, March 1994, at
105 stream sites located in the mountains of the eastern, central, and western northern
slope drainages of Puerto Rico. Sampling sites were generally located upstream of bridge
crossings or adjacent to road access points. The number of sites selected in a major river
basin was proportional to the area of the watershed. Benthic macroinvertebrates were
captured from rock substrates using a rectangular kick net (Bode 1991). In addition
water quality and physical habitat data were collected. Streams ranged in size from
second to fourth order. In this study the RBP II (family level taxonomy) was applied and
tested, and correlation analysis was used to determine the degree of association among
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several variables of macroinvertebrate, physical habitat, and water quality data. Prior to
this study, a rigorous analysis of the relationship of biological assessment to
environmental quality in Puerto Rico streams had not occurred.
A second round of analysis was performed on the same dataset using genus/species
taxonomy and results once again showed that associations between the biological metrics
and water quality and physical habitat were weak. However, elevation had a moderately
strong association with several of the biological metrics tested. This finding did not
provide conclusive information supporting the idea that elevation is important, because
more human disturbance from residential development is found at lower elevations.
Nonetheless, elevation was identified as a potentially important source of natural
variability that needed to be addressed. One recommendation from the original study was
to determine the effects of elevation only at sites representative of reference conditions.
Subsequently, an additional sampling of benthic macro invertebrates was conducted in
March-April 2006. at 19 stream sites located in the eastern, central and western mountain
drainages of Puerto Rico. A statistical survey design was used to randomly select stream
sites that had greater than 85% forest cover in 1950 and currently have a catchment area
upstream with a minimum of 85% forest cover. These sites were judged to be of
reference quality. Reference is defined here as a least disturbed condition found in
conjunction with the best available physical, chemical, and biological habitat given
today's state of the landscape (Stoddard et al. 2006). Results of the study suggested the
range of elevation studied did not influence most of the biological measures (Kurtenbach
2008). Study of other neotropical streams have shown mixed results on the relationship
between elevation and structure of macroinvertebrate communities (Harrison and Rankin
1976; Pringle and Ramirez 1998; March et al. 2002).
In addition, this study provided an opportunity to assess regional influences on biological
measures. Regional trends for some of the biological metrics were found (Kurtenbach
2008). Regional changes in the distribution and species composition of aquatic fauna are
known to occur with differences in climate, geology, hydrology, biogeography. and
landscape. Omernik (1987) used an integration of these factors to develop ecological
regions for the United States. Biological characteristics of streams have been shown to
correspond with ecoregions (Rohm et al. 1987; Whittier et al. 1988; Lyons 1989). In
neotropical streams regional patterns of community structure and function are not clearly
evident (Covich 1988). At the present time, no ecoregion or similar regional approaches
have been developed for Puerto Rico.
In March 2009, a probabilistic survey design was used to establish baseline
environmental conditions of wadeable streams across Puerto Rico. Water chemistry,
physical habitat, and macroinvertebrate samples were collected from 50 stream sites.
Methodology used for the field collection and lab processing of macroinvertebrate
samples was consistent with previous studies performed on Puerto Rico streams in 1994
and 2006. Data were examined to determine what percent of streams in Puerto Rico are
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in good, fair, and poor condition for key indicators of ecological health; and identify and
rank the relative importance of key chemical and physical stressors. Several water
chemical and physical habitat parameters showed associations with ecological attributes
of the macroinvertebrate assemblage. Results of the survey will help guide future
monitoring and stream management decisions in Puerto Rico.
Data used for the index development were a compilation of the survey years, 1994, 2006,
2009. respectively. These data included benthic macroinvertebrate samples, site and
watershed characteristics, physical habitat, and field water quality parameters.
Few examples of studies in the neotropics exist in the documented literature on the
response of benthic macroinvertebrates to pollution, particularly the use of RBP's and a
multimetric system of biological assessment (Thome and Williams 1997; Weigel et al.
2002; Buss and Vitorino 2010). Our study demonstrates the first known use of
macroinvertebrate communities, collected from Caribbean island streams using rapid
bioassessment techniques, ability to identify a core group of biological measures, and use
it to assess and monitor environmental conditions. The study provides critical
information on the natural factors affecting the macroinvertebrate assemblage in streams
of Puerto Rico, and also the development and application of biological assessments.
Methods
Study area
All benthic macroinvertebrate sampling was conducted in March-April over the survey
years (1994, 2006, and 2009), at a total of 174 stream sites located in the eastern, central
and western mountain drainages of Puerto Rico (Figure 1). The area of study included
three geographic regions, humid east central, rainy west central, and rainy Luquillo
Mountains, all characterized by relatively heavy rainfall and steep topography. Stream
sites and their associated latitude and longitude locations are listed in Appendix A-1.
Stream size ranged from 1 st to 4th order. Stream sites were exclusively high and
moderate in gradient and dominated by riffle-run habitat. The bottom substrate at all
sites was similar, consisting mostly of small boulder and cobble with lesser amounts of
gravel, sand, and silt. The catchment area upstream of each site had varied land use.
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Figure 1. Map of stream sites in Puerto Rico.
1994, 2006, 2009
Puerto Rico Biological Stream Survey's
- l".	'
e * *>•	• • s	•
• • m f *• •,
ml* %
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fc	¦	O	^ ¦ f - ®
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&EPA A c "
Stream Sites
• 1994
2006
Kilometers
=d	* 2009
Site selection
Sites selected for the 1994 stream survey were easily accessed from road bridge
crossings, while the total number of sites sampled was proportional to the size of the
drainage area. Sampling locations selected for the 2006 and 2009 surveys used a
probabilistic survey design (Olsen et al. 1999). Site selection did not include weighting in
the number of stream sites from 1st through 4th order stream classes. A set of reserve
sites was chosen and served as replacements when target sites were inaccessible due to
physical barriers or access was denied by the landowner.
Macroinvertebrate collection and preservation
At each stream site a single riffle area was chosen for the collection of a benthic
macroinvertebrate sample. Benthic macroinvertebrates were captured from rock
substrates (cobble and small boulder) using a large frame rectangular kick net,
constructed with an 800 x 900 «m mesh net (Bode 1991). Sample collection was
accomplished by placing the kick net on the stream bottom, mid-river in the riffle, while
gradually working the net downstream and laterally (approximately two net widths) for 5
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minutes. Bottom substrate was vigorously disturbed by foot so that sufficient organisms
would be dislodged and swept into the net.
After the sample was collected, large debris were removed from the net, inspected for
organisms, and discarded. Maeroinvertebrates and debris retained in the net were
removed and placed in a one liter container and preserved with 10% buffered formalin.
Sample containers were labeled with the appropriate site information and delivered to the
EPA Edison laboratory. Kick nets were thoroughly rinsed and cleaned between stream
sites.
Approach to index development
One underlying premise for the use of a biological index is that it is capable of detecting
impairment caused by water quality and habitat degradation. The multimetric index
described here was developed using benthic macroinvertebrate data and calibrated with
various environmental data collected over a range of anthropogenic influences.
Individual metrics used to comprise the index arc measures of community structure and
function, such as, richness, composition, pollution tolerance, and trophic feeding
measures. Development of the multimetric benthic index for Puerto Rico streams
generally followed those procedures described in EPA technical guidance (Gibson et al.
1996). Steps in this process (see chapter six) include a sequential progression listed as
follows: 1) select consistent biosurvev protocols, 2) collect and compile data, 3) stream
classification for regional expectations or reference condition, 4) metric selection and
calibration, 5) test of metrics discrimination ability, and 6) development of the index.
Data compilation
Data were collected over a March-April index period for three survey years (1994, 2006,
and 2009). In addition to the collection of benthic macroinvertebrate data, associated
water chemistry, physical habitat, and land use data were gathered (Appendices A 2-4).
Routine physical characterization (e.g., stream width, dominant land use, canopy cover,
and sediment deposits) and water quality measurements (e.g., pH, dissolved oxygen,
temperature, and specific conductance) of stream condition were recorded on field data
sheets for all three survey years. Land use cover was computed using remotely sensed
National Land Cover Data (NLCD) 2001 land cover data (Homer et al. 2007). To meet
the objects of the 2009 probabilistic streams survey, additional water quality (e.g., total
nutrients, major anions/cations, DOC, TSS, TOC, and turbidity) and physical habitat
(e.g., substrate, thalweg profile, instream fish habitat, and riparian vegetation cover)
measures were taken. All data were organized in Excel spreadsheets, including the
manipulation of the biological data (e.g., metric calculation and overall indexes).
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Identifying reference and impaired stream sites
Reference and stressed sites were selected based on specific land use criteria. To be
considered a reference site the upstream watershed needed greater than 85% forest cover
since the period of the 1950's. Criteria for the stressed sites was less than 30% forest
cover, with the remainder as some percentage of urban plus agriculture land use. It was
assumed that some degree of water quality and habitat impairment occurred along this
gradient of land use. Insufficient measures of water quality and physical habitat were
available to establish their use as screening criteria.
Metric evaluation
Measures of taxa richness, taxa composition, pollution tolerance, and trophic feeding
groups were evaluated as candidate metrics for analysis of the macroinvertebrate data
(Table 1). Metrics were reviewed and selected from a list of potential metrics described
in the literature (Barbour et al. 1999; Gibson et al. 1996). These are generally broad
based and adapted for temperate North America. Additional metrics were chosen to
reflect the composition of the macroinvertebrate assemblage found in streams of Puerto
Rico. Macroinvertebrate specimens were mostly identified to genus level using North
American keys and specific keys for the Caribbean (including Puerto Rico) and Meso
America. Distinct taxa were used for the calculation of the richness metrics.
Assignments of pollution intolerance were made for a few select taxa based on the North
American literature (Barbour et al. 1999), as this information is currently unavailable for
Puerto Rico. Generally these taxa occurred in families where most all of the genera are
considered pollution intolerant. Additionally, functional feeding group assignments were
obtained from the North American literature (Merritt and Cummins 1996, Barbour et al.
1999), and like pollution tolerance information, functional feeding group classification of
invertebrates is almost absent for Puerto Rico streams. Metrics were not considered if
there was a sparseness of data (e.g., too few taxa). A stepwise process was used to
eliminate metrics based on their discriminatory power, relationship with stressors, and
redundancy.
Discriminatory power of metrics was examined for the ability to distinguish between
reference and impaired streams. Using an approach adopted by Barbour et al. (1996),
box-and-whisker plot distributions based on the overlap of interquartile ranges provided a
visual assessment of the degree of overlap between reference and impaired streams.
Scores were assigned (0), entire overlap and (3) no overlap of the interquartile ranges.
Intermediate scores (1-2) represent some degree of overlap with either one or both
medians outside the other's interquartile range. Metrics with a score of 3 demonstrated
the ability to discriminate between reference and impaired streams, and these were
considered for additional analysis.
Metrics were evaluated to determine their relationships to specific stressors. Stressors
examined included measures of water quality, physical habitat, and land use (Table 2).
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Spearman rank correlations (p < 0.05) were used to detect significant stressor-metric
relationships. Metrics that showed multiple relationships with water physiochemical or
physical habitat parameters were retained.
Because metrics tend to respond to stressors differently, it is desired to have diverse
categories of metrics to effectively evaluate biological integrity. An index based on
metrics with a wide range of ecological attributes and sensitivity to disturbance is likely
to perform better than less robust indices. Redundancy was evaluated using a Pearson
Product-Moment correlation analysis. Metrics were reviewed for redundancy to ensure
each metric provides new information. Criteria for the rejection of redundant metrics was
a correlation of (r > 0.8), as this is generally considered the upper level of acceptability.
Table 1. List of measures evaluated for use as biological metrics and expected response
of metrics to increasing stress.
TAX A RICHNESS	STRESS
CFLYRICHN Trichoptera taxa richness	D
CRUSRICH Crustacean richness	D
ETRICH	Ephemeroptera and trichoptera taxa richness	D
TAXARICH Total taxa richness	D
TOTFAM	Total family taxa richness	D
TAXA COMPOSITION
PRCNTCFCM % Trichoptera	D
PRCNTCHIR % Chironomidae	I
PRCNTCOL % Coleoptera	D
PRCNTCRUS % Crustacea	D
PRCNTDIP % Diptera	I
PRCNTDOMI % Dominant taxon	I
PRCNTET % Ephemeroptera and trichoptera	D
PRCNTGAS % Gastropoda	I
PRCNTLEP % Lepidoptera	D
PRCNTMFCM % Ephemeroptera	D
PRCNTNINS % Non-insect composition	I
PRCNTODON % Odonata	D
PRCNTOLIG % Oligochaeta	I
POLLUTION TOLERANCE
%
PRCNTITOL Intolerant |	D
TROPHIC FEEDING
PRCNTGF % Collectors/Filterers	D
PRCNTPRD % Predators	D
PRCNTSCR % Scrapers	D
D	Decrease
I	Increase
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Table 2. Measures of water quality, physical habitat, and land use for sites sampled
during 1994, 2006, and 2009.
INSITU WATER CHEM
COND
Conductivity (uS/cm @ 25 C)
DO (mg/l)
Dissolved Oxygen
pH
pH
TEMP
Tempearture
WATER QUALITY
ATTL
ALKALINITY, TOT_PPM
NH3
Ammonia (As N)
CA
Calcium (pg/L)
CL
Chloride (ueq/L)
CL_PPM
Chloride (mg/L)
COND
Conductivity (uS/cm @ 25 C)
DO (mg/l)
Dissolved Oxygen
DOC
Dissolved Organic Carbon (mg/L)
K
Potassium (|jg/L)
MG
Magnesium (jjg/L)
NA
Sodium (pg/L)
N03-
Nitrate (mg/L)
N02-
Nitrite (as N)
N03N_PPM
Nitrate Nitrogen
NTL
Total Nitrogen (mg/L)
PH
pH
PTL_PPM
Total Phosphorus (mg/L)
P
Orthophosphate (as P)
RNF
Residue, non-filterable
SI02
Silica (mg/L Si02)
S04
Sulfate (mg/L)
TEMP
Tempearture
TOC
Total Organic Carbon (mg/L)
TSS
Total Suspended Solids (mg/l)
TURB
Turbidity (NTU)
PHYSICAL HABITAT
PCT_FN	% Fine
PCT_SA	Substrate % Sand (0.6-2mm)
XC	Riparian canopy cover (XCL +XCS)
XCDENBK	Mean % canopy density at bank
XCDENMID	Mean % canopy density mid-stream
XCEMBED	Substrate mean embeddness -- mid- channel (%)
XCL	Riparian canopy (> 5m high) cover -trees > 0.3m DBH
XCM	Riparian canopy +mid-layer cover (XC+XM)
XCMG	Riparian cover, sum of 3 layers (XC+XM +XG)
XCMGW	Riparian woody cover, sum of 3 layers (XC+XMW +XGW)
XCMW	Riparian canopy +mid-layer woody cover (XC+XMW)
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xcs
Riparian canopy (> 5m high) cover -trees > or equal to 0.3m DBH
XEMBED
Substrate mean embeddness -channel + margin(%)
XFC_ALG
Filamentous algae areal cover
XFC_ALL
Sum of areal cover from all fish concealment types except algae and aquatic macrophytes
XFC_AQM
Aquatic macrophyte areal cover
XFC_BIG
Sum of cover from large wood, boulders, over-hanging banks and human structures
XFC_BRS
Brush and small woody debris areal cover
XFC_HUM
Artifical structure areal cover
XFC_LWD
Large woody debris areal cover
XFC_NAT
Sum of cover from large wood, brush, overhanging vegetation, boulders and undercut banks
XFC_OHV
Overhanging vegetation areal cover
XFC_RCK
Boulder and rock ledge areal cover
XFCJJCB
Undercut bank areal cover
XG
Riparian ground-layer vegetation cover (XGW+XGH)
XGB
Riparian ground-layer (<0.5m high) bare ground cover
XGH
Riparian ground layer (<0.5m high) herbaceous cover
XGW
Riparian ground layer (<0.5m high) woody cover
XM
Riparian mid-layer cover (XMW+XMH)
XMH
Riparian mid-layer (0.5 to 5m) herbaceous cover
XMW
Riparian mid-layer (0.5 to 5m high) woody cover
W1_HALL
Riparian human disturbance index (all types) - High (H), Medium (M), Low (L)
LAND

COVER

%_Fo_90
Percent Forest (1994)
PFOR
Percent Forest (2006, 2009)
Scoring of the metrics
After filtering metrics for their discriminatory power, relationships to stressors, and
redundancy, scoring of the remaining metrics was performed. The distribution of
reference conditions was used as the benchmark for setting thresholds. The 95th and 5th
percentiles of maximum and minimum reference values were used to avoid inclusion of
anomalously high and low data outliers. Metric scores were calculated using the
formula's (metric value/95th percentile value) and (max - metric value)/(max - 5th
percentile value) for metrics that increase and decrease with response to stress. This
method of scoring metrics was considered the most sound and effective of alternatives in
use for the development of multimetric indexes (Blocksom 2003).
Final selection of metrics and index composition
Aggregation of the metrics into a final multimetric index was done to provide a means of
measuring biological impairment of the benthic macroinvertebrate community.
Procedures for development of the multimetric index were patterned after the approach
detailed in the rapid bioassessment protocols (Barbour et al., 1999) and biocriteria
technical guidance for streams and small rivers (Gibson et al.. 1996). The goal of this
process was to identify the most robust metrics (approximately 6 to 8) that effectively
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assess the biological conditions of Puerto Rico streams. A test of multiple indices with
different combinations of metrics was conducted to determine which index performed the
best. This was accomplished with box-whisker-plots used to determine which index best
distinguished reference and impaired stream sites. Several alternative indexes were
calculated using combinations of metrics deemed to perform the best based on
aforementioned criteria for selection of ecological meaningful and responsive metrics.
Individual metric scores (0-100) were combined and simply averaged to get a multimetric
index value. By standardizing each metric on a scale of 0-100, all metrics contribute
equal weighting to the combined index. A higher metric score is indicative of better
biological condition. Use of a continuous scoring scale requires setting thresholds for
categories of biological condition. This can be viewed as setting the upper and lower
range of values that indicate good, fair, and poor biological condition. The approach
used is similar to the percent range of deviation from the reference condition, as proposed
by EPA's National Wadeable Streams and Lakes Assessments (US HP A 2006, USEPA
2010).
Data used in the analysis of the index alternatives were collected over different survey
years and at multiple stream sites throughout Puerto Rico. To ensure the responsiveness
of the indexes was not due to natural variability; natural factors such as stream size,
elevation, and water temperature were examined. Measurement of the stream width was
used as a surrogate for stream size. Information on elevation was obtained from 1:24,000
USGS topographical maps. Stream temperature was measured in the field at the time of
the macroinvertebrate sampling.
Results
Metric evaluation
Three of the metrics (% gastropoda, % non-insects, and % intolerants) distinguished
between reference and impaired stream sites (score = 3), and the remainder of the metrics
evaluated had some degree of overlap with scores ranging from 0 to 2 (Appendix B).
Several of the metrics were highly correlated with water chemistry and habitat variables
(Appendices CI and C2). Taxa richness, caddisfly richness, % coleopteran, % caddisfly,
% lepidoptera, % scraper, and % col lector-filterer were all correlated with 5-7 water
chemistry variables (dissolved oxygen, chloride, TOC, DOC, suspended solids, turbidity,
and ammonia) that often are associated with organically rich effluents (Table 3), Metrics
that were significantly correlated with measures in two or three of the major habitat
categories (e.g., fish cover, in-stream sediment, riparian cover) included: caddisfly
richness, % gastropoda, % intolerants, % scraper and % collector-filterer (Table 4).
Several pairs of metrics were found highly correlated (r >0.80) and redundant of each
other (Table 5). Three of the metrics, taxa richness, caddisfly richness, and % diptera
were included in the final index and were not redundant with other metrics in the index.
Collectively, tests of discriminatory power, relationships to stressors, and redundancy,
were used to identify a list of final candidate metrics for the development of a
multimetric index (Table 6).
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Table 3. Spearman rank correlations (p<0.05) between potential metrics and water
chemistry variables for the 50 sites sampled in 2009.
Metric
Dissolved
Chloride
TOC DOC
Suspended Turbidity Ammo

Oxygen


Solids
Taxa
0.330
-0.268
-0.336 -0.310
-0.331 -0.339
Richness




Caddisfly
0.506
-0.485
-0.579 -0.536
-0.350 -0.316 -0.431
Richness




%
0.559
-0.364
-0.519 -0.457
-0.322 -0.368
Coleoptera




%
0.370
-0.426
-0.549 -0.501
-0.268 -0.384
Caddisfly




%
0.412
-0.385
-0.279 -0.308
-0.484
Lepidoptera




% Scraper
0.499
-0.540
-0.576 -0.591
-0.480 -0.355 -0.357
%
0.438
-0.368
-0.509 -0.494
-0.293
Collector-
filterer
Table 4. Spearman rank correlations (p<0.05) between potential metrics and physical
habitat measurements for the 50 sites sampled in 2009.
Metric
Caddisfly
Richness
%
Gastropoda
%
Intolerants
% Scraper
% Colleetor-
filterer
Filamentous
Algae Cover
-0.306
0.440
-0.367
Artificial
Structure
Cover
-0.384
-0.396
Substrate Riparian
Embeddness Canopy
-0.289
-0.304
-0.307
-0.338
Cover
-0,493
0.297
0.345
% Overhead
Canopy
-0.412
-0.331
11

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Table 5. Correlation coefficients (Pearson product-moment) among metrics.

TAXARICH
ETRICH
CFLYRICH
PRCNTMFCM
PRCNTCHIR
PRCNTCF
TOTFAM
0.949
0.815




TAXARICH

0.841




ETRICH


0.916



PRCNTET



0.883


PRCNTDIP




0.924

PRCNTCFCM





0.820
Table 6. List of final candidate metrics for development of a multimetric index.
_____
PRCNTCOL
PRCNTGAS
PRCNTDIP
TAXARICH
PRCNTCF
PRCNTSCR
PRCNTCFCM
PRCNTITOL	
Scoring of the metrics
Metric scoring formulas, including the rounded 95th and 5th percentile values are found in
(Table 7).
Table 7. Scoring of the metrics.
a	rHcth
Metric
95th percentile
5th percentile
Equations for scores
CFLYRICH
8

Metric value/8 x
100
PRCNTCOL
55

Metric value/55 x
100
PRCNTGAS

0
100-metric
value/100 -Ox 100
PRCNTDIP

3
100 - metric
value/100 -0 x 100
TAXARICH
18

Metric value/18 x
100
PRCNTCF
49

Metric valuc/49 x
100
PRCNTSCR
28

Metric value/28 x
100
PRCNTCFCM
56

Metric value/56 x
100
PRCNTITOL
19

Metric value/19 x
100
12

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Final selection of metrics and index composition
Nine metrics were considered final candidates for testing nine alternative indexes (Table
8), Index 2 demonstrated the greatest ability to distinguish between reference and test
stream sites, followed closely by indexes 4 and 6, and considerably less for the remaining
indexes (Appendix B2). Index 9 was tested by adding percent intolerant taxa to the suite
of metrics that comprised index 2. The addition of this metric slightly improved
discrimination between reference and test sites, and provided another metric that has
important ecological community information.
Table 8. Index alternatives.
Metric
Index1
Index2
Index3
Index4
IndexS
Index6
Index7
Index8
Index9
CFLYRICH
X
X
X
X
X
X
X
X
X
PRCNTCOL
X
X


X
X


X
PRCNTGAS
X
X
X
X
X
X
X
X
X
PRCNTDIP
X
X
X
X




X
TAXARICH
X
X
X
X
X
X
X
X
X
PRCNTCF
X

X

X

X


PRCNTSCR

X

X

X

X
X
PRCNTCFCM


X
X


X
X

PRCNTITOL




X
X
X
X
X
The index recommended includes the following metrics:
•	Caddisfly richness
•	% coleoptera
•	% gastropoda
•	% diptera
•	Taxa richness
•	% scraper
•	% intolerants
Stream elevation and temperature differed significantly between the reference and test
sites, while stream width did not vary significantly (Table 9), At reference stream sites
there was no significant trend for index scores and elevation (Figure 2).
Table 9. Stream parameters differing for reference verses test sites (* indicates
significance at p<0.05),			
Variable
U (Mann-Whitney)
p-value
Elevation
131.5
0.001*
Temperature
38.5
0.001*
Width
286
0.801
13

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Figure 2. Scatter plot of elevation (m) and index score.
Reference Sites
2
o
o
U)
X

-------
Overall, metrics that comprise the Mil showed a moderate to strong response across
environmental gradients of land-use, water chemistry, and physical habitat. The Mil
itself distinguished between reference and impaired stream sites, and was strongly related
to several potential stressors. The Mil corresponded strongly with the percentage of land
in forest cover. Metrics used to construct the Mil represent multiple categories of
community structure and function and likely provide a good balance of important
ecological information. None of the metrics represented in the index was redundant with
each other and probably gives somewhat different signals of stressors. However,
establishment of measures to quantify biological response signatures to stressors (Yoder
and Rankin 1995), developed for temperate North America, likely will require additional
research on Caribbean streams before their appropriate and successful application.
The index relies on genera level identification of individuals obtained from 100 organism
counts performed in the laboratory. However, identification of chronomid and
oligocheate specimens was done to family level. Specific taxonomic keys for the
macroinvertebrate fauna of Puerto Rico and Caribbean are generally lacking. Throughout
the Caribbean, including Central and South America, there is insufficient taxonomic
knowledge and comprehensive taxakeys (Buss and Vitorino 2010; Jackson and Sweeney
1995). Assignments of pollution tolerance and functional feeding groups for individual
taxa were primarily obtained through the North American literature. Similar to the lack
of taxonomic keys specific to the Caribbean, information on pollution-sensitive taxa and
functional feeding groups is sparse. As this information becomes more available, the
long-term reliability and performance of the index should only improve.
Classification of streams into similar groups is another important consideration in the
development of indexes of biological integrity. Aquatic communities can vary naturally
based on the size, elevation, and geographic position of streams. In a previous benthic
macroinvertebrate study (EPA 1994) of Puerto Rico mountain streams, associations
between biological metrics and water quality and physical habitat were generally weak,
but elevation had a moderately strong association with several of the biological metrics
tested (e.g., trichoptera richness, % gastropoda). However, these findings were
somewhat confounded because the stream sites had reasonable levels of human
disturbance. Nonetheless, elevation became one area of focus for the current study and is
considered at least one important determinant in the structure of freshwater shrimp, a
significant component of the macroinvertebrate assemblage in Puerto Rico streams
(March et al. 2002). To elucidate elevation effects on the macroinvertebrate assemblage,
stream sites in this study were selected from highly forested watersheds and represented a
range of elevations. Selection of highly forested watersheds reduced the likelihood of
more recent human disturbance. The results show the range of elevation studied did not
influence most of the biological measures. Studies of neotropical streams have not
demonstrated clear relationships between elevation and the structure of benthic
macroinvertebrate communities. Pringle and Ramirez (1998) did not find relationships
between altitude and either diversity or taxon richness for streams in Costa Rica.
Harrison and Rankin (1976) noted some zonation of benthic communities along an
15

-------
altitudinal gradient in the major rivers of St, Vincent. However, a number of species
were found at all elevations. In the present study, two of the metrics evaluated, %
triehoptera composition responded positively and % eoleoptera responded negatively to
an increase in elevation, as such, both may require some calibration for elevation if used
in the development of a multimetric index. The proposed Mil had no significant
association with elevation.
Geographic region is another important classification factor to consider in the final
development of any multimetric index. Regional trends for some of the biological
metrics were found in the EPA 2006 study (Kurtenbach 2008). However, due to the
small sample size in that study, it is premature at this time to make any regional
adjustments for the MIL As additional information on the effects of regionalization
become available, the long-term reliability and performance of the index should improve.
Regional changes in the distribution and species composition of aquatic fauna are known
to occur with differences in climate, geology, hydrology, biogeographv, and landscape.
Omemik (1987) used an integration of these factors to develop ecological regions for the
United States. Biological characteristics of streams have been shown to correspond with
ecoregions (Rohm et al. 1987; Whittier et al. 1988; Lyons 1989). In neotropical streams,
regional patterns of community structure and function are not clearly evident (Covich
1988). At present, no ecoregion or similar regional approaches have been developed for
Puerto Rico. Macroinvertebrate data collected from the 2006 survey indicated that
overall taxa richness, including triehoptera and ephemeroptera-trichoptera richness was
lowest in the eastern region. Overall macroinvertebrate abundances and relative
abundances of lepidoptera were also low in this region. Reasons for this observed pattern
are not clear, but could reflect differences in the geology and water chemistry. Lugo
(1986) found that streams in the Luquillo Mountains (eastern region) were usually
oligotrophic. Specific conductance measurements taken in our study were comparably
low. The findings may suggest that the aquatic productivities of these streams are
generally lower and could explain the fewer taxa present and lower abundances of
macroinvertebrates. In contrast, the relative abundances of ephemeroptera and crustacea
were higher in the eastern region, which seems to counter the argument for low stream
productivity. Ephemeropterans were dominated by the family leptoplebiidae, which are
ubiquitous in Puerto Rico across streams ranging from excellent to fair water quality.
The most important component in the development and testing of a multimetric index is
to establish ecological expectations for macroinvertebrate stream communities found in
watersheds that have been relatively free of recent human disturbance. To meet this
condition, stream sites required a minimum of 85% forest land cover upstream of the
sampling point. The intent of this land use requirement was to ensure the streams had
minimum disturbance and most likely represented reference conditions. As defined
(Stoddard et al. 2006), "minimally disturbed condition" implies the absence of significant
human disturbance and the best estimate of a system with biological integrity.

-------
Considering the historical effects of land use change in Puerto Rico (Clark and Wilcock
2000; Birdsey and Weaver 1987; Grau et al. 2003) a less restrictive definition of
reference was used and defined as the "least disturbed condition". This condition is
found in conjunction with the best available physical, chemical, and biological habitat
given today's state of the landscape. Analysis of data collected in a previous study (EPA
1994) did not show significant correlations between metrics and the amount of forest
cover. However, only four of the stream sites had upstream catchments with greater than
80% forest cover, while most had less than 50% forest cover. This compressed the range
of a human disturbance gradient and decreased the likelihood of detecting a biological
response with the biological metrics tested. Calibration of the Mil is based on the 25th
percentile of the reference sites distribution. The Wadeable Streams Assessment
(USEPA 2006) and National Lakes Assessment (USEPA 2010) used this reference
distribution as a benchmark for setting thresholds for biological indicators. Within a
population of reference sites, there are generally two sources of variability. This
variability can be natural or caused by human activities in a watershed, but usually is
some combination of the two. The 25th percentile threshold was set somewhat
conservatively to account for unknown sources of variability. The ultimate selection of
threshold values that are protective of aquatic life depend on the extent to which
reference sites have some degree of human activity.
Conclusions
Index 9, which includes taxa richness, caddisflv richness, % coleopteran, % gastropoda,
% dipteral, % scrapers, and % intolerants is recommended for use for samples collected
from riffle habitat during the spring (February-April) index period. Identification of taxa
at the genera level is required to calculate metrics of the Mil. At this time, application of
the Mil is not recommended outside the interior mountain region of Puerto Rico.
Although the greatest percentage of river miles are found here, other important ecological
regions like the karst limestone and coastal plain have important stream resources that
will require modification of the existing protocol or the development of modified or new
biological assessment techniques.
As the population in Puerto Rico continues to grow and place new demands on water
resources, it is increasingly important to develop cost effective bioassessment protocols
that can be used to assess and monitor biological conditions. In contrast to temperate
North America where rapid bioassessment protocols have been effectively used to
measure environmental conditions, Caribbean streams have unique aquatic characteristics
that require some refinement before biological assessment is effectively used. Current
knowledge on the structure and function of benthic macroinvertcbrate assemblages in
Puerto Rico streams is lacking. This is characteristic of neotropical streams in general, as
Covich (1988) concluded we have an incomplete understanding of biotic diversity and
trophic complexity of these systems. Without this understanding, the use of benthic
macroinvertebrates for detecting environmental changes caused by human disturbance
will be difficult. This study shows that attributes of the benthic macroinvertcbrate
17

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community when incorporated into a multimetric index are responsive to general
stressors that result from human disturbance. However, additional testing of the index is
recommended to determine the geographic extent of use and to examine its sensitivity to
specific groups of pollutants and multiple stressor gradients. Regardless of the study
findings, additional information on the structure and function of benthic
macroinvertebrate assemblages in Puerto Rico streams is needed. Future studies can
provide critical information on species taxonomy, habitat use, pollution tolerances, and
functional feeding group assignments.
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22

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Appendix A-l

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Appendix A-1 stream site identifications with associated latitudes and longitudes (in decimal degrees)
2009 Stream Name
Site ID
Lat
Lon
Collection Date
Rio Morovis
PR09GRAD-001
18.35705
-66.42322
3/11/2009
Rio Cayaguas
PR09GRAD-002
18.14683
-65.93788
3/4/2009
Rio Orocovis
PR09GRAD-003
18.20471
-66.36970
3/16/2009
Non-contributing area - Name not assigned
PR09GRAD-004
18.36461
-66.85813
3/13/2009
Rio de Bayamon at mouth
PR09GRAD-005
18.26359
-66.13059
3/5/2009
Rio Grande de Patillas at Lago Patillas Dam
PR09GRAD-006
18.07240
-66.03949
3/12/2009
Rio Guayanes at mouth
PR09GRAD-007
18.08405
-65.90372
3/5/2009
Rio Canas
PR09GRAD-009
18.30694
-66.04589
3/7/2009
Rio de Bayamon at Lago de Cidra Dam
PR09GRAD-010
18.16725
-66.13052
3/6/2009
Rio Humata
PR09GRAD-011
18.31253
-67.11500
3/18/2009
Rio Caonillas at Lago Caonillas Dam
PR09GRAD-012
18.19552
-66.62402
3/14/2009
Rio Fajardo near mouth
PR09GRAD-013
18.28418
-65.73920
3/14/2009
Rio Grande de Manati
PR09GRAD-014
18.29325
-66.42633
3/10/2009
Rio Bianco
PR09GRAD-015
18.22910
-66.83912
3/13/2009
Rio Guayanilla at mouth
PR09GRAD-016
18.05339
-66.81495
3/11/2009
Rio Morovis
PR09GRAD-017
18,34543
-66.40602
3/10/2009
Rio Valenciano
PR09GRAD-018
18.15838
-65.91971
3/4/2009
Rio Grande de Arecibo at Lago Dos Bocas Dam
PR09GRAD-020
18.25550
-66,71927
3/13/2009
Rio de la Plata at Lago La Plata Dam
PR09GRAD-021
18.30581
-66.20266
3/4/2009
Rio Majada
PR09GRAD-022
18.02936
-66.16324
3/5/2009
Rio Guayanes at mouth
PR09GRAD-023
18.08969
-65.94844
3/5/2009
Rio Yunes
PR09GRAD-024
18.28211
-66.55520
3/9/2009
Rio Gurabo
PR09GRAD-025
18.27816
-65.99055
3/6/2009
Rio de la Plata at Represa Comerio Dam
PR09GRAD-026
18.16366
-66.20926
3/6/2009
Rio Cerrillos at Lago Cerrillos Dam
PR09GRAD-028
18.12750
-66.63653
3/17/2009
Rio Sabana at mouth
PR09GRAD-029
18,31211
-65.72791
3/16/2009
Rio Bauta
PR09GRAD-030
18.19252
-66.43094
3/16/2009
Rio Culebrinas at mouth
PR09GRAD-031
18,30208
-66.92000
3/17/2009
Rio Guaba
PR09GRAD-032
18.15438
-66.96656
3/10/2009
Rio Lajas
PR09GRAD-033
18.35996
-66.27187
3/7/2009
Rio Turabo
PR09GRAD-034
18.18539
-66.05840
3/7/2009
Rio Cuyon
PR09GRAD-035
18.07185
-66.32195
3/9/2009
Rio Pellejas at Lago Pellejas Dam
PR09GRAD-036
18.19089
-66.70215
3/17/2009
Rio Guaynabo
PR09GRAD-037
18.36444
-66.13393
3/4/2009
Rio Toro Negro
PR09GRAD-040
18.28575
-66.49627
3/11/2009
Rio Humacao at mouth
PR09GRAD-041
18.12142
-65.82144
3/6/2009
Rio Guayo
PR09GRAD-042
18.09757
-66.54371
3/11/2009
Rio Blanco
PR09GRAD-043
18.23772
-66.84081
3/13/2009
Rio Usabon
PR09GRAD-046
18.13927
-66.33080
3/9/2009

-------
Appendix A-1 (con't)
2009 Stream Name (con't)
Rio Grande de Anasco at mouth
Rio Corozal
Rio Guavate
Rio Candelero at mouth
Rio Grande de Arecibo at Lago Adjuntas Dam
Laguna San Jose, Laguna Torrecilla, Laguna de Pinones D Watersheds
Rio Arroyata
Quebrada de! Oro
Rio Grande de Arecibo at Lago Dos Bocas Dam
Rio Espiritu Santo near mouth
Rio Bauta
2006 Stream Name
Rio Fajardo
Rio Sabana
Rio Grande
Rio Gurabo
Quebrada Grande
Rio la Plata
Grande de Patillas
Rio Cupeyes
Rio Cruces
Rio Loco
Quebrada Grande
Rio Cain
Rio Maricao
Nueve Pasos
Rio Saliente
Rio Prieto
Rio Yahuecas
Rio las Vacas
Rio Cubuy
1994 Stream Name
Rio Morovis
Rio Unibon
Rio Morovis
Rio Indio
Rio Indio
Rio Sonador
Rio Culebrinas
Site ID
Lat
Lon
Collection Date
PR09GRAD-047
18.27132
-66,96462
3/17/2009
PR09GRAD-049
18.31285
-66.33449
3/7/2009
PR09GRAD-050
18.12101
-66.06185
3/12/2009
PR09GRAD-051
18.10486
-65.84036
3/18/2009
PR09GRAD-052
18.16652
-66.71007
3/17/2009
PR09GRAD-053
18.39210
-66.03213
3/16/2009
PR09GRAD-054
18.19224
-66.21280
3/18/2009
PR09GRAD-055
18.21708
-67.13907
3/19/2009
PR09GRAD-056
18.27520
-66.71158
3/17/2009
PRQ9GRAD-057
18.33762
-65.84691
3/20/2009
PR09GRAD-058
18.22522
-66.46218
3/19/2009
Site ID
Lat
Lon
Collection Date
1
18.27105
-65.725436
4/4/2006
2
18.31785
-65.730676
4/4/2006
4
18.29753
-65.84011
4/5/2006
5
18.249
-65.829231
4/5/2006
6
18.09949
-66.10896
4/6/2006
7
18.09661
-66.071125
4/6/2006
8
18.09112
-66.03472
4/6/2006
16
18.11009
-66.986254
4/7/2006
17
18.10481
-66.977164
4/7/2006
18
18.06728
-66.89793
4/7/2006
19
18.07097
-66.89008
4/7/2006
20
18.12591
-66.99128
4/8/2006
24
18.17017
-66.985048
4/8/2006
25
18.14812
-67,025139
4/8/2006
44
18.17556
-66.550728
4/10/2006
46
18.14035
-66.809631
4/11/2006
47
18,15348
-66.789036
4/11/2006
48
18.12609
-66.755095
4/10/2006
50
18.2622
-65.864984
4/5/2006
Site ID
Lat
Lon
Collection Date
RC81
18.3377
-66.4183
3/13/1994
RC82
18.3441
-66.3774
3/13/1994
RC83
18.3509
-66.4003
3/13/1994
RC84
18.3760
-66.3954
3/13/1994
RC85
18.4090
-66.3886
3/13/1994
RCU90
18.3045
-66.9889
3/18/1994
RCU91
18.3305
-66.9823
3/18/1994

-------
Appendix A-1 (con't)
1994 Stream Name (con't)
Rio Culebrinas
Rio Guatemala
Rio Sanador
Rio Culebrinas
Rio Culebrinas
Rio de Bayamon
Rio de Bayamon
Rio de Bayamon
Rio de Bayamon
Rio de Bayamon
Rio Grande
Rio Espirita Santo
Rio Espirita Santo
Rio Guajataca
Rio Guajataca
Rio Guajataca
Rio Guajataca
Rio Cidra
Rio Cidra
Rio Grande de Arecibo
Rio Pellejas
Rio Grande de Arecibo
Rio Saliente
Rio Grande Jayuya
Rio Grande Jayuya
Rio Grande Jayuya
Rio Jauca
Rio Vivi
Rio Grande de Arecibo
Rio Grande de Arecibo
Rio Grande de Arecibo
Rio Grande de Arecibo
Rio Limon
Rio Yunes
Rio Limon
Rio Limana
Rio Guilarte
Rio Blanco
Rio Prieto
Site ID
Lat
Lon
Collection Date
RCU92
18.3349
-66.9976
3/17/1994
RCU93
18.3562
-66.9959
3/17/1994
RCU94
18.3115
-67.0076
3/17/1994
RCU95
18.3525
-67.0642
3/17/1994
RCU96
18.3597
-67.0922
3/17/1994
RDB50
18.2053
-66.1395
3/13/1994
RDB51
18.2422
-66.1439
3/14/1994
RDB52
18.2607
-66.1336
3/11/1994
RDB53
18.3006
-66.1403
3/14/1994
RDB54
18.3319
-66.1392
3/11/1994
RES76
18.3431
-65.8406
3/10/1994
RES77
18.3461
-65.8241
3/9/1994
RES78
18.3579
-65.8135
3/9/1994
RG86
18.3002
-66.8751
3/17/1994
RG87
18.3304
-66.9161
3/17/1994
RG88
18.3985
-66.9235
3/17/1994
RG89
18.4030
-66.9481
3/17/1994
RGDAOO
18.1633
-66.7268
3/16/1994
RGDA01
18.1797
-66.7364
3/16/1994
RGDA02
18.2216
-66.7213
3/16/1994
RGDA03
18.2299
-66.7171
3/16/1994
RGDA04
18.2461
-66.7221
3/16/1994
RGDA05
18.2067
-66.5536
3/15/1994
RGDA06
18.2181
-66.5907
3/15/1994
RGDA07
18.2090
-66,6189
3/15/1994
RGDA08
18.2042
-66.6290
3/15/1994
RGDA09
18.2168
-66.6426
3/15/1994
RGDA10
18.2591
-66.6851
3/16/1994
RGDA11
18.2663
-66.7087
3/15/1994
RGDA13 .
18.3071
-66.6951
3/15/1994
RGDA14
18.3453
-66.6668
3/15/1994
RGDA15
18.3676
-66.6886
3/15/1994
RGDA16
18.2914
-66.6147
3/15/1994
RGDA17
18.3227
-66.5851
3/15/1994
RGDA18
18.3228
-66.6112
3/15/1994
RGDAN97
18.1982
-66.7973
3/16/1994
RGDAN98
18.1982
-66.7963'
3/16/1994
RGDAN99
18,2409
-66.8633
3/18/1994
RGDAN10Q
18.1996
-66.8846
3/18/1994

-------
Appendix A-1 (con't)
1994 Stream Name (con't)
Rio Bonelii
Rio Cuba
Rio Grande de Anasco
Rio Grande de Anasco
Rio Grande de Anasco
Rio Casey
Rio Gurabo
Rio Gurabo
Rio Grande de Loiza
Rio Grande de Loiza
Rio Cayaguas
Rio Valenciano
Rio Grande de Loiza
Rio Grande de Loiza
Rio Valenciano
Rio Gurabo
Rio Canas
Rio Grande de Loiza
Quebrada Grande
Quebrada Grande
Quebrada Maracute
Rio Canovanillas
Rio Canovanas
Rio Canovanas
Rio Turabo
Rio Turabo
Rio Cubuy
Rio Grande de Manati
Rio Cialitos
Rio Cialitos
Rio Grande de Manati
Rio Grande de Manati
Rio Grande de Manati
Rio Grande de Manati
Rio Grande de Manati
Rio Orocovis
Rio Toro Negro
Rio Matrullas
Rio Bauta
Site ID
Lat
Lon
Collection Date
RGDAN101
18.1795
-66.9606
3/18/1994
RG DAN 102
18.1818
-66.9578
3/18/1994
RGDAN104
18.2554
-66.9164
3/17/1994
RG DAN 105
18.2760
-66.9797
3/18/1994
RGDAN106
18.2818
-67.0507
3/18/1994
RGDAN107
18.2530
-67.0796
3/18/1994
RGDL55
18.2280
-65.8579
3/11/1994
RGDL56
18.2224
-65.8611
3/11/1994
RGDL57
18.1179
-65.9883
3/11/1994
RGDL58
18.1275
-65.9845
3/10/1994
RGDL59
18.1508
-65.9568
3/10/1994
RGDL60
18.1748
-65,9051
3/10/1994
RGDL61
18.1748
-65.9619
3/10/1994
RGDL62
18.2038
-65.9884
3/10/1994
RGDL63
18.2265
-65.9209
3/11/1994
RGDL64
18.2386
-65.8973
3/10/1994
RGDL65
18.2952
-66.0571
3/11/1994
RGDL66
18.3315
-66,0156
3/11/1994
RGDL67
18.3378
-65.9885
3/12/1994
RGDL68
18.3387
-65,9887
3/12/1994
RGDL69
18.3596
-65.9547
3/10/1994
RGDL70
18.3045
-65.9104
3/10/1994
RGDL71
18.3166
-65.8887
3/10/1994
RGDL72
18.3612
-65.8870
3/10/1994
RGDL73
18.1556
-66.0397
3/11/1994
RGDL74
18.1674
-66.0433
3/11/1994
RGDL75
18.2671
-65.8750
3/10/1994
RGDM19
18.3588
-66.4789
3/14/1994
RGDM20
18.3427
-66.4705
3/14/1994
RGDM21
18.3352
-66.4749
3/14/1994
RGDM22
18.3379
-66.4638
3/14/1994
RGDM23
18.3061
-66.4375
3/13/1994
RGDM24
18.2976
-66.4096
3/13/1994
RGDM25
18.2791
-66.3352
3/14/1994
RGDM26
18,2607
-66.2981
3/14/1994
RGDM27
18.2368
-66.3823
3/14/1994
RGDM28
18.2867
-66.4903
3/14/1994
RGDM29
18.2280
-66.4886
3/14/1994
RGDM30
18.2339
-66.4545
3/14/1994

-------
Appendix A-1 (con't)
1994 Stream Name (con't)
Rio de la Plata
Rio de la Plata
Quebrada Beatriz
Rio de la Plata
Rio Maton
Rio de la Plata
Rio de la Plata
Rio Usabon
Rio Hondo
Rio Arroyata
Rio Hondo
Rio de la Plata
Rio Arroyata
Quebrada Higuero
Rio de la Plata
Rio Guadiana
Rio Cuesta
Rio de la Plata
Rio Mameyes
Rio Mameyes.
Site ID
Lat
Lon
Collection Date
RLP32
18.1203
-66.1227
3/12/1994
RLP33
18.1326
-66,1291
3/12/1994
RLP34
18.1347
-66.1316
3/12/1994
RLP35
18.1335
-66.1685
3/12/1994
RLP36
18.1396
-66.2110
3/12/1994
RLP37
18.1436
-66.2127
3/12/1994
RLP38
18.1580
-66.2285
3/12/1994
RLP39
18.1595
-66.3092
• 3/14/1994
RLP40
18.2147
-66.2503
3/13/1994
RLP41
18.1947
-66.2059
3/13/1994
RLP42
18.2088
-66.2432
3/13/1994
RLP43
18.2174
-66.2250
3/13/1994
RLP44
18.2390
-66.2086
3/13/1994
RLP45
18.2387
-66.2120
3/13/1994
RLP46
18.2404
-66.2075
3/13/1994
RLP47
18.2998
-66.2248
3/11/1994
RLP48
18.2970
-66.2064
3/11/1994
RLP49
18.3038
-66.2111
3/11/1994
RM79
18.3272
-65.7504
3/9/1994
RM80
18.3427
-65.7551
3/9/1994

-------
Appendix A-2

-------
AnnonHiY A0 ,n situ field measures (PH- dissolved oxygen, specific conductivity, temperature) and water
Append X	Chemistry data for sites sampled during 1994, 2006, and 2009.
Site J D
Date_Col
DO(mg/L) Temp(.C)
PH
COND

PR09001
3/11/2009
6.8
22.5
7.31
665
PR09001R
3/19/2009
5.4
22.2
7.4
676
PR09001D





PR09002
3/4/2009
8.8
22.8
7.41
185
PR09003
3/16/2009
6.4
20.4
7.25
157
PR09004
3/3/2009
5.3
20.9
7.58
634
PR09005
3/5/2009
8.8
21.8
7.49
197
PR09006
3/12/2009
9.4
18.7
6.79
250
PR09007
3/5/2009
7.5
24.1
7.47
128
PR09009
3/7/2009
5.8
22.6
7.41
410
PR09010
3/6/2009
6.7
20.4
6.73
139
PR09011
3/18/2009
8.4
22.7
7.3
245
PR09012
3/14/2009
10.3
20.5
8.22
264
PR09013
3/14/2009
9.8
20.4
6.76
79
PR09014
3/10/2009
9
20.2
7.89
421
PR09015
3/13/2009
9.1
18.8
7.34
189
PR09016
3/11/2009
7.3
20.7
7.92
681
PR09017
3/10/2009
8.4
21.5
8.17
461
PR09018
3/4/2009
78.1
22.3
6.92
167
PR09020
3/13/2009
8.7
22.7
8.2
251
PR09020R
3/17/2009
7.9
22.8
8.01
256
PR09020D





PR09021
3/4/2009
7.6
22.9
7.71
195
PR09022
3/5/2009
6.8
23.3
7.57
712
PR09023
3/5/2009
7.2
21.8
5.33
107
PR09024
3/9/2009
9.9
18.9
7.76
146
PR09025
3/6/2009
5.7
22.8
7.36
321
PR09026
3/6/2009
8.3
19.6
7.68
240
PR09028
3/17/2009
7.2
19.5
7.84
239
PR09029
3/16/2009
9.1
20.9
6.56
55
PR09030
3/16/2009
8.7
17.9
8.1
180
PR09031
3/17/2009
8.1
21.6
8.14
568
PR09032
3/10/2009
8.1
20.4
7.99

PR09033
3/7/2009
8.3
21
7.85
493
PR09034
3/7/2009
7.7
20.3
7.38
234
PR09034R
3/18/2009
10.1
24.2
8.69
267
PR09034D





PR09035
3/9/2009
2.9
23.8
7.31
839
PR09036
3/17/2009
7.9
20.3
7.29
346
PR09037
3/4/2009
5.6
23.7
7.54
436
PR09040
3/11/2009
9.3
21.9
7.72
174
PR09040R
3/19/2009
8.5
24.8
8.65
179
PR09040D





PR09041
3/6/2009
6
23.2
7.52
397
PR09042
3/11/2009
9.3
25.2
8.58
291

-------
Appendix A2 (cont'd)
Site J D
Date.
_Col DO (mg/l)
Temp(.C)
PH
COND (uS/cm)
PR09043

3/13/2009
8.5
20.6
8.18
210
PR09046

3/9/2009
8
17.7
7.28
143
PR09047

3/17/2009
8.5
26.6
8.9
239
PR09049

3/7/2009
9.3
19.4
7.1
248
PR09049R

3/14/2009
9.1
21.2
8.07
283
PR09049D






PR09050

3/12/2009
9.2
17.5
6.68
50
PR09051

3/18/2009
7.9
22.3
8.39
292
PR09052

3/17/2009
8.2
19.2
8.06
325
PR09053

3/16/2009
3.3
25.2
6.99
350
PR09054

3/18/2009
8.7
19.4
8.25
360
PR09055

3/19/2009
7.5
21.9
7.5
306
PR09056

3/17/2009
8.1
21.5
7.81
307
PR09057

3/20/2009
9.7
21.7
7.62
130
PR09058

3/19/2009
9
19.4
6.84
253
Site J D
Date_
Col DO(mg/L)
Temp(.C)
PH
COND


1
4/4/2006
8.1
23
7.3
67.6

2
4/4/2006
7.6
23.8
7.4
75.5

4
4/5/2006
7.8
20.6
7.8
60.6

5
4/5/2006
7.7
21.3
7.9
61.4

6
4/6/2006
7.2
20.7
7.2
166.5

7
4/6/2006
7.9
20.4
6.4
80.4

8
4/6/2006
7.7
21.7
6.2
78.7

16
4/7/2006
7.8
23.5
7.7
269

17
4/7/2006
7.5
24.2
7.8
263.5

18
4/7/2006
7.1
29
8.1
368.1

19
4/7/2006
7.3
23.1
7.9
448.4

20
4/8/2006
8.3
21.4
7.2
173.6

24
4/8/2006
8.2
21.8
7.3
152.8

25
4/8/2006
7.9
22.8
7.6
229.4

44
4/10/2006
8.3
18.8
7
81.6

46
4/11/2006
7.9
19.7
7.3
104.5

47
4/11/2006
8.2
19.2
7.2
139.9

48
4/10/2006
7.7
19.5

142.3

50
4/5/2006
7.8
21.8
7.8
140
Site J D
Date_
.Col DO (mg/l)
Temp(.C)
PH
COND (uS/cm)
RC-81

3/13/1994
8.6
26
7.77
200
RC-82

3/13/1994
8.8
27
8.1
400
RC-83

3/13/1994
8.2
23.5
7.8
250
RC-84

3/13/1994
8.7
25
8.15
5
RC-85

3/13/1994
7.9
24.5
8.37
550
RCU-90

3/18/1994
6.8
25
8.3
370
RCU-91

3/18/1994
7.6
24.5
6.6
280
RCU-92

3/17/1994
8.5
27
7.7
310
RCU-93

3/17/1994
8.5
26.5
8.04
440
Site ID
Date_Col DO (mg/l)
Temp(.C)
PH
COND (uS/cm)

-------
Appendix A2 (cont'd)
RCU-94
3/17/1994
8.1
26
7.7
180
RCU-95
3/17/1994
8.3
26
7.6
300
RCU-96
3/17/1994
8.1
26
7.8
280
RDB-50
3/13/1994
6.5
25.5
7.8
250
RDB-51
3/14/1994
8.3
24
8
265
RDB-52
3/11/1994
6.9
26
8.27
398
RDB-53
3/14/1994
7.8
26
7.3
240
RDB-54
3/11/1994
7.3
29
7.2
328
RES-76
3/10/1994
7.9
27
7.9
170
RES-77
3/9/1994
7.9
24.5
8.2
100
RES-78
3/9/1994
8.4
22
6.3
61
RG-86
Mar-94
7.3
22.5
7.6
242
RG-87
3/17/1994
8.9
24.5
6.9
365
RG-88
3/17/1994
6.9
26.5
7.43
385
RG-89
3/17/1994
6.1
25.5
6.68
320
RGDA-00
3/16/1994
8.9
26
8.8
210
RGDA-01
3/16/1994
7.8
24
7.4
306
RGDA-02
3/16/1994
8.3
27
8.13
300
RGDA-03
3/16/1994
5
31
8.4
270
RGDA-04
3/16/1994
5
31
8.3
270
RGDA-05
3/15/1994
9
22
6.2
100
RGDA-06
3/15/1994
8.4
25
6.3
140
RGDA-07
3/15/1994
7.8
26
6.7
398
RGDA-08
3/15/1994
6.9
25
8.27
280
RGDA-09
3/15/1994
8.3
23.5
8.37
200
RGDA-10
3/16/1994
7.6
25
8.26
300
RGDA-11
3/15/1994
7.3
25
7.9
300
RGDA-13
3/15/1994
7.7
24
7.5
245
RGDA-14
3/15/1994
4.9
25
7.3
205
RGDA-15
3/15/1994
8.1
26.5
8.81
230
RGDA-16
3/15/1994
9
21
7.65
480
RGDA-17
3/15/1994
9.2
24
7
180
RGDA-18
3/15/1994
8.9
25
6.8
180
RGDAN-97
3/16/1994
9.6
24
6.52
220
RGDAN-98
3/16/1994
9.3
23.5
6.46
250
RGDAN-99
3/18/1994
5.8
24
8.3
265
RGDAN-100
3/18/1994
8.5
25.5
7.92
260
RGDAN-101
3/18/1994
8.8
25
6.66
250
RGDAN-102
3/18/1994
8.9
23
6.25
290
RGDAN-104
3/17/1994
8.9
23
7.6
296
RGDAN-105
3/18/1994
7.4
25
7.84
250
RGDAN-106
3/18/1994
8.2
24
7.8
219
RGDAN-107
3/18/1994
8.9
23
8.2
190
RGDL-55
3/11/1994
8.7
26.5
8.6
240
RGDL-56
3/11/1994
8.6
27
8.5
360
RGDL-57
3/11/1994
7.7
23
8.5
150
RGDL-58
3/10/1994
7.7
25
7.3
215
RGDL-59
3/10/1994
7.8
26
7.2
160
RGDL-60
3/10/1994
8.3
23.5
7.7
380
RGDL-61
3/10/1994
8.2
25
7.6
120
RGDL-62
3/10/1994
6.6
28
8.02
255
Site ID
Date Col DO (mg/l)
Temp(.C)
PH

COND (uS/cm)

-------
Appendix A2 (cont'd)
RGDL-63
3/11/1994
5.5
25
7.8
350
RGDL-64
3/10/1994
10.3
28.5
8.5
370
RGDL-65
3/11/1994
6.6
23.5
7.9
250
RGDL-66
3/11/1994
7.5
28
7.5
310
RGDL-67
3/12/1994
7.8
24.5
7.9
440
RGDL-68
3/12/1994
8.1
25
8.1
500
RGDL-69
3/10/1994
7.2
27
8.6
510
RGDL-70
3/10/1994
7
25.5
8.3
510
RGDL-71
3/10/1994
7.9
25
8.6
260
RGDL-72
3/10/1994
7.5
26.5
8.2
310
RGDL-73
3/11/1994
6.9
24.5
8.2
275
RGDL-74
3/11/1994
7.8
26.5
7.9
184
RGDL-75
3/10/1994
7.5
23
8.1
225
RGDM-19
3/14/1994
7.9
26
7.5
216
RGDM-20
3/14/1994
6.9
25.5
8.66
245
RGDM-21
3/14/1994
8.5
23
7.9
234
RGDM-22
3/14/1994
7.8
27
7.3
290
RGDM-23
3/13/1994
8,3
27
8.34
280
RGDM-24
3/13/1994
7
24
7.6
240
RGDM-25
3/14/1994
6.9
25
7.2
285
RGDM-26
3/14/1994
8.6
23.5
6.9
290
RGDM-27
3/14/1994
8.8
25
8.71
280
RGDM-28
3/14/1994
9.6
26
8.01
210
RGDM-29
3/14/1994
8.3
22
7.98
150
RGDM-30
34407




RLP-32
3/12/1994
6.7
25
7.06
500
RLP-33
3/12/1994
8.2
24.5
8.25
250
RLP-34
3/12/1994
7.1
24
8.5
250
RLP-35
3/12/1994
8.5
26.5
8.5
210
RLP-36
3/12/1994
6.9
26
8.1
500
RLP-37
3/12/1994
6.2
26.5
7.5
700
RLP-38
3/12/1994
7.2
28.5
8.3
465
RLP-39
3/14/1994
6.9
23
8.1
325
RLP-40
3/13/1994
8.6
24.5
8.4
330
RLP-41
3/13/1994
8.8
24.5
7.9
550
RLP-42
3/13/1994
8.8
24.5
8.4
320
RLP-43
3/13/1994
8
27
7.95
450
RLP-44
3/13/1994
7.2
25
8.3
500
RLP-45
3/13/1994
9
24
8.4
280
RLP-46
3/13/1994
7.9
26
8,2
363
RLP-47
3/11/1994
7.5
23
7.7
333
RLP-48
3/11/1994
8.7
28.5
7
240
RLP-49
3/11/1994
6.8
25.5
7.2
410
RM-79-A
3/9/1994
9.4
25
7.8
120
RM-80-A
3/9/1994
7.7
24.5
7.65
130

-------
Appendix A2 (cont'd)
Site J D
CA_PPM K_
PPM
MG_PPM NA_PPM ALKALINITY, CL
_PPM
CL
PR09001
120,000
3,300
6,100
14,000
300
24
676.056
PR09001R
120,000
3,200
7,000
17,000
310
26
732.394
PR09001D
120,000
3,700
7,800
25,000
310
26
732.394
PR09002
15,000
700
4,600
16,000
71
12
338.028
PR09003
14,000
880
7,100
8,500
69
9.6
270.423
PR09004
130,000
970
8,000
8,400
360
14
394.366
PR09005
17,000
2,500
9,000
9,400
68
14
394.366
PR09006
4,300
500
1,900
11,000
26
9.6
270.423
PR09007
6,800
2,700
2,500
13,000
35
10
281.69
PR09009
38,000
3,000
13,000
24,000
140
31
873.239
PR09010
4,800
2,300
5,200
15,000
25
14
394.366
PR09011
32,000
1,900
7,600
11,000
120
8.6
242.254
PR09012
30,000
1,500
8,500
14,000
100
13
366.197
PR09013
3,100
670
1,400
6,900
11
11
309.859
PR09014
60,000
1,100
10,000
15,000
190
16
450.704
PR09015
18,000
1,400
4,900
11,000
66
12
338.028
PR09016
72,000
1,400
43,000
21,000
310
32
901.408
PR09017
60,000
3,900
6,600
22,000
170
28
788.732
PR09018
12,000
850
3,500
15,000
58
13
366.197
PR09020
27,000
1,800
7,200
15,000
89
10
281.69
PR09020R
28,000
1,800
7,400
15,000
92
10
281.69
PR09020D
28,000
1,800
7,500
15,000
92
10
281.69
PR09021
17,000
1,500
5,600
14,000
67
14
394.366
PR09022
73,000
1,000
25,000
52,000
330
46
1295.77
PR09023
7,100
1,100
2,200
12,000
42
9.8
276.056
PR09024
13,000
2,000
4,800
7,400
53
8.7
245.07
PR09025
32,000
3,300
13,000
18,000
100
26
732.394
PR09026
20,000
1,500
11,000
16,000
82
19
535.211
PR09028
26,000
1,600
7,500
11,000
100
9.2
259.155
PR09029
2,900
750
1,800
8,000
15
11
309.859
PR09030
14,000
1,700
9,800
8,000
79
9.7
273.239
PR09031
85,000
2,600
16,000
20,000
220
11
309.859
PR09032
1,700
500
25,000
2,600
99
5.3
149.296
PR09033
75,000
3,300
8,600
22,000
200
29
816.901
PR09034
17,000
1,300
9,100
16,000
78
16
450.704
PR09034R
20,000
1,100
10,000
17,000
96
18
507.042
PR09034D
20,000
1,100
10,000
17,000
95
18
507.042
PR09035
100,000
2,200
31,000
44,000
330
58
1633.8
PR09036
34,000
1,600
13,000
14,000
120
19
535.211
PR09037
45,000
2,400
13,000
25,000
170
32
901.408
PR09040
18,000
1,000
5,300
8,400
69
7.3
205.634
PR09040R
19,000
1,200
5,300
8,700
75
7.1
200
PR09040D
19,000
1,300
5,400
8,600
76
7.2
202.817
PR09041
35,000
1,500
12,000
29,000
150
32
901.408
PR09042
45,000
1,000
5,800
12,000
130
10
281.69

-------
Appendix A2 (cont'd)
Site_ID
CA_PPM K_
PPM
MG_PPM NA_PPM ALKALINITY, CL_PPM
CL
PR09043
21,000
1,500
5,100
11,000
67
16
450.704
PR09046
9,900
3,300
3,900
14,000
40
16
450.704
PR09047
29,000
1,600
8,900
10,000
110
7.6
214.085
PR09049
20,000
2,900
14,000
11,000
99
16
450.704
PR09049R
25,000
3,000
15,000
13,000
120
19
535.211
PR09049D
25,000
3,200
15,000
13,000
120
19
535.211
PR09050
3,700
730
1,500
9,600
19
11
309.859
PR09051
13,000
500
5,300
39,000
90
34
957.746
PR09052
39,000
1,500
11,000
11,000
150
10
281.69
PR09053
27,000
3,400
5,400
26,000
96
38
1070.42
PR09054
34,000
1,100
11,000
23,000
140
27
760.563
PR09055
36,000
2,100
13,000
11,000
140
15
422.535
PR09056
32,000
1,300
12,000
16,000
120
18
507.042
PR09057
9,000
530
4,900
9,000
46
11
309.859
PR09058
29,000
1,000
8,400
9,200
110
12
338.028
Site J D
CA_PPM K_
PPM
MG_PPM NA_PPM ALKALINITY,
CL_PPM
CL

1
2
4






5
6
7
8
16
17
18
19
20
24
25
44
46
47
48
50
Site J D	CA_PPM K_PPM MG_PPM NA_PPM ALKALINITY, CL_PPM CL
RC-81
RC-82
RC-83
RC-84
RC-85
RCU-90
RCU-91
RCU-92
RCU-93
Site ID	CA_PPM K_PPM MG_PPM NA_PPM ALKALINITY, CL_PPM CL

-------
Appendix A2 (cont'd)
RCU-94
RCU-95
RCU-96
RDB-50
RDB-51
RDB-52
RDB-53
RDB-54
RES-76
RES-77
RES-78
RG-86
RG-87
RG-88
RG-89
RGDA-00
RGDA-01
RGDA-02
RGDA-03
RGDA-04
RGDA-05
RGDA-06
RGDA-07
RGDA-08
RGDA-09
RGDA-10
RGDA-11
RGDA-13
RGDA-14
RGDA-15
RGDA-16
RGDA-17
RGDA-18
RGDAN-97
RGDAN-98
RGDAN-99
RGDAN-100
RGDAN-101
RGDAN-102
RGDAN-104
RGDAN-105
RGDAN-106
RGDAN-107
RGDL-55
RGDL-56
RGDL-57
RGDL-58
RGDL-59
RGDL-60
RGDL-61
RGDL-62
Site J D	CA_PPM K_PPM MG_PPM NA_PPM ALKALINITY, CL_PPM CL

-------
Appendix A2 (cont'd)
RGDL-63
RGDL-64
RGDL-65
RGDL-66
RGDL-67
RGDL-68
RGDL-69
RGDL-70
RGDL-71
RGDL-72
RGDL-73
RGDL-74
RGDL-75
RGDM-19
RGDM-20
RGDM-21
RGDM-22
RGDM-23
RGDM-24
RGDM-25
RGDM-26
RGDM-27
RGDM-28
RGDM-29
RGDM-30
RLP-32
RLP-33
RLP-34
RLP-35
RLP-36
RLP-37
RLP-38
RLP-39
RLP-40
RLP-41
RLP-42
RLP-43
RLP-44
RLP-45
RLP-46
RLP-47
RLP-48
RLP-49
RM-79-A
RM-80-A

-------
Appendix A2 (cont'd)
Site J D
N03N_PPM NITRATE (mg/l)
NTL_PPM
ORTHOPHOSPHATE [AS P]
PR09001
0.71
0.97
0.059
PR09001R
0.48
0.75
0.042
PR09001D
0.47
0.77
0.033
PR09002
0.24
0.49
0.014
PR09003
0.39
0.38
0.01
PR09004
0.055
0.23
0.021
PR09005
1.3
1.6
0.012
PR09006
0.087
0.12
0.01
PR09007
0.53
0.79
0.035
PR09009
1.4
1.6
.0.2
PR09010
0.79
1.2
0.01
PR09011
0.48
0.56
0.054
PR09012
2.5
2.2
0.04
PR09013
0.043
0.073
0.01
PR09014
0.63
0.68
0.036
PR09015
1.5
1.6
0.03
PR09016
0.85
0.93
0.12
PR09017
2.6
3.5
0.045
PR09018
0.21
0.26
0.01
PR09020
1.5
1.5
0.081
PR09020R
1.4
1.4
0.062
PR09020D
1.4
1.4
0.063
PR09021
0.8
0.87
0.064
PR09022
0.078
0.19
0.01
PR09023
0.14
0.32
0.01
PR09024
1.1
1.1
0.041
PR09025
1.3
1.5
0.01
PR09026
1.2
1.7
0.01
PR09028
1.1
1.2
0.064
PR09029
0.063
0.09
0.01
PR09030
0.57
0.59
0.081
PR09031
0.45
0.54
0.01
PR09032
0.73
0.74
0.01
PR09033
0.98
1.2
0.01
PR09034
0.26
0.37
0.01
PR09034R
0.077
0.15
0.01
PR09034D
0.076
0.15
0.01
PR09035
1.3
1.3
0.078
PR09036
5.1
4.6
0.052
PR09037
0.65
0.81
0.062
PR09040
0.37
0.41
0.017
PR09040R
0.098
0.17
0.01
PR09040D
0.1
0.16
0.01
PR09041
0.37
0.48
0.043
PR09042
0.35
0.39
0.01

-------
Appendix A2 (cont'd)
Site J D
N03N.
_PPM NITRATE (mg/i)
NTL_
.PPM
ORTHOPHOSPHATE [AS P]
PR09043

2.4

2.1
0.017
PR09046

0.69

0.88
0.055
PR09047

0.25

0.33
0.01
PR09049

1.2

1.3
0.034
PR09049R

1.3

1.4
0.041
PR09049D

1.3

1.4
0.035
PR09050

0.088

0.13
0.01
PR09051

0.13

0.25
0.043
PR09052

1.4

1.4
0.047
PR09053

0.44

2.9
0.031
PR09054

0.068

0.17
0.01
PR09055

0.77

0.99
0.082
PR09056

2.2

1.8
0.066
PR09057

0.17

0.2
0.01
PR09058

0.54

0.52
0.029
Site J D
N03N.
.PPM NITRATE (mg/l)
NTL.
.PPM
ORTHOPHOSPHATE [AS P]
1
2
4
5
6
7
8
16
17
18
19
20
24
25
44
46
47
48
50
Site J D	N03N_PPM NITRATE (mg/l) NTL_PPM ORTHOPHOSPHATE [AS P]
RC-81	0.21
RC-82	0.15
RC-83	0.18
RC-84	0.27
RC-85	0.23
RCU-90	0.18
RCU-91	0.26
RCU-92	0.02
RCU-93	0.22
Site J D	N03N_PPM NITRATE (mg/l) NTL_PPM ORTHOPHOSPHATE [AS P]

-------
Appendix A2 (cont'd)
RCU-94	0.32
RCU-95	0.22
RCU-96	0.28
RDB-50	0.11
RDB-51	0.42
RDB-52	0.22
RDB-53	0.4
RDB-54	0.34
RES-76	0.12
RES-77	0.04
RES-78	0.07
RG-86	1.59
RG-87	0.19
RG-88	0.14
RG-89	0.13
RGDA-00	0.32
RGDA-01	1.18
RGDA-02	0.16
RGDA-03	0.22
RGDA-04	0.31
RGDA-05	0.22
RGDA-06	0.31
RGDA-07	0.31
RGDA-08	0.19
RGDA-09	0.24
RGDA-10	0.13
RGDA-11	0.27
RGDA-13	1.15
RGDA-14	0.4
RGDA-15	0.26
RGDA-16	0.23
RGDA-17	0.14
RGDA-18	0.31
RGDAN-97	0.22
RGDAN-98	0.3
RGDAN-99	0.13
RGDAN-100	0.28
RGDAN-101	0.18
RGDAN-102	0.24
RGDAN-104	0.94
RGDAN-105
RGDAN-106	1.28
RGDAN-107	0.21
RGDL-55	0.03
RGDL-56	0.22
RGDL-57	0.16
RGDL-58	0.18
RGDL-59	0.47
RGDL-60	0.32
RGDL-61	0.2
RGDL-62	0.31
SiteJD	N03N_PPM NITRATE (mg/l) NTL_PPM ORTHOPHOSPHATE [AS P]

-------
Appendix A2 (cont'd)
RGDL-63
0.42
RGDL-64
0.32
RGDL-65
0.77
RGDL-66
0.35
RGDL-67
0.43
RGDL-68
0.44
RGDL-69
0.7
RGDL-70
0.46
RGDL-71
0.16
RGDL-72
0.13
RGDL-73
0.23
RGDL-74
0.17
RGDL-75
0.38
RGDM-19
0.93
RGDM-20
0.14
RGDM-21
0.56
RGDM-22
0.35
RGDM-23
0.15
RGDM-24
1.17
RGDM-25
0.34
RGDM-26
0.19
RGDM-27
0.19
RGDM-28
0.11
RGDM-29
0.28
RGDM-30

RLP-32
0.2
RLP-33
0.03
RLP-34
0.04
RLP-35
0.08
RLP-36
0.17
RLP-37
0.42
RLP-38
2.97
RLP-39
0.16
RLP-40
0.16
RLP-41
0.23
RLP-42
0.5
RLP-43
3.32
RLP-44
0.16
RLP-45
0.64
RLP-46
1.79
RLP-47
1.28
RLP-48
0.16
RLP-49
0.14
RM-79-A
0.07
RM-80-A
0.05

-------
Appendix A2 (cont'd)
SiteJD
PTL_PPM S04_PPM

TOC
DOC
TSS (mg/l)
TURB
PR09001
0.1
23
2.6
2.5
8.8
7.5
PR09001R
0.11
21
3.2
2.9
9.4
12
PR09001D
0.11
21
3.3
3
8.8
12
PR09002
0.23
3.3
1.7
1.1
57
2.9
PR09003
0.039
1.9
0.75
0.8
11
19
PR09004
0.015
3.1
3.5
3
1
0.95
PR09005
0.19
6.6
3.7
3.2
68
54
PR09006
0.022
2.2
0.5
0.75
7.1
4.7
PR09007
0.15
3.9
2.5
1.9
110
42
PR09009
0.26
22
2.7
2.9
2.8
5
PR09010
0.068
17
5.3
5.2
20
35
PR09011
0.071
2.2
0.69
0.63
3.8
5.5
PR09012
0.061
14
1
0.77
1
2.8
PR09013
0.01
3
0.96
1.1
1
1.3
PR09014
0.094
11
0.68
0.85
26
1.7
PR09015
0.036
5.5
0.69
0.7
4.7
6.1
PR09016
0.16
53
1.8
2
1
0.5
PR09017
0.086
16
2
1.9
3.4
1.9
PR09018
0.036
4.8
1.1
0.94
20
2.2
PR09020
0.13
25
0.94
0.77
1.5
2.5
PR09020R
0.11
26
0.84
0.73
1.9
2.3
PR09020D
0.12
26
0.79
0.59
1.6
2
PR09021
0.17
7.3
1.2
0.86
20
13
PR09022
0.029
13
1.3
1
7.3
2.3
PR09023
0.028
1.2
2.3
1.9
6.4
17
PR09024
0.049
3.2
0.5
0.5
1.9
2.6
PR09025
0.033
14
4
3.1
110
120
PR09026
0.072
12
7.6
7.1
5.6
38
PR09028
0.14
4.2
0.5
1.1
13
3.9
PR09029
0.017
2
0.88
1
1
3.5
PR09030
0.1
4
1.1
0.57
2.8
0.85
PR09031
0.017
95
1.3
1.4
13
3.5
PR09032
0.01
3.2
0.75
1.5
1
0.22
PR09033
0.044
27
2.8
2.7
13
13
PR09034
0.029
14
1.9
2.1
6.4
12
PR09034R
0.016
14
1.9
1.5
3
3.9
PR09034D
0.019
13
1.8
1.7
5.8
3.7
PR09035
0.12
82
0.83
0.53
1
0.28
PR09036
0.088
8.4
0.5
0.61
9.4
2.7
PR09037
0.081
11
2.6
2.6
26
4.3
PR09040
0.065
6.2
0.7
0.73
1.9
0.91
PR09040R
0.019
5.8
1.1
0.72
1
7.6
PR09040D
0.031
5.9
0.92
0.82
1.5
0.84
PR09041
0.073
11
1.9
1.8
1.1
0.54
PR09042
0.025
17
0.81
0.9
1
0.54

-------
Appendix A2 (cont'd)
Site J D
PTL (mg/l) S04_PPM

TOC
DOC
TSS (mg/l)
TURB (NTI
PR09043
0.049
5.2
0.83
0.82
25
25
PR09046
0.15
8.4
2.8
2.6
17
48
PR09047
0.02
15
0.9
0.82
2.3
1.5
PR09049
0.094
9.9
1.9
1.4
31
33
PR09049R
0.13
9.7
1.7
1.1
39
36
PR09049D
0.12
9.7
1.7
1.2
39
34
PR09050
0.024
1.7
0.91
0.75
12
5.3
PR09051
0.28
6.7
2.1
1.9
6.2
4.4
PR09052
0.064
4.7
0.51
0.5
6.6
1.8
PR09053
0.28
12
7.3
5.6
10
13
PR09054
0.012
6
2.1
2.1
3.5
0.94
PR09055
0.14
9.2
2.5
2.6
4
12
PR09056
0.1
16
0.97
0.95
3.9
2.4
PR09057
0.011
1.8
2
0.92
1.3
4.1
PR09058
0.058
7.6
0.68
0.56
4
1.8
Site J D	PTL	S04_PPM TOC DOC TSS (mg/l) TURB
1
2
4
5
6
7
8
16
17
18
19
20
24
25
44
46
47
48
50
Site J D
PTL (mg/l)
CO
O
PPM
TOC
DOC
TSS (mg/l)
TURB (NT!
RC-81

0.17



292
5
RC-82

0.11



220
3.3
RC-83

0.15



294
20
RC-84

0.12



282
2.5
RC-85

0.12



310
3
RCU-90

0.16



201
9.8
RCU-91

0.13



150
9.7
RCU-92

0.14



12
11
RCU-93

0.12



267
10
Site J D
PTL (mg/l)
S04
PPM
TOC
DOC
TSS (mg/l)
TURB (NT!

-------
Appendix A2 (cont'd)
RCU-94
0.22


138
22
RCU-95
0.13


196
12
RCU-96
0.11


210
15
RDB-50
0.12


362
10.5
RDB-51
0.05


163
9.2
RDB-52
0.23


172
5.5
RDB-53
0.35


142
27
RDB-54
0.325


224
8.5
RES-76
0.104


126
2.5
RES-77
0.117


73
2.4
RES-78
0.04


38
4.2
RG-86
0.07


152
7.3
RG-87
0.12


225

RG-88
0.12


248

RG-89
0.11


220

RGDA-00
0.25


172
1.5
RGDA-01
0.1


192
0.8
RGDA-02
0.174


182
2.8
RGDA-03
0.19


192
1.5
RGDA-04
0.21


186
1.5
RGDA-05
0.112


134
3
RGDA-06
0.12


90
3.2
RGDA-07
0.12


100
3.1
RGDA-08
0.11


154
5.5
RGDA-09
0.12


122
5
RGDA-10
0.17


240
25
RGDA-11
0.26


212
25
RGDA-13
0.58


223
490
RGDA-14
0.04


132
2.5
RGDA-15
0.26


146
4.4
RGDA-16
0.138


126
1
RGDA-17
0.14


140
1.5
RGDA-18
0.14


142
2.5
RGDAN-97
0.14


136
3.5
RGDAN-98
0.12


174
4
RGDAN-99
0.12


218
7.5
RGDAN-100
0.22


191
15.3
RGDAN-101
0.12


142

RGDAN-102
0.11


184

RGDAN-104
0.05


192
2.2
RGDAN-105
0.126


196
18
RGDAN-106
0.88


141
40
RGDAN-107
0.19


159
7
RGDL-55
0.112


154
2.5
RGDL-56
0.119


221
6.5
RGDL-57
0.2


142
5
RGDL-58
0.23


146
5.5
RGDL-59
0.167


134
7
RGDL-60
0.19


188
3
RGDL-61
0.25


204
20
RGDL-62
0.3


154
29
Site ID
PTL (mg/l) S04_PPM
TOC
DOC
TSS (mg/l)
TURB (NTI

-------
Appendix A2 (cont'd)
RGDL-63
0.25
214
5
RGDL-64
0.165
193
12.4
RGDL-65
0.424
184
5.7
RGDL-66
0.277
214
0
RGDL-67
0.243
313
25
RGDL-68
0.255
336
15
RGDL-69
0.327
351
8.7
RGDL-70
0.211
363
3
RGDL-71
0.124
172
6.5
RGDL-72
0.136
207
3.5
RGDL-73
0.133
122
2.3
RGDL-74
0.108
119
2.5
RGDL-75
0.105
169
8
RGDM-19
0.11
130
61
RGDM-20
0.11
152
5
RGDM-21
0.08
153
1.9
RGDM-22
0.32
176
8.8
RGDM-23
0.24
184
8
RGDM-24
0.14
150
77
RGDM-25
0.22
170
6.5
RGDM-26
0.25
204
4.5
RGDM-27
0.15
161
8
RGDM-28
0.1
130
3
RGDM-29
0.13
100
3.7
RGDM-30



RLP-32
0.111
151
2.5
RLP-33
0.134
150
5
RLP-34
0.117
184
5,5
RLP-35
0.113
220
3.5
RLP-36
0.164
288
1
RLP-37
0.463
338
5
RLP-38
0.53
281
1.9
RLP-39
0.12
216
5
RLP-40
0.11
222
3
RLP-41
0.26
380
3
RLP-42
0.12
243
2.5
RLP-43
0.849
294
6.5
RLP-44
0.127
270
4
RLP-45
0.429
193
7.5
RLP-46
0.16
217
18
RLP-47
0.21
210
0.6
RLP-48
0.122
142
16
RLP-49
0.64
272
10.1
RM-79-A
0.115
91
1.2
RM-80-A
0.122
92
1

-------
Appendix A2 (cont'd)
Site J D
SI02
NITRITE [AS N] AMMONIA [AS N]
PR09001
11,000
0.01
0.016
PR09001R
13,000
0.01
0.017
PR09001D
14,000
0.01
0.017
PR09002
42,000
0.01
0.01
PR09003
38,000
0.01
0.016
PR09004
9,000
0.01
0.01
PR09005
24,000
0.01
0.01
PR09006
29,000
0.01
0.01
PR09007
35,000
0.01
0.033
PR09009
29,000
0.01
0.01
PR09010
28,000
0.014
0.019
PR09011
50,000
0.01
0.01
PR09012
30,000
0.01
0.01
PR09013
16,000
0.01
0.02
PR09014
29,000
0.01
0.01
PR09015
28,000
0.01
0.012
PR09016
38,000
0.01
0.01
PR09017
24,000
0.41
0.77
PR09018
38,000
0.01
0.01
PR09020
40,000
0.01
0.014
PR09020R
41,000
0.01
0.012
PR09020D
40,000
0.01
0.014
PR09021
23,000
0.01
0.026
PR09022
25,000
0.01
0.01
PR09023
37,000
0.01
0.13
PR09024
27,000
0.01
0.01
PR09025
53,000
0.01
0.023
PR09026
32,000
0.01
0.01
PR09028
45,000
0.01
0.01
PR09029
21,000
0.01
0.01
PR09030
30,000
0.01
0.01
PR09031
18,000
0.01
0.01
PR09032
25,000
0.01
0.01
PR09033
26,000
0.01
0.022
PR09034
28,000
0.01
0.041
PR09034R
31,000
0.01
0.01
PR09034D
31,000
0.01
0.01
PR09035
30,000
0.01
0.01
PR09036
32,000
0.01
0.01
PR09037
28,000
0.01
0.026
PR09040
23,000
0.01
0.01
PR09040R
22,000
0.01
0.01
PR09040D
22,000
0.01
0.01
PR09041
34,000
0.01
0.021
PR09042
17,000
0.01
0.01

-------
Appendix A2 (cont'd)
SiteJD
SI02
NITRITE [AS N] AMMONIA [AS N]
PR09043
27,000
0.01
0.013
PR09046
32,000
0.01
0.024
PR09047
25,000
0.01
0.01
PR09049
29,000
0.01
0.022
PR09049R
32,000
0.01
0.031
PR09049D
33,000
0.01
0.027
PR09050
29,000
0.01
0.01
PR09051
46,000
0.01
0.01
PR09052
31,000
0.01
0.01
PR09053
17,000
0.046
2
PR09054
25,000
0.01
0.01
PR09055
33,000
0.01
0.026
PR09056
33,000
0.01
0.01
PR09057
25,000
0.01
0.01
PR09058
24,000
0.01
0.01
SiteJD
SI02
NITRITE [AS N] AMMONIA [AS N]
1
2
4
5
6
7
8
16
17
18
19
20
24
25
44
46
47
48
50
SI02 NITRITE [AS N] AMMONIA [AS N]
SI02 NITRITE [AS N] AMMONIA [AS N]
SiteJD
RC-81
RC-82
RC-83
RC-84
RC-85
RCU-90
RCU-91
RCU-92
RCU-93
Site ID

-------
Appendix A2 (cont'd)
RCU-94
RCU-95
RCU-96
RDB-50
RDB-51
RDB-52
RDB-53
RDB-54
RES-76
RES-77
RES-78
RG-86
RG-87
RG-88
RG-89
RGDA-00
RGDA-01
RGDA-02
RGDA-03
RGDA-04
RGDA-05
RGDA-06
RGDA-07
RGDA-08
RGDA-09
RGDA-10
RGDA-11
RGDA-13
RGDA-14
RGDA-15
RGDA-16
RGDA-17
RGDA-18
RGDAN-97
RGDAN-98
RGDAN-99
RGDAN-100
RGDAN-101
RGDAN-102
RGDAN-104
RGDAN-105
RGDAN-106
RGDAN-107
RGDL-55
RGDL-56
RGDL-57
RGDL-58
RGDL-59
RGDL-60
RGDL-61
RGDL-62
Site J D	SI02 NITRITE [AS N] AMMONIA [AS N]

-------
Appendix A2 (cont'd)
RGDL-63
RGDL-64
RGDL-65
RGDL-66
RGDL-67
RGDL-68
RGDL-69
RGDL-70
RGDL-71
RGDL-72
RGDL-73
RGDL-74
RGDL-75
RGDM-19
RGDM-20
RGDM-21
RGDM-22
RGDM-23
RGDM-24
RGDM-25
RGDM-26
RGDM-27
RGDM-28
RGDM-29
RGDM-30
RLP-32
RLP-33
RLP-34
RLP-35
RLP-36
RLP-37
RLP-38
RLP-39
RLP-40
RLP-41
RLP-42
RLP-43
RLP-44
RLP-45
RLP-46
RLP-47
RLP-48
RLP-49
RM-79-A
RM-80-A

-------
Appendix A-3

-------
Appendix A3 '3hys'cal hab'tat data for sites sampled during 2009 (data not collected in 1994 and 2006).
Site J D
Date_Col
PCT_SA
PCT_FN
XEMBED
XCEMBED
XCL
xcs
PR09001
3/11/2009
32
0
42
49.98
14.75
23
PR09001R
3/19/2009
28
0
47.6
45.98
23.75
20.25
PR09002
3/4/2009
76,4
0
79.27
86.05
18.06
64
PR09003
3/16/2009
0
36
67.8
58.99
3
17.75
PR09004
3/3/2009
8
66
83.6
77.32
5
39.25
PR09005
3/5/2009
20
0
40
35.98
19.75
13
PR09006
3/12/2009
20
0
38
29.98
27
41.25
PR09007
3/5/2009
40
0
57
51.65
0
20.62
PR09009
3/7/2009
55
0
71
69.15
16.25
40
PR09010
3/6/2009
8
28
49.2
36.32
17.75
29.25
PR09011
3/18/2009
20
0
36
37.98
5
29.5
PR09012
3/14/2009
48
4
72.4
71.32
5
4.5
PR09013
3/14/2009
4
0
44.4
45.31
2.5
56.75
PR09014
3/10/2009
4
0
6
2.667
3
57
PR09015
3/13/2009
52
0
67.2
56.002
2.5
22.5
PR09016
3/11/2009
28
4
52.4
63.31
18.5
17.75
PR09017
3/10/2009
8
0
23.6
31.3
21.25
34
PR09018
3/4/2009
80
0
89.27
86.6
19.09
45.4
PR09020
3/13/2009
44
0
54
56.654
5
29.5
PR09020R
3/17/2009
44
4
53.2
61.96
1.5
29.25
PR09021
3/4/2009
31.1
2.8
61.14
60.9
13.2
14.46
PR09022
3/5/2009
20
12
57
45.92
10.5
19
PR09023
3/5/2009
88
12
100
100
3
27.75
PR09024
3/9/2009
0
0
20.6
26.6
11.25
47.25
PR09025
3/6/2009
20
0
40
25.97
39.5
49.75
PR09026
3/6/2009
0
4
39.4
38.62
20.25
23.5
PR09028
3/17/2009
20
0
48.4
53.98
0.5
39.25
PR09029
3/16/2009
0
0
32.8
33.96
19.25
47.5
PR09030
3/16/2009
4
4
26
27.3
8
34
PR09031
3/17/2009
32
4
51.6
53.314
7
34
PR09032
3/10/2009
4
0
24.4
15.32
5
42.5
PR09033
3/7/2009
36
8
48.6
49.3
8
26.75
PR09034
3/7/2009
36
0
57.2
33.3
6.25
20.5
PR09034R
3/18/2009
20
0
61.6
54.64
5.75
5.5
PR09035
3/9/2009
52
0
62,6
59.32
5
8.5
PR09036
3/17/2009
44
0
60
53.98
19.75
44.25
PR09037
3/4/2009
21.8
47.2
78.45
75.4
7.727
25.45
PR09040
3/11/2009
8
0
40.4
38.62
5.5
22.75
PR09040R
3/19/2009
12
0
40.4
32.632
3.5
10.75
PR09041
3/6/2009
72
0
85.6
77.32
7.5
17.25
PR09042
3/11/2009
12
8
39
32.9
8.25
16

-------
Appendix A3
(cont'd)
Site J D
Date_Col
PCT_SA
PCT_FN
XEMBED
XCEMBED
XCL
xcs
PR09043
3/13/2009
36
0
38.8
38
22.5
16
PR09046
3/9/2009
4
4
33.8
34.98
14.5
15
PR09047
3/17/2009
24
8
61.4
52.974
0.5
3.5
PR09049
3/7/2009
20
4
37
35.3
12.75
18.25
PR09049R
3/14/2009
28
12
52.2
45.634
9.25
24.25
PR09050
3/12/2009
60
0
78.8
68
3
75.25
PR09051
3/18/2009
4
0
76.8
78.66
13.75
34.5
PR09052
3/17/2009
28
0
51.6
54.64
19.25
32.25
PR09053
3/16/2009
40
0
64
58.64
26.5
27.5
PR09054
3/18/2009
8
0
25.6
37.32
8
45.75
PR09055
3/19/2009
48
16
78.8
69.98
3
30.75
PR09056
3/17/2009
52
8
78
82.64
0.5
42.5
PR09057
3/20/2009
0
0
26.8
29.96
24.75
19
PR09058
3/19/2009
0
0
12.67
10
0
23.75

-------
Appendix A3
(cont'd)
SiteJD
XMW
XMH
XGW
XGH
XGB XC
XM
XG

PR09001
53.5
1
7
20.5
67
37.75
54.5
27.5
PR09001R
44.5
2.5
12.5
31.75
48.25
44
47
44.25
PR09002
62.5
0
14.65
19.3
67
82.06
62.5
33.95
PR09003
8
5.5
2.5
56.75
9.25
20.75
13.5
59.25
PR09004
28.25
2
5
36
5
44.25
30.25
41
PR09005
43.75
4
14.5
30
12.25
32.75
47.75
44.5
PR09006
82.5
0.83
11.66
10.83
72.08
68.25
83.33
22.49
PR09007
13.75
38.75
3.75
80
18.12
20.62
52.5
83.75
PR09009
34
9
20.25
36
31.75
56.25
43
56.25
PR09010
10.5
10.75
4.5
30.75
30.5
47
21.25
35.25
PR09011
33.25
9
11
16.5
4
34.5
42.25
27.5
PR09012
3.5
4
1
23.5
30
9.5
7.5
24.5
PR09013
76.25
3
17
47.25
25
59.25
79.25
64.25
PR09014
75.25
1
19.5
16.25
59
60
76.25
35.75
PR09015
46.5
9.5
47.25
14.25
32.75
25
56
61.5
PR09016
18.5
1.5
4
2.5
63.5
36.25
20
6.5
PR09017
25.5
0.5
8.5
10.5
75.25
55.25
26
19
PR09018
45.56
0.68
24.54
47.95
16.13
64.49
46.24
72.49
PR09020
11
23.5
5
57.5
2
34.5
34.5
62.5
PR09020R
17.75
20.25
5
54.25
3.5
30.75
38
59.25
PR09021
25.89
11.78
26.25
29.28
4.28
27.66
37.67
55.53
PR09022
22.25
7
5
14.25
34
29.5
29.25
19.25
PR09023
35.25
11
4
73.25
15.75
30.75
46.25
77.25
PR09024
49.25
19
13
74.75
34.5
58.5
68.25
87.75
PR09025
69
3.5
19
34.75
27.5
89.25
72.5
53.75
PR09026
19
0.5
5
5
57.25
43.75
19.5
10
PR09028
41.25
0.5
38
8.5
47.5
39.75
41.75
46.5
PR09029
54.25
12
15
38
34.75
66.75
66.25
53
PR09030
53.5
1.5
12.5
15
5.5
42
55
27.5
PR09031
17
8.25
4.5
24.25
11
41
25.25
28.75
PR09032
29.5
11.75
5
54.25
2.5
47.5
41.25
59.25
PR09033
15
2.5
4
44.5
9
34.75
17.5
48.5
PR09034
24.25
28.5
7
84.5
1
26.75
52.75
91.5
PR09034R
24.25
15
5
78.5
8.5
11.25
39.25
83.5
PR09035
8
6
2
47.75
12.75
13.5
14
49.75
PR09036
31.5
3.5
31.5
11
41.25
64
35
42.5
PR09037
37.95
31.25
36.1
55.79
3.4
33.18
69.2
91.89
PR09040
19
13.75
5
72.5
21
28.25
32.75
77.5
PR09040R
10.5
15.75
5
70
20.5
14.25
26.25
75
PR09041
28.25
10.5
10.5
62
24
24.75
38.75
72.5
PR09042
13.75
19
2.5
38.5
18.75
24.25
32.75
41

-------
Appendix A3
(cont'd)
Site J D
XMW
XMH
XGW
XGH
XGB XC
XM
XG

PR09043
46.25
21
34.75
35
30.5
38.5
67.25
69.75
PR09046
22.75
11.75
18.25
22.25
4
29.5
34.5
40.5
PR09047
1.5
54.25
0
54.25
6.5
4
55.75
54.25
PR09049
23.75
11.25
8.75
43.25
3.5
31
35
52
PR09049R
10
7
7
42.5
5.5
33.5
17
49.5
PR09050
41.5
1
5
7
84.5
78.25
42.5
12
PR09051
60.2
4
9
32.5
45.5
48.25
64.2
41.5
PR09052
50.75
2
23.5
22.75
48.25
51.5
52.75
46.25
PR09053
34.75
6
5
28.75
66.5
54
40.75
33.75
PR09054
66.5
4
21
26.25
41.25
53.75
70.5
47.25
PR09055
4
15
5.5
19
21
33.75
19
24.5
PR09056
12
11.75
4.5
17
24.75
43
23.75
21.5
PR09057
48.75
26.75
12
45
30
43.75
75.5
57
PR09058
58.33
3.33
20.41
20.41
56.67
23.75
61.66
40.82

-------
Appendix A3 (cont'd)
SiteJD
XCM
XCMW
XCMG
XCMGW
XCDENMID
XCDENBK
XFC_ALG
PR09001
92.25
91.25
119.75
98.25
90.29
84.71
0
PR09001R
91
88.5
135.25
101
88.24
79.41
0
PR09002
144.56
144.56
178.51
159.21
99.29
99.41
0
PR09003
34.25
28.75
93.5
31.25
80.00
90.00
0
PR09004
74.5
72.5
115.5
77.5
94.41
94.12
0
PR09005
80.5
76.5
125
91
93.82
98.82
0
PR09006
151.58
150.75
174.07
162.41
93.38
100.00
0
PR09007
73.12
34.37
156.87
38.12
28.68
32.35
0
PR09009
99.25
90.25
155.5
110.5
99.12
98.82
0
PR09010
68.25
57.5
103.5
62
94.41
99.41
0
PR09011
76.75
67.75
104.25
78.75
95.29
98.24
0
PR09012
17
13
41.5
14
53.82
70.59
0
PR09013
138.5
135.5
202.75
152.5
54.71
87.65
0
PR09014
136.25
135.25
172
154.75
98.82
98.24
0
PR09015
81
71.5
142.5
118.75
81.18
84.71
0
PR09016
56.25
54.75
62.75
58.75
95.29
94.12
0
PR09017
81.25
80.75
100.25
89.25
92.35
93.53
0
PR09018
110.73
110.05
183.22
134.59
95.59
96.24
0
PR09020
69
45.5
131.5
50.5
77.94
89.41
0
PR09020R
68.75
48.5
128
53.5
74.71
81.76
0
PR09021
65.33
53.55
120.86
79.8
98.82
99.12
0.83
PR09022
58.75
51.75
78
56.75
82.94
93.53
21.5
PR09023
77
66
154.25
70
67.06
73.53
0
PR09024
126.75
107.75
214.5
120.75
73.53
95.88
0
PR09025
161.75
158.25
215.5
177.25
98.24
98.82
0
PR09026
63.25
62.75
73.25
67.75
99.12
98.82
0
PR09028
81.5
81
128
119
96.18
95.29
0
PR09029
133
121
186
136
87.06
96.47
0
PR09030
97
95.5
124.5
108
78.53
98.24
0
PR09031
66.25
58
95
62.5
95.88
99.41
0
PR09032
88.75
77
148
82
70.29
92.35
0
PR09033
52.25
49.75
100.75
53.75
97.65
98.82
0
PR09034
79.5
51
171
58
32.35
54.12
0
PR09034R
50.5
35.5
134
40.5
25.59
52.94
13
PR09035
27.5
21.5
77.25
23.5
42.94
66.47
5
PR09036
99
95.5
141.5
127
98.53
100.00
0
PR09037
102.38
71.13
194.27
107.23
95.18
99.41
0.45
PR09040
61
47.25
138.5
52.25
12.35
42.94
0
PR09040R
40.5
24.75
115.5
29.75
3.24
33.53
0
PR09041
63.5
53
136
63.5
57.94
61.76
1
PR09042
57
38
98
40.5
33.53
71.76
0

-------
Appendix A3 (cont'd)
Site J D
XCM
XCMW
XCMG
XCMGW XCDENMID XCDENBK
XFC_ALG
PR09043
105.75
84.75
175.5
119.5
93.82
95.29
0
PR09046
64
52.25
104.5
70.5
90.00
95.88
0
PR09047
59.75
5.5
114
0
3.82
52.35
5
PR09049
66
54.75
118
63.5
83.24
88.82
0
PR09049R
50.5
43.5
100
50.5
85.59
89.41
0
PR09050
120.75
119.75
132.75
124.75
100.00
100.00
0
PR09051
112.45
108.45
153.95
117.45
92.06
93.53
0
PR09052
104.25
102.25
150.5
125.75
92.65
99.41
0
PR09053
94.75
88.75
128.5
93.75
99.12
99.41
8
PR09054
124.25
120.25
171.5
141.25
99.71
100.00
0
PR09055
52.75
37.75
77.25
43.25
93.82
98.24
0
PR09056
66.75
55
88.25
59.5
96.47
97.65
0
PR09057
119.25
92.5
176.25
104.5
35.00
71.76
0
PR09058
85.41
82.08
126.23
102.49
95.59
92.94
0

-------
Appendix A3 (cont'd)
Site J D
XFC AQM XFC LWDXFC BRS XFC_LTR XFC_OHV XFC_UCB XFC_RCK XFC_
.HUM
PR09001
0
3
4
12
2
2
6
0
PR09001R
0
1
9
10
2
7
16.5
0
PR09002
0
0
8.63
0.9
5.2
0
11.59
0
PR09003
0
0
4
4
5
5
5
0
PR09004
0
0
1
17
1
7
0
1
PR09005
0
1
5
5
9
0
51
0
PR09006
0
0
11.6
0
10
0
57.5
0
PR09007
5
2.5
15
2.5
5
0
2.5
2.5
PR09009
1
0
27.5
0
18.5
0
1
23.5
PR09010
0
0
23.5
3
15
2
3
0
PR09011
0
0
5
1
2
3
5
0
PR09012
0
0
1
1
4
2
9
0
PR09013
0
1
4
5
8
0
7
0
PR09014
0
0
2
0
2
0
10
0
PR09015
0
17.5
23.5
4
31.5
0
12
0
PR09016
0
0
9
2
1
1
5
0
PR09017
0
1
1
2
2
0
11
0
PR09018
0
0
23.18
0.9
3.18
2.27
18.4
2.27
PR09020
0
0
1
1
3
2
3
0
PR09020R
0
0
4
1
5
3
2
0
PR09021
0.83
0
4.16
8.3
15
0.83
0.83
0
PR09022
13.5
0
8
0
31.5
0
6
0
PR09023
36
0
4
8
33.1
5
0
0
PR09024
1
0
2
3
4
0
24.5
0
PR09025
2
0
9
8
9
2
0
2
PR09026
0
0
2
1
0
16
51
0
PR09028
1
6
13
3
9
0
3
0
PR09029
0
1
4
8
3
0
50.5
0
PR09030
0
0
1
1
4
2
9
0
PR09031
0
0
3
0
1
2
9
0
PR09032
0
0
1
0
1
4
51
0
PR09033
0
2
4
3
2
5
3
0
PR09034
12
0
7
5
13
0
2
1
PR09034R
0
0
4
0
5
0
3
0
PR09035
0
0
0
1
1
0
0
0
PR09036
0
1
17
0
9
0
5
0
PR09037
0.45
0.45
16.59
7.7
26.13
3.63
0.45
6.36
PR09040
0
0
1
0
5
0
26
0
PR09040R
1
0
2
1
5
0
34
0
PR09041
5
0
9
2
5
0
0
2
PR09042
0
0
1
0
5
1
17
0

-------
Appendix A3 (cont'd)
Site J D
XFC_AQM XFC
_LWD XFC_BRS XFC_LTR XFC_OHVXFC_
JJCB XFC.
_RCK
XFCJHUM
PR09043
0
5
5
0
45.5
0
1
0
PR09046
0
0
5
1
4
3
5
0
PR09047
0
0
0
0
3
1
4
0
PR09049
0
0
0
3
2
8
8
1
PR09049R
0
0
0
0
1
4
4
0
PR09050
0
3
57
0
2
0
30
1
PR09051
0
0
30
12
5
2
12
0
PR09052
0
2
8
0
8
0
18.5
0
PR09053
0
0
5
16
4
0
1
5
PR09054
0
0
19.5
1
1
0
2
0
PR09055
0
0
5
2
5
5
0
0
PR09056
0
0
5
3
2
1
4
0
PR09057
0
0
2
0
5
0
50.5
0
PR09058
0
0
1.6
8.3
8.3
0
10
0

-------
Appendix A3 (cont'd)
Site J D
XFC_ALL
PR09001
29
PR09001R
45.5
PR09002
26.32
PR09003
23
PR09004
27
PR09005
71
PR09006
79.1
PR09007
30
PR09009
70.5
PR09010
46.5
PR09011
16
PR09012
17
PR09013
25
PR09014
14
PR09015
88.5
PR09016
18
PR09017
17
PR09018
50.2
PR09020
10
PR09020R
15
PR09021
29.12
PR09022
45.5
PR09023
50.1
PR09024
33.5
PR09025
30
PR09026
70
PR09028
34
PR09029
66.5
PR09030
17
PR09031
15
PR09032
57
PR09033
19
PR09034
28
PR09034R
12
PR09035
2
PR09036
32
PR09037
61.31
PR09040
32
PR09040R
42
PR09041
18
PR09042
24
BIG XFC_NAT W1_HALL
11
29 H
19.5
45.5 M
16.79
26.32 M
10
23 M
2
26 L
61
71 L
67.5
79.1 L
12.5
27.5 M
43
47 H
18
46.5 L
7
16 L
13
17 M
16
25 L
12
14 L
61
88.5 M
6
18 M
14
17 H
23.85
47.93 M
6
10 L
7
15 L
15.83
29.12 L
37.5
45.5 H
33.1
50.1 H
28.5
33.5 L
11
28 M
51
70 L
18
34 L
54.5
66.5 L
13
17 L
10
15 L
52
57 L
7
19 L
16
27 M
8
12 M
1
2 L
15
32 L
33.39
54.95 M
31
32 M
39
42 M
7
16 H
22
24 L

-------
Appendix A3 (cont'd)
SiteJD
XFC_ALL
PR09043
56.5
PR09046
18
PR09047
8
PR09049
22
PR09049R
9
PR09050
93
PR09051
61
PR09052
36.5
PR09053
31
PR09054
23.5
PR09055
17
PR09056
15
PR09057
57.5
PR09058
28.2
BIG XFC_NAT W1_HALL
51.5
56.5 L
9
18 M
7
8 L
11
21 L
5
9 L
36
92 L
17
61 L
28.5
36.5 M
10
26 H
3
23.5 L
5
17 L
6
15 M
55.5
57.5 L
18.3
28.2 M

-------
Appendix A-4

-------
Appendix A-4: Watershed, Forest Land Cover Data
Site J D
Date_Col
PFOR
PR09001
3/11/2009
21.5945
PR09002
3/4/2009
87.3145
PR09003
3/16/2009
22.5413
PR09004
3/3/2009
10.2646
PRQ9005
3/5/2009
69.9877
PR09006
3/12/2009
95.4888
PR09007
3/5/2009
55.1485
PR09009
3/7/2009
67.9994
PR09010
3/6/2009
38.7535
PR09011
3/18/2009
71.1922
PR09012
3/14/2009
69.9149
PR09013
3/14/2009
100
PR09014
3/10/2009
49.133
PR09015
3/13/2009
82.0543
PR09016
3/11/2009
25.1574
PR09017
3/10/2009
0
PR09018
3/4/2009
49.7896
PR09020
3/13/2009
66.7623
PR09021
3/4/2009
88.0056
PR09022
3/5/2009
69.8893
PR09023
3/5/2009
97.4063
PR09024
3/9/2009
74.8673
PR09025
3/6/2009
55.8685
PR09026
3/6/2009
49.8216
PR09028
3/17/2009
59.5488
PR09029
3/16/2009
100
PR09030
3/16/2009
46.2063
PR09031
3/17/2009
58.8224
PR09032
3/10/2009
100
PR09033
3/7/2009
71.713
PR09034
3/7/2009
51.2701
PR09035
3/9/2009
40.8408
PR09036
3/17/2009
99.0829
PR09037
3/4/2009
0
PR09040
3/11/2009
83.6088
PR09041
3/6/2009
51.7909
PR09042
3/11/2009
76.0342
PR09043
3/13/2009
61.3195
PR09046
3/9/2009
44.8818
PR09047
3/17/2009
80.685
PR09049
3/7/2009
0
PR09050
3/12/2009
0
PR09051
3/18/2009
58.4418
PR09052
3/17/2009
81.5648
PR09053
3/16/2009
3.576
PR09054
3/18/2009
82.4497
PR09055
3/19/2009
45.3006

-------
Appendix A-4 {cont'd)
SiteJD	Date_Co!	PFOR %_Fo_90
PR09056	3/17/2009 68.2396
PR09Q57	3/20/2009 75.7963
PR09058	3/19/2009 51.8522
Site ID	Date Col	PFOR %_Fo 90
1
4/4/2006
99.6595
2
4/4/2006
100
4
4/5/2006
100
5
4/5/2006
100
6
4/6/2006
80.6587
7
4/6/2006
91.1667
8
4/6/2006
99.2906
16
4/7/2006
98,011
17
4/7/2006
98.0387
18
4/7/2006
87.7434
19
4/7/2006
92.2579
20
4/8/2006
100
24
4/8/2006
98.9522
25
4/8/2006
96.9312
44
4/10/2006
99.2086
46
4/11/2006
99.8867
47
4/11/2006
98.6951
48
4/10/2006
95.9774
50
4/5/2006
96.905
SiteJD Date_Col PFOR	%_Fo_90
RC81	3/16/1994	4,82
RC82	3/16/1994	13.32
RC83	3/16/1994	7.66
RC84	3/16/1994	13.23
RC85	3/16/1994	15.69
RCU90	3/15/1994	13.36
RCU91	3/15/1994	23.11
RCU92	3/15/1994	20.88
RCU93	3/15/1994	6.29
RCU94	3/15/1994	10.74
RCU95	3/16/1994	12.04
RCU96	3/15/1994	12.24
RDB50	3/15/1994	13.08
RDB51	3/15/1994	12.41
RDB52	3/15/1994	16.36
RDB53	3/15/1994	23.25
RDB54	3/15/1994	22.82
RES76	3/15/1994	64.29
RES77	3/14/1994	89.21
RES78	3/14/1994	81.23
RG86	3/14/1994	20.17
RG87	3/13/1994	16.29
RG88	3/13/1994	13.79
RG89	3/14/1994	13.79
RGDA00	3/14/1994	44.23
RGDA01	3/14/1994	46,49

-------
Appendix A-4 (cont'd)
SiteJD
Date_Col PFOR
%_Fo_90
RGDA02
3/14/1994
43.41
RGDA03
3/14/1994
39.49
RGDA04
3/14/1994
38.95
RGDA05
3/12/1994
76.05
RGDA06
3/12/1994
59.58
RGDA07
3/12/1994
51.61
RGDA08
3/12/1994
48.37
RGDA09
3/12/1994
41.61
RGDA10
3/12/1994
26.69
RGDA11
3/12/1994
36.47
RGDA13
3/14/1994
31.94
RGDA14
3/13/1994
34.76
RGDA15
3/13/1994
35.34
RGDA16
3/13/1994
18.79
RGDA17
3/13/1994
30.20
RGDA18
3/13/1994
28.00
RGDAN97
3/13/1994
53.64
RGDAN98
3/13/1994
51.80
RGDAN99
3/11/1994
48.91
RGDAN100
3/11/1994
44.50
RGDAN101
3/11/1994
1.28
RGDAN102
3/9/1994
41,71
RGDAN104
3/13/1994
30.93
RGDAN105
3/14/1994
53.45
RGDAN106
3/11/1994
35,06
RG DAN 107
3/14/1994
41.62
RGDL55
3/11/1994
59.80
RGDL56
3/11/1994
27.61
RGDL57
3/11/1994
20.77
RGDL58
3/11/1994
19.19
RGDL59
3/10/1994
11.47
RGDL60
3/10/1994
19.07
RGDL61
3/10/1994
15.65
RGDL62
3/10/1994
14.88
RGDL63
3/10/1994
12.58
RGDL64
3/11/1994
20.97
RGDL65
3/10/1994
33.33
RGDL66
3/11/1994
16.82
RGDL67
3/11/1994
14.16
RGDL68
3/12/1994
15.08
RGDL69
3/12/1994
11.98
RGDL7Q
3/10/1994
21.12
RGDL71
3/10/1994
53.52
RGDL72
3/10/1994
41.54
RGDL73
3/10/1994
31.62
RGDL74
3/11/1994
31,25
RGDL75
3/11/1994
68.35
RGDM19
3/10/1994
23.97
RGDM20
3/11/1994
27.55
RGDM21
3/10/1994
27.60
RGDM22
3/9/1994
23.18
RGDM23
3/14/1994
13.54
RGDM24
3/9/1994
13.43
RGDM25
3/13/1994
11.86

-------
Appendix A-4 (cont'd)
Site J D
Date_Col PFOR
%_Fo_90
RGDM26
3/13/1994
11.32
RGDM27
3/13/1994
12,88
RGDM28
3/13/1994
47.02
RGDM29
3/13/1994
49.72
RGDM30
3/17/1994
23.75
RLP32
3/17/1994
56.73
RLP33
3/17/1994
36.12
RLP34
3/16/1994
6.67
RLP35
3/18/1994
35.57
RLP36
3/17/1994
30.35
RLP37
3/17/1994
30.29
RLP38
3/17/1994
29.70
RLP39
3/17/1994
5.70
RLP40
3/17/1994
35.22
RLP41
3/17/1994
12.90
RLP42
3/18/1994
24.42
RLP43
3/18/1994
25.62
RLP44
3/18/1994
23.35
RLP45
3/17/1994
24.59
RLP46
3/18/1994
23.34
RLP47
3/18/1994
19.71
RLP48
3/18/1994
32.35
RLP49
3/14/1994
23.35
RM79
3/14/1994
86.93
RM80
3/9/1994
81.27

-------
Appendix A-5

-------
Appendix A5
Metric data for the sites sampled during 1994, 2006, and 2009
SiteJD
Date_Col
TOTFAM
TAXARICH
ETRICH
CFLYRICHN
CRUSRICH
PR09001
3/11/2009
7
10
1
0
5
PR09001R
3/19/2009
9
13
3
0
5
PR09001D






PR09002
3/4/2009
13
13
5
4
1
PR09003
3/16/2009
10
10
4
1
1
PR09004
3/3/2009
9
9
2
0
1
PR09005
3/5/2009
14
16
4
3
1
PR09006
3/12/2009
13
14
5
3
3
PR09007
3/5/2009
6
8
0
0
4
PR09009
3/7/2009
9
9
2
0
1
PR09010
3/6/2009
16
16
4
2
0
PR09011
3/18/2009
15
18
9
7
0
PR09012
3/14/2009
17
18
6
4
0
PR09013
3/14/2009
14
18
7
5
4
PR09014
3/10/2009
10
12
2
1
3
PR09015
3/13/2009
14
18
9
7
1
PR09016
3/11/2009
13
13
6
4
0
PR09017
3/10/2009
6
6
1
0
1
PR09018
3/4/2009
11
12
4
2
1
PR09020
3/13/2009
19
21
11
8
0
PR09020R
3/17/2009
19
22
11
7
0
PR09020D






PR09021
3/4/2009
8
8
1
0
0
PR09022
3/5/2009
13
14
4
1
1
PR09023
3/5/2009
13
13
0
0
0
PR09024
3/9/2009
14
16
6
5
1
PR09025
3/6/2009
9
9
4
2
1
PR09026
3/6/2009
6
6
0
0
0
PR09028
3/17/2009
18
21
8
7
0
PR09029
3/16/2009
11
14
5
4
3
PR09030
3/16/2009
13
15
7
3
2
PR09031
3/17/2009
12
15
6
2
3
PR09032
3/10/2009
10
10
5
3
0
PR09033
3/7/2009
8
8
2
0
2
PR09034
3/7/2009
17
19
7
5
0
PR09034R
3/18/2009
15
18
8
6
0
PR09034D






PR09035
3/9/2009
12
13
4
2
0
PR09036
3/17/2009
11
12
5
4
0
PR09037
3/4/2009
10
11
2
0
1
PR09040
3/11/2009
12
14
5
3
1
PR09040R
3/19/2009
6
8
3
3
0
PR09040D






PR09041
3/6/2009
15
19
4
0
3
PR09042
3/11/2009
16
18
5
3
2
PR09043
3/13/2009
9
9
2
1
1
PR09046
3/9/2009
10
11
5
2
1
PR09047
3/17/2009
10
12
5
3
1

-------
Appendix A5 (cont'd)
SiteJD
PR09049
PR09049R
PR09049D
PR09050
PR09051
PR09052
PR09053
PR09054
PR09055
PR09056
PR09057
PR09058
Date_CoI
3/7/2009
3/14/2009
3/12/2009
3/18/2009
3/17/2009
3/16/2009
3/18/2009
3/19/2009
3/17/2009
3/20/2009
3/19/2009
TOTFAM TAXAR1CH ETRICH CFLYRICHN CRUSRICH
10
10
11
11
16
8
18
9
12
18
12
13
12
11
12
17
8
20
14
14
22
13
4
3
2
2
6
0
8
3
5
8
5
1
0
4
0
3
0
4
6
4
3
1
4
1
0
1
6
0
3
0
SiteJD
A
Site ID
Date_Col
04-13-2009
TOTFAM TAXARICH ETRICH CFLYRICHN CRUSRICH
13
15

04-13-2009
13
15
9
7

04-06-2009
4
4
0
0

DateCol
TOTFAM
TAXARICH
ETRICH
CFLYRICHN
1
4/4/2006
13
15
9
7
2
4/4/2006
10
9
4
2
4
4/5/2006
11
11
6
4
5
4/5/2006
11
11
5
3
6
4/6/2006
15
16
9
7
7
4/6/2006
14
16
8
6
8
4/6/2006
14
13
7
5
16
4/7/2006
12
12
6
4
17
4/7/2006
16
16
9
7
18
4/7/2006
11
11
5
3
19
4/7/2006
15
15
6
4
20
4/8/2006
12
12
5
3
24
4/8/2006
14
14
8
6
25
4/8/2006
12
12
5
4
44
4/10/2006
14
16
8
6
46
4/11/2006
13
13
5
3
47
4/11/2006
16
17
10
8
48
4/10/2006
11
10
6
5
50
4/5/2006
14
11
5
3
0
1
0
1
3
0
1
0
0
1
1
1
1
2
1
2
2
0
0
1
0
3
SiteJD
Date_Col
TOTFAM
TAXARICH
ETINDEX
CFLYRICHN
RGDA00
• 3/16/1994
12
13
5
4
RGDA01
3/16/1994
9
12
3
2
RGDA02
3/16/1994
11
12
5
3
RGDA03
3/16/1994
18
19
6
4
RGDA04
3/16/1994
11
12
6
4
RGDA05
3/15/1994
12
16
8
6
RGDA06
3/15/1994
9
9
2
1
RGDA07
3/15/1994
12
12
5
3

-------
Appendix
A5
(cont'd)
SiteJD
Date_Col
TOTFAM
TAXARICH
RGDA08
3/15/1994
15
17
RGDA09
3/15/1994
17
20
RGDA10
3/16/1994
15
16
RGDA11
3/15/1994
18
19
RGDA13
3/15/1994
4
5
RGDA14
3/15/1994
7
7
RGDA15
3/15/1994
5
5
RGDA16
3/15/1994
17
19
RGDA17
3/15/1994
16
16
RGDA18
3/15/1994
13
15
RGDM19
3/14/1994
11
11
RGDM20
3/14/1994
12
13
RGDM21
3/14/1994
8
8
RGDM22
3/13/1994
8
10
RGDM23
3/13/1994
9
9
RGDM24
3/14/1994
9
9
RGDM25
3/14/1994
13
14
RGDM26
3/14/1994
10
10
RGDM27
3/14/1994
10
11
RGDM28
3/14/1994
10
12
RGDM29
3/14/1994
10
10
RGDM30
3/12/1994
13
15
RLP32
3/12/1994
13
13
RLP33
3/12/1994
12
12
RLP34
3/12/1994
12
12
RLP35
3/12/1994
11
12
RLP36
3/12/1994
16
16
RLP37
3/12/1994
13
13
RLP38
3/14/1994
10
10
RLP39
3/13/1994
16
19
RLP40
3/13/1994
13
14
RLP41
3/13/1994
10
10
RLP42
3/13/1994
14
16
RLP43
3/13/1994
8
8
RLP44
3/13/1994
13
13
RLP45
3/13/1994
10
11
RLP46
3/11/1994
14
14
RLP47
3/11/1994
9
9
RLP48
3/11/1994
16
17
RLP49
3/9/1994
8
8
RDB50
3/13/1994
8
8
RDB51
3/14/1994
11
12
RDB52
3/11/1994
14
15
ETINDEX CFLYRICHN CRUSRICH
9
6
9
7
6
3
5
3
1
1
2
1
2
1
10
8
8
6
5
3
4
2
5
2
2
1
4
3
3
2
3
1
6
3
5
4
4
1
5
3
5
4
5
3
6
4
7
5
5
3
6
4
8
5
5
4
5
3
8
6
6
4
5
2
9
7
4
3
6
5
3
2
5
3
5
3
5
3
3
2
3
1
5
3
7
5

-------
Appendix A5 (cont'd)
Site ID Date Col TOTFAM
RDB53
3/14/1994
10
RDB54
3/11/1994
3
RGDL55
3/11/1994
7
RGDL56
3/11/1994
11
RGDL57
3/11/1994
12
RGDL58
3/10/1994
12
RGDL59
3/10/1994
10
RGDL60
3/10/1994
14
RGDL61
3/10/1994
13
RGDL62
3/10/1994
13
RGDL63
3/11/1994
10
RGDL64
3/10/1994
12
RGDL65
3/11/1994
16
RGDL66
3/11/1994
3
RGDL67
3/12/1994
11
RGDL68
3/12/1994
10
RGDL69
3/10/1994
6
RGDL70
3/10/1994
10
RGDL71
3/10/1994
8
RGDL72
3/10/1994
7
RGDL73
3/11/1994
15
RGDL74
3/11/1994
16
RGDL75
3/10/1994
13
RES76
3/11/1994
9
RES77
3/10/1994
10
RES78
3/9/1994
11
RM79
3/14/1994
12
RM80
3/9/1994
11
RC81
3/13/1994
4
RC82
3/13/1994
7
RC83
3/13/1994
2
RC84
3/13/1994
12
RC85
3/13/1994
11
RG86
3/17/1994
10
RG87
3/17/1994
9
RG88
3/17/1994
0
RG89
3/16/1994
10
RCU90
3/18/1994
10
RCU91
3/17/1994
8
RCU92
3/17/1994
8
RCU93
3/17/1994
10
RCU94
3/17/1994
10
RCU95
3/17/1994
5
TAXARICH ETINDEX CFLYRICHN CRUSRICH
10
4
2
3
0
0
7
4
2
11
5
2
12
7
4
12
5
3
10
6
4
14
6
5
15
8
6
13
6
3
10
4
1
12
4
1
17
8
6
3
0
0
11
3
1
11
3
2
6
3
1
12
4
2
8
3
1
7
2
0
17
9
7
16
9
6
13
4
2
9
4
3
10
4
3
11
4
2
13
4
2
12
6
5
4
0
0
9
3
2
2
0
0
13
4
1
13
4
3
11
4
1
10
2
1
0
0
0
10
2
1
12
3
1
9
3
1
8
3
1
10
5
2
12
3
1
5
2
1

-------
Appendix A5 (cont'd)
Site ID Date Col TOTFAM
RCU96
3/17/1994
6
RGDAN97
3/18/1994
13
RGDAN98
3/18/1994
10
RGDAN99
3/18/1994
11
RGDAN100
3/17/1994
15
RGDAN101
3/18/1994
13
RGDAN102
3/18/1994
14
RG DAN 104
3/18/1994
9
RGDAN105
3/14/1994
6
RGDAN106
3/14/1994
6
RGDAN107
3/9/1994
10
TAXARICH ETINDEX CFLYRICHN CRUSRICH
6
2
0
15
6
4
12
5
4
14
8
6
17
7
4
15
6
4
16
6
4
10
6
4
7
3
2
7
1
1
12
4
2

-------
Appendix A5 (cont'd)
SiteJD
PRCNTCFCM
PRCNTET PRCNTLEP
PRCNTMFCM
PRCNTCOL
PR09001
0.00
9.71
0.00
9.71
0.97
PR09001R
27.71
59.03
0.00
31.33
3.61
PR09001D





PR09002
22.88
42.37
0.00
19.49
17.80
PR09003
10.38
55,66
0.00
45.28
0.94
PR09004
0.00
60.42
0.00
60.42
0.00
PR09005
6.60
82.08
0.00
75.47
2.83
PR09006
3.54
45.13
0.00
41.59
8.85
PR09007
0.00
0.00
0.00
0.00
3.05
PR09009
0.00
27.27
0.00
27.27
0.00
PR09010
3.00
32.00
0.00
29,00
1.00
PR09011
11.21
66.38
0.00
55.17
17.24
PR09012
10.81
14.41
2.70
3.60
8.11
PR09013
39.66
53.45
0.86
13.79
21.55
PR09014
0.85
16.95
0.00
16.10
3.39
PR09015
12.61
37,82
1.68
25.21
35.29
PR09016
12.50
78.91
0.00
66.41
0.78
PR09017
0.00
4.24
0.00
4.24
0.00
PR09018
2.68
50,00
0.00
47.32
25.00
PR09020
28.46
61.79
7.32
33.33
8.94
PR09020R
25.60
57.60
8.80
32.00
6.40
PR09020D





PR09021
0.00
0.74
0.00
0.74
0.00
PR09022
2.00
28.00
0.00
26.00
4.00
PR09023
0.00
0,00
2,68
0.00
0.00
PR09024
21.67
25.83
10.83
4.17
29.17
PR09025
8.11
56.76
0.00
48.65
8.11
PR09026
0.00
0.00
0.00
0.00
0.00
PR09028
29.70
42.57
0.00
12.87
11.88
PR09029
9.35
26.17
0.00
16.82
35.51
PR09030
3.51
80.70
0.00
77.19
1.75
PR09031
5.38
63.44
0,00
58.06
12.90
PR09032
3.17
73.02
0.79
69.84
5.56
PR09033
0.00
87.83
0.00
87.83
2.61
PR09034
18.52
36.11
0.00
17.59
18.52
PR09034R
23.53
38.24
0.98
14.71
25.49
PR09034D





PR09035
3.54
53.10
0.00
49.56
3.54
PR09036
22.12
58.41
0.00
36.28
23.89
PR09037
0.00
59.80
0.00
59.80
0.98
PR09040
29.57
32.26
1.61
2.69
16.67
PR09040R
5.56
5.56
0.00
0.00
6.35
PR09040D





PR09041
0.00
13.08
0.00
13.08
7.48
PR09042
5.26
8.77
1.75
3.51
41.23
PR09043
20.00
30,00
0.00
10.00
0.00
PR09046
1.87
81.31
0.00
79.44
3.74
PR09047
39.47
52.63
0.00
13.16
36.84

-------
Appendix A5 (cont'd)




SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
PR09049
1.67
64.17
0.00
62.50
14.17
PR09049R
2.15
75.27
0.00
73.12
5.38
PR09049D





PR09050
26.50
63.25
0.00
36.75
0.85
PR09051
0.00
8.93
0.00
8.93
2.68
PR09052
43.27
58.65
0.00
15.38
4.81
PR09053
0.00
0.00
0.00
0.00
0.00
PR09054
10.91
26.36
0.00
15.45
7.27
PR09055
0.00
10.08
0.00
10.08
0.00
PR09056
29.17
32.50
0.83
3.33
35.00
PR09057
11.57
48.76
2.48
37.19
16.53
PR09058
5.50
40.37
0.00
34.86
22.94
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
A
33.90
36.44
1.69
2.54
12.71
B
12.28
70.17
1.75
57.89
4.39
C
0.00
0.00
0.00
0.00
0.00
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
1
I 26
42
13
17
21
2 4
70
0
66
5
4 5
77
0
72
2
5 4
86
0
82
2
6 28
58
11
30
10
7 25
28'
• 7
3
1
8 9
22
0
13
7
16 7
56
0
49
23
17 13
51
10
38
24
18 8
21
0
13
58
19 14
34
0
20
36
20 3
65
4
62
9
24 32
70
1
38
20
2£
i 5
45
0
40
41
44
¦ 58
66
8
8
1
46
i 44
64
3
20
7
47
33
64
4
31
8
48
I 27
53
1
26
5
50
i 10
70
0
60
11
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
RGDAOO
16
26
13
10
6
RGDA01
3
4
3
1
21
RGDA02
54
60
0
6
9
RGDA03
16
27
2
11
42
RGDA04
51
66
0
15
9
RGDA05
22
35
6
13
11
RGDA06
3
8
0
5
3
RGDA07
34
45
0
11
1

-------
Appendix A5 (cont'd)
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
RGDA08
43
51
3
9
5
RGDA09
24
44
3
20
11
RGDA10
10
53
0
42
29
RGDA11
15
30
1
20
14
RGDA13
46
46
0
0
5
RGDA14
6
14
0
8
0
RGDA15
2
9
0
7
1
RGDA16
27
37
11
12
9
RGDA17
29
55
11
27
1
RGDA18
24
33
3
14
8
RGDM19
26
34
2
7
6
RGDM20
5
20
1
15
7
RGDM21
18
23
1
5
2
RGDM22
61
71
1
10
2
RGDM23
10
11
2
1
0
RGDM24
5
27
0
22
4
RGDM25
27
69
0
41
3
RGDM26
7
11
0
4
0
RGDM27
9
43
0
34
5
RGDM28
9
15
0
6
8
RGDM29
4
69
0
65
15
RGDM30
13
21
1
8
8
RLP32
25
53
0
28
0
RLP33
22
46
0
24
1
RLP34
24
59
0
34
2
RLP35
8
12
8
4
0
RLP36
27
39
2
12
0
RLP37
8
19
4
11
0
RLP38
64
68
0
4
2
RLP39
31
55
1
23
3
RLP40
29
81
1
58
4
RLP41
5
. 71
3
66
0
RLP42
26
66
2
41
8
RLP43
15
16
0
1
2
RLP44
22
46
2
24
2
RLP45
4
11
1
7
1
RLP46
18
30
1
12
3
RLP47
12
19
0
7
0
RLP48
19
61
0
42
6
RLP49
16
19
0
3
1
RDB50
76
80
0
4
0
RDB51
10
39
1
29
15
RDB52
25
57
4
32
5

-------
Appendix A5 (cont'd)
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFGM
PRCNTCOL
RDB53
2
10
0
8
7
RDB54
0
0
0
0
2
RGDL55
60
82
0
22
0
RGDL56
2
29
0
27
0
RGDL57
18
24
1
6
2
RGDL58
26
28
1
2
1
RGDL59
12
17
0
5
2
RGDL60
14
25
1
11
1
RGDL61
55
69
2
14
3
RGDL62
10
20
3
10
2
RGDL63
18
45
0
27
1
RGDL64
16
35
0
20
0
RGDL65
13
55
0
43
4
RGDL66
0
0
0
0
0
RGDL67
5
20
0
15
2
RGDL68
4
11
0
7
3
RGDL69
8
14
0
6
4
RGDL70
9
18
0
10
17
RGDL71
9
31
0
22
3
RGDL72
0
48
0
48
0
RGDL73
37
68
5
33
5
RGDL74
40
71
1
31
2
RGDL75
12
17
0
5
5
RES76
15
23
0
8
10
RES77
5
25
0
20
11
RES78
12
27
0
14
1
RM79
2
13
0
11
3
RM80
7
35
0
28
3
RC81
0
0
0
0
0
RC82
24
48
0
24
9
RC83
0
0
0
0
0
RC84
13
19
1
6
2
RC85
4
6
0
2
9
RG86
8
40
0
37
10
RG87
5
38
0
33
6
RG88
0
0
0
0
0
RG89
4
6
0
2
1
RCU90
7
82
0
76
7
RCU91
1
37
0
35
19
RCU92
2
17
1
15
2
RCU93
4
60
0
56
0
RCU94
4
59
0
54
27
RCU95
1
2
0
1
0

-------
Appendix A5 (cont'd)
SiteJD
PRCNTCFCM
PRCNTET
PRCNTLEP
PRCNTMFCM
PRCNTCOL
RCU96
0
5
0
5
0
RGDAN97
39
53
1
15
8
RGDAN98
18
20
1
2
44
RGDAN99
12
37
0
25
22
RGDAN100
14
65
1
50
10
RGDAN1Q1
11
28
4
17
56
RG DAN 102
8
27
1
19
46
RGDAN104
20
30
0
10
16
RGDAN105
7
9
0
2
13
RGDAN106
3
3
3
0
4
RGDAN107
4
25
0
21
26

-------
Appendix A5 (cont'd)
SiteJD
PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS PRCNTCHIR
PR09001
7.77
2.91
0.00
77.67
7.77
PR09001R
1.20
9.64
0.00
26.50
1.20
PR09001D





PR09002
25.42
0.00
1.69
5.93
21.19
PR09003
39.62
0.00
0.94
1.89
35.85
PR09004
10.42
0.00
27.08
2.08
2.08
PR09005
4.72
2.83
1.89
1.89
2.83
PR09006
28.32
0.00
0.00
16.81
3.54
PR09007
0.76
41.22
0.00
54.20
0.76
PR09009
22.73
0.00
4.55
4.55
9.09
PR09010
15.00
0.00
5.00
0.00
13.00
PR09011
6.90
0.86
0.00
0.00
4.31
PR09012
36.04
0.90
3.60
0.00
28.83
PR09013
6.90
0.00
0.00
15.52
6.03
PR09014
14.41
0.00
0.00
60.17
13.56
PR09015
22.69
0.00
0.00
0.84
15.97
PR09016
9.38
0.00
9.38
0.00
7.03
PR09017
94.07
0.85
0.00
0.85
91.53
PR09018
18.75
0.00
3.57
2.68
8.93
PR09020
15.45
3.25
0.81
0.00
5.69
PR09020R
15.20
5.60
0.80
0.00
2.40
PR09020D





PR09021
22.06
0.00
0.00
0.00
17.65
PR09022
13.00
3.00
0.00
1.00
9.00
PR09023
5.36
4.46
1.79
0.00
3.57
PR09024
6.67
0.00
7.50
16.67
0.00
PR09025
8.11
0.00
0.00
23.42
5.41
PR09026
14.29
2.38
2.38
0.00
9.52
PR09028
42.57
0.00
0.99
0.00
26.73
PR09029
19.63
0.00
0.00
18.69
17.76
PR09030
6.14
0.00
4.39
6.14
1.75
PR09031
15.05
0.00
3.23
5.38
12.90
PR09032
6.35
0.00
14.29
0.00
5.56
PR09033
6.09
0.00
0.00
1.74
3.48
PR09034
10.19
4.63
3.70
0.00
5.56
PR09034R
17.65
0.98
1.96
0.00
17.65
PR09034D





PR09035
3.54
19.47
20.35
0.00
3.54
PR09036
12.39
0.00
3.54
0.00
10.62
PR09037
7.84
16.67
0.98
3.92
6.86
PR09040
8.06
40.32
0.00
0.54
7.53
PR09040R
2.38
85.71
0.00
0.00
2.38
PR09040D





PR09041
35.51
18.69
3.74
8.41
30.84
PR09042
9.65
14.04
7.89
6.14
6.14
PR09043
60.00
0.00
0.00
5.00
35.00
PR09046
10.28
0.00
0.93
0.93
10.28
PR09047
6.14
3.51
0.00
0.88
2.63

-------
Appendix A5 (cont'd)
SiteJD

PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS PRCNTCHIR
PR09049

3.33
0.00
0.00
16.67
3.33
PR09049R
7,53
0.00
1.08
8.60
7.53
PR09049D





PR09050

30.77
0.00
0.00
0.85
19.66
PR09051

42.86
0.00
0.00
45.54
36.61
PR09052

28.85
0.00
2.88
1.92
17.31
PR09053

4.17
50.83
0.00
0.00
3.33
PR09054

48.18
3.64
7.27
1.82
40.00
PR09055

3.36
16.81
0.00
62.18
3.36
PR09056

9.17
0.00
20.83
0.00
6.67
PR09057

17.36
7.44
0.00
5.79
14.88
PR09058

35.78
0.00
0.00
0.00
5.50
SiteJD

PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS
PRCNTCHIR |
A

40.68
5.93
0.00
0.00
17.80
B

21.93
0.00
0.88
0.88
0.00
C

0.00
0.00
0.00
0.00
0.00
SiteJD

PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS
PRCNTCHIR |

1
13
0
5
3
11

2
2
0
0
23
1

4
14
0
6
0
4

5
4
0
2
5
2

6
19
0
0
0
16

7
63
0
2
0
20

8
59
0
4
8
55

16
20
0
0
1
14

17
12
0
1
1
10

18
17
3
0
1
7

19
20
0
1
8
11

20
7
0
4
10
5

24
5
0
1
2
5

25
8
0
2
5
5

44
14
0
10
0
3

46
20
1
3
0
13

47
18
0
5
1
9

48
2
0
40
0
2

50
7
0
0
11
6
SiteJD

PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS
PRCNTCHIR
RGDAOO

30
10
3

30
RGDA01

28
1
1

27
RGDA02

17
1
9

12
RGDA03

4
12
7

1
RGDA04

19
6
0

7
RGDA05

34
0
10

24
RGDA06

47
0
0

23
RGDA07

30
11
4

9

-------
Appendix A5 (cont'd)
SiteJD
PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS PRCNTCHIR
RGDA08
38
3
0
6
RGDA09
31
1
7
28
RGDA10
3
0
7
1
RGDA11
32
3
2
14
RGDA13
0
48
0
0
RGDA14
28
43
0
28
RGDA15
15
75
0
15
RGDA16
14
0
21
11
RGDA17
13
1
15
13
RGDA18
47
4
2
4
RGDM19
11
44
0
11
RGDM20
21
49
0
19
RGDM21
36
38
0
25
RGDM22
12
11
0
11
RGDM23
6
54
0
4
RGDM24
4
63
0
2
RGDM25
15
12
0
13
RGDM26
86
1
1
10
RGDM27
47
0
4
27
RGDM28
1
75
1
1
RGDM29
5
0
0
5
RGDM30
27
24
0
26
RLP32
39
5
1
13
RLP33
48
1
0
22
RLP34
28
5
1
22
RLP35
46
29
2
24
RLP36
30
21
8
20
RLP37
11
60
0
7
RLP38
26
3
0
8
RLP39
27
2
9
23
RLP40
7
0
1
5
RLP41
2
21
3
2
RLP42
19
2
0
5
RLP43
20
60
0
0
RLP44
2
37
1
2
RLP45
60
21
3
56
RLP46
18
46
1
7
RLP47
76
2
2
61
RLP48
16
7
3
13
RLP49
9
69
0
9
RDB50
4
15
0
2
RDB51
41
3
1
30
RDB52
9
23
0
9

-------
Appendix AS (cont'd)
SiteJD
PRCNTDIP
PRCNTGAS
PRCNTODON
PRCNTCRUS PRCNTCHIR
RDB53
10
69
0
9
RDB54
1
97
0
1
RGDL55
13
5
0
2
RGDL56
18
40
0
2
RGDL57
69
0
0
12
RGDL58
63
3
0
14
RGDL59
82
0
0
5
RGDL60
9
57
0
4
RGDL61
5
20
0
4
RGDL62
26
47
0
13
RGDL63
47
6
1
16
RGDL64
22
36
1
15
RGDL65
28
5
7
25
RGDL66
43
35
0
43
RGDL67
5
67
1
4
RGDL68
9
67
0
9
RGDL69
13
68
0
13
RGDL70
37
23
2
34
RGDL71
49
15
0
42
RGDL72
21
24
6
20
RGDL73
9
7
0
4
RGDL74
17
7
1
6
RGDL75
27
45
1
22
RES76
64
4
0
41
RES77
41
20
0
38
RES78
55
14
0
54
RM79
74
5
2
74
RM80
60
0
0
59
RC81
61
0
0
59
RC82
9
32
2
9
RC83
100
0
0
97
RC84
40
35
0
38
RC85
4
76
0
3
RG86
41
0
1
39
RG87
43
0
11
39
RG88
0
0
0
0
RG89
32
55
0
28
RCU90
5
2
1
3
RCU91
2
37
4
1
RCU92
38
23
0
16
RCU93
8
28
3
7
RCU94
8
0
1
4
RCU95
0
96
0
0

-------
Appendix A5 (cont'd)
Site ID PRCNTDIP PRCNTGAS PRCNTODON PRCNTCRUS PRCNTCHIR
RCU96
2
92
0
RGDAN97
19
0
15
RGDAN98
17
0
9
RGDAN99
16
24
0
RGDAN100
11
5
3
RGDAN101
8
0
1
RGDAN102
12
0
11
RGDAN104
1
53
0
RGDAN105
1
77
0
RG DAN 106
5
85
0
RGDAN107
41
0
2
1
18
17
15
9
5
7
0
1
4
38

-------
Appendix AS (cont'd)
SiteJD
PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNT1TOL
PRCNTPRD
PR09001
0.97
81.55
40.77
5.80
5.82
PR09001R
0.00
63.86
27.70
8.43
8.43
PRQ9001D





PR09002
6.78
12.71
21.18
5.90
8.32
PR09003
0.94
2.83
43.39
1.88
2.83
PR09004
0.00
2.08
58.33
0.00
27.08
PR09005
2.83
8.49
75.47
1.88
4.71
PR09006
0.88
17.70
24.77
28.30
15.04
PR09007
0.76
96.18
41.98
0.00
9.16
PR09009
40.91
45.45
31.80
4.54
9.09
PR09010
41.00
47.00
40.00
0.00
10.00
PR09011
0.86
9.48
54.31
4.31
9.48
PR09012
32.43
35.14
32.43
0.90
10.81
PR09013
0.00
17.24
33.62
6.03
2.58
PR09014
5.08
65.25
56.77
0.00
2.54
PR09015
1.68
2.52
24.36
1.68
0.84
PRQ9016
0.00
1.56
65.60
0.00
10.93
PR09017
0.00
1.69
91.50
0.00
2.54
PR09018
0.00
2.68
45.53
4.46
6.25
PR09020
0.00
5.69
24.39
8.94
4.87
PR09020R
4.80
11.20
12.00
4.00
2.40
PR09020D





PR09021
75.74
77,21
72.79
0.00
2.20
PR09022
49.00
55.00
49.00
1.00
3.00
PR09023
12.50
90.18
66.96
0.00
8.03
PR09024
0.00
20.00
16.67
24,20
28.30
PR09025
0,00
27.03
45.04
23.40
27.02
PR09026
80.95
83.33
80.95
0.00
2.38
PR09028
0.00
1.98
26.73
3.96
6.93
PR09029
0.00
18.69
29.90
6.12
3.06
PR09030
0.00
7.02
48.24
9.65
10.52
PR09031
0.00
5.38
47.31
4.30
4.30
PR09032
0.00
0.00
57.14
0.00
14.28
PR09033
1.74
3.48
86.08
0.87
1.73
PR09034
14.81
31.48
16.67
2.78
17.59
PR09034R
6.86
15.69
22.54
4.90
9.80
PR09034D





PR09035
0.00
19.47
41.59
0.00
23.00
PR09036
1 77
1.77
36.28
0.00
4.42
PR09037
0.98
30.39
57.84
3.92
13.72
PR09040
0.00
41.40
40.32
8.60
1.07
PR09040R
0.00
85.71
85.71
3.97
0.00
PR09040D





PR09041
12.15
40.19
30.84
0.00
8.41
PR09042
0.88
30.70
28.07
3.50
23.68
PR09043
5.00
10.00
35.00
10.00
15.00
PR09046
2.80
3.74
76.63
0.93
1.86
PR09047
0.00
4.39
29.82
29.80
0.00

-------
Appendix A5 (cont'd)




SiteJD

PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD
PR09049

1.67
18.33
50.80
5.00
4.16
PR09049R
1.08
10.75
72.04
3.22
5.37
PR09049D





PR09050

4.27
5.13
36.75
0.85
1.70
PR09051

0.00
45.54
36.60
2.67
9.82
PR09052

1.92
4.81
21.15
2.88
5.76
PR09053

25.83
95.83
40.83
0.00
19.17
PR09054

5.45
10.91
40.00
1.81
9.09
PR09055

7.56
86.55
47.05
1.68
3.36
PR09056

0.00
1.67
20.80
0.83
23.30
PR09057

0.83
14.88
36.36
6.60
4.13
PR09058

0.92
0.92
31.19
12.80
0.00
SiteJD

PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD
A

2.00
10.17
22.03
10.17
2.54
B

0.00
0.88
47.37
20.18
1.75
C

100.00
100.00
88.89
0.00
0.00
SiteJD

PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD

1
0
3
21
2
7.07

2
0
23
65
4
4

4
0
0
38
10
6.06

5
0
5
65
7
2.13

6
4
4
27
8
0.98

7
0
0
30
14
2.97

8
0
8
55
13
13.86

16
0
2
38
4
6.93

17
0
1
30
10
2.86

18
0
5
58
1
0.99

19
1
9
36
11
2.63

20
0
10
61
12
15.15

24
0
2
37
4
2.13

25
0
5
41
6
7.7

44
0
0
48
8
11.21

46
3
4
33
0
3.8

47
0
1
27
13
4.85

48
0
0
40
1
39.6

50
1
12
59
11
4.95
SiteJD

PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD
RGDAOO

11
22
30
0.00
4.90
RGDA01

42
43
42
0.00
0.99
RGDA02

4
5
41
2.04
9.18
RGDA03

3
18
42
1.02
12.24
RGDA04

0
6
37
11.00
1.00
RGDA05

0
5
24
4.95
14.85
RGDA06

36
38
36
0.98
2.94
RGDA07

8
20
24
6.41
3.85

-------
Appendix A5 (cont'd)
SiteJD
PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD
RGDA08
0
3
37
0.99
0.00
RGDA09
3
5
28
5.00
11.00
RGDA10
5
7
31
0.99
9.90
RGDA11
13
17
18
6.12
4.08
RGDA13
1
49
48
44.90
0.00
RGDA14
14
47
43
0.00
0.00
RGDA15
0
75
75
0.00
0.00
RGDA16
3
5
21
9.38
28.13
RGDA17
3
5
20
17.82
18.81
RGDA18
0
6
48
12,24
6.12
RGDM19
2
46
44
1.01
0.00
RGDM20
0
50
49
0.00
2.04
RGDM21
0
39
38
0.00
2.00
RGDM22
2
13
61
0.00
0.00
RGDM23
27
81
54
5.05
0.00
RGDM24
2
65
63
0.00
0.00
RGDM25
0
13
26
0.00
2.02
RGDM26
1
2
76
3.00
1.00
RGDM27
0
1
27
1.05
5.26
RGDM28
1
75
74
0.00
2.86
RGDM29
6
10
63
3.06
7.14
RGDM3Q
16
41
26
1.00
1.00
RLP32
1
7
27
8.91
2.97
RLP33
0
4
26
0.97
5.83
RLP34
1
10
31
4.12
5.15
RLP35
0
31
30
0.00
4.04
RLP36
0
21
21
0.99
7.92
RLP37
4
66
61
5.66
1.89
RLP38
0
4
55
5.94
1.98
RLP39
3
5
23
0.00
8.91
RLP40
0
6
50
0.93
11.21
RLP41
0
21
63
0.00
3.03
RLP42
2
5
34
1.01
2.02
RLP43
1
63
62
1.27
0.00
RLP44
1
47
37
0.94
17.92
RLP45
2
23
56
0.00
3.13
RLP46
0
46
46
8.00
1.00
RLP47
0
2
61
9.09
2.02
RLP48
4
14
37
3.85
7.69
RLP49
0
71
70
0.00
2.02
RDB50
1
16
76
0.00
0.00
RDB51
0
3
30
0.00
1.03
RDB52
1
25
28
10.64
3.19

-------
Appendix A5 (cont'd)
SiteJD
PRCNTOLIG
PRCNTNINS
PRCNTDOMI
PRCNTITOL
PRCNTPRD
RDB53
2
73
69
0.00
4.17
RDB54
0
97
97
0.00
0.00
RGDL55
0
5
32
32.00
0.00
RGDL56
8
53
40
0.00
0.00
RGDL57
0
3
58
1.94
7.77
RGDL58
2
6
49
10.10
2.02
RGDL59
0
0
75
0.98
0.98
RGDL60
2
64
58
6.00
8.00
RGDL61
1
21
44
44.55
0.00
RGDL62
2
49
47
4.04
0.00
RGDL63
0
6
30
0.00
1.00
RGDL64
5
42
36
0.00
0.98
RGDL65
0
6
40
6.00
12.00
RGDL66
21
57
43
0.00
0.00
RGDL67
6
72
66
0.00
0.99
RGDL68
7
77
67
0.99
4.95
RGDL69
0
69
69
0.00
0.00
RGDL70
0
24
31
1.08
3.23
RGDL71
3
18
40
0.00
0.00
RGDL72
0
24
47
0.00
6.45
RGDL73
1
10
26
12.50
4.17
RGDL74
1
8
30
6.06
3.03
RGDL75
0
48
45
2.97
3.96
RES76
0
4
41
0.00
0.00
RES77
0
22
38
2.00
2.00
RES78
2
16
54
0.00
0.00
RM79
1
7
74
1.01
2.02
RM80
1
2
59
1.94
0.00
RC81
39
39
59
0.00
0.00
RC82
0
32
32
0.00
2.06
RC83
0
0
97
0.00
0.00
RC84
1
37
38
0.00
3.03
RC85
1
81
76
4.90
6.86
RG86
2
3
39
0.00
3.00
RG87
0
1
39
0.99
11.88
RG88
0
0
0
0.00
0.00
RG89
4
61
56
0.00
1.98
RCU90
3
5
75
0.00
1.08
RCU91
0
38
38
1.01
4.04
RCU92
20
43
23
0.00
0.00
RCU93
0
24
50
0.00
3.09
RCU94
1
5
52
0.00
8.82
RCU95
2
98
96
0.00
0.00

-------
Appendix A5 (cont'd)
Site ID PRCNTOLIG PRCNTNINS PRCNTDOMI PRCNTITOL PRCNTPRD
RCU96
1
93
92
0.00
0.00
RGDAN97
0
4
25
11,00
23.00
RGDAN98
9
9
44
6.12
12.24
RGDAN99
0
24
24
1.03
1.03
RGDAN100
2
10
40
4.85
7.77
RGDAN101
3
3
56
4.04
4.04
RGDAN102
3
4
46
2.97
12.87
RG DAN 104
0
53
53
6.25
0.00
RGDAN105
0
77
77
5.00
0.00
RG DAN 106
0
85
85
0.00
0.00
RGDAN107
6
6
38
0.00
1.98

-------
Appendix A5 (cont'd)
SiteJD
PRCNTSCR
PRCNTCF
PR09001
0.00
0.00
PR09001R
0.00
0.00
PR09001D


PR09002
8.47
22.88
PR09003
0.00
14.15
PR09004
0.00
0.00
PR09005
0.00
5.66
PR09006
12.39
9.73
PR09007
0.00
0.00
PR09009
0.00
0.00
PR09010
0.00
1.00
PR09011
6.89
0.86
PR09012
9.91
3.60
PR09013
6.03
33.62
PR09014
0.00
0.00
PR09015
5.04
11.76
PR09016
0.78
6.25
PR09017
0.00
0.00
PR09018
0.00
8.90
PR09020
13.82
21.13
PR09020R
20.00
29.60
PR09020D


PR09021
0.00
0.00
PR09022
0.00
0.00
PR09023
0.00
0.00
PR09024
17.50
17.50
PR09025
0.00
9.90
PR09026
0.00
0.00
PR09028
3.96
14.85
PR09029
2.04
1.02
PR09030
7.01
2.63
PR09031
0.00
3.20
PR09032
2.38
0.00
PR09033
0.00
0.00
PR09034
5.55
12.96
PR09034R
11.76
11.76
PR09034D


PR09035
0.88
0.00
PR09036
0.88
18.58
PR09037
0.00
0.00
PR09040
14.52
17.20
PR09040R
2.38
1.58
PR09040D


PR09041
0.00
0.00
PR09042
2.63
0.00
PR09043
5.00
25.00
PR09046
1.87
0.93
PR09047
33.33
8.77

-------
Appendix A5 (cont'd)

SiteJD

PRCNTSCR
PRCNTCF
PR09049

0.00
0.83
PR09049R
0.00
2.15
PR09049D
•

PR09050

0.00
33.33
PR09051

0.00
2.68
PR09052

0.00
45.19
PR09053

0.00
0.00
PR09054

0.00
10.90
PR09055

0.00
0.00
PR09056

0.83
23.33
PR09057

9.92
4.13
PR09058

17.43
13.76
SiteJD

PRCNTSCR
PRCNTCF
A

8.47
45.76
B

20.18
11.40
C

0.00
0.00
SiteJD

PRCNTSCR
PRCNTCF

1
20
13.13

2
0
15

4
10.1
2.02

5
0
3.19

6
22.55
11.76

7
28.71
11.88

8
4.95
1.98

16
2.97
1.98

17
16.19
0.95

18
2.97
3.96

19
3.5
1.75

20
1.01
0

24
10.63
21.28

25
0.97
0.97

44
21.49
50.46

46
4.76
41.9

47
26.21
7.77

48
8.91
16.83

50
2.97
0
SiteJD

PRCNTSCR
PRCNTCF
RGDAOO

21.57
5.88
RGDA01

5.94
0.00
RGDA02

2.04
58.16
RGDA03

8.16
11.22
RGDA04

10.00
51.00
RGDA05

20.79
13.86
RGDA06

0.00
27.45
RGDA07

7,69
47.44

-------
Appendix A5 (cont'd)
SiteJD
PRCNTSCR
PRCNTCF
RGDA08
6.93
65.35
RGDA09
20.00
5.00
RGDA10
2.97
6.93
RGDA11
8.16
18.37
RGDA13
44.90
0.00
RGDA14
6.25
1.04
RGDA15
2.02
0.00
RGDA16
30.21
5.21
RGDA17
29.70
6.93
RGDA18
19.39
42.86
RGDM19
28.28
0.00
RGDM20
2.04
5.10
RGDM21
1.00
17.00
RGDM22
60.00
1.00
RGDM23
11.11
0.00
RGDM24
5.88
1.96
RGDM25
1.01
27.27
RGDM26
4.00
79.00
RGDM27
0.00
28.42
RGDM28
4.76
2.86
RGDM29
1.02
2.04
RGDM30
4.00
10.00
RLP32
14.85
33.66
RLP33
5.83
41.75
RLP34
2.06
28.87
RLP35
13.13
26.26
RLP36
14.85
20.79
RLP37
11.32
5.66
RLP38
8.91
71.29
RLP39
17.82
14.85
RLP40
9.35
16.82
RLP41
4.04
4.04
RLP42
6.06
33.33
RLP43
3.80
15.19
RLP44
4.72
15.09
RLP45
3.13
5.21
RLP46
10.00
18.00
RLP47
11.11
17.17
RLP48
17.31
3.85
RLP49
9.09
7.07
RDB50
0.00
75.25
RDB51
2.06
20.62
RDB52
20.21
6.38

-------
Appendix A5 (cont'd)
Site J D
PRCNTSCR
PRCNTCF
RDB53
1.04
1.04
RDB54
0.00
0.00
RGDL55
32.00
39.00
RGDL56
1.00
16.00
RGDL57
1.94
70.87
RGDL58
11.11
64.65
RGDL59
3.92
81.37
RGDL60
10.00
9.00
RGDL61
50.50
7.92
RGDL62
8.08
14.14
RGDL63
0.00
48.00
RGDL64
0.00
21.57
RGDL65
2.00
7.00
RGDL66
0.00
0.00
RGDL67
5.94
0.00
RGDL68
2.97
0.99
RGDL69
8.00
0.00
RGDL70
2.15
10.75
RGDL71
0.00
15.46
RGDL72
0.00
0.00
RGDL73
30.21
16.67
RGDL74
8.08
42.42
RGDL75
10.89
3.96
RES76
1.00
33.00
RES77
1.00
6.00
RES78
0.00
13.27
RM79
0.00
4.04
RM80
1.94
5.83
RC81
0.00
0.00
RC82
4.12
13.40
RC83
0.00
0.00
RC84
14.14
0.00
RC85
5.88
0.00
RG86
0.00
10.00
RG87
0.00
6.93
RG88
0.00
0.00
RG89
3.96
1.98
RCU90
0.00
4.30
RCU91
1.01
0.00
RCU92
2.02
0.00
RCU93
0.00
1.03
RCU94
0.00
6.86
RCU95
0.00
1.03

-------
Appendix A5 (cont'd)
SiteJD
PRCNTSCR
PRCNTCF
RCU96
0.00
1.00
RGDAN97
35.00
0.00
RGDAN98
15.31
0.00
RGDAN99
3.09
7.22
RGDAN100
10.68
1.94
RGDAN101
11.11
3.03
RGDAN102
4.95
0.99
RGDAN104
10.42
11.46
RGDAN105
5.00
2.00
RGDAN106
3.03
3.03
RGDAN107
0.00
3.96

-------
Appendix B-l

-------
Appendix Bl: Candidate Metric Distributions (ND
= Insufficient range of metric values)
Forest Cover (High/Low): Score=0
a.
o
w
H
CJ
85% and <30%)
Forest Cover (High/Low): Score=0
g:
i
o
o
DC
CL
120
100 -
80 -


20 -
1	2
Forest (>85% and <30%)
Forest Cover (High/Low): Score=0
LL
o
0
01
CL
100
80 -
60 -
40 -
20 -
Forest (>85% and <30%)
Forest Cover (High/Low): Score=ND
rr 10
I
I
Forest (>85% and <30%)

-------
Appendix B1 cont'd
Forest Cover (High/Low): Score=3
120
W
H
r-
2
O
OH
GL
100 -
80 -
60 -
40 -
20 -
0 -
50
Forest (>85% and <30%)
Forest Cover (High/Low): Score=3
40 -
o
H
H
2
O
cc
a.
30 -
20
10 -
0 -
Forest (>85% and <30%)
Forest Cover (High/Low): Score=0
1	2
Forest (>85% and <30%)
Forest Cover (High/Low): Score=1
Forest (>85% and <30%)

-------
Appendix B1 cont'd
Forest Cover (High/Low): Score=ND
a
in
_i
H
2
O
CL
14
12
10 -
8
6
4
2 -
0 -
1	2
Forest (>85% and <30%)
Forest Cover (High/Low): Score=2
70
60 -
50 ¦
-I 40 ¦
O
O
Z 30 •
o
cc
a 20 ¦
10
0
"X"
i
jH
1	2
Forest (>85% and <30%)
Forest Cover (High/Low): Score=2
o
t-
o
cc
CL
100
80 -
60 -

20 -
Forest (>85% and <30%)
Forest Cover (High/Low): Score=0
Q.
Q
h-
Z
o
a:
CL
120
100 -
80 -


20 -
Forest (>85% and <30%)

-------
Appendix B1 cont'd
Forest Cover (High/Low): Score=0
50
40
2 30
O
Q
O
H 20
O
DC
CL
10
T
o -
1	2
Forest (>85% and <30%)
Forest Cover (Fligh/Low): Score=1
20
18
16
14
12
10
(—
O
H


Forest (>85% and <30%)
Forest Cover (Fligh/Low): Score=3
1	2
Forest (>85% and <30%)
Forest Cover (Fligh/Low): Score=0
10 -
Forest (>85% and <30%)

-------
Appendix B1 cont'd
Forest Cover (High/Low): Score=1
10
2
X
y
cc
>
—1
LL
O
2 -
X
i
1	2
Forest (>85% and <30%)
Forest Cover (High/Low): Score=0
80
60
O 40 -
LL.
o
H
Z
o
£ 20
Forest (>85% and <30%)
Forest Cover (High/Low): Score=1
x
o
o:
i—
LIJ
10 -
Forest (>85% and <30%)
Forest Cover (High/Low): Score=2
100
I—
LU
o
cc
Q.
T
Forest (>85% and <30%)

-------

-------
Appendix B-2

-------
Appendix B2
Index alternatives for Puerto Rico wadeable streams
Index 1
100
80 -
60
40 -
20 -
1	2
Forest Cover (>85% and <15%)
Index 3
1	2
Forest Cover (>85% and <15%)
Index 2
1	2
Forest Cover (>85% and <15%)
Index 4
i
1	2
Forest Cover (>85% and <15%)

-------
Appendix B2 (cont'd)
80
70 H
60
85% and <15%)
Index 7
60 -
O
o
CO
X
0)
"D
c 40

±
20 -
X
Forest Cover (>85% and <15%)
Index 6
100 -
80 -
2 60 -
O
o
V)
X
CD
*o
c 40 -
20 -
0 -
Forest Cover (>85% and <15%)
i
Index 8
100
O
o
(/)
X
0
"Q
c
1
1	2
Forest Cover (>85% and <15%)

-------
Appendix B2 (cont'd)
Index 9
o
o
W
X
85% and <15%)

-------
Appendix C-l

-------
Appendix C1
Spearman rank correlations (p < 0.05) between metrics and water chemistry variables for the 50 sites sampled in 2009
("No" indicates no significant relationship).
totfam taxarich etrich cflyrichn crusrich prcntcfcm prcntet
DO(mg/L)	0,311	0.330	0.410 0.506 No	0.370 No
Temp(.C)	No	No	No No No	No -0.285
PH	No	No	No No No	No No
COND	No	No	No -0.310 No	No No
CA (jig/L)	No	No	No No No	No No
K (Mfl/U	-0.305 No	No	-0.300 No	-0.269 No
MG (\iglL)	No	No	No No No	No 0.294
NA (pg/L)	No	No	No -0.348 No	-0.321 No
ALKALINITY, TOT PPM	No	No	No No No	No No
CL (peq/L)	No	-0.268	-0.403 -0.485 No	-0.426 No
N03N_PPM	No	No	No No No	No No
NTL_PPM	No	No	No No -0,269 No	No
ORTHOPHOSPHATE [AS P] No	No	No	No No	No No
PTL_PPM	No	No	-0.270 No No	No No
S04PPM	No	No	No No No	No No
TOC_PPM	-0.355	-0.336	-0.499 -0.579 No	-0.549 No
DOC_PPM	-0.342	-0.310	-0.460 -0.536 No	-0.501 No
RESIDUE, NON-FILTERABLE	-0.289	-0.331	-0.393 -0.350 No	No No
TURB (NTU)	-0.355	-0.339	-0.410 -0.316 No	-0.268 No
SI02 (ng/L)	No	No	No No No	No No
NITRITE [AS N]	No	No	No No No	No -0.278
AMMONIA [AS N]	-0.312 No	-0.392	-0.431 No	-0.384 No

-------
Appendix C1 (cont'd)
PRCNTLEP PRCNTMFCM PRCNTCOL PRCNTDIP PRCNTGAS PRCNTODON
DO(mg/L)

0.412 No

0.559 No
No
No
Temp(.C)
No

-0.273 No

-0.288
0.610 No
pH
No
No
No
No
No
No
COND

-0.269 No
No
No
No
No
CA(Mg/L)
No
No
No
No
No
No
K (ng/L)
No
No

-0.302
-0.383 No
No
MG (jjg/L)
No

0.336 No
No
No

NA (Jjg/L)
No
No
No
No
No
No
ALKALINITY, TOT_PPM

-0.270 No
No
No
No
No
CL (peq/L)

-0.385 No

-0.364 No
No
No
N03NPPM
No
No
No
No
No
No
NTL_PPM
No
No
No
No
No
No
ORTHOPHOSPHATE [AS P]
No
No
No
No
No
No
PTLPPM
No
No

-0.374 No
No
No
S04_PPM
No
No
No
No
No
No
TOCPPM

-0.279 No

-0.519
No
No
DOC_PPM

-0.308 No

-0.457 No
No
No
RESIDUE, NON-FILTERABLE

-0.484 No
No
No
No

TURB (NTU)
No
No

-0.322 No
No

SI02 (pg/L)
No
No
No
No
No
No
NITRITE [AS N]
No
No

-0.300 No
No
No
AMMONIA [AS N]
No
No

-0.368 No
No

0.387
-0.291
-0.383
-0.306

-------
Appendix C1 (cont'd)
PRCNTCRUS PRCNTCHIR PRCNTOLIG PRCNTNINS PRCNTDOMI PRCNTITOL
DO(mg/L)
No
No
No
No
No

0.419
Temp(.C)
No
No
No

0.414 No
No

PH
No
No
No
No
No
No

COND
No
No
No
No
No
No

CA_PPM
No
No
No
No
No
No

K_PPM
No

-0.290 No
No
No
No

MG_PPM
No
No
No
No
No
No

NA_PPM
No
No

0.309 No
No

-0.331
ALKALINITY, TOT.
No
No
No
No
No
No

CL
No
No

0.317 No
No

-0.360
N03N_PPM
No
No
No
No
No
No

NTL_PPM
No
No
No
No
No
No

ORTHOPHOSPHATE [AS P]
No
No
No
No
No
No

PTL
No
No
No
No
No
No

S04_PPM
No
No
No
No
No
No

TOC
No
No

0.281
0.386
0.424 No

DOC
No
No
No

0.277
0.360 No

RESIDUE, NON-FILTERABLE
No
No

0.305 No
No
No

TURB
No
No

0.372
0.342 No
No

SI02
No
No
No
No
No
No

NITRITE [AS N]
No
No
No
No
No
No

AMMONIA [AS N]
No
No
No

0.300 No
No


-------
Appendix C1 (cont'd)
PRCNTPRD PRCNTSCR PRCNTCF
DO(mg/L)	No	0.499	0.438
Temp(.C)	No	No	-0.353
pH	No	No	No
COND	No	-0.343	-0.382
CA_PPM	No	-0.278	-0.319
K_PPM	No	-0.308	-0.329
MG_PPM	No	No	No
NA_PPM	No	-0.415	-0.310
ALKALINITY, TOT.	No	-0.313 -0.389
CL	No	-0.540	-0.368
N03N_PPM	No	No	No
NTL_PPM	No	No	No
ORTHOPHOSPHATE [AS P] No	No	No
PTL	No	No	No
S04_PPM	No	No	No
TOG	No	-0.576	-0.509
DOC	No	-0.591	-0.494
RESIDUE, NON-FILTERABLE No	-0.480 No
TURB	No	-0.355 No
SI02	No	No	No
NITRITE [AS N]	No	No	No
AMMONIA [AS N]	No	-0.357 -0,293

-------
Appendix C-2

-------
Appendix C2
Spearman rank correlations (p < 0.05) between metrics and physical habitat measures ("No" indicates no significant
relationship).
TOTFAM TAXARICH
XFC_ALG
Filamentous algae areal cover
No
No
XFC_AQM
Aquatic macrophyte areal cover
No
No
XFC_LWD
Large woody debris areal cover
No
No
XFCBRS
Brush and small woody debris areal cover

No
XFC_LTR
Live Trees or Roots
No
No
XFC.OHV
Overhanging vegetation areal cover
No
No
XFCUCB
Undercut bank area! cover
No
No
XFC_RCK
Boulder and rock ledge areal cover

No
XFCHUM
Artifical structure areal cover
No

XFC_ALL
Sum of areai cover from all fish concealment types except algae and aquatic macrophytes

No
XFCBIG
Sum of cover from large wood, boulders, over-hanging banks and human structures

No
XFC_NAT
Sum of cover from large wood, brush, overhanging vegetation, boulders and undercut banks

No
PCT__SA
Substrate % Sand (0,6-2mm)
No
No
XEMBED
Substrate mean embeddness -channel + margin(%)
No
No
XCEMBED
Substrate mean embeddness — mid- channel (%)
No
No
XCL
Riparian canopy (> 5m high) cover -trees > 0.3m DBH

No
XCS
Riparian canopy (> 5m high) cover -trees > or equal to 0.3m DBH

No
XMW
Riparian mid-layer (0.5 to 5m high) woody cover

No
XMH
Riparian mid-layer (0.5 to 5m) herbaceous cover
No
No
XGW
Riparian ground layer (<0.5m high) woody cover

No
XGH
Riparian ground layer (<0.5m high) herbaceous cover
No
No
XGB
Riparian ground-layer (<0.5m high) bare ground cover

No
XC
Riparian canopy cover (XCL +XCS)

No
XM
Riparian mid-layer cover (XMW+XMH)

No
XG
Riparian ground-layer vegetation cover (XGW+XGH)
No
No
XCM
Riparian canopy +mid-layer cover (XC+XM)

No
XCMW
Riparian canopy +mid-layer woody cover (XC+XMW)
No
No
XCMG
Riparian cover, sum of 3 layers (XC+XM +XG)
No
No
XCMGW
Riparian woody cover, sum of 3 layers (XC+XMW +XGW)
No
No

-------
Appendix C2 (cont'd)
ETRICH CFLYRICHN CRUSRICH PRCNTCFCM PRCNTET PRCNTLEP PRCNTMFCM PRCNTCOL
XFC_ALG
No
No
No
No
No
No
No
No
XFC_AQM
No
No
No
No

No

No
XFC_LWD
No
No
No
No
No
No
No
No
XFC_BRS
No
No
No
No
No

No
No
XFC_LTR

No
No

No
No
No
No
XFC_OHV
No
No
No
No
No
No
No
No
XFC_UCB
No

No


No


XFC_RCK
No
No
No
No
No
No
No

XFC_HUM


No

No

No
No
XFC_ALL
No
No
No
No
No
No
No
No
XFC_BIG
No
No
No
No
No
No
No
No
XFC_NAT
No
No
No
No
No
No
No
No
PCT_SA
No
No
No
No
No
No
No
No
XEMBEO
No

No
No
No
No
No
No
XCEMBED
No
No
No
No
No
No
No
No
XCL
No
No
No
No
No

No
No
XCS
No
No
No
No
No
No
No
No
XMW
No
No

No
No
No
No
No
XMH
No
No
No
No
No

No
No
XGW
No
No
No
No
No
No
No
No
XGH
No
No
No
No
No

No
No
XGB
No
No
No
No

No

No
XC
No
No
No
No
No
No
No
No
XM
No
No

No
No
No
No
No
XG
No
No
No
No
No

No
No
XCM
No
No
No
No
No
No
No
No
XCMW
No
No
No
No
No
No
No
No
XCMG
No
No
No
No
No
No
No
No
XCMGW
No
No
No
No
No
No
No
No

-------
Appendix C2 (cont'd)
PRCNTDIP PRCNTGAS PRCNTODON PRCNTCRUS PRCNTCHIR PRCNTOLIG PRCNTNINS
XFC_ALG
No

No
No
No
No
No
XFC_AQM
No
No
No
No
No
No

XFC_LWD
No
No

No
No
No
No
XFC_BRS

No
No
No

No
No
XFC_LTR
No
No
No
No
No
No
No
XFC_OHV
No
No
No
No
No

No
XFC_UCB

No
No
No

No
No
XFC_RCK
No
No
No
No
No

No
XFCJHUM
No
No
No
No
No
No
No
XFC_ALL
No
No
No
No
No
No
No
XFC_BIG
No
No
No
No
No
No
No
XFCNAT
No
No
No
No
No
No
No
PCT_SA
No
No
No
No
No

No
XEMBED
No
No
No
No
No
No
No
XCEMBED
No
No
No
No
No
No
No
XCL
No
No
No
No
No
No
No
XCS
No

No
No
No
No
No
XMW


No

No
No
No
XMH
No

No
No
No
No
No
XGW


No
No

No
No
XGH


No
No

No
No
XGB

No
No
No

No
No
XC
No

No

No
No
No
XM
No
No


No
No
No
XG
No
No
No
No
No
No
No
XCM
No

No

No
No
No
XCMW


No

No
No
No
XCMG
No
No
No

No
No
No
XCMGW


No

No
No
No

-------
Appendix C2 (cont'd)
PRCNTDOMI PRCNTITOL
XFC_ALG
No
No
XFCAQM
No
No
XFC_LWD
No
No
XFC_BRS
No
No
XFC_LTR
No
No
XFC_OHV

No
XFCJJCB

No
XFC_RCK
No
No
XFC_HUM
No
No
XFC_ALL
No
No
XFC_BIG
No
No
XFC_NAT
No
No
PCT_SA
No
No
XEMBED
No

XCEMBED
No

XCL
No
No
XCS
No
No
XMW
No

XMH
No
No
XGW
No

XGH
No
No
XGB
No
No
XC
No
No
XM


XG
No
No
XCM


XCMW
No
No
XCMG


XCMGW
No
No
PRCNTPRD
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
PRCNTSCR
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
PRCNTCF
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No
No

-------