&EPA K^,pr°,ec,,°r Analytical Methods Approved for Compliance Monitoring under the Surface Water Treatment Coliform Rule Analysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent as determined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74(a)(1). Additional methods are listed in Appendix A to Subpart C of Part 141. The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods information published in 40 CFR 141. If discrepancies are found, please notify the Safe Drinking Water Hotline (800- 426-4791) so that EPA can correct the table. §141.74(a)(1)- Analytical and monitoring requirements (a)(1) Analytical requirements (1) The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10 °C during transit. The procedures must be done in accordance with the documents listed at §141.74(a)(1) or one of the alternative methods listed in Appendix A to Subpart C of Part 141. For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of the method listed in §141.74(a)(1) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. The methods listed are the only versions that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods as product packaging inserts may not be the same as the approved versions of the methods. Contaminant Total Coliforms - lactose fermentation methods: Method Organization Reference Title Date Notes 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Office of Water (MS - 4606 M) EPA 821-F-17-002 February 2017 ------- Page 2 of 9 Method Organization Reference Title Date Notes Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison Standard Methods for the demonstrate that the false-positive rate and the false-negative 9221 A, B, C Standard Methods Examination of Water and Wastewater, 19th Edition 1995 rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison Standard Methods for the demonstrate that the false-positive rate and the false-negative 9221 A, B, C Standard Methods Examination of Water and Wastewater, 20th Edition 1998 rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison Standard Methods Standard Methods for the demonstrate that the false-positive rate and the false-negative 9221 A, B, C Examination of Water and Wastewater, 21st Edition 2005 rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Standard Methods for the Lactose broth, as commercially available, may be used in lieu of 9221 A, B, C Standard Methods Examination of Water and Wastewater, 22nd Edition 2012 lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the ------- Page 3 of 9 Method Organization Reference Title Date Notes water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C-99 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1999 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2006 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Total coliforms - membrane filtration methods: Method Organization Reference Title Date Notes 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 ------- Page 4 of 9 Method Organization Reference Title Date Notes 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 9222 A, B, C-97 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 1604 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (Ml Medium), September 2002 2002 Total coliforms - enzyme substrate methods: Method Organization Reference Title Date Notes 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 9223 B Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 ------- Page 5 of 9 Method Organization Reference Title Date Notes 9223 B-97 Colilert Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 9223 B-04 Colilert Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2004 Contaminant Fecal coliforms - Fecal Coliform Procedure (following Lactose Fermentation Methods): Method Organization Reference Title Date Notes 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E-99 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1999 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C ------- Page 6 of 9 Method Organization Reference Title Date Notes 9221 E-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2006 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C Contaminant Fecal coliforms - membrane filtration methods: Method Organization Reference Title Date Notes 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 9222D Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 9222D Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 9222 D-97 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 9222 D-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2006 ------- Page 7 of 9 Contaminant Heterotrophic bacteria - culture method: Method Organization Reference Title Date Notes 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 9215 B-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 9215 B-04 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2004 Contaminant Heterotrophic bacteria - enzyme substrate methods: Method Organization Reference Title Date Notes Simplate IDEXX IDEXX SimPlate™ HPC test method for Heterotrophs in Water, November 2000 2000 ------- Page 8 of 9 Contaminant Turbidity methods: Method Organization Reference Title Date Notes Standard Methods for the Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 2130 B Standard Methods Examination of Water and Wastewater, 18th Edition 1992 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Standard Methods for the Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 2130 B Standard Methods Examination of Water and Wastewater, 19th Edition 1995 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Standard Methods for the Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 2130 B Standard Methods Examination of Water and Wastewater, 20th Edition 1998 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Standard Methods for the Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 2130 B Standard Methods Examination of Water and Wastewater, 21st Edition 2005 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Standard Methods for the Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 2130 B Standard Methods Examination of Water and Wastewater, 22nd Edition 2012 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Methods for the Determination of Inorganic Substances in Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 180.1 EPA Environmental Samples, EPA/600/R-93/100, August 1993 1993 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Great Lakes Instruments Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or Method 2 Great Lakes Instruments Method 2, Turbidity, November 2,1992 1992 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Hach FilterTrak Method 10133, Revision 2.0, Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or 10133 Hach Determination of Turbidity by Laser Nephelometry, January 2000 2000 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or Mitchell Method M5271, Revision 1.1, Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or M5271 Leek Mitchell Determination of Turbidity by Laser Nephelometry, March 5, 2009 2009 equivalent) and stablilized formazin (e.g., Hach StablCal™ equivalent) are acceptable substitutes for formazin or ------- Page 9 of 9 Method Organization Reference Title Date Notes M5331 Leek Mitchell Mitchell Method M5331, Revision 1.1, Determination of Turbidity by LED Nephelometry, March 5, 2009 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin AMI Turbiwell Swan Analytische Instrumente AG Continuous Measurement of Turbity Using ASWAN AMI Turbiwell Turbidimeter, August 2009 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin AQ4500 Thermo Scientific Orion Method AQ4500, Revision 1.0, Determination of Turbidity by LED Nephelometry, May 8, 2009 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin M5331 (Rev. 1.2) Leek Mitchell Mitchell Method M5331, Revision 1.2 Determination of Turbidity by LED or Laser Nephelometry, February 2016 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 10258 Hach Company Hach Method 10258, Determination of Turbidity by 360° Nephelometry, January 2016 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin ------- |